Biuret: Used as feed supplement for sheep and cattle since it is a good non-protein nitrogen source. In strongly alkaline solution biuret gives a violet color with copper sulfate.Biuret Reaction: A reaction characterized by a violet color upon the addition of copper sulfate to all compounds with two amide or peptide bonds linked directly or through an intermediate carbon atom. Used in the detection and estimation of proteins and peptides having more than two amino acids.Allophanate Hydrolase: An enzyme that catalyzes the hydrolysis of allophanic acid to two molecules of ammonia plus two molecules of "active carbon dioxide". EC 3.5.1.54.Triazines: Heterocyclic rings containing three nitrogen atoms, commonly in 1,2,4 or 1,3,5 or 2,4,6 formats. Some are used as HERBICIDES.Colorimetry: Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Enterobacter cloacae: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in water, sewage, soil, meat, hospital environments, and on the skin and in the intestinal tract of man and animals as a commensal.Chemistry, Organic: The study of the structure, preparation, properties, and reactions of carbon compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Organic Chemistry Phenomena: The conformation, properties, reaction processes, and the properties of the reactions of carbon compounds.Therapeutic Equivalency: The relative equivalency in the efficacy of different modes of treatment of a disease, most often used to compare the efficacy of different pharmaceuticals to treat a given disease.Color: The visually perceived property of objects created by absorption or reflection of specific wavelengths of light.Gentian Violet: A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.Cross-Over Studies: Studies comparing two or more treatments or interventions in which the subjects or patients, upon completion of the course of one treatment, are switched to another. In the case of two treatments, A and B, half the subjects are randomly allocated to receive these in the order A, B and half to receive them in the order B, A. A criticism of this design is that effects of the first treatment may carry over into the period when the second is given. (Last, A Dictionary of Epidemiology, 2d ed)Iodine: A nonmetallic element of the halogen group that is represented by the atomic symbol I, atomic number 53, and atomic weight of 126.90. It is a nutritionally essential element, especially important in thyroid hormone synthesis. In solution, it has anti-infective properties and is used topically.Iodine Compounds: Inorganic compounds that contain iodine as an integral part of the molecule.Flow Injection Analysis: The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.ArtPaintingsAnimal Feed: Foodstuff used especially for domestic and laboratory animals, or livestock.Rumen: The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)Digestion: The process of breakdown of food for metabolism and use by the body.Dietary Supplements: Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.Poaceae: A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.Respiratory Protective Devices: Respirators to protect individuals from breathing air contaminated with harmful dusts, fogs, fumes, mists, gases, smokes, sprays, or vapors.Skating: Using ice skates, roller skates, or skateboards in racing or other competition or for recreation.Protective Clothing: Clothing designed to protect the individual against possible exposure to known hazards.National Institute for Occupational Safety and Health (U.S.): An institute of the CENTERS FOR DISEASE CONTROL AND PREVENTION which is responsible for assuring safe and healthful working conditions and for developing standards of safety and health. Research activities are carried out pertinent to these goals.Burns, ChemicalAnomura: An infraorder of CRUSTACEA, in the order DECAPODA comprising the hermit crabs and characterized by a small fifth pair of legs.Yogurt: A slightly acid milk food produced by fermentation due to the combined action of Lactobacillus acidophilus and Streptococcus thermophilus.Bread: Baked food product made of flour or meal that is moistened, kneaded, and sometimes fermented. A major food since prehistoric times, it has been made in various forms using a variety of ingredients and methods.Cheese: A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.Onions: Herbaceous biennial plants and their edible bulbs, belonging to the Liliaceae.Bromates: Negative ions or salts derived from bromic acid, HBrO3.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Dirofilaria immitis: A filarial parasite primarily of dogs but occurring also in foxes, wolves, and humans. The parasite is transmitted by mosquitoes.Immunochromatography: A type of affinity chromatography where ANTIBODIES are used in the affinity capture reaction on the solid support, in the mobile phase, or both.Potassium Compounds: Inorganic compounds that contain potassium as an integral part of the molecule.Hydroxides: Inorganic compounds that contain the OH- group.Hydrogen: The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.Potassium: An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.Gases: The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)Dermatomycoses: Superficial infections of the skin or its appendages by any of various fungi.Molluscum Contagiosum: A common, benign, usually self-limited viral infection of the skin and occasionally the conjunctivae by a poxvirus (MOLLUSCUM CONTAGIOSUM VIRUS). (Dorland, 27th ed)Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Buffers: A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

Daily and alternate day supplementation of urea or biuret to ruminants consuming low-quality forage: I. Effects on cow performance and the efficiency of nitrogen use in wethers. (1/12)

Two experiments were conducted to determine the influence of supplemental nonprotein N (NPN) provided daily (D) or every other day (2D) on ruminant performance and N efficiency. Treatments included an unsupplemented control (CON) and a urea (28.7% CP) or biuret (28.6% CP) supplement provided D or 2D at 0700. In Exp. 1, five wethers (39 +/- 1 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental NPN source and supplementation frequency (SF) on the efficiency of N use in lambs consuming low-quality grass straw (4% CP). The amount of CP supplied by each supplement was approximately 0.10% of BW/d (averaged over a 2-d period). In Exp. 2, 80 Angus x Hereford cows (540 +/- 8 kg BW) in the last third of gestation were used to determine the effect of NPN source and SF on cow performance. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. The supplemented treatments received the same amount of supplemental N over a 2-d period; therefore, the 2D treatments received double the quantity of supplemental N on their respective supplementation day than the D treatments. In Exp. 1, total DM, OM, and N intake; DM, OM, and N digestibility; N balance; and digested N retained were greater (P < 0.03) for supplemented than for CON wethers, with no difference (P > 0.05) between NPN sources or SF. Plasma urea-N (PUN) was increased with N supplementation compared with CON (P < 0.01), and urea treatments had greater PUN than biuret (P < 0.01). In addition, PUN was greater (P = 0.02) for D than for 2D treatments. In Exp. 2, pre- and postcalving (within 14 d and 24 h after calving, respectively) cow weight and body condition score change were more positive (P < 0.05) for supplemented groups than for CON. These results suggest that supplements containing urea or biuret as the primary source of supplemental N can be effectively used by lambs and cows consuming low-quality forage, even when provided every other day.  (+info)

Daily and alternate-day supplementation of urea or biuret to ruminants consuming low-quality forage: II. Effects on site of digestion and microbial efficiency in steers. (2/12)

Five steers (491 +/- 21 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental non-protein N (NPN) source and supplementation frequency (SF) on nutrient intake and site of digestion in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control and a urea- or biuret-containing supplement placed directly into the rumen daily (D) or every other day (2D) at 0700. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. Daily TRT were supplemented CP at 0.04% of BW/d, whereas the 2D TRT were supplemented at 0.08% of BW every other day. Therefore, all supplemented TRT received the same quantity of supplemental CP over a 2-d period. Forage OM intake was not affected (P > 0.05) by NPN supplementation, NPN source, or SF; however, total OM and N intake were increased (P < 0.01) with CP supplementation. Duodenal flow of N was greater (P = 0.04) with CP supplementation compared with the control. In addition, duodenal bacterial N flow was increased with CP supplementation (P = 0.04) and for biuret compared with urea (P < 0.01). Bacterial efficiency (g bacterial N/kg OM truly digested in the rumen) was greater (P = 0.05) for biuret than for urea. Apparent total-tract N digestibility was increased with NPN supplementation (P < 0.01) but not affected by NPN source or SF. These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality forage.  (+info)

Daily and alternate-day supplementation of urea or biuret to ruminants consuming low-quality forage: III. Effects on ruminal fermentation characteristics in steers. (3/12)

Five ruminally and duodenally cannulated steers (491 +/- 21 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental nonprotein N (NPN) source and supplementation frequency (SF) on the dynamics of ruminal fermentation in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control (CON) and a urea or biuret supplement that were placed directly into the rumen at 0700 daily (D) or every other day (2D). The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement; therefore, the urea and biuret treatments received the same amount of supplemental N over a 2-d period. Daily TRT were supplemented with CP at 0.04% of BW/d, whereas the 2D TRT were supplemented at 0.08% of BW every other day. Forage was provided at 120% of the previous 5-d average intake in two equal portions at 0715 and 1900. Ruminal fluid was collected 0, 3, 6, 9, 12, and 24 h after supplementation on a day of and a day before supplementation for all TRT. Ruminal NH3-N increased (P < 0.04) with CP supplementation on the day all supplements were provided and on the day on which only daily supplements were provided compared with the CON. However, an NPN source x SF interaction (P = 0.03) on the day all supplements were provided indicated that NH3-N increased at a greater rate for urea as SF decreased compared with biuret. Ruminal NH3-N on the day only daily supplements were provided was greater (P = 0.02) for D compared with 2D. On the day all supplements were provided, D increased (P = 0.05) ruminal indigestible acid detergent fiber passage rate and ruminal fluid volume compared with 2D. These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality forage without adversely affecting ruminal fermentation, even when provided every other day.  (+info)

Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth. (4/12)

TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogeneity. Native TrzF had a subunit molecular weight of 65,401 and a subunit stoichiometry of alpha(2) and did not contain significant levels of metals. TrzF showed time-dependent inhibition by phenyl phosphorodiamidate and is a member of the amidase signature protein family. TrzF was highly active in the hydrolysis of allophanate but was not active with urea, despite having been previously considered a urea amidolyase. TrzF showed lower activity with malonamate, malonamide, and biuret. The allophanate hydrolase from Pseudomonas sp. strain ADP, AtzF, was also shown to hydrolyze biuret slowly. Since biuret and allophanate are consecutive metabolites in cyanuric acid metabolism, the low level of biuret hydrolase activity can have physiological significance. A recombinant Escherichia coli strain containing atzD, encoding cyanuric acid hydrolase that produces biuret, and atzF grew slowly on cyanuric acid as a source of nitrogen. The amount of growth produced was consistent with the liberation of 3 mol of ammonia from cyanuric acid. In vitro, TrzF was shown to hydrolyze biuret to liberate 3 mol of ammonia. The biuret hydrolyzing activity of TrzF might also be physiologically relevant in native strains. E. cloacae strain 99 grows on cyanuric acid with a significant accumulation of biuret.  (+info)

Electroosmotic sampling. Application to determination of ectopeptidase activity in organotypic hippocampal slice cultures. (5/12)

 (+info)

Defining sequence space and reaction products within the cyanuric acid hydrolase (AtzD)/barbiturase protein family. (6/12)

 (+info)

Effects of dextran on five biuret-based procedures for total protein in serum. (7/12)

We evaluated the effect of dextran on values for total protein in serum as measured by the biuret method with five widely used automated instruments: the American Monitor Parallel; the Du Pont aca II; the Roche Cobas-Bio; the Kodak Ektachem 400; and the Beckman Astra 8. Dextran concentrations as great as 25 or 30 g/L had relatively little or no influence on total protein measurements by the latter three instruments. Dextran concentrations exceeding 6 g/L caused falsely low results with the aca, whereas the Parallel gave falsely high results when the dextran concentration exceeded 2 g/L. The aca total protein procedure could be protected from the interference by dextran concentrations up to 30 g/L by injecting 0.4-0.8 mL of ethylene glycol directly into the reagent pack before sampling. However, we could not eliminate the interference with the Parallel procedure by any simple means; we thus recommend that it not be used for measuring total protein in serum samples from patients who are being treated with dextran.  (+info)

Application of a silver-binding assay to the determination of protein in cerebrospinal fluid. (8/12)

We evaluated a silver-binding assay for use in measuring total protein in cerebrospinal fluid. The advantage of this procedure over other methods is that, because of its sensitivity, it requires only a 0.5-microL sample. The procedure, which takes approximately 40 min to complete, involves dilution of 0.5-microL samples to 1 mL with distilled water containing sodium dodecyl sulfate, followed by addition of glutaraldehyde and an ammoniacal silver solution. After color development for 30 min, the reaction is terminated with sodium thiosulfate and the absorbance is measured at 420 nm. This assay displayed within-run and day-to-day precision (CV) of 3.1% to 13% over the range of 210 to 1370 mg/L. It showed substantially less protein-to-protein variation than the Coomassie Blue dye-binding procedure when tested with albumin, globulin, and transferrin. It also yielded an accurate estimation of hemoglobin. Moreover, preliminary studies suggested that it was capable of quantifying immunoglobulin light chains and glycoproteins. In a study of 54 human cerebrospinal fluid samples, results of the silver-binding assay corresponded more closely with those obtained with a rate biuret assay (intraclass correlation coefficient = 0.91) than did either the dye-binding or classical Lowry methods.  (+info)

*Pesticide detection kit

Biuret test (proteins). *Bicinchoninic acid assay (concentration). *Bradford protein assay (concentration). *Lowry protein ...

*Protein adulteration in China

"Feed-grade biuret". Food and Drug Administration. 1 April 2006. Archived from the original on 29 September 2007. Retrieved 6 ... NPN are given to ruminants in the form of pelleted urea, ammonium phosphate and/or biuret. Sometimes slightly polymerized ... For example, Archer Daniels Midland manufactures an NPN supplement for cattle, which contains biuret, triuret, cyanuric acid ...

*Cyanuric acid

"21CFR573.220 Feed-grade biuret". U.S. Food and Drug Administration. April 1, 2006. Retrieved 2007-05-06. "Roughage Buster Plus ... Intermediates in the dehydration include both isocyanic acid, biuret, and triuret: H2N-CO-NH2 → HNCO + NH3 H2N-CO-NH2 + HNCO → ... For example, Archer Daniels Midland manufactures an NPN supplement for cattle, which contains biuret, triuret, cyanuric acid ... C and is suspected also to occur from decomposition of biuret but is produced at a slower rate than that of CYA. Melamine, [C( ...

*Dithiobiuret

Biuret Spofford, W. A., III; Amma, E. L. "Crystal and molecular structure of dithiobiuret" Journal of Crystal and Molecular ...

*Bicinchoninic acid assay

Biuret test Smith, P.K.; et al. (1985). "Measurement of protein using bicinchoninic acid". Anal. Biochem. 150 (1): 76-85. doi: ... Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution ...

*Vikipeedia:Maaülikool/Keelekeskus/Terminitöö - Vikipeedia

Biureedireaktsioon (Biuret test). *Veterinaarne hambaravi (Veterinary dentistry). *Kasside alumiste kuseteede haigus (Feline ...

*Hans Freeman

He received a PhD in 1957 for his work on the structure of biuret hydrate, where he completed most of the calculations for the ... His research group extended its interests beyond purely organic compounds and on to metal complexes (such as copper biuret, one ... Hughes, E. W.; Yakel, H. L.; Freeman, H. C. (1961). "The Crystal Structure of Biuret Hydrate". Acta Crystallogr. 14 (4): 345- ... "Crystallographic Studies of the Biuret Reaction". Nature. 184 (4687): 707-710. Bibcode:1959Natur.184..707F. doi:10.1038/ ...

*Lowry protein assay

Biuret test Bradford protein assay Van Noorden, R.; Maher, B.; Nuzzo, R. (2014). "The top 100 papers". Nature. 514 (7524): 550- ... The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the ...

*Arthur W. Thomas

Thomas, A. W. (1913). A further effort to prepare a colorless biuret reagent. Biochemical Bulletin (New York), 2, 556-8. Thomas ...

*Hugo Schiff

He also worked in the field of amino acids and the Biuret reagent. Born in Frankfurt am Main, Schiff was a student of Friedrich ...

*Copper(II) sulfate

... is also used in the Biuret reagent to test for proteins. Copper sulfate is used to test blood for anemia. ...

*Jonas Kamlet

And he obtained a patent for the use of biuret for animal feed (Kamlet, 1956). Dow Chemicals bought this patent from Kamlet ... synthesis of biuret that could be used as an element of fodder along with hundreds of other patents. His wife, Edna Yadven ...

*Quantitative proteomics

Other more accurate spectrophotometric procedures for protein quantification include the Biuret, Lowry, BCA, and Bradford ...

*Protein primary structure

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ...

*Protein primary structure

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ...

*Triuret

Urea Biuret Cyanuric acid D. Carlström and H. Ringertz "The molecular and crystal structure of triuret" Acta Crystallogr. (1965 ...

*Protein primary structure

Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ...

*Franz Hofmeister

Since biuret has the formula NH2-CO-NH-CO-NH2, that suggested the presence of similar peptide bonds in proteins. primary ... Hofmeister also argued for peptide bonds based on the biuret reaction observed with all proteins but never with free amino ...

*Bradford protein assay

Alternative protein assays include: Ultraviolet-visible spectroscopy Biuret protein assay Lowry protein assay BCA protein assay ...

*Ammeline

... can be synthesized by the pyrolysis of urea or the condensation reaction among 2 moles of dicyandiamide and 1 mole of biuret. 2 ...

