Biuret
Biuret Reaction
Allophanate Hydrolase
Triazines
Colorimetry
Enterobacter cloacae
Chemistry, Organic
Dictionaries as Topic
Organic Chemistry Phenomena
Flow Injection Analysis
Glass
Rumen
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Dietary Supplements
Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.
Poaceae
Anomura
Yogurt
Bread
Cheese
Respiratory Protective Devices
Skating
Protective Clothing
National Institute for Occupational Safety and Health (U.S.)
Hydrogen
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Potassium
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
Gases
The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)
Molluscum Contagiosum
Anemia, Iron-Deficiency
Iron
Iron, Dietary
Anemia, Hypochromic
Anemia characterized by a decrease in the ratio of the weight of hemoglobin to the volume of the erythrocyte, i.e., the mean corpuscular hemoglobin concentration is less than normal. The individual cells contain less hemoglobin than they could have under optimal conditions. Hypochromic anemia may be caused by iron deficiency from a low iron intake, diminished iron absorption, or excessive iron loss. It can also be caused by infections or other diseases, therapeutic drugs, lead poisoning, and other conditions. (Stedman, 25th ed; from Miale, Laboratory Medicine: Hematology, 6th ed, p393)
Ferritins
Iron-containing proteins that are widely distributed in animals, plants, and microorganisms. Their major function is to store IRON in a nontoxic bioavailable form. Each ferritin molecule consists of ferric iron in a hollow protein shell (APOFERRITINS) made of 24 subunits of various sequences depending on the species and tissue types.
Hemoglobins
Crystallography, X-Ray
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Models, Molecular
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Binding Sites
European Union
The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)
Daily and alternate day supplementation of urea or biuret to ruminants consuming low-quality forage: I. Effects on cow performance and the efficiency of nitrogen use in wethers. (1/12)
Two experiments were conducted to determine the influence of supplemental nonprotein N (NPN) provided daily (D) or every other day (2D) on ruminant performance and N efficiency. Treatments included an unsupplemented control (CON) and a urea (28.7% CP) or biuret (28.6% CP) supplement provided D or 2D at 0700. In Exp. 1, five wethers (39 +/- 1 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental NPN source and supplementation frequency (SF) on the efficiency of N use in lambs consuming low-quality grass straw (4% CP). The amount of CP supplied by each supplement was approximately 0.10% of BW/d (averaged over a 2-d period). In Exp. 2, 80 Angus x Hereford cows (540 +/- 8 kg BW) in the last third of gestation were used to determine the effect of NPN source and SF on cow performance. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. The supplemented treatments received the same amount of supplemental N over a 2-d period; therefore, the 2D treatments received double the quantity of supplemental N on their respective supplementation day than the D treatments. In Exp. 1, total DM, OM, and N intake; DM, OM, and N digestibility; N balance; and digested N retained were greater (P < 0.03) for supplemented than for CON wethers, with no difference (P > 0.05) between NPN sources or SF. Plasma urea-N (PUN) was increased with N supplementation compared with CON (P < 0.01), and urea treatments had greater PUN than biuret (P < 0.01). In addition, PUN was greater (P = 0.02) for D than for 2D treatments. In Exp. 2, pre- and postcalving (within 14 d and 24 h after calving, respectively) cow weight and body condition score change were more positive (P < 0.05) for supplemented groups than for CON. These results suggest that supplements containing urea or biuret as the primary source of supplemental N can be effectively used by lambs and cows consuming low-quality forage, even when provided every other day. (+info)Daily and alternate-day supplementation of urea or biuret to ruminants consuming low-quality forage: II. Effects on site of digestion and microbial efficiency in steers. (2/12)
Five steers (491 +/- 21 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental non-protein N (NPN) source and supplementation frequency (SF) on nutrient intake and site of digestion in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control and a urea- or biuret-containing supplement placed directly into the rumen daily (D) or every other day (2D) at 0700. The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement. Daily TRT were supplemented CP at 0.04% of BW/d, whereas the 2D TRT were supplemented at 0.08% of BW every other day. Therefore, all supplemented TRT received the same quantity of supplemental CP over a 2-d period. Forage OM intake was not affected (P > 0.05) by NPN supplementation, NPN source, or SF; however, total OM and N intake were increased (P < 0.01) with CP supplementation. Duodenal flow of N was greater (P = 0.04) with CP supplementation compared with the control. In addition, duodenal bacterial N flow was increased with CP supplementation (P = 0.04) and for biuret compared with urea (P < 0.01). Bacterial efficiency (g bacterial N/kg OM truly digested in the rumen) was greater (P = 0.05) for biuret than for urea. Apparent total-tract N digestibility was increased with NPN supplementation (P < 0.01) but not affected by NPN source or SF. These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality forage. (+info)Daily and alternate-day supplementation of urea or biuret to ruminants consuming low-quality forage: III. Effects on ruminal fermentation characteristics in steers. (3/12)
