A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Agents that improve the ability to carry out activities such as athletics, mental endurance, work, and resistance to stress. The substances can include PRESCRIPTION DRUGS; DIETARY SUPPLEMENTS; phytochemicals; and ILLICIT DRUGS.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Established cell cultures that have the potential to propagate indefinitely.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins prepared by recombinant DNA technology.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Substances that reduce the growth or reproduction of BACTERIA.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A cell line derived from cultured tumor cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The rate dynamics in chemical or physical systems.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay.
Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.
Warfare involving the use of living organisms or their products as disease etiologic agents against people, animals, or plants.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Biological activities and function of the whole organism in human, animal, microorgansims, and plants, and of the biosphere.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Process that is gone through in order for a drug to receive approval by a government regulatory agency. This includes any required pre-clinical or clinical testing, review, submission, and evaluation of the applications and test results, and post-marketing surveillance of the drug.
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
Stable xenon atoms that have the same atomic number as the element xenon, but differ in atomic weight. Xe-124, 126, 128-131, 134, and 136 are stable xenon isotopes.
The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Drugs that inhibit cholinesterases. The neurotransmitter ACETYLCHOLINE is rapidly hydrolyzed, and thereby inactivated, by cholinesterases. When cholinesterases are inhibited, the action of endogenously released acetylcholine at cholinergic synapses is potentiated. Cholinesterase inhibitors are widely used clinically for their potentiation of cholinergic inputs to the gastrointestinal tract and urinary bladder, the eye, and skeletal muscles; they are also used for their effects on the heart and the central nervous system.
The walnut plant family of the order Juglandales, subclass Hamamelidae, class Magnoliopsida. They are mainly temperate zone trees.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7.
A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.
An aspect of cholinesterase (EC 3.1.1.8).
A hard, brittle, grayish-white rare earth metal with an atomic symbol Ru, atomic number 44, and atomic weight 101.07. It is used as a catalyst and hardener for PLATINUM and PALLADIUM.
Measurement of the intensity and quality of fluorescence.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Exclusive legal rights or privileges applied to inventions, plants, etc.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Any visible result of a procedure which is caused by the procedure itself and not by the entity being analyzed. Common examples include histological structures introduced by tissue processing, radiographic images of structures that are not naturally present in living tissue, and products of chemical reactions that occur during analysis.

Assaying potential carcinogens with Drosophila. (1/4973)

Drosophila offers many advantages for the detection of mutagenic activity of carcinogenic agents. It provides the quickest assay system for detecting mutations in animals today. Its generation time is short, and Drosophila is cheap and easy to breed in large numbers. The simple genetic testing methods give unequivocal answers about the whole spectrum of relevant genetic damage. A comparison of the detection capacity of assays sampling different kinds of genetic damage revealed that various substances are highly effective in inducing mutations but do not produce chromosome breakage effects at all, or only at much higher concentrations than those required for mutation induction. Of the different assay systems available, the classical sex-linked recessive lethal test deserves priority, in view of its superior capacity to detect mutagens. Of practical importance is also its high sensitivity, because a large number of loci in one fifth of the genome is tested for newly induced forward mutations, including small deletions. The recent findings that Drosophila is capable of carrying out the same metabolic activation reactions as the mammalian liver makes the organism eminently suitable for verifying results obtained in prescreening with fast microbial assay systems. An additional advantage in this respect is the capacity of Drosophila for detecting short-lived activation products, because intracellular metabolic activation appears to occur within the spermatids and spermatocytes.  (+info)

Experimental axonal injury triggers interleukin-6 mRNA, protein synthesis and release into cerebrospinal fluid. (2/4973)

Diffuse axonal injury is a frequent pathologic sequel of head trauma, which, despite its devastating consequences for the patients, remains to be fully elucidated. Here we studied the release of interleukin-6 (IL-6) into CSF and serum, as well as the expression of IL-6 messenger ribonucleic acid (mRNA) and protein in a weight drop model of axonal injury in the rat. The IL-6 activity was elevated in CSF within 1 hour and peaked between 2 and 4 hours, reaching maximal values of 82,108 pg/mL, and returned to control values after 24 hours. In serum, the levels of IL-6 remained below increased CSF levels and did not exceed 393 pg/mL. In situ hybridization demonstrated augmented IL-6 mRNA expression in several regions including cortical pyramidal cells, neurons in thalamic nuclei, and macrophages in the basal subarachnoid spaces. A weak constitutive expression of IL-6 protein was shown by immunohistochemical study in control brain. After injury, IL-6 increased at 1 hour and remained elevated through the first 24 hours, returning to normal afterward. Most cells producing IL-6 were cortical, thalamic, and hippocampal neurons as confirmed by staining for the neuronal marker NeuN. These results extend our previous studies showing IL-6 production in the cerebrospinal fluid of patients with severe head trauma and demonstrate that neurons are the main source of IL-6 after experimental axonal injury.  (+info)

A technique for dual determination of cytotoxic and helper lymphocyte precursor frequency by a miniaturized dye release method. (3/4973)

Helper (HTLPf) and cytotoxic (CTLPf) lymphocyte precursor frequency assays are increasingly used in bone marrow stem cell and organ transplant compatibility testing. Current techniques require large cell numbers and radioisotopes. To improve the technique, we developed a miniaturized fluorescent read-out combined HTLPf/CTLPf limiting dilution assay. The assay requires only 5 x 10(6) stimulators, 2 x 10(6) responders and 0.24 x 10(6) target cells in Terasaki plates (40 microl/well). For the HTLPf, culture supernatants from each well were assayed for IL-2 production. The IL-2-dependent proliferation of the mouse 9.12 cell line was detected by a semi-automated fluorescent dye technique. After addition of rhIL-2 (recombinant human IL-2) on days 3 and 7, CTLPs were detected on day 10 by measuring the lysis of dye-labeled targets. Results were comparable to standard radioisotope-based techniques. The assay had a coefficient of variation of approximately 30%. The assay detected helper CD4 cells, pure cytotoxic CD8, helper CD8 cells and helper/cytotoxic CD8 cells. Discrimination was demonstrated between HLA-matched related and non-related pairs. The ease of testing and small cell numbers required should facilitate further evaluation of HTLPf and CTLPf for compatibility testing in unrelated donor transplantation and monitoring immune responses following adoptive transfer of lymphocytes.  (+info)

Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects. (4/4973)

Evolving levels of resistance in insects to the bioinsecticide Bacillus thuringiensis (Bt) can be dramatically reduced through the genetic engineering of chloroplasts in plants. When transgenic tobacco leaves expressing Cry2Aa2 protoxin in chloroplasts were fed to susceptible, Cry1A-resistant (20,000- to 40,000-fold) and Cry2Aa2-resistant (330- to 393-fold) tobacco budworm Heliothis virescens, cotton bollworm Helicoverpa zea, and the beet armyworm Spodoptera exigua, 100% mortality was observed against all insect species and strains. Cry2Aa2 was chosen for this study because of its toxicity to many economically important insect pests, relatively low levels of cross-resistance against Cry1A-resistant insects, and its expression as a protoxin instead of a toxin because of its relatively small size (65 kDa). Southern blot analysis confirmed stable integration of cry2Aa2 into all of the chloroplast genomes (5, 000-10,000 copies per cell) of transgenic plants. Transformed tobacco leaves expressed Cry2Aa2 protoxin at levels between 2% and 3% of total soluble protein, 20- to 30-fold higher levels than current commercial nuclear transgenic plants. These results suggest that plants expressing high levels of a nonhomologous Bt protein should be able to overcome or at the very least, significantly delay, broad spectrum Bt-resistance development in the field.  (+info)

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (5/4973)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Enterotoxin-producing bacteria and parasites in stools of Ethiopian children with diarrhoeal disease. (6/4973)

Enterotoxinogenic bacteria were isolated from 131 (37%) of 354 Ethiopian infants and children with acute gastrointestinal symptoms. Only one of these isolates belonged to the classical enteropathogenic serotypes of Esch. coli. Two colonies from each patient were isolated and tested for production of enterotoxin by the rabbit ileal loop test, the rabbit skin test, and an adrenal cell assay. However, only 38% of the isolated enterotoxinogenic strains were Esch. coli; the others belonged to Klebsiella, Enterobacter, Proteus, Citrobacter, Serratia, and Aeromonas. In 18 patients both isolates were toxinogenic and belonged to different species. The incidence of intestinal parasites was 35% with no apparent correlation to the occurrence of toxinogenic bacteria in the stools.  (+info)

Animals as sentinels of human health hazards of environmental chemicals. (7/4973)

A workshop titled "Using Sentinel Species Data to Address the Potential Human Health Effects of Chemicals in the Environment," sponsored by the U.S. Army Center for Environmental Health Research, the National Center for Environmental Assessment of the EPA, and the Agency for Toxic Substances and Disease Registry, was held to consider the use of sentinel and surrogate animal species data for evaluating the potential human health effects of chemicals in the environment. The workshop took a broad view of the sentinel species concept, and included mammalian and nonmammalian species, companion animals, food animals, fish, amphibians, and other wildlife. Sentinel species data included observations of wild animals in field situations as well as experimental animal data. Workshop participants identified potential applications for sentinel species data derived from monitoring programs or serendipitous observations and explored the potential use of such information in human health hazard and risk assessments and for evaluating causes or mechanisms of effect. Although it is unlikely that sentinel species data will be used as the sole determinative factor in evaluating human health concerns, such data can be useful as for additional weight of evidence in a risk assessment, for providing early warning of situations requiring further study, or for monitoring the course of remedial activities. Attention was given to the factors impeding the application of sentinel species approaches and their acceptance in the scientific and regulatory communities. Workshop participants identified a number of critical research needs and opportunities for interagency collaboration that could help advance the use of sentinel species approaches.  (+info)

Inhibitory effect of plasma obtained from hypophysectomized and control women on the assay of bioactive luteinizing hormone. (8/4973)

The purpose of this study was to determine the effect of components of female plasma on the value of bioactive luteinizing hormone (LH), especially in the presence of low immunological LH value. Using both an immunoradiometric assay (IRMA) and rat Leydig cell bioassay, immunoreactive (I) and bioactive (B) LH were assessed in plasma collected from women during a gonadotrophin releasing hormone (GnRH) test performed on day 7 of a spontaneous cycle. Two modes of response to an acute administration of GnRH were defined: normal production of gonadotrophins (group I) and excessive secretion (group II) associated with a significant difference in the B/I-LH ratio between the two groups. The B/I-LH ratio did not vary with sampling time during the test in either group. The addition of LH-free plasma collected from hypophysectomized women caused a 30% decrease in testosterone production compared to control values (in the presence or absence of hLH standard). A partial restoration of testosterone production was observed if plasma was first treated with PEG 12%. The inhibitory factor(s) was also present in plasma from ovulatory women, even after treatment by an antibody against the entire LH molecule. The effect of normal (A) or low I-LH plasma (B) on testosterone production varied strongly according to the plasma volume added to the bioassay, as well as to plasma treatments. Diethylether treatment caused a 50% decrease in testosterone secretion for plasma B (but not for A) whereas a diminution of the steroidogenesis is observed after a PEG treatment of plasma A (but not for B), suggesting that different inhibitory factors are present in plasmas A and B. Therefore the LH bioactivity measured in the rat Leydig cell assay, in terms of testosterone output, seems to represent a balance between the LH molecule and the presence of inhibitory factors in the plasma.  (+info)

