Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Dictionaries, ChemicalEpitopes: Sites on an antigen that interact with specific antibodies.DictionaryProtein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Receptors, Chemokine: Cell surface glycoproteins that bind to chemokines and thus mediate the migration of pro-inflammatory molecules. The receptors are members of the seven-transmembrane G protein-coupled receptor family. Like the CHEMOKINES themselves, the receptors can be divided into at least three structural branches: CR, CCR, and CXCR, according to variations in a shared cysteine motif.Receptors, CXCR4: CXCR receptors with specificity for CXCL12 CHEMOKINE. The receptors may play a role in HEMATOPOIESIS regulation and can also function as coreceptors for the HUMAN IMMUNODEFICIENCY VIRUS.Chemokine CXCL12: A CXC chemokine that is chemotactic for T-LYMPHOCYTES and MONOCYTES. It has specificity for CXCR4 RECEPTORS. Two isoforms of CXCL12 are produced by alternative mRNA splicing.Chemokine CXCL10: A CXC chemokine that is induced by GAMMA-INTERFERON and is chemotactic for MONOCYTES and T-LYMPHOCYTES. It has specificity for the CXCR3 RECEPTOR.Chemokine CCL5: A CC-type chemokine that is a chemoattractant for EOSINOPHILS; MONOCYTES; and LYMPHOCYTES. It is a potent and selective eosinophil chemotaxin that is stored in and released from PLATELETS and activated T-LYMPHOCYTES. Chemokine CCL5 is specific for CCR1 RECEPTORS; CCR3 RECEPTORS; and CCR5 RECEPTORS. The acronym RANTES refers to Regulated on Activation, Normal T Expressed and Secreted.Endocrinology: A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.Internal Medicine: A medical specialty concerned with the diagnosis and treatment of diseases of the internal organ systems of adults.Societies, Medical: Societies whose membership is limited to physicians.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.BooksPeriodicals as Topic: A publication issued at stated, more or less regular, intervals.Contracts: Agreements between two or more parties, especially those that are written and enforceable by law (American Heritage Dictionary of the English Language, 4th ed). It is sometimes used to characterize the nature of the professional-patient relationship.Contract Services: Outside services provided to an institution under a formal financial agreement.Salaries and Fringe Benefits: The remuneration paid or benefits granted to an employee.Occupational Diseases: Diseases caused by factors involved in one's employment.Occupational Exposure: The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.Employment: The state of being engaged in an activity or service for wages or salary.United StatesSerum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Histology: The study of the structure of various TISSUES of organisms on a microscopic level.Phenolsulfonphthalein: Red dye, pH indicator, and diagnostic aid for determination of renal function. It is used also for studies of the gastrointestinal and other systems.Dicarboxylic AcidsSequence Tagged Sites: Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.Bilirubin: A bile pigment that is a degradation product of HEME.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Dogfish: Sharks of the family Squalidae, also called dogfish sharks. They comprise at least eight genera and 44 species. Their LIVER is valued for its oil and its flesh is often made into fertilizer.Elasmobranchii: A subclass of cartilaginous fish comprising the SHARKS; rays; skates (SKATES (FISH);), and sawfish. Elasmobranchs are typically predaceous, relying more on smell (the olfactory capsules are relatively large) than sight (the eyes are relatively small) for obtaining their food.Diazepam Binding Inhibitor: An 86-amino acid polypeptide, found in central and peripheral tissues, that displaces diazepam from the benzodiazepine recognition site on the gamma-aminobutyric acid receptor (RECEPTORS, GABA). It also binds medium- and long-chain acyl-CoA esters and serves as an acyl-CoA transporter. This peptide regulates lipid metabolism.Angiotensins: Oligopeptides which are important in the regulation of blood pressure (VASOCONSTRICTION) and fluid homeostasis via the RENIN-ANGIOTENSIN SYSTEM. These include angiotensins derived naturally from precursor ANGIOTENSINOGEN, and those synthesized.Captopril: A potent and specific inhibitor of PEPTIDYL-DIPEPTIDASE A. It blocks the conversion of ANGIOTENSIN I to ANGIOTENSIN II, a vasoconstrictor and important regulator of arterial blood pressure. Captopril acts to suppress the RENIN-ANGIOTENSIN SYSTEM and inhibits pressure responses to exogenous angiotensin.Skates (Fish): The common name for all members of the Rajidae family. Skates and rays are members of the same order (Rajiformes). Skates have weak electric organs.Sharks: A group of elongate elasmobranchs. Sharks are mostly marine fish, with certain species large and voracious.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Receptors, G-Protein-Coupled: The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.Ligands: A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Radioligand Assay: Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).Kinetics: The rate dynamics in chemical or physical systems.Pharmacology: The study of the origin, nature, properties, and actions of drugs and their effects on living organisms.Electrolytes: Substances that dissociate into two or more ions, to some extent, in water. Solutions of electrolytes thus conduct an electric current and can be decomposed by it (ELECTROLYSIS). (Grant & Hackh's Chemical Dictionary, 5th ed)Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Membrane Potential, Mitochondrial: The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.Polymers: Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Nanoparticles: Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.Depth Perception: Perception of three-dimensionality.

Blood thymidine level and iododeoxyuridine incorporation and reutilization in DNA in mice given long-acting thymidine pellets. (1/13926)

A long-acting thymidine pellet consisting of 190 mg of cholesterol and 60 mg of thymidine has been developed for the study of thymidine metabolism and reutilization in vivo. Implantation of such a pellet s.c. in adult mice will maintain the blood plasma concentration of thymidine at levels between 40 and 8 X 10(-6) M, which are from 36 to 7 times those of normal mice, for periods up to 48 hr. During this period, in vivo uptake and reutilization of [125I]iododeoxyuridine, a thymidine analog, into intestinal and tumor DNA were almost completely suppressed. While iododeoxyuridine reutilization is not large in normal proliferative tissue even in the absence of pellet implants, reutilization of over 30% was measured in large, rapidly growing ascites tumors. The inhibition of iododeoxyuridine incorporation by elevated thymidine blood levels is directly proportional to serum concentration. This appears to be due to a thymidine pool in rapid equilibrium with blood thymidine. This pool is at least 10 times larger than the 4-nmole pool of extracellular thymidine.  (+info)

The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. (2/13926)

We have established or characterized six lines of human breast cancer maintained in long-term tissue culture for at least 1 year and have examined these lines for estrogen responsiveness. One of these cell lines, MCF-7, shows marked stimulation of macromolecular synthesis and cell division with physiological concentrations of estradiol. Antiestrogens are strongly inhibitory, and at concentrations greater than 3 X 10(-7) M they kill cells. Antiestrogen effects are prevented by simultaneous treatment with estradiol or reversed by addition of estradiol to cells incubated in antiestrogen. Responsive cell lines contain high-affinity specific estradiol receptors. Antiestrogens compete with estradiol for these receptors but have a lower apparent affinity for the receptor than estrogens. Stimulation of cells by estrogens is biphasic, with inhibition and cell death at concentrations of 17beta-estradiol or diethylstilbestrol exceeding 10(-7) M. Killing by high concentrations of estrogen is probably a nonspecific effect in that we observe this response with 17alpha-estradiol at equivalent concentrations and in the otherwise unresponsive cells that contain no estrogen receptor sites.  (+info)

The effects of glucocorticoids and progesterone on hormone-responsive human breast cancer in long-term tissue culture. (3/13926)

Glucocorticoids, at physiological concentration, inhibit cell division and thymidine incorporation in three lines of human breast cancer maintained in long-term tissue culture. At steroid concentrations sufficient to inhibit thymidine incorporation 50%, little or no effect is seen on protein synthesis 48 hr after hormone addition. All three of these lines are shown to have glucocorticoid receptors demonstrable by competitive protein binding assays. Receptors are extensively characterized in one line by sucrose density gradient analysis and binding specificity studies. Good correlation between receptor-binding specificity and biological activity is found except for progesterone, which binds to glucocorticoid receptor but is noninhibitory. Cross-competition and quantification studies demonstrate a separate receptor for progesterone. This receptor has limited binding specificities restricted largely to progestational agents, whereas the glucocorticoid receptor bound both glucocorticoids and progesterone. Two other human breast cancer lines neither contain glucocorticoid receptor nor are inhibited by glucocorticoids. It is concluded that in some cases glucocorticoids can directly limit growth in human breast cancer in vitro without requiring alterations in other trophic hormones.  (+info)

The effects of androgens and antiandrogens on hormone-responsive human breast cancer in long-term tissue culture. (4/13926)

We have examined five human breast cancer cell lines in continuous tissue culture for androgen responsiveness. One of these cell lines shows a 2- to 4-fold stimulation of thymidine incorporation into DNA, apparent as early as 10 hr following androgen addition to cells incubated in serum-free medium. This stimulation is accompanied by an acceleration in cell replication. Antiandrogens [cyproterone acetate (6-chloro-17alpha-acetate-1,2alpha-methylene-4,6-pregnadiene-3,20-dione) and R2956 (17beta-hydroxy-2,2,17alpha-trimethoxyestra-4,9,11-triene-1-one)] inhibit both protein and DNA synthesis below control levels and block androgen-mediated stimulation. Prolonged incubation (greater than 72 hr) in antiandrogen is lethal. The MCF- cell line contains high-affinity receptors for androgenic steroids demonstrable by sucrose density gradients and competitive protein binding analysis. By cross-competition studies, androgen receptors are distinguishable from estrogen receptors also found in this cell line. Concentrations of steroid that saturate androgen receptor sites in vitro are about 1000 times lower than concentrations that maximally stimulate the cells. Changes in quantity and affinity of androgen binding to intact cells at 37 degrees as compared with usual binding techniques using cytosol preparation at 0 degrees do not explain this difference between dissociation of binding and effect. However, this difference can be explained by conversion of [3H]-5alpha-dihydrotestosterone to 5alpha-androstanediol and more polar metabolites at 37 degrees. An examination of incubation media, cytoplasmic extracts and crude nuclear pellets reveals probable conversion of [3H]testosterone to [3H]-5alpha-dihydrotestosterone. Our data provide compelling evidence that some human breast cancer, at least in vitro, may be androgen dependent.  (+info)

Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins. (5/13926)

The C-terminal G domain of the mouse laminin alpha2 chain consists of five lamin-type G domain (LG) modules (alpha2LG1 to alpha2LG5) and was obtained as several recombinant fragments, corresponding to either individual modules or the tandem arrays alpha2LG1-3 and alpha2LG4-5. These fragments were compared with similar modules from the laminin alpha1 chain and from the C-terminal region of perlecan (PGV) in several binding studies. Major heparin-binding sites were located on the two tandem fragments and the individual alpha2LG1, alpha2LG3 and alpha2LG5 modules. The binding epitope on alpha2LG5 could be localized to a cluster of lysines by site-directed mutagenesis. In the alpha1 chain, however, strong heparin binding was found on alpha1LG4 and not on alpha1LG5. Binding to sulfatides correlated to heparin binding in most but not all cases. Fragments alpha2LG1-3 and alpha2LG4-5 also bound to fibulin-1, fibulin-2 and nidogen-2 with Kd = 13-150 nM. Both tandem fragments, but not the individual modules, bound strongly to alpha-dystroglycan and this interaction was abolished by EDTA but not by high concentrations of heparin and NaCl. The binding of perlecan fragment PGV to alpha-dystroglycan was even stronger and was also not sensitive to heparin. This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly.  (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (6/13926)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

