A huge subclass of mostly marine CRUSTACEA, containing over 14,000 species. The 10 orders comprise both planktonic and benthic organisms, and include both free-living and parasitic forms. Planktonic copepods form the principle link between PHYTOPLANKTON and the higher trophic levels of the marine food chains.
A genus of gram-negative bacteria existing symbiotically with nematodes of the family Heterorhabditidae (see RHABDITOIDEA). These nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases Photorhabdus from its intestinal tract and the bacterium establishes a lethal septicemia in the insect.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Molecular Probe Techniques
Green Fluorescent Proteins
Recombinant Fusion Proteins
Fluorescence Resonance Energy Transfer
Encyclopedias as Topic
Promoter Regions, Genetic
Retinal Artery Occlusion
Portraits as Topic
Education, Pharmacy, Graduate
A historical and cultural entity dispersed across a wide geographical area under the influence of Greek civilization, culture, and science. The Greek Empire extended from the Greek mainland and the Aegean islands from the 16th century B.C., to the Indus Valley in the 4th century under Alexander the Great, and to southern Italy and Sicily. Greek medicine began with Homeric and Aesculapian medicine and continued unbroken to Hippocrates (480-355 B.C.). The classic period of Greek medicine was 460-136 B.C. and the Graeco-Roman period, 156 B.C.-576 A.D. (From A. Castiglioni, A History of Medicine, 2d ed; from F. H. Garrison, An Introduction to the History of Medicine, 4th ed)
Stem Cell Transplantation
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Embryonic Stem Cells
Förster resonance energy transfer
This technique uses a bioluminescent luciferase (typically the luciferase from Renilla reniformis) rather than CFP to produce ... This luciferase is smaller (19 kD) and brighter than the more commonly used luciferase from Renilla reniformis., and has been ... November 2012). "Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate". ACS ... BRET has also been implemented using a different luciferase enzyme, engineered from the deep-sea shrimp Oplophorus ...
The sea pansy is strikingly bioluminescent when disturbed because of the interplay between a luciferase (Renilla-luciferin 2- ... The sea pansy, Renilla reniformis, is a species of colonial cnidarian in the family Renillidae, part of an octocoral subclass ... "Sea Pansy (Renilla reniformis)". Encyclopedia of Life. Retrieved 10 June 2014. Ruppert E, Fox R (1988). Seashore Animals of the ... "Antipredator defense and phenotypic plasticity of sclerites from Renilla muelleri, a tropical sea pansy". The Biological ...
... , Renilla luciferase, or RLuc, is a bioluminescent enzyme found in Renilla reniformis, ... belonging to a group of coelenterazine luciferases. Of this group of enzymes, the luciferase from Renilla reniformis has been ... Renilla luciferase contains 311 amino acids, and is active as a nearly spherical single polypeptide chain monomer of 36 kDa, ... Loening AM, Fenn TD, Wu AM, Gambhir SS (September 2006). "Consensus guided mutagenesis of Renilla luciferase yields enhanced ...
... such as Renilla and Gaussia luciferases, it does not share base pair sequences with these enzymes [3,4,5,7]. OpLuc catalyzes ... The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine. Biochemical ... It is the third example of a luciferase (Other than Aequorea and Renilla) to be purified in lab . The systematic name of ... Although originally thought to have the exact same mechanism as the Renilla luciferase , this luminescence has two possible ...
... of this emission makes detection of firefly luciferase much more sensitive in vivo. Renilla luciferase (from the Sea pansy) ... "Autonomous Bioluminescent Expression of the Bacterial Luciferase Gene Cassette (lux) in a Mammalian Cell Line". PLOS ONE. 5 (8 ... using a bioluminescent cancer cell line), and reconstitution kinetics (using bioluminescent stem cells). Researchers at UT ... "Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of ...
A click-beetle luciferase is also available that produces light at a peak closer to 595 nm. Both luciferases require the ... Variants have also been isolated from the sea pansy Renilla reniformis. GFP, like aequorin, produces a blue fluorescent signal ... Depending on the combination of these genes used, several different types of bioluminescent bioreporters can be constructed. ... Luciferase is a generic name for an enzyme that catalyzes a light-emitting reaction. Luciferases can be found in bacteria, ...
Also well studied is the sea pansy, Renilla reniformis. In this organism, the luciferase (Renilla-luciferin 2-monooxygenase) is ... The opportunities for using luciferase continue to expand. Luciferin Bioluminescence imaging Quorum sensing Bioluminescent ... Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be ... In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can ...
Förster resonance energy transfer
This technique uses a bioluminescent luciferase (typically the luciferase from Renilla reniformis) rather than CFP to produce ... This luciferase is smaller (19 kD) and brighter than the more commonly used luciferase from Renilla reniformis. ... One drawback of BRET is the requirement to generate at least one fusion protein encoding luciferase, though some applications ... BRET has also been implemented using a different luciferase enzyme, engineered from the deep-sea shrimp Oplophorus ...
... a 2020 study found a potential endogenous pyrosome luciferase in the organism's transcriptome homologous to Renilla luciferase ... P. atlanticum is bioluminescent and can generate a brilliant blue-green light when stimulated. P. atlanticum is found in ... RLuc). Further study of the luciferase showed that it reacted with coelenterazine to produce light, much like RLuc. Five ... "A putative chordate luciferase from a cosmopolitan tunicate indicates convergent bioluminescence evolution across phyla". ...
It is the substrate of many luciferases such as Renilla reniformis luciferase (Rluc), Gaussia luciferase (Gluc), and ... A novel secreted bioluminescent reporter enzyme". The Journal of Biological Chemistry. 279 (5): 3212-7. doi:10.1074/jbc. ... Markova SV, Golz S, Frank LA, Kalthof B, Vysotski ES (January 2004). "Cloning and expression of cDNA for a luciferase from the ... Many members of the genus Metridia also produce luciferases that use this compound, some of which are secreted into ...
Pierce Renilla Luciferase Flash Assay Kit - Thermo Fisher Scientific
Features of the Renilla Luciferase Flash Assay Kit: Sensitivegreater sensitivity all ... The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular ... When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter ... The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea ...
Thermo Scientific™ Green Renilla Luciferase Reporter Assay Vectors
... that drives green Renilla luciferase expression as a reporter for gene regulation studies. ... Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple ... pMCS-Green Renilla Luc Vector. Drive Green Renilla luciferase expression as a reporter for gene regulation studies using ... Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems ...
Designing a Bioluminescent Reporter Assay: Choosing Your Experimental Reporter
Luciferase Assay System Reagent for firefly luciferase and Renilla-Glo™ Luciferase Assay System Reagent for Renilla luciferase. ... We use three luciferases as reporters in our bioluminescent assays: NanoLuc® Luciferase (19kDa), Renilla Luciferase (36kDa) and ... Firefly and Renilla luciferases can be used together with the Dual-Luciferase® (flash kinetics) or Dual-Glo® (stable kinetics) ... NanoLuc® luciferase was approximately 150-fold brighter than firefly or Renilla luciferases at equivalent concentrations. ...
