Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
A family of transmembrane glycoproteins that contain a short cytoplasmic domain, a single-span transmembrane domain, and an extracellular domain with heparin sulfate and CHONDROITIN SULFATE chains. Syndecans interact with a variety of heparin-binding INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS and may play a role in modulating cellular signaling during EMBRYONIC DEVELOPMENT, tumorigenesis, and angiogenesis.
A syndecan that is predominantly expressed during EMBRYONIC DEVELOPMENT. It may play a role in mediating cellular interactions with the EXTRACELLULAR MATRIX and may modulate the signaling activity of certain INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS.
Glycoproteins which have a very high polysaccharide content.
A syndecan that interacts with EXTRACELLULAR MATRIX PROTEINS and plays a role CELL PROLIFERATION and CELL MIGRATION.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
A ubiquitously expressed syndecan that is found in all stages of embryonic development and in most adult tissues. Syndecan-4 is found localized to focal adhesion sites in fibronectin-adherent cells and may play a role the process of CELL MIGRATION and CELL PROLIFERATION.
An enzyme of the isomerase class that catalyzes the eliminative cleavage of polysaccharides containing 1,4-linked D-glucuronate or L-iduronate residues and 1,4-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends. (From Enzyme Nomenclature, 1992) EC
A group of carbon-oxygen lyases. These enzymes catalyze the breakage of a carbon-oxygen bond in polysaccharides leading to an unsaturated product and the elimination of an alcohol. EC 4.2.2.
A syndecan found at high levels in the developing LIMB BUDS. It may play a role in the regulation of MUSCULOSKELETAL DEVELOPMENT by modulating the effects of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS.
A family of GLYCOSYLPHOSPHATIDYLINOSITOL-anchored, cell-surface heparan sulfate proteoglycans that may play a role in CELL GROWTH PROCESSES and CELL DIFFERENTIATION by modulating ligand-receptor interactions.
Inorganic salts of chloric acid that contain the ClO3- ion.
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Enzymes which catalyze the elimination of glucuronate residues from chondroitin A,B, and C or which catalyze the hydrolysis of sulfate groups of the 2-acetamido-2-deoxy-D-galactose 6-sulfate units of chondroitin sulfate. EC 4.2.2.-.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Glycoproteins found on the membrane or surface of cells.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Established cell cultures that have the potential to propagate indefinitely.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Adherence of cells to surfaces or to other cells.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The binding of virus particles to receptors on the host cell surface. For enveloped viruses, the virion ligand is usually a surface glycoprotein as is the cellular receptor. For non-enveloped viruses, the virus CAPSID serves as the ligand.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Inorganic salts of sulfuric acid.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
A naturally occurring glycosaminoglycan found mostly in the skin and in connective tissue. It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (from Merck, 12th ed)
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. The enzyme hydrolyzes triacylglycerols in chylomicrons, very-low-density lipoproteins, low-density lipoproteins, and diacylglycerols. It occurs on capillary endothelial surfaces, especially in mammary, muscle, and adipose tissue. Genetic deficiency of the enzyme causes familial hyperlipoproteinemia Type I. (Dorland, 27th ed) EC
A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It may play an important role in the development of branched CAPILLARIES during EMBRYOGENESIS.
Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
A LDL-receptor related protein involved in clearance of chylomicron remnants and of activated ALPHA-MACROGLOBULINS from plasma.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
A mucopolysaccharide constituent of chondrin. (Grant & Hackh's Chemical Dictionary, 5th ed)
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Proteins prepared by recombinant DNA technology.
The rate dynamics in chemical or physical systems.
Enzymes which catalyze the elimination of delta-4,5-D-glucuronate residues from polysaccharides containing 1,4-beta-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages thereby bringing about depolymerization. EC acts on chondroitin sulfate A and C as well as on dermatan sulfate and slowly on hyaluronate. EC acts on chondroitin sulfate A and C.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
HYALURONAN-containing proteoglycans found in the EXTRACELLULAR MATRIX of a variety of tissues and organs. Several versican isoforms exist due to multiple ALTERNATIVE SPLICING of the versican MESSENGER RNA.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A protein component of the synaptic basal lamina. It has been shown to induce clustering of acetylcholine receptors on the surface of muscle fibers and other synaptic molecules in both synapse regeneration and development.
Nitrous acid (HNO2). A weak acid that exists only in solution. It can form water-soluble nitrites and stable esters. (From Merck Index, 11th ed)
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
The sum of the weight of all the atoms in a molecule.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.
A small leucine-rich proteoglycan that interacts with FIBRILLAR COLLAGENS and modifies the EXTRACELLULAR MATRIX structure of CONNECTIVE TISSUE. Decorin has also been shown to play additional roles in the regulation of cellular responses to GROWTH FACTORS. The protein contains a single glycosaminoglycan chain and is similar in structure to BIGLYCAN.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Specific molecular sites or structures on cell membranes that react with FIBROBLAST GROWTH FACTORS (both the basic and acidic forms), their analogs, or their antagonists to elicit or to inhibit the specific response of the cell to these factors. These receptors frequently possess tyrosine kinase activity.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups. (Enzyme Nomenclature, 1992)
A hyalectin family member that is expressed in neuronal tissue and plays a role in neuronal CELL ADHESION.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
Component of dermatan sulfate. Differs in configuration from glucuronic acid only at the C-5 position.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A 17-kDa single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. It binds to HEPARIN, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 2).
Antibodies produced by a single clone of cells.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Hexameric extracellular matrix glycoprotein transiently expressed in many developing organs and often re-expressed in tumors. It is present in the central and peripheral nervous systems as well as in smooth muscle and tendons. (From Kreis & Vale, Guidebook to the Extracellular Matrix and Adhesion Proteins, 1993, p93)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
A subclass of receptor-like protein tryosine phosphatases that contain an extracellular fibronectin III-like domain along with a carbonic anhydrase-like domain.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Lacritin binds the cell surface heparan sulfate proteoglycan syndecan-1 only in the presence of active heparanase. Heparanase ... is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate ... Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential ... partially or completely cleaves heparan sulfate to expose a binding site in the N-terminal 50 amino acids of syndecan-1. The ...
... cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1). Binding utilizes an ... cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein. A G-protein-coupled ... Yet, long heparan sulfate chains interfere with lacritin binding. Since syndecans are always decorated with heparan sulfate, ... or lymphoblastic cells. Cell surface proteoglycan syndecan-1 is partly responsible. Biotinylated cell surface proteins from a ...
"Differential expression of cell surface heparan sulfate proteoglycans in human mammary epithelial cells and lung fibroblasts". ... with binding largely via heparan sulfate chains. The syndecans mediate cell binding, cell signaling, and cytoskeletal ... heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecan-1 protein functions as an ... cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein. Three SDC1 elements ...
... heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell ... Mahley RW, Ji ZS (1999). "Remnant lipoprotein metabolism: key pathways involving cell-surface heparan sulfate proteoglycans and ... and 90-kilodalton core proteins of cell surface-associated heparan sulfate proteoglycans of lung fibroblasts. Prediction of an ... exits from cells via a leaderless secretory pathway and binds to extracellular matrix-associated heparan sulfate proteoglycans ...
... exits from cells via a leaderless secretory pathway and binds to extracellular matrix-associated heparan sulfate proteoglycans ... Syndecans are normally found on the cell surface of fibroblasts and epithelial cells. Syndecans interact with fibronectin on ... "Entrez Gene: SDC4 syndecan 4". Woods A, Couchman JR (February 1994). "Syndecan 4 heparan sulfate proteoglycan is a selectively ... "Syndecan 4 heparan sulfate proteoglycan is a selectively enriched and widespread focal adhesion component". Mol Biol Cell. 5 (2 ...
1992). "Biology of syndecans: a family of transmembrane heparan sulfate proteoglycans". Annu. Rev. Cell Biol. 8: 365-393. doi: ... Syndecans are expressed on the cell surface in a cell-specific manner. For example, in mouse cells and tissues, syndecan 1 is ... Syndecans bind to structural extracellular matrix molecules such as collagens I, III, V, fibronectin, thrombospondin, and ... Klaus Elenius & Markku Jalkanen (1994). "Function of the syndecans - a family of cell surface proteoglycans". Journal of Cell ...
... exits from cells via a leaderless secretory pathway and binds to extracellular matrix-associated heparan sulfate proteoglycans ... 1994). "Isolation of a neuronal cell surface receptor of heparin binding growth-associated molecule (HB-GAM). Identification as ... "Regulated expression and subcellular localization of syndecan heparan sulfate proteoglycans and the syndecan-binding protein ... "Molecular cloning and characterization of N-syndecan, a novel transmembrane heparan sulfate proteoglycan". J Cell Biol. 117 (1 ...
... smooth muscle cell adhesion and stimulates chemotaxis through integrin α6β1 and cell surface heparan sulfate proteoglycans". ... Where examined, syndecan-4 has been identified as the HSPG critical for CCN1 functions. The CYR61 binding sites for some of ... "Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin α6β1 and cell surface heparan sulfate proteoglycans". ... and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic ...
"Functions of cell surface heparan sulfate proteoglycans". Annual Review of Biochemistry. 68: 729-77. doi:10.1146/annurev. ... This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules. Heparan sulfate ... Given several HSPGs (perlecan, syndecan 1/3, glypican) are upregulated and growth factor signaling through FGF-2, Wnt, BMP, and ... "QSulf1 remodels the 6-O sulfation states of cell surface heparan sulfate proteoglycans to promote Wnt signaling". The Journal ...
... syndecan, or glypican. Sulfation of heparan sulfate GAGs helps give diversity to cell surface proteins and provides them with a ... in mast cells the AT-III-binding pentasaccharaide is synthesized with essential heparan sulfate sulfation steps. The binding of ... Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many ... In contrast, heparan sulfate complexes have been shown to bind to HIV-1 and prevent it from entering the cell through its ...
"Regulated expression and subcellular localization of syndecan heparan sulfate proteoglycans and the syndecan-binding protein ... It interacts with the transcription factor TBR1 and binds to several cell-surface proteins including neurexins and syndecans. ... and syndecan transmembrane heparan sulfate proteoglycans". J. Neurosci. 21 (11): 3764-70. doi:10.1523/JNEUROSCI.21-11- ... "Direct interaction of CASK/LIN-2 and syndecan heparan sulfate proteoglycan and their overlapping distribution in neuronal ...
Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. Binding to CD4 ... gp120 can also easily be shed from the surface of the virus and captured by T cells due to its loose binding with gp41. A ... "Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1". Proc. Natl. Acad. Sci. U.S.A. 104 (49): 19464-9. doi: ... Consequently, anything which binds to gp120 or its targets can physically block gp120 from binding to a cell. Only one such ...
One study has suggested that, by blocking TSP1 from binding to its cell surface receptor (CD47) normal tissue confers high ... syndecan, and integrin-associated protein (IAP or CD47). It also interacts with numerous proteases involved in angiogenesis, ... This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to ... Bound protein fragments of the type I repeats have been shown to serve as attachment factors for both ECs and melanoma cells. ...
... also binds to other extracellular matrix proteins such as collagen, fibrin, and heparan sulfate proteoglycans (e.g ... These soluble dimers bind to α5β1 integrin receptors on the cell surface and aid in clustering the integrins. The local ... heparin-binding and syndecan-binding (III12-14). Fibronectin has numerous functions that ensure the normal functioning of ... "cell-binding domain" of fibronectin. The RGD sequence (Arg-Gly-Asp) is located in III10 and is the site of cell attachment via ...
... ProteoGlycan) in which two or three HS chains are attached in close proximity to cell surface or extracellular ... The antibody binds heparan sulfate, not chondroitin sulfate. The binding of HS20 to heparan sulfate requires sulfation at both ... The major cell membrane HSPGs are the transmembrane syndecans and the glycosylphosphatidylinositol (GPI) anchored glypicans. ... "heparan sulfate interactome" is more correct. The functions of heparan sulfate binding proteins ranges from extracellular ...
... -2 (GPC2) is a cell surface heparan sulfate proteoglycan that is important for neuronal cell adhesion and neurite ... the heparan sulfate chains attach to the protein core. Therefore, unlike syndecans the heparan sulfate glycosaminoglycan chains ... and that the 3-O-sulfation in GlcNS6S3S enhances the binding of Wnt to the heparan sulfate glypican. A cysteine-rich domain at ... Glypicans constitute one of the two major families of heparan sulfate proteoglycans, with the other major family being ...
Steigemann P, Molitor A, Fellert S, Jäckle H, Vorbrüggen G (February 2004). "Heparan sulfate proteoglycan syndecan promotes ... Binding of Slit to Robo induces binding of SrGAP1 to the CC3 domain of Robo1, which leads to downstream deactivation of Cdc42, ... Slits mediate cell communication in many diverse systems, regulating the guidance, cell migration and polarization of many ... preventing it from accessing the surface of the growth cone. Thus, when Comm is expressed, axons are unaffected by the presence ...
This releases growth factors bound to the heparan sulfate, specifically FGF-10. Addition of heparanase to cell culture of ... proteoglycan that binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is ... heparan sulfate proteoglycan expression has been verified, although no distinction between syndecan and perlecan has been made ... also known as basement membrane-specific heparan sulfate proteoglycan core protein (HSPG) or heparan sulfate proteoglycan 2 ( ...
Lacritin binds the cell surface heparan sulfate proteoglycan syndecan-1 only in the presence of active heparanase. Heparanase ... is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate ... Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential ... partially or completely cleaves heparan sulfate to expose a binding site in the N-terminal 50 amino acids of syndecan-1. The ...
Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1). Binding utilizes an ... cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1s core protein. A G-protein-coupled ... Yet, long heparan sulfate chains interfere with lacritin binding. Since syndecans are always decorated with heparan sulfate, ... or lymphoblastic cells. Cell surface proteoglycan syndecan-1 is partly responsible. Biotinylated cell surface proteins from a ...
The syndecan family has four members, of which syndecan-1 is the most studied and best characterized. We have studied the ... The syndecans are a family of cell-surface heparan sulphate proteoglycans that regulate cell behaviour by binding extracellular ... The syndecans are a family of cell-surface heparan sulphate proteoglycans that regulate cell behaviour by binding extracellular ... The syndecan family has four members, of which syndecan-1 is the most studied and best characterized. We have studied the ...
