Biocatalysis
Biotechnology
Enzymes, Immobilized
Chemical Engineering
Enzymes
Catalysis
Biodegradation, Environmental
Hexanes
Solvents
Biotransformation
Stereoisomerism
Protein Engineering
Databases, Factual
Escherichia coli
Substrate Specificity
A modular treatment of molecular traffic through the active site of cholinesterase. (1/2123)
We present a model for the molecular traffic of ligands, substrates, and products through the active site of cholinesterases (ChEs). First, we describe a common treatment of the diffusion to a buried active site of cationic and neutral species. We then explain the specificity of ChEs for cationic ligands and substrates by introducing two additional components to this common treatment. The first module is a surface trap for cationic species at the entrance to the active-site gorge that operates through local, short-range electrostatic interactions and is independent of ionic strength. The second module is an ionic-strength-dependent steering mechanism generated by long-range electrostatic interactions arising from the overall distribution of charges in ChEs. Our calculations show that diffusion of charged ligands relative to neutral isosteric analogs is enhanced approximately 10-fold by the surface trap, while electrostatic steering contributes only a 1.5- to 2-fold rate enhancement at physiological salt concentration. We model clearance of cationic products from the active-site gorge as analogous to the escape of a particle from a one-dimensional well in the presence of a linear electrostatic potential. We evaluate the potential inside the gorge and provide evidence that while contributing to the steering of cationic species toward the active site, it does not appreciably retard their clearance. This optimal fine-tuning of global and local electrostatic interactions endows ChEs with maximum catalytic efficiency and specificity for a positively charged substrate, while at the same time not hindering clearance of the positively charged products. (+info)Quantum catalysis in enzymes: beyond the transition state theory paradigm. A Discussion Meeting held at the Royal Society on 14 and 15 November 2005. (2/2123)
How do enzymes work? What is the physical basis of the phenomenal rate enhancements achieved by enzymes? Do we have a theoretical framework that accounts for observed catalytic rates? These are the foremost questions-with particular emphasis on tunnelling phenomena-debated at this Discussion Meeting by the leading practitioners in the field. (+info)Genome-wide studies of histone demethylation catalysed by the fission yeast homologues of mammalian LSD1. (3/2123)
In order to gain a more global view of the activity of histone demethylases, we report here genome-wide studies of the fission yeast SWIRM and polyamine oxidase (PAO) domain homologues of mammalian LSD1. Consistent with previous work we find that the two S. pombe proteins, which we name Swm1 and Swm2 (after SWIRM1 and SWIRM2), associate together in a complex. However, we find that this complex specifically demethylates lysine 9 in histone H3 (H3K9) and both up- and down-regulates expression of different groups of genes. Using chromatin-immunoprecipitation, to isolate fragments of chromatin containing either H3K4me2 or H3K9me2, and DNA microarray analysis (ChIP-chip), we have studied genome-wide changes in patterns of histone methylation, and their correlation with gene expression, upon deletion of the swm1(+) gene. Using hyper-geometric probability comparisons we uncover genetic links between lysine-specific demethylases, the histone deacetylase Clr6, and the chromatin remodeller Hrp1. The data presented here demonstrate that in fission yeast the SWIRM/PAO domain proteins Swm1 and Swm2 are associated in complexes that can remove methyl groups from lysine 9 methylated histone H3. In vitro, we show that bacterially expressed Swm1 also possesses lysine 9 demethylase activity. In vivo, loss of Swm1 increases the global levels of both H3K9me2 and H3K4me2. A significant accumulation of H3K4me2 is observed at genes that are up-regulated in a swm1 deletion strain. In addition, H3K9me2 accumulates at some genes known to be direct Swm1/2 targets that are down-regulated in the swm1Delta strain. The in vivo data indicate that Swm1 acts in concert with the HDAC Clr6 and the chromatin remodeller Hrp1 to repress gene expression. In addition, our in vitro analyses suggest that the H3K9 demethylase activity requires an unidentified post-translational modification to allow it to act. Thus, our results highlight complex interactions between histone demethylase, deacetylase and chromatin remodelling activities in the regulation of gene expression. (+info)Functional effects of single nucleotide polymorphisms in the coding region of human N-acetyltransferase 1. (4/2123)