*Potassium sodium tartrate

It is a common precipitant in protein crystallography and is also an ingredient in the Biuret reagent which is used to measure ...

*Serum total protein

The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method ...

*Millon's reagent

... and so must be confirmed by other tests for proteins such as the biuret test and the ninhydrin reaction. The reagent is made by ...

*Non-protein nitrogen

... (or NPN) is a term used in animal nutrition to refer collectively to components such as urea, biuret, and ...

*Cyanuric acid amidohydrolase

... biuret + CO2 Thus, the two substrates of this enzyme are cyanuric acid and H2O, whereas its two products are biuret and CO2. ...

*List of organic reactions

Biginelli reaction Bingel reaction Birch reduction Bischler-Möhlau indole synthesis Bischler-Napieralski reaction Biuret test ...
biuret definition: Noun (plural biurets) 1. (organic chemistry) The compound formed by condensation of two molecules of urea with the loss of a molecule of ammonia, (NH2-CO)2NH, or any of its derivatives ...
This intake and digestion study evaluated source of nonprotein nitrogen in cooked molasses blocks supplemented to 18 steers (590 lb) with ad libitum access to prairie hay, Treatments were 1) control (no block), 2) a block containing 60% crude protein with 83% from urea (UREA block), and 3) a block containing 60% crude protein with 42% from urea and 42% from biuret (UREA/BIURET block). Blocks were broken into small pieces to facilitate rapid consumption and fed once daily at .125% of body weight. Forage intake increased by 22%, total intakes (forage plus block) increased by 28%, and digestible organic matter intakes increased by 52% when either UREA or UREA/BIURET blocks were fed. Total diet digestibilities also increased with block supplementation. Intakes and digestibilities were similar for the UREA and UREA/BIURET blocks. Supplementing prairie hay with cooked molasses blocks containing high levels of nonprotein nitrogen increased intake and digestion, but replacing half of the urea with ...
A positive Biuret test shows a pale to intense violet color when there are two or more peptide bonds in a compound. A negative result, or lack of violet color, indicates either a lack of protein or...
Im doing a practical in biology involving the Biuret, Iodine and Benedicts tests. First I need to predict the following, so any help would be useful ...
Five steers (491 ± 21 kg BW) were used in an incomplete 5 × 4 Latin square with four 24-d periods to determine the influence of supplemental non-protein N (NPN) source and supplementation frequency (SF) on nutrient intake and site of digestion in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control and a urea- or biuret-containing supplement placed directly into the rumen daily (D) or every-other-day (2D) at 0700. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. Daily TRT were supplemented CP at 0.04% of BW/d while the 2D TRT were supplemented at 0.08% of BW every-other-day. Therefore, all supplemented TRT received the same quantity of supplemental CP over a 2-d period. Forage OM intake was not affected (P , 0.05) by NPN supplementation, NPN source, or SF. However, total OM and N intake were increased (P , 0.01). wi ...
Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay.. The Biuret assay is a copper ion based protein assay, protein solutions are mixed with an alkaline solution of copper salt, cupric ions (Cu2+). The protein assay is based on the interaction of cupric ions with protein in an alkaline solution. The interaction of cupric ions (Cu2+) with protein results in a purple color that can be read at 545nm. The amount of color produced is proportional to protein ...
Introduction. Chapter 1 ? Chemistry of Life Determining the Presence of Certain Nutrients Kieun Bae, DP2 - Biology HL Introduction The idea of this experiment was to plan the diet for a day and determine if the diet plan contains the necessary nutrients, by testing the food products with chemicals. In this way, we would have better idea about what kind of food product should be included in the daily diet. The meal-plan of the day we have made was following: 1. Breakfast 1. milk 2. cereal 1. Lunch 1. bread 2. juice 3. cheese 4. apple 1. Dinner 1. pasta 2. lattice 3. meat 4. tomato 5. yogurt 6. red wine 7. onion This was planned by discussing the actual food products we commonly take in a day. There are few diary products that are commonly known to contain a lot of protein, necessary carbohydrates for each meal (bread, pasta and cereal) as well as the vegetables that contain many various healthy nutrients including glucose (simple sugar), vitamins, minerals etc. Also, there is meat that is ...
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes. Extinguishing media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam ...
Steam-flaked corn or finely ground corn with different protein levels? What is best to feed high producing dairy cows? Researchers put several corn processing methods to the test.
When filtered alkaline extracts of pulverized bacteria of several varieties are precipitated with acid in the cold, boiled with acid, and all materials thrown down by these procedures removed, there remains a small amount of an alcohol-precipitable material which no longer gives any of the ordinary chemical reactions for proteins, such as the biuret, Hopkins-Cole, Millon, and sulfosalicylic acid reactions. The only protein reaction usually given by this material is a very weak xanthoproteic reaction. Nevertheless, the material, which is, as far as we can determine at present, free from coagulable protein, is specifically precipitable by homologous antiserum and gives specific complement fixation reactions.. Such material can also be obtained from organisms like the influenza bacillus, pneumococcus, and meningococcus by extraction without preliminary grinding of the bacteria, and is present in filtrates of young and old broth cultures of the organisms.. We believe that these acid- and ...
Proteins are extremely important biological molecules, both structurally (e.g. your fingernails are made of the protein keratin, whilst collagen is a very important protein in connective tissue) and functionally as enzymes catalyzing biological reactions. In part A of this prac, students determine the amount of protein in a sample, by effectively measuring the peptide bonds (as each amino acid has a peptide bond connecting it with the next) using a Biuret spectrophotometric assay. Students generate a standard curve, an extremely important tool in biochemistry.. In the second part of the prac, students do a hypothetical experiment on enzymes. Enzymes are protein molecules which act as catalysts of biochemical reactions. Enzymes act by binding the substrate (substance which reacts) and providing a pathway for the reaction, making the conversion from substrate to product faster. Without enzymes, biochemical reactions would take hours or days to proceed. During an enzymatic reaction, substrate is ...
Tratamentul termic al boabelor de soia reduce activitatea inhibitorilor tripsinei si astfel mbunatateste digestibilitatea proteinei. ncalzirea excesiva poate sa distruga sau sa reduca biodisponibilitatea unor aminoacizi termolabili si astfel sa reduca valoarea nutritiva a proteinei din soia. Cercetarile s-au efectuat pe soia toastata si soia netratata termic (granulatie 200 μ), care au fost ncalzite ntr-o etuva cu circulatie fortata a aerului, la temperatura de 120 C, perioade diferite de timp: 5, 10, 15, 20, 25 si 30 minute. S-au determinat indicele de ureaza (UI), solubilitatea proteinei n KOH si indicele de dispersabilitate a proteinei (PDI). Proteina s-a determinat prin metoda biuret. Cercetarile au evidentiat ca indicele de ureaza este util pentru a determina daca faina de soia a fost tratata termic suficient pentru a reduce factorii antinutritionali, dar nu este util pentru determinarea supraprocesarii. Acest test nu este relevant pentru evidentierea ncalzirii excesive, deoarece indicele ...
Looking for online definition of biuret reaction in the Medical Dictionary? biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction mean?
TY - JOUR. T1 - A turbidimetric method for the rapid evaluation of MWF emulsion stability. AU - Deluhery,J.. AU - Rajagopalan,N.. PY - 2005/4/22. Y1 - 2005/4/22. N2 - The stability of metalworking emulsions is a critical characteristic impacting its usefulness as a lubricant and rust inhibitor. Loss of stability usually results in replacement of the fluid leading to both economic loss and environmental discharge. In this paper, the loss of stability caused by accumulation of hardness ions is examined by a turbidimetric method. The method is based on following the change in the wavelength exponent of the emulsions caused by changing particle size. The stability as determined by this method is compared to conventional testing based on phase separation over 24 h. The turbidimetric method is shown to be a more conservative estimate of emulsion stability. The simplicity of the method makes it useful for both product formulation and process control.. AB - The stability of metalworking emulsions is a ...
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This study was aimed at investigating the use of seminal plasma protein concentration to predict fertility and hatchability in the domestic fowl. Forty cocks with acceptable sperm motility and morphology were used and the seminal plasma protein concentration determined by the Biuret method. The result revealed a negative significant correlation between the seminal plasma protein concentration with sperm motility (r = -0.5920, ...
Background: Etiological research in schizophrenia is actively exploring biochemical toxic factor that may be result of an innate metabolic error that factor is thought to be abnormal protein factor. Aim: The present study was undertaken to study the concentration of various protein fractions in acute schizophrenics, to find out the association of serum proteins in acute schizophrenia patients and to find out in what fraction of proteins an abnormality lies. Methods: Study consists of two groups, group one formed by healthy individuals, acting as controls and group two by acute schizophrenia patients. With all aseptic precautions, blood was collected and subjected to electrophoresis and for biuret reaction to find out fractional proteins and total proteins respectively. Analysis was done using Students
EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself.
melamine cyanuric acid and melamine cyanuric acid manufaturers - 113 melamine cyanuric acid manufacturers & melamine cyanuric acid provide quality melamine cyanuric acid from China.
The purpose of this study is to examine and compare the effects of alternate-day reductions in calorie intake or daily calorie restriction on the risk f
Shop IAA-amino acid hydrolase ELISA Kit, Recombinant Protein and IAA-amino acid hydrolase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Iranian Journal of Diabetes and Obesity is scientific quarterly journal published by Shahid Sadoughi University of Medical Sciences
A 30-minute daily or alternate-day facial exercise program sustained over 20 weeks improved the facial appearance of middle-aged women
The continuing thread for those of us following either the 5:2 diet or the alternate-day fasting diet. The 5:2 diet was featured on Horizon in Augus
The continuing thread for those of us following either the 5:2 diet or the alternate-day fasting diet. Both are two versions of Intermittent Fasting,
UK nutritionist Patrick Holford has teamed up with UK fitness expert and former Olympian Kate Staples for his latest book Burn Fat Fast: The Alternate-Day Low-GL Diet Plan.
Looking for online definition of cyanuric acid in the Medical Dictionary? cyanuric acid explanation free. What is cyanuric acid? Meaning of cyanuric acid medical term. What does cyanuric acid mean?
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A filter media component (416, 516, 616) containing activated carbon, which undesirably filters chlorine from water in an aquatic reservoir (301) in which a minimum level of chlorine is desired to be maintained, is inserted into a fluid pumping path for water from the reservoir to reduce levels of cyanuric acid without purging the reservoir. Once levels of cyanuric acid drop below a target reduced concentration, chlorine and other required chemicals may be added to the water to restore proper water balance and increase the chlorine concentration to at least the minimum level of chlorine desired to be maintained. The process, which can be completed in a short period of time with sufficient quantities of activated carbon, need only be performed seasonally or periodically and avoids waste and potential damage associated with purging and replacing the water within the aquatic reservoir to reduce cyanuric acid levels.
Cobalamin (vitamin B12 activity) in milk-based infant formula, turbidimetric method Gaithersburg, MD, AOAC International; AOAC Official Method 986.23 ...
To determine the effect of different types of cobalt supplementation on dairy cow performance and indicators of cobalt status and the benefits of vitamin B12 supplementation in cobalt restricted cows.. Expected Outcomes. ...
Form-A-Feeds blog offers pertinent industry information, company updates, and other news. Read this article about a balanced amino acid diet.
Global Cyanuric Chloride Market Report gives latest insights that includes market sales, share and growth in key regions for their top manufacturers with forecast research for the Cyanuric Chloride Industry.
The next several chapters go into more detail about using fasting for specific benefits: to treat type 2 diabetes, to slow the aging process, to boost heart health.. Part Two is the how-to section of the book. I know, I know: how much how-to information do we need to learn how not eat for awhile? Well, theres more to it than you might think. Theres good advice in here on how to avoid common effects like headaches (salty broths help), who shouldnt fast (kids, pregnant or nursing women, underweight and malnourished people), and various fasting protocols.. Theres no single protocol thats best for everyone. Some people may find they do best with a four-hour eating window, some may prefer alternate-day fasting, some may prefer the 5:2 plan popularized by Dr. Michael Mosley in the U.K, and some may want go to for extended fasts lasting several days or longer. The book explains each protocol and describes the advantages.. My protocol, in case youre curious, is to mix Mosleys 5:2 with 24-hour ...
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Fmoc-D-Cys(Trt)-OHNα-9-Fluorenylmethoxycarbonyl-S-trityl-D-cysteine | Fmoc-D-Cys(Trt)2405 1 g | 75.00 EUR 2405 5 g | 275.00 EUR 2405 ...
Got some in the mail the other day. Was curious whats best way to use. Also , users of NPA or any other high concentration none product
0043] There can be used as the polyisocyanate component A) the conventional aliphatic, cycloaliphatic and in particular aromatic di- and/or poly-isocyanates. Examples of such suitable polyisocyanates are 1,4-butylene diisocyanate, 1,5-pentane diisocyanate, 1,6-hexamethylene diisocyanate (HDI), isophorone diisocyanate (IPDI), 2,2,4- and/or 2,4,4-trimethylhexamethylene diisocyanate, the isomeric bis(4,4-isocyanatocyclohexyl)methanes or mixtures thereof of any desired isomer content, 1,4-cyclohexylene diisocyanate, 1,4-phenylene diisocyanate, 2,4- and/or 2,6-toluene diisocyanate (TDI), 1,5-naphthylene diisocyanate, 2,2- and/or 2,4- and/or 4,4-diphenylmethane diisocyanate (MDI) and/or higher homologues (pMDI), 1,3- and/or 1,4-bis-(2-isocyanato-prop-2-yl)-benzene (TMXDI), 1,3-bis-(isocyanatomethyl)benzene (XDI). In addition to the polyisocyanates mentioned above, modified polyisocyanates with a uretdione, isocyanurate, urethane, carbodiimide, uretonimine, allophanate or biuret structure can also ...
TY - JOUR. T1 - Dentate development in organotypic hippocampal slice cultures from p35 knockout mice. AU - Wenzel, H. Jürgen. AU - Tamse, Catherine T.. AU - Schwartzkroin, Philip A. PY - 2006/12. Y1 - 2006/12. N2 - Abnormal brain development, induced by genetic influences or resulting from a perinatal trauma, has been recognized as a cause of seizure disorders. To understand how and when these structural abnormalities form, and how they are involved in epileptogenesis, it is important to generate and investigate animal models. We have studied one such model, a mouse in which deletion of the p35 gene (p35-/-) gives rise to both structural disorganization and seizure-like function. We now report that aberrant dentate development can be recognized in the organotypic hippocampal slice culture preparation generated from p35-/- mouse pups. In these p35-/- cultures, an abnormally high proportion of dentate granule cells migrates into the hilus and molecular layer, and develops aberrant dendritic and ...
Urea Amidolyase; Contains Both Urea Carboxylase And Allophanate Hydrolase Activities, Degrades Urea To CO2 And NH3; Expression Sensitive To Nitrogen Catabolite Repression And Induced By Allophanate, An Intermediate In Allantoin Degradation; Protein Abundance Increases In Response To DNA Replication Stress
The Physicians Health Study II was a randomized, double-blind, placebo-controlled, factorial trial that began in 1997 and ended in 2011. It was designed to test four supplements -- (1) alternate-day vitamin E (400 international units of synthetic alpha-tocopherol) or its placebo; (2) daily vitamin C (500 mg synthetic ascorbic acid) or its placebo; (3) a daily multivitamin (Centrum Silver) or its placebo; and (4) alternate-day beta carotene (50 mg Lurotin) or its placebo -- in the prevention of cancer, cardiovascular disease, eye disease, and early cognitive decline among 14,641 male physicians aged 50 years or older. Participants were randomly assigned in a 2x2x2x2 factorial trial to receive combinations of the four active supplements or their placebos. Pills and/or packaging were provided by BASF Corporation, Pfizer (formerly Wyeth, American Home Products, and Lederle), and DSM Nutritional Products (formerly Roche Vitamins).. The beta-carotene component was discontinued on March 8, 2003; the ...
sialidase or neuraminidase (EC 3.2.1.18); trans-sialidase (EC 2.4.1.-); anhydrosialidase (EC 4.2.2.15); Kdo hydrolase (EC 3.2.1.-); 2-keto-3-deoxynononic acid hydrolase / KDNase (EC 3.2.1.- ...
I have been learning a lot from reading this site, and am now responsible for pool upkeep (gotten rid of the pool care guy). First, all important test results from my Taylor K-2006: pH: 7.8 FC: 4.0 CC: Absent TA: 80 CH: 230 CYA: Was ~20 on Saturday. Added some cyanuric acid to bring the level up to 30, and will test it again in a day or two.
Ok so I got a free sample a MuscleTechs NaNO Vapor the other day. I figured its free so why not try it. It kind of made me feel like I just downed a
Hello brothers - decided to start a log for future readers who may be considering Androgel as a treatment option. To recap, I have been toying around with going on TRT or not for the last 6 to 8 months or so. I finall…
Post your soldbuchs, maybe tell alittle bit about what you found inside, I got my first soldbuch the other day, something about them fascinates me, It was alot of fun to come home and resear
My dual monitor system has been working fine since new several months ago. The other day, I started experiencing a problem upon startup or reboot: The
I wrote this article the other day in response to a zombie attack from Media Matters, the left-wing commissar-enforcer hypochondriac site. It applies equally
Working people frequently ask retired people what they do to make their days interesting. Well, for example, the other day my wife and I went into town and went.....
Background. For patients with Indian visceral leishmaniasis, amphotericin B deoxycholate is usually given as 15 alternate-day infusions of 1 mg/kg over 30 days (total dose, 15 mg/kg); daily treatment with 1 mg/kg for 20 days (total dose, 20 mg/kg) is also used. This study was done to address the unsettled therapeutic questions of administration schedule (alternate-day vs. daily administration) and dose (1 vs. 0.75 mg/kg) and to determine whether the duration of amphotericin B treatment in Bihar, India, can be shortened to 15 days.. Methods. To compare alternate-day versus daily administration and 1-mg/kg versus 0.75-mg/kg doses and to determine whether the duration of treatment could be abbreviated, Indian subjects randomly received 15 infusions of 1 mg/kg (group A; 245 patients) or 0.75 mg/kg (group B; 244 patients) on alternate days or 1 mg/kg (group C; 500 patients) or 0.75 mg/kg (group D; 496 patients) daily. Noninferiority testing compared 6-month cure rates using a 5% margin.. Results. ...
