The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Promoter Regions, Genetic
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Directories as Topic
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.
Extensive collections, reputedly complete, of facts and data garnered from material of a specialized subject area and made available for analysis and application. The collection can be automated by various contemporary methods for retrieval. The concept should be differentiated from DATABASES, BIBLIOGRAPHIC which is restricted to collections of bibliographic references.
Cell Cycle Proteins
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Encyclopedias as Topic
Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Sensitivity and Specificity
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Journal Impact Factor
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans. (1/182)In the filamentous fungus Aspergillus nidulans, germination of an asexual conidiospore results in the formation of a hyphal cell. A key feature of spore germination is the switch from isotropic spore expansion to polarized apical growth. Here, temperature-sensitive mutations are used to characterize the roles of five genes (sepA, hypA, podB-podD) in the establishment and maintenance of hyphal polarity. Evidence that suggests that the hypA, podB, and sepA genes are required for multiple aspects of hyphal morphogenesis is presented. Notably, podB and sepA are needed for organization of the cytoskeleton at sites of polarized growth. In contrast, podC and podD encode proteins that appear to be specifically required for the establishment of hyphal polarity during spore germination. The role of sepA and the pod genes in controlling the spatial pattern of polarized morphogenesis in germinating spores is also described. Results obtained from these experiments indicate that the normal pattern of germ-tube emergence is dependent upon the integrity of the actin cytoskeleton. (+info)
The effect of mannitol versus dimethyl thiourea at attenuating ischemia/reperfusion-induced injury to skeletal muscle. (2/182)OBJECTIVE: Mannitol is used as a treatment for skeletal muscle ischemia/reperfusion (I/R) injury in humans, despite the fact that its effectiveness in vivo is still disputed. The purpose of this study was to determine the efficacy of mannitol in attenuating I/R injury at the microcirculatory level. METHODS: The study was designed as an experimental study with male Wistar rats. The main outcome measures were intravital microscopy, which was used to measure capillary perfusion, capillary and venular red blood cell velocity (VRBC), and leukocyte-endothelial interactions in the extensor digitorum longus muscle of the rat hind limb before and after ischemia. In addition, tissue injury was assessed during reperfusion with the fluorescent vital dyes bisbenzimide and ethidium bromide. Dimethyl thiourea (DMTU), a highly effective therapeutic agent of experimental I/R injury, was used as a positive control. RESULTS: No-flow ischemia (2 hour) resulted in a 40% drop in capillary perfusion, a decline in capillary and venular VRBC, and increased leukocyte venular adherence and tissue infiltration. Tissue injury increased to a constant level during reperfusion. Mannitol attenuated capillary malperfusion during the first 60 minutes of reperfusion and prevented a decline in capillary VRBC. However, mannitol did not reduce tissue injury or leukocyte adherence and infiltration during reperfusion. By comparison, DMTU not only prevented the perfusion deficits and the increases in leukocyte venular adherence and tissue infiltration but significantly reduced the magnitude of tissue injury. CONCLUSION: Our findings suggest that mannitol may be of limited value for the prevention of early reperfusion-induced injury after no-flow ischemia in skeletal muscle. By comparison, DMTU was highly efficacious by not only reducing microvascular perfusion deficits but by also reducing leukocyte-endothelial cell interactions and the incidence of cellular injury. (+info)
DNA minor groove recognition by bis-benzimidazole analogues of Hoechst 33258: insights into structure-DNA affinity relationships assessed by fluorescence titration measurements. (3/182)Fluorescence titration measurements have been used to examine the binding interaction of a number of analogues of the bis -benzimidazole DNA minor groove binding agent Hoechst 33258 with the decamer duplex d(GCAAATTTGC)2. The method of continuous variation in ligand concentration (Job plot analysis) reveals a 1:1 binding stoichiometry for all four analogues; binding constants are independent of drug concentration (in the range [ligand] = 0.1-5 microM). The four analogues studied were chosen in order to gain some insight into the relative importance of a number of key structural features for minor groove recognition, namely (i) steric bulk of the N -methylpiperazine ring, (ii) ligand hydrophobicity, (iii) isohelicity with the DNA minor groove and (iv) net ligand charge. This was achieved, first, by replacing the bulky, non-planar N -methylpiperazine ring with a less bulky planar charged imidazole ring permitting binding to a narrower groove, secondly, by linking the N -methylpiperazine ring to the phenyl end of the molecule to give the molecule a more linear, less isohelical conformation and, finally, by introducing a charged imidazole ring in place of the phenolic OH making it dicationic, enabling the contribution of the additional electrostatic interaction and extended conformation to be assessed. Delta G values were measured at 20 degrees C in the range -47.6 to -37.5 kJ mol-1 and at a number of pH values between 5.0 and 7.2. We find a very poor correlation between Delta G values determined by fluorescence titration and effects of ligand binding on DNA melting temperatures, concluding that isothermal titration methods provide the most reliable method of determining binding affinities. Our results indicate that the bulky N -methylpiperazine ring imparts a large favourable binding interaction, despite its apparent requirement for a wider minor groove, which others have suggested arises in a large part from the hydrophobic effect. The binding constant appears to be insensitive to the isohelical arrangement of the constituent rings which in these analogues gives the same register of hydrogen bonding interactions with the floor of the groove. (+info)
Progesterone promotes the acrosome reaction in capacitated human spermatozoa as judged by flow cytometry and CD46 staining. (4/182)The acrosome reaction is a necessary prerequisite for spermatozoa to acquire fertilizing ability. Several different moieties appear to promote the acrosome reaction through different pathways, including solubilized zona pellucidae, recombinant zona protein ZP3, follicular fluid, calcium ionophores, and mannosylated bovine serum albumin (BSA). Although many investigators have presented evidence that progesterone also promotes the acrosome reaction through the mediation of a non-genomic cell membrane receptor, this concept has been challenged. Other workers have suggested that progesterone does not promote an acrosome reaction in human spermatozoa, as judged by the detection of CD46, a complement regulatory protein present on the inner acrosome membrane, through flow cytometric analysis of large numbers of spermatozoa. Prior investigations were criticized by the limited numbers of spermatozoa enumerated visually, the use of non-specific staining techniques, and the failure to eliminate dead spermatozoa during the scoring of the acrosome reaction. We have repeated these experiments, using both a supravital dye to eliminate dead spermatozoa from flow cytometric analysis, and anti-CD46 monoclonal antibody to score acrosome-reacted spermatozoa. Care was taken to validate the adequacy of capacitation conditions, which were proven by the ability of spermatozoa to acrosome react in response to mannosylated BSA and to penetrate zona-free hamster eggs. Confocal microscopy was used to confirm that CD46 immunostaining was limited to the acrosomal region of the spermatozoon head. Our results indicate that progesterone does promote an acrosome reaction within capacitated spermatozoa. (+info)
