The parts of a macromolecule that directly participate in its specific combination with another molecule.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The rate dynamics in chemical or physical systems.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Established cell cultures that have the potential to propagate indefinitely.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Proteins prepared by recombinant DNA technology.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.
Nucleic acid sequences involved in regulating the expression of genes.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Proteins found in any species of bacterium.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Transport proteins that carry specific substances in the blood or across cell membranes.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Nucleotide sequences of a gene that are involved in the regulation of GENETIC TRANSCRIPTION.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Deletion of sequences of nucleic acids from the genetic material of an individual.
Measurement of the intensity and quality of fluorescence.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A site on an enzyme which upon binding of a modulator, causes the enzyme to undergo a conformational change that may alter its catalytic or binding properties.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Commonly observed BASE SEQUENCE or nucleotide structural components which can be represented by a CONSENSUS SEQUENCE or a SEQUENCE LOGO.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Biologically active molecules which are covalently bound to the enzymes or binding proteins normally acting on them. Binding occurs due to activation of the label by ultraviolet light. These labels are used primarily to identify binding sites on proteins.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Proteins obtained from ESCHERICHIA COLI.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
The sum of the weight of all the atoms in a molecule.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
Antibodies produced by a single clone of cells.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A computer simulation technique that is used to model the interaction between two molecules. Typically the docking simulation measures the interactions of a small molecule or ligand with a part of a larger molecule such as a protein.
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Organic or inorganic compounds that contain the -N3 group.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
The first nucleotide of a transcribed DNA sequence where RNA polymerase (DNA-DIRECTED RNA POLYMERASE) begins synthesizing the RNA transcript.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
The characteristic three-dimensional shape of a molecule.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Cell surface receptors that bind signalling molecules released by neurons and convert these signals into intracellular changes influencing the behavior of cells. Neurotransmitter is used here in its most general sense, including not only messengers that act to regulate ion channels, but also those which act on second messenger systems and those which may act at a distance from their release sites. Included are receptors for neuromodulators, neuroregulators, neuromediators, and neurohumors, whether or not located at synapses.
Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified.
Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.
The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Thin layers of tissue which cover parts of the body, separate adjacent cavities, or connect adjacent structures.
Sites on an antigen that interact with specific antibodies.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
Biochemical identification of mutational changes in a nucleotide sequence.
The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
A family of transcription factors that share a unique DNA-binding domain. The name derives from viral oncogene-derived protein oncogene protein v-ets of the AVIAN ERYTHROBLASTOSIS VIRUS.
An essential amino acid. It is often added to animal feed.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Sequential operating programs and data which instruct the functioning of a digital computer.
Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.
Computer-based representation of physical systems and phenomena such as chemical processes.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
A genus of the Torpedinidae family consisting of several species. Members of this family have powerful electric organs and are commonly called electric rays.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Elements of limited time intervals, contributing to particular results or situations.
Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.
Protein modules with conserved ligand-binding surfaces which mediate specific interaction functions in SIGNAL TRANSDUCTION PATHWAYS and the specific BINDING SITES of their cognate protein LIGANDS.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Methods for determining interaction between PROTEINS.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.
Terbium. An element of the rare earth family of metals. It has the atomic symbol Tb, atomic number 65, and atomic weight 158.92.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Peptides composed of between two and twelve amino acids.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The region of DNA which borders the 5' end of a transcription unit and where a variety of regulatory sequences are located.
The process of cleaving a chemical compound by the addition of a molecule of water.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Proteins found in any species of fungus.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Tabular numerical representations of sequence motifs displaying their variability as likelihood values for each possible residue at each position in a sequence. Position-specific scoring matrices (PSSMs) are calculated from position frequency matrices.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Contractile tissue that produces movement in animals.
The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.
An essential amino acid that is required for the production of HISTAMINE.

Extra-vesicular binding of noradrenaline and guanethidine in the adrenergic neurones of the rat heart: a proposed site of action of adrenergic neurone blocking agents. (1/81452)

1 The binding and efflux characteristics of [14C]-guanethidine and [3H]-noradrenaline were studied in heart slices from rats which were pretreated with reserpine and nialamide. 2 Binding of both compounds occurred at extra-vesicular sites within the adrenergic neurone. After a brief period of rapid washout, the efflux of [14C]-guanethidine and [3H]-noradrenaline proceeded at a steady rate. The efflux of both compounds appeared to occur from a single intraneuronal compartment. 3 (+)-Amphetamine accelerated the efflux of [14C]-noradrenaline; this effect was inhibited by desipramine. 4 Unlabelled guanethidine and amantadine also increased the efflux of labelled compounds. Cocaine in high concentrations increased slightly the efflux of [14C]-guanethidine but not that of [3H]-noradrenaline. 5 Heart slices labelled with [3H]-noradrenaline became refractory to successive exposures to releasing agents although an appreciable amount of labelled compound was still present in in these slices. 6 It is suggested that [14C]-guanethidine and [3H]-noradrenaline are bound at a common extravesicular site within the adrenergic neurone. Binding of guanethidine to the extra-vesicular site may be relevant to its pharmacological action, i.e., the blockade of adrenergic transmission.  (+info)

The bioavailability, dispostion kinetics and dosage of sulphadimethoxine in dogs. (2/81452)

The disposition kinetics of sulphadimethoxine were studied in six normal beagle dogs after intravenous injection of a single dose (55 mg/kg). The median (range) distribution and elimination half times of the drug were 2.36 (2.06-3.35) hours and 13.10 (9.71-16.50) hours, respectively. Total body clearance of the drug had a median value of 21.7 ml/kg/h and a mean value of 21.4 ml/kg/h. While the overall tissue to plasma level ratio (k12/k21) of the drug was 0.55 after distribution equilibrium had been attained, analogue computer simulated curves showed that at 24 hours the fractions (percentage) of the dose in the central and tissue compartments were 12 and 11%, respectively. The drug was shown, by equilibrium dialysis method, to be highly bound to plasma proteins (greater than 75%) within the usual therapeutic range (50 to 150 mug/ml) of plasma levels. The systemic availability of sulphadimethoxine from the oral suspension was 32.8% (22.5-80.0). Since the absorption half time, 1.87 (0.86-3.22) hours, was considerably shorter than the half-life, 13.10 (9.71-16.50) hours, of the drug, the rate of absorption would have little influence on the dosage regimen. Based on the experimental data obtained, a satisfactory dosage regimen might consist of a priming dose of 55 mg/kg by the intravenous route and maintenance doses of either 27.5 mg/kg of sulphadimethoxine injection given intravenously or 55 mg/kg of the oral suspension administered at 24 hour intervals. The adequacy and duration of therapy will depend upon the clinical response obtained.  (+info)

Specific receptors for glucocorticoid in the cytoplasm of the liver of AH 130 tumor-bearing rats. (3/81452)

Specific receptors for dexamethasone (11beta, 17alpha, 21-trihydroxy-9alpha-fluoro-16alpha-methyl-1,4-pregnadiene-3,20-dione) in the cytoplasm of the liver from AH 130 (solid type) tumor-bearing rats markedly increased in the advanced stage of tumor growth. The cytoplasmic receptors of the livers of normal and tumor-bearing rats differed in their affinities for dexamethasone, and their apparent equilibrium (dissociation) constants (K) for dexamethasone were 4.0 and 2.6 X 10(-9) M, respectively. The rates of dissociation of dexamethasone-receptor complexes and the heat denaturations of the receptors in the livers of normal and tumor-bearing rats were similar. The glucocorticoid receptors of tumor-bearing rat liver had slightly higher affinities than did those of normal liver for all the steroids tested. Only a trace amount of receptors for dexamethasone could be detected in the cytoplasm of AH 130 ascites cells.  (+info)

