BH3 Interacting Domain Death Agonist Protein: A member of the Bcl-2 protein family that reversibly binds MEMBRANES. It is a pro-apoptotic protein that is activated by caspase cleavage.Proto-Oncogene Proteins c-bcl-2: Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.bcl-2-Associated X Protein: A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)X-Linked Inhibitor of Apoptosis Protein: An inhibitor of apoptosis protein that is translated by a rare cap-independent mechanism. It blocks caspase-mediated cellular destruction by inhibiting CASPASE 3; CASPASE 7; and CASPASE 9.Mitochondrial Membranes: The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).Cytochromes c: Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.bcl-2 Homologous Antagonist-Killer Protein: A multi-domain mitochondrial membrane protein and member of the bcl-2 Protein family. Bak protein interacts with TUMOR SUPPRESSOR PROTEIN P53 and promotes APOPTOSIS.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Cytochrome c Group: A group of cytochromes with covalent thioether linkages between either or both of the vinyl side chains of protoheme and the protein. (Enzyme Nomenclature, 1992, p539)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Genes, bcl-2: The B-cell leukemia/lymphoma-2 genes, responsible for blocking apoptosis in normal cells, and associated with follicular lymphoma when overexpressed. Overexpression results from the t(14;18) translocation. The human c-bcl-2 gene is located at 18q24 on the long arm of chromosome 18.bcl-X Protein: A member of the bcl-2 protein family that plays a role in the regulation of APOPTOSIS. Two major isoforms of the protein exist due to ALTERNATIVE SPLICING of the BCL2L1 mRNA and are referred to as Bcl-XS and Bcl-XL.Electroosmosis: The motion of a liquid through a membrane (or plug or capillary) consequent upon the application of an electric field across the membrane. (Oxford Dictionary of Biochemistry and Molecular Biology, 2001)Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Hippocampus: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.Infarction, Middle Cerebral Artery: NECROSIS occurring in the MIDDLE CEREBRAL ARTERY distribution system which brings blood to the entire lateral aspects of each CEREBRAL HEMISPHERE. Clinical signs include impaired cognition; APHASIA; AGRAPHIA; weak and numbness in the face and arms, contralaterally or bilaterally depending on the infarction.Neuroprotective Agents: Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.Biuret: Used as feed supplement for sheep and cattle since it is a good non-protein nitrogen source. In strongly alkaline solution biuret gives a violet color with copper sulfate.Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.Cytochromes: Hemeproteins whose characteristic mode of action involves transfer of reducing equivalents which are associated with a reversible change in oxidation state of the prosthetic group. Formally, this redox change involves a single-electron, reversible equilibrium between the Fe(II) and Fe(III) states of the central iron atom (From Enzyme Nomenclature, 1992, p539). The various cytochrome subclasses are organized by the type of HEME and by the wavelength range of their reduced alpha-absorption bands.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Cytochrome P-450 Enzyme System: A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.Lupus Nephritis: Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).Lupus Erythematosus, Systemic: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Autoantibodies: Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.Mice, Inbred DBAPlasmapheresis: Procedure whereby plasma is separated and extracted from anticoagulated whole blood and the red cells retransfused to the donor. Plasmapheresis is also employed for therapeutic use.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.Mice, Inbred C57BLNod Signaling Adaptor Proteins: Cytosolic signaling adaptor proteins that were initially discovered by their role in the innate immunity (IMMUNITY, INNATE) response of organisms that lack an adaptive immune system. This class of proteins contains three domains, a C-terminal ligand recognition domain, an N-terminal effector-binding domain, and a centrally located nuclear-binding oligomerization domain. Many members of this class contain a C-terminal leucine rich domain which binds to PEPTIDOGLYCAN on the surface of BACTERIA and plays a role in pathogen resistance.Inflammasomes: Multiprotein complexes that mediate the activation of CASPASE-1. Dysregulation of inflammasomes has also been linked to a number of autoinflammatory and autoimmune disorders.Nod1 Signaling Adaptor Protein: A NOD-signaling adaptor protein that contains a C-terminal leucine-rich domain which recognizes bacterial PEPTIDOGLYCAN. It signals via an N-terminal caspase recruitment domain that interacts with other CARD SIGNALING ADAPTOR PROTEINS such as RIP SERINE-THEONINE KINASES. It plays a role in the host defense response by signaling the activation of CASPASES and the MAP KINASE SIGNALING SYSTEM.Receptors, Pattern Recognition: A large family of cell surface receptors that bind conserved molecular structures (PAMPS) present in pathogens. They play important roles in host defense by mediating cellular responses to pathogens.Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Caspase 1: A long pro-domain caspase that has specificity for the precursor form of INTERLEUKIN-1BETA. It plays a role in INFLAMMATION by catalytically converting the inactive forms of CYTOKINES such as interleukin-1beta to their active, secreted form. Caspase 1 is referred as interleukin-1beta converting enzyme and is frequently abbreviated ICE.Autophagy: The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.Collagen Type IV: A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.Quantitative Trait Loci: Genetic loci associated with a QUANTITATIVE TRAIT.Collagen Type I: The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.Genome-Wide Association Study: An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.Thymoma: A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)Polymorphism, Single Nucleotide: A single nucleotide variation in a genetic sequence that occurs at appreciable frequency in the population.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Receptors, Death Domain: A family of cell surface receptors that signal via a conserved domain that extends into the cell CYTOPLASM. The conserved domain is referred to as a death domain due to the fact that many of these receptors are involved in signaling APOPTOSIS. Several DEATH DOMAIN RECEPTOR SIGNALING ADAPTOR PROTEINS can bind to the death domains of the activated receptors and through a complex series of interactions activate apoptotic mediators such as CASPASES.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Receptors, TNF-Related Apoptosis-Inducing Ligand: Tumor necrosis factor receptor family members that are widely expressed and play a role in regulation of peripheral immune responses and APOPTOSIS. The receptors are specific for TNF-RELATED APOPTOSIS-INDUCING LIGAND and signal via conserved death domains that associate with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Cell Line: Established cell cultures that have the potential to propagate indefinitely.

Bid-induced conformational change of Bax is responsible for mitochondrial cytochrome c release during apoptosis. (1/724)

Here we report that in staurosporine-induced apoptosis of HeLa cells, Bid, a BH3 domain containing protein, translocates from the cytosol to mitochondria. This event is associated with a change in conformation of Bax which leads to the unmasking of its NH2-terminal domain and is accompanied by the release of cytochrome c from mitochondria. A similar finding is reported for cerebellar granule cells undergoing apoptosis induced by serum and potassium deprivation. The Bax-conformational change is prevented by Bcl-2 and Bcl-xL but not by caspase inhibitors. Using isolated mitochondria and various BH3 mutants of Bid, we demonstrate that direct binding of Bid to Bax is a prerequisite for Bax structural change and cytochrome c release. Bcl-xL can inhibit the effect of Bid by interacting directly with Bax. Moreover, using mitochondria from Bax-deficient tumor cell lines, we show that Bid- induced release of cytochrome c is negligible when Bid is added alone, but dramatically increased when Bid and Bax are added together. Taken together, our results suggest that, during certain types of apoptosis, Bid translocates to mitochondria and binds to Bax, leading to a change in conformation of Bax and to cytochrome c release from mitochondria.  (+info)

Solution structure of BID, an intracellular amplifier of apoptotic signaling. (2/724)

We report the solution structure of BID, an intracellular cross-talk agent that can amplify FAS/TNF apoptotic signal through the mitochondria death pathway after Caspase 8 cleavage. BID contains eight alpha helices where two central hydrophobic helices are surrounded by six amphipathic ones. The fold resembles poreforming bacterial toxins and shows similarity to BCL-XL although sequence homology to BCL-XL is limited to the 16-residue BH3 domain. Furthermore, we modeled a complex of BCL-XL and BID by aligning the BID and BAK BH3 motifs in the known BCL-XL-BAK BH3 complex. Additionally, we show that the overall structure of BID is preserved after cleavage by Caspase 8. We propose that BID has both BH3 domain-dependent and -independent modes of action in inducing mitochondrial damage.  (+info)

Solution structure of the proapoptotic molecule BID: a structural basis for apoptotic agonists and antagonists. (3/724)

Members of the BCL2 family of proteins are key regulators of programmed cell death, acting either as apoptotic agonists or antagonists. Here we describe the solution structure of BID, presenting the structure of a proapoptotic BCL2 family member. An analysis of sequence/structure of BCL2 family members allows us to define a structural superfamily, which has implications for general mechanisms for regulating proapoptotic activity. It appears two criteria must be met for proapoptotic function within the BCL2 family: targeting of molecules to intracellular membranes, and exposure of the BH3 death domain. BID's activity is regulated by a Caspase 8-mediated cleavage event, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, resulting in a change of cellular localization.  (+info)

Caspases induce cytochrome c release from mitochondria by activating cytosolic factors. (4/724)

We investigated the ability of caspases (cysteine proteases with aspartic acid specificity) to induce cytochrome c release from mitochondria. When Jurkat cells were induced to undergo apoptosis by Fas receptor ligation, cytochrome c was released from mitochondria, an event that was prevented by the caspase inhibitor, zVAD-fmk (zVal-Ala-Asp-CH2F). Purified caspase-8 triggered rapid cytochrome c release from isolated mitochondria in vitro. The effect was indirect, as the presence of cytosol was required, suggesting that caspase-8 cleaves and activates a cytosolic substrate, which in turn is able to induce cytochrome c release from mitochondria. The cytochrome c releasing activity was not blocked by caspase inhibition, but was antagonized by Bcl-2 or Bcl-xL. Caspase-8 and caspase-3 cleaved Bid, a proapoptotic Bcl-2 family member, which gains cytochrome c releasing activity in response to caspase cleavage. However, caspase-6 and caspase-7 did not cleave Bid, although they initiated cytochrome c release from mitochondria in the presence of cytosol. Thus, effector caspases may cleave and activate another cytosolic substrate (other than Bid), which then promotes cytochrome c release from mitochondria. Mitochondria significantly amplified the caspase-8 initiated DEVD-specific cleavage activity. Our data suggest that cytochrome c release, initiated by the action of caspases on a cytosolic substrates, may act to amplify a caspase cascade during apoptosis.  (+info)

Ion channel activity of the BH3 only Bcl-2 family member, BID. (5/724)

