Biogenic Polyamines: Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA.PolyaminesSpermine: A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.Putrescine: A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.Spermidine: A polyamine formed from putrescine. It is found in almost all tissues in association with nucleic acids. It is found as a cation at all pH values, and is thought to help stabilize some membranes and nucleic acid structures. It is a precursor of spermine.Biogenic Amines: A group of naturally occurring amines derived by enzymatic decarboxylation of the natural amino acids. Many have powerful physiological effects (e.g., histamine, serotonin, epinephrine, tyramine). Those derived from aromatic amino acids, and also their synthetic analogs (e.g., amphetamine), are of use in pharmacology.Electrophoresis, Capillary: A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)Spectroscopy, Fourier Transform Infrared: A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.Receptors, Biogenic Amine: Cell surface proteins that bind biogenic amines with high affinity and regulate intracellular signals which influence the behavior of cells. Biogenic amine is a chemically imprecise term which, by convention, includes the catecholamines epinephrine, norepinephrine, and dopamine, the indoleamine serotonin, the imidazolamine histamine, and compounds closely related to each of these.Ornithine Decarboxylase: A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.Eflornithine: An inhibitor of ORNITHINE DECARBOXYLASE, the rate limiting enzyme of the polyamine biosynthetic pathway.Octopamine: An alpha-adrenergic sympathomimetic amine, biosynthesized from tyramine in the CNS and platelets and also in invertebrate nervous systems. It is used to treat hypotension and as a cardiotonic. The natural D(-) form is more potent than the L(+) form in producing cardiovascular adrenergic responses. It is also a neurotransmitter in some invertebrates.Cadaverine: A foul-smelling diamine formed by bacterial decarboxylation of lysine.Adenosylmethionine Decarboxylase: An enzyme that catalyzes the decarboxylation of S-adenosyl-L-methionine to yield 5'-deoxy-(5'-),3-aminopropyl-(1), methylsulfonium salt. It is one of the enzymes responsible for the synthesis of spermidine from putrescine. EC 4.1.1.50.Diamines: Organic chemicals which have two amino groups in an aliphatic chain.Biogenic Monoamines: Biogenic amines having only one amine moiety. Included in this group are all natural monoamines formed by the enzymatic decarboxylation of natural amino acids.Ornithine: An amino acid produced in the urea cycle by the splitting off of urea from arginine.Tyramine: An indirect sympathomimetic. Tyramine does not directly activate adrenergic receptors, but it can serve as a substrate for adrenergic uptake systems and monoamine oxidase so it prolongs the actions of adrenergic transmitters. It also provokes transmitter release from adrenergic terminals. Tyramine may be a neurotransmitter in some invertebrate nervous systems.Circular Dichroism: A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.G-Quadruplexes: Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)Polylysine: A peptide which is a homopolymer of lysine.Scopolamine Hydrobromide: An alkaloid from SOLANACEAE, especially DATURA and SCOPOLIA. Scopolamine and its quaternary derivatives act as antimuscarinics like ATROPINE, but may have more central nervous system effects. Among the many uses are as an anesthetic premedication, in URINARY INCONTINENCE, in MOTION SICKNESS, as an antispasmodic, and as a mydriatic and cycloplegic.EthylaminesKidney Papillary Necrosis: A complication of kidney diseases characterized by cell death involving KIDNEY PAPILLA in the KIDNEY MEDULLA. Damages to this area may hinder the kidney to concentrate urine resulting in POLYURIA. Sloughed off necrotic tissue may block KIDNEY PELVIS or URETER. Necrosis of multiple renal papillae can lead to KIDNEY FAILURE.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Glutamine: A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.Carboxymethylcellulose Sodium: A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.Camellia sinensis: Camellia sinensis L. (formerly Thea sinensis) is an evergreen Asiatic shrub of the THEACEAE family. The infusion of leaves of this plant is used as Oriental TEA which contains CAFFEINE; THEOPHYLLINE; and epigallocatechin gallate.Camellia: A plant genus in the family THEACEAE, order THEALES best known for CAMELLIA SINENSIS which is the source of Oriental TEA.Tea: The infusion of leaves of CAMELLIA SINENSIS (formerly Thea sinensis) as a beverage, the familiar Asian tea, which contains CATECHIN (especially epigallocatechin gallate) and CAFFEINE.Amines: A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed)Catechin: An antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.Starlings: The family Sturnidae, in the order PASSERIFORMES. The starling family also includes mynahs and oxpeckers.Gingival NeoplasmsMites: Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.Goblet Cells: A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.Ki-67 Antigen: A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.Genes, myc: Family of retrovirus-associated DNA sequences (myc) originally isolated from an avian myelocytomatosis virus. The proto-oncogene myc (c-myc) codes for a nuclear protein which is involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Truncation of the first exon, which appears to regulate c-myc expression, is crucial for tumorigenicity. The human c-myc gene is located at 8q24 on the long arm of chromosome 8.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.o-Phthalaldehyde: A reagent that forms fluorescent conjugation products with primary amines. It is used for the detection of many biogenic amines, peptides, and proteins in nanogram quantities in body fluids.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Octanes: Eight-carbon saturated hydrocarbon group of the methane series. Include isomers and derivatives.Metabolomics: The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.History, 20th Century: Time period from 1901 through 2000 of the common era.BooksNitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Peer Review: An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.Leucine Transaminase: A PYRIDOXAL PHOSPHATE containing enzyme that catalyzes the reversible transamination of branched-chain AMINO ACIDS to 2-oxoglutarate.Leucine: An essential branched-chain amino acid important for hemoglobin formation.

Overexpression of spermidine/spermine N1-acetyltransferase under the control of mouse metallothionein I promoter in transgenic mice: evidence for a striking post-transcriptional regulation of transgene expression by a polyamine analogue. (1/216)

We recently generated a transgenic mouse line overexpressing spermidine/spermine N1-acetyltransferase (SSAT) gene under its own promoter. The tissue polyamine pools of these animals were profoundly affected and the mice were hairless from early age. We have now generated another transgenic-mouse line overexpressing the SSAT gene under the control of a heavy-metal-inducible mouse metallothionein I (MT) promoter. Even in the absence of heavy metals, changes in the tissue polyamine pools indicated that a marked activation of polyamine catabolism had occurred in the transgenic animals. As with the SSAT transgenic mice generated previously, the mice of the new line (MT-SSAT) suffered permanent hair loss, but this occurred considerably later than in the previous SSAT transgenic animals. Liver was the most affected tissue in the MT-SSAT transgenic animals, revealed by putrescine overaccumulation, significant decrease in spermidine concentration and >90% reduction in the spermine pool. Even though hepatic SSAT mRNA accumulated to massive levels in non-induced transgenic animals, SSAT activity was only moderately elevated. Administration of ZnSO4 further elevated the level of hepatic SSAT message and induced enzyme activity, but not more than 2- to 3-fold. Treatment of the transgenic animals with the polyamine analogue N1,N11-diethylnorspermine (DENSPM) resulted in an immense induction, more than 40000-fold, of enzyme activity in the liver of transgenic animals, and minor changes in the SSAT mRNA level. Liver spermidine and spermine pools were virtually depleted within 1-2 days in response to the treatment with the analogue. The treatment also resulted in a marked mortality (up to 60%) among the transgenic animals which showed ultrastructural changes in the liver, most notably mitochondrial swelling, one of the earliest signs of cell injury. These results indicated that, even without its own promoter, SSAT is powerfully induced by the polyamine analogue through a mechanism that appears to involve a direct translational and/or heterogenous nuclear RNA processing control. It is likewise significant that overexpression of SSAT renders the animals extremely sensitive to polyamine analogues.  (+info)

Novel gating mechanism of polyamine block in the strong inward rectifier K channel Kir2.1. (2/216)

Inward rectifying K channels are essential for maintaining resting membrane potential and regulating excitability in many cell types. Previous studies have attributed the rectification properties of strong inward rectifiers such as Kir2.1 to voltage-dependent binding of intracellular polyamines or Mg to the pore (direct open channel block), thereby preventing outward passage of K ions. We have studied interactions between polyamines and the polyamine toxins philanthotoxin and argiotoxin on inward rectification in Kir2.1. We present evidence that high affinity polyamine block is not consistent with direct open channel block, but instead involves polyamines binding to another region of the channel (intrinsic gate) to form a blocking complex that occludes the pore. This interaction defines a novel mechanism of ion channel closure.  (+info)

Polyamine biosynthesis inhibitors alter protein-protein interactions involving estrogen receptor in MCF-7 breast cancer cells. (3/216)

