A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
An enzyme that catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-galactosides including galactose oligosaccharides, galactomannans, and galactolipids.
A 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by COMPLEMENT FACTOR I into C3c (749-954), and C3dg (955-1303) in the presence COMPLEMENT FACTOR H.
Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.
A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)
Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
A surgical specialty concerned with the study and treatment of disorders of the ear, nose, and throat.
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)
Vaccines used in conjunction with diagnostic tests to differentiate vaccinated animals from carrier animals. Marker vaccines can be either a subunit or a gene-deleted vaccine.
The inactive form of GLYCOGEN PHOSPHORYLASE that is converted to the active form PHOSPHORYLASE A via phosphorylation by PHOSPHORYLASE KINASE and ATP.
The sum of the weight of all the atoms in a molecule.
A class of glucosyltransferases that catalyzes the degradation of storage polysaccharides, such as glucose polymers, by phosphorolysis in animals (GLYCOGEN PHOSPHORYLASE) and in plants (STARCH PHOSPHORYLASE).
The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Includes ortho-, meta-, and para-nitrophenylgalactosides.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Method of making images on a sensitized surface by exposure to light or other radiant energy.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.

Fecal coliform elevated-temperature test: a physiological basis. (1/7076)

The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.5 degrees C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5 degrees C. A temperature increase from 35 to 44.5 degrees C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform beta-galactosidase activity coupled with thermal inactivation of this enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.  (+info)

The effects of digestive enzymes on characteristics of placental insulin receptor. Comparison of particulate and soluble receptor preparations. (2/7076)

The role of the surrounding membrane structure on the binding characteristics of the insulin receptor was studied by using several digestive enzymes. The effects observed with particulate membrane preparations are compared with those from soluble receptor preparations. beta-Galactosidase and neuraminidase had no effect on insulin binding to either particulate or soluble receptors from human placentae. Exposure to 2 units of phospholipase C/ml increased insulin binding to particulate membranes, but was without effect on the soluble receptor preparation. The increase in binding to particulate membranes was shown to be due to an increase in apparent receptor number. After 5 min exposure to 500 microgram of trypsin/ml there was an increase in insulin binding to the particulate membrane fraction, owing to an increase in receptor affinity. After 15 min exposure to this amount of trypsin, binding decreased, owing to a progressive decrease in receptor availability. In contrast, this concentration of trypsin had no effect on the solubilized receptor preparation. Because of the differential effects of phospholipase C and trypsin on the particulate compared with the solubilized receptor preparations, it is concluded that the effects of these enzymes were due to an effect on the surrounding membrane structure. Changes in receptor configuration due to alterations within the adjoining membrane provide a potential mechanism for mediating short-term alterations in receptor function.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (3/7076)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (4/7076)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Pathogenicity island 2 mutants of Salmonella typhimurium are efficient carriers for heterologous antigens and enable modulation of immune responses. (5/7076)

The potential use as vaccine delivery system of Salmonella typhimurium strains harboring defined mutations in the sseC (HH104) and sseD (MvP101) genes, which encode putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2, was evaluated and compared with that of the well-characterized aroA mutant strain SL7207 by using beta-galactosidase (beta-Gal) as a model antigen. When orally administered to immune-competent or gamma interferon-deficient (IFN-gamma-/-) BALB/c mice, both mutants were found to be highly attenuated (50% lethal dose, >10(9) bacteria). Both strains were also able to efficiently colonize and persist in Peyer's patches. Immunization with HH104 and MvP101 triggered beta-Gal-specific serum and mucosal antibody responses equivalent to or stronger than those observed in SL7207-immunized mice. Although immunoglobulin G2 (IgG2) serum antibodies were dominant in all groups, IgG1 was also significantly increased in mice vaccinated with MvP101 and SL7207. Comparable beta-Gal-specific IgA and IgG antibodies were detected in intestinal lavages from mice immunized with the different strains. Antigen-specific CD4(+) T-helper cells were generated after vaccination with all vaccine prototypes; however, responses were significantly more efficient when HH104 and MvP101 were used (P < 0.05). Significantly higher levels of IFN-gamma were produced by restimulated spleen cells from mice immunized with HH104 than from those vaccinated with the MvP101 or SL7207 derivatives (P +info)

Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes. (6/7076)

BACKGROUND: In congestive heart failure, high systemic levels of the hormone arginine vasopressin (AVP) result in vasoconstriction and reduced cardiac contractility. These effects are mediated by the V1 vasopressin receptor (V1R) coupled to phospholipase C beta-isoforms. The V2 vasopressin receptor (V2R), which promotes activation of the Gs/adenylyl cyclase system, is physiologically expressed in the kidney but not in the myocardium. Expression of a recombinant V2R (rV2R) in the myocardium could result in a positive inotropic effect via the endogenous high concentrations of AVP in heart failure. METHODS AND RESULTS: A recombinant adenovirus encoding the human V2R (Ad-V2R) was tested for its ability to modulate the cardiac Gs/adenylyl cyclase system and to potentiate contractile force in rat ventricular cardiomyocytes and in H9c2 cardiomyoblasts. Ad-V2R infection resulted in a virus concentration-dependent expression of the transgene and led to a marked increase in cAMP formation in rV2R-expressing cardiomyocytes after exposure to AVP. Single-cell shortening measurements showed a significant agonist-induced contraction amplitude enhancement, which was blocked by the V2R antagonist, SR 121463A. Pretreatment of Ad-V2R-infected cardiomyocytes with AVP led to desensitization of the rV2R after short-term agonist exposure but did not lead to further loss of receptor function or density after long-term agonist incubation, thus demonstrating resistance of the rV2R to downregulation. CONCLUSIONS: Adenoviral gene transfer of the V2R in cardiomyocytes can modulate the endogenous adenylyl cyclase-signal transduction cascade and can potentiate contraction amplitude in cardiomyocytes. Heterologous expression of cAMP-forming receptors in the myocardium could lead to novel strategies in congestive heart failure by bypassing the desensitized beta-adrenergic receptor signaling.  (+info)

Multiple cis-acting regulatory regions are required for restricted spatio-temporal Hoxa5 gene expression. (7/7076)

Genetic analyses have revealed the essential role of the murine Hoxa5 gene for the correct specification of the cervical and upper thoracic region of the skeleton, and for the normal organogenesis and function of the respiratory tract, both structures expressing Hoxa5 during embryogenesis. To understand how the expression domains of the Hoxa5 gene are established during development, we have analyzed the cis-acting control regions mediating Hoxa5 gene expression using a transgenic approach. Four transcripts are derived from the Hoxa5 locus. The shortest and most abundant one displays a specific spatio-temporal profile of expression at earlier stages and in more anterior structures along the embryonic axis than the larger forms. We established that an 11.1 kilobase pair (kb) genomic fragment, extending from position -3.8 kb to +7.3 kb relative to Hoxa5 transcription initiation site, was sufficient to reproduce the temporal expression and substantially reconstitute the spatial pattern of the major Hoxa5 transcript. By deletion analyses, we identified a 2.1 kb fragment located downstream of the Hoxa5 gene that possesses mesodermal enhancer activity. Overall, the findings demonstrate that cis-acting regulatory elements essential for the correct expression of the major Hoxa5 transcript are located both upstream and downstream of the Hoxa5 coding sequences.  (+info)

Ectopic expression of the transforming growth factor beta type II receptor disrupts mesoderm organisation during mouse gastrulation. (8/7076)

Transforming growth factor beta (TGFbeta) regulates the cell cycle and extracellular matrix (ECM) deposition of many cells in vitro. We have analysed chimaeric mouse embryos generated from embryonic stem cells with abnormal receptor expression to study the effect of TGFbeta on these processes in vivo and the consequences for normal development. The binding receptor for TGFbeta, TbetaRII, is first detected in the embryo proper around day 8.5 in the heart. Ectopic expression of TbetaRII from the blastocyst stage onward resulted in an embryonic lethal around 9.5 dpc. Analysis of earlier stages revealed that the primitive streak of TbetaRII chimaeras failed to elongate. Furthermore, although cells passed through the streak and initially formed mesoderm, they tended to accumulate within the streak. These defects temporally and spatially paralleled the expression of the TGFbeta type I receptor, which is first expressed in the node and primitive streak. We present evidence that classical TGFbeta-induced growth inhibition was probably the cause of insufficient mesoderm being available for paraxial and axial structures. The results demonstrate that (1) TGFbeta mRNA and protein detected previously in early postimplantation embryos is present as a biologically active ligand; and (2) assuming that ectopic expression of TbetaRII results in no other changes in ES cells, the absence of TbetaRII is the principle reason why the embryo proper is unresponsive to TGFbeta ligand until after gastrulation.  (+info)

Beta Galactosidase | Learn more about Beta Galactosidase | Meaning of Beta Galactosidase | Description of Beta Galactosidase | Details of Beta Galactosidase | Article on Beta Galactosidase | Essay on Beta Galactosidase | Definition of Beta Galactosidase | Infospaze
microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Quantitation of β-galactosidase activity. In yeast cells, co-transformed with pGADT7 (AD) and pGBKT7 (BD) constructs as indicated, β-galactosidase activity wa
Buy our Recombinant |em|E. coli |/em| beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols…
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
BioTek Notes dapplication, 26-Feb-07, Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
|strong|Rabbit anti beta-galactosidase polyclonal antibody|/strong| detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccha…
BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN
a The β-galactosidase activity assay was performed as described in the MATCHMAKER two-hybrid system (20). Briefly, cells from 1.5-ml samples of exponential culture were collected by centrifugation and resuspended in 300 μl of Z-buffer (20). A 100-μl aliquot of the resuspended cells was lysed by quick freeze-thaw (treatment with liquid nitrogen followed by thawing at 37°C). To measure the β-galactosidase activity in the cell lysate, a 0.7-ml sample of the Z-buffer-β-mercaptoethanol solution was added to each tube followed by 0.16 ml of Z-buffer-ONPG (4 mg of ONPG per 1 ml of Z-buffer). The time of ONPG addition was recorded, and the tubes were incubated at 30°C with shaking. When yellow color was visible, 400 μl of 1 M NaCO3 was added to each tube to terminate the reaction, and the time was recorded. The tubes were then centrifuged for 10 min at 10,000 × g to remove cellular debris, and the optical density at 420 nm was recorded. β-Galactosidase units were defined as the amount of ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.) ...