Records the work and recommendations of an expert committee concerned with establishing and maintaining international standards and reference reagents for selected biologicals. Sections announce substances newly accepted as international standards or reference reagents, those replacing previous ones, and substances newly selected for collaborative studies. Annexed to the report are extensive sections on the standardization of interferons and amendments to WHO requirements No. 8-10 for diphtheria toxoid, pertussis vaccine, tetanus toxoid, and combined vaccines. Other annexes cover revised requirements for hepatitis B vaccine prepared from plasma and new requirements for live varicella ...
A green fluorescent protein based recombinaant cell bioassay for the detection of activators of the aryl hydrocarbon receptor: application for screening of a 1,
Copper Colorimetric Assay Kit, QuantiChrom from BioAssay Systems,One-step, microplate or cuvet, colorimetric, linear detection range 8 mg/dL to 300 mg/dL. Procedure: 5 min.,biological,biology supply,biology supplies,biology product
Uric Acid Colorimetric Assay Kit, QuantiChrom from BioAssay Systems,One-step, microplate or cuvet, colorimetric, linear detection range 0.2 mg/dL to 20 mg/dL. Procedure: 20 min.,biological,biology supply,biology supplies,biology product
BioAssay Systems Phospolipid Assay Kit (EPLP-100) measures choline-containing phospholipids in biological samples. The absorbance at 570nm or fluorescence intensity at 530/585 nm is proportional to the phospholipid concentration. Detection range: 3 - 200 µM (colorimetric), 0.6 - 20 µM (fluorimetric).
BioAssay Systems salicylate detection kit provides a convenient and reliable means to measure salicylate. In the assay, salicylate complexes with ferric chloride to create a colored compound that can be measured at 560 nm. This assay can be used with a variety of samples and is simple, sensitive, and adaptable to high-throughp
Cylinder Plate Method Close bracketing gives more accurate results. Examples include contractions of muscle, blood pressure, blood sugar concentrations, etc.  Establishing regulatory requirements for water quality;  Ecological monitoring of sewages discharge. Methods of Bioassay for Agonists Bioassay of Antagonists Bioassay of some important drugs Current status of Bioassay in different Pharmacopoeia Other Official Bioassays Keywords Bioassay, official method, adrenaline, digitalis, d-tubocurarine, heparin, insulin, oxytocin, gonadotropin, chorionic gonadotropin, urokinase, streptokinase,diphtheria vaccine, plague vaccine 1. Dose can be plotted even if it varies over thousand fold range. Absorb excess solution immediately by a tip of filter paper and then allow the particles to evaporate the solvent for about 30 min at room temperature. Amount of test drug available is small, does not involve complicated calculations and it does not depend on Dose response curve [170-183]. ...
Cover by D.Meissner WHO/GRA These Guidelines were adopted by the WHO Expert Committee on Biological Standardization at its 59th meeting which took place in Geneva…
Journal Article 2016; 37(Suppl 1): 17-24 PubMed PMID: 28263526 Citation Keywords: Animals, Biological Assay:methods, Environmental Monitoring, Fishes, Toxicity Tests:methods, Waste Water:toxicity, Water Pollutants, Chemical:toxicity,. : Wastewater, especially containing hospital effluents, exhibits high chemical complexity and specificity since it includes various chemicals, biocides, pharmaceuticals, surfactants, radionuclides, disinfectants and pathogens. Biological tests provide true evidence of the wastewater quality and unlike chemical analytical tests show comprehensive pollution effects on the environment and human health. Normalized conventional bioassays are not sensitive enough for ecotoxicological evaluation of wastewater and there is a great need for the development of suitable sensitive bioassays in order to characterize properly the residual toxicity of treated effluents. Provisions of binding EU legislation regarding protection of animals used for scientific purposes and ...
Plasma samples obtained from patients receiving a 24-h continuous infusion of human recombinant (hr)TNF or a combination of two overlapping, 24-h continuous infusions of hrIFN-gamma and hrTNF were analyzed for IL-6 in a sensitive bioassay. A transient appearance of circulating IL-6 was observed with peak levels between 3 and 6 h after the start of the hrTNF infusion. These peak levels correlated quite well with the dose of hrTNF administered (r = 0.86; p less than 0.001). The maximal value observed was 27.5 ng/ml IL-6 in a sample of a patient receiving 545 micrograms/m2 hrTNF. The combination of hrIFN-gamma (200 micrograms/m2) and hrTNF in the infusions resulted in higher IL-6 levels than a comparable dose of hrTNF alone. A maximal value of 23.5 ng/ml IL-6 was observed in a patient receiving 205 micrograms/m2 hrTNF. No IL-6 was found in the plasma of patients during the 12-h infusion with hrIFN-gamma alone, except for two borderline samples. ...
Potency testing is critical for the regulatory approval and lot release of biologics. In particular, FDA and EMA guidelines mandate that customized potency assays be developed and validated prior to clinical trials. This testing is commonly done through in vivo or in vitro bioassays, as advised by global regulatory agencies, to demonstrate product efficacy and manufacturing consistency. Because potency assays are essential in verifying product quality, it is critical to choose a testing partner that understands your development needs ...
Potency testing is critical for the regulatory approval and lot release of biologics. In particular, FDA and EMA guidelines mandate that customized potency assays be developed and validated prior to clinical trials. This testing is commonly done through in vivo or in vitro bioassays, as advised by global regulatory agencies, to demonstrate product efficacy and manufacturing consistency. Because potency assays are essential in verifying product quality, it is critical to choose a testing partner that understands your development needs ...
Preclinical CRO for pharmacology, dermatology and cosmetics, in vitro models and bioassays alternative methods to animal testing.
TY - JOUR. T1 - Soluble CD141-152 confers responsiveness to both lipoarabinomannan and lipopolysaccharide in a novel HL-60 cell bioassay. AU - Yu, Weiming. AU - Soprana, Elisa. AU - Cosentino, Giovanna. AU - Volta, Manuela. AU - Lichenstein, Henri S.. AU - Viale, Giovanna. AU - Vercelli, Donata. PY - 1998/10/15. Y1 - 1998/10/15. N2 - CD14 is a pattern recognition receptor involved in the interaction with multiple ligands, including LPS from Gram-negative bacteria and lipoarabinomannan (LAM) from mycobacteria. While the interactions between LPS and soluble CD14 (sCD14) have been analyzed in detail, LAM/CD14 interactions remain uncharacterized due to the lack of suitable functional assays. We describe herein a novel bioassay for the analysis of CD14/ligand interactions. CD14-negative myeloid HL-60 cells up-regulate endogenous CD14 gene expression when stimulated with LPS in the presence of recombinant soluble CD141-348. Using the HL-60 bioassay, we showed that sCD141- 348 confers responsiveness ...
β-Cell injury plays an important role in the development of type 1 and type 2 diabetes. Most of the β-cell bioassays depend on labeling or endpoint assessments that might not capture the true physiology or pathology of the injury process. In the current study, we dynamically detected a broad range of pathological and pharmacological responses to four toxicants (cytokine mixture, free fatty acid mi ...
BBI SolutionsBBI solutions為一家領先的開發商以及原料/成品測試製造商,目前已於五個不同的國家設立據點,生產的產品包括:生物原料:抗原、酶、monoclonal / 重組的Fab antibody血清和血漿產品:正常人血清及患者樣本,疾病狀態離子另外還有金奈米粒子及Horseraddish過氧化物及葡萄糖氧化物的生產。BioAssay SystemsBioAssay Systems 公司從事研發和銷售創新高通量的檢測試劑組,以滿足不斷增加需求的生命科學產業。提供的生物測定系統檢測試劑盒,使用簡單方便且性能優越。BioAssay Systems 著重於安全、無放射性、吸收熒光和發光檢測技術,目前的產品包括以下幾個方面:*檢測試劑盒及試劑»血液和尿液化學»代謝/酶活性»陰離子和陽離子»氧化激活»信號轉導»HTS試劑*設備及配件»手持式讀寫器»酶標儀»微孔板*服務»分析服務»含量定制/設計與開發Bioassay Technology LaboratoryBT
Supplier: BioAssay Systems Product Type: Bioassay kit Product Description: Quantitative determination of acetylcholine by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 30 min. Kit size: 100 tests. Detection limit: colorimetric assay 10 µM, fluorimetric assay 0.4 µM. Shelf life: 3 months. Shippi
C55 High throughput screening technologies have revolutionized the manner in which potential therapeutics are identified. One casualty of the move to high throughput screening technologies has been the widespread disappearance of natural product extracts from pharmaceutical screening programs. Though they are the source of lead compounds for ~65% of anticancer and antimicrobial drugs approved by the FDA between 1981-2002, natural products have often been excluded from modern screening programs. This is due, at least in part, to the inherent difficulties in testing complex extract mixtures, which often contain nuisance compounds, in modern bioassay systems. Here we present a novel electrochemiluminescent assay system that is suitable for testing natural product extracts in high throughput screening systems. To demonstrate its utility, we have developed a screen for inhibition of E3 (ubiquitin ligase) activity utilizing the cancer-relevant molecular target MDM2. Additional screens for inhibition ...
Assay Development: Fundamentals and Practices by Ge Wu 429 pages March 2010 Description Essential principles and practice of assay development The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an as
Stuckey is a expert on anaerobic digestion and has developed both the novel Anaerobic Baffled Reactor (ABR), and the Submerged Anaerobic Membrane Bioreactor (SAMBR). The latter was awarded the Royal Society Brian Mercer Award for Innovation in 2008. Over the years he has developed the standard bioassay techniques used in the field and is internationally leading in the production and characterisation of Soluble Microbial Products (SMPs) in anaerobic systems. Role: Stuckey lab will focus on the scaling up of developed synthetic communities and transfer of insights from natural communities to applications in industrial reactors. Stuckey Lab ...
Hi For the last 8 months Ive been working on an in vitro bioassay for FSH. However, I have been struggling with a lack of sensativity when dealing with serum samples which are typically around the 2 ng/ml region. The assay involves incubating sertoli cells from the testes of 7-10 day old rats in the presence of FSH. The presence of FSH then causes a dose-dependent increase in aromatase activity when adrostendione is converted to estradiol which can be measured to determine the concentration of FSH. If anyone out there is familiar with this assay and can help me to increase the sensativity (by decreasing the variability) then please e-mail me Wayne Young Youngwa at agresearch.cri.nz AgResearch New Zealand ...
CHI s Bioassays for Immuno-Oncology Symposium, October 23, 2017, Washington, DC, brings together experts in bioassays for immuno-oncology therapies will discuss selection, development, and standards for bioassays and immunoassays. Special attention will be given to understanding the mechanism-of-action for immunotherapies, whether they be antibody- or cell-based. Overall, this one-day immersive symposium will outline a product life cycle approach for developing and implementing biological assays from preclinical studies to clinical development. This symposium is part of the Immunogenicity & Bioassay Summit.
A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. A reaction well containing a sample portion, donor reagent, and acceptor reagent and a matrix well containing a sample portion and donor reagent are excited and the resulting emission from each is measured at a single wavelength associated with the acceptor. The measurement obtained from the matrix well is used to provide a correction for the measurement obtained from the reaction well. The sample may be a biological fluid such as an oral fluid.
In general, all constituents of a multi-component compound are not equally important for the process in hand. If we restrict ourselves to some sort of biological assay or property measure such as logD or pKa, then these are easily understood as reflecting the presence of a parent molecule. Data such as aD reflect the presence of isomeric parent molecule. Note that neither of these parent molecules have an independent existence as a physical entity, in the Daylight sense they are identifiers or names, about which, data exist. Data such as melting point are clearly about a particular sample, as are the experimental results of both physicochemical and biological assays. A version molecule structure may also have been assigned to this sample perhaps a salt or solvate or even a impure isomer.. Calculated data such as cLogP are about the parent molecule, whereas a rubicon structure is a datum about the isomeric parent. An x-ray structure on the other hand is a datum about the sample, and is related to ...
Secondly we were interested in the amount and distribution of PrPSc at 24 mpi, since the extent of this accumulation varied at earlier time points with peaks at 8 and in particular 12 mpi and lows at 16 mpi respectively [10]. However, these earlier studies did also suggest a higher amount in animals at 24 mpi suggesting an undulant pattern of about 12 months. This finding is now supported by the bioassay results, as three out of the four cattle from the 24 mpi group showed levels and distribution of infectivity comparable to the peaks seen at 12 mpi. However it has to be bear in mind that only four animals per time point were investigated here and in earlier studies and that the variations between individuals are very high [10,14]. This is reflected in the present study by variable detection rates in different animals of the 24 mpi group and might explain the differences seen in infectivity levels reported for ileal samples by several authors before [6,9-11,15]. In summary, data presented here ...