Autosomal trisomy 20 (61,XX,+20) in a malformed bovine fetus. (5/182)A 240-day-gestation female bovine fetus with severe anasarca, palatoschisis, cheiloschisis, mild cranioschisis, and a flattened facies was collected at a slaughterhouse, and a fibroblast line was established from the fetal skin. Chromosome preparations were Q-banded, and chromosome counts were taken that indicated the presence of 61 chromosomes in cells of the fetus (the normal diploid number for domestic cattle is 60). Q-band karyotypes were constructed, and Q-band analysis revealed the presence of three copies of chromosome 20. Trisomy 20 (61,XX,+20) was confirmed through the use of two-color fluorescence in situ hybridization of bovine bacterial artificial chromosome clones that were specific to chromosome 20 and the X chromosome. (+info)
Massive parallel analysis of DNA-Hoechst 33258 binding specificity with a generic oligodeoxyribonucleotide microchip. (6/182)A generic oligodeoxyribonucleotide microchip was used to determine the sequence specificity of Hoechst 33258 binding to double-stranded DNA. The generic microchip contained 4096 oxctadeoxynucleo-tides in which all possible 4(6)= 4096 hexadeoxy-nucleotide sequences are flanked on both the 3'- and 5'-ends with equimolar mixtures of four bases. The microchip was manufactured by chemical immobilization of presynthesized 8mers within polyacrylamide gel pads. A selected set of immobilized 8mers was converted to double-stranded form by hybridization with a mixture of fluorescently labeled complementary 8mers. Massive parallel measurements of melting curves were carried out for the majority of 2080 6mer duplexes, in both the absence and presence of the Hoechst dye. The sequence-specific affinity for Hoechst 33258 was calculated as the increase in melting temperature caused by ligand binding. The dye exhibited specificity for A:T but not G:C base pairs. The affinity is low for two A:T base pairs, increases significantly for three, and reaches a plateau for four A:T base pairs. The relative ligand affinity for all trinucleotide and tetranucleotide sequences (A/T)(3)and (A/T)(4)was estimated. The free energy of dye binding to several duplexes was calculated from the equilibrium melting curves of the duplexes formed on the oligonucleotide microchips. This method can be used as a general approach for massive screening of the sequence specificity of DNA-binding compounds. (+info)
Synergistic cytotoxicity and apoptosis by Apo-2 ligand and adriamycin against bladder cancer cells. (7/182)Resistance to conventional anticancer chemotherapeutic agents remains one of the major problems in the treatment of bladder cancer. Hence, new therapeutic modalities are necessary to treat the drug-resistant cancers. Apo-2 ligand (Apo-2L) is member of the tumor necrosis factor ligand family, and it induces apoptosis in cancer cells. Several cytotoxic anticancer drugs, including Adriamycin (ADR), also mediate apoptosis and may share the common intracellular pathways leading to cell death. We reasoned that combination treatment of the drug-resistant cancer cells with Apo-2L and drugs might overcome their resistance. Here, we examined whether bladder cancer cells are sensitive to Apo-2L-mediated cytotoxicity and whether Apo-2L can synergize with ADR in cytotoxicity and apoptosis against bladder cancer cells. Recombinant human soluble Apo-2L (sApo-2L), which carries the extracellular domain of Apo-2L, was used as a ligand. Cytotoxicity was determined by a 1-day microculture tetrazolium dye assay. Synergy was assessed by isobolographic analysis. Human T24 bladder cancer line was relatively resistant to sApo-2L. Treatment of T24 line with combination of sApo-2L and ADR resulted in a synergistic cytotoxic effect. Synergy was also achieved in the ADR-resistant T24 line (T24/ADR), two other bladder cancer lines, and three freshly derived human bladder cancer cell samples. In addition, T24 cells were sensitive to treatment with sApo-2L combined with epirubicin or pirarubicin. The synergy achieved in cytotoxicity with sApo-2L and ADR was also achieved in apoptosis. Intracellular accumulation of ADR was not affected by sApo-2L. Incubation of T24 cells with sApo-2L down-regulated the expression of glutathione S-transferase-pi mRNA. This study demonstrates that combination treatment of bladder cancer cells with sApo-2L and ADR overcomes their resistance. The sensitization obtained with established ADR-resistant bladder cancer cells and freshly isolated bladder cancer cells required low subtoxic concentrations of ADR, thus supporting the in vivo potential application of combination of sApo-2L and ADR in the treatment of ADR-resistant bladder cancer. (+info)
DNA cleavage by hydroxy-salicylidene-ethylendiamine-iron complexes. (8/182)Bis(hydroxy)salen.Fe complexes were designed as self-activated chemical nucleases. The presence of a hy-droxyl group on the two salicylidene moieties serve to form a hydroquinone system cooperating with the iron redox system to facilitate spontaneous formation of free radicals. We compared the DNA binding and cleaving properties of the ortho -, meta- and para -(bishydroxy) salen.Fe complexes with that of the corresponding chelate lacking the hydroxyl groups. DNA melting temperature studies indicated that the para complex exhibits the highest affinity for DNA. In addition, this para compound was considerably more potent at cleaving supercoiled plasmid DNA than the regio-isomeric ortho - and meta -hydroxy-salen.Fe complexes, even in the absence of a reducing agent, such as dithiothreitol used to activate the metal complex. The DNA cleaving activity of the para isomer is both time and concentration dependent and the complexed iron atom is absolutely essential for the sequence uniform cleavage of DNA. From a mechanistic point of view, electron spin resonance measurements suggest that DNA contributes positively to the activation of the semi-quinone system and the production of ligand radical species responsible for subsequent strand scission in the absence of a reducing agent. The para -hydroxy-salen.Fe complex has been used for detecting sequence-specific drug-DNA interactions. Specific binding of Hoechst 33258 to AT sequences and chromomycin to GC sequences were shown. The para -bis(hydroxy)salen.Fe derivative complements the tool box of footprinting reagents which can be utilised to produce efficient cleavage of DNA. (+info)
Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer...
Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer Activity
ChemIDplus - 23491-44-3 - INAAIJLSXJJHOZ-UHFFFAOYSA-N - Bisbenzimidazole - Similar structures search, synonyms, formulas,...
23491-44-3 - INAAIJLSXJJHOZ-UHFFFAOYSA-N - Bisbenzimidazole - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Imidazole-imidazole pair as a minor groove recognition motif for T:G mismatched base pairs
The T:G mismatched base pair is associated with many genetic mutations. Understanding its biological consequences may be aided by studying the structural perturbation of DNA caused by a T:G base pair and by specific probing of the mismatch using small molecular ligands. We have shown previously that AR-1-144, a tri-imidazole (Im-Im-Im) minor groove binder, recognizes the sequence CCGG. NMR structural analysis of the symmetric 2:1 complex of AR-1-144 and GAACCGGTTC revealed that each AR-1-144 binds to four base pairs with the guanine N2 amino group forming a bifurcated hydrogen bond to a side-by-side Im/Im pair. We predicted that the free G-N2 amino group in a T:G wobble base pair can form two individual hydrogen bonds to a side-by-side Im/Im pair. Thus an Im/Im pair may be a good recognition motif for a T:G base pair in DNA. Cooperative and tight binding of an AR-1-144 homodimer to GAACTGGTTC permits a detailed structural analysis by 2D NOE NMR refinement and the refined structure confirms our ...
Structure Cluster - 1D65: MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER...
1D65: Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution.
RCSB PDB - 117D: CRYSTAL AND MOLECULAR STRUCTURE OF THE ALTERNATING DODECAMER D(GCGTACGTACGC) IN THE A-DNA FORM:...
117D: Crystal and molecular structure of the alternating dodecamer d(GCGTACGTACGC) in the A-DNA form: comparison with the isomorphous non-alternating dodecamer d(CCGTACGTACGG).
Cytotoxicity of hoechst staining in overnight experiment - Cell Biology - BioForum
Cytotoxicity of hoechst staining in overnight experiment - posted in Cell Biology: Hi everyone, Has anybody ever done an overnight microscopy acquisition of cells that were stained with hoechst just before starting the experiment? If so, did your cells survived the night? was there a big photo-bleaching of the Hoechst staining? Thank you in advance for your answers Juju
Discovery of N-methylpiperazinyl flavones as a novel class of compounds with therapeutic potential against Alzheimers disease ...
With no currently available disease-modifying drugs, Alzheimers disease is the most common type of dementia affecting over 47 million people worldwide. In light of the most recent discoveries placing the cellular prion protein (PrPC) as a key player in amyloid β oligomer (Aβo)-induced neurodegeneration, we investigated whether the neuroprotective potential of nature-inspired flavonoids against Aβ-promoted toxicity would translate into the ability to disrupt PrPC-Aβo interactions. Hence, we synthesized a small library of flavones and studied their binding affinity towards Aβo by STD-NMR. C-glucosyl flavones exhibited improved binding affinity with morpholine, thiomorpholine or N-methylpiperazine rings attached to the flavone skeleton in ring B para position. Moreover, a N-methylpiperazinyl flavone displayed suitable physicochemical properties and optimal water solubility even without the sugar moiety, and a high interaction with Aβo involving the whole flavone core. Its C-glucosyl ...
Patente US20030087431 - Composite blastocysts (CBs) from aggregates of dissociated cells of non ... - Google Patentes
A preparation and a method of making composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos are disclosed. The CB is characterized morphologically by having two distinct tissue types, the inner cell mass (ICM) and the trophectoderm (TE), and a blastocoelic cavity (BC). The ICM is differentially stainable with bisbenzimide and the TE is differentially stainable with propidium iodide. The ICM is pluripotent in that it contains embryonic stem (ES) cells. The TE cells are pluripotent in that they can give rise to all cell types normally derived from TE cells. The primate TE is characterized by the production of chorionic gonadotrophin. The method of making CBs is an aggregation process (AP) comprising inter alia the following steps: 1) dissociation of discarded pre-embryos; 2) isolation of single nucleated cells from dissociated discarded pre-embryos; 3) microsurgical encapsulation of several cells within a host zona pellucida or artificial aggregation with or without a
British Library EThOS: Synthesis of novel oligomeric bis-benzimidazoles for their biological evaluation
Benzimidazoles are heterocyclic compounds. Symmetrical and unsymmetrical benzimidazoles/oligomers are minor groove DNA sequence selective binding compounds. Distamycin A and netropsin are examples of naturally occurring DNA binders. Hoechst 33258 (Bis-benzimidazole) is a synthetic minor groove A-T sequence selective reagent and has in vivo activity by inhibiting the topoisomerase II enzyme. The targets in this research work were to synthesise extended analogues of Hoechst with structural modifications (amide bond) or amide-linked dimers with a view to identifying new potential ligands. To synthesise a library of novel bis-benzimidazoles (analogues of Hoechst) several methods were used. For C5-C2 direct linkage aldehyde synthesis via ester, Weinreb amide reduction, condensation of acids with diamine by Eatons reagent were applied. Cyclization of amide-linked benzimidazoles (amide bond between carboxylic acid of C5 benzimidazole and diamine), an indirect method of bis-benzimidazole synthesis was ...
52nd asms conference abstracts listing
http://www.asms.org/abstracts/DisplayAbstractList.aspx?Session=MODam (2 of 4) [28-May-05 5:32:36 PM] assess the changes that occurred in the distribution of GPEtn lipids after exposure of liposomes made from phospholipids extracted from RAW 264.7 cells to Cu2+/H2O2. The control liposomes were labeled with the 114 reagent and the Cu2+/H2O2 liposomes were labeled with the 117 reagent. Upon comparison of the precursors of m/z 114 scan to the precursors of m/z 117 scan it was found that the amount of lyso GPEtn increased in the oxidized sample. It has been found that the N-methylpiperazine amide isotope tag is a novel way to observe changes in the distribution of GPEtn and GPSer species, which has been difficult to achieve in the past. Lipidomics of bacterial invasion and dormancy ...
Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid...
Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties
DNA-Complexes with Drugs and Proteins
DNA is fundamental for all living cells; the DNA holds the genetic code, which is more or less the instruction book for how all cells are built and function. Several diseases are also linked to DNA, emerging either from a mutation in our genome, which could result in a malfunctioning protein, or that the transcription of genes is somehow affected by structural changes in the DNA, induced by mutations or DNA binding molecules. Research concerning how both small molecules and larger protein assemblies bind to the DNA are therefore of great interest since these could be used as future drugs in for example gene therapy. In the first part of this Thesis the non-covalent binding to DNA of a small minor groove binder, Hoechst 33258, is examined. The molecule is rather well-studied, but there are still questions concerning its multiple binding modes to DNA sequences rich in adenines (A) and thymines (T) that remain unanswered. An increased understanding of the nature of the multiple binding modes could benefit
DAPI/Hoechst vital staining - Flow Cytometry - BioForum
DAPI/Hoechst vital staining - posted in Flow Cytometry: Hi there; I wanna stain GFP transfected human cells [adhaerent monolayer culture] with DAPI or Hoechst. My problem is that every fixation kills my GFP in the cells. Does anyone know a good protocol for vital staining of the nucleus without any fixation of the cell. Thx Neo
1G3X INTERCALATION OF AN 9ACRIDINE-PEPTIDE DRUG IN A DNA DODECAMER | 1G3X I | P15056 | BRAF | Serine/threonine-protein kinase B...
Fullscreen (supported by IE11, latest versions of Firefox, Chrome, Safari (not including iOS Safari), Edge, Chrome for Android, Samsung Internet) ...
Workshop Conference Hoechst (18th 1987 Schloss Ringberg) | Open Library
Books by Workshop Conference Hoechst (18th 1987 Schloss Ringberg), Surface structures of microorganisms and their interactions with the mammalian host
Top 10 Hotels in Hoechst, Germany | Hotels.com
Compare 2 hotels in Hoechst using 234 real guest reviews. Earn free nights and get our Price Guarantee - booking has never been easier on Hotels.com!
Koerner and Cotman (1981) initially described that the diacidic amino acid analog l-2-amino-4-phosphonobutyric acid (l-AP4) selectively suppressed glutamate excitations by a presynaptic mechanism in the lateral perforant pathway of the hippocampus. This inhibitory activity of l-AP4 on glutamate excitations was also observed in other preparations, including the mossy fiber synapse, lateral olfactory tract, and spinal cord (seeThomsen, 1997). Until the 1990s, presynaptic inhibition induced by l-AP4 was ascribed to a relatively nebulous l-AP4 receptor. However, with the cloning of the group III mGlu receptors, which Nakanishi (1992) defined by their sensitivity to l-AP4, it was recognized that certain group III mGlu subtypes might be responsible forl-AP4-induced suppression of glutamate release. Current data suggest a role for mGlu7, mGlu8, and possibly mGlu4 as candidates for these presynaptic effects of l-AP4 in the brain (seeThomsen, 1997).. In general, when compared with mGlu7 or mGlu2/3 ...
Binding Properties of Large Antiviral Polyamides (PA1 and PA25) and Na by Elena Vasilieva
Polyamides are a class of DNA minor groove binders that were developed from the natural product Distamycin A. PA25 (20-ring PA) showed better activity (3-fold) in the elimination of HPV16 episomes than PA1 (14-ring PA). Binding studies of PA1 and PA25 were performed in the long control region of HPV16 (7348-122 bp). PA1 showed similar binding affinity to perfect match and single mismatch binding sites. PA1 bound to double, triple, and quadruple mismatch sites with lower affinity. PA25 bound with similar binding affinity to perfect through triple mismatch binding sites. PA25 bound quadruple mismatch sites and nonspecifically. Therefore, it was concluded that PA25 is better at accommodating mismatches in its binding sites. The serine hydrolase family has a catalytic triad that consists of Serine, Histidine, and Glutamic/Aspartic Acid. Acetylcholinesterase (AChE) belongs to the serine hydrolase family. AChE hydrolyzes acetylcholine to stop signal transmission between neurons and thus is a drug target for
Bis-benzimidazole derivatives as hepatitis c virus inhibitors
Alternatively, as shown in figure 5, the protective group of compound IX can be removed under conditions that are compatible with the Boc protecting group, e.g. by hydrogenation, when PG is a benzyl or benzyloxycarbonyl, or basic conditions, such as diethylamine, when PG represents fluorenylmethoxycarbonyl, to obtain the compound XVI. Other methods of selective removal of the protective groups can be found in the directory Greene. In this case, the connection of the XVI coincides with compound X, and PG is a Boc. In this case, the removal of the protective group from the XI-XII can be carried out in conditions similar to the conversion of II to XIV and IV V.. The synthesis methods described above in schemes 1-5, also can be performed using racemic Proline derivatives or derivatives of D-Proline instead of L-Proline. So about what atom, it is possible to obtain the compounds of formula I with an alternate stereochemistry.. In the following aspect the present invention relates to pharmaceutical ...
Does LGC Biosearch Technologies synthesize minor groove binder (MGB) probes? | LGC Biosearch Technologies
Biosearch Technologies is a trusted manufacturer of custom oligos and qPCR probes for research and a GMP service provider for clinical and diagnostic markets.
Chemical and structural characterization of the interaction of bleomycin A<sub>2</sub> with d(CGCGAATTCGCG)<sub>2</sub>....
