Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA.
A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.
A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.
A polyamine formed from putrescine. It is found in almost all tissues in association with nucleic acids. It is found as a cation at all pH values, and is thought to help stabilize some membranes and nucleic acid structures. It is a precursor of spermine.
A group of naturally occurring amines derived by enzymatic decarboxylation of the natural amino acids. Many have powerful physiological effects (e.g., histamine, serotonin, epinephrine, tyramine). Those derived from aromatic amino acids, and also their synthetic analogs (e.g., amphetamine), are of use in pharmacology.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Cell surface proteins that bind biogenic amines with high affinity and regulate intracellular signals which influence the behavior of cells. Biogenic amine is a chemically imprecise term which, by convention, includes the catecholamines epinephrine, norepinephrine, and dopamine, the indoleamine serotonin, the imidazolamine histamine, and compounds closely related to each of these.
A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.
An inhibitor of ORNITHINE DECARBOXYLASE, the rate limiting enzyme of the polyamine biosynthetic pathway.
An alpha-adrenergic sympathomimetic amine, biosynthesized from tyramine in the CNS and platelets and also in invertebrate nervous systems. It is used to treat hypotension and as a cardiotonic. The natural D(-) form is more potent than the L(+) form in producing cardiovascular adrenergic responses. It is also a neurotransmitter in some invertebrates.
A foul-smelling diamine formed by bacterial decarboxylation of lysine.
An enzyme that catalyzes the decarboxylation of S-adenosyl-L-methionine to yield 5'-deoxy-(5'-),3-aminopropyl-(1), methylsulfonium salt. It is one of the enzymes responsible for the synthesis of spermidine from putrescine. EC
Organic chemicals which have two amino groups in an aliphatic chain.
Biogenic amines having only one amine moiety. Included in this group are all natural monoamines formed by the enzymatic decarboxylation of natural amino acids.
An amino acid produced in the urea cycle by the splitting off of urea from arginine.
An indirect sympathomimetic. Tyramine does not directly activate adrenergic receptors, but it can serve as a substrate for adrenergic uptake systems and monoamine oxidase so it prolongs the actions of adrenergic transmitters. It also provokes transmitter release from adrenergic terminals. Tyramine may be a neurotransmitter in some invertebrate nervous systems.
Camellia sinensis L. (formerly Thea sinensis) is an evergreen Asiatic shrub of the THEACEAE family. The infusion of leaves of this plant is used as Oriental TEA which contains CAFFEINE; THEOPHYLLINE; and epigallocatechin gallate.
A plant genus in the family THEACEAE, order THEALES best known for CAMELLIA SINENSIS which is the source of Oriental TEA.
The infusion of leaves of CAMELLIA SINENSIS (formerly Thea sinensis) as a beverage, the familiar Asian tea, which contains CATECHIN (especially epigallocatechin gallate) and CAFFEINE.
A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed)
An antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.
A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.
A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Substances that reduce the growth or reproduction of BACTERIA.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
A peptide which is a homopolymer of lysine.
An alkaloid from SOLANACEAE, especially DATURA and SCOPOLIA. Scopolamine and its quaternary derivatives act as antimuscarinics like ATROPINE, but may have more central nervous system effects. Among the many uses are as an anesthetic premedication, in URINARY INCONTINENCE, in MOTION SICKNESS, as an antispasmodic, and as a mydriatic and cycloplegic.
A complication of kidney diseases characterized by cell death involving KIDNEY PAPILLA in the KIDNEY MEDULLA. Damages to this area may hinder the kidney to concentrate urine resulting in POLYURIA. Sloughed off necrotic tissue may block KIDNEY PELVIS or URETER. Necrosis of multiple renal papillae can lead to KIDNEY FAILURE.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
The family Sturnidae, in the order PASSERIFORMES. The starling family also includes mynahs and oxpeckers.
Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
Family of retrovirus-associated DNA sequences (myc) originally isolated from an avian myelocytomatosis virus. The proto-oncogene myc (c-myc) codes for a nuclear protein which is involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Truncation of the first exon, which appears to regulate c-myc expression, is crucial for tumorigenicity. The human c-myc gene is located at 8q24 on the long arm of chromosome 8.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent.
Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.
The 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholanic acid family of bile acids in man, usually conjugated with glycine or taurine. They act as detergents to solubilize fats for intestinal absorption, are reabsorbed by the small intestine, and are used as cholagogues and choleretics.
A bile acid, usually conjugated with either glycine or taurine. It acts as a detergent to solubilize fats for intestinal absorption and is reabsorbed by the small intestine. It is used as cholagogue, a choleretic laxative, and to prevent or dissolve gallstones.
A major primary bile acid produced in the liver and usually conjugated with glycine or taurine. It facilitates fat absorption and cholesterol excretion.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
An epimer of chenodeoxycholic acid. It is a mammalian bile acid found first in the bear and is apparently either a precursor or a product of chenodeoxycholate. Its administration changes the composition of bile and may dissolve gallstones. It is used as a cholagogue and choleretic.

Overexpression of spermidine/spermine N1-acetyltransferase under the control of mouse metallothionein I promoter in transgenic mice: evidence for a striking post-transcriptional regulation of transgene expression by a polyamine analogue. (1/216)

We recently generated a transgenic mouse line overexpressing spermidine/spermine N1-acetyltransferase (SSAT) gene under its own promoter. The tissue polyamine pools of these animals were profoundly affected and the mice were hairless from early age. We have now generated another transgenic-mouse line overexpressing the SSAT gene under the control of a heavy-metal-inducible mouse metallothionein I (MT) promoter. Even in the absence of heavy metals, changes in the tissue polyamine pools indicated that a marked activation of polyamine catabolism had occurred in the transgenic animals. As with the SSAT transgenic mice generated previously, the mice of the new line (MT-SSAT) suffered permanent hair loss, but this occurred considerably later than in the previous SSAT transgenic animals. Liver was the most affected tissue in the MT-SSAT transgenic animals, revealed by putrescine overaccumulation, significant decrease in spermidine concentration and >90% reduction in the spermine pool. Even though hepatic SSAT mRNA accumulated to massive levels in non-induced transgenic animals, SSAT activity was only moderately elevated. Administration of ZnSO4 further elevated the level of hepatic SSAT message and induced enzyme activity, but not more than 2- to 3-fold. Treatment of the transgenic animals with the polyamine analogue N1,N11-diethylnorspermine (DENSPM) resulted in an immense induction, more than 40000-fold, of enzyme activity in the liver of transgenic animals, and minor changes in the SSAT mRNA level. Liver spermidine and spermine pools were virtually depleted within 1-2 days in response to the treatment with the analogue. The treatment also resulted in a marked mortality (up to 60%) among the transgenic animals which showed ultrastructural changes in the liver, most notably mitochondrial swelling, one of the earliest signs of cell injury. These results indicated that, even without its own promoter, SSAT is powerfully induced by the polyamine analogue through a mechanism that appears to involve a direct translational and/or heterogenous nuclear RNA processing control. It is likewise significant that overexpression of SSAT renders the animals extremely sensitive to polyamine analogues.  (+info)

Novel gating mechanism of polyamine block in the strong inward rectifier K channel Kir2.1. (2/216)

Inward rectifying K channels are essential for maintaining resting membrane potential and regulating excitability in many cell types. Previous studies have attributed the rectification properties of strong inward rectifiers such as Kir2.1 to voltage-dependent binding of intracellular polyamines or Mg to the pore (direct open channel block), thereby preventing outward passage of K ions. We have studied interactions between polyamines and the polyamine toxins philanthotoxin and argiotoxin on inward rectification in Kir2.1. We present evidence that high affinity polyamine block is not consistent with direct open channel block, but instead involves polyamines binding to another region of the channel (intrinsic gate) to form a blocking complex that occludes the pore. This interaction defines a novel mechanism of ion channel closure.  (+info)

Polyamine biosynthesis inhibitors alter protein-protein interactions involving estrogen receptor in MCF-7 breast cancer cells. (3/216)

We investigated the effects of polyamine biosynthesis inhibition on the estrogenic signaling pathway of MCF-7 breast cancer cells using a protein-protein interaction system. Estrogen receptor (ER) linked to glutathione-S-transferase (GST) was used to examine the effects of two polyamine biosynthesis inhibitors, difluoromethylornithine (DFMO) and CGP 48664. ER was specifically associated with a 45 kDa protein in control cells. In cells treated with estradiol, nine proteins were associated with ER. Cells treated with polyamine biosynthesis inhibitors in the absence of estradiol retained the binding of their ER with a 45 kDa protein and the ER also showed low-affinity interactions with a number of cellular proteins; however, these associations were decreased by the presence of estradiol and the inhibitors. When samples from the estradiol+DFMO treatment group were incubated with spermidine prior to GST-ER pull down assay, an increased association of several proteins with ER was detected. The intensity of the ER-associated 45 kDa protein increased by 10-fold in the presence of 1000 microM spermidine. These results indicate a specific role for spermidine in ER association of proteins. Western blot analysis of samples eluted from GST-ER showed the presence of chicken ovalbumin upstream promoter-transcription factor, an orphan nuclear receptor, and the endogenous full-length ER. These results show that multiple proteins associate with ER and that the binding of some of these proteins is highly sensitive to intracellular polyamine concentrations. Overall, our results indicate the importance of the polyamine pathway in the gene regulatory function of estradiol in breast cancer cells.  (+info)

