Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
Tetrahydroisoquinolinol alkaloids in both dextro and levo forms, originally found in SALSOLA plants.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Neoplasms composed of tissues of the OVARY or the TESTIS, not neoplasms located in the ovaries or testes. Gonadal tissues include GERM CELLS, cells from the sex cord, and gonadal stromal cells.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The exposure to potentially harmful chemical, physical, or biological agents in the environment or to environmental factors that may include ionizing radiation, pathogenic organisms, or toxic chemicals.
The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.
Specific proteins found in or on cells of progesterone target tissues that specifically combine with progesterone. The cytosol progesterone-receptor complex then associates with the nucleic acids to initiate protein synthesis. There are two kinds of progesterone receptors, A and B. Both are induced by estrogen and have short half-lives.
Tumors or cancer of the human BREAST.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. It has extensive homology to and heterodimerizes with the EGF RECEPTOR, the ERBB-3 RECEPTOR, and the ERBB-4 RECEPTOR. Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member.
Elements of limited time intervals, contributing to particular results or situations.
An enzyme, sometimes called GGT, with a key role in the synthesis and degradation of GLUTATHIONE; (GSH, a tripeptide that protects cells from many toxins). It catalyzes the transfer of the gamma-glutamyl moiety to an acceptor amino acid.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
Carcinogenic substances that are found in the environment.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Substances or energies, for example heat or light, which when introduced into the air, water, or land threaten life or health of individuals or ECOSYSTEMS.
A primary, chronic disease with genetic, psychosocial, and environmental factors influencing its development and manifestations. The disease is often progressive and fatal. It is characterized by impaired control over drinking, preoccupation with the drug alcohol, use of alcohol despite adverse consequences, and distortions in thinking, most notably denial. Each of these symptoms may be continuous or periodic. (Morse & Flavin for the Joint Commission of the National Council on Alcoholism and Drug Dependence and the American Society of Addiction Medicine to Study the Definition and Criteria for the Diagnosis of Alcoholism: in JAMA 1992;268:1012-4)
Cytoplasmic proteins that bind estrogens and migrate to the nucleus where they regulate DNA transcription. Evaluation of the state of estrogen receptors in breast cancer patients has become clinically important.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
The conic organs which usually give outlet to milk from the mammary glands.
The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.
A graphic means for assessing the ability of a screening test to discriminate between healthy and diseased persons; may also be used in other studies, e.g., distinguishing stimuli responses as to a faint stimuli or nonstimuli.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Levels within a diagnostic group which are established by various measurement criteria applied to the seriousness of a patient's disorder.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay.
A class of statistical procedures for estimating the survival function (function of time, starting with a population 100% well at a given time and providing the percentage of the population still well at later times). The survival analysis is then used for making inferences about the effects of treatments, prognostic factors, exposures, and other covariates on the function.
A peptide hormone that lowers calcium concentration in the blood. In humans, it is released by thyroid cells and acts to decrease the formation and absorptive activity of osteoclasts. Its role in regulating plasma calcium is much greater in children and in certain diseases than in normal adults.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
A soft, grayish metal with poisonous salts; atomic number 82, atomic weight 207.19, symbol Pb. (Dorland, 28th)
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.
A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Microtubule-associated proteins that are mainly expressed in neurons. Tau proteins constitute several isoforms and play an important role in the assembly of tubulin monomers into microtubules and in maintaining the cytoskeleton and axonal transport. Aggregation of specific sets of tau proteins in filamentous inclusions is the common feature of intraneuronal and glial fibrillar lesions (NEUROFIBRILLARY TANGLES; NEUROPIL THREADS) in numerous neurodegenerative disorders (ALZHEIMER DISEASE; TAUOPATHIES).
A set of techniques used when variation in several variables has to be studied simultaneously. In statistics, multivariate analysis is interpreted as any analytic method that allows simultaneous study of two or more dependent variables.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
Period after successful treatment in which there is no appearance of the symptoms or effects of the disease.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
Preliminary cancer therapy (chemotherapy, radiation therapy, hormone/endocrine therapy, immunotherapy, hyperthermia, etc.) that precedes a necessary second modality of treatment.
Tumors or cancer of the LUNG.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
A severe emotional disorder of psychotic depth characteristically marked by a retreat from reality with delusion formation, HALLUCINATIONS, emotional disharmony, and regressive behavior.
Studies in which subsets of a defined population are identified. These groups may or may not be exposed to factors hypothesized to influence the probability of the occurrence of a particular disease or other outcome. Cohorts are defined populations which, as a whole, are followed in an attempt to determine distinguishing subgroup characteristics.
Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.
A country in western Europe bordered by the Atlantic Ocean, the English Channel, the Mediterranean Sea, and the countries of Belgium, Germany, Italy, Spain, Switzerland, the principalities of Andorra and Monaco, and by the duchy of Luxembourg. Its capital is Paris.
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
A malignant epithelial tumor with a glandular organization.
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system.
Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable.
Behaviors associated with the ingesting of alcoholic beverages, including social drinking.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Tumors or cancer of the STOMACH.
A syndrome characterized by progressive life-threatening RESPIRATORY INSUFFICIENCY in the absence of known LUNG DISEASES, usually following a systemic insult such as surgery or major TRAUMA.
A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5)
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Studies in which the presence or absence of disease or other health-related variables are determined in each member of the study population or in a representative sample at one particular time. This contrasts with LONGITUDINAL STUDIES which are followed over a period of time.
The relationship between the dose of an administered drug and the response of the organism to the drug.
DNA present in neoplastic tissue.
Stress wherein emotional factors predominate.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Any method used for determining the location of and relative distances between genes on a chromosome.
The local recurrence of a neoplasm following treatment. It arises from microscopic cells of the original neoplasm that have escaped therapeutic intervention and later become clinically visible at the original site.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
A latent susceptibility to disease at the genetic level, which may be activated under certain conditions.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Warfare involving the use of living organisms or their products as disease etiologic agents against people, animals, or plants.
Studies in which variables relating to an individual or group of individuals are assessed over a period of time.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
The qualitative or quantitative estimation of the likelihood of adverse effects that may result from exposure to specified health hazards or from the absence of beneficial influences. (Last, Dictionary of Epidemiology, 1988)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
A plan for collecting and utilizing data so that desired information can be obtained with sufficient precision or so that an hypothesis can be tested properly.
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
Statistical models used in survival analysis that assert that the effect of the study factors on the hazard rate in the study population is multiplicative and does not change over time.
Age as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or the effect of a circumstance. It is used with human or animal concepts but should be differentiated from AGING, a physiological process, and TIME FACTORS which refers only to the passage of time.
Biological activities and function of the whole organism in human, animal, microorgansims, and plants, and of the biosphere.
Statistical models which describe the relationship between a qualitative dependent variable (that is, one which can take only certain discrete values, such as the presence or absence of a disease) and an independent variable. A common application is in epidemiology for estimating an individual's risk (probability of a disease) as a function of a given risk factor.
Established cell cultures that have the potential to propagate indefinitely.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Inhaling and exhaling the smoke of burning TOBACCO.
Tests designed to assess neurological function associated with certain behaviors. They are used in diagnosing brain dysfunction or damage and central nervous system disorders or injury.
The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.
Drug therapy given to augment or stimulate some other form of treatment such as surgery or radiation therapy. Adjuvant chemotherapy is commonly used in the therapy of cancer and can be administered before or after the primary treatment.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
The return of a sign, symptom, or disease after a remission.
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
Sequential operating programs and data which instruct the functioning of a digital computer.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Computer-based representation of physical systems and phenomena such as chemical processes.
Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Use of naturally-occuring or genetically-engineered organisms to reduce or eliminate populations of pests.
A cell line derived from cultured tumor cells.
Genotypic differences observed among individuals in a population.
The relationships of groups of organisms as reflected by their genetic makeup.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Materials used as reference points for imaging studies.
The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.
Databases devoted to knowledge about specific genes and gene products.
Comprehensive, methodical analysis of complex biological systems by monitoring responses to perturbations of biological processes. Large scale, computerized collection and analysis of the data are used to develop and test models of biological systems.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Antibodies produced by a single clone of cells.
Interacting DNA-encoded regulatory subsystems in the GENOME that coordinate input from activator and repressor TRANSCRIPTION FACTORS during development, cell differentiation, or in response to environmental cues. The networks function to ultimately specify expression of particular sets of GENES for specific conditions, times, or locations.
Proteins prepared by recombinant DNA technology.
A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The rate dynamics in chemical or physical systems.
The physiological mechanisms that govern the rhythmic occurrence of certain biochemical, physiological, and behavioral phenomena.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Genetic loci associated with a QUANTITATIVE TRAIT.
One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.
Deoxyribonucleic acid that makes up the genetic material of plants.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The protein complement of an organism coded for by its genome.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The ratio of radiation dosages required to produce identical change based on a formula comparing other types of radiation with that of gamma or roentgen rays.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Methods for determining interaction between PROTEINS.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived.
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (1/39254)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Serum triglyceride: a possible risk factor for ruptured abdominal aortic aneurysm. (2/39254)

BACKGROUND: We aimed to determine the relationship between ruptured abdominal aortic aneurysm (AAA) and serum concentrations of lipids and apolipoproteins. METHODS: A cohort of 21 520 men, aged 35-64 years, was recruited from men attending the British United Provident Association (BUPA) clinic in London for a routine medical examination in 1975-1982. Smoking habits, weight, height and blood pressure were recorded at entry. Lipids and apolipoproteins were measured in stored serum samples from the 30 men who subsequently died of ruptured AAA and 150 matched controls. RESULTS: Triglyceride was strongly related to risk of ruptured AAA. In univariate analyses the risk in men on the 90th centile of the distribution relative to the risk in men on the 10th (RO10-90) was 12 (95% confidence interval [CI] : 3.8-37) for triglyceride, 5.5 (95% CI: 1.8-17) for apolipoprotein B (apoB) (the protein component of low density lipoprotein [LDL]), 0.15 (95% CI : 0.04-0.56) for apo A1 (the protein component of high density lipoprotein [HDL]), 3.7 (95% CI: 1.4-9.4) for body mass index and 3.0 (95% CI: 1.1-8.5) for systolic blood pressure. Lipoprotein (a) (Lp(a)) was not a significant risk factor (RO10-90 = 1.6, 95% CI: 0.6-3.0). In multivariate analysis triglyceride retained its strong association. CONCLUSION: Triglyceride appears to be a strong risk factor for ruptured AAA, although further studies are required to clarify this. If this and other associations are cause and effect, then changing the distribution of risk factors in the population (by many people stopping smoking and adopting a lower saturated fat diet and by lowering blood pressure) could achieve an important reduction in mortality from ruptured AAA.  (+info)

Constitutional, biochemical and lifestyle correlates of fibrinogen and factor VII activity in Polish urban and rural populations. (3/39254)

BACKGROUND: Fibrinogen and factor VII activity are known to be related to atherosclerosis and coronary heart disease, but population differences in clotting factors and modifiable characteristics that influence their levels have not been widely explored. METHODS: This paper examines correlates of plasma fibrinogen concentration and factor VII activity in 2443 men and women aged 35-64 in random samples selected from the residents in two districts in urban Warsaw (618 men and 651 women) and from rural Tarnobrzeg Province (556 men and 618 women) screened in 1987-1988, and assesses which characteristics might explain urban-rural differences. Fibrinogen and factor VII activity were determined using coagulation methods. RESULTS: Fibrinogen was 12.9 mg/dl higher in men and 14.1 mg/dl higher in women in Tarnobrzeg compared to Warsaw. Factor VII activity was higher in Warsaw (9.2% in men and 15.3% in women). After adjustment for selected characteristics, fibrinogen was higher in smokers compared to non-smokers by 28 mg/dl in men and 22 mg/dl in women. In women, a 15 mg/dl increase in HDL-cholesterol was associated with a 10 mg/dl decrease in fibrinogen (P < 0.01). After adjustment for other variables, a higher factor VII activity in Warsaw remained significant (a difference of 9.4% in men and 14.8% in women). Lower fibrinogen in Warsaw remained significant only in women (15.4 mg/dl difference). CONCLUSIONS: The study confirmed that sex, age, BMI, smoking and blood lipids are related to clotting factors. However, with the exception of gender differences and smoking, associations between clotting factors and other variables were small and of questionable practical importance.  (+info)

Hepatitis B--are surgeons putting patients at risk? (4/39254)

The 1993 Department of Health guidelines permit a surgeon who is hepatitis B surface antigen (HBsAg) positive but e-antigen (HBeAg) negative to perform exposure prone procedures, unless demonstrated to have infected patients. However, there is increasing evidence of transmission of hepatitis B to patients from health care workers in this supposedly low infectivity category. The Occupational Physician must decide whether existing guidelines represent an adequate risk assessment and indeed whether this is an acceptable risk for patients. If an NHS Trust continues to follow these guidelines it may be in breach of its duty of care to patients. Yet refusing to allow such carriers to operate without testing for additional serological markers may be unlawful discrimination. Further research is clearly needed as well as an urgent review of the guidelines.  (+info)

Measurement of serum TSH in the investigation of patients presenting with thyroid enlargement. (5/39254)

In otherwise euthyroid patients presenting with thyroid enlargement, reduction in serum thyrotrophin (TSH) concentrations measured in a sensitive assay may be a marker of thyroid autonomy and may therefore indicate a benign underlying pathology. We investigated prospectively a cohort of 467 subjects presenting consecutively to our thyroid clinic with nodular or diffuse enlargement of the thyroid. Subjects were divided into those with normal (0.4-5.5 mU/l), low but detectable (0.1-0.39 mU/l) or undetectable (< 0.1 mU/l) serum TSH concentrations. The final pathological diagnosis was defined by fine-needle aspiration cytology and clinical follow-up of at least 2 years or by fine-needle aspiration cytology and histology following surgical treatment. Serum TSH concentrations below normal were found in 75 patients (16.1%), those with low serum TSH results having higher mean free T4 concentrations, were older and were more likely to be female. In those with undetectable serum TSH, no patient had a diagnosis of thyroid neoplasia and in those with low but detectable TSH, thyroid neoplasms were diagnosed in two patients (3.4%). In those with normal serum TSH, 12.0% had a final diagnosis of thyroid neoplasm (p = 0.013). Overall, thyroid malignancy was found in one patient (1.3%) of those with a serum TSH measurement below the normal range and 6.9% of those with normal serum TSH (p < 0.06). Reduction in serum TSH at presentation may identify a group which requires less intensive investigation and follow-up than those without biochemical evidence of thyroid autonomy.  (+info)

Endocrine biomarkers of early fetal loss in cynomolgus macaques (Macaca fascicularis) following exposure to dioxin. (6/39254)

This study examines the endocrine alterations associated with early fetal loss (EFL) induced by an environmental toxin, TCDD (2,3,7, 8-tetrachlorodibenzo-p-dioxin), in the cynomolgus macaque, a well-documented reproductive/developmental model for humans. Females were administered single doses of 1, 2, and 4 microgram/kg TCDD (n = 4 per dose group) on gestational day (GD) 12. Urinary estrogen metabolites (estrone conjugates) were monitored to establish the day of ovulation, and serum hormones (estradiol, progesterone, chorionic gonadotropin, relaxin) were measured to assess ovarian and placental endocrine status before and after treatment. EFL occurred between GDs 22 and 32 in 10 of the 12 animals treated with TCDD. The primary endocrine alterations associated with TCDD treatment were significant decreases in serum estradiol and bioactive chorionic gonadotropin concentrations (p < 0.02). Less pronounced decreases in serum progesterone (p = 0.10) and relaxin (p < 0.08) also followed TCDD treatment. In contrast, immunoreactive chorionic gonadotropin concentrations were not reduced by TCDD exposure at any level, indicating that TCDD targets specific components of the chorionic gonadotropin synthesis machinery within the trophoblast to alter the functional capacity of the hormone. These data demonstrate the value of endocrine biomarkers in identifying a toxic exposure to primate pregnancy many days before direct signs of reproductive toxicity were apparent. The increased EFL that occurred after exposure to TCDD might reflect a toxic response initially mediated via endocrine imbalance, leading to placental insufficiency, compromised embryonic circulation, and subsequent EFL.  (+info)

Oesophageal epithelial innervation in health and reflux oesophagitis. (7/39254)

BACKGROUND: The response of the oesophagus to refluxed gastric contents is likely to depend on intact neural mechanisms in the oesophageal mucosa. The epithelial innervation has not been systematically evaluated in health or reflux disease. AIMS: To study oesophageal epithelial innervation in controls, and also inflamed and non-inflamed mucosa in patients with reflux oesophagitis and healed oesophagitis. PATIENTS: Ten controls, nine patients with reflux oesophagitis, and five patients with healed oesophagitis. METHODS: Oesophageal epithelial biopsy specimens were obtained at endoscopy. The distribution of the neuronal marker protein gene product 9.5 (PGP), and the neuropeptides calcitonin gene related peptide (CGRP), neuropeptide Y (NPY), substance P (SP), and vasoactive intestinal peptide (VIP) were investigated by immunohistochemistry. Density of innervation was assessed by the proportion of papillae in each oesophageal epithelial biopsy specimen containing immunoreactive fibres (found in the subepithelium and epithelial papillae, but not penetrating the epithelium). RESULTS: The proportion of papillae positive for PGP immunoreactive nerve fibres was significantly increased in inflamed tissue when compared with controls, and non-inflamed and healed tissue. There was also a significant increase in VIP immunoreactive fibres within epithelial papillae. Other neuropeptides showed no proportional changes in inflammation. CONCLUSIONS: Epithelial biopsy specimens can be used to assess innervation in the oesophagus. The innervation of the oesophageal mucosa is not altered in non-inflamed tissue of patients with oesophagitis but alters in response to inflammation, where there is a selective increase (about three- to fourfold) in VIP containing nerves.  (+info)

Elevated carboxy terminal cross linked telopeptide of type I collagen in alcoholic cirrhosis: relation to liver and kidney function and bone metabolism. (8/39254)

BACKGROUND: The carboxy terminal cross linked telopeptide of type I collagen (ICTP) has been put forward as a marker of bone resorption. Patients with alcoholic liver disease may have osteodystrophy. AIMS: To assess circulating and regional concentrations of ICTP in relation to liver dysfunction, bone metabolism, and fibrosis. METHODS: In 15 patients with alcoholic cirrhosis and 20 controls, hepatic venous, renal venous, and femoral arterial concentrations of ICTP, and bone mass and metabolism were measured. RESULTS: Circulating ICTP was higher in patients with cirrhosis than in controls. No overall significant hepatic disposal or production was found in the patient or control groups but slightly increased production was found in a subset of patients with advanced disease. Significant renal extraction was observed in the controls, whereas only a borderline significant extraction was observed in the patients. Measurements of bone mass and metabolism indicated only a mild degree of osteodystrophy in the patients with cirrhosis. ICTP correlated significantly in the cirrhotic patients with hepatic and renal dysfunction and fibrosis, but not with measurements of bone mass or metabolism. CONCLUSIONS: ICTP is highly elevated in patients with cirrhosis, with no detectable hepatic net production or disposal. No relation between ICTP and markers of bone metabolism was identified, but there was a relation to indicators of liver dysfunction and fibrosis. As the cirrhotic patients conceivably only had mild osteopenia, the elevated ICTP in cirrhosis may therefore primarily reflect liver failure and hepatic fibrosis.  (+info)

TY - JOUR. T1 - Remote Blood Biomarkers of Longitudinal Cognitive Outcomes in a Population Study. AU - Rajan, Kumar B.. AU - Aggarwal, Neelum T.. AU - McAninch, Elizabeth A.. AU - Weuve, Jennifer. AU - Barnes, Lisa L.. AU - Wilson, Robert S.. AU - DeCarli, Charles. AU - Evans, Denis A.. PY - 2020. Y1 - 2020. N2 - Objective: The longitudinal association of the blood biomarkers total tau (t-tau), neurofilament light (Nf-L), and glial fibrillary acidic protein (GFAP) with common sporadic Alzheimer disease (AD) and cognitive decline is not established. Methods: Using a single molecule array technology, ultrasensitive immunoassays for serum concentrations of t-tau, Nf-L, and GFAP were measured in a population sample of 1,327 participants (60% African Americans and women) who had a clinical evaluation for AD, had completed in-home cognitive assessments, and had undergone 1.5T structural magnetic resonance imaging. Results: Higher concentrations of serum biomarkers were associated with the development ...