Highly sensitive dual luciferase detection with the SpectraMax DuoLuc Reporter Assay Kit | Molecular Devices
Learn how dual luciferase detection with SpectraMax DuoLuc Reporter Assay Kit enables highly sensitive quantitation of firefly ... Figure 1. Bioluminescent reactions catalyzed by (A) firefly luciferase and (B) Renilla luciferase ... Highly sensitive dual luciferase detection with the SpectraMax DuoLuc Reporter Assay Kit *Detect firefly and Renilla luciferase ... pGL4.13[luc2/SV40] firefly luciferase expression vector (Promega cat. #E668A). *pGL4.75[hRluc/CMV] Renilla luciferase ...
Renilla-luciferin 2-monooxygenase - Wikipedia
Renilla-luciferin 2-monooxygenase, Renilla luciferase, or RLuc, is a bioluminescent enzyme found in Renilla reniformis, ... belonging to a group of coelenterazine luciferases. Of this group of enzymes, the luciferase from Renilla reniformis has been ... Renilla luciferase contains 311 amino acids, and is active as a nearly spherical single polypeptide chain monomer of 36 kDa, ... Cormier, Milton J.; Karkhanis, Yashwant D. (January 1971). "Isolation and properties of Renilla reniformis luciferase, a low ...
Bioluminescent Endoscopic Imaging: Technical Requirements of the Chemistry and Camera System - SAGES Abstract Archives
The chemistry of the renilla luciferase, gaussia luciferase and coelentrazine (BioLume, Inc.) is described. The range and ... In this study we describe and define the chemistry of the bioluminescent coelenterazine-luciferase system and the technical ... Bioluminescent Endoscopic Imaging: Technical Requirements of the Chemistry and Camera System. Objective: Bioluminescent ... Conclusions: Endoscopic bioluminescent imaging is a completely new way of instantly and directly imaging precarious anatomy in ...
Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.Features of the Cypridina-Firefly Luciferase Dual ... The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously ... Cypridina luciferase produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent ... Red firefly luciferase is a mutant form of the Japanese firefly luciferase from Luciola cruciata. This luciferase produces a ...
Designing a Bioluminescent Reporter Assay-Part 1-Choosing Your Experimental Reporter
... offers several reporter and assay combinations for different situations.This article helps you choose the bioluminescent ... We use three luciferases as reporters in our bioluminescent assays: NanoLuc® Luciferase (19kDa), Renilla Luciferase (36kDa) and ... Firefly and Renilla luciferases can be used together with the Dual-Luciferase (flash kinetics) or Dual-Glo (stable kinetics) ... On a per mole basis, NanoLuc® Luciferase is approximately 100-fold brighter than Firefly or Renilla Luciferase. This difference ...
Jianghong Rao | Stanford Medicine Profiles
This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins ... Bioluminescent nanosensors for protease detection based upon gold nanoparticle-luciferase conjugates CHEMICAL COMMUNICATIONS ... The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the ... This protocol describes the preparation of Renilla luciferase (rLuc) with 1,2-aminothiol at either its N- or C-terminus, and ...
Andreas Loening | Stanford Medicine Profiles
... by coupling carboxylate-presenting quantum dots to a mutant of the bioluminescent protein Renilla reniformis luciferase. We ... firefly luciferase (fluc) and Renilla luciferase (hrluc), driven by the cardiac troponin T (cTnT) promoter. The vector was ... Red-shifted Renilla reniformis luciferase variants for imaging in living subjects NATURE METHODS Loening, A. M., Wu, A. M., ... A red-shifted Renilla luciferase for transient reporter-gene expression NATURE METHODS Loening, A. M., Dragulescu-Andrasi, A., ...
Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity | The Journal of Immunology
Renilla, and bacterial luciferases (16). Bioluminescent reporter systems have previously been developed for some other ... Bioluminescent Reporting of In Vivo IFN-γ Immune Responses during Infection and Autoimmunity. Catherine J. Reynolds, Deborah L ... Bioluminescent imaging of IFN-γ offers an approach to questions of comparative screening of T cell immune responses, whether ... an IFN-γ reporter mouse in which IFN-γ production can be visualized in vivo as a bioluminescent signal from firefly luciferase ...
Luminescent Probes and Visualization of Bioluminescence | SpringerLink
Renilla, Gaussia, and Cypridina luciferases, and calcium-activated photoproteins. According to the specific bioluminescent ... Bioluminescent proteins Photinus pyralis luciferase bacterial luciferase Gaussia luciferase aequorin reporter gene ... Following ohe discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases ... Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, ...
Bioluminescence and Fluorescence for In Vivo Imaging | (2010) | Brovko | Publications | Spie
4.1 Luciferase-Based Reporters of Gene Expression. 4.1.1 Bioluminescent gene reporters for clinical research. 4.1.2 ... 3.1.2 Viability/Proliferation tests based on bacterial luciferase. 3.1.3 Viability/Proliferation tests based on Renilla and ... 1.2 Bioluminescent System of Bacteria. 1.3 Bioluminescent System of Insects (Fireflies). 1.4 Bioluminescent System of Ostracods ... 5.1.1 Bioluminescent in vivo ATP imaging. 5.1.2 Fluorescent in vivo ATP imaging. 5.2 Bioluminescent In Vivo Imaging of Calcium ...
JCI - ΔNp63-driven recruitment of myeloid-derived suppressor cells promotes metastasis in triple-negative breast cancer
... and Renilla luciferase to facilitate in vivo tracking (33). Isoform-specific knockdown (KD) of ΔNp63 was confirmed at both the ... Representative mouse images (left) and tumor growth (right; total flux, photons per second [p/s]) show bioluminescent signal ... and Renilla luciferase-expressing control and ΔNp63-KD HCC1806 cells (2 × 105) were injected into the mammary fat pad of nude ... Bioluminescence imaging of tumor and lung tissues. Mice were anesthetized and injected with 100 μl of Renilla luciferin ...
Förster resonance energy transfer - Wikipedia
This technique uses a bioluminescent luciferase (typically the luciferase from Renilla reniformis) rather than CFP to produce ... This luciferase is smaller (19 kD) and brighter than the more commonly used luciferase from Renilla reniformis., and has been ... November 2012). "Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate". ACS ... BRET has also been implemented using a different luciferase enzyme, engineered from the deep-sea shrimp Oplophorus ...
Molecular Imaging of Stem Cells - IOS Press
... firefly luciferase, Rluc: renilla luciferase, NIS: sodium iodine symporter, HSV1-tk: herpes simplex virus type 1 thymidine ... the bioluminescent (firefly luciferase, Fluc) and PET reporter genes (herpes simplex virus enzyme thymidine kinase, HSV1-tk) ... Loening AM , Wu AM , Gambhir SS . Red-shifted Renilla reniformis luciferase variants for imaging in living subjects. Nat ... Loening AM , Fenn TD , Wu AM , Gambhir SS . Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and ...
... bioluminescent and colorimetric change reporter genes for your cell culture and in vivo experiments. See more here! ... For bioluminescent imaging, we offer reporter genes luciferase, firefly luciferase (FLuc) and Renilla luciferase mRNAs. In the ... Bioluminescent reporters are useful for time-course studies in cells and in vivo. For example, β-galactosides (β-gal) mRNA can ... presence of luciferin or coelenterazine, respectively, the bioluminescent proteins expressed from these mRNAs release visible ...