BID is a member of the BH3-only subgroup of Bcl-2 family proteins that displays pro-apoptotic activity. The NH(2)-terminal region of BID contains a caspase-8 (Casp-8) cleavage site and the cleaved form of BID translocates to mitochondrial membranes where it is a potent inducer of cytochrome c release. Secondary structure and fold predictions suggest that BID has a high degree of alpha-helical content and structural similarity to Bcl-X(L), which itself is highly similar to bacterial pore-forming toxins. Moreover, circular dichroism analysis confirmed a high alpha-helical content of BID. Amino-terminal truncated BIDDelta1-55, mimicking the Casp-8-cleaved molecule, formed channels in planar bilayers at neutral pH and in liposomes at acidic pH. In contrast, full-length BID displayed channel activity only at nonphysiological pH 4.0 (but not at neutral pH) in planar bilayers and failed to form channels in liposomes even under acidic conditions. On a single channel level, BIDDelta1-55 channels were voltage-gated and exhibited multiconductance behavior at neutral pH. When full-length BID was cleaved by Casp-8, it too demonstrated channel activity similar to that seen with BIDDelta1-55. Thus, BID appears to share structural and functional similarity with other Bcl-2 family proteins known to have channel-forming activity, but its activity exhibits a novel form of activation: proteolytic cleavage.  (+info)

A novel BH3-like domain in BID is required for intramolecular interaction and autoinhibition of pro-apoptotic activity. (6/724)

Upon activation of the Fas apoptotic signaling pathway, Bid, a "BH3 domain-only" pro-apoptotic molecule, is cleaved by caspase-8 into a 6.5-kDa N-terminal and a 15-kDa BH3 domain-containing C-terminal fragment, referred to as t(n)-Bid and t(c)-Bid, respectively. t(c)-Bid is a more potent inducer of apoptosis than full-length Bid, suggesting that the N-terminal region of Bid has an inhibitory effect on its pro-apoptotic activity. Here, we report the identification of a novel BH3-like motif (amino acid residues 35-43) in t(n)-Bid. Although Bid does not homodimerize, t(n)-Bid is able to associate avidly with t(c)-Bid. Site-directed mutagenesis revealed that both the novel BH3-like and BH3 domains are necessary for direct binding between t(n)-Bid and t(c)-Bid. While full-length Bid does not associate with t(n)-Bid, substitution of Leu(35), a critical residue in mediating t(n)-Bid/t(c)-Bid interaction, with Ala in full-length Bid is sufficient to establish Bid/t(n)-Bid interaction. Interestingly, the L35A Bid mutant is as effective as t(c)-Bid in inducing apoptosis and binding Bcl-X(L). We propose that the intramolecular interaction involving the BH3-like and BH3 domains serves to regulate the pro-apoptotic potential of Bid.  (+info)

The tyrosine kinase lck is required for CD95-independent caspase-8 activation and apoptosis in response to ionizing radiation. (7/724)

Induction of apoptosis is a hallmark of cytostatic drug and radiation-induced cell death in human lymphocytes and lymphoma cells. However, the mechanisms leading to apoptosis are not well understood. We provide evidence that ionizing radiation induces a rapid activation of caspase-8 (FLICE) followed by apoptosis independently of CD95 ligand/receptor interaction. The radiation induced cleavage pattern of procaspase-8 into mature caspase-8 resembled that following CD95 crosslinking and resulted in cleavage of the proapoptotic substrate BID. Overexpression of dominant-negative caspase-8 interfered with radiation-induced apoptosis. Caspase-8 activation by ionizing radiation was not observed in cells genetically defective for the Src-like tyrosine kinase Lck. Cells lacking Lck also displayed a marked resistance towards apoptosis induction upon ionizing radiation. After retransfection of Lck, caspase-8 activation and the capability to undergo apoptosis in response to ionizing radiation was restored. We conclude that radiation activates caspase-8 via an Lck-controlled pathway independently of CD95 ligand expression. This is a novel signaling event required for radiation induced apoptosis in T lymphoma cells.  (+info)

Evidence for a function of death-receptor-related, death-domain-containing proteins in anoikis. (8/724)

Normal epithelial cells undergo apoptosis if integrinmediated matrix contacts are lost, in a process termed 'anoikis'. Anoikis prevents shed epithelial cells from colonizing elsewhere, and is thus essential for maintaining appropriate tissue organisation. Aberrant oncogenes or tumor suppressor genes can cause resistance to anoikis, thereby contributing substantially to malignancy. Apoptosis is mediated by a well-ordered signaling cascade, which involves activation of intracellular proteases known as caspases. However, the mechanism by which the caspase cascade is initiated following cell-matrix detachment is unknown. We have hypothesized that death receptor activation might be involved in anoikis. To test this hypothesis, we developed a transient assay for anoikis and used it to assay the effects of proteins that block the function of domains found within death receptors known as death domains. In this assay, silencer of death domains (SODD) and dominant-negative FAS-associated death domain protein (FADD) efficiently inhibited anoikis in Madin-Darby canine kidney (MDCK) cells. The protective activity of SODD required its BAG domain, which interacts with the heat shock proteins hsp70 and hsc70, and inhibits the chaperone activity of the latter. Both caspase 8, which physically associates with death receptors, and cleavage of the caspase-8 substrate BID, were activated by cell-matrix detachment. These findings indicate a role for death receptors or proteins with related death domains in triggering anoikis.  (+info)

*Bufotalin

... cancer cell's susceptibility to apoptosis via TNF-α signalling by the BH3 interacting domain death agonist and STAT proteins. ... Waiwut, P; Inujima, A; Inoue, H; Saiki, I; Sakurai, H (January 2012). "Bufotalin sensitizes death receptor-induced apoptosis ...

*Phorbol-12-myristate-13-acetate-induced protein 1

Apoptosis Apoptosome Bcl-2 Bcl-2-associated X protein (BAX) BH3 interacting domain death agonist (BID) Caspases Cytochrome c ... Kim JY, Ahn HJ, Ryu JH, Suk K, Park JH (2004). "BH3-only protein Noxa is a mediator of hypoxic cell death induced by hypoxia- ... Phorbol-12-myristate-13-acetate-induced protein 1 is a protein that in humans is encoded by the PMAIP1 gene, and is also known ... until displaced by BH3-only proteins". Genes Dev. 19 (11): 1294-305. doi:10.1101/gad.1304105. PMC 1142553 . PMID 15901672. Oda ...

*P53 upregulated modulator of apoptosis

Apoptosis Apoptosome Bcl-2 Bcl-2-associated X protein (BAX) BH3 interacting domain death agonist (BID) Caspases Cytochrome c ... The PUMA protein is part of the BH3-only subgroup of Bcl-2 family proteins. This group of proteins only share sequence ... "Structure of the BH3 domains from the p53-inducible BH3-only proteins Noxa and Puma in complex with Mcl-1". J. Mol. Biol. 380 ( ... Webster KA (July 2006). "Puma joins the battery of BH3-only proteins that promote death and infarction during myocardial ...

*Bcl-2

Bcl-xL BH3 interacting domain death agonist (BID) Caspases Cytochrome c Noxa Mcl-1 Mitochondrion Microphthalmia-associated ... These pro-apoptotic proteins are in turn activated by BH3-only proteins, and are inhibited by the function of BCL-2 and its ... Hsu SY, Lin P, Hsueh AJ (Sep 1998). "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic ... This is the first FDA approval of a protein-protein inhibitor of BCL-2. Bcl-2 has been shown to interact with: BAK1, BCAP31, ...

*Myristoylation

The pro-apoptotic BH3-interacting domain death agonist (Bid) is one such protein that once myristoylated, translocates to the ... membrane-protein and protein-protein interactions. Further modifications on N-myristoylated proteins can add another level of ... Dual acylation of proteins can facilitate more tightly regulated protein localization, specifically targeting proteins to lipid ... Myristoylation allows for weak protein-protein and protein-lipid interactions and plays an essential role in membrane targeting ...

*Bcl-2-associated X protein

Apoptosis Apoptosome Bcl-2 BH3 interacting domain death agonist (BID) Caspases Cytochrome c Noxa Mitochondrion p53 upregulated ... binds the BH3 domain of other BAX or BCL-2 proteins in its active form. In the protein's inactive form, the groove binds its ... This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with ... domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers. These domains are composed of nine α-helices, with a ...

*Lipid-anchored protein

After the protein BH3 interacting-domain death agonist (Bid) has been myristoylated, it targets the protein to move to the ... but the site where the lipid binds to the protein depends both on the lipid group and protein. prenylated proteins are proteins ... This allows for the interaction of proteins with cellular membranes and protein domains. Overall, there are three main types of ... Lipid-anchored proteins (also known as lipid-linked proteins) are proteins located on the surface of the cell membrane that are ...

*List of MeSH codes (D12.644)

... bcl-x protein MeSH D12.644.360.075.718.968 --- bh3 interacting domain death agonist protein MeSH D12.644.360.100 --- ca(2+)- ... proto-oncogene proteins c-bcl-2 MeSH D12.644.360.075.718.100 --- bcl-associated death protein MeSH D12.644.360.075.718.400 --- ... rap gtp-binding proteins MeSH D12.644.360.525.475.100 --- rap1 gtp-binding proteins MeSH D12.644.360.525.500 --- ras proteins ... grb2 adaptor protein MeSH D12.644.360.024.298 --- grb7 adaptor protein MeSH D12.644.360.024.300 --- grb10 adaptor protein MeSH ...

*BH3 interacting-domain death agonist

The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family ... The BH3 interacting-domain death agonist has been shown to interact with: ATR/ATRIP, BCL2, CASP2, CASP8, MCL1, and RPA. Several ... BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with ... a novel BH3 domain-only death agonist". Genes Dev. 10 (22): 2859-69. doi:10.1101/gad.10.22.2859. PMID 8918887. Weinberg, Robert ...

*C6orf222

... as a pro-apoptotic protein due to the conserved BH3 interacting domain death agonist located at the C-terminus of the protein ... with the most extensive conservation being found at the C-terminus of the protein.The BH3 interacting-domain death agonist (BID ... The C6orf222 protein contains one mammalian conserved domain: DUF3293. The protein is also predicted to contain a BH3 domain, ... A predicted BH3 domain in the C6orf222 protein was found to interact with both Bcl-2 and Bcl-xL, implicating C6orf222 as being ...

*Caspase 2

... has been shown to interact with: BH3 interacting domain death agonist, CRADD, and Caspase 8. The Proteolysis Map ... a long pro-domain that is similar to that of caspase 9 and contains a protein interaction domain known as a CARD domain. Pro- ... Together with RAIDD and p53-induced protein with a death domain ([PIDD])(LRDD), caspase 2 has been shown to form the so-called ... including RIP-associated Ich-1/Ced-3-homologue protein with a death domain (RAIDD), apoptosis repressor with caspase ...

*File:Ideogram house mouse chromosome 6.svg

BH3 interacting-domain death agonist. *BRAF (gene). *Bone morphogenetic protein 10. *C-Met ... Public domainPublic domainfalsefalse This image is in the public domain because it contains materials that originally came from ... Email the source site if it is not clearly stated that this specific work is in the public domain. ... the NIH frequently uses commercial images which are not public domain. ...

