OrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsInformation Science
beta-GalactosidaseGalactosidasesalpha-GalactosidaseComplement C3cCerebrosidesLactoseGalactoseAcridine Orangealpha-L-FucosidaseFabry DiseaseNigericinGlycoside HydrolasesGlycosidesCell AgingHistocytochemistryInterleukin-1betaEscherichia colibeta 2-MicroglobulinMolecular Sequence DataReceptors, Adrenergic, betaIntegrin beta3Pan troglodytesApe DiseasesPan paniscusGorilla gorillaTool Use BehaviorBase SequenceGestures
beta-Galactosidase
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Galactosidases
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
alpha-Galactosidase
An enzyme that catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-galactosides including galactose oligosaccharides, galactomannans, and galactolipids.
Complement C3c
A 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by COMPLEMENT FACTOR I into C3c (749-954), and C3dg (955-1303) in the presence COMPLEMENT FACTOR H.
Cerebrosides
Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)
Lactose
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
Galactose
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Acridine Orange
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
alpha-L-Fucosidase
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
Fabry Disease
An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.
Nigericin
A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)
Glycoside Hydrolases
Glycosides
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Cell Aging
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Histocytochemistry
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Interleukin-1beta
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
beta 2-Microglobulin
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Receptors, Adrenergic, beta
One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.
Integrin beta3
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
Pan troglodytes
The common chimpanzee, a species of the genus Pan, family HOMINIDAE. It lives in Africa, primarily in the tropical rainforests. There are a number of recognized subspecies.
Ape Diseases
Diseases of chimpanzees, gorillas, and orangutans.
Pan paniscus
The pygmy chimpanzee, a species of the genus Pan, family HOMINIDAE. Its common name is Bonobo, which was once considered a separate genus by some; others considered it a subspecies of PAN TROGLODYTES. Its range is confined to the forests of the central Zaire basin. Despite its name, it is often of equal size to P. troglodytes.
Gorilla gorilla
This single species of Gorilla, which is a member of the HOMINIDAE family, is the largest and most powerful of the PRIMATES. It is distributed in isolated scattered populations throughout forests of equatorial Africa.
Tool Use Behavior
Modifying, carrying, or manipulating an item external to itself by an animal, before using it to effect a change on the environment or itself (from Beck, Animal Tool Behavior, 1980).
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Gestures
Movement of a part of the body for the purpose of communication.

Fecal coliform elevated-temperature test: a physiological basis. (1/7076)

The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.5 degrees C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5 degrees C. A temperature increase from 35 to 44.5 degrees C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform beta-galactosidase activity coupled with thermal inactivation of this enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.  (+info)

The effects of digestive enzymes on characteristics of placental insulin receptor. Comparison of particulate and soluble receptor preparations. (2/7076)

The role of the surrounding membrane structure on the binding characteristics of the insulin receptor was studied by using several digestive enzymes. The effects observed with particulate membrane preparations are compared with those from soluble receptor preparations. beta-Galactosidase and neuraminidase had no effect on insulin binding to either particulate or soluble receptors from human placentae. Exposure to 2 units of phospholipase C/ml increased insulin binding to particulate membranes, but was without effect on the soluble receptor preparation. The increase in binding to particulate membranes was shown to be due to an increase in apparent receptor number. After 5 min exposure to 500 microgram of trypsin/ml there was an increase in insulin binding to the particulate membrane fraction, owing to an increase in receptor affinity. After 15 min exposure to this amount of trypsin, binding decreased, owing to a progressive decrease in receptor availability. In contrast, this concentration of trypsin had no effect on the solubilized receptor preparation. Because of the differential effects of phospholipase C and trypsin on the particulate compared with the solubilized receptor preparations, it is concluded that the effects of these enzymes were due to an effect on the surrounding membrane structure. Changes in receptor configuration due to alterations within the adjoining membrane provide a potential mechanism for mediating short-term alterations in receptor function.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (3/7076)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (4/7076)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Pathogenicity island 2 mutants of Salmonella typhimurium are efficient carriers for heterologous antigens and enable modulation of immune responses. (5/7076)

The potential use as vaccine delivery system of Salmonella typhimurium strains harboring defined mutations in the sseC (HH104) and sseD (MvP101) genes, which encode putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2, was evaluated and compared with that of the well-characterized aroA mutant strain SL7207 by using beta-galactosidase (beta-Gal) as a model antigen. When orally administered to immune-competent or gamma interferon-deficient (IFN-gamma-/-) BALB/c mice, both mutants were found to be highly attenuated (50% lethal dose, >10(9) bacteria). Both strains were also able to efficiently colonize and persist in Peyer's patches. Immunization with HH104 and MvP101 triggered beta-Gal-specific serum and mucosal antibody responses equivalent to or stronger than those observed in SL7207-immunized mice. Although immunoglobulin G2 (IgG2) serum antibodies were dominant in all groups, IgG1 was also significantly increased in mice vaccinated with MvP101 and SL7207. Comparable beta-Gal-specific IgA and IgG antibodies were detected in intestinal lavages from mice immunized with the different strains. Antigen-specific CD4(+) T-helper cells were generated after vaccination with all vaccine prototypes; however, responses were significantly more efficient when HH104 and MvP101 were used (P < 0.05). Significantly higher levels of IFN-gamma were produced by restimulated spleen cells from mice immunized with HH104 than from those vaccinated with the MvP101 or SL7207 derivatives (P +info)

Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes. (6/7076)

BACKGROUND: In congestive heart failure, high systemic levels of the hormone arginine vasopressin (AVP) result in vasoconstriction and reduced cardiac contractility. These effects are mediated by the V1 vasopressin receptor (V1R) coupled to phospholipase C beta-isoforms. The V2 vasopressin receptor (V2R), which promotes activation of the Gs/adenylyl cyclase system, is physiologically expressed in the kidney but not in the myocardium. Expression of a recombinant V2R (rV2R) in the myocardium could result in a positive inotropic effect via the endogenous high concentrations of AVP in heart failure. METHODS AND RESULTS: A recombinant adenovirus encoding the human V2R (Ad-V2R) was tested for its ability to modulate the cardiac Gs/adenylyl cyclase system and to potentiate contractile force in rat ventricular cardiomyocytes and in H9c2 cardiomyoblasts. Ad-V2R infection resulted in a virus concentration-dependent expression of the transgene and led to a marked increase in cAMP formation in rV2R-expressing cardiomyocytes after exposure to AVP. Single-cell shortening measurements showed a significant agonist-induced contraction amplitude enhancement, which was blocked by the V2R antagonist, SR 121463A. Pretreatment of Ad-V2R-infected cardiomyocytes with AVP led to desensitization of the rV2R after short-term agonist exposure but did not lead to further loss of receptor function or density after long-term agonist incubation, thus demonstrating resistance of the rV2R to downregulation. CONCLUSIONS: Adenoviral gene transfer of the V2R in cardiomyocytes can modulate the endogenous adenylyl cyclase-signal transduction cascade and can potentiate contraction amplitude in cardiomyocytes. Heterologous expression of cAMP-forming receptors in the myocardium could lead to novel strategies in congestive heart failure by bypassing the desensitized beta-adrenergic receptor signaling.  (+info)

Multiple cis-acting regulatory regions are required for restricted spatio-temporal Hoxa5 gene expression. (7/7076)

Genetic analyses have revealed the essential role of the murine Hoxa5 gene for the correct specification of the cervical and upper thoracic region of the skeleton, and for the normal organogenesis and function of the respiratory tract, both structures expressing Hoxa5 during embryogenesis. To understand how the expression domains of the Hoxa5 gene are established during development, we have analyzed the cis-acting control regions mediating Hoxa5 gene expression using a transgenic approach. Four transcripts are derived from the Hoxa5 locus. The shortest and most abundant one displays a specific spatio-temporal profile of expression at earlier stages and in more anterior structures along the embryonic axis than the larger forms. We established that an 11.1 kilobase pair (kb) genomic fragment, extending from position -3.8 kb to +7.3 kb relative to Hoxa5 transcription initiation site, was sufficient to reproduce the temporal expression and substantially reconstitute the spatial pattern of the major Hoxa5 transcript. By deletion analyses, we identified a 2.1 kb fragment located downstream of the Hoxa5 gene that possesses mesodermal enhancer activity. Overall, the findings demonstrate that cis-acting regulatory elements essential for the correct expression of the major Hoxa5 transcript are located both upstream and downstream of the Hoxa5 coding sequences.  (+info)

Ectopic expression of the transforming growth factor beta type II receptor disrupts mesoderm organisation during mouse gastrulation. (8/7076)

Transforming growth factor beta (TGFbeta) regulates the cell cycle and extracellular matrix (ECM) deposition of many cells in vitro. We have analysed chimaeric mouse embryos generated from embryonic stem cells with abnormal receptor expression to study the effect of TGFbeta on these processes in vivo and the consequences for normal development. The binding receptor for TGFbeta, TbetaRII, is first detected in the embryo proper around day 8.5 in the heart. Ectopic expression of TbetaRII from the blastocyst stage onward resulted in an embryonic lethal around 9.5 dpc. Analysis of earlier stages revealed that the primitive streak of TbetaRII chimaeras failed to elongate. Furthermore, although cells passed through the streak and initially formed mesoderm, they tended to accumulate within the streak. These defects temporally and spatially paralleled the expression of the TGFbeta type I receptor, which is first expressed in the node and primitive streak. We present evidence that classical TGFbeta-induced growth inhibition was probably the cause of insufficient mesoderm being available for paraxial and axial structures. The results demonstrate that (1) TGFbeta mRNA and protein detected previously in early postimplantation embryos is present as a biologically active ligand; and (2) assuming that ectopic expression of TbetaRII results in no other changes in ES cells, the absence of TbetaRII is the principle reason why the embryo proper is unresponsive to TGFbeta ligand until after gastrulation.  (+info)

Beta Galactosidase | Infospaze - Sharing Wisdom by Community.