The interaction of rhodium(II) carboxylates with enzymes. (4/81452)

The effect of rhodium(II) acetate, propionate, and methoxyacetate on the activity of 17 enzymes was evaluated. The enzymes were preincubated with the rhodium(II) complexes in order to detect irreversible inhibition. All enzymes that have essential sulfhydryl groups in or near their active site were found to be irreversibly inhibited. Those enzymes without essential sulfhydryl groups were not affected. In each case, the rate of inactivation closely paralleled the observed toxicity and antitumor activity of rhodium(II) carboxylates; that is, rhodium(II) propionate greater than rhodium(II) acetate greater than rhodium(II) methoxyacetate. In addition, those enzymes that have been demonstrated to be most sensitive to established sulfhydryl inhibitors, such as glyceraldehyde-3-phosphate dehydrogenase, were also most sensitive to rhodium(II) carboxylate inactivation. Proton nuclear magnetic resonance measurements made during the titration of rhodium(II) acetate with cysteine showed that breakdown of the carboxylate cage occurred as a result of reaction with this sulfhydryl-containing amino acid.  (+info)

The direct spectrophotometric observation of benzo(a)pyrene phenol formation by liver microsomes. (5/81452)

Optical spectral repetitive scan analysis during the oxidative metabolism of benzo(a)pyrene by liver microsomal suspensions reveals the time-dependent formation of an intermediate(s) of which the visible spectra resemble those of several benzo(a)pyrene phenols. Liver microsomes from 3-methylcholanthrene-treated rats showed a greater rate of formation of the phenols than did microsomes from control animals; the rate of formation catalyzed by liver microsomes from phenobarbital-pretreated rats was intermediate. When 3-hydroxybenzo(a)pyrene was used as a standard for comparison of activity, the rates of formation of phenols were compared when measured by fluorometric, spectrophotometric, or high-pressure liquid chromatographic analytical techniques. An epoxide hydrase inhibitor, 1,1,1-trichloropropene-2,3-oxide, enhanced phenol formation regardless of the source of liver microsomes, and 7,8-benzoflavone inhibited control and 3-methylcholanthrene-induced microsomal metabolism of benzo(a)pyrene, 7,8-Benzoflavone did not effect benzo(a)pyrene metabolism by liver microsomes from phenobarbital-pretreated rats. The effect of inhibitors on the spectrophotometric assay correlates well with the results obtained from benzo(a)pyrene metabolite analysis using high-pressure liquid chromatography.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (6/81452)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Action of partially thiolated polynucleotides on the DNA polymerase alpha from regenerating rat liver. (7/81452)

The effects of partially thiolated polynucleotides on the DNA polymerase alpha from regenerating rat liver were investigated. The enzyme was isolated from the nuclear fraction essentially according to the method of Baril et al.; it was characterized as the alpha polymerase on the basis of its response to synthetic templates and its inhibition with N-ethylmaleimide. Although polycytidylic acid had no effect on the DNA polymerase alpha either as a template or as an inhibitor, partially thiolated polycytidylic acid (MPC) was found to be a potent inhibitor, its activity being directly related to its extent of thiolation (percentage of 5-mercaptocytidylate units in the polymer). In comparison, the DNA polymerase beta which was purified from normal rat liver nuclear fraction, was much less sensitive to inhibition by MPC. Analysis of the inhibition of the alpha polymerase by the method of Lineweaver and Burk showed that the inhibitory action of MPC was competitively reversible with the DNA template, but the binding of the 7.2%-thiolated MPC to the enzyme was much stronger than that of the template (Ki/Km less than 0.03). Polyuridylic acid as such showed some inhibitory activity which increased on partial thiolation, but the 8.4%-thiolated polyuridylic acid was less active than the 7.2% MPC. When MPC was annealed with polyinosinic acid, it lost 80% of its inhibitory activity in the double-stranded configuration. However, 1 to 2%-thiolated DNA isolates were significantly more potent inhibitors than were comparable (1.2%-thiolated) MPC and showed competitive reversibility with the unmodified (but "activated") DNA template. These results indicate that the inhibitory activities of partially thiolated polynucleotides depend not only on the percentage of 5-mercapto groups but also on the configuration, base composition, and other specific structural properties.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (8/81452)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

Cryptic binding sites are the binding sites that are transiently formed in an apo form or that are induced by ligand binding. ... a binding site is a region on a macromolecule such as a protein that binds to another molecule with specificity. The binding ... Assessment of Binding Site Prediction Methods and a Protocol for Validation of Predicted Binding Sites". Cell Biochemistry and ... Binding of a ligand to a binding site on protein often triggers a change in conformation in the protein and results in altered ...
A primer binding site is a region of a nucleotide sequence where an RNA or DNA single-stranded primer binds to start ... The primer binding site is on one of the two complementary strands of a double-stranded nucleotide polymer, in the strand which ... retrieved 2021-11-30 Page for HIV primer binding site (PBS) at Rfam v t e (CS1 errors: missing periodical, Cis-regulatory RNA ... During PCR, two primers will bind to opposite template strands of DNA. The two primers point towards one another, allowing only ...
The PyrR binding site is an RNA element that is found upstream of a variety of genes involved in pyrimidine biosynthesis and ... Page for PyrR binding site at Rfam v t e (Cis-regulatory RNA elements, All stub articles, Molecular and cellular biology stubs) ... When the protein binds, a downstream terminator hairpin forms, repressing transcription of biosynthesis genes. Bonner ER, ... The RNA structure permits binding of PyrR protein which regulates pyrimidine biosynthesis in Bacillus subtilis. ...
... binding sites, it interacts with the bound nucleotide's phosphoryl groups. For the binding site to be able to bind a nucleotide ... An NTP binding site is a type of binding site found in nucleoside monophosphate (NMP) kinases, N can be adenosine or guanosine ... On the poliovirus RNA-dependent polymerase, also known as 3Dpol, there are two binding sites. Both binding sites contain lysine ... Nucleotide binding will cause conformational changes in the protein because the P-loop will bend. NTP binding sites play a role ...
A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA ... "Ribosomal Binding Site Sequence Requirements". www.thermofisher.com. Retrieved 2015-10-16. "Help:Ribosome Binding Site - parts. ... it is not considered a ribosome binding site. Eukaryotic ribosomes are known to bind to transcripts in a mechanism unlike the ... Alpha operon ribosome binding site Eukaryotic translation Bacterial translation Archaeal translation Gene prediction Shine, J ...
DNA binding sites are a type of binding site found in DNA where other molecules may bind. DNA binding sites are distinct from ... The sum of DNA binding sites of a specific transcription factor is referred to as its cistrome. DNA binding sites also ... It has been reported that some binding sites have potential to undergo fast evolutionary change. DNA binding sites can be ... Thus, we can distinguish between transcription factor-binding sites, restriction sites and recombination sites. Some authors ...
The AP-1 binding site, also known as the AP-1 promoter site, is a DNA sequence to which AP-1 transcription factors are able to ... The AP-1 binding site, in humans, has a nucleotide sequence of ATGAGTCAT, where A corresponds to adenine, T corresponds to ... "Differential functional significance of AP-1 binding sites in the promoter of the gene encoding mouse tissue inhibitor of ...
Page for Alpha operon ribosome binding site at Rfam v t e (Cis-regulatory RNA elements, All stub articles, Molecular and ... The alpha operon ribosome binding site in bacteria is surrounded by this complex pseudoknotted RNA structure. Translation of ... the mRNA produces 4 ribosomal protein products, one of which (S4) acts as a translational repressor by binding to the nested ...
The application of ChIP-seq methods has reliably discovered transcription factor binding sites and histone modification sites. ... Comprehensive List of transcription factor binding sites (TFBSs) databases based on ChIP-seq data as follows: Park, Peter J. (1 ... Ziebarth, Jesse D.; Bhattacharya, Anindya; Cui, Yan (1 January 2013). "CTCFBSDB 2.0: a database for CTCF-binding sites and ... expansion and enhancement of the collection of transcription factor binding sites models". Nucleic Acids Research. 44 (D1): ...
Page for Rous sarcoma virus (RSV) primer binding site (PBS) at Rfam v t e (Cis-regulatory RNA elements, All stub articles, ... This family represents a structured region around the Rous sarcoma virus (RSV) primer binding site (PBS). This region is known ...
... , also known as S2P endopeptidase or site-2 protease (S2P), is an enzyme (EC ... Membrane-bound transcription factor site-1 protease Brown MS, Goldstein JL (September 1999). "A proteolytic pathway that ... SREBP+site+2+protease at the US National Library of Medicine Medical Subject Headings (MeSH) S2P+endopeptidase at the US ... Known substrates include sterol regulatory element-binding protein (SREBP)-1, SREBP-2 and forms of the transcriptional ...