Methods of Analysis for Imaging and Detecting Ions and Molecules - The Chemical Society of Japan
The principle is based on reconstitution of split fragments of Renilla luciferase (Rluc) by protein splicing with a DnaE intein ... His approach for this has been to develop genetically encoded fluorescent and bioluminescent indicators to pinpoint each ... Umezawas group developed a genetically encoded bioluminescent indicator for monitoring the nuclear trafficking of target ... signaling can be monitored in vivo in living cells by genetically encoded intracellular fluorescent and bioluminescent probes ...
File S2: All Luc Like (fasta format) from A puzzling homology: a brittle star using a putative cnidarian-type luciferase for...
... a brittle star using a putative cnidarian-type luciferase for bioluminescence ... luciferases allowed us to detect the latter using anti-,i,Renilla,/i, luciferase antibodies. Luciferase expression was ... specifically localized in the spines which were demonstrated to be the bioluminescent organs ,i,in vivo,/i,. However, enzymes ... Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases ...
Preventing tumor escape by targeting a post-proteasomal trimming independent epitope | JEM
Fluorescent proteins - Scholarpedia
In bioluminescent organisms such as Aequorea and Renilla, fluorescent proteins are found to be partnered with the ... 1971) and luciferase (Ward and Cormier 1976), respectively. The function of the fluorescent protein is to act as a ... Ward, W.W. and Cormier, M.J. (1976) In vitro energy transfer in Renilla bioluminescence, J. Phys. Chem., 80, 2289-2291.. ... Discovery of Renilla green fluorescent protein by John E. Wampler. A discussion of coral fluorescence for aquarium hobbyists by ...
Team:Heidelberg/Notebook/Methods - 2010.igem.org
... and Renilla (Renilla reniformis) luciferases (RL) are measured sequentially from a single sample. Firefly and Renilla ... This allows for selective discrimination between their bioluminescent reactions. The firefly luciferase (FL) reporter is ... Dual Luciferase Assay We measured the knockdown of firefly luciferase using the Promega Dual Luciferase Reporter Assay. The DLR ... which is able to quench the firefly luciferase activity and simultaneously contains the substrate for Renilla luciferase, ...
JCI - Chaperone protein HSC70 regulates intercellular transfer of Y chromosome antigen DBY
Marilyn-BLITC mice are T cell receptor transgenic mice expressing Renilla luciferase (Rluc) and the NFAT-dependent click-beetle ... Bioluminescent imaging. In vivo imaging was performed using a Xenogen IVIS 200 (Caliper Lifescience). In order to analyze ... A transgenic dual-luciferase reporter mouse for longitudinal and functional monitoring of T cells. Cancer Immunol Res. 2018;6(1 ... Renilla luciferase (Rluc) signals for T cell migration/expansion, anesthetized mice were imaged 3 minutes after intravenous ...
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Renilla Luciferase, breast cancer, metastasis, Smad. 3245. Play Button. Bioluminescent Orthotopic Model of Pancreatic Cancer ... Murine Bioluminescent Hepatic Tumour Model. Authors: Simon Rajendran, Slawomir Salwa, Xuefeng Gao, Sabin Tabirca, Deirdre ... Luciferase-tagged pancreatic cancer cells are resuspended in Matrigel and delivered into the pancreatic tail during laparotomy ... Tumour cell death and necrosis due to drug treatment is indicated early by a reduction in the bioluminescent signal. This mouse ...
Binding Sites in the EFG1 Promoter for Transcription Factors in a Proposed Regulatory Network: A Functional Analysis in the...
1996 The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression ... OpTSP, opaque transcription start point; RLUC, Renilla reniformis luciferase; WhTSP, white transcription start point; WT, wild ... In vitro luciferase assay. Luciferase activity was assayed according to methods previously described in detail (Srikantha et al ... promoter were placed upstream of the Renilla luciferase gene RLUC, and the constructs inserted downstream of either one of the ...
Frontiers | Pivotal Role of Adenosine Neurotransmission in Restless Legs Syndrome | Neuroscience
Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in...
We have studied four codon optimized luciferases with different emission spectra, … ... In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation ... and Renilla reniformins (hRLuc). At 25 degrees C, the in vitro lambda(max) of these reporters are 578, 543, 615, and 480 nm, ... Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in ...
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Medicine, Issue 56, firefly luciferase, Renilla Luciferase, breast cancer, metastasis, Smad. 3245 ... First, we will describe the steps necessary to construct dual bioluminescent reporter cell lines, as well as those needed to ... In vivo Dual Substrate Bioluminescent Imaging. Authors: Michael K. Wendt, Joseph Molter, Christopher A. Flask, William P. ... To do so, researchers take advantage for two light-producing luciferase enzymes isolated from the firefly (Photinus pyralis) ...
Frontiers | Enhanced BRET Technology for the Monitoring of Agonist-Induced and Agonist-Independent Interactions between GPCRs...
... a variant of Renilla luciferase, and a second protein of interest (β-arrestin) to an acceptor fluorophore. If in close ... a variant of Renilla luciferase, and a second protein of interest (β-arrestin) to an acceptor fluorophore. If in close ... Using novel luciferase constructs, we were able to monitor interactions not detectable using less sensitive BRET combinations ... Using novel luciferase constructs, we were able to monitor interactions not detectable using less sensitive BRET combinations ...
ReniformisGaussiaSubstrateProteinsGenesLuciferinProteinRLucPansyVivoPhotinusReportersEnzymesSubstratesReagentThermo ScientificLuminescenceAssay systemIntracellularBRETReactionsReagentsClick-beetleGlucCell lysatesOxidationOrganismsFirefly luciferase cDNANanoLucBacterialReactionCopepodActivity in mammalianDual luciferase reporterDetectionLuminometerBrighterMarine luciferasesDifferent luciferasesCypridinaFluorescent and bioluminescentReporter EnzymePromoterNovel bioluminescentCDNACellsFireflies36kDa
- The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis . (thermofisher.com)
- The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis . (fishersci.ca)
- Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter for normalizing transfection efficiency and for studying gene regulation and function. (moleculardevices.com)
- Renilla-luciferin 2-monooxygenase, Renilla luciferase, or RLuc, is a bioluminescent enzyme found in Renilla reniformis, belonging to a group of coelenterazine luciferases. (wikipedia.org)
- Of this group of enzymes, the luciferase from Renilla reniformis has been the most extensively studied, and due to its bioluminescence requiring only molecular oxygen, has a wide range of applications, with uses as a reporter gene probe in cell culture, in vivo imaging, and various other areas of biological research. (wikipedia.org)
- In Renilla reniformis, RLuc is found in membrane-bound intracellular structures within specialized light emitting cells, and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein (RrLBP). (wikipedia.org)
- 1962). In 1971 Morin and Hastings described very similar green fluorescent proteins isolated from Obelia (phylum Cnidaria, class Hydrozoa) and the sea pansy Renilla reniformis (phylum Cnidaria, class Anthozoa). (scholarpedia.org)
- To do so, researchers take advantage for two light-producing luciferase enzymes isolated from the firefly ( Photinus pyralis ) and sea pansy ( Renilla reniformis ), both of which react to mutually exclusive substrates that previously facilitated their wide-spread use in in vitro cell-based reporter gene assays 4 . (jove.com)
- To explore the functionality of these binding sites in the differential expression of EFG1 , we generated targeted deletions of each of the 10 binding sites, combinatorial deletions, and regional deletions using a Renilla reniformis luciferase reporter system. (g3journal.org)
- Unlike the first generation of luciferases, like Photinus pyralis luciferase (Fluc) and Renilla reniformis luciferase (Rluc), Gluc is naturally secreted by cells. (jcancer.org)