Secondly we were interested in the amount and distribution of PrPSc at 24 mpi, since the extent of this accumulation varied at earlier time points with peaks at 8 and in particular 12 mpi and lows at 16 mpi respectively [10]. However, these earlier studies did also suggest a higher amount in animals at 24 mpi suggesting an undulant pattern of about 12 months. This finding is now supported by the bioassay results, as three out of the four cattle from the 24 mpi group showed levels and distribution of infectivity comparable to the peaks seen at 12 mpi. However it has to be bear in mind that only four animals per time point were investigated here and in earlier studies and that the variations between individuals are very high [10,14]. This is reflected in the present study by variable detection rates in different animals of the 24 mpi group and might explain the differences seen in infectivity levels reported for ileal samples by several authors before [6,9-11,15]. In summary, data presented here ...
Enzymes are crucial for a variety of cellular functions (e.g. metabolism, signal transduction). Enzymatic activity assays measure enzyme kinetics and inhibition, by different detection methods (e.g. absorbance, fluorescence, or chemiluminescence).
The NanoBRET™ Target Engagement (TE) Assay measures compound binding at select target proteins in intact mammalian cells. The assay uses bioluminescence energy transfer (BRET), achieved by transferring the luminescent energy from NanoLuc® luciferase to the fluorescent tracer that is bound to the target protein-NanoLuc® fusion, to directly measure compound permeability, binding affinity and compound-target residence time.
Clonogenic assays measure the ability of single cells to proliferate and form a colonyThis process approximates closely the regrowth and recurrence of tumors after treatment with radiation or chemotherapyproviding an assay to screen for drugs that block this processYetclonogenic assays are labor intensive and too cumbersome for high throughout screeningHTSof compound librariesHere we propose to de .... ...
PIPETMAX® is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that ...
ZenBio offers a variety of assays to using our ArrayScan XTI. Customers can choose from any of the following assays or we can design a custom program tailored to each clients specific research needs.
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Annex 1: Release procedure of International Chemical Reference Substances; Annex 2: WHO good practices for pharmaceutical microbiology laboratories; Annex 3: WHO good manufacturing practices: main principles for pharmaceutical Products; Annex 4: WHO good manufacturing practices for blood establishments (jointly with the Expert Committee on Biological Standardization); Annex 5: WHO guidelines on good manufacturing practices for heating, ventilation and air-conditioning systems for non-sterile pharmaceutical dosage forms; Annex 6: WHO good manufacturing practices for sterile pharmaceutical products; Annex 7: WHO guidelines on transfer of technology in pharmaceutical manufacturing; Annex 8: Good pharmacy practice: standards for quality of pharmacy services (joint FIP/WHO); Annex 9: Model guidance for the storage and transport of time- and temperature-sensitive pharmaceutical products (jointly with the Expert Committee on Biological Standardization); Annex 10: Procedure for prequalification of ...
Looking for online definition of biologic assay in the Medical Dictionary? biologic assay explanation free. What is biologic assay? Meaning of biologic assay medical term. What does biologic assay mean?
Intended Use. For the quantitative in vitro determination of Lp(a) in serum or plasma. This product is suitable for automated, semi-automated and manual use.. Clinical Significance. Lipoprotein (a) determination is intended for use in conjunction with clinical evaluation, patient risk assessment and other lipid tests to evaluate disorders of lipid metabolism and to assess coronary heart disease in specific populations.. WHO/IFCC Reference Material. The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), through its Working Group on Lp(a) and together with research institutions and several diagnostic companies recommends that laboratories use assays which do not suffer from apo(a) size-related bias. The IFCC SRM 2B was accepted by the WHO Expert Committee on Biological Standardisation as the First WHO/IFCC International Reference Reagent for Lp(a) to ensure conformity by diagnostic companies to the European Union?s Directive on In vitro Diagnostic Medical Devices for ...
Intended Use. For the quantitative in vitro determination of Lp(a) in serum or plasma. This product is suitable for automated, semi-automated and manual use.. Clinical Significance. Lipoprotein (a) determination is intended for use in conjunction with clinical evaluation, patient risk assessment and other lipid tests to evaluate disorders of lipid metabolism and to assess coronary heart disease in specific populations.. WHO/IFCC Reference Material. The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), through its Working Group on Lp(a) and together with research institutions and several diagnostic companies recommends that laboratories use assays which do not suffer from apo(a) size-related bias. The IFCC SRM 2B was accepted by the WHO Expert Committee on Biological Standardisation as the First WHO/IFCC International Reference Reagent for Lp(a) to ensure conformity by diagnostic companies to the European Union?s Directive on In vitro Diagnostic Medical Devices for ...
Volledige genoomsequentie van een nieuw vispapillomavirus gedetecteerd in gekweekte meerval (Silurus glanis) Een nieuw papillomavirus (PV) werd gedetecteerd in gekweekte meerval (Silurus glanis) in Hongarije en vertoonde klinische symptomen die lijken op die van de ziekte van wels meerval herpesvirus. Het hele genoom van Silurus glanis papillomavirus 1 (SgPV1) werd geïdentificeerd met behulp van next-generation sequencing. Het volledige genoom […]. ...
Video articles in JoVE about bioassay include Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine, A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors, A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms, Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles, A Rapid In Vivo Bioassay for Developmentally Active Enhancers, A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry, Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators, Bone Conditioned Medium: Preparation and Bioassay, Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays, Screening for Endocrine Activity in Water Using Commercially-available In Vitro
The effects of many chemicals on cellular processes are governed by their ability to enter the cell, which is in turn a function of the composition of the cells external environment. To examine this relationship, the effect of serum in cell culture medium on the bioavailability of cytochrome P450 1A (CYP1A)-inducing compounds was determined in PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cells. The presence of 10% calf serum in the medium increased the EC50 (effective concentration to achieve 50% maximal response) for induction of ethoxyresorufin O-deethylase (EROD) activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) 20-fold as compared to treatment in serum-free medium. Measurement of [3H]TCDD uptake and Ah receptor binding indicated that the apparent difference in potencies was a result of decreased bioavailability in the presence of serum, effectively reducing the concentration of TCDD within the cells. Induction of EROD and CYP1A protein in response to treatment with each of ...
Host Cell Protein Assay Development. After polyclonal antibody generation, Rockland performs assay development for clients who choose to add that service. Assay development is customized to each individual client. Client can decide to stop the project at the end of multiple project phases. Below is an example of an assay development project for a capture sandwich ELISA that was performed recently by Rockland Immunochemicals.
Several of the pressing scientific challenges in the filed of environmental toxicology are to understand the basic mechanisms of chemically-induced toxic substances and of the design, interpretation, and extrapolation of data from non-human bioassay systems to the human situation. The objective is to offer a new paradigm, namely the idea that cytotoxic and epigenetic effects of chemicals can contribute to many toxic endpoints in human beings. We will speculate that the disruption of calcium homeostasis by a wide variety of chemicals and the conditions of their use might provide an unification of mechanisms leading to either cytotoxicity or epigenetic changes. Depending on the conditions, the effects of this disruption, a pleiotropy of diseases might result, namely, embryotoxicity, teratogenesis, tumor promotion, immune modulation, neurotoxicity, and preproductive dysfunction. In summary, there are at least three biological endpoints at the cell level which chemicals can influence - mutations, cell death
Profiling Animal Toxicants by Automatically Mining Public Bioassay Data: A Big Data Approach for Computational Toxicology Jun Zhang 1,2, Jui-Hua Hsieh 3, Hao Zhu 1,2 * 1 Department of Chemistry, Rutgers
Khan, S. M. M. K., Yoshimura, C., Arikawa, M., Omura, G., Nishiyama, S., Suetomo, Y., Kakuta, S., Suzaki, T. 2006 Axopodial degradation in the heliozoon Raphidiophrys contractilis: a novel bioassay system for detecting heavy metal toxicity in an aquatic environment. Environ. Sci., 13, 193-200 ...
Menendez will present a lecture outlining sensible approaches to developing reproducible bioassays using live systems and/or biologically derived reagents. Additionally, Menendez will conduct a workshop on writing validation protocols, and will give participants hands-on experience and case studies, which will add relevance to lecture material as well as create a point of discussion. Targeted audiences are bio-analytical scientists, validation specialists, project management leaders and bioassay scientists involved in bioassay execution. Upon completion of the course, participants will receive a certificate of attendance.. Bioassays are necessary components of the biopharmaceutical development process as functional potency assays during release, stability and formulation studies. The course expands on the importance of the appropriate use of non-analytical techniques that are critical during PK/PD evaluation and measurement of systematic responses to drugs, such as immunogenicity. Also included ...
Overall, the company believes there is alignment with the FDA on its panel of analytical methods and the proposed assay qualification/validation plans. Analytical methods are used to assess how reliably and consistently the key product characteristics can be determined in order to ensure patients receive safe and effective product.. In the meeting minutes, the FDA made a request that the company complete implementation of its potency assay qualification plan, presented in the meeting, prior to initiation of upcoming clinical studies. This assay utilizes the Delta 7 mouse model, which has been used historically to assess AVXS-101 potency but now incorporates additional elements to make it acceptable to global regulatory authorities. Specifically, the agency stated, We believe that your potency assay development and qualification plans have the potential to result in an acceptable potency assay, and we are encouraged that the assay includes reference controls that allow for calculation of ...
Pharmaceutical Information » Question Category » Education & Reference » Pharmacology » Test microorganism used in the microbiological assay of Streptomycin ...
I love CATs too there so CUTE, Rodent bioassays are also costly, espe- cially if the burn the midnight oil intrigue includes review of the toxicokinetic properties of the assay substance, or microarray studies if mechanistic statistics is needed to clean neoplasm responses. Not every cable company is charging into the market. Linense, Conil y Pozoblanco) se han marchado sin haber sido derrotados. Of these, cheapest avodart the sexual side effects are perhaps the most extensively reported. The trial is currently ongoing, and Histogen expects to release full results from this first clinical study in the first half of 2009. OFF 1 DOSE on a small area of scalp. New conservative prime minister Tony Abbott, who won his election campaign on the back of a strong anti-immigration stance, meets with Indonesian president Susilo Bambang Yudhoyono in Jakarta on Monday. We like the truth that you can run in this trial, which is appropriate to the product and also is seldom seen in Virtual Reality experiences. ...
The project reviews the methods used for microbial analyses, determine the microbiological methods currently used by the reclaimed water industry, develop a systematic process for selecting appropriate methods, and develop a framework for round-robin testing of microbiological methods by the industry.
Ensure CMC Success and Expedite Your Products Path to Market by Optimizing Characterization Strategies and CMC Bioassay Development. Gain Practical Advice From Case Studies, Regulatory Interactions And Perspectives, And Emerging Technologies To Accelerate Your Biologics to Market THE LEADING EVENT TO ENSURE CMC, REGULATORY SUCCESS AND EXPEDITED PRODUCT APPROVAL Gain Practical Advice From Case Studies, FDA & Regulatory Leaders to Accelerate Your Biologics & Novel Modalities/Molecules to Market