TY - JOUR. T1 - Chemical and structural characterization of the interaction of bleomycin A2 with d(CGCGAATTCGCG)2. Efficient, double-strand DNA cleavage accessible without structural reorganization. AU - Keck, M. V.. AU - Manderville, R. A.. AU - Hecht, S. M.. N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2001/9/12. Y1 - 2001/9/12. N2 - A detailed description of the interaction between Fe(II)·bleomycin A2 and the Dickerson-Drew dodecamer d(CGCGAATTCGCG)2 is presented. The reaction between bleomycin and this substrate leads to DNA cleavage at two major sites, adenosines and cytidine11, and two minor sites, cytidine3 and thymidines8. The pattern and relative intensities of cleavage at these sites was not entirely consistent with what would be predicted based on the preference of the drug for cleavage at the pyrimidines of 5′-GC-3′ and 5′-GT-3′ sites. Insight into the origins of the apparent alteration of selectivity was provided by examination of the structure ...
Heterocyclic diamidine interactions at AT base pairs in the DNA minor groove: effects of heterocycle differences, DNA AT...
Sigma-Aldrich offers abstracts and full-text articles by [Yang Liu, Catharine J Collar, Arvind Kumar, Chad E Stephens, David W Boykin, W David Wilson].
PACL case: ED attaches assets worth Rs 472 cr in Australia | Business Standard News
Read more about PACL case: ED attaches assets worth Rs 472 cr in Australia on Business Standard. The Enforcement Directorate (ED) today said it has attached assets, including shares and an immovable property, worth Rs 472 crore in Australia in connection with its money laundering probe in the PACL ponzi scam case.The central probe agency
Drug-DNA Interaction Protocols | SpringerLink
The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols
Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Re^5: Using Look-ahead and Look-behind
must match at least 50 times, so if there is AATT after say 25th character, it cannot stop there and must match a larger string. Use YAPE::Regex::Explain to see what your regular expresions mean. Moreover, you are replying to a node that is not related to your question ...
Development and comparison of a Primer-Probe Energy Transfer based assay and a 5 conjugated Minor Groove Binder assay for...
TY - JOUR. T1 - Development and comparison of a Primer-Probe Energy Transfer based assay and a 5 conjugated Minor Groove Binder assay for sensitive real-time PCR detection of infectious laryngotracheitis virus. AU - McMenamy, M.J.. AU - McKillen, J.. AU - Hjertner, B.. AU - Kiss, I.. AU - Yacoub, A.. AU - Leijon, M.. AU - Duffy, C.. AU - Belak, S.. AU - Welsh, M.. AU - Allan, Gordon. PY - 2011/8. Y1 - 2011/8. N2 - In this study the design and development of two real-time PCR assays for the rapid, sensitive and specific detection of infectious laryngotracheitis virus (ILTV) DNA is described. A Primer-Probe Energy Transfer (PriProET) assay and 5 conjugated Minor Groove Binder (MGB) method are compared and contrasted. Both have been designed to target the thymidine kinase gene of the ILTV genome. Both PriProET and MGB assays are capable of detecting 20 copies of a DNA standard per reaction and are linear from 2 x 10(8) to 2 x 10(2) copies/mu l. Neither PriProET, nor MGB reacted with heterologous ...
Distamycin - Wikipedia
Distamycin is a polyamide-antibiotic, which acts as a minor groove binder, binding to the small furrow of the double helix. Distamycin is a pyrrole-amidine antibiotic and analogous to netropsin and the class of lexitropsins. As opposed to netropsin, distamycin contains three N-methyl-pyrrole units. It is harvested from Streptomyces netropsis that also produces netropsin. Distamycin prefers AT-rich DNA-sequences and tetrades of [TGGGGT]4. Distamycin inhibits the transcription and increases the activity of the topoisomerase II. Derivates from distamycin are used as alkylating antineoplastic agents to combat tumours. Derivates with fluorophores are used as fluorescent tags for double-stranded DNA. The compound is hygroscopic, and sensible to light, freeze and hydrolysis. Its molar attenuation coefficient is 37.000 M−1 cm−1 at a wavelength of 303 nm. Lexitropsin Netropsin Hoechst 33258 DAPI M. P. Barrett, C. G. Gemmell, C. J. Suckling: Minor groove binders as anti-infective agents. In: ...
One Organic Chemist One Day: HOECHST
1956- The Company was incorporated as Hoechst Fedco Pharma Pvt. Ltd. on 31st March. The word `Private was deleted on 19th April, 1961 as the Company was deemed to be Public Limited Company under Section 41-A of the Act.- The Company manufacture bulk drugs, drug intermediates, veterinary formulations and pesticides. They established with financial and technical collaboration of Farbwerke Hoechst AG (now Hoechst AG), West Germany, which is one of the largest chemical and pharmaceutical manufacturing groups in the world.- Under the Licence and Technical Collaboration Agreement dated 11th July, made between Hoechst AG and the Company, the Company was inter alia granted the right to use the word `HOECHST in its corporate name and the use of the trade marks owned by Hoechst AG in India in respect of various pharmaceutical preparations which would be manufactured and marketed by the Company in India on the terms and conditions mentioned in the said Agreement ...
QuantiFluor-P Fluorometer Method For DNA Quantitation Using Hoechst 33258
Measure DNA using this senstive Hoechst dye-based protocol. The instrument is able to detect down to 10ng/mL with a linear range of over three logs (excitation at 350nm, emission at 450nm).
m w i -Cellstain- Hoechst 33258 solution
2) M. Sriram, van der G. A. Marel, H. L. Roelen, van J. H. Boo and A. H. Wang, Structural Consequences of a Carcinogenic Alkylation Lesion on DNA: Effect of O6-ethylguanine on the Molecular Structure of the d(CGC[e6G]AATTCGCG)-netropsin Complex, Biochemistry, 1992, 31(47), 11823. ...
Dehydroepiandrosterone supplier - Small Molecule Inhibitors of Protein Arginine Methyltransferases
mice showed a significantly increased lung fibrotic response to bleomycin compared with WT mice. cells. Analysis of hyperplastic or reactive type II alveolar epithelial cells is based on Dehydroepiandrosterone supplier the getting of (on-line methods). PGE2 from mice BAL was measured with ELISA kit from Enzo Existence Sciences (Farmingdale, NY) following a manufacturers instructions. Confocal Microscopy Immunofluorescence of freezing lungs was performed as explained (18). After main antibodies for Dehydroepiandrosterone supplier MMP19 and PTGS, samples were labeled with fluorescein isothiocyanate and Texas Red, respectively. Nuclei were counterstained with bisBenzimide Hoechst-33258. Immunohistochemistry MMP19 lung immunostaining was performed as explained using 3-amino-9-ethyl-carbazole as substrate (27). For PTGS2, the antigen-antibody complex was visualized by diaminobenzidine. Cell Microarray Lysed A549-transfected cells were labeled with the Agilent Low RNA input linear amplification Kit In ...