... , or site-1 protease (S1P) for short, also known as subtilisin/kexin-isozyme ... "Entrez Gene: Membrane-bound transcription factor peptidase, site 1". Brown MS, Goldstein JL (1999). "A proteolytic pathway that ... Membrane-bound transcription factor site-2 protease GRCh38: Ensembl release 89: ENSG00000140943 - Ensembl, May 2017 GRCm38: ... site-1+protease at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from the ...
"Revolutionary War Sites in South Bound Brook, New Jersey". Abraham Staats House "Abraham Staats House. Circa 1740". The ... Media related to Staats House (South Bound Brook, New Jersey) at Wikimedia Commons Official website Historic American Buildings ... At the Battle of Bound Brook, on April 13, 1777, private property was taken during the raid by British forces. Staats filed a ... Davis, T. E. (1895). "Appendix A". The Battle of Bound Brook. Washington Campground Association. pp. 25-26. Carter, George H., ...
Birmingham: Binding Site. ISBN 0-7044-2437-1. Slater NG, Cameron JS, Lessof MH (September 1976). "The Crithidia luciliae ... Serum is incubated with the beads and in the presence of anti-dsDNA antibodies, or any other ANA, the antibodies will bind and ... As a result of the highly specific nature of antibodies, they can be engineered to target and bind key motifs. These motifs can ... If anti-dsDNA antibodies are present, incubation of serum and the microarray allow for binding and the dots can then be ...
Pinkus LM (1977). "Glutamine binding sites". Methods in Enzymology. 46: 414-27. doi:10.1016/S0076-6879(77)46049-X. ISBN 978-0- ... Due to its similarity to glutamine it can enter catalytic centres of these enzymes and inhibits them by covalent binding, or ...
ファイアーエムブレム封印の剣 Q&A (in Japanese). Fire Emblem: The Binding Blade Official Website. Archived from the original on October 12, ... ファイアーエムブレムとは? (in Japanese). Fire Emblem: The Binding Blade Official Website. Archived from the original on March 8, 2016. ... The Binding Blade is set on the continent of Elibe, a setting shared with its prequel Fire Emblem. Elibe was engulfed a ... The Binding Blade is set on the fictional continent of Elibe, which has been dominated by humans for centuries following an ...
Mead, G. P., Stokes, R. P., Binding Site Limited. Birmingham: The Binding Site. ISBN 0-7044-8510-9. OCLC 41258931. "Acute ... Serum complement (C3 and C4) - Complement factors bind to antibodies to form immune complexes and a decreased serum complement ...
Birmingham: The Binding Site. ISBN 978-0704485105. Savige, J; Davies, D; Falk, RJ; Jennette, JC; Wiik, A (Mar 2000). " ...
"Transcription Factor Binding Sites". ElDorado. Genomatix. "SAPS". Biology Workbench. SDSU.[permanent dead link] "Large-scale ... CCDC82 has several predicted phosphorylation sites. There are 32 predicted serine phosphorylation sites, 5 threonine, and 3 ... Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific ...
... putative phosphoinositide binding site and putative peptide binding sites. The FAM43A gene has been identified in cDNA ... MicroRNA binding sites were identified and then compared to species conservation of FAM43A to determine likely 3' untranslated ... "FAM43A microRNA binding sites". Targetscan. Retrieved 21 May 2018. "FAM43A". PSORT II Prediction. Retrieved 8 April 2018. " ... Three phosphorylation sites were identified with conservation between human and mouse genotypes at T112-p, S114-p, and T-379-p ...
... including binding sites for zinc fingers and Kruppel-like transcription factors. The top 20 transcription binding sites as ... "C7orf43-promoter binding sites". Genomatix. Retrieved 5 April 2015. "Uncharacterized protein C7orf43 [Homo sapiens]". NCBI ... C7orf43 has three phosphorylated sites, Ser 517, Thr 541 and, Ser 546. All three sites are relatively well-conserved throughout ... There are several transcription factor binding sites located in this promoter, ...
Spiral Binding Corporate Site Spiral Binding Official webpage; (Articles needing additional references from July 2019, All ... Coil binding, also known as spiral binding, is a commonly used book binding style for documents. This binding style is known by ... Spiral coil binding spines are also available in more colors and sizes than other binding styles. Spiral Binding Company, ... Comb binding Wire binding Spiral Binding Plastic Coil 101 "New Jersey man celebrates his 105th birthday". News 12 New Jersey. ...
Each member of the ABCF subgroup consist of a pair of ATP binding domains. Six half transporters with ATP binding sites on the ... In this model, the substrate binding site alternates between outward- and inward-facing conformations. The relative binding ... prepared under suitable conditions contain inside-out oriented vesicles with the ATP binding site and substrate binding site of ... In the nucleotide binding site, the oxygen atoms of the β- and γ-phosphates of ATP are stabilized by residues in the Walker A ...
Upon binding cobalamin, important elements of the binding site appear to become structured, including an alpha-helix that forms ... In molecular biology, the vitamin B12-binding domain is a protein domain which binds to cobalamin (vitamin B12). It can bind ... Hanukoglu I (2015). "Proteopedia: Rossmann fold: A beta-alpha-beta fold at dinucleotide binding sites". Biochem Mol Biol Educ. ... When bound to the cobalamin-binding domain, the dimethylbenzimidazole ligand is replaced by the active histidine (His-on) of ...
Magnaghi-Jaulin L, Masutani H, Robin P, Lipinski M, Harel-Bellan A (1996). "SRE elements are binding sites for the fusion ... RNA-binding protein EWS is a protein that in humans is encoded by the EWSR1 gene on human chromosome 22, specifically 22q12.2. ... The normal EWS gene encodes an RNA binding protein closely related to FUS (gene) and TAF15, all of which have been associated ... The expression of a chimeric protein with the EWS transactivation domain fused to the DNA binding region of a transcription ...
RNA polymerase and H-NS DNA binding protein have overlapping binding sites; it is thought that H-NS regulates rRNA production ... DNA-binding domain DNA-binding protein DNA-binding protein from starved cells Transcription factor Drlica K, Rouviere-Yaniv J ( ... H-NS binds to DNA with an intrinsic curvature. In E. coli, H-NS binds to a P1 promoter decreasing rRNA production during ... It has been found that H-NS and RNA polymerase both bind to the P1 promoter and form a complex. When H-NS is bound with RNA ...
For example, many DNA binding proteins that have affinity for specific DNA binding sites bind DNA in only its double-helical ... Stormo GD (January 2000). "DNA binding sites: representation and discovery". Bioinformatics. 16 (1): 16-23. doi:10.1093/ ... 34 (Web Server issue): W369-73. doi:10.1093/nar/gkl198. PMC 1538909. PMID 16845028. Weirauch MT, Cote A, Norel R, Annala M, ... For example, an N-glycosylation site motif can be defined as Asn, followed by anything but Pro, followed by either Ser or Thr, ...
Apical oxygen binding sites are white. Talc crystal viewed along the [100] axis, looking along the layers of the crystal Talc ... Talc is a trioctahedral layered mineral; its structure is similar to pyrophyllite, but with magnesium in the octahedral sites ...
The FMN binding domain is homologous to flavodoxins, and the two domain fragment containing the FAD and NADPH binding sites is ... The oxygenase domain is a unique extended beta sheet cage with binding sites for heme and pterin. NOSs can be dimeric, ... Liu Q, Gross SS (1996). "Binding sites of nitric oxide synthases". Meth. Enzymol. Methods in Enzymology. 268: 311-24. doi: ... which is linked in the middle of the protein to a calmodulin-binding domain. Binding of calmodulin appears to act as a " ...
Stormo, G. D. (1 January 2000). "DNA binding sites: representation and discovery". Bioinformatics. 16 (1): 16-23. doi:10.1093/ ... The first use of PWMs was in the discovery of RNA sites that function as translation initiation sites. The advantages of PWMs ... algorithm to distinguish translational initiation sites in E. coli". Nucleic Acids Research. 10 (9): 2997-3011. doi:10.1093/nar ... work involves analysis of these interactions and developing pattern recognition algorithms to discover regulatory sites in DNA ...
2002). "Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase ... 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. ...