- Commonly used luciferase genes, such as the luciferases from the sea pansy ( Renilla reniformis ) and the North American firefly ( Photinus pyralis ) are currently used as transgene reporters. (aacrjournals.org)
- Prolume's three green fluorescent proteins (GFPs) from marine anthozoans (Renilla mulleri, Renilla reniformis and Ptilosarcus) are intrinsically brighter than any other GFPs on the market, including the brightest variants of the well-known Aequorea GFP. (prolume.com)
- Prolume also synthesizes bulk quantities of coelenterazine, the luciferin natively utilized by the photoprotein Aequorin and many luciferases, including those of Renilla reniformis and Gaussia. (prolume.com)
- Several bioluminescent coelenterates such as the jellyfish Aequorea victoria or the sea pansy Renilla reniformis emit green light from a green fluorescent protein (GFP). (bbk.ac.uk)
- photoprotein or a luciferase-oxyluciferin complex in A. victoria and R. reniformis , respectively. (bbk.ac.uk)
- The fact that the putative Hermodice carunculata luciferase shows highest homology to the luciferase of a phylogenetically distant cnidarian (Renilla reniformis) can probably be attributable to the lack of publicly available luciferase sequences from more closely related organisms. (nih.gov)
- The second system includes the luciferases from the marine organisms Renilla reniformis (a cnidarian) and Gaussia princeps (a copepod) and the substrate coelenterazine. (imperialinnovations.co.uk)
- The chemistry of the renilla luciferase, gaussia luciferase and coelentrazine (BioLume, Inc.) is described. (sages.org)
- Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, Renilla , Gaussia , and Cypridina luciferases, and calcium-activated photoproteins. (springer.com)
- We tested the reliability of a minimally invasive procedure, based on a secreted luciferase (Gaussia luciferase), to frequently monitor the changes of glioma size. (jcancer.org)
- Secreted alkaline phosphatase (SEAP) and Gaussia luciferase (Gluc) are the most used liquid fluid reporters. (jcancer.org)
- Gluc is one of the smallest luciferase (19.9 kDa), isolated from the copepod Gaussia princeps . (jcancer.org)
- Prolume is also currently developing a patented copepod luciferase (Gaussia princeps) that is exceptionally bright, small and biochemically robust. (prolume.com)
- Renilla/Gaussia-Juices: Can I mix the substrate into the buffer and store it then? (pjk-gmbh.de)
- Renilla/Gaussia-Juices: High background without any enzyme adding? (pjk-gmbh.de)
- Why is the background level with Renilla or Gaussia Juice above the usual? (pjk-gmbh.de)
- A growing proportion of these systems use coelenterazine or analogues of imidazo[1,2-a]pyrazine luciferins along with photoproteins or luciferases from sea creatures such as Aequorea, Renilla, Gaussia or Oplophorus. (pasteur.fr)
- Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit. (fishersci.co.uk)
- Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. (fishersci.co.uk)
- The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions. (fishersci.co.uk)
- Gaussia luciferase has been cloned, overexpressed in bacteria, and used as a sensitive analytical reporter for hybridization assays and monitoring of cellular expression in culture and in vivo (Tannous et al. (justjason.com)
- The kit also is compatible for flash assays with other Renilla luciferases and derivatives that use coelenterazine as a substrate. (thermofisher.com)
- In BLI applications, a luciferin (the substrate coelenterazine) when combined with a luciferases (protein or enzyme) in the presence of oxygen produces visible light at the 480 nm wavelength. (sages.org)
- As shown in the animated videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. (thermofisher.com)
- Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. (figshare.com)
- However, recent advancements in bioluminescent imaging now make it possible to simultaneously quantify specific spatiotemporal changes in gene expression as a function of tumor development and metastatic progression via the use of dual substrate luminescence reactions. (jove.com)
- The BRET 1 system uses the luciferase substrate coelenterazine h (benzyl-coelenterazine), with light emission peaking at ∼480 nm. (frontiersin.org)
- The mechanism of light emission is broadly conserved: an enzymatic oxidation reaction by a luciferase enzyme turns a luciferin substrate into a high energy intermediate, which decays to produce light ( Shimomura, 2006 ). (elifesciences.org)
- Firefly luciferase catalyzes the transformation of its substrate d -luciferin into oxyluciferin in an oxygen and ATP-dependent process, leading to the emission of photons at wavelengths from 500 to 620 nm at a sufficient intensity to penetrate small animal tissues. (aacrjournals.org)
- A single reporter gene can be investigated for a single substrate doubly labeled with different signatures such as a radioactive nuclide (suitable for radionuclide imaging) or a nonradioactive paramagnetic/bioluminescent/fluorescent molecule (suitable for magnetic resonance or optical imaging) and thus can be imaged by different imaging modalities. (aacrjournals.org)
- Spontaneous emission of luminescence by a particular PPI-induced complementation of the split Renilla luciferase together with its membrane-permeable substrate, coelenterazine, allows noninvasive imaging of the sites and time of its occurrence in living cells. (europeanmedical.info)
- Despite their differences and separate evolutionary origins, all bioluminescent systems that have been studied produce light by oxidation of a light-emitting substrate (generically referred to as a luciferin) catalyzed by an enzyme (a luciferase). (biomedcentral.com)
- When mixed, the substrate for the firefly system (luciferin) and the glowworm luciferase do not produce light [ 9 ], which implies that they use different luciferase enzymes and substrates. (biomedcentral.com)
- Often genetic modifications in the luciferase structure or chemical modifications of their substrate have added new functionalities making these assays even more robust. (els.net)
- Bioluminescence is light producing event caused by luciferase enzyme mediated substrate catalysis. (els.net)
- Since the substrate requirements of NanoLuc are different from those of firefly luciferase, dual bioluminescence imaging for the simultaneous characterization of two lines, or two separate biological processes, is possible, as demonstrated in this work. (biomedcentral.com)
- 18. Kit according to claim 17, further comprising a luciferase substrate. (patentsencyclopedia.com)
- Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence. (addgene.org)
- It is known that this bioluminescence of the firefly emit a luminescence by chemically reacting with the luciferin which is luminescent substrate through the carton of the luciferase which is luminescent enzyme. (patentgenius.com)
- h-CTZ is also the preferred substrate for Renilla Luciferase in HTS (high throughput screens) due to its prolonged glow kinetic. (nanolight.com)
- The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate. (nanolight.com)
- The enzyme catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. (abcam.com)
- The first system is represented by the firefly luciferase (FFluc) from Photinus pyralis which uses D-luciferin (a benzothiazole) as substrate, is dependent on ATP and results in the production of yellow-green light (557 nm). (imperialinnovations.co.uk)
- In the presence of luciferin or coelenterazine, respectively, the bioluminescent proteins expressed from these mRNAs release visible light. (trilinkbiotech.com)
- The BRET method utilizes heterologous co-expression of fusion proteins linking one protein of interest (GPCR) to a bioluminescent donor enzyme, a variant of Renilla luciferase, and a second protein of interest (β-arrestin) to an acceptor fluorophore. (frontiersin.org)
- Scientists have spent a lot of time analyzing the genes and proteins that Renilla uses to create light, including a key enzyme known as luciferase. (mbari.org)
