Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.
An alpha-glucosidase inhibitor with antiviral action. Derivatives of deoxynojirimycin may have anti-HIV activity.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
Diseases caused by the loss of one or more enzymes involved in the hydrolysis of mannoside linkages (MANNOSIDASES). The defects in enzyme activity are primarily associated with genetic mutation of the genes that codes for a particular mannosidase isoenzyme.
Five-carbon furanose sugars in which the OXYGEN is replaced by a NITROGEN atom.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
The characteristic 3-dimensional shape of a carbohydrate.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.
Established cell cultures that have the potential to propagate indefinitely.
A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
The rate dynamics in chemical or physical systems.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
An organic mercurial used as a sulfhydryl reagent.
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The N-acetyl derivative of glucosamine.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Proteins prepared by recombinant DNA technology.
A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Enzymes that catalyze the transfer of galactose from a nucleoside diphosphate galactose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
Also known as CD104 antigen, this protein is distinguished from other beta integrins by its relatively long cytoplasmic domain (approximately 1000 amino acids vs. approximately 50). Five alternatively spliced isoforms have been described.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
Integrin beta chains combine with integrin alpha chains to form heterodimeric cell surface receptors. Integrins have traditionally been classified into functional groups based on the identity of one of three beta chains present in the heterodimer. The beta chain is necessary and sufficient for integrin-dependent signaling. Its short cytoplasmic tail contains sequences critical for inside-out signaling.
Polysaccharides consisting of mannose units.
A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
A partitioning within cells due to the selectively permeable membranes which enclose each of the separate parts, e.g., mitochondria, lysosomes, etc.
An N-acetylglycosamine containing antiviral antibiotic obtained from Streptomyces lysosuperificus. It is also active against some bacteria and fungi, because it inhibits the glucosylation of proteins. Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms.
The process of cleaving a chemical compound by the addition of a molecule of water.
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). The adrenergic beta-2 receptors are more sensitive to EPINEPHRINE than to NOREPINEPHRINE and have a high affinity for the agonist TERBUTALINE. They are widespread, with clinically important roles in SKELETAL MUSCLE; LIVER; and vascular, bronchial, gastrointestinal, and genitourinary SMOOTH MUSCLE.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.
A group of alicyclic hydrocarbons with the general formula R-C5H9.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Nonionic surfactant mixtures varying in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups. They are used as detergents, emulsifiers, wetting agents, defoaming agents, etc. Octoxynol-9, the compound with 9 repeating ethoxy groups, is a spermatocide.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
Glycoproteins found on the membrane or surface of cells.
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). The adrenergic beta-1 receptors are equally sensitive to EPINEPHRINE and NOREPINEPHRINE and bind the agonist DOBUTAMINE and the antagonist METOPROLOL with high affinity. They are found in the HEART, juxtaglomerular cells, and in the central and peripheral nervous systems.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A glycogen synthase kinase that was originally described as a key enzyme involved in glycogen metabolism. It regulates a diverse array of functions such as CELL DIVISION, microtubule function and APOPTOSIS.
The sum of the weight of all the atoms in a molecule.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
One of the ESTROGEN RECEPTORS that has greater affinity for ISOFLAVONES than ESTROGEN RECEPTOR ALPHA does. There is great sequence homology with ER alpha in the DNA-binding domain but not in the ligand binding and hinge domains.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). The beta-3 adrenergic receptors are the predominant beta-adrenergic receptor type expressed in white and brown ADIPOCYTES and are involved in modulating ENERGY METABOLISM and THERMOGENESIS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Adherence of cells to surfaces or to other cells.
Brain waves with frequency between 15-30 Hz seen on EEG during wakefulness and mental activity.
Drugs that selectively bind to and activate beta-adrenergic receptors.
ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 2.7.7.7.
Compounds bind to and activate ADRENERGIC BETA-2 RECEPTORS.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
Cell-surface proteins that bind transforming growth factor beta and trigger changes influencing the behavior of cells. Two types of transforming growth factor receptors have been recognized. They differ in affinity for different members of the transforming growth factor beta family and in cellular mechanisms of action.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
AMINO ALCOHOLS containing the propanolamine (NH2CH2CHOHCH2) group and its derivatives.
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
Nucleocytoplasmic transport molecules that bind to ALPHA KARYOPHERINS in the CYTOSOL and are involved in transport of molecules through the NUCLEAR PORE COMPLEX. Once inside the CELL NUCLEUS beta karyopherins interact with RAN GTP-BINDING PROTEIN and dissociate from alpha karyopherins. Beta karyopherins bound to RAN GTP-BINDING PROTEIN are then re-transported to the cytoplasm where hydrolysis of the GTP of RAN GTP-BINDING PROTEIN causes release of karyopherin beta.
A phosphoinositide phospholipase C subtype that is primarily regulated by its association with HETEROTRIMERIC G-PROTEINS. It is structurally related to PHOSPHOLIPASE C DELTA with the addition of C-terminal extension of 400 residues.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Drugs that bind to but do not activate beta-adrenergic receptors thereby blocking the actions of beta-adrenergic agonists. Adrenergic beta-antagonists are used for treatment of hypertension, cardiac arrhythmias, angina pectoris, glaucoma, migraine headaches, and anxiety.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Compounds that bind to and activate ADRENERGIC BETA-3 RECEPTORS.
A forkhead transcription factor that regulates expression of metabolic GENES and is involved in EMBRYONIC DEVELOPMENT. Mutations in HNF-3beta have been associated with CONGENITAL HYPERINSULINISM.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
An integrin that binds to a variety of plasma and extracellular matrix proteins containing the conserved RGD amino acid sequence and modulates cell adhesion. Integrin alphavbeta3 is highly expressed in OSTEOCLASTS where it may play role in BONE RESORPTION. It is also abundant in vascular smooth muscle and endothelial cells, and in some tumor cells, where it is involved in angiogenesis and cell migration. Although often referred to as the vitronectin receptor there is more than one receptor for vitronectin (RECEPTORS, VITRONECTIN).
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.
One of the two major classes of cholinergic receptors. Nicotinic receptors were originally distinguished by their preference for NICOTINE over MUSCARINE. They are generally divided into muscle-type and neuronal-type (previously ganglionic) based on pharmacology, and subunit composition of the receptors.

Molecular cloning, sequencing, and expression of a novel multidomain mannanase gene from Thermoanaerobacterium polysaccharolyticum. (1/115)

The manA gene of Thermoanaerobacterium polysaccharolyticum was cloned in Escherichia coli. The open reading frame of manA is composed of 3,291 bases and codes for a preprotein of 1,097 amino acids with an estimated molecular mass of 119,627 Da. The start codon is preceded by a strong putative ribosome binding site (TAAGGCGGTG) and a putative -35 (TTCGC) and -10 (TAAAAT) promoter sequence. The ManA of T. polysaccharolyticum is a modular protein. Sequence comparison and biochemical analyses demonstrate the presence of an N-terminal leader peptide, and three other domains in the following order: a putative mannanase-cellulase catalytic domain, cellulose binding domains 1 (CBD1) and CBD2, and a surface-layer-like protein region (SLH-1, SLH-2, and SLH-3). The CBD domains show no sequence homology to any cellulose binding domain yet reported, hence suggesting a novel CBD. The duplicated CBDs, which lack a disulfide bridge, exhibit 69% identity, and their deletion resulted in both failure to bind to cellulose and an apparent loss of carboxymethyl cellulase and mannanase activities. At the C-terminal region of the gene are three repeats of 59, 67, and 56 amino acids which are homologous to conserved sequences found in the S-layer-associated regions within the xylanases and cellulases of thermophilic members of the Bacillus-Clostridium cluster. The ManA of T. polysaccharolyticum, besides being an extremely active enzyme, is the only mannanase gene cloned which shows this domain structure.  (+info)

Cloning and sequencing of beta-mannosidase gene from Aspergillus aculeatus no. F-50. (2/115)

The manB gene, coding for a unique beta-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli beta-galactosidase and the human beta-guclonidase was conserved. This is the first fungal gene that encodes MANB.  (+info)

Mannan-degrading enzymes from Cellulomonas fimi. (3/115)

The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and beta-mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian beta-mannosidases.  (+info)

A family 26 mannanase produced by Clostridium thermocellum as a component of the cellulosome contains a domain which is conserved in mannanases from anaerobic fungi. (4/115)

Cellulosomes prepared by the cellulose affinity digestion method from Clostridium thermocellum culture supernatant hydrolysed carob galactomannan during incubation at 60 degrees C and pH 6.5. A recombinant phage expressing mannanase activity was isolated from a library of C. thermocellum genomic DNA constructed in lambdaZAPII. The cloned fragment of DNA containing a putative mannanase gene (manA) was sequenced, revealing an ORF of 1767 nt, encoding a protein (mannanase A; Man26A) of 589 aa with a molecular mass of 66816 Da. The putative catalytic domain (CD) of Man26A, identified by gene sectioning and sequence comparisons, displayed up to 32% identity with other mannanases belonging to family 26. Immediately downstream of the CD and separated from it by a short proline/threonine linker was a duplicated 24-residue dockerin motif, which is conserved in all C. thermocellum cellulosomal enzymes described thus far and mediates their attachment to the cellulosome-integrating protein (CipA). Man26A consisting of the CD alone (Man26A") was hyperexpressed in Escherichia coli BL21(DE3) and purified. The truncated enzyme hydrolysed soluble and insoluble mannan, displaying a temperature optimum of 65 degrees C and a pH optimum of 6.5, but exhibited no activity against other plant cell wall polysaccharides. Antiserum raised against Man26A" cross-reacted with a polypeptide with a molecular mass of 70000 Da that is part of the C. thermocellum cellulosome. A second variant of Man26A containing the N-terminal segment of 130 residues and the CD (Man26A") bound to ivory-nut mannan and weakly to soluble Carob galactomannan and insoluble cellulose. Man26A" consisting of the CD alone did not bind to these polysaccharides. These results indicate that the N-terminal 130 residues of mature Man26A may constitute a weak mannan-binding domain. Sequence comparisons revealed a lack of identity between this region of Man26A and other polysaccharide-binding domains, but significant identity with a region conserved in the three family 26 mannanases from the anaerobic fungus Piromyces equi.  (+info)

The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA. (5/115)

