Biosensing Techniques: Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.Conductometry: Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed)Silicon: A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight [28.084; 28.086].Lab-On-A-Chip Devices: Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.Electrochemical Techniques: The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.Gold: A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.Surface Plasmon Resonance: A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.Molecular Imprinting: A methodology for chemically synthesizing polymer molds of specific molecules or recognition sites of specific molecules. Applications for molecularly imprinted polymers (MIPs) include separations, assays and biosensors, and catalysis.Streptavidin: A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.Metal Nanoparticles: Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.Transistors, Electronic: Electrical devices that are composed of semiconductor material, with at least three connections to an external electronic circuit. They are used to amplify electrical signals, detect signals, or as switches.Nanowires: Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.Semiconductors: Materials that have a limited and usually variable electrical conductivity. They are particularly useful for the production of solid-state electronic devices.Micro-Electrical-Mechanical Systems: A class of devices combining electrical and mechanical components that have at least one of the dimensions in the micrometer range (between 1 micron and 1 millimeter). They include sensors, actuators, microducts, and micropumps.Microtechnology: Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.Equipment Design: Methods of creating machines and devices.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Nanopores: Small holes of nanometer dimensions in a membrane, that can be used as single molecule detectors. The pores can be biological or synthetic.Graphite: An allotropic form of carbon that is used in pencils, as a lubricant, and in matches and explosives. It is obtained by mining and its dust can cause lung irritation.Optical Phenomena: LIGHT, it's processes and properties, and the characteristics of materials interacting with it.Electrochemistry: The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Electroplating: Coating with a metal or alloy by electrolysis.Quantum Dots: Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.Optical Devices: Products or parts of products used to detect, manipulate, or analyze light, such as LENSES, refractors, mirrors, filters, prisms, and OPTICAL FIBERS.PrintingNanotubes: Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.Equipment Failure Analysis: The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.Limit of Detection: Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.Electrodes: Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Aptamers, Nucleotide: Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.Quartz Crystal Microbalance Techniques: The use of a quartz crystal microbalance for measuring weights and forces in the micro- to nanogram range. It is used to study the chemical and mechanical properties of thin layers, such as polymer coatings and lipid membranes; and interactions between molecues.Interferometry: Measurement of distances or movements by means of the phenomena caused by the interference of two rays of light (optical interferometry) or of sound (acoustic interferometry).Enzymes, Immobilized: Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.Ion-Selective Electrodes: Electrodes which can be used to measure the concentration of particular ions in cells, tissues, or solutions.Electronics: The study, control, and application of the conduction of ELECTRICITY through gases or vacuum, or through semiconducting or conducting materials. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Avidin: A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Electricity: The physical effects involving the presence of electric charges at rest and in motion.Nanoparticles: Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.Electric Power Supplies: Devices that control the supply of electric current for running electrical equipment.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Porosity: Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.Spectrum Analysis, Raman: Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.Nanotubes, Carbon: Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.Photons: Discrete concentrations of energy, apparently massless elementary particles, that move at the speed of light. They are the unit or quantum of electromagnetic radiation. Photons are emitted when electrons move from one energy state to another. (From Hawley's Condensed Chemical Dictionary, 11th ed)Silicon Dioxide: Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.Oxides: Binary compounds of oxygen containing the anion O(2-). The anion combines with metals to form alkaline oxides and non-metals to form acidic oxides.Silver: Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Magnetics: The study of MAGNETIC PHENOMENA.Membranes, Artificial: Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.

Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. (1/3180)

Binding properties of lignin peroxidase (LiP) from the basidiomycete Phanerochaete chrysosporium against a synthetic lignin (dehydrogenated polymerizate, DHP) were studied with a resonant mirror biosensor. Among several ligninolytic enzymes, only LiP specifically binds to DHP. Kinetic analysis revealed that the binding was reversible, and that the dissociation equilibrium constant was 330 microM. The LiP-DHP interaction was controlled by the ionization group with a pKa of 5.3, strongly suggesting that a specific amino acid residue plays a role in lignin binding. A one-electron transfer from DHP to oxidized intermediates LiP compounds I and II (LiPI and LiPII) was characterized by using a stopped-flow technique, showing that binding interactions of DHP with LiPI and LiPII led to saturation kinetics. The dissociation equilibrium constants for LiPI-DHP and LiPII-DHP interactions were calculated to be 350 and 250 microM, and the first-order rate constants for electron transfer from DHP to LiPI and to LiPII were calculated to be 46 and 16 s-1, respectively. These kinetic and spectral studies strongly suggest that LiP is capable of oxidizing lignin directly at the protein surface by a long-range electron transfer process. A close look at the crystal structure suggested that LiP possesses His-239 as a possible lignin-binding site on the surface, which is linked to Asp-238. This Asp residue is hydrogen-bonded to the proximal His-176. This His-Asp...proximal-His motif would be a possible electron transfer route to oxidize polymeric lignin.  (+info)

Absorption of solar radiation by an ellipsoid sensor simulated the human body. (2/3180)

Assessment of heat gain in man caused by solar radiation is one of the most important problems in research of the human heat balance outdoors. The purpose of the present study was to investigate a new method for estimation of solar heat income. Absorption of short wave radiation (direct, diffuse and reflected) was measured with an ellipsoid sensor representing a simple, physical model of man. Measurements were performed in climatic chamber with the use of an iodide CSI solar lamp. The absorbed quantity of solar radiation varied as a result of sun altitude as well as of a colour and insulation of fabric covering the ellipsoid sensor. The new coefficients derived from our investigations for estimating doses of absorbed solar radiation should be applicable for a standing man. They correlate better with mean skin temperature observed on subjects outdoor than previous results obtained based on a cylinder as an analogue model of man. The ellipsoid sensor covered by a black fabric absorbed about 6 times more of solar radiation than when covered by a white textile.  (+info)

T cell receptor and coreceptor CD8 alphaalpha bind peptide-MHC independently and with distinct kinetics. (3/3180)

The T cell surface glycoprotein CD8 enhances T cell antigen recognition by binding to MHC class I molecules. We show that human CD8 alphaalpha binds to the MHC class I molecule HLA-A2 with an extremely low affinity (Kd approximately 0.2 mM at 37 degrees C) and with kinetics that are between 2 and 3 orders of magnitude faster than reported for T cell receptor/peptide-MHC interactions. Furthermore, CD8 alphaalpha had no detectable effect on a T cell receptor (TCR) binding to the same peptide-MHC class I complex. These binding properties provide an explanation as to why the CD8/MHC class I interaction is unable to initiate cell-cell adhesion and how it can enhance TCR recognition without interfering with its specificity.  (+info)

Qualitative and quantitative differences in T cell receptor binding of agonist and antagonist ligands. (4/3180)

The kinetics of interaction between TCR and MHC-peptide show a general relationship between affinity and the biological response, but the reported kinetic differences between antigenic and antagonistic peptides are very small. Here, we show a remarkable difference in the kinetics of TCR interactions with strong agonist ligands at 37 degrees C compared to 25 degrees C. This difference is not seen with antagonist/positive selecting ligands. The interaction at 37 degrees C shows biphasic binding kinetics best described by a model of TCR dimerization. The altered kinetics greatly increase the stability of complexes with agonist ligands, accounting for the large differences in biological response compared to other ligands. Thus, there may be an allosteric, as well as a kinetic, component to the discrimination between agonists and antagonists.  (+info)

Evaluation of relative contributions of two enzymes supposed to metabolise hydrogen peroxide in Paracoccus denitrificans. (5/3180)

A biosensor exploiting an electrochemically mediated enzyme-catalysed reaction was used to quantify relative contributions of cytoplasmic catalase and periplasmic cytochrome c peroxidase to the overall rate of hydrogen peroxide breakdown in cells of Paracoccus denitrificans. The effects of antimycin (an inhibitor of electron flow to cytochrome c peroxidase), the reaction rate versus substrate concentration profiles for the whole cells and subcellular fractions, and the time courses of oxygen concentration demonstrated a profound decrease in the capacity of cytochrome c peroxidase to reduce H2O2 under in vivo conditions. The reason is suggested to be a competition for available electrons between the enzyme and terminal oxidases metabolising oxygen produced by catalase.  (+info)

Sortilin/neurotensin receptor-3 binds and mediates degradation of lipoprotein lipase. (6/3180)

Lipoprotein lipase and the receptor-associated protein (RAP) bind to overlapping sites on the low density lipoprotein receptor-related protein/alpha2-macroglobulin receptor (LRP). We have investigated if lipoprotein lipase interacts with the RAP binding but structurally distinct receptor sortilin/neurotensin receptor-3. We show, by chemical cross-linking and surface plasmon resonance analysis, that soluble sortilin binds lipoprotein lipase with an affinity similar to that of LRP. The binding was inhibited by heparin and RAP and by the newly discovered sortilin ligand neurotensin. In 35S-labeled 3T3-L1 adipocytes treated with the cross-linker dithiobis(succinimidyl propionate), lipoprotein lipase-containing complexes were isolated by anti-sortilin antibodies. To elucidate function in cells, sortilin-negative Chinese hamster ovary cells were transfected with full-length sortilin and shown to express about 8% of the receptors on the cell surface. These cells degraded 125I-labeled lipoprotein lipase much faster than the wild-type cells. The degradation was inhibited by unlabeled lipoprotein lipase, indicating a saturable pathway, and by RAP and heparin. Moreover, inhibition by the weak base chloroquine suggested that degradation occurs in an acidic vesicle compartment. The results demonstrate that sortilin is a multifunctional receptor that binds lipoprotein lipase and, when expressed on the cell surface, mediates its endocytosis and degradation.  (+info)

Sodium dodecyl sulfate stability of HLA-DR1 complexes correlates with burial of hydrophobic residues in pocket 1. (7/3180)