- We have engineered lentiviral vectors bearing fusions between different combinations of fluorescent and bioluminescent marker proteins and used bioluminescence imaging and intravital-scanning microscopy in real time to study the fate of human neural stem cells (hNSCs) at a cellular resolution in glioma-bearing brains in vivo . (jneurosci.org)
- In the current study, we have engineered a variety of lentiviral vectors to express fusions between fluorescent and bioluminescent proteins and show that both fusion partners are fully functional. (jneurosci.org)
- Prolume has cloned and patented several genes from bioluminescent marine organisms that encode fluorescent and luminescent proteins. (prolume.com)
- Prolume also holds broad patents covering the use of bioluminescent proteins in consumer products, including foods and beverages, toys and novelties, and entertainment and education applications. (prolume.com)
- Three of these proteins are members of the ANL superfamily of adenylating enzymes, with similar amino acid sequences to that of the luciferase enzyme found in fireflies (31 to 37 % identical), and are candidate luciferases for the glowworm bioluminescent system. (biomedcentral.com)
- The signal intensity of NanoLuc® luciferase provides a sensitive reporter that can be used to investigate low-level protein expression and endogenous proteins. (promegaconnections.com)
- Pending patents on the novel use of bioluminescent proteins on silicon chips for inexpensive high throughput screening and diagnostic applications. (nanolight.com)
- In this study, we evaluated the rapamycin-mediated interaction of the human proteins FK506-binding protein (FKBP12) rapamycin-binding domain (FRB) and FKBP12 by constructing a fusion of these proteins with a split- Renilla luciferase or a split enhanced green fluorescent protein (split-EGFP) such that complementation of the reporter fragments occurs in the presence of rapamycin. (aacrjournals.org)
- This protein fragment-assisted complementation strategy has been used with a variety of reporter proteins, including dihydrofolate reductase, β-Lactamase, green fluorescent protein (GFP), firefly luciferase and Renilla luciferase, and has been found to be a useful technique for studying protein-protein interactions in both bacteria and mammalian cells ( 8 - 12 ). (aacrjournals.org)
- Evolutionary relationship among the structure of various luciferase proteins or their organic chemical substrates are hard to establish, with little or no commonality. (els.net)
- Luciferase proteins obtained from diverse organisms are used as bioluminescent reporters because they enable monitoring of intracellular events with high sensitivity, and without destroying the cells or tissues. (els.net)
- While the number of fluorescent proteins available for imaging has undergone a great expansion over the last two decades, enabling simultaneous visualization of multiple molecular and cellular events, expansion of available luciferases has lagged behind. (biomedcentral.com)
- The genes of many light-emitting proteins, photoproteins and luciferases, were cloned. (sfu-kras.ru)
- Highly sensitive analytical systems for diagnosing a number of biologically active compounds - hormones, oncogenes, infectious agents, etc. were developed on the basis of bioluminescent reactions of these proteins. (sfu-kras.ru)
- Several enzymes are commonly used as reporter proteins, among them are chloramphenicol acetyl transferase (CAT), -galactosidase, human growth hormone (hGH) and luciferase. (abcam.com)
- Although luminescent proteins, including aequorin and luciferases, have been used to image organisms, the light intensity of these proteins is insufficient to provide temporal resolution. (scienceexchange.com)
- For bioluminescent imaging, we offer reporter genes luciferase, firefly luciferase (FLuc) and Renilla luciferase mRNAs. (trilinkbiotech.com)
- Novel GFP genes isolated from Ptilosarcus gurneyi and Renilla Mulleri with distinctive properties including a novel chromophore sequence that differs in 3 of 6 residues from the Aequorea GFP. (nanolight.com)
- Proprietary functional screening methods to identify new bioluminescent genes. (nanolight.com)
- Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. (perkinelmer.com)
- To compare the ability of the FGF-1 IRES to drive long term expression against the EMCV and FGF-2 IRESs, we performed analyses of expression kinetics using bicistronic vectors that express the bioluminescent renilla and firefly luciferase reporter genes. (biomedcentral.com)
- Because both compounds dual reporters are commonly used, most notably the reduce Renilla luminescence with increasing dose, it is not bioluminescent firefly and Renilla luciferase genes. (medpdffinder.com)
- Human breast cancer cells (MDA-MB-231) and anaplastic thyroid cancer cells (CAL62) were transduced with lentiviral particles, to express the Renilla luciferase and mCherry (mCherry-Rluc) reporter genes. (medsci.org)
- Human bone marrow-derived MSCs were transduced with lentiviral particles, to express the firefly luciferase and enhanced green fluorescence protein (Fluc2-eGFP) reporter genes (MSC/Fluc). (medsci.org)
- Luciferase Genes as Reporter Reactions: How to Use Them in Molecular Biology? (springerprofessional.de)
- The aim of this chapter is to provide the reader with an overview of state-of-the-art bioluminescent tools based on luciferase genes, highlighting molecular biology strategies that have been applied so far, together with some selected examples. (springerprofessional.de)
- 2005). 7 Coelenterazine Dependent Luciferases 9 Fig. 3 Origins of Glow-light reporter genes used in uHTS. (justjason.com)
- Bacterial luciferases are encoded by the genes luxAB that form an operon (luxCDABE) together with three additional genes (luxCDE) whose products synthesise the longchain aldehyde. (imperialinnovations.co.uk)
- The name does not specifically refer to Renilla, but instead refers to Renilla-luciferin, a chemical also known as coelenterazine. (wikipedia.org)
- This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP. (thermofisher.com)
- Following ohe discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. (springer.com)
- For example, an enzyme known as luciferase in fireflies produces light by acting on a molecule called luciferin. (elifesciences.org)
- Unlike fluorescence imaging, bioluminescence imaging relies on the activity of enzymes (luciferases) that convert unique substrates (like luciferin and coelerentarazin) into light ( 7 ). (aacrjournals.org)
- Researchers have studied the biochemistry used by Arachnocampa to produce light [ 9 - 11 ], but many details remain elusive, including the identities of the luciferase and luciferin. (biomedcentral.com)
- Luciferase Reporter Gene Assay - Information The Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as. (assaygenie.com)
- In the last few decades, bioluminescent systems based on the expression of a luciferase and the addition of a luciferin to monitor the emission of light have become very important tools for biological investigations. (pasteur.fr)
- Three main luciferin-luciferase systems have been utilised for BLI. (imperialinnovations.co.uk)
- Bacterial luciferases, found in the terrestrial bacterium Photorhabdus luminescens and marine bacteria from the genera Vibrio and Photobacterium, constitute the third luciferin-luciferase system. (imperialinnovations.co.uk)
- Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression. (thermofisher.com)
- Firefly luciferase is a 62-kDa protein that is active as a monomer and does not require subsequent processing for its activity. (moleculardevices.com)
- According to the specific bioluminescent protein, different substrates and protocols must be applied in the experimental procedure for BL measurement. (springer.com)
- The presence of a fluorescent component in the bioluminescent organs of Aequorea victoria jellyfish (phylum Cnidaria, class Hydrozoa) ( Figure 2 ) was noted by Davenport and Nicol in 1955 (Davenport and Nicol 1955), but it was Osamu Shimomura of Princeton University who was the first to realize that this fluorophore was actually a protein . (scholarpedia.org)
- In a footnote to his paper describing the isolation of the bioluminescent protein aequorin from Aequorea , Shimomura wrote, "A protein giving solutions that look slightly greenish in sunlight though only yellowish under tungsten lights, and exhibiting a very bright, greenish fluorescence in the ultraviolet of a Mineralite [a handheld ultraviolet lamp], has also been isolated from squeezates" (Shimomura et al. (scholarpedia.org)