Genetic variants of human N-acetyltransferase 1 (NAT1) are associated with cancer and birth defects. N- and O-acetyltransferase catalytic activities, Michaelis-Menten kinetic constants (K(m) and V(max)) and steady-state expression levels of NAT1-specific mRNA and protein were determined for the reference NAT1*4 and variant human NAT1 haplotypes possessing single nucleotide polymorphisms (SNPs) in the open reading frame. Although none of the SNPs caused a significant effect on steady-state levels of NAT1-specific mRNA, C97T(R33stop), C190T(R64W), C559T (R187stop) and A752T(D251V) each reduced NAT1 protein level and/or N- and O-acetyltransferase catalytic activities to levels below detection. G560A(R187Q) substantially reduced NAT1 protein level and catalytic activities and increased substrate K(m). The G445A(V149I), G459A(synonymous) and T640G(S214A) haplotype present in NAT1*11 significantly (P<0.05) increased NAT1 protein level and catalytic activity. Neither T21G(synonymous), T402C(synonymous), A613G(M205V), T777C(synonymous), G781A(E261K) nor A787G(I263V) significantly affected K(m), catalytic activity, mRNA or protein level. These results suggest heterogeneity among slow NAT1 acetylator phenotypes. (+info)Light-activated deoxyguanosine: photochemical regulation of peroxidase activity. (5/2123)
(+info)An albumin-butyrylcholinesterase for cocaine toxicity and addiction: catalytic and pharmacokinetic properties. (6/2123)
(+info)Molecular characterization of propionyllysines in non-histone proteins. (7/2123)
(+info)Optimization of labile esters for esterase-assisted accumulation of nitroxides into cells: a model for in vivo EPR imaging. (8/2123)
(+info)Biocatalysis is the use of enzymes or other biological molecules to catalyze chemical reactions in a biological system. In the medical field, biocatalysis is often used to produce drugs, vaccines, and other therapeutic agents. Enzymes are proteins that act as biological catalysts, and they can be used to speed up chemical reactions that would otherwise occur slowly or not at all. Biocatalysis can also be used to modify or degrade biological molecules, such as DNA or proteins, in order to treat diseases or disorders. Biocatalysis has many advantages over traditional chemical synthesis methods, including higher selectivity, milder reaction conditions, and lower costs.
Biotechnology is the application of biological processes, organisms, or systems to develop or improve products, processes, and systems for various purposes, including medical applications. In the medical field, biotechnology is used to develop new drugs, vaccines, diagnostic tools, and medical devices. Some examples of biotechnology applications in medicine include: 1. Genetic engineering: This involves manipulating genes to create new organisms or modify existing ones to produce useful products, such as insulin for diabetes treatment. 2. Gene therapy: This involves using genetic material to treat or prevent diseases by replacing or repairing faulty genes. 3. Proteomics: This involves studying the structure and function of proteins, which play a crucial role in many biological processes, including disease. 4. Biopharmaceuticals: These are drugs or other therapeutic agents that are produced using living cells or organisms, such as monoclonal antibodies. 5. Diagnostic tools: Biotechnology is used to develop diagnostic tools, such as PCR (polymerase chain reaction) tests, that can detect the presence of specific genes or proteins in biological samples. Overall, biotechnology has revolutionized the medical field by enabling the development of new and more effective treatments for a wide range of diseases and conditions.