Influence of polyamines on DNA binding of heat shock and activator protein 1 transcription factors induced by heat shock. (4/216)

Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylornithine, the DNA binding capacity of the transcription factor HSF induced by heat shock undergoes a severe and prompt deactivation. Replenishment of the spermidine pool before heat shock re-establishes the DNA binding activity of HSF and the inducibility of hsp 70 mRNA. Similar to HSF, but with a different time-course, the DNA binding of the transcription factor AP-1 activated by heat shock is also impaired in spermidine-depleted cells and reversed by exogenous spermidine. STAT3 provides an example of a transcription factor slightly activated by heat shock but insensitive to polyamine decrease.  (+info)

Inhibition of polyamine synthesis arrests trichomonad growth and induces destruction of hydrogenosomes. (5/216)

Trichomonad parasites such as Tritrichomonas foetus produce large amounts of putrescine (1,4-diaminobutane), which is transported out of the cell via an antiport mechanism which results in the uptake of a molecule of spermine. The importance of putrescine to the survival of the parasite and its role in the biology of T. foetus was investigated by use of the putrescine analogue 1, 4-diamino-2-butanone (DAB). Growth of T. foetus in vitro was significantly inhibited by 20 mM DAB, which was reversed by the addition of exogenous 40 mM putrescine. High-performance liquid chromatography analysis of 20 mM DAB-treated T. foetus revealed that putrescine, spermidine, and spermine levels were reduced by 89, 52, and 43%, respectively, compared to those in control cells. The DAB treatment induced several ultrastructural alterations, which were primarily observed in the redox organelles termed hydrogenosomes. These organelles were progressively degraded, giving rise to large vesicles that displayed material immunoreactive with an antibody to beta-succinyl-coenzyme A synthetase, a hydrogenosomal enzyme. A protective role for polyamines as stabilizing agents in the trichomonad hydrogenosomal membrane is proposed.  (+info)

Antizyme2 is a negative regulator of ornithine decarboxylase and polyamine transport. (6/216)

The antizyme family consists of closely homologous proteins believed to regulate cellular polyamine pools. Antizyme1, the first described, negatively regulates ornithine decarboxylase, the initial enzyme in the biosynthetic pathway for polyamines. Antizyme1 targets ornithine decarboxylase for degradation and inhibits polyamine transport into cells, thereby diminishing polyamine pools. A polyamine-stimulated ribosomal frameshift is required for decoding antizyme1 mRNA. Recently, additional novel conserved members of the antizyme family have been described. We report here the properties of one of these, antizyme2. Antizyme2, like antizyme1, binds to ornithine decarboxylase and inhibits polyamine transport. Using a baculovirus expression system in cultured Sf21 insect cells, both antizymes were found to accelerate ornithine decarboxylase degradation. Expression of either antizyme1 or 2 in Sf21 cells also diminished their uptake of the polyamine spermidine. Both forms of antizyme can therefore function as negative regulators of polyamine production and transport. However, in contrast to antizyme1, antizyme2 has negligible ability to stimulate degradation of ornithine decarboxylase in a rabbit reticulocyte lysate.  (+info)

The non-enzymatic hydrolysis of oligoribonucleotides VI. The role of biogenic polyamines. (7/216)

Single-stranded oligoribonucleotides containing UA and CA phosphodiester bonds can be hydrolyzed specifically under non-enzymatic conditions in the presence of spermidine, a biogenic amine found in a wide variety of organisms. In the present study, the rate of oligonucleotide and tRNA(i)(Met)hydrolysis was measured in the presence of spermidine and other biogenic amines. It was found that spermine [H(3)N(+)(CH(2))(3)(+)NH(2)(CH(2))(4)(+)NH(2)(CH(2))(3)(+)NH(3)] and putrescine [H(3)N(+)(CH(2))(4)(+)NH(3)] can replace spermidine [H(3)N(+)-(CH(2))(4)(+)NH(2)(CH(2))(3)(+)NH(3)] to induce the hydrolysis. For all three polyamines, a bell-shaped cleavage rate versus concentration relationship was observed. The maximum rate of hydrolysis was achieved at 0.1, 1.0 and 10 mM spermine, spermidine and putrescine, respectively. Moreover, we found that the hydrolysis requires at least two linked amino groups since two aminoalcohols, 2-aminoethanol and 3-aminopropanol, were not able to induce the cleavage of the phospho-diester bond. The optimal cleavage rate of the oligo-ribonucleotides was observed when amino groups were separated by tri- or tetramethylene linkers. The methylation of the amino groups reduced the ability of diamines to induce oligoribonucleotide hydrolysis. Non-enzymatic cleavage of tRNA(i)(Met)from Lupinus luteus and tRNA(i)(Met)from Escherichia coli demonstrate that both RNAs hydrolyze as expected from principles derived from oligoribonucleotide models.  (+info)

Polyamine regulation of plasma membrane phospholipid flip-flop during apoptosis. (8/216)

During apoptosis, phosphatidylserine (PS) is moved from the plasma membrane inner leaflet to the outer leaflet where it triggers recognition and phagocytosis of the apoptotic cell. Although the mechanisms of PS appearance during apoptosis are not well understood, it is thought that declining activity of the aminophospholipid translocase and calcium-mediated, nonspecific flip-flop of phospholipids play a role. As previous studies in the erythrocyte ghost have shown that polyamines can alter flip-flop of phospholipids, we asked whether alterations in cellular polyamines in intact cells undergoing apoptosis would affect PS appearance, either by altering aminophospholipid translocase activity or phospholipid flip-flop. Cells of the human leukemic cell line, HL-60, were incubated with or without the ornithine decarboxylase inhibitor, difluoromethylornithine (DFMO), and induced to undergo apoptosis by ultraviolet irradiation. Whereas DFMO treatment resulted in profound depletion of putrescine and spermidine (but not spermine), it had no effect on caspase activity, DNA fragmentation, or plasma membrane vesiculation, typical characteristics of apoptosis. Notably, DFMO treatment prior to ultraviolet irradiation did not alter the decline in PS inward movement by the aminophospholipid translocase as measured by the uptake of 6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminocaproyl] (NBD)-labeled PS detected in the flow cytometer. Conversely, the appearance of endogenous PS in the plasma membrane outer leaflet detected with fluorescein isothiocyanate-labeled annexin V and enhanced phospholipid flip-flop detected by the uptake of 1-palmitoyl-1-[6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)aminocaproyl]-sn-glycero-3-phosphocholine (NBD-PC) seen during apoptosis were significantly inhibited by prior DFMO treatment. Importantly, replenishment of spermidine, by treatment with exogenous putrescine to bypass the metabolic blockade by DFMO, restored both enhanced phospholipid flip-flop and appearance of PS during apoptosis. Such restoration was seen even in the presence of cycloheximide but was not seen when polyamines were added externally just prior to assay. Taken together, these data show that intracellular polyamines can modulate PS appearance resulting from nonspecific flip-flop of phospholipids across the plasma membrane during apoptosis.  (+info)