4I6K: Crystal structure of probable 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE ABAYE1769 (TARGET EFI-505029) from Acinetobacter baumannii with citric acid bound
SDS-PAGE analysis of recombinant VpBD.After electrophoresis, the gel was visualized by Coomassie brilliant blue R250 staining. Lane M: protein molecular standar
Mess Up Your Heart with Intermittent Fasting - posted in Calorie Restriction: As discussed many times before, and contrary to what is often said or implied, there is no specific benefit of alternate-day fasting (ADF)/every-other day feeding (EOD)/intermittent fasting (IF) on lifespan, long-term health, or aging, at the same Calorie intake. Feeding animals once, twice, or several times/day at the same net Calorie intake yields the same lifespan benefit: the limited effect on lifespan boils...
Sowing an earlier crop of maize this spring could help make up for potential shortfalls in winter forage next winter and maintain cow performance at a critical time in the year, according to crop experts.
Nodal TRT reliability of degree and its relationship with nodal degree centrality for F-DOS-based networks.(a) Nodal TRT reliability was mapped in anatomical sp
Couldnt help but post these pics of my little one looking totally 80s the other day. Something about the black tights with big socks (that may as well have been leg warmers) with sneakers was just totally tubular ...
The BH3 interacting-domain death agonist (BID) is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been suggested that BID is involved in the regulation of inflammatory responses in the central nervous system. We found that BID deficiency protected organotypic hippocampal slice cultures in vitro from neuronal injury induced by oxygen-glucose deprivation. In vivo, BID-knockout (KO) mice and WT mice were subjected to 60 minutes of transient middle cerebral artery occlusion to induce focal cerebral ischemia, and allowed to recover for 24 hours. Infarct volumes and functional outcome were assessed and the inflammatory response was evaluated using immunofluorescence, Western blotting, quantitative PCR and Mesoscale multiplex analysis. We observed no difference in the infarct volume or neurological outcome between BID-KO and WT mice. The inflammatory response was reduced by BID deficiency as indicated by a change in microglial/leukocyte
Borna disease virus replication in organotypic hippocampal slice cultures from rats results in selective damage of dentate granule cells. J Virol 79: 11716-23. (#: these authors were co-senior authors) J. Schwenk, N. Harmel, G. Zolles, W. Bildl, A. Kulik, B. Heimrich, O. Chisaka, P. Jonas, U. Schulte, B. Fakler, N. Klöcker (2009 ...
Additional insights into alternative day feeding: Using their wonder-feeder, the scientists also reassessed the effects of alternate day feeding and confirmed the results of a previous study by Anson et al. (2003), who report that "when C57BL/6 mice are maintained on an intermittent fasting (alternate-day fasting) dietary-restriction regimen their overall food intake is not decreased and their body weight is maintained". What is quite surprising, though, is that the mice in Ansons study still saw "beneficial effects that met or exceeded those of caloric restriction including reduced serum glucose and insulin levels and increased resistance of neurons in the brain to excitotoxic stress" - while not all of the corresponding values have been reported in Acosta-Rodriguez et al. (glucose improved significantly with AD, for example), the existing evidence in favor of alternate day fasting, is IMHO sufficient to suggests that it "has beneficial effects on glucose regulation and neuronal resistance to ...
Story ideas from the October 2007 issue of the Journal of Lipid Research include the study of the health effects of a relatively recent diet called alternate-day fasting; how atorvastatin reduces cholesterol and fat in blood vessels; how nutrition affects the breakdown of fats; and a review of intriguing structures on the surface of fat cells called caveolae.
Intermittent fasting: "There is evidence that both alternate-day fasting and periodic fasting may be effective for weight loss, although there are no data that indicate whether the weight loss can be sustained long term. In addition, both eating patterns may be useful for lowering triglyceride concentrations but have little or no effect on total, LDL, or HDL cholesterol concentrations. These protocols may also be beneficial for lowering blood pressure, but a minimum weight loss of 6% may be required to see an effect. Intermittent fasting may also be effective for decreasing fasting insulin and IR, but fasting glucose remains largely unchanged. Future work in this area should aim to examine whether these effects still persist in longer-term (,52 weeks) randomized, controlled trials ...
... - We are specializing in chemicals manufacture and export, our mainly products: trichloroisocyanuric acid, sodium dichloroisocyanurate, cyanuric acid, disinfectant tablet, effervescent chlorine tablet,BCDMH tablet... edie.net
Hi all! Quick question! So, I work on a surgical floor and I am almost at the two year mark. I had an interesting discussion with a colleague the other day about how she never, ever ambulates her
Valentines Day is just never as good as any other day my partner and I spend together, which is part of the reason I have never celebrated it. Each of my partners over the years have asked me, "So what do you want to do for Valentines Day?" and Ive never known what to say. I ask them what they want to do, to turn it on them. If they seem really excited about it, Ill go forth with whatever they want to do. I personally dont care about Valentines Day unless my partner does.. ...
The other day the name of George Carlin came up and I realized it had been more than five years since his death and it was hard to believe it had been
The original SLAT collection contained 48 000 lines in pools of 50. Approximately 12 000 pools were generated and divided up as follows: 48 pools of 50 were assigned to a superpool; a total of 25 superpools were assembled. Nomenclature of the pooled SINS dspm sequences follows the "superpool:pool" pattern such that 01:03 represents Superpool 1: pool 3. The pooled lines were used to create single seeded decent lines from the SLAT collection (24 plants from each pool), this makes genetic analysis much simpler compared with the pools of 50 plants described previously. 24 000 new single seed lines have been generated from the SLAT collection and assigned unique SM numbers.. ...
The free nucleobases and mononucleotides of RNA do not form Watson-Crick base pairs in water, a fact that presents several challenges for the prebiotic synthesis of RNA. 2,6-Diaminopurine and adenosine-5′-monophosphate (AMP) are shown to form supramolecular assemblies with cyanuric acid in water. These assem Prebiotic chemistry and the molecular origins of life
A toxicology research in animals executed following recollects of contaminated pet foods concluded that the combination of melamine and cyanuric acid in diet does result in acute renal failure in cats.[26] A 2008 study generated very similar experimental ends in rats and characterised the melamine and cyanuric acid in contaminated pet foodstuff from the 2007 outbreak.[27] A 2010 research from Lanzhou College attributed renal failure in people to uric acid stone accumulation just after ingestion of melamine resulting in a quick aggregation of metabolites like cyanuric acid diamide (ammeline) and cyanuric acid ...
Looking for nonprotein nitrogen (NPN)? Find out information about nonprotein nitrogen (NPN). gaseous chemical element; symbol N; at. no. 7; interval in which at. wt. ranges 14.00643-14.00728; m.p. −209.86°C;; b.p. −195.8°C;; density 1.25 grams... Explanation of nonprotein nitrogen (NPN)
Borna disease virus replication in organotypic hippocampal slice cultures from rats results in selective damage of dentate granule cells. J Virol 79: 11716-23. (#: these authors were co-senior authors) H. Brinks, S. Conrad, J. Vogt, J. Oldekamp, A. Sierra, L. Deitinghoff, I. Bechmann, G. Alvarez-Bolado, B. Heimrich#, P.P. Monnier#, B.K. Müller#, and T. Skutella# (2004 ...
One of the tests performed at the Department of Biologicals of the Laboratory for Medicins and Medical Devices is an assay for the detection of residual animal serum albumin (bovine serum albumin and ovalbumin) in vaccines. The method used is an immunoprecipitation test using Counter Current Electroforesis (C.C.E.). After electroforesis the precipitate of albumin and anti-albumin serum is stained by Coomassie Brilliant Blue. Traditionally the agarose-gels are visually inspected for the presence of stained precipitates using the light of a bulb shining through opal glass. This report describes the validation of a new detection technique. The agarose-gel is illuminated using two halogen lamps. Under this illumination the precipitates appear as little red lines.Using the traditional detection-method (light bulb) the detection-limit was 1 mul animal serum per litre vaccine. Using the new detection-method (halogen-lamps) the detection-limit is lowered by at least a factor 2. Furthermore, as a ...
CR protects against a number of pathological conditions including diabetes, cancer, heart disease, and neurodegeneration. In PD, an alternate-day feeding schedule where rats consumed 30-40% less calories than ad libitum controls was neuroprotective post-MPTP exposure (Duan and Mattson, 1999). Mice also elicited a neuroprotective response when alternate day feeding begun after exposure to MPTP (Holmer et al., 2005). Primates with a chronic overall 30% reduction in food intake were also resistant to MPTP-induced neurotoxicity (Maswood et al., 2004). These studies prove that CR is beneficial in PD; however, the difficulty to adhere to CR necessitates an alternative method to recapitulate the neuroprotective benefits of CR while bypassing dietary constraints. Evidence from cells treated with serum from CR rats suggests a hormonal factor improves mitochondrial function and cell viability (López-Lluch et al., 2006). We hypothesized that ghrelin may be this hormonal factor, because CR increases plasma ...
Citation: Jacob CC, Reimschuessel R, Von Tungeln LS, Olson GR, Warbritton AR, Hattan DG, Beland FA, Gamboa da Costa G. Dose-response assessment of nephrotoxicity from a 7-day combined exposure to melamine and cyanuric acid in F344 rats. Toxicol Sci. 2011 Feb;119(2):391-7. doi: 10.1093/toxsci/kfq333. Epub 2010 Oct 28.Pubmed Abstract ...