We investigated the effects of polyamine biosynthesis inhibition on the estrogenic signaling pathway of MCF-7 breast cancer cells using a protein-protein interaction system. Estrogen receptor (ER) linked to glutathione-S-transferase (GST) was used to examine the effects of two polyamine biosynthesis inhibitors, difluoromethylornithine (DFMO) and CGP 48664. ER was specifically associated with a 45 kDa protein in control cells. In cells treated with estradiol, nine proteins were associated with ER. Cells treated with polyamine biosynthesis inhibitors in the absence of estradiol retained the binding of their ER with a 45 kDa protein and the ER also showed low-affinity interactions with a number of cellular proteins; however, these associations were decreased by the presence of estradiol and the inhibitors. When samples from the estradiol+DFMO treatment group were incubated with spermidine prior to GST-ER pull down assay, an increased association of several proteins with ER was detected. The intensity of the ER-associated 45 kDa protein increased by 10-fold in the presence of 1000 microM spermidine. These results indicate a specific role for spermidine in ER association of proteins. Western blot analysis of samples eluted from GST-ER showed the presence of chicken ovalbumin upstream promoter-transcription factor, an orphan nuclear receptor, and the endogenous full-length ER. These results show that multiple proteins associate with ER and that the binding of some of these proteins is highly sensitive to intracellular polyamine concentrations. Overall, our results indicate the importance of the polyamine pathway in the gene regulatory function of estradiol in breast cancer cells.  (+info)

Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock. (4/216)

Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylornithine, the DNA binding capacity of the transcription factor HSF induced by heat shock undergoes a severe and prompt deactivation. Replenishment of the spermidine pool before heat shock re-establishes the DNA binding activity of HSF and the inducibility of hsp 70 mRNA. Similar to HSF, but with a different time-course, the DNA binding of the transcription factor AP-1 activated by heat shock is also impaired in spermidine-depleted cells and reversed by exogenous spermidine. STAT3 provides an example of a transcription factor slightly activated by heat shock but insensitive to polyamine decrease.  (+info)

Inhibition of polyamine synthesis arrests trichomonad growth and induces destruction of hydrogenosomes. (5/216)

Trichomonad parasites such as Tritrichomonas foetus produce large amounts of putrescine (1,4-diaminobutane), which is transported out of the cell via an antiport mechanism which results in the uptake of a molecule of spermine. The importance of putrescine to the survival of the parasite and its role in the biology of T. foetus was investigated by use of the putrescine analogue 1, 4-diamino-2-butanone (DAB). Growth of T. foetus in vitro was significantly inhibited by 20 mM DAB, which was reversed by the addition of exogenous 40 mM putrescine. High-performance liquid chromatography analysis of 20 mM DAB-treated T. foetus revealed that putrescine, spermidine, and spermine levels were reduced by 89, 52, and 43%, respectively, compared to those in control cells. The DAB treatment induced several ultrastructural alterations, which were primarily observed in the redox organelles termed hydrogenosomes. These organelles were progressively degraded, giving rise to large vesicles that displayed material immunoreactive with an antibody to beta-succinyl-coenzyme A synthetase, a hydrogenosomal enzyme. A protective role for polyamines as stabilizing agents in the trichomonad hydrogenosomal membrane is proposed.  (+info)

Antizyme2 is a negative regulator of ornithine decarboxylase and polyamine transport. (6/216)

The antizyme family consists of closely homologous proteins believed to regulate cellular polyamine pools. Antizyme1, the first described, negatively regulates ornithine decarboxylase, the initial enzyme in the biosynthetic pathway for polyamines. Antizyme1 targets ornithine decarboxylase for degradation and inhibits polyamine transport into cells, thereby diminishing polyamine pools. A polyamine-stimulated ribosomal frameshift is required for decoding antizyme1 mRNA. Recently, additional novel conserved members of the antizyme family have been described. We report here the properties of one of these, antizyme2. Antizyme2, like antizyme1, binds to ornithine decarboxylase and inhibits polyamine transport. Using a baculovirus expression system in cultured Sf21 insect cells, both antizymes were found to accelerate ornithine decarboxylase degradation. Expression of either antizyme1 or 2 in Sf21 cells also diminished their uptake of the polyamine spermidine. Both forms of antizyme can therefore function as negative regulators of polyamine production and transport. However, in contrast to antizyme1, antizyme2 has negligible ability to stimulate degradation of ornithine decarboxylase in a rabbit reticulocyte lysate.  (+info)

The non-enzymatic hydrolysis of oligoribonucleotides VI. The role of biogenic polyamines. (7/216)

Single-stranded oligoribonucleotides containing UA and CA phosphodiester bonds can be hydrolyzed specifically under non-enzymatic conditions in the presence of spermidine, a biogenic amine found in a wide variety of organisms. In the present study, the rate of oligonucleotide and tRNA(i)(Met)hydrolysis was measured in the presence of spermidine and other biogenic amines. It was found that spermine [H(3)N(+)(CH(2))(3)(+)NH(2)(CH(2))(4)(+)NH(2)(CH(2))(3)(+)NH(3)] and putrescine [H(3)N(+)(CH(2))(4)(+)NH(3)] can replace spermidine [H(3)N(+)-(CH(2))(4)(+)NH(2)(CH(2))(3)(+)NH(3)] to induce the hydrolysis. For all three polyamines, a bell-shaped cleavage rate versus concentration relationship was observed. The maximum rate of hydrolysis was achieved at 0.1, 1.0 and 10 mM spermine, spermidine and putrescine, respectively. Moreover, we found that the hydrolysis requires at least two linked amino groups since two aminoalcohols, 2-aminoethanol and 3-aminopropanol, were not able to induce the cleavage of the phospho-diester bond. The optimal cleavage rate of the oligo-ribonucleotides was observed when amino groups were separated by tri- or tetramethylene linkers. The methylation of the amino groups reduced the ability of diamines to induce oligoribonucleotide hydrolysis. Non-enzymatic cleavage of tRNA(i)(Met)from Lupinus luteus and tRNA(i)(Met)from Escherichia coli demonstrate that both RNAs hydrolyze as expected from principles derived from oligoribonucleotide models.  (+info)

Polyamine regulation of plasma membrane phospholipid flip-flop during apoptosis. (8/216)

During apoptosis, phosphatidylserine (PS) is moved from the plasma membrane inner leaflet to the outer leaflet where it triggers recognition and phagocytosis of the apoptotic cell. Although the mechanisms of PS appearance during apoptosis are not well understood, it is thought that declining activity of the aminophospholipid translocase and calcium-mediated, nonspecific flip-flop of phospholipids play a role. As previous studies in the erythrocyte ghost have shown that polyamines can alter flip-flop of phospholipids, we asked whether alterations in cellular polyamines in intact cells undergoing apoptosis would affect PS appearance, either by altering aminophospholipid translocase activity or phospholipid flip-flop. Cells of the human leukemic cell line, HL-60, were incubated with or without the ornithine decarboxylase inhibitor, difluoromethylornithine (DFMO), and induced to undergo apoptosis by ultraviolet irradiation. Whereas DFMO treatment resulted in profound depletion of putrescine and spermidine (but not spermine), it had no effect on caspase activity, DNA fragmentation, or plasma membrane vesiculation, typical characteristics of apoptosis. Notably, DFMO treatment prior to ultraviolet irradiation did not alter the decline in PS inward movement by the aminophospholipid translocase as measured by the uptake of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled PS detected in the flow cytometer. Conversely, the appearance of endogenous PS in the plasma membrane outer leaflet detected with fluorescein isothiocyanate-labeled annexin V and enhanced phospholipid flip-flop detected by the uptake of 1-palmitoyl-1-[6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)aminocaproyl]-sn-glycero-3-phosphocholine (NBD-PC) seen during apoptosis were significantly inhibited by prior DFMO treatment. Importantly, replenishment of spermidine, by treatment with exogenous putrescine to bypass the metabolic blockade by DFMO, restored both enhanced phospholipid flip-flop and appearance of PS during apoptosis. Such restoration was seen even in the presence of cycloheximide but was not seen when polyamines were added externally just prior to assay. Taken together, these data show that intracellular polyamines can modulate PS appearance resulting from nonspecific flip-flop of phospholipids across the plasma membrane during apoptosis.  (+info)

*List of MeSH codes (D02)

... biogenic polyamines MeSH D02.092.211.415.261 --- cadaverine MeSH D02.092.211.415.701 --- putrescine MeSH D02.092.211.415. ... biogenic monoamines MeSH D02.092.211.215.311 --- catecholamines MeSH D02.092.211.215.311.342 --- dopamine MeSH D02.092.211.215. ...

*Ligation (molecular biology)

... concentration of DNA can be artificially increased by adding condensing agents such as cobalt hexamine and biogenic polyamines ... Raae AJ, Kleppe RK, Kleppe K (December 15, 1975). "Kinetics and effect of salts and polyamines on T4 polynucleotide ligase". ...

*Biogenic amine

N-Dimethyltryptamine Other biogenic monoamines Trimethylamine Trimethylamine N-oxide Examples of notable biogenic polyamines ... A biogenic amine is a biogenic substance with one or more amine groups. They are basic nitrogenous compounds formed mainly by ... However, food containing high amounts of biogenic amines may have toxicological effects. Biogenic amines are naturally present ... Biogenic amines can be found in all foods containing proteins or free amino acids and are found in a wide range of food ...

*Magnetite

Biomagnetism is usually related to the presence of biogenic crystals of magnetite, which occur widely in organisms. These ... "KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis". Nature Communications. 6: ... Hence, the presence of trace levels of biogenic magnetite in virtually all human tissues examined suggests that similar ... together these data suggest a previously unknown two-molecule sensing mechanism in which KCNJ15/Kir4.2 couples with polyamines ...