- GPCR) to a bioluminescent donor enzyme, a variant of Renilla luciferase (luminophore: Rluc, hRluc, Rluc2, or Rluc8), and a second protein of interest (e.g., β-arrestin) to an acceptor fluorophore, a variant of green fluorescent protein (Venus or GFP10). (frontiersin.org)
- Here we tag protein #1 wtih renilla luciferase (rLuc- a bioluminescent protein) and protein #2 with YFP, if and only if protein 1 and 2 interact, the bioluminescent energy generated by rLuc protein is absorbed by the YFP protein, resulting in fluorescence, which we detect in a fluorimeter machine. (openwetware.org)
- Efficient bioluminescence resonance energy transfer (BRET) from a bioluminescent protein to a fluorescent protein with high fluorescent quantum yield has been utilized to enhance luminescence intensity, allowing single-cell imaging in near real time without external light illumination. (spie.org)
- We have applied this strategy to develop an autoluminescent Ca 2+ indicator, BRAC, which is composed of Ca 2+ -binding protein, calmodulin, and its target peptide, M13, sandwiched between a yellow fluorescent protein variant, Venus, and an enhanced Renilla luciferase, RLuc8. (spie.org)
- In this study, a novel reporter vector was constructed encoding a fusion protein comprised of a mutant herpes simplex virus type 1 thymidine kinase ( HSV1-sr39tk ) ( tk ), a positron emission tomography (PET) reporter gene, and renilla luciferase ( rl ), a bioluminescence optical reporter gene joined by a 20 amino acid long spacer sequence. (aacrjournals.org)
- Graph showing the luciferase activity of lysates prepared from cells expressing the Rluc protein at various salt concentrations. (sciencematters.io)
- Chemical conjugation of the protein label with either the analyte or the binder (e.g., antibody or protein A) is traditionally used in order to obtain tracers suitable for developing bioluminescent or fluorescent immunoassays. (europeanmedical.info)
- NanoLuc® luciferase has been discussed many times on this blog and our web site because the enzyme is integral to studying genetic responses and protein dynamics. (promegaconnections.com)
- While NanoLuc® luciferase was first introduced as a reporter enzyme to assess promoter activity, its capabilities have expanded far beyond a genetic reporter, creating tools used to study endogeneous protein interactions, target engagement, protein degradation and more. (promegaconnections.com)
- We took it a step further and partnered NanoLuc® luciferase with the HaloTag® protein and its fluorescent ligands to create the NanoBRET™ assay. (promegaconnections.com)
- While NanoLuc® luciferase may be a small reporter enzyme, having a small peptide only 11 amino acids would be useful for measuring endogenous protein abundance. (promegaconnections.com)
- Recent studies from our lab on protein fragment complementation using split firefly luciferase or split- Renilla luciferase (split-RLuc) have shown their use for studying both protein-protein interactions and small molecule-mediated protein-protein interaction noninvasively in small animals ( 13 , 14 ). (aacrjournals.org)
- The study of intracellular and extracellular dimerization kinetics of the protein-protein interaction of FK506-binding protein (FKBP12) rapamycin-binding domain (FRB) and FKBP12 mediated by the small molecule rapamycin and the associated Renilla luciferase complementation signals have been found to be relatively weak ( 11 ). (aacrjournals.org)
- In biotechnology, mutations can be performed on luciferase gene sequence obtained from nature to make it suitable for use in higher order organisms and/or attribute better characters such as brightness, change spectral properties or protein stability. (els.net)
- Further, using the Dual-Luciferase Reporter Assay, they identified VPS37A (Vascular Protein Sorting 37 homolog A) as the downstream effector or miRNA-19a regulation. (promegaconnections.com)
- To date, no bioluminescent protein sequence has been reported from this phylum, but we do report homologous sequences of a luciferase protein (Figure 5). (nih.gov)
- b) analyzing energy resonance transfer under conditions wherein at least the BRET sensor or its bioluminescent donor protein component is immobilized or absorbed to a solid carrier. (patentsencyclopedia.com)
- These are the method of simultaneous detection of two targets in one sample on the basis of color variants of Ca2+-regulated photoprotein obelin and the method for rapid and sensitive detection of tick-borne encephalitis virus based on the unique biospecific fusion protein of Renilla luciferase. (sfu-kras.ru)
- Fusion protein corresponding to Firefly Firefly Luciferase. (abcam.com)
- Hybridoma produced by the fusion of splenocytes from mice immunized with luciferase protein isolated from Photinus pyralis. (abcam.com)
- The use of an antibody to detect luciferase can provide an alternative detection assay which directly detects luciferase protein levels, and thus has the advantage that it does not require luciferase activity and is not dependent on rapid kinetics. (abcam.com)
- Reacts with Luciferase (Firefly) Protein. (abcam.com)
- To improve the brightness, we recently developed a luminescent protein, Nano-lantern, that is a chimera of enhanced Renilla luciferase (3) and Venus (4), a fluorescent protein with high BRET efficiency that is 10 times brighter than RLuc5. (scienceexchange.com)
- Using Renilla luciferase (Rluc)-DsRed2 or GFP-Rluc-expressing malignant human glioma model, transduced hNSCs were shown to migrate extensively toward gliomas, with hNSCs populating gliomas at 10 d after transplantation. (jneurosci.org)
- Noninvasive serial imaging of luciferase signal time kinetics in representative mice with either tumour xenograft (upper panel) or metastatic tumours in the lungs (lower panel) by implanting with HT1080 human fibrosarcoma cells over‐expressing RLuc reporter. (els.net)
- The poster child for glowing octocorals is the sea pansy, a shallow-water sea pen in the genus Renilla . (mbari.org)
- Although the luciferase catalyzed oxidation of coelenterazine releases a photon of blue light (480 nm), this is not observed in vivo. (wikipedia.org)
- Bioluminescent reporters are useful for time-course studies in cells and in vivo . (trilinkbiotech.com)
- Our understanding of how and when breast cancer cells transit from established primary tumors to metastatic sites has increased at an exceptional rate since the advent of in vivo bioluminescent imaging technologies 1-3 . (jove.com)
- In vivo bioluminescence imaging depends on light emitted by luciferases in the body overcoming the effect of tissue attenuation. (nih.gov)
- In vivo imaging system is a cooled charge-coupled device that measures bioluminescent light. (aacrjournals.org)
- Noninvasive small animal imaging using luciferase reporter is gaining ground to provide simultaneous qualitative and quantitative measurements of disease interrogation in vivo . (els.net)
- To observe bacteria in action, researchers have developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. (addgene.org)
- In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint what tissue or organ this signal is coming from. (addgene.org)
- We have investigated for the first time the dynamics and the kinetics of tachyzoite-to-bradyzoite interconversion and cyst formation in vivo by using stage-specific bioluminescent parasites in a mouse model. (asm.org)
- Lentiviral particles for tranduction of mammalian cell lines with a green emitting luciferase transgene, for use in in vivo and other applications. (perkinelmer.com)
- Bioluminescence, the production of light by luciferase-catalysed reactions, is a versatile reporter technology with multiple applications both in vitro and in vivo. (imperialinnovations.co.uk)
- In vivo bioluminescence imaging (BLI) represents one of its most outstanding uses by allowing the non-invasive localisation of luciferase-expressing cells within an animal. (imperialinnovations.co.uk)