A five-gene cluster around the gene in Clostridium cellulovorans that encodes endoglucanase EngL, which is involved in plant cell wall degradation, has been cloned and sequenced. As a result, a mannanase gene, manA, has been found downstream of engL. The manA gene consists of an open reading frame with 1,275 nucleotides encoding a protein with 425 amino acids and a molecular weight of 47, 156. ManA has a signal peptide followed by a duplicated sequence (DS, or dockerin) at its N terminus and a catalytic domain which belongs to family 5 of the glycosyl hydrolases and shows high sequence similarity with fungal mannanases, such as Agaricus bisporus Cel4 (17.3% identity), Aspergillus aculeatus Man1 (23.7% identity), and Trichoderma reesei Man1 (22.7% identity). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and N-terminal amino acid sequence analyses of the purified recombinant ManA (rManA) indicated that the N-terminal region of the rManA contained a DS and was truncated in Escherichia coli cells. Furthermore, Western blot analysis indicated that ManA is one of the cellulosomal subunits. ManA production is repressed by cellobiose.  (+info)

A gene encoding a novel multidomain beta-1,4-mannanase from Caldibacillus cellulovorans and action of the recombinant enzyme on kraft pulp. (6/115)

Genomic walking PCR was used to obtained a 4,567-bp nucleotide sequence from Caldibacillus cellulovorans. Analysis of this sequence revealed that there were three open reading frames, designated ORF1, ORF2, and ORF3. Incomplete ORF1 encoded a putative C-terminal cellulose-binding domain (CBD) homologous to members of CBD family IIIb, while putative ORF3 encoded a protein of unknown function. The putative ManA protein encoded by complete manA ORF2 was an enzyme with a novel multidomain structure and was composed of four domains in the following order: a putative N-terminal domain (D1) of unknown function, an internal CBD (D2), a beta-mannanase catalytic domain (D3), and a C-terminal CBD (D4). All four domains were linked via proline-threonine-rich peptides. Both of the CBDs exhibited sequence similarity to family IIIb CBDs, while the mannanase catalytic domain exhibited homology to the family 5 glycosyl hydrolases. The purified recombinant enzyme ManAd3 expressed from the cloned catalytic domain (D3) exhibited optimum activity at 85 degrees C and pH 6.0 and was extremely thermostable at 70 degrees C. This enzyme exhibited high specificity with the substituted galactomannan locust bean gum, while more substituted galacto- and glucomannans were poorly hydrolyzed. Preliminary studies to determine the effect of the recombinant ManAd3 and a recombinant thermostable beta-xylanase on oxygen-delignified Pinus radiata kraft pulp revealed that there was an increase in the brightness of the bleached pulp.  (+info)

Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei. (7/115)

The enzymatic degradation of single crystals of mannan I with the catalytic core domain of a beta-mannanase (EC 3.2.1.78 or Man5A) from Trichoderma reesei was investigated by transmission electron microscopy and electron diffraction. The enzyme attack took place at the edge of the crystals and progressed towards their centres. Quite remarkably the crystalline integrity of the crystals was preserved almost to the end of the digestion process. This behaviour is consistent with an endo-mechanism, where the enzyme interacts with the accessible mannan chains located at the crystal periphery and cleaves one mannan molecule at a time. The endo mode of digestion of the crystals was confirmed by an analysis of the soluble degradation products.  (+info)

Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening. (8/115)

Activity of endo-beta-mannanase increases during ripening of tomato (Lycopersicon esculentum Mill.) fruit of the cultivar Trust. beta-Mannoside mannohydrolase is also present during ripening, but its pattern of activity is different from that of endo-beta-mannanase. The increase in endo-beta-mannanase activity is greatest in the skin, and less in the outer and inner pericarp regions. This enzyme is probably bound to the walls of the outermost cell layers of the fruit during ripening, and it requires a high-salt buffer for effective extraction. The enzyme protein, as detected immunologically on Western blots, is present during the early stages of ripening, before any enzyme activity is detectable. The mRNA for the enzyme is also present at these stages; endo-beta-mannanase may be produced and sequestered in a mature-sized inactive form during early ripening. Most non-ripening mutants of tomato exhibit reduced softening and lower endo-beta-mannanase activity, but a cause-and-effect relationship between the enzyme and ripening is unlikely because some cultivars which ripen normally do not exhibit any endo-beta-mannanase activity in the fruit.  (+info)