Using published dairy cattle liver transcriptomics dataset along with novel blood biomarkers of liver function, metabolism, and inflammation we have attempted an integrative systems biology approach applying the classical functional enrichment analysis using DAVID, a newly-developed Dynamic Impact Approach (DIA), and an upstream gene network analysis using Ingenuity Pathway Analysis (IPA). Transcriptome data was generated from experiments evaluating the impact of prepartal plane of energy intake [overfed (OF) or restricted (RE)] on liver of dairy cows during the peripartal period. Blood biomarkers uncovered that RE vs. OF led to greater prepartal liver distress accompanied by a low-grade inflammation and larger proteolysis (i.e., higher haptoglobin, bilirubin, and creatinine). Post-partum the greater bilirubinaemia and lipid accumulation in OF vs. RE indicated a large degree of liver distress. The re-analysis of microarray data revealed that expression of ,4,000 genes was affected by diet x ...
TY - JOUR. T1 - Buccal Cell Cytokeratin 14 Correlates with Multiple Blood Biomarkers of Alzheimers Disease Risk. AU - Leifert, Wayne R.. AU - Nguyen, Tori. AU - Rembach, Alan. AU - Martins, Ralph. AU - Rainey-Smith, Stephanie. AU - Masters, Colin L.. AU - Ames, David. AU - Rowe, Christopher C.. AU - Macaulay, S. Lance. AU - François, Maxime. AU - Fenech, Michael F.. AU - The AIBL Research Group. PY - 2015/9/9. Y1 - 2015/9/9. N2 - Mild cognitive impairment (MCI) may reflect early stages of neurodegenerative disorders such as Alzheimers disease (AD). Our hypothesis was that cytokeratin 14 (CK14) expression could be used with blood-based biomarkers such as homocysteine, vitamin B12, and folate to identify individuals with MCI or AD from the Australian Imaging, Biomarkers and Lifestyle (AIBL) flagship study of aging. Buccal cells from 54 individuals were analyzed by a newly developed method that is rapid, automated, and quantitative for buccal cell CK14 expression levels. CK14 was negatively ...
Researchers at the University of Glasgows Institute of Health and Wellbeing have announced they have found people with genetic risk of Alzheimers disease exhibiting clear differences in certain biomarkers.. It supports a link between a major genetic risk factor and blood cholesterol levels. The risk factor APOE e4, which is present in a quarter of the population and triples the risk of. dementia, could possibly be the key to future early diagnosis and treatment. The 2% of the. population with a double copy have a 15 times higher risk of developing the disease.. The team investigated associations between common blood biomarkers and apolipoprotein E (APOE), a known genetic risk factor for Alzheimers disease, to improve understanding of disease mechanisms and risk. The study reviewed anonymised data from nearly 400,000 White European participants of the UK Biobank, a public health database, including lifestyle data in their analysis to account for the impact of environmental factors. Using a ...
Hip fracture represents a substantial acute inflammatory trauma, which may constitute a significant insult to the degenerating brain. Research suggests that an injury of this kind can affect memory and thinking in the future but it is unclear whether, and how, inflammatory trauma injures the brain. The impact of Acute SystematiC inflammation upon cerebRospinal fluId and blood BiomarkErs of brain inflammation and injury in Dementia: a study in acute hip fracture patients (ASCRIBED) explores this relationship, to understand the effect of inflammation on the progression of dementia. This protocol describes a multi-centre sample collection observational study. The study utilises the unique opportunity provided by hip fracture operations undertaken via spinal anaesthesia to collect cerebrospinal fluid (CSF) and blood, to investigate the impact of acute brain inflammation caused by hip fracture on the exacerbation of dementia. We will recruit 200 hip fracture patients with a diagnosis or evidence of dementia;
The ARIC (Atherosclerosis Risk in Communities) study recruited a population-based cohort of 15,792 persons between 45 and 64 years of age in 1987-1989, from Forsyth County, NC, and Jackson, MS. In the ARIC study cohort, the investigators measured multiple blood biomarkers of inflammation, hemostasis, thrombin generation, cardiac dysfunction, and vascular stiffness, and identified incident AAAs during follow-up using hospital discharge codes. The authors performed multivariate modeling to obtain hazard ratios of incident clinical AAA in relation to biomarker quartiles by use of Cox proportional hazards regression.. ...
This trial will investigate the effiects of nintedanib [OFEV; Boehringer Ingelheim] on chnages in specific blood biomarkers that can predict course of
The pathogenesis of HIV infection is highly complex and involves numerous actors of the immune system. On the one hand, our immunity has a predominant role in limiting HIV replication and the depletion of its targets, but on the other hand, the persistent infection established by the virus is associated with chronic immune activation and inflammation, potentially resulting in the progressive exhaustion of the host immune resources, and in the onset of non-AIDS-defining comorbidities. The thorough study of HIV pathogenesis is increasingly more challenging. New knowledge together with technological advances offers the possibility to monitor a constellation of cellular immune markers. Here, we discuss the relevance of studying these markers in order to assess the efficacy to control HIV, the inflammatory response to HIV infection, and the alteration and exhaustion of the immune compartments. Monitoring these cellular immune markers is important to reach a deeper understanding of HIV pathogenesis ...
TY - JOUR. T1 - Correction to: Amyloid-β misfolding as a plasma biomarker indicates risk for future clinical Alzheimers disease in individuals with subjective cognitive decline (Alzheimers Research & Therapy, (2020), 12, 1, (169), 10.1186/s13195-020-00738-8). T2 - Amyloid-β misfolding as a plasma biomarker indicates risk for future clinical Alzheimers disease in individuals with subjective cognitive decline (Alzheimers Research & Therapy, (2020), 12, 1, (169), 10.1186/s13195-020-00738-8). AU - Stockmann, Julia. AU - Verberk, Inge M. W.. AU - Timmesfeld, Nina. AU - Denz, Robin. AU - Budde, Brian. AU - Lange-Leifhelm, Julia. AU - Scheltens, Philip. AU - van der Flier, Wiesje M.. AU - Nabers, Andreas. AU - Teunissen, Charlotte E.. AU - Gerwert, Klaus. PY - 2021/12/1. Y1 - 2021/12/1. N2 - Following publication of the original article [1], the authors noticed that the published figures have errors which was occurred during processing of the figures in production team. 1) fonts are shifted ...
In this post-hoc analysis of a subcohort of a large, multicenter prospective cohort study of cardiac surgery patients without creatinine-based acute kidney injury, we did not find evidence of association between either pre- or postoperative biomarker levels and age or sex. However, there were significant differences in biomarker levels associated with urine dipstick findings, including proteinuria, hematuria, leukocyte esterase and nitrites.. All biomarkers were mildly correlated with hematuria, indicating that blood might interfere with biomarker levels or their measurement even in the absence of overt kidney injury evidenced by creatinine rise. Preexisting substances in the urine interfering with assays for novel analytes has been previously described. [10]. We found evidence that proteinuria is associated with all four biomarkers both pre- and post-operatively. Dipstick proteinuria is a marker of preexisting kidney damage and is associated with both the incidence and outcomes of acute kidney ...
Cardiac markers are biomarkers measured to evaluate heart function. They are often discussed in the context of myocardial infarction, but other conditions can lead to an elevation in cardiac marker level. Most of the early markers identified were enzymes, and as a result, the term cardiac enzymes is sometimes used. However, not all of the markers currently used are enzymes. For example, in formal usage, troponin would not be listed as a cardiac enzyme. Measuring cardiac biomarkers can be a step toward making a diagnosis for a condition. Whereas cardiac imaging often confirms a diagnosis, simpler and less expensive cardiac biomarker measurements can advise a physician whether more complicated or invasive procedures are warranted. In many cases medical societies advise doctors to make biomarker measurements an initial testing strategy especially for patients at low risk of cardiac death. Many acute cardiac marker IVD products are targeted at nontraditional markets, e.g., the hospital ER instead ...
The results of this study show that changes in numbers of synovial sublining macrophages correlate with clinical improvement independently of the therapeutic strategy. Furthermore, this study demonstrates that the change in the numbers of sublining macrophages may be used to explain clinical outcome. Of importance, the data indicate that the change in the number of sublining macrophages could be used as a sensitive biomarker to predict possible efficacy of a new antirheumatic treatment.. Previous work suggested an association between the number of synovial macrophages and joint destruction in RA.14 Moreover, analysis of the synovial cell infiltrate demonstrated a positive correlation between scores for local disease activity and the number of macrophages as well as expression of macrophage derived cytokines (tumour necrosis factor α (TNFα) and interleukin (IL) 6) in rheumatoid ST, suggesting that macrophage numbers are associated with clinical signs of inflammation.2 In keeping with this ...
SirYus technology enables sensitive and reproducible tests for cancer screening by combining several biomarkers to establish an appropriate diagnosis.. Considerable efforts have been devoted to search specific markers with relatiely few results. In fact, from 1261 proteins identified as potentially discriminatory in human cancers, only nine have received final approval from the FDA as biomarkers of tumors.. Many opinion leaders now believe that the pursuit of individual biomarkers will be vain, and that several biomarkers are needed to properly identify cancers in screening protocols.. SirYus multiplex technology brings new solutions to this complex challenge. Simultaneous testings of several biomarkers can largely improve screening sensitivity and specificity.. ...
In clinical practice, biomarkers can be used to identify risk and susceptibility, diagnose a disease, assess disease severity or progression, classify patients, guide treatment, and predict prognosis. In drug development and the pharmaceutical industry, biomarkers can be used to predict toxicity, safety, or efficacy of a drug. Biomarkers can be categorized as target, mechanism and clinical to indicate if a drug hits its intended target, alters any mechanisms and if it is effective in vivo. Biomarkers can be also classified into three types: type 0 - natural history markers, type 1 - biological or drug activity markers, and type 2 - surrogate markers. Type 0 biomarkers measure the natural history of a disease and should correlate over time with known clinical indicators. They can be characterized in phase 0 clinical trials. Symptoms over the full range of a disease and most prognosis markers are type 0 biomarkers. In most cases, type 1 biomarkers are the markers that capture the effects of a ...
With demographic shifts toward older populations, the number of people with dementia is steadily increasing. Alzheimers disease (AD) is the most common cause of dementia, and no curative treatment is available. The current best strategy is to delay disease progression and to practice early intervention to reduce the number of patients that ultimately develop AD. Therefore, promising novel biomarkers for early diagnosis are urgently required. To identify blood-based biomarkers for early diagnosis of AD, we performed RNA sequencing (RNA-seq) analysis of 610 blood samples, representing 271 patients with AD, 91 cognitively normal (CN) adults, and 248 subjects with mild cognitive impairment (MCI). We first estimated cell-type proportions among AD, MCI, and CN samples from the bulk RNA-seq data using CIBERSORT and then examined the differentially expressed genes (DEGs) between AD and CN samples. To gain further insight into the biological functions of the DEGs, we performed gene set enrichment analysis (GSEA
CSDE is offering a hands-on workshop in biomarker methods that provide quick on-site measurements in non-clinical settings without the need to transport samples back to a lab. Well practice proper technique for minimally invasive measures like height, weight, body composition, and blood pressure, and the use of simple tools for biomarker testing with blood collected from a finger prick. A brief lecture component will cover costs, logistics, human subjects implications, and strengths and limitations of this approach to biomarker data collection. Please email Ellie Brindle ([email protected]) to register.. ...
