Biuret Reaction: A reaction characterized by a violet color upon the addition of copper sulfate to all compounds with two amide or peptide bonds linked directly or through an intermediate carbon atom. Used in the detection and estimation of proteins and peptides having more than two amino acids.Biuret: Used as feed supplement for sheep and cattle since it is a good non-protein nitrogen source. In strongly alkaline solution biuret gives a violet color with copper sulfate.Allophanate Hydrolase: An enzyme that catalyzes the hydrolysis of allophanic acid to two molecules of ammonia plus two molecules of "active carbon dioxide". EC 3.5.1.54.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.European Union: The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)Blood Volume: Volume of circulating BLOOD. It is the sum of the PLASMA VOLUME and ERYTHROCYTE VOLUME.Erythrocyte Membrane: The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Sodium: A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Potassium: An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Methanosarcina: A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.Methanosarcina barkeri: A species of halophilic archaea whose organisms are nonmotile. Habitats include freshwater and marine mud, animal-waste lagoons, and the rumens of ungulates.Methane: The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)Euryarchaeota: A phylum of ARCHAEA comprising at least seven classes: Methanobacteria, Methanococci, Halobacteria (extreme halophiles), Archaeoglobi (sulfate-reducing species), Methanopyri, and the thermophiles: Thermoplasmata, and Thermococci.Methanosarcinaceae: A family of anaerobic METHANOSARCINALES whose cells are mesophilic or thermophilic and appear as irregular spheroid bodies or sheathed rods. These methanogens are found in any anaerobic environment including aquatic sediments, anaerobic sewage digesters and gastrointestinal tracts. There are four genera: METHANOSARCINA, Methanolobus, Methanothrix, and Methanococcoides.Archaeal Proteins: Proteins found in any species of archaeon.Acetates: Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.Retinoids: A group of tetraterpenes, with four terpene units joined head-to-tail. Biologically active members of this class are used clinically in the treatment of severe cystic ACNE; PSORIASIS; and other disorders of keratinization.Clofibrate: A fibric acid derivative used in the treatment of HYPERLIPOPROTEINEMIA TYPE III and severe HYPERTRIGLYCERIDEMIA. (From Martindale, The Extra Pharmacopoeia, 30th ed, p986)Methylcholanthrene: A carcinogen that is often used in experimental cancer studies.Encephalitis Virus, Murray Valley: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), found in Australia and New Guinea. It causes a fulminating viremia resembling Japanese encephalitis (ENCEPHALITIS, JAPANESE).Phenobarbital: A barbituric acid derivative that acts as a nonselective central nervous system depressant. It potentiates GAMMA-AMINOBUTYRIC ACID action on GABA-A RECEPTORS, and modulates chloride currents through receptor channels. It also inhibits glutamate induced depolarizations.Tretinoin: An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).Carcinoma, Hepatocellular: A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.Liver Neoplasms: Tumors or cancer of the LIVER.Liver Neoplasms, Experimental: Experimentally induced tumors of the LIVER.alpha-Fetoproteins: The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.Hep G2 Cells: A human liver tumor cell line used to study a variety of liver-specific metabolic functions.Millettia: A plant genus of the family FABACEAE. Members contain ISOFLAVONES, some of which show molluscicidal and schistosomicidal activity. Some species of Pongamia have been reclassified to this genus and some to DERRIS.Cell Line, Tumor: A cell line derived from cultured tumor cells.Weight Loss: Decrease in existing BODY WEIGHT.Blood Glucose: Glucose in blood.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Diabetes Mellitus, Type 2: A subclass of DIABETES MELLITUS that is not INSULIN-responsive or dependent (NIDDM). It is characterized initially by INSULIN RESISTANCE and HYPERINSULINEMIA; and eventually by GLUCOSE INTOLERANCE; HYPERGLYCEMIA; and overt diabetes. Type II diabetes mellitus is no longer considered a disease exclusively found in adults. Patients seldom develop KETOSIS but often exhibit OBESITY.Obesity: A status with BODY WEIGHT that is grossly above the acceptable or desirable weight, usually due to accumulation of excess FATS in the body. The standards may vary with age, sex, genetic or cultural background. In the BODY MASS INDEX, a BMI greater than 30.0 kg/m2 is considered obese, and a BMI greater than 40.0 kg/m2 is considered morbidly obese (MORBID OBESITY).Body Weight: The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.Hemoglobin A, Glycosylated: Minor hemoglobin components of human erythrocytes designated A1a, A1b, and A1c. Hemoglobin A1c is most important since its sugar moiety is glucose covalently bound to the terminal amino acid of the beta chain. Since normal glycohemoglobin concentrations exclude marked blood glucose fluctuations over the preceding three to four weeks, the concentration of glycosylated hemoglobin A is a more reliable index of the blood sugar average over a long period of time.Berberine: An alkaloid from Hydrastis canadensis L., Berberidaceae. It is also found in many other plants. It is relatively toxic parenterally, but has been used orally for various parasitic and fungal infections and as antidiarrheal.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Electron Transport Complex I: A flavoprotein and iron sulfur-containing oxidoreductase complex that catalyzes the conversion of UBIQUINONE to ubiquinol. In MITOCHONDRIA the complex also couples its reaction to the transport of PROTONS across the internal mitochondrial membrane. The NADH DEHYDROGENASE component of the complex can be isolated and is listed as EC 1.6.99.3.Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Antioxidants: Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.Mitochondria, Liver: Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)

Standards for total serum protein assays--a collaborative study. (1/13)