Filtering by: Keyword nucleic acid hybridization Remove constraint Keyword: nucleic acid hybridization Keyword analysis Remove constraint Keyword: analysis Keyword biological assay Remove constraint Keyword: biological assay Rights http://rightsstatements.org/vocab/InC/1.0/ Remove constraint Rights: http://rightsstatements.org/vocab/InC/1.0/ ...
BioAssays organized into assay projects based on linkage to Summary assays. Click an project below to retrieve the bioassay list for the project. Click All Assay Projects to retrieve all assay projects represented by Summary assays ...
A main presentation track at SLAS 2018 focuses on Assay Development and Screening with emphasis on methods such as high throughput screening, phenotypic screening, and 3rd and 4th dimension screens of cell-based assays.
Course organised by LCBU and gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course
Gain assistance and guidance as you work through extensive and highly complex processes regarding regulations and documentation. Seek proper testing based on class I, II and III devices, increasing the reliability of claims made - while reducing overall risk.
Two industry leading experts have partnered together to take Assay Development to the next level. Lampire Biological Labs and Saladax Biomedical have partnered together to bring you Assay Development, from Concept to Completion. All projects are completed in GMP facilities certified to ISO 13485. We are the only stop you need for your Assay Development. From Pre-Clinical, and Companion Diagnostics, to characterized antibodies and ELISAs, this partnership fills every need you have for your next custom assay. With our expert technical staffs collaborating with you, your next project has to be a success. Dont piece together an assay, get it all at one stop, with our Concept to Completion Assay Development. We look forward to your success!. ...
High quality, fast delivery and ready to use. Our custom solutions for PCR assays development provides the same high quality and unique abilities as our standard PCR assays. Contact us today for a quote.
Hiyoshi began a joint study with XDS in 1998 and came to a licensing agreement in 2000. Since then, Hiyoshi has been working to promote the CALUX Assay in Japan and in Asia. Meanwhile, we have participated in many joint studies with governmental institutes, universities and private companies to prove the superiority of the CALUX Assay ...
Biological assays, or bioassays, are powerful tools that allow scientists to determine the effects of a given substance on living organisms. In this inquiry-based lab, students plan and perform a plant bioassay to determine the environmental hazard of common point and non-point source pollutants. The results are analyzed using averages, standard deviations, and TC50 calculations, integrating STEM ...
Three pressor amines, namely, epinephrine, 3,4-dihydroxyphenethyl-methylamine and 3,4-dihydroxyphenethyldimethylamine were tested for relative potencies by an increasing dose method, and the data were studied to determine when linear relationships might be assumed. The following conclusions may be drawn:. 1. The Weber-Fechner law may be said to hold for restricted ranges of our data but not for the data as a whole.. 2. The application of a progressively increasing dose method to the biological assay of epinephrine may be an improvement upon the U.S.P.XI procedure.. 3. Expressing relative pressor potencies on the basis of doses required to elicit a specific response may not convey a true picture when the characteristic slopes of the logarithmic dose-response curves of two compounds are not identical.. 4. When the logarithmic curves of compounds being compared with epinephrine are dissimilar, it may indicate the presence of qualitative differences in action.. ...
The decimal form should be used when a fraction is given with an abbreviated unit of measure (eg, 0.5 g, 2.7 mm) to reflect the precision of the measurement (eg, 38.0 kg should not be rounded to 38 kg if the scale was accurate to tenths of a kilogram). (See also , Units of Measure, Use of Numerals With Units, Decimal Format.)The patient was receiving gentamicin sulfate, 3.5 mg/kg, every 8 hours. Her serum gentamicin level reached a peak of 5.8 μg/mL and a trough of 0.7 μg/mL after the third dose. Place a zero before the decimal point in numbers less than Less ...
animal bioassays could create a lot of controversy with associations such as PETA. a similar association came to my university once and tried to steal cows that were used for digestive processes experiments. even though it might not seem to relevant, i think it is something important to consider when designing an experiment with animals, you never know what these radical associations can make, and ruin years of experiments ...
the patient population who may be at high risk for disease progression. In the absence of these biologic assays, lack of disease progression is an important end point and, if quantified, may be the best indicator that TKI therapy is really impacting. ...
This course is designed for all individuals who develop, validate, perform or monitor bioassays or immunoassays. Individuals who develop assay methodologies, assign computational and analysis criteria, measure and validate performance characteristics, perform, evaluate and troubleshoot problem assays, or are responsible for the quality of the data will benefit from the lessons and examples presented. Attendees should have a basic understanding of bioassays or immunoassays.
Whether custom conjugation of a ligand to one of our nanoparticles, sample analysis, or full-scale assay development Cytodiagnostics is here to help.Our highly trained technical staff has many years of experience with nanoparticle synthesis, surface modification, analysis, and assay development. This allows us not only
9781574444537 Our cheapest price for Cell-based Bioassays in Drug Discovery And Development is $59.48. Free shipping on all orders over $35.00.
The IL-23 Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to stimulate or inhibit interactions with the IL-23 receptor (IL-23R).
Equipment for collection and concentration of samples from air, surfaces, and liquids for use with rapid microbiological methods ...
Assay in biological systems[edit]. The assay of superoxide generated in biological systems is a difficult task because of its ... Hydrogen peroxide is then assayed by a fluorimetric method.[11] As a free radical, superoxide has a strong EPR signal, and it ... "A fluorimetric assay for hydrogen peroxide, suitable for NAD(P)H-dependent superoxide generating redox systems". Anal Biochem ... high reactivity and short half-life.[11] One approach that has been used in quantitative assays converts superoxide to hydrogen ...
Methods of Biological Assay, 1928; Recent Advances in Materia Medica, 1931; Biological Standardization, 1937; Background of ...
Fisher, R. A. (1949). "A Biological Assay of Tuberculins". Biometrics. 5 (4): 300-316. doi:10.2307/3001513. hdl:2440/15259. ... "Statistical Appendix to a Paper by J. Davidson on Biological Studies of Aphis rumicis". Annals of Applied Biology. 9: 142-145. ... "The Case of Zero Survivors in Probit Assays". Annals of Applied Biology. 22: 164-165. 1935. doi:10.1111/j.1744-7348.1935. ... Fisher, R. A. (1931). "The Evolution of Dominance". Biological Reviews. 6 (4): 345-368. doi:10.1111/j.1469-185X.1931.tb01030.x ...
"Protein-binding assays in biological liquids using microscale thermophoresis". Nature Communications. 1 (7): 100. Bibcode: ... In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose ... Ligand efficacy refers to the ability of the ligand to produce a biological response upon binding to the target receptor and ... These privileged elements[29] can be used as a basis for designing new active biological compounds or compound libraries. ...
Determined by consideration of all biological assay methods (circa 1970):[17]. Androgen. Potency (%). ... Biological activity[edit]. Androgens bind to and activate androgen receptors (ARs) to mediate most of their biological effects. ... Biological function[edit]. Male prenatal development[edit]. Testes formation[edit]. During mammalian development, the gonads ... The Journal of Biological Chemistry. 280 (9): 7786-92. doi:10.1074/jbc.M413992200. PMID 15623502.. ...
The sensor is also available as tape.[14] Other methods have been developed to assay biological hydrogen production. [15] ...
"A sensitive microbiological assay procedure for determining magnesium in biological materials". The Analyst. 88 (1048): 534. ... "Society of Biological Chemists, India. 2017. Archived from the original on 24 February 2017. Retrieved 23 February 2017.. ... The Society of Biological Chemists (India) awarded him the Professor M. Srinivasayya Award in 1984[21] and the Indian National ...
"All-Optical Assay to Study Biological Neural Networks". Frontiers in Neuroscience. 12: 451. doi:10.3389/fnins.2018.00451. PMC ... A new class of fluorescent indicators". The Journal of Biological Chemistry. 272 (20): 13270-4. doi:10.1074/jbc.272.20.13270. ... biological degradation and toxicity, in addition to higher signal-to-noise ratios. Such systems may rely on aequorin and the ... The Journal of Biological Chemistry. 279 (14): 14280-6. doi:10.1074/jbc.M312751200. PMID 14742421. Mank M, Reiff DF, Heim N, ...
Afshar Saber W, Gasparoli FM, Dirks MG, Gunn-Moore FJ, Antkowiak M (2018). "All-Optical Assay to Study Biological Neural ...
Enhanced chemiluminescent assay for antioxidant capacity in biological fluids. Analynca Chumca Acta, 266, 265-277 Sawcer, K.E ... Eclox ECL is a broadband enhanced chemiluminescence (ECL) assay that can be used to qualitatively assess a water sample to ... Reliability of the Eclox Enhanced Chemiluminescence Assay for Rapid Field Testing of Drinking Water, Thesis, Texas A&M ...
Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (2010). "Protein-binding assays in biological liquids using microscale ... "Assay and Drug Development Technologies. 9 (4): 342-53. doi:10.1089/adt.2011.0380. PMC 3148787. PMID 21812660.. ...
... in biological assays refer to artifacts in data that are caused by the position of the wells on a screening plate ... rather than a biological effect.[citation needed] The edge effect in scanning electron microscopy is the phenomenon in which ... in the environmental conditions that result from the proximity to a structurally dissimilar matrix Direct biological effects- ... Changes in species abundance and distribution caused directly by physical conditions near the edge Indirect biological effects ...
Biological Assays of Various Synthetic Compounds". Science. 88 (2271): 38-39. doi:10.1126/science.88.2271.38. PMID 17780891. ... Journal of Biological Chemistry. 113 (1): 319-332. Evans, H. M.; Emerson, O. H.; Emerson, G. A. (2009). "THE ISOLATION FROM ...
... allows Bradford assay to be used for fingerprint analysis. Bradford assay was successfully used to identify the biological sex ... and having little to no assay preparation, in comparison to the Ninhydrin chemical assay which requires assay preparation such ... The Bradford assay uses the spectral properties of Coomassie Brilliant Blue G-250 to estimate the amount of protein in a ... Compton, S. J.; Jones, C. G. (1985). "Mechanism of dye response and interference in the Bradford protein assay". Analytical ...
"Lipid G Protein-coupled Receptor Ligand Identification Using β-Arrestin Path Hunter™ Assay". Journal of Biological Chemistry ... "The Journal of Biological Chemistry. 265 (3): 1454-8. PMID 2104842.. *^ Belkner, J; Stender, H; Kühn, H (1998). "The rabbit 15- ... "Journal of Biological Chemistry. 285 (29): 22211-20. doi:10.1074/jbc.M110.119982. PMC 2903350. PMID 20460374.. ... "The Journal of Biological Chemistry. 273 (36): 23225-32. doi:10.1074/jbc.273.36.23225. PMID 9722553.. ...
... and often such models are faster and cheaper to perform than biological assays. Such up-to-date computational models include ... Molecular break light assay for DNA adenine methyltransferase activity - an assay that relies on the specificity of the ... Bhasin, et al.,[102] Bock, et al.,[103] and Zheng, et al.[104] [105] Together with biological assay, these methods greatly ... the assay is similar in concept to the HELP assay.. *Methylated DNA immunoprecipitation (MeDIP), analogous to chromatin ...
M. F. Manzoli-Palma; N. Gobbi; M. S. Palma (2003). "Insects as biological models to assay spider and scorpion venom toxicity". ... Barbaro KC, Ferreira ML, Cardoso DF, Eickstedt VR, Mota I (November 1996). "Identification and neutralization of biological ...
Here she developed nanoparticle sensors for biological assays. In particular, Thanh used gold nanoparticles combined with ...
His doctorate thesis was a study of biological assays of cortical hormone and their application. His PhD supervisor was Dr ... "Biological assays of cortical hormone and their application". hdl:1842/17238. Cite journal requires ,journal= (help) "Archived ...
Genotyping is the process of elucidating the genotype of an individual with a biological assay. Also known as a genotypic assay ...
Together with biological assay, these methods greatly facilitate the DNA methylation analysis. 5-Hydroxymethylcytosine 5- ... Molecular break light assay for DNA adenine methyltransferase activity - an assay that relies on the specificity of the ... and often such models are faster and cheaper to perform than biological assays. Such up-to-date computational models include ... the assay is similar in concept to the HELP assay. Methylated DNA immunoprecipitation (MeDIP), analogous to chromatin ...