The 3' end CCA of mature tRNA is an antideterminant for eukaryotic 3'-tRNase. (7/13926)

Cytoplasmic tRNAs undergo posttranscriptional 5' and 3' end processing in the eukaryotic nucleus, and CCA (which forms the mature 3' end of all tRNAs) must be added by tRNA nucleotidyl transferase before tRNA can be aminoacylated and utilized in translation. Eukaryotic 3'-tRNase can endonucleolytically remove a 3' end trailer by cleaving on the 3' side of the discriminator base (the unpaired nucleotide 3' of the last base pair of the acceptor stem). This reaction proceeds despite a wide range in length and sequence of the 3' end trailer, except that mature tRNA containing the 3' terminal CCA is not a substrate for mouse 3'-tRNase (Nashimoto, 1997, Nucleic Acids Res 25:1148-1154). Herein, we extend this result with Drosophila and pig 3'-tRNase, using Drosophila melanogaster tRNAHis as substrate. Mature tRNA is thus prevented from recycling through 3' end processing. We also tested a series of tRNAs ending at the discriminator base (-), with one C added (+C), two Cs added (+CC), and CCA added (+CCA) as 3'-tRNase inhibitors. Inhibition was competitive with both Drosophila and pig 3'-tRNase. The product of the 3'-tRNase reaction (-) is a good 3'-tRNase inhibitor, with a KI approximately two times KM for the normal 3'-tRNase substrate. KI increases with each nucleotide added beyond the discriminator base, until when tRNA+CCA is used as inhibitor, KI is approximately forty times the substrate KM. The 3'-tRNase can thus remain free to process precursors with 3' end trailers because it is barely inhibited by tRNA+CCA, ensuring that tRNA can progress to aminoacylation. The active site of 3'-tRNase may have evolved to make an especially poor fit with tRNA+CCA.  (+info)

Daidzein and genistein glucuronides in vitro are weakly estrogenic and activate human natural killer cells at nutritionally relevant concentrations. (8/13926)

Daidzein and genistein glucuronides (DG and GG), major isoflavone metabolites, may be partly responsible for biological effects of isoflavones, such as estrogen receptor binding and natural killer cell (NK) activation or inhibition. DG and GG were synthesized using 3-methylcholanthrene-induced rat liver microsomes. The Km and Vmax for daidzein and genistein were 9.0 and 7.7 micromol/L, and 0.7 and 1.6 micromol/(mg protein. min), respectively. The absence of ultraviolet absorbance maxima shifts in the presence of sodium acetate confirmed that the synthesized products were 7-O-glucuronides. DG and GG were further purified by a Sephadex LH-20 column. DG and GG competed with the binding of 17beta-(3H) estradiol to estrogen receptors of B6D2F1 mouse uterine cytosol. The concentrations required for 50% displacement of 17beta-(3H) estradiol (CB50) were: 17beta-estradiol, 1.34 nmol/L; diethylstilbestrol, 1.46 nmol/L; daidzein, 1.6 micromol/L; DG, 14.7 micromol/L; genistein, 0.154 micromol/L; GG, 7.27 micromol/L. In human peripheral blood NK cells, genistein at <0.5 micromol/L and DG and GG at 0.1-10 micromol/L enhanced NK cell-mediated K562 cancer cell killing significantly (P < 0.05). At > 0.5 micromol/L, genistein inhibited NK cytotoxicity significantly (P < 0.05). The glucuronides only inhibited NK cytotoxicity at 50 micromol/L. Isoflavones, and especially the isoflavone glucuronides, enhanced activation of NK cells by interleukin-2 (IL-2), additively. At physiological concentrations, DG and GG were weakly estrogenic, and they activated human NK cells in nutritionally relevant concentrations in vitro, probably at a site different from IL-2 action.  (+info)

*Mixed inhibition

... seen in cases where the inhibitor favours binding to the free enzyme. More closely mimics competitive binding. An increase in ... in which the inhibitor can only bind the enzyme if the substrate has already bound. If the ability of the inhibitor to bind the ... it is known as a non-competitive inhibitor. Non-competitive inhibition is sometimes thought of as a special case of mixed ... a site different from the active site where the substrate binds. However, not all inhibitors that bind at allosteric sites are ...

*Cocaine intoxication

"Competitive binding between cocaine and lidocaine". Journal of the American College of Cardiology. 27 (2): 80. doi:10.1016/ ... Cocaine pharmacodynamics involve multiple complex mechanisms, although its half-life is short (~ 1 hour). This drug binds and ...

*Chromium(III) picolinate

Quarles, C.; Marcus, R.; Brumaghim, Julia (2011). "Competitive binding of Fe3+, Cr3+, and Ni2+ to transferrin". Journal of ... The bicarbonate ligand is crucial in binding Cr3+ as when bicarbonate concentrations are very low, the binding affinity is also ... Low-molecular-weight chromium-binding substance (LMWCr; also known as chromodulin) is an oligopeptide that seems to bind ... to bind as well. The binding sites consist of a C-lobe and an N-lobe which are nearly identical in structure. Each lobe ...

*ELISA

A third use of ELISA is through competitive binding. The steps for this ELISA are somewhat different from the first two ... The steps are: A surface is prepared to which a known quantity of capture antibody is bound. Any nonspecific binding sites on ... In between the washes, only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by ... thus ELISA falls under the bigger category of ligand binding assays. The ligand-specific binding reagent is "immobilized", i.e ...

*Theories of general anaesthetic action

Franks NP, Lieb WR (August 1984). "Do general anaesthetics act by competitive binding to specific receptors?". Nature. 310 (16 ... Thus the volume of the n-alkanol chain at the cutoff length provides an estimate of the binding site volume. This objection ... It was initially hypothesized that general anaesthetic binds to its target ion channel by a key-lock mechanism and changes its ... General anaesthetics could equally well be binding to hydrophobic target sites on proteins in the brain. The main reason that ...

*GRIN2B

Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Irie M, Hata Y, Takeuchi M, Ichtchenko K, Toyoda A, Hirao K, Takai Y, Rosahl TW, Südhof TC (September 1997). "Binding of ... a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90". Neuron. 20 (4): 693-707. doi:10.1016/s0896- ... The NR2 subunit acts as the agonist binding site for glutamate, one of the predominant excitatory neurotransmitter receptors in ...

*Actinin alpha 2

Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E (May 2000). "Binding of ADAM12, a marker of skeletal muscle ... Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E (May 2000). "Binding of ADAM12, a marker of skeletal muscle ...

*RecF pathway

Demonstration of competitive binding and the lack of a specific protein-protein interaction". Journal of Biological Chemistry. ... When present, SSBP binds to the 3' overhang remaining after RecJ finishes cutting back the 5' strand. By binding to the single- ... In the RecFOR pathway, the RecFR complex binds where the single-strand DNA of the 3' meets the double-strand DNA. RecO then ... The RecF pathway begins when RecJ, an exonuclease that cleaves single-stranded DNA in the 5 → 3′ direction, binds to the 5' end ...

*EFR (EF-Tu receptor)

This has been proven analytically through competitive binding assays and SDS-PAGE analysis. When EFR binds to EF-Tu, the basal ... Instead of binding to EF-Tu, it binds to flagellin, another highly conserved structure present on many pathogens. It, like EF- ... This receptor is an important part of the plant immune system as it allows the plant cells to recognize and bind to EF-Tu, ... They are part of the innate immune system and bind to and prevent the proliferation of pathogens with the PAMPs that they can ...

*K-server problem

... reducing the competitive ratio to k or providing an improved lower bound remains open as of 2014[update]. The most common ... a randomized algorithm with competitive bound Õ(log2k log3n) was found. To make the problem more concrete, imagine sending ... The competitive ratio of our algorithm on this input is 20,500/6000 or approximately 3.4, and by adjusting the parameters of ... 1990) first proved that there exists an algorithm with finite competitive ratio for any constant k and any metric space, and ...

*Drug test

Urine drug testing is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine ... A drug, if present in the urine specimen below its cut-off concentration, will not saturate the binding sites of its specific ... specimen compete against their respective drug conjugate for binding sites on their specific antibody. During testing, a urine ...

*Thymidylate synthase

FdUMP is able to inhibit TS by binding to the nucleotide-binding site of dUMP. This competitive binding inhibits the normal ... which acts as an antimetabolite that irreversibly inhibits TS by competitive binding. However, due to a low level of 5-FU found ... A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate). The sequence ...

*Optical mapping

Later technologies use DNA melting, DNA competitive binding or enzymatic labelling in order to create the optical mappings. The ... "Competitive binding-based optical DNA mapping for fast identification of bacteria - multi-ligand transfer matrix theory and ...

*2-Methoxyestradiol

Thekkumkara T, Snyder R, Karamyan VT (2016). "Competitive Binding Assay for the G-Protein-Coupled Receptor 30 (GPR30) or G- ... It also acts as a vasodilator.[citation needed] 2-ME2 is derived from estradiol, although it binds poorly (2000-fold lower ...

*2-Hydroxyestradiol

Thekkumkara, Thomas; Snyder, Russell; Karamyan, Vardan T. (2016). "Competitive Binding Assay for the G-Protein-Coupled Receptor ... Moreover, 2-hydroxyestradiol has been found to bind to the α1-adrenergic receptor with slightly more than half the affinity of ... It was noted that this could be due to 2-hydroxyestradiol binding to and antagonizing the D2 receptor. However, the researchers ... Kuiper GG, Carlsson B, Grandien K, Enmark E, Häggblad J, Nilsson S, Gustafsson JA (1997). "Comparison of the ligand binding ...

*Butylparaben

... displayed the most competitive binding to rat estrogen receptors when tested along with methyl, ethyl, and ...

*Nuclear receptor

However they block the effect of agonist through competitive binding to the same binding site in the nuclear receptor. These ... Type IV nuclear receptors bind either as monomers or dimers, but only a single DNA binding domain of the receptor binds to a ... C) DNA-binding domain (DBD): Highly conserved domain containing two zinc fingers that binds to specific sequences of DNA called ... Binding of antagonist ligands to nuclear receptors in contrast induces a conformation of the receptor that preferentially binds ...

*Arachidonate 5-lipoxygenase

"Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ... may serve as a mobile lid over ALOX5's substrate-binding site An Adenosine triphosphate (ATP) binding site; ATP is crucial for ... The binding of ALOX5 to membranes as well as its interaction with FLAP likewise cause the enzyme to alter its relative levels ...

*GRB2

"Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... The C-terminal SH3 domain binds to peptides conforming to a P-X-I/L/V/-D/N-R-X-X-K-P motif that allows it to specifically bind ... Zamora-Leon SP, Lee G, Davies P, Shafit-Zagardo B (Oct 2001). "Binding of Fyn to MAP-2c through an SH3 binding domain. ... The N-terminal SH3 domain binds to proline-rich peptides and can bind to the Ras-guanine exchange factor SOS. ...