Enzymes, immobilized, refer to enzymes that have been chemically or physically attached to a solid support, such as a plastic or glass surface, or encapsulated within a matrix. This immobilization allows the enzymes to be used repeatedly in a variety of applications, such as in industrial processes, environmental remediation, and medical diagnostics. In the medical field, immobilized enzymes are used in a variety of diagnostic tests and therapeutic applications. For example, they can be used to detect specific molecules in biological samples, such as glucose in blood or proteins in urine. They can also be used to catalyze specific chemical reactions, such as the conversion of one molecule into another, which can be useful in drug development and production. One advantage of immobilized enzymes is that they can be easily separated from the reaction mixture and reused, which can reduce costs and increase efficiency. Additionally, immobilization can protect the enzymes from degradation or denaturation, which can improve their stability and activity over time.
Chemical engineering is not typically used in the medical field in the way it is used in other industries. Chemical engineering is a branch of engineering that deals with the design, development, and operation of processes that convert raw materials into useful products, such as chemicals, fuels, and food. In the medical field, chemical engineers may be involved in the development of new medical devices or the optimization of existing processes for the production of pharmaceuticals or medical equipment. However, the primary focus of chemical engineering in the medical field is on the development of new drugs and medical treatments, rather than on the production of these treatments.
Enzymes are biological molecules that act as catalysts in various chemical reactions within living organisms. They are proteins that speed up chemical reactions by lowering the activation energy required for the reaction to occur. Enzymes are essential for many bodily functions, including digestion, metabolism, and DNA replication. In the medical field, enzymes are used in a variety of ways. For example, they are used in diagnostic tests to detect the presence of certain diseases or conditions. They are also used in the treatment of certain medical conditions, such as digestive disorders, where the deficiency or malfunction of specific enzymes can cause symptoms. Enzyme replacement therapy is a type of treatment that involves replacing missing or defective enzymes in individuals with certain genetic disorders, such as Gaucher disease or Fabry disease. Enzyme inhibitors are also used in the treatment of certain medical conditions, such as hypertension and diabetes, by blocking the activity of specific enzymes that contribute to the development of these conditions. Overall, enzymes play a crucial role in many aspects of human health and are an important area of research in the medical field.
In the medical field, catalysis refers to the acceleration of a chemical reaction by a catalyst. A catalyst is a substance that increases the rate of a chemical reaction without being consumed or altered in the process. Catalysts are commonly used in medical research and drug development to speed up the synthesis of compounds or to optimize the efficiency of chemical reactions. For example, enzymes are biological catalysts that play a crucial role in many metabolic processes in the body. In medical research, enzymes are often used as catalysts to speed up the synthesis of drugs or to optimize the efficiency of chemical reactions involved in drug metabolism. Catalysis is also used in medical imaging techniques, such as magnetic resonance imaging (MRI), where contrast agents are used to enhance the visibility of certain tissues or organs. These contrast agents are often synthesized using catalytic reactions to increase their efficiency and effectiveness. Overall, catalysis plays a critical role in many areas of medical research and drug development, helping to accelerate the synthesis of compounds and optimize the efficiency of chemical reactions.
Biodegradation, Environmental in the medical field refers to the process by which microorganisms break down and consume organic matter in the environment. This process is important in the management of medical waste, as it helps to reduce the amount of waste that is sent to landfills and reduces the risk of environmental contamination. Biodegradation can occur naturally, through the action of microorganisms in the environment, or it can be accelerated through the use of biodegradable materials or biodegradation agents. In the medical field, biodegradation is often used to dispose of medical waste, such as bandages, gauze, and other materials that are contaminated with bodily fluids or other potentially infectious materials.
Hexanes are a group of organic compounds that consist of six carbon atoms bonded together in a chain. They are commonly used as solvents in various industries, including the medical field. In the medical field, hexanes are used as a solvent for the extraction of certain compounds from biological samples, such as blood or tissue. They are also used as a cleaning agent for medical equipment and as a component in some medical devices, such as inhalers. However, it is important to note that hexanes are toxic and can cause respiratory and neurological damage if inhaled or ingested in large quantities. Therefore, proper handling and disposal of hexanes are essential to prevent accidental exposure and ensure the safety of medical personnel and patients.