Beta Galactosidase | Learn more about Beta Galactosidase | Meaning of Beta Galactosidase | Description of Beta Galactosidase | Details of Beta Galactosidase | Article on Beta Galactosidase | Essay on Beta Galactosidase | Definition of Beta Galactosidase | Infospaze
http://www.infospaze.com/Beta_galactosidase
Flashcards - microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSIFlashcards - microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
https://www.freezingblue.com/flashcards/print_preview.cgi?cardsetID=244774
Quantitation of β-galactosidase activity. In yeast cel | Open-iQuantitation of β-galactosidase activity. In yeast cel | Open-i
Quantitation of β-galactosidase activity. In yeast cells, co-transformed with pGADT7 (AD) and pGBKT7 (BD) constructs as indicated, β-galactosidase activity wa
https://openi.nlm.nih.gov/detailedresult.php?img=PMC1173108_1471-2180-5-27-6&req=4
Beta-Galactosidase Assay, β-Galactosidase StainingBeta-Galactosidase Assay, β-Galactosidase Staining
Beta-Galactosidase Assay, β-Galactosidase StainingBeta-Galactosidase Assay, β-Galactosidase Staining
Recombinant E. coli beta Galactosidase protein (ab79449)Recombinant E. coli beta Galactosidase protein (ab79449)
Buy our Recombinant |em|E. coli |/em| beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols…
http://www.abcam.com/recombinant-e-coli-beta-galactosidase-protein-ab79449.html
Whole‐Mount Histochemical Detection of β‐Galactosidase Activity - Current ProtocolsWhole‐Mount Histochemical Detection of β‐Galactosidase Activity - Current Protocols
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
http://www.currentprotocols.com/WileyCDA/CPUnit/refId-mb1414.html
Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software ...Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software ...
BioTek Notes dapplication, 26-Feb-07, Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software
https://www.biotek.fr/fr/resources/application-notes/kinetic-analysis-of-galactosidase-activity-using-powerwave-ht-microplate-spectrophotometer-and-gen5-data-analysis-software/
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine...Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine...
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
http://orbi.ulg.ac.be/handle/2268/33007
Anti Beta Galactosidase Antibody | Bio-RadAnti Beta Galactosidase Antibody | Bio-Rad
|strong|Rabbit anti beta-galactosidase polyclonal antibody|/strong| detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccha…
https://www.bio-rad-antibodies.com/polyclonal/bacterial-beta-galactosidase-antibody-ahp1292.html
Optimisation of an enzymatic method for beta-galactosidase. - Nuffield Department of Surgical Sciences
BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12
https://www.nds.ox.ac.uk/publications/142130
Beta-galactosidase molecule - Stock Image C035/6227 - Science Photo LibraryBeta-galactosidase molecule - Stock Image C035/6227 - Science Photo Library
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
http://www.sciencephoto.com/media/847800/view
Antibody conjugate-Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate-Genemed Synthesis Inc.
Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN
http://genemedsyn.com/ccpc1034-5204-anti-beta-galactosidase-28e--coli29-igg-biotin-conj-antibody-conjugate-bgal12-btn.htm
Genetic Analysis of nif Regulatory Genes by Utilizing the Yeast Two-Hybrid System Detected Formation of a NifL-NifA Complex...Genetic Analysis of nif Regulatory Genes by Utilizing the Yeast Two-Hybrid System Detected Formation of a NifL-NifA Complex...
a The β-galactosidase activity assay was performed as described in the MATCHMAKER two-hybrid system (20). Briefly, cells from 1.5-ml samples of exponential culture were collected by centrifugation and resuspended in 300 μl of Z-buffer (20). A 100-μl aliquot of the resuspended cells was lysed by quick freeze-thaw (treatment with liquid nitrogen followed by thawing at 37°C). To measure the β-galactosidase activity in the cell lysate, a 0.7-ml sample of the Z-buffer-β-mercaptoethanol solution was added to each tube followed by 0.16 ml of Z-buffer-ONPG (4 mg of ONPG per 1 ml of Z-buffer). The time of ONPG addition was recorded, and the tubes were incubated at 30°C with shaking. When yellow color was visible, 400 μl of 1 M NaCO3 was added to each tube to terminate the reaction, and the time was recorded. The tubes were then centrifuged for 10 min at 10,000 × g to remove cellular debris, and the optical density at 420 nm was recorded. β-Galactosidase units were defined as the amount of ...
https://jb.asm.org/content/181/20/6535/figures-only
Colony Lift Colorimetric Assay for -Galactosidase Activity
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
http://molecularcloning.com/index.php?prt=246
RCSB PDB - 1DP0: E. COLI BETA-GALACTOSIDASE AT 1.7 ANGSTROM Methods Report PageRCSB PDB - 1DP0: E. COLI BETA-GALACTOSIDASE AT 1.7 ANGSTROM Methods Report Page
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
http://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=1DP0
Beta-galactosidase - OpenWetWareBeta-galactosidase - OpenWetWare
Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.) ...
https://openwetware.org/wiki/?title=Beta-galactosidase&oldid=194455
Efficient repopulation of denuded rabbit arteries with autologous genetically modified endothelial cells. | CirculationEfficient repopulation of denuded rabbit arteries with autologous genetically modified endothelial cells. | Circulation
METHODS AND RESULTS The method involves the harvest of autologous venous-derived endothelial cells, the efficient genetic modification of the cells through the use of recombinant retroviruses, and the subsequent implantation of the genetically modified cells on the surface of balloon-denuded arterial segments. With a rabbit model, freshly isolated endothelial cells were transduced with a recombinant retrovirus encoding the bacterial enzyme beta-galactosidase. The autologous transduced cells were then implanted on the surface of balloon-denuded ileofemoral arterial segments at different cell densities; after 1 to 14 days, the animals were killed, and the vessel segments were examined. Cells expressing the bacterial gene product, as determined by in situ staining for beta-galactosidase, were found to be present on the surface of 28 of the 32 arteries seeded with genetically modified cells. Vessels examined at 4 to 7 days after seeding displayed 40% to 90% coverage with transduced cells, even when ...
http://circ.ahajournals.org/content/89/5/2161
Beta-galactosidase - TA505568 | acris-antibodies.com
Beta-galactosidase, 0.1 ml. This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates.
https://www.acris-antibodies.com/antibodies/primary-antibodies/beta-galactosidase-ta505568.htm
Beta-galactosidase molecule - Stock Image C015/1970 - Science Photo LibraryBeta-galactosidase molecule - Stock Image C015/1970 - Science Photo Library
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic units (monosaccharides). - Stock Image C015/1970
http://www.sciencephoto.com/media/497185/view
Alfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade | Fisher ScientificAlfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade | Fisher Scientific
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade Quantity:
https://www.fishersci.com/shop/products/beta-galactosidase-digest-standard-certified-mass-spec-grade-2/aaj67822odl
RCSB PDB for 1DP0RCSB PDB for 1DP0
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
http://www.rcsb.org/pdb/explore/literature.do?structureId=1DP0&bionumber=1
The cae8 promoter is sensitive to the amino acid L-aspa | Open-iThe cae8 promoter is sensitive to the amino acid L-aspa | Open-i
The cae8 promoter is sensitive to the amino acid L-asparagine.The figure shows measured β-galactosidase activities from wild-type BW25113 cells, carrying the c
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4482635_pgen.1005354.g005&req=4
5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside - Creative Enzymes5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside - Creative Enzymes
Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
https://www.creative-enzymes.com/product/5Bromo4chloro3indoxyl-Dfucopyranoside_8565.html
bgaC - Beta-galactosidase - Bacillus circulans - bgaC gene & proteinbgaC - Beta-galactosidase - Bacillus circulans - bgaC gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/O31341
Western control-Beta-Galactosidase (E. Coli) Protein for Western Blot-Genemed Synthesis Inc.
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C
http://genemedsyn.com/ccpc1034-5207-beta-galactosidase-28e--coli29-protein-for-western--western-control-bgal11-c.htm
pSF-TEF1-COOH-BetaGal (OG2040) C-terminal BetaGal tag yeast plasmidpSF-TEF1-COOH-BetaGal (OG2040) C-terminal BetaGal tag yeast plasmid
C-terminal BetaGal reporter tag yeast expression plasmid. This vector allows the creation of reporter fusion proteins with no protease cleavage tag.
https://www.oxfordgenetics.com/Products/Plasmids/Details/Yeast/pSF-TEF1-COOH-BetaGal/OG2040
P - Eukaryotic Cells - GUWS MedicalP - Eukaryotic Cells - GUWS Medical
lacZ. No transcription. Structural genes lacZ. lacY. lac A. No transcription. Presence of lactose. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor). Allolactose. 12 When lactose is present, some of it is converted into allolactose,.... RNA polymerase lacO operator. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor) ...
https://www.guwsmedical.info/eukaryotic-cells/p-yie.html
AID 404864 - Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene...AID 404864 - Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene...
BioAssay record AID 404864 submitted by ChEMBL: Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene assay relative to control.
https://pubchem.ncbi.nlm.nih.gov/bioassay/404864
Old cells, new tricks: chromatin structure in senescence | SpringerLink. Senescence Assay Kit (Beta Galactosidase, Fluorescence...
Old cells, new tricks: chromatin structure in senescence | SpringerLink. Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) | Abcam. Overview of Cellular Senescence and Aging | Cell Signaling Technology. The chemistry of senescence | Nature Reviews Chemistry. Infographic: How Does Cell Senescence Drive Aging and Disease? | The Scientist Magazine®. Cellular senescence is associated with age-related blood clots. Frontiers | Cellular Senescence as the Causal Nexus of Aging | Genetics. Diabetic Retinopathy: premature cellular senescence and microvascular dysfunction - ELISA Genie. Stem Cells Versus Senescence | JACC: Journal of the American College of Cardiology. MHY2233 Attenuates Replicative Cellular Senescence in Human Endothelial Progenitor Cells via SIRT1
http://llaweb.org/senescence.html
Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose - Zurich Open Repository and...Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose - Zurich Open Repository and...
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase ...
http://www.zora.uzh.ch/id/eprint/113580/
Biblioteca Florestal - α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (Vell.) Allemão ex...Biblioteca Florestal - α-galactosidase activity and carbohydrate mobilization in seeds of Dalbergia nigra (Vell.) Allemão ex...