Glycosylated polyamines comprising of mono- or oligo-saccharides that are glycosidically linked to an aliphatic polyamine, and pharmaceutically acceptable salts, prodrugs and derivatives thereof are provided. An exemplary glycosylated polyamine has the following structure: ##STR1##Glycosylated polyamines, their pharmaceutically acceptable salts, prodrugs and derivatives are useful, for example, as anticancer compounds for the treatment of a variety of cancers. Methods for synthesis of glycosylated polyamines are disclosed. In addition, metal complexes of glycosylated polyamines, the preparation of such metal complexes, analytical methods using the metal complexes are provided. Methods for detecting equatorial and axial conformation of a group other than hydrogen at the C2 position of a saccharide molecule are also provided.
Water-soluble polyamines which (a) have an average molecular weight of above 1,000, (b) contain at least one grouping of the formulain whichZ denotes a --COOH or --SO.sub.3 H group andR represents an alkylene radical, possessing 1 to 7 C atoms, which is optionally substituted and/or interrupted by a carbonamido group,Bonded to a basic nitrogen atom, and which (c) are obtainable either by reaction of water-soluble low molecular polyamines with compounds which introduce acid groups and with compounds which are polyfunctional towards amino groups, or by reaction of water-soluble higher-molecular polyamines, having an amine equivalent of 43 to 700, in which a hydrogen atom is bonded to at least every twentieth basic nitrogen atom, with compounds which introduce acid groups and optionally with compounds which are polyfunctional towards amino groups; a process for their manufacture and their use as agents for increasing the retention of fibres, fillers and pigments
Complete information for OAZ1 gene (Protein Coding), Ornithine Decarboxylase Antizyme 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Tiburcio AF, Altabella T, Bitrián M and Alcázar R. (2014) The Roles of Polyamines during the lifespan of plants:from development to stress. Planta, doi: 10.1007/s00425-014-2055-9. online first March 2014 read here Alcazar, R.; Tiburcio, A. F. (2014) Polyamines in stress protection - applications in agriculture: Plant adaptation to environmental change: significance of amino acids and their derivatives Pages: 129-140. CABI; Wallingford; UK. ISBN: 978-1-78064-273-4. read 2013 ...
Polyamines are small, ubiquitous organic polycations that have been implicated in a wide variety of physiological functions including protein translation, membrane stabilization and cell proliferation (reviewed in Tabor and Tabor, 1984). As one might expect, biosynthesis of polyamines is essential for viability of both prokaryotic and eukaryotic cells. The most common polyamines are putrescine, spermidine and spermine, which contain two, three or four charged amine groups, respectively. Eukaryotic organisms contain all three of these amines at abundant levels (high micromolar to millimolar). Much attention has focused on the roles of polyamines in various disease processes. For instance, polyamines have been implicated in autoimmune disorders such as systemic lupus erythematosus (Brooks, 1994). Polyamines also accumulate in cancer cells, and high levels of polyamines are found in the urine from cancer patients (reviewed in Russel and Duri, 1978; Chanda and Ganguly, 1988). Increased levels of ...
An increasing body of evidence indicates that cellular polyamines play a critical role in maintenance of the intestinal epithelial integrity, but few specific functions of polyamines at cellular and molecular levels are defined. We (18) have recently reported that polyamines are implicated in regulation of the intestinal epithelial barrier function and that depletion of cellular polyamines increases epithelial paracellular permeability at least partially by inhibiting expression of adherens junctions in undifferentiated parental IEC-6 cells. The present studies further confirm our previous observations by demonstrating that polyamines are crucial for expression of adherens junctions in differentiated IEC-Cdx2L1 cells. The most significant of the new findings reported in this study, however, is that polyamines are required for normal function of tight junctions and that polyamines regulate expression of various tight junction proteins through different mechanisms. Polyamines regulate occludin ...
Polyamine transport actions have already been described in diverse multicellular systems, but their bioenergetic systems and molecular identification stay unclear. methylglyoxal bis(guanylhydrazone), NMDG, S2 (Schneider range 2) cells, the initial such activity referred to within a model hereditary system, and weve characterized its kinetics, ionic requirements and pharmacological profile. This activity displays particular affinity for spermine and spermidine, however, not putrescine, would depend on H+, however, not Na+, and displays a pharmacological profile strikingly identical to that from the Slc22 (solute carrier 22) category of solute companies. These data will facilitate additional tests for the molecular id and characterization from the spermine/spermidine transporter in as well as perhaps in various other species aswell. MATERIALS AND Strategies Reagents [14C]Spermine tetrahydrochloride (113?Ci/mol) and [14C]spermdine trihydrochoride (112?Ci/mol) had been extracted from Amersham ...
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Looking for Hexamine? Find out information about Hexamine. hexamine, or urotropine, colorless crystals with a sweet taste that carbonize at 280° C and sublime in vacuum above 230° C. Hexamethylenetetramine is highly... Explanation of Hexamine
PROBLEM TO BE SOLVED: To provide a polyamine useful as a diamine component of a polymer and a method for efficiently and inexpensively obtaining the polyamine. SOLUTION: The alicyclic polyamines are represented by the following formula (1): (Wherein, Y 1 represents a saturated aliphatic hydrocarbon group, and R 1 to R 4 is the same or different and represents a hydrogen atom, an alkyl group, a cycloalkyl group, an aryl group, or an aralkyl group). Alicyclic polyamines can be obtained by reductive amination of 3-formylcycloalkanones or 3-formylcycloalkenones.
A stable isotope labelling assisted LC-MS method was developed for highly sensitive quantitation of polyamines in roots and leaves of rice plants under cadmium stress. Successful quantitation of polyamines in minute amounts of plant extracts allowed the detection of spatial and temporal changes in an organ/t
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One of the recently developed polyamine (PA) analogues, N (1) ,N (11)-diethylnorspermine (DENSpm), has been found to act as an apoptotic inducer in melanoma, breast, prostate and colon cancer cells. Also, its potential to ...
phdthesis{8467fc6f-8c20-4634-9e13-8c10c0b1c8ef, abstract = {Our research group has previously shown that treatment with the polyamine biosynthesis inhibitors a-difluoromethylornithine (DFMO) or amidinoindan-1-one 2´-amidinohydrazone (CGP 48664) inhibited S phase progression before any other cell cycle phase was affected. This study was undertaken to further investigate the role of polyamines in the regulation of S phase progression and DNA synthesis. I have found that treatment with the polyamine analog <i>N</i><sup>1</sup>,<i>N</i><sup>11</sup>-diethylnorspermine (DENSPM) also caused a prolongation of the S phase. The common denominator for DFMO, CGP 48664, or DENSPM treatment is a depletion of the cellular spermidine pool. CGP 48664 and DENSPM in addition deplete the spermine pool. CGP 48664 or DENSPM treatment prolonged the S phase more than did DFMO treatment. Thus, mainly spermine but also spermidine may have a function in S phase ...
Polyamines are essential organic polycations which have been implicated in various cellular processes. Cellular polyamine concentrations are regulated mainly at the level of synthesis but also at the level of catabolism and export. Elevated cellular polyamine levels lead to the induction of antizyme translation by a highly conserved mechanism involving +1 ribosomal frameshifting. The antizyme targets ornithine decarboxylase (ODC), the rate limiting enzyme in polyamine biosynthesis for ubiquitin-independent degradation by the 26S proteasome. The N-terminal degron of ODC and the antizyme are essential for the effective degradation of ODC. Polyamines also regulate antizyme levels by blocking its ubiquitin-dependent protein degradation. In this study, the mechanisms of regulating polyamine levels by the antizyme are dissected ...
A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and their related monoacetyl derivatives in stomach tissue. The method is based on HPLC using octane sulfonate as an ion-pairing agent employed in acetate buffers at pH 4.5. The application is accompanied with fluorescence detection followed by post-column derivatization with o-phthaldialdehyde at room temperature (20±0.5°C). N1- and N8-acetylspermidines (ASPD) can be determined with this method in the same run without performing any special procedures or pre-purification in concentrations exceeding 8.5 pmoles. The variability in reproducibility of the day-today precision and duplicate determination, and simultaneous determination of standard mixture and biological samples were found < 2%. The mean (± s.e.mean) retention times (n=12) for putrescine (Put), N1-ASPD, N8-ASPD, spermidine (Spd) and spermine (Spm) are 8.97±0.025; 17.64±0.063; 18.99±0.133; 28.20±0.070 and 39.81±0
Polyamines are small cationic molecules that in adult brain are connected to neuronal signaling by regulating inward-rectifier K+-channels and different glutamate receptors. Antizyme inhibitors (AZINs) regulate the cellular uptake of polyamines and activate ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine synthesis. Elevated levels of ODC activity and polyamines are detected in various brain disorders including stroke and Alzheimers disease (AD).. We originally reported a novel brain- and testis-specific AZIN, called AZIN2, the distribution of which we have now studied in normal and diseased human brain by in situ hybridization and immunohistochemistry. We found the highest accumulation of AZIN2 in a pearl-on-the-string-like distribution along the axons in both the white and gray matter. AZIN2 was also detected in a vesicle-like distribution in the somas of selected cortical pyramidal neurons. Double-immunofluorescence staining revealed co-localization of AZIN2 and N-methyl ...