*Agmatine

Polyamine metabolism. Agmatine is a precursor for polyamine synthesis, competitive inhibitor of polyamine transport, inducer of ... Huisman H, Wynveen P, Nichkova M, Kellermann G (2010). "Novel ELISAs for screening of the biogenic amines GABA, glycine, beta- ... Satriano J (2004). "Arginine pathways and the inflammatory response: interregulation of nitric oxide and polyamines: review ... synthesis and polyamine metabolism and this provides bases for further research into potential applications. Agmatine was ...
Glycosylated polyamines comprising of mono- or oligo-saccharides that are glycosidically linked to an aliphatic polyamine, and pharmaceutically acceptable salts, prodrugs and derivatives thereof are provided. An exemplary glycosylated polyamine has the following structure: ##STR1##Glycosylated polyamines, their pharmaceutically acceptable salts, prodrugs and derivatives are useful, for example, as anticancer compounds for the treatment of a variety of cancers. Methods for synthesis of glycosylated polyamines are disclosed. In addition, metal complexes of glycosylated polyamines, the preparation of such metal complexes, analytical methods using the metal complexes are provided. Methods for detecting equatorial and axial conformation of a group other than hydrogen at the C2 position of a saccharide molecule are also provided.
Water-soluble polyamines which (a) have an average molecular weight of above 1,000, (b) contain at least one grouping of the formulain whichZ denotes a --COOH or --SO.sub.3 H group andR represents an alkylene radical, possessing 1 to 7 C atoms, which is optionally substituted and/or interrupted by a carbonamido group,Bonded to a basic nitrogen atom, and which (c) are obtainable either by reaction of water-soluble low molecular polyamines with compounds which introduce acid groups and with compounds which are polyfunctional towards amino groups, or by reaction of water-soluble higher-molecular polyamines, having an amine equivalent of 43 to 700, in which a hydrogen atom is bonded to at least every twentieth basic nitrogen atom, with compounds which introduce acid groups and optionally with compounds which are polyfunctional towards amino groups; a process for their manufacture and their use as agents for increasing the retention of fibres, fillers and pigments
Complete information for OAZ1 gene (Protein Coding), Ornithine Decarboxylase Antizyme 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Tiburcio AF, Altabella T, Bitrián M and Alcázar R. (2014) The Roles of Polyamines during the lifespan of plants:from development to stress. Planta, doi: 10.1007/s00425-014-2055-9. online first March 2014 read here Alcazar, R.; Tiburcio, A. F. (2014) Polyamines in stress protection - applications in agriculture: Plant adaptation to environmental change: significance of amino acids and their derivatives Pages: 129-140. CABI; Wallingford; UK. ISBN: 978-1-78064-273-4. read 2013 ...
Polyamines are small, ubiquitous organic polycations that have been implicated in a wide variety of physiological functions including protein translation, membrane stabilization and cell proliferation (reviewed in Tabor and Tabor, 1984). As one might expect, biosynthesis of polyamines is essential for viability of both prokaryotic and eukaryotic cells. The most common polyamines are putrescine, spermidine and spermine, which contain two, three or four charged amine groups, respectively. Eukaryotic organisms contain all three of these amines at abundant levels (high micromolar to millimolar). Much attention has focused on the roles of polyamines in various disease processes. For instance, polyamines have been implicated in autoimmune disorders such as systemic lupus erythematosus (Brooks, 1994). Polyamines also accumulate in cancer cells, and high levels of polyamines are found in the urine from cancer patients (reviewed in Russel and Duri, 1978; Chanda and Ganguly, 1988). Increased levels of ...
An increasing body of evidence indicates that cellular polyamines play a critical role in maintenance of the intestinal epithelial integrity, but few specific functions of polyamines at cellular and molecular levels are defined. We (18) have recently reported that polyamines are implicated in regulation of the intestinal epithelial barrier function and that depletion of cellular polyamines increases epithelial paracellular permeability at least partially by inhibiting expression of adherens junctions in undifferentiated parental IEC-6 cells. The present studies further confirm our previous observations by demonstrating that polyamines are crucial for expression of adherens junctions in differentiated IEC-Cdx2L1 cells. The most significant of the new findings reported in this study, however, is that polyamines are required for normal function of tight junctions and that polyamines regulate expression of various tight junction proteins through different mechanisms. Polyamines regulate occludin ...
Polyamine transport actions have already been described in diverse multicellular systems, but their bioenergetic systems and molecular identification stay unclear. methylglyoxal bis(guanylhydrazone), NMDG, S2 (Schneider range 2) cells, the initial such activity referred to within a model hereditary system, and weve characterized its kinetics, ionic requirements and pharmacological profile. This activity displays particular affinity for spermine and spermidine, however, not putrescine, would depend on H+, however, not Na+, and displays a pharmacological profile strikingly identical to that from the Slc22 (solute carrier 22) category of solute companies. These data will facilitate additional tests for the molecular id and characterization from the spermine/spermidine transporter in as well as perhaps in various other species aswell. MATERIALS AND Strategies Reagents [14C]Spermine tetrahydrochloride (113?Ci/mol) and [14C]spermdine trihydrochoride (112?Ci/mol) had been extracted from Amersham ...
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Looking for Hexamine? Find out information about Hexamine. hexamine, or urotropine, colorless crystals with a sweet taste that carbonize at 280° C and sublime in vacuum above 230° C. Hexamethylenetetramine is highly... Explanation of Hexamine
A stable isotope labelling assisted LC-MS method was developed for highly sensitive quantitation of polyamines in roots and leaves of rice plants under cadmium stress. Successful quantitation of polyamines in minute amounts of plant extracts allowed the detection of spatial and temporal changes in an organ/t
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phdthesis{8467fc6f-8c20-4634-9e13-8c10c0b1c8ef, abstract = {Our research group has previously shown that treatment with the polyamine biosynthesis inhibitors a-difluoromethylornithine (DFMO) or amidinoindan-1-one 2´-amidinohydrazone (CGP 48664) inhibited S phase progression before any other cell cycle phase was affected. This study was undertaken to further investigate the role of polyamines in the regulation of S phase progression and DNA synthesis. I have found that treatment with the polyamine analog <i>N</i><sup>1</sup>,<i>N</i><sup>11</sup>-diethylnorspermine (DENSPM) also caused a prolongation of the S phase. The common denominator for DFMO, CGP 48664, or DENSPM treatment is a depletion of the cellular spermidine pool. CGP 48664 and DENSPM in addition deplete the spermine pool. CGP 48664 or DENSPM treatment prolonged the S phase more than did DFMO treatment. Thus, mainly spermine but also spermidine may have a function in S phase ...
Polyamines are essential organic polycations which have been implicated in various cellular processes. Cellular polyamine concentrations are regulated mainly at the level of synthesis but also at the level of catabolism and export. Elevated cellular polyamine levels lead to the induction of antizyme translation by a highly conserved mechanism involving +1 ribosomal frameshifting. The antizyme targets ornithine decarboxylase (ODC), the rate limiting enzyme in polyamine biosynthesis for ubiquitin-independent degradation by the 26S proteasome. The N-terminal degron of ODC and the antizyme are essential for the effective degradation of ODC. Polyamines also regulate antizyme levels by blocking its ubiquitin-dependent protein degradation. In this study, the mechanisms of regulating polyamine levels by the antizyme are dissected ...
A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and their related monoacetyl derivatives in stomach tissue. The method is based on HPLC using octane sulfonate as an ion-pairing agent employed in acetate buffers at pH 4.5. The application is accompanied with fluorescence detection followed by post-column derivatization with o-phthaldialdehyde at room temperature (20±0.5°C). N1- and N8-acetylspermidines (ASPD) can be determined with this method in the same run without performing any special procedures or pre-purification in concentrations exceeding 8.5 pmoles. The variability in reproducibility of the day-today precision and duplicate determination, and simultaneous determination of standard mixture and biological samples were found < 2%. The mean (± s.e.mean) retention times (n=12) for putrescine (Put), N1-ASPD, N8-ASPD, spermidine (Spd) and spermine (Spm) are 8.97±0.025; 17.64±0.063; 18.99±0.133; 28.20±0.070 and 39.81±0
Polyamines are small cationic molecules that in adult brain are connected to neuronal signaling by regulating inward-rectifier K+-channels and different glutamate receptors. Antizyme inhibitors (AZINs) regulate the cellular uptake of polyamines and activate ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis. Elevated levels of ODC activity and polyamines are detected in various brain disorders including stroke and Alzheimers disease (AD).. We originally reported a novel brain- and testis-specific AZIN, called AZIN2, the distribution of which we have now studied in normal and diseased human brain by in situ hybridization and immunohistochemistry. We found the highest accumulation of AZIN2 in a pearl-on-the-string-like distribution along the axons in both the white and gray matter. AZIN2 was also detected in a vesicle-like distribution in the somas of selected cortical pyramidal neurons. Double-immunofluorescence staining revealed co-localization of AZIN2 and N-methyl ...
Viruses are suspected of significant roles in autoimmune diseases but the mechanisms are unclear. We get some insight by considering demands a virus places on host cells. Viruses require production of their own proteins, RNA and/or DNA, but also production of additional cellular machinery, such as ribosomes, to handle the increased demands. Since the nucleolus is a major site of RNA processing and ribonucleoprotein assembly, nucleoli are targeted by viruses, directly when viral RNA and proteins enter the nucleolus and indirectly when viruses induce increased expression of cellular polyamine genes. Polyamines are at high levels in nucleoli to assist in RNA folding. The size and activity of nucleoli increase directly with increases in polyamines. Nucleolar expansion due to abnormal increases in polyamines could disrupt nearby chromatin, such as the inactive X chromosome, leading to expression of previously sequestered DNA. Sudden expression of a large concentration of Alu elements from the disrupted
Increased polyamine biosynthesis activity and an active polyamine transport system are characteristics of many cancer cell lines and polyamine depletion has been shown to be a viable anticancer strategy. Polyamine levels can be depleted by difluoromethylornithine (DFMO), an inhibitor of the key polyamine biosynthesis enzyme ornithine decarboxylase (ODC). However, malignant cells frequently circumvent DFMO therapy by up-regulating polyamine import. Therefore, there is a need to develop compounds that inhibit polyamine transport. Collectively, DFMO and a polyamine transport inhibitor (PTI) provide the basis for a combination therapy leading to effective intracellular polyamine depletion. We have previously shown that the pattern of uptake of a series of polyamine analogues in a Drosophila model epithelium shares many characteristics with mammalian cells, indicating a high degree of similarity between the mammalian and Drosophila polyamine transport systems. In this report, we focused on the utility of the