Recent Insights into Cell Surface Heparan Sulphate Proteoglycans and Cancer. Research output: Contribution to journal › Review ... Characterisation of stromal-cellular mechanotransduction through syndecan-4. Research output: Contribution to conference › ... Differences in human skin between the epidermal growth factor receptor distribution detected by EGF binding and monoclonal ... Heparan sulfate proteoglycans of rat embryo fibroblasts. A hydrophobic form may link cytoskeleton and matrix components.. Woods ...
... factors that reduce progressive organ fibrosis Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for ... rabbit aneurysms Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells Prioritized ... Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway Multiyear follow-up of AAV5-hFVIII-SQ gene ... cell line from human pluripotent stem cells GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell ...
... factors that reduce progressive organ fibrosis Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for ... rabbit aneurysms Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells Prioritized ... Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway Multiyear follow-up of AAV5-hFVIII-SQ gene ... cell line from human pluripotent stem cells GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell ...
... factors that reduce progressive organ fibrosis Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for ... rabbit aneurysms Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells Prioritized ... Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway Multiyear follow-up of AAV5-hFVIII-SQ gene ... cell line from human pluripotent stem cells GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell ...
  • 7. The proteoglycan of claim 3, further comprising at least one chondroitin sulfate glycosaminoglycan. (google.es)
  • Chondroitin sulfate chains on syndecan-1 and syndecan-4 from normal murine mammary gland epithelial cells are structurally and functionally distinct and cooperate with heparan sulfate chains to bind growth factors. (openrepository.com)
  • Structural characterization of heparan sulfate and chondroitin sulfate of syndecan-1 purified from normal murine mammary gland epithelial cells. (openrepository.com)
  • Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. (uniprot.org)
  • Proteoglycans (PGs) are distributed ubiquitously as a component of extracellular matrix (ECM) at the cell surface and bear glycosaminoglycans (GAGs) such as heparan sulfate (HS) and chondroitin sulfate (CS). (rupress.org)
  • The mammalian syndecan family comprises four members, each with large heparan sulfate and chondroitin sulfate chains covalently attached to the extracellular domain ( 3 ) and short cytoplasmic tails that interact with a number of signaling adaptors and enzymes ( 1 ). (sciencemag.org)
  • 500 kD) and contain both heparan and chondroitin sulfate moieties. (biomedcentral.com)
  • Syndecans are composed of sulfated glycosaminoglycans (GAGs), heparan sulfate (HS) or both HS and chondroitin sulfate (CS), attached covalently to core proteins. (readbyqxmd.com)
  • Syndecan and glypican are membrane-bound proteoglycans which carry chondroitin sulfate and heparan sulfate side chains. (biomedcentral.com)
  • Proteoglycans consist of a core protein and an associated glycosaminoglycan (GAG) chain of heparan sulfate, chondroitin sulfate, dermatan sulfate or keratan sulfate, which are attached to a serine residue. (openrepository.com)
  • Analyzing tumor samples with different degrees of histological differentiation we determined the existence of important alterations in chondroitin sulfate (CS) chains. (frontiersin.org)
  • The other GAGs are chondroitin sulfate (CS), dermatan sulfate (DS), keratan sulfate (KS), and heparan sulfate (HS). (sigmaaldrich.com)
  • Chondroitin and heparan sulfate are extensively modified by sulfation 1,2,3 . (sigmaaldrich.com)
  • When plated onto a chondroitin sulfate proteoglycan (CSPG) surface, Hb axons fasciculated into bundles, whereas this was not observed when the neurons were plated on bovine serum albumin. (sciencemag.org)
  • The syndecan family of transmembrane proteins comprises a major class of cell surface heparan sulfate proteoglycans (HSPGs). (jneurosci.org)
  • On the basis of their ability to mediate dynamic cell-matrix interactions and to bind to diverse polypeptide signaling factors, syndecan HSPGs could play a supporting role in many aspects of neural development, including cell migration, neurite extension, and synapse assembly and plasticity. (jneurosci.org)
  • In this report, we show that one of the HSPGs, syndecan-4, specifically binds to the HBD of OPN via HS moiety. (rupress.org)
  • Heparan sulfate proteoglycans (HSPGs) are constituents of the extracellular matrix, and through their heparan sulfate chains and protein core, modulate multiple events that occur during the metastatic cascade. (frontiersin.org)
  • Here we show that cell-surface heparan sulphate proteoglycans (HSPGs) support integrin α2β1-mediated adhesion to collagen. (vtt.fi)
  • Cells made devoid of HSPGs either by genetic means or by enzymatic digestions were unable to adhere to collagen via α2β1 integrin. (vtt.fi)
  • Heparan sulfate proteoglycans (HSPGs) 1 are important regulators of cell adhesion and cell signaling. (go.jp)
  • There is mounting evidence that HSPGs are remodeled by enzymes present on the surface of tumor cells or within the extracellular compartment. (go.jp)
  • This remodeling within the tumor microenvironment leads to structural and functional alterations in HSPGs that regulate the behavior of tumor cells, including their growth and metastatic properties. (go.jp)
  • Syndecans (Syns) are transmembrane heparan sulfate proteoglycans (HSPGs) and comprise a family of four members, Syn 1-4. (uio.no)
  • Syndecans comprise a major family of cell surface heparan sulfate proteoglycans (HSPGs). (readbyqxmd.com)
  • Furthermore, mice ablated for the syndecan-1 or -4 gene do not show major developmental abnormalities, but they do show striking pathological phenotypes when challenged with infectious or inflammatory stimuli and conditions, suggesting that certain functions of syndecans are specific and cannot be compensated for by other syndecans or other HSPGs. (readbyqxmd.com)
  • The encoded proteins of these genes belong to an EXT family of proteins that have or are closely related to glycosyltransferase activities required for biosynthesis of heparan sulfate proteoglycans (HSPGs). (biologists.org)
  • Moreover, all three morphogens were accumulated in the front of EXT mutant cells, suggesting that these morphogens require HSPGs to move efficiently. (biologists.org)
  • Recently, several reports suggest that heparan sulfate proteoglycans (HSPGs) play a key role in morphogen transport and/or signaling (reviewed by Perrimon and Bernfield, 2000 ). (biologists.org)
  • HSPGs are abundant cell surface molecules and are part of the extracellular matrix. (biologists.org)
  • HSPGs consist of a protein core (such as syndecans and glypicans) to which heparan sulfate glycosaminoglycan (HS GAG) chains are attached. (biologists.org)
  • Heparan sulfate proteoglycans (HSPGs) modulate the binding and activation of signaling pathways of specific growth factors, such as fibroblast growth factor-2 (FGF-2). (biomedcentral.com)
  • The action of these potent growth mediators is known to be regulated by adsorption to heparan sulfate proteoglycans (HSPGs) on the surface and within the extracellular matrix of other neighboring cells, which respectively promote or restrict interactions with their signal-transducing receptors on target cells. (ox.ac.uk)
  • Here we report on the nature of HSPGs inducibly expressed on the surface of macrophages that confer these cells with the capacity to regulate endogenous growth factor activity. (ox.ac.uk)
  • The sulfation pattern of HS influences signaling events mediated by heparan sulfate proteoglycans (HSPGs) located on cell surface, which are critical for the interactions with growth factors and their receptors. (biomedcentral.com)
  • Among its many roles, membrane HSPGs can bind to cytokines, chemokines and growth factors, protecting them from proteolysis. (biomedcentral.com)
  • The attractive effect of Sema5A was lost if heparan sulfate proteoglycans (HSPGs) were diminished on the Hb neurons by either heparinase treatment or inhibition of HSPG biosynthesis. (sciencemag.org)
  • Syndecan-1 is a cell-surface, heparan-sulphate proteoglycan (HSPG) predominantly expressed by epithelial cells. (nih.gov)
  • In addition, we show that soluble syndecan-1 ectodomain purified from mouse mammary epithelial cells stimulates the activity of a Wnt-1 homologue in a tissue culture assay. (nih.gov)
  • Lacritin also promotes tear secretion, the proliferation and survival of epithelial cells, and corneal wound healing Lacritin is thus a multifunctional prosecretory mitogen with cell survival activity. (wikipedia.org)
  • Some lacritin is produced by the meibomian gland, and by epithelial cells of the conjunctiva and cornea. (wikipedia.org)
  • Lacritin targets a restricted group of epithelial cells (including human corneal epithelia), and not fibroblastic, glioma, or lymphoblastic cells. (wikipedia.org)
  • Syndecan-1 (SDC1) is 1 of 4 cell surface heparan sulfate proteoglycans that are predominantly expressed by epithelial cells and plasma cells in adult tissues. (medsci.org)
  • Cultured alveolar epithelial cells from septic rats mimic in vivo septic lung. (biomedsearch.com)
  • Therefore we hypothesized that alveolar epithelial cells isolated from septic animals develop tight junctions with different protein composition and reduced barrier function relative to alveolar epithelial cells from healthy animals. (biomedsearch.com)
  • Syndecan-1 and -4 synthesized simultaneously by mouse mammary gland epithelial cells bear heparan sulfate chains that are apparently structurally indistinguishable. (openrepository.com)
  • Syndecans are the major cell-surface PGs expressed by virtually all epithelial cells. (rupress.org)
  • Syndecan-1 is predominantly present on epithelial cells, whereas syndecan-4 is found ubiquitously but most notably on fibroblasts ( 3 ), and these two family members exhibit the closest functional similarities. (sciencemag.org)
  • Hayashida K, Johnston DR, Goldberger O, Park PW: Syndecan-1 expression in epithelial cells is induced by transforming growth factor beta through a PKA-dependent pathway. (exbio.cz)
  • HSULF-1 mRNA expression was assessed in five normal cells (primary human lung alveolar type 2 (hAT2) cells, adult lung fibroblasts (16Lu), fetal lung fibroblasts (HFL), human bronchial epithelial cells (HBE), and primary human lung fibroblasts (HLF)) and five lung cancer cell lines (A549, H292, H1975, H661, and H1703) using quantitative real time polymerase chain reaction (qRT-PCR). (biomedcentral.com)
  • EC is a constituent of normal tears 2 , 3 and is expressed by corneal epithelial cells 4 (NEIBank) where it is UVB inducible. (arvojournals.org)
  • Hepatocyte growth factor is a heparin-binding cytokine that influences the behavior of both endothelial and epithelial cells. (pubmedcentralcanada.ca)
  • Immunohistochemistry, lectin-based histochemistry, conventional staining techniques, and proteomic assays were performed on eyes and cultured corneal epithelial cells from wild-type and Pax6 +/ − littermates. (arvojournals.org)
  • Lectin cytochemistry of corneal epithelial cells suggested a partial failure of glycoprotein trafficking. (arvojournals.org)
  • 6 7 8 9 In vitro, Pax6 +/ − corneal epithelial cells do not respond to wounding within the first 1 to 2 hours, in contrast to Pax6 +/+ cells that start to migrate into the wound within a few minutes. (arvojournals.org)
  • syndecan - An integral membrane proteoglycan (250 300 kD) associated largely with epithelial cells. (enacademic.com)
  • Initially, nonmalignant human mammary epithelial cells (MCF-10A) were cultured within a reconstituted basement membrane (BM) where they formed three-dimensional (3-D) polarized, growth-attenuated, multicellular acini, enveloped by a continuous endogenous BM. (genes2cognition.org)
  • 14 ] reported that in prostate tumors, complex changes occur in PGs, with decreased expression of decorin and lumican, an overall increase in syndecan-1 and glypican-1 in tumor stroma, along with the disappearance of agrecan in tumor epithelial cells. (biomedcentral.com)
  • Reduced Sip1 binding to Merlin also produces defects in adhesion in follicle epithelial cells. (sdbonline.org)
  • It has been shown that receptor-binding of VEGF and bFGF in endothelial cells is dependent on surface HSPG or heparan sulfate but transmembrane heparan sulfate proteoglycans (syndecans or CD44v3) have not been identified in human endothelial cells. (aacrjournals.org)
  • One important feature of HSPG is the ability to interact with a variety of growth factors, cytokines, chemokines, and ECM proteins, thereby regulating wound healing, tissue remodeling, hemostasis, and inflammation ( 1 ). (rupress.org)
  • Syndecans C Main Alisertib inhibitor database Hubs for Swelling, Lipid Satiety and Rate of metabolism Control In mammalian cells, the Syndecan (SDC) family members contain four type I transmembrane NAK-1 HSPG (SDC1-4) (Desk 1). (lecollege.org)
  • HSPG-deficient cells also displayed impaired spreading and actin organization on collagen. (vtt.fi)
  • Among different HSPG molecules syndecan-1 was found to play an important role in supporting α2β1 integrin-mediated adhesion. (vtt.fi)
  • these include very low density lipoprotein receptor (VLDL-R), low density lipoprotein receptor-related protein (LRP), and heparan sulfate proteoglycans (HSPG). (biomedcentral.com)
  • Using ligand binding antagonists, we observed that HSPG, rather than VLDL-R or LRP, play a primary role in the uptake of DiI-lableled apoE-VLDL by mature adipocytes. (biomedcentral.com)
  • These results suggest that cell surface HSPG is required for fatty acid transport across the plasma membrane of adipocytes. (biomedcentral.com)
  • In the first model, LPL originating in adipose or muscle tissue is transported across the endothelial barrier where it remains tethered to the lumenal surface through its affinity for heparan sulfate proteoglycans (HSPG). (biomedcentral.com)
  • We reveal that activated human macrophages express only a single major 48-kDa cell surface HSPG, syndecan-2 (fibroglycan) as the result of de novo RNA and protein synthesis. (ox.ac.uk)
  • In addition, we demonstrate this macrophage HSPG selectively binds the macrophage-derived growth factors FGF-2, vascular endothelial growth factor and heparin binding EGF and can present FGF-2 in a form that transactivates receptor-bearing BaF32 cells. (ox.ac.uk)
  • Furthermore, disagreeing reviews of whether PHSRN only is definitely able of assisting cell adhesion possess been a subject of argument over the previous 10 years.15?18 Although 51 and V3 integrins serve as the primary cell surface area TAK-960 receptors that mediate adhesion, syndecan-4, a transmembrane heparan sulfate proteoglycan (HSPG), is a coreceptor for FN.20,21 A heparan sulfate (HS) binding website covers FNIII12-14. (immune-source.com)