- Firefly Luciferase Assay Kit Luciferase Assay Kit (from the firefly Photinus pyralis) is an accurate, sensitive and easy method for studying gene reporter regulation and function in. (assaygenie.com)
- The most widely used bioluminescent probe in malaria research is the Photinus pyralis firefly luciferase, followed by the more recently introduced Click-beetle and Renilla luciferases. (biomedcentral.com)
- With the exception of bioluminescent reporter NF-κB reporter mice for biophotonic imaging, the majority of strains use fluorescent reporters for two-photon imaging modalities. (jimmunol.org)
- However, current bioluminescent reporters do present some significant challenges, which has limited their broad application for macro scale visualization of gene expression. (elifesciences.org)
- Plasmodium falciparum and several rodent Plasmodium strains have already been engineered to express bioluminescent reporters. (biomedcentral.com)
- The attractiveness of secreted luciferases as reporters is a strong stimulus for the investigation and exploitation of new bioluminescent systems. (justjason.com)
- Optimisation of bioluminescent reporters for use with mycobacteria. (imperialinnovations.co.uk)
- Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes. (figshare.com)
- Whereas luciferins can be shared by phylogenetically distant organisms which feed on organisms producing them, luciferases have been thought to be lineage-specific enzymes. (figshare.com)
- Our findings strongly indicate that those enzymes, belonging to the haloalkane dehalogenase family, might then have been convergently co-opted into luciferases in cnidarians and echinoderms. (figshare.com)
- We have studied four codon optimized luciferases with different emission spectra, including enzymes from firefly (FLuc), click beetle (CBGr68, CBRed) and Renilla reniformins (hRLuc). (nih.gov)
- Luciferase has become one of the widely used reporter enzymes. (abcam.com)
- These luciferases are heterodimeric enzymes that use FMNH2 and a long-chain aldehyde as substrates. (imperialinnovations.co.uk)
- The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. (thermofisher.com)
- The Pierce Renilla Flash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. (thermofisher.com)
- A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. (patentsencyclopedia.com)
- Our luciferase assay reagent options have a half-life of several hours, allowing for streamlined detection procedures. (perkinelmer.com)
- But to guarantee a proper cell lysis you should particular mix the sample with the reagent through several pipett mixing and wait at least 5 minutes before measurement.Important: It is not useful to combine Beetle Lysis-Juice with Renilla-Juice because of the autoluminescence from coelenterazine in presence of detergents. (pjk-gmbh.de)
- The firefly luciferase luminescence is measured first by addition of the FFL Reagent. (assaygenie.com)
- The RL Reagent simultaneously quenches the firefly luciferase luminescence and initiates the Renilla luciferase reaction. (assaygenie.com)
- Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells. (assaygenie.com)
- The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate. (thermofisher.com)
- When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. (thermofisher.com)
- Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers. (thermofisher.com)
- The Thermo Scientific™ pMCS-Renilla Luc Vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter. (fishersci.ca)
- The Thermo Scientific Pierce Cypridina -Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates. (thermofisher.com)
- Automated addition of firefly working solution by injection initiates the firefly luciferase luminescence reaction, and subsequent addition of Renilla working solution via a second injection quenches the firefly luminescence and adds Aquaphile™ coelenterazine to initiate the Renilla reaction. (moleculardevices.com)
- It contains Renilla LUCIFERASE which oxidizes coelenterazine resulting in LUMINESCENCE. (bvsalud.org)
- Upon 100-nM insulin stimulation, luminescence emitted by complement Renilla luciferase. (europeanmedical.info)
- Glow luminescence reporter gene assay combining benefits of both bright signal and long-lasting signal for firefly luciferase. (perkinelmer.com)
- 3. A method for detecting an luminescence comprising: reacting a compound according to claim 1 with luminescent beetle luciferase, and detecting an luminescence from the compound. (patentgenius.com)
- 2003) Chroma-Glo Luciferase Assay System Technical Manual No. TM062, 7-9. (springer.com)
- sensilite is an Enhanced Flash Firefly luciferase detection assay system designed to detect and quantitate Firefly luciferase from cultured cells with optimum sensitivity. (perkinelmer.com)
- twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. (perkinelmer.com)
- Interference in the reporter response can also arise when Promega has developed a new, homogeneous reporter underlying genetic events are masked by physiological assay, the Dual-Glo™ Luciferase Assay System, for factors such as cell viability. (medpdffinder.com)
- This is demonstrated in Panel Assay System(a,b,c) (3) for dual reporter measurements in A, where only Renilla luciferase activity was assayed. (medpdffinder.com)
- Like Promega's Dual- contrast, by using the firefly luciferase as an internal Luciferase® Reporter Assay System(a,b,c), the new Dual- reference, the distinction between genetic response and Glo™ Assay allows sequential measurement of both firefly cell death is clear. (medpdffinder.com)
- The The Dual-Glo™ Assay System was developed to support significance of this interference on reporter responses can these requirements by providing a simple, homogeneous be realized only through a properly configured reference, means of quantifying both the firefly and Renilla luciferases typically from a secondary reporter. (medpdffinder.com)
- Galluzzi, L., and Karp, M. (2007) Intracellular redox equilibrium and growth phase affect the performance of luciferase-based biosensors. (springer.com)
- Using novel luciferase constructs, we were able to monitor interactions not detectable using less sensitive BRET combinations in the same configuration. (frontiersin.org)
- With such a bright enzyme, NanoLuc® luciferase would be an ideal luminescent donor for bioluminescence resonance energy transfer (BRET) assays that measure molecular proximity. (promegaconnections.com)
- The activities of synthesized firefly and Renilla luciferases were co-determined in a single sample based on the requirements of their characteristic bioluminescent reactions. (uwindsor.ca)
- This bioluminescent dual reporter gene assay is extremely sensitive and is especially suitable for quantifying dual luciferase expression in recombinant cells or in cell free transcription/translation reactions. (assaygenie.com)
- This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. (thermofisher.com)
- Serial injection of two optimized detection reagents allows the luciferases to be assayed in the same microplate well. (moleculardevices.com)
- These proprietary technologies, which NanoLight has already developed or protected by patent filings & trade secrets, permit the company to grow into the dominant supplier of proprietary bioluminescent reagents and reporter systems to the life science markets. (nanolight.com)
- Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. (abcam.com)
- Stolz U, Velez S, Wood KV et al (2003) Darwinian natural selection for orange bioluminescent color in a Jamaican click beetle. (springerprofessional.de)
- The signal produced by the G. princeps luciferase (Gluc) has been reported to be stronger than that of FFluc, even though the light emitted is in the blue range (480 nm) and is therefore more susceptible to tissue absorption and scattering. (imperialinnovations.co.uk)
- The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. (perkinelmer.com)
- World's largest collection of expression libraries from marine bioluminescent organisms. (nanolight.com)
- The studies on bioluminescent systems of various luminous organisms are being carried out in the laboratory of photobiology of the Institute of Biophysics SB RAS for more than 30 years. (sfu-kras.ru)
Firefly luciferase cDNA2