TY - JOUR. T1 - Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells. AU - Bethell, Delia R.. AU - Pegg, Anthony E.. PY - 1981/9/16. Y1 - 1981/9/16. N2 - When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth.. AB - When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of ...
Polyamines are ubiquitous components of all eukaryotic cells and are found in brain tissue. A number of studies have shown that changes in the levels of polyamines occur following cerebral ischaemia. However, whether polyamines are neurotoxic or neuroprotective in ischaemia still remains unclear, although most of the evidence points to a toxic effect. The polyamine/NMDA antagonist N1-dansyl-spermine has been shown to be neuroprotective in a global ischaemia model. However, at the onset of the present study, the neuroprotective potential of this compound was not known in using focal ischaemia models, which are of more clinical relevance. Very recently, more novel polyamine analogues (BU31b, BU37b, BU33b, BU36b and BU43b) have been synthesized. These compounds, with the exception of BU37b, have shown some polyamine antagonist potential in vivo. Therefore these polyamine analogues are candidate neuroprotective agents. The pre-ischaemic effect of all these compounds mentioned above was investigated ...
Polyamines are nitrogenous, growth regulator like compounds, which are present in all living organisms. In plants, polyamines affect, for example, cell division, differentiation and stress tolerance. The project is coordinated by Metla and funded by the Academy of Finland. In laboratory experiments the roots of young seedlings of Scots pine were inoculated with the mycelium of S. variegatus and the development of the mycorrhizal interaction and changes in polyamine concentrations were monitored for five weeks. In the presence of the mycorrhizal fungus, the gene essential for the formation of polyamines was activated in wide areas of the developing root vascular tissue of the young Scots pine seedlings. The results show that the fungus induces the synthesis of plant polyamines and that polyamines play an important role in the formation of the symbiosis between S. variegatus and Scots pine seedlings. Mycorrhizal fungi are able to produce different amounts of polyamines. The results encourage ...
Polyamines in stress tolerance. Polyamines are small aliphatic compounds present in all living organisms. Polyamine biosynthesis is an evolutionary conserved pathway under an apparent purifying selection. Even though their essential role in stress tolerance is genetically demonstrated, molecular and signalling mechanisms underlying cell protection are relatively obscure. I am interested in gaining insight into the role of polyamines in stress tolerance and signalling (including developmental pathways) using advanced genetic approaches in plant science and exploring direct applications of polyamine content regulation (by means of natural variation) in crop breeding ...
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TY - JOUR. T1 - Growth arrest- and polyamine-dependent expression of spermidine/spermine N1-acetyltransferase in human tumor cells. AU - Ignatenko, Natalia. AU - Gerner, Eugene W.. PY - 1996/4. Y1 - 1996/4. N2 - Polyamines are essential for optimal cell growth. The regulation of polyamine biosynthetic, but not catabolic, enzymes has been studied in detail. Because intracellular polyamine contents depend on both synthesis and catabolism, we studied the regulation of spermidine/spermine N1- acetyltransferase (N1SSAT), the first enzyme in polyamine catabolism. Steady-state RNA levels of N1SSAT increased 3-5 fold as human colon tumor- derived HCT116 cells traversed the log phase and entered the plateau phase. Depletion of cellular polyamines, using α-difluoromethylornithine, caused a decrease in the steady-state levels of both the 1.3-kb N1SSAT transcript and its 3.5-kb precursor, without affecting the stability of either RNA. N1SSAT enzyme activity was low in cells with normal polyamine contents ...
Inhibition of polyamine biosynthesis and/or the perturbation of polyamine functionality have been exploited with success against parasitic diseases such as Trypanosoma infections. However, when the classical polyamine biosynthesis inhibitor, α-difluoromethylornithine, is used against the human malaria parasite, Plasmodium falciparum, it results in only a cytostatic growth arrest. Polyamine metabolism in this parasite has unique properties not shared by any other organism. These include the bifunctional arrangement of the catalytic decarboxylases and an apparent absence of the typical polyamine interconversion pathways implying different mechanisms for the regulation of polyamine homeostasis that includes the uptake of exogenous polyamines at least in vitro. These properties make polyamine metabolism an enticing drug target in P. falciparum provided that the physiological and functional consequences of polyamine metabolism perturbation are understood. This review highlights our current ...
Maintenance of intestinal mucosal epithelial integrity requires cellular polyamines that regulate expression of various genes involved in cell proliferation, growth arrest and apoptosis. Our previous studies have shown that polyamines are essential for expression of the c-myc gene and that polyamine-induced c-Myc plays a critical role in stimulation of normal IEC (intestinal epithelial cell) proliferation, but the exact downstream targets of induced c-Myc are still unclear. The p21Cip1 protein is a major player in cell cycle control, which is primarily regulated at the transcriptional level. The current study was designed to determine whether induced c-Myc stimulates normal IEC proliferation by repressing p21Cip1 transcription following up-regulation of polyamines. Overexpression of the ODC (ornithine decarboxylase) gene increased levels of cellular polyamines, induced c-Myc expression and inhibited p21Cip1 transcription, as indicated by repression of p21Cip1 promoter activity and a decrease in ...
ODC1 [ENSP00000234111]. Ornithine decarboxylase 1; Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis; Belongs to the Orn/Lys/Arg decarboxylase class-II family.. Synonyms: ODC1, ODC1p, hODC1, C9JG30, P11926 .... Linkouts: STRING Pharos UniProt OMIM ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Previous studies have shown that amine groups are ototoxic. The interaction between different polyamines and phospholipid vesicles was studied using vesicle aggregation and fluorescence techniques (DPH and ANS as the fluorescence probes). The results showed that the interaction between polyamines (spermine, spermidine and 1,3-diaminopropane) and acidic phospholipids (PS, PE, PI and PIP2) is an ionic one. The polyamine with the highest positive charges and the phospholipid with the highest content of negative groups showed the strongest ionic interaction. There was no indication of any hydrophobic interaction within the phospholipid bilayer. The strong interaction between amine groups and PIP2 support the proposal that the latter is crucially involved in aminoglycoside toxicity in the inner ear and kidney.
F14512, a Potent Antitumor Agent Targeting Topoisomerase II Vectored into Cancer Cells via the Polyamine Transport System Jean-Marc Barret, Anna Kruczynski,
Two genes,LAT1andOCT1, are likely to be involved in polyamine transport in Arabidopsis. Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine. Arabidopsis sp
Dont worry about hexamine cobalt chloride (Im assuming that you are doing some sort of 5-RACE). I did the type of ligation you are talking about recently using only the 10xbuffer provided with the buffer (50 mM Tris-HCl (pH 7.8), 10 mM MgCl2, 10 mM DTT and 1 mM ATP). I precipitated the oligo and ssDNA together and performed the ligation reaction in a minimal volume over night at 37C using 40 units of RNA ligase. It worked fine. But in other application I dont know whether hexamine cobalt chloride makes any difference... Marieluise Kirchner wrote: , used anyone the T4 RNA ligase for ssDNA / oligonucleotide-ligation? in , the buffer of my protocoll hexamine cobalt chloride is used. Why? Is the , effect really that big? Has hexamine cobalt chloride any bad effects in , further reactions like PCR or ligation into a vector? , The question is - should i use this hexamine cobalt chloride or better , not? , , thanks and a nice evening , , marie , , --- ...
Effect of nor-NOHA on arginase activity and amino acid levels. (A) Significant arginase inhibition was observed in cell lysates of CL-19 cultures treated with n
Author: Puleston, Daniel J. et al.; Genre: Journal Article; Published in Print: 2019; Title: Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation
SAT1 - SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 available for purchase from OriGene - Your Gene Company.
The objective of this project was to synthesize a protected spermidine derivative, which is a polyamine derivative. The spermidine that was to be synthesised is a N8 protected spermidine derivative.
Increased arginase activity contributes to airway nitric oxide (NO) deficiency in cystic fibrosis (CF). Whether down-stream products of arginase activity contribute to CF lung disease is currently unknown. The objective of this study was to test whether L-ornithine derived polyamines are present in CF airways and contribute to airway pathophysiology. Polyamine concentrations were measured in sputum of patients with CF and in healthy controls, using liquid chromatography-tandem mass spectrometry. The effect of spermine on airway smooth muscle mechanical properties was assessed in bronchial segments of murine airways, using a wire myograph. Sputum polyamine concentrations in stable CF patients were similar to healthy controls for putrescine and spermidine but significantly higher for spermine. Pulmonary exacerbations were associated with an increase in sputum and spermine levels. Treatment for pulmonary exacerbations resulted in decreases in arginase activity, L-ornithine and spermine concentrations in
The current study shows that topically applied dl-α- tocopherol reduced polyamine content in the skin of patients with large numbers of established AKs on the forearms. Elevated polyamine levels are characteristically seen in many types of neoplastic cells and tissues. OBrien and colleagues (29) and Peralta Soler and colleagues (30) have shown that in transgenic mice, overexpression of ornithine decarboxylase in skin leads to changes in tissue polyamine levels, particularly putrescine, and can modulate the development and maintenance of the neoplastic phenotype. Joshi (31) further hypothesized that polyamine synthesis is expected to promote endothelial cell proliferation and therefore angiogenesis. This reinforces the known role of polyamine biosynthesis in mediating skin tumor initiation and promotion (29-33).. The main findings of the present study can be summarized by the following statements. Daily topical application resulted in substantial uptake of free dl-α-tocopherol into the forearm ...
Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