We have studied the standardization of total serum protein assay with the biuret reaction. Standard solutions were prepared from lyophilized preparations of human serum albumin and bovine serum albumin, with corrections made for volatile material and ash contents. These solutions and a solution of crystalline albumin standard were analyzed with a new stable biuret reagent, to establish absorptivity values (values for the absorbance of a 1 g/liter final reaction mixture). The mean values obtained were 0.302, 0.292, and 0.290 for human serum albumin, bovine serum albumin, and the crystalline albumin, respectively. We believe that the established absorptivity value will improve the accuracy of serum protein determinations. We studied the linearity of the relation between color produced and protein concentration, with use of the solutions described above and a serum pool. The color adheres to Beer's law up to the highest concentration tested: 3 g/liter for HSA and BSA, and 2.8 g/liter for serum in the final reaction mixture. The new biuret reagent has been stable for one year at room temperature. We recommend the use of bovine serum albumin as a primary standard for serum protein assays. It is inexpensive, easily available, and exhibits the best linearity in the biuret reaction.  (+info)

Fabrication of microchannel structures in fluorinated ethylene propylene. (2/13)

A new technique for fabrication of channel structures with diameters down to 13 microm in fluorinated ethylene propylene (also known as poly(tetrafluoroethylene-co-hexafluoropropylene), FEP) is described. The technique is based on the unique property of a dual-layer fluoropolymer tubing consisting of an outer layer of poly(tetrafluoroethylene) (PTFE) and an inner layer of FEP. When heated (>350 degrees C), the outer PTFE layer shrinks while the inner FEP layer melts, resulting in filling of all empty space inside the tubing with FEP. The channel structures are formed using tungsten wires as templates that are pulled out after completion of the shrinking and melting process. While several analytical devices have been reproducibly prepared and shown to function, this report describes a single example. A microreactor coupled to an electrochemical flow cell detects the biuret complex of the natively electroinactive peptide des-Tyr-Leu-enkephalin.  (+info)

Cross-reactivity of amino acids and other compounds in the biuret reaction: interference with urinary peptide measurements. (3/13)

BACKGROUND: Biuret assays for total protein measurement are considered to react with all peptides longer than 2 residues. Some studies using biuret assays of urine suggest that small peptides generally are more abundant than proteins in urine, but it is not clear whether this is a problem of assay specificity. METHODS: We analyzed the specificity and kinetics of a biuret reaction for solutions of amino acids, organic compounds, peptides, proteins, and ultrafiltered urine specimens and compared the results with standard clinical assays for protein measurement. RESULTS: The biuret assay cross-reacted with several amino acids, dipeptides, and other organic compounds able to form 5- or 6-member ring chelation complexes with copper. Reactions with amino acids and dipeptides had higher absorbance maxima (blue color) than with larger peptides and proteins (purple). Compounds forming potential 4-, 7-, 8-, or 9-member ring complexes with copper had low reactivity. Amino acid amides, dipeptides, and longer peptides had substantial reactivity, except those containing proline. Proteins and polypeptides had similar biuret reactivities per peptide bond, but reaction kinetics were slower for proteins than peptides. Urine specimens ultrafiltered through 3-kDa-cutoff membranes had substantial biuret reactivity, but absorbance maxima were consistent with cross-reactive amino acids rather than peptides. CONCLUSIONS: Many compounds, including amino acids, amino acid derivatives, and dipeptides, cross-react in biuret assays. Our studies improve understanding of the specificity of endpoint and kinetic biuret assays widely used in clinical laboratories. Amino acids, urea, and creatinine contribute to overestimation of urinary peptide content by biuret assays.  (+info)

A novel approach to enhance antibody sensitivity and specificity by peptide cross-linking. (4/13)

 (+info)

The Coomassie Brilliant Blue method underestimates drug-induced tubular proteinuria. (5/13)

The sensitivity of the Coomassie Brilliant Blue (CBB) procedure differs for different proteins. To investigate the impact of this variability on clinical measurements of proteinuria, we collected urine specimens from patients with glomerular disease or drug-induced tubular nephrotoxicity and compared urinary protein concentrations as determined by the CBB method with those obtained by the biuret method. The CBB method underestimated the protein concentration predicted by the biuret method by as much as eightfold in specimens with low proportions of albumin. Determinations of protein by the CBB method in serially diluted urine specimens from patients with tubular damage also deviated from linearity by as much as twofold for protein concentrations less than 1 g/L. We conclude that CBB binding is relatively insensitive for measuring proteins specifically associated with tubular damage and hence may be inappropriate for clinical use.  (+info)

Molar absorptivity and the blank correction factor. (6/13)

In photometry, where both the product formed and one or several reactants absorb light at the same wavelength, the absorbance of the "blank" of the sample at the end of the reaction may be less than that measured at the beginning of the reaction, because of consumption of reactant(s). The blank correction factor for the determined result with one light-absorbing reagent is epsilon P / (epsilon P - epsilon R), where epsilon R and epsilon P are the molar absorptivities of the reagent and the product, respectively. We derived a factor for the case when more than one reagent absorbs light at the same wavelength as the measured product. This factor is independent of the concentration of reagent(s) and can correct the determined result or absorbance for the consumption of light-absorbing reagent(s) during the reaction.  (+info)

A candidate reference method for determination of total protein in serum. I. Development and validation. (7/13)

We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau of Standards) as the standard. At 25 degrees C, color development for 30 or 60 min provides identical serum protein values. Glucose (up to 10 g/L) and bilirubin (up to 300 mg/L) do not interfere. Hemoglobin, at 3 g/L, increases apparent serum protein by 0.4 g/L. The presence of dextran in serum causes easily detected turbidity, but this interference can be eliminated by centrifuging the reaction mixture. Therapeutic concentrations of ampicillin, carbenicillin, penicillin, oxacillin, nafcillin, chloramphenicol, cephalothin, and methicillin in blood do not interfere, nor do triglycerides up to 10 g/L. Within-run and day-to-day standard deviations of the method are 0.1 and 0.4 g/L, respectively.  (+info)