Next-day shipping cDNA ORF clones derived from OAZ2 ornithine decarboxylase antizyme 2 available at GenScript, starting from $99.00.
Viruses are suspected of significant roles in autoimmune diseases but the mechanisms are unclear. We get some insight by considering demands a virus places on host cells. Viruses require production of their own proteins, RNA and/or DNA, but also production of additional cellular machinery, such as ribosomes, to handle the increased demands. Since the nucleolus is a major site of RNA processing and ribonucleoprotein assembly, nucleoli are targeted by viruses, directly when viral RNA and proteins enter the nucleolus and indirectly when viruses induce increased expression of cellular polyamine genes. Polyamines are at high levels in nucleoli to assist in RNA folding. The size and activity of nucleoli increase directly with increases in polyamines. Nucleolar expansion due to abnormal increases in polyamines could disrupt nearby chromatin, such as the inactive X chromosome, leading to expression of previously sequestered DNA. Sudden expression of a large concentration of Alu elements from the disrupted
Increased polyamine biosynthesis activity and an active polyamine transport system are characteristics of many cancer cell lines and polyamine depletion has been shown to be a viable anticancer strategy. Polyamine levels can be depleted by difluoromethylornithine (DFMO), an inhibitor of the key polyamine biosynthesis enzyme ornithine decarboxylase (ODC). However, malignant cells frequently circumvent DFMO therapy by up-regulating polyamine import. Therefore, there is a need to develop compounds that inhibit polyamine transport. Collectively, DFMO and a polyamine transport inhibitor (PTI) provide the basis for a combination therapy leading to effective intracellular polyamine depletion. We have previously shown that the pattern of uptake of a series of polyamine analogues in a Drosophila model epithelium shares many characteristics with mammalian cells, indicating a high degree of similarity between the mammalian and Drosophila polyamine transport systems. In this report, we focused on the utility of the
TY - JOUR. T1 - Polyamines are needed for the differentiation of 3T3-L1 fibroblasts into adipose cells. AU - Bethell, Delia R.. AU - Pegg, Anthony E.. PY - 1981/9/16. Y1 - 1981/9/16. N2 - When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of α-difluoromethylornithine, an inhibitor of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation. The inhibitory effect of α-difluoromethylorinithine was completely abolished by provision of spermidine or putrescine. This suggests that polyamines are needed in the processes of differentiation as well as their established requirement for cell growth.. AB - When non-proliferating 3T3-L1 fibroblasts were stimulated to differentiate into adipose cells, there was a dramatic increase in the intracellular level of the polyamine, spermidine. Addition of ...
Polyamines are ubiquitous components of all eukaryotic cells and are found in brain tissue. A number of studies have shown that changes in the levels of polyamines occur following cerebral ischaemia. However, whether polyamines are neurotoxic or neuroprotective in ischaemia still remains unclear, although most of the evidence points to a toxic effect. The polyamine/NMDA antagonist N1-dansyl-spermine has been shown to be neuroprotective in a global ischaemia model. However, at the onset of the present study, the neuroprotective potential of this compound was not known in using focal ischaemia models, which are of more clinical relevance. Very recently, more novel polyamine analogues (BU31b, BU37b, BU33b, BU36b and BU43b) have been synthesized. These compounds, with the exception of BU37b, have shown some polyamine antagonist potential in vivo. Therefore these polyamine analogues are candidate neuroprotective agents. The pre-ischaemic effect of all these compounds mentioned above was investigated ...
TY - JOUR. T1 - Inactivation of IkB contributes to transcriptional activation of spermidine/spermine N(1)-acetyltransferase. AU - Choi, W.. AU - Proctor, L.. AU - Xia, Q.. AU - Feng, Y.. AU - Gerner, E. W.. AU - Chiao, P. J.. AU - Hamilton, S. R.. AU - Zhang, W.. N1 - Contribution: organisation=sgn,FACT1=1. PY - 2006. Y1 - 2006. U2 - 10.1002/mc.20239. DO - 10.1002/mc.20239. M3 - Article. VL - 45. SP - 685. EP - 693. JO - Molecular Carcinogenesis. JF - Molecular Carcinogenesis. SN - 0899-1987. IS - 9. ER - ...
Polyamines are nitrogenous, growth regulator like compounds, which are present in all living organisms. In plants, polyamines affect, for example, cell division, differentiation and stress tolerance. The project is coordinated by Metla and funded by the Academy of Finland. In laboratory experiments the roots of young seedlings of Scots pine were inoculated with the mycelium of S. variegatus and the development of the mycorrhizal interaction and changes in polyamine concentrations were monitored for five weeks. In the presence of the mycorrhizal fungus, the gene essential for the formation of polyamines was activated in wide areas of the developing root vascular tissue of the young Scots pine seedlings. The results show that the fungus induces the synthesis of plant polyamines and that polyamines play an important role in the formation of the symbiosis between S. variegatus and Scots pine seedlings. Mycorrhizal fungi are able to produce different amounts of polyamines. The results encourage ...
Polyamines in stress tolerance. Polyamines are small aliphatic compounds present in all living organisms. Polyamine biosynthesis is an evolutionary conserved pathway under an apparent purifying selection. Even though their essential role in stress tolerance is genetically demonstrated, molecular and signalling mechanisms underlying cell protection are relatively obscure. I am interested in gaining insight into the role of polyamines in stress tolerance and signalling (including developmental pathways) using advanced genetic approaches in plant science and exploring direct applications of polyamine content regulation (by means of natural variation) in crop breeding ...
Are milk polyamines preventive agents against food allergy? - Volume 59 Issue 1 - Guy Dandrifosse, O. Peulen, N. El Khefif, P. Deloyer, A. C. Dandrifosse, Ch. Grandfils
HEXAMINE Hexamine mainly used as curing agent for plastic and resin, catalytic agent and foaming agent for amino plastic, rubber vulcanization
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Approximately 18-45 patients with Hepatocellular Carcinoma (HCC) will be treated with DENSPM at approximately 5 centers in the United States. First, we
TY - JOUR. T1 - Growth arrest- and polyamine-dependent expression of spermidine/spermine N1-acetyltransferase in human tumor cells. AU - Ignatenko, Natalia. AU - Gerner, Eugene W.. PY - 1996/4. Y1 - 1996/4. N2 - Polyamines are essential for optimal cell growth. The regulation of polyamine biosynthetic, but not catabolic, enzymes has been studied in detail. Because intracellular polyamine contents depend on both synthesis and catabolism, we studied the regulation of spermidine/spermine N1- acetyltransferase (N1SSAT), the first enzyme in polyamine catabolism. Steady-state RNA levels of N1SSAT increased 3-5 fold as human colon tumor- derived HCT116 cells traversed the log phase and entered the plateau phase. Depletion of cellular polyamines, using α-difluoromethylornithine, caused a decrease in the steady-state levels of both the 1.3-kb N1SSAT transcript and its 3.5-kb precursor, without affecting the stability of either RNA. N1SSAT enzyme activity was low in cells with normal polyamine contents ...
Inhibition of polyamine biosynthesis and/or the perturbation of polyamine functionality have been exploited with success against parasitic diseases such as Trypanosoma infections. However, when the classical polyamine biosynthesis inhibitor, α-difluoromethylornithine, is used against the human malaria parasite, Plasmodium falciparum, it results in only a cytostatic growth arrest. Polyamine metabolism in this parasite has unique properties not shared by any other organism. These include the bifunctional arrangement of the catalytic decarboxylases and an apparent absence of the typical polyamine interconversion pathways implying different mechanisms for the regulation of polyamine homeostasis that includes the uptake of exogenous polyamines at least in vitro. These properties make polyamine metabolism an enticing drug target in P. falciparum provided that the physiological and functional consequences of polyamine metabolism perturbation are understood. This review highlights our current ...
Maintenance of intestinal mucosal epithelial integrity requires cellular polyamines that regulate expression of various genes involved in cell proliferation, growth arrest and apoptosis. Our previous studies have shown that polyamines are essential for expression of the c-myc gene and that polyamine-induced c-Myc plays a critical role in stimulation of normal IEC (intestinal epithelial cell) proliferation, but the exact downstream targets of induced c-Myc are still unclear. The p21Cip1 protein is a major player in cell cycle control, which is primarily regulated at the transcriptional level. The current study was designed to determine whether induced c-Myc stimulates normal IEC proliferation by repressing p21Cip1 transcription following up-regulation of polyamines. Overexpression of the ODC (ornithine decarboxylase) gene increased levels of cellular polyamines, induced c-Myc expression and inhibited p21Cip1 transcription, as indicated by repression of p21Cip1 promoter activity and a decrease in ...