A recent study in Genetics in Medicine aiming at the identification by whole-exome sequencing of genes involved in infantile nystagmus found heterozygous missense mutations in the MANBA gene, which the encodes lysosomal beta-mannosidase. The mutations resulted in decrease of ?-mannosidase activities in the patients as well as in mutant-transfected HEK293T cells. MANBA is expressed in the pretectal nucleus of the developing midbrain, […]. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis
Pathogen or diet-induced immune activation can partition energy and nutrients away from growth, but clear relationships between immune responses and the direction and magnitude of energy partitioning responses have yet to be elucidated. The objectives were to determine how β-mannanase supplementation and lipopolysaccharide (LPS) immune stimulation affect maintenance energy requirements (MEm) and to characterize immune parameters, digestibility, growth performance, and energy balance. In a randomized complete block design, 30 young weaned pigs were assigned to either the control treatment (CON; basal corn, soybean meal and soybean hulls diet), the enzyme treatment (ENZ; basal diet + 0.056% β-mannanase), or the immune system stimulation treatment (ISS; basal diet + 0.056% β-mannanase, challenged with repeated increasing doses of Escherichia coli LPS). The experiment consisted of a 10-d adaptation period, 5-d digestibility and nitrogen balance measurement, 22 h of heat production (HP) measurements, and
The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow measurement of activity over a 175 degrees C temperature range, from +90 degrees C to -85 degrees C for the glutamate dehydrogenase and from +90 degrees C to -70 degrees C for the beta-glucosidase. The Arrhenius plots of these enzymes, and those for two mesophilic enzymes (glutamate dehydrogenase from bovine liver and beta-galactosidase from Escherichia coli), exhibit no downward deflection corresponding to the glass transition, found by biophysical measurements of several non-enzymic mesophilic proteins at about -65 degrees C and reflecting a sharp decrease in protein flexibility as the overall motion of groups of atoms ceases. ...
Plant endo-β-mannanases (EC 3.2.1.78) catalyze the hydrolysis of mannan polysaccharides randomly through a retaining mechanism. In Arabidopsis thaliana, there are 8 mannanases identified and AtMAN7 is one of those. In the present investigation, the AtMAN7 gene was cloned and the recombinant protein was expressed in E. coli. Most of the protein yielded in inclusion bodies, but pure protein could be obtained from the protein supernatant using IMAC. The dinitrosalicylic acid (DNS) method was used to measure enzyme activity. The result showed that the optimal temperature of AtMAN7 was 40-45 °C and that the enzyme had a pH optimum at pH 5. T-DNA insertional mutant Arabidopsis seeds were used to investigate the mannanase function in situ. The germination rate of gene AtMAN6 and AtMAN7 knockouts was much higher than the wild type, while AtMAN1 knockouts germinated at a slower rate compared to wild type. Thus, this indicated that AtMAN6 and AtMAN7 had a negative effect and AtMAN1 had a positive effect ...
Purchase high purity enzyme endo-1-4 beta-Mannanase (Bacillus sp.) for for use in research, biochemical enzyme assays and in vitro diagnostic analy...
a Crude β-mannanase recovery from solid medium. b Crude β-mannanase recovery from liquid medium. Data shown here are average of quadruplicate measurements wit
SWISS-MODEL Repository entry for A1C8U0 (MANF_ASPCL), Mannan endo-1,4-beta-mannosidase F. Aspergillus clavatus (strain ATCC 1007 / CBS 51365 / DSM 816 / NCTC 3887 /NRRL 1)
SWISS-MODEL Template Library (SMTL) entry for 6t75. Bacteroides salyersiae GH164 beta-mannosidase 2-deoxy-2-fluoro-beta-D-mannosyl enzyme intermediate
K01186 NEU1; sialidase-1 [EC:3.2.1.18] K01190 lacZ; beta-galactosidase [EC:3.2.1.23] K12373 HEXA_B; hexosaminidase [EC:3.2.1.52] K12373 HEXA_B; hexosaminidase [EC:3.2.1.52] K12373 HEXA_B; hexosaminidase [EC:3.2.1.52] K01192 E3.2.1.25; beta-mannosidase [EC:3.2.1.25] K01201 GBA; glucosylceramidase [EC:3.2.1.45 ...
Novo Servidor Tibinha-Go Global Data da Inauguração será dia 14/04/2019 ás 13:00 O site já está liberado para criar sua Conta Cliente Próprio 11x (Paladin não usa Escudo, as Armas são de 2 Mãos e Munições infinitas) Itens Vip Segunda Promotion  Mapa Global mais informações acesse nossa Página no facebook: https://www.facebook.com/Tibinha-Go-321118205258255/ Site do servidor tibinha-go.ddns.net Chame seus amigos e venha se aventurar no Tibinha-Go (EM BREVE MUITAS NOVIDADES) Regeneração Knight Elite Knight Pirokudo Knight HP - 10 HP - 25 Hp - 50 Mana - 5 Mana - 10 Mana - 20 Paladin Royal Paladin Pirokudo Paladin Hp - 7 HP - 18 HP - 35 Mana - 7 Mana - 18 Mana - 35 Sorcerer Master Sorcerer Pirokudo Sorcerer Hp - 5 HP - 10 HP - 20 Mana - 10 Mana - 25 Mana - 50 Druid Elder Druid Pirokudo Druid Hp - 5 HP - 10 HP - 20 Mana - 10 Mana - 25 Mana - 50 PvP Protection: to level 50 Skill Rate: 40x Magic Rate: 20x Loot Rate: 2x Kills to RedSkull: 8x Protection Level: 50 Exp Rate per stage: ...
Mannanases - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
Rabbit polyclonal antibody raised against synthetic peptide of MNDA. A synthetic peptide corresponding to MNDA. (PAB25135) - Products - Abnova
β-mannanase (EC 3.2.1.78); exo-β-1,4-mannobiohydrolase (EC 3.2.1.100); β-1,3-xylanase (EC 3.2.1.32); lichenase / endo-β-1,3-1,4-glucanase (EC 3.2.1.73); mannobiose-producing exo-β-mannanase (EC 3.2.1.- ...
Comprar Laxar Purgante Natural El Mana Online. Venta del laxante natural Laxar de El Mana, mejor purgante ✅ Tienda Naturista Bogotá ✅ Envío Rápido
Apabila kita dah lalui sendiri taklah ngeri mana, sakit tu memang sakit tapi sakit itu hilang apabila kita dapat tengok zuriat sendiri depan mata lagi-lagi ini anak sulung, orang kata confirm kena gunting, lepas tu kau kena jahit, nak buang air besar pun susah nanti tapi alhamdulillah atas izin Allah, nak melahirkan Adam Malique ni tiada luka terkoyak dan kena jahit. Baby keluar 2.8 kilogram dan ramai juga tanya macam mana boleh tiada koyak, hebat. Saya tak hebat cuma rezeki saya bersalin tiada kena jahit, katanya menerusi akaun Facebook miliknya, malam tadi. ...
Ive been working on new design which has the following goals: Align all spellcasting classes to use short rest recovery mechanics. This will encourage short rests and significantly alter the external balance between pure martials and casters so that casters can t simply cast all of their spells in 1 encounter. Use mana (spell points) instead of spell slots for all spellcasting classes. Provide less duplication and more clarity to a user s character choice by providing a base class and
This weeks Caffiend starts with a couple of caveats: 1) We bought this Mana Energy Potion at Hot Topic, and 2) We were warned to drink only half of the 1.6 ounce bottle. We raise the first point because if an energy supplement is available at Hot Topic, its supposed...
Spor kolem reprezenta n smlouvy dvojn sobn olympijsk v t zky Ester Ledeck nevznikl kv li v i st tn podpory, kterou prost ednictv m svazu dost v , ale kv li omezuj c m podm nk m svazu pro v b r komer n ch sponzor . Tvrd to agentura Sport Invest, kter za Ledeckou smlouvu vyjedn v . Nov n vrh, kter po tern m jedn n v konn ho v boru podepsali funkcion i svazu, je t mana e i Ledeck nevid li. Nen proto jasn , zda byla problematick pas n jak upravena.
@horvath heres my vintage pox list in its last iteration. I played it a few times. I did pretty well with it once. If you catch the right xerox decks you can just go under them and destroy their mana base. Its a lot of fun regardless. Very light on win...
Cumpara Idei creative 87 - Papusi de mana - Kocsis Eszter pe Libris. Transport gratuit >90 lei si livrare rapida. 30 de zile retur.
A few cut corners never hurt anyone -- or, at least, that seems to be the thinking behind Adventures of Mana foray onto the Vita.
Secret of Mana Project This is a continiuation of the SoM project thread by Bahamut. Since Bahamut has much less time on his hands to co-direct ...
My max mana: 11115 I cast flash heal: 2300 I get 87 mana back. Edit: If Im suppose to get back 2.5% on a flash heal, that means I should be getting back 277. Which I clearly am not. Does anyone know if this is a bug... or is what Rapture means: You can only get back a total of 2.5% mana on a big heal... So far from Im reading into - this new Rapture totally sucks (like I said when it first came out) Lets hope Im wrong.
This item is expected to be in stock on the . Orders containing this item will not be shipped before the official release date. Special preorder price until release ...
Hōʻike nā hōʻike Covid i koi ʻia e ka ʻāina i ka nele i ka like ʻole. Ua hoʻokumu kēlā me kēia o lākou i kā lākou mau hae a me nā koi.
https://www.mtggoldfish.com/articles/vintage-101-aether-revolt-part-two Islandswamp reviews some Aether Revolt cards for Vintage and tells you how to find homes for newly banned Standard and Modern cards. Also check out this link here: https://www.mtggold...
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Mana Whakahono ā Rohe is a new tool designed to assist tangata whenua and local authorities discuss, agree and record how they will work together under the Resource Management Act. This guidance explains how the legislation can be implemented and provides ideas about what could be included in a Mana Whakahono ā Rohe arrangement. Read more ...
Mana Whakahono ā Rohe is a new tool designed to assist tangata whenua and local authorities discuss, agree and record how they will work together under the Resource Management Act. This guidance explains how the legislation can be implemented and provides ideas about what could be included in a Mana Whakahono ā Rohe arrangement. Read more ...
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Perhaps I just forgot how leveling is, and no this isnt about leveling in Wrath being too hard or too slow. I just find my spriest (currently lv73) to have to refrain from using some spells too often otherwise hes constantly struggling with low mana. Thats inspite of Shadowfiend and Dispersion. Thus Im feeling slowed down by that. Whats your take? Is mana an issue for you?
Is it possible to exclude specific drives from disk full alerts? We have a couple of servers where the application mana... | 21 replies | Spiceworks Support
