Biofilms
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Plankton
Staphylococcus epidermidis
Microbial Viability
Pseudomonas aeruginosa
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Bacterial Adhesion
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Candida albicans
Streptococcus oralis
Microscopy, Confocal
Streptococcus mutans
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Plasma Gases
Ionized gases, consisting of free electrons and ionized atoms or molecules which collectively behave differently than gas, solid, or liquid. Plasma gases are used in biomedical fields in surface modification; biological decontamination; dentistry (e.g., PLASMA ARC DENTAL CURING LIGHTS); and in other treatments (e.g., ARGON PLASMA COAGULATION).
Microbial Interactions
Cetylpyridinium
Quorum Sensing
Peracetic Acid
Disinfectants
Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Gene Expression Regulation, Bacterial
Microbial Sensitivity Tests
Dental Plaque
Antifungal Agents
Chlorhexidine
Anti-Infective Agents, Local
Mouth
Staphylococcus aureus
Tobramycin
Equipment Contamination
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Bioelectric Energy Sources
Bioreactors
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Actinomyces
Farnesol
A colorless liquid extracted from oils of plants such as citronella, neroli, cyclamen, and tuberose. It is an intermediate step in the biological synthesis of cholesterol from mevalonic acid in vertebrates. It has a delicate odor and is used in perfumery. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Water Microbiology
Stainless Steel
Echinocandins
Cyclic hexapeptides of proline-ornithine-threonine-proline-threonine-serine. The cyclization with a single non-peptide bond can lead them to be incorrectly called DEPSIPEPTIDES, but the echinocandins lack ester links. Antifungal activity is via inhibition of 1,3-beta-glucan synthase production of BETA-GLUCANS.
Fusobacterium nucleatum
Drug Resistance, Fungal
Biomass
Silicones
Catheter-Related Infections
Silicone Elastomers
Polymers of silicone that are formed by crosslinking and treatment with amorphous silica to increase strength. They have properties similar to vulcanized natural rubber, in that they stretch under tension, retract rapidly, and fully recover to their original dimensions upon release. They are used in the encapsulation of surgical membranes and implants.
Catheters
Polyvinyl Chloride
Tetrazolium Salts
Quaternary salts derived from tetrazoles. They are used in tests to distinguish between reducing sugars and simple aldehydes, for detection of dehydrogenase in tissues, cells, and bacteria, for determination of corticosteroids, and in color photography. (From Mall's Dictionary of Chemistry, 5th ed, p455)
Erythrosine
Streptococcus gordonii
Alginates
RNA, Ribosomal, 16S
Fluconazole
Drug Resistance, Bacterial
Candida
A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)
Polystyrenes
Shewanella
Antibiosis
Resistance of artificial biofilms of Pseudomonas aeruginosa to imipenem and tobramycin. (1/4882)
Viable cells of Pseudomonas aeruginosa were entrapped in alginate gel layers and incubated in a minimal glucose (15 g/L)-yeast extract (2 g/L)-salt medium to form artificial biofilm-like structures. After cultivation for 2 days, the biomass distribution inside the polymer was highly heterogeneous. The cell number reached approximately 1011 cells/g gel in the outer regions of the gel structures whereas the inner areas were less colonized (c. 10(8) cells g/gel). Killing of immobilized organisms by imipenem and tobramycin were compared with free-cell experiments (inoculum c. 10(9) cells/mL). Sessile-like bacteria displayed a higher resistance to the two antibiotics used alone or in combination than did suspended cells. Exposure for 10 h to 20 x MIC imipenem and 15 x MIC tobramycin reduced the number of viable immobilized bacteria to 0.3% and 3%, respectively, of the initial cell population, whereas these antibiotic concentrations were much more efficient (bactericidal) against free-cell cultures (5 log kill in 6 h). A synergic effect of tobramycin and imipenem was detected on bacterial suspensions but not on biofilm-like structures. Effective diffusivity measurements showed that the diffusion of imipenem in the alginate layer was not hindered. A slight but significant enhancement of beta-lactamase induction in immobilized cells as compared with their suspended counterparts was insufficient to explain the high resistance of sessile-like bacteria. (+info)Vibrio cholerae O1 El Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation. (2/4882)
The rugose colony variant of Vibrio cholerae O1, biotype El Tor, is shown to produce an exopolysaccharide, EPSETr, that confers chlorine resistance and biofilm-forming capacity. EPSETr production requires a chromosomal locus, vps, that contains sequences homologous to carbohydrate biosynthesis genes of other bacterial species. Mutations within this locus yield chlorine-sensitive, smooth colony variants that are biofilm deficient. The biofilm-forming properties of EPSETr may enable the survival of V. cholerae O1 within environmental aquatic habitats between outbreaks of human disease. (+info)Surface-grafted, environmentally sensitive polymers for biofilm release. (3/4882)
Controlling bacterial biofouling is desirable for almost every human enterprise in which solid surfaces are introduced into nonsterile aqueous environments. One approach that is used to decrease contamination of manufactured devices by microorganisms is using materials that easily slough off accumulated material (i.e., fouling release surfaces). The compounds currently used for this purpose rely on low surface energy to inhibit strong attachment of organisms. In this study, we examined the possible use of environmentally responsive (or "smart") polymers as a new class of fouling release agents; a surface-grafted thermally responsive polymer, poly(N-isopropylacrylamide) (PNIPAAM), was used as a model compound. PNIPAAM is known to have a lower critical solubility temperature of approximately 32 degrees C (i.e., it is insoluble in water at temperatures above 32 degrees C and is soluble at temperatures below 32 degrees C). Under experimental conditions, >90% of cultured microorganisms (Staphylococcus epidermidis, Halomonas marina) and naturally occurring marine microorganisms that attached to grafted PNIPAAM surfaces during 2-, 18-, 36-, and 72-h incubations were removed when the hydration state of the polymer was changed from a wettability that was favorable for attachment to a wettability that was less favorable. Of particular significance is the observation that an organism known to attach in the greatest numbers to hydrophobic substrata (i.e., H. marina) was removed when transition of PNIPAAM to a more hydrated state occurred, whereas an organism that attaches in the greatest numbers to hydrophilic substrata (i.e., S. epidermidis) was removed when the opposite transition occurred. Neither solvated nor desolvated PNIPAAM exhibited intrinsic fouling release properties, indicating that the phase transition was the important factor in removal of organisms. Based on our observations of the behavior of this model system, we suggest that environmentally responsive polymers represent a new approach for controlling biofouling release. (+info)Ultrasonic enhancement of antibiotic action on Escherichia coli biofilms: an in vivo model. (4/4882)
Biofilm infections are a common complication of prosthetic devices in humans. Previous in vitro research has determined that low-frequency ultrasound combined with aminoglycoside antibiotics is an effective method of killing biofilms. We report the development of an in vivo model to determine if ultrasound enhances antibiotic action. Two 24-h-old Escherichia coli (ATCC 10798) biofilms grown on polyethylene disks were implanted subcutaneously on the backs of New Zealand White female rabbits, one on each side of the spine. Low-frequency (28.48-kHz) and low-power-density (100- and 300-mW/cm2) continuous ultrasound treatment was applied for 24 h with and without systemic administration of gentamicin. The disks were then removed, and the number of viable bacteria on each disk was determined. At the low ultrasonic power used in this study, exposure to ultrasound only (no gentamicin) caused no significant difference in bacterial viability. In the presence of antibiotic, there was a significant reduction due to 300-mW/cm2 ultrasound (P = 0.0485) but no significant reduction due to 100-mW/cm2 ultrasound. Tissue damage to the skin was noted at the 300-mW/cm2 treatment level. Further development of this technique has promise in treatment of clinical implant infections. (+info)Study of the response of a biofilm bacterial community to UV radiation. (5/4882)
We have developed a bioluminescent whole-cell biosensor that can be incorporated into biofilm ecosystems. RM4440 is a Pseudomonas aeruginosa FRD1 derivative that carries a plasmid-based recA-luxCDABE fusion. We immobilized RM4440 in an alginate matrix to simulate a biofilm, and we studied its response to UV radiation damage. The biofilm showed a protective property by physical shielding against UV C, UV B, and UV A. Absorption of UV light by the alginate matrix translated into a higher survival rate than observed with planktonic cells at similar input fluences. UV A was shown to be effectively blocked by the biofilm matrix and to have no detectable effects on cells contained in the biofilm. However, in the presence of photosensitizers (i.e., psoralen), UV A was effective in inducing light production and cell death. RM4440 has proved to be a useful tool to study microbial communities in a noninvasive manner. (+info)Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model. (6/4882)
The production of biofilm is thought to be crucial in the pathogenesis of prosthetic-device infections caused by Staphylococcus epidermidis. An experimental animal model was used to assess the importance of biofilm production, which is mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of a biomaterial-based infection. Mice were inoculated along the length of a subcutaneously implanted intravenous catheter with either wild-type S. epidermidis 1457 or its isogenic PIA/HA-negative mutant. The wild-type strain was significantly more likely to cause a subcutaneous abscess than the mutant strain (P < 0.01) and was significantly less likely to be eradicated from the inoculation site by host defense (P < 0.05). In addition, the wild-type strain was found to adhere to the implanted catheters more abundantly than the PIA/HA-negative mutant (P < 0.05). The reliability of the adherence assay was assessed by scanning electron microscopy. To exclude contamination or spontaneous infection, bacterial strains recovered from the experimental animals were compared to inoculation strains by analysis of restriction fragment length polymorphism patterns by pulsed-field gel electrophoresis. In vitro binding of the wild-type strain and its isogenic mutant to a fibronectin-coated surface was similar. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental foreign body infection. (+info)Characterization of Staphylococcus epidermidis polysaccharide intercellular adhesin/hemagglutinin in the pathogenesis of intravascular catheter-associated infection in a rat model. (7/4882)
Biofilm production is thought to be a crucial factor in the ability of Staphylococcus epidermidis to produce a biomaterial-based infection. A rat central venous catheter (CVC)-associated infection model was used to assess the importance of biofilm production, mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of intravascular catheter-associated infection. PIA/HA-positive S. epidermidis 1457 was significantly more likely to cause a CVC-associated infection (71 versus 14%, P < 0.03) resulting in bacteremia and metastatic disease than its isogenic PIA/HA-negative mutant. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental CVC-associated infection. (+info)Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis. (8/4882)
To determine whether a relationship exists between biofilm formation and hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and hemagglutination. Strains that produced biofilm were significantly (P<.001) more likely to mediate hemagglutination (16 biofilm-positive/hemagglutination-positive strains and 19 biofilm-negative/hemagglutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated hemagglutination (>/=1/128). It was concluded that production of PIA and hemagglutination are strongly associated and that PIA, at least in part, mediates hemagglutination in S. epidermidis. (+info)
The Roles of Glycerol in Candida Albicans Biofilm Formation and Invasi by Jigarkumar V. Desai
Biofilm formation on nanostructured hydroxyapatite-coated titanium. | NIOM
The role of the globin-coupled sensor YddV in a mature E. coli biofilm population - Institutional Repository University of...
Pseudomonas aeruginosa Promotes Escherichia coli Biofilm Formation in Nutrient-Limited Medium - Semantic Scholar
During the Early Stages of Staphylococcus aureus Biofilm Formation, Induced Neutrophil Extracellular Traps Are Degraded by...
IDEALS @ Illinois: Role of disinfectants and pipe materials on bacterial adhesion onto biofilms
Synergistic Effects in Mixed Escherichia coli Biofilms: Conjugative Plasmid Transfer Drives Biofilm Expansion | Journal of...
Abrasive treatment of microtiter plates improves the reproducibility of bacterial biofilm assays - RSC Advances (RSC Publishing)
Paper: Oral Epithelial Cell Responses to Multispecies Microbial Biofilms (AADR Annual Meeting (March 21-24, 2012))
Influence of Hydrodynamics and Cell Signaling on the Structure and Behavior of Pseudomonas aeruginosa Biofilms | Applied and...
Targeting Macrophage Activation for the Prevention and Treatment of Staphylococcus aureus Biofilm Infections | The Journal of...
Interactions of 1 m latex particles with pseudomonas aeruginosa biofilms
DNA-microarrays identification of Streptococcus mutans genes associated with biofilm thickness | BMC Microbiology | Full Text
Differentiation and Distribution of Colistin- and Sodium Dodecyl Sulfate-Tolerant Cells in Pseudomonas aeruginosa Biofilms |...
Signaling factor interactions with polysaccharide aggregates of bacterial biofilms<...
Co-operation and Group structure in Bacterial Biofilms - ePrints Soton
Contribution of biofilm matrix components to physical properties of Bacillus subtilis biofilms at all phases of biofilm...
Efficacy of citronella and cinnamon essential oils on Candida albicans biofilms -ORCA
Pesquisa | Portal Regional da BVS
Persister cell-mediated antimicrobial tolerance in pseudomonas aeruginosa biofilm populations - ePrints Soton
The composition and metabolic phenotype of Neisseria gonorrhoeae biofilms - UQ eSpace
Hydrolytic enzyme production is associated with Candida albicans biofilm formation from patients with type 1 diabetes -...
Session 2 Abstracts
Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix
The Application of Impedance Microsensors for Real-Time Analysis of Pseudomonas aeruginosa Biofilm Formation - PJM ONLINE
Fighting biofilms with lantibiotics and other groups of bacteriocins
Alexandru Mihai Grumezescu » Prevention of Microbial Biofilms - the Contribution of Micro and Nanostructured Materials.
QUANTIFYING THE EFFECT OF HYDRODYNAMIC SHEAR ON POLYMORPHONUCLEAR LEUKOCYTE ADHESION TO STAPHYLOCOCCUS AUREUS BIOFILMS :: UMBC...
It is all about location: how to pinpoint microorganisms and their functions in multispecies biofilms - Institut National de...
Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development | BMC Microbiology | Full Text
Biofilm formation inhibition and dispersal of multi-species communities containing ammonia-oxidising bacteria | npj Biofilms...