Five ruminally and duodenally cannulated steers (491 +/- 21 kg BW) were used in an incomplete 5 x 4 Latin square with four 24-d periods to determine the influence of supplemental nonprotein N (NPN) source and supplementation frequency (SF) on the dynamics of ruminal fermentation in steers consuming low-quality grass straw (4% CP). Treatments (TRT) included an unsupplemented control (CON) and a urea or biuret supplement that were placed directly into the rumen at 0700 daily (D) or every other day (2D). The NPN treatments were formulated to provide 90% of the estimated degradable intake protein requirement; therefore, the urea and biuret treatments received the same amount of supplemental N over a 2-d period. Daily TRT were supplemented with CP at 0.04% of BW/d, whereas the 2D TRT were supplemented at 0.08% of BW every other day. Forage was provided at 120% of the previous 5-d average intake in two equal portions at 0715 and 1900. Ruminal fluid was collected 0, 3, 6, 9, 12, and 24 h after supplementation on a day of and a day before supplementation for all TRT. Ruminal NH3-N increased (P < 0.04) with CP supplementation on the day all supplements were provided and on the day on which only daily supplements were provided compared with the CON. However, an NPN source x SF interaction (P = 0.03) on the day all supplements were provided indicated that NH3-N increased at a greater rate for urea as SF decreased compared with biuret. Ruminal NH3-N on the day only daily supplements were provided was greater (P = 0.02) for D compared with 2D. On the day all supplements were provided, D increased (P = 0.05) ruminal indigestible acid detergent fiber passage rate and ruminal fluid volume compared with 2D. These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality forage without adversely affecting ruminal fermentation, even when provided every other day. (+info)Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth. (4/12)
TrzF, the allophanate hydrolase from Enterobacter cloacae strain 99, was cloned, overexpressed in the presence of a chaperone protein, and purified to homogeneity. Native TrzF had a subunit molecular weight of 65,401 and a subunit stoichiometry of alpha(2) and did not contain significant levels of metals. TrzF showed time-dependent inhibition by phenyl phosphorodiamidate and is a member of the amidase signature protein family. TrzF was highly active in the hydrolysis of allophanate but was not active with urea, despite having been previously considered a urea amidolyase. TrzF showed lower activity with malonamate, malonamide, and biuret. The allophanate hydrolase from Pseudomonas sp. strain ADP, AtzF, was also shown to hydrolyze biuret slowly. Since biuret and allophanate are consecutive metabolites in cyanuric acid metabolism, the low level of biuret hydrolase activity can have physiological significance. A recombinant Escherichia coli strain containing atzD, encoding cyanuric acid hydrolase that produces biuret, and atzF grew slowly on cyanuric acid as a source of nitrogen. The amount of growth produced was consistent with the liberation of 3 mol of ammonia from cyanuric acid. In vitro, TrzF was shown to hydrolyze biuret to liberate 3 mol of ammonia. The biuret hydrolyzing activity of TrzF might also be physiologically relevant in native strains. E. cloacae strain 99 grows on cyanuric acid with a significant accumulation of biuret. (+info)Electroosmotic sampling. Application to determination of ectopeptidase activity in organotypic hippocampal slice cultures. (5/12)
(+info)Defining sequence space and reaction products within the cyanuric acid hydrolase (AtzD)/barbiturase protein family. (6/12)
(+info)Effects of dextran on five biuret-based procedures for total protein in serum. (7/12)
We evaluated the effect of dextran on values for total protein in serum as measured by the biuret method with five widely used automated instruments: the American Monitor Parallel; the Du Pont aca II; the Roche Cobas-Bio; the Kodak Ektachem 400; and the Beckman Astra 8. Dextran concentrations as great as 25 or 30 g/L had relatively little or no influence on total protein measurements by the latter three instruments. Dextran concentrations exceeding 6 g/L caused falsely low results with the aca, whereas the Parallel gave falsely high results when the dextran concentration exceeded 2 g/L. The aca total protein procedure could be protected from the interference by dextran concentrations up to 30 g/L by injecting 0.4-0.8 mL of ethylene glycol directly into the reagent pack before sampling. However, we could not eliminate the interference with the Parallel procedure by any simple means; we thus recommend that it not be used for measuring total protein in serum samples from patients who are being treated with dextran. (+info)Application of a silver-binding assay to the determination of protein in cerebrospinal fluid. (8/12)
We evaluated a silver-binding assay for use in measuring total protein in cerebrospinal fluid. The advantage of this procedure over other methods is that, because of its sensitivity, it requires only a 0.5-microL sample. The procedure, which takes approximately 40 min to complete, involves dilution of 0.5-microL samples to 1 mL with distilled water containing sodium dodecyl sulfate, followed by addition of glutaraldehyde and an ammoniacal silver solution. After color development for 30 min, the reaction is terminated with sodium thiosulfate and the absorbance is measured at 420 nm. This assay displayed within-run and day-to-day precision (CV) of 3.1% to 13% over the range of 210 to 1370 mg/L. It showed substantially less protein-to-protein variation than the Coomassie Blue dye-binding procedure when tested with albumin, globulin, and transferrin. It also yielded an accurate estimation of hemoglobin. Moreover, preliminary studies suggested that it was capable of quantifying immunoglobulin light chains and glycoproteins. In a study of 54 human cerebrospinal fluid samples, results of the silver-binding assay corresponded more closely with those obtained with a rate biuret assay (intraclass correlation coefficient = 0.91) than did either the dye-binding or classical Lowry methods. (+info)
Biuret test for protein lab report pdf
Biuret dictionary definition | biuret defined
Effects of nonprotein nitrogen source in blocks on intake and digestion of prairie hay by steers
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Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage: Daily and Alternate Day Supplementation | EOARC -...
Assays for Protein Quantification
Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and...