*Photoactivated peptide

BH3 interacting-domain death agonist) Luis Moroder, G. Andrew Woolley, Rudolf K. Allemann, Jean Chmielewski N. Umezawa; Y. Noro ... "Reversible Photocontrol of DNA Binding by a Designed GCN4-bZIP Protein". Biochemistry. 45: 6075-6084. doi:10.1021/bi060142r. ... 2007). "Investigation into the P3 binding domain of m-calpain using photoswitchable diazo- and triazene-dipeptide aldehydes: ... CS1 maint: Multiple names: authors list (link) "Light triggers cancer death switch". KurzweilAI. doi:10.1039/C3MB70246D. ...

*List of MeSH codes (D12.776.476)

... bcl-x protein MeSH D12.776.476.075.718.937 -- bh3 interacting domain death agonist protein MeSH D12.776.476.150.300.100 -- ... bcl-associated death protein MeSH D12.776.476.075.718.400 -- bcl-2-associated x protein MeSH D12.776.476.075.718.425 -- bcl-2 ... smad1 protein MeSH D12.776.476.024.417.500.200 -- smad2 protein MeSH D12.776.476.024.417.500.300 -- smad3 protein MeSH D12.776. ... smad proteins, inhibitory MeSH D12.776.476.024.417.249.600 -- smad6 protein MeSH D12.776.476.024.417.249.700 -- smad7 protein ...

*Bcl-2-interacting killer

This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK. Bcl-2-interacting killer has been ... a BH3-containing mouse protein that interacts with Bcl-2 and Bcl-xL, is a potent death agonist". J. Biol. Chem. 273 (14): 7783- ... a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and ... Han J, Sabbatini P, White E (1996). "Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B ...

*BCL2L11

The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of ... 1996). "BID: a novel BH3 domain-only death agonist". Genes Dev. 10 (22): 2859-69. doi:10.1101/gad.10.22.2859. PMID 8918887. Zha ... Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with ... 2002). "Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis". Mol. ...

*Tyrosine-protein kinase BLK

1998). "Blk, a BH3-containing mouse protein that interacts with Bcl-2 and Bcl-xL, is a potent death agonist". J. Biol. Chem. ... a conserved nuclear phosphoprotein that contains multiple tetratricopeptide repeats and binds specifically to SH2 domains". J. ... The tyrosine-protein kinase BLK has been shown to interact with UBE3A. GRCh38: Ensembl release 89: ENSG00000136573 - Ensembl, ... 1993). "Mapping of sites on the Src family protein tyrosine kinases p55blk, p59fyn, and p56lyn which interact with the effector ...

*Bcl-2-associated death promoter

Identification of a BH-3 domain and analysis of its binding to mutant BCL-2 and BCL-XL proteins". J. Biol. Chem. 272 (49): ... encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)". EMBO ... Zha J, Harada H, Yang E, Jockel J, Korsmeyer SJ (1996). "Serine phosphorylation of death agonist BAD in response to survival ... "The proapoptotic BH3-only protein BAD transduces cell death signals independently of its interaction with Bcl-2". Cell Death ...

*Bcl-2 family

Milliman CL, Korsmeyer SJ, Wang K, Yin XM, Chao DT (1996). "BID: a novel BH3 domain-only death agonist". Genes Dev. 10 (22): ... The BH3-only subset of the Bcl-2 family of proteins contain only a single BH3-domain. The BH3-only members play a key role in ... Arbel, Nir; Shoshan-Barmatz, Varda (2010-02-26). "Voltage-dependent anion channel 1-based peptides interact with Bcl-2 to ... or those proteins that have only the BH3 domain (e.g. Bim Bid and BAD). All proteins belonging to the Bcl-2 family contain ...

*Programmed cell death

Autophagy Autoschizis Bcl-2 BH3 interacting domain death agonist (BID) Calpains Caspases Cell damage Cornification Cytochrome c ... Research on the corn poppy (Papaver rhoeas) has revealed that proteins in the pistil on which the pollen lands, interact with ... Programmed cell death (or PCD) is the death of a cell in any form, mediated by an intracellular program. PCD is carried out in ... Some categorize death in those organisms as unregulated apoptosis-like cell death. Biologists had long suspected that ...

*YWHAQ

Pierrat B, Ito M, Hinz W, Simonen M, Erdmann D, Chiesi M, Heim J (May 2000). "Uncoupling proteins 2 and 3 interact with members ... Zha J, Harada H, Yang E, Jockel J, Korsmeyer SJ (1997). "Serine phosphorylation of death agonist BAD in response to survival ... "Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function". Mol. ... This article incorporates text from the United States National Library of Medicine, which is in the public domain.. ...

*Apoptosis

... and Fas-associated death domain protein (FADD). cIAP1/2 can inhibit TNF-α signaling by binding to TRAF2. FLIP inhibits the ... Bax or Bak are activated by the activation of BH3-only proteins, part of the Bcl-2 family. ... Akt phosphorylates and inhibits Bas (a Bcl-2 family member), causing Bas to interact with the 14-3-3 scaffold, resulting in Bcl ... therefore overexpression leads to a decrease in the amount of proapoptotic agonists. As a consequence, the balance of anti- ...

*Apoptosis

... and Fas-associated death domain protein (FADD). cIAP1/2 can inhibit TNF-α signaling by binding to TRAF2. FLIP inhibits the ... Bax or Bak are activated by the activation of BH3-only proteins, part of the Bcl-2 family. ... Akt phosphorylates and inhibits Bad (a Bcl-2 family member), causing Bad to interact with the 14-3-3 scaffold, resulting in Bcl ... therefore overexpression leads to a decrease in the amount of proapoptotic agonists. As a consequence, the balance of anti- ...