  • Syndecan-3 is a transmembrane heparan sulfate proteoglycan (HSPG) expressed in SCs that interacts with extracellular matrix proteins and growth factors through its ectodomain, and with cytoskeletal proteins and intracellular signaling molecules through its intracellular domain. (rupress.org)
  • Sema5A coprecipitated with the HSPG syndecan-3, suggesting a direct interaction. (sciencemag.org)
  • Keratinocyte migration during epidermal repair depends on interactions between cellular heparan sulfate proteoglycan receptors, syndecan-1 and -4, and the C-terminal globular domains (LG45) of the extracellular matrix protein laminin 332. (cf.ac.uk)
  • This study investigates the molecular basis of the binding specificity of the syndecan-1 and -4 receptors expressed by human keratinocytes. (cf.ac.uk)
  • While most of these proteins are neurotransmitters, receptors, or intracellular signaling molecules, recent data indicate an important role for a unique family of proteins called syndecans. (jci.org)
  • Expression of c-met significantly correlated with expression of endothelin A and B receptors, vascular endothelial growth factor (VEGF)-A and fibroblast growth factor receptor-1, whereas E-cadherin expression correlated significantly with endothelin A receptor, VEGF-A and VEGF-C staining. (biomedcentral.com)
  • the melanocortin system consists of 1 ) the melanocortin peptides α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and adrenocorticotropic hormone (ACTH), 2 ) a family of five seven-transmembrane G protein-coupled melanocortin receptors, and 3 ) the endogenous melanocortin antagonists agouti and agouti-related protein (AGRP). (physiology.org)
  • The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. (mybiosource.com)
  • The syndecan transmembrane proteoglycans synergize with receptors for extracellular matrix molecules and growth factors to initiate cytoplasmic signals in response to a range of extracellular stimuli. (sciencemag.org)
  • The work describes a mechanistic investigation into inside-to-outside syndecan signaling and highlights several gaps in our understanding of the relation between cell-surface receptors and proteases. (sciencemag.org)
  • Proteoglycans are not the primary receptors of extracellular matrix molecules, growth factors, or chemokines, but they cooperate with the prototypic receptors through simultaneous ligand engagement. (sciencemag.org)
  • The consequences of ectodomain competition are twofold, as both the signaling capabilities of the intact syndecan and the associated prototypic receptors are compromised ( 7 , 8 ) ( Fig. 1 ). (sciencemag.org)
  • In vitro studies indicate that many extracellular molecules and cell surface receptors interact with the HS chains and core protein of perlecan. (go.jp)
  • Syns have important roles as cell surface receptors involved in cell-cell and/or cell-matrix interactions. (uio.no)
  • Additional information: Clone REA640 displays negligible binding to Fc receptors. (miltenyibiotec.com)
  • Extracellular fatty acids that are available for adipocyte uptake are either 1) associated with circulating albumin, 2) hydrolyzed from triacylglycerol-rich lipoprotein particles by lipoprotein lipase, or 3) in the form of VLDL particles which can be directly internalized by adipocyte lipoprotein receptors. (biomedcentral.com)
  • Although DENVs reportedly attach to a variety of receptors on these cells, consensus DENV receptors have not been defined. (hindawi.com)
  • In this review, we summarize receptors for DENVs on different cells identified in recent years. (hindawi.com)
  • It is known that E protein is not only a functional protein molecule that binds to receptors on the host cell membrane but also a major antigen, which can induce neutralizing antibody and host specific protective immunity [ 1 - 3 ]. (hindawi.com)
  • Recently there have been significant advances in understanding interactions of DENV with target cells such as dendritic cells (DCs), macrophages, vascular endothelial cells (VEC), and hepatocytes, and we would summarize receptors for DENVs on different cells identified in last several years in this review (Table 1 ). (hindawi.com)
  • Fibroblast growth factor 2 (FGF2) induces endothelial cell migration and angiogenesis through two classes of receptors: receptor tyrosine kinases, such as FGF receptor 1 (FGFR1), and heparan sulfate proteoglycans, such as syndecan 4 (S4). (antibodyassay.com)
  • As with other syndecans, S4 bears heparan sulfate chains on its extracellular domain name that can hole numerous heparan-binding growth factors and other families of transmembrane growth factor receptors, such as integrins (2, 3). (antibodyassay.com)
  • Although cell surface receptors may initiate signaling cascades from the membrane, numerous signaling events require cytoplasmic localization, and the process of endocytosis can exert fine spatiotemporal control over signaling (18). (antibodyassay.com)
  • Casitas b-lineage lymphoma (c-Cbl) is an E3 ubiquitin ligase that has an important role in regulating the degradation of cell surface receptors. (jci.org)
  • Syndecans: from peripheral co-receptors to mainstream regulators of cell behaviour. (springer.com)
  • C. elegans has numerous putative cell adhesion receptors, many of which have vertebrate homologues. (biologists.org)
  • for information on other cell surface receptors please refer to Hutter et al. (biologists.org)
  • Syndecans are anchored to the plasma membrane via a single-span transmembrane protein and may therefore act as receptors. (biomedcentral.com)
  • Uptake of human papillomavirus virus-like particles by dendritic cells is mediated by Fc γ receptors and contributes to acquisition of T cell immunity. (microbiologyresearch.org)
  • Here we focus on the role of cellular adhesion receptors in the metastatic cascade and discuss evidence for heparin as an inhibitor of cell adhesion. (hindawi.com)
  • 2005). In addition, it plays a role in cell adhesion through integrin receptors and has been shown to influence smooth muscle cell proliferation (Yanigasawa et al. (wikipathways.org)
  • Formation of fibrils appears to require cell surface structures suggesting an involvement of cell surface receptors. (wikipathways.org)
  • A summary of the proteins in the basement membrane and their characterized cell surface receptors are given in Table 1 6 . (sigmaaldrich.com)
  • Compositions containing papillomavirus VLPs or capsomeres are used, alone or in combination with other agents, as microbicides that substantially block papillomavirus binding receptors on the surface or vicinity of cells in a tissue to be treated with the composition. (patentsencyclopedia.com)
  • The invention can be used to inhibit papillomavirus infection or infection by another virus that utilizes the same binding receptors during the infection process. (patentsencyclopedia.com)
  • and contacting a tissue of an individual with the composition under conditions effective to substantially block papillomavirus binding receptors on the surface or vicinity of cells in the tissue, wherein said contacting is effective to inhibit virus infection of the individual. (patentsencyclopedia.com)
  • We have defined the molecular basis of cell adhesion to fibrillin-1, the major structural component of extracellular microfibrils that are associated with elastic fibres. (biologists.org)
  • Using human dermal fibroblasts, and recombinant domain swap fragments containing the Arg-Gly-Asp motif, we have demonstrated a requirement for upstream domains for integrin-α 5 β 1 -mediated cell adhesion and migration. (biologists.org)
  • An adjacent heparin-binding site, which supports focal adhesion formation, was mapped to the fibrillin-1 TB5 motif. (biologists.org)
  • Site-directed mutagenesis revealed two arginine residues that are crucial for heparin binding, and confirmed their role in focal adhesion formation. (biologists.org)
  • These integrin and syndecan adhesion motifs juxtaposed on fibrillin-1 are evolutionarily conserved and reminiscent of similar functional elements on fibronectin, highlighting their crucial functional importance. (biologists.org)
  • It has a single Arg-Gly-Asp (RGD) cell adhesion motif within the fourth TB repeat (TB4), which is surrounded by polar, charged amino acids and likely to be solvent exposed. (biologists.org)
  • Previous studies demonstrate that cell adhesion to fibrillin-1 and fibrillin-2 involves the RGD motif on TB4. (biologists.org)
  • In this study, we have defined a central region of fibrillin-1 in which cell adhesion, spreading and focal adhesion formation are profoundly modulated by specific domains upstream and downstream of the RGD motif. (biologists.org)
  • The presence of functionally similar cell adhesion elements on fibrillin-1 and the structurally unrelated adhesive glycoprotein, fibronectin, highlight their critical evolutionary importance in cell-matrix biology. (biologists.org)
  • They have many biological roles including regulating cell growth, differentiation, and adhesion. (rndsystems.com)
  • Tenascin-C (TN-C) is unique for its cell adhesion modulatory function. (sigmaaldrich.com)
  • We have shown that TNIIIA2, a synthetic 22-mer peptide derived from TN-C, stimulated beta1 integrin-mediated cell adhesion of nonadherent and adherent cell types, by inducing activation of beta1 integrin. (sigmaaldrich.com)
  • The syndecan-CASK interaction may be involved in intercellular signaling and/or cell adhesion. (jneurosci.org)
  • UspA1 binds a range of extracellular proteins including fibronectin, and the epithelial cellular receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). (exeter.ac.uk)
  • Through these domains, syndecan-1 is proposed to have roles in growth factor action, extracellular matrix adhesion, and cytoskeletal organization that controls cell morphology. (rupress.org)
  • To study the role of syndecan-1 in cell adhesion and cytoskeleton reorganization, mouse syndecan-1 cDNA was transfected into human Raji cells, a lymphoblastoid cell line that grows as suspended cells and exhibits little or no endogenous cell surface heparan sulfate. (rupress.org)
  • Previous studies have demonstrated that the colony opacity-associated (Opa) proteins on the N. gonorrhoeae surface can bind human carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) on CD4 + T cells to suppress T cell activation and proliferation. (pubmedcentralcanada.ca)
  • Heparan sulphate proteoglycan syndecan-1 modulates cell proliferation, adhesion, migration and angiogenesis. (biomedcentral.com)
  • Expression of adhesion molecules, VAP-1 and CD44v3, in intra- and peritumoral microvessels of human skin melanoma. (aacrjournals.org)
  • Here we have analysed the expression of an adhesion molecule, VAP-1, in melanoma associated blood vessels in 28 primary skin melanoma cases using immunocytochemistry and immunoelectron microscopy. (aacrjournals.org)
  • These results support the idea that the phenotype of tumoral blood vessels is different from normal vessels: expression of certain adhesion molecules is frequently missing (VAP-1), while others are preserved (CD44v3) which may have a role in tumor progression. (aacrjournals.org)
  • In endothelial cells, inactivation of inhibits granulocyte adhesion and diminishes binding of L-selectin (47) and leads to decreased leukocyte recruitment in DKD (48). (lecollege.org)
  • Leukocyte adhesion to the endothelium and following extravasation during inflammation is mediated by cell surface molecules like selectins, cell adhesion molecules, integrins and chemokines. (dog.org)
  • Syndecan-1-knockout mice show an increased adhesion of leukocytes to the endothelium. (dog.org)
  • Several different receptor molecules act in concert to regulate cell adhesion. (vtt.fi)
  • However, very little is known about possible functional synergism between proteoglycans and integrins during adhesion of cells to collagen, although collagen is the most abundant protein in the human body. (vtt.fi)
  • Our findings thus suggest that a previously unknown link between integrin α2β1 and syndecan-1 is important in regulating cell adhesion to collagen and in triggering integrin downstream signalling. (vtt.fi)
  • Small-molecule-induced clustering of heparan sulfate promotes cell adhesion. (nih.gov)
  • A dumbbell-shaped small molecule that promotes cell adhesion and growth. (nih.gov)
  • Syndecans-1 and -4 mediate intercellular adhesion following their transfection into human B lymphoid cells. (nih.gov)
  • Structural requirements of heparan sulfate for the binding to the tumor-derived adhesion factor/angiomodulin that induces cord-like structures to ECV-304 human carcinoma cells. (nih.gov)
  • Syndecan-1 (CD138) is a heparan sulfate-bearing proteoglycan present on the surface of myeloma cells where it mediates myeloma cell-cell and cell-extracellular matrix adhesion. (uab.edu)
  • Moreover, the uropod is the cell pole preferentially involved in aggregation of myeloma cells and in adhesion of myeloma cells to osteoblast-like cells. (uab.edu)
  • These data indicate that syndecan-1 is targeted to the uropod of polarized myeloma cells and that this targeting plays a role in promoting cell-cell adhesion and may also regulate the biological activity of heparinbinding cytokines. (uab.edu)
  • Within this functional domain, syndecan-1 promotes cell-cell adhesion and concentration of heparin binding growth factors. (uab.edu)
  • The glycosaminoglycan-binding domain of decoy receptor 3 is essential for induction of monocyte adhesion. (ox.ac.uk)
  • DcR3.Fc has been shown to stimulate cell adhesion and to modulate cell activation and differentiation by triggering multiple signaling cascades that are independent of its three known ligands. (ox.ac.uk)
  • In this study we found that DcR3.Fc-induced cell adhesion was inhibited by heparin and heparan sulfate, and that DcR3.Fc was unable to bind Chinese hamster ovary K1 mutants defective in glycosaminoglycan (GAG) synthesis. (ox.ac.uk)
  • Moreover, a fusion protein comprising the GAG-binding region of DcR3 with an Fc fragment (DcR3_HBD.Fc) has the same effect as DcR3.Fc in activating protein kinase C and inducing cell adhesion. (ox.ac.uk)
  • Compared with wild-type THP-1 cells, cell adhesion induced by DcR3.Fc was significantly reduced in both CD44v3 and syndecan-2 knockdown THP-1 cells. (ox.ac.uk)
  • Therefore, we propose a model in which DcR3.Fc may bind to and cross-link proteoglycans to induce monocyte adhesion. (ox.ac.uk)
  • Nevertheless, syndecan-1 on MPC-11 mediates cell adhesion to type I collagen, whereas P3 cells do not bind collagen. (semanticscholar.org)
  • INTRODUCTION Syndecan 4 (S4) is usually a transmembrane proteoglycan involved in the rules of numerous cellular processes, including cell adhesion and migration (1, 2). (antibodyassay.com)
  • It soon became apparent that they could support cell adhesion, and now it is known that. (springer.com)
  • IgCAMs are single-span transmembrane proteins that mediate cell-cell adhesion via homophilic or heterophilic interactions. (biologists.org)
  • F02G3.1 and SSSD1.1 have extracellular domains similar to the vertebrate neural cell adhesion molecule NCAM. (biologists.org)
  • 2002). Two GPI-anchored IgCAMs, RIG-3 and WRK-1, have sequence similarity, respectively, to Drosophila Klingon and Wrapper, which have been proposed to mediate cell adhesion (reviewed in Teichmann and Chothia, 2000 ). (biologists.org)