Prevention of interference by dextran with biuret-type assay of serum proteins. (8/13)

In assay of serum proteins by use of the biuret reaction, dextran can cause turbidity by formation of an insoluble complex of dextran with copper and tartrate (or EDTA) in strongly alkaline solution. Whether or not the turbidity occurs depends on the tartrate concentration: turbidity is maximal at about 10 g/L, absent at 20 g/L or more, and only slight and delayed at 4 g/L. Two biuret reagents, containing respectively 5.6 and 22.5 g of tartrate per liter, obviate the interference, but the former is suitable only when a short (5 min) incubation is used. Both reagents show linear calibration curves and yield virtually identical results.  (+info)

*Hans Freeman

"Crystallographic Studies of the Biuret Reaction". Nature. 184 (4687): 707-710. Bibcode:1959Natur.184..707F. doi:10.1038/ ... He received a PhD in 1957 for his work on the structure of biuret hydrate, where he completed most of the calculations for the ... His research group extended its interests beyond purely organic compounds and on to metal complexes (such as copper biuret, one ... Hughes, E. W.; Yakel, H. L.; Freeman, H. C. (1961). "The Crystal Structure of Biuret Hydrate". Acta Crystallogr. 14 (4): 345- ...

*Protein primary structure

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ... A β-elimination reaction also splits the chain, but results in a pyruvoyl group at the new N-terminus. This pyruvoyl group may ... This chemical reaction is called an N-O acyl shift. The ester/thioester bond can be resolved in several ways: Simple hydrolysis ... The hydroxylation reaction is catalyzed by an enzyme that requires ascorbic acid (vitamin C), deficiencies in which lead to ...

*Biuret test

The biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per ... In these tests, the Cu+ formed during the biuret reaction reacts further with other reagents, leading to a deeper color. In the ... The test is named so because it also gives a positive reaction to the peptide-like bonds in the biuret molecule. In this assay ... In a secondary reaction, the copper(II) is reduced to copper(I). Buffers, such as Tris and ammonia interfere with this assay, ...

*Franz Hofmeister

Hofmeister also argued for peptide bonds based on the biuret reaction observed with all proteins but never with free amino ... Since biuret has the formula NH2-CO-NH-CO-NH2, that suggested the presence of similar peptide bonds in proteins. primary ... Consequently, they pull the unfolding reaction. Moreover, they may have direct interactions with some standard hydrophobic ...

*List of organic reactions

... reaction Biuret test Blaise ketone synthesis Blaise reaction Blanc reaction Blanc chloromethylation Bodroux reaction Bodroux- ... reaction Ponzio reaction Prato reaction Prelog strain Prevost reaction Prileschajew reaction Prilezhaev reaction Prins reaction ... Beckwith ring expansion reaction Duff reaction Dutt-Wormall reaction Dyotropic reaction E1cB elimination reaction Eder reaction ... ter Meer reaction Thiele reaction Thiol-yne reaction Thorpe reaction Tiemann rearrangement Tiffeneau ring enlargement reaction ...

*Biuret amidohydrolase

In enzymology, a biuret amidohydrolase (EC 3.5.1.84) is an enzyme that catalyzes the chemical reaction biuret + H2O ⇌ {\ ... The systematic name of this enzyme class is biuret amidohydrolase. This enzyme participates in atrazine degradation. Cook AM, ... displaystyle \rightleftharpoons } urea + CO2 + NH3 Thus, the two substrates of this enzyme are biuret and H2O, whereas its 3 ...

*Millon's reagent

... and so must be confirmed by other tests for proteins such as the biuret test and the ninhydrin reaction. The reagent is made by ...

*Ammeline

... can be synthesized by the pyrolysis of urea or the condensation reaction among 2 moles of dicyandiamide and 1 mole of biuret. 2 ...

*Cyanuric acid amidohydrolase

... biuret + CO2 Thus, the two substrates of this enzyme are cyanuric acid and H2O, whereas its two products are biuret and CO2. ... In enzymology, a cyanuric acid amidohydrolase (EC 3.5.2.15) is an enzyme that catalyzes the chemical reaction cyanuric acid + ...

*Isocyanate

The addition of an isocyanate to a urea gives a biuret: R2NC(O)N(H)R' + R"NCO → R2NC(O)NR'C(O)NHR" Reaction between a di- ... Schmidt reaction, a reaction where a carboxylic acid is treated with ammonia and hydrazoic acid yielding an isocyanate. Curtius ... Isocyanates react with water to form carbon dioxide: RNCO + H2O → RNH2 + CO2 This reaction is exploited in tandem with the ... Isocyanates are common intermediates in the synthesis of primary amines via hydrolysis: Hofmann rearrangement, a reaction in ...

*Potassium cyanate

KOCN is prepared by heating urea with potassium carbonate at 400 °C: 2 OC(NH2)2 + K2CO3 → 2 KOCN + (NH4)2CO3 The reaction ... Intermediates and impurities include biuret, cyanuric acid, and potassium allophanate (KO2CNHC(O)NH2), as well as unreacted ...

*Oxime

... namely the formation of biurets or cyanate polymers., To avoid this side-reaction, strict temperature control is necessary; the ... and a similar reaction with propiophenone, the reaction of phenacyl chloride, the reaction of malononitrile with sodium nitrite ... This reaction is called Beckmann rearrangement. In this reaction, a hydroxyl group is exchanged with the group that is in the ... Examples are the reaction of ethyl acetoacetate and sodium nitrite in acetic acid, the reaction of methyl ethyl ketone with ...