Dive into the research topics of An analysis of available biomarker data for targeting cyclin-dependent kinases 4 and 6 (CDK4/6) in breast cancer. Together they form a unique fingerprint. ...
Attempts to find a lone biomarker for Alzheimers disease - whether its in blood, spinal fluid, or the brain - have largely failed. The Texas Alzheimers Research Consortium project conducted a longitudinal case-control study, using stored blood samples to develop an algorithm that separates patients with Alzheimers disease from controls. The biomarker assays looked at hundreds of proteins, including thrombopoietin, TNF-alpha, creatine kinase, and various interleukins. The his team focused on a large array of blood-based proteins, since assay technology has now made it possible to evaluate large amounts of data. Screening for these biomarkers and factoring in age, sex, education, and APOE status led to a sensitivity of 0.94 and a specificity of 0.84, as reported by Sid OBryant, PhD, of Texas Tech University, in Lubbock, Texas, and colleagues in the Archives of Neurology. They also saw that many of the proteins with the highest importance were inflammatory in nature, which suggests that the ...
Background: Metabolic alterations correlate with adverse outcomes in type 2 diabetes. Dietary modification serves as an integral part in its treatment.. Objective: We examined the relationships among dietary patterns, dietary biomarkers, and metabolic indicators in type 2 diabetes (n = 871).. Design: Diabetic patients (n = 871) who provided complete clinical and dietary data in both 2008 and 2009 were selected from a cohort participating in a diabetic control study in Taiwan. Dietary data were obtained using a short, semiquantitative food frequency questionnaires, and dietary pattern identified by factor analysis. Multiple linear regressions were used to analyze the association between dietary biomarkers (ferritin, folate, and erythrocyte n-3 polyunsaturated fatty acids [n-3 PUFAs]) and metabolic control upon adjusting for confounders.. Results: Three dietary patterns (high-fat meat, traditional Chinese food-snack, and fish-vegetable) were identified. Ferritin correlated positively with high-fat ...
In this study, biomarker response after supplementation with oral and intramuscular vitamin B12 will be compared in a randomized clinical trial. Electronic compliance monitoring will be used to control for non compliance as a possible confounder in oral treatment. Additionally subjective acceptance in terms of presumed preferences will be compared with oral vs. intramuscular supplementation of vitamin B12 in the view of the patient ...
A diagnostic study of 136 premature infants found that a protein involved in managing harmful bacteria in the human intestine is a reliable biomarker for the noninvasive detection of necrotizing enterocolitis.
Purdue University researchers have found a method of identifying biological markers in small amounts of blood that they believe could be used to detect a myriad of diseases, infections and different medical conditions at early stages.
Hypothesis: The association of excess weight with an adverse cardiometabolic profile in patients with type 1 diabetes (T1D) is unclear. The purpose of this study was to test the hypothesis that increasing BMI and adiposity indicators in youth with T1D are adversely associated with glycemic control and cardiovascular biomarkers.. Methods: Subjects were youth participants of a family-based randomized controlled dietary intervention (N=136, age=12.3±2.5y, baseline A1c=8.1±1.1%). Glycemic control (A1c and 1,5-Anhydroglucitol, 1,5-Ag), body mass index (BMI, from measured height and weight), serum lipids (total cholesterol, TC; HDL-cholesterol, HDL-C; LDL-cholesterol, LDL-C; triglycerides, TG), inflammation (c-reactive protein, CRP), oxidative stress (8-iso-prostaglandin F2alpha, 8-iso-PGF2α), adiponectin and blood pressure (systolic, SBP; diastolic, DBP) were assessed at baseline and every 6 months for 18 months. Total and truncal lean, fat-free mass and percent fat (%fat) were measured by Dual ...
Nivolumab response rate is 40% in metastatic melanoma. Few studies have evaluated pre-treatment biomarkers predictive of response. The aim of this study was to identify potential peripheral blood biomarkers associated with survival in patients with advanced melanoma treated with nivolumab. All advanced melanoma cases treated with anti-programmed cell death protein 1 (anti-PD1) over a 3-year period in our department were identified. For each case, 9 potential blood biomarkers were identified. Bivariate and multivariate analyses, adjusted for the American Joint Committee on Cancer (AJCC) classification stage, Eastern Cooperative Oncology Group (ECOG) performance status, lactate dehydrogenase (LDH) level and failure to respond to first-line therapy, were used to test the association between biomarkers and overall survival (primary outcome) or progression-free survival (secondary outcome ...
Using machine learning tools to analyze hundreds of proteins, UT Southwestern researchers have identified a group of biomarkers in blood that could lead to an earlier diagnosis of children with autism spectrum disorder (ASD) and, in turn, more effective therapies sooner.
Biomarkers in the bloodstream could provide a rapid assessment of therapeutic intervention in ischemic stroke and allow patient stratification Researc
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy
Non-invasive biomarkers in NASH are beginning to show where and how they can upstage liver biopsies, with the first impact appearing in patient recruitment.
ESMO) 2016 Congress. The presentation included interim efficacy and safety outcomes, as well as initial findings from the companys ongoing biomarker analysis of samples collected during the study. The SUNRISE Phase III trial was discontinued earlier this year based on a pre-specified interim analysis although patient treatment and follow-up in the study were allowed to continue. The pre-planned biomarker analysis has been taking place as patient follow-up has continued and available results were evaluated as part of the recent top-line data analysis. The study protocol pre-specified the collection of thousands of patient samples for exploratory analyses over a wide range of possible biomarkers, including pre-treatment levels of beta-2 glycoprotein-1 (β2GP1). Data presented at ESMO demonstrated that patients with pre-treatment β2GP1 levels between 200 and 240 (representing approximately 30% of randomized patients) achieved a statistically significant, 5.5-month improvement (13.2 months vs. 7.7 ...
Our literature search identified 93 studies evaluating an overall of 70 different blood markers for CRC, including long discussed ones like carcinoembryonic antigen and carbohydrate antigens, as well as newly introduced ones like proteins identified through MS analysis. A broad timeframe with first studies from 1979 (75) to 2006 (49, 73, 74, 77, 81, 89-91, 102) was covered. The majority of studies evaluated protein markers, but in recent years, an interesting number of studies also evaluated genetic and epigenetic markers.. Overall, a broad range of sensitivity and specificity was reported for the various markers. A direct comparison of results from different studies is complicated due to the diverse populations used (different age, origin, normal, or diseased controls), the diverse number of markers evaluated (single versus combined markers) and use of different cutoff points for the same marker (M2-PK and CEA). Furthermore, the majority of markers were evaluated in only one study, especially ...
Eglinton, Geoffrey (2004): Lipid biomarkers at station CD47_0206S#16. PANGAEA,, In: Lowry, Roy K; Machin, P (2016): Compilation of the results of EU-project BOFS. PANGAEA,
Alzheimer disease biomarker testing in cerebrospinal fluid : a method to harmonize assay platforms in the absence of an absolute reference standard ...
Researchers with eyes peeled toward prodromal Alzheimers disease probably see this diagram in their sleep-the one where the fields five most validated markers trace their poignant path from normal cognition to fully developed AD. Two research groups proposed this theoretical model some years ago (see Perrin et al., 2009; Jack et al., 2010). But is it true? At least in its broad strokes, the answer seems to be yes, according to early data pouring in from biomarker studies in autosomal-dominant AD. By and large, brain amyloid, brain metabolism, atrophy, and functional connectivity data from independent analyses of the Dominantly Inherited Alzheimer Network (DIAN) and the Alzheimers Prevention Initiative (API) jibe with each other and confirm the sequence of preclinical biomarker changes proposed for late-onset AD. This article highlights DIAN and API neuroimaging results reported 14-19 July 2012 at the Alzheimers Association International Conference (AAIC) in Vancouver, Canada.. DIAN and API ...
Serological and proteomic biomarkers can help clinicians diagnose rheumatic diseases earlier and assess disease activity more accurately. These markers have been incorporated into the recently revised classification criteria of several diseases to enable early diagnosis and timely initiation of treatment. Furthermore, they also facilitate more accurate subclassification and more focused monitoring for the detection of certain disease manifestations, such as lung and renal involvement. These biomarkers can also make the assessment of disease activity and treatment response more reliable. Simultaneously, several new serological and proteomic biomarkers have become available in the routine clinical setting-for example, a protein biomarker panel for rheumatoid arthritis and a myositis antibody panel for dermatomyositis and polymyositis. This review will focus on commercially available antibody and proteomic biomarkers in rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis ...
Serum cytokeratin-18 is a non-invasive biomarker for evaluating disease severity in patients with liver cirrhosis and hepatocellular carcinoma
Semantic Scholar extracted view of Dynamic changes in calprotectin and its correlation with traditional markers of oxidative stress in patients with acute ischemic stroke. by Antonios Chatzopoulos et al.
Biomarker classification depends on its context of use, which in turn defines the extent to which the biomarker must be validated. In terms of requirements for validation, the holy grail of biomarker application is one that can serve as a surrogate endpoint in a clinical trial. A surrogate endpoint is a laboratory (or physical) measurement that is used in a clinical trial as a substitute for a clinical endpoint (e.g., survival or functional endpoint). Therefore, a surrogate endpoint must accurately predict the effect of therapeutic intervention. Guidance for Industry: Pharmacogenomic Data Submissions. The use of companion diagnostics to drive molecularly targeted drug development also requires that the diagnostic be clinically validated through the FDAs PMA or 510(K) process. In both of these instances the biomarker must be analytically validated (inclusive of the test platform, reagents, etc.).. The subtle differences between biomarkers, diagnostics, surrogate endpoints and companion ...
This study has been designed to evaluate a new oral test for therapeutic monitoring of HIV/AIDS patients that are receiving the combination Anti-Retroviral Therapy (cART). The test will measure saliva-based Stress Response Profiling(SRP) biomarkers using laboratory assays. Results of the test will show if HIV/AIDS patients successfully responded to cART. Preliminary studies showed that SRP biomarkers were strongly increased in cART-unresponsive AIDS patients. However, the diagnostic accuracy of the oral test, patients will be recruited to donate saliva: AIDS patients responsive or unresponsive to cART, and controls (acute or early HIV patients, and HIV-negative patients with hepatitis).. The saliva samples will be used to measure SRP biomarker concentrations. Results will show whether the biomarker measurements provide accurate and specific diagnostics for ART response. ...
Below is a brief summary of dates of CSF biomarker analyses run as batches of samples using the Luminex xMAP platform and Innogenetics INNO-BIA AlzBio3? immunoassay reagents. Normalization of CSF biomarker data between results of 2007 and 2008 have been obtained using a statistical approach, resulting in a procedure to maintain lot-to-lot consistency within clinical studies. In addition, during the further review of the pTau181p data a subset of CSF samples required quaranteening all pTau181p data from the late 2008 batch run. The normalization applies to all t-tau, A?1-42 and pTau181p results, with the exception of the subset of pTau181p results that require a special correction procedure that is in the process of being completed. That specific subset of data will be uploaded in the near future.. Batch runs of ADNI CSF samples ...
This paper addresses the problem of classifying cells expressing different biomarkers. A deep learning based method that can automatically localize and count the cells expressing each of the different biomarkers is proposed. To classify the cells, a Convolutional Neural Network (CNN) was employed. Images of Immunohistochemistry (IHC) stained slides that contain these cells were digitally scanned. The images were taken from digital scans of IHC stained cervical tissues, acquired for a clinical trial. More than 4,500 RGB images of cells were used to train the CNN. To evaluate our method, the cells were first manually labeled based on the expressing biomarkers. Then we performed the classification on 156 randomly selected images of cells that were not used in training the CNN. The accuracy of the classification was 92% in this preliminary data set. The results have shown that this method has a good potential in developing an automatic method for immunohistochemical analysis ...
Cancer biomarkers are the measurable molecular changes to either cancerous or normal tissues of patients. Although the term biomarker most commonly refers to altered expression of certain gene products or abnormal DNA configurations, changes to cellular processes such as energy metabolism and DNA damage response can also be used as biomarkers in a broader sense. Cancer biomarkers have multiple implications in cancer intervention. A reliable biomarker can be used for cancer diagnosis, risk and prognosis assessments, and for the surveillance of treatment effectiveness. More importantly, some, but not all, biomarkers can be exploited as therapeutic targets. This is because some biomarkers may be simply messengers that do not directly contribute to the tumor growth and are thus not ideal therapeutic targets. Only the driver or conspirator biomarkers that directly contribute to tumor growth may be targeted for therapy. Therefore, effort in the development of targeted therapies must not simply ...
Fingerprint Dive into the research topics of Level of dietary energy and 2,4-thiazolidinedione alter molecular and systemic biomarkers of inflammation and liver function in Holstein cows. Together they form a unique fingerprint. ...
Normally distributed continuous variables were compared with the Student t test and skewed-distributed with the Wilcoxon rank-sum test. Categorical variables were compared with the chi-square test. The prognostic value of the biomarkers was assessed by investigating the relationship between mortality and biomarkers in 2 sets of Cox proportional-hazards analyses: univariate analyses of single biomarkers and multivariate analysis including all biomarkers (multimarker). The univariate analyses were used to determine cutoff values for the biomarkers. These cutoff values were based on the quartiles of the individual biomarker levels. When hazard did not differ significantly between adjacent quartiles, as indicated by the p value from the Cox model, these were merged.. A multivariate model initially was developed incorporating the 5 biomarkers. Biomarkers with p < 0.05 by the Wald test were identified by backward selection. From the final model, a simplified score was obtained by putting weights to ...
Learn about the veterinary topic of Other Serum Biochemical Measures in Hepatic Disease in Small Animals. Find specific details on this topic and related topics from the Merck Vet Manual.
Press Release issued Jul 8, 2014: Biomarkers also known as biological markers are characteristics that are measured in order to assess and predict biological condition of the body and thus help physicians in clinical decision making. Some major diseases which are diagnosed by using biomarkers include diabetes, cancer and cardiovascular and central nervous system diseases. Biomarkers play critical role in identifying and accurate diagnosis of these diseases at early stage and provide better treatment option. Imaging biomarkers can be classified as diagnostic biomarkers, monitoring biomarkers and predictive biomarkers. Diagnostic biomarkers are helpful in predicting a patients exact clinical outcome. Monitoring biomarkers provides clinical outcome of a patient after intake of a particular drug while predictive biomarkers help in predicting if a specific drug is likely to change clinical outcome of a patient.
The dependence of tumor growth and metastasis on blood vessels makes tumor angiogenesis a rational target for therapy. Strategies have been pursued to inhibit neovascularization and to destroy existing tumor vessels, or both. These include direct targeting of endothelial cells, and indirect targeting by inhibiting the release of proangiogenic growth factors by cancer or stromal cells. Many patients benefit from antiangiogenic therapies; thus, development of noninvasive biomarkers of disease response and relapse is a crucial objective to aid in their management. A number of non-invasive tools are described with their potential benefits and limitations. We review currently available candidate biomarkers of anti-angiogenic agent effect. Including these markers into clinical trials may provide insight into appropriate dosing for desired biological effects, appropriate timing of additional therapy, and prediction of individual response. This has important consequences for the clinical use of angiogenesis
The objective of the project is to validate a serum biomarker signature for the early diagnosis (yes/no diagnosis) of pancreatic cancer...
The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes
In [11], we first proposed an efficient algorithm, AMFES (Adaptive Multiple FEatues Selection), to select important biomarkers for cancers. Based on that initial success, this paper reports the extension of previous results on the datasets provided by Maes et al. in an attempt to discover important biomarkers for AD from the blood-based samples [12]. Unlike traditional statistical analyses, AMFES is an SVM-based methodology, which can select a much smaller subset of important biomarkers. In addition, AMFES applies an adaptive method which enables selection of a globally optimal subset of important biomarkers compared to SVM-RFE. AMFES is particularly useful for differentiating noisy biomarkers from the relevant ones when interferences between biomarkers exist. Our results are supported by a high ROC/AUC (Receiver Operating Characteristic/Area Under Curve) value when we apply a cross-validation verification. Thus, AMFES should play an important role in the classification framework of ...