Biofilm Eradication and Prevention: A Pharmaceutical Approach to Medical Device Infections | Pharmaceutical & Medicinal...
Incorporation of chitosan in acrylic bone cement: effect on antibiotic release and bacterial biofilm formation.</em>...
The small molecule DAM inhibitor, pyrimidinedione, disrupts Streptococcus pneumoniae biofilm growth in vitro<...
Staphylococcus aureus CcpA Affects Biofilm Formation | Infection and Immunity
Biology Spring Seminar Series: Molecular Insights into Bacterial Biofilm Formation in the Cystic Fibrosis Lung | Indiana State...
Dynamics of biofilm processes
Genome rearrangements induce biofilm formation in Escherichia coli C - an old model organism with a new application in biofilm...
Biofilms - a P.A.K. Approach
Conservation of acquired morphology and community structure in aged biofilms after facing environmental stress - Institut...
Biofilms may help cause ear infections » UF Health Podcasts - University of Florida Health
Lyme Warrior Shares Dr. Eve Sapis Study on Biofilms
Keratitis-Associated Fungi Form Biofilms with Reduced Antifungal Drug Susceptibility | IOVS | ARVO Journals
Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili. - PubMed -...
Towards individualized diagnostics of biofilm-associated infections: a case study. - Helmholtz Zentrum für Infektionsforschung...
Pharmacodynamic and Immunomodulatory Effects of Micafungin on Host Responses against Biofilms of Candida parapsilosis in...
Genetic Control Of Candida Albicans Biofilm Development - Yeast Infection and Candida Albicans
Accumulation-associated protein enhances Staphylococcus epidermidis biofilm formation under dynamic conditions and is required...
Inhibitory effect of zinc oxide nanoparticles on pseudomonas aeruginosa biofilm formation - Mashhad University of Medical...
Universidade do Minho: The effect of silver nanoparticles and nystatin on mixed biofilms of Candida glabrata and Candida...
In vitro Streptococcus mutans biofilm formation on surfaces of chlorhexidine-containing dentin bonding systems | Archivio della...
Frontiers | Corrigendum: Anti-quorum Sensing and Anti-biofilm Activity of Delftia tsuruhatensis Extract by Attenuating the...
Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains | BMC...
Temperature, pH and trimethoprim-sulfamethoxazole are potent inhibitors of biofilm formation by Stenotrophomonas maltophilia...
Microbiology Society Journals | Iron-binding compounds impair Pseudomonas aeruginosa biofilm formation, especially under...
Acervo Digital: In vitro Activity of Daptomycin, Linezolid and Rifampicin on Staphylococcus epidermidis Biofilms
The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner |...
Impact of the broad-spectrum antimicrobial peptide, lacticin 3147, on Streptococcus mutans growing in a biofilm and in human...
Frontiers | A Negative Regulator of Cellulose Biosynthesis, bcsR, Affects Biofilm Formation, and Adhesion/Invasion Ability of...
In vitro anti-biofilm activity of Quercus brantii subsp. persica on human pathogenic bacteria
Model system for growing and quantifying Streptococcus pneumoniae biofilms in situ and in real time | Laser Dynamics Lab
Pathogens | Free Full-Text | Microbial Biofilms in Urinary Tract Infections and Prostatitis: Etiology, Pathogenicity, and...
KEGG PATHWAY: Biofilm formation - Vibrio cholerae + T30050
Inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming Lactobacillus isolate: a potential host for the...
Influence of microbial interactions and EPS/polysaccharide composition on nutrient removal activity in biofilms formed by...
View source for Streptococcus mutans biofilm - microbewiki
Membrane Aerated Biofilm Reactor (MABR) Market Study Based on Shares, Current Opportunities with Future Growth Scenario by 2027...
Biofilms, infection, and antimicrobial therapy</em>...
Controlling biofilm development on ultrafiltration and reverse osmosis membranes used in dairy plants : a thesis presented in...
An update on Pseudomonas aeruginosa biofilm formation, tolerance, and dispersal
The Inhibitory Effects of a Novel Gel on Staphylococcus aureus Biofilm by Lindsey Vance
Streptococcus pneumoniae Modulates Staphylococcus aureus Biofilm Dispersion and the Transition from Colonization to Invasive...
Salmonella in biofilms proves highly resistant to disinfectants
Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro...
Targeting quorum sensing in Pseudomonas aeruginosa biofilms - Danish National Research Database-Den Danske Forskningsdatabase
Brominated furanones inhibit biofilm formation by Salmonella enterica serovar Typhimurium
Microcontainer Delivery of Antibiotic Improves Treatment of Pseudomonas aeruginosa Biofilms<...
Dose-Dependent Effects of Common Antibiotics Used to Treat Staphylococcus aureus on Biofilm Formation
Sfp-type PPTase inactivation promotes bacterial biofilm formation and ability to enhance wheat drought tolerance
KEGG PATHWAY: Biofilm formation - Vibrio cholerae
KEGG PATHWAY: Biofilm formation - Vibrio cholerae
Competition and Caries on Enamel of a Dual-Species Biofilm Model with Streptococcus mutans and Streptococcus sanguinis |...
Cyclic diguanylate (c-di-GMP) regulates Vibrio cholerae biofilm formation
σX Is Involved in Controlling Bacillus subtilis Biofilm Architecture through the AbrB Homologue Abh - Research Database, The...
Post-translational control of Bacillus subtilis biofilm formation mediated by tyrosine phosphorylation - Research Database, The...
Influence of serum and polystyrene plate type on stability of Candida albicans biofilms. - NextBio article
Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from...
Effect of Polyphenol-Rich Cranberry Extracts on Cariogenic Biofilm Properties and Microbial Composition of Polymicrobial...
Get PDF - Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to...
Iron and Acinetobacter baumannii Biofilm Formation | Università degli studi Roma Tre
Streptococcus mutans biofilm - microbewiki
Staphylococcus haemolyticus
Biofilm formation[edit]. The ability to adhere to medical devices and subsequently form biofilms is a major virulence factor ... Biofilm formation also appears to be influenced by the presence of glucose and NaCl. Biofilm formation is enhanced when ... haemolyticus biofilms. Biofilms formed in the presence of subinhibitory concentrations of dicloxacillin contain less biomass ... Biofilm formation is influenced by a variety of factors including carbohydrates, proteins, and extracellular DNA. Detachment ...
Corrosion
Biofilm coatings[edit]. A new form of protection has been developed by applying certain species of bacterial films to the ... Alternatively, antimicrobial-producing biofilms can be used to inhibit mild steel corrosion from sulfate-reducing bacteria.[6] ... "Inhibiting mild steel corrosion from sulfate-reducing bacteria using antimicrobial-producing biofilms in Three-Mile-Island ...
Roberto Kolter
Genetic approaches to study of biofilms". Biofilms. Methods in Enzymology. Biofilms. 310. Academic Press. pp. 91-109. doi: ... a TED-ED animation on biofilms Biofilm Up Close, FASEB Bioart Award-winning image in The Scientist in 2016 Turning Point: ... but the genetics of biofilm formation was unexplored and most microbiologists did not view biofilm formation as a physiological ... living within communities called biofilms). The lab popularized the concept of bacterial biofilm formation as developmental or ...
Streptococcus mutans
Biofilm is an aggregate of microorganisms in which cells adhere to each other or a surface. Bacteria in the biofilm community ... This system functions optimally when the S. mutans cells are in crowded biofilms. S. mutans cells growing in a biofilm are ... mutans in dental biofilms can be reduced or eliminated, the acidification potential of dental biofilms and later cavity ... This decreases the biofilm pathogenesis, and therefore its caries promoting potential. This offers the potential for an anti- ...
Cellulose
Some species of bacteria secrete it to form biofilms. Cellulose is the most abundant organic polymer on Earth. The cellulose ... Romeo, Tony (2008). Bacterial biofilms. Berlin: Springer. pp. 258-263. ISBN 978-3-540-75418-3. Klemm, Dieter; Heublein, ...
Shewanella oneidensis
Pellicle is a variety of biofilm that is formed between the air and the liquid in which bacteria grow. In a biofilm, bacterial ... The biofilm needs bacterial cells to move in a certain manner, while flagella is the organelle which has locomotive function. ... Lemon, KP; Earl, AM; Vlamakis, HC; Aguilar, C; Kolter, R. "Biofilm development with an emphasis on Bacillus subtilis". ... Bacterial Biofilms. 2008: 1-16. PMID 18453269. Pratt, Leslie A.; Kolter, Roberto (1998). "Genetic analysis ofEscherichia ...
Cis-2-Decenoic acid
It may have potential in fighting biofilm implied in infectious diseases that are present in more than 60% of Hospital-acquired ... Marques, CN; Davies, DG; Sauer, K (2015). "Control of Biofilms with the Fatty Acid Signaling Molecule cis-2-Decenoic Acid". ... Davies, DG; Marques, CN (2009). "A fatty acid messenger is responsible for inducing dispersion in microbial biofilms". Journal ... Bryers, JD (2008). "Medical biofilms". Biotechnol. Bioeng. 100 (1): 1-18. doi:10.1002/bit.21838. PMC 2706312. PMID 18366134. v ...
Protein adsorption in the food industry
A biofilm is a community of microorganisms adsorbed to a surface. Microorganisms in biofilms are enclosed in a polymeric matrix ... Biofilms on food processing surfaces can be a biological hazard to food safety. Increased chemical resistance in biofilms can ... Biofilms form on solid substrates such as stainless steel. A biofilm's enclosing polymeric matrix offers protection to its ... Tarver, Toni (2009). "Biofilms: A Threat to Food Safety". Food Technology. 63 (2): 46-52. Visser, J; Jeurnink, Th. J. M (1997 ...
Carol Kumamoto
Kumamoto, Carol A. (2006). "Candida albicans Biofilms Produce Antifungal-Tolerant Persister Cells". Antimicrobial Agents and ... "Candida biofilms". Current Opinion in Microbiology. 5 (6): 608-611. doi:10.1016/S1369-5274(02)00371-5. PMID 12457706. ...
Candida albicans
Such C. albicans biofilms may form on the surface of implantable medical devices or organs. In these biofilms it is often found ... "Candida albicans biofilm development is governed by cooperative attachment and adhesion maintenance proteins". NPJ Biofilms and ... The biofilm of C. albicans is formed in four steps. First, there is the initial adherence step, where the yeast-form cells ... Zap1 controls the equilibrium of yeast and hyphal cells, the zinc transporters and zinc regulated genes in biofilms of C. ...
Prokaryote
When such communities are encased in a stabilizing polymer matrix ("slime"), they may be called "biofilms". Cells in biofilms ... Costerton JW (2007). "Direct Observations". The Biofilm Primer. Springer Series on Biofilms. 1. pp. 3-4. doi:10.1007/978-3-540- ... Bacterial biofilms may be 100 times more resistant to antibiotics than free-living unicells and may be nearly impossible to ... Biofilms may be highly heterogeneous and structurally complex and may attach to solid surfaces, or exist at liquid-air ...
Bacteria
These biofilms and mats can range from a few micrometres in thickness to up to half a metre in depth, and may contain multiple ... Biofilms are also important in medicine, as these structures are often present during chronic bacterial infections or in ... Bacteria living in biofilms display a complex arrangement of cells and extracellular components, forming secondary structures, ... In natural environments, such as soil or the surfaces of plants, the majority of bacteria are bound to surfaces in biofilms. ...
Staphylococcus aureus
The biofilm formation is the main cause of Staphylococcus implant infections. Biofilms are groups of microorganisms, such as ... A class of enzymes have been found to have biofilm matrix-degrading ability, thus may be used as biofilm dispersal agents in ... A higher mortality is linked with multispecies biofilms. S. aureus biofilm is the predominant cause of orthopedic implant- ... aureus biofilms contain PIA. S. aureus biofilms are important in disease pathogenesis, as they can contribute to antibiotic ...
Phagoburn
"Bacteriophages and Biofilms". Antibiotics. 3 (3): 270-284. doi:10.3390/antibiotics3030270. PMC 4790368. Patrick Jault; Thomas ...
Staphylococcus epidermidis
It allows other bacteria to bind to the already existing biofilm, creating a multilayer biofilm. Such biofilms decrease the ... The ability to form biofilms on plastic devices is a major virulence factor for S. epidermidis. One probable cause is surface ... Biofilms Microbiology Staphylococcus Schleifer, K. H.; Kloos, W. E. (1 January 1975). "Isolation and Characterization of ... As mentioned above, S. epidermidis causes biofilms to grow on plastic devices placed within the body. This occurs most commonly ...
Peptidoglycan
The peptidoglycan monomers are synthesized in the cytosol and are then attached to a membrane carrier bactoprenol. Bactoprenol transports peptidoglycan monomers across the cell membrane where they are inserted into the existing peptidoglycan.[6] In the first step of peptidoglycan synthesis, glutamine, which is an amino acid, donates an amino group to a sugar, fructose 6-phosphate. This turns fructose 6-phosphate into glucosamine-6-phosphate. In step two, an acetyl group is transferred from acetyl CoA to the amino group on the glucosamine-6-phosphate creating N-acetyl-glucosamine-6-phosphate.[7] In step three of the synthesis process, the N-acetyl-glucosamine-6-phosphate is isomerized, which will change N-acetyl-glucosamine-6-phosphate to N-acetyl-glucosamine-1-phosphate.[7] In step 4, the N-acetyl-glucosamine-1-phosphate, which is now a monophosphate, attacks UTP. Uridine triphosphate, which is a pyrimidine nucleotide, has the ability to act as an energy source. In this particular reaction, ...
Bacterial phyla
The bacterial phyla are the major lineages, known as phyla or divisions, of the domain Bacteria. In the scientific classification established by Carl von Linné,[2] each bacterial strain has to be assigned to a species (binary nomenclature), which is a lower level of a hierarchy of ranks. Currently, the most accepted mega-classification system is under the three-domain system, which is based on molecular phylogeny. In that system, bacteria are members of the domain Bacteria[3] and "phylum" is the rank below domain, since the rank "kingdom" is disused at present in bacterial taxonomy.[4][Note 1] When bacterial nomenclature was controlled under the Botanical Code, the term division was used, but now that bacterial nomenclature (with the exception of cyanobacteria) is controlled under the Bacteriological Code, the term phylum is preferred. In this classification scheme, Bacteria is (unofficially)[Note 2] subdivided into 30 phyla with representatives cultured in a lab.[5][6][7] Many major clades of ...