Certain class II MHC-peptide complexes are resistant to SDS-induced dissociation. This property, which has been used as an in vivo as well as an in vitro peptide binding assay, is not understood at the molecular level. Here we have investigated the mechanistic basis of SDS stability of HLA-DR1 complexes by using a biosensor-based assay and SDS-PAGE with a combination of wild-type and mutant HLA-DR1 and variants of hemagglutinin peptide HA306-318. Experiments with wild-type DR1 along with previously published results establish that the SDS-stable complexes are formed only when the hydrophobic pocket 1 (P1) is occupied by a bulky aromatic (Trp, Phe, Tyr) or an aliphatic residue (Met, Ile, Val, Leu). To further explore whether the SDS sensitivity is primarily due to the exposed hydrophobic regions, we mutated residue beta Gly86 at the bottom of P1 to tyrosine, presumably reducing the depth of the pocket and the exposure of hydrophobic residues and increasing the contacts between subunits. In direct contrast to wild-type DR1, the peptide-free mutant DR1 exists as an alpha/beta heterodimer in SDS. Moreover, the presence of a smaller hydrophobic residue, such as alanine, as P1 anchor with no contribution from any other anchor is sufficient to enhance the SDS stability of the mutant complexes, demonstrating that the basis of SDS resistance may be localized to P1 interactions. The good correlation between SDS sensitivity and the exposure of hydrophobic residues provides a biochemical rationale for the use of this assay to investigate the maturation of class II molecules and the longevity of the complexes.  (+info)

Recombinant domain IV of perlecan binds to nidogens, laminin-nidogen complex, fibronectin, fibulin-2 and heparin. (8/3180)

Domain IV of mouse perlecan, which consists of 14 immunoglobulin superfamily (IG) modules, was prepared from recombinant human cell culture medium in the form of two fragments, IV-1 (IG2-9, 100 kDa) and IV-2 (IG10-15, 66 kDa). Both fragments bound to a heparin column, being eluted at ionic strengths either below (IV-2) or above (IV-1) physiological level, and could thus be readily purified. Electron microscopy demonstrated an elongated shape (20-25 nm), and folding into a native structure was indicated by immunological assay and CD spectroscopy. Solid-phase and surface plasmon resonance assays demonstrated strong binding of fragment IV-1 to fibronectin, nidogen-1, nidogen-2 and the laminin-1-nidogen-1 complex, with Kd values in the range 4-17 nM. The latter binding apparently occurs through nidogen-1, as shown by the formation of ternary complexes. Only moderate binding was observed for fibulin-2 and collagen IV and none for fibulin-1 and BM-40. Fragment IV-2 showed a more restricted pattern of binding, with only weaker binding to fibronectin and fibulin-2. None of these activities could be demonstrated for recombinant fragments corresponding to the N-terminal perlecan domains I to III. This indicates a special role for domain IV in the integration of perlecan into basement membranes and other extracellular structures via protein-protein interactions.  (+info)

*Rolling circle replication

In addition to the amplification function in biosensing applications, RCA technique can be apply to the construction of DNA ... This technique combines two fields: RCA, which allows nucleotide amplification, and immunoassay, which uses antibodies specific ... This amplification technique is named as Rolling circle amplification (RCA). Different from conventional DNA amplification ... Various derivatives of RCA were widely used in the field of biosensing. For example, RCA has been successfully used for ...

*Nanomaterial-based catalyst

Some techniques that can be used to characterize functionalized nanomaterial catalysts include X-ray photoelectron spectroscopy ... The nanozymes have found wide applications in many areas, from biosensing and bioimaging to therapeutics and water treatment. ...

*Enz-RAFT

The technique was developed at Imperial College London by Drs. Robert Chapman and Adam Gormley in the lab of Prof. Molly ... Gormley, Adam J. "Polymerization Amplified Detection for Nanoparticle-Based Biosensing". Nano Letters. 14: 6368-6373. doi: ... Enz-RAFT is a RAFT polymerization technique which allows for controlled oxygen-sensitive polymerization in an open vessel. Enz- ... With Enz-RAFT, polymerizations do not require prior degassing making this technique convenient for the preparation of most ...

*Carbon nanotube supported catalyst

Inorganic compounds are directly formed on the surface of CNTs via a variety of techniques including electrochemical techniques ... Because of the excellent biological compatibility of Au, they are so frequently used in biosensing, medical and other related ... Hydrothermal techniques are developed in recent years. The advantage of this method is to get crystalline particles or films ... The characterization techniques of CNTs supported catalysts are varied. The most common methods include X-ray diffraction (XRD ...

*Photonic metamaterial

A stacking technique for SRRs was published in 2007 that uses dielectric spacers to apply a planarization procedure to flatten ... Other potential applications include biosensing using nanoscale particles to deflect light to angles steep enough to travel ... Nanoscale fabrication techniques exist to accomplish subwavelength geometries. Metals such as gold, silver, aluminum and copper ... Fabrication techniques include electron beam lithography, nanostructuring with a focused ion beam and interference lithography ...

*Biointerface

Being a FET device can also be taken advantage of when using single SiNWs as biosensing devices. SiNW sensors are nanowires ... scientists who have developed new spectroscopic techniques to interrogate these molecules at the solid-liquid interface, and ... and regenerative medicine Computational and modeling approaches to biointerfaces Membranes and membrane-based biosensing ...

*IRIS (biosensor)

By using interferometric techniques, nanometer changes can be detected. Applications for IRIS include microarray format ... is a system that can be used as a biosensing platform capable of high-throughput multiplexing of protein-protein, protein-DNA, ...

*Nanobiotechnology

Three American patients have received whole cultured bladders with the help of doctors who use nanobiology techniques in their ... biosensing, biological mechanisms s.a. mechanosensing), nanoscience of disease (e.g. genetic disease, cancer, organ/tissue ... Measurement in biology using wave guide techniques, such as dual polarization interferometry, are another example. Applications ...

*Joseph Wang

His contributions in these directions have been of major impact in the development of electrochemical sensing techniques and ... to a novel motion-based DNA biosensing, and nanomachine-enabled isolation of biological targets, e.g. cancer-cell isolation and ... Monitoring of Environmental Pollutants in Field Electrochemistry of Nucleic Acids and Proteins Electrochemical Techniques in ...

*A. A. Griffith Medal and Prize

He is renowned for his understanding of the mechanics of materials, and for pioneering techniques that led to the study of ... Her nano-materials for biosensing have enabled the most sensitive facile enzyme detection to date and she is actively pursuing ...

*Stefan Seeger

He also developed techniques for the manipulation of biological particles. He has so far published more than 100 scientific ... 38 (4) 724-32 (1999) T. Ruckstuhl, M. Rankl, S. Seeger: Highly sensitive biosensing using a Supercritical Angle Fluorescence ( ...

*Cees Dekker

STM and nanolithography techniques were used to demonstrate that these nanotubes are quantum wires at the single-molecule level ... and resolved the mechanism of biosensing with carbon nanotubes 2008, first observation of protein-coated DNA translocation ... and discovery of a new technique for fabricating solid-state nanopores for DNA translocation 2004, discovery of new physics in ... and demonstration of an AFM technique for single-molecule manipulation of nanotubes 2001, discovery of single-electron ...

*Nanomaterials

"Refinement of the Nanoparticle Emission Assessment Technique into the Nanomaterial Exposure Assessment Technique (NEAT 2.0)". ... They are an emerging type of artificial enzyme, which have been used for wide applications in such as biosensing, bioimaging, ... There are traditional techniques developed during 20th century in Interface and Colloid Science for characterizing ... There is also a group of traditional techniques for characterizing surface charge or zeta potential of nano-particles in ...

*SensiQ

The technique provides a method for determination of the analyte diffusion coefficient and can be used to assess whether the ... Determination of kinetic/affinity interaction constants and diffusion coefficients in label-free biosensing. "Science Direct." ...

*Sweat diagnostics

... is an emerging non-invasive technique used to provide insights to the health of the human body. Common sweat ... and biosensing implications." Biomicrofluidics 9, 031301 (2015). DOI: 10.1063/1.4921039 Sato, K.; Kang, W. H.; Saga, K. & Sato ... Development of devices, sensing techniques and biomarker identification in sweat continues to be an expanding field for medical ... then the patch is analyzed utilizing techniques such as GC-MS which are accurate but the suffer the drawback in lack of ...

*Supercritical angle fluorescence microscopy

38 (4) 724-32 (1999) T. Ruckstuhl, M. Rankl, S. Seeger: Highly sensitive biosensing using a Supercritical Angle Fluorescence ( ... Supercritical angle fluorescence microscopy (SAF) is a technique to detect and characterize fluorescent species (proteins, ...

*Cadmium-free quantum dot

Through these techniques with Cd-free QDs as stable fluorescent labels, researchers can observe the cell and tissue structure ... These QDs are promising candidates as fluorescent labels in various biological applications such as bioimaging, biosensing and ... For example, various techniques are used for the Cd-free QDs imaging including confocal/multiphoton microscopy, CARS imaging. ... The ability to produce the LSPR without Cadmium is useful other labeling techniques like lateral flow immunoassay, which the ...

*Aptamer

In 1990, two labs independently developed the technique of selection: the Gold lab, using the term SELEX for their process of ... Aptamers can be used in: Affinity reagents Bioimaging probes Biosensing Therapeutics, e.g. Pegaptanib. Aptamers have an innate ... many researchers such as the Ellington lab have been developing diagnostic techniques for aptamer based plasma protein ...