BACKGROUND: In patients with end-stage renal disease (ESRD), the ability of single and multiple biomarker monitoring to predict adverse outcomes has not been well established. This study determined the prognostic value of multiple biomarkers for all-cause death over 2 years in 399 ESRD patients.. METHODS: The risk of all-cause death was determined by use of multiple biomarkers based on concentrations for a reference population (normal) and cutoffs based on tertile distributions in the ESRD group. Biomarkers studied included N-terminal pro-B-type natriuretic peptide (NT-proBNP), high-sensitivity C-reactive protein (hsCRP; Dade Behring and Roche assays), and cardiac troponin T (cTnT; Roche) and I (cTnI; Dade Behring and Beckman Coulter assays). Relative risks of death were estimated and survival curves computed.. RESULTS: A total of 101 deaths occurred during 594 patient-years of follow-up. Increased NT-proBNP concentrations were not predictive of death on the basis of the normal cutoffs. However, ...
Background Linifanib is a potent and selective VEGF and PDGF receptor inhibitor that has activity in unselected, advanced NSCLC patients (pts) both as monotherapy in the relapsed setting and with carboplatin (C) and paclitaxel (P) in the first-line setting. A baseline plasma biomarker signature identifying NSCLC pts most sensitive to linifanib is needed.. Methods An exploratory retrospective analysis of 4 randomized clinical trials (linifanib or other treatments: ABT-510 [thrombospondin mimetic], pemetrexed +/- ABT-751 [tubulin inhibitor], docetaxel +/- ABT-751) in relapsed NSCLC was conducted. Evaluable baseline plasma samples were obtained from 116 pts who received linifanib and 125 pts on other treatments. A signature combining established tumor markers (carcinoembryonic antigen [CEA] and fragments of cytokeratin 19 [CYFRA 21-1]) was derived using a sequential BATTing approach. The signature was then tested across a randomized trial of CP + placebo, linifanib 7.5 mg, or linifanib 12.5 mg in ...
1 Industry Overview. 1.1 Definition and Specifications of Molecular Biomarkers for Cancer Detection and Management. 1.2 Classification of Molecular Biomarkers for Cancer Detection and Management. 1.3 Applications of Molecular Biomarkers for Cancer Detection and Management. 1.4 Industry Chain Structure of Molecular Biomarkers for Cancer Detection and Management. 1.5 Industry Overview and Major Regions Status of Molecular Biomarkers for Cancer Detection and Management. 1.5.2 Global Major Regions Status of Molecular Biomarkers for Cancer Detection and Management. 1.6 Industry Policy Analysis of Molecular Biomarkers for Cancer Detection and Management. 1.7 Industry News Analysis of Molecular Biomarkers for Cancer Detection and Management. 2 Manufacturing Cost Structure Analysis of Molecular Biomarkers for Cancer Detection and Management. 2.1 Raw Material Suppliers and Price Analysis of Molecular Biomarkers for Cancer Detection and Management. 2.2 Equipment Suppliers and Price Analysis of Molecular ...
Our data indicate first that among a panel of 10 biomarkers, plasma Nt-proBNP level predicts future fatal and nonfatal cardiac events and adds significantly to the information obtained from determination of traditional risk factors in individuals with existing CVD. Second, although various inflammatory biomarkers and microalbuminuria were individually significantly related to future cardiovascular risk, they added very little additional prognostic information to the traditional markers. Third, the present data illustrate that models that include simple and readily available risk factors do not gain accuracy by the inclusion of any inflammatory biomarker for prediction of cardiovascular events in this setting of preexisting disease.. An increasing number of biomarkers have been proposed to identify patients at high risk for future cardiovascular events in the setting of primary3-6,19 and secondary7-9,20 prevention, as well as acute coronary syndrome.10,21-25 From a clinical perspective, the ...
TY - JOUR. T1 - Clinical utility of NT-proBNP, a new biomarker of cardiac function and heart failure. AU - Ishii, Junichi. PY - 2008/4. Y1 - 2008/4. N2 - Recently, N-terminal pro-B-type natriuretic peptide (NT-proBNP), a new biomarker of cardiac function and heart failure, has become available as a clinical laboratory test in Japan. Its diagnostic and prognostic utility appears to be equivalent to BNP in the clinical setting; however, there are some biologic differences between NT-proBNP and BNP. Unlike BNP, NT-proBNP is not degraded in the circulation, and is stable even in serum. It has a longer half-life of approximately 1 to 2 hours, leading to higher circulating levels and slower fluctuations than BNP. Both of these biomarkers are influenced by renal function, but the effect is greater for NT-proBNP. Recently, NT-proBNP has become an important diagnostic tool for assessing patients who present acutely with dyspnea, and provides important prognostic information in both acute and chronic ...
Urine samples were collected between 2002 and 2006 among 287 pregnant women from Eden and Pélagie cohorts, from which phthalates and phenols metabolites levels were assayed. We applied a 2-step standardization method based on regression residuals. First, the influence of sampling conditions (including sampling hour, duration of storage before freezing) and of creatinine levels on biomarker concentrations were characterized using adjusted linear regression models. In the second step, the model estimates were used to remove the variability in biomarker concentrations due to sampling conditions and to standardize concentrations as if all samples had been collected under the same conditions (e.g., same hour of urine collection ...
TY - JOUR. T1 - Cerebrospinal fluid and plasma biomarker trajectories with increasing amyloid deposition in Alzheimers disease. AU - Palmqvist, Sebastian. AU - Insel, Philip S.. AU - Stomrud, Erik. AU - Janelidze, Shorena. AU - Zetterberg, Henrik. AU - Brix, Britta. AU - Eichenlaub, Udo. AU - Dage, Jeffrey L.. AU - Chai, Xiyun. AU - Blennow, Kaj. AU - Mattsson, Niklas. AU - Hansson, Oskar. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Failures in Alzheimers disease (AD) drug trials highlight the need to further explore disease mechanisms and alterations of biomarkers during the development of AD. Using cross-sectional data from 377 participants in the BioFINDER study, we examined seven cerebrospinal fluid (CSF) and six plasma biomarkers in relation to β-amyloid (Aβ) PET uptake to understand their evolution during AD. In CSF, Aβ42 changed first, closely followed by Aβ42/Aβ40, phosphorylated-tau (P-tau), and total-tau (T-tau). CSF neurogranin, YKL-40, and neurofilament light increased after the ...
Different classes of cardiovascular biomarkers are thought to provide information concerning different pathophysiological mechanisms.16 Because anthracycline therapy is associated with both cardiomyocyte injury, loss of cardiac contractile function, inflammation, and development of diffuse fibrosis,17 we selected biomarkers that are believed to reflect these processes in our study.. Cardiac troponins are markers of cardiomyocyte injury and are associated with risk for cardiovascular death and heart failure.18 Moreover, the use of high‐sensitivity assays for cTnI and cTnT also permits detection and monitoring of low‐grade, chronic myocardial injury.19 BNP and NT‐proBNP are associated with cardiac function and provide strong prognostic information across the spectrum of cardiovascular disease.20, 21, 22 CRP is a prototypical inflammatory biomarker that has been associated with the incidence of cardiovascular disease and death, both in the general population, in patients with coronary artery ...
The high sensitivity C-reactive protein (hs-CRP), mediator of atherosclerotic disease, is known to be a sensitive predictor of coronary heart disease in type 2 diabetes mellitus (T2DM).. Aims: To evaluate the relationship of hs-CRP levels to metabolic syndrome (MS) and albuminuria in T2DM.. Methods: Four hundred and eighty-eight T2DM subjects were recruited. Patients with hs-CRP ≥10 mg/l, creatinine ≥1.4 mg/dl, chronic inflammatory and cardiovascular diseases, smokers or on antiplatelet therapy were excluded. In all the 178 subjects (65.2±10.7 years, 71 men and 107 women) enrolled, we measured hs-CRP, waist circumference (WC), fasting plasma glucose (FPG), systolic and diastolic blood pressure, high density lipoprotein-cholesterol (HDL-Ch), triglycerides (TGs), and 24 h. Albumin excretion (ALB-e). The MS was defined according to the IDF criteria. Student s t-test was used to compare the means, and the Mann Whitney U test to analyze the relationship between hs-CRP levels and the presence of ...
Our Canary Club High Sensitivity C-Reactive Protein (hs-CRP) blood-spot test provides an accurate assessment of the amount of inflammation in the body to provide physicians with more diagnostic clues regarding a persons cardio metabolic risk.. People who have hs-CRP levels in the high end of the normal range have a 1.5 to 4 times greater risk of having a heart attack as those in the low end of the normal range.. Measurement of hs-CRP does not reflect where in the body the inflammation is, however. It may reflect inflammation detected in cells in the fatty deposits of arterial walls, or it may reflect inflammation from any part of the body. However, when hs-CRP is tested in combination with other cardio metabolic markers, physicians can find valuable clues regarding a persons risk for type 2 diabetes, heart attack, and stroke.. Symptoms of irregular hs-CRP levels:. ...
TY - JOUR. T1 - Relation of Soft Plaque and Elevated Preprocedural High-Sensitivity C-Reactive Protein Levels to Incidence of In-Stent Restenosis After Successful Coronary Artery Stenting. AU - Hong, Young Joon. AU - Jeong, Myung Ho. AU - Lim, Sang Yeob. AU - Lee, Sang Rok. AU - Hong, Seo Na. AU - Kim, Kye Hun. AU - Sohn, Il Suk. AU - Park, Hyung Wook. AU - Kim, Ju Han. AU - Kim, Weon. AU - Ahn, Youngkeun. AU - Cho, Jeong Gwan. AU - Park, Jong Chun. AU - Kang, Jung Chaee. PY - 2006/8/1. Y1 - 2006/8/1. N2 - Although various predictors relating to in-stent restenosis (ISR) have been demonstrated, the relation between the parameters of intravascular ultrasound and inflammatory markers and ISR has not been reported. This study included 120 patients who underwent stent implantation for angiographically significant stenosis. Patients were divided into a soft plaque group (n = 50) and a nonsoft plaque group (n = 70). All patients underwent angiographic and intravascular ultrasound follow-up at 6 ...
TY - JOUR. T1 - Progress of research in osteoarthritis. The evaluation of osteoarthritis with biological markers. AU - Morita, Mitsuhiro. AU - Yamada, Harumoto. AU - Date, Hideki. AU - Yoshimura, Noriko. PY - 2009/11. Y1 - 2009/11. N2 - It is socially required to establish the correlation of some specific biomarkers and the clinical stage of osteoarthritis (OA). This study is to evaluate the usefulness of serum or urine biomarkers in OA in the large population cohort study of Japan. There are many reports to evaluate the usefulness of biological markers in OA, and the similar clinical results are supported in this study. The further follow-up study would be wished to be done.. AB - It is socially required to establish the correlation of some specific biomarkers and the clinical stage of osteoarthritis (OA). This study is to evaluate the usefulness of serum or urine biomarkers in OA in the large population cohort study of Japan. There are many reports to evaluate the usefulness of biological ...
1] Foerch C., Montaner J., Furie K.L., Ning M.M. and Lo E.H., Invited Article: Searching for oracles? Blood biomarkers in acute stroke, Neurology 73(5), 393-399 (2009). [2] Whiteley W., Tseng M.C. and Sandercock P., Blood biomarkers in the diagnosis of ischemic stroke: a systematic review, Stroke 39(10), 2902-2909 (2008). [3] Whiteley W., Chong W.L., Sengupta A. and Sandercock P., Blood markers for the prognosis of ischemic stroke: a systematic review, Stroke 40(5), 380-389 (2009). [4] Carlborg C. F., Gylfason K. B., Kaźmierczak A., Dortu F., Banuls M. J., Kresbach G. M., Sohlström H., Moh T., Vivien L., Popplewell J., Ronan G., Barrios C.A., Stemme G. and van der Wijngaart W., A packaged optical slot-waveguide ring resonator sensor array for multiplex assays in labs-on-chip, Lab on Chip 10 , 281- 290 (2010). [5] Sinclair W., Schmid J.H., Waldron P., Poitras D., Janz S., Mischki T., Lopinski G., Densmore A., Xu D.-X., Lapointe J. and Delâge A., Silicon photonic evanescent field ...
Background: Plaque rupture, acute ischemia, and necrosis in acute coronary syndromes are accompanied by concurrent pro-and anti-inflammatory cascades. Whether STEMI clinical prediction models can be improved with the addition of baseline inflammatory biomarkers remains unknown. Methods: In an APEX-AMI trial substudy, 772 patients had a panel of 9 inflammatory serum biomarkers, high sensitivity C reactive protein (hsCRP), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) measured at baseline after randomization. Baseline biomarkers were incorporated into a clinical prediction model for a composite of 90-day death, shock, or heart failure. Incremental prognostic value was assessed using Net Reclassification Improvement (NRI) and Integrated Discrimination Improvement (IDI). Results: Individually, several biomarkers were independent predictors of clinical outcome: hsCRP (hazard ratio [HR] 1.12; 95% confidence interval [CI], 1.03-1.21; p=0.007, per doubling), NT-proBNP (HR 1.14; 95% CI, ...
Diabetic kidney disease is the leading cause of end-stage renal disease in developed and developing countries. Microalbuminuria is the gold standard for detection and prediction of diabetic kidney disease and cardiovascular risk disease in clinical practice. However, microalbuminuria has several limitations, such as lower sensitive, larger variability. It is urgent to explore higher sensitivity and specificity for earlier detection of diabetic kidney disease and more accurate prediction of the progression to end stage renal disease. We reviewed some new and important urinary biomarkers, such as: transferrin, immunoglobulin G, immunoglobulin M, Cystanic C, podocytes, type IV collagen, 8-oxo-7, 8-dihydro-2-deoxyguanosine, ceruloplasmin, monocyte chemoattractant protein-1 and so on. We need good quality, long-term, large longitudinal trials to validate published biomarkers and find new biomarkers, considering biomarkers reviewed here are from small cross-sectional studies.
Amyotrophic lateral sclerosis (ALS) is a highly progressive and debilitating neurodegenerative disease, which usually leads to the death of affected individuals within a few years after the onset of symptoms. ALS is currently incurable and very little is known about its pathophysiology. Finding validated biomarkers will help us to advance our understanding of ALS etiology and find better strategies for early diagnosis and management of the disease. The main aim of the present systematic review is to evaluate the concentration of 11 frequently reported biomarkers for ALS in peripheral blood and CSF of patients diagnosed with ALS compared with controls. This systematic review protocol has been established according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocol (PRISMA-P) 2015 guideline. We will include all types of observational studies with human subjects that investigated the concentrations of intended biomarkers (amyloid beta (Aβ-42), tau and phosphorylated tau (p
Purdue University researchers have found a method of identifying biological markers in small amounts of blood that they believe could be used to detect a myriad of diseases, infections and different medical conditions at early stages.. Jeffrey Rhoads, a professor in Purdues School of Mechanical Engineering; George Chiu, a professor in Purdues School of Mechanical Engineering, School of Electrical and Computer Engineering, and Department of Psychological Sciences; and Eric Nauman, a professor in the School of Mechanical Engineering, Department of Basic Medical Sciences and the Weldon School of Biomedical Engineering, are part of a team of researchers that has created microelectromechanical resonators, or small vibrating sensors, that can detect these biomarkers using just a drop or two of blood. The plate-style resonant sensors allows sensitive, inexpensive detection of biomarkers that can signify disease, illness or trauma.. The goal here is to find the disease so early that you can treat it ...