Substâncias complexas são convertidas, pela ação de enzimas, em moléculas solúveis, durante o processo de germinação, as quais são translocadas para a plântula em crescimento, servindo como fonte de energia ou estrutura física. Com o objetivo de quantificar a atividade enzimática da -galactosidase e a mobilização de mono e oligossacarídeos durante o período de germinação, foi conduzido este estudo utilizando-se sementes de jacarandá-da-bahia. As sementes foram mantidas em germinador a 25oC sob luz contínua, sendo avaliada a protrusão da radícula, pelo período de 10 dias. Foram quantificados os teores de mono e de oligossacarídeos, assim como a atividade específica da enzima -galactosidase nos tempos zero, um, três e cinco dias. Houve mobilização das reservas de glicose e manose nos cotilédones e xilose neste e no embrião nos três primeiros dias de germinação. A ramnose teve os teores aumentados nos cotilédones e no eixo embrionário. A rafinose foi o ...
http://www.bibliotecaflorestal.ufv.br/handle/123456789/15801
Beta-galactosidase recombinant Fab fragment - (RAB00031) - Products - Abnova
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
http://www.abnova.com/products/products_detail.asp?catalog_id=RAB00031
Detection of multiple β-galactosidase activities with β1-3 and β1-4 specific substrates | Biochemical Society TransactionsDetection of multiple β-galactosidase activities with β1-3 and β1-4 specific substrates | Biochemical Society Transactions
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://www.biochemsoctrans.org/content/17/4/745
Protein Informative Tools - Bioinformatics and BiostatisticsProtein Informative Tools - Bioinformatics and Biostatistics
You might also use the Kyoto Encyclopedia of Genes and Genomes, which has a lot of information conveniently summarized for many proteins; for example, the entry for the E. coli beta-galactosidase gene is here. Note that the entries for orthologies, pathways, motifs, cross-references to other databases, structures, etc. are all clickable.. ...
http://www.protocol-online.org/biology-forums/posts/38724.html
Browsing by Subject lactose hydrolysisBrowsing by Subject lactose hydrolysis
It was found that Concanavalin A (Con A) accelerates the rates of hydrolysis of E. coli beta-galactosidase and yeast invertase by binding to the product (glucose) formed in the reaction. The effect of Con A can be made ...
http://eprint.iitd.ac.in/browse?type=subject&value=lactose+hydrolysis
Experimental studies on the kinetics of adsorption of beta-galactosida by Barbara June HearnExperimental studies on the kinetics of adsorption of beta-galactosida by Barbara June Hearn
The adsorption of beta-galactosidase (BG) from solution by anti-beta-galactosidase monoclonal antibody (ABG) immobilized on nonporous, glass coated beads has been studied in a stirred batch adsorber at 4, 8, 15, and 20°C and for different initial BG concentrations. The elution of BG from the BG-ABG complex was also examined. The experiments performed in this work provided data from which information may be obtained about the influence of the concentration of BG and of the temperature on the dynamics of the adsorption of BG onto immobilized ABG, as well as on the total amount of BG adsorbed at equilibrium. Such experimental data may be used in studies involving the modelling of the kinetics of adsorption of BG onto immobilized ABG--Abstract, page ii.
https://scholarsmine.mst.edu/masters_theses/774/
A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta...A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta...
An optimized chemiluminescent assay for beta-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2-tricyclo-[3.3.1. 1(3,7)]decan]-4- yl)phenyl-beta-D-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of beta-gal …
https://pubmed.ncbi.nlm.nih.gov/1807155/?dopt=Abstract
AID 142597 - Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in...AID 142597 - Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in...
BioAssay record AID 142597 submitted by ChEMBL: Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in NIH 3T3 cells. Maximal effect normalized to the effect of carbachol (%CCH)..
https://pubchem.ncbi.nlm.nih.gov/bioassay/142597
Translational Control of the Antibiotic Inducibility of the PA5471 Gene Required for mexXY Multidrug Efflux Gene Expression in...Translational Control of the Antibiotic Inducibility of the PA5471 Gene Required for mexXY Multidrug Efflux Gene Expression in...
Interestingly, elimination of PA5471.1 translation via an M1T mutation also enhanced PA5471 expression, reminiscent of the effects of eliminating the pBC16 tet LP ribosome binding site (i.e., no translation of the tet gene possible) where Tet protein expression was enhanced 10-fold and was tetracycline unresponsive (29). It is unclear, however, how lack of PA5471.1 translation would provide the same signal as ribosome stalling, although in the case of the M1T mutation the ribosomes may be pausing at the ribosome binding site in the absence of a start codon.. The observation that fusion strains carrying 3′-truncated fragments of the PA5471 upstream region lacking a putative attenuator (Fig. 1, K2809, K2792, and K2794) yielded less β-galactosidase activity than full-length upstream fragments containing the attenuator sequences but carrying a nonsense mutation (Q3Am) in PA5471.1 (Fig. 1, K2800) was puzzling given the expectation that an attenuator-minus sequence would, in theory, provide for ...
https://jb.asm.org/content/191/15/4966
The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert -...The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert -...
The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert presence of insert results into insertional inactivation of Beta galactosidase and recombinant colonies do not produce any colour these are identified as Recombinant Colony
https://www.meritnation.com/ask-answer/question/the-presence-of-chromogenic-substrate-gives-blue-coloured-co/biotechnology-principles-and-processes/12519987
Identification of Molecular Determinants of Cellular Senescence in Cancer and AgingIdentification of Molecular Determinants of Cellular Senescence in Cancer and Aging
Cellular senescence is a fundamental cell fate playing significant and complex roles during tumorigenesis and natural aging process. However, the molecular determinants distinguishing senescence from other temporary and permanent cell-cycle arrest states such as quiescence and post-mitotic state and the specified mechanisms underlying cell-fate decisions towards senescence versus cell death in response to cellular stress stimuli remain less understood. In our studies, we aimed to employ multi-omics approaches to deepen our understanding of cellular senescence, in particular, regarding the specific molecular determinants distinguishing cellular senescence from other non-dividing cell fates. Notably, one of the most prominent features of cellular senescence differing from other non-dividing cell fates is the increased expression of senescence-associated beta-galactosidase. Because 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG) is known as the substrate catalyzed by ...
https://dukespace.lib.duke.edu/dspace/handle/10161/17470
Help: beta gal reporter activity unreliableHelp: beta gal reporter activity unreliable
A Q: for transfection gurus. We are measuring promoter activity using luciferase gene as a main reporter, fused to the putative promoter fragments, and CMV/beta-galactosidase gene as a second, normalizer activity. Both are introduced by lipofection into an aortic cell line. Luciferase activity is measured using a Promega kit, and the b-gal is measured via a chemiluminescence based kit from Tropix (GalactoLight). Before measuring the latter activity we heat-inactivate the endogenous activity. What we get is that for a given construct the luciferase activity in a 3X transfection series is relatively constant ( within a factor of 1.5), but the respective set of b-gal activities is variable within a factor of 3, which ruins the experiment. Any ideas? Has anyone used GalactoLight with success? -- Alexander Kraev, PhD Biochemie III, ETHZ Zurich Phone 41-1-632-31-47 Fax 41-1-632-12-13 e-mail kraev at bc.biol.ethz.ch ...
http://www.bio.net/bionet/mm/methods/1996-October/050878.html
3:42 pm3:42 pm
Using a fungus two-hybrid system, we isolated a book human centrosomal protein, CPAP (centrosomal P4. utilized to display screen for protein that connect to 4.1R-135. The top domains (HD; residues 1 to 209) of 4.1R-135 (4.1R-HD) was fused towards the GAL4 DNA-binding domains (GAL4-DB) in vector pAS2-1 (Clontech). This create was used as bait to display a Z-VAD-FMK inhibition human being lymphocyte cDNA library fused to a GAL4 activation website (GAL4-AD) in the pACT2 vector (Clontech). The two types of plasmids were then cotransformed into Y190, and the transformants were selected on SD minimal medium as previously explained (40). Positive colonies were further tested for -galactosidase activity using a colony-lift assay and liquid assay as explained by the manufacturer (Clontech). To thin down the head website region of 4.1R (4.1R-HD) that binds to CPAP, constructs containing numerous portions of 4.1R-HD were fused to GAL4-DB of the pAS2-1 vector (Fig. ?(Fig.1A).1A). The C terminus of CPAP ...
http://www.antibodyassay.com/index.php/2019/05/28/
The Briarfield Chronicles: Promoter recognitionThe Briarfield Chronicles: Promoter recognition
Dimerization data (column 1) represent relative binding to GST-TBP(181C) as determined in Figure 1E and are an average of six sets of data. See Figure 5 for standard errors. β-galactosidase activity (columns 3-6) is relative to wild-type test TBP in column 3 (1.0 = 3 Miller units using the high-sensitivity substrate CPRG) and represents an average of at least three independent determinations. All data were obtained in the linear range of the assay. The null allele expresses only the first 81 amino acids of TBP. Test HA-tagged TBP levels were determined by quantitative Western blotting with TBP antibodies as described in Figure 5B. TBP levels are relative to the endogenous (untagged) TBP (1 = 17,000 ± 2,000 molecules per cell). The standard error for all data is presented graphically in Figure 5E. Expression of the TBPEBmutants did not cause a decrease in the expression of TAFII145 (data not shown). Low in columns 3 and 7 indicates that the test TBP was driven by the TBP promoter; high in ...
http://thebriarfieldchronicles.blogspot.com/2012/11/promoter-recognition.html
4CUA UNRAVELLING THE MULTIPLE FUNCTIONS OF THE ARCHITECTURALLY INTRICATE STREPTOCOCCUS PNEUMONIAE BETA-GALACTOSIDASE, BGAA |...4CUA UNRAVELLING THE MULTIPLE FUNCTIONS OF THE ARCHITECTURALLY INTRICATE STREPTOCOCCUS PNEUMONIAE BETA-GALACTOSIDASE, BGAA |...