Drug-DNA Interactions: Structures and Spectra | Biochemistry (Chemical Biology) | Chemistry | Subjects | Wiley
Kazuo Nakamoto pioneered the use of metal isotopes to elucidate the involvement of metals in low-frequency vibrations in metallic complexes and was among the first to use matrix isolation techniques to prepare and characterize unstable specie. He is the author of Infrared and Raman Spectra of Inorganic and Coordination compounds, Fifth Edition (Wiley). Masamichi Tsuboi studies DNA and RNA structures, drug-DNA interaction, protein conformation, protein-nucleic acid recognition, and virus structure using a variety of physicochemical and biomedical techniques including X-ray diffraction, UV-Visible, IR/Raman , and NMR spectroscopy.. Gary D. Strahan uses physico-chemical techniques to understand biological systems relevant to pharmaceutical discovery and agricultural and natural product analyses. His work has emphasized spectroscopic methods, NMR, and computational modeling to determine the structures and properties of multistranded DNA, drug-DNA complexes, carbohydrates, and other biomolecules, as ...
Alliance Pharmaceutical Corp. Announces Dismissal of Arbitration Proceedings With Hoechst Marion Roussel, Inc. - Free Online...
Free Online Library: Alliance Pharmaceutical Corp. Announces Dismissal of Arbitration Proceedings With Hoechst Marion Roussel, Inc. by PR Newswire; Business News, opinion and commentary Drugs Pharmaceutical industry Licensing agreements
Methods In Molecular Biology Volume 90 Drug-DNA Interaction Protocols - ΒΙΟ-Αναγνώσεις
Introduction to Genetic Analysis 8th Edition, Anthony J.F. Griffiths, Susan R. Wessler, Richard C. Lewontin, William M. Gelbart, David T. Suzuki, Jeffrey H. Miller ...
HCS Pharma is a biotechnological startup focused on in vitro preclinical research development with a specialisation in cellular imagery : High Content Analysis (HCA) and High Content Screening (HCS ...
SPRINT==| Query Results
High mobility group (HMG)I proteins bind preferentially to the minor groove of A.T-rich regions in double-stranded DNA [1,2]. DNA-binding of these, and several related, proteins is effected by an 11-residue domain known as an A.T-hook . Within known HMG-I proteins are found three highly conserved regions, closely related to the consensus sequence TPKRPRGRPKK . A synthetic oligopeptide with this sequence specifically binds to substrate DNA in a manner reminiscent of intact HMG-I proteins. Structure predictions suggest that the peptide has a secondary structure similar to the anti-tumour and anti-viral drugs netropsin and distamycin, and to the dye Hoechst 33258 . These ligands, which also preferentially bind to A.T-rich DNA, effectively compete with both the synthetic peptide and the HMG-I proteins for DNA binding . The peptide also contains novel structural features such as a predicted Asx bend, or hook, at its N-terminus, and laterally-projecting cationic Arg/Lys bristles, which ...
Concatenar variables en sql server 2005 - Hosting Blog
This other machine can be accessed from anywhere and forward connections through the tunnel to get to the home server. The plates were incubated at 28 ВC. Then you would install SetSeed in that domain on your server. If we had to sum up InMotion in a single word, servsr would be averageв. Thats really usefull and wonderful. Flexible infrastructure, enterprise agility and accelerated breakthroughs are now concatenar variables en sql server 2005 your command. Kami menyediakan layanan infrastruktur yang siap membantu anda untuk setiap kebutuhan. Yes, on our Premium VPS products all the resources are dedicated to you. Nuclear morphology was analyzed after staining with the fluorescent dye Hoechst 33342 (Sigma-Aldrich, USA) for 10 min. From the web interface, simply select the FreeBSD ocncatenar. It perfectly blends a youthful style with elegance and luxury. FBXL20 was validated as a free web hosting rated miR-3151 target and was negatively regulated by miR-3151. Lets break this down: 10. Store ...
Effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA...
Linear and circular dichroic spectroscopies have been employed to investigate the effects of small DNA ligands on the interactions of two proteins which bind to the minor groove of DNA, viz. RecA protein from Escherichia coli and deoxyribonuclease I (bovine pancreas). Ligands representing three specific non-covalent binding modes were investigated: 4,6-diamidino-2-phenylindole and distamycin A (minor groove binders), methyl green (major groove binder), and methylene blue, ethidium bromide and ethidium dimer (intercalators). Linear dichroism was demonstrated to be an excellent detector, in real time, of DNA double-strand cleavage by deoxyribonuclease I. Ligands bound in all three modes interfered with the deoxyribonuclease I digestion of dsDNA, although the level of interference varied in a manner which could be related to the ligand binding site, the ligand charge appearing to be less important. In particular, the retardation of deoxyribonuclease I cleavage by the major groove binder methyl green
α Helix-RNA Major Groove Recognition in an HIV-1 Rev Peptide-RRE RNA Complex | Science
The solution structure of a human immunodeficiency virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of the Rev response element (RRE) RNA was solved by nuclear magnetic resonance spectroscopy. The Rev peptide has an α-helical conformation and binds in the major groove of the RNA near a purine-rich internal loop. Several arginine side chains make base-specific contacts, and an asparagine residue contacts a G·A base pair. The phosphate backbone adjacent to a G·G base pair adopts an unusual structure that allows the peptide to access a widened major groove. The structure formed by the two purine-purine base pairs of the RRE creates a distinctive binding pocket that the peptide can use for specific recognition.. ...
Plasmodium vivax: Isotopic, PicoGreen, and microscopic assays for measuring chloroquine sensitivity in fresh and cryopreserved...