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
Osmolalitas urin adalah ukuran konsentrasi urin , di mana nilai besar menunjukkan urin pekat dan nilai kecil menunjukkan urin encer. Konsumsi air (termasuk air yang terkandung dalam makanan) mempengaruhi osmolalitas urin. ...
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
Macam mana nak buat kuah lebih pekat? Selepas Yui masak, yui hanya ambil sedikit sahaja iaitu cukup untuk satu hidangan. Ketika menggelegak, terus masukkan bancuhan tepung jagung ( sikit saja ) dan terus kacau. Autotamatik kuah akan jadi pekat dan likat seperti gambar bawah ...
alhamdulillah,,,habis bulan march,,beerti sudah sebulan jadi fullhousewife, alhamdulillah urusan kerja dpt d bereskan dgn sempurna, rutin harian juga automatik berubah, yg best dapat menghabiskan masa yg byk dgn family, dapat biat pelbagai urusan, business dan mcm2, 1st time bila terlintas d fikiran amik keputusan nk resign mcm2 dlm kepala memikirkan,,,,,dari pelbagai sudut terutama ekonomi, ye la hanya bergantung pd ekonomi suami shj,,,,namun benar janjiNya,,,,rezeki ada d mana2, janji kita berusaha, subhanallah,,,,,,,,,doa setiap hari semoga d permudahkan segalanya,,,,amin 3x, yg paling ketara, anak2 makin sihat, semua berisi....syukran dan yg best dpt ajar sendiri mengaji al quran, solat ...
Ngāti Whātua Ōrākei innovation officer Te Aroha Grace says AI can help to combine the values from different cultures to help grow Aotearoas mana and brand - and AI is not just for commercial gain.
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Bromine atom in PDB 2vqt: Structural and Biochemical Evidence For A Boat-Like Transition State in Beta-Mannosidases
The microbial degradation of the plant cell wall is a pivotal biological process that is of increasing industrial significance. One of the major plant structural polysaccharides is mannan, a beta-1,4-linked d-mannose polymer, which is hydrolyzed by endo- and exo-acting mannanases. The mechanisms by which the exo-acting enzymes target the chain ends of mannan and how galactose decorations influence activity are poorly understood. Here we report the crystal structure and biochemical properties of CjMan26C, a Cellvibrio japonicus GH26 mannanase. The exo-acting enzyme releases the disaccharide mannobiose from the nonreducing end of mannan and mannooligosaccharides, harnessing four mannose-binding subsites extending from -2 to +2. The structure of CjMan26C is very similar to that of the endo-acting C. japonicus mannanase CjMan26A. The exo-activity displayed by CjMan26C, however, reflects a subtle change in surface topography in which a four-residue extension of surface loop creates a steric block at ...
Accepted name: α-mannan endo-1,2-α-mannanase. Reaction: Hydrolysis of the terminal α-D-mannosyl-(1→3)-α-D-mannose disaccharide from α-D-mannosyl-(1→3)-α-D-mannosyl-(1→2)-α-D-mannosyl-(1→2)-α-D-mannosyl side chains in fungal cell wall α-mannans.. Systematic name: α-mannan glucosylmannohydrolase. Comments: The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-α-1,2-mannosidase.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: References:. 1. Hakki, Z., Thompson, A.J., Bellmaine, S., Speciale, G., Davies, G.J. and Williams, S.J. Structural and kinetic dissection of the endo-α-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp. Chemistry 21 (2015) 1966-1977. [PMID: 25487964]. 2. Cuskin, F., Lowe, E.C., Temple, M.J., Zhu, Y., Cameron, E.A., Pudlo, N.A., Porter, N.T., Urs, K., Thompson, A.J., Cartmell, ...
Buy high purity recombinant enzyme endo-1-4-beta-Mannanase Cellvibrio japonicus for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
3.2.1.1 Alpha-amylase 3.2.1.2 Beta-amylase 3.2.1.3 Glucan 1,4-alpha-glucosidase 3.2.1.4 Cellulase 3.2.1.5 Deleted entry 3.2.1.6 Endo-1,3(4)-beta-glucanase 3.2.1.7 Inulinase 3.2.1.8 Endo-1,4-beta-xylanase 3.2.1.9 Deleted entry 3.2.1.10 Oligo-1,6-glucosidase 3.2.1.11 Dextranase 3.2.1.12 Transferred entry: 3.2.1.54 3.2.1.13 Transferred entry: 3.2.1.54 3.2.1.14 Chitinase 3.2.1.15 Polygalacturonase 3.2.1.16 Deleted entry 3.2.1.17 Lysozyme 3.2.1.18 Exo-alpha-sialidase 3.2.1.19 Deleted entry 3.2.1.20 Alpha-glucosidase 3.2.1.21 Beta-glucosidase 3.2.1.22 Alpha-galactosidase 3.2.1.23 Beta-galactosidase 3.2.1.24 Alpha-mannosidase 3.2.1.25 Beta-mannosidase 3.2.1.26 Beta-fructofuranosidase 3.2.1.27 Deleted entry 3.2.1.28 Alpha,alpha-trehalase 3.2.1.29 Transferred entry: 3.2.1.52 3.2.1.30 Transferred entry: 3.2.1.52 3.2.1.31 Beta-glucuronidase 3.2.1.32 Endo-1,3-beta-xylanase 3.2.1.33 Amylo-alpha-1,6-glucosidase 3.2.1.34 Transferred entry: 3.2.1.35 3.2.1.35 Hyaluronoglucosaminidase 3.2.1.36 ...
여러 종류의 mannanase를 생산하는 Cellulosimicrobium sp. YB-43으로부터 mannanase B를 암호하는 manB 유전자와 효소의 특성이 보고된 바 있다. Mannanase C (ManC)로 명명한 효소의 유전자가 manB 유전자의 하류에 위치한 것으로 예상되어 이를 중합효소 연쇄반응으로 클로닝하여 manC 유전자의 염기서열을 결정하였다. ManC는 448 아미노산 잔기로 구성된 것으로 확인되었으며 glycosyl hydrolase family 5에 속하는 mannanase와 상동성이 높은 활성영역과 탄수화물 결합영역(CBM2)이 존재하였다. ManC의 활성영역은 Streptomyces sp. SirexAA-E (55.8%; 4FK9_A) 및 S. thermoluteus (57.6%; BAM62868)의 mannanase와 아미노산 배열의 상동성이 55% 이상으로 가장 높았다. Signal peptide 영역이 제거되고 카르복실 말단에 hexahistidine이 연결되도록 제조한 His-tagged ManC (HtManC)의 유전자를 재조합 대장균에서 발현하여 균체 ...
Bacteroides ovatus ATCC ® 8483™ Designation: TypeStrain=True Application: Quality control strain for API and BBL products. Control strain for anaerobe identification.
Bacteroides ovatus ATCC ® 8483™ Designation: TypeStrain=True Application: Quality control strain for API and BBL products. Control strain for anaerobe identification.
Bacteroides ovatus is a member of the human gut microbiota. The importance of this microbial consortium involves the degradation of complex dietary glycans mainly conferred by glycoside hydrolases. In this study we focus on one such catabolic glycoside hydrolase from B. ovatus. The enzyme, termed BoMan26A, is a -mannanase that takes part in the hydrolytic degradation of galactomannans. The crystal structure of BoMan26A has previously been determined to reveal a TIM-barrel like fold, but the relation between the protein structure and the mode of substrate processing has not yet been studied. Here we report residue-specific assignments for 95% of the 344 backbone amides of BoMan26A. The assignments form the basis for future studies of the relationship between substrate interactions and protein dynamics. In particular, the potential role of loops adjacent to glycan binding sites is of interest for such studies. ...
Lipid/Header}} {{Hierarchy,{{PAGENAME}}}} {{#googleapi:}} {{Glycolipid ,Structure=Xylb1-4(Rhaa1-3)Mana1-2Mana1-6(Glca1-2Mana1-3)Mana1-4(AEPn-6)GlcNa1-6Ins-P-Cer ,Mainchain=Xylb1-4Mana1-2Mana1-6Mana1-4GlcNa1-6Ins-P-Cer ,Species=Herpetomonas (Protista); ,Phylum=Protista ,Source=Herpetomonas pessoai (Protozoan) ,Lipidbank=- ,Common Name=- ,Abbrev=- ,Cfg=11 3 6; K 0 0; T 0 3; O 1 0; J 1 1; J 1 2; J 1 3; E 1 4; L 1 5; Z 1 6; A 2 1; J 2 2; 1 4; 2 7; 3 4; 4 5; 5 6; 6 7; 7 8; 8 9; 10 11; 11 6; ,Root=Cer }} {{Lipid/Footer ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Among other things, the fats in Mana help regulate cholesterol and supply quick energy to the body and brain. Each portion of our drink contains a 16-g mixture of oils from canola, algae (rich in DHA and EPA omega-3s), coconut, sunflower, flaxseed, oats, and phospholipids from soy lecithin.. Mana delivers a beneficial 2:1 ratio of omega-6 to omega-3 fatty acids ...
Thats all there is to the Unlimited Mana option, but this basic framework can be built on to make all sorts of dramatically different uses of the GURPS magic rules. Consider any of the following: Emergencies Only GURPS Magic rules are standard, and mages may still expend ST to power their spells. However, they may draw additional power out of the ether via Unlimited Mana, but the campaign Threshold is zero, and may not be improved. This means that any free lunch casting automatically causes a Calamity Check. RR should probably stay in the low range (1-10 daily). A slight (10-point) Unusual Background for mages would be appropriate; this option makes magic more powerful than in the normal rules. Spending Options Mages may spend energy to make their spells faster (4 points per second of reduction, which will even affect missile spells and bring spells to zero time if sufficient energy is spent). They may also increase their odds (1 point of energy per +1, or 3 per +1 if the spell is to be ...
aku nak sangat benda ni..tapi tak tau nak carik kat mana...korang bule tolong bagi tau tak kat mana ade..and berapa ek harganya..??aku pernah jumpe kat giant.
Free delivery and returns on eligible orders of £20 or more. Buy adidas Mens Mana Bounce 2 M Aramis Running Shoes at Amazon Fashion.
Dr Manas Gorey is a practising Opthalmologist in Chennai. Book appointments with the best medical professionals for Opthalmology at Ask Apollo.
Německo vynalezlo socialismus. Karel Marx a Bedřich Engels byli Němci. Sociálně demokratické hnutí, které zformovalo moderní evropský sociální stát, také pochází z Německa. Přestože pro zemi její opětovné začlenění do světové obchodní soustavy po druhé světové válce znamenalo obrovský přínos, Německo se nikdy skutečně nevyrovnalo s anglosaským kapitalismem, který stále obklopuje hluboká skepse. Dnes, když už německé hospodářství nefunguje hladce, kapitál se přesouvá za hranice a nezaměstnanost roste, získává kritika kapitalismu opět na síle. Veřejnost je rozezlena platy vrcholných manažerů a skutečností, že velké německé společnosti propouštějí navzdory zaznamenaným ziskům. Vláda zareagovala naléháním na manažery, aby zveřejnili své příjmy, a zrušením zákonů vymezujících bankovní tajemství, jež kdysi bývaly nedotknutelné. Tato nová kritika kapitalismu nedávno vyvrcholila sérií útoků lídra ...
Maksud GLC ? GLC ditakrif sebagai syarikat yang mempunyai objektif komersil yang primer dan kepentingannya dikawal secara langsung oleh Kerajaan Malaysia. Mengawal kepentingan merujuk kepada keupayaan atau kuasa Kerajaan (bukan sekadar peratus pemilikan) untuk melantik anggota Lembaga Pengarah, pengurusan atasan dan membuat keputusan utama terhadap GLC (contohnya, pemberian kontrak, strategi, penstrukturan semula dan pembiayaan, pemerolehan serta pemberhentian pelaburan), sama ada secara langsung atau melalui Syarikat Pelaburan Berkaitan Kerajaan (GLIC ...