*Irazepine

It is a non-competitive benzodiazepine binding site antagonist. Irazepine and other alkylating benzodiazepine, kenazepine, bind ... to brain benzodiazepine receptors in a non-competitive (covalent) fashion in vitro, and may exert a long-lasting anticonvulsant ...

*Adduct

... upon Small-Molecule Binding and the Competitive Binding of CO and Ethylene". Journal of the American Chemical Society. 128 (41 ...

*Antibiotic use in livestock

MOS works as a competitive binding site, as bacteria bind to it rather than the intestine and are carried out. Bacteriophages ... In another study it was found that using probiotics, competitive exclusion, enzymes, immunomodulators and organic acids ...

*Quorum sensing

... through its competitive binding to LsrR. Glyceraldehyde 3-phosphate has also been shown to inhibit the lsr operon through cAMP- ... When gram-positive bacteria detect high concentration of AIP in the environment, AIP bind to a receptor to activate kinase. The ... Another possible mechanism is that AIP is transported into the cytosol, and binds directly to a transcription factor to ... Usually AHLs do not need additional processing, and bind directly to transcription factors to regulate gene expression. Some ...

*Dissociation constant

Acid Equilibrium constant Ki Database Competitive inhibition pH Scatchard plot Ligand binding Bisswanger, Hans (2008). Enzyme ... Then, the concentration of bound ligands [ L ] bound {\displaystyle {{\ce {[L]_{bound}}}}} becomes [ L ] bound = n [ M ] 0 [ L ... bound = [ LM ] + 2 [ L 2 M ] + 3 [ L 3 M ] + ⋅ ⋅ ⋅ + n [ L n M ] {\displaystyle {\ce {[L]_{bound}={[LM]}+{2[L_{2}M]}+{3[L_{3}M ... though the derivation can be extended to explicitly allow for and describe competitive binding.[citation needed] It is useful ...

*WDFW Toxics in Biota fish monitoring

Quantification of vitellogenin is performed by measuring competitive binding to an enzyme in the presence of another ...