Multiple sclerosis (MS) is an autoimmune demyelinating disorder of the central nervous system. Only a few biomarkers are available in MS clinical practice, such as cerebrospinal fluid oligoclonal band
BLOOD MARKER FOR RENAL CANCER - The present invention provides a blood marker for renal cancer, more specifically, a blood marker that can be practically used for clinical diagnosis of renal cancer. The present invention also provides a blood marker that can be practically used for follow-up after treatment such as surgery and during treatment such as medication for renal cancer. A blood marker for renal cancer selected from the group consisting of Galectin-1, Galectin-3, and α-enolase. Galectin-1 and/or Galectin-3 as a blood marker for renal cancer for use in an examination performed before diagnostic imaging. α-Enolase as a blood marker for renal cancer for use in monitoring during and/or after treatment for renal cancer ...
Even though the post-operative outcome varies greatly among patients with nodal positive colon cancer (UICC stage III), personalized prediction of systemic disease recurrence is currently insufficient. We investigated in a retrospective setting whether genetic and immunological biomarkers can be applied for stratification of distant metastasis occurrence risk. Eighty four patients with complete resection (R0) of stage III colon cancer from two clinical centres were analysed for genetic biomarkers: microsatellite instability, oncogenic mutations in KRAS exon2 and BRAF exon15, expression of osteopontin and the metastasis-associated genes SASH1 and MACC1. Tumor-infiltrating CD3 and CD8 positive T-cells were quantified by immunocytochemistry. Results were correlated with outcome and response to 5-FU based adjuvant chemotherapy, using Coxs proportional hazard models and integrative two-step cluster analysis. Distant metastasis risk was significantly correlated with oncogenic KRAS mutations (p = 0.015),
We previously showed that IDH1 mRNA and protein expression are elevated in tumor tissues compared with matched normal tissues (10). This suggests that IDH1 plasma level might be a useful biomarker for the diagnosis of patients with NSCLCs. We have 2 hypotheses for the elevated levels of IDH1 in blood, which are currently being investigated: IDH1 may be secreted extracellularly under exceptional circumstances or released into the blood by cell damage and cell death. In addition, plasma IDH1 levels were positively correlated with T stage in patients with NSCLCs, strongly suggesting that IDH1 might be a useful diagnostic biomarker.. In this report, we showed that the plasma IDH1 level was significantly elevated in 976 patients with NSCLCs compared with 479 healthy controls. We also tested 3 existing clinical biomarkers, CEA, CA125, and Cyfra21-1, in all the samples and compared their diagnostic efficacy to that of IDH1. Then, we established the AUCs for biomarker panels and determined the ...
We are excited to present the first of our 2021 webinar series. Dr Robert Mohney, Head of Clinical and Translational Science at Owlstone Medical, will be presenting Discovery and Clinical Investigation of Non-Invasive Biomarkers using Breath Biopsy® at 1pm EST / 10am PST on January 27th.. Dr Mohney will introduce a series of case studies, showcasing the applications of breath analysis and the potential for its use in clinical practice. This webinar is for anyone interested in learning about Breath Biopsy and its capabilities for early detection of disease and precision medicine. The presentation will be followed by a live Q&A session.. Watch On Demand. Contact us. ...
IBBL recently supported the Department of Oncology (DONC) of the Luxembourg Institute of Health in advancing lung cancer diagnostics.
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
Background: The relationship between age-related frailty and the underlying processes that drive changes in health is currently unclear. Considered individually, most blood biomarkers show only weak relationships with frailty and ageing. Here, we examined whether a biomarker-based frailty index (FI-B) allowed examination of their collective effect in predicting mortality compared with individual biomarkers, a clinical deficits frailty index (FI-CD), and the Fried frailty phenotype.Methods: We analyzed baseline data and up to 7-year mortality in the Newcastle 85+ Study (n = 845; mean age 85.5). The FI-B combined 40 biomarkers of cellular ageing, inflammation, haematology, and immunosenescence. The Kaplan-Meier estimator was used to stratify participants into FI-B risk strata. Stability of the risk estimates for the FI-B was assessed using iterative, random subsampling of the 40 FI-B items. Predictive validity was tested using Cox proportional hazards analysis and discriminative ability by the ...
Plasma microRNAs (miRNAs) are promising non-invasive biomarkers for colorectal cancer (CRC) prognosis. However, the published studies to date have yielded conflicting and inconsistent results for specific plasma miRNAs.. We have conducted a study using robust assays to assess a panel of nine miRNAs for CRC prognosis and early detection of recurrence. Plasma samples from 144 patients in a prospective CRC cohort study were collected at diagnosis, 6, 12, and 24 months after diagnosis. miRNAs were assayed by Taqman qRT-PCR to generate miRNA normalised copy numbers.. Preoperative high plasma miRNA levels were associated with increased recurrence risk for miR-200b (HR [95% CI]=2.04 [1.00, 4.16], P=0.05), miR-203 (HR=4.2 [1.48, 11.93], P=0.007), miR-29a (HR=2.61 [1.34,5.07], P=0.005), and miR-31 (HR=4.03 [1.76, 9.24], P=0.001). Both plasma miR-31 (AUC: 0.717) and miR-29a (AUC: 0.703) could discriminate recurrence from these patients without recurrence. In addition, high levels of miR-31 during ...
UNLABELLED: Obesity and associated metabolic disorders have been implicated in liver carcinogenesis; however, there are little data on the role of obesity-related biomarkers on liver cancer risk. We studied prospectively the association of inflammatory and metabolic biomarkers with risks of hepatocellular carcinoma (HCC), intrahepatic bile duct (IBD), and gallbladder and biliary tract cancers outside of the liver (GBTC) in a nested case-control study within the European Prospective Investigation into Cancer and Nutrition. Over an average of 7.7 years, 296 participants developed HCC (n=125), GBTC (n=137), or IBD (n=34). Using risk-set sampling, controls were selected in a 2:1 ratio and matched for recruitment center, age, sex, fasting status, and time of blood collection. Baseline serum concentrations of C-reactive protein (CRP), interleukin-6 (IL-6), C-peptide, total high-molecular-weight (HMW) adiponectin, leptin, fetuin-a, and glutamatdehydrogenase (GLDH) were measured, and incidence rate ratios (IRRs
A Bayesian network model was developed to integrate diverse types of data to conduct an exposure-dose-response assessment for benzene-induced acute myeloid leukemia (AML). The network approach was used to evaluate and compare individual biomarkers and quantitatively link the biomarkers along the exposure-disease continuum. The network was used to perform the biomarker-based dose-response analysis,
Title : Cardiovascular Clinical Trialists (CVCT) Forum - Paris 2012 : Journal editors viewpointsSpeaker: John JARCHO, Boston, USA, NEJMDiscussant: Rita REDBERG, San Francisco, USA, Arch Intern MedAbstract : Biomarker studies have proliferated widely, but the actual impact of most such studies on the practice of medicine is modest. One major underlying problem is a lack of clarity (on the part of both investigators and readers) about what biomarker data can be expected to contribute. In many cases, studies merely demonstrate a statistical association between a putative biomarker and a clinical correlate, but the implications of this association are not made explicit. In some cases, there is an expectation that a new biomarker, if found to correlate with disease, will reveal previously unsuspected or unproven aspects of disease pathogenesis; but scientific discovery is achieved by an association study alone. Novel biomarkers may occasionally improve diagnosis, as with high-sensitivity troponin assays, or
Humans have had control over fire for at least the last 1 million years (Berna and others, 2012, PNAS, 109, 20, E1215-E1220). This control is relative, however, as human ignitions can accidentally cause wildfires such as the 2016 Nederland, Colorado fire that started from an improperly doused campfire. In areas that are not naturally fire-prone, biomass burning residue in lake cores is sometimes used as an indicator of the presence of humans in an area. New techniques using specific biomarkers can determine if and when humans lived in a region, and if the presence of humans coincides with increased fire activity.. However, specific biomarkers can help determine the timing and interpretation of climatic and anthropogenic events. For example, changing climate conditions coupled with deforestation, enforced agriculture and/or pastoralism, and the introduction of European diseases may have influenced the ability of residents to continue living in the mountains of northern New Mexico. Fecal sterols ...
SIGNIFICANCE. Oxidative stress is considered to be an important component of various diseases. A vast number of methods have been developed and used in virtually all diseases to measure the extent and nature of oxidative stress, ranging from oxidation of DNA to proteins, lipids, and free amino acids. Recent Advances: An increased understanding of the biology behind diseases and redox biology has led to more specific and sensitive tools to measure oxidative stress markers, which are very diverse and sometimes very low in abundance.. CRITICAL ISSUES. The literature is very heterogeneous. It is often difficult to draw general conclusions on the significance of oxidative stress biomarkers, as only in a limited proportion of diseases have a range of different biomarkers been used, and different biomarkers have been used to study different diseases. In addition, biomarkers are often measured using nonspecific methods, while specific methodologies are often too sophisticated or laborious for routine ...
The analysis of human serum for different biomarkers holds promise for the early detection, diagnosis and treatment of a variety of diseases, such as cystic fibrosis, arthritis and multiple types of cancer. Serum biomarkers can be used as indicators of the state of disease progression or simply as indicators for the functioning of normal biological processes within the human body. Ultimately, these biomarkers can monitor the progress of therapy [1,2]. For routine analysis of only one or a few analytes, the enzyme-linked immunosorbent assay (ELISA) remains the workhorse method for protein analysis in basic research and clinical diagnostics. Owing to increasing efforts in recent years in biomarker discovery [3,4], many high-quality antibodies with high specificity and affinity are becoming available to these markers and to entire proteomes [5-8] for basic research, early detection of cancer and other medical diagnostics [9,10].. Mass spectrometry has emerged as a powerful tool for comprehensive ...
We performed a validation study of 17 preselected plasma protein markers with reported high evidence for the prediction of eGFR decline in patients with type 2 diabetes and incident or early-stage CKD. We showed in an univariable analysis that 9 of the markers had significantly different concentration levels between patients with stable eGFR and fast progression of eGFR decline and that 14 biomarkers significantly contributed to the prediction of eGFR levels. However, most of the predictive ability was attributable to the association with baseline GFR. In the multivariable analysis of eGFR decline over time, only five markers (KIM1, FGF23, NTproBNP, HGF, and MMP1) remained significant but exhibited only a modest predictive power on top of clinical covariates. Furthermore, if the longitudinal analysis was adjusted for baseline eGFR, none of the biomarkers were able to contribute a relevant portion of explained variability, suggesting that baseline eGFR is the key variable in prediction of renal ...
About Cardiac Biomarker. A cardiac biomarker is defined as a measurable substance that is an indicator used in the diagnosis and risk stratification related to cardiovascular disease (CVDs) such as acute coronary syndrome (ACS), coronary syndromes, myocardial infarction (MI), and heart failure. Some of the cardiac biomarkers include creatine kinase (CK) MB, troponins (cTnI and cTnT), BNP, NT-proBNP, GFAP, H-FABP, LDH isoenzymes, D-dimer, IMA, MPO, myoglobin, and sCD40L. Cardiac biomarker assays help medical professionals to understand and differentiate between myocardial infarction and angina, which assists in the diagnosis and treatment of CVDs. The abnormal concentration of these biomarkers will act as significant predictors of an adverse cardiovascular condition. Cardiac point of care (POC) testing offers reliable measurement of cardiac biomarkers and provides results within one hour of testing. In addition to saving time, some cardiac POC tests have been modified to improve the accuracy of ...
Using a prospective cohort study of 2,910 Danish women with invasive breast cancer, we have demonstrated that elevated CRP levels at the time of diagnosis of breast cancer were associated with reduced overall and disease-free survival and with increased risk of death from breast cancer. These are novel observations.. Mechanistically, three components might explain the observed association between elevated CRP levels and poor breast cancer prognosis. First, tumour cell behavior: plasma CRP levels may reflect the aggressiveness of the tumour, that is, plasma CRP levels might sum up some prognostic information of well-known tumour characteristics, such as tumour stage and grade. In the present study, elevated CRP levels were indeed associated with larger tumour size, presence of distant metastases, and lower tumour grade (although CRP was not linearly associated with tumour grade), and these prognostic factors were associated with poor prognosis. Second, adjacent inflammation: plasma CRP levels ...
Cancer-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin and immunoglobulins. Moreover, biomarkers in the blood may be subjected to degradation during transportation and storage. Such degradation is a significant source of bias for cancer biomarker measurement and discovery. We have created N-isopropylacrylamide porous sieving core shell smart nanoparticles containing an internal affinity bait to perform three independent functions within minutes, in one step, in solution (serum or plasma): a) molecular size sieving with complete separation from high abundance residence proteins such as albumin and immunoglobulin, b) affinity capture of all solution phase target molecules, and c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis. In our preliminary studies we have manufactured large quantities of uniform porous nanoparticles ...
Inflammatory factors have been associated with increased risk for several cancers; several reviews over the past 10 years describe such associations (1-4). However, past epidemiologic investigations of inflammation and cancer risk have typically been small, utilized varied analysis platforms, and included a narrow range of analytes. For instance, epidemiologic studies of non-Hodgkin lymphoma (5-10), colorectal (11-13), gastric (14, 15), endometrial (16), ovarian (17-21), and lung cancer (22-24) examined a select number of inflammatory markers in various cohorts. Markers evaluated, however, have varied between studies, making interpretation of results difficult. Consequently, the precise molecular markers and pathways involved in carcinogenesis remain unclear.. Multiplex technologies have now been developed to measure larger numbers of inflammatory protein markers using small volumes of serum, plasma, and other specimens (25-31). This has created an opportunity for systematic, large-scale ...
BACKGROUND: There is limited information regarding the role of biomarker levels at predicting mortality in patients with the coronavirus disease (COVID-19) pandemic. The purpose of this study is to determine the differences in serum biomarker levels in adults with COVID-19 who survived hospitalization from those who did not. METHODS: A comprehensive search was completed on PubMed, EMBASE and Cochrane libraries to identify studies of interest. Endpoints of interest were blood counts, hepatic function test, acute phase reactants, cytokines and cardiac biomarkers. RESULTS: A total of 10 studies with 1584 patients were included in the pooled analyses. Biomarkers that were noted to be significantly higher in those who died from coronavirus disease included: white blood cell count, neutrophil count, C-reactive protein, high sensitivity C-reactive protein, procalcitonin, ferritin, D-dimer, interleukin-6, lactate dehydrogenase, creatine kinase, prothrombin time, aspartate aminotransferase, alanine
Evans, M.D., Cooke, M.S., Olinski, R., Gackowski, D., Rozalski, R., Siomek, A., Loft, S., Rossner, Jr. P., Sram, R., Henriksen, T., Poulsen, H.E., Weimann, A., Barbieri, A., Sabatini, L., Violante, F., Kino, S., Ochi, T., Sakai, K., Takeuchi, M., Kasai, H., Meerman, J.H.N., Halliwell, B., Jenner, A.M., Wang, H., Cerda, C., Saez, G., Haghdoost, S., Svoboda, P., Hu, C.-W., Chao, M.-R., Peng, K.-Y., Shih, W.-C., Wu, K.-Y., Orhan, H., Istanbullu, N.S., Mistry, V., Farmer, P.B., Sandhu, J., Singh, R., Cortez, C., Su, Y., Santella, R.M., Lambert, P., Smith, R. (2010). Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2′- deoxyguanosine as a noninvasive biomarker of oxidative stress. FASEB Journal 24 (4) : 1249-1260. [email protected] Repository. ...
Results After adjustment for covariates, statistically significant linear associations with walking duration were observed for WBC, hsTnT, Cr and CysC. CRP quartiles 1 and 2 showed no significant difference followed by a significant inverse dose-response relationship. A similar pattern, but less pronounced, was seen for N-terminal pro-brain natriuretic peptide. Mean differences between the first two quartiles of CRP and its fourth quartile were 17 min. Between categories 1 (more beneficial) and 4 of WBC, hsTnT, Cr and CysC the differences were 15, 12, 23 and 20 min, respectively.. ...