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Biuret reaction | definition of biuret reaction by Medical dictionary
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"Feed-grade biuret". Food and Drug Administration. 1 April 2006. Archived from the original on 29 September 2007. Retrieved 6 ... NPN are given to ruminants in the form of pelleted urea, ammonium phosphate and/or biuret. Sometimes slightly polymerized ... For example, Archer Daniels Midland manufactures an NPN supplement for cattle, which contains biuret, triuret, cyanuric acid ...
Dithiobiuret
Biuret Spofford, W. A.; Amma, E. L. (1972). "Crystal and molecular structure of dithiobiuret". Journal of Crystal and Molecular ...
Fertilizer
Mikkelsen, R.L. (2007). "Biuret in Urea Fertilizers" (PDF). Better Crops. 91 (3): 6-7. Archived from the original (PDF) on 22 ...
Aayon Ibn Aayon
Biuret, Lebanon: دار الكتب العلمية. p. 310. Archived from the original on 24 September 2020. Mahfouz, Muhammad (1982). تراجم ... Ibn Abi Usaibia (1995). عيون الأنباء في طبقات الأطباء (in Arabic) (first ed.). Biuret, Lebanon: دار مكتبة الحياة. p. 564. ... المؤلفين التونسيين (in Arabic). first (first ed.). Biuret, Lebanon: دار الغرب الإسلامي. p. 52. Archived from the original on 24 ...
Bicinchoninic acid assay
Biuret test Smith, P.K.; et al. (1985). "Measurement of protein using bicinchoninic acid". Anal. Biochem. 150 (1): 76-85. doi: ... Bradford protein assay or biuret reagent. The total protein concentration is exhibited by a color change of the sample solution ...
Boby Techi
Prior to winning the K-Pop India Contest, Techi toured with the Korean pop-rock band BIURET on their maiden visit to India. ... CS1 maint: discouraged parameter (link) "'BIURET' Tour". The Pioneer. 1 November 2013. Retrieved 16 November 2013. K-Pop ...
Hexamethylene diisocyanate
HDI is also sold oligomerized as the trimer or biuret. Although more viscous in these forms, it reduces the volatility and ...
Allophanic acid
Biuret can be viewed as the amide of allophanic acid. The compound can be prepared by treating urea with sodium bicarbonate: ...
Triuret
Urea Biuret Cyanuric acid International Union of Pure and Applied Chemistry (2014). Nomenclature of Organic Chemistry: IUPAC ...
Jonas Kamlet
And he obtained a patent for the use of biuret for animal feed (Kamlet, 1956). Dow Chemicals bought this patent from Kamlet ... synthesis of biuret that could be used as an element of fodder along with hundreds of other patents. His wife, Edna Yadven ...
Quantitative proteomics
Other more accurate spectrophotometric procedures for protein quantification include the Biuret, Lowry, BCA, and Bradford ...
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Biureedireaktsioon (Biuret test). *Veterinaarne hambaravi (Veterinary dentistry). *Kasside alumiste kuseteede haigus (Feline ...
Protein methods
Lowry assay is similar to biuret assays, but it uses Folin reagent which is more accurate for quantification. Folin reagent is ...
Millon's reagent
Therefore, the biuret test or the ninhydrin reaction are used along with it to confirm the presence of proteins. Benedict's ...
Franz Hofmeister
Since biuret has the formula NH2-CO-NH-CO-NH2, that suggested the presence of similar peptide bonds in proteins. primary ... Hofmeister also argued for peptide bonds based on the biuret reaction observed with all proteins but never with free amino ...
Bradford protein assay
Alternative protein assays include: Ultraviolet-visible spectroscopy Biuret protein assay Lowry protein assay BCA protein assay ...
Ammeline
... can be synthesized by the pyrolysis of urea or the condensation reaction among 2 moles of dicyandiamide and 1 mole of biuret. 2 ...
Potassium sodium tartrate
It is a common precipitant in protein crystallography and is also an ingredient in the Biuret reagent which is used to measure ...
Protein primary structure
Franz Hofmeister made the proposal in the morning, based on his observations of the biuret reaction in proteins. Hofmeister was ...
Cyanuric acid amidohydrolase
... biuret + CO2 Thus, the two substrates of this enzyme are cyanuric acid and H2O, whereas its two products are biuret and CO2. ...
Chemical test
... sugars Rapid furfural test to distinguish between glucose and fructose The Bicinchoninic acid assay tests for proteins Biuret ...
Ureas
... using primary amines must be carefully controlled as the isocyanate intermediate can react with the urea to form a biuret: R2NC ...
Isocyanate
The addition of an isocyanate to a urea gives a biuret: R2NC(O)N(H)R' + R"NCO → R2NC(O)NR'C(O)NHR" Reaction between a di- ...
List of EC numbers (EC 3)
... biuret amidohydrolase EC 3.5.1.85: (S)-N-acetyl-1-phenylethylamine hydrolase EC 3.5.1.86: mandelamide amidase EC 3.5.1.87: N- ...
Bicinchoninic acid
... is used to analyze to monitor cuprous ion presence due to its purple coloration via a biuret reactionIn this ...
Biuret
The biuret test is a chemical test for proteins and polypeptides. It is based on the biuret reagent, a blue solution that turns ... The term "biuret" also describes a family of organic compounds with the functional group -(HN-CO-)2N-. Thus, dimethyl biuret is ... The test and reagent do not actually contain biuret; they are so named because both biuret and proteins have the same response ... This notice reports that biuret reacts with alkaline copper sulfate to produce a red solution -- the so-called "Biuret test" ...