Bcl-2 protein has been contributed with number of genes which are involved in oncogenesis. Among the many targets of Bcl-2, NF kappa B have potential role in induction of cell cycle arrest. Curcumin has potential therapeutic effects against breast cancer through multiple signaling pathways. In this study, we investigated the role of curcumin in induction of cell cycle arrest via regulating of NF kappa B and polyamine biosynthesis in wt and Bcl-2+ MCF-7 cells. To examine the effect of curcumin on cell cycle regulatory proteins, PI3K/Akt, NF kappa B pathways and polyamine catabolism, we performed immunoblotting assay. In addition, cell cycle analysis was performed by flow cytometry. The results indicated that curcumin induced cell cycle arrest at G2/M phase by downregulation of cyclin B1 and Cdc2 and inhibited colony formation in MCF-7 wt cells. However, Bcl-2 overexpression prevented the inhibition of cell cycle associated proteins after curcumin treatment. The combination of LY294002, PI3K ...
ODC1 [ENSP00000234111]. Ornithine decarboxylase 1; Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis; Belongs to the Orn/Lys/Arg decarboxylase class-II family.. Synonyms: ODC1, ODC1p, hODC1, C9JG30, P11926 .... Linkouts: STRING Pharos UniProt OMIM ...
ODC1 [ENSP00000234111]. Ornithine decarboxylase 1; Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis; Belongs to the Orn/Lys/Arg decarboxylase class-II family.. Synonyms: ODC1, ODC1p, hODC1, C9JG30, P11926 .... Linkouts: STRING Pharos UniProt OMIM ...
A number of polyamine analogs are currently used in various clinical trials as cancer treatment and it is important to investigate their effects not only on cancer cells but also on normal cells. Treatment with polyamine analogs depletes cells of polyamines and inhibits cell proliferation, but the analogs cannot take over the normal function of the natural polyamines in the cell. In this study, the normal-like breast epithelial cell line MCF-10A was treated with the polyamine analog N,N -diethylnorspermine (DENSPM). The cells were then studied using a bromodeoxyuridine-DNA flow cytometry method as well as western blot. The ability of both normal-like and breast cancer cells to recover from DENSPM treatment was also studied. DENSPM treatment of MCF-10A cells resulted in a prolongation of the S and G(2) + M phases, followed by a G(1)/S block. The p53/p21/RB1 pathway was involved in the G(1)/S block as shown by increased levels of p53 and p21 detected by western blot. Decreased levels of cyclin ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Previous studies have shown that amine groups are ototoxic. The interaction between different polyamines and phospholipid vesicles was studied using vesicle aggregation and fluorescence techniques (DPH and ANS as the fluorescence probes). The results showed that the interaction between polyamines (spermine, spermidine and 1,3-diaminopropane) and acidic phospholipids (PS, PE, PI and PIP2) is an ionic one. The polyamine with the highest positive charges and the phospholipid with the highest content of negative groups showed the strongest ionic interaction. There was no indication of any hydrophobic interaction within the phospholipid bilayer. The strong interaction between amine groups and PIP2 support the proposal that the latter is crucially involved in aminoglycoside toxicity in the inner ear and kidney.
F14512, a Potent Antitumor Agent Targeting Topoisomerase II Vectored into Cancer Cells via the Polyamine Transport System Jean-Marc Barret, Anna Kruczynski,
Two genes,LAT1andOCT1, are likely to be involved in polyamine transport in Arabidopsis. Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine. Arabidopsis sp
Dont worry about hexamine cobalt chloride (Im assuming that you are doing some sort of 5-RACE). I did the type of ligation you are talking about recently using only the 10xbuffer provided with the buffer (50 mM Tris-HCl (pH 7.8), 10 mM MgCl2, 10 mM DTT and 1 mM ATP). I precipitated the oligo and ssDNA together and performed the ligation reaction in a minimal volume over night at 37C using 40 units of RNA ligase. It worked fine. But in other application I dont know whether hexamine cobalt chloride makes any difference... Marieluise Kirchner wrote: , used anyone the T4 RNA ligase for ssDNA / oligonucleotide-ligation? in , the buffer of my protocoll hexamine cobalt chloride is used. Why? Is the , effect really that big? Has hexamine cobalt chloride any bad effects in , further reactions like PCR or ligation into a vector? , The question is - should i use this hexamine cobalt chloride or better , not? , , thanks and a nice evening , , marie , , --- ...
Die Universität zu Köln ist eine Exzellenzuniversität mit dem klassischen Fächerspektrum einer Volluniversität. Als eine der größen Hochschulen Europas arbeitet sie in Forschung und Lehre auch international auf höchstem Niveau.
Effect of nor-NOHA on arginase activity and amino acid levels. (A) Significant arginase inhibition was observed in cell lysates of CL-19 cultures treated with n
Author: Puleston, Daniel J. et al.; Genre: Journal Article; Published in Print: 2019; Title: Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation
Klempan TA, Rujescu D, Mérette C, Himmelman C, Sequeira A, Canetti L, et al. Profiling brain expression of the spermidine/spermine N1-acetyltransferase 1 (SAT1) gene in suicide. Am J Med Genet B Neuropsychiatr Genet. 2009;150B(7):934-43. ...
Klempan TA, Rujescu D, Mérette C, Himmelman C, Sequeira A, Canetti L, et al. Profiling brain expression of the spermidine/spermine N1-acetyltransferase 1 (SAT1) gene in suicide. Am J Med Genet B Neuropsychiatr Genet. 2009;150B(7):934-43. ...
SAT1 - SAT1 (untagged)-Human spermidine/spermine N1-acetyltransferase 1 (SAT1), transcript variant 1 available for purchase from OriGene - Your Gene Company.
The objective of this project was to synthesize a protected spermidine derivative, which is a polyamine derivative. The spermidine that was to be synthesised is a N8 protected spermidine derivative.
Increased arginase activity contributes to airway nitric oxide (NO) deficiency in cystic fibrosis (CF). Whether down-stream products of arginase activity contribute to CF lung disease is currently unknown. The objective of this study was to test whether L-ornithine derived polyamines are present in CF airways and contribute to airway pathophysiology. Polyamine concentrations were measured in sputum of patients with CF and in healthy controls, using liquid chromatography-tandem mass spectrometry. The effect of spermine on airway smooth muscle mechanical properties was assessed in bronchial segments of murine airways, using a wire myograph. Sputum polyamine concentrations in stable CF patients were similar to healthy controls for putrescine and spermidine but significantly higher for spermine. Pulmonary exacerbations were associated with an increase in sputum and spermine levels. Treatment for pulmonary exacerbations resulted in decreases in arginase activity, L-ornithine and spermine concentrations in
The current study shows that topically applied dl-α- tocopherol reduced polyamine content in the skin of patients with large numbers of established AKs on the forearms. Elevated polyamine levels are characteristically seen in many types of neoplastic cells and tissues. OBrien and colleagues (29) and Peralta Soler and colleagues (30) have shown that in transgenic mice, overexpression of ornithine decarboxylase in skin leads to changes in tissue polyamine levels, particularly putrescine, and can modulate the development and maintenance of the neoplastic phenotype. Joshi (31) further hypothesized that polyamine synthesis is expected to promote endothelial cell proliferation and therefore angiogenesis. This reinforces the known role of polyamine biosynthesis in mediating skin tumor initiation and promotion (29-33).. The main findings of the present study can be summarized by the following statements. Daily topical application resulted in substantial uptake of free dl-α-tocopherol into the forearm ...
TY - JOUR. T1 - Stage-specific polyamine metabolism in Trypanosoma cruzi. AU - Bacchi, Cyrus J.. AU - Braunstein, Vicki L.. AU - Rattendi, Donna. AU - Yarlett, Nigel. AU - Wittner, Murray. AU - Tanowitz, Herbert B.. PY - 2001. Y1 - 2001. UR - UR - U2 - 10.1111/j.1550-7408.2001.tb00519.x. DO - 10.1111/j.1550-7408.2001.tb00519.x. M3 - Article. C2 - 11906064. AN - SCOPUS:0035544896. VL - 48. SP - 201S-202S. JO - Journal of Eukaryotic Microbiology. JF - Journal of Eukaryotic Microbiology. SN - 1066-5234. IS - SUPPL.. ER - ...
Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
The work in this thesis has covered three main topics; i) cytotoxic polyamine-conjugates ii) radiation protection polyamine-conjugates iii) polyamine conjugates which probe cellular uptake and DNA binding. The synthesis of these conjugates employed selective protection/deprotection steps, taking advantage of the BOC protecting groups regioselectivity of primary over secondary amines when reacting with naturally occurring polyamines.;After promising in vitro and in vivo results of the original spermidine-chlorambucil synthesised by Wheelhouse (1990), attempts were made to improve this compound. The terminal amines were ethylated. The new diethyl derivative showed greater inhibition of [C14] labelled spermidine entry into cultured cells, and improved cytotoxicity. Also a new novel cis-platin polyamine conjugate was synthesised and tested for cellular inhibition of radiolabelled spermidine and cytotoxicity. It failed to recognise the polyamine uptake receptor and gave poor [C14] spermidine ...
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Get a sample of the report, please email to:[email protected] SummaryThe Global And China Polyamide Polyamine Epichlorhydrin (PPE) Sales 2017...
Barber, D. J.; Scott, E. R. D. (14 May 2002). "Origin of supposedly biogenic magnetite in the Martian meteorite Allan Hills ... "KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis". Nature Communications. 6: ... Biomagnetism is usually related to the presence of biogenic crystals of magnetite, which occur widely in organisms. These ... Hence, the presence of trace levels of biogenic magnetite in virtually all human tissues examined suggests that similar ...
Sarcosine; Polyamine site agonists: Neomycin. *Spermidine. *Spermine; Other positive allosteric modulators: 24S- ... Biogenic amines: Monoamines: 6-OHM. *Dopamine. *Epinephrine (adrenaline). *NAS (normelatonin). *Norepinephrine (noradrenaline) ...