Catabolite repression
... was extensively studied in Escherichia coli. E. coli grows faster on glucose than on any other carbon source. For example, if E. coli is placed on an agar plate containing only glucose and lactose, the bacteria will use glucose first and lactose second. When glucose is available in the environment, the synthesis of β-galactosidase is under repression due to the effect of catabolite repression caused by glucose. The catabolite repression in this case is achieved through the utilization of phosphotransferase system. An important enzyme from the phosphotransferase system called Enzyme II A (EIIA) plays a central role in this mechanism. There are different catabolite-specific EIIA in a single cell, even though different bacterial groups have specificities to different sets of catabolites. In enteric bacteria one of the EIIA enzymes in their set is specific for glucose transport only. When glucose levels are high inside the bacteria, EIIA mostly exists in its unphosphorylated ...
Gram-negative bacteria
Bacteria are traditionally divided into the two groups: gram-positive and gram-negative, based on their gram-staining response. Gram-positive bacteria are also referred to as monoderms having one membrane, and gram-negative bacteria are also referred to as diderms, having two membranes. These groups are often thought of as lineages, with gram-negative bacteria more closely related to one another than to gram-positive bacteria. While this is often true, the classification system breaks down in some cases. A given bacteria's staining result, bacterial membrane organization, and lineage groupings do not always match up.[6][7][8][9] Thus, gram-staining cannot be reliably used to assess familial relationships of bacteria. However, staining often gives reliable information about the composition of the cell membrane, distinguishing between the presence or absence of an outer lipid membrane.[6][10] Of these two structurally distinct groups of prokaryotic organisms, monoderm prokaryotes are indicated to ...
GFAJ-1
NASA's announcement of a news conference "that will impact the search for evidence of extraterrestrial life" was criticized as sensationalistic and misleading; an editorial in New Scientist commented "although the discovery of alien life, if it ever happens, would be one of the biggest stories imaginable, this was light-years from that".[31][32] In addition, many experts who have evaluated the paper have concluded that the reported studies do not provide enough evidence to support the claims made by the authors.[33] In an online article on Slate, science writer Carl Zimmer discussed the skepticism of several scientists: "I reached out to a dozen experts ... Almost unanimously, they think the NASA scientists have failed to make their case".[34][35] Chemist Steven A. Benner has expressed doubts that arsenate has replaced phosphate in the DNA of this organism. He suggested that the trace contaminants in the growth medium used by Wolfe-Simon in her laboratory cultures are sufficient to supply the ...
Arabinogalactan
The reducing end of microbial arabinogalactan consists of the terminal sequence →5)-D-Galf-(1→4)-L-Rhap-(1→3)-D-GlcNAc[citation needed]. A muramyl-6-P is also found within the peptidoglycan functional group. The mycolylarabinogalactan of mycobacteria is attached to the peptidoglycan by the actinomycete-specific diglycosylphosphoryl bridge, L-Rhap-(1→3)-D-GlcNAc-(1→P).[3]. Arabinogalactan contains a galactan chain, with alternating 5-linked β-D-galactofuranosyl (Galf) and 6-linked β-D-Galf residues. The arabinan chains are attached to C-5 of some of the 6-linked Galf residues. There are three major structural domains for arabinan. The first is a domain consisting of linear 5-linked α-D-Araf residues. The second is a domain with branched 3,5 linked α-D-Araf residues substituted with 5-linked α-D-Araf units at both branched positions, and the third is A terminal non-reducing domain for end arabinan consisting of a 3,5-linked α-D-Araf residue substituted at both branched positions ...
Sponge
NPJ Biofilms and Microbiomes. 4 (1): 14. doi:10.1038/s41522-018-0057-2. PMC 6031612. PMID 30002868. Bergquist PR (1998). " ...
Ctenophora
NPJ Biofilms and Microbiomes. 4 (1): 14. doi:10.1038/s41522-018-0057-2. PMC 6031612. PMID 30002868. Whelan, Nathan V.; Kocot, ...
Agent-based model in biology
Biofilm structures that are formed in simulation can be viewed as a movie using POV-Ray files that are generated as the ... This study explored the hypothesis that poor plasmid spread in biofilms is caused by a dependence of conjugation on the growth ... iDynoMiCS can be used to seek to understand how individual microbial dynamics lead to emergent population- or biofilm-level ... In the article titled "iDynoMiCS: next-generation individual-based modelling of biofilms", an agent-based model is presented ...
Biomineralization
"Spatio-temporal assembly of functional mineral scaffolds within microbial biofilms". NPJ Biofilms and Microbiomes. 2: 15031. ... The term biofilm refers to complex heterogeneous structures comprising different populations of microorganisms that attach and ... It uses the polymers produced by single cells during biofilm formation as a physical cue to coordinate ECM production by the ... A common principle of all biofilms is the production of extracellular matrix (ECM) composed of different organic substances, ...
Sphingomonadaceae
Some species from Sphingomonadaceae family are dominant components of biofilms. There are eighteen genera in Sphingomonadaceae ... 2019-01-25). "Sphingomonadaceae from fouled membranes". NPJ Biofilms and Microbiomes. 5 (1): 6. doi:10.1038/s41522-018-0074-1. ... May 2016). Besser TE (ed.). "Biofilms on Hospital Shower Hoses: Characterization and Implications for Nosocomial Infections". ... "Dynamics of the physiochemical and community structures of biofilms under the influence of algal organic matter and humic ...
Antimicrobial peptides
"The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilms". NPJ Biofilms and Microbiomes. 6 ... Cecropin A can destroy planktonic and sessile biofilm-forming uropathogenic E. coli (UPEC) cells, either alone or when combined ... The multi-target mechanism of action involves outer membrane permeabilization followed by biofilm disruption triggered by the ...
Liane G. Benning
NPJ Biofilms and Microbiomes. 3 (1): 10. doi:10.1038/s41522-017-0019-0. ISSN 2055-5008. PMC 5460203. PMID 28649411. "Microbial ...
Komodo dragon
"Komodo dragon-inspired synthetic peptide DRGN-1 promotes wound-healing of a mixed-biofilm infected wound". NPJ Biofilms and ... It has the added observed benefit of significantly promoting wound healing in both uninfected and mixed biofilm infected wounds ...
Metaproteomics
... npj Biofilms and Microbiomes. 6 (1): 14. doi:10.1038/s41522-020-0123-4. ISSN 2055-5008. PMC 7093434. PMID 32210237. Xiong, ...
biofilms
more on biofilm. so biofilms forming on food particles means that yogurt is a biofilm,. just devoid of large scale structural ... strep is a biofilm. ankylosing spondylitis is a biofilm condition. allergies can be caused by biofilm toxins pubmed. staph ... gallstones are biofilm. UTIS are biofilm. saccharomyces and clostridia. saccharomyces a poor biofilm former compared to ... franz xaver messerschmidt - biofilm sculpture usman on biofilm - the BCD still rules. the biofilm bandwagon slowly gathers ...
Biofilms - [email protected]
Biofilms Biofilms are bacterial colonies that are attached to a surface. They grow from suspended, or planktonic, cells ... We are investigating the dynamics of biofilm growth using experimental, simulation, and mathematical modeling methods. This ... attaching to the surface and by cell division and movement of existing attached cells. Biofilms are found naturally in many ...
Biofilms. - PubMed - NCBI
Biofilms.. Stickler D1.. Author information. 1. Cardiff School of Biosciences, Cardiff University, Cardiff, CF1 3TL, Wales, UK ... The past year has seen important advances in our understanding of how cells initiate biofilm formation. We have also begun to ... Outside of the laboratory, most microbes grow as organised biofilm communities on surfaces. ... appreciate how cells can co-ordinate their activities and build the complex structures of mature biofilms that afford ...
biofilms Archives | Popular Science
Egalitarianism in Biofilms | SpringerLink
Microbial biofilms are multicellular communities of sessile microorganisms encased by the hydrated polymeric matrix. They have ... By measuring the biofilm growing rates under different evenness levels of communities, an evenly distributed community favors ... Microbial biofilms are multicellular communities of sessile microorganisms encased by the hydrated polymeric matrix. They have ... Humphries J, Xiong L, Liu J, Prindle A, Yuan F, Arjes HA et al (2017) Species-independent attraction to biofilms through ...
Springer Series on Biofilms
Once anchored to a surface, biofilm microorganisms carry out a ... ... Springer Series on Biofilms presents carefully refereed volumes on selected topics on this field of research. All volumes ... or the roles of biofilms in diseases. While based in microbiology, biofilms are of intense interest to many other scientists, ... Springer Series on Biofilms presents carefully refereed volumes on selected topics on this field of research. All volumes ...
Protein Disrupts Infectious Biofilms | Caltech
"In part, the reason these infections are hard to treat is because P. aeruginosa enters a biofilm mode of growth in these ... Now, a group of researchers at Caltech and the University of Oxford have made progress in the fight against biofilms. Led by ... Our research suggests a new approach to inhibiting P. aeruginosa biofilms.". The group targeted pyocyanin, a small molecule ... Many infectious pathogens are difficult to treat because they develop into biofilms, layers of metabolically active but slowly ...
biofilm | PNAS
The sociobiology of biofilms. - PubMed - NCBI
First, the appearance of organization in biofilms can emerge without active coordination. That is, biofilm properties such as ... The sociobiology of biofilms.. Nadell CD1, Xavier JB, Foster KR.. Author information. 1. Department of Ecology and Evolutionary ... and spontaneous mutation can generate conflict even within biofilms initiated by genetically identical cells. Biofilms will ... Biofilms are densely packed communities of microbial cells that grow on surfaces and surround themselves with secreted polymers ...
Chlorhexidine Effective Against Candida auris Biofilms
The study showed that C auris can form a biofilm that resists many antifungal agents. A biofilm also enables the fungus to use ... To better understand the fungus biofilm capabilities, the researchers compared the biofilm mass of four strains of C auris ... The C auris strains developed 1.5 to 3 times less biofilm mass than C albicans, but 3 to 6 times greater mass than C glabrata, ... The C auris biofilm is not as strong as those of some other clinically significant fungi, but has nonetheless contributed to C ...
Biofilms: How microbial communities outfox antibiotics
So, when faced with biofilm infections, what can doctors do?. The battle against biofilms. Biofilms are a serious threat to ... What are biofilms?. "Biofilms are one of the most widely distributed and successful modes of life on Earth," says Prof. Hans- ... Biofilms can be made up of populations of the same bacteria or of communities, which, in turn, are made up of many different ... Biofilms are tricky beasts because they have a tendency to become resistant to all manner of efforts employed to eradicate them ...
Characterizing Bacteriophage-Derived Enzymes That Degrade Staphylococcus Biofilms
Biofilms are surface-attached communities of bacterial or fungal cells that are enmeshed in an extensive ... the minimum biofilm eradicating concentration that removes 50% of the biofilm) for each treatment. In the ongoing effort to ... Biofilms are surface-attached communities of bacterial or fungal cells that are enmeshed in an extensive extracellular matrix ... With biofilms estimated to be responsible for ,60% of microbial infections, and 80% of chronic infections in humans, finding ...
Opportunistic pathogens enriched in showerhead biofilms | PNAS
The biofilm assemblages were comprised of ubiquitous water and soil microbial groups, some known for biofilm formation. ... Showerhead biofilms and water are potential sources of aerosolized microorganisms. However, different microbes and biofilms ... Fluorescence and SEM images of showerhead biofilm. (A-C) Epifluorescence microscopy of biofilm samples stained with DAPI; scale ... Opportunistic pathogens enriched in showerhead biofilms. Leah M. Feazel, Laura K. Baumgartner, Kristen L. Peterson, Daniel N. ...
How flow shapes bacterial biofilms | EurekAlert! Science News
The findings can give us clues about the physical rules guiding biofilm architecture, but also about the social dynamics that ... EPFL biophysicists have taken a systematic look into how bacterial biofilms are affected by fluid flow. ... How flow shapes bacterial biofilms EPFL biophysicists have taken a systematic look into how bacterial biofilms are affected by ... Bacteria form biofilms by attaching to each other on a wide variety of surfaces: the bottom of oceans, lakes or rivers, medical ...
Bacterial biofilms under the microscope | UCLouvain
Many infections contracted in hospital are linked to the formation of biofilms. How do these sticky layers of bacteria form on ... What is biofilm?. Indeed, bacteria such as staphylococcus aureus can attach themselves to medical device surfaces (. catheter. ... This can entail grave complications for the patient, which is why its so important to study bacterial biofilms and find a way ... Many infections contracted in hospital are linked to the formation of biofilms. How do these sticky layers of bacteria form on ...
Microbial Biofilms and Breast Tissue Expanders
Microphytobenthic Biofilms: Composition and Interactions | SpringerLink
Microphytobenthic biofilms in mudflats are characterised by a wide variety of microorganisms and the production of large ... Flemming H-C, Wingender J (2010) The biofilm matrix. Nat Rev Microbiol 8:623-633PubMedPubMedCentralCrossRefGoogle Scholar ... Sutherland I (2001) Biofilm exopolysaccharides: a strong and sticky framework. Microbiology 147:3-9PubMedCrossRefPubMedCentral ... Branda SS, Vik S, Friedman L, Kolter R (2005) Biofilms: the matrix revisited. Trends Microbiol 13:20-26PubMedCrossRef ...
Medical implants coated with 'slippery surfaces' repel infectious biofilms
Scientists develop a slippery surface for medical implants that prevents formation of infectious bacterial biofilm while ... SLIPS resisted biofilms and preserved immune response. In the new study, the team started with lab tests to find the teflon ... In medicine, biofilms are a hazard because they can form on living surfaces, such as heart and lung tissue, and on medical ... The study of biofilms has increased rapidly in recent years as we have become more aware of their pervasiveness and impact. The ...