*Preselection

eds), Democracy at the Polls: A Comparative Study of Competitive national Elections, American Enterprise Institute, Washington ... Eligibility to be a candidate in preselection is frequently bound by rules set by a political party. Preselection may also be ...
如左圖所示,兩根曲線所代表的分別是兩種與受體結合親和性不同的配體。配體對受體的結合能力通常是由半數的受體結合位點被配體佔據時的濃度表徵的,該濃度稱爲IC50(英语:IC50),與解離常數Kd有關但有區別。圖中紅色曲線所表示的配體比起綠色曲線所表示的配體有更高的結合親和性和更小的Kd值。如果這兩種配體同時存在的話,那麼高親和性的配體與受體結合更多。這也就是一氧化碳能優先於氧氣與血紅蛋白結合,從而導致一氧化碳中毒的原理。. 配體對受體的結合親和性通常藉由用放射性同位素標記的配體来確定,這種配體稱爲「熱配體」。「同源競爭性結合實驗」(Homologous competitive binding experiments)的原理就是讓帶有放射性的「熱配體」和不帶放射性的「冷配體」競爭結合位點[4]。不過,表面等離子體共振和雙偏振干涉(英语:dual polarisation ...
BioAssay record AID 438342 submitted by ChEMBL: Binding affinity to FLT3 catalytic domain expressed in HEK293 cells by competitive binding assay.
Competitive binding assay. Clone R24.1 and clone R23.1 mabs did not block the binding of anti-CD30 (BerH2 antibody) to KMH2 cells.
Endothelial cells separate the bloodstream from the underlying tissue and play a crucial role in vascular homeostasis. They also form an important barrier for vascular drug delivery. This thesis contains preliminary studies targeted at understanding the mechanisms of binding and transport across endothelial cells cultured in vitro. Specifically, the first study investigates how the recombinant source of Factor IX (FIX), a blood coagulant protein used in the treatment of Hemophilia B, impacts surface ligand binding (FIX to its specific receptors) to bovine aortic endothelial cells (BAECs). Competitive binding experiments between 125I-FIX and FIX were undertaken to quantify the interaction of recombinant and transgenic FIX with BAECs and human collagen IV and determine if there was a measurable difference in binding affinity. Results indicate limited specific binding of 125I-FIX to BAECs and no binding to human collagen IV. Concrete conclusions were not drawn from this data due to technical issues ...
TY - JOUR. T1 - Development of a theoretical model for chromatographic-based competitive binding immunoassays with simultaneous. T2 - Injection of sample and label. AU - Hage, D. S.. AU - Thomas, D. H.. AU - Chowdhuri, A. R.. AU - Clarke, W.. PY - 1999/8/17. Y1 - 1999/8/17. N2 - This study examined the theory and behavior of an HPLC-based chromatographic competitive binding immunoassay with the simultaneous injection of sample and a labeled analyte analogue. Equations based on nonlinear chromatographic theory were derived to describe the calibration curve for this assay in a system with adsorption-limited kinetics and homogeneous binding sites. These equations related the assay response (B/B(o)) to the columns binding capacity, the moles of injected analyte or labeled analogue, and the flow rate/adsorption kinetics of the system. There was good agreement between the predicted theoretical response and experimental data obtained for the binding of human serum albumin (HSA) to an immobilized ...
low capacity battery manufacturer/supplier, China low capacity battery manufacturer & factory list, find qualified Chinese low capacity battery manufacturers, suppliers, factories, exporters & wholesalers quickly on Made-in-China.com.
BioAssay record AID 146424 submitted by ChEMBL: The receptor binding activity was evaluated as the displacement of specifically bound [3H]-NPY from male Sprague-Dawley rat brain membrane homogenates.
VPC 32183(S) is a competitive antagonist at the LPA1 and LPA3 receptors. VPC 32183 is devoid of agonist activity at the human LPA1, LPA2 and LPA3 receptors, and
A system for very sensitive detection and/or quantification of an analyte is disclosed. The system is based on an interaction, such as specific binding or competitive displacement from a ligand, betwe
In this article, we have identified a single Arg-to-Leu substitution in BNP that increases the EC50 for GC-A 7-fold and reduces the EC50 for GC-B by 5-fold. In contrast, a Met-for-Ser mutation in the second divergent triad had no effect on activation of either cyclase. A peptide containing both substitutions (LM-BNP) behaved like l-bnp in the whole-cell activation assay, although in broken-cell assays, l-bnp was a better activator of both cyclases, consistent with the notion that l-bnp is more stable under these conditions than LM-BNP. Competition binding experiments indicated that the increased EC50 for GC-A was caused by reduced binding of l-bnp to the receptor. The analogous CNP binding experiments were not conducted because of the lack of availability of a high-quality 125I-CNP tracer, but it is likely that the reduced EC50 for GC-B results from affinity changes as well. It is noteworthy that the affinity of wild-type BNP and l-bnp to NPR-C was not significantly different, which indicates ...
where \(V_{0}\) is the volume of the cell and \(V_{i}\) is the volume of the \(i\)-th injection.. pytc calculates \(x_{PA,i}\) and friends for the entire titration, correcting for dilution. This means the \(f_{i}\) term is superfluous. Thus, heats are related by:. ...
Low Capacity Portable Generator Market Insights 2019, is a professional and in-depth study on the current state of the global. ...
The KINOMEscan assay platform is based on a competition binding assay that is run for a compound of interest against each of a panel of 317 to 456 kinases. The assay has three components: a kinase-tagged phage, a test compound, and an immobilized ligand that the compound competes with to displace the kinase. The amount of kinase bound to the immobilized ligand is determined using quantitative PCR of the DNA tag. Results for each kinase are reported as "Percent of control", where the control is DMSO and where a 100% result means no inhibition of kinase binding to the ligand in the presence of the compound, and where low percent results mean strong inhibition. The KINOMEscan data are presented graphically on TREEspot Kinase Dendrograms (http://www.kinomescan.com/Tools---Resources/Study-Reports---Data-Analysis). For this study, HMS LINCS investigators have graphed results for kinases classified as 35 "percent of control" (in the presence of the compound, the kinase is 35% as active for binding ...
binding to the DR-1/AP-1 site in suppressing MMP-1 and MMP-13 production and addressed possible mechanisms of inhibition, including competitive binding between RXR:PPAR ...
Relative affinities of XRCC1 proteins for PARP-1 (A), LIG3α (B) and PCNA (C). Shown are the average and standard deviation of three independent affinity captur
The binding of a number of fluorescent probe molecules to human serum albumin (HSA) has been studied. Small changes in the amino acid moiety of the dansylamino acids resulted in large changes in the binding of these compounds to HSA. It is suggested that electrostatic and dipolar forces play a role in the specificity and binding affinity of such compounds. Fluorescent probes which had one tight binding site were used for drug displacement studies. Changes in probe fluorescence were shown, by equilibrium dialysis and by fluorescence titrations, to be a result of competitive displacement by drugs. The pattern of displacement of probes by drugs enabled the identification of two specific drug binding sites. The relative affinity of drugs for these binding sites was measured by their ability to displace fluorescent probes specific for the sites. The method provides a rapid and simple means for detecting potential drug interactions based on competition for protein binding sites.. ...
We have compared the cancer cell cytotoxicity, cell uptake., and DNA binding properties of the isomeric terphenyl complexes [(eta(6)-arene)Ru(en)Cl](+), where the arene is ortho- (2), meta- (3), or para-terphenyl (1) (o-, m-, or p-terp). Complex 1, the X-ray crystal structure of which confirms that it has the classical "piano-stool" geometry, has a similar potency to cisplatin but is not cross-resistant and has a much higher activity than 2 or 3. The extent of Ru uptake into A2780 or A2780cis cells does not correlate with potency. Complex I binds to DNA rapidly and quantitatively, preferentially to guanine residues, and causes significant DNA unwinding. Circular and linear dichroism, competitive binding experiments with ethidium bromide, DNA melting, and surface-enhanced Raman spectroscopic data are consistent with combined intercalative and monofunctional (coordination) binding mode of complex 1. This unusual DNA binding mode may therefore make a major contribution to the high potency of ...
A patients health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patients health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable
Auto Flood Reservoir Conditions Coreflooding System with Miscible Gas Module is a semi-automated, modular Core Flooding system that is configured for unsteady-state relative permeability tests. Core Flooding experiments in single and multi phase displacements are available in manual and semi-automatic mode, addition of the gas delivery system allow performance of gas displacement experiments. This system is specifically configured to take advantage of Core Laboratories 50 years of performing waterflooding and simulation experiments. The base system is configured for liquid/liquid displacements under unsteady state conditions and the addition of the gas delivery system allows Gas/Liquid displacement experiments under unsteady state conditions or miscible flood experiments to be performed. The system is rated to 10,000 psig confining pressure, 6,000 psig pore pressure at 300 °F temperature. The system features automated data acquisition, manual and semi-automated operation via a Windows based ...
The novel AMPA antagonist RPR 119990 has a potent affinity for the rat AMPA receptor in membrane-binding studies that compares favorably with results for other AMPA receptor antagonists described in the literature (Takahashi et al., 1998; Turski et al., 1998). The compound was selective with respect to other ionotropic glutamate receptors, although a certain affinity for the kainate-binding site at around 50-fold higher concentrations was noted. The compound shows low or negligible affinity for 37 other binding or uptake sites, suggesting strong specificity of action. The activity on the closely related kainite site is expected, because cross-reactivity has already been reported (Bleakman and Lodge, 1998) and may account for some of the compounds anticonvulsant and neuroprotective actions.. RPR 119990 acts as a competitive antagonist at the recombinant human AMPA receptor/channel expressed in X. laevis oocytes. The compound shows a profile compatible with a competitive single site antagonism of ...
Low Capacity Battery Price - Select 2017 high quality Low Capacity Battery Price products in best price from certified Chinese Ups Sla Battery manufacturers, 18v Battery suppliers, wholesalers and factory on Made-in-China.com
We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPgS data is also provided for some of our Gi coupled receptors ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Clearly, the reliability of the relative affinity of a candidate ligand relies on the validity of Eq.(6), and requires the simultaneous satisfaction of the following prerequisites for the pair of the candidate ligand and its reference ligand. (a) The candidate ligand and its reference ligand bind to the same site(s) on the target. In practice, the binding site(s) of the candidate ligand can be judged based on its competitive binding against a reference ligand but can not be optimized. (b) The candidate ligand and its reference ligand, in both the PMFS and the concentrated extract, produce peak areas within their own linear ranges. (c) The candidate ligand and its reference ligand, in both the PMFS and the concentrated extract, produce peak areas over five times the absolute values of their own intercepts of linear response. (d) The candidate ligand and its reference ligand have binding ratios of below 10% in the competitive binding system. All the later three prerequisites should be met by ...
Intelligencer staffers discuss how Democratic candidates should handle a major vulnerability for front-runner Joe Biden: His age.
Competitive binding assay definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up now!
Infections with the four serotypes of mosquito-borne dengue virus (DENV-1-4) are one the rise;it is currently estimated that almost 400 million people are infec...
Introduction: Competitive binding assays can be used to decipher not only the binding kinetics of studied ligands but also the binding site preference. Such assays are an essential step in the characterization of radioligands. However, the currently used competition assays require high concentrations of usually expensive ligands and still provide only binding site preference. By employing the time-resolved competition assay presented in this paper, binding characteristics including binding site preference can be obtained using less ligand. Methods: To demonstrate the appropriateness of the time-resolved competition assay, we developed an assay in which the ligand binding was interrupted with a competitor. Experiments were performed on human carcinoma cell lines expressing epidermal growth factor receptor (EGFR). The targeting of the receptor was performed with radio-iodinated epidermal growth factor (EGF). The employed competitors involved either natural ligand transforming growth factor alpha ...
Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPgS data is also provided for some of our Gi coupled receptors.. Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximity methods, such as FlashPlate, or classical filtration methods). Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Certificate of Analysis.. Some of our receptors may be restricted for sale in specified ...
Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors.. We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTP?S data is also provided for some of our Gi-coupled receptors.. Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtration methods).. Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet.. Some of our receptors may be restricted for sale in ...
testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi-coupled receptors.. Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtration methods).. Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet.. Some of our receptors may be restricted for sale in specified countries. Please inquire.. ...
testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi-coupled receptors.. Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtration methods).. Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet.. Some of our receptors may be restricted for sale in specified countries. Please inquire.. ...
Leukotriene (LT) C4, LTD4 and LTE4 have positive inotropic effects on contractility of the isolated perfused bullfrog heart. The effects of LTD4 and LTE4 but not LTC4 can be blocked by the mammalian antagonist L-649,923. Characterization of specific binding sites for [3H]LTC4 on membrane preparations from American bullfrog (Rana catesbeiana) ventricle was carried out. Binding assays were done in the presence of serine (5 mM) and borate (10 mM) for 30 min at 23 degrees C. Under these conditions, no metabolism of LTC4 to LTD4 occurred. Specific binding of [3H]LTC4 reached steady state within 10 min, remained constant for 60 min, and was reversible with the addition of 1000-fold excess unlabeled LTC4. Scatchard analysis of the binding data indicated a single class of binding sites with a Kd of 33.9 nM and maximal binding capacity of 51.6 pmol/mg of protein. Competition binding studies revealed an order of potency of LTC4 greater than LTD4 greater than LTE4 with Ki values of 47, 11766 and 32248 nM, ...
3H-Spiroperidol labels multiple high affinity states with serotonergic selectivity in human prefrontal cortex and with dopaminergic selectivity in human caudate and putamen. The characteristics of...
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. ...
The title should never, and I mean never, be the driver to choose a course or a degree. Once you start to see how problems are set up and how to approach them, youll see that you can apply those same principles to a lot of the Chem E classes you take and things get easier. Experiential learning through competitions and/or field work is a major component of the class. The approach is generally more macroscopic and applied than in condensed matter physics. If you think this is an error, please contact the webmaster ...
The in vitro and in vivo binding characteristics of [I-125] iodomethyllycaconitine ([I-125]iodoMLA) were determined in the rat. [I-125]iodoMLA binding to rat cerebral cortex membranes was saturable and reversible and its specific binding represented approximately 70-80% of the total binding. [I-125]iodoMLA labeled a single site with K-d = 1.8 +/- 0.4 nM and B-max = 68 +/- 3 fmol/mg protein. Kinetic analysis revealed a t(1/2) for association and dissociation of 10.5 +/- 3.1 and 10.3 +/- 1.6 min, respectively.
Keeping abreast of the competition is a well-known business mantra. However, how can you do that without understanding what your competitors are up to?
We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPgS data is also provided for some of our Gi coupled receptors ...
In competitive inhibition, an inhibitor that resembles the normal substrate binds to the enzyme, usually at the active site, and prevents the substrate from binding.[8] At any given moment, the enzyme may be bound to the inhibitor, the substrate, or neither, but it cannot bind both at the same time. During competitive inhibition, the inhibitor and substrate compete for the active site. The active site is a region on an enzyme which a particular protein or substrate can bind to. The active site will only allow one of the two complexes to bind to the site therefore either allowing for a reaction to occur or yielding it. In competitive inhibition the inhibitor resembles the substrate therefore taking its place and binding to the active site of an enzyme. Increasing the substrate concentration would diminish the "competition" for the substrate to properly bind to the active site and allow a reaction to occur.[3] When the substrate is of higher concentration than that of the competitive inhibitor, it ...