Fullscreen (supported by IE11, latest versions of Firefox, Chrome, Safari (not including iOS Safari), Edge, Chrome for Android, Samsung Internet) ...
https://cansarblack.icr.ac.uk/target/P15056/3d/4CUA_B
Plus itPlus it
The results of this study are consistent with the following conclusions. First, unconjugated β-galactosidase is rapidly cleared from blood in vivo (Table 2), owing to rapid uptake of the unconjugated enzyme by liver and spleen (Figs. 2 and 3). Second, once inside cells, β-galactosidase is rapidly degraded in vivo such that 99% of the organ enzyme activity is lost at 4 h after an intravenous injection (Table 1). Third, the 116-kDa β-galactosidase (Fig. 1B) can be conjugated to the 8D3 TfRmAb without loss of enzyme activity (Fig. 1C). Fourth, there is minimal brain uptake of the unconjugated β-galactosidase, but there is a 10-fold increase in brain uptake of enzyme following conjugation to the 8D3 TfRmAb (Table 1; Figs. 2 and 3).. The β-galactosidase is rapidly removed from the blood due to the avid uptake of the enzyme by liver and spleen (Figs. 2 and 3), which confirms the earlier observation of Onodera et al. (1983). The blood concentration of the β-galactosidase-TfRmAb is 5- to 10-fold ...
http://jpet.aspetjournals.org/content/313/3/1075
ATCC ® 77267™ATCC ® 77267™
ATCC ® 77267™ Designation: pUN65 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Molecular_Biology/Clones/77267.aspx?slp=1
ATCC ® 77269™ATCC ® 77269™
ATCC ® 77269™ Designation: pUN90 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
https://www.atcc.org/products/all/77269.aspx
300 Blue/White Cloning of a DNA Fragment & Assay of ß-galactosidase - - Discovering DNA
Edvotek 300 kit shows how to clone a DNA fragment by using ligation, transformation and an assay of ß-galactosidase. For advanced students.
https://discoveringdna.com/collections/genetic-engineering-transformation/products/300
β-galactosidase/ZIF-8 MOF coatingβ-galactosidase/ZIF-8 MOF coating
Page contains details about β-galactosidase/ZIF-8 MOF coating . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
http://nano.nature.com/nano/GR-M9406452
29722 P{w[+mC]=UASp-Khc::nod::lacZ}1, y[1] w[*]; In(2LR)Gla, wg[Gla-1]/SM6a29722 P{w[+mC]=UASp-Khc::nod::lacZ}1, y[1] w[*]; In(2LR)Gla, wg[Gla-1]/SM6a
Expresses the head domain of Nod fused to the coiled-coil domain of KHC and beta-galactosidase under the control of UAS; the fusion protein accumulates at the minus ends of microtubules, N.G ...
http://flystocks.bio.indiana.edu/Reports/29722.html
Difference between revisions of Beta-glucuronidase protocols - OpenWetWareDifference between revisions of Beta-glucuronidase protocols - OpenWetWare
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols. ...
https://openwetware.org/wiki/?title=Beta-glucuronidase_protocols&diff=578949&oldid=520925
Antibodies Search | ALZFORUMAntibodies Search | ALZFORUM
reacts with native and denatured-reduced E. coli β-galactosidase(116 kD); may be usedfor detection of β-galactosidase expressed by E. colilacZ gene encoded in many cloned gene sequences,and serves as an indicator for fusion proteins encodedby an inserted DNA ...
https://www.alzforum.org/antibodies/search?category%5B22541%5D=%CE%B2-Galactosidase
Beta-galactosidaseBeta-galactosidase
A new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) which is ... β-Galactosidase (EC 3.2.1.23, lactase, beta-gal or β-gal; systematic name β-D-galactoside galactohydrolase), is a glycoside ... beta-Galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (CS1: long volume ... However, it is now known that this is due to an overexpression and accumulation of the lysosomal endogenous beta-galactosidase ...
https://en.wikipedia.org/wiki/Beta-galactosidase
Endo-beta-galactosidaseEndo-beta-galactosidase
... may refer to: Blood-group-substance endo-1,4-beta-galactosidase Keratan-sulfate endo-1,4-beta- ... galactosidase This set index page lists enzyme articles associated with the same name. If an internal link led you here, you ...
https://en.wikipedia.org/wiki/Endo-beta-galactosidase
6-phospho-beta-galactosidase6-phospho-beta-galactosidase
... beta-D-phosphogalactoside galactohydrolase, phospho-beta-D-galactosidase, and 6-phospho-beta-D-galactosidase. This enzyme ... A 6-phospho-beta-galactosidase (EC 3.2.1.85) is an enzyme that catalyzes this chemical reaction: a 6-phospho-beta-D-galactoside ... Hengstenberg W, Penberthy WK, Morse ML (1970). "Purification of the staphylococcal 6-phospho-beta-D-- galactosidase". Eur. J. ... Other names in common use include phospho-beta-galactosidase, ... The systematic name of this enzyme class is 6-phospho-beta-D- ...
https://en.wikipedia.org/wiki/6-phospho-beta-galactosidase
Senescence-associated beta-galactosidaseSenescence-associated beta-galactosidase
"Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111/j.1474- ... Senescence-associated beta-galactosidase (SA-β-gal or SABG) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of ... Senescence-associated beta-galactosidase, along with p16Ink4A, is regarded to be a biomarker of cellular senescence. Its ... following the observation that when beta-galactosidase assays were carried out at pH 6.0, only cells in senescence state ...
https://en.wikipedia.org/wiki/Senescence-associated_beta-galactosidase
Galactan 1,3-beta-galactosidaseGalactan 1,3-beta-galactosidase
3-beta-galactosidase (EC 3.2.1.145, galactan (1->3)-beta-D-galactosidase) is an enzyme with systematic name galactan 3-beta-D- ... Galactan+1,3-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 3.2. ... galactosidase. This enzyme catalyses the following chemical reaction Hydrolysis of terminal, non-reducing beta-D-galactose ... residues in (1->3)-beta-D-galactopyranans This enzyme removes not only free galactose, but also 6-glycosylated residues. ...
https://en.wikipedia.org/wiki/Galactan_1,3-beta-galactosidase
Arabinogalactan endo-1,4-beta-galactosidaseArabinogalactan endo-1,4-beta-galactosidase
Arabinogalactan endo-beta-1,4-galactanase (EC 3.2.1.89, endo-1,4-beta-galactanase, galactanase, arabinogalactanase, ganB (gene ... and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer ... Arabinogalactan+endo-beta-1,4-galactanase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: ... Purification and characterization of a beta-1,4-galactanase which degrades a structural component of the primary cell walls of ...
https://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase
Galactan endo-1,6-beta-galactosidaseGalactan endo-1,6-beta-galactosidase
... (EC 3.2.1.164, endo-1,6-beta-galactanase) is an enzyme with systematic name endo-beta-(1-> ... Galactan+endo-1,6-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC ... beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products The enzyme specifically hydrolyses 1,6- ... Okemoto K, Uekita T, Tsumuraya Y, Hashimoto Y, Kasama T (January 2003). "Purification and characterization of an endo-beta-(1 ...
https://en.wikipedia.org/wiki/Galactan_endo-1,6-beta-galactosidase
Keratan-sulfate endo-1,4-beta-galactosidaseKeratan-sulfate endo-1,4-beta-galactosidase
... (EC 3.2.1.103, endo-beta-galactosidase, keratan sulfate endogalactosidase, ... Keratan-sulfate+endo-1,4-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal: ... Fukuda MN, Matsumura G (October 1976). "Endo-beta-galactosidase of Escherichia freundii. Purification and endoglycosidic action ... keratanase, keratan-sulfate 1,4-beta-D-galactanohydrolase) is an enzyme with systematic name keratan-sulfate 4-beta-D- ...
https://en.wikipedia.org/wiki/Keratan-sulfate_endo-1,4-beta-galactosidase
Blood-group-substance endo-1,4-beta-galactosidaseBlood-group-substance endo-1,4-beta-galactosidase
... (EC 3.2.1.102, endo-beta-galactosidase, blood-group-substance 1,4-beta-D- ... Blood-group-substance+endo-1,4-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Portal ... Takasaki S, Kobata A (June 1976). "Purification and characterization of an endo-beta-galactosidase produced by Diplococcus ... Fukuda MN, Matsumura G (October 1976). "Endo-beta-galactosidase of Escherichia freundii. Purification and endoglycosidic action ...
https://en.wikipedia.org/wiki/Blood-group-substance_endo-1,4-beta-galactosidase
Glycoside hydrolase family 35Glycoside hydrolase family 35
... beta-galactosidase (EC 3.2.1.23). Mammalian beta-galactosidase is a lysosomal enzyme (gene GLB1) which cleaves the terminal ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_35
Glycoside hydrolase family 1Glycoside hydrolase family 1
... beta-galactosidase (EC 3.2.1.23); 6-phospho-beta-galactosidase (EC 3.2.1.85); 6-phospho-beta-glucosidase (EC 3.2.1.86); lactase ... 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT; LCTL; GH1 in CAZypedia Henrissat B, Callebaut I, Fabrega S ... Glycoside hydrolase family 1 CAZY GH_1 comprises enzymes with a number of known activities; beta-glucosidase (EC 3.2.1.21); ... hydrolase (EC 3.2.1.62), lactase (EC 3.2.1.108); beta-mannosidase (EC 3.2.1.25); myrosinase (EC 3.2.1.147). ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_1
Rhodanobacter fulvusRhodanobacter fulvus
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ... Rhodanobacter fulvus produce beta-galactosidase. Parte, A.C. "Rhodanobacter". LPSN. "Rhodanobacter fulvus". www.uniprot.org. ...
https://en.wikipedia.org/wiki/Rhodanobacter_fulvus
Glycoside hydrolase family 16Glycoside hydrolase family 16
... endo-beta-1,3-glucanase (EC 3.2.1.39); endo-beta-1,3-1,4-glucanase (EC 3.2.1.6); endo-beta-galactosidase (EC 3.2.1.103). ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_16
RhodanobacterRhodanobacter
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ...
https://en.wikipedia.org/wiki/Rhodanobacter
GLB1L3GLB1L3
"Entrez Gene: Galactosidase, beta 1-like 3". Retrieved 2012-07-24. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, ... Galactosidase, beta 1-like 3 is a protein in humans that is encoded by the GLB1L3 gene. GRCh38: Ensembl release 89: ...
https://en.wikipedia.org/wiki/GLB1L3
Blue-white screenBlue-white screen
Langley, K. E.; Villarejo, M. R.; Fowler, A. V.; Zamenhof, P. J.; Zabin, I. (1975). "Molecular basis of beta-galactosidase ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ...