Biuret amidohydrolase
In enzymology, a biuret amidohydrolase (EC 3.5.1.84) is an enzyme that catalyzes the chemical reaction biuret + H2O ⇌ {\ ... The systematic name of this enzyme class is biuret amidohydrolase. This enzyme participates in atrazine degradation. Cook AM, ... displaystyle \rightleftharpoons } urea(CH4N2O) + CO2 + NH3 Thus, the two substrates of this enzyme are biuret and H2O, whereas ...
Biuret test
In Poland, the biuret test is also known as the Piotrowski test, in honor of the Polish physiologist, Gustaw Piotrowski (b. ... The biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per ... The biuret (IPA: /ˌbaɪjəˈrɛt/, /ˈbaɪjəˌrɛt/) test, also known as Piotrowski's test, is a chemical test used for detecting the ... Despite its name, the reagent does not in fact contain biuret ((H2N-CO-)2NH). The test is named so because it also gives a ...
List of organic reactions
Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia Foundation, Inc., a non-profit organization ...
Biuret - Wikipedia
The biuret test is a chemical test for proteins and polypeptides. It is based on the biuret reagent, a blue solution that turns ... The term "biuret" also describes a family of organic compounds with the functional group -(HN-CO-)2N-. Thus, dimethyl biuret is ... The test and reagent do not actually contain biuret; they are so named because both biuret and proteins have the same response ... This notice reports that biuret reacts with alkaline copper sulfate to produce a red solution -- the so-called "Biuret test" ...
Biuret amidohydrolase - Wikipedia
In enzymology, a biuret amidohydrolase (EC 3.5.1.84) is an enzyme that catalyzes the chemical reaction biuret + H2O ⇌ {\ ... The systematic name of this enzyme class is biuret amidohydrolase. This enzyme participates in atrazine degradation. Cook AM, ... displaystyle \rightleftharpoons } urea(CH4N2O) + CO2 + NH3 Thus, the two substrates of this enzyme are biuret and H2O, whereas ...
Biuret | Definition of Biuret at Dictionary.com
Biuret definition, a white crystalline substance, C2H5O2N3⋅H2O, soluble in water and alcohol, used for the identification of ... biuret. bituminous, bituminous coal, bitzer, biunique, biuniqueness, biuret, biuret reaction, bivalence, bivalent, bivalent ... biuret. *. The absence of a biuret reaction would point in that direction and the question must await further study. ... On now testing a portion of the clear filtrate for peptone by the biuret test, not a trace of a reaction could be obtained. ...
Biuret dictionary definition | biuret defined
... plural biurets) 1. (organic chemistry) The compound formed by condensation of two molecules of urea with the loss of a molecule ... biuret. Noun (plural biurets). *(organic chemistry) The compound formed by condensation of two molecules of urea with the loss ... Biuret (allophanamide), NH 2 CO NH CO NH 2, is formed by heating urea; by the action of ammonia on allophanic ester; and by ... How would you define biuret? Add your definition here.. Please enable JavaScript to view the comments powered by Disqus.. ...
Biuret - Substance Information - ECHA
Harmonised classification and labelling is a legally binding classification and labelling for a substance, agreed at European Community level. Harmonisation is based on the substances physical, toxicological and eco-toxicological hazard assessment. The Hazard classification and labelling section uses the signal word, pictogram(s) and hazard statements of the substance under the harmonised classification and labelling (CLH) as its primary source of information.. If the substance is covered by more than one CLH entry (e.g. disodium tetraborate EC no. 215-540-4, is covered by three harmonisations: 005-011-00-4; 005-011-01-1 and 005-011-02-9), CLH information cannot be displayed in the InfoCard as the difference between the CLH classifications requires manual interpretation or verification. If a substance is classified under multiple CLH entries, a link to the C&L Inventory is provided to allow users to view CLH information associated with the substance, instead of having the information ...
TDI Biuret - Registration Dossier - ECHA
Hydrolysis: Hydrolysis of the test item is rapidly completed and neither the determination of hydrolysis rates nor the detection of hydrolysis products was possible. No test conditions could be found enabling the performance of a hydrolysis study for the substance according to OECD TG 111 (2004) (Currenta, 2010a). Biodegradation: Ready biodegradability: Within 28 days, a degradation of 0 % was determined for the substance. The substance is considered to be not readily biodegradable. (Currenta, 2009a) Inherent biodegradability: Within 27 days, a degradation of 11 % was determined for the substance. The substance is considered to be not inherently biodegradable. (Currenta, 2010b) Bioaccumulation The substance is characterised by estimated bioconcentration factors between 1.1 and 25920 covering BCF values of the parent compounds (isocyanate groups) and possible hydrolysis products (amine groups), respectively (Currenta, 2010d). Due to the chemical nature of the parent compounds (isocyanate groups) ...
Chelation Ion Exchange Properties of 2, 4-Dihydroxyacetophenone-Biuret-Formaldehyde Terpolymer Resin
... and biuret (B) with formaldehyde (F) in 1:1:2 molar ratios in presence of 2 M hydrochloric acid as catalyst. UV-Visible, IR and ... Chelation Ion Exchange Properties of 2, 4-Dihydroxyacetophenone-Biuret-Formaldehyde Terpolymer Resin,. Journal of Chemistry,. ... The terpolymer resin 2, 4-HABF has been synthesized by the condensation of 2, 4-dihydroxyacetophenone (2, 4-HA) and biuret (B) ... Chelation Ion Exchange Properties of 2, 4-Dihydroxyacetophenone-Biuret-Formaldehyde Terpolymer Resin. ...