METHODS AND RESULTS The method involves the harvest of autologous venous-derived endothelial cells, the efficient genetic modification of the cells through the use of recombinant retroviruses, and the subsequent implantation of the genetically modified cells on the surface of balloon-denuded arterial segments. With a rabbit model, freshly isolated endothelial cells were transduced with a recombinant retrovirus encoding the bacterial enzyme beta-galactosidase. The autologous transduced cells were then implanted on the surface of balloon-denuded ileofemoral arterial segments at different cell densities; after 1 to 14 days, the animals were killed, and the vessel segments were examined. Cells expressing the bacterial gene product, as determined by in situ staining for beta-galactosidase, were found to be present on the surface of 28 of the 32 arteries seeded with genetically modified cells. Vessels examined at 4 to 7 days after seeding displayed 40% to 90% coverage with transduced cells, even when ...
Beta-galactosidase, 0.1 ml. This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates.
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic units (monosaccharides). - Stock Image C015/1970
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade Quantity:
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
The cae8 promoter is sensitive to the amino acid L-asparagine.The figure shows measured β-galactosidase activities from wild-type BW25113 cells, carrying the c
Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C
C-terminal BetaGal reporter tag yeast expression plasmid. This vector allows the creation of reporter fusion proteins with no protease cleavage tag.
lacZ. No transcription. Structural genes lacZ. lacY. lac A. No transcription. Presence of lactose. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor). Allolactose. 12 When lactose is present, some of it is converted into allolactose,.... RNA polymerase lacO operator. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor) ...
BioAssay record AID 404864 submitted by ChEMBL: Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene assay relative to control.
Old cells, new tricks: chromatin structure in senescence | SpringerLink. Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) | Abcam. Overview of Cellular Senescence and Aging | Cell Signaling Technology. The chemistry of senescence | Nature Reviews Chemistry. Infographic: How Does Cell Senescence Drive Aging and Disease? | The Scientist Magazine®. Cellular senescence is associated with age-related blood clots. Frontiers | Cellular Senescence as the Causal Nexus of Aging | Genetics. Diabetic Retinopathy: premature cellular senescence and microvascular dysfunction - ELISA Genie. Stem Cells Versus Senescence | JACC: Journal of the American College of Cardiology. MHY2233 Attenuates Replicative Cellular Senescence in Human Endothelial Progenitor Cells via SIRT1
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase ...
Substâncias complexas são convertidas, pela ação de enzimas, em moléculas solúveis, durante o processo de germinação, as quais são translocadas para a plântula em crescimento, servindo como fonte de energia ou estrutura física. Com o objetivo de quantificar a atividade enzimática da -galactosidase e a mobilização de mono e oligossacarídeos durante o período de germinação, foi conduzido este estudo utilizando-se sementes de jacarandá-da-bahia. As sementes foram mantidas em germinador a 25oC sob luz contínua, sendo avaliada a protrusão da radícula, pelo período de 10 dias. Foram quantificados os teores de mono e de oligossacarídeos, assim como a atividade específica da enzima -galactosidase nos tempos zero, um, três e cinco dias. Houve mobilização das reservas de glicose e manose nos cotilédones e xilose neste e no embrião nos três primeiros dias de germinação. A ramnose teve os teores aumentados nos cotilédones e no eixo embrionário. A rafinose foi o ...
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
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You might also use the Kyoto Encyclopedia of Genes and Genomes, which has a lot of information conveniently summarized for many proteins; for example, the entry for the E. coli beta-galactosidase gene is here. Note that the entries for orthologies, pathways, motifs, cross-references to other databases, structures, etc. are all clickable.. ...
It was found that Concanavalin A (Con A) accelerates the rates of hydrolysis of E. coli beta-galactosidase and yeast invertase by binding to the product (glucose) formed in the reaction. The effect of Con A can be made ...
The adsorption of beta-galactosidase (BG) from solution by anti-beta-galactosidase monoclonal antibody (ABG) immobilized on nonporous, glass coated beads has been studied in a stirred batch adsorber at 4, 8, 15, and 20°C and for different initial BG concentrations. The elution of BG from the BG-ABG complex was also examined. The experiments performed in this work provided data from which information may be obtained about the influence of the concentration of BG and of the temperature on the dynamics of the adsorption of BG onto immobilized ABG, as well as on the total amount of BG adsorbed at equilibrium. Such experimental data may be used in studies involving the modelling of the kinetics of adsorption of BG onto immobilized ABG--Abstract, page ii.
An optimized chemiluminescent assay for beta-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2-tricyclo-[3.3.1. 1(3,7)]decan]-4- yl)phenyl-beta-D-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of beta-gal …
BioAssay record AID 142597 submitted by ChEMBL: Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in NIH 3T3 cells. Maximal effect normalized to the effect of carbachol (%CCH)..
Interestingly, elimination of PA5471.1 translation via an M1T mutation also enhanced PA5471 expression, reminiscent of the effects of eliminating the pBC16 tet LP ribosome binding site (i.e., no translation of the tet gene possible) where Tet protein expression was enhanced 10-fold and was tetracycline unresponsive (29). It is unclear, however, how lack of PA5471.1 translation would provide the same signal as ribosome stalling, although in the case of the M1T mutation the ribosomes may be pausing at the ribosome binding site in the absence of a start codon.. The observation that fusion strains carrying 3′-truncated fragments of the PA5471 upstream region lacking a putative attenuator (Fig. 1, K2809, K2792, and K2794) yielded less β-galactosidase activity than full-length upstream fragments containing the attenuator sequences but carrying a nonsense mutation (Q3Am) in PA5471.1 (Fig. 1, K2800) was puzzling given the expectation that an attenuator-minus sequence would, in theory, provide for ...
The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert presence of insert results into insertional inactivation of Beta galactosidase and recombinant colonies do not produce any colour these are identified as Recombinant Colony
Cellular senescence is a fundamental cell fate playing significant and complex roles during tumorigenesis and natural aging process. However, the molecular determinants distinguishing senescence from other temporary and permanent cell-cycle arrest states such as quiescence and post-mitotic state and the specified mechanisms underlying cell-fate decisions towards senescence versus cell death in response to cellular stress stimuli remain less understood. In our studies, we aimed to employ multi-omics approaches to deepen our understanding of cellular senescence, in particular, regarding the specific molecular determinants distinguishing cellular senescence from other non-dividing cell fates. Notably, one of the most prominent features of cellular senescence differing from other non-dividing cell fates is the increased expression of senescence-associated beta-galactosidase. Because 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG) is known as the substrate catalyzed by ...