- A third aspect of this dissertation combines the firefly luciferase cDNA and a cDNA encoding Renilla luciferase as labels for the development of a microtiter well-based expression hybridization assay that allows simultaneous determination of two target DNA sequences. (uwindsor.ca)
- De Wet JR, Wood KV, Helinski DR et al (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. (springerprofessional.de)
- So where did the NanoLuc® luciferase come from and how does a one enzyme power several technologies? (promegaconnections.com)
- The story of NanoLuc® luciferase starts with a deep sea shrimp and its bioluminescent enzyme that evolved with some tinkering from Promega scientists. (promegaconnections.com)
- Furthermore, the small size of the engineered NanoLuc® luciferase means the enzyme can be used to create reporter viruses without disrupting packaging or measure endogenous expression using CRISPR-Cas9 gene editing. (promegaconnections.com)
- Read how NanoLuc® luciferase was developed in this article . (promegaconnections.com)
- Want to learn more about NanoLuc® luciferase and its capabilities? (promegaconnections.com)
- Check out our NanoLuc® Luciferase technology page for additional resources and applications. (promegaconnections.com)
- With the purpose of overcoming these challenges, a Plasmodium berghei line expressing the novel ultra-bright luciferase enzyme NanoLuc, called PbNLuc has been generated, and is presented in this work. (biomedcentral.com)
- NanoLuc shows at least 150 times brighter signal than firefly luciferase in vitro, allowing single parasite detection in mosquito, liver, and sexual and asexual blood stages. (biomedcentral.com)
- c) Reduced FMN reacts with oxygen on bacterial luciferase to produce a metastable peroxyflavin on the luciferase. (els.net)
- 1995) Structure of bacterial luciferase. (els.net)
- Moreover, using luciferase as a reporter of gene expression, it is possible to establish when and where a gene function is needed, shedding light on bacterial pathogenesis. (imperialinnovations.co.uk)
- Photons can be generated by either fluorescent sources that emit light at one wavelength in response to excitation by light of a different wavelength ( 5 ) or by bioluminescent sources that produce light as a result of a chemical reaction ( 6 ). (aacrjournals.org)
- Markova SV, Golz S, Frank LA et al (2004) Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. (springerprofessional.de)
Activity in mammalian1
- It is a very sensitive reporter due to the lack of any endogenous luciferase activity in mammalian cells or tissue. (moleculardevices.com)
Dual luciferase reporter6
- The Dual Luciferase Reporter Assay allows for the sequential measurement of the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. (assaygenie.com)
- Product Information The Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the. (assaygenie.com)
- Below are our top picks of exciting research discoveries involving the Dual-Luciferase Reporter Assay format using firefly and Renilla luciferases. (promegaconnections.com)
- Using the Dual-Luciferase Reporter Assay, Reinhardt et al . (promegaconnections.com)
- Mutations affecting these secondary structures led to decreased infectivity and viral replication as tested by methods including the Dual-Luciferase Reporter Assay. (promegaconnections.com)
- We then used a pull-down assay and dual-luciferase reporter assay to identify the downstream miR-149-5p of circNRIP1. (springer.com)
- The Pierce Cypridina -Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. (thermofisher.com)
- Typically, filters are not used for detection of luciferase activity. (pjk-gmbh.de)
- High sensitivity and wide detection range: detection of as little of 2 fg luciferase. (assaygenie.com)
- Fast and convenient: three step assay allows detection of dual luciferase levels within 20 minutes. (assaygenie.com)
- Beetle-Juices: Will I need an automatic injection system to perform an assay with Firefly Luciferase with a luminometer? (pjk-gmbh.de)
- Cypridina luciferase produces brighter bioluminescent signal than firefly and native Renilla luciferases. (thermofisher.com)
- The result: A small 19kDa reporter enzyme with a luminescent signal 100X brighter than that of firefly or Renilla luciferase. (promegaconnections.com)
- the latter appears to be brighter than existing Renilla or Firefly systems in mammalian cells. (nanolight.com)
- Novel marine luciferases cloned from Renilla Mulleri, Pleuromama sp. (nanolight.com)
- In this study, we describe a novel cytotoxicity assay, termed the Matador assay, which takes advantage of the extreme brightness, stability and glow-like characteristics of recently discovered novel marine luciferases and their engineered derivatives. (paperity.org)
- But we offer a very reliable "dual well" system that means that the sample with two different luciferases can be split in 2 tubes/wells. (pjk-gmbh.de)
Fluorescent and bioluminescent1
- His approach for this has been to develop genetically encoded fluorescent and bioluminescent indicators to pinpoint each cellular process in single living cells. (or.jp)
- A novel secreted bioluminescent reporter enzyme. (springerprofessional.de)
- Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK). (fishersci.ca)
- The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. (fishersci.ca)
- Afterward, renilla luciferase expressing 4T1 cells were transfected with firefly luciferase the expression of which was driven from the human E cad promoter, followed by assortment with puromycin. (thrombin-inhibitor.com)
- luciferase under control of the Tet-Off promoter and fireflyluciferase under control of the CMV promoter (Figure 1). (medpdffinder.com)
- evaluation of cellular physiology (1,2), a single reporter Doxycycline is expected to specifically downregulate the may not convey sufficient information for reliable Renilla expression coupled to the Tet-Off promoter, while interpretation of the experimental data. (medpdffinder.com)
- RediFect™ Green Renilla-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles carrying green emitting Renilla luciferase transgene under control of the stable UbC promoter. (perkinelmer.com)
- Bioluminescence imaging (BLI) of metastatic 4T1 cells expressing cytomegalovirus (CMV)-driven Renilla luciferase and firefly luciferase driven by the epithelial cadherin (E-cad) promoter. (nih.gov)
- Understanding the molecular basis of light generation in glowworms will not only expand our understanding of how bioluminescence works, but may also lead to novel bioluminescent applications. (biomedcentral.com)
- Another aspect of this dissertation involves the development of two novel microtiter well-based DNA hybridization assays in which an expressible cDNA fragment encoding firefly luciferase serves as a reporter molecule. (uwindsor.ca)
- The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells. (moleculardevices.com)
- Dual bioluminescent 4T1 cells have been gener ated by transfection with pcDNA3. (thrombin-inhibitor.com)
- Investigators developed bioluminescent extracellular vesicles using Renilla luciferase -expressing mesenchymal stem cells. (mesenchymalcellnews.com)
- Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells. (perkinelmer.com)
- Reporter gene assay with longest-lasting signal for quantitation of firefly luciferase in mammalian cells. (perkinelmer.com)
- Luciferase activity of the transduced cells was measured by bioluminescence imaging (BLI). (medsci.org)
- The assay involves expression of a luciferase of interest in target cells in a manner so that it is preferentially retained within the healthy cells but is either released from dead and dying cells or whose activity can be preferentially measured in dead and dying cells. (paperity.org)
- The bioluminescent system of the glowworm is evolutionarily distinct from other well-characterised systems, especially that of the fireflies, and the molecules involved have not yet been identified. (biomedcentral.com)
- Confusingly, some firefly larvae and adults are also referred to as glowworms, but the well-studied bioluminescent beetles (including fireflies, click beetles and railroad worms) are members of a different order - Coeloptera (superfamily Elateroidea) [ 4 ]. (biomedcentral.com)
- The gene encodes a bioluminescent Green Renilla luciferase (36kDa). (fishersci.ca)