The work in this thesis has covered three main topics; i) cytotoxic polyamine-conjugates ii) radiation protection polyamine-conjugates iii) polyamine conjugates which probe cellular uptake and DNA binding. The synthesis of these conjugates employed selective protection/deprotection steps, taking advantage of the BOC protecting groups regioselectivity of primary over secondary amines when reacting with naturally occurring polyamines.;After promising in vitro and in vivo results of the original spermidine-chlorambucil synthesised by Wheelhouse (1990), attempts were made to improve this compound. The terminal amines were ethylated. The new diethyl derivative showed greater inhibition of [C14] labelled spermidine entry into cultured cells, and improved cytotoxicity. Also a new novel cis-platin polyamine conjugate was synthesised and tested for cellular inhibition of radiolabelled spermidine and cytotoxicity. It failed to recognise the polyamine uptake receptor and gave poor [C14] spermidine ...
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HeLa cells were synchronized for S-phase DNA synthesis by the double thymidine-block procedure. A comparison was made of the polyamine content and S-phase DNA synthesis in cells from control cultures and cultures to which an inhibitor of polyamine biosynthesis, alpha-difluoromethylornithine, was added to the synchronization medium. Control cells showed a peak of synchronous DNA synthesis at 3 h and a maximum concentration of polyamines at 6-9 h after release of the second thymidine block. Cells from cultures containing the inhibitor were severely inhibited in the synthesis of DNA and contained no putrescine and only traces of spermidine while the spermine content was lowered by as much as 80%. Supplementation of cultures containing alpha-difluoromethylornithine with a polyamine, at the time of release of the second thymidine block, replenished the intracellular pool of the administered polyamine and partially restored S-phase DNA synthesis, with a lag of 3-6 h. Almost complete restoration of DNA ...
No expression and distribution patterns of polyamines (PAs), spermine, spermidine, and their precursor putrescine in mammalian hair follicle are available, although polyamines are known to correlate well with hair growth and epidermal tumor genesis. Immunohistochemistry (IHC) using our original two monoclonal antibodies (mAbs) ASPM-29 specific for spermine or spermidine, and APUT-32 specific for putrescine allowed us to detect immunoreactivity for polyamines in hair follicles from normal adult rats. A wide range of immunoreactivity for the total spermine and spermidine was observed in the compartments of hair follicle: The highest degree of immunoreactivity for polyamines was observed in the matrix, in the Huxleys layer, in the deeper Henles layer, and in the cuticle of the inner root sheath/the hair cuticle, while moderate immunoreactivity existed in the lower-to-mid cortex and the companion layer, followed by lower immunoreactivity in the outer root sheath, including the bulge region and in ...
Mammals vary in developmental maturity at birth; some are born altricial requiring significant parental investment, whereas others are more developmentally mature, or precocial. One thing they all have in common, however, is the consumption of milk as a first food. During the neonatal period, the mammalian gastrointestinal tract undergoes rapid maturation in preparation for the introduction of non-milk foods. Polyamine ingestion from milk is believed to have an essential role in this accelerated development of the small and large intestines.. To understand and identify specific functions of polyamines in mammalian infants, scientists performed experiments on non-human animals that included study groups that did not receive any polyamines-what better way to figure out what something does than to observe what happens when you take it away.. The earliest studies (during the 1990s) focused on rat models and found that rat pups receiving formula supplemented with polyamines (specifically spermine and ...
Polyamine analogues have been shown to have antitumor activity as single agents in multiple experimental model systems (7, 8, 9, 10, 11, 12, 13, 14) . Their ability to modulate response to chemotherapeutic agents is worthy of study. This study addressed the activity of two polyamine analogues, CPENSpm and CHENSpm, in combination with multiple chemotherapeutic agents in breast cancer cell lines. The chemotherapeutic agents used were selected because they: (a) have antitumor activity in breast cancer; (b) are currently in use in the treatment of breast cancer; and (c) represent a broad spectrum of mechanisms of action. They include alkylating agents (4HC), topoisomerase II inhibitors (doxorubicin), antimetabolites (5-FU and FdURd), antimitotic agents (vinorelbine, paclitaxel, and docetaxel), and the DNA-reactive agent, c-DDP, which causes both intra- and interstrand DNA adducts.. Synergistic combinations were identified using one or both of the polyamine analogues in all of the cell lines ...
In this study, we merged the rapidly increasing interest in epigenetic abnormalities in cancer and the possibilities for reversing these changes as a cancer therapy target, with the latest discoveries of enzymes that reverse key histone lysine modifications. The discovery of the amine oxidase homologue LSD1 as the first of these enzymes created an exciting new epigenetic target for which there has been an increasing effort to identify and design effective inhibitors (9-12). Our initial strategy for identifying effective inhibitors of LSD1 (3, 10, 14, 15, 28) was based on the structural similarity between LSD1 and the FAD-dependent SMO. This strategy proved to be effective and resulted in the successful identification of biguanide and bisguanidine polyamine analogues as LSD1 inhibitors (10). In the present study, we explored the oligoamines, another class of polyamine analogues that inhibit polyamine oxidases and that have high affinity for DNA based on the increased number of positively charged ...
1 mCi quantities of [Terminal Methylenes-3H(N)]-Spermidine Trihydrochloride are available for your research. Application of [3H]Spermidine can be found in: possible novel mechanism underlying temperature-dependent uptake in nuclear fractions in (murine) brain research, endogenous indoles as novel polyamine site ligands at the NMDA receptor complex in brain research, lysine-spermine conjugate inhibiting polyamine transport and cell growth when given with DFMO in experimental cell research, etc.. ...
Chrysarobin definition, a mixture of compounds obtained from Goa powder, used in the treatment of psoriasis and other skin conditions. See more.
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The enzyme is most active with L-methionine. It participates in the L-methionine salvage pathway from S-methyl-5-thioadenosine, a by-product of polyamine biosynthesis. T
J:147318 Lopez-Garcia C, Lopez-Contreras AJ, Cremades A, Castells MT, Marin F, Schreiber F, Penafiel R, Molecular and morphological changes in placenta and embryo development associated with the inhibition of polyamine synthesis during midpregnancy in mice. Endocrinology. 2008 Oct;149(10):5012-23 ...
The second one is the di-di process. You must start out with equal parts of DC and DF. That mixture is made before hand and stored as a precursor. The 50% DF is not part of the binary process, but is a separate step to produce the precursor. One assumes the HCl would be distilled out of that DC/DF mixture prior to storage and is not a factor at the time youre ready for the final Sarin-producing step. Then (on the diagram) it looks pretty simple - a mole of DC, a mole of DF and two moles of alcohol yield two moles of Sarin and two moles of hydrogen chloride ...
ATOC agreed a five year, £13 million deal with Fujitsu to refresh, enhance and streamline the hardware and applications technology used by RJIS, so that it could be supported until at least 2014. It also had to have the capacity to handle an increase in workload, largely driven by a massive growth in online sales (25% year-on-year) and greater use of Ticket-on-Departure vending-style machines used to collect ticket.
Az lommunka komplex feladat, pp gy sz ks g van az intellektusra, mint az rzelmekre. Az lmok meg rt se lehets ges, ha a jobb s a bal agyf lteke (az rz sek s az okos gondolkod s) egy ttm k dnek.
TY - JOUR. T1 - The mechanism of the inhibitory effect of polyamines on the induction of nitric oxide synthase role of aldehyde metabolites. AU - Szabo, Csaba. AU - Southan, G. J.. AU - Thiemermann, C.. AU - Vane, J. R.. PY - 1994. Y1 - 1994. N2 - 1. We have recently found that in the presence, but not in the absence, of foetal calf serum, spermine inhibits the production of nitric oxide (NO) in cultured J774.2 macrophages stimulated with bacterial endotoxin (lipopolysaccharide, LPS) or with γ-interferon (IFN), showing that polyamines may act as suppressants of NO-mediated immune functions. Here, we have studied the mechanisms and the specificity of this inhibitory action. 2. Other polyamines, as well as spermine, inhibit the formation of NO in cultured J774.2 macrophages, with the order of potency being spermine , spermidine ,, putrescine = cadaverine. This inhibition of NO formation is not due to any cytotoxic effect of these agents for they neither reduced mitochondrial respiration nor ...
Polyamines (spermidine and spermine) are required by living cells, but their functions are poorly understood. Mutants of Neurospora crassa with enhanced or diminished sensitivity to interference with polyamine synthesis, originally selected to study the regulation of the pathway, were found to have unexpected defects. A group of four non-allelic mutations, causing no interference with polyamine synthesis, each imparted spermidine auxotrophy to a genotype already partially impaired in spermidine synthesis. Strains carrying only the new mutations displayed unconditional delay or weakness at the onset of growth, but grew well thereafter and had a normal or overly active polyamine pathway. These mutants may have defects in vital macromolecular activities that are especially dependent upon the polyamines-activities that have not been identified with certainty in studies to date. Another group of mutants, selected as resistant to the polyamine inhibitor difluoromethylornithine (DFMO), had normal ...
TY - JOUR. T1 - Polyamine oxidase5 regulates arabidopsis growth through thermospermine oxidase activity. AU - Kim, Dong Wook. AU - Watanabe, Kanako. AU - Murayama, Chihiro. AU - Izawa, Sho. AU - Niitsu, Masaru. AU - Michael, Anthony J.. AU - Berberich, Thomas. AU - Kusano, Tomonobu. PY - 2014/8. Y1 - 2014/8. N2 - The major plant polyamines (PAs) are the tetraamines spermine (Spm) and thermospermine (T-Spm), the triamine spermidine, and the diamine putrescine. PA homeostasis is governed by the balance between biosynthesis and catabolism; the latter is catalyzed by polyamine oxidase (PAO). Arabidopsis (Arabidopsis thaliana) has five PAO genes, AtPAO1 to AtPAO5, and all encoded proteins have been biochemically characterized. All AtPAO enzymes function in the back-conversion of tetraamine to triamine and/or triamine to diamine, albeit with different PA specificities. Here, we demonstrate that AtPAO5 loss-of-function mutants (pao5) contain 2-fold higher T-Spm levels and exhibit delayed transition ...
The effects of inhibitors of polyamine synthesis on the invasive capacity of rat ascites hepatoma (LC-AH) cells were examined by in vitro assay of penetration of the LC-AH cells through a monolayer of calf pulmonary arterial endothelial (CPAE) cells. Pretreatment of LC-AH cells with α-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase, before seeding them onto a CPAE cell monolayer and culturing them for 24 h in the absence of DFMO decreased the number of penetrating tumor cells time and dose dependently (about 35% of the maximal inhibition) without affecting their viability or proliferative activity. DFMO treatment caused a marked decrease in the intracellular level of putrescine but not of spermidine or spermine. The DFMO-induced decreases in invasive capacity and putrescine level were almost completely reversed by the addition of putrescine to the medium during pretreatment with DFMO or invasion assay but were not affected by exogenous spermidine or spermine. No change ...