Biuret Reaction | Harvard Catalyst Profiles | Harvard Catalyst
"Biuret Reaction" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Biuret Reaction" by people in Harvard Catalyst Profiles by ... Below are the most recent publications written about "Biuret Reaction" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Biuret Reaction". ...
Biuret Test for Proteins - Stock Image - C039/1301 - Science Photo Library
The Biuret reagent contains copper(II) sulfate (CuSO4) and sodium hydroxide (NaOH), with potassium sodium tartrate (KNaC4H4O6) ... Biuret test for proteins. The Biuret reagent contains copper(II) sulfate (CuSO4) and sodium hydroxide (NaOH), with potassium ... Here blue-coloured Biuret reagent was added to a solution of albumin (egg white) in a test tube. The solution turned violet ( ...
CISDOC - Exposure, lung function, and symptoms in car painters exposed to hexamethylene diisocyanate and biuret modified...
The mean exposure to biuret-modified hexamethylene diisocyanate (HDI-BT) was 115 g/m3 in the air (range 10-385 g/m3), which ... Exposure, lung function, and symptoms in car painters exposed to hexamethylene diisocyanate and biuret modified hexamethylene ... hexamethylene diisocyanate; hexamethylene diisocyanate biuret; symptoms; exposure; vehicle repair and servicing; respiratory ...
Biuret reaction | definition of biuret reaction by Medical dictionary
... biuret reaction explanation free. What is biuret reaction? Meaning of biuret reaction medical term. What does biuret reaction ... Looking for online definition of biuret reaction in the Medical Dictionary? ... biuret test either of the tests done with biuret.. bi·u·ret re·ac·tion. the formation of biuret that gives a violet color as a ... biuret reaction. Also found in: Dictionary, Encyclopedia. biuret. [bi´u-ret] a urea derivative; its presence is detected after ...
β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret) | Sigma-Aldrich
Protein Test - Biuret Reagent Solution, 4oz. for sale. Buy from The Science Company.
The characteristic color of a positive biuret test.. By Ozone aurora/Philip Evans - Own work, CC BY-SA 3.0, Link Protein Test ... Biuret Reagent is an aqueous solution of potassium sodium tartrate treated with cupric sulfate and sodium hydroxide. In the ... Add 3 drops of Biuret reagent solution to each test tube. Shake gently to mix. ... Biuret solution. *To a test tube, add 40 drops of liquid to be tested. ...
Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage: Daily and Alternate Day Supplementation | EOARC -...
Comparison of Urea and Biuret as Nitrogen Supplements to Low-Quality Forage: Daily and Alternate Day Supplementation. Site of ... In addition, duodenal bacterial N flow was increased with CP supplementation (P = 0.04) and for biuret compared with urea (P , ... These results suggest that urea or biuret can be used effectively as a supplemental N source by steers consuming low-quality ... Daily and alternate-day supplementation of urea or biuret to ruminnts consuming low-quality forage: II Effects on site of ...
Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and...
1. Biuret test Biuret test determines the presence of protein by showing the color purple. The results were following: § Table ... The chemical tests that are used for the experiments are: Benedict test, Biuret test and Iodine test, which test for glucose, ... Determine the presence of reducing sugar, protein and starch in the food products by using Benedict test, Biuret test and ... www.scumdoctor.com/nutrition/protein/Biuret-Test-For-Proteins.html - 21/10/10 [5] ?Iodine Test? by Rimlee Bhuyan - 7/23/2010 ? ...
Biuret | definition of biuret by Medical dictionary
... biuret explanation free. What is biuret? Meaning of biuret medical term. What does biuret mean? ... Looking for online definition of biuret in the Medical Dictionary? ... Related to biuret: Biuret test, Biuret reagent. biuret. [bi´u-ret] a urea derivative; its presence is detected after addition ... biuret test either of the tests done with biuret.. bi·u·ret. (bī-yū-ret), A derivative of urea obtained by heating, ...
Cyanuric acid biodegradation via biuret: Physiology, taxonomy, and geospatial distribution<...
The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ... The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). The prevalence of the ...
Biuret
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes. Extinguishing media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire. In case of fire use water spray, dry chemical, carbon dioxide, or appropriate foam ...
Biuret test
... The biuret test, also known as Piotrowskis test, is a chemical test used for detecting the presence of peptide ... Biuret reagent. The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate, together with potassium ... High sensitivity variants of the biuret test. Two major modifications of the biuret test are commonly applied in modern ... Despite its name, the reagent does not in fact contain biuret ((H2N-CO-)2NH). The test is named so because it also gives a ...
ENSAYO DE BIURET PDF
... - The categories of this image should be checked. Check them now! Remove redundant categories and try to ... carbón bituminoso biuret biuret (carbamilurea) biuretamidine sulfate sulfato de test ensayo en blanco blasticidin S ... Biuret is also used as a non-protein nitrogen source in ruminant feed, [8] where it is converted into protein by gut ... File:Reactivo de Biuret.svg. This page was last edited on 16 Novemberat Overexpression of DNA methyltransferase 1 and its ...