Sarcosine; Polyamine site agonists: Neomycin. *Spermidine. *Spermine; Other positive allosteric modulators: 24S- ... Biogenic amines. Monoamines. *6-OHM. *Dopamine. *Epinephrine (adrenaline). *NAS (normelatonin). *Norepinephrine (noradrenaline) ...
Several polynuclear Pt(II) chelates with biogenic polyamines were synthesized and screened for their potential ... Cytotoxic Activity of Metal Complexes of Biogenic Polyamines: Polynuclear Platinum(II) Chelates. por. ...
Polyamines and biogenic amines are chemicals derived from amino acids. Polyamines are involved in intracellular signals. ... Green Tea, Polyamines and Biogenic Amines.. Posted on January 14, 2014. by Dr. Gary Pack ... Epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) targets the pathways of biogenic amines and polyamines. ... Miscellaneous: Biogenic amines, Caffeine, Cyclooxygenase, Dopamine, l-DOPA, Lipoxygenase inhibitor, Methylxanthine, ...
Polyamines and biogenic amines are chemicals derived from amino acids. Polyamines are involved in intracellular signals. ... Green Tea, Polyamines and Biogenic Amines.. Posted on January 14, 2014. by Dr. Gary Pack ... Epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) targets the pathways of biogenic amines and polyamines. ... Miscellaneous: Biogenic amines, Caffeine, Cyclooxygenase, Dopamine, l-DOPA, Lipoxygenase inhibitor, Methylxanthine, ...
Given that polyamines can interact with many molecules, it is not surprising that they affect aging via several mechanisms. ... Molecular basis of the anti-aging effect of spermidine and other natural polyamines - a mini-review Gerontology. 2014;60(4): ... Objectives: An up-to-date overview of the so far identified mechanisms of action of spermidine and other polyamines on aging is ... Methods: Studies of aging and of the molecular effects of polyamines in general and spermidine in particular are used to ...
A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth ... Spermine is a polyamine, a small organic cations that is absolutely required for eukaryotic cell growth. Spermine, is normally ... Spermine is a polyamine. It is an organic molecule that is involved in cellular metabolism. ...
The role of biogenic polyamines. Nucleic Acids Res 27:3931-3937PubMedCrossRefGoogle Scholar ...
Biogenic Polyamines / analysis * DNA, Bacterial / genetics * DNA, Ribosomal / genetics * Fatty Acids / analysis ...
... coli polyamine biosynthesis pathway. C. Analysis of polyamine content in E. coli polyamine mutants grown on antibiotic-free ... Exposure to biogenic ammonia increases the intracellular pool of E. coli polyamines. A. Effect of supplementation of LB agar ... Here we show that bacterial uptake and metabolism of biogenic ammonia increases the intracellular level of polyamines and ... the use of combined multiple mutations of polyamine synthesis genes demonstrated that the reduction or absence of polyamines ( ...
... and polyamine. It is clear that CD spectroscopy is extremely sensitive and relatively inexpensive, as compared with other ... DNA interaction with antitumor polyamine analogues: A comparison with biogenic polyamines. Biomacromolecules 2008, 9, 2712-2718 ... Ouameur, A.A.; Bourassa, P.; Tajmir-Riahi, H.A. Probing tRNA interaction with biogenic polyamines. RNA 2010, 16, 1968-1979. [ ... Ouameur, A.A.; Tajmir-Riahi, H.A. Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine ...
Biogenic amines (BA) are potentially unsafe nitrogenous compounds that result from the decarboxylation of some amino acids. ... Biogenic amines (BA) are potentially unsafe nitrogenous compounds that result from the decarboxylation of some amino acids. ... On the other hand, Staphylococcus xylosus and Debaryomyces hansenii strains have been reported to degrade biogenic amines in ... particularly biogenic amines, nitrosamines, polycyclic aromatic hydrocarbons (PAH), and mycotoxins. ...
Biogenic Polyamines without derivatization *Analytical. Related Product:Triart PFP Data No. F130703B_polyamines 2013/10/03 ...
Information on gastric tissue polyamines and their acetylated derivatives may be useful in understanding the mechanism of drugs ... A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and ... A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and ... Keywords: Endogenous polyamines; HPLC; Post-column derivatization; Rat; Gastric tissue Endogenous polyamines; HPLC; Post-column ...
Biogenic polyamines spermine and spermidine participate in numerous cellular processes including transcription, RNA processing ... homogenate on polyamine biosynthesis and polyamine degradation as well as on resulting polyamine levels in HGC-27 human gastric ... METHODS: Dietary polyamine data were available for 188 of 267 patients completing the study. Total dietary polyamine content ... Polyamines are synthesised endogenously and obtained from dietary sources. Here we investigate dietary polyamine intake and ...
Putrescine is a biogenic polyamine and precursor of spermidine. It is formed via decarboxylation of ornithine or by ... Spermidine is biogenic polyamine formed from putrescine, a precursor of spermine. Spermidine has a role in cell growth ... Binds to the polyamine modulatory site of the NMDA receptor and potentiates NMDA-induced currents; precursor of spermidine. ...
Ramirez, F. J.; Thomas, T. J.; Antony, T.; Ruiz-Chica, J.; Thomas, T.: Effects of aminooxy analogues of biogenic polyamines on ... Antony, T.; Hoyer, W.; Cherny, D. I.; Heim, G.; Jovin, T. M.; Subramaniam, V.: Cellular polyamines promote the aggregation of α ... Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force ...
Ramirez, F. J.; Thomas, T. J.; Antony, T.; Ruiz-Chica, J.; Thomas, T.: Effects of aminooxy analogues of biogenic polyamines on ...
Atmospheric CO_2 captured by biogenic polyamines is transferred as a possible substrate to Rubisco for the carboxylation ...
Certain endogenous biogenic D,L-amino acids, polyamines, and advanced glycation end-products (AGEs) have been detected in ... ...
Biogenic polyamines promote the aggregation of α‐synuclein and may constitute endogenous agents modulating the pathogenesis of ... A) No polyamine (0), and 100 μM putrescine (1), spermidine (2), spermine (3) and the synthetic polyamines 4‐4‐4 (4) and BE‐4‐4‐ ... A) The different polyamines are designated as in Figure 1. Solid lines, global fits (for each polyamine, assigning the same ... ii) a constant fraction, β, of monomer (free or aggregated) in a polyamine‐liganded form, dictated by polyamine type and ...
Structural analysis of DNA interactions with biogenic polyamines and cobalt(III)hexamine studied by Fourier transform infrared ... Effect of polyamines on mitochondrial F-ATPase from Crithidia fasciculata and Trypanosoma cruzi. ...
E. Standarová, L. Vorlová, and L. Gallas, "The distribution of biogenic amines and polyamines in pheasant meat," Maso ...
It can also be used for separation of moderately hydrophobic amines, including biogenic amines, alkyldiamines, and polyamines. ... For polyvalent and moderately hydrophobic amines, including diamines and biogenic amines *Optimized for simple acidic gradient ... including biogenic amines and diamines, using simple aqueous eluents and elevated temperature (40 °C). The Dionex IonPac CS17 ... including biogenic amines and diamines, using simple aqueous eluents and elevated temperature (40 °C). The Dionex IonPac CS17 ...
The main mechanism of biogenic formation is the decarboxylation of free amino acids by specific enzymes of microbial origin, ... the production of biogenic amines. The physiological effects of tyramine include peripheral vasoconstriction, increased cardiac ... This chapter discusses the formation and degradation of biogenic amines, their occurrence in foods, their significance in food ... Kalac, P.,, and M. Krizek. 2003. A review of biogenic amines and polyamines in beer. J. Inst. Brew. 109:123-128.. ...
In particular photosensitive polyamines have been recently used to photo-trigger the hybridization/melting of DNA as well as to ... Direct comparison of our results achieved by using photosensitive polyamines with those previously reported for biogenic and ... In particular photosensitive polyamines have been recently used to photo-trigger the hybridization/melting of DNA as well as to ... Probing the binding mechanism of photoresponsive azobenzene polyamine derivatives with human serum albumin M. Deiana, Z. ...
This odor has been associated with increases in several biogenic amines (BAs) that may serve as important biomarkers. Within ... This odor has been associated with increases in several biogenic amines (BAs) that may serve as important biomarkers. Within ... One clinical feature of BV, malodor, has been linked to increases in vaginal biogenic amines (BAs), including the polyamines ... Determining Biogenic Amine Levels Corresponding to CSTs. Sample Set for Measuring Biogenic Amine Levels. Analyses were ...
Biogenic Amines;Foods of Animal Origin;Korean Domestic Markets; ... Quantitative Analysis of Biogenic Amines in Raw and Processed ... Biogenic amine and polyamine contents in meat and meat products. J. Agric. Food Chem. 45:2098-2102. ... Distribution of biogenic amines and polyamines in cheese. J. Food Sci. 68(3):750-755. ... Quantitative Analysis of Biogenic Amines in Raw and Processed Foods of Animal Origin on Korean Domestic Market. Min, Joong-Seok ...
O. A. Smirnova, T. A. Keinanen, O. N. Ivanova et al., "Hepatitis C virus alters metabolism of biogenic polyamines by affecting ... Polyamine Catabolism. The natural polyamines (PAs), putrescine, spermidine, and spermine, are involved in multiple basic ... "Cloning and characterization of a human polyamine oxidase that is inducible by polyamine analogue exposure," Cancer Research, ... N. Seiler, "Polyamine metabolism," Digestion, vol. 46, no. 2, pp. 319-330, 1990. View at: Publisher Site , Google Scholar*A. E ...
Biogenic Amines;Volatile Basic Nitrogen;Beef;Pork;Chicken; ... Relationship between the Concentration of Biogenic Amines and ... Biogenic amine and polyamine contents in meat and meat products. J. Agric. Food Chem. 45:2098-2102. ... Polyamines in food and their consequences for food quality and human health. Trends in Food Sci. Technol. 6:341-346. ... Determination of biogenic amines in fresh unpacked and vacuum-packed beef during storage at 4. $^{\circ}$. C. Food Chem. 74:515 ...