Putrescine, spermine, and spermidine may not have the most appetizing names, but these amino-acid derived molecules (called polyamines) are ingredients of all mammal milks. The presence of polyamines in milk is not surprising-putrescine, spermine, and spermidine are manufactured by all mammalian body cells, including mammary tissue. But polyamines are not accidental milk ingredients, passed on simply because they are ubiquitous in mammalian cells. Research from human and non-human animal models demonstrates that optimal nutrient absorption, the composition of the intestinal microbiome, and even food allergy may all depend on a sufficient supply of polyamines during the neonatal period. Milk polyamines, although odd in name, are essential for the proper maturation of the gastrointestinal tract in humans and other mammals. Read More... ...
Polyamines and biogenic amines are chemicals derived from amino acids. Polyamines are involved in intracellular signals. Biogenic amines function in intercellular communication. Epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) targets the pathways of biogenic amines and polyamines. This is of therapeutic interest in treating cancer. Tea is, also, a source of the amino acid, theanine, […]. View Post ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Polyammoniumcations have been used as DNA targeting ligands for a variety of DNA active reagents, for example radioprotectors and anti-tumour antibiotics, which do not rely on sequence specific binding for their activity. It is proposed that these ligands have a high affinity for DNA but exhibit an external (or loose) electrostatic binding interaction which enables them to remain mobile along DNA without dissociation of the drug: DNA complex. Polyammoniumcations based on the polytetraalkylammonium and polyprotonated ammonium salts of the naturally occurring polyamines spermine and spermidine were prepared. The synthesis of drug-ligand conjugates requires either selective mono-protection or mono-derivatisation of polyamines. These syntheses are problematic because of the combination of primary and secondary amine centres encountered and difficulties in handling the polyamines. Two strategies for achieving selective derivatisation were explored: the total synthesis of protected polyamines and ...
Principal Investigator:IGARASHI Kazuei, Project Period (FY):1997 - 1998, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:Biological pharmacy
Tiny molecular scaffolding that joins molecules together could be the key to our battle against antibiotic resistance. Research published in Bioorganic & Medicinal Chemistry Letters shows that carbon nanodot scaffolding assembled with small molecules called polyamines can kill some dangerous drug-resistant bacteria, including Acinetobacter baumanii and Klebsiella pneumonia.
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Biogenic Ketones Review: Does Biogenic Ketones really work for weight loss? What are the Benefits of Biogenic Ketones Supplement? Get All Answers ...
DESCRIPTION (provided by applicant): During Pneumocystis pneumonia (PcP), the number of alveolar macrophages (AMs) falls, impairing the innate immune system. This defect is mainly due to increased rate of apoptosis caused by elevated levels of intracellular polyamines. Preliminary results indicate that the levels of ornithine decarboxylase, antizyme inhibitor, and polyamine oxidase that are involved in polyamine synthesis and catabolism are increased during the infection. Increased expression of antizyme inhibitor (AZI) leads to increased polyamine production and uptake, thus raising intracellular polyamine and reactive oxygen species (ROS) levels. ROS damage the mitochondria of AMs, resulting in apoptosis. The central hypothesis of this project is that preventing the increase in intracellular polyamine levels will improve the vitality of AMs, leading to a more effective organism clearance. The long-term goal of this project is to understand the mechanism of pathogenesis of PcP and to develop ...
TY - JOUR. T1 - Crystal structure of human ornithine decarboxylase at 2.1 Å resolution. T2 - Structural insights to antizyme binding. AU - Almrud, Jeffrey J.. AU - Oliveira, Marcos A.. AU - Kern, Andrew D.. AU - Grishin, Nick V.. AU - Phillips, Margaret A.. AU - Hackert, Marvin L.. PY - 2000/1/7. Y1 - 2000/1/7. N2 - The polyamines spermidine and spermine are ubiquitous and required for cell growth and differentiation in eukaryotes. Ornithine decarboxylase (ODC, EC 4.1.1.17) performs the first step in polyamine biosynthesis, the decarboxylation of omithine to putrescine. Elevated polyamine levels can lead to down-regulation of ODC activity by enhancing the translation of antizyme mRNA, resulting in subsequent binding of antizyme to ODC monomers which targets ODC for proteolysis by the 26S proteasome. The crystal structure of ornithine decarboxylase from human liver has been determined to 2.1 Å resolution by molecular replacement using truncated mouse ODC (Δ425-461) as the search model and ...
Plant polyamines are catabolized by two classes of amine oxidases, the copper amine oxidases (CuAOs) and the flavin adenine dinucleotide (FAD)-dependent polyamine oxidases (PAOs). These enzymes differ to each other in substrate specificity, catalytic mechanism and subcellular localization. CuAOs and PAOs contribute to several physiological processes both through the control of polyamine homeostasis and as sources of biologically-active reaction products. CuAOs and PAOs have been found at high level in the cell wall of several species belonging to Fabaceae and Poaceae families, respectively, especially in tissues fated to undertake extensive wall loosening/stiffening events and/or in cells undergoing programmed cell death (PCD). Apoplastic CuAOs and PAOs have been shown to play a key role as a source of H2O2 in light- or developmentally-regulated differentiation events, thus influencing cell wall architecture and maturation as well as PCD. Moreover, growing evidence suggests a key role of intracellular
The regulation of SA to the changes of polyamine metabolism under salt stress in leaves of Yali Pear were studied. In order to reduce environmental effects on results, leaf disk tests were first used to confirm whether the salicylic acid affected the metabolism of polyamines. The results show that in the leaf disk experiment, exogenous salicylic acid changes the endogenous polyamine levels and different SA concentrations have different effects. Under the NaCI treatment the ADC activity increased significantly, but the ODC activity did not have a significant increase and was lower than ADC activity. Polyamine contents increased under the NaCI treatment at the low concentrations and did not increase or decrease with the NaCI concentration increasing. SA increased ODC activity in the control and NaCI treatment, did not significantly affect ADC activity, but increased the free polyamine contents under NaCI treatment ...
This study addresses how dietary folate manipulation impacts prostate cancer progression in an initiated mouse model. Our previous findings linking polyamine production to increased demand on folate intake to sustain prostate cancer cell proliferation (4) and maintain the genome (11) provided a compelling rationale to expect that prostate cancer would be extremely sensitive to availability of folate. Indeed, we found that mild dietary folate deficiency strongly blocked disease progression in TRAMP despite continued expression of the SV40 large T antigen and androgen receptor. Lack of polyamine production did not explain the lack of tumor growth on the deficient diet, as polyamine pools were not reduced by the diet (Supplementary Fig. S6). Indeed, polyamine levels are strictly maintained intracellularly, as shown by the large body of publications that in the past 20 years reported that targeting of polyamine biosynthesis to arrest cancer growth is largely ineffective due to complex compensatory ...
Polyamine transport is elevated in many tumor types, suggesting that toxic polyamine-drug conjugates could be targeted to cancer cells via the polyamine transporter (PAT). We have previously reported the use of Chinese hamster ovary (CHO) cells and its PAT-deficient mutant cell line, CHO-MG, to screen anthracene-polyamine conjugates for their PAT-selective targeting ability. We report here a novel Drosophila-based model for screening anthracene-polyamine conjugates in a developing and intact epithelium (Drosophila imaginal discs), wherein cell-cell adhesion properties are maintained. Data from the Drosophila assay are consistent with previous results in CHO cells, indicating that the Drosophila epithelium has a PAT with vertebrate-like characteristics. This assay will be of use to medicinal chemists interested in screening drugs that use PAT for cellular entry, and it offers the possibility of genetic dissection of the polyamine transport process, including identification of a Drosophila PAT.
NAD + -aminoaldehyde dehydrogenase candidates for Effect: of News, by Piers Robinson 4-aminobutyrate (GABA) and motivated staff an organisation, β-alanine production during terminal oxidation of Myth of News, Policy and Intervention by Piers Robinson polyamines in bichard report, apple fruit. Effect: Myth Foreign By Piers! E-mail address: [email protected] Department of Plant Agriculture, University of muscle Guelph, Guelph, Ontario N1G 2W1, Canada Search for more papers by of News, Foreign Robinson this author 1 Present address: NutriAg Ltd., 62 Arrow Rd, North York, Ontario M9M 2L8, Canada. Corresponding author. Ratio Dicidendi! Fax: +1 519 767 0755. Effect: The Policy And Intervention By Piers Robinson! First published: 18 August 2015 Full publication history DOI: 10.1016/j.febslet.2015.08.005 View/save citation Cited by (CrossRef): 0 articles Check for updates. Ratio Dicidendi! The last step of The CNN Effect: The of News, Foreign by Piers polyamine catabolism involves the of ...
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The polyamines spermine, spermidine, putrescine, cadaverine, etc. have been implicated in a variety of cellular functions. However, details of their mode of interaction with other ubiquitous biomolecules is not known. We have solved a few structures of polyamine-amino acid complexes to understand the nature and mode of their interactions. Here we report the structure of a complex of putrescine with DL-glutamic acid. Comparison of the structure with the structure of putrescine-L-glutamic acid complex reveals the high degree of similarity in the mode of interaction in the two complexes. Despite the presence of a centre of symmetry in the present case, the arrangement of molecules is strikingly similar to the L-glutamic acid complex.. ...
ODC1 Full-Length MS Protein Standard (NP_002530), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.
2YAU: A Gold-Containing Drug Against Parasitic Polyamine Metabolism: The X-Ray Structure of Trypanothione Reductase from Leishmania Infantum in Complex with Auranofin Reveals a Dual Mechanism of Enzyme Inhibition.
Arginase, Arginine Uptake and their Role in Leishmania (L.) amazonensis Physiology. Lucile Floeter-Winter, Universidade de São Paulo, Brazil ...
The TWiM team discusses how measles vaccination protects against other infectious diseases, and links between bacterial biofilms and colon cancer.
Control over chemical and biochemical processes by agents sensitive either to internal or external stimuli has attracted much attention in recent years. In particular photosensitive polyamines have been recently used to photo-trigger the hybridization/melting of DNA as well as to modify its intrinsic morphology. Th
Fineamin forms a strong, protective polyamines film on all the internal surfaces of the plant, protecting the system from oxygen and carbon dioxide actionand keeping the metal safe from corrosion.