Page 3 - Ok . . . she is a/o and confused at times, and says horrible things to the staff, and is out if control! The other day, I was nothing but nice to her. I took care of her every need, even when she
So the other day I was walking past a lady smoking and she was wearing a Coles uniform and it was literally right next to the shop so she had only just removed herself from the building. I just wasnt sure what to make of it, was it - page 5
A gentleman called the clinic the other day with growing concerns about his wife, and asked us this: “Why does my wife keep falling over?”
Five ruminally cannulated steers (464 ± 26 kg BW) consuming low-quality forage (5% CP; 78% NDF; DM basis) were used in an incomplete 5 × 4 Latin square with four 18-d periods to determine the influence of supplemental N source and supplementation frequency (SF) on ruminal fermentation dynamics. Treatments (TRT), arranged as a 2 × 2 factorial with a negative control (CON), consisted of no supplemental CP and daily (D) or every-other-day (2D) provision of supplements in which SBM or urea was the primary source of supplemental CP. Urea supplements were provided to meet 100% of the degradable intake protein requirement while SBM supplements were provided on an isonitrogenous basis. The D TRT were provided CP at 0.04% of BW/day while 2D TRT were provided CP at 0.08% of BW every-other-day. Therefore, all supplemented TRT received an equal quantity of supplemental CP over a 2-d period. Ruminal indigestible ADF (IADF) passage rate and NH3-N increased (P , 0.04) with supplemental CP on the day all ...
https://bengreenfieldfitness.com/fasting Thousands of books have been written about the latest and greatest diets that will help people lose weight and improve health. But a key element in any successful nutritional health program is a tried-and-true method that most people havent thought about?yet it could be revolutionary for taking health to the next level. This ancient secret is fasting. Fasting is not about starving oneself. When done right, its an incredibly effective therapeutic approach that produces amazing results regardless of diet plan. In fact, Toronto-based nephrologist Dr. Jason Fung has used a variety of fasting protocols with more than 1,000 patients, with fantastic success. Dr. Fung earned his medical degree at the University of Toronto, where he also completed his internal medicine residency before heading to the University of California, Los Angeles, for his fellowship in nephrology. He currently practices as a kidney specialist in Toronto. He is the chief of the department of
By Ronnie Cohen(Reuters Health) - - To his toolbox of Botox, fillers and plastic surgery, cosmetic dermatologist Dr. Murad Alam has added a new, low-cost, noninvasive anti-aging treatment: facial yoga.Dermatologists measured improvements in the appearance of the faces of a small group of middle-age women after they did half an hour of daily face-toning exercises for eight weeks, followed by alternate-day exercises for another 12 weeks.The results surprised lead author Alam, vice chair and professor of dermatology at Northwestern Feinberg School of Medicine in Chicago.
SPE Part: 8B-S049-EBJ Strata® Melamine (55 µm, 80 Å), 100 mg / 3 mL, Tubes , 50/Pk Phase: Proprietary Sorbent Type: Silica-based Format: Tube Target Analytes: Melamine and cyanuric acid
Hello All- a little background I have been married for 1 year and I am in my late 20's. My husband is older by about two decades. He has been on TRT for about 6 or 7 years now. He said when he initially saw a Dr it was b…
idling Badley - My car was not starting the other day and there has been symptoms of the alternator going bad so I did buy a new one and it did make my car...
Look what turned up the other day.... :whoot: :whoot: :whoot: http://farm4.staticflickr.com/3733/11285653116_89b8730fc3_c.jpg Look what turned up in sunny Zimbabwe!!! by pommezimbo, on Flickr Should keep me going for a while :mrgreen: BIG shout out to a certain AWESOME member of this site for making this happen!!!!! You know who you are!!!!! :party: First REAL numbers. (not from strips/ HTH sort of reliable 3 way drop based kit)
A new study found that on the days kids eat pizza, they likely take in more calories, fat and sodium than on other days. On any given day in the United Sta
I started thinking about my About Me page the other day and thought that it might be sort of interesting to come up with some facts about myself to share. Some of these I have written about before, some of them are new information. My hope for this post is that some of you may find out…
Note: Aspirin and aspirin placebo were provided by Bayer HealthCare. Vitamin E and vitamin E placebo were provided by the Natural Source Vitamin E Association, Chicago, Illinois.. Grant Support: By grants HL043851, HL080467, HL099355, and CA047988 from the National Heart, Lung, and Blood Institute and the National Cancer Institute, Bethesda, Maryland.. Potential Conflicts of Interest: Disclosures can be viewed at www.acponline.org/authors/icmje/ConflictOfInterestForms.do?msNum=M12-2816.. Reproducible Research Statement: Study protocol and statistical code: Available from the authors upon written request. Data set: Not available.. Requests for Single Reprints: Nancy R. Cook, ScD, Brigham and Womens Hospital, 900 Commonwealth Avenue East, Boston, MA 02215; e-mail, [email protected] Current Author Addresses: Drs. Cook, Lee, Zhang, Moorthy, and Buring: Brigham and Womens Hospital, 900 Commonwealth Avenue East, Boston, MA 02215.. Author Contributions: Conception and design: N.R. Cook, ...
PP-2B inhibition by thioureylenes resulted in a repression of NFAT-dependent gene transcription and thus might influence long-term apoptotic events by limiting the expression of proapoptotic genes (Holtz-Heppelmann et al., 1998). However, NMDA receptor activation, disruption of calcium homeostasis, and PP-2B activation is most probably associated with rapid cell death initiated by proapoptotic Bad proteins (Wang et al., 1999). Thioureylenes inhibited PP-2B-dependent Bad dephosphorylation and the subsequent initiation of the intrinsic pathway of apoptosis (Yang et al., 1995). Thus, caspase-3 activity, cleavage of the cellular caspase-3 substrate PARP, and caspase-3-dependent cell death were inhibited in SK-N-SH cells and organotypic hippocampal slice cultures. These events depended on active PP-2B activation, as demonstrated by coincubation with CsA, a specific PP-2B inhibitor, and included the repression of procaspase-3 processing.. Our results suggest that thioureylenes also attenuate ...
Early turnout to grass and the use of proven grazing technologies will increase dairy cow performance and reduce costs of milk production. Teagasc has organised a series of on-farm demonstrations of the practicalities of early spring grassland management ...
Early turnout to grass and the use of proven grazing technologies will increase dairy cow performance and reduce costs of milk production. Teagasc has organised a series of on-farm demonstrations of the practicalities of early spring grassland management ...
Description of disease CSF total protein. Treatment CSF total protein. Symptoms and causes CSF total protein Prophylaxis CSF total protein
It was ignored for a year until I removed it from its pot the other day. The roots were largely rotten so I removed all but the sound material and the divided it into three. A few green shoots are just about visible at the top. ...
Toni Mullany of Waynesville sent me an email the other day noting a guest scoping out her phlox. She described the critter thusly:
If how much tylenol can a 110 pound person take it is the reduction of various oral hypoglycaemic drugs of morbidity in late inspiratory crackles And. Com- binations carries a semi-synthetic taxane derivative of hepatic first-pass metabolism It has vT The newer progestogens. Substances such as anxiety is used how much to sell 5 mg hydrocodone to antibiotics will continue on pH above 7 673. Treatment is a balance adequate but is generally, which was no evidence of insulin, e. Insulin is at his or added t4 somatostatin, a lower-affinity/higher- capacity. It is a proton-pump inhibitors, oral prodrug which emphasize the elderly The hypotension. The mean corpuscular volume of pitting and blood pressure. CGMP previously useful in nSTEMI following recovery from the neurological event. The majority of coronary events, and goserelin given intravenously Alternate-day dosing should be primary. Therefore how long does it take for a viagra to work pregnant women who are not enter the sensation of urine. ...
Thanks Chem Geek for your reply. I also asked this question in other forums and got different answers. Local pool supply shops suggested to not use a chlorine based shock because of the generation of unwanted chemistry in a bromine pool (dont remember now what that was supposed to be). In any case, I went with a non-chlorine shocker. I understand that with the DCDMH that shocking is not as necessary but I did use shocker with all the fresh water. In any case, the bromine testing seems to be good. The stabilizer, cyanuric acid, I understand to be required for chlorine in outside pools to protect from breakdown due to UV light. In any case, my understanding was that mono-chloroamine was the cause of chlorine smell. My pool is indoor and is directly attached to the basement floor in a walkout configuration. Still there was some cyanuric acid in the pool even though it should not be required. In any case, this configuration is very sensitive to the chemical smell. I have now moved on to a different ...
Buy a 1975 Nissan 620 Oil Filter at discount prices. Choose top quality brands AC Delco, Beck Arnley, Bosch, Fram, Hastings, K&N, Motorcraft, NPN, Pronto, WIX.
2SC1473A Japanese silicon NPN triple diffusion planer type transistor for general amplification. |br||br|Features:|br|High collector to emitter voltage VCEO|br|High transition frequency fT|br||br|TO-92 package.|br|G15013
The enzyme, characterized from the bacterium Pseudomonas sp. ADP, participates in the degradation of cyanuric acid, an intermediate in the degradation of s-triazine herbicides such as atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine]. The enzyme is a heterotetramer composed of a catalytic subunit (AtzE) and an accessory subunit (AtzG) that stabilizes the complex ...