Putrescine is a biogenic polyamine naturally produced in peri-ovulatory ovaries in all animal species studied thus far. Peri- ...
Polar amines (alkanolamines and methylamines) and moderately hydrophobic amines (biogenic amines, diamines and polyamines) in ... Moderately low-capacity cation-exchange column for analysis of aliphatic amines, aromatic amines, and polyamines using MSA or ... Gradient separation of polyvalent and more hydrophobic amines, biogenic amines, and diamines. This newer column replaces the ... They are excellent for separations of alkali metals, alkaline earth metals, alkylamines, alkanolamines and biogenic amines. ...
  • Low-Capacity Cation-Exchange Column for Gradient Profiling and Amine Determination The Thermo Scientific Dionex IonPac* CS17 carboxylate-functionalized cation-exchange column is tailored for gradient separation of polyvalent and moderately hydrophobic amines, including biogenic amines and diamines, using simple aqueous eluents and elevated temperature (40 °C). The Dionex IonPac CS17 is an excellent column for use with Reagent-Free* IC systems using eluent generation, which requires only a deion. (
  • The Thermo Scientific Dionex IonPac* CS17 carboxylate-functionalized cation-exchange column is tailored for gradient separation of polyvalent and moderately hydrophobic amines, including biogenic amines and diamines, using simple aqueous eluents and elevated temperature (40 °C). The Dionex IonPac CS17 is an excellent column for use with Reagent-Free* IC systems using eluent generation, which requires only a deionized water source to produce methanesulfonic acid eluent. (
  • It can also be used for separation of moderately hydrophobic amines, including biogenic amines, alkyldiamines, and polyamines. (
  • Cytotoxic effects of metal complexes of biogenic polyamines. (
  • 18. Komprda T., Smělá D., Novická K., Kalhotka L., Šustová K., Pechová P.: Content and distribution of biogenic amines in Dutch-type hard cheese. (
  • A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and their related monoacetyl derivatives in stomach tissue. (
  • This chapter discusses the formation and degradation of biogenic amines, their occurrence in foods, their significance in food safety, their potential use as quality indicators, and the available methods for their determination. (
  • 1993. Liquid chromatographic determination of biogenic amines in dry sausages. (
  • 2000. Combined ion-pair extraction and gas chromatography - mass spectrometry for the simultaneous determination of diamines, polyamines and aromatic amines in Port wine and grape juice. (
  • 2001. Determination of biogenic amines in fresh unpacked and vacuum-packed beef during storage at 4 $^{\circ}$ C. Food Chem. (
  • Therefore, biogenic amines monitoring in food samples is a matter of utmost importance, and their accurate determination is considered indispensable. (
  • Under this context, we provide an overview over the most widely employed analytical techniques for biogenic amines determination such as chromatographic techniques and biosensors, emphasizing on new approaches. (
  • Biogenic amines share common characteristics with their precursors, AAs, and that is taken into consideration when we try to come up with analytical methods for their effective determination. (
  • Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC). (
  • 9. Durlu-Özkaya F., Ayhal K., Özkan G.: Biogenic amines determination in Tulum cheese by high performance liquid chromatography (HPLC). (
  • The present work describes the development and optimization of electrochemical biosensors for specific determination of the biogenic polyamine spermine (Spm) and spermidine. (
  • Changes in the concentrations of biogenic amines (BAs) in fresh beef, pork, and chicken breast and leg were investigated during storage, and the relationship between the content of volatile basic nitrogen (VBN) and BAs was evaluated. (
  • Biogenic amine concentrations in meat are associated with the proliferation of contaminating microorganisms. (
  • A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. (
  • Several studies show that cancer cell proliferation is dependent on polyamine levels and that there are higher concentrations in cancer cells compared to normal cells, or that there are relationships between polyamine metabolism and cancers. (
  • The polyamines Spd and spermine (Spm) are present at high concentrations in almost all cell types and are considered mediators of important cellular functions such as proliferation, differentiation, and tissue integrity ( Pegg, 2009 ). (
  • All diets comprised of cells of various types of living organisms and their associated substances thus contain polyamines, but in a wide variety of concentrations. (
  • The table that we created provides both the polyamine concentrations in various foods and important information for creating menus for the adjunctive treatment of various pathological conditions. (
  • Because foods are comprised of various organisms and their associated products, almost all foods contain polyamines, but in a wide variety concentrations. (
  • Polyamines in foods are thus one of the important sources of polyamines in the body and the longterm and continuous intake of a polyamine-rich diet increases the polyamine concentrations in whole blood [4,5]. (
  • And a continuous and long-term increased polyamine intake gradually elevated blood spermine levels in humans and mice, and lifelong consumption of polyamine rich chow of which polyamine concentrations were about three times higher than those of soybeans suppressed age-associated pathologies and extended the lifespan of mice [5,20]. (
  • In the present study, we created a table providing the polyamine concentrations of various foods on a calorie basis as well as on a weight basis, for a better understanding of the polyamine concentrations in food. (
  • Using Escherichia coli K12 as a model organism, and increased resistance to tetracycline as the phenotypic read-out, we demonstrate that exposure to ammonia generated by the catabolism of l -aspartate increases the level of intracellular polyamines, in turn leading to modifications in membrane permeability to different antibiotics as well as increased resistance to oxidative stress. (
  • ROS overproduction can result from changes in many processes, such as oxidative phosphorylation (OXPHOS), transition metal ions, oxidase activity, protein folding, thymidine, and polyamine catabolism [ 3 - 7 ]. (
  • Spermidine/spermine-N1-acetyltransferase (SSAT) is a highly inducible and tightly controlled enzyme that is the rate-limiting step for polyamine catabolism. (
  • The addition of preservatives to foods influences the microbial population dynamics and, consequently, the production of biogenic amines. (
  • A combined model of hepatic polyamine and sulfur amino acid metabolism to analyze S-adenosyl methionine availability. (
  • These results indicated that exogenous ASC influenced polyamine metabolism. (
  • Alcázar R, Cuevas JC, Patrón M, Altabella T, Tiburcio AF (2006) Abscisic acid modulates polyamine metabolism under water stress in Arabidopsis thaliana. (
  • The same visual stimulation protocol also induces a polyamine synthesis-dependent reduction in Ca2+-permeable AMPAR-mediated synaptic drive, suggesting that the increased excitability may compensate for this reduction. (
  • Accordingly, the change in excitability was prevented by blocking polyamine synthesis during visual stimulation and was rescued when Ca2+-permeable AMPAR-mediated transmission was selectively reduced. (
  • Deregulation of the level of polyamines and enzymes of their synthesis is often associated with pathological conditions, in particular, tumor growth, and their level in urine and plasma can be used as a diagnostic indicator of cancer. (
  • By allowing in particular to initiate the synthesis of DNA and proteins, polyamines are thus closely related to the growth processes of cells, including cancer cells. (
  • Alterations in synaptic transmission are implicated in brain dysfunction in PKU, and several experimental data suggest that the principal cause for this dysfunction is the impairment in biogenic amine synthesis (5). (
  • Inhibition of polyamine uptake potentiates the anti-proliferative effect of polyamine synthesis inhibition and preserves the contractile phenotype of vascular smooth muscle cells. (
  • Conversely, a restriction of polyamine intake with the inhibition of polyamine synthesis by suppressing the activities of enterobacteria by antimicrobial agents can decrease polyamine levels in the body [6,7]. (
  • In our complexes, the Pt(II) or Pd(II) ion is not bound to ammonia as in cisplatin but it is bound to NH 2 and NH groups of linear polyamines. (
  • Vibrational spectroscopy [both Raman and INS (inelastic neutron scattering)], coupled to quantum mechanical calculations, was used in order to perform a thorough structural analysis of linear polyamines and polynuclear polyamine metal chelates [e.g. with Pt(II) and Pd(II)] with potential anticancer activity. (
  • This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. (
  • DFMO is an irreversible inhibitor of ornithine decarboxilase (ODC), the key enzyme in mammalian polyamine biosynthesis, and has been shown to induce apoptosis. (
  • 1994. Biogenic amines and their production by microorganisms in food. (
  • The problem of biogenic amines in fermented foods and the use of potential biogenic amine-degrading microorganisms as a solution. (
  • 2012. Selection of biogenic amine-reducing microorganisms from a traditional Korean-style fermented food, Cheonggukjang. (
  • Biogenic amine formation and degradation by potential fish silage starter microorganisms. (
  • 1996. Biogenic amines in dry sausages as affected by starter culture and contaminant amine-positive lactobacillus. (
  • 1996. Biogenic amine sources in cooked cured shoulder pork. (
  • Erdei L, Szegletes Z, Barabas K, Pestenacz A (1996) Responses in polyamine under osmotic and salt stress in sorghum and maize seedlings. (