*Chemical test

Sakaguchi test for the presence of arginine in protein Hopkins Cole reaction for the presence of tryptophan in proteins ... sugars Rapid furfural test to distinguish between glucose and fructose The Bicinchoninic acid assay tests for proteins Biuret ... Nitroprusside reaction for the presence of free thiol groups of cysteine in proteins Sullivan reaction for the presence of ... cysteine and cystine in proteins Acree-Rosenheim reaction for the presence of tryptophan in proteins Pauly reaction for ...

*Bicinchoninic acid assay

Biuret test Smith, P.K.; et al. (1985). "Measurement of protein using bicinchoninic acid". Anal. Biochem. 150 (1): 76-85. doi: ... First, the peptide bonds in protein reduce Cu2+ ions from the copper(II) sulfate to Cu+ (a temperature dependent reaction). The ... At higher temperatures (37 to 60 °C), peptide bonds assist in the formation of the reaction complex. Incubating the BCA assay ... Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution ...

*Lowry protein assay

... a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin-Ciocalteu reaction). The reaction mechanism is not well ... Biuret test Bradford protein assay Van Noorden, R.; Maher, B.; Nuzzo, R. (2014). "The top 100 papers". Nature. 514 (7524): 550- ... The Lowry method is based on the reaction of Cu+, produced by the oxidation of peptide bonds, with Folin-Ciocalteu reagent ( ... The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the ...

*Cyanuric acid

"21CFR573.220 Feed-grade biuret". U.S. Food and Drug Administration. April 1, 2006. Retrieved 2007-05-06. "Roughage Buster Plus ... "Study of the thermal decomposition of urea (pyrolysis) reaction and importance to cyanuric acid production," American ... Intermediates in the dehydration include both isocyanic acid, biuret, and triuret: H2N-CO-NH2 → HNCO + NH3 H2N-CO-NH2 + HNCO → ... For example, Archer Daniels Midland manufactures an NPN supplement for cattle, which contains biuret, triuret, cyanuric acid ...

*Jonas Kamlet

And he obtained a patent for the use of biuret for animal feed (Kamlet, 1956). Dow Chemicals bought this patent from Kamlet ... This method utilized a pill containing compounds that dissolved with a strong exothermic reaction without the use of an ... synthesis of biuret that could be used as an element of fodder along with hundreds of other patents. His wife, Edna Yadven ...

*Copper(II) sulfate

... is also used in the Biuret reagent to test for proteins. Copper sulfate is used to test blood for anemia. ... Copper sulfate is often used to demonstrate an exothermic reaction, in which steel wool or magnesium ribbon is placed in an ... In an illustration of a "single metal replacement reaction", iron is submerged in a solution of copper sulfate. Iron reacts ...

*Quantitative proteomics

Absolute quantification is performed using selected reaction monitoring (SRM). MeCAT can be used in combination with element ... Other more accurate spectrophotometric procedures for protein quantification include the Biuret, Lowry, BCA, and Bradford ...

*Arthur W. Thomas

Thomas, A. W. (1913). A further effort to prepare a colorless biuret reagent. Biochemical Bulletin (New York), 2, 556-8. Thomas ... Industrial and Engineering Chemistry, 15, 707-8. Thomas, A. W., & Frieden, A. (1923). The gelatin-tannin reaction. Industrial ...