Survival of the Slimiest: The Persistent Nature of Biofilms
Biofilms are hardier than we imagined, playing an underappreciated role in nosocomial transmission. ... The Role of Biofilm in Nosocomial Transmission. Biofilms are sessile microbial communities growing on surfaces, frequently ... How Hidden Biofilms Escape Disinfection. Alfa and colleagues[2] looked at the impact of improper positioning of the elevator ... Biofilm formation and bacterial viability during the survival analysis were assessed. The culturability of the A pittii strains ...
Life Cycle of Biofilms: Before Attachment | HowStuffWorks
The life cycle of biofilms is explained in this section. Learn about the life cycle of biofilms. ... Life Cycle of Biofilms: Before Attachment. Before we delve into the life cycle of a biofilm, its important to get a sense of ... Biofilms in Space. Trying to study biofilms on Earth is one thing, but what about in space? Researchers at the Rensselaer ... Different species of bacteria, protozoans, algae, yeasts and fungi can form biofilms. With most biofilms ranging from a few ...
Host Defence against Bacterial Biofilms: "Mission Impossible"?
biofilms," Journal of Bacteriology, vol. 182, no. 22, pp. 6482-6489, 2000. View at Publisher · View at Google Scholar · View at ... "Gene transfer occurs with enhanced efficiency in biofilms and induces enhanced stabilisation of the biofilm structure," Current ... M. Otto, "Staphylococcal biofilms," Current Topics in Microbiology and Immunology, vol. 322, pp. 207-228, 2008. View at Google ... A. S. Prince, "Biofilms, antimicrobial resistance, and airway infection," New England Journal of Medicine, vol. 347, no. 14, pp ...
The architect of the biofilm | Nature Reviews Microbiology
... dynamics of a matrix protein ofVibrio choleraebiofilms and examined how this protein modulates the architecture of the biofilm. ... Vibrio cholerae, the causative agent of cholera, can switch between single cell and biofilm lifestyles. Biofilm formation has ... To test whether the structural binary switch regulated biofilm formation, the authors analysed biofilm development and ... Living in the matrix: assembly and control of Vibrio cholerae biofilms. Nat. Rev. Microbiol. 13, 255-268 (2015) ...
Predicting and Managing Biofilms - Eawag
As biofilms influence both potable water quality as well as stability, it is important to generate a deeper insight on factors ... Predicting and Managing Biofilms. Materials in contact with potable water variably influence the existing microbiome due to ... Therefore, biofilms on the inner surface of several toys, used under either real or controlled conditions, have been ... The goal of this SNF-funded project is to properly characterize biofilms on materials in contact with potable water and to ...
Control of Pathogens in Biofilms
... March 2014. Various bacteria, including foodborne pathogens, can form biofilms on stainless ... Results of this study indicate that treatment of L. monocytogenes, S. Typhimurium, and STEC biofilms with lactic acid and ... Biofilms on treated and untreated coupons were subsequently enriched by immersing coupons in BHI or TSB, and in aqueous ... Populations of 8.6 to 9.2 log CFU of pathogen/coupon were recovered from biofilms after incubating in BHI and TSB for 72 h. ...
Biofilms] Re: Surface energy - meaning?
... Dev via biofilms%40net.bio.net (by microbesinaction At gmail.com). Fri Dec 1 01:33:15 ... It is also well known that hydrophobicity has a role in cell attachment to the surface and biofilm formation. As the biofilm ... www.bio.net/biomail/listinfo/biofilms , , brooksjd.vcf , 1KDownload * Messages sorted by: [ date ] [ thread ] [ subject ] [ ... Regards , John , , Alfred Ogola Okello wrote: , , Dear Sir, , , , Ref:http://www.bio.net/bionet/mm/biofilms/1998-September/ ...
Biofilm - Wikipedia
For other species in disease-associated biofilms and biofilms arising from eukaryotes see below. Biofilms have been found to be ... may contribute to biofilm dispersal. Enzymes that degrade the biofilm matrix may be useful as anti-biofilm agents. Evidence has ... Documentary on Biofilms: The Silent Role of Biofilms in Chronic Disease HD Video Interviews on biofilms, antibiotics, etc. with ... biofilms can grow in showers very easily since they provide a moist and warm environment for the biofilm to thrive. Biofilms ...
biofilms | Lab Manager
The Mechanics of Biofilms- Sacrifice of the Few for the Benefit of the Many. April 12, 2016 ... New Anti-Biofilm Compounds Show Promise Against Drug-Resistant Bacteria Linked to Hospital Infections. February 17, 2016 ... Researchers in China report that air plasma can be used to kill biofilms found on the surfaces of perishable fruits and foods ... New research has shown that a bacteriophage is critical to the formation of biofilms by Pseudomonas ...
Bacterial biofilms, begone
... especially when they are allowed to form biofilms. "Biofilms are nasty once they form, and incredibly difficult to get rid of ... In particular, theyre devising new ways to keep harmful bacteria from forming sticky matrices called biofilms - and to do it ... In the lab, they demonstrated an 85 percent reduction in P. aeruginosa biofilm adhesion. They conducted extensive studies ... have created a new material that inhibits biofilm formation of the virulent superbug Pseudomonas aeruginosa. Their material, ...
The Biofilm Primer | J. William Costerton | Springer
... the widely accepted hypothesis that the majority of bacteria in virtually all ecosystems grow in matrix-enclosed biofilms. The ... The Biofilm Primer. Authors. * J. William Costerton Series Title. Springer Series on Biofilms. Series Volume. 1. Copyright. ... In 2004, Bill was recruited by the University of Southern California to build a center for biofilms in the dental and medical ... J. William (Bill) Costerton directed the NSF-funded Center for Biofilm Engineering in Montana for more than a decade. ...
MicroorganismsRegulated biofilm formationInfectionsPseudomonasSurfaces2017AntibioticsMicrobiologyBacteriumPlanktonicStaphylococcusResearchersCenter for Biofilm EngineeringImaged the formation of biofilmsStudy of biofilmsAeruginosaSlimyMature biofilmsMicrobesFormation of a biofilmMicrobial cellsMulticellular communitiesResistantCandidaFormDispersalInfectious BiofilmsSlimeSessileFound in biofilms2018VirulenceOrganismsReactorAntibiotic ResistanceQuantified by confocal laser scanSectioning of microbial biofilmsClinically relevantStage of biofilm formationPrevent the formation of biofilmsDisruptionCommunitiesDental plaquePersistenceSpringer Series on BiofilmsPolysaccharideResistanceCathetersAnti-biofilm activitiesBacteria in the biofilmCells within the biofilmDegradationCystic fibrosisExtracellular matrixAntimicrobial Agents
Microorganisms32
- Microbial biofilms are multicellular communities of sessile microorganisms encased by the hydrated polymeric matrix. (springer.com)
- Once anchored to a surface, biofilm microorganisms carry out a variety of detrimental (like the plaque on teeth) or beneficial (bioremediating hazardous waste sites) reactions. (springer.com)
- Biofilms form on interior showerhead surfaces and potentially expose the user to a cohort of unknown, aerosolized microorganisms. (pnas.org)
- They grow wide and thick, forming a new, social dynamic among their member microorganisms, while also defending them: biofilms can be notoriously inaccessible to antibiotics, which is why they have drawn a lot of medical research. (eurekalert.org)
- Microphytobenthic biofilms in mudflats are characterised by a wide variety of microorganisms and the production of large quantities of extracellular polymeric substances (EPS). (springer.com)
- Microorganisms , or organisms too small to see with the naked eye , are the building blocks for biofilms. (howstuffworks.com)
- A biofilm is composed of living, reproducing microorganisms, such as bacteria, which exist as a colony, or community. (wikiversity.org)
- A biofilm forms when microorganisms adhere to the surface of some object in a moist environment and begin to reproduce. (wikiversity.org)
- Throughout our discussion, we provide detailed lists and summaries of some of the most prominent and extensively researched dispersal agents that have shown promise against the biofilms of clinically relevant pathogens, and we catalog which specific microorganisms they have been shown to be effective against. (mdpi.com)
- Donlan, R. M. & Costerton, J. W. Biofilms: survival mechanisms of clinically relevant microorganisms. (nature.com)
- The findings will contribute towards a better understanding of the growth of microorganisms in space, which is also key to bioregenerative life support systems, the formation of biofilms and microbial ore extraction. (lifeboat.com)
- A biofilm is a structured community of microorganisms on a surface, encapsulated in a self-formed matrix made of extracellular polymeric substances (EPS). (lifeboat.com)
- This EPS matrix holds the microorganisms together in their three-dimensional arrangement and enables the biofilm to adhere to surfaces. (lifeboat.com)
- The properties of microorganisms living within a biofilm generally differ substantially from those of microorganisms of the same species existing independently. (lifeboat.com)
- This means that microorganisms in biofilms are highly resistant to various chemical and physical effects and can be used for a range of applications in space. (lifeboat.com)
- A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
- Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
- The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
- A biofilm is formed when numerous living microorganisms, such as bacteria or fungi, group together and then evolve and grow as a collective. (lboro.ac.uk)
- However, beneficial applications in which biofilms can be exploited also exist - for example, ' Bioremediation' , a cost-effective eco-friendly process of degrading harmful pollutants from the air, soil, and water into non-toxic substances using microorganisms. (lboro.ac.uk)
- Figure caption: Most microorganisms do not float individually in water but live together in organised communities called biofilms. (lboro.ac.uk)
- Many microorganisms can naturally grow together on surfaces to form complex aggregations called biofilms. (wikipedia.org)
- One distinctive characteristic of biofilm formation is that microorganisms within biofilms are often much tougher and more recalcitrant compared to individuals. (wikipedia.org)
- This phenomenon of enhanced resistance can potentially be beneficial in industrial chemical production, where microorganisms within biofilms may tolerate higher chemical concentration and act as robust manufacturing "factories" for various products. (wikipedia.org)
- Biofilm-forming microorganisms are characterized by high invasiveness, the ability to cause dangerous and difficult to treat infections. (intechopen.com)
- Biofilm, also called as the biological membrane, is a complex, multicellular, and multifunctional structure of one or more species of microorganisms, surrounded by a layer of organic and inorganic substances produced by these microorganisms adhering to both biotic and abiotic surfaces. (intechopen.com)
- Biofilm microorganisms have been associated with several human diseases, including valve endocarditis - an infection that settles in the heart - and cystic fibrosis - the biofilm protects the bacteria that can cause this disease. (cleanlink.com)
- Biofilms are dense aggregates of surface-adherent microorganisms embedded in an exopolysaccharide matrix. (jci.org)
- A biofilm community can be formed by a single bacterial species, but in nature biofilms almost always consist of rich mixtures of many species of bacteria, as well as fungi, algae, yeasts, protozoa, other microorganisms, debris and corrosion products. (montana.edu)
- The formulation uses multiple chemical and physical actions to penetrate a biofilm, kill the microorganisms, and remove the biofilm from surfaces. (thepoultrysite.com)
- A biofilm is an aggregate of microorganisms in which cells adhere to each other on a surface. (medicalxpress.com)
- Biofilms are a consortium of microorganisms and extracellular substances in association with a solid surface in contact with liquid. (kenyon.edu)
Regulated biofilm formation1
- To test whether the structural binary switch regulated biofilm formation, the authors analysed biofilm development and architecture for each of the mutants. (nature.com)
Infections51
- While based in microbiology, biofilms are of intense interest to many other scientists, because they affect whole ecosystems, and lie at the root of chronic bacterial infections. (springer.com)
- Led by Dianne Newman , the Gordon M. Binder/Amgen Professor of Biology and Geobiology, the group identified a protein that degrades and inhibits biofilms of Pseudomonas aeruginosa , the primary pathogen in cystic fibrosis (CF) infections. (caltech.edu)
- While there is precedent for the use of enzymes to treat bacterial infections, the novelty of this study lies in our observation that selectively degrading a small pigment that supports the biofilm lifestyle can inhibit biofilm expansion," says Costa, the first author on the study. (caltech.edu)
- While it will take several years of experimentation to determine whether the laboratory findings can be translated to a clinical context, the work has promise for the utilization of proteins like PodA to treat antibiotic-resistant biofilm infections, the researchers say. (caltech.edu)
- The C auris biofilm is not as strong as those of some other clinically significant fungi, but has nonetheless contributed to C auris ' ability to survive in hospital environments and cause outbreaks and infections. (medscape.com)
- So, when faced with biofilm infections, what can doctors do? (medicalnewstoday.com)
- 60% of microbial infections, and 80% of chronic infections in humans, finding ways to disrupt established biofilms is of critical importance. (prweb.com)
- Concepts for the prevention of obstinate polymer-associated infections include the search for new anti-infectives active in biofilms and new biocompatible materials that complicate biofilm formation and the development of vaccines. (nih.gov)
- Whether in oceans, lung infections skin, gut microbiota - the physics of fluids are ubiquitous to biofilms," says Rossy. (eurekalert.org)
- Many infections contracted in hospital are linked to the formation of biofilms. (uclouvain.be)
- Then they multiply and form what are called biofilms, multicellular communities that can cause nosocomial infections that are especially difficult to treat because biofilms make their bacteria even more resistant to antibiotics. (uclouvain.be)
- The equipment damage, product contamination, energy losses, and medical infections caused by biofilms cost the United States billions of dollars every year. (medicalnewstoday.com)
- Three recent articles [ 1 , 2 , 3 ] highlight how our understanding of bacterial persistence and recent advances in disinfection modalities may provide a means for control of biofilms and nosocomial infections. (medscape.com)
- Biofilm infections normally blocked by a healthy person's immune system on Earth may pose problems to astronauts in space, said Cynthia Collins, a professor at Rensselaer involved in the study. (howstuffworks.com)
- She said examining biofilm growth beyond Earth's gravity can lead to better treatment for astronauts who may be at higher risks of developing biofilm-related infections while away from Earth. (howstuffworks.com)
- Many biofilms are quite harmful (for example, when they produce infections in wounds) and must be treated or controlled. (wikiversity.org)
- Biofilms have been found to be involved in a wide variety of microbial infections in the body. (wikiversity.org)
- Over 60% of all microbial infections are caused by biofilms. (wikiversity.org)