Biotransformation is a process in the body where foreign substances, such as drugs, toxins, and environmental chemicals, are converted into more water-soluble and easily excreted forms. This process occurs in the liver and involves various enzymes that modify the chemical structure of the substance, making it less toxic and more easily eliminated from the body. Biotransformation can occur through two main pathways: phase I and phase II. In phase I, enzymes called cytochrome P450 oxidize the substance, adding or removing hydrogen atoms, which can make the substance more reactive and potentially toxic. In phase II, enzymes such as glutathione S-transferases and UDP-glucuronosyltransferases add functional groups to the substance, making it more water-soluble and easier to excrete. Biotransformation is an important process in the body as it helps to detoxify harmful substances and prevent them from building up in the body. However, certain factors such as genetics, age, liver function, and certain medications can affect the rate and efficiency of biotransformation, which can impact the effectiveness and safety of drugs and other substances in the body.
In the medical field, "Databases, Factual" refers to electronic databases that contain factual information about medical topics, such as diseases, treatments, medications, and medical procedures. These databases are typically created and maintained by medical organizations, such as the National Library of Medicine (NLM) or the World Health Organization (WHO), and are used by healthcare professionals, researchers, and the general public to access and retrieve information about medical topics. Factual databases in the medical field may include information such as: * Descriptions of diseases and conditions, including symptoms, causes, and treatments * Information about medications, including dosage, side effects, and interactions with other drugs * Data on medical procedures, including risks, benefits, and outcomes * Research studies and clinical trials related to medical topics * Guidelines and recommendations from medical organizations and professional associations Factual databases in the medical field are often searchable and may include features such as filtering, sorting, and the ability to save and share search results. They are an important resource for healthcare professionals and researchers, as they provide access to a large and up-to-date collection of information on medical topics.
Recombinant proteins are proteins that are produced by genetically engineering bacteria, yeast, or other organisms to express a specific gene. These proteins are typically used in medical research and drug development because they can be produced in large quantities and are often more pure and consistent than proteins that are extracted from natural sources. Recombinant proteins can be used for a variety of purposes in medicine, including as diagnostic tools, therapeutic agents, and research tools. For example, recombinant versions of human proteins such as insulin, growth hormones, and clotting factors are used to treat a variety of medical conditions. Recombinant proteins can also be used to study the function of specific genes and proteins, which can help researchers understand the underlying causes of diseases and develop new treatments.
Biocatalysis
Biocatalysis & Biotransformation
Ben G. Davis
Nicotinamide
Nicotinonitrile
New Biotechnology
Phenylalanine ammonia-lyase
Cericlamine
Nocardioides
Arthrobacter crystallopoietes
Kitasatospora xanthocidica
Caldanaerovirga
Penicillium oxalicum
Marco Fraaije
Glycogen debranching enzyme
Solvolysis
Health effects of Bisphenol A
Arenobufagin
4-hydroxyacetophenone monooxygenase
Cytophagales
Paenarthrobacter nicotinovorans
Ureibacillus terrenus
Katja Loos
Penicillium miczynskii
Roger A. Sheldon
Monascus
Ribozyme
GIR1 branching ribozyme
Pleurotus eryngii
Melting curve analysis
Biosynthesis and Biocatalysis of Natural Products | Florida Atlantic University
INDUSTRIAL BIOCATALYSIS | Università degli studi dell'Insubria
Biocatalysis: Overcoming Equilibrium Issues with Carbonyl Reductase Enzymes - Almac
Continuous flow biocatalysis - Vapourtec
Biotechnology
Consultation Fermentation and biocatalysis consultation services
PhD position in Biocatalysis - Daily Trends
ESAB - European Society of Applied Biocatalysis - Membership
Biocatalysis in Organic Synthesis 3, Workbench Edition
Jan Deska - Synthetic Biocatalysis - University of Helsinki, Finland
'Publication' in iBB | Scoop.it
Geschützter Bereich - Login - Schulz laboratory Ruhr-Universität Bochum Biosynthesis Biocatalysis Naturstoffe
Biocatalysis as a Tool for the Synthetic Chemist - Scientific Update - UK
Diagnosis of a model of Duchenne muscular dystrophy in blood serum of mdx mice using Raman hyperspectroscopy | Scientific...