JSTOR 2983630 Fieller, EC (1944). "A fundamental formula in the statistics of biological assay, and some applications". ... 1940) "The biological standardisation of insulin". Journal of the Royal Statistical Society (Supplement). 1:1-54. ...
ATCC also serves to set standards for biological reagent and assay quality. These standards are used by the U.S. Food and Drug ... ATCC also provides biological repository management services to institutions, agencies and companies wishing to outsource the ... ATCC, along with the Centers for Disease Control, sold or sent biological samples of anthrax, West Nile virus and botulism to ... A number of these materials were used for Iraq's biological weapons research program, while others were used for vaccine ...
Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (2010). "Protein-binding assays in biological liquids using microscale ... Small molecules may be used as research tools to probe biological function as well as leads in the development of new ... Small molecules can have a variety of biological functions or applications, serving as cell signaling molecules, drugs in ... Pharmacology usually restricts the term "small molecule" to molecules that bind specific biological macromolecules and act as ...
"Protein-binding assays in biological liquids using microscale thermophoresis". Nature Communications. 1 (7): 100. Bibcode: ... Ligand efficacy refers to the ability of the ligand to produce a biological response upon binding to the target receptor and ... In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose ... ISBN 978-0-12-370599-0. Cabanos, C; Wang, M; Han, X; Hansen, SB (8 August 2017). "A Soluble Fluorescent Binding Assay Reveals ...
"Protein-binding assays in biological liquids using microscale thermophoresis". Nature Communications. 1 (7): 100. Bibcode: ... A single assay is typically completed in minutes and only requires a sample consumption of a few µl. Fluorescence polarization/ ... Recovery of Biological Products XIII. Quebec City, Quebec. Archived from the original on 2010-11-04. Attri AK, Minton AP ( ... Proximity ligation assay (PLA) in situ is an immunohistochemical method utilizing so called PLA probes for detection of ...
Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (October 2010). "Protein-binding assays in biological liquids using ... For example, microscale thermophoresis can be used to quantify how the biological matrix/liquid affects the affinity of a drug ... Chemical techniques are employed to measure the concentration of drugs in biological matrix, most often plasma. Proper ... the characteristics of the appropriate biological membranes and the way that substances can cross them, or the characteristics ...
Oct 2010). "Protein-binding assays in biological liquids using microscale thermophoresis". Nature Communications. 1 (7): 100. ...
May 2002). "Hemorrhagic fever viruses as biological weapons: medical and public health management". Journal of the American ... and detecting proteins by enzyme-linked immunosorbent assay (ELISA) are methods best used in the early stages of the disease ... It has the potential to be weaponised for use in biological warfare,[226][227] and was investigated by Biopreparat for such use ...
"The Journal of Biological Chemistry. 288 (7): 4878-90. doi:10.1074/jbc.M112.422410. PMC 3576092 . PMID 23275336.. ... When assayed in the human, the observed variability of the SP concentrations are large, and in some cases the assay methodology ... Unique among biological processes, SP release (and expression of its NK1 Receptor (through autocrine, paracrine, and endocrine- ... The Journal of Biological Chemistry. 279 (44): 45519-27. doi:10.1074/jbc.M408523200. PMID 15319441.. ...
Biomechanics is the study of the structure and function of biological systems by means of the methods of Mechanics. ... assays, and procedures needed for providing the specific services. Subspecialties include transfusion medicine, cellular ... Biophysics is an interdisciplinary science that uses the methods of physics and physical chemistry to study biological systems. ... Histology is the study of the structures of biological tissues by light microscopy, electron microscopy and ...
Sen, Pranab Kumar (December 1963). "On the estimation of relative potency in dilution(-direct) assays by distribution-free ... Biological Reviews of the Cambridge Philosophical Society. 82 (4): 591-605. doi:10.1111/j.1469-185X.2007.00027.x. PMID 17944619 ...
"Comparison of the Hershberger assay and androgen receptor binding assay of twelve chemicals". Toxicology. 195 (2-3): 177-86. ... from mediating their biological effects in the body. They act by blocking the androgen receptor (AR) and/or inhibiting or ... thereby preventing them from activating the receptor and mediating their biological effects.[57][58] AR antagonists are ...
ELISA antigen assays, plaque neutralization assays, and immunofluorescence essays. However, immunofluorescence essays provide ... Werner, Dietrich, editor (2004). Biological Resources and Migration. Springer. p. 363. ISBN 978-3-540-21470-0.. CS1 maint: ...
Biological roleEdit. Knockout mice of the PR have been found to have severely impaired lobuloalveolar development of the ... Biochemical assays showed that the +331G/A polymorphism increases transcription of the PR gene, favoring production of hPR-B in ... Biological process. • regulation of transcription, DNA-templated. • cell-cell signaling. • negative regulation of gene ... In vitro phosphorylation by casein kinase II". The Journal of Biological Chemistry. 269 (49): 31034-40. PMID 7983041.. ...
Other detection methods include the use of enzyme-linked-immuno-sorbent-assays and radial immunodiffusion. Different analytical ... Coakley RJ, Taggart C, O'Neill S, McElvaney NG (January 2001). "Alpha1-antitrypsin deficiency: biological answers to clinical ... The Journal of Biological Chemistry. 275 (35): 27258-65. doi:10.1074/jbc.M004850200. PMID 10867014. Kolarich D, Weber A, ...
Biological Sciences. 270 (1517): 819-26. doi:10.1098/rspb.2002.2290. PMC 1691313. PMID 12737660.. ... There are genetic assays being scheduled for lions to determine their genetic diversity. The preliminary studies show results ... The chances of such disorders are increased when the biological parents are more closely related. This is because such pairings ... Department of Anthropology and School of Biological & Biomedical Sciences, University of Durham, U.K.. ...
Separate but compatible signal amplification systems enable the multiplex assay (up to two targets per assay). Signal ... Dill, K.; Hui Liu, R.; Grodzinski, P. (2008). Microarrays: Preparation, Microfluidics, Detection Methods, and Biological ... Some assays are designed so that the secondary color will be present or absent in cases of interest. An example is the ... Single-molecule RNA FISH assays can be performed in simplex or multiplex, and can be used as a follow-up experiment to ...
... binding assay and pharmacological evaluation of 1,2,4-triazolo[1,5-a]pyrimidinone and 3-amino-1,2,4-triazole derivatives". ... function because the flexible side chains in these analogues do not have the conformations required for high biological ...
Endicott, S. L.; Hageman, E. (1998). The United States and Biological Warfare: Secrets from the Early Cold War and Korea. ... Serologically, an enzyme-linked immunosorbent assay during the acute phase of the disease using specific IgM against yellow ... Yellow fever has been researched by several countries as a potential biological weapon.[105] ...
The plaque assay was developed using poliovirus; the discovery of viral replication in culture was also with poliovirus in 1949 ... J Gen Virol 85(Pt 5):1145-1151 Mingxiao M, Ming L, Jian C, Song Y, Shude W, Pengfei L (2011) Molecular and biological ...
An assay to detect the presence of (1,3)-β-D-glucan in blood is marketed as a means of identifying invasive or disseminated ... Differences in molecular weight, shape, and structure of β-glucans dictate the differences in biological activity.[6][7] ... Mennink-Kersten MA, Ruegebrink D, Verweij PE (2008). "Pseudomonas aeruginosa as a cause of 1,3-β-D-glucan assay reactivity". ... "Multicenter clinical evaluation of the (1→3)β-D-glucan assay as an aid to diagnosis of fungal infections in humans". Clin ...
Michael's parents gifted him one of Darwin's books on evolution and biological mysteries. The book described biological clocks ... A locomotor behavior monitor was used to assay behavioral activity. The team watched and recorded fly activity through the day ... 2006: Pittendrigh/Aschoff Award from the Society for Research on Biological Rhythms[2] ...
Hall used immunoprecipitation assays to show that CNK1, a scaffold protein-like target of Rho, interacts with two Rho-specific ... "Biochemical and biological properties of the human N-ras p21 protein". Molecular and Cellular Biology. 7 (1): 541-544. doi ... He used strains of E. Coli with mutated B-lactamase, an antibiotic resistance enzyme, and assayed their activity in the ...
... have been tailored for various chemical and biological applications where higher photostability, different spectral ... "Fluorescein Transport Assay to Assess Bulk Flow of Molecules Through the Hypocotyl in Arabidopsis thaliana". Bio-Protocol. 8 ...
T cells (T lymphocytes) - T lymphocyte proliferation assay - T lymphocytes - T suppressor cells - T4 cell - T4 cells (T-helper ... biological response modifiers (BRMs) - biopsy - biotechnology - blinded study - blips - blood-brain barrier - body fat ... genotypic assay - germinal centers - giardiasis - globulins - glycoprotein - gonorrhea - gp120 (gp120) - gp160 (gp160) - gp41 ( ... enzyme-linked immunosorbent assay (ELISA) - eosinophil - eosinophilic folliculitis - epidemic - epidemiological surveillance - ...
Qi, Y.X. (March 2009). "Nested PCR Assay for Detection of Corynespora Leaf Fall Disease Caused by Corynespora cassiicola". ... the fungus has been proposed for use as a bioherbicide and an agent of biological pest control. List of soybean diseases ...
A biomarker, or biological marker is a measurable indicator of some biological state or condition. Biomarkers are often ... one or more available assay platform for such biomarker with test-retest reliability and reproducibility assay that meet assay ... The widespread use of the term "biomarker" dates back to as early as 1980.[8] The term "biological marker" was introduced in ... The term biomarker is also used to describe biological involvement in the generation of petroleum. ...
Alternative measurements include the Folin-Ciocalteu reagent, and the Trolox equivalent antioxidant capacity assay. The assay ... Oxygen radical absorbance capacity (ORAC) is a method of measuring antioxidant capacities in biological samples in vitro. A ... Huang D, Ou B, Prior RL (2005). "The chemistry behind antioxidant capacity assays". J. Agric. Food Chem. 53 (6): 1841-56. doi: ... Ou B, Hampsch-Woodill M, Prior RL (2001). "Development and validation of an improved oxygen radical absorbance capacity assay ...
Bentolila A. (2000)։ «Synthesis and heparin-like biological activity of amino acid-based polymers»։ Polymers for Advanced ... A quick anti-Xa-activity-based whole blood coagulation assay for monitoring unfractionated heparin during cardiopulmonary ...
Biological process. • apoptotic signaling pathway. • cellular response to mechanical stimulus. • response to lipopolysaccharide ... Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by an enzyme-linked immunosorbent assay". ... The Journal of Biological Chemistry. 272 (4): 2042-5. doi:10.1074/jbc.272.4.2042. PMID 8999898.. ... The Journal of Biological Chemistry. 271 (22): 12852-8. doi:10.1074/jbc.271.22.12852. PMID 8662842.. ...
Series B, Biological sciences. svezak 366 (broj 1579): str. 2782.-2789. PMC 3146779. PMID 21893541. doi:10.1098/rstb.2011.0097. ... Chen, J; Zhang, R, Wang, J, Liu, L, Zheng, Y, Shen, Y, Qi, T, Lu, H (2011). "Interferon-gamma release assays for the diagnosis ... Lawn, SD; Nicol, MP (2011). "Xpert® MTB/RIF assay: development, evaluation and implementation of a new rapid molecular ... U zemljama u razvoju se ne izvode interferon-γ release assays (IGRAs) niti tuberkulinski kožni test.[54][55] U osoba zaraženih ...
Biological Control 30:446-455. Kilic-Ekici, O., and G. Y. Yuen. 2003. Induced resistance as a mechanism of biological control ... These activities normally are phenotypically overwhelming and often lead to masking of other phenotypes in standard assays, ... Fusarium head blight biological control with Lysobacter enzymogenes. Biological Control 39:336-344. Giesler, L. J., and G. Y. ... Bean rust biological control using bacterial agents. Crop Protection 20:395-402. Jochum, C. C., L. E. Osborne, and G. Y. Yuen. ...
"The Journal of Biological Chemistry. 272 (46): 29083-90. doi:10.1074/jbc.272.46.29083. PMID 9360983.. ... "Search ChIP-qPCR Assay". sabiosciences. Archived from the original on 18 April 2015. Retrieved 17 April 2015.. ... "Biological Psychiatry. 77 (1): 43-51. doi:10.1016/j.biopsych.2014.05.006. PMC 4234692. PMID 24951455.. ... Biological process. • cell adhesion. • positive regulation of cell migration. • positive regulation of protein tyrosine kinase ...
Assays revealed that the mutated ERα showed strongly reduced transcriptional activity in response to stimulation by estradiol, ... that results in an inability of estrogen to mediate its biological effects in the body.[3] Congenital estrogen deficiency can ... An in vitro assay of ERα-dependent gene transcription found that the EC50 for transactivation had been reduced by 240-fold ...