*Apoptosis

... and Fas-associated death domain protein (FADD). cIAP1/2 can inhibit TNF-α signaling by binding to TRAF2. FLIP inhibits the ... Bax or Bak are activated by the activation of BH3-only proteins, part of the Bcl-2 family. Caspases Caspases play the central ... Akt phosphorylates and inhibits Bas (a Bcl-2 family member), causing Bas to interact with the 14-3-3 scaffold, resulting in Bcl ... therefore overexpression leads to a decrease in the amount of proapoptotic agonists. As a consequence, the balance of anti- ...
Chemical-registry-number] 0 / Antigens, CD95; 0 / BH3 Interacting Domain Death Agonist Protein; 0 / BID protein, human; 0 / Carrier Proteins; 9007-43-6 / Cytochromes c; EC 3.4.22.- / CASP3 protein, human; EC 3.4.22.- / CASP8 protein, human; EC 3.4.22.- / CASP9 protein, human; EC 3.4.22.- / Caspase 3; EC 3.4.22.- / Caspase 8; EC 3.4.22.- / Caspase 9; EC 3.4.22.- / ...
The mitochondrial carrier homologue-2 (MTCH2). Binds the BH3-interacting domain death agonist, BID. Regulated (induced) by the hepatocyte growth factor receptor, HGF/SF or Met. It has been proposed that its transport function has been lost (Robinson et al., 2012). Surprisingly, this protein has been reported to be targetted to the outer mitochondrial membrane (Gutiérrez-Aguilar and Baines, 2013 ...
Bid antibody (BH3 interacting domain death agonist) for ELISA, ICC/IF, IHC-P, WB. Anti-Bid pAb (GTX31689) is tested in Mouse samples. 100% Ab-Assurance.
Background The fundamental oil (EO) of L. particular caspase inhibitors showed that activation of caspase-8 was following and reliant towards the activation of caspases-9 and -3. In addition, the fundamental essential oil triggered a disruption from the mitochondrial transmembrane potential (m), improved the discharge of cytochrome towards the cytosol, and modified the manifestation of certain people of Bcl-2 family members (Bcl-2, Bax and Bet), Apaf-1 and XIAP. Oddly enough, low dosages of AVO-b and AVO-1 induced apoptosis in a variety of malignancy cellular lines also, however, not in noncancerous cellular material. Conclusions The full total outcomes demonstrate how the EO-induced apoptosis in HL-60 cellular material is definitely mediated by caspase-dependent pathways, concerning caspases-3, -9, and -8, that are initiated by Bcl-2/Bax/Bid-dependent lack of 173039-10-6 manufacture m resulting in launch of cytochrome towards the cytoplasm to activate the caspase cascade. The discovering that ...
Background The fundamental oil (EO) of L. particular caspase inhibitors showed that activation of caspase-8 was following and reliant towards the activation of caspases-9 and -3. In addition, the fundamental essential oil triggered a disruption from the mitochondrial transmembrane potential (m), improved the discharge of cytochrome towards the cytosol, and modified the manifestation of certain people of Bcl-2 family members (Bcl-2, Bax and Bet), Apaf-1 and XIAP. Oddly enough, low dosages of AVO-b and AVO-1 induced apoptosis in a variety of malignancy cellular lines also, however, not in noncancerous cellular material. Conclusions The full total outcomes demonstrate how the EO-induced apoptosis in HL-60 cellular material is definitely mediated by caspase-dependent pathways, concerning caspases-3, -9, and -8, that are initiated by Bcl-2/Bax/Bid-dependent lack of 173039-10-6 manufacture m resulting in launch of cytochrome towards the cytoplasm to activate the caspase cascade. The discovering that ...
PHP & tietojärjestelmäarkkitehtuuri Projects for $100. We are looking for someone to secure our backend control panel from SQL injection. Our system is built using PHP and OOP. Please only bid if you have extensive knowledge in PHP and OOP. We will have...
INTERNAL CANDIDATES: Please submit your bid by clicking on the APPLY button no later than 12:00 Noon, on the closing date. Complete ALL fields on the electronic bid form. Failure to complete the job bid may render you ineligible for this position. Please direct all questions to Human Resources: 814.234.6143. ...
Government Bid Opportunity: Z--Telecommunications cable, infrastructure installation. Access many more Z - Maintenance, Repair or Alteration of Real Property Bids | Get your Free Government Bids Alert!
A bid given to the balancing power market to increase generation or reduce consumption. When the bid is activated, the party carrying out the regulation sells regulating power to Fingrid.. ...
Government Pre-Bid Opportunity: K--Cadmium Plating. Access many more K - Modification of Equipment Upcoming Bids | Get your Free Government Bids Alert!
Hewitt Associates agreed to acquire Exult for about $691 million in stock, in a bid to expand its presence in the business-process-outsourcing market.
A blog about herpetology: snakes, lizards, frogs, salamanders, turtles, ecology, evolution, behavior, conservation, recent research, phylogeny.
Looking for online definition of apoptic death agonist in the Medical Dictionary? apoptic death agonist explanation free. What is apoptic death agonist? Meaning of apoptic death agonist medical term. What does apoptic death agonist mean?
Two types of cell death can be distinguished by morphological features, although it is likely that these are two ends of a spectrum with possible intermediate forms. Apoptosis involves shrinkage, nuclear disassembly, and fragmentation of the cell into discrete bodies with intact plasma membranes. These are rapidly phagocytosed by neighbouring cells. An important feature of apoptosis is the requirement for adenosine triphosphate (ATP) to initiate the execution phase. In contrast, necrotic cell death is characterized by cell swelling and lysis. This is usually a consequence of profound loss of mitochondrial function and resultant ATP depletion, leading to loss of ion homeostasis, including volume regulation, and increased Ca2+. The latter activates a number of nonspecific hydrolases (i.e., proteases, nucleases, and phospholipases) as well as calcium dependent kinases. Activation of calpain I, the Ca2+-dependent cysteine protease cleaves the death-promoting Bcl-2 family members Bid and Bax which ...
For having zero national title implications, Week 15 in the Big 12 is about as exciting as it can get. Three teams will have a chance to win the conference: Baylor, Oklahoma State and Texas...
Ethiopia. 5. Bidders should submit their offers accompanied by copies of all appropriate documents/license in a wax-sealed and stamped envelope to the above address into the box ready for the bid before 4:30 P.M. on April 28, 2006.. 6. Bidders shall furnish as part of their bid, bid security the amount of ETB 10,000 (Ten Thousand Birr) in the form of certified cheque (CPO) issued by a recognized bank, which will be refunded as soon as the first stock of production is received by GOAL Ethiopia as per the specification.. 7. Bid will be opened in the presence of bidders, their legal representatives who choose to attend on May 2, 2006 at 9:00 A.M. at the GOAL Ethiopia s meeting Room, Head Office, Addis Ababa.. 8. GOAL Ethiopia receives the right to accept or reject any or all bids.. GOAL Ethiopia ...
Klavunat Bid; zat rree, kulakta geli en enfeksiyonlar, bron it, sin zit, idrar yollar iltihab ve cilt enfeksiyonlar n n tedavisinde etkilidir.
Head and neck squamous cell carcinomas (HNSCC) induced by human papilloma virus (HPV) have increased recently in the US, and exhibit a different prognosis and response to therapies from HPV(-) cancers. Analysis of HNSCC TCGA datasets provide evidence for distinct alterations in expression of components of the NF-κB and death pathways in HNSCC with different HPV status. Previously, we have found that birinipant, a novel SMAC mimetic that inhibits inhibitor of apoptosis proteins (IAPs), sensitizes a subset of HPV(-) HNSCC cell lines to death agonists like TNF-α and TRAIL. In this study, we have observed that birinipant also sensitizes most HPV(+) cell lines to TRAIL and TNF-α in vitro. The IC50 of birinipant was under 50nM for HPV(+) UPCI-SCC-90 and UM-SCC-47 cell lines, when combined with TNF-α or TRAIL. To explore the therapeutic potential of enhancing TRAILR mediated death signaling in HPV(+) HNSCC cells, we investigated the effects of an agonistic polyclonal TRAILR2 antibody. Treatment of ...
YC-137 is a BCL-2 inhibitor, which selectively induces apoptosis of Bcl-2-overexpressing cells and disrupts its interaction with Bid BH3, thereby blocking the anti-apoptotic activity of Bcl-2.
Im 31 weeks and this weekend have started to notice a pattern in BH. I get them from 8-10 every morning and theyre actually pretty painful. I thought I was going to have to call the doc yesterday as they were timeable and not letting up. This is my3rd and I dont remember this with the other 2. Any other ladies experience this?
Recommended Review Article:. Chipuk JE, Green DR. 2008. How do BCL-2 proteins induce mitochondrial outer membrane permeabilization? TRENDS IN CELL BIOLOGY 18(4): 157-164. Recommended Articles:. Aslan JE, Thomas G. 2009. Death by Committee: Organellar Trafficking and Communication in Apoptosis. TRAFFIC 10(10): 1390-1404. Chipuk JE, Green DR. 2009. PUMA cooperates with direct activator proteins to promote mitochondrial outer membrane permeabilization and apoptosis. CELL CYCLE 8(17): 2692-2696. Chipuk JE, Bouchier-Hayes L, Green DR. 2006. Mitochondrial outer membrane permeabilization during apoptosis: the innocent bystander scenario. CELL DEATH AND DIFFERENTIATION 13(8): 1396-1402 Chipuk JE, Green DR. 2006. Dissecting p53-dependent apoptosis. CELL DEATH AND DIFFERENTIATION 13(6): 994-1002 Chipuk JE, Bouchier-Hayes L, Kuwana T, et al. 2005. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53 . SCIENCE 309(5741): 1732-1735. Kuwana T, Bouchier-Hayes L, Chipuk JE, et al. 2005. BH3 ...
The proapoptotic BAX protein triggers apoptosis via the intrinsic pathway by inducing mitochondrial outer membrane permeabilization (MOMP). BAX largely exists in an inactive conformation in the cytoplasm, but under cellular stress BAX is activated by BH3-only proteins and translocates to the mitochondrial outer membrane to induce MOMP. Structural studies have revealed conformational changes at the N-terminal surface and C-terminal α9 helix that are required for BAX activation by BH3 proteins and MOMP induction, but suggest that additional mechanisms may stabilize BAX in the inactive cytosolic conformation. Garner, Reyna, and colleagues identified an autoinhibited dimeric BAX conformation in addition to the inactive monomer conformation. The BAX dimers did not induce membrane permeabilization, and, in contrast to BAX monomers, were resistant to BH3-mediated activation. Moreover, BAX dimers failed to translocate to the membrane upon BH3-induced stimulation. Crystallization studies indicated that ...
Important Notice: For ITBs over $50K, all prospective bidders must fax and email companys general information. This includes: the company name, address, phone number, fax number and email addresses, to the responsible Buyer before downloading the bid document.. Please note that: Addendums are NOT posted to the City website. Bidders must be identified as a current Vendor and on the official Plan Holders List in order to receive an addendum or revision to plans or specifications. Failure to contact this office with this pertinent information will cause a bid to be non-responsive and the bid may be rejected.. Full information regarding each individual bid can be accessed by clicking on the file numbers below. Some files will include additional documentation within the bid information, all additional information will be found at the bottom of the page and can be accessed by clicking on the provided link.. ...
Important Notice: For ITBs over $50K, all prospective bidders must fax and email companys general information. This includes: the company name, address, phone number, fax number and email addresses, to the responsible Buyer before downloading the bid document.. Please note that: Addendums are NOT posted to the City website. Bidders must be identified as a current Vendor and on the official Plan Holders List in order to receive an addendum or revision to plans or specifications. Failure to contact this office with this pertinent information will cause a bid to be non-responsive and the bid may be rejected.. Full information regarding each individual bid can be accessed by clicking on the file numbers below. Some files will include additional documentation within the bid information, all additional information will be found at the bottom of the page and can be accessed by clicking on the provided link.. ...
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An electronic person-to-person double auction-like system that facilitates trade in many differs sorts of item via the World Wide Web or similar such interface. A plurality of sellers can post details of items for sale, including a minimum bid amount and buy price for each. A buyer can create a bid basket comprising bids in respect of a plurality of heterogeneous items available for sale, together with bid-withdrawal rules defining circumstances in which respective bid(s) are to be withdrawn. Bids are binding unless withdrawn. The auction for any item continues until a buyer posts the buy price therefor or the seller accepts a bid posted therefor.
The SPCA has begun uplifting hordes of animals, birds and cats from a Northland farm after their owner lost a bid to appeal against
Verizons top executive on Tuesday left open the possibility that the US telecom giant could make a bid for Yahoo if the Internet firm puts itself up for sale.
id":"9119","bid":"30504","uid":"0","title":"dam","username":"Nikki1808","rating":"5","pros":"hmm","cons":"alrite","description":"its an alrite place ive heard..but check it out see fer yourself","date_added":"1207013128","date_modified":null,"is_external":"1","external_site_name":"zootoo","external_site_url":null,"external_user_profile":null,"status":"1"},{"id":"9118","bid":"30504","uid":"0","title":"One of the best","username":"J2C","rating":"5","pros":"Very smart","cons":"none","description":"a very great vet that you should take a visit to","date_added":"1202579490","date_modified":null,"is_external":"1","external_site_name":"zootoo","external_site_url":null,"external_user_profile":null,"status":"1 ...