Cicily J. Ronhovde | Center for Biocatalysis and Bioprocessing | The University of Iowa
Biocatalysis by transglutaminases: a review of biotechnological applications - IRep - Nottingham Trent University
Chemical Engineering and Chemical Process Technology (eBook)
University of Iowa- Center for Biocatalysis and Bioprocessing (CBB): Read reviews and ask questions | Handshake
Biocatalysis for the production of pharmaceutical intermediates : statin precursors / Anera Švarc, Dino Skendrović, Ana...
Chemistry
Sean Murray | California State University, Northridge
Postdoc
Jobs.com - Search 1000s postdoctoral positions worldwide!
Search
Biomolecules | December 2013 - Browse Articles
Worldwide Atorvastatin Calcium Market Increasing Demand and Future Potential of Industry 2020-2029 - PharmiWeb.com
Biocatalytic designs for the conversion of renewable glycerol into glycerol carbonate as a value-added product
GRC Connects: Natural Products & Bioactive Compounds
AI for Learning, Teaching, and Writing - American Chemical Society
Flow biocatalysis1
- This new research post will focus on developing and employing biocatalysts, and their application in a wide range of formats (e.g., flow biocatalysis, cascade biocatalysis, single step biocatalysis, chemo-biocatalysis) for biomanufacturing. (futurebrh.com)
Enzymology2
- It is our pleasure to share our knowledge and skill on fermentation, biocatalysis, enzymology and microbiology. (indienz.com)
- Just let us know what you want and we provide you innovative solutions through consultation on fermentation, biocatalysis, enzymology and microbiology. (indienz.com)
Enzymes1
- Biocatalysis uses enzymes to replace traditional catalysts. (hotdailytrends.com)
Continuous1
- With continuous flow systems offering improved mixing, mass transfer, thermal control, pressurized processing, decreased variation, automation, process analytical technology, and in-line purification, the combination of biocatalysis and flow chemistry opens powerful new process windows. (vapourtec.com)
Chemists2
- These reasons, and especially the latter, are the major reasons why synthetic chemists have become interested in biocatalysis. (wikipedia.org)
- Chemists who want to learn how biocatalysis can help them. (scientificupdate.com)
Reaction2
- The use of biocatalysis to obtain enantiopure compounds can be divided into two different methods: Kinetic resolution of a racemic mixture Biocatalyzed asymmetric synthesis In kinetic resolution of a racemic mixture, the presence of a chiral object (the enzyme) converts one of the stereoisomers of the reactant into its product at a greater reaction rate than for the other reactant stereoisomer. (wikipedia.org)
- The organization is based on the type of reaction performed under biocatalysis. (thieme.in)
Fundamentals1
- Biocatalysis: Fundamentals and Applications, A. S. Bommarius and B. R. Riebel. (uninsubria.eu)
Protein1
- The ideal candidate for this project is an experimental chemist, with affinity or interest in biocatalysis, bioinformatics and protein engineering. (hotdailytrends.com)
Transformations3
- Biocatalysis underpins some of the oldest chemical transformations known to humans, for brewing predates recorded history. (wikipedia.org)
- More than one hundred years ago, biocatalysis was employed to do chemical transformations on non-natural man-made organic compounds, with the last 30 years seeing a substantial increase in the application of biocatalysis to produce fine chemicals, especially for the pharmaceutical industry. (wikipedia.org)
- Biocatalysis in Organic Synthesis offers critical reviews of organic transformations by experts, including experimental procedures. (thieme.in)
Technology2
- We have a proven track record of saving time and cost through the integration of our services and application of innovative biocatalysis and technology solutions. (almacgroup.com)
- Despite the growing impact of biocatalysis in industrial chemistry, the full potential of this technology is yet to be unlocked. (hotdailytrends.com)
Pharmaceutical1