Series B, Biological Sciences. 356 (1416): 1861-70. doi:10.1098/rstb.2001.0999. PMC 1088562. PMID 11779385.. ... One is called a Rapid Molecular Assay, when an upper respiratory tract specimen (mucus) is taken using a nasal swab or a ... Series B, Biological Sciences. 356 (1416): 1829-39. doi:10.1098/rstb.2001.1020. PMC 1088558. PMID 11779381.. ... Pinto LH, Lamb RA (April 2006). "The M2 proton channels of influenza A and B viruses". The Journal of Biological Chemistry. 281 ...
Biological Assays Topics. *Analytical Challenges with Potency Assays and Comparability for Cell & Gene Therapy Products ... Biological Assays 2021 Event Format. We are pleased to announce that this years Well Characterized Biologics & Biological ... "The Well Characterized Biologics and Biological Assays conference has a long history of attracting scientists and regulatory ... "The Well Characterized Biologics and Biological Assays conference has a long history of attracting scientists and regulatory ...
Biological Assay. Br Med J 1949; 2 doi: https://doi.org/10.1136/bmj.2.4629.688-b (Published 24 September 1949) Cite this as: Br ...
Biological Preparation to Assess Folate in Whole Blood and Serum Folate Microbiologic Assay Materials. ... Biological Preparation to Assess Folate in Whole Blood and Serum Folate Microbiologic Assay Materials ... CDC offers assay materials to laboratories measuring serum or whole blood folate by microbiologic assay. ... The WHO recommends the microbiologic assay for assessing folate status in population surveys (WHO, 2015) and this assay is ...
Isozyme and a flurophore was used to examine glycine in numerous biological samples. ... A high throughput enzymatic assay using improved Glycine Oxidase (GO) ... 2020, March 23). Detecting Glycine in Biological Samples Using Enzymatic Assays. News-Medical. Retrieved on March 07, 2021 from ... The Glycine Assay Kit offered by BioVision is a high-throughput screening assay designed for measuring the concentration of ...
We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record ... We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record ... Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity. ... Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity. ...
Finally, there was an update on data coordination, collaboration, and data harmonization to advance biological and therapeutics ... or similar reprogrammed cell assays. Participants represented the National Cooperative Reprogrammed Cell Research Groups ( ... along with current progress and need for improved cell assay technologies. ...
... design and use of hyperpolarized NMR probes in biological assays, and describe current limitations and developments. ... derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular ... The hyperpolarized probes are produced ex situ, introduced into biological systems and detected with high sensitivity and ... Hyperpolarized molecular probes for biological NMR spectroscopy enable the unbiased detection of complex processes by virtue of ...
A cryogenic detector is used for detection in a biological assay. ... A biological assay of this invention typically involves ... DETECTION OF THE ENERGY OF PHOTONS FROM BIOLOGICAL ASSAYS. The present invention relates to the detection of the energy of ... According to the present invention, we employ a cryogenic detector in a biological assay. A cryogenic detector operates at a ... Such a detector can detect the energy of photons from biological assays, notably those giving rise to changes in fluorescence. ...
SGS is a world leader in conducting potency assays. Our consultants can advise you on the most suitable tests for your product ... Life Sciences Potency Assays and Biological Activity Assays. As a regulatory requirement for the release of biological products ... Our assays, conducted by experts in their field, measure the efficacy or effectiveness of biological drug candidates in an in ... biological potency assays are of vital importance in the product development and manufacturing processes. ...
... Hybridoma. 1999 Dec;18(6):505-11. doi: ...
BIOLOGICAL ASSAY OF THYROID ACTIVITY Message Subject (Your Name) has forwarded a page to you from Journal of Pharmacology and ...
... and store assay endpoint data - ideal for assay development functions as well as LTS and HTS screening ... Arxspan Assay is a cloud-based software tool set that can define, collect, manage, ... Centrally house your biological assay data. Designed for use in studies which are centrally managed and where biological data ... Employ Arxspan Assay to provide study coordinators with an integrated view of study activity and assay data as reported by ...
A Highly Sensitive Chemiluminometric Assay for Real-Time Detection of Biological Hydrogen Peroxide Formation.. Zhu H1, Jia Z2, ... While a number of assays have been developed over the past decades for detecting and/or quantifying biological H2O2formation, ... The article discusses the usefulness and shortcomings of this chemiluminometric assay in detecting biological H2O2 formation ... A Highly Sensitive Chemiluminometric Assay for Real-Time Detection of Biological Hydrogen Peroxide Formation ...
... aims to present and discuss different tools available for the study of lipids and their main applications in biological assays. ... Lipids are heterogeneous biological molecules with many important roles. In human body, lipids can be energy substrates, ... most appropriate one for the analysis as to answer a scientific question about lipid composition and role within a biological ... Mass spectrometry: application in biological assays. In biological assays, lipidomics-MS analysis is highly applied to generate ...
Optimal detection of cholinesterase activity in biological samples: modifications to the standard Ellmans assay.. [Dominika ... Ellmans assay is the most commonly used method to measure cholinesterase activity. It is cheap, fast, and reliable, but it has ... To eliminate possible interference of DTNB with enzyme hydrolysis, we introduce a modification of the standard Ellmans assay. ... Using enzyme-linked immunosorbent assay (ELISA) to enrich tissue homogenates for cholinesterase while depleting the sample of ...
The sample may be a biological fluid such as an oral fluid. ... A method for performing time resolved homogeneous assays using ... In particular, the invention relates to a corrected method for biological homogeneous assays.. 2. Description of Related Art. ... 1. A method for performing a homogeneous biological assay comprising: preparing at least one reaction volume and a matrix ... have the potential to impact assay results. With a heterogeneous assay, this is not usually a concern when the sample result is ...
Biological assays explanation free. What is Biological assays? Meaning of Biological assays medical term. What does Biological ... Looking for online definition of Biological assays in the Medical Dictionary? ... redirected from Biological assays). Also found in: Dictionary, Thesaurus, Encyclopedia.. Related to Biological assays: bioassay ... The database currently tags 158,000 individuals working with Biological Assays.. Maven Semantic: Biological Assay Research ...
Assay, New Type Of Biological Food Anti - Oxygen And Antiseptic D-Sodium Erythorbate D-sodium erythorbate, It is a new type of ... C6h7nao6 Biological Antimicrobial Food Preservatives 98 0 100 5% Assay - China. price: check with company for price. C6h7nao6 ... New Type Of Biological Food Anti - Oxygen And Antiseptic D-Sodium Erythorbate D-sodium erythorbate, It is a new type of ... High Assay Antimicrobial Food Additives Sorbic Acid With 132 135 Melting Range Cereal and pulse products - XIAMEN ASAU IMPORT ...
... ... Addressing the obstacle of complex formation of UST with nADA, we developed an acidification assay to approach the total amount ... Nevertheless, the immune system can recognize the administered biological as non-self and respond with generation of anti-drug ... Validated methods were based on surface plasmon resonance spectroscopy (SPR), enzyme-linked immunosorbent assay (ELISA) and a ...
Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with ...
A biological fluid assay system and method for the determination of immunoreactive characteristics of biological specimen and ... US4837160A - Biological fluid assay system and method - Google Patents. Biological fluid assay system and method Download PDF ... A biological fluid assay system and method for the determination of immunoreactive characteristics of biological specimen and ... Biological fluid assay system and method Related Parent Applications (1). Application Number. Title. Priority Date. Filing Date ...
Assay Acceptance and Sample Acceptance. For plate-based biological potency assays, we propose two separate sets of acceptance ... For plate-based biological potency assays, we propose the following two separate sets of acceptance criteria: assay acceptance ... Assay Acceptance Criteria for Multiwell-Plate-Based Biological Potency Assays. View PDF ... biochemical assays (such as clotting-factor activity assays) considered as functional assays or as a separate class ...
Find out information about Biological assays. A method for the quantitation of the effects on a biological system by its ... exposure to a substance, as well as the quantitation of the concentration of a... Explanation of Biological assays ... redirected from Biological assays). Also found in: Dictionary, Thesaurus, Medical.. Related to Biological assays: bioassay ... Biological assays , Article about Biological assays by The Free Dictionary https://encyclopedia2.thefreedictionary.com/ ...
... Gaëtan Chicanne,* Sonia Severin,* ... The assay was found to be linear with pure PtdIns3P quantities varying from a few pmol up to 100 pmol (Figure 2A). To assay the ... In the present study, we describe a mass assay to quantify this lipid from various biological samples using the recombinant ... In the present study, we propose a convenient mass assay to accurately quantify PtdIns3P from various biological samples, ...
... dc.creator. ... A Comparison Between the Spectrophotometric and Biological Assay for the Vitamin A Content of Fish-Liver Oils. en_US. ...
Harvey, A.L. (2009) Biological screening assays for plant secondary metabolites. Integrative and Comparative Biology, 49 (1). ...
This review discusses general aspects on miniaturization of biological assays and in particular summarizes single-cell assays ... Miniaturization of biological assays: Overview on microwell devices for single-cell analyses. Lindstrom, Sara KTH, School of ... Miniaturization, Microwell, Single cell, Well design, Well-based assay, In vitro cell assay National Category Biochemistry and ... General significance: In vitro assays offering low volume sampling and rapid analysis in a high-throughput manner are of great ...
SCIEN Colloquium presents Leveraging lanthanide-based spectral encoding for highly multiplexed biological assays ... We are now applying these MRBLEs towards a wide variety of biological problems, from high-throughput and quantitative profiling ... provides a particularly appealing encoding strategy because of the ease of reading codes and assay flexibility. We recently ... in physics for work with Professor Steve Block developing instrumentation and assays for single-molecule studies of kinesin ...
Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products ... Assay Specificity and Selectivity. The Assay specificity and sensitivity data was only determined for the AlphaLISA assay ... and intra-assay precision testing is used in conjunction with the above Assay Sensitivity data for determination of assay ... Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products Download Related Products: ...
  • In this article, BioVision describes an easy, simple, and high-throughput enzymatic assay using an enhanced Glycine Oxidase (GO) Isozyme and a fluorophore in a 96-well format. (news-medical.net)
  • General significance: In vitro assays offering low volume sampling and rapid analysis in a high-throughput manner are of great interest in a wide range of single-cell applications. (diva-portal.org)
  • Encoded microparticles have become a powerful tool for a wide array of applications, including high-throughput sample tracking and massively parallel biological multiplexing. (stanford.edu)
  • We are now applying these MRBLEs towards a wide variety of biological problems, from high-throughput and quantitative profiling of protein-peptide interactions to specific and sensitive detection of multiple bacterial species from blood for fast diagnosis of sepsis. (stanford.edu)
  • For efficient analysis and characterization of biological ice nuclei under immersion freezing conditions, we developed the Twin-plate Ice Nucleation Assay (TINA) for high-throughput droplet freezing experiments, in which the temperature profile and freezing of each droplet is tracked by an infrared detector. (atmos-meas-tech.net)
  • The results confirm that TINA is suitable for high-throughput experiments and efficient analysis of biological IN in laboratory and field samples. (atmos-meas-tech.net)
  • Hence, in this study, we have developed a high-throughput assay for screening and indentifying natural products that can enhance NK cell-mediated killing of cancer cells. (biomedcentral.com)
  • Our results demonstrated that this IFN-γ based high-throughput assay for screening of natural products for NK cell tumoricidal activity is a simple, economic and reliable method. (biomedcentral.com)
  • However, lack of simple and economic approach for large scale screening of the natural products has been a big impediment and thus warrants a development of a novel high-throughput assay for screening of novel and efficient small molecules from a pool of thousands of natural products. (biomedcentral.com)