A Q: for transfection gurus. We are measuring promoter activity using luciferase gene as a main reporter, fused to the putative promoter fragments, and CMV/beta-galactosidase gene as a second, normalizer activity. Both are introduced by lipofection into an aortic cell line. Luciferase activity is measured using a Promega kit, and the b-gal is measured via a chemiluminescence based kit from Tropix (GalactoLight). Before measuring the latter activity we heat-inactivate the endogenous activity. What we get is that for a given construct the luciferase activity in a 3X transfection series is relatively constant ( within a factor of 1.5), but the respective set of b-gal activities is variable within a factor of 3, which ruins the experiment. Any ideas? Has anyone used GalactoLight with success? -- Alexander Kraev, PhD Biochemie III, ETHZ Zurich Phone 41-1-632-31-47 Fax 41-1-632-12-13 e-mail kraev at bc.biol.ethz.ch ...
Using a fungus two-hybrid system, we isolated a book human centrosomal protein, CPAP (centrosomal P4. utilized to display screen for protein that connect to 4.1R-135. The top domains (HD; residues 1 to 209) of 4.1R-135 (4.1R-HD) was fused towards the GAL4 DNA-binding domains (GAL4-DB) in vector pAS2-1 (Clontech). This create was used as bait to display a Z-VAD-FMK inhibition human being lymphocyte cDNA library fused to a GAL4 activation website (GAL4-AD) in the pACT2 vector (Clontech). The two types of plasmids were then cotransformed into Y190, and the transformants were selected on SD minimal medium as previously explained (40). Positive colonies were further tested for -galactosidase activity using a colony-lift assay and liquid assay as explained by the manufacturer (Clontech). To thin down the head website region of 4.1R (4.1R-HD) that binds to CPAP, constructs containing numerous portions of 4.1R-HD were fused to GAL4-DB of the pAS2-1 vector (Fig. ?(Fig.1A).1A). The C terminus of CPAP ...
Dimerization data (column 1) represent relative binding to GST-TBP(181C) as determined in Figure 1E and are an average of six sets of data. See Figure 5 for standard errors. β-galactosidase activity (columns 3-6) is relative to wild-type test TBP in column 3 (1.0 = 3 Miller units using the high-sensitivity substrate CPRG) and represents an average of at least three independent determinations. All data were obtained in the linear range of the assay. The null allele expresses only the first 81 amino acids of TBP. Test HA-tagged TBP levels were determined by quantitative Western blotting with TBP antibodies as described in Figure 5B. TBP levels are relative to the endogenous (untagged) TBP (1 = 17,000 ± 2,000 molecules per cell). The standard error for all data is presented graphically in Figure 5E. Expression of the TBPEBmutants did not cause a decrease in the expression of TAFII145 (data not shown). Low in columns 3 and 7 indicates that the test TBP was driven by the TBP promoter; high in ...
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The results of this study are consistent with the following conclusions. First, unconjugated β-galactosidase is rapidly cleared from blood in vivo (Table 2), owing to rapid uptake of the unconjugated enzyme by liver and spleen (Figs. 2 and 3). Second, once inside cells, β-galactosidase is rapidly degraded in vivo such that 99% of the organ enzyme activity is lost at 4 h after an intravenous injection (Table 1). Third, the 116-kDa β-galactosidase (Fig. 1B) can be conjugated to the 8D3 TfRmAb without loss of enzyme activity (Fig. 1C). Fourth, there is minimal brain uptake of the unconjugated β-galactosidase, but there is a 10-fold increase in brain uptake of enzyme following conjugation to the 8D3 TfRmAb (Table 1; Figs. 2 and 3).. The β-galactosidase is rapidly removed from the blood due to the avid uptake of the enzyme by liver and spleen (Figs. 2 and 3), which confirms the earlier observation of Onodera et al. (1983). The blood concentration of the β-galactosidase-TfRmAb is 5- to 10-fold ...
ATCC ® 77267™ Designation: pUN65 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
ATCC ® 77269™ Designation: pUN90 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Edvotek 300 kit shows how to clone a DNA fragment by using ligation, transformation and an assay of ß-galactosidase. For advanced students.
Page contains details about β-galactosidase/ZIF-8 MOF coating . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Expresses the head domain of Nod fused to the coiled-coil domain of KHC and beta-galactosidase under the control of UAS; the fusion protein accumulates at the minus ends of microtubules, N.G ...
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols. ...
reacts with native and denatured-reduced E. coli β-galactosidase(116 kD); may be usedfor detection of β-galactosidase expressed by E. colilacZ gene encoded in many cloned gene sequences,and serves as an indicator for fusion proteins encodedby an inserted DNA ...
Autosomal recessive disorder; beta-galactosidase deficiency; neuronal storage of GM1 ganglioside and visceral storage of ... Most patients have a normal size liver and spleen.Prenatal diagnosis is possible by measurement of Acid Beta Galactosidase in ... The GM1 gangliosidoses are caused by a deficiency of beta-galactosidase, with resulting abnormal storage of acidic lipid ... Mutations with higher residual beta-galactosidase activity for the GM1 substrate than for keratan sulfate and other galactose- ...
... beta-galactosidase (EC 3.2.1.23); 6-phospho-beta-galactosidase (EC 3.2.1.85); 6-phospho-beta-glucosidase (EC 3.2.1.86); lactase ... 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT; LCTL; GH1 in CAZypedia Henrissat B, Callebaut I, Fabrega S ... Glycoside hydrolase family 1 CAZY GH_1 comprises enzymes with a number of known activities; beta-glucosidase (EC 3.2.1.21); ... hydrolase (EC 3.2.1.62), lactase (EC 3.2.1.108); beta-mannosidase (EC 3.2.1.25); myrosinase (EC 3.2.1.147). ...
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ... Rhodanobacter fulvus produce beta-galactosidase. Parte, A.C. "Rhodanobacter". LPSN. "Rhodanobacter fulvus". www.uniprot.org. ...
... endo-beta-1,3-glucanase (EC 3.2.1.39); endo-beta-1,3-1,4-glucanase (EC 3.2.1.6); endo-beta-galactosidase (EC 3.2.1.103). ...
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ...
"Entrez Gene: Galactosidase, beta 1-like 3". Retrieved 2012-07-24. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, ... Galactosidase, beta 1-like 3 is a protein in humans that is encoded by the GLB1L3 gene. GRCh38: Ensembl release 89: ...
weissbach, H (1977). "DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors". The Journal of ...
The bacterial lacZ gene encodes a beta-galactosidase enzyme. When media containing certain galactosides (e.g. X-gal), cells ...
1991). "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases". ... 1994). "Normal serum beta-galactosidase in juvenile GM1 gangliosidosis". Pediatr. Neurol. 10 (4): 317-9. doi:10.1016/0887-8994( ... Chakraborty S, Rafi MA, Wenger DA (1994). "Mutations in the lysosomal beta-galactosidase gene that cause the adult form of GM1 ... 1991). "Combined deficiency of beta-galactosidase and neuraminidase: natural history of the disease in the first 18 years of an ...
... beta-galactosidase (EC 3.2.1.23); beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a ... The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... Jacobson RH, Zhang XJ, DuBose RF, Matthews BW (June 1994). "Three-dimensional structure of beta-galactosidase from E. coli". ... beta-galactosidase from Escherichia coli". The Journal of Biological Chemistry. 267 (16): 11126-30. PMID 1350782. ...
von Degenfeld, Georges; Wehrman, Tom S.; Blau, Helen M. (2009-01-01). Imaging beta-galactosidase activity in vivo using ... "Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence". Nature ... Her discovery of β-galactosidase complementation is widely used in drug discovery. Non-invasive bioluminescence imaging enables ...
This leads to a deficiency of beta-galactosidase (GLB1) and neuraminidase-1 (NEU1). As a result, the lysosome is unable to ... This gene encodes the enzyme cathepsin A, which forms a protein complex with neuraminidase-1 and beta-galactosidase to break ... Cathepsin A is required in this process because within the complex, it prevents neuraminidase-1 and beta-galactosidase from ... in 1979 by measuring beta-galactosidase and neuraminidase activities in cultured amniotic fluid cells. Currently, there is no ...
ATCC Flood, M. T.; Kondo, M (2004). "Toxicity evaluation of a beta-galactosidase preparation produced by Penicillium multicolor ... "Isolation and characterization of a beta-primeverosidase-like enzyme from Penicillium multicolor". Bioscience, Biotechnology, ...