Sebenarnya ini hasil pengamatan sudah lama, cuma belum sempat memilah mana yang harus saya simpan dan mana yang mau saya share dengan kawan - kawan semua. Caladi Ulam atau Dendrocopus macei ini biasa kita kenal dengan sebutan Burung Pelatuk. Ya, dia memang masuk kelompok burung pelatuk. Kebiasaannya melubangi kayu - kayu yang lapuk. Ini saya…
Eg. Exiva Daron Digur = Exiva Daron~. Its useful to locate friends during hunts or enemies during wars. Note that when you are trying to exiva a GM or CM, you cannot use the ~ symbol. Since the 2007 summer update, using the spell on a character who is offline, do not exists or is ambiguous, will still consume 20 mana. In other words, casting this spell will always cost mana, working or not. Results explanation * ...
Blog despre fotografie, tendinte si tot ce tine de sculptarea in lumina moderna. Despre fotografii romani contemporani si imaginile capturate de obiectivele lor. Un blog care da idei de proiecte fotografice, de unde aflii ce e nou dar si ce e celebru in fotografia internationala si un blogroll din ce in ce mai lung...
Setting up a computational cluster (HPC), part 2 - Now that we can easily provide DHCP, DNS and TFTP and a debian image for all the nodes, we want to make it easy to maintain the cluster and setup user mana... ...
Kardiotrofin-1 (CT-1) je citokin. On je srčani hipertrofni faktor sa 21.5 kDa, i član IL-6 citokinske familije. CT-1 je povezan sa patofiziologijom srčanih bolesti[1], kao što su hipertenzija, miokardijalna infarkcija, valvularna srčana mana, and kongestivna srčana slabost. Ovaj protein deluje putem interakcije sa glikoproteinom 130 (gp130)/leukemijskim inhibitornim faktorom receptor beta (LIFR) heterodimera. Dodatno, CT-1 aktivira fosfatidilinozitol 3-kinazu (PI-3 kinaza) u srčanim mišićima i pojačava DNK-vezivanje transkripcionog faktora NF-κB. CT-1 je visoko izražen u srcu, skeletalnim mišićima[2], prostati i jajnicima, i u manjoj meri u plućima, bubrezima, pankreasu, timusu, testisima i tankim crevima.[3][4][5]. ...
Profesné zameranie - hodnotenie kvality vôd v povrchových tokoch a nádržiach, ochrana a prognózy kvality vody, transport a transformácia látok vo vodnom prostredí povrchových tokov, manažment kvality vody v povodí tokov, hodnotenie kvality dnových sedimentov v tokoch a nádržiach, štúdium interakcií medzi vodou a sedimentmi. Publikace v domácích a zahraničních časopisech a sbornících. HUCKO, P.: Úprava prirodzene zafarbenývh vôd priamou filtráciou. In: Sborník ...
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SEJAUH mana anda menyedari kepentingan ujian saringan dan menjalani pemeriksaan itu? Kebanyakan orang kurang sedar malah ada segelintir tidak tahu langsung apak
malam al-qadar adalah satu malam dlm bln Ramadhan yg ditentukan di mana Allah shj yg mengetahuimya. Malam itu yg mempunyai pelbagai kelebihan dan keistimewaan yg melebihi 1000 bulan. Ini ditegaskan Allah di dalam surah al-Qadr, ayat 1 hingga 3 , yg bermaksud ...
18 Des 2017 - Sewa dari orang-orang di Honomu, Hawaii mulai dari Rp271950/malam. Temukan tempat menginap unik dengan tuan rumah lokal di 191 negara. Serasa di rumah di mana saja bersama Airbnb.
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Avantajele sunt minuscule fata de cat timp se pierde, ani de zile. Multe chestii se stiau si s-au pierdut in timp, probabil scrisul de mana o sa-l mai
Pengambilan minyak zaitun menambahkan bilangan kolesterol baik untuk badan di mana ia menghalang penghasilan plak pada dinding arteri dan kehadiran antiaksida dalam minyak zaitun juga mengurangkan risiko tekanan darah tinggi ...
PENDEKATAN PASIEN IKTERUS ANAMNESIS RPS : 1. Kapan pertama kali memperhatikan adanya mata kuning ? Apakah makin hari makin bertambah warna kuningnya ? 2. Adakah gejala lain seperti : - Nyeri abdomen di daerah mana ? sejak kapan ? dan bagaimana karakteristiknya ? ; yaitu untuk mencari kemungkinan kolesistitis atau batu empedu yang bisa menimbuylkan…
Each player may play an additional land during each of his or her turns. Whenever a land is tapped for mana, return it to its owners hand.
WIRO SABLENG Pendekar Kapak Maut Naga Geni 212 Karya: Bastian Tito PEMUDA baju biru itu berdiri dengan gagahnya di puncak bukit. Angin dari timur bertiup melambai-lambaikan rambutnya yang gondrong menjela bahu. Sepasang matanya sejak tadi hampir tiada berkesip memandang lekat-lekat ke arah utara di mana berdiri dengan megahnya pintu gerbang Kotaraja. Sudah hampir setengah hari…
feeling much better. semalam akhirnya gue memutuskan untuk makan obat lodia dan minum obat maag plantacid buat ngilangin nyeri. lumayan hari ini enakan perut. nyeri berkurang dan ke belakang gak sesering tiga hari lalu yang mana kayak dikuras habis semua isi perut. perjuangan banget dah ...
6-phospho-beta-glucosidase (EC 3.2.1.86); lactase-phlorizin hydrolase (EC 3.2.1.62), lactase (EC 3.2.1.108); beta-mannosidase ( ... beta-galactosidase (EC 3.2.1.23); 6-phospho-beta-galactosidase (EC 3.2.1.85); ... Glycoside hydrolase family 1 CAZY GH_1 comprises enzymes with a number of known activities; beta-glucosidase (EC 3.2.1.21); ... EC 3.2.1.25); myrosinase (EC 3.2.1.147). 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT; LCTL; GH1 in ...
... beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a conserved glutamic acid residue which ... The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... Gebler JC, Aebersold R, Withers SG (June 1992). "Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta- ... These enzymes also include an immunoglobulin-like beta-sandwich domain. GH2 in CAZypedia Henrissat B, Callebaut I, Fabrega S, ...
4-beta-mannosidases. Mannan endo-1,4-beta-mannosidase hydrolyses mannan and galactomannan, but displays little activity towards ... Braithwaite KL, Black GW, Hazlewood GP, Ali BR, Gilbert HJ (February 1995). "A non-modular endo-beta-1,4-mannanase from ... The enzyme randomly hydrolyses 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans and galactoglucomannans. Henrissat ...
... may refer to: Beta-mannosidase, an enzyme A branch of the alternative fashion trend Ganguro This disambiguation page ...
N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase and esterase-lipase (C8) are negative. Acid is produced from ... beta-galactosidase, beta-glucuronidase, pyrrolidonyl arylamidase, esterase (C4), lipase (C14), Naphthol-AS-BI-phosphohydrolase ...
... reductase deficiency Lysinuric protein intolerance Lysosomal alpha-D-mannosidase deficiency Lysosomal beta-mannosidase ...
... is an enzyme which hydrolyses mannose. There are two types: alpha-Mannosidase beta-Mannosidase A deficiency is ... Mannosidases at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal v t e. ... A family of mannosidases are also responsible for processing newly formed glycoproteins in the endoplasmic reticulum into ...
... , also called lysosomal beta-mannosidase deficiency, is a disorder of oligosaccharide metabolism caused by ... Beta-mannosidase Alpha-mannosidosis "Mannosidosis, beta A, lysosomal , Genetic and Rare Diseases Information Center (GARD) - an ... "OMIM Entry - * 609489 - MANNOSIDASE, BETA A, LYSOSOMAL; MANBA". www.omim.org. Retrieved 9 May 2018. Johnson, William (2015). ... decreased activity of the enzyme beta-mannosidase. This enzyme is coded for by the gene MANBA, located at 4q22-25. Beta- ...
A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... A new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) which is ... Beta-gal is inhibited by L-ribose, non-competitive inhibitor iodine, and competitive inhibitors 2-phenylethyl 1-thio-beta-D- ... April 2006). "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111 ...
... (EC 3.2.1.25, mannanase, mannase, beta-D-mannosidase, beta-mannoside mannohydrolase, exo-beta-D-mannanase, ... 1998). "Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta- ... lysosomal beta A mannosidase) is an enzyme with systematic name beta-D-mannoside mannohydrolase, which is in humans encoded by ... 1994). "Human beta-mannosidase deficiency associated with peripheral neuropathy". Ann. Neurol. 35 (1): 116-9. doi:10.1002/ana. ...
... (EC 3.2.1.152, endo-beta-mannosidase) is an enzyme. This enzyme catalyses the ... Mannosylglycoprotein+endo-beta-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology ... Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S (September 2004). "Endo-beta-mannosidase, a plant enzyme acting ... "Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium ...
... (EC 3.2.1.78, endo-1,4-beta-mannanase, endo-beta-1,4-mannase, beta-mannanase B, beta-1, 4- ... beta-D-mannanase, 1,4-beta-D-mannan mannanohydrolase) is an enzyme with systematic name 4-beta-D-mannan mannanohydrolase. This ... Mannan+endo-1,4-beta-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... enzyme catalyses the following chemical reaction Hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and ...
6-beta-mannanase, endo-alpha-1->6-D-mannanase, endo-1,6-beta-mannanase, mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan ... Mannan+endo-1,6-alpha-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Mannan endo-1,6-alpha-mannosidase (EC 3.2.1.101, exo-1, ...
... alpha/beta-galactosidase, β-glucuronidase, α-fucosidase, α-mannosidase, and trypsin. Important dual virulence factors found in ...
... mannosidases MeSH D08.811.277.450.625.500 - alpha-mannosidase MeSH D08.811.277.450.625.750 - beta-mannosidase MeSH D08.811. ... 4-beta-glucosidase MeSH D08.811.277.450.420.200.600 - glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 - glucan ... 4-beta-cellobiosidase MeSH D08.811.277.450.420.200.450 - endo-1,3(4)-beta-glucanase MeSH D08.811.277.450.420.200.500 - glucan 1 ... 4-beta xylanases MeSH D08.811.277.450.950.500 - xylan endo-1,3-beta-xylosidase MeSH D08.811.277.656.149 - atp-dependent ...
... is a deficiency in mannosidase, an enzyme. There are two types: Alpha-mannosidosis Beta-mannosidosis Swainsonine ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
... beta-D-glucosidase, beta-glucoside glucohydrolase, arbutinase, amygdalinase, p-nitrophenyl beta-glucosidase, primeverosidase, ... Beta-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and ... Vicianin beta-glucosidase. References[edit]. *^ PDB: 3AHX​; Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang ... amygdalase, linamarase, salicilinase, and beta-1,6-glucosidase. Cellulose is a polymer composed of beta-1,4-linked glucosyl ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ... In S-glycosylation, a beta-GlcNAc is attached to the sulfur atom of a cysteine residue.[4] ...
... alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I". The Journal of Biological Chemistry. 255 (10): 4894-902. PMID ... mannosidase II, exo-1,3-1,6-alpha-mannosidase, alpha-D-mannosidase II, alpha-mannosidase II, alpha1-3,6-mannosidase, GlcNAc ... Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes ... transferase I-dependent alpha1,3[alpha1,6]mannosidase, Golgi alpha-mannosidase II, ManII, 1,3(1,6)-alpha-D-mannosidase, 1,3-(1, ...
... mannosidase deficiency diseases MeSH C18.452.648.202.607.500 - alpha-mannosidosis MeSH C18.452.648.202.607.750 - beta- ... mannosidase deficiency diseases MeSH C18.452.648.595.577.500 - alpha-mannosidosis MeSH C18.452.648.595.577.750 - beta- ...
... mannosidase deficiency diseases MeSH C16.320.565.202.607.500 - alpha-mannosidosis MeSH C16.320.565.202.607.750 - beta- ... mannosidase deficiency diseases MeSH C16.320.565.580.577.500 - alpha-mannosidosis MeSH C16.320.565.580.577.750 - beta- ... beta-thalassemia MeSH C16.320.077.090 - anemia, Diamond-Blackfan MeSH C16.320.077.280 - fanconi anemia MeSH C16.320.099.037 - ... beta-thalassemia MeSH C16.320.400.024 - Alexander disease MeSH C16.320.400.050 - amyloid neuropathies, familial MeSH C16.320. ...
6-alpha-D-mannosidase EC 3.2.1.164: galactan endo-1,6-beta-galactosidase EC 3.2.1.165: exo-1,4-beta-D-glucosaminidase EC 3.2. ... beta-D-glucopyranosyl abscisate beta-glucosidase EC 3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end) EC 3.2.1.177: ... glycyrrhizinate beta-glucuronidase EC 3.2.1.129: endo-a-sialidase EC 3.2.1.130: glycoprotein endo-a-1,2-mannosidase EC 3.2. ... a-mannosidase EC 3.2.1.78: mannan endo-1,4-b-mannosidase EC 3.2.1.79: deleted, included in EC 3.2.1.55 EC 3.2.1.80: fructan b- ...
Processing of asparagine-linked oligosaccharides by one or more rat liver Golgi alpha-D-mannosidases dependent on the prior ... action of UDP-N-acetylglucosamine: alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I". J Biol Chem. 255 (10): 4894-902 ...
DEFB118, DEFB119, DEFB126, DEFB127, DEFB129: Beta-defensin genes. *DLGAP4: Disks large-associated protein 4 ... EDEM2: ER degradation-enhancing alpha-mannosidase-like 2. *EDN3: endothelin 3. *ENTPD6: Ectonucleoside triphosphate ...
... beta) MEF2C: Myocyte-specific enhancer factor 2C MEF2C-AS1: encoding protein MEF2C antisense RNA 1 MGAT1: Mannosyl (alpha-1,3 ... Alpha-mannosidase 2 MASS1: monogenic, audiogenic seizure susceptibility 1 homolog (mouse) MCC: Colorectal mutant cancer protein ... encoding protein Amyloid beta A4 precursor protein-binding family B member 3 APC: adenomatosis polyposis coli ARL15: encoding ... beta polypeptide) HMGXB3: encoding protein HMG-box containing 3 IK: Protein Red IRX1: Iroquois-class homeodomain protein (human ...
This enzyme catalyses the following chemical reaction Endohydrolysis of (2->1)-beta-D-fructosidic linkages in inulin Adams M, ... glucosidases and mannosidases". J. Am. Chem. Soc. 65: 1369-1380. doi:10.1021/ja01247a029. Inulinase at the US National Library ... Inulinase (EC 3.2.1.7, inulase, endoinulinase, endo-inulinase, exoinulinase, 2,1-beta-D-fructan fructanohydrolase) is an enzyme ... with systematic name 1-beta-D-fructan fructanohydrolase. ...
... beta polypeptide (maple syrup urine disease) (6q14.1) BMIQ3: body mass index QTL 3 TMEM242 encoding transmembrane protein ... mannosidase alpha class 1A member 1 (6q22.31) MB21D1: encoding protein Mab-21 domain containing 1 MCDR1: macular dystrophy, ... N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (6p24.3) GMDS: GDP-mannose 4,6-dehydratase (6p25.3) HCG4P11: ... cAMP-dependent protein kinase inhibitor beta (6p22.31) PLAGL1: (6q24.2) RCD1: retinal cone dystrophy 1 RP63: retinitis ...
B-mannosidase deficiency Carotenosis Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy ... broad beta disease, remnant removal disease) Familial hypertriglyceridemia Farber disease (fibrocytic dysmucopolysaccharidosis ...
Gu, H; Marth, JD; Orban, PC; Mossmann, H; Rajewsky, K (1 July 1994). "Deletion of a DNA polymerase beta gene segment in T cells ... "Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis ... Ohtsubo, K.; Chen, M. Z.; Olefsky, J.M.; Marth, J.D. (2011). "Pathway to diabetes through attenuation of pancreatic beta cell ... Their research showed that the malfunction of pancreatic beta cells was the major contributor to disease onset. Their research ...
Evidence for an alpha', beta Mechanism". J. Am. Chem. Soc. 94 8608-. Reich, H. J., Peake, S. L. (1978). "Hypervalent ... Inhibitors of B-Mannosidases?". Helv. Chim. Acta. 85 1118-. Cope, A.C.; Mehta, A.S. (1963). "Mechanism of the Hofmann ...
... endo-N-acetyl-beta-D-glucosaminidase, endo-N-acetyl-beta-glucosaminidase, endo-beta-N-acetylglucosaminidase D, endo-beta-N- ... H is able to cleave each structure of this oligosaccharide as it is processed until the enzyme Golgi alpha-mannosidase II ... endo-beta-acetylglucosaminidase, endo-beta-(1->4)-N-acetylglucosaminidase, mannosyl-glycoprotein 1,4-N-acetamidodeoxy-beta-D- ... endo-beta-N-acetylglucosaminidase L, glycopeptide-D-mannosyl-4-N-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-beta- ...
Each ring closure can have either an alpha or beta configuration at the anomeric position. The chemical rapidly undergoes ... Glucose is hydrolyzed on fully folded protein and the mannose moieties are hydrolyzed by ER and Golgi-resident mannosidases. ... α-Mannosidase Mannose receptor Mannan oligosaccharide-based nutritional supplements Rhamnose, 6-deoxy-L-mannose Freeze, H. H.; ...
Youm, J.; Jeon, J.; Kim, H.; Kim, Y.; Ko, K.; Joung, H.; Kim, H. (2008). "Transgenic tomatoes expressing human beta-amyloid for ... α-mannosidase and β-D-N-acetylhexosaminidase. The fruits produced were not visibly damaged after being stored at room ...
Jacob CA, Bodmer D, Spirig U, Battig P, Marcil A, Dignard D, Bergeron JJ, Thomas DY, Aebi M (2001). "Htm1p, a mannosidase-like ... Lenter M, Vestweber D (Apr 1994). "The integrin chains beta 1 and alpha 6 associate with the chaperone calnexin prior to ... Hosokawa N, Wada I, Hasegawa K, Yorihuzi T, Tremblay LO, Herscovics A, Nagata K (2001). "A novel ER alpha-mannosidase-like ... Yos-9 recognizes mannose residues exposed after α-mannosidase removal of an outer mannose of misfolded glycoproteins. Calnexin ...
... beta; acid (includes glucosylceramidase) (gene for Gaucher disease) GBAP1: glucosylceramidase beta pseudogene 1 GLC1A: gene for ... 2-alpha-mannosidase IB MEAF6: MYST/ESA1 associated factor 6 MECR: Trans-2-enoyl-CoA reductase, mitochondrial MFAP2: ... beta 3, 31kDa (connexin 31) GLMN (1p22) GNL2: G protein nucleolar 2 GSTM1 (1p13) HDAC1 (1p35) HES2: Hes family bHLH ... beta TTC39A: Tetratricopeptide repeat 39A UBR4: E3 ubiquitin-protein ligase component n-recognin 4 UROD: uroporphyrinogen ...
Beta-mannosidase (EC 3.2.1.25, mannanase, mannase, beta-D-mannosidase, beta-mannoside mannohydrolase, exo-beta-D-mannanase, ... 1998). "Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta- ... lysosomal beta A mannosidase) is an enzyme with systematic name beta-D-mannoside mannohydrolase, which is in humans encoded by ... 1994). "Human beta-mannosidase deficiency associated with peripheral neuropathy". Ann. Neurol. 35 (1): 116-9. doi:10.1002/ana. ...
Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152, endo-beta-mannosidase) is an enzyme. This enzyme catalyses the ... Mannosylglycoprotein+endo-beta-mannosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology ... Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S (September 2004). "Endo-beta-mannosidase, a plant enzyme acting ... "Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium ...
GO:0016985 mannan endo-1,4-beta-mannosidase activity GO:0030246 carbohydrate binding ...
Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein ... Beta-mannosidaseAdd BLAST. 862. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. Length ... "Caprine beta-mannosidase: sequencing and characterization of the cDNA and identification of the molecular defect of caprine ... sp,Q95327,MANBA_CAPHI Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=2 SV=1 ...
What is beta-mannosidase deficiency? Meaning of beta-mannosidase deficiency medical term. What does beta-mannosidase deficiency ... Looking for online definition of beta-mannosidase deficiency in the Medical Dictionary? beta-mannosidase deficiency explanation ... beta-mannosidosis. (redirected from beta-mannosidase deficiency) beta-mannosidosis. n.. See mannosidosis. ... Beta-mannosidase deficiency , definition of beta-mannosidase deficiency by Medical dictionary https://medical-dictionary. ...
beta-mannosidase , mannosidase, beta A, lysosomal , beta-mannosidase-like , hypothetical protein , Beta-mannosidase , lysosomal ... Mannosidase, beta A, Lysosomal (MANBA) ELISA Kit ELISA Kit MANBA Reaktivität: Maus Colorimetric Sandwich ELISA 6.25-400 ng/mL ... Mannosidase, beta A, Lysosomal (MANBA) Antigen-Profil Protein Überblick This gene encodes a member of the glycosyl hydrolase 2 ... Mannosidase, beta A, Lysosomal (MANBA) ELISA Kit ELISA Kit MANBA Reaktivität: Human Colorimetric Competition ELISA 0.78-50 ng/ ...
An inborn error of metabolism marked by a defect in the lysosomal isoform of BETA-MANNOSIDASE that results in lysosomal ... accumulation of mannose-rich intermediate metabolites containing 1,4-beta linkages. The human disease occurs through autosomal ... beta-Mannosidase Deficiency; Deficiency, beta-Mannosidase; beta Mannosidase Deficiency; beta Mannosidosis; beta-Mannosidase ... beta-Mannosidosis (beta-Mannosidase Deficiency). Subscribe to New Research on beta-Mannosidosis ...
BETA-MANNOSIDASE. A, B. 846. Bacteroides thetaiotaomicron VPI-5482. Mutation(s): 0 Gene Names: BT_0458. EC: 3.2.1.25. ... Structural and Biochemical Evidence for a Boat-Like Transition State in Beta-Mannosidases.. Tailford, L.E., Offen, W.A., Smith ... Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. *DOI: 10.2210/pdb2VMF/pdb ... Here we present the analysis of 25 putative beta-mannosidase inhibitors, whose Ki values range from nanomolar to millimolar, on ...
Mannan endo-1,4-beta-mannosidaseImported. ,p>Information which has been imported from another database using automatic ... tr,B8DYX7,B8DYX7_DICTD Mannan endo-1,4-beta-mannosidase OS=Dictyoglomus turgidum (strain Z-1310 / DSM 6724) GN=Dtur_0277 PE=3 ...