*Aspergillus penicillioides

This inconsistency may be due to differences in isolation techniques. Cigar culture molded with an Aspergillus was described to ... Seo, Janghoo; Kato, Shinsuke; Tatsuma, Tetsu; Chino, Satoko; Takada, Kazutake; Notsu, Hideo (1 July 2008). "Biosensing of an ...

*Magnetic nanoparticles

Using the microemulsion technique, metallic cobalt, cobalt/platinum alloys, and gold-coated cobalt/platinum nanoparticles have ... magnetic spectroscopy and biosensing etc. Iron oxide nanoparticles Tadic, Marin; Kralj, Slavko; Jagodic, Marko; Hanzel, Darko; ... although this technique still needs further development to achieve optimal delivery to solid tumors. Another potential ...

*Self-assembling peptide

Nanotubes has also been used in the developments of electrochemical biosensing platforms and has proved to have great potential ... as the top-down method of miniaturizing device using sophisticated lithography and etching techniques has reached a physical ...

*Chromatophore

Other scientists have developed techniques for using melanophores as biosensors, and for rapid disease detection (based on the ... biomimic-based biosensing". Biosens Bioelectron. 21 (1): 111-20. doi:10.1016/j.bios.2004.08.043. PMID 15967358. Karlsson, JO; ...

*Nanomedicine

Wu, P; Yan, X. P. (2013). "Doped quantum dots for chemo/biosensing and bioimaging". Chemical Society Reviews. 42 (12): 5489-521 ... which is a clinically used technique for the purification of blood and is based on surface adsorption. These advantages are ...