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Recommended Review Article:. Chipuk JE, Green DR. 2008. How do BCL-2 proteins induce mitochondrial outer membrane permeabilization? TRENDS IN CELL BIOLOGY 18(4): 157-164. Recommended Articles:. Aslan JE, Thomas G. 2009. Death by Committee: Organellar Trafficking and Communication in Apoptosis. TRAFFIC 10(10): 1390-1404. Chipuk JE, Green DR. 2009. PUMA cooperates with direct activator proteins to promote mitochondrial outer membrane permeabilization and apoptosis. CELL CYCLE 8(17): 2692-2696. Chipuk JE, Bouchier-Hayes L, Green DR. 2006. Mitochondrial outer membrane permeabilization during apoptosis: the innocent bystander scenario. CELL DEATH AND DIFFERENTIATION 13(8): 1396-1402 Chipuk JE, Green DR. 2006. Dissecting p53-dependent apoptosis. CELL DEATH AND DIFFERENTIATION 13(6): 994-1002 Chipuk JE, Bouchier-Hayes L, Kuwana T, et al. 2005. PUMA couples the nuclear and cytoplasmic proapoptotic function of p53 . SCIENCE 309(5741): 1732-1735. Kuwana T, Bouchier-Hayes L, Chipuk JE, et al. 2005. BH3 ...
Our lab is cross-disciplinary, bringing together computational biology, protein design, and molecular biology approaches. We assess the structural bioinformatics of OMPs (outer membrane proteins) and apply the results to de novo OMP protein design and to native OMP manipulation.. OMPs are a ripe target for cancer therapeutics. Mitochondria have recently become a focus of cancer therapies due to the fact that mitochondrial outer membrane permeabilization leads to apoptosis or necrosis. We explore mitochondrial membrane permeabilization through manipulation of the OMP pores that already exist in the mitochondrial outer membrane. This may have pharmaceutical consequences because tumorigenic mitochondrial membranes can be selectively targeted in themselves as they have been shown to accumulate lipophilic cations.. Beyond this mechanistic understanding, knowledge of the relationship between OMP chemistry and structure will allow new OMPs to be designed for use in vaccines and will facilitate ...
Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with RTL10/BOP. Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and ...
The neighborhood itself has struggled to attract private businesses and office workers, in part because it lacks nearby services and retail. Service and retail providers in turn have had little incentive to open shop in Anacostia until more businesses and consumers are on the street. That conundrum is reflected in the embryonic BID formation. While corporations typically sponsor the BIDs that promote doing business in the neighborhood, that lack of commerce has hindered the Anacostia BIDs formation. Though landowners in the area point to much potential - a historic downtown, a Metro stop, the coming St. Elizabeths project - without a corporate base to fund the BID, lack of money has kept Anacostias promoter-in-chief from becoming a reality. That may be starting to change ...
The chairman of one of Chinas leading real estate investment groups Beijing Zhongkun, made famous by his $8.8 million bid to buy a 115 square mile farm in Iceland, says the deal would have been very profitable had it gone through.
Japans Dainippon Sumitomo Pharma Co Ltd(4506.T) plans to launch a bid to acquire Sepracor Inc SEPR.Ofor about 250 billion yen ($2.70 billion), the Nikkei businessdaily said.
Trade is continuing to be largely steady among the Beltex here at Carlisle, but with some of the better pens coming in now some good prices have been achie
Begrippenlijst Spoorse Begrippen bedoeld voor taalexamen voor machinist, rangeerder, wagencontroleur en treindienstleider Bronvermelding: Business Information Document van ProRail, documentnummer BID00009,
Apoptosis is a genetically programmed process for the elimination of damaged or redundant cells by activation of caspases (aspartate-specific cysteine proteases). The onset of apoptosis is controlled by numerous interrelating processes. The extrinsic pathway involves stimulation of members of the tumor necrosis factor (TNF) receptor subfamily, such as TNFRI, CD95/Fas or TRAILR (death receptors), located at the cell surface, by their specific ligands, such as TNF-alpha, FasL or TRAIL, respectively. The intrinsic pathway is activated mainly by non-receptor stimuli, such as DNA damage, ER stress, metabolic stress, UV radiation or growth-factor deprivation. The central event in the intrinsic pathway is the mitochondrial outer membrane permeabilization (MOMP), which leads to the release of cytochrome c. These two pathways converge at the level of effector caspases, such as caspase-3 and caspase-7. The third major pathway is initiated by the constituents of cytotoxic granules (e.g. Perforin and ...
The human lymphocyte toxins granzyme B (hGrzB) and perforin cooperatively induce apoptosis of virus-infected or transformed cells: perforin pores enable entry of the serine protease hGrzB into the cytosol, where it processes Bid to selectively activate the intrinsic apoptosis pathway. Truncated Bid (tBid) induces Bax/Bak-dependent mitochondrial outer membrane permeability and the release of cytochrome c and Smac/Diablo. To identify cellular proteins that regulate perforin/hGrzB-mediated Bid cleavage and subsequent apoptosis, we performed a gene-knockdown (KD) screen using a lentiviral pool of short hairpin RNAs embedded within a miR30 backbone (shRNAmiR ...
Downloadable! This paper presents the theory of sequential auctions when participants desire more than one unit. The theoretical predictions are compared to experimental data. We estimate structural models which presume that participants bids are drawn from some stochastic processes. The observed behavior depends highly on the theoretical bid, however some variance in participant bids remains unexplained. The analysis of the model and of the experimental data is completed by some simulations. While the theoretical model predicts increasing prices, on average both the data and the winning bids generated by our structural models exhibit declining prices. Ce travail concerne la théorie des enchères séquentielles où les participants peuvent acheter plusieurs unités dun bien homogène. Les prédictions théoriques des modèles sont comparées à des données expérimentales. Nous estimons des modèles structuraux qui supposent que les mises des participants sont tirées dun processus stochastique. Le
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Now that the bids are all in and we await the outcome, the Patients for LICH want to thank the staff of Long Island College Hospital (LICH). This past years fight to save LICH has brought together a coalition of healthcare workers, nurses, doctors, community groups, patients, and elected officials. However, you are the ones who demonstrated tremendous courage, dedication and grace each and every day as you reported for work to care for your patients under the most stressful of working conditions. Many of you report to work under the threat of being laid off.
A method of identifying an individual from examination of their natural physical characteristics is shown. The method comprises obtaining from an individual during a registration process, a fingerprint image having at least one registration pore and at least one registration macrofeature; wherein registration pore data is derived from the registration pores, and registration macrofeature data is derived from the registration macrofeatures. In a bid step, a fingerprint image having at least one bid pore and at least one bid macrofeature is obtained; wherein bid pore data is derived from the bid pores and bid macrofeature data is derived from the bid macrofeatures. Bid associated data is constructed from associating the bid pore data with the bid macrofeature data, and constructing registration associated data derived from associating the registration pore data with the registration macrofeature data. The bid associated data is compared to the registration associated data to produce a correlation score
Bid opening date May 07, 2008 at 11:00 A.M.. Bids will be opened in the presence of bidders or their representatives on the Specified date and time here above.. The company has the right to accept or reject any or all of the bids.. Wonji/Shoa Sugar Factory ...
The Los Angeles BID Wiki will eventually collect and organize all of our information and understanding of BIDs in Los Angeles. Sadly this project is on hold due to time constraints. If youre interested in collaborating on it, you can drop me a line ...
From the Adams Morgan BID: Adams Morgan Partnership BID office was broken into over the weekend. Our office, along with several other offices in the Festival Center, was broken into over the holiday weekend. We are offering a $1k no-questions-asked reward for the return of the two Dell laptops if they are returned in
The purpose of this study is to to assess the effect of ATV 300 mg BID on the PK of raltegravir 400 mg BID, to assess the effect of raltegravir 400 mg BID on the PK of ATV 300 mg BID, and to assess the ECG effects of ATV 300 mg BID over 21 days, given with or without raltegravir 400 mg BID ...
The purpose of this study is to to assess the effect of ATV 300 mg BID on the PK of raltegravir 400 mg BID, to assess the effect of raltegravir 400 mg BID on the PK of ATV 300 mg BID, and to assess the ECG effects of ATV 300 mg BID over 21 days, given with or without raltegravir 400 mg BID ...
This seminar explored how to detect and prevent bid rigging in public procurement using the OECD Guidelines for Fighting Bid Rigging. A variety of case studies in which procurement officials detected bid rigging or designed procedures for preventing bid rigging will be discussed. Key focal areas for the seminar are investigative best practices and techniques, leniency programs, trade associations, and the use of indirect evidence. ...
Turkey is showing renewed interest in reviving its stalled bid to join the European Union, now that one of its key opponents is no longer the president of France.
Malaysian conglomerateDRB-Hicom Bhd on Tuesday said it is waiting forprospective foreign carmakers to submit bids for a strategicpartnership with its Proton Holdings Bhd unit.
Zlatan Ibrahimovic could become the most expensive player in history after Paris Saint-Germain bid for the AC Milan striker. The French giants submitted a joint offer of £51million for Ibrahimovic and Thiago Silva.
id":"9706","bid":"32581","uid":"0","title":"Good time","username":"BabyBlueDad","rating":"4","pros":"dog loved his visit","cons":"got muddy while we were there","description":"We visited a couple times, the dog loved it. Lots of new friends to meet.","date_added":"1236578502","date_modified":null,"is_external":"1","external_site_name":"zootoo","external_site_url":null,"external_user_profile":null,"status":"1"},{"id":"9705","bid":"32581","uid":"0","title":"Good","username":"ajswanny","rating":"5","pros":"Good","cons":"None","description":"Love it. There are two seperate enclosures, and the people that you see there are always very friendly.","date_added":"1235513829","date_modified":null,"is_external":"1","external_site_name":"zootoo","external_site_url":null,"external_user_profile":null,"status":"1"},{"id":"9704","bid":"32581","uid":"0","title":"Enjoyable dog park!","username":"Daneroo","rating":"4","pros":"Split for small or large dogs.","cons":"Sometimes muddy.","description":"My dog always ...
After apparently considering takeover offers all day, directors of People Express adjourned late Tuesday without taking any action.Deliberations will resume Wednesday morning, said a spokeswoman
The president of Barre-based Milne Travel says hes considering challenging Gov. Peter Shumlin this fall. I am thinking seriously of a run but will not...
Page contains details about two-dimensional (BA)2SnI3(BH4) . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Apoptosis is a genetically programmed process for the elimination of damaged or redundant cells by activation of caspases (aspartate-specific cysteine proteases). The onset of apoptosis is controlled by numerous interrelating processes. The extrinsic pathway involves stimulation of members of the tumor necrosis factor (TNF) receptor subfamily, such as TNFRI, CD95/Fas or TRAILR (death receptors), located at the cell surface, by their specific ligands, such as TNF-alpha, FasL or TRAIL, respectively. The intrinsic pathway is activated mainly by non-receptor stimuli, such as DNA damage, ER stress, metabolic stress, UV radiation or growth-factor deprivation. The central event in the intrinsic pathway is the mitochondrial outer membrane permeabilization (MOMP), which leads to the release of cytochrome c. These two pathways converge at the level of effector caspases, such as caspase-3 and caspase-7. The third major pathway is initiated by the constituents of cytotoxic granules (e.g. Perforin and ...
Today, theres no viable "con" position in the "paid search bid management" debate. Either you sing the praises of automated bid management, or youll be asked to sit in a dark corner (or cave) and contemplate what possessed you to enter the digital marketing arena. Unfortunately, there are few real debates on the advantages and disadvantages of different approaches to bid management automation. This is mainly because most of the dialogue - be it on a panel, in online content, or in casual conversation - is driven by bid management software vendors. So, in this article, Ill try to open a dialogue to address this often one-sided debate. Ill begin by highlighting some of the (often incorrect) fundamental assumptions underlying current bid management tools. In future columns Ill address additional aspects of the debate.. Large or complex campaign - that is the question. In the bid management space, there are 50 or so bid management vendors, but only a handful of good ones. For marketers trying ...