- Biofilms have been implicated in such common problems as urinary tract infections, catheter infections, middle-ear infections, formation of dental plaque, gingivitis and coating contact lenses [5] . (wikiversity.org)
- While acute infections can be removed after a short treatment of antibiotics, biofilm infections usually are never completely eliminated, resulting in recurrent episodes. (wikiversity.org)
- Biofilm-associated infections pose a complex problem to the medical community, in that residence within the protection of a biofilm affords pathogens greatly increased tolerances to antibiotics and antimicrobials, as well as protection from the host immune response. (mdpi.com)
- Since as much as 80% of human bacterial infections are biofilm-associated, many researchers have begun investigating therapies that specifically target the biofilm architecture, thereby dispersing the microbial cells into their more vulnerable, planktonic mode of life. (mdpi.com)
- Biofilms are often detrimental, with biofilm infections estimated to be responsible for up to 80% of all infections in humans and animals . (lboro.ac.uk)
- Gaining a greater understanding of the composition of biofilms and how to prevent, detect, manage and engineer them, would present benefits across a range of sectors, particularly in the healthcare industry where managing infections effectively is a growing issue globally. (lboro.ac.uk)
- Biofilm-related infections are among the top four leading causes of death in hospitals so everything researchers can learn about the early phases of a biofilm could become a tool to eradicate them. (lboro.ac.uk)
- Figuring out a way to prevent the bacteria from forming biofilms could make infections easier to treat. (newscientist.com)
- Biofilms, which are resistant to antibiotics and disinfectants, corrode pipes and cause diseases such as lung infections, but they can be used beneficially to treat sewage, industrial waste, and contaminated soil. (thefreedictionary.com)
- By severely curtailing the effects of antibiotics, the formation of organized communities of bacterial cells known as biofilms can be deadly during surgeries and in urinary tract infections. (phys.org)
- Bacterial biofilm infections are particularly problematic because sessile bacteria can withstand host immune responses and are drastically more resistant to antibiotics, biocides and hydrodynamic shear forces than their planktonic counterparts. (frontiersin.org)
- Treatment of biofilm-associated infections is a field that requires further study, in part due to the high levels of antibiotic resistance exhibited by biofilm structures conferred in part by the exopolysaccharide matrix. (frontiersin.org)
- Several serious diseases are caused by biofilm-associated Staphylococcus aureus , infections in which the accessory gene regulator ( agr ) quorum-sensing system is thought to play an important role. (asm.org)
- Many of these diseases, including endocarditis, osteomyelitis, and foreign-body related infections, appear to be caused by biofilm-associated S. aureus ( 12 , 18 , 30 , and 44 ). (asm.org)
- These properties likely contribute to the persistence and recalcitrance to treatment of staphylococcal biofilm infections. (asm.org)
- The agr system contributes to virulence in model biofilm-associated infections, including endocarditis ( 7 , 50 ) and osteomyelitis ( 3 , 15 ), although the precise role of the agr system varies with the type of infection model used ( 16 , 17 , 54 ). (asm.org)
- This has arisen, in part, because of the estimate by the Centers for Disease Control and Prevention that 65% of human bacterial infections involve biofilms. (jci.org)
- Enterococcus faecalis is a common cause of health care-associated infections in humans, largely due to its ability to persist in the hospital environment, colonize patients, acquire antimicrobial resistance, and form biofilms. (asm.org)
- Biofilms cost the U.S. literally billions of dollars every year in energy losses, equipment damage, product contamination and medical infections. (montana.edu)
- Respiratory and related structures provide ideal environments for the development of bacterial biofilms, which predispose patients to recurrent and chronic infections. (scribd.com)
- Biofilms are important for the persistence of chronic rhinosinusitis, pulmonary infections in cystic fibrosis, chronic otitis media, and device-related infections. (scribd.com)
- Understanding the nature of biofilms in common pediatric infections is essential to comprehending the expected course of bacterial illness and identifying treatments that are most likely to be beneficial against more resistant biofilms. (scribd.com)
- Indeed, infectious disease experts at the Centers for Disease Control and Prevention (CDC) estimate that 65% of human bacterial infections involve biofilms. (sciencemag.org)
- Such biofilms often lead to infections of the bladder or other organs. (sciencemag.org)
- Since then, biofilms have been implicated in numerous infections. (sciencemag.org)
- Biofilms, Infection, and Antimicrobial Therapy provides an in-depth exploration of biofilms, offering broad background information, as well a detailed look at the serious concerns to which biofilm-associated infections give rise. (waterstones.com)
- Prosthetic device infections, such as those involving artificial heart valves, intravascular catheters, or prosthetic joints, are prime examples of biofilm-associated infections. (waterstones.com)
- As biofilm-associated infections are particularly difficult to treat, they result in significant mortality, morbidity, and increased economic burden. (waterstones.com)
- They explore the molecular mechanisms of bacterial adherence, biofilm formation, regulation of biofilm maintenance, and cell-to-cell communication and present the latest information on various treatment protocols that should aid physicians in the treatment of these refractory and often difficult-to-treat infections. (waterstones.com)
- Other biofilms are "bad" when they cause fouling and infections of humans and plants. (iwapublishing.com)
- Formation of these sessile communities and their inherent resistance to antibiotics and host immune attack are at the root of many persistent and chronic bacterial infections ( 1 ), including those caused by Pseudomonas aeruginosa , which has been intensively studied as a model for biofilm formation ( 2 , 3 ). (sciencemag.org)
- In a clinical rabbit model of contact lens-related P. aeruginosa infection, our scientists have shown that the use of polyaspartic acid disrupt pathogenic biofilms formed on contact lenses and reduce corneal infections. (nationaljewish.org)
- Using the state grant to identify the most effective charged poly(amino acids) at disrupting biofilms and testing such compounds on infected contact lenses, and in animal models of eye and skin infections. (nationaljewish.org)
Pseudomonas18
- Jennings LK, Storek KM, Ledvina HE, Coulon C, Marmont LS, Sadovskaya I et al (2015) Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix. (springer.com)
- Researchers in the laboratory of Melissa Reynolds, associate professor in the Department of Chemistry and core faculty in the School of Biomedical Engineering, have created a new material that inhibits biofilm formation of the virulent superbug Pseudomonas aeruginosa. (colostate.edu)
- Aizenberg and her collaborators reported that SLIPS reduced by 96% the formation of three of the most notorious, disease-causing biofilms-Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus-over a 7-day period. (innovations-report.com)
- Moreover, this book also provides a comprehensive account on microbial interactions in biofilms, pyocyanin, and extracellular DNA in facilitating Pseudomonas aeruginosa biofilm formation, atomic force microscopic studies of biofilms, and biofilms in beverage industry. (intechopen.com)
- Biofilm supported batches of the isolated Pseudomonas species were able to degrade crude oil much readily and extensively than the planktonic counterparts. (mendeley.com)
- We have recently reported the synthesis of a small molecule, denoted TAGE, based on the natural product bromoageliferin and demonstrated that TAGE has anti-biofilm activity against Pseudomonas aeruginosa. (rti.org)
- In this study we looked at the effect of three different sets of Fe 3 O 4 nanoparticles (FeNPs) on the development of Pseudomonas aeruginosa PAO1 biofilms. (scirp.org)
- C. Haney, J. Rowe and J. Robinson, "Spions Increase Biofilm Formation by Pseudomonas aeruginosa ," Journal of Biomaterials and Nanobiotechnology , Vol. 3 No. 4A, 2012, pp. 508-518. (scirp.org)
- Quorum sensing in Pseudomonas aeruginosa biofilms. (scirp.org)
- 2005. Iron salts perturb biofilm formation and disrupt existing biofilms of Pseudomonas aeru-ginosa. (scirp.org)
- 2008. Influence of quorum sensing and iron on twitching motility and biofilm formation in Pseudomonas aeruginosa. (scirp.org)
- 2009. Iron-binding compounds impair Pseudomonas aeruginosa biofilm formation, especially under anaerobic conditions. (scirp.org)
- 2007. Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. (scirp.org)
- 2005. Iron availability influences aggregation, biofilm, adhesion and invasion of Pseudomonas aeruginosa and Burkholderia cenocepacia. (scirp.org)
- More recent studies by Nels Hoiby of the University of Copenhagen in Denmark show that biofilms containing the bacterium Pseudomonas aeruginosa clog the lungs of 80% to 90% of these patients. (sciencemag.org)
- Enhanced Pseudomonas Aeruginosa Biofilm Development Mediated by Human Neutrophils. (nationaljewish.org)
- Neutrophil Enhancement of Pseudomonas Aeruginosa Biofilm Development: Human F-actin and DNA as Targets for Therapy. (nationaljewish.org)
- Disruption of Contact Lens-Associated Pseudomonas Aeruginosa Biofilms Formed in the Presence of Neutrophils. (nationaljewish.org)
Surfaces37
- Outside of the laboratory, most microbes grow as organised biofilm communities on surfaces. (nih.gov)
- Biofilms are densely packed communities of microbial cells that grow on surfaces and surround themselves with secreted polymers. (nih.gov)
- The C auris strains developed 1.5 to 3 times less biofilm mass than C albicans , but 3 to 6 times greater mass than C glabrata , suggesting C auris biofilm has enough strength to adhere to hospital surfaces and instruments. (medscape.com)
- Found all over the planet - from desert rocks to the surfaces of buildings - biofilms are an integral part of nature. (medicalnewstoday.com)
- As described in Methods and Materials , biofilms were obtained by swab of interior surfaces of 45 showerheads from nine cities in the United States. (pnas.org)
- the cells are biofilm and haemagglutination negative, less virulent and less adhesive on hydrophilic surfaces. (nih.gov)
- Bacteria form biofilms by attaching to each other on a wide variety of surfaces: the bottom of oceans, lakes or rivers, medical equipment and even internal organs, like the intestine, lungs, and teeth - the latter is the familiar dental plaque, a large source of income for dentists. (eurekalert.org)
- She chose two different clones of the bacterium Caulobacter crescentus , which is commonly found in freshwater lakes and streams, and undergoes a "stalked" cell stage that allows it to anchor on surfaces, colonize them, and form biofilms. (eurekalert.org)
- Biofilms form when bacteria stick to surfaces and establish communities held together by a slimy, glue-like substance made of sugary molecular strands that they excrete to form an extracellular matrix. (medicalnewstoday.com)
- Biofilms grow on all kinds of surfaces, both natural and man-made. (medicalnewstoday.com)
- In medicine, biofilms are a hazard because they can form on living surfaces, such as heart and lung tissue, and on medical devices and implants. (medicalnewstoday.com)
- Biofilms are sessile microbial communities growing on surfaces, frequently embedded in a matrix of extracellular polymeric substances (also known as slime). (medscape.com)
- These findings are consistent with a tendency of other Acinetobacter species to survive on dry surfaces, form biofilms, and contribute to nosocomial outbreaks. (medscape.com)
- Various bacteria, including foodborne pathogens, can form biofilms on stainless steel surfaces, potentially leading to contamination of foods. (uga.edu)
- Researchers in China report that air plasma can be used to kill biofilms found on the surfaces of perishable fruits and foods. (labmanager.com)
- People have tried all sorts of things to deter biofilm build-up-textured surfaces, chemical coatings, and antibiotics, for example," says Aizenberg, Amy Smith Berylson Professor of Materials Science at the Harvard School of Engineering and Applied Sciences (SEAS) and a Core Faculty Member at the Wyss Institute for Biologically Inspired Engineering at Harvard. (innovations-report.com)
- The tenacity with which biofilms adhere to surfaces is the property which makes cleaning difficult. (wikiversity.org)
- Biofilms may form on living or non-living surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
- Given the chance, the bacteria will spread across surfaces such as human tissue to form a biofilm that may resist treatment with antibiotics. (newscientist.com)
- Microbes are known to colonize surfaces and form biofilms. (mdpi.com)
- Naturally occurring or synthetic antimicrobial peptides have been shown to prevent microbial colonization of surfaces, to kill bacteria in biofilms and to disrupt the biofilm structure. (mdpi.com)
- strains are characterized by their ability to form a biofilm structure on biotic and abiotic surfaces, causing significant problems in many industrial branches and threatening human health. (intechopen.com)
- Biofilm is most commonly formed on solid surfaces staying in contact with water, living tissues, and liquid-air interface. (intechopen.com)
- The attachment of planktonic cells to surfaces is the first and most crucial step in biofilm formation. (asm.org)
- Biofilm forms when bacteria adhere to surfaces in moist environments by excreting a slimy, glue-like substance. (montana.edu)
- Sites for biofilm formation include all kinds of surfaces: natural materials above and below ground, metals, plastics, medical implant materials-even plant and body tissue. (montana.edu)
- The Effects of Sugars on the Biofilm Formation of Escherichia coli 185p on Stainless Steel and Polyethylene Terephthalate Surfaces in a Laboratory Model. (pearltrees.com)
- Once Salmonella bacteria get into a food processing facility and have an opportunity to form a biofilm on surfaces, it is likely to be extraordinarily difficult, if not impossible, to kill it, according to research published ahead of print in the journal Applied and Environmental Microbiology. (innovations-report.com)
- Researchers from National University of Ireland, Galway conducted a study in which they attempted to kill Salmonella biofilms on a variety of hard surfaces, using three types of disinfectant. (innovations-report.com)
- This image shows photos of biofilms on surfaces at low magnification (300) and high magnification (3000) using electron microscope. (innovations-report.com)
- We found that all of the types of Salmonella we looked at were able to adopt the specialized biofilm lifestyle on all of the surfaces we looked at, including glass, stainless steel, glazed tile, and plastic, and that the biofilm of Salmonella gets more dense over time, and becomes more firmly attached to the surface," she says. (innovations-report.com)
- Biofilms are protective layers of proteins and polysaccharides that surround bacteria and stick to equipment surfaces. (thepoultrysite.com)
- Evidence supporting the idea that bacteria attach themselves to surfaces throughout the body began appearing about a decade later when Thomas Marrie of Dalhousie University in Halifax, Nova Scotia, using the then recently developed scanning electron microscope, detected a biofilm coating a heart pacemaker removed from a patient. (sciencemag.org)