... bound into a book. In the first category were: Children, The Chorus Girl, A Play, Home, Misery, The Runaway, In Court, Vanka, ... Site also has translations of all the plays. Works by or about Anton Chekhov at Internet Archive Works by Anton Chekhov at ... Web. 3 November 2011. Letter to sister Masha, 28 June 1904. Letters of Anton Chekhov. "Anton Chekhov , Biography, Plays, Short ... Web. 3 November 2011. "Overview: 'The Lady with the Dog'." Characters in 20th-Century Literature. Laurie Lanzen Harris. Detroit ...
May 21: In a 4-1 victory over the Atlanta Braves, The Reds had 4 solo home runs in one game, including three in a row in the ... MLB.com Marty Brennaman puts his hair on the line Reds first to 80 wins after sweeping Diamondbacks MLB.com Playoff bound: Reds ... The Reds hit 10 home runs during the series. It was the first 4-game sweep of the Braves since 1990. At the end of the series, ... Both Leake and Cozart's home runs were caught by the same fan, Caleb Lloyd. Mike Leake went 8 innings with 6 strikeouts to earn ...
Official website (in Chinese) Official website (in English) Coordinates: 24°46′33″N 120°56′37″E / 24.7759°N 120.9437°E / ... "bound" form, and the other with its unbound and stative-verb form; they have slightly different ranges of denotations. As a ...
Web site for Sons and Daughters of Thunder Lane Debates - Resources. Oberlin College. Randy McNutt (September 28, 2003). Lane ... Lane Theological Seminary, bound by present day Gilbert, Chapel, Park, and Yale streets, continued to educate Presbyterian ... marks the site of the campus. The Lane Debates have been re-enacted in recent years by historians from Yale University, the ... Funded by the same Tappan brothers that had funded Oneida, his charge was "to find a site for a great national manual labor ...
Arrow declaration Cease and desist Copyright infringement Industrial espionage Inequitable conduct Non-binding opinion (United ... web site (United Kingdom patent law). ...
OPRTase, as a member of type I PRTases, has a prominent loop next to its active site. It is flexible in its open state and can ... The phosphoryl group binding entails juxtaposition between the carboxylate group and a negatively charged Asp residue (namely ... These two sites catalyze the last two steps of the de novo uridine monophosphate (UMP) biosynthetic pathway. After addition of ... For catalysis to occur, a dimer must exist in which a loop from one subunit covers the active site from the other one. In ...
Specialized websites exist that have a page on the legislation of tobacco products from various countries, allowing a ... it was not legally binding. When the Ministry of Health and Social Development (MoHSD) proposed tobacco legislation based on ... India Tobacco Control Laws website Tobacco Atlas Novotny, Thomas E.; Lum, Kristen; Smith, Elizabeth; Wang, Vivian; Barnes, ...
"Dwan Adelaide bound". Super Netball. 23 September 2021. "Smith makes move to Swifts". Super Netball. 23 September 2021. "Kadie- ... Official website (Use dmy dates from April 2022, Articles with short description, Short description matches Wikidata, All ... articles with unsourced statements, Articles with unsourced statements from December 2022, Official website not in Wikidata, ...
... targeting the integrin ligand binding site". Proc. Natl. Acad. Sci. U.S.A. 90 (21): 10003-7. Bibcode:1993PNAS...9010003B. doi: ... The N2 domain binds to the F pilus during virion infection freeing the N1 domain which then interacts with a TolA protein on ... Multiple cloning sites are sometimes used to ensure that the fragments are inserted in all three possible reading frames so ... Since the pIII is intact it does not matter whether the antigen remains bound to the phage. The issue of using Ff phages for ...
Official website Official website of the Valsad district council Valsad Collectorate Photographs of the district. (Articles ... It is bound by Navsari district to the north, Nashik district of Maharashtra state to the east, and Dadra and Nagar Haveli ... Bagwada is home to Arjungad Fort and several temples, including a Jain temple. Shri Bhav Bhaveshwar Mahadev Temple is located ...
Under canon law, Margaret was not bound by her first marriage contract as she was entered into the marriage before reaching the ... He also effectively imprisoned Margaret in her husband's home with the hope of preventing any further correspondence with her ... Her lavishly illuminated Book of Hours is open before her, with its protective cloth wrapper (called a "chemise" binding), ... "Margaret Beaufort". Westminster Abbey Official site. Retrieved 22 August 2013. Wyatt, Michael, The Italian Encounter with Tudor ...
... bind the COX site of E-cat. E-cat is regulated by E-allo in a way dependent on what ligand is bound to E-allo. Substrate and ... site of E-allo. Arachidonic acid can bind to E-cat and E-allo, but the affinity of AA for E-allo is 25 times that for Ecat. ... Heme binds only to the peroxidase site of E-cat while substrates, as well as certain inhibitors (e.g. celecoxib), ... Each monomer of the enzyme has a peroxidase and a PTGS (COX) active site. The PTGS (COX) enzymes catalyze the conversion of ...
"Fifth 'Pirates' Movie Sails to Top of Home Video Sales". Home Media Magazine. October 12, 2017. Archived from the original on ... Filming moved to Doug Jennings Park on the Spit from March 30 to June 15 for water-bound scenes. However, due to extreme sea ... The website's consensus reads, "Pirates of the Caribbean: Dead Men Tell No Tales proves that neither a change in directors nor ... Pirates of the Caribbean: Dead Men Tell No Tales was released on digital download by Walt Disney Studios Home Entertainment on ...
"U.S. Census website". United States Census Bureau. Retrieved 2008-01-31. "US Board on Geographic Names". United States ... Associates Lifespan Health Willoughby Drugs Advanced Plastics American Telephone Exchange Clifton Game Room Craft Book Binding ... Official website Coordinates: 35°22′58″N 87°59′31″W / 35.382777°N 87.99206°W / 35.382777; -87.99206 (Webarchive template ... William E. Smith, Jr., "T. S. Stribling: Southern Literary Maverick", University of North Alabama Collier Library website South ...
... starch in book bindings, manuscripts, glue, hair, flakes of dried skin, dead animals, plant materials, soiled clothing, and ... Cockroaches article American cockroach egg parasitoid on the UF/IFAS Featured Creatures website (Articles with short ...
... one-way southeast-bound) and Main Street (one-way northwest-bound). Just northwest of Fifth Street, Main and Church converge ... His home and practice was located in the house at 1005 Fifth Street (118-5318-0048). From 1959 until he retired, Dr. Kyle M. ... Their funeral home, established in 1868, is thought to be the oldest black business of the type in Lynchburg. Squire's son, ... His home and tennis court at 1422 Pierce Street (118-0225-0077) were individually listed in the National Register of Historic ...
A number of websites devoted to Modern Paganism and historical reenactment offer reference material and forums promoting the ... bare and bound; and two participles: present and past. The subjunctive has past and present forms. Finite verbs agree with ... Issued on microfiche and subsequently as a CD-ROM and on the World Wide Web.) Old English edition of Wikipedia, the free ... "When I got home, I ate dinner") don't use a wh-type conjunction, but rather a th-type correlative conjunction such as þā, ...
Other cars were pulled to the Bolton Station (site of the future Fifth Regiment Armory for the state militia on North Howard ... By the 1870s, most of the northeast-bound passenger traffic was being routed to a newer "Union Station" for several lines in ... Beyond on the horizon, is the grassy meadow hills of future Washington Hill, Broadway and the site of the Johns Hopkins ... were the site of the famous "Pratt Street Riot" on the next day, Friday, 19 April 1861 when transferring state militia troops ...
Compared to the binding laws enacted by states, the landscape of AI ethics principles paints a more diverse picture, with ... The region, contested and claimed by both India and Pakistan in its entirety and a site of an active armed conflict, witnessed ... The following functions are dominated by a handful of multinational companies: search engines (Google); web browsers (Google ... Opportunities and challenges for AI principles to address self-determination Non-binding AI principles suggested by actors ...
"The Official Website for Monkey High!". Viz Media. Retrieved November 5, 2017. Deracomiのお知らせ [Deracomi announcement]. Betsucomi ... Shogakukan later collected the individual chapters into eight bound volumes under the Flower Comics imprint. Viz Media licensed ...