In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter ... Activation, stabilization and association with beta-galactosidase and its protective protein". Eur. J. Biochem. 149 (2): 315-21 ... "Association of N-acetylgalactosamine-6-sulfate sulfatase with the multienzyme lysosomal complex of beta-galactosidase, ...
"Molecular basis of beta-galactosidase alpha-complementation". Proceedings of the National Academy of Sciences of the United ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ...
Beta-galactosidase PDBe-KB provides an overview of all the structure information available in the PDB for Escherichia coli Beta ... beta-galactosidase". Biochemistry. 40 (49): 14781-94. doi:10.1021/bi011727i. PMID 11732897. Fernandez P, Cañada FJ, Jiménez- ... Lactase (also known as lactase-phlorizin hydrolase, or LPH), a part of the β-galactosidase family of enzymes, is a glycoside ... The Lactase Protein E. coli β-galactosidase: PDB: 1JYY​ Gene Ontology for Lactase Making of the Fittest: Got Lactase? The Co- ...
Olson and Eglen; Eglen, RM (2007). "beta Galactosidase complementation: A cell-based luminescent assay platform for drug ...
... beta-galactosidase) Luciferase, including ReBiL (recombinase enhanced bimolecular luciferase) and Gaussia princeps luciferase. ... "Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation". Proceedings of the ... Park JH, Back JH, Hahm SH, Shim HY, Park MJ, Ko SI, Han YS (October 2007). "Bacterial beta-lactamase fragmentation ... Remy I, Ghaddar G, Michnick SW (2007). "Using the beta-lactamase protein-fragment complementation assay to probe dynamic ...
Ullmann, A.; Jacob, F.; Monod, J. (1968). "On the subunit structure of wild-type versus complemented beta-galactosidase of ... "The role of the inducible alleles and the constitutive alleles in the synthesis of beta-galactosidase in zygotes of Escherichia ... of a peptide fraction of Escherichia coli beta-galactosidase". Journal of Molecular Biology. 12 (3): 918-923. doi:10.1016/S0022 ... by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural ...
"Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol". J. Immunol. ...
"Analysis of synphilin-1 and synuclein interactions by yeast two-hybrid beta-galactosidase liquid assay". Neurosci. Lett. 325 (2 ... "Analysis of synphilin-1 and synuclein interactions by yeast two-hybrid beta-galactosidase liquid assay". Neurosci. Lett. 325 (2 ...
5 minutes for beta-galactosidase[citation needed]). Prokaryotic small ribosomal subunit (30S) Ribosomal RNA 23S methyl RNA ...
A point mutation in the lacZ gene alters beta-galactosidase activity producing a uniform populations of beta-galactosidase ... Rotman, M B; Celada, F (June 1, 1968). "Antibody-mediated activation of a defective beta-D-galactosidase extracted from an ... In 1961, Rotman developed a system capable of measuring the enzymatic activity of individual molecules of beta-galactosidase ... partial inactivation of beta-galactosidase resulting from storing crystalline enzyme under ammonium sulfate at low temperature ...
The glycosyl hydrolase 42 family CAZY GH_42 comprises beta-galactosidase enzymes (EC 3.2.1.23). These enzyme catalyse the ... beta-galactosidase gene (pbg) is located in the 3'-flanking region". Microbiology and Immunology. 39 (9): 677-86. doi:10.1111/j ... hydrolysis of terminal, non-reducing terminal beta-D-galactoside residues. The middle domain of these three-domain enzymes is ...
"Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing beta-galactosidase." ...
Matsumura, I; Ellington, AD (Jan 12, 2001). "In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds ...
... and stain positive for senescence-associated β-galactosidase activity. Two proteins, senescence-associated beta-galactosidase ... cells that naturally have these two proteins such as maturing tissue macrophages with senescence-associated beta-galactosidase ... and stain positive for senescence-associated β-galactosidase activity. The nucleus of senescent cells is characterized by ... Ras showed signs of senescence following p53 reactivation including an increase in senescence associated B-galactosidase ...
"Analysis of synphilin-1 and synuclein interactions by yeast two-hybrid beta-galactosidase liquid assay". Neuroscience Letters. ... Beta amyloid Synuclein Contursi Terme - the village in Italy where a mutation in the α-synuclein gene led to a family history ... A single molecule study in 2008 suggests alpha-synuclein exists as a mix of unstructured, alpha-helix, and beta-sheet-rich ... There is evidence of a structured intermediate rich in beta structure that can be the precursor of aggregation and, ultimately ...
... modeling beta-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta (BBA) - General Subjects. 1770 (5): 733- ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
Galactosidases *Alpha. *Beta. *alpha-Mannosidase. *Glucuronidase *Klotho. *Hyaluronidase. *Pullulanase. *Glucosylceramidase * ...
Mutations in TGFBI which encodes transforming growth factor beta induced cause several forms of corneal dystrophies including ... males and asymptomatic female carriers of an X-linked systemic metabolic disease caused by a deficiency of α-galactosidase, ...
... beta-D-glucosidase, beta-glucoside glucohydrolase, arbutinase, amygdalinase, p-nitrophenyl beta-glucosidase, primeverosidase, ... Beta-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and ... Vicianin beta-glucosidase. References[edit]. *^ PDB: 3AHX​; Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang ... amygdalase, linamarase, salicilinase, and beta-1,6-glucosidase. Cellulose is a polymer composed of beta-1,4-linked glucosyl ...
Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase- ...
... beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase), ... Separation and characterization of cellulases and beta-glucosidases". The Biochemical Journal. 177 (1): 9-19. doi:10.1042/ ... The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ...
Galactosidases *Alpha. *Beta. *alpha-Mannosidase. *Glucuronidase *Klotho. *Hyaluronidase. *Pullulanase. *Glucosylceramidase * ...
Earliest discoveries of transferase activity occurred in other classifications of enzymes, including Beta-galactosidase, ...
One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved ...
Beta ketothiolase deficiency. *Beta-galactosidase-1 deficiency. *Beta-mannosidosis. *Beta-sarcoglycanopathy. *Beta-thalassemia ...
In the beta form, the alcohol group is in the equatorial position, whereas in the alpha form, the alcohol group is in the axial ... The hydrolysis of lactose to glucose and galactose is catalyzed by the enzymes lactase and β-galactosidase. The latter is ... In the cyclic form there are two anomers, named alpha and beta, since the transition from the open-chain form to the cyclic ...
Beta-galactosidase (EC 3.2.1.23). *Hyaluronidase (EC 3.2.1.35). Category:EC 3.3 (act on ether bonds)Edit. *Category:EC 3.3 ... EC 6.2.1.28: 3-alpha,7-alpha-dihydroxy-5-beta-cholestanate--CoA ligase ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
Modeling β-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta (BBA) - General Subjects. 1770 (5): 733-746 ...
In S-glycosylation, a beta-GlcNAc is attached to the sulfur atom of a cysteine residue.[4] ... Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
... β-galactosidase.[78] Significant results have been obtained by some companies to extend mouse lifespan focusing on senescent ... "Age-Related Loss of Innate Immune Antimicrobial Function Of Dermal Fat Is Mediated By Transforming Growth Factor Beta" ... "Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young ...
Hengstenberg, W., Penberthy, W.K. and Morse, M.L. (1970). "Purification of the staphylococcal 6-phospho-β-D-galactosidase". Eur ... 6-Fosfo-beta-galaktozidaza (EC 3.2.1.85, fosfo-beta-galaktozidaza, beta-D-fosfogalaktozid galaktohidrolaza, fosfo-beta-D- ... galaktozidaza, 6-fosfo-beta-D-galaktozidaza) je enzim sa sistematskim imenom 6-fosfo-beta-D-galaktozid 6-fosfogalaktohidrolaza. ... 6-fosfo-beta-D-galaktozid + H2O ⇌. {\displaystyle \rightleftharpoons }. 6-fosfo-D-galaktoza + alkohol. Reference[uredi - уреди ...
... beta-h-fructosidase, beta-fructosidase, invertin, sucrase, maxinvert L 1000, fructosylinvertase, alkaline invertase, acid ... invertase, and the systematic name: beta-fructofuranosidase. The resulting mixture of fructose and glucose is called inverted ...