https://en.wikipedia.org/wiki/Blue–white_screen
Herbert Weissbach (biochemist)Herbert Weissbach (biochemist)
weissbach, H (1977). "DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors". The Journal of ...
https://en.wikipedia.org/wiki/Herbert_Weissbach_(biochemist)
Marker geneMarker gene
The bacterial lacZ gene encodes a beta-galactosidase enzyme. When media containing certain galactosides (e.g. X-gal), cells ...
https://en.wikipedia.org/wiki/Marker_gene
Cathepsin ACathepsin A
1991). "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases". ... 1994). "Normal serum beta-galactosidase in juvenile GM1 gangliosidosis". Pediatr. Neurol. 10 (4): 317-9. doi:10.1016/0887-8994( ... Chakraborty S, Rafi MA, Wenger DA (1994). "Mutations in the lysosomal beta-galactosidase gene that cause the adult form of GM1 ... 1991). "Combined deficiency of beta-galactosidase and neuraminidase: natural history of the disease in the first 18 years of an ...
https://en.wikipedia.org/wiki/Cathepsin_A
Glycoside hydrolase family 2Glycoside hydrolase family 2
... beta-galactosidase (EC 3.2.1.23); beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a ... The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... Jacobson RH, Zhang XJ, DuBose RF, Matthews BW (June 1994). "Three-dimensional structure of beta-galactosidase from E. coli". ... beta-galactosidase from Escherichia coli". The Journal of Biological Chemistry. 267 (16): 11126-30. doi:10.1016/S0021-9258(19) ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_2
Beta-D-fucosidaseBeta-D-fucosidase
Rodriguez JA, Cabezas JA, Calvo P (1982). "beta-Fucosidase, beta-glucosidase and beta-galactosidase activities associated in ... Wiederschain GY, Prokopenkov AA (October 1973). "Beta-D-galactosidase and beta-D-fucosidase of pig kidney". Archives of ... Beta-D-fucosidase (EC 3.2.1.38, beta-fucosidase) is an enzyme with systematic name beta-D-fucoside fucohydrolase. This enzyme ... Chinchetru MA, Cabezas JA, Calvo P (1983). "Characterization and kinetics of beta-D-gluco/fuco/galactosidase from sheep liver ...
https://en.wikipedia.org/wiki/Beta-D-fucosidase
Phenyl-D-galactopyranosidePhenyl-D-galactopyranoside
"Purification and characterization of a thermotolerant beta-galactosidase from Thermomyces lanuginosus". Applied and ...
https://en.wikipedia.org/wiki/Phenyl-D-galactopyranoside
Helen BlauHelen Blau
von Degenfeld, Georges; Wehrman, Tom S.; Blau, Helen M. (2009-01-01). Imaging beta-galactosidase activity in vivo using ... "Luminescent imaging of beta-galactosidase activity in living subjects using sequential reporter-enzyme luminescence". Nature ... Her discovery of β-galactosidase complementation is widely used in drug discovery. Non-invasive bioluminescence imaging enables ...
https://en.wikipedia.org/wiki/Helen_Blau
GalactosialidosisGalactosialidosis
This leads to a deficiency of beta-galactosidase (GLB1) and neuraminidase-1 (NEU1). As a result, the lysosome is unable to ... This gene encodes the enzyme cathepsin A, which forms a protein complex with neuraminidase-1 and beta-galactosidase to break ... Cathepsin A is required in this process because within the complex, it prevents neuraminidase-1 and beta-galactosidase from ... in 1979 by measuring beta-galactosidase and neuraminidase activities in cultured amniotic fluid cells. Because the disease is ...
https://en.wikipedia.org/wiki/Galactosialidosis
Penicillium multicolorPenicillium multicolor
ATCC Flood, M. T.; Kondo, M (2004). "Toxicity evaluation of a beta-galactosidase preparation produced by Penicillium multicolor ... "Isolation and characterization of a beta-primeverosidase-like enzyme from Penicillium multicolor". Bioscience, Biotechnology, ...
https://en.wikipedia.org/wiki/Penicillium_multicolor
Sialidase-1Sialidase-1
In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter ... Activation, stabilization and association with beta-galactosidase and its protective protein". Eur. J. Biochem. 149 (2): 315-21 ... "Association of N-acetylgalactosamine-6-sulfate sulfatase with the multienzyme lysosomal complex of beta-galactosidase, ...
https://en.wikipedia.org/wiki/Sialidase-1
LactaseLactase
... (or a similar form of beta-galactosidase) is also used to screen for blue white colonies in the multiple cloning sites ... Beta-galactosidase PDBe-KB provides an overview of all the structure information available in the PDB for Escherichia coli Beta ... beta-galactosidase". Biochemistry. 40 (49): 14781-94. doi:10.1021/bi011727i. PMID 11732897. Fernandez P, Cañada FJ, Jiménez- ... Lactase (also known as lactase-phlorizin hydrolase, or LPH), a part of the β-galactosidase family of enzymes, is a glycoside ...
https://en.wikipedia.org/wiki/Lactase
Eastern blotEastern blot
Olson and Eglen; Eglen, RM (2007). "beta Galactosidase complementation: A cell-based luminescent assay platform for drug ...
https://en.wikipedia.org/wiki/Eastern_blot
Protein-fragment complementation assayProtein-fragment complementation assay
... beta-galactosidase) Luciferase, including ReBiL (recombinase enhanced bimolecular luciferase) and Gaussia princeps luciferase. ... "Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation". Proceedings of the ... Park JH, Back JH, Hahm SH, Shim HY, Park MJ, Ko SI, Han YS (October 2007). "Bacterial beta-lactamase fragmentation ... Remy I, Ghaddar G, Michnick SW (2007). "Using the beta-lactamase protein-fragment complementation assay to probe dynamic ...
https://en.wikipedia.org/wiki/Protein-fragment_complementation_assay
François JacobFrançois Jacob
Ullmann, A.; Jacob, F.; Monod, J. (1968). "On the subunit structure of wild-type versus complemented beta-galactosidase of ... "The role of the inducible alleles and the constitutive alleles in the synthesis of beta-galactosidase in zygotes of Escherichia ... of a peptide fraction of Escherichia coli beta-galactosidase". Journal of Molecular Biology. 12 (3): 918-923. doi:10.1016/S0022 ... by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural ...
https://en.wikipedia.org/wiki/François_Jacob
Potassium ferrocyanidePotassium ferrocyanide
... is used in a mixture with potassium ferricyanide and phosphate buffered solution to provide a buffer for beta-galactosidase, ... which is used to cleave X-Gal, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal ...
https://en.wikipedia.org/wiki/Potassium_ferrocyanide
Enzyme inhibitorEnzyme inhibitor
... modeling beta-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta (BBA) - General Subjects. 1770 (5): 733- ...
https://en.wikipedia.org/wiki/Enzyme_inhibitor
P16P16
... INK4a, along with senescence-associated beta-galactosidase, is regarded to be a biomarker of cellular senescence. Therefore ... and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells". Aging. 8 (7): 1294- ...
https://en.wikipedia.org/wiki/P16
Glycoside hydrolase family 36Glycoside hydrolase family 36
Alpha-galactosidase (EC 3.2.1.22) (melibiase) catalyzes the hydrolysis of melibiose into galactose and glucose. In man, the ... Raffinose (O-alpha- D-galactopyranosyl- (1-->6)- O-alpha- D-glucopyranosyl-(1<-->2)- O-beta- D-fructofuranoside) is a ... Escherichia coli alpha-galactosidase (gene melA), which requires NAD and magnesium as cofactors, is not structurally related to ... The sequence of this enzyme is highly related to that of the eukaryotic alpha-galactosidases. This family also includes ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_36
Marc ZabeauMarc Zabeau
Zabeau M, Stanley KK., Enhanced expression of cro-beta-galactosidase fusion proteins under the control of the PR promoter of ...
https://en.wikipedia.org/wiki/Marc_Zabeau
PTPRKPTPRK
Wang SE, Wu FY, Shin I, Qu S, Arteaga CL (2005). "Transforming growth factor {beta} (TGF-{beta})-Smad target gene protein ... Using a β-galactosidase (β-gal) reporter gene inserted into the phosphatase domain of the murine PTPkappa (PTPRK) gene, Shen ... The transgenic mouse was created by combining a β-galactosidase (β-gal) reporter gene with a signal sequence and the ... PTPRK has been shown to interact with: Beta-catenin, E-cadherin (CDH-1), Epidermal growth factor receptor (EGFR), HER2, ...
https://en.wikipedia.org/wiki/PTPRK
X-galX-gal
... they form a functional β-galactosidase. The presence of an active β-galactosidase may be detected when cells are grown in ... "Purple-beta-D-Gal - PubChem". Retrieved 4 February 2014. "5-Bromo-6-chloro-3-indolyl-β-D-glucopyranoside". Retrieved 4 February ... X-gal is often used in molecular biology to test for the presence of an enzyme, β-galactosidase, in the place of its usual ... In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional β-galactosidase enzyme in a ...
https://en.wikipedia.org/wiki/X-gal
Oncolytic virusOncolytic virus
The E. coli enzymes beta-glucuronidase and beta-galactosidase can also be encoded by some viruses. These enzymes, in the ... Li H, Peng KW, Dingli D, Kratzke RA, Russell SJ (August 2010). "Oncolytic measles viruses encoding interferon beta and the ...
https://en.wikipedia.org/wiki/Oncolytic_virus
P-selectinP-selectin
Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase- ...
https://en.wikipedia.org/wiki/P-selectin
Pseudogymnoascus destructansPseudogymnoascus destructans
... alpha/beta-galactosidase, β-glucuronidase, α-fucosidase, α-mannosidase, and trypsin. Important dual virulence factors found in ...
https://en.wikipedia.org/wiki/Pseudogymnoascus_destructans
HomomericHomomeric
It is often used to describe proteins made up of multiple identical repeating polypeptide chains e.g. beta galactosidase. " ...
https://en.wikipedia.org/wiki/Homomeric
Stem cell markerStem cell marker
August 2004). "Beta-galactosidase of ROSA26 mice is a useful marker for detecting the definitive erythropoiesis after stem cell ... asialo GM1 Bcl-2 Beta-galactosidase (β-gal) of ROSA26 mice beta1-integrin bromodeoxyuridine c-kit (CD117) c-Met C1qR(p) END ( ...