*Silver nanoparticle

This technique is popular due to the ability to add a variety of functionality to the exposed silica surface. A number of ... "Metal-Enhanced Fluorescence from Silver Nanowires with High Aspect Ratio on Glass Slides for Biosensing Applications". J. Phys ... Though many different techniques for silver nanoprism synthesis exist, several methods employ a seed-mediated approach, which ... A new, very promising wet-chemical technique was found by Elsupikhe et al. (2015). They have developed a green ultrasonically- ...
Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...
Ink-jet printing has been demonstrated as a manufacturing technique that facilitates the rapid, reproducible and economical production of amperometric glucose biosensors. Glucose was chosen as the analyte for demonstrating the process. For eight electrodes produced, the relative standard deviation of the response was less than 5%. The technique is extremely versatile, and will enable a wide variety of reagents to be placed on virtually any sensor design. This technique will be of particular benefit for the mass manufacture of intricate devices, where existing production techniques, such as screen-printing, may not be suitable.. ...
A biosensor system determines analyte concentration from an output signal generated by an oxidation/reduction reaction of the analyte. The biosensor system adjusts a correlation for determining analyte concentrations from output signals at one temperature to determining analyte concentrations from output signals at other temperatures. The temperature-adjusted correlation between analyte concentrations and output signals at a reference temperature may be used to determine analyte concentrations from output signals at a sample temperature.
A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.
Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed
In recent years, there has been rapidly growing interest in developing hand held, sensitive and cost-effective on-chip biosensing systems that directly translate the presence of certain bioanalytes (e.g., biomolecules, cells and viruses) into an electronic signal. The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. All necessary sample handling and analysis steps are then performed within the chip. Microfluidic systems for biomedical analysis usually consist of a set of units, which guarantees the manipulation, detection and recognition of bioanalytes in a reliable and flexible manner. Additionally, the use of magnetic fields for performing the aforementioned tasks has been steadily gaining interest. This is because magnetic fields can be well tuned and applied either externally or from a directly integrated solution in the biosensing system. In combination with these applied magnetic
Ms. Witz led GE Healthcares Healthcare Systems Interventional global business, which researches and develops innovative medical technologies to help physicians diagnose and treat disease earlier, faster and more accurately. "Leading the GE Medical Diagnostics team is an honor," said Witz. "Im eager to continue the mission of advancing medicine through novel diagnostic agents. We will raise the bar in diagnosing critical diseases such as Parkinsons disease, Alzheimers and heart failure.". "Pascale has a breadth of experience that makes her an ideal person to lead the Medical Diagnostics business," said Dineen. "She brings an extensive background in molecular biology as well as imaging, both of which are critical to driving Medical Diagnostics development of its portfolio of innovative molecular diagnostic imaging agents.". For more information: www.gehealthcare.com. ...
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Biosensors are devices that use biological components to detect important analytes. Biosensing systems have various applications in areas such as medicine, environmental monitoring, and process control. Classical biosensors are often based on bacteria or purified enzymes that have limitations on efficiency or stability. I have developed several new biosensors to overcome these disadvantages. Two preliminary biosensors were first created based on the extremely strong and specific interaction between biotin and (strept)avidin. Both biosensors showed high sensitivity and reliability for measuring biotin with detection limits of 50-1000 pg/ml and 20-100 ng/ml, respectively. Following these, a new biosensor was developed by coupling a mobile, functionalized microsurface with cell-free expression approaches. This biosensor demonstrated a dynamic range of 1- 100 ng/ml. In addition, I also explored the possibility of combining these biosensing systems with engineered living cells. By leveraging the ...
Biosensors are poised to make a large impact in environmental, food, and biomedical applications, as they clearly offer advantages over standard analytical methods, including minimal sample preparation and handling, real-time detection, rapid detection of analytes, and the ability to be used by non-skilled personnel. Covering numerous applications of biosensors used in food and the environment, Portable Biosensing of Food Toxicants and Environmental Pollutants presents basic knowledge on biosensor technology at a postgraduate level and explores the latest advances in chemical sensor technology for researchers. By providing useful, state-of-the-art information on recent developments in biosensing devices, the book offers both newcomers and experts a roadmap to this technology. In the book, distinguished researchers from around the world show how portable and handheld nanosensors, such as dynamic DNA and protein arrays, enable rapid and accurate detection of environmental pollutants and pathogens. ...
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
Books Biofunctionalization of Nanomaterials Nanotechnologies for the Life Sciences, Vol. 1 Edited by Challa Kumar. Wiley-VCH, Weinheim 2005. 425 pp., hardcover E 139.00.-ISBN 3-527-31381-8 With this first volume, Biofunctionalization of Nanomaterials, Wiley-VCH and the editor, Challa Kumar, introduce a series that has an ambitious aim. The plan is for a ten-volume encyclopedia that will collect together and critically evaluate all aspects of the current state of knowledge about the use of nanotechnology in the life sciences. This is certainly one of the most dynamic areas of research at present, and therefore to attempt a comprehensive summary and evaluation is a challenging task. Moreover, this field has the distinction that, perhaps more than any other current area of research, it has inputs from chemistry, biology, biochemistry, materials science, and physics. Therefore, it is important that the knowledge must be communicated in a way that extends beyond the boundaries of the classical ...
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A new approach is presented to obtain fluorescent sensors for pH windows that work in water and under biomimetic conditions. A single molecule that features all-covalently linked components is used, thus making it capable of working as a fluorescent sensor with an OFF/ON/OFF response to pH value. The components are a tertiary amine, a pyridine, and a fluorophore (pyrene). The forms with both protonated bases or both neutral bases quench the pyrene fluorescence, whereas the form with the neutral pyridine and protonated amine groups is fluorescent. The molecular sensor is also equipped with a long alkyl chain to make it highly hydrophobic in all its protonated and unprotonated forms, that is, either when neutral or charged. Accordingly, it can be confined at any pH value either in traditional (i.e., low-molecular-weight) nonionic surfactant micelles or inside polymeric, biocompatible micellar containers. Relevant for future applications in vivo, thanks to its strong hydrophobicity, no leakage of ...
The aim of this work is the development of a cost effective, rapid, portable analysis system for the measurement of bioavailable toxic metal or organic contaminants, which may be present at natural attenuation landfill sites requiring remediation. The proposed system is intended to be used as a tool in the monitoring or characterisation of a site by discrete measurement. A portable system will be developed, comprising a miniaturised extraction and separation device employing capillary electrophoresis, detectors for specific analyses (metals or organics) by biosensors or immunosensors and dedicated instrumentation to process the signals generated. Development of extraction procedures for the analysis of plant material will also be part of the project, to be used when phytoremediation is employed as part of the site remediation process. Biosensors to detect toxic metal will use bioengineered proteins to interact with bioavailable metals on an electrode surface. The conformational change in the
We describe a novel biosensor system for reporting proximity between cell surface proteins in live cultured cells. The biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules. To demonstrate feasibility for the approach, two recombinant rapamycin-binding proteins were expressed as...
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Neural Networks for an Analysis of the Hemometabolites Biosensor Response: 10.4018/ijehmc.2013100106: In this work, the concentration dependent response of amperometric biosensor array for the biomarkers glucose, cholesterol and urease was explored, using
The importance of nanotechnology in medical technologies, especially biomedical diagnostics, is indubitable. By taking advantages of nanomaterials, many medical diagnostics methods have been developed so far, including electrochemical nanobiosensors. They have been used for quantification of different clinical biomarkers for detecting, screening, or follow up a disease. microRNAs (miRNAs) are one of the most recent and reliable biomarkers used for biomedical diagnosis of various diseases including different cancer types. In addition, there are many electrochemical nanobiosensors explained in publications, patents, and/or a commercial device which have been fabricated for detection or quantification of valuable miRNAs. The aim of this article is to review the concept of medical diagnostics, biosensors, electrochemical biosensors and to emphasize the role of nanotechnology in nanobiosensor development and performance for application in microRNAs detection for biomedical diagnosis. We have also summarized
The global Glucose Biosensor Market is anticipated to witness lucrative growth during the period of 2015 to 2022. The homecare diagnostics Glucose Biosensor Market is projected to flourish over the forecast period. Ease-of-use, quick results, and low costs of the tests are some aspects supporting market growth. On the other hand, the hospital-based Glucose Biosensor Market is projected to grow at a significant CAGR of 10.3% during the period 2015 to 2022.. Browse Detail Report With TOC @ http://www.hexareports.com/report/glucose-biosensor-market/details. Some key factors attributing to the growth of this market include the increasing incidence of diabetes across the globe, development of technologically advanced products, growing demand for minimally and non-invasive products, patient awareness levels, and the need for early diabetes detection. However, presence of other alternatives for management of the diabetes, such as laboratory methods is expected to restrict market growth.. Request A ...
Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...
Genetically-encoded fluorescent sensor proteins are attractive tools for studying intracellular Zn2+ homeostasis and signaling. Here we provide an overview of recently developed sensors based on Förster Resonance Energy Transfer (FRET). The pros and cons of the various sensors are discussed with respect to Z Iron and zinc sensing in cells and the body
The Nova StatStrip Glucose Hospital Meter System (StatStrip) and the StatStrip Xpress2 Glucose Hospital Meter System (StatStrip Xpress2) are the only glucose analyzers cleared by the FDA for use in all types of hospital patients, including critically ill patients receiving intensive medical intervention. For healthcare settings other than critical care, StatStrip and StatStrip Xpress2 are cleared for use with capillary finger stick, arterial, venous, neonatal heel stick and neonatal arterial specimens. When using the system to measure glucose on critically ill patients, StatStrip and StatStrip Xpress2 are cleared for use with arterial, venous, neonatal heel stick and neonatal arterial specimens. StatStrip and StatStrip Xpress2 are not cleared for capillary specimens on patients receiving intensive medical intervention/therapy because capillary samples from these patients, particularly those with decreased peripheral blood flow, may not truly reflect a patients physiological state. Some ...
Researchers at Harvard University have developed a non-toxic wearable sensor consisting of a silicon-rubber that attaches to the hand and capable of mearin | Technology
Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, "This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.". One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...
The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...
Boyce Thompson Insitute scientist Maria Harrison will collaborate with Wayne Versaw of Texas A&M University to create a new imaging system to visualize and measure the movement of phosphate from soil fungi into plant root cells in a $1.2 million DOE-funded project.
This thesis treats the development of an integrated optical sensor array. The sensors are slot-waveguide ring resonators, integrated with on-chip surface grating couplers and light splitters, for alignment tolerant, real-time, refractive index sensing, and label-free biosensing. The work includes: the design of components and system layouts, the development of fabrication methods, the fabrication of sensor chips, the characterization of the chips, and the development of physical system models for accurate extraction of resonance wavelengths in measured spectra.. The main scientific achievements include: The evaluation of a novel type of nano-structured optical waveguide for biochemical sensing. The realization of an array of such slot-waveguide sensors, integrated with microfluidic sample handling, for multiplex assays. The first study of the thermal behavior of slot-waveguide sensors and the discovery of unique temperature compensation capabilities.. From an application perspective, the use of ...