Beta-galactosidase. *Beta-glucosidase. *Beta-ketoacyl-ACP synthase. *Beta-secretase. *Betaine-homocysteine S-methyltransferase ...
alpha-galactosidase (Agalsidase alfa. *Agalsidase beta). *Glycosaminoglycan: iduronidase (Laronidase). *arylsulfatase B ( ... A16AB04 Agalsidase beta. A16AB05 Laronidase. A16AB06 Sacrosidase. A16AB07 Alglucosidase alfa. A16AB08 Galsulfase. A16AB09 ... A16AB16 Idursulfase beta. A16AB17 Cerliponase alfa. A16AB18 Vestronidase alfa. A16AX Various alimentary tract and metabolism ...
Different temperatures optimize the activity of alpha or beta amylase, resulting in different mixtures of fermentable and ...
... (EC 3.2.1.101, exo-1,6-beta-mannanase, endo-alpha-1-,6-D-mannanase, endo-1,6-beta-mannanase, ... mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan mannanohydrolase) is an enzyme with systematic name 6-alpha-D-mannan ...
... modeling beta-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta. 1770 (5): 733-46. doi:10.1016/j.bbagen. ...
Other genetically modified pigs have had alpha galactosidase transferase knocked out and fortified with hCD46 and the hTM ... "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): ... "Transgenic pigs designed to express human α-galactosidase to avoid humoral xenograft rejection". J Appl Genet. 54: 293-303. ...
... is used in a mixture with potassium ferricyanide and phosphate buffered solution to provide a buffer for beta-galactosidase, ... which is used to cleave X-Gal, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal ...
Alpha-galactosidase (EC 3.2.1.22) (melibiase) catalyzes the hydrolysis of melibiose into galactose and glucose. In man, the ... Raffinose (O-alpha- D-galactopyranosyl- (1-->6)- O-alpha- D-glucopyranosyl-(1<-->2)- O-beta- D-fructofuranoside) is a ... Escherichia coli alpha-galactosidase (gene melA), which requires NAD and magnesium as cofactors, is not structurally related to ... The sequence of this enzyme is highly related to that of the eukaryotic alpha-galactosidases. This family also includes ...
A list of US medications equivalent to Beta-Galactosidase (Penicillium) is available on the Drugs.com website. ... Beta-Galactosidase (Penicillium) is a medicine available in a number of countries worldwide. ... Ingredient matches for Beta-Galactosidase (Penicillium). Tilactase. Beta-Galactosidase (Penicillium) (JAN) is also known as ... Beta-Galactosidase (Penicillium). Beta-Galactosidase (Penicillium) may be available in the countries listed below. ...
A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... A new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) which is ... April 2006). "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111 ... β-galactosidase synthesis stops when glucose levels are sufficient. Beta-galactosidase has many homologues based on similar ...
Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.. J. Mol. Biol. 343 1281-92 2004 ... This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. It has a jelly roll fold [ ... Beta-galactosidase jelly roll domain (IPR025300). Short name: BetaGal_jelly_roll_dom ...
... Martin Kennedy mkennedy at chmeds.ac.nz Tue Oct 5 21:23:18 EST 1993 *Previous message: ... with betagalactosidase constructs? ,, I am interested in staining the cells alive by adding Xgal to the culture ,, media ... Previous message: beta galactosidase in cultured cells *Next message: beta galactosidase in cultured cells ...
... Justin Kemp justin.news.invalid at web2news.net Wed Feb 26 23:31:21 EST 2003 *Previous ... I have been told that beta-galactosidase may be suitable. Can anyone tell me if it is indeed devoid of carbohydrate? Any help ...
Posted in Medical HacksTagged adenovirus, beta-galactosidase, biohacking, enzyme, genetic engineering, lactase, lactose ... The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or ...
Endo-beta-galactosidase may refer to: Blood-group-substance endo-1,4-beta-galactosidase Keratan-sulfate endo-1,4-beta- ... galactosidase This set index page lists enzyme articles associated with the same name. If an internal link led you here, you ...
The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the ... Suicide gene therapy using E. coli beta-galactosidase.. Farquhar D1, Pan BF, Sakurai M, Ghosh A, Mullen CA, Nelson JA. ... Daun02 was a good substrate for beta-gal. By comparison, gal-DCN4 was a poor substrate. Except for PC3, the beta-gal-transduced ... O-beta- D-galactopyranosyl)butyl]daunorubicin (gal-DNC4) were investigated. The prodrugs were evaluated as substrates for beta- ...
BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542566.2 → XP_011540868.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542569.2 → XP_011540871.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542570.2 → XP_011540872.1 beta-galactosidase-1-like protein 3 ... GanA; Beta-galactosidase GanA [Carbohydrate transport and metabolism]. * XM_011542572.1 → XP_011540874.1 beta-galactosidase-1- ...
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
Beta-galactosidaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,O31341,O31341_BACCI Beta-galactosidase OS=Bacillus circulans GN=bgaC PE=1 SV=1 ...
Chicken polyclonal beta Galactosidase antibody. Validated in WB and tested in Escherichia coli. Immunogen corresponding to ... Anti-beta Galactosidase antibody (ab106567) at 1 µg/ml. Lane 1 : beta Galactosidase at 0.005 µg. Lane 2 : beta Galactosidase at ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... 17 amino acid peptide from near the N terminus of beta Galactosidase from E. coli (NP_752394). ...
β-galactosidase is found in plants, animals and microorganisms. In Beta-Galactosidase: Properties, Structure and Functions, the ... Beta-Galactosidase: Properties, Structure and Functions. Eloy Kras (Editor). Series: Cell Biology Research Progress. BISAC: ... Home / Shop / Books / Science and Technology / Life Sciences / Beta-Galactosidase: Properties, Structure and Functions. ... β-galactosidase is an enzyme responsible for catalyzing the hydrolysis of the lactose β-1,4 linkage into α-D-glucose and β-D- ...
Rabbit polyclonal GLB1/Beta-galactosidase antibody. Validated in IHC and tested in Mouse, Rat, Human. Cited in 5 publication(s ... Primary - Rabbit Anti-GLB1/Beta-galactosidase antibody (ab203749) IHC-P Protein - Recombinant Human GLB1/Beta-galactosidase ... Anti-GLB1/Beta-galactosidase antibody. See all GLB1/Beta-galactosidase primary antibodies. ... Isoform 2 has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic ...
Beta-galactosidaseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... sp,Q8X685,BGAL_ECO57 Beta-galactosidase OS=Escherichia coli O157:H7 OX=83334 GN=lacZ PE=3 SV=1 ... Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation. ,p>Manual validated ... IPR036156. Beta-gal/glucu_dom_sf. IPR011013. Gal_mutarotase_sf_dom. IPR008979. Galactose-bd-like_sf. IPR014718. GH-type_carb-bd ...
... and a complete beta-galactosidase reporter system. Fast, simple assay for beta-galactosidase activity. ... Colorimetric and chemiluminescent assays for beta galactosidase, LacZ vectors, ... Beta-Galactosidase Assay & LacZ Vectors. The beta-galactosidase assay is commonly used as a reporter, to monitor transfection ... Complete Beta-Gal Reporter System. The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta- ...
Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal ... Galactosidase Assay Reagent is a component of the Mammalian - ... Mammalian beta-Galactosidase Assay Kit. beta-Galactosidase ... The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which ... Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and- ...
Protein target information for Beta-galactosidase (Sinorhizobium meliloti). Find diseases associated with this biological ...
... "beta-Galactosidase" by people in Harvard Catalyst Profiles by year, and whether "beta-Galactosidase" was a major or minor topic ... non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1 ... "beta-Galactosidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "beta-Galactosidase" by people in Profiles. ...
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase ...
Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227 ... Caption: Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. ... Keywords: art, artwork, beta, biochemical, biochemistry, biological, biology, black background, compound, enzyme, galactosidase ...
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic ... Keywords: artwork, b-gal, beta galactosidase, beta-gal, beta-galactosidase, biochemical, biochemistry, cut out, cut outs, cut- ... Caption: Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their ...
Invitrogen Anti-beta Galactosidase Monoclonal (DC1 4C7), Catalog # MA1-152. Tested in Western Blot (WB), Immunofluorescence (IF ... Cite beta Galactosidase Monoclonal Antibody (DC1 4C7). The following antibody was used in this experiment: beta Galactosidase ... Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be ... coli beta Galactosidase at ~110 kDa. This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells. ...
The Beta-Galactosidase Staining Kit makes it easy to determine the transfection efficiency of mammalian cells using co- ... Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit.. One µg of a 119 kDa subunit of β-galactosidase was ... The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lacZ ... The gene product of lacZ, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily ...