If youre sitting on the edge of your seat waiting and hoping for a major Warriors sale development before Thursdays draft…. Better scoot back down. Relax. This does not appear like it will be in any way an abbreviated process, which, given the parameters and players involved, is not surprising.. From the beginning I was told late-July would be the earliest to realistically look for a deal closing and mid-August might be a much better target.. Now: Guess what, it looks like late-July will be the earliest we could possibly hear about an agreement and mid-August seems like a more likely target.. What I hear is that all the parties are still deep in the examination process-taking long, careful looks at the Warriors finances-and that no binding bids have been been made or asked for.. Auctioneer Sal Galatioto might be weeks away from seeking formal bids. And once the binding bids are in, there will almost certainly be further negotiations-a final round, so to speak.. So the draft is not a key ...
The USDAs Farm Service Agency has made a second solicitation under the Feedstock Flexibility Program for Bioenergy Producers. Approximately 830 million pounds of sugar are being offered for sale under the program. Bids are due Sept. 26.
A consortium led by General Electric submitted the only bid for a Nigerian railway concession project worth around $2 billion for two lines connecting northern cities to others in the south, a procurement process adviser said on Wednesday. Nigeria has been looking for partners to overhaul its ageing
Chandanasava Saraswatharista- mix these two preparation, dose is 15ml twice daily up to the age of 12, above 12 yrs of age 20 ml twice daily T. Agnitundi vati- 50mg tablet should be given twice daily. This dose is from age 5 to 18. T. Chandraprabha vati 250mg half bid till age of 12 later 1 bid can be given. Here we can also consider many other preparation as per the need. But in Ukkinadkas Ayurveda this is the best acting combination in use, where we can expect result within 3 months. We have many such cases that got relieved of symptoms within 3 months and in some needed treatment for more than 6 months ...
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Government bids, RFPs and contracts are gathered by GovDirections and classified by state contract and delivered via a daily email alert to registered contractors.
On reflection, looking back at 2017, it would be fair to say that I took a massive risk, and placed a cardiac arrest bid on a black horse, before the horse could enter the Inner Circle. Do I have regrets investing in people who did not deliver expectations or maybe did not even show up when it was their turn to show up? The truth is that I would not change a single step of the way that brought me where I am today, four days before 2017 is closing its doors. Each time I placed a bid this year, I did not expect anyone else to enter the Inner Circle, so others could SEE the horse in the Inner Circle and place a bid on that horse. I am the black horse. I place the bid on me. I know that if a big dream visits me, I already have everything I need to turn it into a vision I all I have to do is to focus on what I have, forget what I dont have. It is unfair to wait for the perfect moment to come for me. I take this present moment and turn it into a perfect moment. without worrying what others would ...
The Los Angeles BID Wiki will eventually collect and organize all of our information and understanding of BIDs in Los Angeles. Sadly this project is on hold due to time constraints. If youre interested in collaborating on it, you can drop me a line ...
BASFs proposed acquisition of fish oil specialist Pronova BioPharma is almost done and dusted now that over 97% of the latters stockholders have tendered their shares. - News - PharmaTimes
(AP)-Irish drug maker Elan Corp. announced Monday it will pay shareholders a recurring special dividend linked to the sales of the multiple sclerosis treatment Tysabri.
I heard about this one. Apparently, she was to be medically verified to be a virgin yet, Ive been informed that such a state can not be verified by anyone. I dont think there are many people who dont roll their eyes at this one. The bozos who bid need their heads examined too. ...
Is there any link between prostatitis and high blood pressure? Since Ive had this horrible thing, my blood pressure has been rising slowly. Could this...login to view the rest of this post ...
Birth Weight and Systolic Blood Pressure in Adolescence and Adulthood: Meta-Regression Analysis of Sex- and Age-specific Results from 20 Nordic Studies ...
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Read "The Pro-Apoptotic Proteins, Bid and Bax, Cause a Limited Permeabilization of the Mitochondrial Outer Membrane That Is Enhanced by Cytosol, The Journal of Cell Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Los Angeles, Cal., Tuesday, May 27, 1947 Nisbt Pbooe: RI. 5472 NO. 143 LaPorte Tells Many Worries Of Graduation Commencement Head Now In 32nd Year As Ritual Counselor Seniors Warned To Buy Tickets For Ball Soon "Tickets for thc senior ball are selling: fast. With only 500 bids available for 2000 students, seniors who are determined to hear Les Brown at the Riviera Beach club Junl 14, better get their bids right away," emphasized Manuel Mireles, publicist for the event, yesterday. "Weve picked our chaperones and made final arrangements •with Les Brown." continued Mireles, "and are just waiting for the remaining bids to sell before packing up until June 14." Bids are on sale at the cashiers office. Fall Preregistration Fixed for Returnees The ball site, again explained Mireles, is the Riviera Beach club and not the country club of the same name. It is located in south Los Angeles, off Sepulveda boule-avrd. toward Redondo Beach. Dancing is from 9 p.m. to 1 a.m. Plebes result of what the ...
Apoptosis is the physiological process used by an organism to selectively eliminate cells that are no longer needed, have been damaged or are dangerous ( Kerr et al., 1972). This process, critical for sculpting organs during development and ensuring homeostasis throughout life, has been conserved during evolution ( Vaux and Strasser, 1996). Defects in the control of apoptosis have been implicated as a cause or a contributing factor in a variety of diseases. For example, abnormal survival of cells that should be killed can cause cancer ( Strasser et al., 1990) or autoimmune disease ( Bouillet et al., 1999; Strasser et al., 1991b; Watanabe-Fukunaga et al., 1992), whereas premature death of normally long-lived cells may be the cause of certain degenerative disorders ( Barr and Tomei, 1994).. Genetic and biochemical studies have identified two major pathways to programmed cell death that are largely independent ( Strasser et al., 1995). On the one hand, apoptosis can be triggered by ligation of a ...
The first steps are un-der way in a bid to bring the 2018 Com-mon-wealth Games to Auck-land.. Of-fi-cers from lo-cal coun-cils are un-der-tak-ing pre-lim-i-nary work with key or-gan-i-sa-tions in the re-gion, be-fore a $325,000 fea-si-bil-ity study, co-funded by cen-tral and lo-cal govern-ment, be-gins next month.. Events Knowl-edge Ser-vice, a con-sul-tant to the Com-mon-wealth Games Fed-er-a-tion, will use the in-for-ma-tion gath-ered to de-ter-mine whether Auck-land is ca-pa-ble of host-ing the event, with a re-port due in Septem-ber.. Auck-land city mayor John Banks says he knows the ben-e-fits an event like the Com-mon-wealth Games can bring, be-cause he was Sports Min-is-ter in 1990 when the games were last held in Auck-land.. "The in-ter-na-tional bo-nanza that year show-cased the very best of Auck-land and New Zealand with huge eco-nomic ben-e-fits.. "The 2018 games will be al-most three times as big and will get three times as many eco-nomic ben-e-fits.". Mr Banks says a re-de-vel-oped ...
ATLANTA -- US Airways increased the pressure on Delta Air Lines unsecured creditors to take its buyout offer by raising the bid Wednesday nearly 20 percent to $10.3 billion. Whether the deal would survive regulatory scrutiny may be the deciding factor.
There are 343 comments on the USA Today story from Jul 22, 2014, titled Bachmann says she may make another presidential bid. In it, USA Today reports that: Rep. Michele Bachmann says she is considering a run for president in 2016. The Minnesota Republican told Real Clear Politics on Tuesday that she learned a great deal from her 2012 campaign.. Join the discussion below, or Read more at USA Today.. ...
In his new role as US president, Donald Trump puts US interests above everything else - even above long-established alliances. In a bid to unite the nation Trump shoots for nationalism, says DWs Ines Pohl in Washington.
Los Angeles Angels-bound Shohei Ohtani bid farewell to fans of his former Japanese club on Monday as he sets off to join his new Major League Baseball team.
MOUNT WASHINGTON-When building a $7 million sports park, problems are expected.. But having not contractors interest was not one Land Design and Development manager Chad McCormick was expecting.. "We were very concerned that we only received one bid given the amount of interest we received," McCormick told the city council.. The bid opening on July 6 left those involved in the project befuddled as the only proposal that came in was a $10,500,000 bid from Martin Construction Company. ...
That the whistle for 2019 has long been blown is not in doubt. While many of the people in the (now rejuvenated?) PDP with rumoured presidential ambitions seem too scared to throw their hats into the ring,
Hedge fund Eminence Capital, which owns a 9.8 percent stake in MW, followed with a filing with the SEC seeking from MW shareholders a
Unable to secure the support of a single parliamentary colleague Alan Duncan has abandoned his bid to be Tory leader. His bid has been generously covered by the media class and in an article for The Guardian Mr Duncan records...
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J Biol Chem. 2012 Apr 27;287(18):15016-23. doi: 10.1074/jbc.M111.328377. Epub 2012 Mar 13. Research Support, Non-U.S. Govt; Research Support, U.S. Govt, Non-P.H.S.
Functional interaction of Bcl-2/Bcl-xL and Bax/Bak, but not Bid/Bik, with the VDAC. Bax/Bak directly opens the VDAC to induce cytochrome c release, while Bcl-2/
Korhonen, L., Belluardo, N., Mudo, G. and Lindholm, D. (2003), Increase in Bcl-2 phosphorylation and reduced levels of BH3-only Bcl-2 family proteins in kainic acid-mediated neuronal death in the rat brain. European Journal of Neuroscience, 18: 1121-1134. doi: 10.1046/j.1460-9568.2003.02826.x ...
Two Brooklyn hospitals, SUNY Downstate Medical Center and Long Island College Hospital (LICH), are on the agenda of the March board meetings of the State University of New York (SUNY), taking place Tuesday, March 18th and Wednesday, March 19th. SUNY is in the process of selling LICH, located in Cobble Hill, through a reissued Request for Proposals (RFP) process worked out after a year-long legal battle. Final bids are due Wednesday, March 19.
ANCHORAGE, Alaska (AP) - Oil companies this week had the chance to bid on 900 tracts within Alaskas National Petroleum Reserve but offered bids on just...
The District government is releasing its request for proposals for a long-term ground lease for a city-owned lot at the corner of Florida and Sherman Avenues. The solicitation could go live as early as today or Monday, a city official told DCMud (Update, since original publication of this article the city has published the RFP). While one developer reportedly already has detailed plans for the site, the open bid puts one of the Districts more visible sites up for bid in a neighborhood where developers are already planning extensive construction ...
MOUNT PLEASANT, S.C. (AP) - Former South Carolina Gov. Mark Sanford on Tuesday cleared another hurdle in his bid for political redemption, defeating a former Charleston County council member to win the GOP nomination for the U.S. House seat he held for three terms.
Youd think by now Id stop, but I cant. Finding deals is like crack to me. Its addictive and gives me this rush like WEEEEEE! IM FLYIIIIIIING! But somewhere in the back of my head I say to myself, "You know, you know you dont REALLY need this (yes you do crazy lady its only $10 for a f***ing bridle, shut up you cheap-o) and I have like 20 pairs of boots already (BITCH, its comes with the bridle, shut up! and bid on it already!!!!) and I dont get paid till next friday and rent is due and thats like $400 (BID ON IT BID ON IT BID ON IT)....its like a horrible scene with smegel from LOTR ...
Visits to Temple Street childrens hospital in Dublin are being limited to parents and guardians in a bid to protect patients…
By Norihiko Shirouzu and Deepa Seetharaman BEIJING/DETROIT, March 28 (Reuters) - Chinese state-owned carmaker Dongfeng Motor Group will not pursue a bid for a majority stake in Fisker Automotive, according to sources familiar with the matter, leaving the troubled U.S. electric car without a clear financial backer.
Cardiologist Wouter Bassons bid for a discharge on four charges of unprofessional and unethical conduct has failed, but a hearing will be held later in the year.
TY - JOUR. T1 - Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. AU - Luo, Xu. AU - Budihardjo, Imawati. AU - Zou, Hua. AU - Slaughter, Clive. AU - Wang, Xiaodong. PY - 1998/8/21. Y1 - 1998/8/21. N2 - We report here the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as Fas and TNF. Peptide mass fingerprinting identified this protein as Bid, a BH3 domain- containing protein known to interact with both Bcl2 and Bax. Caspase-8 cleaves Bid, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Immunodepletion of Bid from cell extracts eliminated the cytochrome c releasing activity. The cytochrome c releasing activity of Bid was antagonized by Bcl2. A mutation at the BH3 domain diminished its cytochrome c releasing activity. Bid, ...