  • Cell adhesion molecules play a significant role in cancer progression and metastasis. (hindawi.com)
  • In addition, direct tumor cell interactions with platelets, leukocytes, and soluble components significantly contribute to cancer cell adhesion, extravasation, and the establishment of metastatic lesions. (hindawi.com)
  • Tumor cells in circulation interact also with platelets and leukocytes that further contribute to tumor cell adhesion, extravasation, and the establishment of metastatic lesions. (hindawi.com)
  • While cell-cell interactions facilitating tumor cell adhesion in the vasculature of specific organs are essential steps in the metastatic cascade, inhibition of these interactions represent a therapeutically useful target for attenuation of metastasis (Figure 1 ). (hindawi.com)
  • Two major cell adhesion molecule families, selectins and integrins, have been identified to participate in metastasis [ 3 - 6 ]. (hindawi.com)
  • 12 In vivo studies have suggested that changes in the adhesive properties of mutant cells may underlie many of the observed morphologic abnormalities, and Pax6 controls expression of cell adhesion molecules, most of which are glycoproteins. (arvojournals.org)
  • In this study, the ability of fibulin-1 to modulate adhesion, spreading and motility-promoting activities of FN was investigated. (biologists.com)
  • linker.19 Spatial orientation and ranking of these signals are crucial for inducing synergistic effects on cell adhesion and migration. (immune-source.com)
  • nevertheless, equivalent to the synergistic impact of PHSRN and RGD on cell adhesion, these little sequences or molecules are much less effective in promoting cell attachment alone. (immune-source.com)
  • Dedicated adhesion molecules on myelinating cells and axons govern node organization. (rupress.org)
  • The tumor suppressor protein Merlin is thought to regulate cell proliferation and cell adhesion through interaction with protein partners. (sdbonline.org)
  • This study investigated how the Drosophila NHERF1 orthologue, Sip1 , and Merlin function to regulate cell proliferation and adhesion. (sdbonline.org)
  • 3. A soluble proteoglycan recombinantly produced by a transformed eukaryotic cell capable of producing heparan sulfate glycosaminoglycans, which proteoglycan comprises at least one heparan sulfate attachment sequence represented by SEQ ID NO: 15, said heparan sulfate attachment sequence having covalently attached thereto a heparan sulfate glycosaminoglycan chain, wherein said proteoglycan is isolatable as a soluable form only upon the cleavage of a secretion signal peptide. (google.es)
  • ID NO:. 2 ranging between a fragment represented by Gln23Gly50 to a fragment represented by Gln23-Gln210, and a heparan sulfate glycosaminoglycan chain covalently linked thereto which syndecan is isolated free of any biological macromolecules which are present in a natural source of the proteolglycan. (google.es)
  • In addition, we found that although syndecan-1 interacts exclusively through its glycosaminoglycan chains, syndecan-4 binding relies on both its core protein and its heparan sulfate chains. (cf.ac.uk)
  • The extracellular functions of syndecans are primarily mediated by their heparan sulfate (HS) glycosaminoglycan (GAG) side chains, which have affinity for a wide variety of secreted molecules and extracellular matrix (ECM) components. (jneurosci.org)
  • Spreading on the antibody is independent of the heparan sulfate glycosaminoglycan chains and can be inhibited by competition with soluble mAb 281.2. (rupress.org)
  • Differentially expressed patterns of glycosaminoglycan structure in heparan sulfate proteoglycans and free chains. (openrepository.com)
  • Heparan sulphate (HS) 1 is a member of the glycosaminoglycan family of sugars and is present in almost all metazoan organisms. (go.jp)
  • Proteoglycans consist of a core protein, such as a syndecan family protein, to which glycosaminoglycan is linked. (biomedcentral.com)
  • The glypicans contain an N-terminus signal sequence, followed by a globular domain containing multiple cysteine residues, a glycosaminoglycan attachment domain, and a C-terminus that results in the formation of the GPI anchor to the cell surface. (mildredveitia.com)
  • Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes. (deepdyve.com)
  • Structurally diverse side chains add considerably to the size of the core proteins and serve as binding sites for growth factors, cytokines, and extracellular matrix proteins. (rndsystems.com)
  • Syndecans are type I transmembrane proteoglycans and have 3 major domains: an extracellular domain containing heparan sulfate chains, a transmembrane domain, and a short cytoplasmic domain. (medsci.org)
  • they mediate these processes in normal tissues through the binding of heparan sulfate chains to ECM molecules and other effectors, including growth factors, cytokines, proteinases, and proteinase inhibitors [ 1 - 3 ]. (medsci.org)
  • These results suggest that a TNIIIA2-related matricryptic site of the TN-C molecule, exposed by MMP-2 processing, may have bound to syndecan-4 via its HS chains and then induced conformational change in beta1 integrin necessary for its functional activation. (sigmaaldrich.com)
  • proteins with covalently attached, highly acidic sugar chains), unique in their ability to bind many extracellular peptides, such as hormones and growth factors. (jci.org)
  • High expressing transfectants (Raji-Sl cells) bind to and spread on immobilized thrombospondin or fibronectin, which are ligands for the heparan sulfate chains of the proteoglycan. (rupress.org)
  • Cell surface syndecan-1 on distinct cell types differs in fine structure and ligand binding of its heparan sulfate chains. (openrepository.com)
  • Because heparan sulfate can represent several distinct proteoglycans, we asked whether the structural variation in the heparan sulfate chains of a single species of cell surface proteoglycan is a reproducible, differentiated characteristic and whether the variation can result in distinct biological functions. (openrepository.com)
  • Syndecan-1 from these cell types varied in molecular mass largely due to variation in the length of the HS chains. (openrepository.com)
  • Although the highly sulfated and N-acetylated domains in these HS chains were organized similarly, the number of highly sulfated domains differed. (openrepository.com)
  • These structural variations correlated with cell type-specific differences in the affinity of syndecan-1 and its isolated HS chains for type I collagen. (openrepository.com)
  • The results indicate that the size, fine structure, and ligand affinity of the HS chains on a single proteoglycan species differ in a consistent and reproducible manner between cell types. (openrepository.com)
  • Heparan sulfate chains from glypican and syndecans bind the Hep II domain of fibronectin similarly despite minor structural differences. (openrepository.com)
  • Multiple heparan sulfate chains are required for optimal syndecan-1 function. (nih.gov)
  • The specific functions of these molecules are determined by both the core protein and the structure of the attached heparan sulfate chains. (go.jp)
  • The present study reveals the surprising finding that targeting of syndecan-1 to uropods is mediated by its heparan sulfate chains and that targeting is regulated by cell surface events rather than solely by intracellular mechanisms. (uab.edu)
  • The addition of exogenous heparin or the treatment of polarized cells with heparitinase initiates a rapid and dramatic redistribution of uropod syndecan-1 over the entire cell surface, and a mutated syndecan-1 lacking heparan sulfate chains fails to concentrate within uropods. (uab.edu)
  • The syndecan-1 molecules from both lines are similar in size, have indistinguishable core proteins, and have similarly sized heparan sulfate chains. (semanticscholar.org)
  • Transmembrane heparan sulfate proteoglycans ( Syndecan-1 , Syndecan-2 and Syndecan-4 ) are able to bind FGF2 to heparan sulfate chains and present it to the FGFR1 . (bio-rad.com)
  • Remodeling of heparan sulfate chains may affect FGF2 signaling [ 3 ], [ 4 ]. (bio-rad.com)
  • Typically, syndecans and other heparan sulfateCcarrying proteins are thought to hole FGFs through their heparan sulfate chains, thereby facilitating FGF-FGFR binding and stabilizing the formation of the receptor-ligand complex (15). (antibodyassay.com)
  • Syndecans bind and regulate molecules primarily through their HS chains, but do not bind to all HS/heparin-binding molecules. (readbyqxmd.com)
  • GAG chains are long unbranched polymers consisting of many sulfated disaccharides. (biologists.org)
  • Human endosulfatase 1 (HSULF-1) is an enzyme that selectively removes 6-O sulfate groups from HS side chains and alter their level and pattern of sulfation and thus biological activity. (biomedcentral.com)
  • HS side chains are covalently linked to core proteins to form proteoglycans, such as syndecans or glypicans, which associate with or integrate into the lipid bi-layers of cells. (biomedcentral.com)
  • Glycosaminoglycans, in proteoglycan (PG) form or as free chains, play vital roles in every step of tumor progression. (frontiersin.org)
  • In this Paper of the Week, Vishnu Ramani and colleagues find that heparanase, which cleaves heparan sulfate chains, is responsible for enhancing HGF expression in myeloma cells. (pubmedcentralcanada.ca)
  • Table 2 lists several proteoglycans along with their molecular weight, GAG chains, and tissue localization. (sigmaaldrich.com)
  • DNA molecules, localized mainly in cell nuclei, are constructed of a double helix held together by hydrogen bonds between purine and pyramidine bases which project inward from two chains containing alternate links of deoxyribose (a sugar) and phosphate. (ifopa.org)
  • The protein is originally synthesised in an inactive 65 kDa proheparanase form in the golgi apparatus and transferred to late endosomes/lysosomes for transport to the cell-surface. (wikipedia.org)
  • Binding utilizes an enzyme-regulated 'off-on' switch in which active epithelial heparanase (HPSE) cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein. (wikipedia.org)
  • 5) overexpression of syndecan-4 core protein enhanced TNIIIA2-induced activation of beta1 integrin. (sigmaaldrich.com)
  • However, treatments that targeted the cytoplasmic region of syndecan-4, including ectopic expression of its mutant truncated with the cytoplasmic domains and treatment with protein kinase Calpha inhibitor Gö6976, did not influence the TNIIIA2 activity. (sigmaaldrich.com)
  • Regulated expression and subcellular localization of syndecan heparan sulfate proteoglycans and the syndecan-binding protein CASK/LIN-2 during rat brain development. (semanticscholar.org)
  • CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with caskin 1, a novel CASK-binding protein. (semanticscholar.org)
  • Cloning and characterization of hdlg: the human homologue of the Drosophila discs large tumor suppressor binds to protein 4.1. (semanticscholar.org)
  • Cell surface proteoglycans syndecan-1 and -4 bind overlapping but distinct sites in laminin α3 LG45 protein domain. (cf.ac.uk)
  • Tyrosine phosphorylation and protein expression of the T-cell receptor ζ chain (ζ) have been reported to be significantly decreased in SLE T cells. (springer.com)
  • These ζ mRNA splice variant forms exhibit a reduction in the expression of TCR/CD3 complex and ζ protein on their cell surface due to the instability of ζ mRNA splice variant forms as well as the reduction in interleukin (IL)-2 production after stimulating with anti-CD3 antibody. (springer.com)
  • Davies EJ, Tikly M, Wordsworth BP et al (1998) Mannose-binding protein gene polymorphism in South African systemic lupus erythematosus. (springer.com)
  • In addition to binding to polypeptide signaling factors, HS also binds to ECM components including fibronectin, collagen, and laminin and to the cell surface protein neural CAM. (jneurosci.org)
  • BACKGROUND: Clara cell protein (CC16), the main secretory product of bronchiolar Clara cells, plays an important protective role in the respiratory tract against oxidative stress and inflammation. (biomedsearch.com)
  • This study therefore provides a rare direct demonstration of protein conformational change at the cell surface. (exeter.ac.uk)
  • This binding and spreading as not dependent on the cytoplasmic domain of the core protein, is mutants expressing core proteins with cytoplasmic deletions maintain the ability to spread. (rupress.org)
  • The spreading is mediated through engagement of the syndecan-1 core protein, as the Raji-S 1 cells also bind to and spread on immobilized mAb 281.2, an antibody specific for the ectodomain of the syndecan-1 core protein. (rupress.org)
  • These data suggest that the core protein of syndecan-1 mediates spreading through the formation of a multimolecular signaling complex at the cell surface that signals cytoskeleton reorganization. (rupress.org)
  • This complex may form via intramembrane or extracellular interactions with the syndecan core protein. (rupress.org)
  • This loose attachment is followed by a more intimate association involving integral outer membrane protein adhesins, including the colony opacity-associated (Opa) proteins on the bacterial surface [3] , [4] . (pubmedcentralcanada.ca)
  • We have found that the VAP-1 protein expression is significantly decreased in intratumoral vessels compared to peritumoral ones which was independent of the tumor thickness. (aacrjournals.org)
  • Loss of VAP-1 protein expression occured both in endothelial and smoooth muscle cell components. (aacrjournals.org)
  • Unlike in other cancer type, VAP-1 protein expression of intratumoral vessels did not correlate to the density of CTLs or dendritic cells. (aacrjournals.org)
  • Effects of angiogenic factors on CD44v3 expression were studied in vitro both at mRNA and protein level using human umbilical vein (HUVEC), human brain endothelial cells (HBE) and KS-Imm cells. (aacrjournals.org)
  • By proteolytic cleavage of its core protein, syndecan-1 is released from the cell membrane to the extracellular space. (myeloma-europe.org)
  • The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. (mybiosource.com)
  • Interestingly, the heparan sulfate-bearing proteoglycans glypican-1 and betaglycan fail to concentrate in uropods, indicating that targeting may require heparan sulfate structural motifs unique to syndecan-1 or that the core protein of syndecan-1 participates in specific interactions that promote heparan sulfate-mediated targeting. (uab.edu)
  • The technique connects the small molecule that selectively binds to a target protein to a CRL E3 via a chemical linker to trigger the degradation of target protein. (bvsalud.org)
  • Clone REA640 recognizes the mouse syndecan-4 antigen, also known as SYND4 or ryudocan core protein. (miltenyibiotec.com)
  • The process involves the lipid-anchored docking protein Fibroblast growth factor receptor substrate 2 ( FRS2 ) that constitutively binds FGFR1 even when the receptor is not activated. (bio-rad.com)
  • Phosphorylated FRS2 binds the adapter protein Growth factor receptor bound 2 ( GRB2 ) and the Protein tyrosine phosphatase, non-receptor type 11 ( SHP-2 ). (bio-rad.com)