Her home in Sydney was the 6,986-square-metre (75,200 sq ft), Elaine, on the waterfront at Seven Shillings Beach, Double Bay. ... was well known for his generous philanthropy and supported organisations such as the Boy Scouts and Outward Bound. In 1962, ... She was educated at by home by governesses, and also attended Sydney Church of England Girls' Grammar School. Ruth Dowling and ... Retrieved 25 September 2010.{{cite web}}: CS1 maint: unfit URL (link) Martha, Rutledge (1981). "Fairfax, Ruth Beatrice (1878- ...
... was a home-bound housewife. Messerli's brother later became a football coach and teacher, and his sister works for the Iowa ... Messerli grew up in a very ordinary American home. His father, a former coach, was the superintendent of the public schools, ... Green Integer website Jacket interview with Douglas Messerli (in conversation with Charles Bernstein) World Arts Review, ... Within this seemingly normal home life, Messerli developed at a young age a passion for theater, reading American and European ...
In January 2010 it was announced that the Guest And Chrimes site had been purchased and would be the new home of the club ... "Mark Robins looks Barnsley-bound as coaches join him on gardening leave". The Guardian. ISSN 0261-3077. Retrieved 1 October ... The final match of the 2005-06 season, home to MK Dons, was a winner-take-all relegation showdown. A scoreless draw, combined ... but safety was secured in the penultimate game of the season, a 2-1 home victory against Reading. Rotherham sold key players ...
The city is home to three publicly traded companies, and former Western Refining, now Marathon Petroleum, as well as home to ... In east El Paso, the north- and south-bound sections are known as Joe Battle Boulevard, or simply as "the Loop". South of I-10 ... Magoffin Home State Historic Site Plaza Hotel Union Depot El Paso High School University of Texas at El Paso The university's ... The 4,500-year-old site is one of the oldest villages in the United States. The wetlands are home to many birds, and over 200 ...
Official website (All articles with bare URLs for citations, Articles with bare URLs for citations from March 2022, Articles ... directly subordinate to the next-highest organizational body and instructions issued by the central FDJ leadership were binding ... brigade in their home area. In October 1947 the 'Berlin-FDJ' was legalised by the Allies. However, the 'West-FDJ' enjoyed ... Official website different in Wikidata and Wikipedia, Articles with ISNI identifiers, Articles with VIAF identifiers, Articles ...
... which contains antigen-presenting sites and carbohydrate-receptor binding regions. Groups 1, 2, 3, and 4 last shared a common ... 3 substitutions per site per year.[citation needed] The estimated mutation rate (1.21×10−2 to 1.41 ×10−2 substitutions per site ... "Mutations within the P2 domain of norovirus capsid affect binding to human histo-blood group antigens: evidence for a binding ... Tan M, Hegde RS, Jiang X (2004). "The P Domain of Norovirus Capsid Protein Forms Dimer and Binds to Histo-Blood Group Antigen ...
Schools portal Official website (Webarchive template wayback links, Articles with short description, Short description matches ... has a surrogate parent at the school and helps to inculcate the values and behavioral norms associated with the college-bound ... Official website different in Wikidata and Wikipedia, AC with 0 elements, Charter schools in New Orleans, Public high schools ...
Website of Sidian Bank Sidian Bank staff layoffs pointer to looming turmoil As of 24 October 2016. Banks portal Coordinates: 01 ... In June 2022, media reports indicated that Centum Investments had signed binding agreements to sale the 83.4 percent that it ...
Media related to World Food Prize at Wikimedia Commons Official website Iowa Hunger Summit official website Hall of Laureates ... However, the Foundation was bound by Alfred Nobel's will which did not allow for the creation of such a new prize. Borlaug ... The country is according to worldfoodprize.org, the official website of The World Food Prize Foundation. The 2013 award to ... Retrieved 3 December 2021.{{cite web}}: CS1 maint: others (link) Quinn, Kenneth M. (11 September 2012). "A Nobel Prize for Food ...
MapMan terms associated with a binding site. Binding site. Motif_534. Name. GBOXRELOSAMY3 ... Show species without binding site experiments. Click table-header(s) to enable sorting MapMan. Description. #Associated genes. ...
A new PII protein structure identifies the 2-oxoglutarate binding site. 1st May 2010 ...
... binding-site barrier phenomenon, can be seen in guinea pig micrometastases as small as 300 μm in diameter. Increasing the dose ... in part because of specific binding to the target antigen. Experiments presented here demonstrate an analogous phenomenon in ... Targeting Cancer Micrometastases with Monoclonal Antibodies: A Binding-Site Barrier *Saga, Tsuneo ... "binding-site barrier" phenomenon, can be seen in guinea pig micrometastases as small as 300 μm in diameter. Increasing the dose ...
Hydroxytryptamine Binding Site Development in Chick Embryo by Serotonergic Compounds J.Neurochem. 1985 44:544-551 ... 5-HT binding sites because the Hill coefficients were less than unity. When compared with the reported [3H]5-HT binding sites ( ... Saturable and specific binding sites for 5- [3H]hydroxytryptamine (5-HT, serotonin) characterized by a KD of 3.5-4.5 nM were ... However, the Hill coefficients for LSD and methysergide were less than unity which suggested that the [3H]5-HT binding sites in ...
Schetz, J.A.; Sibley, D.R. 2001: The binding-site crevice of the D4 dopamine receptor is coupled to three distinct sites of ... Schetz, J.A.; Sibley, D.R. 1999: The binding-site crevice of the D4 dopamine receptor is coupled to three unique sites of ... Mapping the binding-site crevice of the dopamine D-2 receptor. Javitch, J.A.; Fu, D.; Chen, J.; Karlin, A.. ... Javitch, J.A. 1997: Mapping the binding-site crevice of the D2 receptor Advances in Pharmacology 42: 412-415. Shi, L.; Javitch ...
Do chromatin loops and CTCF binding sites control hormone-regulated mammary-specific genetic islands?. Friday, September 18, ... we used ChIP-seq and identified five CTCF binding sites, three specific to mammary tissue and two shared with other cell types ... we employed CRISPR/CAS9 gene editing to eliminate individual CTCF binding sites. Structural consequences and their significance ... CCCTC-binding factor (CTCF) has been identified as a transcriptional regulator and a core architectural protein in establishing ...
Webinar: The Mysteries of Site-specific Binding Dynamics by Proteins. May 27, 2022 @ 3:00 pm - 4:00 pm EDT ...
We used bacterially expressed beta-galactosidase fusion proteins to localize the phospholipid binding domain of Acanthamoeba ... Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba myosin IC SK Doberstein, ... We confirm that the ATP-insensitive actin binding site is contained in the COOH-terminal 30 kD of the tail as previously shown ... SK Doberstein, TD Pollard; Localization and specificity of the phospholipid and actin binding sites on the tail of Acanthamoeba ...
I have already performed DEG analysis with EdgeR and selected the top 10 circRNAs (to start with) to find miRNA binding sites ... I am attempting to find miRNA binding sites for several circRNAs. ... Use of this site constitutes acceptance of our User Agreement and Privacy Policy. ... bind_rows(backSplicedJunctions, backSplicedJunctionsTool) 3. │ └─vctrs::vec_rbind(!!!dots, .names_to = .id) 4. └─vctrs::vec_ ...
One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a ... One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a ... One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a ... One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a ...
Wegmann, U., Horn, N., & Carding, S. R. (2013). Defining the bacteroides ribosomal binding site. Applied and Environmental ... Wegmann, U, Horn, N & Carding, SR 2013, Defining the bacteroides ribosomal binding site, Applied and Environmental ... Defining the bacteroides ribosomal binding site. In: Applied and Environmental Microbiology. 2013 ; Vol. 79, No. 6. pp. 1980-9. ... Defining the bacteroides ribosomal binding site. / Wegmann, Udo; Horn, Nikki; Carding, Simon R. ...
FAS1 domains, a family of cell adhesion molecules, have a carotenoid-binding function in an astaxanthin-binding protein AstaP ... A unique microalgal FAS1-containing astaxanthin (AXT)-binding protein (AstaP) binds a broad repertoire of carotenoids by a ... AXT-contacting AstaPo1 residues exhibit different conservation in AstaPs with the tentative carotenoid-binding function and in ... These structure-activity relationships provide the first step towards the sequence-based prediction of the carotenoid-binding ...
Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation. FEBS Letters. 2005 Aug ... Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation. / Millard, Christopher J ... Millard, CJ, Campbell, ID & Pickford, AR 2005, Gelatin binding to the 8F19F1 module pair of human fibronectin requires site- ... title = "Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation", ...
BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. The low-affinity guinea-pig ileum site ... BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. The low-affinity guinea-pig ileum site ... BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. The low-affinity guinea-pig ileum site ... BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. The low-affinity guinea-pig ileum site ...
A calcium atom binds at the bottom of the funnel-shaped tunnel located in the centre of the propeller. Two ligand-binding sites ... A calcium atom binds at the bottom of the funnel-shaped tunnel located in the centre of the propeller. Two ligand-binding sites ... as well as the conserved water molecules in all of the sites. Both sites can bind the two anomers, [alpha] and [beta], of N- ... as well as the conserved water molecules in all of the sites. Both sites can bind the two anomers, [alpha] and [beta], of N- ...
Posts about Tistriallal Binds written by Shaun Prescott ... Crawlspace: A Music Website From Australia. Tag Archives: ... Tistriallal Binds Reviews. Grog Pappy - Ferret Compilation (c30). April 15, 2013. Shaun PrescottGrog Pappy, Herby Cock-Hands, ... But together with Herby Cock-Hands, Tistriallal Binds, Samaan Fleck and The Mermaids, this whole mess of otherwise rotely ... Moffarfarrah, Newcastle, Prehistoric Fucken Moron, Rats With Wings, Samaan Fleck, The Mermaids, Tistriallal Binds Leave a ...
... a feature that correlates with distinct binding activities on its ATF site. We define five ATF site-binding activities in F9 ... binding site, and consistent with this we show that complexes formed on this site are regulated as F9 EC cells differentiate. ... Another ATF site-containing promoter is that of the human vasoactive intestinal polypeptide (VIP). In contrast to the E4 ... From these activities, we define those that bind in a promoter-specific or promoter-common fashion to the E4 and VIP promoters ...
Corrigendum to Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site: Molecular and behavioural ... Corrigendum to Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site: Molecular and behavioural ... Corrigendum to Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site : Molecular and behavioural ... title = "Corrigendum to Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site: Molecular and ...
Direct synthesis and characterization of site-specific deoxyguanosyl and deoxyadenosyl adducts derived from the binding of PAH ... Direct synthesis and characterization of site-specific deoxyguanosyl and deoxyadenosyl adducts derived from the binding of PAH ... Direct synthesis and characterization of site-specific deoxyguanosyl and deoxyadenosyl adducts derived from the binding of PAH ... Direct synthesis and characterization of site-specific deoxyguanosyl and deoxyadenosyl adducts derived from the binding of PAH ...
These targets were identified based on the presence of predicted regulator binding sites or experimental regulator binding in ... Transcriptional regulation information for a gene, including any predicted DNA binding site motifs (YeTFaSCo) for the genes ... based primarily on experiments showing that a regulator binds to the genes promoter or affects the genes transcription when ... DNA Binding Site Motifs Binding sites motifs as predicted by YeTFaSCo. Targets This table lists putative transcriptional ...
... (1651 views). Sandomenico A, Monti ... Ige-Binding Properties And Selectivity Of Peptide Mimics Of The Fcvarepsilonri Binding Site. Filter those results▼ ... Ige-Binding Properties And Selectivity Of Peptide Mimics Of The Fcvarepsilonri Binding Site. Fc epsilon RI alpha found on the ... Ige-Binding Properties And Selectivity Of Peptide Mimics Of The Fcvarepsilonri Binding Site. No results. ...
Your insurance website visitors can now purchase an Empower policy through the binding online technology of TurboRater for ... ITC Joins Agents Council for TechnologyITC Adds Empower to Binding Online with TurboRater for Websites ... ITC is actively working to add more insurance carriers to the bind online feature of TurboRater for Websites.. About ITC. ... ITC Releases Binding Online with TurboRater for WebsitesITC Releases Alliance United to TurboRater in California ...
... FELICE B;CATTOGLIO C; ... We report a bioinformatic analysis of the distribution of transcription factor binding sites (TFBSs) flanking >4,000 ... We report a bioinformatic analysis of the distribution of transcription factor binding sites (TFBSs) flanking ,4,000 integrated ... This study identifies TFBSs as differential genomic determinants of retroviral target site selection in the human genome, and ...
Cookies on this website We use cookies to ensure that we give you the best experience on our website. If you click Accept all ... Interaction of the Glycine Receptor Alpha 1 Binding Site with Partial Agonists ... Interaction of the Glycine Receptor Alpha 1 Binding Site with Partial Agonists ...
Same Day Managed IT Service Company San Antonio Site Same Day Managed IT Service Company San Antonio Website Same Day Managed ... Same Day Managed IT Services for Business San Antonio Site Same Day Managed IT Services for Business San Antonio Website Same ... San Antonio Same Day Managed IT Service Company Site San Antonio Same Day Managed IT Service Company Website San Antonio Same ... San Antonio Same Day Managed IT Services for Business Site San Antonio Same Day Managed IT Services for Business Website San ...
To determine whether the 3614-3643 region is a regulatory site/interaction domain … ... The calmodulin C lobe binding region (residues 3614-3643) on the sarcoplasmic reticulum Ca2+ release channel (RyR1) is thought ... A calmodulin binding domain of RyR increases activation of spontaneous Ca2+ sparks in frog skeletal muscle J Biol Chem. 2005 ... Our data support a model in which the calmodulin binding domain of RyR1 modulates channel activity by at least two mechanisms: ...
Ribosome Binding Site Characterization. A ribosomal binding site sequence is a specific mRNA sequence that folds in such a way ... A perfect way to implement this variation in the alkane degradation pathway is by using ribosome binding sites with varying ... This ribosome binds to the mRNA molecule and starts translation of the mRNA into protein. ...
We probe its binding site and mechanism of action by computational analysis based on our recently reported KCNQ1 and KCNQ1/ ... Probing Binding Sites and Mechanisms of Action of an I-Ks Activator by Computations and Experiments. ... Results from a pocket analysis and docking exercise suggest that ML277 binds to a side pocket in KCNQ1 and the KCNE1-free side ... Second, ML277 binding induces global motions in the channel, including regions critical for KCNQ1 gating transitions. We ...
Its sialic acid-binding site is located within the NH2-terminal (membrane-distal) V-set domain. Here we have carried out site- ... A CD8 alpha-based molecular model predicts that these residues form a contiguous binding site on the GFCCC beta-sheet of the ... A subset of nonconservative mutations disrupted sialic acid-dependent binding without affecting binding of three monoclonal ... directed mutagenesis in an attempt to identify the binding site of sialoadhesin. ...
Were only bound to me. (She was) She was only bound (She was only bound). (Ah, ah) Only bound to me (She was only bound to me) ... Ah, ah) Only bound to me (She was only bound to me). (She was) She was only bound (She was only bound). (Ah, ah) Only bound to ... She was) She was only bound (Only bound). (She was only bound) Only bound to me (To me). (She was) She was only bound… (Only ... She was only bound. Only bound to me. I was always around. Crawling around on my knees ...