*Protein adulteration in China

"Feed-grade biuret". Food and Drug Administration. 1 April 2006. Archived from the original on 29 September 2007. Retrieved 6 ... there was a call from consumers for an investigation into Menu Foods reaction to the poisonings, and the federal government's ... NPN are given to ruminants in the form of pelleted urea, ammonium phosphate and/or biuret. Sometimes slightly polymerized ... For example, Archer Daniels Midland manufactures an NPN supplement for cattle, which contains biuret, triuret, cyanuric acid ...
Looking for online definition of biuret reaction in the Medical Dictionary? biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction mean?
Background: Etiological research in schizophrenia is actively exploring biochemical toxic factor that may be result of an innate metabolic error that factor is thought to be abnormal protein factor. Aim: The present study was undertaken to study the concentration of various protein fractions in acute schizophrenics, to find out the association of serum proteins in acute schizophrenia patients and to find out in what fraction of proteins an abnormality lies. Methods: Study consists of two groups, group one formed by healthy individuals, acting as controls and group two by acute schizophrenia patients. With all aseptic precautions, blood was collected and subjected to electrophoresis and for biuret reaction to find out fractional proteins and total proteins respectively. Analysis was done using Students
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A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. Albumin is made mainly in the liver. It helps keep the blood from leaking out of blood vessels. Albumin also helps carry some medicines and...
A total serum protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. Albumin is made mainly in the liver. It helps keep the blood from leaking out of blood vessels. Albumin also helps carry some medicines and...
Immunoglobulin G1 (IgG1) serum concentrations are used to evaluate passive transfer of immunity in neonatal calves. Total serum proteins also can be measured to evaluate calf health. If IgG1 and total serum protein ...
biuret definition: Noun (plural biurets) 1. (organic chemistry) The compound formed by condensation of two molecules of urea with the loss of a molecule of ammonia, (NH2-CO)2NH, or any of its derivatives ...
A positive Biuret test shows a pale to intense violet color when there are two or more peptide bonds in a compound. A negative result, or lack of violet color, indicates either a lack of protein or...
Im doing a practical in biology involving the Biuret, Iodine and Benedicts tests. First I need to predict the following, so any help would be useful ...
This intake and digestion study evaluated source of nonprotein nitrogen in cooked molasses blocks supplemented to 18 steers (590 lb) with ad libitum access to prairie hay, Treatments were 1) control (no block), 2) a block containing 60% crude protein with 83% from urea (UREA block), and 3) a block containing 60% crude protein with 42% from urea and 42% from biuret (UREA/BIURET block). Blocks were broken into small pieces to facilitate rapid consumption and fed once daily at .125% of body weight. Forage intake increased by 22%, total intakes (forage plus block) increased by 28%, and digestible organic matter intakes increased by 52% when either UREA or UREA/BIURET blocks were fed. Total diet digestibilities also increased with block supplementation. Intakes and digestibilities were similar for the UREA and UREA/BIURET blocks. Supplementing prairie hay with cooked molasses blocks containing high levels of nonprotein nitrogen increased intake and digestion, but replacing half of the urea with ...
Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay.. The Biuret assay is a copper ion based protein assay, protein solutions are mixed with an alkaline solution of copper salt, cupric ions (Cu2+). The protein assay is based on the interaction of cupric ions with protein in an alkaline solution. The interaction of cupric ions (Cu2+) with protein results in a purple color that can be read at 545nm. The amount of color produced is proportional to protein ...
Introduction. Chapter 1 ? Chemistry of Life Determining the Presence of Certain Nutrients Kieun Bae, DP2 - Biology HL Introduction The idea of this experiment was to plan the diet for a day and determine if the diet plan contains the necessary nutrients, by testing the food products with chemicals. In this way, we would have better idea about what kind of food product should be included in the daily diet. The meal-plan of the day we have made was following: 1. Breakfast 1. milk 2. cereal 1. Lunch 1. bread 2. juice 3. cheese 4. apple 1. Dinner 1. pasta 2. lattice 3. meat 4. tomato 5. yogurt 6. red wine 7. onion This was planned by discussing the actual food products we commonly take in a day. There are few diary products that are commonly known to contain a lot of protein, necessary carbohydrates for each meal (bread, pasta and cereal) as well as the vegetables that contain many various healthy nutrients including glucose (simple sugar), vitamins, minerals etc. Also, there is meat that is ...
Five steers (491 ± 21 kg BW) were used in an incomplete 5 × 4 Latin square with four 24-d periods to determine the influence of supplemental non-protein N (NPN) source and supplementation frequency (SF) on nutrient intake and site of digestion in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control and a urea- or biuret-containing supplement placed directly into the rumen daily (D) or every-other-day (2D) at 0700. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. Daily TRT were supplemented CP at 0.04% of BW/d while the 2D TRT were supplemented at 0.08% of BW every-other-day. Therefore, all supplemented TRT received the same quantity of supplemental CP over a 2-d period. Forage OM intake was not affected (P , 0.05) by NPN supplementation, NPN source, or SF. However, total OM and N intake were increased (P , 0.01). wi ...
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes. Extinguishing media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam ...
Proteins are extremely important biological molecules, both structurally (e.g. your fingernails are made of the protein keratin, whilst collagen is a very important protein in connective tissue) and functionally as enzymes catalyzing biological reactions. In part A of this prac, students determine the amount of protein in a sample, by effectively measuring the peptide bonds (as each amino acid has a peptide bond connecting it with the next) using a Biuret spectrophotometric assay. Students generate a standard curve, an extremely important tool in biochemistry.. In the second part of the prac, students do a hypothetical experiment on enzymes. Enzymes are protein molecules which act as catalysts of biochemical reactions. Enzymes act by binding the substrate (substance which reacts) and providing a pathway for the reaction, making the conversion from substrate to product faster. Without enzymes, biochemical reactions would take hours or days to proceed. During an enzymatic reaction, substrate is ...
Tratamentul termic al boabelor de soia reduce activitatea inhibitorilor tripsinei si astfel mbunatateste digestibilitatea proteinei. ncalzirea excesiva poate sa distruga sau sa reduca biodisponibilitatea unor aminoacizi termolabili si astfel sa reduca valoarea nutritiva a proteinei din soia. Cercetarile s-au efectuat pe soia toastata si soia netratata termic (granulatie 200 μ), care au fost ncalzite ntr-o etuva cu circulatie fortata a aerului, la temperatura de 120 C, perioade diferite de timp: 5, 10, 15, 20, 25 si 30 minute. S-au determinat indicele de ureaza (UI), solubilitatea proteinei n KOH si indicele de dispersabilitate a proteinei (PDI). Proteina s-a determinat prin metoda biuret. Cercetarile au evidentiat ca indicele de ureaza este util pentru a determina daca faina de soia a fost tratata termic suficient pentru a reduce factorii antinutritionali, dar nu este util pentru determinarea supraprocesarii. Acest test nu este relevant pentru evidentierea ncalzirii excesive, deoarece indicele ...