  • That results in activation of the Mitogen-activated protein kinase kinases 1 and 2 ( MEK1/2 )/ Mitogen-activated protein kinases 1 and 3 ( ERK1/2 ) signaling cascade. (bio-rad.com)
  • GRB2 is bound to tyrosine-phosphorylated FRS2 , and the C-terminal SH3 domain of GRB2 forms a complex with the proline-rich region of GRB2-associated binding protein 1 ( GAB1 ) to serve as an interface between these two docking proteins. (bio-rad.com)
  • In the case of FGFR1 signaling, receptor activation occurs at the cell membrane upon ligand binding (15), although intracellular activation of the mitogen-activated protein kinase (MAPK) pathway has also been reported (22). (antibodyassay.com)
  • Zusätzlich bieten wir Ihnen Syndecan 3 Kits (21) und Syndecan 3 Proteine (7) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Hagglund, Van Sant, Lopez, Roizman: Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes. (antikoerper-online.de)
  • We found that when a site-specific binding protein interacts with the "handle" region of the prorenin prosegment, the prorenin molecule undergoes a conformational change to its enzymatically active state. (jci.org)
  • This nonproteolytic activation is completely blocked by a decoy peptide with the handle region structure, which competitively binds to such a binding protein. (jci.org)
  • The particle interacts with the cell surface via interaction of the major capsid protein, L1, with heparan sulfate proteoglycans. (biomedcentral.com)
  • Moreover, accumulating evidence suggests the involvement of a secondary receptor and a possible role for the minor capsid protein, L2, in cell surface interactions. (biomedcentral.com)
  • Protein levels and signaling activities of Hh, Dpp and Wg were reduced in the cells mutant for any of these EXT genes to a various degree, Wg signaling being the least sensitive. (biologists.org)
  • It is pathologically characterized by intracellular neurofibrillary tangles as the result of hyperphosphorylated microtubule-associated protein tau accumulation, as well as extracellular amyloid deposits, called senile plaques ( 1 ), with a progressive accumulation of insoluble amyloid in brain parenchyma and vasculature. (springer.com)
  • The presence of a protein layer on the endothelium was first proposed by Danielli in 1940 [ 1 ] and was visualised using electron microscopy in 1966 [ 2 ]. (biomedcentral.com)
  • Essential for the viability of the surface epithelium of the eye and for normal vision is the thin, but protein-rich, tear film in which the small tear glycoprotein lacritin appears to play a prominent prosecretory, cytoprotective, and mitogenic role. (arvojournals.org)
  • The GAG-protein interactions serve to regulate the signal output of growth factor receptor tyrosine kinase and hence cell fate as well as the storage and diffusion of extracellular protein effectors. (openrepository.com)
  • A prominent carrier protein for the type of glycans implicated in LTP regulation in the adult hippocampus was identified as N -syndecan (syndecan-3), a transmembrane proteoglycan that was expressed at the processes of the CA1 pyramidal neurons in an activity-dependent manner. (jneurosci.org)
  • A proteoglycan is composed of a core protein with one or more covalently attached GAGs. (sigmaaldrich.com)
  • Fibulin-1 is an extracellular matrix protein often associated with fibronectin (FN) in vivo. (biologists.com)
  • MSCs are described by their capability to differentiate to osteoblasts, adipocytes and chondroblasts, by plastic material adherence and by a particular reflection design of specific surface area protein (8,9). (immune-source.com)
  • Using Chinese hamster ovary cells expressing the human syndecan 1 core protein or a chimaeric receptor, FcR-Synd, consisting of the ectodomain of the IgG Fc receptor Ia linked to the transmembrane and cytoplasmic domains of syndecan 1, we previously reported that efficient internalization is triggered by ligand clustering, requires intact actin microfilaments and tyrosine kinases, proceeds with a t 1/2 of approx. (biochemj.org)
  • PBB Summary section title = summary text = The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. (enacademic.com)
  • SDCBP - Syndecan binding protein (syntenin), also known as SDCBP, is a human gene. (enacademic.com)
  • Periostin possessed adjacent domains that bind to tenascin-C and the other ECM protein: fibronectin and type I collagen, respectively. (genes2cognition.org)
  • Syndecan-3, also called SYND3 is a protein that in humans is encoded by the SDC3 gene. (bosterbio.com)
  • The PV particles are composed of 360 copies of the major capsid protein, L1, organized into pentameric capsomers ( 1 , 19 , 27 ), and a less well defined number of the minor capsid protein, L2 ( 2 , 34 , 46 ). (asm.org)
  • The minor capsid protein, L2, does not contribute to the initial binding of papillomaviruses to the cell surface. (asm.org)
  • The major component protein of a cage-like structure that is important in brining proteins from the outside to the inside of a cell. (ifopa.org)
  • Yet, most of this proteoglycan was expressed at the basolateral surface, an unexpected finding for a glypiated protein. (rupress.org)
  • Heat-shock protein 27 and arsenite translocating factor, both involved in actin organization and polymerization, were also increased in the HS-stimulated cells. (biomedcentral.com)
  • Thus, the reduced expression of HA and TGF-β 1 , both important in the development of fibrosis, seems to be mediated by pecific changes in protein expression of the fibroblast. (biomedcentral.com)
  • In a membrane stripe assay in which Hb neurons were allowed to grow across cell membranes from HEK 293 cells transfected with either GFP (green fluorescent protein) or Sema5A, the neurons showed a preference for growing across the Sema5A substrate, which suggests that Sema5A acted as an attractive guidance cue. (sciencemag.org)
  • NHERF1 or EBP50 is a scaffolding protein that functions in apical organization of polarized cells. (sdbonline.org)
  • SDN-1 is the predominant heparan sulfate (HS) proteoglycan in the early C. elegans embryo, and that loss of HS biosynthesis or of the SDN-1 core protein results in misorientation of the spindle of the ABar blastomere. (sdbonline.org)
  • Syndecan-1 is a cell surface proteoglycan containing a highly conserved transmembrane and cytoplasmic domain, and an extracellular domain bearing heparan sulfate glycosaminoglycans. (rupress.org)
  • The major goal of the Park Laboratory is to understand how components of the extracellular matrix (ECM) modulate the pathogenesis of infectious and non-infectious inflammatory diseases, with a particular focus on the role of proteoglycans, glycosaminoglycans, and elastin in these processes. (childrenshospital.org)
  • There are two putative heparin-binding domains (HBDs) within the OPN molecule, which may bind both heparin and heparin-like glycosaminoglycans such as syndecan. (rupress.org)
  • The glycocalyx is also comprised of secreted proteoglycans, such as perlecan, together with secreted glycosaminoglycans, such as hyaluronan. (biomedcentral.com)
  • Heparan sulfates (HSs) constitute a group of glycosaminoglycans that accumulate in the β-amyloid deposits in Alzheimer's disease and influence the development of neuron-target contacts by interacting with other cell surface and matrix molecules. (jneurosci.org)
  • Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. (wikipedia.org)
  • Crystal structures of both proheparanase and mature heparanase are available, showing that the linker peptide forms a large helical domain which blocks heparan sulfate molecules from interacting with heparanase. (wikipedia.org)
  • Heparanase has endoglycosidase activity and cleaves polymeric heparan sulfate molecules at sites which are internal within the polymeric chain. (wikipedia.org)
  • The syndecans are a family of cell-surface heparan sulphate proteoglycans that regulate cell behaviour by binding extracellular matrix molecules such as growth factors. (nih.gov)
  • There are two putative heparin-binding domains (HBDs) within OPN, and one domain overlaps the consensus thrombin cleavage site within OPN molecules (Fig. S1, available at http://www.jem.org/cgi/content/full/jem.20071324/DC1 ) ( 17 ). (rupress.org)
  • Syndecan-4, when bound to OPN, masks not only thrombin cleavage but also integrin binding sites within OPN molecules, thus regulating various OPN functions. (rupress.org)
  • The present study investigates the influence of Syndecan-1 a heparan sulfate proteoglycan binding and modyfying these cell surface molecules on the leukocyte-endothelial interaction. (dog.org)
  • MM cells also produce proteolytic enzymes (uPA, MMP-2, MMP-7, and MMP-9), molecules that can degrade matrix fibers and are likely to promote dissemination of MM cells throughout the bone marrow. (myeloma-europe.org)
  • Among these are cell-surface proteoglycans and integrins, which collaborate extensively in mediating binding of cells to extracellular matrix molecules fibronectin and vitronectin. (vtt.fi)
  • Synthetic molecules that protect cells from anoikis and their use in cell transplantation. (nih.gov)
  • In addition to syndecan-1, several other cell surface molecules localize specifically to the uropod, including CD44 and CD54. (uab.edu)
  • These findings suggest functional specificity for syndecan-1 within uropods and, in addition, reveal a novel mechanism for the targeting of molecules to discrete membrane subcellular domains via heparan sulfate. (uab.edu)
  • However, little is known about the molecules present in Schwann cell processes that could mediate axoglial interactions. (biomedcentral.com)
  • However, little is known about the molecules present in Schwann cells processes that could mediate the organization of axonal nodal proteins. (biomedcentral.com)
  • The glycocalyx is connected to the endothelium via several cell-bound core molecules, mainly proteoglycans and glycoproteins (Fig. 1 ). (biomedcentral.com)
  • Binding of HSs to several molecules implicated in synaptic plasticity suggests that HSs might play a key role in the regulation of brain function. (jneurosci.org)
  • However, instead of simply being a passive, mechanical support for cells, it is in fact an extraordinarily complex scaffold composed of a variety of biologically active molecules that are highly regulated and critical for determining the action and fate of the cells that it surrounds 4 . (sigmaaldrich.com)
  • More recently they have been shown to be active signaling molecules whose roles in a variety of cellular processes (including cytokine production, leukocyte recruitment and inflammatory response) are important for controlling cell fate 7 . (sigmaaldrich.com)
  • These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier. (rupress.org)
  • Heparan sulfate proteoglycans: intricate molecules with intriguing functions. (biomedcentral.com)
  • Searching for a better understanding of cancer, as well as for tumor markers, proteoglycans (PGs) have gained ground among the molecules involved in tumorigenesis. (biomedcentral.com)
  • Unlike the syndecans which can make direct contact with internal cytoskeletal components to activate signal transduction pathways, glypican activation of signaling pathways must be indirect and involve other transmembrane molecules, since glypican does not contain a transmembrane domain. (mildredveitia.com)
  • 2. A soluble portion of the proteoglycan of claim 1, comprising at least one heparan sulfate glycosaminogtycan chain. (google.es)
  • 8. A purified, soluble portion of a mammalian syndecan, comprising a core polypeptide having an amino acid sequence corresponding to a fragment of SEQ. (google.es)
  • Soluble HS fragments produced by HPSE have already been proven to promote toll-like receptor (TLR) 4 signaling in dendritic cells (68) and human being peripheral bloodstream monocytes (69). (lecollege.org)
  • When measured in serum from peripheral blood, concentration of soluble syndecan-1 correlates strongly with a negative outcome for MM patients. (myeloma-europe.org)
  • In a MM mouse model, malignant cells that were genetically transfected to secrete soluble syndecan-1 led to more rapid and severe disease than cells with predominantly surface-expressed syndecan-1. (myeloma-europe.org)
  • Syndecan-4 belongs to the cell surface syndecan proteoglycan family, which binds a variety of soluble and insoluble extracellular effectors. (miltenyibiotec.com)
  • Decoy receptor 3 (DcR3), a soluble receptor for Fas ligand, LIGHT (homologous to lymphotoxins shows inducible expression and competes with HSV glycoprotein D for herpes virus entry mediator, a receptor expressed by T lymphocytes), and TNF-like molecule 1A, is highly expressed in cancer cells and in tissues affected by autoimmune disease. (ox.ac.uk)
  • Enzymatic cleavage of HS by heparitinase as well as addition of soluble heparin-type carbohydrates prevented expression of LTP in response to 100 Hz/1 sec stimulation of Schaffer collaterals in rat hippocampal slices. (jneurosci.org)
  • Addition of soluble N -syndecan into the CA1 dendritic area prevented tetanus-induced LTP. (jneurosci.org)
  • Equimolar amounts of soluble syndecan-4 ectodomain, in contrast, had no effect on this binding. (rupress.org)
  • Our results provide both genetic and biochemical evidence that syndecan-1 can modulate Wnt signalling, and is critical for Wnt-1-induced tumorigenesis of the mouse mammary gland. (nih.gov)
  • Syndecan-1 (SDC1) is reported to modulate several key processes of tumorigenesis and has variable expression in many cancers. (medsci.org)
  • Syndecans are a family of membrane-bound heparan sulfate proteoglycans that modulate ligand-receptor interactions. (jci.org)
  • Here, syndecan ectodomains modulate inflammatory mediators and cells to attenuate inflammatory tissue injury. (childrenshospital.org)
  • Therefore, it is of importance to examine whether syndecan-4 can associate with OPN and modulate OPN function. (rupress.org)
  • In addition, two ancillary proteins, mahogany and syndecan-3, have been found that modulate the activity of the melanocortin peptides (melanocortins). (physiology.org)
  • Aspects of syndecan biology that have attracted research interest include extracellular matrix binding, outside-to-inside plasma membrane signal propagation, activation of cytoplasmic signals, and shedding of the syndecan extracellular domain, but the mechanisms by which syndecan cytoplasmic signals modulate extracellular function remain largely unresolved. (sciencemag.org)
  • CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. (exbio.cz)
  • Syndecan-1/CD138 (Sdc1) is a cell surface heparan sulphate proteoglycan that is highly expressed by epithelial and plasma cells. (biomedcentral.com)
  • CD138 mediates selection of mature plasma cells by regulating their survival. (childrenshospital.org)
  • Of special interest is syndecan-1 (CD138), a heparan sulfate proteoglycan abundantly expressed on the surface of MM cells. (myeloma-europe.org)
  • MBS755256 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Syndecan-1/CD138 (SDC1/CD138) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Gharbaran R. Advances in the molecular functions of syndecan-1 (SDC1/CD138) in the pathogenesis of malignancies. (springer.com)
  • Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. (exbio.cz)
  • The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. (exbio.cz)