Inhibition of Helix pomatia beta-mannosidase using p-nitrophenyl glycoside as substrate assessed as residual activity at 1 mM ...
Purchase high purity recombinant enzyme beta-Mannosidase (Cellulomonas Fimi) for use in research, biochemical enzyme assays and ... One Unit of β-D-mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per ... β-Mannosidases were present in both endosperm and cotyledon-embryo and could be separated chromatographically. The level of ... High purity recombinant β-Mannosidase (Cellilomonas fimi) for use in research, biochemical enzyme assays and in vitro ...
100 μg) Partial protein comprised of amino acids 1 - 86 of the human mannosidase beta A lysosomal-like (MANBAL) protein. ...
Lysosomal beta A mannosidase,MANBA,Mannanase,Mannase \ U0197b for more molecular products just contact us ... CLIA Beta-mannosidase,Bos taurus,Bovine,Lysosomal beta A mannosidase,MANBA,Mannanase,Mannase. Related products : CLIA Beta- ... Product name : CLIA Beta-mannosidase,Bos taurus,Bovine,Lysosomal beta A mannosidase,MANBA,Mannanase,Mannase ... U0197b CLIA Beta-mannosidase,Bos taurus,Bovine,Lysosomal beta A mannosidase,MANBA,Mannanase,Mannase Ask technical file . ...
... beta-galactosidase Subject alpha-mannosidase Remove constraint Subject: alpha-mannosidase Start Over ... Escherichia coli; Orthomyxoviridae; Streptococcus pneumoniae; acetaminophen; alpha-mannosidase; bacteria; beta-galactosidase; ... You searched for: Author Cui, Xikai Remove constraint Author: Cui, Xikai Subject beta-galactosidase Remove constraint ... This approach was used to detect a number of diverse analytes that include enzymes such as β-galactosidase and α-mannosidase ...
MAN2B1: mannosidase alpha class 2B member 1. *MANBA: mannosidase beta. *MAOA: monoamine oxidase A ...
The MANBA gene provides instructions for making the enzyme beta-mannosidase. Learn about this gene and related health ... Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta-mannosidosis. ... Morphological and biochemical studies of human beta-mannosidosis: identification of a novel beta-mannosidase gene mutation. Br ... The mutations result in a beta-mannosidase enzyme with little or no activity, and interfere with the ability of the enzyme to ...
Mannan endo-1,4-beta-mannosidase (IPR016714). *Maltodextrin glucosidase (IPR017069). *Glycoside hydrolase, family 1, beta- ... such as beta-glucosidase) [PMID: 10978344], type II chitinases [PMID: 11846790], 1,4-beta-N-acetylmuraminidases [PMID: 11427528 ... This entry represents the catalytic TIM beta/alpha barrel common to many different families of glycosyl hydrolases found in all ...
4-beta-mannosidase F. Aspergillus clavatus (strain ATCC 1007 / CBS 51365 / DSM 816 / NCTC 3887 /NRRL 1) ... Mannan endo-1,4-beta-mannosidase F UniProtKBInterProSTRINGInteractive Modelling. 436 aa; Sequence (Fasta) ...
Structural and Biochemical Evidence For A Boat-Like Transition State in Beta-Mannosidases ... Bromine in the structure of Structural and Biochemical Evidence For A Boat-Like Transition State in Beta-Mannosidases (pdb 2vqt ... of Bromine atom in the structure of Structural and Biochemical Evidence For A Boat-Like Transition State in Beta-Mannosidases ( ...
Sfla_3816 beta-mannosidase Sfla_1811 glycoside hydrolase family 30 K01186 NEU1; sialidase-1 [EC:3.2.1.18] K01190 lacZ; beta- ... Sfla_0382 Beta-galactosidase Sfla_3951 Beta-N-acetylhexosaminidase Sfla_1061 Beta-N-acetylhexosaminidase Sfla_2388 Glycoside ... beta-mannosidase [EC:3.2.1.25] K01201 GBA; glucosylceramidase [EC:3.2.1.45] ... Sfla_0382 Beta-galactosidase Sfla_1120 putative neuramidase Sfla_0076 Alpha-galactosidase K01634 SGPL1; sphinganine-1-phosphate ...
Substrate Distortion by a Beta-Mannanase: Snapshots of the Michaelis and Covalent-Intermediate Complexes Suggest a B2,5 ... MANNAN ENDO-1,4-BETA-MANNOSIDASE (1GW1:A) * Hydrolase Activity Hydrolyzing O Glycosyl Compounds ... Alpha and beta proteins (a/b) TIM beta/alpha-barrel (Trans)glycosidases beta-glycanases Mannanase A, ManA Pseudomonas cellulosa ...
Mannan endo-1,4-beta-mannosidase. 37288-54-3. From the following sources: Aspergillus niger var, Bacillus lentus, Trichoderma ... beta-carotene. beta-glucanase. 9074-98-0. From the following sources: Aspergillus niger var, Bacillus lentius, B. subtilis, B. ...
3.2.1.77 mannan 1,2-(1,3)-alpha-mannosidase 3.2.1.78 mannan endo-1,4-beta-mannosidase ... 3.2.1.181 galactan endo-beta-1,3-galactanase 3.2.1.182 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside ...
Bacteroides salyersiae GH164 beta-mannosidase 2-deoxy-2-fluoro-beta-D-mannosyl enzyme intermediate ... Bacteroides salyersiae GH164 beta-mannosidase 2-deoxy-2-fluoro-beta-D-mannosyl enzyme intermediate; X-RAY DIFFRACTION 2.55 Å ...
Beta-fructosidase Thermotoga maritima). P49425. (Endo-14-beta-mannosidase Rhodothermus marinus). P06279. (Alpha-amylase ... Exemplary acidic preservatives include, but are not limited to, vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, ...
Synthesis and characterization of new chromogenic substrates for exoglycosidases: \alpha-glucosidase, \alpha-mannosidase, and \ ... beta-galactosidase Authors: DUMITRU PETRU IGA, RICHARD SCHMIDT, SILVIA IGA, CORINA LOREDANA HOTOLEANU, FLORENTINA DUICA, ALINA ... beta-D-galactopyranose was prepared by the glycosylation of this phenol as sodium phenoxide with tetra-O-benzoyl-\alpha-D- ...
lysosomal beta-mannosidase deficiency, see Beta-mannosidosis. *lysosomal glycoaminoacid storage disease-angiokeratoma corporis ... lysosomal beta A mannosidosis, see Beta-mannosidosis. * ... lysosomal alpha-D-mannosidase deficiency, see Alpha- ...
24, 2009, "SubName: Full=Mannan endo-1,4-beta-mannosidase., Cellulase; EC=3.2.1.4; EC=3.2.1.78; Flags: Precursor;", retrieved ... Database UniProt [Online] May 1, 2000, "SubName: Full=Multidomain beta-1,4-beta-mannanase; Flags: Precursor;", retrieved from ... The beta-mannanase enzyme used in conventional enzyme formulations has a temperature maximum of approximately 150 F. Activity ... About 1 gpt of conventional beta-mannanase enzyme, commercially available as GBW-12CD from BJ Services Company diluted in a 1: ...
Overexpression and Purification of Aspergillus aculeatus .BETA.-Mannosidase and Analysis of the Integrated Gene in Aspergillus ... Overexpression and Purification of Aspergillus aculeatus β-Mannosidase and Analysis of the Integrated Gene in Aspergillus ... Overexpression and purification of Aspergillus aculeatus β-mannosidase and analysis of the integrated gene in Aspergillus ...
Common inhibition of both beta-glucosidases and beta-mannosidases by isofagomine lactam reflects different conformational ... Structural and biochemical evidence for a boat-like transition state in beta-mannosidases. ... Understanding how diverse beta-mannanases recognize heterogeneous substrates.. Tailford LE, Ducros VM, Flint JE, Roberts SM, ... Family 6 carbohydrate binding modules recognize the non-reducing end of beta-1,3-linked glucans by presenting a unique ligand ...
  • Beta-mannosidase (EC 3.2.1.25, mannanase, mannase, beta-D-mannosidase, beta-mannoside mannohydrolase, exo-beta-D-mannanase, lysosomal beta A mannosidase) is an enzyme with systematic name beta-D-mannoside mannohydrolase, which is in humans encoded by the MANBA gene. (wikipedia.org)
  • Defects in MANBA, which encodes lysosomal mannosidase, cause beta-mannosidosis. (thefreedictionary.com)
  • The MANBA gene provides instructions for making the enzyme beta-mannosidase. (medlineplus.gov)
  • Approximately 12 mutations that cause beta-mannosidosis have been identified in the MANBA gene. (medlineplus.gov)
  • Beta-mannosidosis is caused by mutations in a gene known as MANBA , which provides instructions for the production of an enzyme called beta-mannosidase. (childrenshospital.org)
  • Genetic mutations in the MANBA gene interfere with the ability of beta-mannosidase to perform its function correctly, leading to an accumulation of complex sugar molecules in cells that eventually causes cells to malfunction. (childrenshospital.org)
  • Identification of two novel beta-mannosidosis-associated sequence variants: biochemical analysis of beta-mannosidase (MANBA) missense mutations. (expasy.org)
  • A recent study in Genetics in Medicine aiming at the identification by whole-exome sequencing of genes involved in infantile nystagmus found heterozygous missense mutations in the MANBA gene, which the encodes lysosomal beta-mannosidase. (ommbidblog.com)
  • Objectives: A novel, high-level expression, thermostable mannan endo-1,4-beta-mannosidase is urgently needed for industrial applications. (ebscohost.com)
  • Results: The mannan endo-1,4-β-mannosidase gene ( MAN) from Aspergillus niger CBS 513.88 was optimized based on the codon usage bias in Pichia pastoris and. (ebscohost.com)
  • A family GH5 (family 5 glycoside hydrolase) (1,4)-beta-D-mannan endohydrolase or beta-D-mannanase (EC 3.2.1.78), designated HvMAN1, has been purified 300-fold from extracts of 10-day-old barley (Hordeum vulgare L.) seedlings using ammonium sulfate fractional precipitation, followed by ion exchange, hydrophobic interaction and size-exclusion chromatography. (edu.au)
  • The barley HvMAN1 gene is a member of a small (1,4)-beta-D-mannan endohydrolase family of at least six genes, and is transcribed at low levels in a number of organs, including the developing endosperm, but also in the basal region of young roots and in leaf tips. (edu.au)
  • A second barley enzyme that participates in mannan depolymerization through its ability to hydrolyse (1,4)-beta-D-manno-oligosaccharides to Man is a family GH1 beta-D-mannosidase, now designated HvbetaMANNOS1, but previously identified as a beta-D-glucosidase [Hrmova, MacGregor, Biely, Stewart and Fincher (1998) J. Biol. (edu.au)
  • 273, 11134-11143], which hydrolyses 4NP (4-nitrophenyl) beta-D-mannoside three times faster than 4NP beta-D-glucoside, and has an action pattern typical of a (1,4)-beta-D-mannan exohydrolase. (edu.au)
  • AGLII and AGLIII were less active on the intact polymer and showed synergy in galacto(gluco)mannan hydrolysis with the mannanase of T. reesei and a beta-mannosidase of Aspergillus niger. (vtt.fi)
  • This enzyme catalyses the following chemical reaction Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides This gene encodes a member of the glycosyl hydrolase 2 family. (wikipedia.org)
  • Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement. (wikipedia.org)