  • We propose a new combined method that uses chemiluminescent antibody detection of the residual release strand to give a robust helicase assay, that does not employ radiolabelled compounds and gives a stable read-out that is well suited to high-throughput screening. (scienceexchange.com)
  • Using in vitro cytotoxicity assays provides a high-throughput method of screening compounds to determine selectivity of toxicity in fish cells. (viterbo.edu)
  • Algorithms after the cellular download hormone assays in biological fluids of Iraq explained a relative intervention availability, Baghdad were checkout and familiarity principles to shore withdrawn sources in and around Kirkuk in the organized devices. (pretaparty.com)
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  • It is used that the SUs get in download Hormone Assays in Biological Fluids 2013 Equivalent and selected code on layout of instrumentation covers supported to browse the private gloire growth listeriolysin. (ligaya-technologies.com)
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  • Using enzyme-linked immunosorbent assay (ELISA) to enrich tissue homogenates for cholinesterase while depleting the sample of sulfhydryl groups eliminates unwanted interactions with DTNB, making it possible to measure low cholinesterase activity in biological samples. (sigmaaldrich.com)
  • Validated methods were based on surface plasmon resonance spectroscopy (SPR), enzyme-linked immunosorbent assay (ELISA) and a cell-based approach to characterize neutralizing capacity of nADA. (fraunhofer.de)
  • Here we describe a new capture enzyme-linked immunosorbent assay (cELISA) based on monoclonal antibodies specific for lipopolysaccharide (LPS) of Francisella tularensis subsp. (asm.org)
  • extracellular matrix assays for use with mammalian cells, tissues and fluids. (biocolor.co.uk)
  • The method is employed to quantify the catalytic activity of phospholipase A2 (PLA2) in both pure and complex biological fluids. (chalmers.se)
  • This chapter discusses a sensitive radioreceptor assay for lα,25-dihydroxyvitamin D in biological fluids. (elsevier.com)
  • Some studies describe 1α,25-dihydroxyvitamin D [lα,25-(OH) 2 D] as the hormonal metabolite responsible for mediating intestinal transport of these minerals and hence necessitate development of a precise method for quantitating this sterol in biological fluids, particularly serum. (elsevier.com)
  • 2018). CDC offers assay materials containing common key reagents to laboratories conducting the folate microbiologic assay for epidemiologic use. (cdc.gov)
  • Inter variability: Glycine Standard Curve was assayed in triplicate using three sets of reagents. (news-medical.net)
  • These two assays are the first in its virology reagents portfolio. (laraza.com)
  • Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury. (biotek.tw)
  • The assay provides researchers the ability to examine a large number of samples and offers an innovative tool in the area of neuroscience research. (news-medical.net)
  • Glycine concentration was evaluated using BioVision's Glycine Assay Kit in human serum samples from patients known to have Neurological conditions. (news-medical.net)
  • Serum Samples were serially diluted using Glycine Assay Buffer (Dilution Factor Range: 4-128). (news-medical.net)
  • More particularly but not exclusively, the present invention relates to the detection of fluorescence, and especially the detection of fluorescence- labelled biological samples. (google.co.uk)
  • Optimal detection of cholinesterase activity in biological samples: modifications to the standard Ellman's assay. (sigmaaldrich.com)
  • It is cheap, fast, and reliable, but it has limitations when used for biological samples. (sigmaaldrich.com)
  • Thus, assay design - especially the positioning of individual samples within and between plates - is crucial to obtaining valid results and thus needs to be considered when setting acceptance criteria for an assay. (bioprocessintl.com)
  • This paper is restricted to discussion of tests applied to the responses of a bioassay system to reference standards, controls and test samples obtained during performance of a potency assay. (bioprocessintl.com)
  • In the present study, we describe a mass assay to quantify this lipid from various biological samples using the recombinant PtdIns3 P 5-kinase, PIKfyve. (pubmedcentralcanada.ca)
  • Methods are described for application of the assay techniques to other types of biological and food samples. (nih.gov)
  • Additionally, the Luminex assay works in a multiplex format, simultaneously detecting four category A and B bacterial bioterrorism agents and is suitable for detecting Brucella in complex samples. (biomedcentral.com)
  • This particular chapter provides a detailed description of the steps to take when performing a population assay - from sample size (at least four test samples) to creating a dilution series, heat shock time and temps, and incubation time and temps. (mesalabs.com)
  • Making use of a protein found in animals which has evolved to selectively bind metabolites, an assay was developed which better classified samples compared to current methods used in the field of metabolomics. (montana.edu)
  • Hyperpolarized molecular probes for biological NMR spectroscopy enable the unbiased detection of complex processes by virtue of the high spectral resolution, structural specificity and quantifiability of NMR signals. (mdpi.com)
  • A cryogenic detector is used for detection in a biological assay. (google.co.uk)
  • 1. The use of a cryogenic detector for detection of photons in a biological assay. (google.co.uk)
  • The present invention relates to the detection of the energy of photons from biological assays, including those giving rise to characterisitic transmission, reflectance and absorbance spectra. (google.co.uk)
  • A Highly Sensitive Chemiluminometric Assay for Real-Time Detection of Biological Hydrogen Peroxide Formation. (nih.gov)
  • Detection and measurement of the levels or rates of production of cellular H 2 O 2 are instrumental in studying the biological effects of this major ROS. (nih.gov)
  • This article describes a highly sensitive assay for real-time detection of H 2 O 2 formation in cultured cells and isolated mitochondria. (nih.gov)
  • Inclusion of exogenous catalase in the assay, i.e., detection of catalase-inhibitable luminol/HRP-dependent CL, makes it possible to specifically determine the flux of H 2 O 2 in the extracellular milieu. (nih.gov)
  • The detection limit of the assay was 10 3 to 10 4 bacteria/ml. (asm.org)
  • To complement molecular detection systems for potential bioterror agents, as required by international biodefense regulations, sets of mAbs were generated by B cell hybridoma technology and used to develop immunological assays. (biomedcentral.com)
  • The Luminex assay recognised all tested Brucella species with 'smooth' LPS with detection limits of 2 × 10 2 to 8 × 10 4 cells per mL, depending on the species tested. (biomedcentral.com)
  • Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. (biotek.tw)
  • abstract = "The structure, stability, and aggregation potential of short Au nanorods under biological-based solution conditions have been studied. (elsevier.com)
  • Development of Asian carp liver cell lines for biological assays Justine Nelson (Tammy Clark) Abstract The Asian carp are an invasive species that have a high probability of causing ecological and economic effects to the fishing industry where inhabitants are found. (viterbo.edu)
  • We are pleased to announce that this year's Well Characterized Biologics & Biological Assays event will be free-to-attend and delivered in a 100% digital format, held October 25-28, 2021. (informaconnect.com)
  • This conference offers an excellent opportunity for participants to learn from the experiences of regulators, industry leaders and experienced scientists, including statisticians, who have played a part in the successful development of a wide array of well-characterized biologics and biological assays, and to engage in open discussions about the current challenges faced by numerous stakeholders. (informaconnect.com)
  • The Well Characterized Biologics and Biological Assays conference has a long history of attracting scientists and regulatory professionals with a profound interest in developing best practices for analytical strategies. (informaconnect.com)
  • Well Characterized Biologics & Biological Assays is the only event in the industry that provides the opportunity to learn from and interact directly with the regulators who will review and approve your CMC programs. (knect365.com)
  • Although there exists a current demand for higher sensitivity, the method of C-peptide quantitative analysis by Isotope-dilution assays allows for greater specificity as compared to current immunoassay methods. (umsystem.edu)
  • Biological responses were more highly correlated with Cd concentrations evaluated using bioavailable methods than with total concentrations. (lancs.ac.uk)
  • In contrast to common physical or chemical methods, a bioassay results in detailed information on the biological activity of a substance. (bioassay.de)
  • All methods are suitable for analysing the results obtained by several biological assays. (bioassay.de)
  • Other assay methods for this type of enzyme analyse ATPase activity but assessment of the effects on the helicase unwinding activity is considered the method of choice forevaluating inhibitors of this class of enzyme (1). (scienceexchange.com)
  • Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points. (biotek.tw)
  • There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models. (biotek.tw)
  • Moreover, unlike enzymatic assays (e g, elastase), there is no confusion as to whether the putative scavenger actually reacts with HOCl or inhibits the enzyme. (istanbulc.edu.tr)
  • Many parallel biochemical assays rely on thin aqueous films to spread a reactant solution over a wide area decorated with multiple distinct substrates. (cornell.edu)
  • In vitro assays with rat mesenchymal stem cells (rMSCs) and rat endothelial progenitor cells (rEPCs) also showed that rMSCs attached and proliferated on the materials influenced by the calcium content in the environment. (rsc.org)
  • With the rapid development of technologies that have application to both the design of novel biological products and alternative bioassays for product characterization, new technical and regulatory issues will continue to emerge. (informaconnect.com)
  • The general approach of most bioassays is to perform a dilution assay, which measures the biological responses at several doses. (bioassay.de)
  • The ligand affinity and specificity of this cytosolic receptor has been exploited to develop a sensitive competitive protein binding assay for lα,25-(OH) 2 D. This radioreceptor assay has been instrumental in the phosphate-related disease states, as well as in characterizing the physiological regulation of lα,25-(OH) 2 D. Specific components for the radioreceptor assay are described in the chapter. (elsevier.com)
  • Chromagen will develop biological assays and screen CombiChem compounds, while receiving research support and a percentage of future payments received by CombiChem upon outlicensing of drug candidates. (thefreedictionary.com)
  • The characterized compounds were screened to identify if they interfere with mammalian type I DNA topoisomerase activity via in vitro supercoil relaxation assays. (aacrjournals.org)
  • Selected compounds were subjected to cytostatic assays using HeLa (cervix adenocarcinoma), MCF7 (breast adenocarcinoma) and A431 (skin epidermoid carcinoma) cells. (aacrjournals.org)
  • To the best of our knowledge no biological studies and syntheses of these compounds have been reported. (hindawi.com)
  • Zhang M, Sternberg MR, Pfeiffer CM. Harmonizing the calibrator and microorganism used in the folate microbiological assay increases the comparability of serum and whole blood folate results in a CDC round-robin study. (cdc.gov)
  • analytical test method qualification and validation, as well as pre- and post- approval product comparability studies, and assay bridging/technology transfers. (informaconnect.com)
  • Deproteinized homogenates were serially diluted using Glycine Assay Buffer (Dilution Factor: 4-16). (news-medical.net)
  • Analytic dilution assays using multiwell plates constitute the most common platform for measurement of biological activity by in vitro bioassay or measurement of immunoreactivity by immunoassay. (bioprocessintl.com)
  • A key assumption of a dilution assay is that the active component follows the same principle of activity in standard and in sample preparation. (bioassay.de)
  • The term dilution assay is generally used to designate a special type of bioassay in which one or more preparations (e.g. a drug) are administered to experimental units at different dose levels inducing a measurable biological response. (wikipedia.org)
  • Dilution assays can be direct or indirect. (wikipedia.org)
  • In a direct dilution assay the amount of dose needed to produce a specific (fixed) response is measured, so that the dose is a stochastic variable defining the tolerance distribution. (wikipedia.org)
  • Conversely, in an indirect dilution assay the dose levels are administered at fixed dose levels, so that the response is a stochastic variable. (wikipedia.org)
  • For a mathematical definition of a dilution assay an observation space U {\displaystyle U} is defined and a function f : U → R {\displaystyle f:U\rightarrow \mathbb {R} } so that the responses u ∈ U {\displaystyle u\in U} are mapped to the set of real numbers. (wikipedia.org)
  • Of notable importance in this respect is the theory of Generalized Linear Models with which a wide range of dilution assays can be modelled. (wikipedia.org)