RCSB PDB - SPM Ligand Summary PageRCSB PDB - SPM Ligand Summary Page

A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth ... Spermine is a polyamine, a small organic cations that is absolutely required for eukaryotic cell growth. Spermine, is normally ... Spermine is a polyamine. It is an organic molecule that is involved in cellular metabolism. ...
more infohttps://www.rcsb.org/ligand/SPM

IJMS  | Free Full-Text | Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism | HTMLIJMS | Free Full-Text | Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism | HTML

... and polyamine. It is clear that CD spectroscopy is extremely sensitive and relatively inexpensive, as compared with other ... DNA interaction with antitumor polyamine analogues: A comparison with biogenic polyamines. Biomacromolecules 2008, 9, 2712-2718 ... Ouameur, A.A.; Bourassa, P.; Tajmir-Riahi, H.A. Probing tRNA interaction with biogenic polyamines. RNA 2010, 16, 1968-1979. [ ... Ouameur, A.A.; Tajmir-Riahi, H.A. Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine ...
more infohttp://www.mdpi.com/1422-0067/13/3/3394/htm

Technical Information | YMC CO., LTD.Technical Information | YMC CO., LTD.

Biogenic Polyamines without derivatization *Analytical. Related Product:Triart PFP Data No. F130703B_polyamines 2013/10/03 ...
more infohttp://www.ymc.co.jp/en/technical/

Products in Amino Acids, MP Biomedicals on Thomas ScientificProducts in Amino Acids, MP Biomedicals on Thomas Scientific

Putrescine is a biogenic polyamine and precursor of spermidine. It is formed via decarboxylation of ornithine or by ... Spermidine is biogenic polyamine formed from putrescine, a precursor of spermine. Spermidine has a role in cell growth ... Binds to the polyamine modulatory site of the NMDA receptor and potentiates NMDA-induced currents; precursor of spermidine. ...
more infohttp://www.thomassci.com/nav/cat1/aminoacids/manufacturer/mpbiomedicals/0

CyclooxygenaseCyclooxygenase

Polyamines and biogenic amines are chemicals derived from amino acids. Polyamines are involved in intracellular signals. ... Green Tea, Polyamines and Biogenic Amines.. Posted on January 14, 2014. by Dr. Gary Pack ... Epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) targets the pathways of biogenic amines and polyamines. ... Miscellaneous: Biogenic amines, Caffeine, Cyclooxygenase, Dopamine, l-DOPA, Lipoxygenase inhibitor, Methylxanthine, ...
more infohttps://www.rainbow.coop/nutritional-library/miscellaneous/cyclooxygenase/

Parkinsons disease Archives - Page 2 of 5 - Rainbow GroceryParkinson's disease Archives - Page 2 of 5 - Rainbow Grocery

Polyamines and biogenic amines are chemicals derived from amino acids. Polyamines are involved in intracellular signals. ... Green Tea, Polyamines and Biogenic Amines.. Posted on January 14, 2014. by Dr. Gary Pack ... Epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) targets the pathways of biogenic amines and polyamines. ... Miscellaneous: Biogenic amines, Caffeine, Cyclooxygenase, Dopamine, l-DOPA, Lipoxygenase inhibitor, Methylxanthine, ...
more infohttps://www.rainbow.coop/nutritional-library/condition/parkinsons-disease/page/2/

ODC1 | Cancer Genetics WebODC1 | Cancer Genetics Web

Biogenic polyamines spermine and spermidine participate in numerous cellular processes including transcription, RNA processing ... homogenate on polyamine biosynthesis and polyamine degradation as well as on resulting polyamine levels in HGC-27 human gastric ... METHODS: Dietary polyamine data were available for 188 of 267 patients completing the study. Total dietary polyamine content ... Polyamines are synthesised endogenously and obtained from dietary sources. Here we investigate dietary polyamine intake and ...
more infohttp://www.cancerindex.org/geneweb/ODC1.htm