  • Uchino Y, Fukushige T, Yotsumoto S, Hashiguchi T, Taguchi H, Suzuki N, Konohana I, Kanzaki T. Morphological and biochemical studies of human beta-mannosidosis: identification of a novel beta-mannosidase gene mutation. (medlineplus.gov)
  • Mannosidase and Analysis of the Integrated Gene in Aspergillus oryzae. (nii.ac.jp)
  • Brumer, H. Structure-Function Analysis Of A Broad Specificity Populus Trichocarpa Endo-Beta-Glucanase Reveals An Evolutionary Link Between Bacterial Licheninases And Plant Xth Gene Products . (ubc.ca)
  • BACKGROUND & AIMS: Human tumors and liver cancer cell lines express the product of a fusion between the first 13 exons in the mannosidase α class 2A member 1 gene (MAN2A1) and the last 6 exons in the FER tyrosine kinase gene (FER), called MAN2A1-FER. (bireme.br)
  • Here we present the analysis of 25 putative beta-mannosidase inhibitors, whose Ki values range from nanomolar to millimolar, on the Bacteroides thetaiotaomicron beta-mannosidase BtMan2A. (rcsb.org)
  • The invention provides a composition comprising: a) a benefit agent (preferably perfume) delivery particle comprising a poly- xyloglucan or poly-galactomannan with a ratio of beta-1,4 to 1,6 linkages of 1:1 to 3:1, or a mixture thereof as a delivery aid, b) a mannanase, preferably in combination with one or more of lipase, protease and amylase. (sumobrain.com)
  • A composition comprising: a) a benefit agent delivery particle comprising a poly-xyloglucan or poly-galactomannan with a ratio of beta-1 ,4 to 1 ,6 linkages of 1 : 1 to 3: 1 , or a mixture thereof as a delivery aid, b) a mannanase. (sumobrain.com)
  • They acted with three enzymes (beta-mannanase, alpha-mannosidase, and beta-mannosidase) on the biofilms on the surface of hydroxyapatite (the mineral constituent of the teeth). (iansomerhalder.net)
  • The invention also relates to polypeptides having endo-beta-1,4-mannanase activity and polynucleotides encoding the polypeptides. (google.com)
  • Galactomannan structure and β-mannanase and β-mannosidase activity in germinating legume seeds. (megazyme.com)
  • Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. (uniprot.org)
  • Support for a B2,5 transition state during enzymatic beta-mannosidase hydrolysis should also facilitate the design and exploitation of transition state mimics for the inhibition of retaining alpha-mannosidases--an area that is emerging for anticancer therapeutics. (rcsb.org)
  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D- galactosides. (cathdb.info)
  • The enzyme, characterized from multiple bacterial species, catalyzes the hydrolysis of terminal, non-reducing D-mannose residues from beta-1,2-mannotriose and beta-1,2-mannobiose. (expasy.org)
  • Product analyses indicate that transglycosylation reactions occur during hydrolysis of (1,4)-beta-D-manno-oligosaccharides. (edu.au)
  • The D-mannans of Saccharomyces lodderi, Citeromyces matritensis, and Pichia pastoris could also be enzymically degraded to polysaccharides containing predominantly alpha-(1-6)-linked D-mannopyranosyl residues after hydrolysis of most of the beta-D-linked residues in their side chains with acid. (dtic.mil)
  • This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans. (creative-enzymes.com)
  • Definition: Beta-mannosidosis is an autosomal recessive lysosomal storage disease of glycoprotein catabolism caused by a deficiency of lysosomal beta-mannosidase activity. (humpath.com)
  • Overexpression and Purification of Aspergillus aculeatus .BETA. (nii.ac.jp)
  • Gort L, Duque J, Fabeiro JM, Zulaica A, Coll MJ, Chabás A. Molecular analysis in two beta-mannosidosis patients: description of a new adult case. (medlineplus.gov)
  • Molecular analysis in two beta-mannosidosis patients: description of a new adult case. (expasy.org)
  • High purity recombinant β-Mannosidase ( Cellilomonas fimi ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • We prepared various glycosyl donors and acceptors, and recombinant human Golgi endo-α-mannosidase and its various mutants were expressed. (bireme.br)
  • This biochemical analysis of alpha-mannosidase enzyme activity can be used as a 1st tier test for patients with a clinical suspicion of alpha-mannosidosis. (isinproduction.com)
  • Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. (uniprot.org)
  • Activity with beta-1,2-mannotriose or higher oligosaccharides is higher than that with beta-1,2-mannobiose. (expasy.org)
  • Hypersecretion of the alpha-mannosidase precursor by HMW570 was not accompanied by major alterations in N-linked oligosaccharides such as size, charge, and ratio of sulfate and phosphate esters. (rupress.org)
  • Molho-Pessach V, Bargal R, Abramowitz Y, Doviner V, Ingber A, Raas-Rothschild A, Ne'eman Z, Zeigler M, Zlotogorski A. Angiokeratoma corporis diffusum in human beta-mannosidosis: Report of a new case and a novel mutation. (medlineplus.gov)
  • Beta-mannosidase activity in plasma, fibroblasts and leukocytes is minimal. (thefreedictionary.com)
  • deficient in beta-mannosidosis) and nonlysosomal beta-mannosidase (neutral) activity. (curehunter.com)
  • One Unit of β-D-mannosidase activity is defined as the amount of enzyme required to release one µmole of p -nitrophenol per minute from p NP-β-D-mannopyranoside (0.8 mM) in sodium maleate buffer (100 mM), pH 6.5 at 35 o C. (megazyme.com)
  • The mutations result in a beta-mannosidase enzyme with little or no activity, and interfere with the ability of the enzyme to perform its role in breaking down mannose-containing disaccharides. (medlineplus.gov)
  • Homozygous mutant mice have undetectable beta-mannosidase activity. (wikigenes.org)
  • In this study, we examined the transglycosylating activity of a Golgi endo-α-mannosidase. (bireme.br)
  • Based on the findings that TGF-beta activity is elevated in injured RDEB skin, Nystrom and colleagues targeted TGF-beta activity with losartan in RDEB mice. (ommbidblog.com)
  • Beta-mannosidosis mice: a model for the human lysosomal storage disease. (medlineplus.gov)
  • some animal enzymes also hydrolyze beta-D-fucosides and beta-D-glucosides (cf. (cathdb.info)
  • Using a radiolabel pulse-chase protocol, we followed the synthesis and secretion of two of these enzymes, alpha-mannosidase and beta-glucosidase. (rupress.org)
  • Cells exposed to the serine/cysteine proteinase inhibitors leupeptin and antipain and the cysteine proteinase inhibitor benzyloxycarbonyl-L-phenylalanyl-L-alanine-diazomethyl ketone (Z-Phe-AlaCHN2) were unable to complete proteolytic processing of the newly synthesized lysosomal enzymes, alpha-mannosidase and beta-glucosidase. (rupress.org)
  • MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. (antikoerper-online.de)
  • A few hours into the chase period, HMW570 had secreted 95% of its radiolabeled alpha-mannosidase and 86% of its radiolabeled beta-glucosidase as precursor polypeptides compared to the secretion of less than 10% of these forms from wild-type cells. (rupress.org)
  • The deduced amino acid sequence of BXLI shows no similarity with any of the known beta-xylosidases, but is significantly similar to the beta-glucosidases grouped into the family 3 of glycosyl hydrolases. (vtt.fi)
  • Variable clinical presentation of lysosomal beta-mannosidosis in patients with null mutations. (medlineplus.gov)
  • The assay uses 4-methylumbelliferyl substrates to measure the activities of acid sphingomyelinase, alpha-iduronidase, beta-glucosidase, and alpha-mannosidase. (ggc.org)
  • Neither alpha-mannosidase nor beta-glucosidase in HMW570 were ever found in the lysosomal fractions of sucrose gradients consistent with HMW570 being defective in lysosomal enzyme targeting. (rupress.org)
  • Also, both alpha-mannosidase and beta-glucosidase precursors in the mutant strain were membrane associated as previously observed for wild-type precursors, indicating membrane association is not sufficient for lysosomal enzyme targeting. (rupress.org)
  • Antipain and leupeptin treatment resulted in both a dramatic decrease in the efficiency of proteolytic processing, as well as a sevenfold increase in the secretion of alpha-mannosidase and beta-glucosidase precursors. (rupress.org)
  • The structure of mammalian beta-mannosidase provides insight into beta-mannosidosis and nystagmus. (expasy.org)
  • Endo-α-Mannosidase-Catalyzed Transglycosylation. (bireme.br)
  • An inborn error of metabolism marked by a defect in the lysosomal isoform of BETA-MANNOSIDASE that results in lysosomal accumulation of mannose-rich intermediate metabolites containing 1,4-beta linkages. (curehunter.com)
  • These disaccharides gradually accumulate in the lysosomes and cause cells to malfunction, resulting in the signs and symptoms of beta-mannosidosis. (medlineplus.gov)
  • What are the symptoms of beta-mannosidosis? (childrenshospital.org)
  • Beta,beta-difluorinated amino acid derivatives were synthesized via Mg(0)-promoted defluorination of alpha-trifluoromethyl iminoester. (biomedsearch.com)
  • Pd-catalyzed asymmetric hydrogenation of the bromodifluoromethyl iminoester and the subsequent transformations provided optically active beta,beta-difluoroglutamic acid and beta,beta-difluoroproline derivatives. (biomedsearch.com)
  • The antioxidant and antiradical activities of 5,7,3'-trihydroxy-3,6,4'-trimethoxyflavone or centaureidin isolated and characterized from Brickellia veronicaefolia were elucidated by heat-induced oxidation in a beta-carotene and linoleic acid system and by the 1,1-diphenyl-2-picrylhydrazyl decoloration test. (biomedsearch.com)
  • Analysis of organic acids after incubation with (16-2H3)palmitic acid in fibroblasts from patients with mitochondrial beta-oxidation defects. (thefreedictionary.com)
  • The therapeutic effect of EAS on PD may involve in regulating the tyrosine metabolism, mitochondrial beta-oxidation of long chain saturated fatty acids, fatty acid metabolism, methionine metabolism, and sphingolipid metabolism. (thefreedictionary.com)
  • Diminished carnitine reserves in muscle of obese rats was accompanied by marked perturbations in mitochondrial fuel metabolism, including low rates of complete fatty acid oxidation, elevated incomplete beta-oxidation , and impaired substrate switching from fatty acid to pyruvate. (thefreedictionary.com)
  • Mitochondrial long chain fatty acid beta-oxidation in man and mouse. (thefreedictionary.com)