Deactivation of bacteria for competition study - posted in Microbiology: Hi all, Im a new user in this forum and register specifically to ask this question; Im doing fungal fermentation. Every now and then I do have some contamination but it wasnt serious. I didnt use any antibiotics as my fungus is usually strong enough to overcome the contamination (I do practice sterile techniques) Ive been only analysing the one metabolite produce by the fungus (metabolite A)...
Blakeley, D, Sykes, DA, Ensor, P, Bertran, E, Aston, PJ and Charlton, SJ (2015) Simulating the influence of plasma protein on measured receptor affinity in biochemical assays reveals the utility of Schild analysis for estimating compound affinity for plasma proteins ...
Learn about Phyto Chem (India) Competition, get detailed comparison of Phyto Chem (India) with major competitors in terms of market cap, sales, net profit and assets.
Learn about Unichem Laboratories Competition, get detailed comparison of Unichem Laboratories with major competitors in terms of market cap, sales, net profit and assets.
PHILADELPHIA---- Sixty communities-- 10 each from six geographic regions-- have been named finalists in a national competition to find the
THE COAGULATION CASCADE CONSISTS OF A NUMBER OF MULTIDOMAIN SERINE PROTEASES AND PROTEIN COFACTORS. AMONG THESE ARE FACTORS IX, X, AND PROTHROMBIN. PROTEIN C DOWNREGULATES THE INTRINSIC COAGULATION PATHWAY BY INACTIVATING FACTORS VAAND VIIIA. FACTORS IX, X, AND PROTEIN C ARE HIGHLY HOMOLOGOUS PROTEINS. EACH CONTAINS TWO EGF-HOMOLOGOUS DOMAINS AND A N-TERMINAL DOMAIN BEARING MULTIPLE -CARBOXYGLUTAMIC ACID RESIDUES, THE SO-CALLED GLA DOMAIN. COMPETITIVE BINDING EXPERIMENTS HAVE ESTABLISHED THAT FACTORS IX AND X BIND TO RECEPTORS ON ACTIVATED ENDOTHELIAL CELLS THROUGH INTERACTIONS OF THE EGF AND GLA DOMAINS, A PHYSIOLOGICAL MECHANISM FOR DIRECTING COAGULATION TO REGIONS OF DAMAGED ENDOTHELIUM. THE OBJECTIVE IS TO IMPROVE RECOMBINANT PROTEIN C FOR USE ASA SAFE ANTICOAGULANT/ANTITHROMBOTIC AGENT FOR SUPPRESSION OF CLOTTING IN A NUMBER OF VENOUS THROMBOTIC CONDITIONS. SPECIFICALLY, A PROTEIN C VARIANT WITH A HIGH BINDING AFFINITY FOR DAMAGED ENDOTHELIUM WILL BE DESIGNED. THE EGF-HOMOLOGOUS DOMAINS OF ...
cAMP signals are locally amplified by scaffold proteins (A Kinase Anchor Proteins, AKAPs) that tether cAMP-dependent Protein Kinase A (PKA) to discrete cellular locations. Here we hypothesized that mitochondrial anchoring of PKA promotes survival in muscle cells. We identified AKAP121 as the major mitochondrial AKAP in cardiomyocytes and aortic smooth muscle cells. In response to pressure overload, cardiac AKAP121 levels were significantly reduced, inducing marked mitochondrial dysfunction, DNA damage and activation of the DNA repair machinery. To test the role of AKAP121 in the modulation of cell survival, we synthesized peptides (AK-in) containing AKAP121 mitochondrial targeting domain but lacking its PKA binding motif, in order to competitively displace the endogenous AKAP121/PKA complex from mitochondria. Sequence-scrambled peptides were synthetized and used as controls (S). 24 hours after administration, FITC-conjugated AK-in peptides co-localized with mitochondria at confocal microscopy; ...
Ligand binding assays (LBA) is an assay, or an analytic procedure, whose procedure or method relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. A detection method is used to determine the presence and extent of the ligand-receptor complexes formed, and this is usually determined electrochemically or through a fluorescence detection method. This type of analytic test can be used to test for the presence of target molecules in a sample that are known to bind to the receptor. There are numerous types of ligand binding assays, both radioactive and non-radioactive. As such, ligand binding assays are a superset of radiobinding assays, which are the conceptual inverse of radioimmunoassays (RIA). Some newer types are called "mix-and-measure" assays because they do not require separation of bound ligands. Ligand binding assays are used primarily in pharmacology for various demands. Specifically, despite the human bodys endogenous receptors, hormones, and other ...
The design of molecular probes and tracer molecules with specificity toward amyloid beta (A beta) fibrils is of paramount importance for the selective diagnosis of Alzheimers disease. This requires a detailed understanding of the binding sites in amyloid targets, their number, and their binding mechanism for various tracer molecules. We adopt an integrated approach including molecular docking, molecular dynamics, and generalized Born-based free energy calculations to investigate site-specific interactions of different amyloid binding molecules. Our study reproduces the experimental results on the relative binding affinity of the tracers and amyloid binders and explains the feature of "multiple binding sites" in amyloid targets as probed by competition binding experiments. A major outcome of this study is that it is the core sites of the Afi fibrils that are responsible for the experimentally reported binding profiles of tracers in amyloid targets rather than the surface sites that received much ...
This work describes the fluorescence enhancement of the anilinonaphthalene sulfonate probes 1,8-ANS, 2,6-ANS, and 2,6-TNS via complexation with PAMAM dendrimer hosts of Generation 4, 5 and 6. The use of this set of three very closely related probes allows for comparative binding studies, with specific pairs of probes differing only in shape (1,8-ANS and 2,6-ANS), or in the presence of a methyl substituent (2,6-TNS vs. 2,6-ANS). The fluorescence of all three probes was significantly enhanced upon binding with PAMAM dendrimers, however in all cases except one, a very unusual spike was consistently observed in the host fluorescence titration plots (fluorescence enhancement vs. host concentration) at low dendrimer concentration. This unprecedented fluorescence titration curve shape makes fitting the data to a simple model such as 1:1 or 2:1 host: guest complexation very difficult; thus only qualitative comparisons of the relative binding of the three guests could be made based on host titrations. In the
Affinity Chromatography (AC) is based on the specific adsorption of a molecule to a ligand or macromolecule. Most biomolecules can be purified on the basis of specific interaction between their chemical or biological structure and a suitable affinity ligand. Typical molecular pairs are enzymes and coenzymes or antigens and antibodies. AC packing materials have spacer ligands that are first attached to the substrate before a reversible adsorption of a specific biomolecule. The adsorbed molecule is then eluted through a competitive displacement or by a change in the conformation of the molecule through a change in pH or ionic strength. In contrast to other chromatographic methods, AC is highly selective and is mostly suitable for specific separation problems. Separation Methods Technologies silica-based Chemically Immobilized Biomolecules (CIB) columns are for high performance purification of other biomolecules such as proteins, enzymes and antibodies. In production of CIB columns, SMT utilizes its
The Chemical and Chemical Engineering Resource Guide is the database dedicated to chemical engineers, helping them find the products & services they need.Biolin Scientific,Burg Translations, Inc.,De Gruyter,Labsciences, Inc.,MicroSurfaces, Inc.,Molecular Dimensions,Norgen Biotek Corp.,
IMMUNE SUPPORT: take 1 Pure Defense™ daily.. DURING COLD & FLU SEASON: take 1-2 Pure Defense™ daily to support your immune response.. BEFORE TRAVEL: take 1-3 Pure Defense™ daily for at least 1 week before a trip and more intensively during international travel.. WORK: when co-workers are under the weather, take 1-2 Pure Defense™ daily to maintain optimal immune health.. STRESS: incorporate a daily Pure Defense™ into the supplementation regimen when under stress, preparing for competition or stress-inducing activities.. AT THE FIRST SIGN OF IMMUNE STRESS: take 2-4 Pure Defense™ daily.. ATHLETES: to maintain optimal health all the way through competition, take 1 Pure Defense™ daily during training & competition.. ...
TF (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human TF in plasma and serum. (KA0509) - Products - Abnova
Looking for pocket tissue competitor? Here you can find the lowest price products about pocket tissue competitor. We Provide for you about pocket tissue competitor page1
RS 39604 hydrochloride is a potent and selective 5-HT4 antagonist, with a pKi of 9.1 at 5-HT4 receptors in guinea pig striatal membranes and greater than 1000-fold selectivity over 5-HT1A, 2C, 3 and D1, D2, M1, M2, AT1, B1 and α1C receptors. Learn More ...
Compare your products vs. competitors at the chemical level. Breakdown product formulations identifying the actionable strengths and weaknesses in each product
Studies of the mechanisms by which DNA polymerases select the correct nucleotide frequently employ fluorescently labeled DNA to monitor conformational rearrangements of the polymerase-DNA complex in response to incoming nucleotides. For this purpose, fluorescent base analogs play an increasingly important role because they interfere less with the DNA-protein interaction than do tethered fluorophores. Here we report the incorporation of the 5-triphosphates of two exceptionally bright cytosine analogs, 1,3-diaza-2-oxo-phenothiazine (tC) and its oxo-homolog, 1,3-diaza-2-oxo-phenoxazine (tC(O)), into DNA by the Klenow fragment. Both nucleotide analogs are polymerized with slightly higher efficiency opposite guanine than cytosine triphosphate and are shown to bind with nanomolar affinity to the DNA polymerase active site, according to fluorescence anisotropy measurements. Using this method, we perform competitive binding experiments and show that they can be used to determine the dissociation constant of
Total internal reflection fluorescence (TIRF) and ellipsometry have been used to study competitive protein adsorption to a hydrophobic model surface prepared by radio frequency plasma deposition of hexamethyl disiloxane on silicon. Single, binary, and ternary protein solutions of human serum albumin (HSA), IgG, and fibrogen (Fgn) at concentrations corresponding to 1/100 of those in blood plasma were investigated. It is shown that by employing the combination of ellipsometry and TIRF, information on both the total adsorbed amount and the composition of the adsorbed protein layer can be obtained. It was found that adsorbed HSA is not displaced by IgG and/or Fgn to any large extent. IgG and HSA dominate the adsorption from the ternary protein mixture, although fibrinogen is also present in the adsorbed layer to a smaller extent.. ...
CLICK HERE. Naltrexone long acting formulations and methods of use. Naltrexone is a medication primarily used in the management of alcohol dependence and Naltrexone is also used in formulation with bupropion The Sinclair method buy transderm scop online is a method of using opiate antagonists such as naltrexone to treat . Naltrexone and its active metabolite 6β-naltrexol are competitive antagonists at theNov 16, 2011 The inventions also include a method of treating an individual in buy betnovate ointment ottawa need of naltrexone comprising administering a long acting formulation in aApr 6, 2005 Abstract; Methods; Results; Comment; Article Information; References .. safety of 2 dosing levels of this long-acting injectable formulation of naltrexone in The naltrexone buy provigil online in mississauga long-acting injection used in this study consisted ofLong-acting naltrexone depot formulations also are designed to minimize the high After the administration of a competitive mu-receptor ...
TY - JOUR. T1 - A computational model of the LGI1 protein suggests a common binding site for ADAM proteins. AU - Leonardi, Emanuela. AU - Andreazza, Simonetta. AU - Vanin, Stefano. AU - Busolin, Giorgia. AU - Nobile, Carlo. AU - Tosatto, Silvio C E. PY - 2011/3/29. Y1 - 2011/3/29. N2 - Mutations of human leucine-rich glioma inactivated (LGI1) gene encoding the epitempin protein cause autosomal dominant temporal lateral epilepsy (ADTLE), a rare familial partial epileptic syndrome. The LGI1 gene seems to have a role on the transmission of neuronal messages but the exact molecular mechanism remains unclear. In contrast to other genes involved in epileptic disorders, epitempin shows no homology with known ion channel genes but contains two domains, composed of repeated structural units, known to mediate protein-protein interactions. A three dimensional in silico model of the two epitempin domains was built to predict the structure-function relationship and propose a functional model integrating ...
The interaction between Np(V) and human aposerumtransferrin (apoTf) was studied in vitro under simulated physiological conditions (37°C, pH 7.4, [NaCl] = 0.15 M, [HEPES] = 5 × 10-2 M) by UV-visible and near-IR absorption spectrophotometry and by ultrafiltration. It was found that Np(V) was bound in small fraction (, 12%) to apoTf, and that the role of carbonate and citrate anions in binding is more competitive than synergistic. The complexes NpO2CO3- and NpO2Cit2- tend to form rather than the Np(V)-apoTf complex. Np(V) binding on apotransferrin appears to be pH-dependent and reversible. Displacement experiments with FeIIINTA showed non-specific binding, suggesting a weak interaction between Np(V) and apotransferrin different from the reactions involving transferrin-specific sites with other metallic ions, such as Fe(III). The low overall charge, the size and the geometry of the linear di-oxocation NpO2+ could account for the weak interaction between Np(V) and human transferrin ...
Autor: Richter, Klaus et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2003; Titel: Sti1 is a non-competitive inhibitor of the Hsp90 ATPase - Binding prevents the N-terminal dimerization reaction during the ATPase cycle
We synthesized the new supramolecular host molecules 7 and 13 based on functionalized resorcarenes bearing Kemps triacid. Conformations in solution have been investigated and the influence of intra-and intermolecular hydrogen bonds from the triacid was shown by low temperature and DOSY NMR experiments. The results of host 7 are supported by DFT calculations. The binding behaviour of 7 towards different 2-amino pyridincs in chloroform has been investigated by NMR titrations. The association constants reach from K 207 M-1 for 2-amino-5-cyano pyridine to K=1551 M-1 for 2-amino-4-methyl pyridine. The association constants of the formed complexes of 7 with 2-amino pyridines were compared with those of the simple host systems 14 and 15 in order to evaluate the influence of the attached resorcarene host. (c) 2008 Elsevier Ltd. All rights reserved ...
Binding of the cage convulsant, [3H]TBOB, to sites linked to the GABAA receptor complex. Article date: 1990/4/25 PubMed ID: 2163855 Journal name: European journal of pharmacology (ISSN: 0014-2999) ABSTRACT [3H]t-Butylbicycloorthobenzoate ([3H]TBOB) binds to specific sites on crude synaptic rat brain membranes. The dissociation constant, Kd, determined from saturation experiments is near 8 nM and the receptor density Bmax is about 20 pmol/g wet tissue. Non-specific binding constitute…
Sigma receptors, both Sigma‐1(S1R) and Sigma‐2 (S2R), are small molecule‐regulated, primarily endoplasmic reticulum (ER) membrane‐associated sites
A cantilever device based on competitive binding of an immobilized receptor to immobilized and soluble ligand and capable of measuring solution-phase thermodynamic quantities is described. Through multiple binary queries, the device stochastically measures the probability of the formation of a bound complex between immobilized protein and immobilized ligand as a function of soluble ligand concentration. The resulting binding isotherm is described by a binding polynomial consisting of the activities of soluble and immobilized ligand and binding constants for the association of immobilized protein with free and immobilized ligand. Evaluation of the polynomial reveals an association constant for the formation of a complex between immobilized ligand and immobilized protein close to that for the formation of complex between soluble protein and soluble ligand. The methodology lays the foundation for construction of practical portable sensing devices ...
... ,The procedure follows the basic principle of a competitive enzyme immunoassay where there is competition between an unlabeled antigen and an labelled antigen bound to the limited binding sites of a specific antiserum . The amount of enzyme conjugate complex (HRP-avidin: biotin-labelled estrone) bou,medicine,medical supply,medical supplies,medical product
Introduction. QAW039 is an oral, selective, competitive and reversible CRTh2 receptor antagonist currently in clinical development for treatment of asthma. This study describes the detailed investigation of the receptor binding kinetics of QAW039 and compares it to several other known CRTh2 antagonists.. Methods. Kinetic binding experiments with [3H3]-QAW039 and CRTh2 receptor were carried out at 37oC, [35S]-GTPγS binding at room temperature1. The ability of compounds to inhibit the whole blood shape change (WBSC) response to PGD2 was monitored using flow cytometry.. Results. The dissociation rate constant for QAW039 was significantly lower than all other clinically relevant CRTh2 antagonists tested (QAV680, OC459 and AZD-1981, Table). QAW039 was the most potent compound tested in the WBSC assay. In the binding assay QAW039 significantly reduced the maximum effect of PGD2 following a 15min incubation. ...