Purpose: Circulating hormone concentrations are associated with breast cancer risk, with well-established associations for postmenopausal women. Biomarkers may represent minimally invasive measures to improve risk prediction models.. Experimental Design: We evaluated improvements in discrimination gained by adding serum biomarker concentrations to risk estimates derived from risk prediction models developed by Gail and colleagues and Pfeiffer and colleagues using a nested case-control study within the EPIC cohort, including 1,217 breast cancer cases and 1,976 matched controls. Participants were pre- or postmenopausal at blood collection. Circulating sex steroids, prolactin, insulin-like growth factor (IGF) I, IGF-binding protein 3, and sex hormone-binding globulin (SHBG) were evaluated using backward elimination separately in women pre- and postmenopausal at blood collection. Improvement in discrimination was evaluated as the change in concordance statistic (C-statistic) from a modified Gail or ...
Provides device manufacturers and FDA staff with updated recommendations concerning 510(k) submissions for various types of assays for C Reactive Protein (CRP).
Colorectal cancer (CRC) remains a major public health problem with advanced and recurrent disease being a management challenge due to the lack of efficacy of currently available monitoring tools. Carcinoembryonic antigen is used as a marker for recurrent disease but has limited sensitivity. Early sensitive markers of disease severity and recurrence are required. The aim of this thesis was to identify potential metabolic biomarkers of CRC patients using Metabonomics based on proton nuclear magnetic resonance (1H NMR) spectroscopy. The breadth of 1H NMR metabonomics exploring different aspects of CRC pathogenesis was investigated. A global approach profiling a large number of metabolites was undertaken in cell line and chemoprevention studies with subsequent targeted profiling experiments using human polypoid tissue samples. The studies primarily exhibited changes in choline and lipids to be associated with CRC. Although these metabolites are well reported, this thesis presents contemporary ...
We found separated clustering in multivariate analysis (OPLS-DA) between AMI group and control group, and database search was done to find out proper metabolites to distinguish acute MI disease. As a result, several lipids and amino acid were identified as biomarker candidates. Several biomarkers such as LysoPC(18:2), LysoPC(18:1), MG(18:0(9Z)/0:0/0:0) were shown similar intensity change in each CVD disease groups, and this finding might help to find sensitive CVD diagnostic marker ...
hree renal biomarkers, acute kidney injury (AKI), albuminuria, and low estimated glomerular filtration rate (eGFR), considered separately or together, can predict adverse outcomes in diabetes
Past vegetation assemblages, preserved in peat layers, are one of the key proxies when reconstructing historical peatland dynamics. Northern peatlands can be divided into two main types: fens and bogs. Compared with bog peat, the fen peat is usually more decomposed because of different eco-hydrological conditions and effective humification processes. A high level of decomposition hampers reliable identification of plant remains and constrains palaeoecological approaches. Biogeochemical studies on bog plants and bog peat have shown that plant group-specific biomarkers can be applied to identify fossil plants or plant groups from peat, given the identification of plant group-specific markers in living fen plants. In this study, we applied plant macrofossil, biomarkers and multivariate statistical analyses to two mid-boreal peat sequences to investigate whether biomarkers can be applied to distinguish fen and bog environments and whether plant-specific biomarkers can be identified from fen peat. ...
Molecular markers may be non-biological. Non-biological markers are often used in environmental studies. In genetics, a ... "First Generation Markers", "Second Generation Markers", and "New Generation Markers". These types of markers may also identify ... Marker assisted breeding. Biochemical markers are generally the protein marker. These are based on the change in the sequence ... These markers are type-i markers. Advantages: Co-dominant markers. Less price. Disadvantages: Require prior information. Low ...
Rousseau, Jean-Charles; Delmas, Pierre D (June 2007). "Biological markers in osteoarthritis". Nature Clinical Practice ... Part 1: Markers of collagen degradation and synthesis". The Veterinary Journal. 185 (1): 36-42. doi:10.1016/j.tvjl.2010.04.011 ...
International Journal of Biological Markers. 17 (1): 5-23. doi:10.1177/172460080201700102. PMID 11936587. Lacroix, M; Leclercq ... detection and markers of disseminated breast cancer cells" Free access to "Disseminated tumor cells: detection, markers and ... Lacroix, M; Zammatteo N; Remacle J; Leclercq G. (2002). "A low-density DNA microarray for analysis of markers in breast cancer ... Lacroix., M (2006). "Significance, detection and markers of disseminated breast cancer cells". Endocrine-Related Cancer. 13 (4 ...
VOLKMAN, J. K. (1986). "Acyclic isoprenoids as biological markers". Biological Markers in the Sedimentary Record.: 1817-1828. ... Baker, E.W.; Louda, J.W. (1986). "Porphyrins in the geological record". In Johns, R.B. (ed.). Biological Markers in the ... Phytene is also found as the functional group phytyl in many organic molecules of biological importance such as chlorophyll, ... an aromatic compound believed to be markers of salinity. Therefore, this decrease in Pr/Ph should indicate an increase in ...
The term "biological marker" was introduced in 1950s. In 1987, biological markers were defined as "indicators signaling events ... "Biological markers in environmental health research. Committee on Biological Markers of the National Research Council". ... In biomedical contexts, a biomarker, or biological marker, is a measurable indicator of some biological state or condition. ... Endophenotype Imaging biomarker Marker gene Molecular marker Saliva testing Sponge biomarkers Tumor marker Hirsch MS, Watkins J ...
The International Journal of Biological Markers. 15 (2): 139-46. doi:10.1177/172460080001500203. PMID 10883887. S2CID 25940455 ... Ekman P, Lewenhaupt A (1991). "Serum tumour markers in human prostatic carcinoma. The value of a marker panel for prognostic ... As this tumor marker reacts to the activity of the tumor rather than to the tumor mass it gives a very early indication of the ... Xiang Y, Zeng H, Liu X, Zhou H, Luo L, Duan C, Luo X, Yan H (2013). "Thymidine kinase 1 as a diagnostic tumor marker is of ...
A single center experience". The International Journal of Biological Markers. 33 (1): 55-61. doi:10.5301/ijbm.5000305. ISSN ... Since then, Pro-GRP has been used as a tumor marker for patients with small cell lung cancer (SCLC) in limited and extended ... "Pro-gastrin-releasing peptide (PROGRP) as a specific tumor marker in patients with small cell lung carcinoma (SCLC)". Lung ... THE JOURNAL OF BIOLOGICAL CHEMISTRY. You, Benoit; Mercier, Frédéric; Assenat, Eric; Langlois-Jacques, Carole; Glehen, Olivier; ...
"Urinary Cell-Free DNA as a Potential Tumor Marker for Bladder Cancer". The International Journal of Biological Markers. 22 (4 ... For ucfDNA, cancer markers can be generally divided into two groups: urological cancer marker and non-urological cancer marker ... Many tumour markers in ucfDNA have been researched to monitor cancer progression, therapeutic response, and prognosis. Cancer ... Different levels and genetic markers in ucfDNA can be the indicators of the types and stages of cancer. The level of variants ...
The International Journal of Biological Markers. 22 (2): 114-23. doi:10.1177/172460080702200205. PMID 17549667. S2CID 208043955 ... Research in Biological Diversity. 74 (6): 313-21. doi:10.1111/j.1432-0436.2006.00074.x. PMID 16831200. Park ET, Oh HK, Gum JR, ... The Journal of Biological Chemistry. 277 (9): 7021-8. doi:10.1074/jbc.M108417200. PMID 11748221. S2CID 39560486. Brown NL, ...
243-. ISBN 978-3-540-32786-8. van Venrooij, W. J.; Maini, Ravinder N. (6 December 2012). Manual of Biological Markers of ... The size marker is often pipetted into the first or last pocket of a gel. For separation, the denatured samples are loaded onto ... An overloading of the gel with a soluble protein creates a semicircular band of this protein (e. g. in the marker lane of the ... A low contrast (as in the marker lane of the image) between bands within a lane indicates either the presence of many proteins ...
The International Journal of Biological Markers. 25 (3): 136-140. doi:10.1177/172460081002500303. PMID 20677166. S2CID ... The Journal of Biological Chemistry. 279 (7): 5708-5715. doi:10.1074/jbc.M307608200. PMID 14627710. van der Weyden L, Rust AG, ... The Journal of Biological Chemistry. 277 (42): 40043-40054. doi:10.1074/jbc.M205494200. PMID 12171923. Kawaida R, Ohtsuka T, ... The Journal of Biological Chemistry. 289 (10): 6775-6790. doi:10.1074/jbc.M113.499210. PMC 3945339. PMID 24394418. Ji H, ...
Manual of Biological Markers of Disease. Springer Netherlands. pp. 25-36. doi:10.1007/978-94-011-1670-1_3. ISBN 978-94-011-1670 ...
The International Journal of Biological Markers. 15 (2): 139-46. doi:10.1177/172460080001500203. PMID 10883887. S2CID 25940455 ... tumor markers). Such tumor markers are, for instance, CEA (carcinoembryonic antigen) and AFP (alpha fetoprotein). The genes for ... Wu J, Mao Y, He L, Wang N, Wu C, He Q, Skog S (2000). "A new cell proliferating marker: cytosolic thymidine kinase as compared ... A re-emergence of the disease either by accident or as a result of biological warfare would meet an unprotected population and ...
The International Journal of Biological Markers. 13 (4): 179-87. doi:10.1177/172460089801300402. PMID 10228898. S2CID 46589946 ... Mucin-16 (MUC-16) also known as Ovarian cancer-related tumor marker CA125 is a protein that in humans is encoded by the MUC16 ... MUC-16 has found application as a tumor marker or biomarker that may be elevated in the blood of some patients with specific ... The Journal of Biological Chemistry. 279 (10): 9190-8. doi:10.1074/jbc.M312372200. PMID 14676194. Gubbels JA, Belisle J, Onda M ...
Are genes good markers of biological traits? 175-193. In Biological Surveys. National Research Council Committee on Advances in ... Collecting and Utilizing Biological Indicators and Genetic Information in Social Science Surveys. Weinstein, M., Vaupel, J. W. ...
Mackenzie, Andrew Stewart (1981). Applications of biological marker compounds to subsurface geological processes (PhD thesis). ... Mackenzie, Andrew Stewart (1984). Applications of Biological Markers in Petroleum Geochemistry. London: Academic Press. ISBN ...
... a biological database of orthologous mammalian markers. The rbp3 evolutionary marker can be found, together with the ... The rbp3 gene is commonly used in animals as a nuclear DNA phylogenetic marker. The exon 1 has first been used in a pioneer ... Gene structure and primary structure". The Journal of Biological Chemistry. 264 (14): 8200-6. doi:10.1016/S0021-9258(18)83169-6 ... The Journal of Biological Chemistry. 271 (34): 20507-15. doi:10.1074/jbc.271.34.20507. PMID 8702792. Shaw NS, Noy N (Feb 2001 ...
Taioli, Emanuela; Bonassi, Stefano (2003-01-01). "Pooled analysis of epidemiological studies involving biological markers". ...
Rechenberg DK, Galicia JC, Peters OA (2016-11-29). "Biological Markers for Pulpal Inflammation: A Systematic Review". PLOS ONE ...
"Biological Markers for Pulpal Inflammation: A Systematic Review". PLOS ONE. 11 (11): e0167289. Bibcode:2016PLoSO..1167289R. doi ... Michael A Henry, et al, Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show ... Other peptides include cGRP, galanin, somatostatin, and neurokinin A-B. The biological effects of SP are expressed by the ...
... there are still no biological markers for body image disturbance. Numerous psychometric instruments to measure the cognitive, ... Biological Psychiatry. 58 (12): 990-997. doi:10.1016/j.biopsych.2005.06.001. ISSN 0006-3223. PMID 16084858. S2CID 15508818. ... "Visual mapping of body image disturbance in anorexia nervosa reveals objective markers of illness severity". Scientific Reports ... The World Journal of Biological Psychiatry. 12 (6): 462-472. doi:10.3109/15622975.2010.551666. ISSN 1562-2975. PMID 21554026. ...
There is no availability of genetic testing or biological markers. The patients presented with a history of febrile illness ...
Therefore, more studies using experimental models are needed in order to confirm the validity of sAPP as a biological marker ... June 2010). "Biological markers of amyloid beta-related mechanisms in Alzheimer's disease". Experimental Neurology. 223 (2): ... Recent studies primarily focus on use of an autoantibody, not only for biological markers but for future treatment. However, ... Although the biological function of APP are not known, it has been hypothesized that APP may play a role during ...
"Minichromosome maintenance proteins as biological markers of dysplasia and malignancy". Clin. Cancer Res. 5 (8): 2121-32. PMID ...
Markers of biological effect provide an estimation of exposure based on cellular activities related to the mechanism of action ... While measuring biomarkers or markers of biological effects may provide more accurate estimates of exposure, collecting these ... Two broad approaches include measuring biomarkers and markers of biological effect. The former involves taking direct ... Biological pesticides based on entomopathogenic fungi, bacteria and viruses causing disease in the pest species can also be ...
Main topics are heart failure, chronic disease, cachexia and biological markers. Stefan D. Anker publications indexed by Google ... "Mid-region pro-hormone markers for diagnosis and prognosis in acute dyspnea: results from the BACH (Biomarkers in Acute Heart ...
Summons, Roger E.; Powell, Trevor G. (1986). "Chlorobiaceae in Palaeozoic seas revealed by biological markers, isotopes and ... Combined evidence from biological markers, isotopes and geology 1987 - 1998 - Fellow, Royal Australian Chemical Institute 1998 ... Analogues for biological oases during the Cryogenian Organic matter preserved in 3-billion-year-old mudstones at Gale crater ... Analogues for biological oases during the Cryogenian". Geobiology. 16 (4): 369-377. doi:10.1111/gbi.12280. ISSN 1472-4677. PMID ...
"The biological characterization of neuroendocrine tumors: The role of neuroendocrine markers". Journal of Endocrinological ... NETs show tissue immunoreactivity for markers of neuroendocrine differentiation (pan-neuroendocrine tissue markers) and may ... several reviews provide assistance in understanding these markers. Widely used neuroendocrine tissue markers are various ... The nuclear neuroendocrine marker insulinoma-associated protein-1 (INSM1) has proven to be sensitive as well as highly specific ...
Rodriguez T (2015). "Identifying significant biological markers in Klotho gene variants across wide ranging taxonomy". Journal ... Olejnik A, Franczak A, Krzywonos-Zawadzka A, Kałużna-Oleksy M, Bil-Lula I (2018). "The Biological Role of Klotho Protein in the ... The Journal of Biological Chemistry. 289 (52): 35849-57. doi:10.1074/jbc.M114.616649. PMC 4276853. PMID 25378396. Saghiv MS, ...
"Specific acyclic isoprenoids as biological markers of methanogenic bacteria in marine sediments". Nature. 290 (5808): 693-696. ... Lycopane has not yet been directly isolated in any biological organism, so its linkage to methanogenic archaea is conjecture. ... Diagenesis, broadly referring to physical and chemical changes that occur while biological material is undergoing fossilization ... as diagenesis occurs during biological fossilization. Freeman, Katherine H.; Hayes, J. M.; Trendel, Jean-Michel; Albrecht, ...
In Road Block Buster the hero must "jump[] around [] doing tricks to soldiers ... [attempt] to get over any barrier or road ... biological and mental-health factors." This conclusion supports Surgeon General Satcher's 2001 study (supra). Despite this ...