https://en.wikipedia.org/wiki/Stem_cell_marker
Beta-glucuronidaseBeta-glucuronidase
Human β-glucuronidase is homologous to the Escherichia coli enzyme β-galactosidase. This homologous relationship, along with ... Beta-glucuronidase contains several notable structural formations, including a type of beta barrel known as a jelly roll barrel ... Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB ... Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β- ...
https://en.wikipedia.org/wiki/Beta-glucuronidase
GalactoseGalactose
In the cyclic form there are two anomers, named alpha and beta, since the transition from the open-chain form to the cyclic ... The hydrolysis of lactose to glucose and galactose is catalyzed by the enzymes lactase and β-galactosidase. The latter is ...
https://en.wikipedia.org/wiki/Galactose
InulinaseInulinase
It forms a beta sandwich module. The crystal structures are shown here on either side of the page. The two structures of endo- ... galactosidases, glucosidases and mannosidases". J. Am. Chem. Soc. 65 (7): 1369-1380. doi:10.1021/ja01247a029. Liu Y, Zhou SH, ... Being made of beta sheets allows the structures to be more stable during chemical reactions. Both of the inulinases have ... Inulinase contains a N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure and C ...
https://en.wikipedia.org/wiki/Inulinase
Cloning vectorCloning vector
The most widely used is the gene coding for E. coli β-galactosidase, whose activity can easily be detected by the ability of ... Antibiotic resistance is often used as marker, an example being the beta-lactamase gene, which confers resistance to the ... Cloning a fragment of DNA within the vector-based lacZα sequence of the β-galactosidase prevents the production of an active ... penicillin group of beta-lactam antibiotics like ampicillin. Some vectors contain two selectable markers, for example the ...
https://en.wikipedia.org/wiki/Cloning_vector
SNCAIPSNCAIP
"Analysis of synphilin-1 and synuclein interactions by yeast two-hybrid beta-galactosidase liquid assay". Neurosci. Lett. 325 (2 ... "Analysis of synphilin-1 and synuclein interactions by yeast two-hybrid beta-galactosidase liquid assay". Neurosci. Lett. 325 (2 ...
https://en.wikipedia.org/wiki/SNCAIP
List of diseases (N)List of diseases (N)
... folate-sensitive Neural tube defects X linked Neuraminidase beta-galactosidase deficiency Neuraminidase deficiency Neurasthenia ...
https://en.wikipedia.org/wiki/List_of_diseases_(N)
List of enzymesList of enzymes
Beta-galactosidase (EC 3.2.1.23) Hyaluronidase (EC 3.2.1.35) Function: Amylase is an enzyme that is responsible for the ... Beta-lactamase (EC 3.5.2.6) Category:EC 3.5.3 (In linear amidines) Arginase (EC 3.5.3.1) Category:EC 3.5.4 (In cyclic amidines ... 7-alpha-dihydroxy-5-beta-cholestanate-CoA ligase EC 6.2.1.29: Transferred entry: 6.2.1.7 EC 6.2.1.30: Phenylacetate-CoA ligase ... 4-olide isomerase Beta-carotene isomerase Farnesol 2-isomerase Furylfuramide isomerase Linoleate isomerase Maleate isomerase ...
https://en.wikipedia.org/wiki/List_of_enzymes
Staphylococcus nepalensisStaphylococcus nepalensis
... beta-galactosidase, and beta-glucuronidase, and hydrolyses aesculin and Tween 80. It reduces nitrate to nitrite. It is negative ... and beta-haemolysins, heat-stable and heat-labile nucleases, indole, hydrogen sulphide, and lecithinase. It produces acid ...
https://en.wikipedia.org/wiki/Staphylococcus_nepalensis
TransferaseTransferase
Earliest discoveries of transferase activity occurred in other classifications of enzymes, including beta-galactosidase, ...
https://en.wikipedia.org/wiki/Transferase
Prokaryotic large ribosomal subunitProkaryotic large ribosomal subunit
5 minutes for beta-galactosidase[citation needed]). Prokaryotic small ribosomal subunit (30S) Ribosomal RNA 23S methyl RNA ...
https://en.wikipedia.org/wiki/Prokaryotic_large_ribosomal_subunit
Staphylococcus muscaeStaphylococcus muscae
... or beta-haemeolysins, ornithine decarboxylase, acetylmethylcarbinol, or beta-galactosidase. It does not hydrolyze starch or ...
https://en.wikipedia.org/wiki/Staphylococcus_muscae
GLB1GLB1
"The chromosomal localization of human beta-galactosidase revisited: a locus for beta-galactosidase on human chromosome 3 and ... "Alternative splicing of beta-galactosidase mRNA generates the classic lysosomal enzyme and a beta-galactosidase-related protein ... Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. The GLB1 protein is a beta ... Structure-function studies of lysosomal beta-galactosidase and the non-lysosomal beta-galactosidase-like protein". Biochimica ...
https://en.wikipedia.org/wiki/GLB1
YPEL3YPEL3
... beta galactosidase staining and the appearance of senescence-associated heterochromatic foci(SAHF) within the nuclei of ...
https://en.wikipedia.org/wiki/YPEL3
3.2.1.102: blood-group-substance endo-1,4-beta-galactosidase - BRENDA Enzyme Database3.2.1.102: blood-group-substance endo-1,4-beta-galactosidase - BRENDA Enzyme Database
More, endo-beta-galactosidase, galactosidase, endo-beta-, endo-beta-galactosidase DII, blood group glycotope-cleaving endo-beta ... blood group antigen-cleaving endo-beta-galactosidase, EABase, inverting endo-beta-galactosidase, Sp3GH98, Sp4GH98, endo-beta- ... and B-cleaving endo-beta-galactosidase, E-ABase, ABase, GH98CBM51, blood group A/B antigen-specific endo-beta-galactosidase, ... 4-beta-galactosidase. This is an abbreviated version!. For detailed information about blood-group-substance endo-1,4-beta- ...
https://brenda-enzymes.org/all_enzymes.php?ecno=3.2.1.102&table=Reaction
Anti-Beta Galactosidase Antibody | CGAL-45A | Immunology Consultatnt LaboratoryAnti-Beta Galactosidase Antibody | CGAL-45A | Immunology Consultatnt Laboratory
Anti-Beta Galactosidase Antibody. Catalogue Numbre: CGAL-45A, Supplied by Immunology Consultatnt Laboratory& Distributed by ... Anti-Beta Galactosidase Antibody , CGAL-45A. Rating * Select Rating. 1 star (worst). 2 stars. 3 stars (average). 4 stars. 5 ...
https://tumori.net/anti-beta-galactosidase-antibody-cgal-45a/
BINDING-ENERGY AND CATALYSIS - FLUORINATED AND DEOXYGENATED GLYCOSIDES AS MECHANISTIC PROBES OF ESCHERICHIA-COLI (LACZ) BETA...BINDING-ENERGY AND CATALYSIS - FLUORINATED AND DEOXYGENATED GLYCOSIDES AS MECHANISTIC PROBES OF ESCHERICHIA-COLI (LACZ) BETA...
... beta-galactosidase have been determined and rates found to be two to nine orders of magnitude lower than that for the parent ... Kinetic parameters for the hydrolysis of a series of deoxy and deoxyfluoro analogues of 2,4-dinitrophenyl beta-D- ... BINDING-ENERGY AND CATALYSIS - FLUORINATED AND DEOXYGENATED GLYCOSIDES AS MECHANISTIC PROBES OF ESCHERICHIA-COLI (LACZ) BETA- ... BINDING-ENERGY AND CATALYSIS - FLUORINATED AND DEOXYGENATED GLYCOSIDES AS MECHANISTIC PROBES OF ESCHERICHIA-COLI (LACZ) BETA- ...
http://www.chem.ubc.ca/binding-energy-and-catalysis-fluorinated-and-deoxygenated-glycosides-mechanistic-probes-escherichi-0
Beta-Galactosidase Colorimetric Assay | ScienCell Research LaboratoriesBeta-Galactosidase Colorimetric Assay | ScienCell Research Laboratories
Please login or register to access the Safety Data Sheet for Beta-Galactosidase Colorimetric Assay. Certificates of Analysis ... The ScienCell β-galactosidase Colorimetric Assay provides an easy-to-use method to detect β-galactosidase by staining cells ... his assay kit is used to evaluate the β-galactosidase activity in vitro. It is for research use only. Not for use in animals, ... β-Galactosidase, an important reporter gene marker encoded by lacZ, is commonly used for monitoring transfection efficiency in ...
https://www.sciencellonline.com/beta-galactosidase-colorimetric-assay.html
IMSEAR at SEARO: Studies on the beta-D-galactosidase activity of Vibrio parahaemolyticus.
Studies on the beta-D-galactosidase activity of Vibrio parahaemolyticus. Indian Journal of Medical Research. 1972 Jun; 60(6): ...
https://imsear.searo.who.int/handle/123456789/16139
Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. :: MPG...Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. :: MPG...
Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. ... Escherichia coli beta-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets ... Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. ... Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. Applied ...