Cytokines are soluble extracellular proteins or glycoproteins that are crucial intercellular regulators and mobilizers of cells engaged in innate as well as adaptive inflammatory host defenses, cell growth, differentiation, cell death, angiogenesis, and development and repair processes aimed at the restoration of homeostasis. Cytokines are released by various cells in the body, usually in response to an activating stimulus, and they induce responses through binding to specific receptors on the cell surface of target cells. Cytokines can be grouped by structure into different families and their receptors can likewise be grouped ...
Cytokines are soluble extracellular proteins or glycoproteins that are crucial intercellular regulators and mobilizers of cells engaged in innate as well as adaptive inflammatory host defenses, cell growth, differentiation, cell death, angiogenesis, and development and repair processes aimed at the restoration of homeostasis. Cytokines are released by various cells in the body, usually in response to an activating stimulus, and they induce responses through binding to specific receptors on the cell surface of target cells. Cytokines can be grouped by structure into different families and their receptors can likewise be grouped ...
The Chloramphenicol EIA test is a competitive enzyme immunoassay for the screening of chloramphenicol in tissue, plasma, serum, urine, eggs, milk, honey and
Global antibody supplier and research reagent supplier to the life science community. Find antibodies and reagents all backed by our Guarantee+.
Gentaur molecular products has all kinds of products like :search , Genesee \ 200ul Pipet Tip, Reload Clear, Low Binding \ 24-150RL for more molecular products just contact us
If the kinetic data for an inhibitor do not match any of the above patterns, the inhibitor may act in a mode referred to as mixed inhibition. In this scenario, the inhibitor can bind to both E and ES, but with different affinities. In this case, there are two Kis (one for the dissociation of EI and one for the dissociation of ESI) that are related to each other by a variable, α. In cases of mixed inhibition, the Km is usually increased and the Vmax is usually decreased in comparison to the values for the uninhibited reaction. A typical Lineweaver-Burk plot for mixed inhibition is shown on the right below. It is not possible to calculate a Ki value for this type of inhibition with the data gathered in this lab. If you think your data suggest a mixed inhibition mechanism, you should determine with which of the other modes of inhibition they are most consistent and use that formula to calculate a Ki value. Notice that, when the variable α is very large, the mechanism of inhibition approaches ...
... pharmaceutical market-Training Center,Bid information-Zhejiang Yatai Pharmaceutical Co., Ltd.: Competition from pharmaceutical companies to enter the marke
List of words make out of Noncompetitive. Anagrams of word Noncompetitive. Words made after scrabbling Noncompetitive. Word Creation helps in Anagrams and Puzzles.
Complete Genomics competitors and comparable companies (comps) include Helicos BioSciences, OpGen and 14 others. You can see Complete Genomicss complete list of competitors with funding history here.
Die folgenden Programme wurden in der 64 und 32 Byte Intro Competition der 0a000h 2003 veröffentlicht. Aus Platzgründen enthält die 32b-Version keine Tastaturabfrage ...
Distribution of responsibility, ability and competition . . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
If you want to contact me, or you have a product that is suitible for my family and you would like me to review it, then you can Contact Me at [email protected] ...
This study characterises the somatostatin binding site in human gastrointestinal cancer and mucosa in terms of cationic specificity and relative affinity for three somatostatin analogues. Competitive displacement assays were performed on plasma membranes from human gastric and colonic tissues using radiolabelled somatostatin-14 as ligand. Comparison was made with the somatostatin binding site in rat cerebral cortex. In gastrointestinal tissue, magnesium decreased and sodium increased specific binding. By contrast, in rat cerebral cortex, the converse cationic effect was seen. These changes resulted from alterations in receptor density, with no change in receptor affinity. Displacement studies were then performed with somatostatin-14 and somatostatin analogues RC-160, somatuline, and octreotide. RC-160 and somatuline displaced radiolabel from binding sites in gastric and colonic cancer and mucosa with 10-fold lower affinity than the native peptide. Octreotide did not displace radioligand in ...
Sarver R.W., Gao H., Tian F.. An NMR method was developed for determining binding sites of small molecules on human serum albumin (HSA) by competitive displacement of (13)C-labeled oleic acid. This method is based on the observation that in the crystal structure of HSA complexed with oleic acid, two principal drug-binding sites, Sudlows sites I (warfarin) and II (ibuprofen), are also occupied by fatty acids. In two-dimensional [(1)H,(13)C]heteronuclear single quantum coherence NMR spectra, seven distinct resonances were observed for the (13)C-methyl-labeled oleic acid as a result of its binding to HSA. Resonances corresponding to the major drug-binding sites were identified through competitive displacement of molecules that bind specifically to each site. Thus, binding of molecules to these sites can be followed by their displacement of oleic acids. Furthermore, the amount of bound ligand at each site can be determined from changes in resonance intensities. For molecules containing fluorine, ...
TY - JOUR. T1 - Modulation of forskolin binding to rat brain membranes. AU - Seamon, K. B.. AU - Vaillancourt, Richard. AU - Daly, J. W.. PY - 1985. Y1 - 1985. N2 - High affinity binding sites for [3H]forskolin have been identified in rat brain membranes. These sites have a K(d) of 15 nM and a B(max) of about 200 fmol/mg protein. The binding of [3H]forskolin to those high affinity sites in rat brain membranes is increased about two-fold by addition of MgCl2 or MnCl2. Smaller increases are observed in the presence of calcium, sodium, or potassium. The binding of [3H]forskolin is also increased in the presence of NaF or GppNHp, agents that are known to activate adenylate cyclase through the stimulatory guanine nucleotide regulatory protein (N(s)). The increase in [3H]forskolin binding in the presence of NaF or GppNHp is due to an increase in the number of binding sites with no change in the apparent K(d) for the binding sites. The NaF- and GppNHp-stimulated binding requires the presence of ...
Aguilera, R.. Relative Permeability Concepts for Predicting the Performance of Naturally Fractured. Reservoirs. JCan PetT,.22,41, (1982). Amaefule, J.O. and Handy, L.I., The Effect oflnterfacial Tensions on Relative Oil/Water Permeabilities of Consolidated Porous Media. Soc Pet E J, 371, (1982). Bardon, C. and Longeron, D., Influence of Very Low Interfacial Tensions on Relative Permeability. Soc Pet EJ, 20, 391,(1980). Batycky, J.P., Mirkin, M.I., Besserer, G.J., Jackson, C.H., Miscible and Immiscible Displacement Studies on Carbonate Reservoir Cores. JCanPetT,2L, 104,(1981A). Batycky, J.P., McCaffery, F.G., Hodgins, P.K., Fisher, D.B., Interpreting Relative Permeability and. Wettability from Unsteady-State Displacement Measurements. Soc Pet E J, 21, 296, (198IB). Bemsten, R.G., Using Parameter Estimation Techniques to Convert Centrifuge Data into a Capillary. Pressure Curve. SPE paper 5026 presented at the SPE-AIME 49th Annual Meeting, Houston, Texas, (Oct. 6-9 1974). Broadbent, S.R. and ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Zinc atom in PDB 1zef: Structure of Alkaline Phosphatase From Human Placenta in Complex With Its Uncompetitive Inhibitor L-Phe
Believing that transatlantic economic integration will maximize economic benefits for their citizens through competition and stronger growth, while maintaining high standards of safety and protection, at their last summit meeting in April 2007, the leaders of the European Union (EU) and the United States of America (United States) committed their governments to increasing the efficiency and transparency of transatlantic economic cooperation and to accelerating the reduction and elimination of barriers to international trade and investment with the ultimate objective of achieving a barrier free transatlantic market.
Using this strategy, we calculated log IC50 values from the resulting inhibition curves. Greater than a half log increase in IC50 was observed for both the IE and gB targets of ddPCR over qPCR for both SDS (absolute log difference in IC50 qPCR vs ddPCR IE, 0.554, and vs ddPCR gB, 0.628) and heparin (absolute log difference in IC50 qPCR vs ddPCR IE, 0.655, and vs ddPCR gB, 0.855). The probability of difference between the data sets for ddPCR and qPCR was ,99.99% for both inhibitors and both ddPCR targets, indicating that ddPCR tolerated the presence of these inhibitors better than qPCR. However, this difference was not noted when we compared ddPCR and qPCR in the presence of EDTA for both ddPCR targets (log difference in IC50 qPCR vs ddPCR IE, 0.116, and vs ddPCR gB, 0.0198), possibly owing to different inhibition mechanisms. EDTA is a calcium chelator, whereas SDS and heparin both act on DNA polymerase.. The ddPCR CMV assay is more tolerant to SDS and heparin than the qPCR assay, indicating that ...
Unfortunately, no organization works in isolation and the FMGC industry in particular is extremely competitive. With the wipe market alone set to grow by 6.5% per year on average, it is important that you have strategies in place to ensure that you remain competitive over the long term. Whether you are the market leader or a new entrant, its vital that you do everything possible to remain at the forefront of your customers minds by offering them the perfect wipe products. So how do you make sure that you hold your own and climb the market share ranks against your competitors?. 1. Know your competitors and what they are offering. Its vital to understand as much about your closest competitors as possible. The information gleaned from regular and in-depth competitor research can be vital to gaining the upper hand and improving your own products. What are your competitors doing better than you? What are their future plans for their wipe and related product offerings? Who are they targeting, how ...
Numerous studies during the past decade have demonstrated that certain pharmacological antagonists act through a relatively stable combination with specific receptors. This action cannot be considered to be truly irreversible. However, it is qualitatively different from classical competitive antagonism in that the agonist and antagonist are not in mass-action equilibrium with the receptors. The blockade produced may therefore be referred to as nonequilibrium. It is clearly different from ninecompetitive antagonism in which the antagonist acts at some point other than the site of action of the agonist in question. Nonequilibrium blockade cannot be adequately distinguished from competitive or noncompetitive blockade on the basis of effects on the agonist dose-response curves, or on the basis of duration of action. However, satisfactory differentiation can be made by combination of tests, several of which depend upon the fact that the persistence of a competitive blockade appears to ve limited by ...
An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. Th
1O42: Requirements for specific binding of low affinity inhibitor fragments to the SH2 domain of (pp60)Src are identical to those for high affinity binding of full length inhibitors.
Impossible Foods competitors and comparable companies (comps) include Beyond Meat, Modern Meadow and 1 others. You can see Impossible Foodss complete list of competitors with funding history here.
If you have a canine that you would like to begin showing at showmanship competitions, you will have to start training your dog quite early. Many competitors have been doing this for years, and will be accustomed to the atmosphere.
As AgriLIVE Smithfield draws nearer this week is your last chance to enter the competition to win a Musto jacket courtesy of the supreme champion sponsors
So nadbl is coming to Danbury NH or Sunday, and I will be attending this event for the first time...I read up on it and it doesnt say anything about the ...
The relative binding affinity of 5 alpha-reduced progestins and a newly synthesized antiprogestin J912 (progesterone 100%) was determined in a competitive receptor binding assay using [3H]ORG-2058 as radiolabeled ligand for the progestin receptor. Uteri obtained from 12 different species of four mammalian orders were examined. The relative binding affinity of 75-100% and a blood prevalence of 5 alpha-pregnane-3,20-dione in horses and African elephants suggest a biological role of this particular 5 alpha-reduced progesterone. For pigs the binding affinity of 5 alpha-pregnane-3,20-dione was about 50% of progesterone, but blood levels are unknown. In all other cases the low binding affinity of investigated progestins precludes possible biological role. For 5 alpha-pregnane-3 alpha-ol-20-one, 5 alpha-pregnane-20 alpha-ol-3-one, and 5 alpha-pregnane-3 beta,20 alpha-diol the relative binding affinity was less than 1%. A rather low binding (| 15%) was observed in 5 alpha-pregnane-3,20-dione in all ruminant
Cinnarizine is an antihistamine and a calcium channel blocker. Histamines mediate a number of activities such as contraction of smooth muscle of the airways and gastrointestinal tract, vasodilatation, cardiac stimulation, secretion of gastric acid, promotion of interleukin release and chemotaxis of eosinophils and mast cells. Competitive antagonists at histamine H1 receptors may be divided into first (sedating) and second (non-sedating) generation agents. Some, such as Cinnarizine also block muscarinic acetylcholine receptors and are used as anti-emetic agents. Cinnarizine through its calcium channel blocking ability also inhibits stimulation of the vestibular system ...
Cinnarizine is an antihistamine and a calcium channel blocker. Histamines mediate a number of activities such as contraction of smooth muscle of the airways and gastrointestinal tract, vasodilatation, cardiac stimulation, secretion of gastric acid, promotion of interleukin release and chemotaxis of eosinophils and mast cells. Competitive antagonists at histamine H1 receptors may be divided into first (sedating) and second (non-sedating) generation agents. Some, such as Cinnarizine also block muscarinic acetylcholine receptors and are used as anti-emetic agents. Cinnarizine through its calcium channel blocking ability also inhibits stimulation of the vestibular system ...
From: NAME: Les Davies FUNC: Therapeutic Goods Admin TEL: (06)289 7182 - MDP 88 ,DAVIES LES at [email protected], To: mx%bioforum at [email protected] at [email protected] Message-id: D752IOD2V16Y From: NAME: Les Davies FUNC: Therapeutic Goods Admin TEL: (06)289 7182 - MDP 88 ,DAVIES LES at [email protected], Subject: (1) Estrogen receptor assays & (2) the nervous system Date: 04-Nov-1996 Posted-date: 04-Nov-1996 Precedence: 1 To: mx5biosci-request at [email protected] at [email protected] (1) Estrogen receptor assays Noted a reply to Raymond Pierre (from JS Amenta?) about receptor binding assays for estrogen receptors - the comment that the point is to inhibit non-specific binding and not affect the specific receptor binding is not really correct - non-specific binding is not inhibitable. In receptor binding assays, an excess of a known receptor binding compound is added (to a separate set of assay tubes from the control binding tubes) to make sure that specific receptors are fully saturated and then no tritiated label ...
TY - JOUR. T1 - Impact of neonatal treatment with cardioactive glycosides (digoxin, ouabain) on receptor binding capacity, blood level and cardiac function in the adult rat. Extension of the imprinting theory. AU - Csaba, G.. AU - Inczefi-Gonda, Ágnes. AU - Dobozy, O.. AU - Varró, A.. AU - Rablóczky, G.. PY - 1983. Y1 - 1983. N2 - 1. 1. A single neonatal treatment with a cardioactive glycoside (ouabain, digoxin) altered the response of the adult rat to digitaloid treatment. 2. 2. As demonstrated by RIA, re-exposure to digoxin at 2 months of age was followed within 30 min by a more than twofold increase in serum digoxin over the not pretreated control and, although a steady concentration decrease followed, the experimental rats still had a higher serum digoxin level than the controls at 4 h. 3. 3. In the not presensitized control group the serum digoxin peak appeared at 60 min at a considerably lower level than the 30-min maximum of the experimental rats. 4. 4. Neonatal pretreatment with ...
To study the cost of chromosomal drug resistance mutations to bacteria, we investigated the fitness cost of mutations that confer resistance to different classes of antibiotics affecting bacterial protein synthesis (aminocyclitols, 2-deoxystreptamines, macrolides). We used a model system based on an in vitro competition assay with defined Mycobacterium smegmatis laboratory mutants; selected mutations were introduced by genetic techniques to address the possibility that compensatory mutations ameliorate the resistance cost. We found that the chromosomal drug resistance mutations studied often had only a small fitness cost; compensatory mutations were not involved in low-cost or no-cost resistance mutations. When drug resistance mutations found in clinical isolates were considered, selection of those mutations that have little or no fitness cost in the in vitro competition assay seems to occur. These results argue against expectations that link decreased levels of antibiotic consumption with the ...