- On the other hand, biofilms forming on food-contact surfaces can lead to hygienic problems and economical losses due to food spoilage. (kenyon.edu)
- Biofilm formation can occur on any submerged surfaces in any environment with the present of bacteria. (kenyon.edu)
- The ability of bacteria to attach to surfaces and to form biofilms often is an important competitive advantage for them over bacteria growing in suspension. (iwapublishing.com)
- Researchers have discovered that exposing polycarbonate polymer films to beams of iron ions can prevent biofilms of microbial cells forming on the film surfaces 1 . (natureasia.com)
20173
- Humphries J, Xiong L, Liu J, Prindle A, Yuan F, Arjes HA et al (2017) Species-independent attraction to biofilms through electrical signaling. (springer.com)
- Cite this: Chlorhexidine Effective Against Candida auris Biofilms - Medscape - Jan 12, 2017. (medscape.com)
- Martyn Dade-Robertson, Alona Keren-Paz, Meng Zhang and Ilana Kolodkin-Gal (2017) Architects of nature: growing buildings with bacterial biofilms. (weizmann.ac.il)
Antibiotics19
- Many infectious pathogens are difficult to treat because they develop into biofilms, layers of metabolically active but slowly growing bacteria embedded in a protective layer of slime, which are inherently more resistant to antibiotics. (caltech.edu)
- biofilms tolerate conventional antibiotics much better than other modes of bacterial growth. (caltech.edu)
- The protective dome of a biofilm shields bacteria from antibiotics. (medicalnewstoday.com)
- Interestingly, antibiotics can actually enter a biofilm in many instances, but the EPS actively shields its inhabitants from such compounds, promoting antibiotic resistance. (medicalnewstoday.com)
- Such treatments include surgically removing the area that is infected with a biofilm or using high-velocity water sprays to physically disrupt the biofilm, often in combination with antibiotics. (medicalnewstoday.com)
- Biofilms are surface-attached communities of bacterial or fungal cells that are enmeshed in an extensive extracellular matrix which makes them more resistant to both antibiotics and the immune system. (prweb.com)
- This is because the bacteria in the biofilm can be up to 1,000 times more resistant to antibiotics than the same bacteria grown in liquid medium. (wikiversity.org)
- Biofilms -- communities of bacteria in self-produced slime -- pose a major challenge in healthcare because of their extraordinary resistance to antibiotics. (eurekalert.org)
- Microbes form a biofilm in response to a number of different factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. (wikipedia.org)
- The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
- Three other aminoglycoside antibiotics also stimulated biofilm formation. (sciencemag.org)
- Thus, the authors conclude that biofilm formation is a defensive response to aminoglycoside antibiotics that involves changes in bacterial c-di-GMP content. (sciencemag.org)
- Interestingly, the Marshall Protocol does just that - although it uses pulsed, low-dose antibiotics - which have been shown to effectively destroy biofilms - and Benicar to get the job done with more vigor than a flossing addict could ever achieve. (bacteriality.com)
- Biofilms result in microbial resistance so that the microbes are resistant to prescription antibiotics and antifungals. (rainbow.coop)
- The form of biofilm enhances the effectiveness of microbial protection against the adverse environmental factors, including antibiotics, reduces the effectiveness of host defense mechanisms, facilitates the acquisition of nutrients, creates the possibility of horizontal gene transfer by providing evolutionary and genetic diversity, and enables the transmission of information between microbial cells [ 1 , 2 , 3 ]. (intechopen.com)
- They're Tough: The bacteria within biofilm are as much as 1,000 times more resistant to antibiotics and disinfectants than bacteria found outside of biofilm. (cleanlink.com)
- Bacteria sequestered in biofilms are shielded from attack by the host's immune system and are often much harder to kill with antibiotics than their free-floating or "planktonic" counterparts, says William Costerton, director of the Center for Biofilm Engineering at Montana State University in Bozeman. (sciencemag.org)
- An Institut Pasteur-CNRS research team has characterized a Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics. (medicalxpress.com)
- Objectives: To investigate the development of mutational resistance to antibiotics in staphylococcal biofilms. (ebscohost.com)
Microbiology11
- Biofilms are one of the most widely distributed and successful modes of life on Earth," says Prof. Hans-Curt Flemming - director of the Institute for Interface Biotechnology at the University of Duisburg-Essen in Germany - in a 2016 article published in Nature Reviews Microbiology . (medicalnewstoday.com)
- Citing that the techniques currently used to identify anti-biofilm activities in phage-derived proteins have the "important shortcomings" of being laborious endpoint assays that suffer from poor reproducibility, in the recent issue of Frontiers in Microbiology a team of scientists lead by Diana Gutierrez have reported a proof of concept study using an xCELLigence Real-Time Cell Analysis instrument to monitor the disruption of clinically important Staphylococcus aureus biofilms. (prweb.com)
- The author, who proposed this biofilm hypothesis, uses direct evidence from microscopy and from molecular techniques, presenting cogent reasons for moving beyond conventional culture methods that dominated microbiology throughout the last century. (springer.com)
- Bacteria grow predominantly in biofilms in all natural, engineered, and pathogenic ecosystems, and this book provides a solid basis for the understanding of bacterial processes in environmental, industrial, agricultural, dental and medical microbiology. (springer.com)
- Bill is widely regarded as the "grandfather" of biofilm microbiology. (springer.com)
- Please go to URL http://www.bio.net and click on the 'Access the BIOSCI/bionet Newsgroups' option and then click on the BIOFILMS/bionet.microbiology.biofilms hyperlink. (bio.net)
- If you do not see bionet.microbiology.biofilms in your newsreader within another day or two, ask your news system administrator to act on our 'newgroup' message to enable the group at your site. (bio.net)
- You might also try the command 'g bionet.microbiology.biofilms' in rn-like newsreaders. (bio.net)
- The study of biofilms represents a new way of understanding the microbiology of virtually everything around us, from problems which affect industries to serious public health issues. (wikiversity.org)
- The complexity of biofilm activity and behavior requires research contributions from many disciplines such as biochemistry, engineering, mathematics and microbiology. (montana.edu)
- Biofilms require a coordinated attack by researchers with expertise in everything from microbiology and immunology to materials science and mathematical modeling, Mangan explains. (sciencemag.org)
Bacterium7
- From this soil, Costa isolated a bacterium that disrupts biofilms. (caltech.edu)
- They measured the bacterial and tissue responses to the implants after infecting the animals with Staphylococcus aureus , a common bacterium found in biofilms. (medicalnewstoday.com)
- A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
- Joao Xavier from the Memorial Sloan-Kettering Cancer Center in New York and colleagues placed colonies of the bacterium at the centre of nutrient plates and let them spread to form such biofilms. (newscientist.com)
- The key ingredient in the new material is biofilm produced by the bacterium Bacillus subtilis. (tum.de)
- The culprit turned out to be chunks of biofilm containing the bacterium Legionella pneumoniae that had wafted out of air conditioners. (sciencemag.org)
- The scientists tested the anti-biofilm properties of ion-treated polymer films and compared them with those of untreated polymer films by growing Salmonella typhi , a disease-causing bacterium, on both films. (natureasia.com)
Planktonic12
- Enclosed in a matrix primarily consisting of polysaccharides, pathogens in biofilms are often more resistant to environmental stresses such as heat and chemical sanitizers than are their planktonic counterparts. (uga.edu)
- Biofilms are defined as sessile communities characterized by cells that are irreversibly attached to a surface or to each other, embedded in a matrix of extracellular polymeric substances (EPS), and that exhibit differential phenotypic characteristics with respect to their planktonic counterparts [2] . (wikiversity.org)
- Biofilm is a multidisciplinary, gold open access journal focused on hypothesis- or discovery-driven studies on microbial cells that grow in multicellular communities (including surface-attached biofilms and suspended aggregates) and demonstrate different gene expression, growth rate, behavior and appearance to those that are in planktonic (free-living) state. (elsevier.com)
- The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single-cells that may float or swim in a liquid medium. (wikipedia.org)
- Candida biofilm is a heterogeneous, spatially well-organized structure consisting of planktonic and mycelial yeast forms which are interdependent in the quorum sensing system and surrounded by an extracellular polysaccharide substance. (intechopen.com)
- Furthermore, the cells in the biofilm, compared to planktonic forms, show reduced sensitivity to chemical compounds with antifungal activity and increased survival under unfavorable environmental conditions. (intechopen.com)
- The study of bacteria residing in biofilms as an interactive community rather than free-living planktonic cells has recently gained a great deal of attention. (jci.org)
- For instance, microbial biofilms are naturally tolerant of antibiotic doses up to 1,000 times greater than doses that kill planktonic bacteria. (montana.edu)
- Bacteria can grow as free-floating, planktonic bacteria or complex communities called biofilms. (scribd.com)
- Antimicrobial therapy that is proven effective against planktonic bacteria is often insufficiently effective against the defenses of biofilms. (scribd.com)
- Rather than existing in a planktonic or free-living form, evidence indicates that microbes show a preference for living in a sessile form within complex communities called biofilms. (waterstones.com)
- Methods: Mutation frequencies to resistance against mupirocin and rifampicin were determined for planktonic cultures and for biofilms generated using either a novel static biofilm model. (ebscohost.com)
Staphylococcus3
- Iwase T, Uehara Y, Shinji H, Tajima A, Seo H, Takada K et al (2010) Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization. (springer.com)
- Zinc-dependent mechanical properties of Staphylococcus aureus biofilm-forming surface protein SasG', PNAS, 2015. (uclouvain.be)
- In all, at least a dozen proteins are responsible for staphylococcus aureus biofilm formation. (uclouvain.be)
Researchers22
- Now, a group of researchers at Caltech and the University of Oxford have made progress in the fight against biofilms. (caltech.edu)
- To better understand the fungus' biofilm capabilities, the researchers compared the biofilm mass of four strains of C auris with that of two other fungi, Candida albicans and Candida glabrata . (medscape.com)
- Researchers have developed a new biomaterial that creates a "slippery surface" and stops infectious bacterial biofilms forming on implanted medical devices. (medicalnewstoday.com)
- The researchers, including a team from the Wyss Institute at Harvard University in Boston, MA, report their findings in the journal Biofilms . (medicalnewstoday.com)
- In future studies, the researchers aim to better understand the mechanisms involved in preventing biofilms. (innovations-report.com)
- For years, researchers have focused on developing antimicrobial treatments to stop the formation of biofilms, but as the statistics indicate, they have yet to find an affordable, effective solution. (infectioncontroltoday.com)
- A team of researchers with members from the U.S., Germany and Sweden has discovered that the cellulose found in bacterial biofilms differs from the cellulose in plants. (phys.org)
- The researchers assume that the added biofilm stimulates uniform crystal growth throughout the volume of the hybrid material. (tum.de)
- We want researchers to know that we recognize the importance of biofilms and [want to] bring people together to work on the problem," says Dennis Mangan of the National Institute of Dental and Craniofacial Research (NIDCR), who is spearheading the effort. (sciencemag.org)
- The goal is to understand how and why biofilms form so that researchers can identify their Achilles' heel and devise better treatments, which are badly needed. (sciencemag.org)
- The first inklings that biofilms could be a health problem came in the mid-1960s when dental researchers Johannes Van Houte and Ronald Gibbons of the Forsyth Dental Center in Boston, Massachusetts, recognized that bacteria living in the mouth synthesize gummy adhesives that accumulate on the teeth, gums, and tongue. (sciencemag.org)
- Leading an international team of researchers in creating simulations of how fluids move, Mathijssen, a postdoctoral scholar in bioengineering at Stanford, found that individual bacteria and biofilms can generate currents strong enough to draw in distant nutrients. (stanford.edu)
- In their work , published Dec. 11 in Physical Review Letters , the researchers were able to find predictable patterns of how fluids move based on the general shapes of biofilms, insights that could find applications in many fields. (stanford.edu)
- Within organized biofilms, the researchers found two common patterns of movement: vortexes and asters. (stanford.edu)
- The researchers were able to combine vortex and aster patterns within a single biofilm to determine how the bacteria would push, pull and whirl the fluids around them. (stanford.edu)
- Mathematical Modeling of Biofilms gives a state-of-the-art overview that is especially valuable for educating students, new biofilm researchers, and design engineers. (iwapublishing.com)
- The report provides the foundation for researchers seeking to use biofilm modeling or to develop new biofilm models. (iwapublishing.com)
- By targeting a noninfectious viral cage to biofilms, researchers developed a system for early detection and eventual destruction of these bacterial communities. (ebscohost.com)
- Researchers at National Jewish Health have determined that actin originating from necrotized human neutrophils serve as a biological matrix in the formation of microbial biofilms in the airways of cystic fibrosis (CF) patients. (nationaljewish.org)
- Researchers at MIT have identified new polymers found in mucus, known as mucins, that can provide protection against biofilm formation, the slimy layers of bacterial growth that are very difficult to remove, by trapping the bacteria and preventing them from clumping together on a surface. (medindia.net)
- To probe the anti-biofilm properties of iron ions, the researchers exposed polycarbonate films to iron-ion beams of two different energies. (natureasia.com)
- The nanopores on the polycarbonate surface prevented the bacteria from forming biofilms by generating bacteria-repelling effects such as repulsive, electrostatic and acid-base forces, the researchers say. (natureasia.com)
Center for Biofilm Engineering1
- J. William (Bill) Costerton directed the NSF-funded Center for Biofilm Engineering in Montana for more than a decade. (springer.com)
Imaged the formation of biofilms1
- Rossy imaged the formation of biofilms at the level of single bacteria to monitor the effects of each flow rate on the bacterial colonies. (eurekalert.org)
Study of biofilms2