Environmental Applications of Nanoscale and Microscale Reactive Metal Particles takes the reader from current laboratory studies in the function and use of these particles through scale-up and finally to the state-of-the-science in field-scale demonstrations and applications.
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
A method for determining one member of an enzyme-substrate pair (the analyte), comprises bringing the members of the pair into contact so as to form, directly or indirectly, a soluble reaction product at or adjacent the surface of an optical waveguide biosensor. The biosensor is preferably a resonant optical biosensor based on the principle of frustrated total reflection.
A solid-state electrochemiluminescence (ECL) switch biosensor using Ru(bpy)32+/β-cyclodextrin-Pd nanoparticles (β-CY-PdNPs)/Gelatin (Gel) complex and ferrocene-labeled DNA probe (Fer-DNA) for the detection of Hg2+ was successfully developed. The ECL biosensor includes an ECL substrate and an ECL signal switc
John Fourkas, a chemistry professor at the University of Maryland, and his research team have developed materials and nanofabrication techniques to build miniaturized versions of components needed for sensors, medical diagnostics and other applications.. This approach allows for faster analysis at a lower cost with small sample volumes. The materials provide simultaneous 3D manipulation of microscopic objects using optical tweezers and a unique method for lithography, which is the process of using light in etching silicon or other substrates to create electronic components. These techniques allow complex 3D structures to be created from many microscopic components.. Previous work by this UMD research team found a breakthrough in the use of visible light for making small structures for applications including optical communications, cell behavior control and the manufacturing of integrated circuits.. Fourkas said, "One of the exciting aspects of this set of techniques is that it is compatible with ...
Title:Sol-Gel Chemistry in Biosensing Devices of Electrical Transduction: Application to CEA Cancer Biomarker. VOLUME: 15 ISSUE: 3. Author(s):Liliana A.A.N.A. Truta and M. Goreti F. Sales. Affiliation:BioMark Sensor Research, Instituto Superior de Engenharia do Porto, R. Dr. Antonio Bernardino de Almeida, 431, 4200-072 Porto, Portugal.. Keywords:Antibody, Biosensors, Cancer Biomarker, CEA, Sol-gel.. Abstract:Sol-gel chemistry allows the immobilization of organic molecules of biological origin on suibtable solid supports, permitting their integration into biosensing devices widening the possibility of local applications. The present work is an application of this principle, where the link between electrical receptor platform and the antibody acting as biorecognition element is made by sol-gel chemistry. The immunosensor design was targeted for carcinoembryonic antigen (CEA), an important biomarker for screening the colorectal cancer, by electrochemical techniques, namely electrochemical impedance ...
Sarvazyan, A.P. (1998) Mechanical Imaging A New Technology for Medical Diagnostics. International Journal of Medical Informatics, 49, 195-216.
Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen–antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via "Autodisplay". The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac ...
Biomoda granted Mexican patent for proprietary porphyrin-based compound Cancer diagnostics organization Biomoda, Inc. porphine, or TCPP, may be the foundation for many Biomoda products and medical diagnostics. Email address details are in comparison to CT Pap and scans spots examine by independent radiologists and cytopathologists, respectively, to confirm precision. Biomoda is seeking Meals and Medication Administration acceptance of its cytology-centered screening technology as a Course III medical gadget..Patients with any registration of a congenital heart defect and the ones with coronary disease diagnosed at ...
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We report on results achieved with three different types of polymer-coated chemical microsensors fabricated in industrial CMOS technology. The first and most extensively studied transducer is a microcapacitor sensitive to changes in dielectric properties of the polymer layer due to analyte absorption. An on-chip integrated (Sigma) (Delta) -converter allows for detecting the minute capacitance changes. The second transducer is a resonant cantilever sensitive to predominantly mass changes. The cantilever is electrothermally excited, its vibrations are detected using a piezoresistive Wheatstone bridge. In analogy to acoustic wave devices, analyte absorption in the polymer causes resonance frequency shifts as a consequent of changes in the vibrating mass. The last transducer is a microcalorimeter consisting of a polymer-coated sensing thermopile and an uncoated reference thermopile each on micromachined membranes. The measurand is the absorption or desorption heat of organic volatiles in the polymer ...
The potential uses of smell technology are endless," notes Josep Samitier, the coordinator of the SPOT-NOSED project that developed nanobiosensors to mimic the way human and animal noses respond to different odours. This new nose biosensor is unusual in how its made. By placing a layer of proteins that constitute the olfactory receptors in animal noses on a microelectrode and measuring the reaction when the proteins come into contact with different odorants, the system is capable of detecting odorants at concentrations that would be imperceptible to humans.. "Our tests showed that the nanobiosensors will react to a few molecules of odorant with a very high degree of accuracy. Some of the results of the trials surpassed even our expectations," Samitier says. These tiny bioelectronic sensors, he says, represent a major leap forward in smell technology and a clear example of a biomimetic devices obtained by converging Nano-Bio-Info technologies.. Several hundred different proteins, which the ...
The Biosensing program is specifically looking for highly sensitive and discriminative biosensing easy to operate sensor systems with a highly selective response to multiple analytes under variable conditions. Other needs are for cell and tissue-based sensors to monitor environmental physiological and genetic responses, smart field deployable molecular sentinels, molecular sensors capable for monitoring biological structures, and ways to combine the different sensing platforms ...
As the most common endocrine metabolic disorder for human beings, diabetes mellitus with an obvious phenomenon of high blood glucose concentrations results from a lack of insulin. Despite the availability of treatment, diabetes has remained a major cause of death and serious vascular and neuropathy diseases. Continuously monitoring the blood glucose level and intermittent injections of insulin are widely used for effective control and management of diabetes. Extensive research has been conducted to develop optimal glucose sensors for diagnostic purposes. Currently, the commercially available glucose biosensors still have some problems to overcome, such as time consuming, relatively low sensitivity, bad reliability. The performance of a glucose sensor is largely dependent upon the materials which construct the sensor. Recent research effort for glucose sensing have turned to on nanomaterials. Nanomaterial-based biosensors already have shown the capability of detecting trace amounts of biomolecules in
SAW Instruments GmbH has launched its samX biosensor for real-time, label-free assays using living cells. It provides several features that optimize t
Serum samples from CAD patients (N = 45) and healthy control subjects (N = 48) were incubated with primary human coronary artery endothelial cells at a 1:10 dilution for 4 h, followed by isolation of the cellular RNA. Alteration of inflammation-responsive elements (adhesion molecules and cytokines) was assessed by gene expression. Specific indicators included intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and interleukin-8 (IL-8). Additionally, the cytokine levels in serum samples from all subjects were quantified. Serum from CAD subjects induced greater endothelial ICAM-1, VCAM-1, and IL-8 expression compared to healthy control serum (p < 0.001 for each analysis). The three indicators of inflammatory potential (ICAM-1, VCAM-1, and IL-8 mRNA) trended independently of each other and also of serum inflammatory biomarkers. IL-8 expression correlated negatively with serum HDL levels but positively correlated with VLDL, plasminogen activator inhibitor-1 and ...
Biosensor chip, computer illustration. Biosensors are able to detect minute molecules to help monitor a persons health and their environment. Scientists are developing sensors that are sensitive enough to detect even the smallest presence of molecules, so that disease or dangerous environmental contaminants can quickly be assessed. This device consists of a tiny glass slide of sliver with protein receptors applied to it. When the receptors bind with their matching signal molecule, light waves traveling across the chip are perturbed into a recognisable interference pattern. Using this combination of light waves and biomolecules has resulted in a biochip a magnitude more sensitive than other current comparable sensors. - Stock Image C022/6578
A detection cell which is used as a component of an optical biosensor comprises a transparent base plate and a sample plate on the base plate. The sample plate has a matrix of wells extending through it to each to receive a sample. The base plate includes a waveguiding film and a diffraction grating means to in couple an incident light field into the waveguiding film beneath a well to generate a diffracted light field to enable detection of a change in the effective refractive index of the waveguiding film.
This invention describes a capacity affinity sensor based on self-assembled monolayers on an electrode with immobilized recognition elements available to analyte in the surrounding solution. Additional insulation is provided by auxiliary self-assembled molecules. The sensor has exceptional sensitivity and wide operating range due to these parts of the invention. It is versatile because different kinds of recognition elements can be immobilized directly on the surface of the measuring electrode. The electrode then becomes selective to those molecules in the solution, the analytes, that show affinity to the recognition element on the surface. Compared to capacitive sensors described before those described here shows at least a 1000-fold better sensitivity because of the properties of the layer binding the recognition element.
TY - JOUR. T1 - Label-free detection of bacterial RNA using polydiacetylene-based biochip. AU - Park, Moo Kyung. AU - Kim, Kyung Woo. AU - Ahn, Dong June. AU - Oh, Min-Kyu. PY - 2012/5/15. Y1 - 2012/5/15. N2 - We developed a simple and effective polydiacetylene-based, label-free multiplex DNA chip for the detection of various pathogenic microorganisms. A novel immobilization method of PDA vesicles on glass slides was exploited using α-cyclodextrin (α-CD). The surface topography of the efficiently immobilized PDA vesicles was confirmed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Then, oligonucleotides complementary to rRNAs of three pathogenic bacteria were conjugated to the PDA vesicles. Finally, crude lysate of pathogenic bacteria was applied to the PDA biochip. The pathogenic bacteria were specifically detected by DNA-RNA hybridization in an hour. The new PDA sensor was effective in detecting multiple pathogenic bacteria easily and accurately without rigorous ...
CROSS. The present application is a Continuation-in-Part of U.S. application Ser. No. 09/620,191, filed Jul. 20, 2000, which has issued as U.S. Pat. No. 6,488,828 on Dec. 3, 2002.. FIELD OF THE INVENTION. The present invention relates to a biosensor for use in determining the concentration of an analyte in a sample.. BACKGROUND AND SUMMARY OF THE INVENTION. Electrochemical biosensors are known. They have been used to determine the concentration of various analytes from biological samples, particularly from blood. Biosensors are described in U.S. Pat. Nos. 5,288,636; 5,413,690; 5,762,770; 5,798,031; and 5,997,817, the disclosure of each of which are hereby incorporated by reference. Storage containers for test strips are also known. See U.S. Pat. Nos. 5,788,064 and 5,985,675.. According to the present invention, a recloseable biosensor is provided that comprises a substrate formed to include a sample site, a cover including first and second ends and a middle portion between the ends, the first ...
An analytical device for predicting a subjects whole blood analyte concentration based on the subjects interstitial fluid (ISF) analyte concentration includes an ISF sampling module, an analysis module and a prediction module. The ISF sampling module is configured to sequentially extract a plurality of ISF samples from a subject. The analysis module is configured to sequentially determining an ISF analyte concentration (e.g., ISF glucose concentration) in each of the ISF samples, resulting in a series of ISF analyte concentrations. The prediction module is configured for storing the series of ISF analyte concentrations and predicting the subjects whole blood analyte concentration based on the series by performing at least one algorithm. A method for predicting a subjects whole blood analyte concentration based on the subjects interstitial fluid analyte concentration includes extracting a plurality of interstitial fluid (ISF) samples from a subject in a sequential manner and sequentially determining
Provides in-depth coverage of advances in polymeric materials used for designing biosensors and chemical sensors, including permselective membranes and immobilization for enzyme systems, electropolymerized thin films, polymer membranes on planar substrates, and hydration-dependent polymer applications. Covers fundamental aspects relevant to the design and fabrication of biosensors and offers unique insights into materials used for their fabrication.