Gentaur Molecular :Prospecbio \ Recombinant Mouse BH3 Interacting Domain Death Agonist GST RECOMBINANT & NATURAL PROTEINS \ pro...Gentaur Molecular :Prospecbio \ Recombinant Mouse BH3 Interacting Domain Death Agonist GST RECOMBINANT & NATURAL PROTEINS \ pro...

NATURAL PROTEINS \ pro-643 for more molecular products just contact us ... Recombinant Mouse BH3 Interacting Domain Death Agonist GST RECOMBINANT & ... NATURAL PROTEINS mus musculus murine Recombinant Mouse BH3 Interacting Domain Death Agonist GST RECOMBINANT & NATURAL PROTEINS ... NATURAL PROTEINS. Related products : Recombinant Mouse BH3 Interacting Domain Death Agonist GST RECOMBINANT & NATURAL PROTEINS ...
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JCI -
Targeting the extrinsic apoptotic pathway in cancer: lessons learned and future directionsJCI - Targeting the extrinsic apoptotic pathway in cancer: lessons learned and future directions

BH3-interacting domain death agonist; cFLIP, cellular FLICE-inhibitory protein; CUL3, cullin 3; DISC, death-inducing signaling ... Wiezorek J, Holland P, Graves J. Death receptor agonists as a targeted therapy for cancer. Clin Cancer Res. 2010;16(6):1701- ... Opposing unfolded-protein-response signals converge on death receptor 5 to control apoptosis. Science. 2014;345(6192):98-101. ... Triggering cell death: The crystal structure of Apo2L/TRAIL in a complex with death receptor 5. Mol Cell. 1999;4(4):563-571. ...
more infohttps://www.jci.org/articles/view/80420

Frontiers | BID Mediates Oxygen-Glucose Deprivation-Induced Neuronal Injury in Organotypic Hippocampal Slice Cultures and...Frontiers | BID Mediates Oxygen-Glucose Deprivation-Induced Neuronal Injury in Organotypic Hippocampal Slice Cultures and...

... is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been ... is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been ... The BH3 interacting-domain death agonist (BID) is a pro-apoptotic protein involved in death receptor-induced and mitochondria- ... The BH3 interacting-domain death agonist (BID) is a proapoptotic B-cell lymphoma-2 (BCL-2) family protein involved in death ...
more infohttps://www.frontiersin.org/articles/10.3389/fncel.2016.00014/full

Targeted Therapy-based Combination Treatment in Rhabdomyosarcoma | Molecular Cancer TherapeuticsTargeted Therapy-based Combination Treatment in Rhabdomyosarcoma | Molecular Cancer Therapeutics

... receptor-interacting serine/threonine-protein kinase 1; BID, BH3 interacting-domain death agonist; BAX, Bcl-2-associated X ... Fas-associated protein with death domain; CASP, caspase; RIP1, ... RIP1 protein-dependent assembly of a cytosolic cell death ... Similar to IGF1R, FGFR4 is a transcriptional target of the PAX3-FOXO1 fusion protein leading to increased protein expression ( ... In this regard, targeting of RTKs, downstream proteins, Hedgehog signaling, DDR, cell-cycle proteins and the epigenome shows ...
more infohttp://mct.aacrjournals.org/content/17/7/1365.full

Role of Cathepsin S in Periodontal Inflammation and InfectionRole of Cathepsin S in Periodontal Inflammation and Infection

BH3 interacting domain death agonist (BID); BCL2 antagonist killer 1 (BAK1); tumor necrosis factor (ligand) superfamily, member ... transmembrane protein 74 (TMEM74); death-associated protein kinase 1 (DAPK1); eukaryotic translation initiation factor 4 gamma ... Figure 2: (a) Effects of IL-1β (1 ng/mL) or F. nucleatum (OD: 0.025) on CTSS protein level in supernatants at 1 d and 2 d, as ... The changes in CTSS gene expression by IL-1β and F. nucleatum were further studied at protein level. As analyzed by ELISA, F. ...
more infohttps://www.hindawi.com/journals/mi/2017/4786170/

TCDB » SEARCHTCDB » SEARCH

The mitochondrial carrier homologue-2 (MTCH2). Binds the BH3-interacting domain death agonist, BID. Regulated (induced) by the ... It has been proposed that its transport function has been lost (Robinson et al., 2012). Surprisingly, this protein has been ...
more infohttp://www.tcdb.org/search/result.php?tc=2.A.29.25.2

Expi293 Protein Expression Data | Thermo Fisher Scientific - USExpi293 Protein Expression Data | Thermo Fisher Scientific - US

... which incorporates a GST tag to the N-terminal of each protein. The proteins were expressed by transfection of the cloned ... BH3 interacting domain death agonist (BID), transcript variant 2, mRNA. NM_001196.3. NP_001187.1. 972. ... Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B, mRNA (cDNA clone MGC:18122 IMAGE: ... BCL2-associated agonist of cell death (BAD), transcript variant 1, mRNA. NM_004322.3. NP_004313.1. 22. ...
more infohttps://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-expression/mammalian-protein-expression/transient-mammalian-protein-expression/expi293-expression-system/expi293-protein-expression-data.html

Protein alkylation leading to liver fibrosis (Homo sapiens) - WikiPathwaysProtein alkylation leading to liver fibrosis (Homo sapiens) - WikiPathways

BH3-interacting domain death agonist. Protein. P55957 (Uniprot-TrEMBL) Biglycan. Protein. P21810 (Uniprot-TrEMBL) CCL2. ... KE1: Cell injury/death (KE55). Histone H2AX. Cytochrome C. AIF1. BH3-interacting domain death agonist. BAX. Calpain-1 catalytic ... Protein. E9PRM1 (Uniprot-TrEMBL) Caspase-3. Protein. A8MVM1 (Uniprot-TrEMBL) Caspase-9. Protein. F8VWA5 (Uniprot-TrEMBL) ... Protein alkylation. Collagen accumulation. KER 269. MIE: Protein alkylation (KE244). Stressors. Proto-oncogene c-Rel. Toll-like ...
more infohttps://www.wikipathways.org/index.php?title=Pathway:WP3980&oldid=92320

BID (human)BID (human)

Home > Protein > BID human. mouse. rat. New Protein Search:. BH3-interacting domain death agonist ... Protein Mutation Frequency in Cancer. The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA ...
more infohttps://www.phosphosite.org/proteinAction.action?id=1421&showAllSites=true

Differentiation of the Mammary Epithelial Cell during Involution: Implications for Breast Cancer | Springer for Research &...Differentiation of the Mammary Epithelial Cell during Involution: Implications for Breast Cancer | Springer for Research &...

BH3 interacting domain death agonist. BMDM. bone marrow-derived macrophages. BMEC. bovine mammary epithelial cells ... MIR-interacting saposin-like protein or canopy 2 homolog or transmembrane protein 4 ... coiled-coil, moesin-like BCL2 interacting protein or autophagy related 6 homolog ... lectin, galactoside-binding, soluble, 3 or IgE-binding protein or laminin-binding protein ...
more infohttps://rd.springer.com/article/10.1007/s10911-009-9121-0

Phosphodiesterase 5 Inhibitors Enhance Chemotherapy Killing in Gastrointestinal/Genitourinary Cancer Cells | Molecular...Phosphodiesterase 5 Inhibitors Enhance Chemotherapy Killing in Gastrointestinal/Genitourinary Cancer Cells | Molecular...

BID, BH3 interacting-domain death agonist.. Prior studies have shown that activation of CD95 can stimulate the induction of LC3 ... or the linker protein Fas-associated death domain protein expression suppressed the toxicity enhancing activity of sildenafil ( ... Knock down of CD95 or Fas-associated death domain protein suppressed drug combination toxicity. Combination toxicity was also ... or siRNA molecules to knock down expression of CD95 or Fas-associated death domain protein (FADD) (siCD95, siFADD). Thirty-six ...
more infohttp://molpharm.aspetjournals.org/content/85/3/408?ijkey=f820bfe82a0e305ef17220f8eba6f3bfbff397ae&keytype2=tf_ipsecsha

BioLegend - Search ResultsBioLegend - Search Results

BH3 domain-only protein, BH3 interacting domain death agonist * 645001 Size 25 µg ... Phospho-Proteins (11) Postsynaptic proteins (25) Proteases (18) Protein Kinases/Phosphatase (42) Protein Modifiers (4) Siglec ... Protein Misfolding and Aggregation (418) Protein Synthesis (66) Protein Trafficking and Clearance (253) Signal Transduction ( ... Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1 ...
more infohttps://biolegend.com/ja-jp/search-results?GroupID=&PageNum=2&view=detail&Category=PRIM_AB&BiologyArea=apoptosis_tumor_suppressors_cell_death

BioLegend - Search ResultsBioLegend - Search Results

BH3 domain-only protein, BH3 interacting domain death agonist * 645001 Size 25 µg ... Phospho-Proteins (13) Postsynaptic proteins (25) Proteases (18) Protein Kinases/Phosphatase (39) Protein Modifiers (4) Siglec ... Protein Misfolding and Aggregation (391) Protein Synthesis (64) Protein Trafficking and Clearance (233) Signal Transduction ( ... Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1 ...
more infohttps://www.biolegend.com/nl-be/search-results?GroupID=&PageNum=2&view=detail&Category=PRIM_AB&BiologyArea=apoptosis_tumor_suppressors_cell_death

A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics | Molecular & Cellular ProteomicsA Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics | Molecular & Cellular Proteomics

b, antibody HPR000722 raised against the 22-kDa BH3-interacting domain death agonist (BID) transcribed from chromosome 22. c, ... Protein Atlas for Cancer Tissues-. The protein atlas also contains a summary page for the protein profiles found in the 20 ... Altogether more than 700 proteins were analyzed representing all major types of protein families, i.e. protein receptors, ... The protein may play a role in central nervous system development by interacting with growth factors implicated in motor neuron ...
more infohttp://www.mcponline.org/content/4/12/1920

Frontiers | Differential Protein Profiling of Cerebrospinal Fluid in Piglets with Severe Meningoencephalitis Caused by...Frontiers | Differential Protein Profiling of Cerebrospinal Fluid in Piglets with Severe Meningoencephalitis Caused by...