  • Covalent surface immobilization of proteins for binding assays is typically performed non-specifically via lysine residues. (uni-muenchen.de)
  • All of these enzymes can be used to site-specifically and covalently ligate proteins of interest via short recognition sequences. (uni-muenchen.de)
  • We used bacterially expressed beta-galactosidase fusion proteins to localize the phospholipid binding domain of Acanthamoeba myosin IC to the region between amino acids 701 and 888 in the NH2-terminal half of the tail. (rupress.org)
  • AXT-contacting AstaPo1 residues exhibit different conservation in AstaPs with the tentative carotenoid-binding function and in FAS1 proteins generally, which supports the idea of AstaP neofunctionalization within green algae. (nature.com)
  • Most of the circulating testosterone is bound to carrier proteins (SHBG = sex hormone-binding globulin). (cdc.gov)
  • Following influenza virus infection or receipt of a flu vaccine, the body's immune system develops antibodies that recognize and bind to "antigenic sites," which are regions found on an influenza virus' surface proteins. (cdc.gov)
  • The HI test works by measuring how well antibodies bind to the HA proteins and prevent them from "gluing" red blood cells together (i.e., hemagglutination inhibition). (cdc.gov)
  • Such elements provide sites for specialized proteins (called transcription factors) to attach (bind) and either activate or repress the process by which the information from genes is turned into proteins (transcription). (medlineplus.gov)
  • Enhancers provide binding sites for proteins that help activate transcription. (medlineplus.gov)
  • Silencers provide binding sites for proteins that repress transcription. (medlineplus.gov)
  • Insulators provide binding sites for proteins that control transcription in a number of ways. (medlineplus.gov)
  • Proteasomes, cal systems, including post-infectious pro- which generate peptides from cytoplasmic tective immune responses, only some pep- proteins for the class I pathway, seem to tides from a large number of potential candi- have preferred cleavage sites flanking domi- dates are actually the target of a vigorous nant CD8+ T cell epitopes in protein se- immune response. (who.int)
  • A large number of fac- phagolysosomal compartment and are in- tors influence dominance and crypticity of volved in proteolytic processing of endocy- peptide epitopes, basically availability for tosed proteins in the MHC class II pathway, MHC binding, MHC binding itself, and the display preferential cleavage of dibasic (RR, recognition of the MHC:peptide complex by KK, KR or RK) sites (6). (who.int)
  • The NF-kB and NF-IL-6 DNA binding proteins were immunochemically characterized as a heterodimer p65/p50 and a homodimer C/EBP beta, respectively. (cdc.gov)
  • However, receptors that either have lysines near their binding pockets, or whose presence at the sensor surface is electrostatically disfavoured, can be hard to probe. (uni-muenchen.de)
  • High specific activity [ 3 H]BK and an enzyme inhibitor 'cocktail' has enabled us to label two BK binding sites with different affinity and peptide specificity in several guinea-pig tissues. (johnshopkins.edu)
  • Buku, A., Mendlowitz, M., Condie, B.A., Price, J.A., Histamine-releasing activity and binding to the FcepsilonRI alpha human mast cell receptor subunit of mast cell degranulating peptide analogues with alanine substitutions (2003) J. Med. (cnr.it)
  • One of these specific binding domains, which itself contains at least two distinct receptor binding sites, resides within a fragment of viral DNA that maps 110-449 bp upstream of the promoter for MTV RNA synthesis. (houstonmethodist.org)
  • Thus, it appears that this assay will be useful for assessing the biological significance of the receptor binding sites detected in vitro. (houstonmethodist.org)
  • We successfully employed this enzymatic surface functionalization approach to study the binding kinetics of two different receptor-ligand pairs. (uni-muenchen.de)
  • Saturation and displacement studies using unlabeled 5-HT as the displacing ligand suggested a single population of binding sites. (erowid.org)
  • Using a novel immobilized ligand lipid binding assay, we determined that myosin I can bind to several different acidic phospholipids, and that binding requires a minimum of 5 mol% acidic phospholipid in a neutral lipid background. (rupress.org)
  • Fasciclins (FAS1) are ancient adhesion protein domains with no common small ligand binding reported. (nature.com)
  • Two ligand-binding sites, [alpha] and [beta], are present in each of the two protomers in the dimer. (univr.it)
  • Monoclonal antibodies penetrate bulky tumors poorly after intravenous administration, in part because of specific binding to the target antigen. (harvard.edu)
  • The human TAP (transporter natural immune response is generated upon associated with antigen presentation) mole- immunization with the whole protein are cule selects peptides according to a binding called dominant epitopes. (who.int)
  • The direct synthesis method is a relatively simple approach for generating modified oligonucleotides of defined base sequence with site-specifically placed polycyclic aromatic hydrocarbon diol epoxide-modified deoxyguanosyl and deoxyadenosyl residues. (umn.edu)
  • Smaller amounts of modified oligonucleotides containing a single modified guanosyl residue in oligonucleotides 10-11 bases long containing up to three other unmodified guanine residues can be readily generated in smaller quantities for site-directed mutagenesis and other studies. (umn.edu)
  • Further studies using the yeast two-hybrid system revealed that the NS5 region (residues 320-368) immediately adjacent to the NLS contained an importin - binding site that abuts or overlaps the binding site for the NS3 protein (protease/helicase). (who.int)
  • various serotonergic compounds were injected into the chorioallantoic fluid of the eggs at different times during embryonic development, Multiple pretreatments with d,l-5-hydroxytryptophan, 5-HT, or BOL were found to have no significant effects on either the affinity (KD) or number (BmaX) of specific [3H]5-HT binding sites. (erowid.org)
  • Chemical shift differences between the glycoforms have revealed an intimate interaction between one N-linked sugar and the polypeptide that is critical for gelatin binding, as shown by affinity chromatography. (port.ac.uk)
  • In the guinea-pig ileum the high-affinity site has an equilibrium dissociation constant (K(d)) for [ 3 H]BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. (johnshopkins.edu)
  • The low-affinity guinea-pig ileum site displays a K(d) of 910 pM, a maximum number of bindig sites of 14 pmol/g of tissue wet weight and shows a greater selectivity for BK analogs over Lysyl-BK analogs. (johnshopkins.edu)
  • The potencies of a series of BK analogs at the high-affinity guinea-pig ileum site correlate well with their potencies in contracting ileal smooth muscle. (johnshopkins.edu)
  • In the guinea-pig ileum the high-affinity site has an equilibrium dissociation constant (K(d)) for [3H]BK of 13 pM and a maximal number of binding sites of 8.3 pmol/g of tissue wet weight. (johnshopkins.edu)
  • The binding affinity in the blood to the heme which normally carries oxygen reduces the available sites for oxygen. (cdc.gov)
  • Transcriptional regulation information for a gene, including any predicted DNA binding site motifs ( YeTFaSCo ) for the gene's protein product, as well as any of its targets (genes it regulates) or regulators (genes that regulate it), based on experimental evidence. (yeastgenome.org)
  • We show that gamma-retroviral, but not lentiviral vectors, integrate in genomic regions enriched in cell-type specific subsets of TFBSs, independently from their relative position with respect to genes and transcription start sites. (unisr.it)
  • Molecular modeling indicates that hopeahainol A binds at the entrance of the long but narrow AChE active site gorge because it is too bulky to be accommodated within the gorge without severe distortion of the gorge as depicted in AChE crystal structures. (elsevier.com)
  • Realizing Abundant Chirality Inversion of Supramolecular Nanohelices by Multiply Manipulating the Binding Sites in Molecular Blocks. (bvsalud.org)
  • However, mimicking this biological phenomenon and understanding at a molecular level its mechanism with the multiple binding sites by establishing an artificial system still remains a challenge. (bvsalud.org)
  • Corrigendum to Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site: Molecular and behavioural aspects [Eur. (bgu.ac.il)
  • We hypothesize that bound hopeahainol A induces conformational changes in the AChE active site that allow binding of additional hopeahainol A molecules, a phenomenon that would be unprecedented for a reversible inhibitor that apparently forms no covalent bonds with AChE. (elsevier.com)
  • The amino acids that participate in the contacts have been identified, as well as the conserved water molecules in all of the sites. (univr.it)
  • Natural product inhibitors of AChE are of interest both because they offer promise as inexpensive drugs for symptomatic relief in Alzheimer's disease and because they may provide insights into the structural features of the AChE catalytic site. (elsevier.com)
  • We have conducted a site-specific analysis of the structural and functional consequences of N-linked glycosylation in the 8 F1 9 F1 module pair. (port.ac.uk)
  • Three other binding domains lie downstream of the promoter and within the MTV primary transcription unit. (houstonmethodist.org)
  • Another ATF site-containing promoter is that of the human vasoactive intestinal polypeptide (VIP). (ox.ac.uk)
  • In contrast to the E4 promoter, the VIP promoter is transcriptionally inactive throughout differentiation, a feature that correlates with distinct binding activities on its ATF site. (ox.ac.uk)