High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques. 2004 Mar;36(3):410-5. PubMed ... Uses of lacZ to study gene function: evaluation of beta-galactosidase assays employed in the yeast two-hybrid system. Anal ... Ullmann A. Complementation in beta-galactosidase: from protein structure to genetic engineering. Bioessays. 1992 Mar;14(3):201- ... Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the ...
... beta-galactosidase explanation free. What is beta-galactosidase? Meaning of beta-galactosidase medical term. What does beta- ... Looking for online definition of beta-galactosidase in the Medical Dictionary? ... medical-dictionary.thefreedictionary.com/beta-galactosidase,beta-galactosidase,/a,. *Facebook ... A gene on chromosome 3p21.33 that encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyses the terminal beta-galactose ...
The isolated enzyme forms accounted for 83%, 8%, and 9% of the total beta-galactosidase activity, respectively … ... The enzyme beta-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using ... The beta-galactosidase forms were heat-stable up to about 60 degrees C. The Km values for lactose ranged from 85 mM to 125 mM, ... beta-Galactosidase From Aspergillus Niger. Separation and Characterization of Three Multiple Forms Eur J Biochem. 1979 Oct 15; ...
These protocols are used to screen bacterial plates for beta-galactosidase activity using X-Gal (aka blue white screening). ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Beta-galactosidase_Screen&oldid=537279" ...
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ...
  • Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. (abcam.com)
  • To test these hypotheses, we have inserted the rp51 intron into the coding region of a hybrid Escherichia coli beta-galactosidase gene, thereby interrupting the open reading frame subsequent to the initiating methionine codon. (pnas.org)
  • Beta-Galactosidase from Escherichia coli . (abnova.com)
  • Here we report the structure of a complex between Escherichia coli β-galactosidase and the cell-permeant inhibitor phenylethyl β-D-thiogalactopyranoside (PETG), determined by cryo-EM at an average resolution of ~2.2 angstroms (Å). (pdbj.org)
  • High purity β-galactosidase ( Escherichia coli ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Selectivity for lactose in the presence of structurally similar oligosaccharides was provided by using a β-galactosidase with much improved selectivity over the analytical industry standards from Aspergillus oryzae and Escherichia coli (EcLacZ), coupled with a 'creep' calculation adjustment to account for any overestimation. (megazyme.com)
  • 2. Scudder,P., Hanfland, Pl, Uemura, K. & Feizi, T. (1984) Endo-b-galactosidases of Bacteroides fragilis and Escherichia freundii hydrolyze linear but not branched oligosaccharide domains of glycolipids of the neolacto series. (qa-bio.com)
  • In this experiment, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme β-galactosidase. (studentshare.org)
  • Escherichia coli (E. coli) can produce the enzyme β-galactosidase which breaks lactose into galactose and glucose. (studentshare.org)
  • Altered expression of β-galactosidase-1-like protein 3 (Glb1l3) in the retinal pigment epithelium (RPE)-specific 65-kDa protein knock-out mouse model of Leber's congenital amaurosis. (nih.gov)
  • The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification. (thermofisher.com)
  • The kit can also be used to determine the efficiency of direct delivery of β-galactosidase by the Chariot™ Protein Delivery Reagent (Figure 1). (activemotif.com)
  • One µg of a 119 kDa subunit of β-galactosidase was complexed with Chariot™ Protein Delivery Reagent for 30 minutes and delivered into HeLa cells. (activemotif.com)
  • The V values obtained at 30 degrees C for lactose and o-nitrophenyl-beta-D-galactopyranoside were 104 units/mg enzyme protein and 121 units/mg enzyme protein, respectively (weighted averages for the three enzyme forms). (nih.gov)
  • WB analysis of beta Galactosidase protein (lanes1-3 for 60 ng, 30 ng and 15 ng, respectively) and non-specific control E.coli lysate (lane 4) using beta Galactosidase antibody. (acris-antibodies.com)
  • WB analysis of beta Galactosidase protein (1 μg) using beta Galactosidase antibody at a dilution of 1:1,000. (acris-antibodies.com)
  • WB analysis of non-reduced and reduced beta Galactosidase protein (lane1 for 80 ng and lane 2 for 20 ng) using beta Galactosidase antibody at a dilution of 1:10,000. (acris-antibodies.com)
  • Western blot experiment using Beta-gal Antibody (GTX124230) at 1:90000 dilution to detect 100ng of 55kDa recombinant Beta-gal protein antigen. (acris-antibodies.com)
  • Beta-galactosidase - protein Name = caption = β galactosidase from Penicillum sp. (academic.ru)
  • We conclude that the beta-galactosidase precursor is functionally identical to the mature lysosomal form of the enzyme and serves as an excellent enzyme source for investigation of structure-function relationships in the protein. (ubc.ca)
  • Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. (bio-rad-antibodies.com)
  • Chickens were immunized with purified ß-Galactosidase (lacZ) protein emulsified in Freund's adjuvants. (aveslabs.com)
  • Galactosialidosis is an autosomal recessive lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase secondary to a defect in the cathepsin A protein. (testcatalog.org)
  • Western blot analysis of Beta-gal expression in total protein extracts from transfected cells and transgenic mice expressing beta-gal protein. (euromabnet.com)
  • GLB1 (Galactosidase Beta 1) is a Protein Coding gene. (genecards.org)
  • Many CTSA mutations disrupt the protein structure of cathepsin A, impairing its ability to join with neuraminidase 1 and beta-galactosidase or elastin binding protein. (medlineplus.gov)
  • Most mutations in the CTSA gene cause a lack of functional cathepsin A and a loss of neuraminidase 1, beta-galactosidase, and elastin binding protein. (medlineplus.gov)
  • New mutations in the PPBG gene lead to loss of PPCA protein which affects the level of the beta-galactosidase/neuraminidase complex and the EBP-receptor. (medlineplus.gov)
  • β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose. (wikipedia.org)
  • Beta-galactose is used in such dairy products as yogurt, sour cream, and some cheeses which are treated with the enzyme to break down any lactose before human consumption. (wikipedia.org)
  • In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikipedia.org)
  • In E. coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. (thermofisher.com)
  • The beta-galactosidase forms were heat-stable up to about 60 degrees C. The Km values for lactose ranged from 85 mM to 125 mM, whereas those for the synthetic substrate o-nitrophenyl-beta-D-galactopyranoside were equal to about 2.4 mM. (nih.gov)
  • Le β galactoside plus commun et connu en biochimie est le lactose. (academic.ru)
  • This book describes the reaction rate profiles of the beta-galactosidase-catalyzed conversion of lactose on the inner surface of a hollow fiber membrane, which is employed as an enzymatic reactor system. (weltbild.ch)
  • In this study, a deoxyribozyme against α-peptide beta-galactosidase gene in the lactose operon was designed. (magiran.com)
  • A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. (ufrgs.br)
  • The lactosucrose was produced by transgalactosylation that was catalyzed by a Bacillus circulans β-galactosidase immobilized on macrospheres of chitosan using lactose and sucrose as substrates. (ufrgs.br)
  • Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides. (bio-rad-antibodies.com)
  • The results support lactose metabolism by newly synthesised beta-galactosidase and also, quantitatively, IPTG is a more effective inducer of beta-galactosidase synthesis than lactose. (studentshare.org)
  • Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose, the enzymes natural substrate (Ring, 1999, 80). (studentshare.org)
  • Beta-galactosidase (B-gal for short) is an enzyme that will process the substrate lactose. (stackexchange.com)
  • The beta-galactosidase (EC 3.2.1.32) of Corynebacterium murisepticum (inducible by lactose and galactose) was purified by successive column chromatography on Sephadex G-200, DEAE-Sephadex A-50 and DEAE-cellulose (DE52). (meta.org)
  • The Km values of the enzyme for the substrates lactose and o-nitrophenyl-beta-D-galactopyranoside (ONPG) are 16.7 mM and 4.4 mM, respectively, indicating, its low affinity for the substrates. (meta.org)
  • The EC (Enzyme Commission) number of β-galactosidase is 3.2.1.23 β-galactosidase belongs to class 3, which refers to the hydrolases. (wikipedia.org)
  • The enzyme beta-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using molecular sieving, ion-exchange, an hydrophobic chromatography. (nih.gov)
  • Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) Beta Galactosidase (Beta-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the Beta-galactosides into monosacchanides. (assaygenie.com)
  • Beta Galactosidase (Beta-Gal) Inhibitor Screening Kit (Fluorometric) Beta Galactosidase (Beta-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the. (assaygenie.com)
  • The food enzyme β‐d‐galactosidase galactohydrolase (EC 3.2.1.23) is produced with Bacillus sp. (europa.eu)
  • Suicide gene therapy using E. coli beta-galactosidase. (nih.gov)
  • The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the prodrug-activating enzyme. (nih.gov)