Competitive binding assay | Define Competitive binding assay at Dictionary.comCompetitive binding assay | Define Competitive binding assay at Dictionary.com

Competitive binding assay definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. ... competitive binding assay in Medicine Expand. competitive binding assay com·pet·i·tive bind·ing assay (kəm-pětĭ-tĭv bīndĭng) ... An assay in which a biologically specific binding agent competes for radioactively labeled or unlabeled compounds, used ...
more infohttp://www.dictionary.com/browse/competitive-binding-assay

Competitive Binding TechnologyCompetitive Binding Technology

... Active-site/Ligand Binding Technology Overview. DiscoverXs proprietary active-site/ligand ... InCELL Hunter™ Intracellular Binding Assay Platform. Employing EFC, the catalytic domain of the protein of interest is tagged ... BROMOscan℠ Bromodomain-ligand Binding Platform. Based on the proven KINOMEscan℠ technology platform, BROMOscan℠ employs a novel ... KINOMEscan℠ Active-site Directed Competition Binding Platform. The KINOMEscan screening platform employs a novel and ...
more infohttps://www.discoverx.com/technologies-platforms/competitive-binding-technology

Competitive binding assay - Biology-Online DictionaryCompetitive binding assay - Biology-Online Dictionary

Competitive binding assay general term for an assay in which a binder competes for labelled versus unlabelled ligand; following ... Competitive binding assay. Revision as of 21:16, 3 October 2005 by WikiConvertor (Talk) ... separation of free and bound ligand, the ligand (the analyte assayed) is quantitated by relating bound and unbound ratios to ... Retrieved from "http://www.biology-online.org/bodict/index.php?title=Competitive_binding_assay&oldid=28463" ...
more infohttp://www.biology-online.org/bodict/index.php?title=Competitive_binding_assay&oldid=28463

EPR studies of intermolecular interactions and competitive binding of drugs in a drug-BSA binding model.  - PubMed - NCBIEPR studies of intermolecular interactions and competitive binding of drugs in a drug-BSA binding model. - PubMed - NCBI

EPR studies of intermolecular interactions and competitive binding of drugs in a drug-BSA binding model.. Akdogan Y1, ... indicating the importance of electrostatic interaction in drug binding. Moreover, the competitive binding properties of ... On the other hand, aspirin replaces only ∼23% of bound SL-salicylic acid, and salicylic acid replaces ∼50% of bound SL-aspirin ... of bound SL-salicylic acid, and salicylic acid can replace only ∼14% of the bound SL-ibuprofen. This indicates that ∼97% of all ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/27468942

Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria.Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria.

Binding, Competitive. The interaction of two or more substrates or ligands with the same binding site. The displacement of one ... Summary of "Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria.". Soil is a ... Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria.. 08:00 EDT 4th September ... Home » Topics » Environmental Health » Research » Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites ...
more infohttps://www.bioportfolio.com/resources/pmarticle/2155879/Competitive-binding-of-Cd-Ni-and-Cu-on-goethite-organo-mineral-composites.html

LanthaScreen TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit - Thermo Fisher ScientificLanthaScreen TR-FRET PXR (SXR) Competitive Binding Assay Kit, rabbit - Thermo Fisher Scientific

Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the ... competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. ... Competitive ligand binding is detected by a test compounds ability to displace a fluorescent ligand (tracer) from the receptor ... The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput ...
more infohttps://www.thermofisher.com/order/catalog/product/A15142

Electrostatic Interactions of Peptides with Lipid Membranes: Competitive Binding between Cationic Peptides and Divalent...Electrostatic Interactions of Peptides with Lipid Membranes: Competitive Binding between Cationic Peptides and Divalent...

This effort enables us to study the competitive binding between cationic peptides and divalent counterions. Our results offer a ... Electrostatic Interactions of Peptides with Lipid Membranes: Competitive Binding between Cationic Peptides and Divalent ... Electrostatic Interactions of Peptides with Lipid Membranes: Competitive Binding between Cationic Peptides and Divalent ... physical explanation for the observed preferred binding of cationic antimicrobial peptides onto the outer leaflet of Gram- ...
more infohttps://uwspace.uwaterloo.ca/handle/10012/5574?show=full

Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and...Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and...

... evidence of epistasis and competitive binding.. [Katherine E Burdick, Atsushi Kamiya, Colin A Hodgkinson, Todd Lencz, Pamela ... In addition, we report opposing binding patterns of NDEL1 and NDE1 to Ser704 versus Cys704, at the same DISC1 binding domain. ... Finally, in a series of in vitro assays, we determined the binding profiles of NDEL1 and NDE1, in relation to DISC1 Ser704Cys. ... Intact functions of DISC1 and its binding partners, NDEL1 and NDE1, are critical to neurodevelopmental processes aberrant in ...
more infohttps://www.sigmaaldrich.com/catalog/papers/18469341

Chemokine Cooperativity Is Caused by Competitive Glycosaminoglycan Binding | The Journal of ImmunologyChemokine Cooperativity Is Caused by Competitive Glycosaminoglycan Binding | The Journal of Immunology

Chemokine cooperativity is caused by competitive GAG binding. The simplest model that explains the necessity for GAG binding in ... Radioligand binding. Radioligand-binding experiments were performed, as described previously (3). For saturation-binding ... Chemokine Cooperativity Is Caused by Competitive Glycosaminoglycan Binding. Folkert Verkaar, Jody van Offenbeek, Miranda M. C. ... Chemokine Cooperativity Is Caused by Competitive Glycosaminoglycan Binding. Folkert Verkaar, Jody van Offenbeek, Miranda M. C. ...
more infohttp://www.jimmunol.org/content/192/8/3908

Nucleosome Free Regions in Yeast Promoters Result from Competitive Binding of Transcription Factors That Interact with...Nucleosome Free Regions in Yeast Promoters Result from Competitive Binding of Transcription Factors That Interact with...