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_1878012
MedlinePlus: Genes: GMedlinePlus: Genes: G
GLB1: galactosidase beta 1. *GLDC: glycine decarboxylase. *GLI3: GLI family zinc finger 3 ... GNPTAB: N-acetylglucosamine-1-phosphate transferase subunits alpha and beta. *GNPTG: N-acetylglucosamine-1-phosphate ...
https://medlineplus.gov/genetics/gene-g/
Biology - Page 23 - Nova Science PublishersBiology - Page 23 - Nova Science Publishers
Beta-Galactosidase: Properties, Structure and Functions. $95.00. Select options. * Omega-3 Fatty Acids: Chemistry, Dietary ...
https://novapublishers.com/product-category/books/science-and-technology/life-sciences/biology/page/23/?products-per-page=48
1jz8.1 | SWISS-MODEL Template Library
E. COLI (lacZ) BETA-GALACTOSIDASE (E537Q) IN COMPLEX WITH ALLOLACTOSE ... Beta-Galactosidase. Biochemistry (2001) Release Date. 2001-12-07. Peptides. Beta-Galactosidase: ABCD. SMTL:PDB. SMTL Chain Id: ... 4 x BGC- GAL: beta-D-galactopyranose-(1-6)-beta-D-glucopyranose(Non-covalent). BGC-GAL.1: 13 residues within 4Å:*. Chain A: N. ... E. COLI (lacZ) BETA-GALACTOSIDASE (E537Q) IN COMPLEX WITH ALLOLACTOSE Coordinates. PDB Format Method. X-RAY DIFFRACTION 1.50 Å ...
https://swissmodel.expasy.org/templates/1jz8
Pseudomonas syringae produces an inhibitor of a plant defence-related Beta-galactosidase - Oxford Neuroscience
Pseudomonas syringae produces an inhibitor of a plant defence-related Beta-galactosidase ... Pseudomonas syringae produces an inhibitor of a plant defence-related Beta-galactosidase ...
https://www.neuroscience.ox.ac.uk/publications/1069783
Burkholderia fungorum Septicemia - Volume 11, Number 7-July 2005 - Emerging Infectious Diseases journal - CDC
Beta-galactosidase activity was present.. Conclusions. To the best of our knowledge, this case is the first description of ...
https://wwwnc.cdc.gov/eid/article/11/7/04-1290
Angiokeratoma Corporis Diffusum (Fabry Disease) Differential DiagnosesAngiokeratoma Corporis Diffusum (Fabry Disease) Differential Diagnoses
... is an X-linked inherited disorder caused by a deficiency of the lysosomal enzyme alpha-galactosidase. This inborn error of ... Adult-type beta-galactosidase deficiency * Aspartylglycosaminuria * Adult-onset variant of alpha- N-acetylgalactosaminidase ... Quantification of alpha-galactosidase activity in intact leukocytes. Clin Chim Acta. 2010 Jun 25. [QxMD MEDLINE Link]. ... Angiokeratoma corporis diffusum in human beta-mannosidosis: Report of a new case and a novel mutation. J Am Acad Dermatol. 2007 ...
https://emedicine.medscape.com/article/1102964-differential
PDB 6EUF | Chain THE GH43, BETA 1,3 GALACTOSIDASE, BT0265 | 6EUF A | 3D Structure | canSARSPDB 6EUF | Chain THE GH43, BETA 1,3 GALACTOSIDASE, BT0265 | 6EUF A | 3D Structure | canSARS
3D Structure of chain 6EUF_A - THE GH43, BETA 1,3 GALACTOSIDASE, BT0265 - 6EUF , canSARS
https://corona-2.cansar.icr.ac.uk/structure/6EUF/3d/6EUF_A
Browse ORF cDNA clones by species Pan troglodytes, letter g, page 1Browse ORF cDNA clones by species Pan troglodytes, letter g, page 1
galactosidase beta 1 like. protein-coding. GPATCH2. G-patch domain containing 2. protein-coding. ...
https://www.genscript.com/gene/9598/pan-troglodytes?search=g
Escherichia coli nucleoside diphosphate kinase mutants depend on translesion DNA synthesis to prevent mutagenesisEscherichia coli nucleoside diphosphate kinase mutants depend on translesion DNA synthesis to prevent mutagenesis
beta-Galactosidase / metabolism Substances * DNA, Bacterial * Escherichia coli Proteins * Uracil * Nucleoside-Diphosphate ...
https://pubmed.ncbi.nlm.nih.gov/21725024/
COUPLING OF TETRAHYMENA RIBOSOMAL-RNA SPLICING TO BETA-GALACTOSIDASE EXPRESSION IN ESCHERICHIA-COLI | CU Experts | CU BoulderCOUPLING OF TETRAHYMENA RIBOSOMAL-RNA SPLICING TO BETA-GALACTOSIDASE EXPRESSION IN ESCHERICHIA-COLI | CU Experts | CU Boulder
COUPLING OF TETRAHYMENA RIBOSOMAL-RNA SPLICING TO BETA-GALACTOSIDASE EXPRESSION IN ESCHERICHIA-COLI Journal Article *Overview ...
https://experts.colorado.edu/display/pubid_31180
Open Access@KRIBB: Oligonucleotide-directed mutagenesis of two methionine residues in recombinant beta-galactosidase-preS2...Open [email protected]: Oligonucleotide-directed mutagenesis of two methionine residues in recombinant beta-galactosidase-preS2...
Oligonucleotide-directed mutagenesis of two methionine residues in recombinant beta-galactosidase-preS2 fusion proteins. Author ... Oligonucleotide-directed mutagenesis of two methionine residues in recombinant beta-galactosidase-preS2 fusion proteins. ... E. coll JM109 cells harboring pCTHB20 plasmid overproduced ^-galactosidase-preS2 (/9gal-preS2) fusion proteins with a yield of ...
https://oak.kribb.re.kr/handle/201005/3022
IA: Knights Vindicant - + LIBER ASTARTES + - The Bolter and ChainswordIA: Knights Vindicant - + LIBER ASTARTES + - The Bolter and Chainsword
The likelihood is that, on Beta Anphelion IV, there was a captive norn-queen, since the facility was designed to discover ways ... The likelihood is that, on Beta Anphelion IV, there was a captive norn-queen, since the facility was designed to discover ways ... I would dispute this - the Nid population on Beta Anphelion exploded between their apparent escape from the lab and Inquisitor ... the population on Beta Anphelion skyrocketed despite the non-presence of Hive ships that are normally used for reproduction. ...
https://bolterandchainsword.com/topic/290411-ia-knights-vindicant/
Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis...Biosafety of biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis...
Beta-galactosidase assay. To measure promoter activity in Synechocystis, we made modifications to a previously described beta- ... S2D). To understand the characteristics of the PnrsB-RBS genetic element, we set out to analyze it using the beta-galactosidase ... We have contributed to the cyanobacterial toolbox by analyzing PnrsB-RBS (a nrsB promoter variant) with beta-galactosidase ... Evaluation of promoter activity by beta-galactosidase reporter analysis, represented as mean standardized MU (μmol ONP min−1 μg ...
https://journals.biologists.com/bio/article/5/4/519/1660/Biosafety-of-biotechnologically-important
A MIXTURE OF VEGETABLE EXTRACTS (CHAMOMILLE, PASSIONFLOWER, CARAWAY, FENNEL), AND ENZYMES (BETA-GALACTOSIDASE) FOR IRRITABLE...
A MIXTURE OF VEGETABLE EXTRACTS (CHAMOMILLE, PASSIONFLOWER, CARAWAY, FENNEL), AND ENZYMES (BETA-GALACTOSIDASE) FOR IRRITABLE ... A MIXTURE OF VEGETABLE EXTRACTS (CHAMOMILLE, PASSIONFLOWER, CARAWAY, FENNEL), AND ENZYMES (BETA-GALACTOSIDASE) FOR IRRITABLE ... A MIXTURE OF VEGETABLE EXTRACTS (CHAMOMILLE, PASSIONFLOWER, CARAWAY, FENNEL), AND ENZYMES (BETA-GALACTOSIDASE) FOR IRRITABLE ... A MIXTURE OF VEGETABLE EXTRACTS (CHAMOMILLE, PASSIONFLOWER, CARAWAY, FENNEL), AND ENZYMES (BETA-GALACTOSIDASE) FOR IRRITABLE ...
https://pure.unipa.it/it/publications/a-mixture-of-vegetable-extracts-chamomille-passionflower-caraway-
Team:Purdue/Modeling - 2008.igem.orgTeam:Purdue/Modeling - 2008.igem.org
Modeling the cleavage of X-gal by Beta-galactosidase. Assumptions * The bacteria have been on the X-gal plate sufficiently long ... This image shows the time it takes to complete cleave the X-Gal in the cell when a new beta-galactosidase molecule is ... This image shows the time it takes to complete cleave the X-Gal in the cell when a new beta-galactosidase molecule is ... This image shows the time it takes to complete cleave the X-Gal in the cell when a new beta-galactosidase molecule is ...
https://2008.igem.org/wiki/index.php?title=Team:Purdue/Modeling&oldid=101021
Elimination of senescent cells by β-galactosidase-targeted prodrug attenuates inflammation and restores physical function in...Elimination of senescent cells by β-galactosidase-targeted prodrug attenuates inflammation and restores physical function in...
Herein, we developed a prodrug strategy to design a new compound based on the increased activity of lysosomal β-galactosidase ( ... Senescence-associated beta-galactosidase is lysosomal beta-galactosidase. Aging Cell 5, 187-195 (2006). ... Bursuker, I., Rhodes, J. M. & Goldman, R. Beta-galactosidase-an indicator of the maturational stage of mouse and human ... Bolon, B. Whole mount enzyme histochemistry as a rapid screen at necropsy for expression of beta-galactosidase (LacZ)-bearing ...
https://www.nature.com/articles/s41422-020-0314-9?error=cookies_not_supported&code=e2e9eeba-04fd-4704-b808-44de61a0a46a
Results for Secondary antibodies > Mouse | Abcam: antibodies, proteins,...Results for 'Secondary antibodies > Mouse' | Abcam: antibodies, proteins,...