I am 45 years old male. I had a routine CBC done a few weeks ago, the results showed a slightly low iron count (14.0?). (My doctor says they like to be at 15.0). He said everything else was came back normal. I had a second blood test for ferritin, b12 and folic acid. My doctor says everything came back normal except I am slightly high on total serum protein (8.3?). (He says they like to see a 7.5). According to my studies on the internet, both percentages fall within the normal perameters. He also had me do a urine test (the one with checking under micoscope?).....it came back with no protein (completely clean is what I was told) in the urine ...
When filtered alkaline extracts of pulverized bacteria of several varieties are precipitated with acid in the cold, boiled with acid, and all materials thrown down by these procedures removed, there remains a small amount of an alcohol-precipitable material which no longer gives any of the ordinary chemical reactions for proteins, such as the biuret, Hopkins-Cole, Millon, and sulfosalicylic acid reactions. The only protein reaction usually given by this material is a very weak xanthoproteic reaction. Nevertheless, the material, which is, as far as we can determine at present, free from coagulable protein, is specifically precipitable by homologous antiserum and gives specific complement fixation reactions.. Such material can also be obtained from organisms like the influenza bacillus, pneumococcus, and meningococcus by extraction without preliminary grinding of the bacteria, and is present in filtrates of young and old broth cultures of the organisms.. We believe that these acid- and ...
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
The effects of systemic sclerosis can range from only minimal skin changes to a rapidly progressive course that involves multiple organs and causes early death. It clearly is of great value to physicians to have reliable predictors for patients who may have a poor prognosis. This major multicenter study is prospective, has a wide geographic representation, uses uniform classification and diagnostic criteria, and has a long-term follow-up. Appropriate statistical methods used are used. A total of 484 information items were obtained on each patient, and the paper details 72 selected variables subdivided into 9 appropriate subsets. The data clearly show that the patients with the worst survival were those over 64 years of age with renal disease and younger patients with anemia. Other predictors included severe pulmonary disease and reduced total serum protein levels. These observations alert the physician to target studies at the function of the kidney, lung, heart, and hemopoietic systems. It ...
SDS-PAGE analysis of recombinant VpBD.After electrophoresis, the gel was visualized by Coomassie brilliant blue R250 staining. Lane M: protein molecular standar
Note: ET Group memproduksi beberapa test kit analisis mutu pangan bermerk Easy Test dengan jenis varian antara lain Test Kit Formalin, Test Kit Boraks, Test Kit Methanil Yellow, Test Kit Rhodamine B, Test Kit Mutu Pangan 4 Varian, Test Kit Formalin Paket Industri, TEST KIT MUTU PANGAN 4 VARIAN (PAKET INDUSTRI), Test Kit Sianida, Test Kit Peroksida, Test Kit Hipoklorit (Kaporit), Test Kit Siklamat, Test Kit Sakarin, Test Kit Asam Salisilat, Test Kit Alkalinitas (Alkalinity), Test Kit Asam Sorbat, Test Kit Benzoat, Test Kit Oksalat (Oxalate), Test Kit Tiosianat (Thiocyanate), Test Kit Nitrit, Test Kit Iodat, Test Kit Oksalat, Test Kit Potassium Bromate (Kalium Bromat) dan macam-macam test kit lainnya ...
This study was aimed at investigating the use of seminal plasma protein concentration to predict fertility and hatchability in the domestic fowl. Forty cocks with acceptable sperm motility and morphology were used and the seminal plasma protein concentration determined by the Biuret method. The result revealed a negative significant correlation between the seminal plasma protein concentration with sperm motility (r = -0.5920, ...
EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself.
Hypoproteinemia: A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, EDEMA, or PROTEINURIA.
A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, edema, or proteinuria.
One of the tests performed at the Department of Biologicals of the Laboratory for Medicins and Medical Devices is an assay for the detection of residual animal serum albumin (bovine serum albumin and ovalbumin) in vaccines. The method used is an immunoprecipitation test using Counter Current Electroforesis (C.C.E.). After electroforesis the precipitate of albumin and anti-albumin serum is stained by Coomassie Brilliant Blue. Traditionally the agarose-gels are visually inspected for the presence of stained precipitates using the light of a bulb shining through opal glass. This report describes the validation of a new detection technique. The agarose-gel is illuminated using two halogen lamps. Under this illumination the precipitates appear as little red lines.Using the traditional detection-method (light bulb) the detection-limit was 1 mul animal serum per litre vaccine. Using the new detection-method (halogen-lamps) the detection-limit is lowered by at least a factor 2. Furthermore, as a ...
In the quantitative analysis of a sample by computing constituents from optical absorptivity data at different wavelengths, optical absorptivity data values for selected nominal wavelengths are obtained by curvilinear interpolation from measured optical absorptivity data values obtained by use of narrow band width filters. The computed optical data values are utilized to compute percentage constituents by means of algorithms which may include one or more first or second derivatives.
Penelitian saat ini mengarah untuk menghasilkan suatu immun yang tinggi, secara genetic dimodifikasi pada redbelly tilapia, Tilapia Zillii dengan pertumbuhan dipercepat sebagai hasil injeksi (suntikan) langsung dari DNA ikan hiu (Squalus acanthias L.) ke dalam daging otot benih ikan (fingerlings) pada konsentrasi 10, 20, 40 dan 80 µg/fish. Hasil menunjukkan bahwa ikan yang disuntik dengan 40 µg/fish mempunyai pengaruh keunggulan signifikan (P≤0.05) dari pencapaian pertumbuhan. Juga, komposisi tubuh dari ikan ini telah ditingkatkan. Ikan yang disuntik dengan 20 dan 40 µg/fish pengaruh yang signifikan (P≤0.05) rata-rata lebih tinggi dari total aktivitas antibodi (total IgM), total serum protein dan globulin bandingkan dengan kelompok yang disuntik lainnya. Pengujian cytological untuk semua ikan yang disuntik dan kontrol mengungkapkan jumlah diploid yang sama (2n=44) dari khromosom, dan tidak ada kerusakan khromosomal yang dideteksi. Lebih dari itu, fingerprinting (sidik) DNA menunjukkan ...
For whole cell protease treatment method, E. coli cells have been harvested, washed and resuspended in one ml Tris HCl. Proteinase K was additional to final concentrations in between 0. two mg mL 1 and 0. 5 mg mL 1 and cells had been incubated for one hour at 37 C. Digestion was stopped by washing the cells twice with Tris HCl containing 10% fetal calf serum and outer membrane proteins were ready as described over. For outer membrane proteins that have been applied for ac tivity assays, cells werent handled with Proteinase K. SDS Page Outer membrane isolates were diluted with sam ple buffer containing 4% SDS, 0. 2% bromophenol blue, 200 mM dithiothreitol and 20% glycerol boiled for 10 minutes and analyzed on 10% polyacrylamid gels. Proteins had been stained with Coomassie brilliant blue.. To correlate molecu lar masses of protein bands of curiosity, a molecular fat conventional was employed. Flow cytometer evaluation E. coli BL21 pAT kinase inhibitor MLN0128 LipBc cells were grown and ex ...