  • Surface staining of CD138 in human peripheral blood lymphocytes with anti-CD138 ( B-A38 ) Pacific Orange TM . (exbio.cz)
  • Here we have examined the role of syndecan-1 (encoded by Sdc1) during mammary tumour formation in response to the ectopic expression of the proto-oncogene Wnt1. (nih.gov)
  • We show that in Sdc1-/- mice, Wnt-1-induced hyperplasia in virgin mammary gland was reduced by 70%, indicating that the Wnt-1 signalling pathway was inhibited. (nih.gov)
  • Of the 39 tumours that developed in a test cohort of mice, only 1 evolved in the Sdc1-/- background. (nih.gov)
  • Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1). (wikipedia.org)
  • SDC1 expression was identified in the cancer cells of 212 (96.8%) colon cancer cases. (medsci.org)
  • SDC1 can influence tumorigenesis by regulating the molecular mediators of tumor cell survival, proliferation, angiogenesis, and metastasis [ 1 ]. (medsci.org)
  • Our findings indicate that as B cells differentiate into plasma cells they undergo a coordinated change in chemokine responsiveness that regulates their movements in secondary lymphoid organs and promotes lodgment within the bone marrow. (rupress.org)
  • In particular, syndecans have been implicated in modulation of the activity of the melanocortin system, which potently regulates energy intake, energy expenditure, and peripheral glucose metabolism. (jci.org)
  • Syndecan-1 Regulates Psoriasiform Dermatitis by Controlling Homeostasis of IL-17-Producing ?d T Cells. (childrenshospital.org)
  • However, the molecular mechanism by which syndecan-4 regulates complex host defense responses remains to be elucidated. (rupress.org)
  • Heparan sulfate regulates targeting of syndecan-1 to a functional domain on the cell surface. (nih.gov)
  • Fine structure of heparan sulfate regulates syndecan-1 function and cell behavior. (semanticscholar.org)
  • Since syndecan-1 binding is necessary for lacritin mitogenic and cytoprotective activities, TGM2 cross-linking negatively regulates lacritin bioactivity. (arvojournals.org)
  • Perlecan has multiple functions in cell growth and differentiation and tissue homeostasis. (go.jp)
  • FGFR1 interacts with components of the extracellular matrix, in particular heparan sulfate proteoglycans (such as Perlecan ). (bio-rad.com)
  • Perlecan binds the clustering molecule gliomedin and enhances clustering of node of Ranvier components. (rupress.org)
  • Progenitor, immature, and mature B cells express the chemokine receptor CXCR4, and its ligand, CXCL12 (previously called stromal cell-derived factor [SDF]-1), is highly expressed by bone marrow stromal cells ( 18 )( 19 ). (rupress.org)
  • Mature B cells also express CCR7, a receptor for the T zone chemokines CCL19 (ELC) and CCL21 (SLC), and they are able to migrate to these chemokines ( 17 ). (rupress.org)
  • In Caenorhabditis elegans, mutations in the lin-2 gene inactivate the LET-23 receptor tyrosine kinase/Ras/MAP kinase pathway required for vulval cell differentiation. (semanticscholar.org)
  • Another 16 genes were up-regulated and included genes associated with membranous proteins and cell damage granules, including the genes encoding poliovirus-receptor-related 2, syndecan-1, and granzyme A. (springer.com)
  • Samelson LE, Davidson WF, Morse HC 3rd et al (1986) Abnormal tyrosine phosphorylation on T-cell receptor in lymphoproliferative disorders. (springer.com)
  • Correlation of in situ mechanosensitive responses of the Moraxella catarrhalis adhesin UspA1 with fibronectin and receptor CEACAM1 binding. (exeter.ac.uk)
  • Syndecan-4 is a member of the syndecan family and is an important constituent of host defense mechanisms by acting as a regulatory receptor that monitors dynamic changes in ECM composition and organization during tissue injury and remodeling ( 2 - 6 ). (rupress.org)
  • Among several functions, syndecan-1 adheres MM cells to neighboring cells and acts as a co-receptor for several heparin-binding cytokines. (myeloma-europe.org)
  • Gb3 (Gal α1-4, Gal β1-4, Glc β1-1, Ceramide) is a neutral glycosphingolipid specifically expressed in several human tissues, and is recognised as a receptor for Shiga toxins. (uio.no)
  • FGF2 induces biological responses by binding to and activating Fibroblast growth factor receptor 1 ( FGFR1 ), a subfamily of cell surface receptor tyrosine kinases (RTKs). (bio-rad.com)
  • Isolation of a neuronal cell surface receptor of heparin binding growth-associated molecule (HB-GAM). (google.it)
  • As enveloped viruses, the DENVs enter the cells through receptor mediated endocytosis [ 4 - 7 ] and rearrange cell internal membranes to establish specific sites of replication [ 8 - 10 ]. (hindawi.com)
  • Analogous to specialized cell membrane microenvironments that facilitate the formation of signaling complexes and receptor activation [such as cholesterol and sphingolipid-enriched lipid rafts (19)], cytoplasmic signaling is usually similarly thought to occur at specialized signaling storage compartments (20, 21). (antibodyassay.com)
  • Syndecan-3 may directly transduce Glial cell line-derived neurotrophic factor (GDNF ) family ligands (GFL) signals or serve as a coreceptor, presenting GFLs to the signaling receptor RET . (antikoerper-online.de)
  • The entry of HPV in vitro is initiated by binding to a cell surface receptor in contrast to the in vivo situation where the basement membrane has recently been identified as the primary site of virus binding. (biomedcentral.com)
  • Binding of HPV triggers conformational changes, which affect both capsid proteins L1 and L2, and such changes are a prerequisite for interaction with the elusive uptake receptor. (biomedcentral.com)
  • These observations in association with the changes in FGF gene expression and in the FGF receptor number, suggest that cellular mechanisms downstream from FGF receptor binding are involved in the two different forms of AD. (springer.com)
  • FGF-2 signaling, for instance, requires the N- and 2-O-sulfate groups of heparan sulfate for binding to FGF-2 ligands and the presence of 6-O-sulfate groups for the activation of FGF receptor-1 (FGFR-1). (biomedcentral.com)
  • Human papillomaviruses bind a basal extracellular matrix component secreted by keratinocytes which is distinct from a membrane-associated receptor. (microbiologyresearch.org)
  • Keratinocyte-secreted laminin 5 can function as a transient receptor for human papillomaviruses by binding virions and transferring them to adjacent cells. (microbiologyresearch.org)
  • The HGF and shed syndecan-1 form an active complex that stimulates cell signaling via the proto-oncogenic c- met HGF receptor. (pubmedcentralcanada.ca)
  • When a Notch receptor interacts with an adjacent Notch ligand or one present on an opposing cell, the Notch receptor undergoes a sequence of proteolytic cleavages that release the Notch intracellular domain (NICD). (rupress.org)
  • The formation of distinctive basic FGF-heparan sulfate complexes is essential for the binding of bFGF to its cognate receptor. (rupress.org)
  • In previous experiments, cell-surface heparan sulfate proteoglycans extracted from human lung fibroblasts could not be shown to promote high affinity binding of bFGF when added to heparan sulfate-deficient cells that express FGF receptor-1 (FGFR1) (Aviezer, D., D. Hecht, M. Safran, M. Eisinger, G. David, and A. Yayon. (rupress.org)
  • Cells cotransfected with receptor and proteoglycan showed a two- to three- fold increase in neutral salt-resistant specific 125I-bFGF binding in comparison to cells transfected with only receptor or cells cotransfected with receptor and anti-syndecan-4. (rupress.org)
  • Exogenous heparin enhanced the specific binding and affinity cross-linking of 125I-bFGF to FGFR1 in receptor transfectants that were not cotransfected with proteoglycan, but had no effect on this binding and decreased the yield of bFGFR cross-links in cells that were cotransfected with proteoglycan. (rupress.org)
  • Receptor-transfectant cells showed a decrease in glycophorin A expression when exposed to bFGF. (rupress.org)
  • Finally, complementary cell-free binding assays indicated that the affinity of 125I-bFGF for an immobilized FGFR1 ectodomain was increased threefold when the syndecan-4 ectodomain was coimmobilized with receptor. (rupress.org)
  • We used in vitro binding assays, including surface plasmon resonance, to examine interactions between mutated LG45 and heparan sulfates, syndecan-1 and -4. (cf.ac.uk)
  • Bacterial cell surfaces are commonly decorated with a layer formed from multiple copies of adhesin proteins whose binding interactions initiate colonization and infection processes. (exeter.ac.uk)
  • Syndecan-1 may be a target for prevention of leukocyte-endothelial interactions in angiogenesis and inflammatory diseases of the eye. (dog.org)
  • The role of the microenvironment is now becoming appreciated as a key element in cancer progression, which is driven by interactions between tumor cells and their microenvironment ( 7 - 9 ). (frontiersin.org)
  • We review here the input of gene expression profiling of myeloma cells and of their tumor microenvironment to develop new tumor classifiers, to better understand the biology of myeloma cells, to identify some mechanisms of drug sensitivity and resistance, to identify new myeloma growth factors, and to depict the complex interactions between tumor cells and their microenvironment. (aacrjournals.org)
  • Extracellular domain shedding is believed to play a key role in regulating the link between syndecan-ligand interactions and intracellular signaling. (sciencemag.org)
  • In recent years, there have been significant advances in understanding interactions of DENVs with their target cells such as dendritic cells (DC), macrophages, endothelial cells, and hepatocytes. (hindawi.com)
  • This mini review is intended to provide a concise overview of the literature in HPV virion/host cell interactions and the consequences for endocytosis. (biomedcentral.com)
  • Our data identify S3 and S4 as transmembrane proteins specifically enriched in Schwann cell perinodal processes, and suggest that S3 may be involved in early axoglial interactions during development. (biomedcentral.com)
  • Thus, axoglial interactions generate distinct axonal domains characterized by specific multimolecular complexes (see [ 1 ] for a review). (biomedcentral.com)
  • In addition, GAGs clearly coordinate stromal and epithelial development, and they are active participants in mediating cell-cell and cell-matrix interactions. (openrepository.com)
  • Edwards, 1995 ) (but see also Sorra and Harris, 1998 ), a process that is critically dependent on cell-matrix interactions. (jneurosci.org)
  • Cell-cell interactions of cancer cells with endothelium determine the metastatic spread. (hindawi.com)
  • This review summarizes recent findings about potential mechanisms of tumor cell interactions in the vasculature and antimetastatic activities of heparin. (hindawi.com)
  • Metastasis is facilitated by cell-cell interactions between tumor cells and the endothelium in distant tissues. (hindawi.com)
  • The organ-specific character of metastasis has been already observed by S. Paget more than a century ago, and the "seed and soil" hypothesis postulates specific interactions of tumor cells with the "friendly" environment of distant organs that enables the establishment of metastasis and subsequent growth [ 2 ]. (hindawi.com)
  • a) Schematic presentation of selectin- and integrin-mediated cancer cell interactions with several blood constituents (e.g., platelets, leukocytes, and endothelial cells) during hematogenous metastasis. (hindawi.com)
  • and VLA4-mediated interactions of cancer cells within blood circulation thereby affecting metastasis. (hindawi.com)
  • In addition to elastin and fibrillin-1, over 30 ancillary proteins are involved in mediating important roles in elastic fibre assembly as well as interactions with the surrounding environment. (wikipathways.org)
  • Fibulin-5 for example, is expressed by vascular smooth muscle cells and plays an essential role in the formation of elastic fibres through mediating interactions between elastin and fibrillin (Yanigasawa et al. (wikipathways.org)
  • We conclude that, at least in K562 cells, syndecans and glypican can support bFGF-FGFR1 interactions and signaling, and that cell-surface association may augment their effectiveness. (rupress.org)
  • Increasing evidence indicates that miR-145 is a tumor suppressor capable of inhibiting breast and colon cancer cell growth both in vitro and in vivo. (biomedsearch.com)
  • Cancer metastasis is the dissemination of tumor cells to new sites, resulting in the formation of secondary tumors. (frontiersin.org)
  • Metastasis of a tumor is the systemic dissemination and colonization of tumor cells from the primary tumor to a secondary site and is a major cause of cancer-related deaths ( 1 ). (frontiersin.org)
  • However, although IL-6 is essential, it is not sufficient and additional factors, produced by the microenvironment or the tumor cells themselves, are required together with IL-6 to promote tumor growth ( 3 ). (aacrjournals.org)
  • Yao, Zhang, Huang, Li, Gao: Pleiotrophin and N-syndecan promote perineural invasion and tumor progression in an orthotopic mouse model of pancreatic cancer. (antikoerper-online.de)
  • Hematogenous cancer metastasis is a multistep cascade encompassing process, starting with local invasion of tumor cells at primary tumors, survival in systemic circulation, extravasation in secondary sites, and ending with establishment of growing metastatic lesions. (hindawi.com)
  • The metastatic capacity of tumor cells correlates with their ability to exit from the blood circulation, to colonize distant organs, and to grow in distant organs [ 1 ]. (hindawi.com)
  • Using anti-CS antibodies, normal stroma was always negative whereas tumoral stroma always showed a positive staining, more intense in the highest grade carcinomas, while tumor cells were negative. (frontiersin.org)
  • Moreover, certain specific cell surface PGs experienced a drastic decrease in expression depending on tumor differentiation. (frontiersin.org)
  • HGF is overexpressed by multiple myeloma cells, which aids tumor angiogenesis, cell migration, and cell survival. (pubmedcentralcanada.ca)
  • However, little is known about how this process is regulated in tumor cells. (pubmedcentralcanada.ca)
  • Furthermore, we show that Syndecan-3 interacts with Notch and is required for Notch processing by ADAM17/tumor necrosis factor-α-converting enzyme (TACE) and signal transduction. (rupress.org)
  • In this position, LPL can mediate release of fatty acids from circulating lipoproteins for diffusion-based transport to the adipocyte surface or newly released fatty acids are transported through their association with circulating albumin. (biomedcentral.com)
  • Syndecan heparan sulphate proteoglycans directly mediate a novel endocytic pathway. (biochemj.org)
  • A genetically determined self-destruction of cells that is thought to exist to mediate the orderly elimination of unnecessary cells. (ifopa.org)
  • Syndecan-4 is able to bear heparan sulfate and is an important regulator of bFGF signaling. (miltenyibiotec.com)
  • Targeted cells signal to NFAT and mTOR if conditions are suitable for proliferation, or to AKT and FOXO3 under conditions of stress. (wikipedia.org)