In vitro susceptibility tests provide information on the intrinsic response of Plasmodium vivax to antimalarials, free from confounding factors such as host immunity or relapse. This study examined the utility of radioisotope and PicoGreen assays as alternatives to the traditional microscopic examination for assessing response of P. vivax to antimalarial drugs. There was no significant difference in the mean chloroquine IC50 of P. vivax (n = 40) as determined by the microscopic (33.4 ng/ml), isotopic (33.6 ng/ml), and PicoGreen (39.1 ng/ml) assays, respectively (F = 0.239, df = 2, 51, and p = 0.788). However measurement of IC50s by the microscopic method was slightly more successful in producing valid assays (57%), compared to the isotopic (32.5%) and PicoGreen (45.5%) methods. In a paired comparison of 20 fresh and cryopreserved isolates as examined by the microscopic method, there were no significant differences between the mean IC50 responses (T = 1.58, df = 15, and p = 0.34). Detailed ...
Dimerization of xanthorrhizol using peroxidase enzyme extracted from broccoli (Brasicca oleacea L) and its influence to the...
Xanthorrhizol, one of phytochemical compound in the extract of Curcuma xanthorrhiza Roxb, is a phenolic type compound which has abundant hydroxyl groups in its structure, thus, it is highly potential to be a hydrogen donor.
Design and synthesis of DNA minor groove methylating compounds that target pancreatic 7-cells, NC DOCKS (North Carolina Digital...
Abstract: The design of compounds that form cytotoxic, non-mutagenic 3-methyladenine adducts in pancreatic ß-cells is being studied in this project for potential applications in the treatment of diseases such as diabetes and cancer. These compounds are composed of three components: 1) a cell-targeting moiety, glucosamine, which targets the insulin producing pancreatic ß-cells by way of the GLUT-2 transporters present on these cells 2) a site-specific DNA methylating agent, Me-Lex, which has been shown to selectively produce cytotoxic, non-mutagenic N3- methyladenine adducts 3) a linker component that connects the two other components together. The linker is a critical component because it has to be such that the cell-targeting and DNAmethylating properties of the two functional components are maintained. A synthetic route was explored, which enables the easy introduction of various linkers into the molecules. Fluorescent compounds were also designed to bind weakly to DNA at the same positions ...
Splenocyte-Conditioned Media Inhibit Breast Cancer MCF-7 Cell Growth, Associated with Increased Th2/Th1 Cytokine Secretion...
Aims: To unravel the cause and possible prevention of breast cancer, five potential polysaccharides from guava seed (GSPS), common buckwheat (CBPS), bitter buckwheat (B..
Representative images and quantitative analysis of CAOV | Open-i
Representative images and quantitative analysis of CAOV-3 cells treated or not treated with liriodenine at 24 hours.Notes: The cells were stained with Hoechst,
Paid In America: The Road To The Middle | HPPR
As the presidential campaign has unfolded, the candidates have traded polemics about wealth, class warfare, taxes, dependency and the role of government.
Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by alpha helices | Science
The three-dimensional structure of a ternary complex of the purine repressor, PurR, bound to both its corepressor, hypoxanthine, and the 16-base pair purF operator site has been solved at 2.7 A resolution by x-ray crystallography. The bipartite structure of PurR consists of an amino-terminal DNA-binding domain and a larger carboxyl-terminal corepressor binding and dimerization domain that is similar to that of the bacterial periplasmic binding proteins. The DNA-binding domain contains a helix-turn-helix motif that makes base-specific contacts in the major groove of the DNA. Base contacts are also made by residues of symmetry-related alpha helices, the hinge helices, which bind deeply in the minor groove. Critical to hinge helix-minor groove binding is the intercalation of the side chains of Leu54 and its symmetry-related mate, Leu54, into the central CpG-base pair step. These residues thereby act as leucine levers to pry open the minor groove and kink the purF operator by 45 degrees. ...
The Sydney eScholarship Repository: Novel polyamide amidine anthraquinone platinum(II) complexes for enhancing DNA binding and...
A set of aminoalkylamine substituted anthraquinones (AAQs) was successfully synthesised by aminating 1- or 1,5-chloroanthraquinones in a single reaction step. These substituted anthraquinones, 1C3 or 1,5C3, were tethered to minor groove binding polyamides with between 1 - 3 N-methyl pyrrole units (Py) to produce a library of Py-AAQ compounds. It was envisaged that these compounds could improve cytotoxicity through bimodal damage to DNA relative to the individual polyamide or AAQ moieties. A set of py-AAQ platinum complexes was also successfully synthesised by a platinum-mediated alkylamine to nitrile addition. The additional conjugation of a platinum nitrile complex to Py-AAQ conjugates was predicted to generate agents that are more cytotoxic than the Py-AAQ conjugates by damaging DNA via a minor groove binding (MGB)-intercalation-platination mechanism. All newly synthesised Py-AAQs and associated imino coordinated platinum(II) complexes were examined for cytotoxic activity in DLD-1 colon and ...
SMART: AT hook domain annotation
We have determined the domains of the mammalian high mobility group (HMG)I chromosomal proteins necessary and sufficient for binding to the narrow minor groove of stretches of A.T-rich DNA. Three highly conserved regions within each of the known HMG-I proteins is closely related to the consensus sequence T-P-K-R-P-R-G-R-P-K-K. A synthetic oligopeptide corresponding to this consensus binding domain (BD) sequence specifically binds to substrate DNA in a manner similar to the intact HMG-I proteins. Molecular Corey-Pauling-Koltun model building and computer simulations employing energy minimization programs to predict structure suggest that the consensus BD peptide has a secondary structure similar to the antitumor and antiviral drugs netropsin and distamycin and to the dye Hoechst 33258. In vitro these ligands, which also preferentially bind to A.T-rich DNA, have been demonstrated to effectively compete with both the BD peptide and the HMG-I proteins for DNA binding. The BD peptide also contains ...
Top 5 Things that can sabatoge your Get Healthy Groove | HubPages
When it comes to losing your motivation, there are several sabatogers out there. Which ones have effected you, and do you know how to combat them?
Hoechst is a bis-benzimidazole derivative compound that binds to the minor groove of DNA. Often used in fluorescence microscopy ...
Latt, SA; Stetten, G (January 1976). "Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for ...