  • Western blot analysis of MA1-152 detects recombinant E. coli beta Galactosidase at ~110 kDa. (thermofisher.com)
  • This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells. (thermofisher.com)
  • E. coli beta Galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression. (thermofisher.com)
  • This Fab fragment recognizes wild-type E. coli beta-galactosidase. (abnova.com)
  • We offer two ways to detect LacZ gene activity: a colorimetric beta-galactosidase staining kit and a chemiluminescent beta-galactosidase assay. (clontech.com)
  • The Beta-Galactosidase Staining Kit is a complete system for colorimetric detection of beta-galactosidase expression in mammalian cells. (clontech.com)
  • The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lac Z following transient or stable transfection of plasmids that contain lac Z. (activemotif.com)
  • Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit. (activemotif.com)
  • Two hours post-delivery, cells were fixed and stained using the β-Galactosidase Staining Kit. (activemotif.com)
  • The Beta-Galactosidase Staining Kit is a complete system for X-gal detection of beta-galactosidase expression in mammalian cells. (takarabio.com)
  • The Beta-Galactosidase staining kit utilizes X-gal as the substrate. (assaygenie.com)
  • The Beta-Galactosidase staining kit utilizes X-gal as the substrate and LacZ gene as the reporter for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. (assaygenie.com)
  • β-galactosidase, also called lactase, beta-gal or β-gal, is a family of glycoside hydrolase enzymes that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. (wikipedia.org)
  • A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase-phlorizin hydrolase. (wikipedia.org)
  • Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (wikipedia.org)
  • The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or lactase, and use it to infect the cells of his small intestine. (hackaday.com)
  • Synthetic peptide within Human GLB1/Beta-galactosidase aa 315-365 conjugated to keyhole limpet haemocyanin. (abcam.com)
  • The disorder is "caused by mutations in the GLB1 gene, which encodes an enzyme called beta-galactosidase necessary for recycling of a molecule (GM1-ganglioside) in neurons. (thefreedictionary.com)
  • β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. (wikipedia.org)
  • Beta-gal is inhibited by L-ribose, non-competitive inhibitor iodine, and competitive inhibitors 2-phenylethyl 1-thio-beta-D-galactopyranoside (PETG), D-galactonolactone, isopropyl thio-beta-D-galactoside (IPTG), and galactose. (wikipedia.org)
  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. (rcsb.org)
  • A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. (harvard.edu)
  • Beta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety. (thermofisher.com)
  • A gene on chromosome 3p21.33 that encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyses the terminal beta-galactose from gangliosides and other glycoconjugates. (thefreedictionary.com)
  • It is an autosomal recessive sphingolipidosis caused by deficient activity of the lysosomal enzyme galactosylceramide beta-galactosidase (GALC) which degrades galactosylceramide, a main component of myelin, and other terminal beta-galactose-containing sphingolipids, including psychosine (galactosylsphingosine). (thefreedictionary.com)
  • This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates. (acris-antibodies.com)
  • Beta-galactoside - A β galactoside is a type of galactoside in which the glycosidic bond lies above the plane of the galactose residue. (academic.ru)
  • beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. (uzh.ch)
  • This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. (genecards.org)
  • Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. (thermofisher.com)
  • Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. (abnova.com)
  • The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikipedia.org)
  • In this kit, beta-galactosidase (the product of the LacZ gene) catalyzes the hydrolysis of X-gal to produce a blue color that is easily visualized. (clontech.com)
  • The gene product of lac Z, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily visualized. (activemotif.com)
  • Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Gal present in a partially purified preparation (arrowhead). (acris-antibodies.com)
  • This antibody is useful for the detection of Beta-Galactosidase in ELISA, Western Blot and other immuno-based applications. (afsbio.com)
  • Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. (uzh.ch)
  • We show that the stereochemical course of enzymic hydrolysis involves the retention of the beta-configuration at the anomeric centre, suggesting a double-displacement mechanism. (ubc.ca)
  • Beta-galactosidase is a lysosomal enzyme responsible for catalyzing the hydrolysis of gangliosides. (testcatalog.org)
  • Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. (acris-antibodies.com)
  • Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research. (acris-antibodies.com)
  • Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). (acris-antibodies.com)
  • Beta-galactosidase, molecular model. (sciencephoto.com)
  • beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. (bio-rad-antibodies.com)
  • Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. (harvard.edu)
  • ClinicalTrials.gov lists trials that are related to Beta-galactosidase-1 deficiency. (nih.gov)
  • The prodrugs were evaluated as substrates for beta-gal. (nih.gov)
  • The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others. (thermofisher.com)
  • The LacZ gene encodes the enzyme Beta-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ. (assaygenie.com)
  • The Galacto-Light™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays. (selectscience.net)
  • The kit contains sufficient reagents to perform 120 beta-galactosidase staining assays in 35 mm dishes. (takarabio.com)
  • Beta-Galactosidase (Penicillium) may be available in the countries listed below. (drugs.com)
  • Crystal structures of beta-galactosidase from Penicillium sp. (ebi.ac.uk)
  • Beta-galactosidase is the product of the LacZ gene. (clontech.com)
  • We offer three beta-gal mammalian reporter vectors containing the LacZ gene. (clontech.com)
  • A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase. (openwetware.org)
  • In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity. (semanticscholar.org)
  • Our Beta-Galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. (aveslabs.com)
  • pCMV-LacZ is a constitutive mammalian reporter vector which expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter ( P CMV IE ) and can be used as a reference or control plasmid. (clontech.com)
  • Then the levels of beta-galactosidase were compared. (stackexchange.com)
  • EMDB-2984: 2.2 A resolution cryo-EM structure of beta-galactosidase in compl. (pdbj.org)
  • EMDB-8908: Cryo-EM structure of beta-galactosidase using RELION on Amazon We. (pdbj.org)
  • The P10 promoter directed the synthesis of beta-galactosidase, whereas the polyhedrin promoter controlled the synthesis of foreign gene products. (asm.org)
  • Download file to see previous pages Apart from induction, synthesis rate is determined by catabolite repression, whereby it slows down the synthesis of beta-galactosidase especially in the presence of a better carbon (and energy) source, such as glucose. (studentshare.org)
  • The use Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml is much more reproducable than with a polyclonal antibody. (antibody-antibodies.com)
  • Daun02 was a good substrate for beta-gal. (nih.gov)
  • see below) and Detection Kit II (reagents only) provide quantitative measurement of beta-gal activity using a chemiluminescent substrate. (clontech.com)
  • beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. (ox.ac.uk)
  • this strain was identified with an enzyme substrate test which demonstrated that the organism produced beta-galactosidase. (cdc.gov)
  • 66586-1-Ig targets Beta galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with Human samples. (ptglab.com)
  • The following antibody was used in this experiment: beta Galactosidase Monoclonal Antibody (DC1 4C7) from Thermo Fisher Scientific, catalog # MA1-152, RRID AB_2536860. (thermofisher.com)
  • Mouse monoclonal antibody raised against Beta-Galactosidase. (abnova.com)
  • The beta Galactosidase Antibody (DC3 10D6) [DyLight 755] from Novus Biologicals is a mouse monoclonal antibody to beta Galactosidase. (vwr.com)
  • Validation of 3A9A monoclonal antibody in Hek-V5-Beta-gal transfected cells. (euromabnet.com)
  • Rat monoclonal 3A9A can be used to detect Beta-Gal over expression on mouse paraffin sections. (euromabnet.com)
  • Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml monclonal andibody monoclonal antobodies are directed against a specific epitope. (antibody-antibodies.com)
  • Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml immunoglobuline1 The Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml is very stable with a high affinity. (antibody-antibodies.com)
  • These two genes recombined with wild-type virus genome to yield recombinants which were polyhedrin negative, produced the foreign gene product, and formed blue plaques when beta-galactosidase indicator was present in the agarose overlay. (asm.org)
  • Portanto, o bioprocesso de imobilização de β-galactosidase de Bacillus circulans em macroesfera de quitosana pode ser considerado um potencial catalisador para produção industrial de lactosacarose. (ufrgs.br)