- The study of biofilms has increased rapidly in recent years as we have become more aware of their pervasiveness and impact. (medicalnewstoday.com)
- The study of biofilms has skyrocketed in recent years due to increased awareness of the pervasiveness and impact of biofilms on natural and industrial systems, as well as human health. (montana.edu)
Aeruginosa13
- Our research suggests a new approach to inhibiting P. aeruginosa biofilms. (caltech.edu)
- Adding PodA to growing cultures of P. aeruginosa , the team discovered, inhibits biofilm development. (caltech.edu)
- In the lab, they demonstrated an 85 percent reduction in P. aeruginosa biofilm adhesion. (colostate.edu)
- Low concentrations of tobramycin, which failed to inhibit the growth rate or alter global protein synthesis, induced the formation of biofilms (adherent communities of bacteria that contribute to antibiotic resistance and persistent infection) in P. aeruginosa and Escherichia coli . (sciencemag.org)
- Herein we demonstrate that TAGE: (1) does not have selective toxicity against cells within the biofilm state, (2) will inhibit biofilm development under flow conditions, indicating that the CV staining protocol correlates with the ability to be active under biomimetic conditions, and (3) will disperse preformed P. aeruginosa biofilms. (rti.org)
- Advanced derivatives of TAGE have generated compounds shown to be exceedingly effective as biofilm inhibitors against the g-proteobacteria in this study (P. aeruginosa strains PAO1, PA14, PDO300, and Acinetobacter baumannii). (rti.org)
- For example, a quorum-sensing-defective mutant of P. aeruginosa is unable to form the highly differentiated biofilm structure associated with wild-type P. aeruginosa , at least under certain conditions ( 11 ). (asm.org)
- IODOSORB has been shown to disrupt and substantially eradicate mature biofilms of P. aeruginosa ( in-vitro ). (smith-nephew.com)
- During studies of alginate biosynthesis in P. aeruginosa , we discovered that the majority of the extracellular material that reacted in the carbazole colorimetric assay was not exopolysaccharide but DNA [as determined by its peak absorbance at 260 nm, by electrophoretic display, and by its deoxyribonuclease (DNase) but not ribonuclease sensitivity] and therefore hypothesized that this DNA may play a functional role in P. aeruginosa biofilms. (sciencemag.org)
- Four flow-chamber channels were inoculated with green fluorescent protein (GFP)-tagged P. aeruginosa PAO1, and two channels each were irrigated with minimal medium with or without DNase I. The presence of DNase I in the medium prevented biofilm formation. (sciencemag.org)
- To this end, we inoculated five flow-chamber channels and irrigated them with minimal medium without DNase I to allow the establishment of P. aeruginosa biofilms of varying age. (sciencemag.org)
- These results indicate that extracellular DNA is required for the initial establishment of P. aeruginosa biofilms and perhaps biofilms formed by other bacteria that specifically release DNA. (sciencemag.org)
- Our scientists have developed charged poly amino acids that promotes the depolymerization of actin and reduced biofilm development of P. aeruginosa in vitro. (nationaljewish.org)
Slimy4
- Read on to understand why starting a biofilm is a slimy affair. (howstuffworks.com)
- If someone has ever walked in a stream or river, they may have slipped on rocks that were slimy with biofilm. (wikiversity.org)
- These include bacterial biofilms, such as dental plaque and the slimy black coating that forms in sewage pipes. (tum.de)
- Until recently, the slimy conglomerations of bacteria known as biofilms were recognized mostly for their propensity to coat-and corrode-pipes. (sciencemag.org)
Mature biofilms5
- We have also begun to appreciate how cells can co-ordinate their activities and build the complex structures of mature biofilms that afford protection for their inhabitants. (nih.gov)
- Antimicrobial peptides (AMPs) have been used to control the formation and to eradicate mature biofilms. (mdpi.com)
- have found that d -amino acids found in conditioned medium from mature biofilms of Bacillus subtilis prevent biofilm formation and trigger existing biofilm disassembly. (sciencemag.org)
- Mature biofilms are very destructive to the teeth and surrounding gums, and many other varieties can actually provoke other significant medical health problems as well. (colgate.com)
- This suggests that the matrix in mature biofilms may be strengthened by other substances or that mature biofilms may produce sufficient proteolytic exoenzymes to locally inactivate the DNase I ( 7 ). (sciencemag.org)
Microbes9
- Previous microbiological studies of showerhead biofilms have used culture methodology to detect and identify microbes, and have focused primarily on Legionella pneumophilia ( 7 , 8 , 9 ) and Mycobacterium avium ( 10 - 12 ). (pnas.org)
- As illustrated in the micrographs in Fig. 1 , microbes generally were clumped and embedded in extracellular material, consistent with biofilm morphology. (pnas.org)
- A key factor in the microbes' success on mudflats is that they live together in neighborhoods: the biofilms. (scientificamerican.com)
- These biofilms are communities of microbes encased in a self-produced matrix that often contains polysaccharides, DNA and proteins. (mdpi.com)
- Oral diseases, including caries and periodontitis, consist of biofilms* produced by microbes. (rainbow.coop)
- The study of factors that allow manipulation of biofilm microbes in the biofilm growth state requires that reproducible biofilms by generated. (unt.edu)
- And when pathogenic microbes form biofilms, they are. (medicalxpress.com)
- Biofilms appear to afford microbes a survival advantage by optimizing nutrition, offering protection against hostile elements, and providing a network for cell-to-cell signaling and genetic exchange. (waterstones.com)
- Unfortunately, one of the most troubling characteristics of microbes found in biofilms is a profound resistance to antimicrobial agents. (waterstones.com)
Formation of a biofilm1
- In this microscopy image, some bacteria (bright particles) are shown as they take the first steps in the formation of a biofilm. (lboro.ac.uk)
Microbial cells2
- Biofilm is a multidisciplinary, gold open access journal focused on hypothesis- or discovery-driven studies on microbial cells that grow in multicellular communities (including surface-attached biofilms and suspended aggregates) and demonstrate different gene expression, growth rate, behavior and appearance. (elsevier.com)
- Through collaborations with experimentalists, the group are trying to understand the early phase in the life of a biofilm, that is, the transition from the very beginning, when there is no biofilm and simply some microbial cells are swimming in a fluid, to the phase when the cells find a surface to colonise, and start reproducing on it. (lboro.ac.uk)
Multicellular communities1
- Most bacteria can form complex, matrix-containing multicellular communities known as biofilms, which protect residents from environmental stresses such as antibiotic exposure. (sciencemag.org)
Resistant6
- Biofilms are tricky beasts because they have a tendency to become resistant to all manner of efforts employed to eradicate them. (medicalnewstoday.com)
- Biofilm residence also affords the bacteria with antibiotic-resistant genes the ability to easily share these among their neighbors using a process called horizontal gene transfer . (medicalnewstoday.com)
- A cell sorter will enable us to tackle the problem of biofilm resistance by allowing us to separate resistant and virulent bacteria from the general population and determine what genetic and regulatory mechanisms these bacteria use to survive and grow within the host," said David Davies, an associate professor of biological sciences at Binghamton. (eurekalert.org)
- Chuanwu Xi, PhD, Assistant Professor in the Department of Environmental Health at the University of Michigan and his research team found that treated steam from a novel steam disinfection system utilizing TANCS® technology rapidly kills highly resistant biofilms with greater than 99.95 % killing efficiency in a 3 second treatment, and to a non-detectable level in a less than 10 second treatment. (prweb.com)
- Was it better at forming a dense biofilm or was it more resistant to disinfectants than other Salmonella? (innovations-report.com)
- This form of graphene is extremely resistant to biofilm formation, which has promise for places like water-treatment plants, oil-drilling operations, hospitals and ocean applications like underwater pipes that are sensitive to fouling," Tour said in a statement. (theengineer.co.uk)
Candida4
- Onder andere met FISH, een genetische analysetechniek, bracht Buijssen de architectuur van de biofilm en interactie tussen Candida en lactobacillen gedetailleerd in kaart. (rug.nl)
- Fulvic Acid and Candida Albicans Biofilms. (rainbow.coop)
- Presence of Extracellular DNA in the Candida albicans Biofilm Matrix and its Contribution to Biofilms. (ebscohost.com)
- In Candida albicans, there is a scarce knowledge concerning the contribution of extracellular DNA (eDNA) to biofilm matrix and overall structure. (ebscohost.com)
Form31
- Many bacterial species form biofilms, and their study has revealed them to be complex and diverse. (nih.gov)
- The study showed that C auris can form a biofilm that resists many antifungal agents. (medscape.com)
- Although unable to form biofilms equivalent to C. albicans , C. auris has a noteworthy virulence capacity that merits further exploration," report Leighann Sherry, PhD, from the University of Glasgow in Scotland, and colleagues. (medscape.com)
- When bacteria flock together and form a community, this is called a biofilm. (medicalnewstoday.com)
- The ability to form a biofilm affords at least two properties: the adherence of cells to a surface and accumulation to form multilayered cell clusters. (nih.gov)
- About ninety percent of bacterial life at the surface of Earth is found in the form of biofilms" he says. (eurekalert.org)
- Little by little, these bacteria form a bacterial community that becomes a biofilm. (uclouvain.be)
- Bravo and colleagues [ 1 ] investigated the ability of Acinetobacter pittii strains to form biofilms after long-term desiccation if they were fed with nutrient media. (medscape.com)
- Despite the reduction in culturability over time, all strains that recovered from starvation on solid-surface experiments retained their capacity to form biofilms after rehydration, an addition of nutrients, and changing temperature. (medscape.com)
- Mutants that locked RbmA in the closed O-loop dimer state were substantially defective in biofilm formation, whereas mutants that were locked in the monomeric D-loop state were able to form biofilms that closely resembled wild-type biofilms, which suggests that the switch regulates biofilm development. (nature.com)
- The authors propose a model in which the switch and oligomerization state of a single component of the V. cholerae biofilm matrix (RbmA) determines the architecture and plasticity of the biofilm by influencing its ability to form higher-order structures with VPS. (nature.com)
- Bella Neufeld, the first author and graduate student who led the research, explained that her passion for finding new ways to fight superbugs is motivated by how adaptive and impenetrable they are, especially when they are allowed to form biofilms. (colostate.edu)
- Biofilms are nasty once they form, and incredibly difficult to get rid of," Neufeld said. (colostate.edu)
- As a result, biofilms cannot cling to the material, and even if they do form, they easily 'slip' off under mild flow conditions," adds Wong, a researcher at SEAS and a Croucher Foundation Postdoctoral Fellow at the Wyss Institute. (innovations-report.com)
- Microbial biofilms are widely present in nature where this kind of growth is the usual form of bacterial growth . (wikiversity.org)
- Biofilms are a form of collective life with emergent properties that confer many advantages on their inhabitants, and they represent a much higher level of organization than single cells do. (nature.com)
- Biofilms: an emergent form of microbial life. (nature.com)
- Biofilms can form on the teeth of most animals as dental plaque, where they may cause tooth decay and gum disease. (wikipedia.org)
- Hydrophobicity can also affect the ability of bacteria to form biofilms. (wikipedia.org)
- They form in pipes, in water systems, on living organisms-the first biofilm discovered was on a person's teeth-and, of course, on mud. (scientificamerican.com)
- One form of the present invention is a fabricated biofilm storage device with a biologic material applied to a substrate to form, e.g., a dry thin film stable at room temperature for extended periods of time. (freepatentsonline.com)
- Another form of the present invention is a method of fabricating a biofilm storage device in which a biologic material is applied to a substrate under conditions that promote alignment of the biologic material on the substrate. (freepatentsonline.com)
- But although growing several flagella allowed the bacteria to efficiently cover the entire surface, the adaptation also left them less able to stick together tightly and form biofilms. (newscientist.com)
- According to the US Navy's Office of Naval Research, micro-fouling in the form of adhesive surface biofilms can increase drag by up to 20%, while the macro-fouling caused by larger organisms - typically barnacles - can add more than 60% overall. (naval-technology.com)
- Generally, they form organized, settled structures taking the form of multicellular clusters forming biofilm. (intechopen.com)
- Many Streptococci use quorum-sensing systems to regulate several physiological properties, including the ability to incorporate foreign DNA, tolerate acid, form biofilms, and become virulent. (jci.org)
- People need to question whether disinfectants that are promoted as killing various types of bacteria are really as effective in real life situations where biofilms can form as they are claimed to be based on experiments that do not use biofilms. (innovations-report.com)
- Under threat of being scrubbed away with disinfectant, individual bacteria can improve their odds of survival by joining together to form colonies, called biofilms. (stanford.edu)
- Biofilms can be beneficial or detrimental to the environment on which they form. (kenyon.edu)
- Over 90% of bacterial biomass exists in the form of biofilms. (iwapublishing.com)
- Dr. Duraj-Thatte reviews the means that the bacteria she works with can be used for creating biofilms that can have rigid form and could be used to create synthetic biological building materials. (harvard.edu)
Dispersal6
- Dispersal: finally, after biofilms have reached maturity, they can propagate through detachment of small or large clumps of cells, which allows bacteria to attach to a surface or to a biofilm away from the original community. (wikiversity.org)
- This review addresses the current state of research into medical biofilm dispersal. (mdpi.com)
- Lastly, we discuss some of the main hurdles to development of biofilm dispersal agents, and contemplate what needs to be done to overcome them. (mdpi.com)
- In this Research Topic, we aim to highlight groundbreaking work in the development and/or application of quantitative analysis methods to bacterial biofilms, with a focus on those studies resulting in the discovery of new phenomena regulating biofilm initiation, development, function, or dispersal. (frontiersin.org)
- This includes cell motility within biofilm and biofilms dispersal. (weizmann.ac.il)
- 3. Biofilms can propagate through detachment of small or large clumps of cells, or by a type of 'seeding dispersal' that releases individual cells. (montana.edu)
Infectious Biofilms1