Biuret reaction | definition of biuret reaction by Medical dictionaryBiuret reaction | definition of biuret reaction by Medical dictionary

... biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction ... Looking for online definition of biuret reaction in the Medical Dictionary? ... Biuret reaction , definition of biuret reaction by Medical dictionary https://medical-dictionary.thefreedictionary.com/biuret+ ... biuret reaction. Also found in: Dictionary, Encyclopedia. biuret. [bi´u-ret] a urea derivative; its presence is detected after ...
more infohttps://medical-dictionary.thefreedictionary.com/biuret+reaction

IUCr) Acta Crystallographica Volume 20, Part 2, February 1966IUCr) Acta Crystallographica Volume 20, Part 2, February 1966

Crystallographic studies of the biuret reaction. II. Structure of bis-biuret-copper(II) dichloride, Cu(NH2CONHCONH2)2C12. ...
more infohttp://journals.iucr.org/q/issues/1966/02/00/

1911 Encyclopædia Britannica/Albumin - Wikisource, the free online library1911 Encyclopædia Britannica/Albumin - Wikisource, the free online library

The decomposition products are generally the same as with the general albumin; it gives the biuret reaction; forms salts with ... biuret reaction). Boiling with dilute mineral acids, or baryta water, decomposes albumins into carbon dioxide, ammonia and ... They give the biuret and xanthoproteic reactions, and form salts with both acids and bases. Albumoses and peptones are obtained ... They are loose, white, non-hygroscopic powders, soluble in water and salt solutions, and have an acid reaction; they give the ...
more infohttps://en.wikisource.org/wiki/1911_Encyclop%C3%A6dia_Britannica/Albumin

Internet Scientific PublicationsInternet Scientific Publications

Gurnall, A. G; Bardawill, C. T; and David M. M. (1949). Determination of serum protein by means of biuret reaction. J. Biol ... Protein content was determined by the Biuret reaction 26 while lipoprotein lipase and triglyceride lipase activities were ... Searcy, R. L; and Berquist, L. M. (1960): A new colour reaction for the quantification of serum cholesterol. Clin. Chim. Acta, ... Glomset, J.A. (1968): The Plasma Lecithin: Cholesterol acyl transferase reaction. J. Lipid Res, 9:155-167.. 12. Nordby, G; and ...
more infohttp://ispub.com/IJAM/5/2/12643

Sensors  | Free Full-Text | Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as...Sensors | Free Full-Text | Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as...

Figure 9. Total content of proteins soluble in water determined spectrometrically using biuret reaction. The dependence of ... Figure 9. Total content of proteins soluble in water determined spectrometrically using biuret reaction. The dependence of ... ions the changes were observable also in total content of proteins determined using biuret reaction. For this purpose automatic ... The measurement was done as follows: 180 μl of the biuret solution was mixed with 45 μl of real or standard sample; after ...
more infohttp://www.mdpi.com/1424-8220/8/1/445/htm

MrpA Functions in Energy Conversion during Acetate-Dependent Growth of Methanosarcina acetivorans | Journal of BacteriologyMrpA Functions in Energy Conversion during Acetate-Dependent Growth of Methanosarcina acetivorans | Journal of Bacteriology

Determination of serum proteins by means of the Biuret reaction. J. Biol. Chem. 177:751-766. ... a reaction catalyzed by a membrane complex (Mtr). The exothermic reaction producing CH3-S-CoM is coupled to generation of a Na+ ... Valinomycin was added to a final concentration of 50 μM, and after 2 min the reaction was stopped by addition of precooled ... Protein was determined by the Biuret method (32) using bovine serum albumin as the standard. The methane concentration was ...
more infohttps://jb.asm.org/content/195/17/3987?ijkey=ab191ad97e0f31c700c2d6e06df3089dbee609f8&keytype2=tf_ipsecsha

Plus itPlus it

... based on the biuret reaction assay (Doumas et al., 1981). The absorbance of standards and samples was measured at 595 nm. P450 ... After the reaction had been started (in the case of P450, by addition of NADPH-generating solution; in the case of GT, by ... After 30, 20, 20, 6, or 15 min, respectively, each reaction was stopped by addition of 1 ml of ice-cold ethanol. ... After 30, 60, 60, 20, or 15 min, respectively, the reaction was stopped by addition of 1 ml of ice-cold ethanol. ...
more infohttp://dmd.aspetjournals.org/content/26/3/234

Hans Freeman - WikipediaHans Freeman - Wikipedia

"Crystallographic Studies of the Biuret Reaction". Nature. 184 (4687): 707-710. Bibcode:1959Natur.184..707F. doi:10.1038/ ... He received a PhD in 1957 for his work on the structure of biuret hydrate, where he completed most of the calculations for the ... His research group extended its interests beyond purely organic compounds and on to metal complexes (such as copper biuret, one ... Hughes, E. W.; Yakel, H. L.; Freeman, H. C. (1961). "The Crystal Structure of Biuret Hydrate". Acta Crystallogr. 14 (4): 345- ...
more infohttps://en.wikipedia.org/wiki/Hans_Freeman