  • Gottlieb AB, Lahita RG, Chiorazzi N et al (1979) Immune function in systemic lupus erythematosus: impairment of in vitro T cell proliferation and in vivo antibody response to exogenous antigen. (springer.com)
  • miRNA-145 inhibits non-small cell lung cancer cell proliferation by targeting c-Myc. (biomedsearch.com)
  • In this study we evaluated whether coexpression of these functionally linked prognostic markers constitutes an expression signature in ductal carcinoma in situ (DCIS) of the breast that may promote cell proliferation and (lymph)angiogenesis. (biomedcentral.com)
  • This coexpression may reflect a state of parallel activation of different signal transduction pathways, promoting tumour cell proliferation and angiogenesis. (biomedcentral.com)
  • Multiple myeloma is a B-cell neoplasia characterized by the proliferation of a clone of malignant plasma cells in the bone marrow. (aacrjournals.org)
  • One key finding to understand the biology of myeloma has been the identification of interleukin-6 (IL-6) as a major survival and proliferation factor for myeloma cells in 1988 ( 1 , 2 ). (aacrjournals.org)
  • They are primarily categorized into three types PIM-1, PIM-2, PIM-3 which plays an indispensable regulatory role in signal transduction cascades, by promoting cell survival, proliferation, and drug resistance. (bvsalud.org)
  • The mass of adipose tissue is maintained by a well-controlled balance of cell proliferation (hyperplasia) and increase in fat cell size (hypertrophy). (biomedcentral.com)
  • Ojeh N, Hiilesvuo K, W rri A, Salmivirta M, Henttinen T, M tt A: Ectopic expression of syndecan-1 in basal epidermis affects keratinocyte proliferation and wound re-epithelialization. (exbio.cz)
  • HSULF-1 over-expression reduced ERK and Akt signaling activation in H292 cells, which further demonstrated its inhibitory effects on signaling related to proliferation. (biomedcentral.com)
  • We hypothesize that HSULF-1 plays important roles in cancer cells and functions to modify cell signaling, inhibit cancer proliferation, and promote cancer cell death. (biomedcentral.com)
  • 1 2 3 4 Epithelial proliferation is initiated within a few hours, and stem cells or stemlike cells within the epithelium may be activated. (arvojournals.org)
  • In the absence of Syndecan-3, satellite cells stall in S phase, leading to reduced proliferation, increased cell death, delayed onset of differentiation, and markedly reduced numbers of Pax7 + satellite cells accompanied by myofiber hypertrophy and an increased number of centrally nucleated myofibers. (rupress.org)
  • In the presence of TN-C, however, acini failed to generate a normal BM, and net epithelial cell proliferation increased. (genes2cognition.org)
  • Thus, TN-C promoted epithelial cell proliferation leading to luminal filling, a process that we hypothesized involved c-met, a proto-oncogene amplified in breast tumors that promotes intraluminal filling. (genes2cognition.org)
  • Indeed, TN-C increased epithelial c-met expression and promoted luminal filling, whereas blockade of c-met function reversed this phenotype, resulting in normal BM deposition, proper lumen formation, and decreased cell proliferation. (genes2cognition.org)
  • The observed inhibition of cell proliferation, HA, and TGF-β 1 allows speculation of highly sulfated HS as a antifibrotic candidate in the early stage of remodeling. (biomedcentral.com)
  • Osteogenic differentiation of mesenchymal stem cells (MSC) is a tightly regulated process, inducing large changes in cell phenotype and gene expression. (uio.no)
  • Since HPV replication and assembly requires infected basal keratinocytes to undergo the stepwise differentiation program of the epithelium [ 19 , 20 ], HPV propagation in cell culture is a major challenge. (biomedcentral.com)
  • Lymphocytes express and lose syndecan at specific stages of differentiation. (springer.com)
  • May take part in the regulation of cell motility, growth and differentiation. (string-db.org)
  • During differentiation of 3T3-L1 pre-adipocytes, LPL mRNA expression increases 6-fold resulting in a 2-fold increase in cell surface-associated LPL enzymatic activity. (biomedcentral.com)
  • The present invention provides tangible means and methods for stimulation of angiogenesis via enhanced endothelial expression of core proteins having a syndecan-4 cytoplasmic region intracellularly. (google.es)
  • Furthermore, the negatively charged, sulfated GAGs of cell surface proteoglycans, but not their core proteins, were identified as the binding sites for DcR3.Fc. (ox.ac.uk)
  • Many different cell surface and matrix proteoglycan core proteins are expressed in the mammary gland and in mammary cells in culture. (openrepository.com)
  • Syndecan - One of a family of core proteins with sugars attached to the cell surfaces that control or influence tissue repair, metabolism, the formation of tumors and the development of immune responses. (enacademic.com)
  • In contrast, cells transfected with the glycosylphosphatidylinositol-anchored proteoglycan glypican-1 do not bind to collagen and remain invasive, even though glypican- and syndecan-expressing cells have similar surface levels of heparan sulfate, and their proteoglycans have similar affinities for collagen. (storysteel.gq)
  • In contrast, normal neuroendocrine cells were positive for glypican 1, displaying intense staining in cytoplasm and membrane. (frontiersin.org)
  • Whilst elevated glypican 1 expression has been documented in different tumors, the downregulation in high-grade tumors observed in this work suggests that this proteoglycan could be involved in cancer development in a more complex and context-dependent manner than previously thought. (frontiersin.org)
  • In alternative tests to establish whether cell-surface proteoglycans can support the formation of the required complexes, K562 cells were first transfected with the IIIc splice variant of FGFR1 and then transfected with constructs coding for either syndecan-1, syndecan-2, syndecan-4 or glypican, or with an antisense syndecan-4 construct. (rupress.org)
  • Domain-specific biotinylation of the apical and basolateral membranes of these cells identified glypican, a GPI-linked heparan sulfate proteoglycan, as the major source of apical heparan sulfate. (rupress.org)
  • Glypican-1 is found in the brain, kidneys, skeletal muscle, and several other tissues (82-84). (mildredveitia.com)
  • Using a modified atomic force microscope, we show that the adhesive properties and thickness of the UspA1 layer at the cell surface varies on addition of either fibronectin or CEACAM1. (exeter.ac.uk)
  • Syndecan-1 Promotes Streptococcus pneumoniae Corneal Infection by Facilitating the Assembly of Adhesive Fibronectin Fibrils. (childrenshospital.org)
  • Bioactive proteins can also be secreted by the MM cells themselves (e.g. fibronectin, HGF, VEGF, TNF, IL-6) and have the potential to act in an autocrine manner. (myeloma-europe.org)
  • Regulatory proteins of the mammary gland that bind GAG include many growth factors and morphogens (fibroblast growth factors, hepatocyte growth factor/scatter factor, members of the midkine family, wnts), matrix proteins (collagen, fibronectin, and laminin), enzymes (lipoprotein lipase) and microbial surface proteins. (openrepository.com)
  • The syndecan family of cell surface heparan sulfate proteoglycans interacts via their cytoplasmic C-terminal tail with the PDZ domain of CASK/LIN-2, a membrane-associated guanylate kinase homolog. (jneurosci.org)
  • Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae) subvert the syndecan family of cell surface heparan sulfate proteoglycans to enhance their virulence in vivo. (childrenshospital.org)
  • The factors that regulate plasma cell localization are poorly defined. (rupress.org)
  • Syndecan-3 is heavily expressed in several areas of the brain, including hypothalamic nuclei, which are known to regulate energy balance. (jci.org)
  • Syndecan-4 is known to regulate thrombin activity ( 18 , 19 ), and OPN function is regulated by thrombin cleavage ( 12 , 14 , 17 , 20 ). (rupress.org)
  • Tissue fluids surrounding a wound contain an abundance of shed syndecan-1 and -4 ectodomains ( 9 ), which are thought to regulate inflammation and protect against tissue damage by modulating chemokine bioavailability. (sciencemag.org)
  • The variety of HS present on cell surfaces and in the matrix in tissues could be one way that organisms regulate GF activity during development and other processes such as wound healing. (go.jp)
  • Inducible expression of the cell surface heparan sulfate proteoglycan syndecan-2 (fibroglycan) on human activated macrophages can regulate fibroblast growth factor action. (ox.ac.uk)
  • A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic. (google.es)
  • The four vertebrate syndecans, Syndecan-1 through -4, have similar short cytoplasmic domains and extracellular portions that diverge, except for HS attachment sites. (rndsystems.com)
  • CASK is a cytoplasmic-binding partner for syndecans, and its subcellular distribution changes strikingly during development, shifting from a primarily axonal distribution in the first 2 postnatal weeks to a somatodendritic distribution in adult brain. (jneurosci.org)
  • The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. (google.es)
  • have now discovered that association between an endocytic regulator, Rab5, and the syndecan-1 cytoplasmic domain controlled the shedding of the syndecan-1 extracellular domain. (sciencemag.org)
  • Historically, the issues of ligand recognition and cytoplasmic signaling by syndecans have been addressed independently and, although there is strong evidence for signal transduction across the membrane ( 6 ), there is surprisingly little known about the mechanism by which intra- and extracellular domain functions are integrated. (sciencemag.org)
  • However, recent studies of the cytoplasmic signaling capabilities of S4 have suggested that there may be additional mechanisms of proteoglycan-mediated rules (1, 16, 17). (antibodyassay.com)
  • The HMR-1 cytoplasmic domain binds catenins and promotes the formation of adherens-junction-like adhesions (reviewed by Cox and Hardin, 2004 ). (biologists.org)
  • Insolubility of clustered FcR-Synd ligand did not require the syndecan ectodomain, linkage of the cytoplasmic tail to the cytoskeleton, or energy-dependent cellular metabolism. (biochemj.org)
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
  • Where studied, HS is known to be structurally heterogeneous and to vary in sulfation pattern between cells and tissues. (openrepository.com)
  • In addition, POMC mRNA and immunoreactivity have been reported in a number of peripheral human tissues, including genitourinary tract, gastrointestinal tract, adrenal, spleen, lung, and thyroid and in cells of the immune system ( 52 ). (physiology.org)
  • Due to their widespread substrate spectrum, MMPs are integrated as important regulators for tissue homeostasis and immunity in the network of multidirectional communication within tissues and cells. (ersjournals.com)
  • HS possesses a great deal of structural diversity that can be altered spatially and temporally in cells and tissues. (go.jp)
  • Syndecan 2 levels were very low or undetectable in healthy tissues, increasing significantly in well-differentiated tumors, and decreasing in poorly differentiated NETs, and its expression levels showed a positive correlation with patient survival. (frontiersin.org)
  • The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. (sigmaaldrich.com)
  • A come cell type that, for quite some right period, is definitely known to apply paracrine results to orchestrate twisted curing is normally the mesenchymal control cell (MSC), a multipotent stromal progenitor cell residing in bone fragments marrow and adipose tissues (6 preferentially,7). (immune-source.com)
  • Here we demonstrate that, compared with their B cell precursors, plasma cells exhibit increased chemotactic sensitivity to the CXCR4 ligand CXCL12. (rupress.org)
  • The identity and character of the dual-ligand combination influenced cell behavior directly. (immune-source.com)
  • Pretreatment of cells with cyclodextrin to deplete cholesterol from rafts abolished insolubility of the clustered ligand and inhibited endocytosis in a dose-responsive fashion. (biochemj.org)
  • Overall, our results indicate a multi-step endocytic process consisting of ligand binding, clustering, energy-independent lateral movement into detergent-insoluble membrane rafts and finally recruitment of actin and tyrosine kinases to bring the ligands into the cell. (biochemj.org)
  • This study highlights the importance of post-translational modifications of host cellular factors in HIV-1 replication and pathogenesis. (medworm.com)
  • Thus, the variation in structure and binding ability of HS on syndecan-1 is a differentiated characteristic of the cell type that can enable cells to respond distinctly to the HS-binding effectors in the cellular microenvironment. (openrepository.com)
  • The extracellular matrix (ECM) is a non-cellular meshwork of crosslinked macromolecules including collagens, proteoglycans, and glycoproteins, that form a dynamic, supramolecular, scaffold. (frontiersin.org)
  • Biochemical and biomechanical cues present in the ECM, such as sequestered growth factors, ECM biomechanics and ultrastructural organization, are sensed by cells and converted into downstream cellular responses. (frontiersin.org)
  • CRLs play vital roles in the regulation of multiple cellular processes, including cell cycle, cell apoptosis, DNA replication, signalling transduction among the others, and are frequently dysregulated in many human cancers. (bvsalud.org)
  • The single open reading frame is directly translated into a polyprotein precursor [ 1 ], which is subsequently glycosylated by cellular glycosyltransferases and cleaved by proteases from virus and host cell to release three structural proteins (envelope glycoprotein (E), membrane (M), and capsid (C)) and seven nonstructural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5). (hindawi.com)
  • Based on its function as an anchor that stabilizes epithelial sheet morphology by connecting the extra cellular matrix (ECM) to intracellular cytoskeleton, this family of proteoglycans was termed as "syndecan," derived from Greek word "syndein" meaning to bind together. (springer.com)
  • Since a single proteoglycan, even if it carries a single GAG chain, can bind multiple proteins, proteoglycans are also likely to act as multireceptors which promote the integration of cellular signals. (openrepository.com)
  • This study investigated how defects in glycoconjugate component of the cell surface of Pax6 +/− could cause the abnormal cellular migration phenotypes associated with the disease. (arvojournals.org)
  • We investigated the molecular and cellular events that lead to altered myogenesis upon genetic ablation of Syndecan-3, a component of the satellite cell niche. (rupress.org)
  • A cellular structure that is involved in the transport of proteins from the outside to the inside of the cell. (ifopa.org)
  • These four GAGs are typically covalently attached to proteins to form proteoglycans. (sigmaaldrich.com)