his book, like the previous five books on biosensors by this author (and one by the co-author), addresses the neglected areas of analyte-receptor binding and dissociation kinetics occurring on biosensor surfaces.
his book, like the previous five books on biosensors by this author (and one by the co-author), addresses the neglected areas of analyte-receptor binding and dissociation kinetics occurring on biosensor surfaces.
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Use of bacteriophage T7 displayed peptides for determination of monoclonalantibody specificity and biosensor analysis of the binding reaction. ...
An implantable chemical sensor system for medical applications is described which permits selective recognition of an analyte using an expandable biocompatible sensor, such as a polymer, that undergoes a dimensional change in the presence of the analyte. The expandable polymer is incorporated into an electronic circuit component that changes its properties (e.g., frequency) when the polymer changes dimension. As the circuit changes its characteristics, an external interrogator transmits a signal transdermally to the transducer, and the concentration of the analyte is determined from the measured changes in the circuit. This invention may be used for minimally invasive monitoring of blood glucose levels in diabetic patients.
TY - JOUR. T1 - Versatile protein-a coated photoelectric immunosensors with a purple-membrane monolayer transducer fabricated by affinity-immobilization on a graphene-oxide complexed linker and by shear flow. AU - Wu, Hsueh Hsia. AU - Liao, Xin Quan. AU - Wu, Xin Ying. AU - Lin, Cheng De. AU - Jheng, Kai Ru. AU - Chen, Hong Ren. AU - Wang, Yong Yi. AU - Chen, Hsiu Mei. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Bacteriorhodopsin-embedded purple membranes (PM) have been demonstrated to be a sensitive photoelectric transducer for microbial detection. To efficiently prepare versatile BR-based immunosensors with protein A as antibody captures, a large, high-coverage, and uniformly oriented PM monolayer was fabricated on an electrode as an effective foundation for protein A conjugation through bis-NHS esters, by first affinity-coating biotinylated PM on an aminated surface using a complex of oxidized avidin and graphene oxide as the planar linker and then washing the coating with a shear flow. Three ...
Adenosine triphosphate (ATP) is an important macro molecule that is the prime supplier of energy to run cell metabolism and to regulate several physiological processes in a cell. Despite its central role, the number of non-invasive methods to study ATP on a single cell level in real time are limited. ATPases use energy derived from ATP hydrolysis to maintain cell membrane potential by regulating ion gradients across the plasma membrane. It is generally believed that the Na+/K+-ATPase (NKA) which belongs to the P-type ATPase superfamily represent the main energy consumer among the ATPases. In this study, we set out to quantify ATP consumption by NKA on a single cell level in human embryonic kidney cells (HEK293a) using PercevalHR, a genetically encoded fluorescent biosensor that reports changes in the ATP:ADP during live cell imaging. We demonstrate that ATP hydrolysis by NKA is faster at physiological temperatures (35 -37°C) compared to room temperature. K+ free KREBS pre-treatment increased ...
After obtaining a BS in Biochemistry at the University of Wisconsin-Madison, Chew went to St. Louis University to pursue his PhD, where he worked to understand the role of myosin II regulation in endothelial cells. Realizing that conventional biochemical methods could not address the spatial and temporal regulation of signaling pathways inside the cell, Chew embarked on his postdoctoral research in the laboratory of Rex Chisholm by developing fluorescent biosensor to simultaneously monitor the enzymatic activity and localization pattern of myosin light chain kinase in vivo.
The present invention relates to a novel biosensor comprising an electrode membrane combination. This novel biosensor employs compounds include a linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode, the electrode being either in part or totally made up of the linker lipid. The linker lipid comprises within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, a hydrophilic region intermediate said hydrophobic region and the attachment group and a polar head group region attached to the hydrophobic region at a site remote from the hydrophilic region.
Biosensors for small molecules can be used in applications that range from metabolic engineering to orthogonal control of transcription. Here, we produce biosensors based on a ligand-binding domain (LBD) by using a method that, in principle, can be applied to any target molecule. The LBD is fused to either a fluorescent protein or a transcriptional activator and is destabilized by mutation such that the fusion accumulates only in cells containing the target ligand. We illustrate the power of this method by developing biosensors for digoxin and progesterone. Addition of ligand to yeast, mammalian or plant cells expressing a biosensor activates transcription with a dynamic range of up to ~100-fold. We use the biosensors to improve the biotransformation of pregnenolone to progesterone in yeast and to regulate CRISPR activity in mammalian cells. This work provides a general methodology to develop biosensors for a broad range of molecules in eukaryotes.. ...
La tecnologia dels biosensors, basada en dispositius analítics que combinen un bioreceptor amb una unitat de transducció, sha aplicat en nombroses àrees de recerca per a la detecció de diferents analits dinterès. Els bacteris, especialment els bacteris patògens, són agents biològics importants per ser detectats en diversos camps com el diagnòstic clínic, la indústria alimentària o la qualitat de laigua per prevenir malalties en els éssers humans. No obstant això, els bacteris també es poden utilitzar en un ampli ventall daplicacions; per exemple, com a indicadors biològics per determinar la toxicitat de diversos compostos. En aquesta tesi es proposa lús de transductors impedimètrics basats en elèctrodes de tipus interdigitat (interdigitated electrode arrays, IDEA de les sigles en anglès) com a instrument per al desenvolupament daplicacions biosensores bacterianes. Lespectroscòpia electroquímica dimpedància és una tècnica àmpliament estudiada per caracteritzar ...
1-(beta-D-Galactopyranosyl-1-deoxy-1-iminomethyl)-2-hydroxynaphthalene (L-1), possessing an ONO binding core, was found to be selective for Cu2+ ions in N-[2-hydroxyethyl] piperazine-N-[2-ethanesulfonic acid] buffer, at concentrations ,= 580 ppb, at physiological pH by eliciting switch-on behavior, whereas the other ions, viz., Mg2+, Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Zn2+, and Cd2+, caused no significant change in the fluorescence. Whereas the binding characteristics were ascertained by absorption spectroscopy, the species formed were shown by Q-TOF ES MS ...
WASHINGTON, D.C. - Biosensor technology developed at the U.S. Naval Research Laboratory (NRL; http://stm2.nrl.navy.mil) is being incorporated in a contamination-controlled laptop veterinary diagnostic system now under development by Seahawk Biosystems Corp. (Austin, Texas.; www.seahawkbio.com).. The system is based on the NRLs Bead ARray Counter (BARC) magnetic microarray technology, and will be combined with proprietary assay protocols in hopes of greatly reducing the incidents of false positives and false negatives when testing for pathogens in pets. Seahawk says its tabletop system, scheduled to be available in 2006, will let veterinarians perform a panel of health tests simultaneously and more specifically than with current methods. The company claims that costly and time-consuming steps required to amplify or enrich the samples will be significantly reduced if not eliminated.. According to NRL, its BARC biosensor uses DNA microarrays, magnetic microbeads, and giant mangetoresistive (GMR) ...
Page contains details about glucose biosensor . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
In recent years, optical evanescent wave biosensors have been used to characterize protein‐protein interactions, including determination of equilibrium binding constants and bimolecular rate constants
The present invention provides a sensor surface comprising: a substrate coated with a free electron metal; and a matrix layer disposed on the free electron metal, wherein the matrix layer comprises an organic compound having a boronic acid complexing moiety. The matrix is preferably a self-assembled monolayer (SAM), a mixed self-assembled monolayer (mSAM), or combinations thereof.
Sautner, Éva and Papp, Krisztián and Holczer, Eszter Gabriella and Leelőssyné Tóth, Eszter and Salánki, Rita Zsanett and Szabó, Bálint and Fürjes, Péter (2017) Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system. SCIENTIFIC REPORTS, 7. p. 1008. ISSN 2045-2322 Orgovan, Norbert and Ungai-Salánki, Rita and Lukácsi, Szilvia and Sándor, Noémi and Bajtay, Zsuzsa and Erdei, Anna and Szabó, Bálint and Horváth, Róbert (2016) Adhesion kinetics of human primary monocytes, dendritic cells, and macrophages: Dynamic cell adhesion measurements with a label-free optical biosensor and their comparison with end-point assays. Biointerphases, 11. pp. 1-12. ISSN 1934-8630 Ungai-Salánki, Rita and Gerecsei, Tamás and Fürjes, Péter and Orgovan, Norbert and Sándor, Noémi and Holczer, Eszter Gabriella and Horváth, Róbert and Szabó, Bálint (2016) Automated single cell isolation from suspension with computer ...
Read user reviews, compare products and contact manufacturers of Biosensors products, including manual and automated systems, analysis software and SPR based biosensor systems on SelectScience.
A patient monitoring system measures the concentration of a particular substance in a patients tissue, blood, or other bodily fluids, provides an indication of the rate of change of such concentration, and determines whether the measured concentration and rate of change are within certain preset limits. If not, an audible and/or visual alarm signal is generated. The patient monitoring system includes at least one enzymatic sensor adapted to be inserted into the patient, where it produces sensor signals related to the concentration of the substance being measured. The sensor signals are delivered through a suitable interconnect cable to a monitor. In one embodiment, the interconnect cable includes a contactless connector that electrically isolates the enzymatic sensor from the monitor, and reduces the number of conductors required to interface with a plurality of sensors. The monitor interprets the sensor signals by applying a previously determined calibration to quantitatively determine the substance
Competing in the global economy is challenge. Market research can provide your organization with a thorough understanding of its market, customers and competitors and can help your company establish a dominant position in its industry.
President and Scientific Director, Ontario Institute for Cancer Research and Director, P3G (Public Population Project in Genomics)President and Scientific Director, Ontario Institute for Cancer Research Scientific Director, P3GDr. Thomas J. Hudson is president and scientific director of the Ontario Institute for Cancer Research. He is implementing the institutes strategic plan, working with cancer research institutions across Ontario to leverage existing strengths. The plan focuses on prevention, early diagnosis, cancer targets and new therapeutics. Its innovation platforms include imaging and interventions, bio-repositories and pathology, genomics and high-throughput screening, and informatics and biocomputing. Dr. Hudson is recruiting more than 50 internationally recognized principal investigators.Dr. Hudson was the founder and Director of the McGill University and Genome Quebec Innovation Centre and Assistant-Director of the Whitehead/MIT Center for Genome Research. Dr. Hudson is ...
This download Design of Advanced Photocatalytic Materials for Energy and was owned by an ARC Linkage International Fellowship to BG and RA, and an NSERC imaging to RA. Frisby Davis service pen institutions in back radical MH-47Es. download Design of of mini meeting with Infantry literature.
Take an in-depth look at a range of documents that show you how you can separate isomers, get cleaner spectra and remove isobaric/matrix interferences with Waters Triwave technology.
Algae are gaining wide attention in the recent times energy scenario as an alternative renewable source of biomass due to the increased awareness of finite fossil-fuel re..
The sensor system accurately detects valve stem movement, the position of the valve at any given time, with an accuracy of ± 0.1mm through the use of microchip sensors. To locate the current valve position, sensor chips inside the sensor board calculate the angle between the axial magnetic field produced by an indication pin mounted on the valve stem.. The sensor system receives signals from the PLC system to energize or de-energize the air-operated valve. It then transmits feedback signals indicating the main valve position and condition back to the PLC system.. Up to three solenoid valves convert the sensor signals from electrical current into mechanical energy to energize or de-energize the air-operated valve, using the physical stimulus of an indication pin mounted on the valve stem.. ...
Dr. Brian Cunningham has identified a novel label-free microscopy modality that quantitatively measures the scatter-induced changes in reflected intensity from a photonic crystal biosensor surface to reveal the kinetic evolution and spatial features of focal adhesions that form at the cell-surface interface. Compared to a sensing approach in which image contrast is generated by the dielectric permittivity of attached cell components, PROM provides contrast in reflected resonant intensity that is induced by the refractive index contrast of localized protein clusters that occur at the cell-surface interface that comprise FA sites. We expect PROM to be a highly useful tool that can reveal the mechanisms of biological processes that occur near the cell membrane when it is attached to extracellular matrix materials during apoptosis, stem cell differentiation, migration, division, and metastasis. ...