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Gornall AG, Bardawill CJ, David MM: Determination of serum proteins by means of the biuret reaction. J Biol Chem177 :751 -766, ... The reactions were carried out at 30°C in 1 ml of the reaction medium with 0.8 mg of protein. Homogenates were incubated with ... Reactions were carried out in a chamber with magnetic stirring in 1 ml of reaction medium (100 mmol/l sucrose, 100 mmol/l KCl, ... ΔΨm was estimated from the decrease of TPP+ concentration in the reaction medium as described elsewhere (27). Homogenates were ...
more infohttp://diabetes.diabetesjournals.org/content/52/6/1449

US5478748A - Protein assay using microwave energy 
        - Google PatentsUS5478748A - Protein assay using microwave energy - Google Patents

... such as those based on the reaction with alkaline copper tartrate can also be significantly reduced by exposing the sample and ... The standard (non-microwave) incubation time for a Biuret reaction based protein assay is 30 min; a microwave-Biuret reaction ... Both the BCA and Lowry assays are extensions of the Biuret reaction described by Gornall et al. in the J.Biol.Chem. 177:751 ( ... most preferably a BCA assay but also either a Lowry assay or other assays based upon the Biuret reaction. The apparatus of the ...
more infohttps://patents.google.com/patent/US5478748A/en

US2758040A - Process for producing colorless sterile invert sugar solution 
        - Google PatentsUS2758040A - Process for producing colorless sterile invert sugar solution - Google Patents

The determination of small quantities of bacteria by means of the biuret reaction. ... A spectrophotometric study on the orcinol reaction with carbohydrates. Folin 1919. Laboratory Manual of Biological Chemistry: ... the acid or any reaction product thereof remaining in the solutions after hydrolysis and sterilization be non-toxic. When an ...
more infohttps://patents.google.com/patent/US2758040A/en

Frontiers | Oxidative Stress Function in Women over 40 Years of Age, Considering Their Lifestyle | EndocrinologyFrontiers | Oxidative Stress Function in Women over 40 Years of Age, Considering Their Lifestyle | Endocrinology

Determination of serum proteins by means of the biuret reaction. J Biol Chem (1949) 177(2):751-66. ... Total protein concentration was measured in plasma according to biuret method by spectrophotometry, using serum albumin as the ...
more infohttps://www.frontiersin.org/articles/10.3389/fendo.2017.00048/full

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Gornall AG, Bardawill CJ, and David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177: ... were incubated in 1 ml of reaction media (135 mM sucrose, 65 mM KCL, 2.5 mM MgCl2, 5 mM KH2PO4), pH 7.2. Reaction was initiated ... Mitochondria (1.5 of mg protein) were incubated in 1 ml of reaction media (125 mM sucrose, 65 mM KCL, 2.5 mM MgCl2, 5 mM KH2PO4 ... Reaction was started by 3 μM ATP. Oligomycin was used as a selective inhibitor of ATP synthase (FO unit). ...
more infohttp://jpet.aspetjournals.org/content/323/2/636

Protein primary structure - WikipediaProtein primary structure - Wikipedia

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ... A β-elimination reaction also splits the chain, but results in a pyruvoyl group at the new N-terminus. This pyruvoyl group may ... The hydroxylation reaction is catalyzed by an enzyme that requires ascorbic acid (vitamin C), deficiencies in which lead to ... Such reactions are catalyzed by kinases and the reverse reaction is catalyzed by phosphatases. The phosphorylated tyrosines are ...
more infohttps://en.m.wikipedia.org/wiki/Protein_primary_structure

Protein primary structure - WikipediaProtein primary structure - Wikipedia

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ... A β-elimination reaction also splits the chain, but results in a pyruvoyl group at the new N-terminus. This pyruvoyl group may ... The hydroxylation reaction is catalyzed by an enzyme that requires ascorbic acid (vitamin C), deficiencies in which lead to ... Such reactions are catalyzed by kinases and the reverse reaction is catalyzed by phosphatases. The phosphorylated tyrosines are ...
more infohttps://en.m.wikipedia.org/wiki/Peptide_sequence

Nicotinamide reverses behavioral impairments and provides neuroprotection in 3˗nitropropionic acid induced animal model...Nicotinamide reverses behavioral impairments and provides neuroprotection in 3˗nitropropionic acid induced animal model...

Gornall AG, Bardawill CJ, David MM (1949) Determination of serum proteins by means of the biuret reaction. J Biol Chem 177:751- ...
more infohttps://rd.springer.com/article/10.1007%2Fs11011-018-0297-0

What is the meaning of an investigatory project? | Reference.comWhat is the meaning of an investigatory project? | Reference.com

What is the protein and biuret colour change reaction?. * Q: What is the meaning of a partial curl-up and trunk lift?. ...
more infohttps://www.reference.com/science/meaning-investigatory-project-7e9555e2d70bee75
  • The biuret reaction commonly has been considered to selectively measure peptides 3 amino acid residues in length or longer (3), although there have been reports extending back many years indicating that amino acids, dipeptides, and other compounds are reactive (30-33). (thefreedictionary.com)
  • The diketotetrahydrotriazoles, or urazoles, are formed by condensing urea derivatives with hydrazine salts, urazole itself resulting by the action of urea or biuret on hydrazine or its salts. (yourdictionary.com)
  • The heterodisulfide CoM-S-S-CoB, a product of the CH 3 -S-CoM demethylation reaction, is reduced to the active sulfhydryl forms of the cofactors by heterodisulfide reductase (HdrDE). (asm.org)
  • The Cdh transfers the methyl group to tetrahydrosarcinapterin (THSPT), followed by transfer from CH 3 -THSPT to coenzyme M (HS-CoM), a reaction catalyzed by a membrane complex (Mtr). (asm.org)