  • Therefore, the bioprocess of β-galactosidase from Bacillus circulans immobilization on macrospheres of chitosan can be considered a potential catalyst for industrial. (ufrgs.br)
  • Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (europa.eu)
  • The beta Galactosidase Antibody (DC3 10D6) [DyLight 755] has been validated for the following applications: ELISA. (vwr.com)
  • Advantages of this approach include: (1) the ability to use prodrugs that are cleaved by beta-gal to agents that are known to possess activity against human solid tumors, and (2) the extensive experience gained with targeting beta-gal to specific tumors in experimental animals and in humans. (nih.gov)
  • Beta-galactosidase activity can be detected with the Luminescent Beta-Gal Detection Kit II. (clontech.com)
  • The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity. (thermofisher.com)
  • The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. (thermofisher.com)
  • Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. (thermofisher.com)
  • Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells. (thermofisher.com)
  • Of note, A[sz] did not induce the senescence-associated beta-galactosidase activity in cultured neurons (data not shown), indicating that SA-[sz]-gal was not required for neuronal senescence in vitro , although it was a classic CS biomarker. (thefreedictionary.com)
  • Toussaint, "Protocols to detect senescence-associated beta-galactosidase (SA-[beta]gal) activity, a biomarker of senescent cells in culture and in vivo," Nature Protocols, vol. (thefreedictionary.com)
  • Bone marrow transplantation correcting beta-galactosidase activity does not influence neurological outcome in juvenile [G. (thefreedictionary.com)
  • The isolated enzyme forms accounted for 83%, 8%, and 9% of the total beta-galactosidase activity, respectively. (nih.gov)
  • These protocols are used to screen bacterial plates for beta-galactosidase activity using X-Gal (aka blue white screening). (openwetware.org)
  • The presence of the rp51 intron causes the beta-galactosidase activity to be sensitive to the rna2 mutation, consistent with the notion that this mutation affects gene expression at the level of splicing. (pnas.org)
  • X-Gal staining for beta-galactosidase activity was used to study gene transfer efficiency and distribution of the marker gene. (nih.gov)
  • One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p -nitrophenol per minute from p -nitrophenyl-β-D-galactoside (10 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40 o C. (megazyme.com)
  • The precursor is readily taken up in a mannose-6-phosphate-dependent manner into beta-galactosidase-deficient, GM1-gangliosidosis fibroblasts, and the enzyme activity is returned to normal levels. (ubc.ca)
  • 4. Murata, T., Hattori, T. Amarume, S. Koicki, A. & Usui, T. (2003) Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and sythesis of N-acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity. (qa-bio.com)
  • Specific Activity One unit of Beta-(1-3,4,6)-Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 4.0 from p-nitrophenyl-Beta-D-galactopyranoside. (qa-bio.com)
  • The Luminescent β-galactosidase Detection Kit II is a complete system for the chemiluminescent detection of β-galactosidase enzyme activity. (takarabio.com)
  • Senescence Detection Kit (SA-beta-gal Staining) Senescence Detection Kit is a Fast, Convenient & Easy-to-use kit that measures activity of SA-B-Gal in cells. (assaygenie.com)
  • and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. (bartleby.com)
  • GM1 gangliosidosis is an autosomal recessive lysosomal storage disorder caused by reduced or absent beta-galactosidase activity. (testcatalog.org)
  • MPS IVB is caused by a reduced or absent activity of the beta-galactosidase enzyme and gives rise to the physical manifestations of the disease. (testcatalog.org)
  • Isoform 2: Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. (genecards.org)
  • Is there any alternative to x-gal white/blue screening for the monitoring of pseudomonas aeruginosa beta-galactosidase activity on agar plate? (stackexchange.com)
  • Gene Ontology (GO) annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and beta-galactosidase activity . (genecards.org)
  • The renal glomerular and tubule impairment was evaluated by determination of cystatin C (Cys-C) in the serum and microalbumin (mALB), urinary beta(2)-microglobulin ( (2)M), N-acetyl-beta-D-glucosaminidase (NAG) activity in the urine. (ilo.org)
  • This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. (ebi.ac.uk)
  • Saccharomyces fragilis is described as a homothallic, hemiascomycetous yeast and production of several enzymes among them beta-galactosidase (Llorente et al. (thefreedictionary.com)
  • The biological significance of A. niger beta-galactosidase multiplicity might be related to the observed differences in carbohydrate content, as suggested by recent reports on other microbial glycoprotein enzymes. (nih.gov)
  • Cathepsin A interacts with the enzymes β-galactosidase and neuraminidase 1, which play a role in the breakdown of complexes of sugar molecules (oligosaccharides) attached to certain proteins (glycoproteins) or fats (glycolipids). (medlineplus.gov)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9). (ptglab.com)
  • Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. (bio-rad-antibodies.com)
  • Expression of Beta-gal (3A9A) antigen by mouse pancreatic cells. (euromabnet.com)
  • FDG Beta-Galactosidase Assay Kit FDG Beta-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active. (assaygenie.com)
  • ONPG Galactosidase Assay Kit ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active. (assaygenie.com)
  • Computer model showing the 4-chain-structure of bacterial beta-galactosidase. (sciencephoto.com)
  • Bacterial beta -galactosidase as a marker of Rous sarcoma virus gene expression and replication. (sciencebase.gov)
  • Beta-galactosidase enzyme is deficient in the following conditions: GM1 gangliosidosis, Morquio syndrome B, and galactosialidosis. (mayocliniclabs.com)
  • Alpha-galactosidase - is a glycoside hydrolase enzyme that hydrolyses the terminal alpha galactosyl moieties from glycolipids and glycoproteins. (academic.ru)
  • Glycoside hydrolase, family 2 / Beta galactosidase small chain/ domain 5 / Domain of unknown function(DUF4981) / Glycosyl hydrolases family 2 acid/base catalyst. (pdbj.org)
  • Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. (uzh.ch)
  • The protocol describes chemiluminescent detection of β-galactosidase via a scintillation counter, tube or plate luminometer, or x-ray film. (takarabio.com)
  • Isoform 1: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. (genecards.org)
  • Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. (asm.org)
  • A series of plasmids encoding hybrid proteins comprising various lengths of the NH2-terminal region of colicin N coupled to almost complete beta-galactosidase or alkaline phosphatase polypeptides was constructed by transposon mutagenesis of ColN plasmid derivatives. (epfl.ch)
  • Large quantities of the hybrid proteins accumulated in the cytoplasm (beta-galactosidase) or particulate fractions (alkaline phosphatase). (epfl.ch)
  • Reference : Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Deter. (ac.be)
  • beta-Galactosidase From Aspergillus Niger. (nih.gov)
  • Li X, Jiang Y, Chong S, Walt DR. Bottom-up single-molecule strategy for understanding subunit function of tetrameric ß-galactosidase. (harvard.edu)
  • 1. A process for producing a galactosyl.beta.1,3glycal disaccharide that comprises admixing (i) a galactoside donor molecule, (ii) glucal, 6-O--C.sub.1 -C.sub.6 acyl glucal or6-O--C.sub.1 -C.sub.6 acyl galactal as acceptor and (iii) a catalytic amount of .beta. (patentgenius.com)
  • Chinese hamster ovary cell clones permanently transfected with the cDNA for human lysosomal beta-galactosidase secrete the enzyme precursor into the cell medium, from which it is purified to apparent homogeneity in a single step by affinity chromatography. (ubc.ca)
  • IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. (acris-antibodies.com)
  • The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta-galactosidase assay reagents plus the pBeta-Gal-Basic and pBeta-Gal-Control vectors. (clontech.com)
  • Beta-galactosidase gene transfer was well tolerated with both vectors. (nih.gov)
  • beta-galactosidase rat anti E.coli, clone 3A9A. (euromabnet.com)
  • Anti-beta-gal (Clone 3A9A) immunohistochemistry in mouse paraffin tissues. (euromabnet.com)
  • Despite the presence of the intron, the beta-galactosidase gene is expressed in yeast. (pnas.org)
  • In the University labs, we have used Beta-galactosidase as a reporter gene to quantify the expression initiated by the stress-response promoter in yeast. (stackexchange.com)
  • The 1,023 amino acids of E. coli β-galactosidase were accurately sequenced in 1983, and its structure determined twenty-four years later in 1994. (wikipedia.org)
  • A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase. (openwetware.org)
  • The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies. (clontech.com)
  • What are the advantages and disadvantages of using beta-galactosidase compared to luciferase as a reporter gene? (stackexchange.com)
  • A question given to us afterwards asked to give advantages & disadvantages of using Beta-galactosidase compared to Luciferase as a reporter gene in this experiment. (stackexchange.com)