Biuret Reagent, 30 ml</span>
Find the biuret reagent form, shelf life, hazard information, and more below. ... This 30 ml bottle of biuret reagent is a qualitative indicator for protein. ... This 30 ml bottle of biuret reagent is a qualitative indicator for protein. Find the biuret reagent form, shelf life, hazard ... This 30 ml bottle of biuret reagent is a qualitative indicator for protein. Find the biuret reagent form, shelf life, hazard ...
What is the reaction of K + H2O? | Reference.com
What is "ferrous sulfate 325 mg?" | Reference.com
Sensors | Free Full-Text | Multi-instrumental Analysis of Tissues of Sunflower Plants Treated with Silver(I) Ions - Plants as...
The measurement was done as follows: 180 μl of the biuret solution was mixed with 45 μl of real or standard sample; after ... For determination of the total protein content the biuret solution (15 mM potassium sodium tartrate, 100 mM NaI, 15 mM KI and 5 ... At the plants treated with silver(I) ions the changes were observable also in total content of proteins determined using biuret ... 2.4.2 Protein determination - Biuret test. Weighed plant tissues (approximately 0.2 g) were transferred to a test-tube. Then, ...
Buy Low Biuret Prilled Urea 46% - Biochar Organic Fertilizer Manufacture
HS-797/CH142: A Guide to Citrus Nutritional Deficiency and Toxicity Identification
Indicators and Dyes for Chemical Processing - Alfa Aesar
Biuret Toxicity Symptoms in Citrus Leaves Mimics Cell Senescence Rather Than Nutritional Deficiency Chlorosis in: Journal of...
Biuret Toxicity Symptoms in Citrus Leaves Mimics Cell Senescence Rather Than Nutritional Deficiency Chlorosis. in Journal of ... Concurrent loss of cytoplasmic content and the enlargement of the vacuolar space were also observed in the biuret affected leaf ... Permanent chlorosis of leaves on plants fertilized with urea containing high levels of the contaminant biuret has been observed ... It was concluded that 1) the cytological characteristics of the biuret-affected samples were more similar to age-related ...
IUCr) Acta Crystallographica Volume 20, Part 2, February 1966
Reagent17
- It is based on the biuret reagent, a blue solution that turns violet upon contact with proteins, or any substance with peptide bonds. (wikipedia.org)
- The Biuret reagent contains copper(II) sulfate (CuSO4) and sodium hydroxide (NaOH), with potassium sodium tartrate (KNaC4H4O6) added to stabilize copper ions. (sciencephoto.com)
- Here blue-coloured Biuret reagent was added to a solution of albumin (egg white) in a test tube. (sciencephoto.com)
- Biuret Reagent Solution, 4oz. (sciencecompany.com)
- Biuret Reagent Solution, 125mL (4 oz. (sciencecompany.com)
- Biuret Reagent is an aqueous solution of potassium sodium tartrate treated with cupric sulfate and sodium hydroxide. (sciencecompany.com)
- Add 3 drops of Biuret reagent solution to each test tube. (sciencecompany.com)
- Despite its name, the reagent does not in fact contain biuret ((H 2 N-CO-) 2 NH). (orange.com)
- The Biuret reagent is made of sodium hydroxide (NaOH) and hydrated copper(II) sulfate , together with potassium sodium tartrate , [5] the latter of which is added to chelate and thus stabilize the cupric ions. (orange.com)
- The reagent is commonly used in the biuret protein assay , a colorimetric test used to determine protein concentration by UV/VIS spectroscopy at wavelength 540 nm. (orange.com)
- This 30 ml bottle of biuret reagent is a qualitative indicator for protein. (homesciencetools.com)
- What is a biuret reagent? (reference.com)
- A biuret reagent is a mix of either sodium hydroxide or potassium hydroxide and copper sulfate. (reference.com)
- The reagent is used in the biuret test, which determines wh. (reference.com)
- Biuret is a method in which a reagent turns from green to violet when it comes into contact with certain amino acids (a certain sign of protein presence). (foodsafetymagazine.com)
- Biuret reagent to test for proteins. (mcgill.ca)
- The Biuret reagent is made of sodium hydroxide and copper sulphate. (markedbyteachers.com)
Reaction22
- The absence of a biuret reaction would point in that direction and the question must await further study. (dictionary.com)
- Amongst these is the biuret reaction: a pink colour obtained by adding a trace of copper sulphate and an excess of caustic soda. (dictionary.com)
- On now testing a portion of the clear filtrate for peptone by the biuret test, not a trace of a reaction could be obtained. (dictionary.com)
- Inasmuch as most natural proteins contain several such groups, the biuret reaction is a very general test for proteins. (dictionary.com)
- In enzymology, a biuret amidohydrolase (EC 3.5.1.84) is an enzyme that catalyzes the chemical reaction biuret + H2O ⇌ {\displaystyle \rightleftharpoons } urea(CH4N2O) + CO2 + NH3 Thus, the two substrates of this enzyme are biuret and H2O, whereas its 3 products are urea, CO2, and NH3. (wikipedia.org)
- and by the so-called " biuret " reaction. (yourdictionary.com)
- Biuret Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- This graph shows the total number of publications written about "Biuret Reaction" by people in Harvard Catalyst Profiles by year, and whether "Biuret Reaction" was a major or minor topic of these publication. (harvard.edu)
- Below are the most recent publications written about "Biuret Reaction" by people in Profiles. (harvard.edu)
- Thus single AMINO ACIDS and DIPEPTIDES with only one peptide bond will not give a positive Biuret reaction. (thefreedictionary.com)