  • Nevertheless, the immune system can recognize the administered biological as non-self and respond with generation of anti-drug antibodies (ADA), including neutralizing ADA (nADA). (fraunhofer.de)
  • To this end, monoclonal antibodies (mAbs) specific for Brucella LPS were generated and used to design a highly specific and sensitive antigen capture assay. (biomedcentral.com)
  • Additionally, we have developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. (frontiersin.org)
  • Additionally, slope-ratio and dichotomous assays (quantal response assays) are available as well. (bioassay.de)
  • Incubation (37 degrees C for at least 30 min) of the buffer to which AAPH was dissolved was critical in decreasing assay variability. (nih.gov)
  • For standard assays, mix 75 μl of 1 M phosphate buffer (PBS) pH 7.0, containing 1 M NaCl with 2.5 ng of the partially annealed DNA duplex for each well. (scienceexchange.com)
  • They also discussed measures needed to assess iPSC-derived cell-type fidelity and physiological relevance to their in vivo counterparts, along with current progress and need for improved cell assay technologies. (nih.gov)
  • In preclinical cancer research, bioluminescence imaging with firefly luciferase and D -luciferin has become a standard to monitor biological processes both in vitro and in vivo . (nature.com)
  • In vivo assays showed that hybrid composite PLA-ORMOGLASS fibers were able to promote the formation of blood vessels. (rsc.org)
  • The combination of mAbs most suitable for an antigen capture assay format was identified and an immunoassay using the Luminex xMAP technology was developed. (biomedcentral.com)
  • A history of biological standardization : the characterization and measurement of complex molecules important in clinical and research medicine : contributions from the UK, 1900-1995 : what, why, how, where, and by whom / a personal account by D.R. Bangham. (who.int)
  • As a regulatory requirement for the release of biological products onto the market, biological potency assays are of vital importance in the product development and manufacturing processes. (sgs.com)
  • Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)
  • Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. (biotek.tw)
  • The sample may be a biological fluid such as an oral fluid. (patentgenius.com)
  • 2. The method of claim 1 wherein said biological fluid is an oral fluid. (patentgenius.com)
  • A device for collecting and analyzing a biological fluid is provided. (patentsencyclopedia.com)
  • More particularly, this invention relates to a device for collecting and analyzing a biological fluid. (patentsencyclopedia.com)
  • Single lipid vesicle assay for characterizing single-enzyme kinetics of phospholipid hydrolysis in a complex biological fluid. (chalmers.se)
  • This work sets out to investigate biological responses of Eisenia fetida to Cd, based on the bioavailable rather than total concentration of Cd, in soils. (lancs.ac.uk)
  • Antioxidant system responses in E. fetida to Cd were measured as biological endpoints. (lancs.ac.uk)
  • Cd concentration measured using DGT and CaCl2 extraction provided the narrowest ranges of lowest observed effect concentration (LOEC) values based on biological responses, indicating the potential use of these measurements in management of soil quality and setting soil remediation standards. (lancs.ac.uk)
  • Inter variability: Glycine Standard Curve was assayed in triplicate according to the protocols using the same components. (news-medical.net)
  • Multiple components of the overall assay system - from instruments to incubation media - need to be within defined limits to permit execution of a valid assay, so they are tested for suitability either before or during the assay. (bioprocessintl.com)
  • The biological material in which the effect is measured can range from subcellular components and microorganisms to groups of animals. (thefreedictionary.com)
  • Lack of biological assays to measure many of these contaminants in tissue specimens, the high cost of using these assays in large epidemiologic studies when they were available, and challenges related to the interpretation of the results when exposures were measured after the adverse reproductive event occurred limit the conclusions that can be drawn from these investigations. (thefreedictionary.com)
  • Thus, conventional problems of the TNB assay arising from the reactivity of thiol-type scavengers to produce extra TNB by direct reduction of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) were overcome. (istanbulc.edu.tr)
  • Together with an improvement in the LOD of at least 1 order of magnitude compared to that of conventional assays, this suggests that the method will become useful in furthering our understanding of the role of PLA2 in health and disease and in detecting the pharmacodynamic effects of PLA2-targeting drug candidates. (chalmers.se)
  • Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. (biotek.tw)
  • Employ Arxspan Assay to provide study coordinators with an integrated view of study activity and assay data as reported by research team(s), and empower individual researchers to collaborate. (bruker.com)
  • Ellman's assay is the most commonly used method to measure cholinesterase activity. (sigmaaldrich.com)
  • 1. Determination of the strength or biological activity of a substance, such as a drug, by comparing its effects with those of a standard preparation on a test organism. (thefreedictionary.com)
  • Early estimates of CCK-like activity in blood were based on biological assays such as pancreatic secretion or gallbladder contraction. (thefreedictionary.com)
  • This study was designed to investigate impact of tannins on in vitro ruminal fermentation parameters as well as relationships between concentration and in vitro biological activity of tannins present in tree fruits. (scielo.org.za)
  • Correlation between in vitro biological activity and phenolic concentration was determined. (scielo.org.za)
  • Moreover, TINA was applied to investigate the influence of chemical processing on the activity of biological IN, in particular the effects of oxidation and nitration reactions. (atmos-meas-tech.net)
  • A rapid assay for the biological evaluation of helicase activity. (scienceexchange.com)
  • A new assay for the measurement of helicase enzyme activity was developed for the evaluation of the potency of potential inhibitors. (scienceexchange.com)
  • Helicase activity assays rely on the ability of the enzyme to displace one strand (release strand) of DNA or RNA from another (template) strand. (scienceexchange.com)
  • Precursor forms of IL-18 and IL-1β lack a signal peptide and they require cleavage by caspase-1 (IL-1β-converting enzyme) for their maturation, and hence for their biological activity ( 15 , 16 , 17 , 18 ). (jimmunol.org)
  • We also studied the morphology of treated and untreated larvae using scanning electron microscopy (SEM), and assessed the agonistic/antagonistic activity of SERMs in a human embryonic kidney cell line using a luciferase reporter assay system. (biomedcentral.com)
  • The NIMH Division of Neuroscience and Basic Behavioral Science convened a workshop of scientists involved in several ongoing NIMH-funded research efforts involving human induced pluripotent stem cells (iPSCs) or similar reprogrammed cell assays. (nih.gov)
  • CDC offers assay materials to laboratories measuring serum or whole blood folate by microbiologic assay. (cdc.gov)
  • Finally, there was an update on data coordination, collaboration, and data harmonization to advance biological and therapeutics discovery for mental illnesses. (nih.gov)
  • This assay, which is applicable to small molecule antioxidants and tissue homogenates, proved to be efficient for thiol-type antioxidants for which the widely used 5-thio-2-nitrobenzoic acid (TNB) test is not accurately responsive. (istanbulc.edu.tr)
  • The biological response may be quantal (e.g. positive/negative) or quantitative (e.g. growth). (wikipedia.org)
  • Perhaps there is no perfect assay for sensitively and accurately quantifying H 2 O 2 as well as other ROS in cells, wherein numerous potential reactants are present to interfere with the reliable measurement of the specific ROS. (nih.gov)
  • Since the measurement of cell viability was based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to formazan crystal by mitochondrial dehydrogenases [ 11 ], in the current study we revisited our previous results, checking for a possible interference of Rottlerin in the MTT assay. (biomedcentral.com)
  • These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. (mdpi.com)
  • A diverse and large set of data ranging from chemistry leads to biological assays and protein sequencing will now be managed and analysed through the Dotmatics Platform. (thefreedictionary.com)
  • As proof of concept, the HCV helicase was expressed and isolated using recombinant protein techniques and then used in our assay. (scienceexchange.com)
  • For D. cinerea fruits, colorimetric assays were poorly correlated to increases in gas production due to PEG treatment. (scielo.org.za)
  • Conditions for controlling temperature effects and decreasing assay variability using fluorescein as the fluorescent probe were validated in different laboratories. (nih.gov)
  • Our aim was to specifically detect Brucellae with 'smooth' lipopolysaccharide (LPS) using a highly sensitive monoclonal antibody (mAb) based immunological assay. (biomedcentral.com)
  • The aim of this study was to develop a rapid and sensitive immunological assay to detect all Brucellae with 'smooth' LPS, particularly B. melitensis and B. abortus . (biomedcentral.com)
  • bioassay ( biological assay ) A controlled experiment for the quantitative estimation of a substance by measuring its effect in a living organism. (encyclopedia.com)
  • I am looking forward to discussions of common challenges, strategies, and solutions for biological characterization and bioassay development. (informaconnect.com)
  • A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. (patentgenius.com)
  • A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a substance by some observable effect on a biological system. (thefreedictionary.com)
  • Statistical method in biological assay / David J. Finney. (who.int)
  • A fluorometric method for the estimation of the vitamin B1 content in food and biological materials is described. (semanticscholar.org)
  • The implementation of this method in the study of complex biological systems would have an immediate impact in academic and medical research. (montana.edu)
  • Our search for new antibiotics led to the syntheses and biological evaluation of new classes of dicarboxylic acid analogues. (hindawi.com)
  • WHO HQ Library catalog › Results of search for 'su:{Biological assay. (who.int)
  • BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility. (biotek.tw)
  • It includes information on all aspects of drug research and development, from drug discovery to biological assays , pharmacokinetics, clinical trials, and post-market surveillance. (thefreedictionary.com)
  • For efficient data analysis and clinical genotyping report generation, DeepChek® SingleRound assays are bundled with the ViroScore® and DeepChek® software, both CE-marked for IVD use, for Sanger and NGS, respectively. (laraza.com)
  • The hyperpolarized probes are produced ex situ , introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. (mdpi.com)
  • A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. (mdpi.com)
  • Here, we provide a survey of strategies used for the selection, design and use of hyperpolarized NMR probes in biological assays, and describe current limitations and developments. (mdpi.com)
  • Lerche, M.H. Hyperpolarized NMR Probes for Biological Assays. (mdpi.com)
  • An assay that uses a living system, such as an intact cell, to measure an effect or a molecule of interest. (thefreedictionary.com)
  • Much of the following discussion is based on cell-based assays in 96-well plates, which reflects their wide use in potency testing. (bioprocessintl.com)
  • Scope of review: This review discusses general aspects on miniaturization of biological assays and in particular summarizes single-cell assays in microwell formats. (diva-portal.org)
  • The course includes several aspects of assay development applicable to both target-based and cell-based assays, however, the focus will be on cell-based assays. (umu.se)
  • The assay carried out in the course includes cell culturing and bacterial infection, thus experience in cell culturing and good microbial lab practice is required. (umu.se)
  • Parameters of importance for optimization of a cell based assay will be tested empirically in the laboratory and their significance calculated using Z'. During the course a small-scale screen will be executed. (umu.se)
  • Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis. (biotek.tw)
  • Cell migration and invasion are live cell kinetic assays useful in oncology research. (biotek.tw)
  • The objective of this project was to create bighead and silver carp liver cell lines to be used in biological assays. (viterbo.edu)
  • 10 The suitable biological performance of these scaffolds was mainly attributed to their stiffness and the ions released from the glass, in particular calcium ions, as well as the intrinsic surface properties of the materials. (rsc.org)
  • The Canadian team of organic chemists and enzymologists developed drug design motifs, and pursued preparative chemistry, enzymology, molecular biology, and tissue culture studies that were interfaced with biological assays and pharmacokinetic studies carried out in Syntex, Palo Alto, CA. (thefreedictionary.com)