Biuret reaction | definition of biuret reaction by Medical dictionaryBiuret reaction | definition of biuret reaction by Medical dictionary

... biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction ... Looking for online definition of biuret reaction in the Medical Dictionary? ... biuret test either of the tests done with biuret.. bi·u·ret re·ac·tion. the formation of biuret that gives a violet color as a ... biuret reaction. Also found in: Dictionary, Encyclopedia. biuret. [bi´u-ret] a urea derivative; its presence is detected after ...
more infohttps://medical-dictionary.thefreedictionary.com/biuret+reaction

Biuret | Definition of Biuret at Dictionary.comBiuret | Definition of Biuret at Dictionary.com

Biuret definition, a white crystalline substance, C2H5O2N3⋅H2O, soluble in water and alcohol, used for the identification of ... biuret. bituminous, bituminous coal, bitzer, biunique, biuniqueness, biuret, biuret reaction, bivalence, bivalent, bivalent ... biuret. *. The absence of a biuret reaction would point in that direction and the question must await further study. ... On now testing a portion of the clear filtrate for peptone by the biuret test, not a trace of a reaction could be obtained. ...
more infohttps://www.dictionary.com/browse/biuret

SPECTRUM Biuret, 500g - 26YC09|B0513-500G - GraingerSPECTRUM Biuret, 500g - 26YC09|B0513-500G - Grainger

Looking for SPECTRUM Biuret, 500g (26YC09)? Graingers got your back. Price:$649.00. Easy ordering & convenient delivery. Log- ...
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Biuret dictionary definition | biuret definedBiuret dictionary definition | biuret defined

... plural biurets) 1. (organic chemistry) The compound formed by condensation of two molecules of urea with the loss of a molecule ... biuret. Noun (plural biurets). *(organic chemistry) The compound formed by condensation of two molecules of urea with the loss ... Biuret (allophanamide), NH 2 CO NH CO NH 2, is formed by heating urea; by the action of ammonia on allophanic ester; and by ... How would you define biuret? Add your definition here.. Please enable JavaScript to view the comments powered by Disqus.. ...
more infohttp://www.yourdictionary.com/biuret

Biuret - WikipediaBiuret - Wikipedia

The biuret test is a chemical test for proteins and polypeptides. It is based on the biuret reagent, a blue solution that turns ... The term "biuret" also describes a family of organic compounds with the functional group -(HN-CO-)2N-. Thus dimethyl biuret is ... For example, the trimer of 1,6-hexamethylene diisocyanate is also known as HDI-biuret. Biuret is also used as a non-protein ... The test and reagent do not actually contain biuret; they are so named because both biuret and proteins have the same response ...
more infohttps://en.wikipedia.org/wiki/Biuret

What Color Is a Positive Biuret Solution Test? | Reference.comWhat Color Is a Positive Biuret Solution Test? | Reference.com

A positive Biuret test shows a pale to intense violet color when there are two or more peptide bonds in a compound. A negative ... A positive Biuret test shows a pale to intense violet color when there are two or more peptide bonds in a compound. A negative ... The Biuret test does not reveal protein specificity, it simply detects the presence of two carbonyl groups attached to a ...
more infohttps://www.reference.com/health/color-positive-biuret-solution-test-652723bf35016c52

Biuret amidohydrolase - WikipediaBiuret amidohydrolase - Wikipedia

In enzymology, a biuret amidohydrolase (EC 3.5.1.84) is an enzyme that catalyzes the chemical reaction biuret + H2O ⇌ {\ ... The systematic name of this enzyme class is biuret amidohydrolase. This enzyme participates in atrazine degradation. Cook AM, ... displaystyle \rightleftharpoons } urea + CO2 + NH3 Thus, the two substrates of this enzyme are biuret and H2O, whereas its 3 ...
more infohttps://en.wikipedia.org/wiki/Biuret_amidohydrolase

Biuret Test for Proteins - Stock Image - C039/1301 - Science Photo LibraryBiuret Test for Proteins - Stock Image - C039/1301 - Science Photo Library

The Biuret reagent contains copper(II) sulfate (CuSO4) and sodium hydroxide (NaOH), with potassium sodium tartrate (KNaC4H4O6) ... Biuret test for proteins. The Biuret reagent contains copper(II) sulfate (CuSO4) and sodium hydroxide (NaOH), with potassium ... Here blue-coloured Biuret reagent was added to a solution of albumin (egg white) in a test tube. The solution turned violet ( ...
more infohttps://www.sciencephoto.com/media/931156/view/biuret-test-for-proteins

Biology-Online • View topic - Biuret, Iodine, Benedicts testBiology-Online • View topic - Biuret, Iodine, Benedict's test

Biuret test. Does the following contain proteins? If so, what would the relative concentration be? (1ml sample size). Egg ... Biuret, Iodine, Benedicts test. Debate and discussion of any biological questions not pertaining to a particular topic. ... Biuret, Iodine, Benedicts test. by Zel » Sat Mar 19, 2011 8:51 am ... Im doing a practical in biology involving the Biuret, Iodine and Benedicts tests. First I need to predict the following, so ...
more infohttp://www.biology-online.org/biology-forum/about21001.html?hilit=Iodine%20test

Question about Biuret reagent lab - Biology Forum | Biology-Online Dictionary, Blog & ForumQuestion about Biuret reagent lab - Biology Forum | Biology-Online Dictionary, Blog & Forum

Question about Biuret reagent lab. Discussion of all aspects of biological molecules, biochemical processes and laboratory ... We recently completed a lab dealing with Biuret reagent and testing it on a protein (test) and distilled water (control). We ...
more infohttps://www.biology-online.org/biology-forum/viewtopic.php?t=3091

β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret) | Sigma-Aldrichβ-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret) | Sigma-Aldrich

"β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret); CAS Number: 9001- ... aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret) Synonym: β-. D. -Glucuronide glucuronosohydrolase ...
more infohttps://www.sigmaaldrich.com/catalog/product/sigma/g8162?lang=en®ion=US

Protein Test - Biuret Reagent Solution, 4oz. for sale. Buy from The Science Company.Protein Test - Biuret Reagent Solution, 4oz. for sale. Buy from The Science Company.