- Ethanolamine serves as a model for the chelate-forming portions of threonine and serine, and its reactivity in the biuret reaction was of similar magnitude. (thefreedictionary.com)
- This suggests that absorbance for the biuret reaction product of albumin was the sum of peptide backbone and sidechain interactions with copper. (thefreedictionary.com)
- The biuret reaction of amino acids and small peptides was completed by the time of the first postbaseline reading: within ~2-4 s depending on the speed of mixing. (thefreedictionary.com)
- The biuret reaction commonly has been considered to selectively measure peptides 3 amino acid residues in length or longer (3), although there have been reports extending back many years indicating that amino acids, dipeptides, and other compounds are reactive (30-33). (thefreedictionary.com)
- The reaction generated biuret, a pathway intermediate further transformed by biuret hydrolase (BiuH). (umn.edu)
- The biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptide. (orange.com)
- The test is named so because it also gives a positive reaction to the peptide-like bonds in the biuret molecule. (orange.com)
- In these tests, the Cu + formed during the biuret reaction reacts further with other reagents, leading to a deeper color. (orange.com)
- Crystallographic studies of the biuret reaction. (iucr.org)
- The biuret reaction has been in use since the end of the 19th century and is the method of choice in clinical laboratories because of its simplicity, rapidity and reliability. (rushtermpapers.com)
- The peptide bonds of protein react with the copper II ions in alkaline solution to form a blue-violet complex (the so-called biuret reaction), each copper ion complexing with 5 or 6 peptide bonds. (rushtermpapers.com)
- and caustic potash and copper sulphate, which, on warming, gives a red to violet coloration (biuret reaction). (wikisource.org)
Proteins8
- The biuret test is a chemical test for proteins and polypeptides. (wikipedia.org)
- they are so named because both biuret and proteins have the same response to the test. (wikipedia.org)
- Very few laboratories use the biuret method, which reacts with the peptide bonds in proteins, measuring all proteins more equally. (thefreedictionary.com)
- The water-soluble BCA/copper complex absorbs much more strongly than the peptide/copper complex, increasing the sensitivity of the biuret test by a factor of around 100: the BCA assay allows to detect proteins in the range of 0.0005 to 2 mg/mL). (orange.com)
- Mitotic recombination in patients with ichthyosis causes reversion of dominant mutations in KRT It is based on the biuret reagenta blue solution that turns violet upon contact with proteins, or any substance with peptide bonds. (iamadek.me)
- A biuret solution is a liquid mixture of potassium hydroxide and copper sulfate used to detect proteins and peptides in human urine. (reference.com)
- A micro-biuret method for estimating proteins. (stackexchange.com)
- If you want to find out is there are proteins in a substance then you would use the biuret test. (getrevising.co.uk)
Assay4
- 1, as measured by the biuret assay, the pyrogallol red assay, and nephelometry. (thefreedictionary.com)
- The calibration curve for the Biuret assay, made with human albumin concentrations of 0-10 g/L, gave an absorbance range (550 nm) of 0. (thefreedictionary.com)
- Two major modifications of the biuret test are commonly applied in modern colorimetric analysis of peptides: the bicinchoninic acid (BCA) assay and the Lowry assay. (orange.com)
- Classical methods such as the biuret test, Bradford test, spectrophotometric assay at 280 nm, Smith test, and Lowry test are some of the most commonly used techniques in protein quantitation. (slideshare.net)
Grade Biuret3
- Feed Grade Biuret as a Protein Replacement for Ruminants. (wikipedia.org)
- Feed-grade biuret. (ecfr.gov)
- Cyanuric acid is an FDA-accepted component of feed-grade biuret, a ruminant feed additive. (medicalnewstoday.com)
Compared with urea1
- Bacterial efficiency (g bacterial N/kg OM truly digested in the rumen) was greater ( P = 0.05) for biuret compared with urea. (oregonstate.edu)
Test17
- This notice reports that biuret reacts with alkaline copper sulfate to produce a red solution -- the so-called "Biuret test" Wiedemann, G. (1848). (wikipedia.org)
- The characteristic color of a positive biuret test. (sciencecompany.com)
- The chemical tests that are used for the experiments are: Benedict test, Biuret test and Iodine test, which test for glucose, protein and starch. (markedbyteachers.com)
- The biuret test , also known as Piotrowski's test , is a chemical test used for detecting the presence of peptide bonds . (orange.com)
- In Poland , the biuret test is more commonly known as the Piotrowski test, in honor of the Polish toxological chemist, Jerzy Kazimierz Piotrowski (b. 1926) of Lodz . (orange.com)
- carbón bituminoso biuret biuret (carbamilurea) biuretamidine sulfate sulfato de test ensayo en blanco blasticidin S blasticidina S bleach blanqueo bleaching. (iamadek.me)
- Why do protein solutions have to be alkalised in biuret test? (stackexchange.com)
- I've never done the biuret test myself, but I can't imagine it would make a difference. (stackexchange.com)
- To avoid these problems, the Biuret test was developed. (markedbyteachers.com)
- Biuret test is of particular interest in this study. (slideshare.net)
- However, similar with other protein assays, the linear range for the Biuret test found in different literature varies. (slideshare.net)