Polybenzimidazole (PBI, short for Poly-[2,2'-(m-phenylen)-5,5'-bisbenzimidazole]) fiber is a synthetic fiber with a very high ...
Molecules | Free Full-Text | Identification of Novel Bisbenzimidazole Derivatives as Anticancer Vacuolar (H+)-ATPase Inhibitors
We identified a bisbenzimidazole derivative (V) as an initial hit from a similarity search using four known V-ATPase inhibitors ... The bisbenzimidazole derivative (2e) is active against all cell lines tested. Remarkably, it demonstrated high cytotoxicity ... To the best of our knowledge the bisbenzimidazole pharmacophore has been identified as the first V-ATPase inhibitor in its ... I-IV). Based on the initial hit (V), we designed and synthesized a focused set of novel bisbenzimidazole analogs (2a-e). All ...
Molecules | Free Full-Text | Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes
The crystal of [(tbbH2)(ttcH2)2(ttcH3)(H2O)] (2) is composed of a protonated bis(benzimidazole), two ttcH2 anions, ttcH3 and ... Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes. Pavel Kopel 1,2,* , Dorota ... "Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes." Molecules 20, no. 6: 10360- ... Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes. Molecules 2015, 20, 10360- ...
1999) Bis-benzimidazole anticancer agents: Targeting human tumor helicases. Anticancer Drug Des 14:19-36. ... Transcriptional Regulation of Topoisomerase IIα at Confluence and Pharmacological Modulation of Expression by bis-Benzimidazole ... Transcriptional Regulation of Topoisomerase IIα at Confluence and Pharmacological Modulation of Expression by bis-Benzimidazole ... Transcriptional Regulation of Topoisomerase IIα at Confluence and Pharmacological Modulation of Expression by bis-Benzimidazole ...
Facile, novel two-step syntheses of benzimidazoles, bis-benzimidazoles, and bis-benzimidazole-dihydroquinoxalines<...
keywords = "Benzimidazoles, Bis-benzimidazole-dihydroquinoxalines, Bis-benzimidazoles, Multi-component reactions (MCRs), UDC", ... Facile, novel two-step syntheses of benzimidazoles, bis-benzimidazoles, and bis-benzimidazole-dihydroquinoxalines. Molecular ... Facile, novel two-step syntheses of benzimidazoles, bis-benzimidazoles, and bis-benzimidazole-dihydroquinoxalines. In: ... Facile, novel two-step syntheses of benzimidazoles, bis-benzimidazoles, and bis-benzimidazole-dihydroquinoxalines. / Xu, ...
Bis-benzimidazole derivatives as hepatitis c virus inhibitors
The present invention relates to a group of inhibiting HCV bis-benzimidazole derivatives with suitable properties for one or ... SUBSTANCE: invention refers to organic chemistry, namely to bis-benzimidazole derivatives of formula I and their optional ... This invention relates to derivatives of bis-benzimidazole, which are inhibitors of hepatitis C virus (HCV), their synthesis ... EFFECT: there are prepared bis-benzimidazole derivatives possessing the inhibitory activity on hepatitis C virus. ...
Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer...
Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer ... Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer ... Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer ... Naphthalenediimide-Linked Bisbenzimidazole Derivatives as Telomeric G‑Quadruplex-Stabilizing Ligands with Improved Anticancer ...
A Geometrically Constraining Bis(benzimidazole) Ligand and Its Nearly Tetrahedral Complexes with Fe(II) and Mn(II)
ChemIDplus - 23491-44-3 - INAAIJLSXJJHOZ-UHFFFAOYSA-N - Bisbenzimidazole - Similar structures search, synonyms, formulas,...
Stem cells and nervous tissue repair: from in vitro to in vivo
Uncoupling of S phase and mitosis induced by anticancer agents in cells lacking p21.
Drug Information Portal - U.S. National Library of Medicine - Quick Access to Quality Drug Information
Structural assembly from 1D to 3D motivated by the linear co-ligands, and the magnetic and photocatalytic properties of five...
Staining - Wikipedia
Stem Cells, Nonproliferating Cells, and Their Kinetics in Normal and Neoplastic Tissues | SpringerLink
Hoechst stain - Wikipedia
CHIMIA International Journal for Chemistry: Ingenta Connect Table Of Contents
Patente US7955889 - Organic photosensitive cells grown on rough electrode with nano-scale ... - Google Patentes
DNA Purification | DNA Extraction Methods | Promega
Zeitschrift für Naturforschung C Volume 46 Issue 7-8 (1991)
Overcoming transporter-mediated multidrug resistance in cancer: failures and achievements of the last decades | SpringerLink
Bernadou J[au] - PubMed - NCBI
Antibacterial Thiazoles | Methicillin Resistant Staphylococcus Aureus | Antimicrobial Resistance
Jennifer Frankovich | Stanford Medicine Profiles
Protocols and Video Articles Authored by Vojtech Adam
- Latt, S. A. and Stetten, G. (1976), Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. (springer.com)
- The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. (caltech.edu)
- Like bisbenzimidazole compound Hoechst 33258, these molecules also demonstrate AT-specific DNA binding. (iisc.ernet.in)
- To the best of our knowledge the bisbenzimidazole pharmacophore has been identified as the first V-ATPase inhibitor in its class. (mdpi.com)
- Agh-Atabay NM, Dulger B, Gucin F (2003) Synthesis and investigation of antimicrobial activity of some bisbenzimidazole-derived chelating agents. (springer.com)
- The synthesis and evaluation of the novel head-to-head bisbenzimidazole compound 2,2-bis[4'-(3 " -dimethylamino- 1 " -propyloxy)phenyl]-5,5-bi-1H-benzimidazole is described. (icr.ac.uk)
Designed and synthesized1
- In the present study, we have designed and synthesized three C 2 -symmetric bisubstituted bisbenzimidazole naphthalenediimide (NDI) ligands, ALI-C 3 , BBZ-ARO , and BBZ-AROCH 2 , which stabilize human telomeric G-quadruplex DNA with high affinity. (figshare.com)
- We identified a bisbenzimidazole derivative ( V ) as an initial hit from a similarity search using four known V-ATPase inhibitors ( I - IV ). (mdpi.com)