- The ability to attach and implant devices in the human body is crucial in modern medicine but a big problem with their use is that they provide a surface for bacteria to colonize on and turn into infectious biofilms. (lboro.ac.uk)
Slime3
- Many years of research on biofilm and migratory shorebirds at Roberts Bank and other areas illustrate the covert yet vital role that the slime plays in the ecosystem. (scientificamerican.com)
- Without realising it, you actually see biofilms every day - examples include dental plaque, slime on rocks in streams, and pond scum. (lboro.ac.uk)
- Biofilm EPS, which is also referred to as slime (although not everything described as slime is a biofilm), is a polymeric conglomeration generally composed of extracellular DNA, proteins, and polysaccharides. (medicalxpress.com)
Sessile1
- Biofilms are sessile microbial communities embedded in a self-produced extracellular polymeric matrix ( 12 , 44 ). (asm.org)
Found in biofilms1
- The Biofilm mode of growth provides such significant advantages to the members of the consortium that most organisms in important habitats are found in biofilms. (unt.edu)
20182
- Cite this: Survival of the Slimiest: The Persistent Nature of Biofilms - Medscape - Mar 22, 2018. (medscape.com)
- 2018. "Action of Antimicrobial Peptides against Bacterial Biofilms. (mdpi.com)
Virulence1
- Once the cells "sense" that a quorum has been reached, they begin to communicate, a process that in turn "throws the switch" for manufacturing virulence factors, such as biofilms. (scienceblog.com)
Organisms5
- The structural and physiological complexity of biofilms has led to the idea that they are coordinated and cooperative groups, analogous to multicellular organisms. (nih.gov)
- 3. The method of claim 1 wherein the step of providing a biofilm layer comprises introducing a gas into the gas compartment that diffuses through the membrane and selects gas degrading organisms in the biofilm layer. (google.com)
- The engineers of mudflat biofilms are single-celled photosynthesizing organisms called diatoms. (scientificamerican.com)
- While classically bacteria are thought of as unicellular organisms in nature they exist in complex "multicellular" states - biofilms. (weizmann.ac.il)
- Since biofilm formation allows for the survival of microbial organisms in the airways and is also associated with increased morbidity and mortality, targeting actin could be the basis for the development of a potential therapy for CF. (nationaljewish.org)
Reactor7
- Numerous challenges call for novel solutions and concepts of analytics, biofilm reactor design, product recovery, and scale-up strategies. (nih.gov)
- In this review, we highlight recent advancements in spatiotemporal biofilm characterization and new biofilm reactor developments for the production of value-added fine chemicals as well as current challenges and future scenarios. (nih.gov)
- MBBR (Moving bed biofilm reactor) is now the common name for biological treatment process invented by Professor Halvard Odegaard in Trondheim in the late eighties. (environmental-expert.com)
- The Moving Bed Biofilm Reactor (MBBR) is one of the most advanced wastewater treatment technologies available in the world today as it combines the benefits of both the suspended and the attached growth process. (environmental-expert.com)
- The TBRâ„¢ is an advanced MBBRâ„¢ biofilm technology is based on specially designed plastic biofilm carriers or biocarriers that are suspended and in continuous movement within a tank or reactor of specified volume. (environmental-expert.com)
- The Moving Bed Biofilm Reactor or MBBR process is based on the aerobic biofilm principle and utilizes activated sludge and other biofilm systems. (environmental-expert.com)
- Such limitation of bioavailability can be overcome by steady-state biofilm-based reactor. (mendeley.com)
Antibiotic Resistance2
- By disrupting the communication process, he explained, the new compound could lead to drugs that will prevent the formation of biofilms, restoring the potency of antibiotic treatments and limiting the development of antibiotic resistance. (scienceblog.com)
- 2010. Antibiotic resistance of bacterial biofilms. (scirp.org)
Quantified by confocal laser scan1
- Biodegradation of crude oil was estimated by gas chromatography, and biofilm formation near oil-water interface was quantified by confocal laser scanning microscopy. (mendeley.com)
Sectioning of microbial biofilms1
- Optical sectioning of microbial biofilms. (asm.org)
Clinically relevant2
- The 7th ASM Conference on Biofilms will provide a platform to discuss the latest research, covering topics that include molecular basis and regulation of biofilm formation, biofilms in natural and industrial systems, diagnosis and study of clinically-relevant biofilms, and emerging technologies and their application to biofilms. (asm.org)
- Biofilm Formation: A Clinically Relevant Microbiological Process. (ebscohost.com)
Stage of biofilm formation2
- The final stage of biofilm formation is known as dispersion, and is the stage in which the biofilm is established and may only change in shape and size. (wikipedia.org)
- This stage of biofilm formation is likely to be mediated in part by cell wall-associated adhesins, including the microbial surface components recognizing adhesive matrix molecules. (asm.org)
Prevent the formation of biofilms1
- To date, this may be the first successful test of a nontoxic synthetic surface that can almost completely prevent the formation of biofilms over an extended period of time. (innovations-report.com)
Disruption4
- Importantly, this real-time biofilm disruption data correlated well with the data from traditional end point assays such as the labor intensive crystal violet staining technique. (prweb.com)
- Under some conditions, disruption of agr expression had no discernible influence on biofilm formation, while under others it either inhibited or enhanced biofilm formation. (asm.org)
- Stage 2 requires disruption with a toothbrush, such as a Colgate® Enamel Health™ 360® Toothbrush , and floss to dislodge the attachment created by the biofilm. (colgate.com)
- Continuous and regular disruption of these biofilms is imperative for prevention and management of oral diseases. (ebscohost.com)
Communities17
- By measuring the biofilm growing rates under different evenness levels of communities, an evenly distributed community favors the formation of biofilms was observed. (springer.com)
- But why are biofilms so persistent, and what are doctors and scientists doing to outsmart these clever microbial communities? (medicalnewstoday.com)
- Biofilms can be made up of populations of the same bacteria or of communities, which, in turn, are made up of many different species, all living together under a protective dome. (medicalnewstoday.com)
- In much the same way as human communities, biofilms have highly specialized areas: some of them are responsible for nutrient recycling, while others produce new EPS components or send messages from one area of the biofilm to another. (medicalnewstoday.com)
- Although this also happens in free-living bacterial communities, it is significantly more efficient in biofilms. (medicalnewstoday.com)
- With this knowledge we attempt to engineer desirable biofilm communities on specific materials. (eawag.ch)
- Biofilm" aims to bring together different communities to significantly advance the knowledge of microbial communities, and specifically explore the interfaces between these disciplines. (elsevier.com)
- Biofilms are structurally, phenotypically, and compositionally diverse bacterial communities. (frontiersin.org)
- Biofilms, surface-associated microbial communities composed of aggregates of cells that are encased by an extracellular matrix, have a tremendous impact on industrial, clinical, and natural environments. (asm.org)
- Biofilms are currently defined as structured bacterial communities embedded in a self-produced exopolysaccharide matrix adherent to any abiotic or biological surface. (frontiersin.org)
- Biofilms are dynamic communities of bacteria and fungi living within a protective self-secreted matrix of sugars and proteins. (smith-nephew.com)
- Biofilm communities can develop within hours. (montana.edu)
- It was not until the late decades of the 20th century, however, that scientists and engineers possessed adequate technology to effectively study microbial communities and began to understand the significant implications of the biofilm mode of growth. (montana.edu)
- Here, we test the hypothesis that human colon biofilms comprise microbial communities that are carcinogenic in CRC mouse models. (jci.org)
- Remarkably, biofilm-positive communities from healthy colonoscopy biopsies induced colon inflammation and tumors similarly to biofilm-positive tumor tissues. (jci.org)
- 16S rRNA gene sequencing and RNA-Seq analyses identified compositional and functional microbiota differences between mice colonized with biofilm-positive and biofilm-negative communities. (jci.org)
- Bacterial biofilms are structured communities of cells enclosed in self-produced hydrated polymeric matrix adherent to an inert or living surface ( 1 ). (sciencemag.org)
Dental plaque4
- For example, over 500 bacterial species have been identified in typical dental plaque biofilms [4] . (wikiversity.org)
- Then, the link between biofilms and dental plaque and calculus was discovered. (colgate.com)
- however, the relationship between the pathogenic state and the biofilm mode of growth has been most clearly established with the oral streptococci, which are known to initiate dental caries when the bacteria are living in the biofilm environment of dental plaque. (jci.org)
- Dental plaque is biofilm. (montana.edu)
Persistence2
- Alfa and colleagues [ 2 ] looked at the impact of improper positioning of the elevator lever of a duodenoscope on bacterial persistence, biofilm formation, and endoscope contamination. (medscape.com)
- In Biofilms, Infection, and Antimicrobial Therapy, Drs Pace, Rupp, and Finch assemble the contributions of more than 50 of the world's leading authorities on microbial biofilms who present recent findings on antibacterial tolerance and bacterial persistence associated with biofilms and discuses the implications of those findings with regard to human health. (waterstones.com)
Springer Series on Biofilms1
- Springer Series on Biofilms presents carefully refereed volumes on selected topics on this field of research. (springer.com)
Polysaccharide2
- A number of biofilm-negative mutants have been isolated in which polysaccharide intercellular adhesin (PIA) production appears to be unaffected. (nih.gov)
- Polysaccharide matrices typically enclose bacterial biofilms. (wikipedia.org)
Resistance10
- Biofilms are central to our most important global challenges, from antimicrobial resistance and food safety to water security, and they have a significant economic, social, and environmental impact. (lboro.ac.uk)
- Although resistance has been a problem with bacteria which produce biofilms (to protect themselves from antimicrobials) resistance of bacteria to manuka honey has not been demonstrated. (rainbow.coop)
- Fungal Biofilm Resistance. (rainbow.coop)
- One of the most important advantages a biofilm status gives the bacteria is an increased antimicrobial resistance phenotype. (frontiersin.org)
- The chapter focuses on the emergence of antifungal resistance with the development of biofilms. (intechopen.com)
- 2015 Nov;3(11): 3300-3307 Differential biofilm formation and chemical disinfection resistance of Escherichia coli on stainless steel and polystyrene tissue culture plate. (pearltrees.com)
- January 2005 Biofilm Formation by Escherichia coli O157:H7 on Stainless Steel: Effect of Exopolysaccharide and Curli Production on Its Resistance to Chlorine. (pearltrees.com)
- Biofilms promote bacterial growth and diversity and offer bacteria unique environments, including aerobic and anaerobic layers, that facilitate resistance to antimicrobial therapies. (scribd.com)
- Furthermore, biofilms modify themselves following exposure to antimicrobial therapy, thus developing increased resistance. (scribd.com)
- Platelets Enhance Biofilm Formation and Resistance of Endocarditis-Inducing Streptococci on the Injured Heart Valve. (ebscohost.com)
Catheters5
- Biofilms are found naturally in many places, including teeth (plaque), medical devices such as intravenous catheters, prosthetic heart valves, and cardiac pacemakers, in sewage pipelines, ship hulls, and many other non-human environments. (google.com)
- This spells bad news for anyone with conditions such as cystic fibrosis , periodontitis , or chronic wounds as medical implants and catheters are hotspots for biofilm formation. (medicalnewstoday.com)
- Biofilms stick to just about everything, from copper pipes to steel ship hulls to glass catheters. (innovations-report.com)
- An artistic configuration of catheters in various stages of being clogged by biofilm. (infectioncontroltoday.com)
- About half of the time, the interior of long-term urinary catheters become plagued by biofilms-structures formed by colonies of bacteria that are extremely difficult to kill. (infectioncontroltoday.com)
Anti-biofilm activities1
- Studies that investigated anti-biofilm activities without describing the possible mechanisms were removed from the analysis. (mdpi.com)
Bacteria in the biofilm1
- 5. The method of claim 4 wherein the step of introducing the gas comprises introducing a gas comprising methane in sufficient amounts to promote growth of methylotrophic bacteria in the biofilm layer. (google.com)
Cells within the biofilm2
- The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides, proteins, lipids and DNA. (wikipedia.org)
- Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)
Degradation4
- Pyocyanin has been used in the clinical identification of this strain for over a century, but several years ago the Newman group demonstrated that the molecule also supports biofilm growth, raising the possibility that its degradation might offer a new route to inhibit biofilm development. (caltech.edu)
- Within the proprietary xCELLigence microtiter plates that contain gold biosensors, biofilms of S. aureus were established and then exposed to different bacteriophage-derived proteins that catalyze degradation of the key biofilm extracellular polymers peptidoglycan or exopolysaccharide. (prweb.com)
- The authors demonstrated that the degradation of these polymers, and the concomitant dissipation of the biofilm, causes a decrease in the xCELLigence biosensor signal that is both time- and dose-dependent. (prweb.com)
- This therapy, focused on biofilm prevention or degradation, is particularly applicable for early stage CF in young patients when antimicrobial agents are only partially effective at best. (nationaljewish.org)
Cystic fibrosis3
- And in the mid-1980s, Joseph Lam of the University of Calgary in Alberta, using the transmission electron microscope, confirmed that biofilms are present in the lungs of cystic fibrosis patients. (sciencemag.org)
- Antibiotic [therapy] kills some cells, but biofilms hunkered down survive the onslaught," says Peter Greenberg of the Cystic Fibrosis Research Center at the University of Iowa, Iowa City. (sciencemag.org)
- Targeted therapy for preventing or reducing biofilm formation in cystic fibrosis, cystitis, dental caries, wounds, and acne etc. (nationaljewish.org)
Extracellular matrix3
- V. cholerae biofilms consist of an extracellular matrix that is composed of Vibrio exopolysaccharides (VPS), lipids, nucleic acids and matrix proteins, including RbmA. (nature.com)
- Bacterial biofilms are defined as a community of surface-attached bacteria that are protected by an extracellular matrix of biomolecules. (rti.org)
- DNA has been described as a structural component of the extracellular matrix (ECM) in bacterial biofilms. (ebscohost.com)
Antimicrobial Agents1
- Effects of Antimicrobial Agents on an ( in-vitro ) Biofilm Model of Skin Wounds. (smith-nephew.com)