  • From these activities, we define those that bind in a promoter-specific or promoter-common fashion to the E4 and VIP promoters. (ox.ac.uk)
  • These targets were identified based on the presence of predicted regulator binding sites or experimental regulator binding in the target promoter, and/or changes in the target gene's transcript levels in regulator mutant strains. (yeastgenome.org)
  • IL-6 induction paralleled increased DNA binding activity to the NF-kB and NF-IL-6 recognized sites in the IL-6 promoter. (cdc.gov)
  • Asbestos and H2O2-induced DNA binding activity to the NF-kB and NF-IL-6 binding sites of the IL-6 promoter were inhibited by antioxidants. (cdc.gov)
  • We have attempted to sort out these effects by using the binding interaction of [ 3 H]BK at the membrane levels with in vitro receptor binding techniques. (johnshopkins.edu)
  • Do chromatin loops and CTCF binding sites control hormone-regulated mammary-specific genetic islands? (nih.gov)
  • In a quest to understand the significance of an ordered, and possibly cell-specific, chromatin structures in the regulation of the casein island, we used ChIP-seq and identified five CTCF binding sites, three specific to mammary tissue and two shared with other cell types. (nih.gov)
  • To understand their functions in regulating the mammary-specific and the juxtaposed salivary-specific loci, we employed CRISPR/CAS9 gene editing to eliminate individual CTCF binding sites. (nih.gov)
  • poor antibody penetration, attributable to a "binding-site barrier" phenomenon, can be seen in guinea pig micrometastases as small as 300 μm in diameter. (harvard.edu)
  • Endogenous testosterone released from the sample by ANS (8-anilino-1-naphthalene sulfonic acid) and norgestrel competes with the added testosterone derivative labeled with ruthenium complex for the binding sites on the biotinylated antibody. (cdc.gov)
  • The binding sites of the labeled antibody become occupied partly by the sample analyte (depending on its concentration) and partly by the ruthenium-labeled hapten to form the respective immunocomplexes. (cdc.gov)
  • Furthermore, we report the identification of the 3' end of the 16S rRNA of Bacteroides ovatus and analyze in detail its ribosomal binding site, thus defining a core region necessary for efficient translation, which we have incorporated into the design of our expression vectors. (uea.ac.uk)
  • CCCTC-binding factor (CTCF) has been identified as a transcriptional regulator and a core architectural protein in establishing chromatin loops. (nih.gov)
  • To study [3H]5-HT-'binding site regulation and development. (erowid.org)
  • The overall evidence indicated that the chick embryo htnin may have a functioning serotonergic system and that the chick embryo may be an ideal system for the study of [3H]5-HT binding site regulation and development. (erowid.org)
  • The induction of diverse chirality regulation in nature by multiple binding sites of biomolecules is ubiquitous and plays an essential role in determining the biofunction of biosystems. (bvsalud.org)
  • In addition, synergistically manipulating the carboxy and amide binding sites from a single LPPF molecule to simultaneously interact with different naphthalene derivatives leads to chirality regulation . (bvsalud.org)
  • The sequences required for this in vivo regulation include the activating transcription factor (ATF)-binding site, and consistent with this we show that complexes formed on this site are regulated as F9 EC cells differentiate. (ox.ac.uk)
  • We define five ATF site-binding activities in F9 cells that can be distinguished from each other by their precise sequence requirements and their regulation during differentiation. (ox.ac.uk)
  • The central event in the cel- mediated responses) or in both the afferent lular immune response to invading microor- and effector limbs of the immune response ganisms is the specific recognition of for- (T cell-mediated responses - e.g., delayed eign peptides bound to major histocompat- hypersensitivity). (who.int)
  • We report a bioinformatic analysis of the distribution of transcription factor binding sites (TFBSs) flanking >4,000 integrated proviruses in human hematopoietic and non-hematopoietic cells. (unisr.it)
  • This study identifies TFBSs as differential genomic determinants of retroviral target site selection in the human genome, and suggests that transcription factors binding the LTR enhancer may synergize with the integrase in tethering retroviral pre-integration complexes to transcriptionally active regulatory regions. (unisr.it)
  • Promoters provide binding sites for the protein machinery that carries out transcription. (medlineplus.gov)
  • We conducted inhibitor competition experiments in which AChE inhibition was measured with hopeahainol A together with either edrophonium (which binds at the base of the gorge) or thioflavin T (which binds to the peripheral or P-site near the gorge mouth). (elsevier.com)
  • Using two independent assays, we find that purified rat liver glucocorticoid receptor protein binds specifically to at least four widely separated regions on pure MTV proviral DNA. (houstonmethodist.org)
  • Analysis of sequences flanking the integration sites of Moloney leukemia virus (MLV)- and human immunodeficiency virus (HIV)-derived vectors carrying mutations in their long terminal repeats (LTRs), and of HIV vectors packaged with an MLV integrase, indicates that the MLV integrase and LTR enhancer are the viral determinants of the selection of TFBS-rich regions in the genome. (unisr.it)
  • A new PII protein structure identifies the 2-oxoglutarate binding site. (jic.ac.uk)
  • A unique microalgal FAS1-containing astaxanthin (AXT)-binding protein (AstaP) binds a broad repertoire of carotenoids by a largely unknown mechanism. (nature.com)
  • We conclude that the association of the myosin IC tail with acidic phospholipid head groups supplies much of the energy for binding myosin I to biological membranes, but probably not specificity for targeting myosin I isoforms to different cellular locations. (rupress.org)
  • These structure-activity relationships provide the first step towards the sequence-based prediction of the carotenoid-binding FAS1 members. (nature.com)
  • The inhibition at higher hopeahainol A concentrations was completely reversed on dilution and blocked by bound edrophonium. (elsevier.com)
  • The binding of [ 3 H]BK in the guinea-pig ileum is inhibited by physiological concentrations of monovalent and divalent cations. (johnshopkins.edu)
  • The binding of [3H]BK in the guinea-pig ileum is inhibited by physiological concentrations of monovalent and divalent cations. (johnshopkins.edu)
  • To address this, concentrations of testosterone, sex hormone binding globulin, androstanediol glucuronide (a metabolite of dihydrotestosterone) and estradiol were measured in stored serum specimens from men examined in the morning sample of the first phase of NHANES III (1988-1991). (cdc.gov)
  • When they visit an insurance agency website with TurboRater for Websites on it, online insurance shoppers can get auto insurance quotes from multiple insurance companies. (turborater.com)
  • If necessary, you can also use the Manual binding configuration for the multiple instances of ECT components explained in the next section. (who.int)
  • Intriguingly, a cyanobacterial homolog with a similar domain structure cannot bind carotenoids under identical conditions. (nature.com)
  • The gelatin (denatured collagen) binding domain of the extracellular matrix protein fibronectin contains three potential N-glycosylation sites. (port.ac.uk)
  • In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. (nature.com)
  • Manual binding is recommended when ECT is used in a JavaScript framework. (who.int)
  • Saturable and specific binding sites for 5- [3H]hydroxytryptamine (5-HT, serotonin) characterized by a KD of 3.5-4.5 nM were detected in the chick embryo brain and were shown to develop linearly as a function of age, weight, and protein content. (erowid.org)
  • Millard, CJ, Campbell, ID & Pickford, AR 2005, ' Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation ', FEBS Letters , vol. 579, no. 20, pp. 4529-4534. (port.ac.uk)
  • The instant binding online technology then gives those consumers the option to purchase a policy from one of the available carriers directly on the agent's website. (turborater.com)
  • The importin -binding site has also been shown to be a functional NLS (bNLS). (who.int)
  • The information on this site should not be used as a substitute for professional medical care or advice. (medlineplus.gov)
  • However, the Hill coefficients for LSD and methysergide were less than unity which suggested that the [3H]5-HT binding sites in the chick embryo brain may be more similar to those found in rat spinal cord than rat forebrain. (erowid.org)
  • Two similar sites can also be discriminated in kidney and heart. (johnshopkins.edu)
  • HI test results can tell us whether antibodies developed after vaccination (or infection) with one virus can recognize and bind to other viruses, which means these other viruses are similar to the vaccine virus. (cdc.gov)
  • The amide and hydroxy group of naphthalene derivatives prefer to bind with the carboxy group of LPPF, while carboxylic groups and fluoride atoms tend to bind with the amide moiety of LPPF. (bvsalud.org)
  • I have already performed DEG analysis with EdgeR and selected the top 10 circRNAs (to start with) to find miRNA binding sites using circRNAprofiler. (bioconductor.org)
  • In order to make an ECT component work, when the DOM of your web application is fully loaded, the ECT library searches in your HTML the proper elements added for the Embedded Coding Tool (or the Embedded Browser) and binds it with the ECT component. (who.int)
  • We confirm that the ATP-insensitive actin binding site is contained in the COOH-terminal 30 kD of the tail as previously shown for Acanthamoeba myosin IA. (rupress.org)

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