Wu J, Li L, Wu S, Xu B (August 2020). "CMTM family proteins 1-8: roles in cancer biological processes and potential clinical ... autoimmune diseases and can serve as clinical markers of disease severity and/or as therapeutic targets for controlling the ... International Journal of Biological Sciences. 15 (12): 2576-2583. doi:10.7150/ijbs.33733. PMC 6854381. PMID 31754330. Cai B, ... to confirm and expand these relationships and determine if the expression of CMTM5 isoforms can be used as tumor markers for ...
The Journal of Biological Chemistry. 251 (7): 2005-14. doi:10.1016/S0021-9258(17)33647-5. PMID 1270419. Sára, M; Sleytr, U. B ... "Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker". Molecular ...
Suzuki surpassed his own biological heir and named Hirotaka Ono as his successor, because he felt that his biological son was ... This does not mean that there were no vertical jumps in the social stratum by less wealthy individuals, but it was ... If the adoptee decides to go back to their biological family, goes missing or deserts their adopted family, the adoption can ... In the past, merchant families in Western Japan would adopt an heir if the biological predecessor was incapable of taking over ...
Mulder LC, Muesing MA (Sep 2000). "Degradation of HIV-1 integrase by the N-end rule pathway". The Journal of Biological ... More recently, more effort has been made to consider the proteasome for the development of novel diagnostic markers and ... The CP and RPs pertain distinct structural characteristics and biological functions. In brief, 20S sub complex presents three ... To meet such complicated demands in biological process via spatial and temporal proteolysis, protein substrates have to be ...
They run with rabbit-like jumps.[citation needed] In the groin of each leg is an inguinal gland used for scent marking; this ... Biological Sciences. 265 (1398): 793-801. doi:10.1098/rspb.1998.0362. PMC 1689037. PMID 9628037. Christian Pitra; Joerns Fickel ...
At 25 he became a jet fighter pilot and later became a pilot and parachute instructor with over 72 jumps by the time he began ... The four conducted biological experiments on themselves during their stay. Bykovsky and Jähn undocked from the station in the ...
Asexuality as a sexual identity, rather than as a biological entity, became more widely discussed in the media in the beginning ... Alix freezes time through the adrenaline rush of BASE jumping. In 2019, Bo came out to her friend, Lola, in an issue of the ...
They encountered Malcolm Merlyn (who will join to them 8 years later) and Darius when searching for Ashkiri marker (in fact, ... She is also the biological mother of Cassandra Cain. David Cain - First appearing in Batman #567 (July 1999), he is the ... biological father of Cassandra Cain and the adoptive father of Mad Dog III. Cassandra Cain (brainwashed at the time) - First ...
That is to say, not only because of the biological, critical sense, but because of its poetic and abyssal nature. The song ... For instance, the poem begins: "Tree, leaf, jump, light, approximation, furniture, wool, taste, foot, tea, sea, gas, look" ( ...
analyzed king cherry and Yoshino cherry with inter-simple sequence repeat (ISSR) markers and sequence analysis of two ... Korean Red List of Threatened Species (PDF) (Second ed.). National Institute of Biological Resources. 2014. p. 156. Prunus × ... reported that the Sequence-level comparison of Prunus Conserved Orthologous Gene Set (Prunus COS) markers suggested that king ... using four different Rosaceae Conserved Orthologous Set (RosCOS) markers, provided evidence that king cherry originated from ...
Sidney LE, Branch MJ, Dunphy SE, Dua HS, Hopkinson A (June 2014). "Concise review: evidence for CD34 as a common marker for ... The Journal of Biological Chemistry. 265 (19): 11056-11061. doi:10.1016/S0021-9258(19)38556-4. PMID 1694174. Sutherland DR, ... This is generally a useful marker for cell dosing although there is some evidence that the CD34+ quantification may not be ... Furness SG, McNagny K (2006). "Beyond mere markers: functions for CD34 family of sialomucins in hematopoiesis". Immunologic ...
A one-shot was first published in Shueisha's Jump Next! in April 2012. The manga began its serialization in Weekly Shōnen Jump ... whereas Mana and Azami discover that Asahi is Azami's biological son and Erina's half-brother, leading the former to request ... Ressler, Karen (July 16, 2018). "One Piece/Food Wars! Spinoff Manga About Sanji to Run in English Shonen Jump". Anime News ... It was serialized in Shueisha's Weekly Shōnen Jump from November 2012 to June 2019. Its chapters were compiled in 36 tankōbon ...
A marker in the park was dedicated to recognize the point where the group crossed the Santa Ana River. To commemorate De Anza's ... A U. S. Department of Agriculture scientist helped growers to harness nature's biological wrath during the "decay crisis" of ...
Most aspects of gameplay-such as player guidance assists like map markers and enemy locations, NPC behavior like radio callouts ... a malaria super-strain used as a biological weapon. Santiago gathers evidence for Miller, whose plan to assault the lab ...
The DNA oxidation product 8-oxoguanine (8-oxoG) is a well-established marker of oxidative DNA damage. In persons with ... Taylor RW (February 2005). "Gene therapy for the treatment of mitochondrial DNA disorders". Expert Opinion on Biological ... Butler, John M. (2005). Forensic DNA typing : biology, technology, and genetics of STR markers (2nd ed.). Amsterdam: Elsevier ... Since animal mtDNA evolves faster than nuclear genetic markers, it represents a mainstay of phylogenetics and evolutionary ...
Through his published work, he is a proponent of the theory that male infertility is an early marker for other diseases that ... Belknap Prize in the Biological Sciences, and co-authored several scientific publications from work in the laboratory of Dr. ... the toxic effect of medications such as the antioxidant selenium and the anti-inflammatory drug class called biological ...
Sex can also be guessed using visual markers, but it is typically not nearly as accurate. Male ackie monitors are generally ... Biological Journal of the Linnean Society. 35 (4): 379-407. doi:10.1111/j.1095-8312.1988.tb00477.x. ISSN 0024-4066. "Ackie ...
After Emma commits suicide by jumping from a viaduct, the ghosts of both James and Finn appear to welcome Emma to the afterlife ... Moira and James are seen to be arguing in the background and it is later revealed that James is Adam's biological father. In ...
Biological Psychiatry. 74 (12): 872-8. doi:10.1016/j.biopsych.2013.05.008. PMID 23806573. S2CID 381132. Nuttall JR, Oteiza PI ( ... Int J Biol Markers. 18 (3): 162-169. doi:10.1177/172460080301800302. PMID 14535585. Theocharis SE, Margeli AP, Klijanienko JT, ...
... s, also known as microplate readers or microplate photometers, are instruments which are used to detect biological ... Label-free instruments that use specialized microplates to measure binding events without the use of chemical markers Neves, ...
Yustian, Indra; Marker, Stefan; Supriatna, Jatna; Andayani, Noviar (2008). "Relative Population Density of Tarsius diane in Man ... Miller, GS; Hollister, N (1921). "Twenty new mammals collected by H.C.Raven in Celebes". Proceedings of the Biological Society ...
This ultimately leads Sam to deduce that they are biological siblings, and to inform Blair that Jack is her biological son. She ... In 2012, Todd jumps bail and arrives in Port Charles upon learning that Starr has been involved in a car accident that resulted ... Todd barges in on Starr and Cole in bed together for the first time, jumping to the conclusion that Cole had raped her, "and [ ... With help from Todd's scheming biological sister, Tina, David claims to be the lost Lord heir, but Todd's true parentage is ...
Frösö Zoo also had a tropical house, a biological museum and an amusement park. Outside of Östersund in the village of Orrviken ... The very first civilian parachute jump was done actually on the ice of Storsjön in February 1956. The jumpers were officers ... from the Swedish Parachute rangers regiment that also wanted to jump on their spare time. Östersund's Fallskärmsklubb is today ...
Although proteomics and cytomics allow for the early detection of disease, much of the time those detect biological markers ... Schulte PA; Lomax GP; Ward EM; Colligan MJ (August 1999). "Ethical issues in the use of genetic markers in occupational ...
Wrangham R, Conklin-Brittain N (September 2003). "Cooking as a biological trait" (PDF). Comp Biochem Physiol A. 136 (1): 35-46 ... and other El Niño markers. The Paleolithic is often held to finish at the end of the ice age (the end of the Pleistocene epoch ...
She is introduced in Metal Gear Solid as Solid Snake's sidekick/love interest and Roy Campbell's legal niece/biological ... and also climb and jump around in trees with ease. He uses two crossbows in battle, the Little Joe and the William Tell, ... the biological mother of Ocelot, and one of the main antagonists of Metal Gear Solid 3: Snake Eater. The Boss is voiced by ... though Paz was not aware of this when she jumped from the helicopter, and the explosion was actually caused by an enemy rocket ...
Bobby proposes to Marvin that he jump-start his career by shooting him in the "fleshy part of the thigh." The fee agreed is $ ... Aunt Dottie: Paulie's biological mother, who dies of natural causes. When discussing ways to increase Marvin the rapper's ...
... the biological son of Kevin who was sent to live with his father after the death of his biological mother. Jonathan Loughran as ... "Jump" by Van Halen background music on the answering machine message in Sonny's apartment "Growin' Up" by Bruce Springsteen " ... A note states that his mother is no longer able to care for him and that Kevin is his biological father. Sonny contacts Kevin, ... Jon Stewart as Kevin Gerrity, Sonny's roommate and Julian's biological father, which he realizes was the result of a one-night- ...
Christian tells her that he has never done anything like skydiving before and is not sure he will get to jump today, but Rachel ... The character's biological father Matthew Montgomery (James Sweeny) was introduced in November 2021. After 18 months in the ... but he also gets to jumps into a police car and chase down a criminal. Cash's sister Felicity Newman (Jacqui Purvis) is later ...
Using a multivariate-fused approach involving two widely used imaging markers, we demonstrate both shared and distinct ... Using a multivariate fused approach involving two widely used imaging markers we demonstrate both shared and distinct ... biological traits across the psychosis spectrum. Further, our results suggest that the above traits are psychosis biomarkers ... Large-Scale Fusion of Gray Matter and Resting-State Functional MRI Reveals Common and Distinct Biological Markers across the ...
Seeking biological markers after a TBI. ... Seeking biological markers after a TBI. ...
Consensus paper of the WFSBP Task Force on Biological Markers of Dementia: the role of CSF and blood analysis in the early and ... Consensus paper of the WFSBP Task Force on Biological Markers of Dementia: the role of CSF and blood analysis in the early and ... Mild cognitive impairment (part 2): biological markers for diagnosis and prediction of dementia in Alzheimers disease ... The search and development of biological markers of AD pathogenesis. Translational research has been critical for the ...
Role of biological markers and CT severity score in predicting mortality in patients with COVID‑19: An observational ... Todor S, Bîrluțiu V, Topîrcean D and Mihăilă R: Role of biological markers and CT severity score in predicting mortality in ... Todor, S., Bîrluțiu, V., Topîrcean, D., Mihăilă, R.Role of biological markers and CT severity score in predicting mortality in ... Todor, S., Bîrluțiu, V., Topîrcean, D., Mihăilă, R.Role of biological markers and CT severity score in predicting mortality in ...
title = "Biological markers of myocardial necrosis",. abstract = "New biological markers of myocardial injury have improved the ... Biological markers of myocardial necrosis. In: Revista Espanola de Cardiologia. 2003 ; Vol. 56, No. 7. pp. 703-720. ... N2 - New biological markers of myocardial injury have improved the management of patients with acute coronary syndromes. Among ... AB - New biological markers of myocardial injury have improved the management of patients with acute coronary syndromes. Among ...
Biological markers. Over the past 10 years, great enthusiasm has been noted regarding the potential of biological markers, such ... Tularemia as a biological weapon: medical and public health management. JAMA. 2001 Jun 6. 285(21):2763-73. [QxMD MEDLINE Link] ...
Although biological markers of womens exposure to semen from vaginal intercourse have been developed as surrogates for risk of ... Detection of two biological markers of intercourse: prostate-specific antigen and Y-chromosomal DNA. ... Detection of two biological markers of intercourse: prostate-specific antigen and Y-chromosomal DNA ... Title : Detection of two biological markers of intercourse: prostate-specific antigen and Y-chromosomal DNA Personal Author(s ...
Biological markers. The most widely available biological marker for staging acute pancreatitis is the hematocrit value. ... Early studies of IL-6 as a biological marker appear promising, indicating that this may be a reliable indicator of pancreatitis ... Genetic markers. Polymorphisms in the chemokine monocyte chemotactic protein 1 (MCP-1) gene may play a role in predisposing ... CRP, a nonspecific acute-phase reactant produced by the liver in response to interleukin (IL)-6, is a useful marker at 36-48 ...
Biological inflammatory markers mediate the effect of preoperative pain-related behaviours on postoperative analgesics ... Biological inflammatory markers mediate the effect of preoperative pain-related behaviours on postoperative analgesics ...
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GASSEN, Humberto Thomazi et al. Expression of biological markers in oral squamous cell carcinomas. Stomatos [online]. 2012, vol ... Palavras-chave : Mouth neoplasms; Squamous cell carcinoma; Tolonium chloride; Biological markers. · resumo em Português · texto ... Our findings suggest an important contribution of tumor markers in the diagnosis and prognosis of these malignancies, as well ... and microscopic features of squamous cell carcinomas using immunohistochemical analysis and assessing biological markers of ...
Biological monitoring of chemical exposure in the workplace : guidelines. by World Health Organization. Office of Occupational ... DNA adducts : identification and biological significance / edited by K. Hemminki ... [et al.] by Hemminki, K , Dipple, A , ...
... ANI. 05 Oct 2022, 03:03 GMT+10 ... "Efforts have been underway for decades to identify genetic or other biological markers for PPD. ...
Biological markers included immune and endocrine serum markers, neuroimaging techniques, and electrophysiological outcomes. In ... Biological marker outcomes were used as predictors of response, to understand mechanisms of TMS, and as a surrogate of safety. ... Biological Markers in Noninvasive Brain Stimulation Trials in Major Depressive Disorder: A Systematic Review. Fidalgo TM, ... there is a lack of mechanistic studies using biological markers (BMs) as an outcome. Therefore, our aim was to review ...
CONSTANTINO, Michel et al. Behavioral economics: design of an experiment with Biological Marker 2D:4D. Rev. Psicol. Saúde [ ... 4D ratio biological marker (Fernandes et al., 2006; Dreber e Hoffman, 2007); Teixeira et al., Teixeira et al. , 2014 and Silva ... Tabak, 2015). A growing number of researches have tried to understand the nature of human behavior by adding biological factors ...
Biological/surrogate markers of survival. The beneficial effects of physical activity on cancer survival may be mediated ... A second strong surrogate or biological marker associated with breast cancer survival is insulin values. There has been ... randomised controlled trials testing the effects of physical activity on cancer survival and/or surrogate/biological markers ... a small number of randomised trials of exercise on surrogate/biological markers of survival have been published. ...
Pleural Transport Physiology: Insights from Biological Marker Measurements in Transudates Eleni Apostolidou*, 1, Irini Tsilioni ... The biological markers studied were lactate dehydrogenase (LDH), adenosine deaminase (ADA), interleukin-6 (IL-6), C-reactive ... No correlation between the molecular radius and the pleural fluid to serum ratio of the above biological markers was found. ... Keywords: Biological markers, mesothelial barrier, pleural fluid/ serum ratio, transudates, lactate dehydrogenase, tumor ...
Keywords: Biological markers; Clinical trial; Outcome assessment (health care); Reference Standards; Research design; ...
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NABIL BOUDERSA, GHANIA CHAIB , ATOUI AICHA , RADIA CHERFIA, HANANE BOUDERBANE, LEILA BOUDOUR, Assessment of biological and ... Genetic diversity in several genotypes of Algerian lentil using biochemical markers. Biodiversitas 22: 3494-2500. Studying seed ... Genetic diversity in several genotypes of Algerian lentil using biochemical markers ##plugins.themes.bootstrap3.article. ... The Idlep3 genotype is characterized by specific bands (or a specific marker), followed by the Flip90-31 genotype. So these two ...