- Biocatalysis for the production of pharmaceutical intermediates : statin precursors / Anera Švarc, Dino Skendrović, Ana Vrsalović Presečki. (nsk.hr)
Application2
- The final exam will start discussing a recent research paper or patent, selected by the candidate, describing an industrially relevant application of biocatalysis. (uninsubria.eu)
- Individuals from all over the world who have expertise and interest in the field of biocatalysis and its applications are invited to join ESAB and are welcome to submit online their application form to the ESAB Office. (esabweb.org)
Industrial2
- The aims of ESAB are to promote initiatives in areas of growing scientific and industrial interest of importance within the field of Applied Biocatalysis. (esabweb.org)
- The three Science of Synthesis volumes on Biocatalysis in Organic Synthesis present a broad contemporary overview on the state-of-the-art in enzymatic methods for asymmetric synthesis suitable for academics and industrial researchers working in the field of organic synthesis. (thieme.in)
Research2
- The research in my group focuses on the biosynthesis and biocatalysis of marine natural products, especially those with potential applications in medicine, veterinary medicine and agriculture. (fau.edu)
- Companies as well as academic, governmental, research and other public Institutions whose activities are related to the field of applied biocatalysis, are welcome to apply for Institutional Membership. (esabweb.org)
University1
- The project, entitled Biocatalysis for new products based on hemicellulose, includes researchers Henrik Stålbrand (project manager), Tommy Nylander, Patric Jannasch and Ola Wallberg from the Faculty of Science and the Faculty of Engineering at Lund University. (lu.se)
Chemical1
- Biocatalysis refers to the use of living (biological) systems or their parts to speed up (catalyze) chemical reactions. (wikipedia.org)
Catalysis2
- However, mechanistically speaking, biocatalysis is simply a special case of heterogeneous catalysis. (wikipedia.org)
- Applications that employ structural biology approaches to studying catalysis and biocatalysis are reviewed in MSFA. (nih.gov)
PHOTOCAT241
- PHOTOCAT24 is an international high education school for PhD students, post-docs and young researchers workign in the field of sustainable chemistry such as photochemistry and biocatalysis, both in academia and industry. (conference-service.com)
Synthetic1
- These reasons, and especially the latter, are the major reasons why synthetic chemists have become interested in biocatalysis. (wikipedia.org)
Enzyme1
- The use of biocatalysis to obtain enantiopure compounds can be divided into two different methods: Kinetic resolution of a racemic mixture Biocatalyzed asymmetric synthesis In kinetic resolution of a racemic mixture, the presence of a chiral object (the enzyme) converts one of the stereoisomers of the reactant into its product at a greater reaction rate than for the other reactant stereoisomer. (wikipedia.org)
Synthesis1
- With potent herbicidal activity, biocatalysis synthesis of L-glufosinate has drawn attention. (bvsalud.org)
Computational1
- These modules are aimed at graduates or experienced practitioners who are interested in continuing their professional development (CPD) in the areas of biocatalysis and computational chemistry. (cardiff.ac.uk)
Therapy1
- In Situ Polymerized Hollow Mesoporous Organosilica Biocatalysis Nanoreactor for Enhancing ROS-Mediated Anticancer Therapy. (nih.gov)
Production2
- Completed Tech transfer of Pyruvate production via biocatalysis. (uiowa.edu)
- In this Perspective, we highlight the significance of redox balance and electron transfer or energy conversion as a foundation to propose a strategy toward high butanol production from biocatalysis to bioelectrocatalysis. (gtiit.edu.cn)
Development1
- This has allowed for the development of several mithramycin analogs ("mithralogs") by combinatorial biosynthesis and/or biocatalysis. (nih.gov)
Search1
- Professor David T. Gibson accepts the 1st Endowed Chair in Biocatalysis in the USA after a worldwide search by a multi-departmental committee. (uiowa.edu)