  • In addition, ζ mRNA with alternatively spliced 3′ untranslated region (ζmRNA/as-3′UTR) is detected predominantly in SLE T cells, and aberrant ζ mRNA accompanied by the mutations in the open reading frame including ζ mRNA lacking exon7 (ζmRNA/exon7-) is observed in SLE T cells. (springer.com)
  • However, multimerisation predominantly occurs outside the cell. (wikipathways.org)
  • We studied the molecular structure and binding affinity for type I collagen and fibroblast growth factor-2 of syndecan-1 purified from the surfaces of NMuMG normal murine mammary gland epithelia, NIH/3T3 fibroblasts, and BALB/3T3 endothelioid cells. (openrepository.com)
  • Monocyte/macrophages play important roles in regulating tissue growth and angiogenesis through the controlled release of heparin-binding growth factors such as fibroblast growth factor (FGF), vascular endothelial growth factor, and heparin binding epidermal growth factor. (ox.ac.uk)
  • S4 signals largely through its short intracellular domain name, which includes a C-terminal PDZ (postsynaptic density, Disks large, zona occludens 1) binding region in one of its two conserved domain names (4). (antibodyassay.com)
  • This PDZ-binding domain name binds numerous intracellular partners, including synectin (5). (antibodyassay.com)
  • Exogenous addition of purified LPL (to restore extracellular lipolytic activity) or palmitate (as a source of free fatty acids) to siRNA-treated cells restored intracellular lipid levels to those measured for non-treated controls. (biomedcentral.com)
  • We show that the aberrant cell cycle and impaired self-renewal of explanted Syndecan-3-null satellite cells are rescued by ectopic expression of the constitutively active Notch intracellular domain. (rupress.org)
  • Endogenous danger signals, or damage-associated molecular patterns (DAMPs), are generated in response to cell stress and activate innate immunity to provide a pivotal mechanism by which an organism can respond to damaged self. (jci.org)
  • In recent years, the study of the molecular mechanisms regulating syndecan function has gained momentum as the library of possible ligands and biological roles has expanded. (sciencemag.org)
  • Irvin, Herold: Molecular mechanisms linking high dose medroxyprogesterone with HIV-1 risk. (antikoerper-online.de)
  • Molecular functions of syndecan-1 in disease. (springer.com)
  • Skeletal muscle postnatal growth and repair depend on satellite cells and are regulated by molecular signals within the satellite cell niche. (rupress.org)
  • Syndecans are type 1 transmembrane proteoglycans that constitute the major physiological form of heparan sulfate (HS) on the cell surface. (rndsystems.com)
  • Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels. (childrenshospital.org)
  • The syndecan transmembrane proteoglycans play critical regulatory roles in many biological processes, including wound healing, inflammation, neural patterning, and angiogenesis ( 1 , 2 ). (sciencemag.org)
  • Using overexpression and knock-down experiments we demonstrated that syndecan-1, but not syndecan-2 or -4, enhanced binding of α2β1 to collagen. (vtt.fi)
  • Finally, crosstalk between syndecan-1 and α2β1 integrin was found to enhance the transcription of matrix metalloproteinase-1 in response to collagen binding. (vtt.fi)
  • Similarly, the incorporation of fibulin-1 into FN-containing type I collagen gels inhibited the invasion of endocardial cushion mesenchymal cells migrating from cultured embryonic heart explants. (biologists.com)
  • Cell surface proteoglycan that bears heparan sulfate. (string-db.org)
  • Proteolytic cleavage of the syndecan extracellular domain at a membrane-proximal site causes accumulation of shed ectodomains that compete with intact syndecans for extracellular ligands. (sciencemag.org)
  • Due to the highly complicated character of the extracellular matrix (ECM), the style and implementation of active, stimuli-responsive surfaces that present well-defined ligands and serve as magic size ECM substrates possess been of huge desire to biomaterials, biosensor, and cell biology neighborhoods. (immune-source.com)
  • A redox-responsive cause was included into this surface area technique to discharge ligands in the existence of adhered cells automatically, and cell dispersing, development, and migration replies had been compared and measured. (immune-source.com)
  • A peptide derived from tenascin-C induces beta1 integrin activation through syndecan-4. (sigmaaldrich.com)
  • A lateral interaction of beta1 integrin with the extracellular region of the syndecan-4 molecule may be involved in this conformation change. (sigmaaldrich.com)
  • When OPN is associated with syndecan-4, syndecan-4 masks both the thrombin cleavage and the integrin binding sites within OPN. (rupress.org)
  • Moreover, syndecan-1 co-localized with α2β1 integrin and contributed to proper organization of cortical actin. (vtt.fi)
  • Ectopic Wnt-1 expression induces generalized mammary hyperplasia, followed by the development of solitary tumours (median time 22 weeks). (nih.gov)
  • A downstream heparan sulphate binding site has been mapped that induces focal adhesions. (biologists.org)
  • Our results indicate that IL-8 is immobilized on the endothelial cell surface through binding to syndecan-1 ectodomains, and that plasmin, generated by endothelial plasminogen activator, induces the shedding of this form of IL-8. (aston.ac.uk)
  • A series of treatments utilizing heparins and heparinase revealed that HPV18 VLPs are dependent on heparan sulfates (HS) for binding to cells and ECM. (microbiologyresearch.org)
  • Here we show that hippocampal LTP is critically dependent on heparan sulfates, and identify N -syndecan as a major heparan sulfate proteoglycan expressed in the pyramidal neurons of hippocampus. (jneurosci.org)
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • Infection with recombinant adenovirus for HSULF-1 over-expression resulted in decreased cell viability in H292 cells, but not in normal hAT2 cells. (biomedcentral.com)
  • Cross-linking was absent when Tgm2 was inactive, and cross-linked lacritin, unlike recombinant monomer, bound syndecan-1 poorly. (arvojournals.org)
  • In vitro , the addition of recombinant heparanase to myeloma cell lines or transfecting them with heparanase cDNA increased the expression and secretion of HGF along with increased synthesis and shedding of the heparan sulfate proteoglycan syndecan-1. (pubmedcentralcanada.ca)
  • Fibrillin-1 is a major heparin-binding molecule. (biologists.org)
  • FGF treatment differently modulated ECM molecule production and gene expression in the two cell populations. (springer.com)
  • All proteins were expressed in mammalian cells, purified, and characterized biochemically. (cf.ac.uk)
  • The PDZ-binding domain name has been implicated in orchestrating endothelial migration through the Rho family guanosine triphosphatases (GTPases) RhoG and Rac1 (7), whereas the conversation between S4 and PKC promotes mTOR (mammalian target of rapamycin) complex 2 assembly and Akt activity (8). (antibodyassay.com)
  • HPV11 VLP binding is blocked when the ECM is pretreated with an anti-laminin 5 (LN5) polyclonal antibody. (microbiologyresearch.org)
  • When polarized myeloma cells are incubated with heparin-binding proteins, like hepatocyte growth factor or osteoprotegerin, they concentrate in the uropod. (uab.edu)
  • The chemistry, biochemistry and pharmacology of heparin and heparan sulfate were and stay an incredible clinical project - heparin and its spinoff stay vital medications in medical perform. (mildredveitia.com)
  • Chemistry and Biology of Heparin and Heparan Sulfate presents readers with an perception into the chemistry, biology and scientific functions of heparin and heparan sulfate and examines their functionality in quite a few physiological and pathological stipulations. (mildredveitia.com)
  • Structural requirements for calmodulin binding to membrane-associated guanylate kinase homologs. (semanticscholar.org)
  • Structural studies of PIM kinases revealed that they have unique hinge regions where two Proline resides and makes ATP binding unique, by offering a target for an increasing number of potent PIM kinase inhibitors. (bvsalud.org)
  • Importantly, their aqueous dispersion has high localized surface plasmon resonance (LSPR) absorbance in near-infrared (NIR) region different from previous report, which possibly associates with the charge transfer and structural distortion in hydrogen rhenium bronze (HxReO3), as well as ReO3's cubic shape. (readbyqxmd.com)
  • Given the structural and functional differences among the endothelium types, one could speculate that the morphology of glycocalyx would also vary among the different types of endothelial cells. (biomedcentral.com)
  • Fibrillin-1 is the major structural component of microfibrils. (wikipathways.org)
  • It has long been understood to be the structural support for cells since its characteristics set the characteristics of the tissue (i.e. bone compared to cartilage compared to brain)1. (sigmaaldrich.com)
  • Detailed structural analysis of HS from cells overexpressing SULF2 showed a reduction of the trisulfated disaccharide UA(2S)-GlcNS(6S). (biomedcentral.com)
  • Once in the active GTP-bound state, H-Ras interacts with several effector proteins, including v-Raf-1 murine leukemia viral oncogene homolog 1 ( c-Raf-1 ). (bio-rad.com)
  • Antibody-secreting plasma cells are nonrecirculatory and lodge in splenic red pulp, lymph node medullary cords, and bone marrow. (rupress.org)
  • Plasma cells play a critical role in humoral immunity by functioning as factories for antibody production. (rupress.org)
  • In the case of T cell-dependent antibody responses, antigen-engaged B cells receive T cell help in the region of the T zone-bordering follicles. (rupress.org)
  • Rabbit IgG polyclonal antibody for Syndecan-3(SDC3) detection. (bosterbio.com)
  • Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017! (bosterbio.com)
  • The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. (wikipedia.org)
  • 5. (twice amended) The proteoglycan of claim 3, which proteoglycan is isolated from an endothelial cell transformed with an expression vector encoding said proteoglycan. (google.es)
  • Neutrophil recruitment is directed by transendothelial gradients of IL-8 that, in vivo, are bound to the endothelial cell surface. (aston.ac.uk)
  • In the heart, the endothelial glycocalyx presented as moss- or broccoli-like and covered the entire luminal endothelial cell surface. (biomedcentral.com)
  • Translocation of this complex to the plasma membrane by binding to phosphorylated FRS2 allows SOS to activate v-Ha-ras Harvey rat sarcoma viral oncogene homolog ( H-Ras ) by GTP exchange due to its close proximity to membrane-bound H-Ras . (bio-rad.com)
  • Paraffin-embedded tissue samples taken from 175 patients with primary SCC, followed up from 2 to 15 years after surgery, were studied for expression of syndecan-1 by immunohistochemistry. (nih.gov)
  • In breast cancer, changes in the expression of syndecan-1, E-cadherin and c-met correlate with poor prognosis. (biomedcentral.com)
  • Expression of syndecan-1, E-cadherin and c-met was detected immunohistochemically using a tissue microarray in tumour specimens from 200 DCIS patients. (biomedcentral.com)
  • Induced expression of syndecan in healing wounds. (biomedcentral.com)
  • and the resulting overexpression of these proteoglycan entities causes stimulation of angiogenesis in-situ. (google.es)
  • Although the activity of MMPs has been shown to be essential in cell biological processes and many fundamental physiological events involving tissue remodelling, such as angiogenesis, bone development, wound healing and mammary involution [ 2 ], the increasing interest in MMP function mainly stems from their role in several pathological conditions, such as cancer or chronic inflammatory diseases [ 3 ]. (ersjournals.com)
  • Lack of Syndecan-1 may attribute to the increased corneal angiogenesis in sdc-/- mice. (dog.org)
  • Despite the well-characterized series of migration events, relatively little has been known about the cues directing plasma cell movements. (rupress.org)
  • Uronyl 2-O sulfotransferase potentiates Fgf2-induced cell migration. (semanticscholar.org)
  • We have investigated the identity and function of the binding site(s) in an in vitro model of neutrophil transendothelial migration. (aston.ac.uk)
  • Increased shedding of IL-8/heparan sulfate/syndecan-1 complexes was accompanied by inhibition of neutrophil transendothelial migration, and aprotinin, a potent plasmin inhibitor, reversed this inhibition. (aston.ac.uk)
  • Platelets, added as an exogenous source of PAI-1, had no effect on shedding of the complexes or neutrophil migration. (aston.ac.uk)
  • Alterations to the cell surface glycoconjugate signature of Pax6 +/ − corneal epithelia restrict the ability of cells to initiate migration in response to wounding. (arvojournals.org)
  • When an epithelium is wounded, a complex series of signaling pathways are initiated in cells radiating from the wound site that promote immediate cell migration into the wound. (arvojournals.org)
  • 10 However, when Pax6 +/ − cells overcome their block, they migrate as fast as wild-type, and so there is no intrinsic defect in cell migration, only in their ability to respond quickly to wounding. (arvojournals.org)
  • Transfected cells were then submitted to viability, migration and colony formation assays. (biomedcentral.com)
  • Furthermore, forced expression of SULF2 augmented cell migration and colony formation in both prostate cell lines. (biomedcentral.com)
  • Its gene is a TATA-less gene, with several transcription initiation sites, which is activated by the binding of Sp1 and acetylated histone H3 (H3) in the core promoter region (CPR) through the derepression of class I histone deacetylase (HDAC). (medworm.com)
  • For example, gene expression profile (GEP) analysis of primary myeloma cells from 92 patients was analyzed according to their immunoglobulin types and light chain subtypes, revealing that several clusters of genes involved in various biological functions could significantly discriminate those different types of myeloma ( 20 ). (aacrjournals.org)
  • SDC3 is a target gene of the BACH1 (zeige BACH1 Antikörper ) transcription factor according to ChIP-seq analysis in HEK (zeige EPHA3 Antikörper ) 293 cells. (antikoerper-online.de)
  • Proteoglycans (PG), metalloprotease (MMP)-1, and FGF gene expressions were measured by reverse transcription-polymerase chain reaction. (springer.com)
  • They consist of four members: syndecan-1, syndecan-2 (fibroglycan), syndecan-3(N-syndecan), and syndecan-4 (amphiglycan/ryudocan) each encoded by a distinct gene (Teng et al. (springer.com)
  • 2007). Fibrillinopathies include Marfan syndrome, familial ectopia lentis, familial thoracic aneurysm, all due to mutations in the fibrillin-1 gene FBN1, and congenital contractural arachnodactyly which is caused by mutation of FBN2 (Maslen & Glanville 1993, Davis & Summers 2012). (wikipathways.org)
  • SDC2 - Syndecan 2, also known as SDC2, is a human gene. (enacademic.com)
  • To further investigate SULF2 role in tumorigenesis, we overexpressed such gene in prostate cancer cell lines. (biomedcentral.com)
  • Swain N., Hosalkar R.M., Routray S. (2018) Syndecan-1. (springer.com)