Thus, the picture that emerges is that binding by a specific class of TFs recruits chromatin modifiers which mediate local ... On the other hand, larger nucleosome free regions (NFRs) in promoters are explained predominantly by TF binding. Strikingly, we ... As nucleosome positioning profoundly affects DNA accessibility to other DNA binding proteins such as transcription factors (TFs ... biophysical model to systematically study the relative contributions of intrinsic sequence preferences and competitive binding ...
more infohttps://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003181

Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria, Environmental Pollution |...Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria, Environmental Pollution |...

"Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria, Environmental Pollution" on ... Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria. Competitive binding of Cd, ... Competitive binding of Cd, Ni and Cu on goethite organo-mineral composites made with soil bacteria. Du, Huihui; Huang, Qiaoyun ... lp/elsevier/competitive-binding-of-cd-ni-and-cu-on-goethite-organo-mineral-8vdW0CCFkh ...
more infohttps://www.deepdyve.com/lp/elsevier/competitive-binding-of-cd-ni-and-cu-on-goethite-organo-mineral-8vdW0CCFkh?key=bioportfolio

Bargaining, binding contracts and competitive wagesBargaining, binding contracts and competitive wages

... Westermark, Andreas Uppsala University, Humanistisk-samhällsvetenskapliga ... Bargaining; Decreasing returns; Competitive wages National Category Economics Identifiers. URN: urn:nbn:se:uu:diva-46732OAI: ... In a model where many workers bargain with one firm and sign binding contracts, we show existence of a stationary subgame ...
more infohttp://uu.diva-portal.org/smash/record.jsf?pid=diva2:74639

Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers |...Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers |...

Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. ... Probing the binding site of a new synthesized anti-cancer compound to HSA via competitive ligand binding method. J Mol Liq 206: ... Probing the Interaction of Newly Synthesized Pt(II) Complex on Human Serum Albumin Using Competitive Binding Site Markers. ... The number of binding sites and binding constants were calculated at both temperatures of 25 and 37 °C. In addition, in order ...
more infohttps://rd.springer.com/article/10.1007%2Fs10895-019-02383-3

Endogenous Angiotensins, Angiotensin Ii-Competitive Binding Inhibitors and Converting Enzyme Inhibitor in Elasmobranch Fish  » ...Endogenous Angiotensins, Angiotensin Ii-Competitive Binding Inhibitors and Converting Enzyme Inhibitor in Elasmobranch Fish » ...

The AII competitive binding inhibitors [Sar1-Val5-Ala8]-AII and [Sar1-IIe8]-AII did not inhibit the pressor action of dogfish ... The AII competitive binding inhibitors [Sar1-Val5-Ala8]-AII and [Sar1-IIe8]-AII did not inhibit the pressor action of dogfish ... Endogenous Angiotensins, Angiotensin Ii-Competitive Binding Inhibitors and Converting Enzyme Inhibitor in Elasmobranch Fish ... Endogenous Angiotensins, Angiotensin Ii-Competitive Binding Inhibitors and Converting Enzyme Inhibitor in Elasmobranch Fish ...
more infohttp://booksandjournals.brillonline.com/content/journals/10.1163/156854295x00717

Detection of Growth Hormone Releasing Peptides in Serum by a
Competitive Receptor Binding Assay | OMICS InternationalDetection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay | OMICS International

Purified samples were reconstituted in 150 μl of binding buffer and analysed by the competitive binding assay (see above). ... Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay Ferro P1*, Krotov G2, Zvereva I ... 2017)Detection of Growth Hormone Releasing Peptides in Serum by a Competitive Receptor Binding Assay. J Chromatogr Sep Tech 8: ... Two reference samples in binding buffer were included in each competition binding experiment, one as blank or negative control ...
more infohttps://www.omicsonline.org/open-access/detection-of-growth-hormone-releasing-peptides-in-serum-by-acompetitive-receptor-binding-assay-2157-7064-1000351.php?aid=86342

The kinetics of competitive radioligand binding predicted by the law of mass action. | Molecular PharmacologyThe kinetics of competitive radioligand binding predicted by the law of mass action. | Molecular Pharmacology

The kinetics of competitive radioligand binding predicted by the law of mass action.. H J Motulsky and L C Mahan ... The kinetics of competitive radioligand binding predicted by the law of mass action.. H J Motulsky and L C Mahan ... The kinetics of competitive radioligand binding predicted by the law of mass action.. H J Motulsky and L C Mahan ... The kinetics of competitive radioligand binding predicted by the law of mass action. ...
more infohttp://molpharm.aspetjournals.org/content/25/1/1/tab-article-info

Competitive calcium ion binding to end-tethered weak polyelectrolytes - Soft Matter (RSC Publishing)Competitive calcium ion binding to end-tethered weak polyelectrolytes - Soft Matter (RSC Publishing)

Competitive calcium ion binding to end-tethered weak polyelectrolytes R. Nap, S. H. Park and I. Szleifer, Soft Matter, 2018, ...
more infohttp://pubs.rsc.org/en/content/articlelanding/2018/sm/c7sm02434g

According to the competitive-binding ELISA results, t - livertoll icuAccording to the competitive-binding ELISA results, t - livertoll icu

HomeUncategorizedAccording to the competitive-binding ELISA results, t. According to the competitive-binding ELISA results, t. ...
more infohttp://livertoll.icu/according-to-the-competitive-binding-elisa-results-t/

Competitive binding assay. Clone R24.1 and clone R23.1  | Open-iCompetitive binding assay. Clone R24.1 and clone R23.1 | Open-i

Clone R24.1 and clone R23.1 mabs did not block the binding of anti-CD30 (BerH2 antibody) to KMH2 cells. ... Figure 5: Competitive binding assay. Clone R24.1 and clone R23.1 mabs did not block the binding of anti-CD30 (BerH2 antibody) ... Figure 5: Competitive binding assay. Clone R24.1 and clone R23.1 mabs did not block the binding of anti-CD30 (BerH2 antibody) ... to bind to KMH2 cells (Figure 5). Similarly, none of the anti-CD30 antibodies blocked the binding of clone R23.1 mab or clone ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2267462_1476-4598-7-12-5&req=4

Estimation of 25-hydroxyvitamin D in blood serum using competitive binding with proteinEstimation of 25-hydroxyvitamin D in blood serum using competitive binding with protein

... ... Estimation of 25-hydroxyvitamin D in blood serum using competitive binding with protein, Voprosy meditsinskoi khimii, 1983, vol ... Blood serum from rats, maintained on a ration free of vitamin D, was used as a binding system. Evaluation of the procedure was ...
more infohttp://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-1983-29-3-130-en

Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands ~ DNP-NMR Literature BlogAffinity screening using competitive binding with fluorine-19 hyperpolarized ligands ~ DNP-NMR Literature Blog

Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands Posted by Thorsten Maly ... Kim, Y. and C. Hilty, Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands. Angew Chem Int Ed ... Under a competitive equilibrium with a selected fluorinated reporter ligand, the dissociation constant (K(D)) of other ligands ... Monte Carlo simulations show that the highest accuracy is obtained when about one-half of the bound reporter ligand is ...
more infohttps://blog.bridge12.com/2015/10/affinity-screening-using-competitive.html
  • An assay in which a biologically specific binding agent competes for radioactively labeled or unlabeled compounds, used especially to measure the concentration of hormone receptors in a sample by introducing a radioactively labeled hormone. (dictionary.com)
  • Complementation between the ePL and EA occurs only when compounds bind to the catalytic domain and stabilizes the protein of interest, increasing its half-life relative to the unbound protein. (discoverx.com)
  • Such conditions can be achieved over a wide range of affinities, allowing for rapid screening of non-fluorinated compounds when a single fluorinated ligand for the binding pocket of interest is known. (bridge12.com)
  • A small library of triazine and phenylurea compounds has been synthesized which have tail-like substituents in order to test the effects of charge, hydrophobicity and size of the tail on binding properties. (elsevier.com)
  • Similarly, none of the anti-CD30 antibodies blocked the binding of clone R23.1 mab or clone R24.1 mab to KMH2 cells (data not shown). (nih.gov)
  • DiscoverX's proprietary active-site/ligand binding technology affords investigators the ability to leverage a growing portfolio of services against multiple target classes and maximixe the value of novel and chemistry assets through all stages of drug discovery. (discoverx.com)
  • When longer hydrophobic tails are used, the binding penalty that occurs upon adding a charged substituent at the distal end is reduced. (elsevier.com)
  • On the other hand, aspirin replaces only ∼23% of bound SL-salicylic acid, and salicylic acid replaces ∼50% of bound SL-aspirin, indicating that ∼73% of all salicylic acid and aspirin binding sites are shared. (nih.gov)
  • Chemokine receptor activation mediates leukocyte chemotaxis toward lymphoid organs or sites of inflammation along a chemokine gradient that is established by binding of chemokines to membrane-tethered and extracellular matrix-associated glycosaminoglycans (GAGs) ( 4 ). (jimmunol.org)
  • Competitive binding test results have shown that Pt(II) complex bind on the warfarin binding site (or Sudlow sites I) on HSA. (springer.com)
  • Curry S, Mandelkow H, Brick P, Franks N (1998) Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites. (springer.com)
  • Intact functions of DISC1 and its binding partners, NDEL1 and NDE1, are critical to neurodevelopmental processes aberrant in schizophrenia (SZ). (sigmaaldrich.com)
  • The salicylic acid loading capacity of cBSA is significantly higher compared to that of BSA, indicating the importance of electrostatic interaction in drug binding. (nih.gov)
  • An experimental adaptation of a competitive methodology for GHRPs wide-group detection, previously developed for urine, is reported here using a serum matrix instead. (omicsonline.org)
  • Salicylic acid was labeled with a stable radical (spin label) in order to monitor its mobilized (free) or immobilized (bound to BSA) states. (nih.gov)
  • Blood serum from rats, maintained on a ration free of vitamin D, was used as a binding system. (msk.ru)
  • In a model where many workers bargain with one firm and sign binding contracts, we show existence of a stationary subgame perfect equilibrium. (diva-portal.org)
  • Such a chain does not appear to be long enough to extend from the bulk aqueous phase to the Q B site because binding is completely lost when a large hydrophilic domain (PEG 4000 ) is attached to the distal end. (elsevier.com)
  • abstract = "The QB quinone-binding site of photosystem II is an important target for herbicides. (elsevier.com)
  • Kragh-Hansen U, Watanabe H, Nakajou K, Iwao Y, Otagiri M (2006) Chain length-dependent binding of fatty acid anions to human serum albumin studied by site-directed mutagenesis. (springer.com)
  • The Q B quinone-binding site of photosystem II is an important target for herbicides. (elsevier.com)
  • The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). (thermofisher.com)
  • Under a competitive equilibrium with a selected fluorinated reporter ligand, the dissociation constant (K(D)) of other ligands of interest is measurable using a single-scan Carr-Purcell-Meiboom-Gill (CPMG) experiment, without the need for a titration. (bridge12.com)