Publications | Max Planck Institute for Biophysical ChemistryPublications | Max Planck Institute for Biophysical Chemistry

Ramirez, F. J.; Thomas, T. J.; Antony, T.; Ruiz-Chica, J.; Thomas, T.: Effects of aminooxy analogues of biogenic polyamines on ...
more infohttps://www.mpibpc.mpg.de/publications?year=2002

CiNii Articles - Hirose MinaCiNii Articles - Hirose Mina

Atmospheric CO_2 captured by biogenic polyamines is transferred as a possible substrate to Rubisco for the carboxylation ...
more infohttps://ci.nii.ac.jp/author?q=Hirose+Mina

Similar articles for PubMed (Select 6151349) - PubMed - NCBISimilar articles for PubMed (Select 6151349) - PubMed - NCBI

Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared ... Effect of polyamines on mitochondrial F-ATPase from Crithidia fasciculata and Trypanosoma cruzi. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?db=pubmed&cmd=link&linkname=pubmed_pubmed&uid=6151349

NMR of α‐synuclein-polyamine complexes elucidates the mechanism and kinetics of induced aggregation | The EMBO JournalNMR of α‐synuclein-polyamine complexes elucidates the mechanism and kinetics of induced aggregation | The EMBO Journal

Biogenic polyamines promote the aggregation of α‐synuclein and may constitute endogenous agents modulating the pathogenesis of ... A) No polyamine (0), and 100 μM putrescine (1), spermidine (2), spermine (3) and the synthetic polyamines 4‐4‐4 (4) and BE‐4‐4‐ ... A) The different polyamines are designated as in Figure 1. Solid lines, global fits (for each polyamine, assigning the same ... ii) a constant fraction, β, of monomer (free or aggregated) in a polyamine‐liganded form, dictated by polyamine type and ...
more infohttp://emboj.embopress.org/content/23/10/2039?ijkey=9f443b0d60b3282bff04b41701fc239e767fce48&keytype2=tf_ipsecsha

Sci. Pharm. | Free Full-Text | Simultaneous Determination of Different Polyamines and their Mono-Acetylated Derivatives in...Sci. Pharm. | Free Full-Text | Simultaneous Determination of Different Polyamines and their Mono-Acetylated Derivatives in...

Information on gastric tissue polyamines and their acetylated derivatives may be useful in understanding the mechanism of drugs ... A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and ... A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and ... Keywords: Endogenous polyamines; HPLC; Post-column derivatization; Rat; Gastric tissue Endogenous polyamines; HPLC; Post-column ...
more infohttp://www.mdpi.com/2218-0532/78/2/249

Biochemistry (Moscow) (v.77, #10) | www.chemweb.comBiochemistry (Moscow) (v.77, #10) | www.chemweb.com

Biogenic polyamines spermine and spermidine activate RNA polymerase and inhibit RNA helicase of hepatitis C virus by A. N. ... Influence of the biogenic polyamines spermine, spermidine, and putrescine as well as their derivatives on the replication ... These data suggest that biogenic polyamines may cause differently directed effects on the replication of the HCV genome in an ... Natural polyamines and their derivatives effectively inhibited the helicase reaction catalyzed by another enzyme of HCV ...
more infohttps://chemweb.com/articles/SV10541/0007700010

WO2013092506A1 - Hydroxy amino polymer and use thereof in polyurea/polyurethane tissue adhesives 
        - Google PatentsWO2013092506A1 - Hydroxy amino polymer and use thereof in polyurea/polyurethane tissue adhesives - Google Patents

... or other biogenic intermediates such as biogenic polyamines (spermine, Spermitin, putrescine or bioactive artificial polyamines ... New polyamines and their use in paints and coatings EP0810247A3 (en) 1996-05-29. 1997-12-10. Elf Atochem S.A.. Adhesive ...
more infohttps://patents.google.com/patent/WO2013092506A1/en

Ezine - spectroscopyNOW.comEzine - spectroscopyNOW.com

Biogenic polyamines that are toxic to humans can be measured in distilled drinks using a direct mass spectrometric method after ... Polyamines in distilled drinks: Host-guest complexation for mass spectrometry. Date: Nov 1, 2016 ...
more infohttp://www.spectroscopynow.com/ms/view/ezine.html?page=7

Marques, M. Paula M. | Estudo GeralMarques, M. Paula M. | Estudo Geral

Cytotoxic Activity of Metal Complexes of Biogenic Polyamines: Polynuclear Platinum(II) Chelates. Teixeira, Luísa J. ; Seabra, ...
more infohttps://estudogeral.sib.uc.pt/cris/rp/rp45263

Metabolomics/Metabolites/Amino Acids - Wikibooks, open books for an open worldMetabolomics/Metabolites/Amino Acids - Wikibooks, open books for an open world

Spermine- A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an ... Researcher concluded that PA2 regulates polyamine metabolism. Polyamine- are organic compounds having two or more primary amino ... A lack of PA2 led to decreased amounts of the polyamine. Examining polyamine synthesis showed that the different spermidine ... Polyamines such as spermine and spermidine are involved in DNA packaging. They are derived from methionine and ornithine by a ...
more infohttps://en.m.wikibooks.org/wiki/Metabolomics/Metabolites/Amino_Acids

Faculty Full ProfileFaculty Full Profile

Thermodynamics of the DNA binding of biogenic polyamines: Calorimetric and spectroscopic investigations. J. Chem. Thermodyn. ... Spectroscopic investigation on interaction of biogenic, Croton bonplandianum leaves extract mediated potential bactericidal ...
more infohttp://vidyasagar.ac.in/Faculty/Profile/Print?fac_u_id=Fac-CHEM-164

Frontiers | Role of Starter Cultures on the Safety of Fermented Meat Products | MicrobiologyFrontiers | Role of Starter Cultures on the Safety of Fermented Meat Products | Microbiology

Biogenic amines (BA) are potentially unsafe nitrogenous compounds that result from the decarboxylation of some amino acids. ... Biogenic amines (BA) are potentially unsafe nitrogenous compounds that result from the decarboxylation of some amino acids. ... On the other hand, Staphylococcus xylosus and Debaryomyces hansenii strains have been reported to degrade biogenic amines in ... particularly biogenic amines, nitrosamines, polycyclic aromatic hydrocarbons (PAH), and mycotoxins. ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00853/full

PublicationsPublications

Pallan, P. S.; Ganesh, K. N. DNA triple helix stabilization by bis guanidinyl analogs of biogenic polyamines. Biochem.Biophys. ... Nagamani, D.; Ganesh, K. N. Pyrrolidyl Polyamines: Branched, Chiral Polyamine Analogues That Stabilize DNA Duplexes and ... Prakash, T. P.; Barawkar, D. A.; Kumar, V. A.; Ganesh, K. N. Synthesis of site-specific oligonucleotide-polyamine conjugates. ... Stabilization of DNA structures through nucleobase modifications and bisguanidium polyamines. Indian J. Chem., Sect. B. 1997, ...
more infohttp://www.knganesh.in/Publications.htm

List of MeSH codes (D02) - WikipediaList of MeSH codes (D02) - Wikipedia

... biogenic polyamines MeSH D02.092.211.415.261 --- cadaverine MeSH D02.092.211.415.701 --- putrescine MeSH D02.092.211.415. ... biogenic monoamines MeSH D02.092.211.215.311 --- catecholamines MeSH D02.092.211.215.311.342 --- dopamine MeSH D02.092.211.215. ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D02)

Ligation (molecular biology) - WikipediaLigation (molecular biology) - Wikipedia

... concentration of DNA can be artificially increased by adding condensing agents such as cobalt hexamine and biogenic polyamines ... Raae AJ, Kleppe RK, Kleppe K (December 15, 1975). "Kinetics and effect of salts and polyamines on T4 polynucleotide ligase". ...
more infohttps://en.wikipedia.org/wiki/Ligation_(molecular_biology)

ICBMS - PublicationsICBMS - Publications

Diastereoselective Synthesis of a Dyn[3]arene with Distinct Binding Behaviors toward Linear Biogenic Polyamines. Donnier- ...
more infohttp://icbms.univ-lyon1.fr/csap/publications?iccaldate=2019-03-1
  • Greater polyamine charge (+2 → +5) correlated with increased affinity and enhancement of fibrillation, for which we propose a simple kinetic mechanism involving a dimeric nucleation center. (embopress.org)
  • Biogenic polyamines that are toxic to humans can be measured in distilled drinks using a direct mass spectrometric method after trapping them in inclusion complexes. (spectroscopynow.com)
  • conversely, the significant reductions in polyamine levels resulting from the topical DL-alpha-tocopherol application are consistent with reductions in tumorigenesis potential. (nih.gov)
  • We characterized the complexes of natural and synthetic polyamines with α‐synuclein by NMR and assigned the binding site to C‐terminal residues 109-140. (embopress.org)
  • Polyamines: Natural and engineered abiotic and biotic stress tolerance in plants. (ac.ir)
  • In our complexes, the Pt(II) or Pd(II) ion is not bound to ammonia as in cisplatin but it is bound to NH 2 and NH groups of linear polyamines. (stfc.ac.uk)