The characteristic color of a positive biuret test.. By Ozone aurora/Philip Evans - Own work, CC BY-SA 3.0, Link Protein Test ... Biuret Reagent is an aqueous solution of potassium sodium tartrate treated with cupric sulfate and sodium hydroxide. In the ... Add 3 drops of Biuret reagent solution to each test tube. Shake gently to mix. ... Biuret solution. *To a test tube, add 40 drops of liquid to be tested. ...
more infohttps://www.sciencecompany.com/Biuret-Reagent-Solution-125mL-4-oz-P6410.aspx

Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage:  Daily and Alternate Day Supplementation | EOARC -...Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage: Daily and Alternate Day Supplementation | EOARC -...

Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage: Daily and Alternate Day Supplementation. Site of ... In addition, duodenal bacterial N flow was increased with CP supplementation (P = 0.04) and for biuret compared with urea (P , ... These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality ... Daily and alternate-day supplementation of urea or biuret to ruminnts consuming low-quality forage: II Effects on site of ...
more infohttp://oregonstate.edu/dept/eoarc/past-research/dexperiment2

Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and...Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and...

1. Biuret test Biuret test determines the presence of protein by showing the color purple. The results were following: § Table ... The chemical tests that are used for the experiments are: Benedict test, Biuret test and Iodine test, which test for glucose, ... Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and ... www.scumdoctor.com/nutrition/protein/Biuret-Test-For-Proteins.html - 21/10/10 [5] ?Iodine Test? by Rimlee Bhuyan - 7/23/2010 ? ...
more infohttp://www.markedbyteachers.com/international-baccalaureate/group-4-projects/determine-the-presence-of-reducing-sugar-protein-and-starch-in-the-food-products-by-using-benedict-test-biuret-test-and-iodine-test.html

Biuret | definition of biuret by Medical dictionaryBiuret | definition of biuret by Medical dictionary

... biuret explanation free. What is biuret? Meaning of biuret medical term. What does biuret mean? ... Looking for online definition of biuret in the Medical Dictionary? ... Related to biuret: Biuret test, Biuret reagent. biuret. [bi´u-ret] a urea derivative; its presence is detected after addition ... biuret test either of the tests done with biuret.. bi·u·ret. (bī-yū-ret), A derivative of urea obtained by heating, ...
more infohttps://medical-dictionary.thefreedictionary.com/biuret

Cyanuric acid biodegradation via biuret: Physiology, taxonomy, and geospatial distribution<...Cyanuric acid biodegradation via biuret: Physiology, taxonomy, and geospatial distribution<...

The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ...
more infohttps://experts.umn.edu/en/publications/cyanuric-acid-biodegradation-via-biuret-physiology-taxonomy-and-g

BiuretBiuret

Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes. Extinguishing media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam ...
more infohttp://www.chemicaldictionary.org/dic/B/Biuret_1377.html

What is the reaction of K + H2O? | Reference.comWhat is the reaction of K + H2O? | Reference.com

What is a biuret solution?. A: A biuret solution is a liquid mixture of potassium hydroxide and copper sulfate used to detect ...
more infohttps://www.reference.com/science/reaction-k-h2o-faa458d94083b492

Sensors  | Free Full-Text | Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as...Sensors | Free Full-Text | Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as...

The measurement was done as follows: 180 μl of the biuret solution was mixed with 45 μl of real or standard sample; after ... For determination of the total protein content the biuret solution (15 mM potassium sodium tartrate, 100 mM NaI, 15 mM KI and 5 ... At the plants treated with silver(I) ions the changes were observable also in total content of proteins determined using biuret ... 2.4.2 Protein determination - Biuret test. Weighed plant tissues (approximately 0.2 g) were transferred to a test-tube. Then, ...
more infohttp://www.mdpi.com/1424-8220/8/1/445/htm

Indicators and Dyes for Chemical Processing - Alfa AesarIndicators and Dyes for Chemical Processing - Alfa Aesar

View our list of indicators and dyes used to determine pH or presence of metal ions. Purchase indicators and dyes at Alfa Aesar online.
more infohttps://www.alfa.com/en/indicators-and-dyes/

IUCr) Acta Crystallographica Volume 20, Part 2, February 1966IUCr) Acta Crystallographica Volume 20, Part 2, February 1966

Crystallographic studies of the biuret reaction. II. Structure of bis-biuret-copper(II) dichloride, Cu(NH2CONHCONH2)2C12. ...
more infohttp://journals.iucr.org/q/issues/1966/02/00/

Foliar applications of Lo-Biuret Urea and Potassium Phosphite to Navel Orange treesFoliar applications of Lo-Biuret Urea and Potassium Phosphite to Navel Orange trees

Treatments included: normal grower practice, winter low biuret (LB) urea application, summer LB urea application, winter LB ... Foliar applications of Lo-Biuret Urea and Potassium Phosphite to Navel Orange trees. ...
more infohttps://repository.arizona.edu/handle/10150/198099

Reagents - Products, news, whitepaper, companies, specialist informationReagents - Products, news, whitepaper, companies, specialist information

Biuret reagent Biuret reagent The Biuret reagent is made of potassium hydroxide (KOH) and copper (II) sulfate (CuSO4), together ...
more infohttps://www.bionity.com/en/reagents.html

Pesticide detection kit - WikipediaPesticide detection kit - Wikipedia

Biuret test (proteins). *Bicinchoninic acid assay (concentration). *Bradford protein assay (concentration). *Lowry protein ...
more infohttps://en.wikipedia.org/wiki/Pesticide_detection_kit
  • Thus single AMINO ACIDS and DIPEPTIDES with only one peptide bond will not give a positive Biuret reaction. (thefreedictionary.com)
  • The biuret reaction of amino acids and small peptides was completed by the time of the first postbaseline reading: within ~2-4 s depending on the speed of mixing. (thefreedictionary.com)
  • The biuret reaction commonly has been considered to selectively measure peptides 3 amino acid residues in length or longer (3), although there have been reports extending back many years indicating that amino acids, dipeptides, and other compounds are reactive (30-33). (thefreedictionary.com)
  • In general, organic biurets (those with alkyl or aryl groups in place of one or more H atoms) are prepared by trimerization of isocyanates. (wikipedia.org)
  • The protein concentration of the myofibril solution was determined by the biuret method (Gornall, Bardawill, & Maxima, 1949). (thefreedictionary.com)
  • What is a biuret solution? (reference.com)
  • Determine the concentration of an unknown protein solution using two different spectroscopic methods (the biuret method and UV method) for the measurement of protein concentration. (rushtermpapers.com)
  • In this practical, you will determine the concentration of an unknown protein solution using two different spectroscopic methods (the biuret method and UV method) for the measurement of protein concentration. (rushtermpapers.com)
  • The purple solution comes from the reduction of Cu in the Biuret solution. (majortests.com)
  • Biuret is also used as a non-protein nitrogen source in ruminant feed, where it is converted into protein by gut microorganisms. (wikipedia.org)
  • Methods for the determination of total urinary protein that use precipitation of protein and indirect biuret are accurate, but they also are time-consuming and, therefore, not widely used. (thefreedictionary.com)
  • To validate further the modified dilution protocol we compared the pyrogallol red and Vitros methods to a manual biuret reference procedure. (thefreedictionary.com)
  • Here, we show the biuret pathway was more than 1 order of magnitude more prevalent than the original Pseudomonas sp. (umn.edu)