  • We have shown that the ability to enhance SSAT activity is a useful tool for cancer research, perhaps especially so for prostate cancer since the polyamine pathway is particularly active in this organ. (
  • Biogenic amines are low-molecular-mass substances, essential for proper health for all organisms. (
  • In the presence of spermine oxidase - the activities of which are observed in some pathological conditions such as Helicobacter pylori infection - increased polyamine intake (especially of spermine) may have deleterious effects on the stomach mucosa, because spermine oxidase degrades spermine to produce virulent substances such as aldehydes and hydrogen peroxides [19]. (
  • There were significant differences in the bacterial cell counts, pH value, titratable acidity, salinity, and biogenic amine content between the samples. (
  • Technological factors affecting biogenic amine content in foods: A review. (
  • 1. The effect of natural polyamines on KATP channels was studied using inside-out patches from guinea-pig ventricular myocytes. (
  • 6. Our results indicate that natural polyamines influence native KATP channel gating in cardiac myocytes. (
  • Biogenic amines are amino acid-derived compounds involved in the most important physiological functions: cell proliferation and differentiation, immunity, neurotransmission and neuroendocrine system, fertility. (
  • Polyamines are molecules of low molecular weight, consisting of a carbon chain having at least two amino groups positively charged at physiological pH. (
  • Monitoring of biogenic amines in cheeses manufactured at small-scale farms and in fermented dairy products in the Czech Republic. (
  • 13. El-Zahar K.M., El-Zaher A.M.A., Ramadan M.F.: Levels of biogenic amines in cheeses and their impact on biochemical and histological parameters in rats. (
  • In about 10% of the tested cheeses, the total concentration of all the monitored biogenic amines and polyamines exceeded the level of 200 mg/kg, which can be considered toxicologically significant. (
  • Bran and germs of cereals, vegetables, mushrooms, beans, and certain seafood's and aged cheeses were defined as polyamine- rich foods per calorie. (
  • Inhibition of Polyamine Formation Antagonizes Vascular Smooth Muscle Cell Proliferation and Preserves the Contractile Phenotype. (
  • Biogenic amines are known to have various biological functions such as not only neurotransmitter and cell proliferation but also food poisoning. (
  • Several polynuclear Pt(II) chelates with biogenic polyamines were synthesized and screened for their potential antiproliferative and cytotoxic activity in different human cancer cell lines. (
  • Condensing agents such as cobalt hexamine and biogenic polyamines such as spermidine, also crowding agents such as polyethylene glycol can be used to effectively increases the concentration of enzymes. (
  • The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. (
  • Contrarily, we showed that an increase in the polyamine concentration inhibited the age-associated or polyamine deficit-induced pro-inflammatory status and abnormal DNA methylation in mice. (
  • Here we review the recent CD spectroscopic studies of the induction of DNA conformational changes by different ligands, which includes metal derivative complex of aureolic family drugs, actinomycin D, neomycin, cisplatin, and polyamine. (
  • DB407 and B. subtilis DB821 showed probiotic properties including tolerance to artificial digestive juices, adherence to intestinal epithelial cells, resistance to antibiotics, and antibacterial activity against biogenic amine-producing strains. (
  • The origin of polyamines is primarily food and from the intestinal flora, although the body can also make. (
  • This experiment aimed to investigate the effects of liquid metabolite (LM) produced by Lactobacillus plantarum and dietary putrescine (PUT) on growth, blood lipids, villus height (VH), crypt depth (CD) and polyamines (PAs) content of intestinal tissue and ileal digesta in chickens. (
  • Duodenal PAs declined in the chickens fed LM, whereas dietary putrescine had no effects on intestinal tissue polyamine. (
  • LM influenced intestinal tissue PAs but had no effects on growth and ileal digesta polyamine content. (
  • Food polyamines are one of the important sources of body polyamines in mammals, as they have been found to be absorbed quickly from the intestinal lumen and distributed widely throughout the organs and tissues in the body. (
  • Polyamines in the intestinal lumen are absorbed quickly and distributed throughout all organs and tissues [1-3]. (
  • The values for putrescine, a precursor of polyamines containing two amine residues, were omitted for the following reasons:(1) Putrescine is degraded in the intestinal lumen, and only a small percentage is absorbed. (
  • Studies of aging and of the molecular effects of polyamines in general and spermidine in particular are used to synthesize our knowledge on what molecular mechanisms spermidine and other polyamines trigger to positively affect aging. (
  • Biogenic amines (BAs) are small molecular organic nitrogenous compounds (bases), polar or semipolar. (
  • Polyamines are low molecular weight molecules that cause strong electrostatic interaction with other molecules. (
  • In recent years, molecular aspects of polyamine action have also begun to emerge. (
  • R. Stevanato, M. Bertelle and S. Fabris, " In Vitro Preliminary Evidences on the Antioxidant Properties of Biogenic Amines," Pharmacology & Pharmacy , Vol. 4 No. 9, 2013, pp. 696-700. (
  • 1997. Biogenic amine and polyamine contents in meat and meat products. (
  • 2002) Biogenic amine formation in bottled beer. (
  • Therefore, changes to soil environment caused by agricultural management can affect SOM production, thereby modifying the route for biogenic and physicogenic aggregate formation (Pulleman 2002). (
  • Biogenic Amine Degradation by Bacillus Species Isolated from Traditional Fermented Soybean Food and Detection of Decarboxylase- Related Genes. (
  • Biogenic Amines in Foods, p 248-274. (
  • This study was performed to examine the levels of biogenic amines (BAs) in foods of animal origin such as egg, ham, sausage, milk, cheese and yoghurt distributed on Korean domestic markets, and to compare the results with those of western countries. (
  • Finally, we focus on foods in which biogenic amines mainly occur such as fish, meat and wine and other fermented products. (
  • Scientific opinion on risk based control of biogenic amine formation in fermented foods. (
  • In conclusion, the two probiotic Bacillus strains may be considered as the suitable starter for manufacture of fermented soybean foods with low biogenic amines content. (
  • 2006. Evaluation of biogenic amines in Korean commercial fermented foods. (
  • 3. Bodmer S., Imark C., Kneubuhl M.: Biogenic amines in foods. (
  • 15. Ercan S.S., Bozkurt H., Soysal C.: Significance of biogenic amines in foods and their reduction methods. (
  • The use of such a diet may, however, raise questions because of the exclusion of certain foods normally considered to be beneficial to health, but this is a specific protocol, the possible benefits of which may justify its temporary application without questioning the long-term interests of foods that are rich in polyamines. (
  • However, knowledge of the polyamine content in various foods is not complete. (
  • In the present study, based on published reports, we have created a visual table of the polyamine content in foods on a calorie basis. (
  • Biosilicas in diatom and marine sponge are formed under ambient conditions using organic matrices, unique proteins, and long-chain polyamines. (
  • Biogenic amines in fish: roles in intoxication, spoilage, and nitrosamine formation. (
  • The roles of food in the prevention and support of certain pathologies are more and more highlighted, the example of polyamines is significant. (
  • Differential and functional interactions emphasize the multiple roles of polyamines in plants. (
  • Several studies showed that polyamines have many biological activities, including roles in membrane stability, signal transduction, and gene expression, and the protection of cells and DNA from harmful stimuli such as radiation, ultraviolet, singlet oxygen, superoxide, and anticancer drugs [8-16]. (
  • These roles underline the importance of food polyamines in human health and diseases. (
  • Cellular polyamines promote the aggregation of α-synuclein. (
  • Thus, transcriptome, limited protein profiling, and metabolome studies of transgenic tomato fruit have yielded significant new information on cellular processes impacted by polyamine manipulation. (
  • Biogenic polyamines promote the aggregation of α‐synuclein and may constitute endogenous agents modulating the pathogenesis of PD. (
  • We show that the inability to import ammonia via the Amt gas channel or to synthesize polyamines prevent modification in the resistance profile of aerially exposed bacteria. (
  • In fermented dairy products, the tested biogenic amines occurred in relatively low amounts (generally up to 30 mg/kg) that are regarded safe for the consumer's health. (
  • Information on gastric tissue polyamines and their acetylated derivatives may be useful in understanding the mechanism of drugs or agents that play some part in gastric ulcer production or its repair mechanisms. (
  • These results prompted us to synthesize azobenzene-based polyamine derivatives and study their impact towards human serum albumin (HSA), the principal extracellular protein in plasma, which is highly responsible for the proper biological activity exerted by exogenous compounds. (
  • Herein, we show that both the mono-substituted Azo-4N and bi-substituted bis-Azo-4N azobenzene derivatives bind the protein template with moderate affinity and the number of positive charges along the polyamine moiety plays a pivotal role in stabilizing the photochrome-HSA adduct. (
  • Influence of the biogenic polyamines spermine, spermidine, and putrescine as well as their derivatives on the replication enzymes of hepatitis C virus (HCV) was investigated. (