Urinary chromium as a biological marker of environmental exposure: What are the limitations? Regul Toxicol Pharmacol 26(1 Pt 2 ...
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New model to identify biological markers in patients with COVID-19 and other illnesses Investigators who recently developed a ... why treatment responses vary widely among individuals with COVID-19 have now used the model to identify biological markers ...
Biological markers as outcome measures for Alzheimers disease interventions - real problems and future possibilities - Volume ... Biological markers as outcome measures for Alzheimers disease interventions - real problems and future possibilities. ... No single biological marker can serve all the purposes of screening, differential diagnosis and measurement of severity. ... Biological markers that reflect molecular stress, such as oxidative, ribotoxic, and nitroso stress, need to be developed, ...
Association Between Behavioral, Biological, and Genetic Markers of Cardiovascular Health and MRI Markers of Brain Aging: A ... Association Between Behavioral, Biological, and Genetic Markers of Cardiovascular Health a ... We sought to investigate the associations between behavioral, biological, and genetic markers for CVH and vascular brain aging ... unfavorable) biological CVH profiles were significantly related to slower WMH increase only in people aged 60-72 years. CVH ...
... through clinical or biological markers, in order to better select patients who could benefit from targeted therapy with ... Are there reliable clinical or biological markers predictive for subgroups of patients who may benefit from cetuximab therapy, ... Are there reliable clinical or biological predictive markers for bevacizumab therapy?. Several retrospective analyses of the ... Overall, to date, no clinical or biological markers predictive of bevacizumab activity in advanced NSCLC are reliable ...
  • The identification of individuals at risk of dementia with no detectable clinical manifestations of this syndrome parallels the attempts to determine the risk of the future occurrence of major disease events in the light of measurable underlying susceptibility and/or pathological markers at early stages of the clinical trajectory of the disease. (
  • However, these markers also have limitations, and their concentrations should always be interpreted in the light of the patient's clinical status. (
  • The objective of this study was to evaluate clinical and microscopic features of squamous cell carcinomas using immunohistochemical analysis and assessing biological markers of angiogenesis and tumor vascular activity (anti-CD31, anti-CD34, Factor VIII), cell proliferation (Ki-67), and loss of cell suppression (p53). (
  • Because the biological pathological process is observed decades years before the clinical onset of Alzheimer's disease (AD), there is a theoretical ad-vantage in using biological markers for the early diagnosis of AD. (
  • Biological markers that reflect molecular stress, such as oxidative, ribotoxic, and nitroso stress, need to be developed, particularly for measuring the clinical outcomes of interventions. (
  • A number of ongoing trials have been specifically addressed to answer them, however, the first priority should be to personalize treatment, through clinical or biological markers, in order to better select patients who could benefit from targeted therapy with decreased toxicity. (
  • Biological Markers in Fundamental and Clinical Medicine (scientific Journal) , 2 (2), 94-96. (
  • OBJECTIVES: To evaluate the diagnostic utility of serological markers for C. trachomatis in different clinical groups of STD patients. (
  • Among these markers, the most relevant are the cardiac troponins (troponin I and troponin T) because of their cardiospecificity, and myoglobin because of its combination of diagnostic sensitivity and usefulness for an early diagnosis. (
  • Our findings suggest an important contribution of tumor markers in the diagnosis and prognosis of these malignancies, as well as in treatment planning. (
  • Neuro-psychological test batteries, brain imaging and biological markers are expected to be used for screening and differential diagnosis of dementia and also for evaluation of the efficacy of early intervention. (
  • No single biological marker can serve all the purposes of screening, differential diagnosis and measurement of severity. (
  • As well as providing a better understanding of the molecular pathogenesis of AD, there is a possibility of finding a surrogate marker of AD, which might fulfill the requirement of sufficient sensitivity and specificity for AD diagnosis, as well as indicating the disease-modifying activity of interventions. (
  • To gain advanced understanding of critical issues in working with substance misuse clients concerning, diagnosis, classification and identification of biological markers of addiction. (
  • Also determination of biological markers on lumbar fluid for the diagnosis of alzheimer's. (
  • The follow-up of the cases for several years after diagnosis (biological samples and data collected by questionnaire) will provide a unique resource to assess the role of lifestyle habits and genetic factors in prognosis and second primary cancer occurrence. (
  • June 15, 2009) - A team of researchers led by Dr. Jayakrishna Ambati at the University of Kentucky has discovered a biological marker for neovascular age-related macular degeneration (AMD), the leading cause of blindness in older adults. (
  • Biological monitoring of chemical exposure in the workplace : guidelines. (
  • A growing number of researches have tried to understand the nature of human behavior by adding biological factors, especially exposure to the hormone testosterone. (
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  • The primary objective of this proposal was to develop biological markers of human exposure to woodsmoke. (
  • In this study we examine whether the mechanism of secretase activity will offer a new surrogate marker of AD. (
  • Microsatellite markers, also known as simple sequence repeats (SSR), are often used to study the genetic diversity of plants and animals. (
  • The objective of this study was to develop a set of microsatellite markers to be used to evaluate the genetic diversity and structure of E. erythropappus and other species of the Eremanthus genus. (
  • Highly polymorphic microsatellite markers can be used to identify individuals in the population. (
  • High tumor expression of STMN1 was a prognostic marker in patients with osteosarcoma for poor prognosis (P=0.016), poor response to chemotherapy (P=0.004), the presence of metastases (P=0.003), advanced Enneking surgical stage (P=0.014), and the chondroblastic osteosarcoma subtype (P=0.004). (
  • Enhanced neutrophil respiratory burst as a biological marker for manipulation forces: duration of the effect and association with substance P and tumor necrosis factor. (
  • These include genetic predisposition, biological markers, and lifestyle factors. (
  • European researchers are working to identify biological markers and risk factors associated with cardiometabolic diseases. (
  • The signal is among the latest of several such markers, including factors detectable in blood, genetic markers, and a sleep-specific brain wave, recently uncovered by the NIH team and grantee collaborators. (
  • Determining the factors that increase these markers can help limit the severity of RA. (
  • Lastly, the study noted changes in biological factors increase markers as well. (
  • ABSTRACT The value of serum tumour markers in the prognosis of patients with breast cancer is controversial. (
  • This article aims to demonstrate an experimental design in the area of behavioral economics and the inclusion of the research based cognitive bias of the 2D:4D ratio biological marker (Fernandes et al. (
  • Association Between Behavioral, Biological, and Genetic Markers of Cardiovascular Health and MRI Markers of Brain Aging: A Cohort Study. (
  • We sought to investigate the associations between behavioral, biological , and genetic markers for CVH and vascular brain aging in older adults . (
  • At baseline, global, behavioral, and biological CVH metrics were defined and scored following the Life 's Simple 7 approach and categorized into unfavorable, intermediate, and favorable profiles according to tertiles. (
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  • Thus, shared abnormalities between probands and unaffected relatives can serve as endophenotypes, which may provide biological genetic substrates for improved diagnostic classification ( 12 ), and ultimately may lead to better, more focused treatments. (
  • The serial analysis and combined use of both markers fulfill all diagnostic and prognostic requirements, and are helpful in indicating therapeutic strategies for acute coronary syndromes. (
  • IMSEAR at SEARO: Diagnostic utility of serologic markers for genital chlamydial infection in STD patients in Chennai, India. (
  • Joyee AG, Thyagarajan SP, Vikram Reddy E, Rajendran P, Venkatesan C, Ganapathy M. Diagnostic utility of serologic markers for genital chlamydial infection in STD patients in Chennai, India. (
  • Microsatellites were evaluated as genetic markers for the mitochondrial genome (mtDNA) of Phytophthora cinnamomi for population studies. (
  • Zarate, Brian Cornwell, Ph.D., and NIMH colleagues report on their brain imaging study online in the journal Biological Psychiatry . (
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  • Biological markers included immune and endocrine serum markers, neuroimaging techniques, and electrophysiological outcomes. (
  • Biological marker outcomes were used as predictors of response, to understand mechanisms of TMS, and as a surrogate of safety. (
  • Other biological outcomes include changes in markers of neuronal damage, oxidative stress, and pro- and anti-inflammatory cytokines. (
  • A background in the biological sciences is not required. (
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  • The program is designed to provide the student with a broad base in modern biological thought, to fulfill the basic requirements for admission to professional and advanced degree studies and to prepare graduates for careers in the biological sciences and related fields. (
  • The honours year provides students with the opportunity for either broadening their understanding of the biological sciences or for increased specialization. (
  • Students must apply to the Department of Biological Sciences in order to be admitted to year 4 (honours). (
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  • Compared with the survivor patients, non‑survivors had a higher prevalence of diabetes, chronic kidney disease and coronary heart disease, as well as higher values of laboratory markers such as neutrophil‑lymphocyte ratio (NLR), D‑dimer, procalcitonin, IL‑6 and C Reactive protein (CRP) and respectively high values of imaging severity scores. (
  • Early identification of markers associated with disease severity and mortality can lead to a prompter therapeutic approach. (
  • Laboratory markers and imaging severity scores could be used to stratify mortality risk in COVID‑19 patients. (
  • No correlation between the molecular radius and the pleural fluid to serum ratio of the above biological markers was found. (
  • Microsatellite loci present the highest information content among all classes of molecular markers [ 27 ]. (
  • Their duration of benefit and availability to the molecular characteristics of pathogens, such as viru- appears limited at the biological level, a constraint not seen lence, transmissibility, and survival fitness, which are with therapies for other disease conditions. (
  • The study team, formerly based at the Salk Institute for Biological Studies in La Jolla, Calif., published their current findings online March 8 in Molecular Cell. (
  • These solutions are primarily in molecular diagnostics, which allow clients to analyze biological markers. (
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  • The company's Light Treatment platform combines biological insights and genetic marker knowledge to mediate genetic expression with a one-time application of light signals. (
  • Auraptene (AUR) and naringenin (NAR) are citrus-derived phytochemicals that influence several biological mechanisms associated with cognitive decline, including neuronal damage, oxidative stress and inflammation. (
  • This study will evaluate whether an intervention with citrus peel extract standardized in levels of AUR and NAR has cognitive and biological effects in older adults with SCD, facilitating the establishment of nutrition intervention in people at risk of cognitive decline. (
  • These findings suggest that SCD may be an early-stage marker for AD and a potential target to test interventions aimed at maintaining cognitive function as long as possible. (
  • In order to find epigenetic markers for schizophrenia, the researchers used a machine learning algorithm to look for DNA methylation patterns in specific regions of the human genome called CoRSIVs. (
  • A number of previous studies have analyzed methylation profiles in blood samples in an attempt to identify epigenetic markers of schizophrenia, the researchers noted. (
  • Researchers at the Tabriz University of Medical Science found pain and sleep affect inflammatory markers in rheumatoid arthritis (RA) patients. (
  • Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. (
  • The total number of alleles was 38 and the number of alleles per polymorphic marker ranged from 2 to 18 (Table 1 ). (
  • New biological markers of myocardial injury have improved the management of patients with acute coronary syndromes. (
  • Biological markers of acute kidney injury. (
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  • Their analysis of blood samples identified epigenetic markers - part of your DNA - that differ between people with schizophrenia and those without the mental health disorder. (
  • DNA methylation is a common epigenetic marker. (
  • To figure out whether the effects of age could be reversed, they measured biological age using epigenetic clocks on several tissues. (
  • The epigenetic clocks revealed a reduced biological age for several organs in long-term treated genetically modified mice. (
  • The marker, a receptor known as CCR3, shows strong potential as a means for both the early detection of the disease and for preventive treatment. (
  • CCR3 chemokine receptor is known to be a key player in the allergic inflammation process, but Dr. Ambati's studies have now identified CCR3 as a key marker of the CNV process involved in AMD. (
  • Through a combination of microscopic simulations and fluorescence microscopy we find the lipid transport rate catalyzed by the DNA nanostructure exceeds 10 7 molecules per second, which is three orders of magnitude higher than the rate of lipid transport catalyzed by biological enzymes. (
  • The hypothesized existence of cancer stem cells (CSC) and its markers aldehyde dehydrogenase 1 (ALDH1), CD44, SOX2 and OCT4 in oral dysplastic tissues provides the potential for a more reliable assessment of malignant transformation of oral epithelial dysplasia (OED). (
  • The expression of CSC markers in OED and normal mucosa differs significantly with co-expression of all four markers located only in dysplastic tissues. (
  • Thus, the present study is intended to evaluate the immunohistochemical expression of four different CSC markers ALDH1, CD44, SOX2 and OCT4 in different grades of OED and to investigate the co-expression of these putative stem cell markers in OED. (
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  • This book will enrich students' appreciation of the nexus of biology and crime--without negating non-biological theories of crime. (
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  • The idea is to predict ahead of time biological markers in the treatment of tumors," Prof. Ciechanover said. (
  • Through the computational evaluation of markers of oxidation (plasma Mitoxantrone distributor malondialdehyde, oxidized glutathione and urinary isoprostanes) and antioxidants (decreased glutathione, tocopherol and plasma antioxidant capability) an index of global oxidative tension (OXY-SCORE) continues to be proposed6. (
  • Compared to adult cells, umbilical cord MSCs possess the highest proliferative potential (ability to replicate) and biological activity, making them one of the main candidates for use in regenerative medicine and cell therapy. (
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  • New biological research reveals how an invading virus hijacks a cell's workings by imitating a signaling marker to defeat the body's defenses. (
  • NeoClone Biotechnology International , which develops monoclonal antibodies for use in research, has landed a $750,000 federal contract to continue research into biological markers that signal ovarian cancer, according to a report in the Wisconsin State Journal . (
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  • Co-expression of all four markers was found in 15 cases of OED with none of the normal cases showing co-expression. (
  • Efforts have been underway for decades to identify genetic or other biological markers for PPD. (
  • Investigators who recently developed a mathematical model that indicated why treatment responses vary widely among individuals with COVID-19 have now used the model to identify biological markers related to these different responses. (
  • Scientists have discovered a biological marker that may help to identify which depressed patients will respond to an experimental, rapid-acting antidepressant. (
  • The aim is also to identify biological markers of outcome. (
  • Until now, no single protein marker has been able to unequivocally identify the CSCs. (
  • In addition, levels of fibrin related markers (D-dimer) are moderately or significantly elevated, suggesting coagulation activation, especially in dead patients ( 13 ). (
  • This prospective study in Yazd, Islamic Republic of Iran, assessed the value of the tumour markers carcinoembryonic antigen (CEA) and cancer antigen (CA) 15-3 in 159 patients with primary breast cancer. (
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  • Thus, a panel of putative CSC markers will help in identifying the patients with high risk for malignant transformation in OED. (
  • The aim of this study was to evaluate the physicochemical properties of the pleural mesothelial barrier and of the biological markers that facilitate or eliminate the passage of molecules through the pleura. (
  • Using blood-derived inflammation profiles measured by a multiplex array in 268 participants from the Italian component of the European Prospective Investigation into Cancer and Nutrition cohort, we evaluate the association between early life, young adulthood and later adulthood SEP with each inflammatory markers separately, or by combining them into an inflammatory score. (
  • By the means of measuring the function ability of its physiological systems, it is possible to evaluate the biological age and use it as an indicator of premature aging. (
  • Without a biological marker to confirm cases of AFM, classification of cases is challenging. (
  • Contribution of biological markers to occupational health. (
  • Favorable and intermediate (vs. unfavorable) biological CVH profiles were significantly related to slower WMH increase only in people aged 60-72 years. (
  • Fractional excretion of sodium (FENa) is a marker of renal tubular function, and is a significantly cheaper investigation [ 4 ]. (
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  • To explore potential biological embedding and the consequences of SEP experiences from early life to adulthood, we investigate how SEP indicators at different points across the life course may be related to a combination of 28 inflammation markers. (
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