Some embodiments of the invention provide a single apparatus that is suitable for both spectroscopic and biosensor measurement of a fluid sample. Once the fluid is transferred to the apparatus, the apparatus can be inserted into a slot in a diagnostic measurement instrument for rapid fluid analysis. Because the apparatus is small and no pretreatment of the fluid is necessary, the diagnostic measurement instrument may be in the form of an inexpensive hand-held instrument, which could be used at the site of patient care. In some very specific embodiments, the apparatus is provided with two independent flow paths for analysis of the fluid. One flow path includes an optical chamber and the second flow path includes at least one biosensor.
Our research group is in the quest of developing a biosensor platform that is fast in response time, versatile that can be used to detect
Monty Liong is the author of this article in the Journal of Visualized Experiments: Mikroflödes On-chip Capture-cykloadditionsreaktion att reversibelt Immobilisera små molekyler eller flerkomponent Strukturer för Biosensor Applications
Medical diagnostics company Universal Biosensors, Inc. (UBI) is having a good 2015 so far, with the shares moving from 18 cents to 39 cents.. That is a far cry from the $1.80 at which UBI traded in 2010, but arguably the company has in a lot stronger shape than it was then.. Established in 2001, Universal Biosensors focuses on the development, manufacture and commercialisation of a range of in vitro point-of-care (POC) diagnostic tests. The tests come out of UBIs technology platform, which uses a novel electro-chemical cell that can give enhanced measurements in blood. The core technology is a disposable, multi-layer test strip, which uses the companys proprietary electrochemical sensors to rapidly and accurately measure bio-markers in the blood.. UBI got its first major customer in 2009 when it signed a deal with Johnson & Johnsons LifeScan subsidiary, to supply disposable blood testing strips for LifeScans One Touch Verio device, which is used by diabetics to self-monitor blood glucose ...
New pills and patches can make monitoring health as easy as checking an app on your smartphone. But could this put personal health information at risk?
An optical interferometric biosensor that rapidly and accurately detects strains of avian influenza virus has been developed by researchers to better
Methods and devices to detect analyte in body fluid are provided. Embodiments include positioning an analyte sensor in fluid contact with an analyte, detecting an attenuation in a signal from an analyte sensor after positioning during a predetermined time period, categorizing the detected attenuation in the analyte sensor signal based, at least in part, on one or more characteristics of the signal, performing signal processing to generate a reportable data associated with the detected analyte sensor signal during the predetermined time period, managing if and when to request additional reference signal measurements, and managing if and when to temporarily not display results.
Switch off the ultrasound and stow away the microscope: femtosecond laser technology has arrived. Developed by German health-tech co... Tags: cancer, femtosecond laser, optical biopsy.
Our research focuses on the development and integration of multimodal sensing and biosensing platforms both in terms of the transduction mechanism (e.g., piezoelectric, electrochemical, optical, and impedance transducers) as well as the sensing materials and interfaces with the aim to achieve higher reliability in sensing for critical real world scenarios. Such integration has significant figures of merits such as sensitivity, selectivity, and portability. Particularly, the areas of application include the biological cells based analytical systems (e.g., the cell-cell interactions, carbohydrate-protein interactions for diagnostics and therapeutics of various diseases such as cancers and bacterial infections) and also the stand-off detection of explosives and other explosive/hazardous gases (e.g., TATP, TNT, Methane, VOCs etc.). All these areas of interest and many others in the field of sensing have the potential of enhancing the quality of life, and in many cases, of saving the human lives ...
The present invention relates generally to devices and systems for detecting infection within catheters. In particular, the present invention provides catheter testing systems configured to sterilely
Description: Collection of art wherein the data processing machine contrasts data obtained from a patient having an unknown malady with data correlating to a known condition in order to make the decision ...
PSPD logo. Click to enlarge. Southwest Research Institute (SwRI) has launched a cooperative research consortium-the Particle Sensor Performance and Durability (PSPD) consortium-to investigate the performance and durability of particle sensors designed for onboard diagnostics and diesel engine emissions control. The kick-off meeting is being held today in San Antonio. Sensors...
The goal of this project is to develop a handheld biosensor for rapid detection of bioanalytes such as proteins, peptides, nucleic acids, toxins, viruses, bacteria and other molecular targets that represent interest for the biodefense area. We propose to build a rugged device, similar to a palm computer, equipped with a disposable sensor cartridge designed for simultaneous detection of multiple target molecules with sensitivity at the level of single molecule detection. Inexpensive sensor cartridge will have an array of printed receptor molecules and set of reagents for a one-step detection process. For many existing technologies, rate of response is slow, which makes them useless for biodefense applications that need results in several minutes or less. In contrast to currently available technologies that require multiple stages of reagent injection and washing, the envisioned biosensor system is based on a one-stage process that allows detection of molecular signatures in a matter of seconds or ...
A molecular sensor or chemosensor is a molecule that interacts with an analyte to produce a detectable change. Molecular sensors combine molecular recognition with some form of reporter so the presence of the guest can be observed. The term supramolecular analytical chemistry has recently been coined to describe the application of molecular sensors to analytical chemistry. Early examples of molecular sensors are crown ethers with large affinity for sodium ions but not for potassium and forms of metal detection by so-called complexones which are traditional pH indicators retrofitted with molecular groups sensitive to metals. This receptor-spacer-reporter concept is a recurring theme often with the reporter displaying photoinduced electron transfer (PET). One example is a sensor sensitive to heparin. Other receptors are sensitive not to a specific molecule but to a molecular compound class. One example is the grouped analysis of several tannic acids that accumulate in ageing Scotch whisky in oak ...
A sensor is described which utilizes the phenomenon of surface plasmon resonance to detect changes in refractive index of chemical or biochemical samples applied to a surface modified optical fiber. The sensor is constructed by polishing a short section of the lateral surface of an optical fiber to its evanescent field surrounding the fiber core. One or more thin films are applied to the polished section of the fiber to produce the sensing element. One of the films is the metal silver, which acts as the support for the surface plasmon. Under the proper conditions, TM polarized energy propagating in the fiber can be coupled to a surface plasmon electromagnetic mode on the metal film. This coupling depends on the wavelength, the nature of the fiber, the refractive index and thickness of the thin films applied to the fiber, and the refractive index of a chemical sample in contact with the modified surface. The fiber to plasmon coupling is seen as a large attenuation of the light reaching the distal ...
TY - JOUR. T1 - Development of a real-time glucose biosensor by enzyme immobilization on the quartz crystal microbalance.. AU - Lasky, S. J.. AU - Buttry, Daniel. PY - 1990/2. Y1 - 1990/2. UR - http://www.scopus.com/inward/record.url?scp=0025380012&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0025380012&partnerID=8YFLogxK. M3 - Article. C2 - 1369264. AN - SCOPUS:0025380012. VL - 8. SP - 8. EP - 16. JO - American Biotechnology Laboratory. JF - American Biotechnology Laboratory. SN - 0749-3223. IS - 2. ER - ...
An important virulence mechanism of the malaria parasite Plasmodium falciparum is cytoadhesion, the binding of infected erythrocytes to endothelial cells in the second half of asexual blood stage development. Conventional methods to investigate adhesion of infected erythrocytes are mostly performed under static conditions, many are based on manual or semi-automated read-outs and are, therefore, difficult to standardize. Quartz crystal microbalances (QCM) are sensitive to nanogram-scale changes in mass and biomechanical properties and are increasingly used in biomedical research. Here, the ability of QCM is explored to measure binding of P. falciparum-infected erythrocytes to two receptors: CD36 and chondroitin sulfate A (CSA) under flow conditions. Binding of late stage P. falciparum parasites is measured in comparison to uninfected erythrocytes to CD36- and CSA-coated quartzes by QCM observing frequency shifts. CD36-expressing cell membrane fragments and CSA polysaccharide were coated via poly-l-lysine
What is enzyme immobilization? How whole cells can be immobilized on a support? What are the advantages and disadvantages of immobilization? What are the applications of enzyme immobilization? The Supports and matrix used in enzyme immobilization technique, Different Types or methods of enzyme immobilization: Adsorption, Covalent bonding, Entrapment, Co-polymerization/cross linking and Encapsulation. Advantages and disadvantages of whole cell immobilization, Methods of whole cell immobilization. Learn more: Lecture Note in Enzyme Immobilization. You can DOWNLOAD the PPT by clicking on the download link below the preview…. ...
16. A fabrication process for fabricating a field-effect transistor device implemented on a network of vertical nanowires, including a plurality of elementary transistors, each elementary transistor comprising a source electrode and a drain electrode each positioned at one end of a vertical nanowire of the network and connected by a channel and each elementary transistor including a gate electrode surrounding each vertical nanowire of the network, the drain, source and gate electrodes of the elementary transistors implemented on the nanowires are respectively connected to each other so as to form unique drain, source and gate electrodes of the transistor device, wherein it comprises the following steps: producing source and drain electrodes at each end of each vertical nanowire, the source and drain electrodes being positioned symmetrically with respect to the gate electrode of each elementary transistor implemented on a nanowire; creating a gate electrode by depositing a layer of dielectric ...
Single-walled carbon nanotube field-effect transistors with thin tunnel barriers were investigated, and room-temperature single-electron transistor (SET) operation was realized. Single-walled carbon nanotubes were grown by thermal chemical vapor deposition at 820 degrees Celsius for 10 min using ethanol as a carbon source after forming patterned cobalt catalyst layer by conventional photolithography method. Thin tunnel barrier layer, which was oxidized 1-nm-thick aluminum layer, was inserted between nanotube channel and electrodes in order to enhance carrier confinement. Gate voltage dependences of the drain current were measured at 290 K. Clear Coulomb oscillations could be observed for the sample with tunnel barrier layer while only conventional ambipolar characteristics of conventional carbon nanotube field-effect transistors could be observed for the sample without tunnel barrier layer. These results indicate that formation of thin tunnel barrier layer is very effective for realization of SET
One-dimensional materials exhibit striking, unique phenomena that are not found in two or three dimensions. For the last twenty years, single walled carbon nanotubes (CNTs) have served as the prototypical experimental one-dimensional system. In this thesis, I investigate experimental data and theoretical models of spatially and electrically isolated single-walled CNTs field-effect transistors.; Carbon nanotubes are grown using chemical vapor deposition, which relies on small percentage of as-grown CNTs landing across pre-defined Pt electrodes that define the transistor. A Landauer model is developed, which explains the gate and bias voltage dependence of the electrical transport in these devices, and which serves as the basis for much of the analysis of the experimental electrical transport and Raman data.; Raman spectra are collected from CNTs under high applied bias voltages. When heated with an electrical current, the Raman spectra of CNTs downshift, and this shift can be used as an in-situ ...
Development of a micromachined electrode array for cochlear implant application is presented. The device is constructed from a silicon substrate with sputtered platinum electrodes and connection tracks. Electrochemical impedance spectroscopy (EIS) is used to study the properties of the electrode, and to identify potential problems caused by the micromachining process and materials. A variety of insulators are studied and a two part epoxy is identified as an adequate insulator for operation under harsh electrochemical testing conditions. The semiconducting silicon substrate is found to contribute to the total impedance of the device at high frequencies due to the thin insulating oxide between the substrate and conducting tracks. This is a potential problem for micromachined electrodes operating under high frequencies or using square stimulating pulses. The charge-delivery properties are studied using electrochemical impedance spectroscopy. It is found that platinum sputtered under particular ...