- The most common lover limit of the calibration curve for the Biuret test is 1mg/sample. (slideshare.net)
- Biuret Test - Protein - When the Biuret solution is added to the protein solution, it produces a purple solution, while all the other macromolecules with the Biuret solution produce a blue tinted solution. (majortests.com)
- The Biuret Test displayed a light/dark blue solution. (majortests.com)
- The Biuret test with the unknown produced a separated light/dark blue solution like Unknown #20. (majortests.com)
- This lab is broken down into four different sections, the Benedict's test for reducing sugars, the iodine test for the presence of starch, the Sudan III test for fatty acids, and the Biuret test for amino groups present in proteins. (artscolumbia.org)
- Biuret test is done for? (proprofs.com)
Cyanuric4
- Cyanuric acid Allophanic acid, the carboxylic acid derivative of biuret Nomenclature of Organic Chemistry : IUPAC Recommendations and Preferred Names 2013 (Blue Book). (wikipedia.org)
- When heated above its melting-point, it yields ammonia, cyanuric acid, biuret and ammelide. (yourdictionary.com)
- Here, we show that most bacteria utilize a different pathway, metabolizing cyanuric acid via biuret. (umn.edu)
- Mining a database of over 40,000 bacterial isolates with precise geospatial metadata showed that bacteria with concurrent cyanuric acid and biuret hydrolase genes were distributed throughout the United States. (umn.edu)
Peptide1
- IMSEAR at SEARO: Determination of dipeptides in peptide mixtures using a simple biuret method. (who.int)
Isocyanurate3
- Examples of widely used polyisocyanates include HDI biuret and HDI isocyanurate. (cdc.gov)
- Methods: Exposures to aliphatic isocyanates based on 1,6-hexamethylene diisocyanate (1,6-HDI) and its higher oligomers (biuret, isocyanurate and uretdione) were measured among 30 workers performing painting of bridges and other metal structures in several construction sites in the Northeastern USA. (cdc.gov)
- Results: Breathing zone concentrations were the highest for biuret (median, 18.4 microg/m3), followed by 1,6-HDI monomer (median, 3.5 microg/m3), isocyanurate (median, 3.4 microg/m3) and uretdione (median, 1.7 microg/m3). (cdc.gov)
Sensitivity1
- Determination of total urinary protein, combining Lowry sensitivity and biuret specificity. (thefreedictionary.com)
Reagents1
- Commercially available biuret reagents are sold as premixed solutions. (stackexchange.com)
Assays1
- Most of these methods rely either on colorimetric assays, e.g. biuret method or the use of UV absorbance method. (rushtermpapers.com)
Acid1
- The terpolymer resin 2, 4-HABF has been synthesized by the condensation of 2, 4-dihydroxyacetophenone (2, 4-HA) and biuret (B) with formaldehyde (F) in 1:1:2 molar ratios in presence of 2 M hydrochloric acid as catalyst. (hindawi.com)
Nitrogen1
- Biuret is also used as a non-protein nitrogen source in ruminant feed, where it is converted into protein by gut microorganisms. (wikipedia.org)
Reactions1
- They give the biuret and xanthoproteic reactions, and form salts with both acids and bases. (yourdictionary.com)
Method4
- In addition, these authors found a good correlation between the Vitros method and the biuret method, taking the latter as reference. (thefreedictionary.com)
- The protein concentration of the myofibril solution was determined by the biuret method (Gornall, Bardawill, & Maxima, 1949). (thefreedictionary.com)
- Determine the concentration of an unknown protein solution using two different spectroscopic methods (the biuret method and UV method) for the measurement of protein concentration. (rushtermpapers.com)
- In this practical, you will determine the concentration of an unknown protein solution using two different spectroscopic methods (the biuret method and UV method) for the measurement of protein concentration. (rushtermpapers.com)
Urinary1
- Methods for the determination of total urinary protein that use precipitation of protein and indirect biuret are accurate, but they also are time-consuming and, therefore, not widely used. (thefreedictionary.com)
Starch1
- The progressive development of chlorosis in biuret-affected leaves was characterized by: the loss of starch, thylakoidal and granal membranes in chloroplasts along with the enlargement and increase in number of plastoglobuli or lipid bodies. (ashs.org)
Solution2
- What is a biuret solution? (reference.com)
- The purple solution comes from the reduction of Cu in the Biuret solution. (majortests.com)
Characteristics1
- It was concluded that 1) the cytological characteristics of the biuret-affected samples were more similar to age-related senescent samples than to chlorosis from Zn deficiency and 2) that complete loss of the lipid bodies from the chromoplasts to the cytoplasm and vacuole in the biuret-affected samples and in age-related senescence in citrus leaves was responsible for the permanent nature of the chlorosis. (ashs.org)
Formula1
- Biuret is a chemical compound with the chemical formula [H2NC(O)]2NH. (wikipedia.org)
Product1
- Zersetzungsprodukt des Harnstoffs" [Biuret: decomposition product of urea]. (wikipedia.org)
Solutions1
- Branches from `Ruby Red' grapefruit ( Citrus paradisi Macfadyn) and `Hamlin' orange [ C. sinensis (L.) Osbeck] were sprayed with urea solutions containing 1.05% biuret. (ashs.org)
Groups1
- In general, organic biurets (those with alkyl or aryl groups in place of one or more H atoms) are prepared by trimerization of isocyanates. (wikipedia.org)
Description1
- Summary [ edit ] Description Reactivo de Biuret. (iamadek.me)