A total of 813 differential proteins, including 52 up-regulated proteins and 761 down-regulated proteins, were found in the CSF ... A total of 813 differential proteins, including 52 up-regulated proteins and 761 down-regulated proteins, were found in the CSF ... The most enriched subclasses of upregulated proteins were extracellular (62.1%), protein metabolism (34.5%) and cysteine-type ... The most enriched subclasses of upregulated proteins were extracellular (62.1%), protein metabolism (34.5%) and cysteine-type ...
more infohttps://www.frontiersin.org/articles/10.3389/fcimb.2018.00035/full

Association between BH3 interacting domain death agonist (BID) gene polymorphism and ossification of the posterior longitudinal...Association between BH3 interacting domain death agonist (BID) gene polymorphism and ossification of the posterior longitudinal...

... of the BH3 interacting domain death agonist ( BID) gene as a risk factor in Korean patients with ossification of... ... Wang H, Yang ZH, Liu DM et al (2008) Association between two polymorphisms of the bone morpho-genetic protein-2 gene with ... Association between BH3 interacting domain death agonist (BID) gene polymorphism and ossification of the posterior longitudinal ... The purpose of this study was to investigate single nucleotide polymorphisms (SNPs) of the BH3 interacting domain death agonist ...
more infohttps://link.springer.com/article/10.1007/s11033-013-2933-4

Tumor immunity Wuhan USCN Business Co., Ltd.Tumor immunity Wuhan USCN Business Co., Ltd.

ELISA Kit DIY Materials for BH3 Interacting Domain Death Agonist (Bid). Product No.: KSA629Hu01. Organism species: Homo sapiens ... ELISA Kit DIY Materials for S100 Calcium Binding Protein A8 (S100A8). Product No.: KSB792Mu01. Organism species: Mus musculus ( ... ELISA Kit DIY Materials for BH3 Interacting Domain Death Agonist (Bid). Product No.: KSA629Ra01. Organism species: Rattus ... ELISA Kit DIY Materials for C Reactive Protein (CRP). Product No.: KSA821Ra01. Organism species: Rattus norvegicus (Rat). ...
more infohttp://uscnk.com/tags/Tumor%20immunity_34_132_All

TCDB » SEARCHTCDB » SEARCH

The mitochondrial carrier homologue-2 (MTCH2). Binds the BH3-interacting domain death agonist, BID. Regulated (induced) by the ... Domain. Kingdom/Phylum. Protein(s). *2.A.29.1.1. Mitochondrial ATP/ADP antiporter (SLC25A5) facilitates exchange of ADP and ATP ... has a 500 residue N-terminal hydrophilic domain as well as a C-terminal 100 residue hydrophilic domain. Both domains are ... The mitochondrial presenilin-associated protein (PSAP; MTCH1) binds to the PDZ domain (a QFYI motif) C-terminus of presenilin. ...
more infohttp://tcdb.org/search/result.php?tc=2.A.29.7

Bid BondBid Bond

The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family ... BH3 interacting-domain death agonist. The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the ... BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with ... BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with ...
more infohttps://wn.com/Bid_Bond?upload_time=all_time&orderby=published

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The mitochondrial carrier homologue-2 (MTCH2). Binds the BH3-interacting domain death agonist, BID. Regulated (induced) by the ... Domain. Kingdom/Phylum. Protein(s). *2.A.29.1.1. Mitochondrial ATP/ADP antiporter (SLC25A5) facilitates exchange of ADP and ATP ... has a 500 residue N-terminal hydrophilic domain as well as a C-terminal 100 residue hydrophilic domain. Both domains are ... The mitochondrial presenilin-associated protein (PSAP; MTCH1) binds to the PDZ domain (a QFYI motif) C-terminus of presenilin. ...
more infohttp://tcdb.org/search/result.php?tc=2.A.29.23

Bid Bond AgencyBid Bond Agency

The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family ... BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with ... This page contains text from Wikipedia, the Free Encyclopedia - https://wn.com/BH3_interacting-domain_death_agonist ... domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers. Bcl-2 proteins act as anti- or pro-apoptotic ...
more infohttps://wn.com/Bid_bond_agency?from=bidbondagency.net

anti-Bid antibody  | GeneTexanti-Bid antibody | GeneTex

BH3 interacting domain death agonist) for ELISA, ICC/IF, IHC-P, WB. Anti-Bid pAb (GTX31689) is tested in Mouse samples. 100% Ab ... BH3 interacting domain death agonist. Background. This gene encodes a death agonist that heterodimerizes with either agonist ... The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced ... Cancer > Apoptosis > Induction > Death domain receptors. *Cancer > Apoptosis > Metastasis and Invasion > Metastasis > Lymphatic ...
more infohttp://www.genetex.com/Bid-antibody-GTX31689.html

Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis...Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis...

rab7 protein * rab5 GTP-Binding Proteins * BH3 Interacting Domain Death Agonist Protein ... We report that expression of canonical BH3-only proteins, tBid, BimEL, Bik, Bad, and mitophagy receptor mutants of atypical BH3 ... We report that expression of canonical BH3-only proteins, tBid, BimEL, Bik, Bad, and mitophagy receptor mutants of atypical BH3 ... We report that expression of canonical BH3-only proteins, tBid, BimEL, Bik, Bad, and mitophagy receptor mutants of atypical BH3 ...
more infohttps://jhu.pure.elsevier.com/en/publications/intramitochondrial-recruitment-of-endolysosomes-mediates-smac-deg-3

Bufotalin - WikipediaBufotalin - Wikipedia

... cancer cells susceptibility to apoptosis via TNF-α signalling by the BH3 interacting domain death agonist and STAT proteins. ... Waiwut, P; Inujima, A; Inoue, H; Saiki, I; Sakurai, H (January 2012). "Bufotalin sensitizes death receptor-induced apoptosis ...
more infohttps://en.wikipedia.org/wiki/Bufotalin

Apoptosis and survival - Ceramides signaling pathway Pathway Map - PrimePCR | Life Science | Bio-RadApoptosis and survival - Ceramides signaling pathway Pathway Map - PrimePCR | Life Science | Bio-Rad

Ceramides via Cathepsin D/ BH3 interacting domain death agonist (Bid)/ Cytochrome c mediates TNF-alpha induced apoptosis. ... require TNFRSF1A-associated via death domain (TRADD)/ Fas (TNFRSF6)-associated via death domain (FADD) adaptor proteins [5], [6 ... This prevents BCL2-associated agonist of cell death (BAD) phosphorylation and leads to apoptosis in COS-7 cells. The mechanism ... Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta- ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/pathway/apoptosis-survival/apoptosis-survival-ceramides-signaling-pathway
  • The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
  • In pancreatic tumor cells expressing mutant active K-RAS, PDE5 inhibitors interacted in a greater than additive fashion with doxorubicin/gemcitabine/paclitaxel to cause cell death. (aspetjournals.org)
  • Koga H, Hayashi K, Taketomi E et al (1996) Restriction fragment length polymorphism of genes of the alpha 2(XI) collagen, bone morphogenetic protein-2, alkaline phosphatase, and tumor necrosis factor-alpha among patients with ossification of posterior longitudinal ligament and controls from the Japanese population. (springer.com)
  • My team developed a recombinant soluble version of the human ligand comprising the extracellular domain of the endogenous protein and possessing a homotrimeric structure. (jci.org)
  • This inhibition depends on protein phosphatase 2 ( PP2A )-mediated dephosphorylation and by blocking AKT(PKB) translocation to plasma membrane via Protein kinase C zeta ( PKC-zeta ). (bio-rad.com)
  • Expression of cellular FLICE-like inhibitory protein-short did not significantly inhibit chemotherapy lethality but did significantly reduce enhanced killing in combination with sildenafil. (aspetjournals.org)
  • Here we report a new publicly available database containing, in the first version, ∼400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. (mcponline.org)
  • In addition, specific antibodies directed to a particular target protein allow numerous functional assays to be performed ranging from conventional ELISA assays to detailed localization studies using fluorescent probes and protein capture experiments ("pull-down") for purification of specific proteins and their associated complexes for structural and biochemical analyses ( 4 , 5 ). (mcponline.org)
  • 9 ) showed that this strategy could be further improved by a streamlined approach for affinity purification of the antibodies to generate monospecific antibodies (msAbs) 1 and the subsequent validation of the specificity of these antibodies by protein microarrays. (mcponline.org)
  • Both "in-house" generated monospecific antibodies and antibodies from commercial sources were used for the profiling of a large number of protein targets. (mcponline.org)
  • Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. (mcponline.org)
  • The challenge for antibody-based proteomics is to move from a conventional protein-by-protein approach into a high throughput mode to allow chromosome wide analysis ( 6 , 7 ). (mcponline.org)
  • CSF was collected from infected piglets, and protein profiling was performed with label-free proteomics technology. (frontiersin.org)
  • The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BID. (wikipedia.org)
  • It is also possible to generate protein expression data on many different individual patients to evaluate heterogeneity of tissue profiles ( 2 , 3 ). (mcponline.org)
  • We show that a comprehensive atlas of human protein expression patterns in normal and cancer tissues can be created by combining the methods mentioned above. (mcponline.org)
  • In vivo , gingival tissue biopsies from rats with ligature-induced periodontitis and from periodontitis patients were also analyzed at transcriptional and protein levels. (hindawi.com)
  • The use of tissue microarrays (TMAs) generated from multiple biopsies combined into single paraffin blocks enabled high throughput analysis of protein expression in various tissues and organs ( 2 ). (mcponline.org)
  • 10 ) showed that high throughput analysis of protein expression can be performed with a standard set of tissue microarrays representing both normal and cancer tissues. (mcponline.org)
  • We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. (mcponline.org)
  • Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research. (mcponline.org)
  • The results are presented as a publicly available protein atlas database, and the data suggest that it should be possible to extend this analysis to most or all human proteins. (mcponline.org)
  • Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. (mcponline.org)
  • The present studies determined whether clinically relevant phosphodiesterase 5 (PDE5) inhibitors interacted with clinically relevant chemotherapies to kill gastrointestinal/genitourinary cancer cells. (aspetjournals.org)
  • In bladder cancer cells, regardless of H-RAS mutational status, at clinically achievable doses, PDE5 inhibitors interacted in a greater than additive fashion with doxorubicin/mitomycin C/gemcitabine/cisplatin/paclitaxel to cause cell death. (aspetjournals.org)