10 Semiconductors - conocimientos.com.ve: Organic semiconductor10 Semiconductors - conocimientos.com.ve: Organic semiconductor

The doctor-blade technique has a minimal material loss and was primarily developed for large area thin film production. Vacuum ... electrochemical transistors and recently in biosensing applications. Organic semiconductors have many advantages, such as easy ... "Wet" coating techniques require polymers to be dissolved in a volatile solvent, filtered and deposited onto a substrate. Common ... Wet coating techniques can be applied to small molecules but to a lesser extent depending on material solubility. ...
more infohttp://conocimientossemiconductors.blogspot.com/2010/03/organic-semiconductor.html

WikiGenes - Biosensing TechniquesWikiGenes - Biosensing Techniques

High impact information on Biosensing Techniques. *Using a FRET biosensor, we find that LGN behaves as a conformational switch ... Psychiatry related information on Biosensing Techniques. *The biosensor operated at 0.450 V, had a fast response time (t90% , ... Associations of Biosensing Techniques with chemical compounds. *Using genetically targeted PIP2-sensitive inward rectifier ... Gene context of Biosensing Techniques. *Using an optical biosensor, we found that CD4-induced (CD4i) epitopes recognized by ...
more infohttps://www.wikigenes.org/e/mesh/e/4661.html

Research Keyword Faculty Listing | Yale School of MedicineResearch Keyword Faculty Listing | Yale School of Medicine

Biosensing Techniques. Chenxiang Lin, PhD Associate Professor of Cell Biology. Research Interests. Biophysics; Biosensing ... Techniques; Cell Biology; Cell Membrane; DNA; Membrane Fusion; Membranes, Artificial; Nanostructures; Nanotechnology; Nuclear ...
more infohttps://medicine.yale.edu/research/listing.aspx?meshId=14698

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination | IntechOpenDynamic Luminescent Biosensors Based on Peptides for Oxygen Determination | IntechOpen

This chapter summarizes the fundamentals of biosensing techniques based on fluorescence spectroscopy and the protagonism of ... 2. Principles and evolution of biosensing techniques. Biosensors are devices that can perform the measurement of a ... This chapter summarizes the fundamentals of biosensing techniques based on fluorescence spectroscopy and the protagonism of ... In biosensing, materials based on graphene had promoted efficient detection of biomarkers and have proportioned an important ...
more infohttps://www.intechopen.com/books/biosensors-for-environmental-monitoring/dynamic-luminescent-biosensors-based-on-peptides-for-oxygen-determination

Characterization of a gold coated cantilever surface for biosensing applications | EPJ Techniques and Instrumentation | Full...Characterization of a gold coated cantilever surface for biosensing applications | EPJ Techniques and Instrumentation | Full...

EPJ Techniques and Instrumentationvolume 2, Article number: 1 (2015) , Download Citation ... Characterization of a gold coated cantilever surface for biosensing applications. *Ann-Lauriene Haag1. , ... allowing signal averaging techniques to be used. If clean gold surfaces are used, the resultant reproducible time dependent ... different cleaning procedures by quantifying surface cleanliness electrochemically in situ and using surface science techniques ...
more infohttps://epjtechniquesandinstrumentation.springeropen.com/articles/10.1140/epjti/s40485-014-0011-5

Predicting aggression to others in youth with autism using a wearable biosensor.  - PubMed - NCBIPredicting aggression to others in youth with autism using a wearable biosensor. - PubMed - NCBI

aggression; autism spectrum disorder; autonomic nervous system; biosensing techniques; inpatients. PMID:. 31225952. DOI:. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/31225952

Category:Biotechnology - Wikimedia CommonsCategory:Biotechnology - Wikimedia Commons

Biosensing techniques‎ (35 F). *. ► Biotechnology databases‎ (1 C). C. *. ► Cell culture techniques‎ (5 C, 274 F) ...
more infohttps://commons.wikimedia.org/wiki/Category:Biotechnology

Category:Biotechnology - Wikimedia CommonsCategory:Biotechnology - Wikimedia Commons

Biosensing techniques‎ (35 Ն). *. ► Biotechnology databases‎ (1 Կ). C. *. ► Cell culture techniques‎ (5 Կ, 275 Ն) ...
more infohttps://commons.wikimedia.org/wiki/Category:Biotechnology?uselang=hy

David N. Beratan, R.J. Reynolds Professor of ChemistryDavid N. Beratan, R.J. Reynolds Professor of Chemistry

bioenergetics • Biophysics • Biosensing Techniques • Chemistry, Physical • chemistry, physical and theoretical • Chemistry, ...
more infohttp://fds.duke.edu/db/pratt/FIP/david.beratan

International Journal of Signal and Imaging Systems Engineering (IJSISE)  Inderscience Publishers - linking academia, business...International Journal of Signal and Imaging Systems Engineering (IJSISE) Inderscience Publishers - linking academia, business...

Optical biosensing techniques. *External Reflection techniques, backscattering multispectral polarimetry. *Total internal ... Linear and Nonlinear Techniques for Image Processing. *Advanced image enhancement and processing algorithms ... Image System Design, Image Instrumentation and Measuring Techniques. *Image system design parameters, such as spectral response ... IJSISE provides the interface between basic research, algorithms and techniques in signal and image processing/analysis/ ...
more infohttps://www.inderscience.com/jhome.php?jcode=ijsise

Bioprobes - Semantic ScholarBioprobes - Semantic Scholar

Specific bioprobes with fluorescence turn-on response are highly desirable for high contrast biosensing and imaging. In this ...
more infohttps://www.semanticscholar.org/topic/Bioprobes/1037894

Measured (marks) and calculated sensitivities (lines) f | Open-iMeasured (marks) and calculated sensitivities (lines) f | Open-i

Biosensing Techniques/instrumentation*. *Refractometry/instrumentation*. Minor. *Models, Theoretical. *Optical Devices. * ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4493545_biosensors-05-00187-g007&req=4

Sensitivity of peroxalate micelles to hydrogen peroxide | Open-iSensitivity of peroxalate micelles to hydrogen peroxide | Open-i

Biosensing Techniques/methods*. *Drug Carriers/chemistry*. *Hydrogen Peroxide/analysis*/chemistry*. *Luminescent Measurements/ ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2636589_ijn-3-471f3&req=4

Stephanie Carreiros research topics | Profiles RNSStephanie Carreiro's research topics | Profiles RNS

Biosensing Techniques. To see the data from this visualization as text, click here. ...
more infohttps://profiles.umassmed.edu/display/132615/network/researchareas/timeline

Woodhead Publishing - Medical Biosensors for Point of Care (POC) Applications - Roger J Narayan - 9780081000786 - Schweitzer...Woodhead Publishing - Medical Biosensors for Point of Care (POC) Applications - Roger J Narayan - 9780081000786 - Schweitzer...

... materials and fabrication of medical biosensors while the next part looks at different technologies and operational techniques ... 7.2.1 Biosensing techniques. *7.2.2 Readout circuits. *7.2.3 Design example of pH sensor and readout circuit ... materials and fabrication of medical biosensors while the next part looks at different technologies and operational techniques ...
more infohttps://www.schweitzer-online.de/ebook/Narayan/Medical-Biosensors-for-Point-Care-POC-Applications/9780081000786/A37835649/

Upcoming Special IssuesUpcoming Special Issues

Special issue entitled: Biosensing techniques for cancer biomarkers is being edited by * Biography ...
more infohttps://www.longdom.org/biochemistry-and-analytical-biochemistry/upcoming-special-issues.html

Department of Pediatrics - Research Output
     - Penn StateDepartment of Pediatrics - Research Output - Penn State

Bateman, S. T., Lacroix, J., Boven, K., Forbes, P., Barton, R., Thomas, N. J., Jacobs, B., Markovitz, B., Hanson, J. H., Li, H. A., Abd-Allah, S., Randolph, A., Healey, H., Besunder, J., Blumer, J., Cheiftez, I., Bass, A., Dalton, H., Dobyns, E., Fitch, J. & 21 others, Gedeit, R., Goldstein, B., Grzeszczak, M., Hasan, R., Jefferson, L., Levin, D., Jarvis, D., Newth, C., Randolph, A. G., Auw, L., Patel, V., Ron, S., Correa, C. S., Seferian, E., Hess, J., Stokes, J., Willson, D., Cox, P., Joffe, A., Menon, K. & Wensley, D., Jul 1 2008, In : American journal of respiratory and critical care medicine. 178, 1, p. 26-33 8 p.. Research output: Contribution to journal › Article ...
more infohttps://pennstate.pure.elsevier.com/en/organisations/department-of-pediatrics-2/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&page=6&ordering=type&descending=false

Biomedical Engineering - Research Output
     - Penn StateBiomedical Engineering - Research Output - Penn State

Impact of Translational Research on Optimization of Neonatal Cardiopulmonary Bypass Circuits and Techniques-The Penn State ...
more infohttps://pennstate.pure.elsevier.com/en/organisations/biomedical-engineering/publications/?page=6

Surface Plasmon Resonance | Profiles RNSSurface Plasmon Resonance | Profiles RNS

A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one ... The technique is used to study biomolecular interactions, such as antigen-antibody binding. ...
more infohttps://profiles.umassmed.edu/display/128361

Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation - Analyst...Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation - Analyst...

They are decorated with surface proteins, which are membrane proteins that can be targeted by biochemical techniques to isolate ... Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation ... Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation M. ... They are decorated with surface proteins, which are membrane proteins that can be targeted by biochemical techniques to isolate ...
more infohttps://pubs.rsc.org/en/content/articlelanding/2020/an/c9an01949a/unauth
  • A time-domain-based amplifier concept is proposed to obtain high-voltage gains with low power consumption and at an ultra-low supply voltage of 0.35 V. A prototype instrumentation amplifier designed using the proposed technique in the 180 nm technology consumes 210 nW of power and 0.1 mm 2 of the active area. (digchip.com)
  • These advantages make the fluorescence spectroscopy a powerful technique, paving the way to the most wished rapid and low-cost sensing in a wide range of biological and environmental applications and point-of-care diagnostics for real-time monitoring of physiological conditions. (intechopen.com)
  • Fluorescence spectroscopy has been revolutionizing the field of life sciences and clinical routines such as diagnostics and biosensing, due to its impressive sensitivity and the biocompatibility of many fluorescent organic compounds, which allows one to probe biological processes in vivo in noninvasive bioimaging procedures. (intechopen.com)