Take a look at our BETA site and see what weve done so far. ... Switch on our new BETA site. Now available. Search and browse ...
https://www.abcam.com/products?selected.classification=Secondary+antibodies&selected.reactivity=Mouse
KEGG T00126: SAVERM 1760
3.2.1.23 beta-galactosidase. SAVERM_1760 (bga4). BRITE hierarchy. SSDB. Ortholog. Paralog. Gene cluster. GFIT. ...
https://www.genome.jp/entry/sma:SAVERM_1760+sma:SAVERM_2095+sma:SAVERM_7479
Biblio | Page 30 | Linus Pauling Institute | Oregon State UniversityBiblio | Page 30 | Linus Pauling Institute | Oregon State University
beta-Galactosidase. Wang R, Yu Z, Sunchu B, Shoaf J, Dang I, Zhao S, Caples K, Bradley L, Beaver LM, Ho E et al.. 2017. ...
https://lpi.oregonstate.edu/biblio?f%5Bauthor%5D=636&o=asc&page=29&s=keyword
Sol Genomics Network
beta-galactosidase. arabidopsis. blastx. At1G44000.1. 68.9. 4e-12. 64. 150 bp (37.3%). 1. ...
https://www.sgn.cornell.edu/search/unigene.pl?unigene_id=SGN-U337637
SCOPe 2.08: Domain d1jywb5: 1jyw B:334-625SCOPe 2.08: Domain d1jywb5: 1jyw B:334-625
PDB Compounds: (B:) beta-galactosidase. SCOPe Domain Sequences for d1jywb5:. Sequence; same for both SEQRES and ATOM records: ( ... Protein beta-Galactosidase, domain 3 [51510] (3 species). *. Species Escherichia coli [TaxId:562] [51511] (46 PDB entries). ... Fold c.1: TIM beta/alpha-barrel [51350] (34 superfamilies). contains parallel beta-sheet barrel, closed; n=8, S=8; strand order ... PDB Description: e. coli (lacz) beta-galactosidase (e537q) in complex with pnpg ...
http://scop.berkeley.edu/sunid=67519
Labeling & detectionLabeling & detection
beta-amyloid (1) Beta-Galactosidase (2) Biotin (11) BSA (5) Calcein (5) ...
https://www.anaspec.com/en/catalog/c111~labeling--detection?catalog_product.ProductAttribute.222=20699
Search Results For Health And Wellness: Dissolves Results found: 16Search Results For Health And Wellness: Dissolves Results found: 16
Studies on the production of beta galactosidase by lactobacillus sporogenes. Properties and application of beta galactosidase. ... beta galactosidase), which improves lactose digestion and tolerance. Furthermore, in a randomized, controlled clinical trial, ...
https://vitanetonline.com/forums/search/Dissolves/1
MAK3 Antibody (NBP1-70631): Novus BiologicalsMAK3 Antibody (NBP1-70631): Novus Biologicals
Cited in 2 publications. View Rabbit Polyclonal anti-MAK3 Antibody (NBP1-70631). Validated Applications: WB, IHC. Validated Species: Human. Sample size available.
https://www.novusbio.com/products/mak3-antibody_nbp1-70631
EscherichiaDeficiencyLacZAssay kitLactaseGenesProteinLysosomalENZYMESGangliosidosisPlasmidAlkalineActivitySignificantly increasedExpressionCellsProductAlpha1.52LevelBreakHumanAlpha-galactosidaseSenescence-associatedLacZLactoseSubstrateGLB1GeneCOLIAntibodyProteinsHydrolysisXylanaseHydrolaseStaining KitGalactoseSpeciesAssayEnzymesEnzymatic activityMolecularBacterial enzymeReporterEssential enzymeInducerBacteriaPhenylethyl beta-D-thiogalactopyranosideVitroSignificantlyAgalsidase
Escherichia4
  • Kinetic parameters for the hydrolysis of a series of deoxy and deoxyfluoro analogues of 2',4'-dinitrophenyl beta-D-galactopyranoside by Escherichia coli (lacZ) beta-galactosidase have been determined and rates found to be two to nine orders of magnitude lower than that for the parent compound. (ubc.ca)
  • Escherichia coli beta-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. (mpg.de)
  • XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. (microbiologyresearch.org)
  • β-Galactosidase (Escherichia coli) ist ein tetrameres Enzym von historischer und wissenschaftlicher Bedeutung, das in mehreren molekularbiologischen Anwendungen verwendet wird. (skinsandbones.de)
Deficiency2
  • There his team described leucocyte beta-galactosidase deficiency in GM1 gangliosidosis. (childneurologysociety.org)
  • Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. (bvsalud.org)
LacZ2
  • β-Galactosidase, an important reporter gene marker encoded by lacZ, is commonly used for monitoring transfection efficiency in cultured cells and identifying expression of recombinant fusion genes. (sciencellonline.com)
  • A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. (jax.org)
Assay kit1
  • his assay kit is used to evaluate the β-galactosidase activity in vitro. (sciencellonline.com)
Lactase1
  • People with lactose intolerance lack the enzyme, beta-galactosidase or lactase, to break down lactose into glucose and galactose during digestion. (fda.gov)
Genes1
  • These included a reduction in the expression of the genes beta-galactosidase and p16, which are markers of cellular aging. (medicalnewstoday.com)
Protein1
  • Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone). (origene.com)
Lysosomal2
  • Herein, we developed a prodrug strategy to design a new compound based on the increased activity of lysosomal β-galactosidase (β-gal), a primary characteristic of senescent cells. (nature.com)
  • To specifically target senescent cells, we focused on one primary characteristic of senescent cells - the increased activity of lysosomal β-galactosidase, exploited as senescence-associated β-galactosidase (SA-β-gal). (nature.com)
ENZYMES1
  • A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. (bvsalud.org)
Gangliosidosis1
  • La deficiencia de beta-galactosidasa A1 puede causar la GANGLIOSIDOSIS GM1. (bvsalud.org)
Plasmid1
  • E. coll JM109 cells harboring pCTHB20 plasmid overproduced ^-galactosidase-preS2 (/9gal-preS2) fusion proteins with a yield of 50% of total cellular proteins (Choi et al. (kribb.re.kr)
Alkaline1
  • Beta-glucuronidase, beta-galactosidase, alkaline phosphodiesterase, and aminopeptidase were unchanged and NAD nucleosidase was markedly decreased after LPS treatment. (aai.org)
Activity8
  • IMSEAR at SEARO: Studies on the beta-D-galactosidase activity of Vibrio parahaemolyticus. (who.int)
  • Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. (mpg.de)
  • All three lines carrying the 6-kb PSA promoter showed lateral prostate-specific beta-galactosidase activity. (eur.nl)
  • Upon castration, beta-galactosidase activity rapidly declined. (eur.nl)
  • The activity of the mucosal beta-galactosidase of caecum and colon is low in both germfree and conventional rats. (eurekamag.com)
  • beta-Galactosidase activity occurs also in the chymus of germfree rats. (eurekamag.com)
  • Moreover, a lactose-containing diet alters the metabolic activity of intestinal microorganisms (activity of microbial beta-galactosidase, acidification and lowering of ph in the chymus, production of hydrogen, proteolytic activity. (eurekamag.com)
  • The assay (the SOS umu test) was based on the test agent inducing beta-galactosidase (GAL) activity in Salmonella- typhimurium strain (TA-1535/pSK1002). (cdc.gov)
Significantly increased1
  • The glass microfibers significantly increased lactate-dehydrogenase (LDH) and beta-galactosidase (BG) release in a concentration dependent manner, whereas the insulation fiberglass did not increase LDH or BG. (cdc.gov)
Expression1
  • 3. Beta galactosidase expression determined. (igem.org)
Cells1
  • The ScienCell β-galactosidase Colorimetric Assay provides an easy-to-use method to detect β-galactosidase by staining cells with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at pH 7.4. (sciencellonline.com)
Product1
  • Conclusions: Due to to the combination of chamomile, fennel, caraway, melissa, passionflower and beta-galactosidase, the product Spasmicol® turned out to be remarkedly effective in treating IBS symptoms. (unipa.it)
Alpha1
  • Alpha/beta hydrolase family [Interproscan]. (ntu.edu.sg)
1.521
  • Kcat was calculated at 1.52*10^(-20) moles X-Gal / molecule beta-Gal*min. (igem.org)
Level1
  • The uninduced level of beta-galactosidase in the cell is 10 molecules. (igem.org)
Break1
  • In Krabbe disease, an enzyme needed to break down lipids, called cerebroside beta-galactosidase, is not working correctly. (msdmanuals.com)
Human1
  • Angiokeratoma corporis diffusum in human beta-mannosidosis: Report of a new case and a novel mutation. (medscape.com)
Alpha-galactosidase4
  • 6. The medium as claimed in claim 5, wherein said substrate for the metabolic activity is specific for E. coli and is selected from a substrate for beta-glucuronidase, beta-galactosidase or alpha-galactosidase, for acidification of lactose, or for tryptophanase, beta-ribosidase, phosphatase, L-alanine aminopeptidase or L-leucine aminopeptidase. (freepatentsonline.com)
  • 8. The medium as claimed in claim 7, wherein said substrate for the metabolic activity is specific for Salmonella and is selected from a substrate for alpha-galactosidase, esterase or acidification of glucuronate, or sorbitol, propanediol, melibiose or mannitol. (freepatentsonline.com)
  • Agalsidase beta is used in the treatment of Fabry disease (a deficiency of alpha-galactosidase A enzyme). (uofmhealth.org)
  • Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12. (wikidata.org)
Senescence-associated7
  • Senescent cells display senescence-associated expression of beta galactosidase activity. (abcam.com)
  • The number of senescence-associated-β-galactosidase positive cells ( left panel ) cleary increases 72h after 8 Gy γ irradiation. (abcam.com)
  • Metformin and alpha-mangostin significantly increased cell viability, decreased reactive oxygen species, and senescence-associated beta-galactosidase in HUVECs incubated in metabolic memory condition. (greenmedinfo.com)
  • Furthermore, p-cresol and indoxyl sulfate gradually increased senescence-associated beta-galactosidase positive cells while upregulated the expression of p21 which participate in senescent process. (medsci.org)
  • The results were similar, with an added bonus - a decrease in the expression of two genes which are typical indicators of aging: senescence-associated beta-galactosidase and p16. (wallstreetpit.com)
  • Finally, cell senescence, as assessed by senescence-associated beta-galactosidase, demonstrated significantly more staining in the IGFBP-rP1/mac25 cells than control cells. (monash.edu)
  • To this end, prostate sections of the various genotypes were analyzed by senescence-associated beta-galactosidase staining (SA-gal). (cura-cancer.com)
LacZ9
  • The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ . (abcam.com)
  • An allolactose trapped at the lacZ β-galactosidase active site with its galactosyl moiety in a (4)H3 conformation provides insights into the formation, conformation, and stabilization of the transition state. (nih.gov)
  • To construct the BacCMV- LacZ cassette, hp 70/nuclacZ plasmid [ 18 ] was digested with Hind III and Xba I to remove the hp 70 promoter, and the pCMV beta-gal (Stratagene) Nsi I- Nhe I fragment containing CMV promoter was inserted in its place upstream of the LacZ gene. (medscape.com)
  • The inserted LacZ gene was detected using beta-galactosidase expression with X-gal substrate. (medscape.com)
  • Expression of lacZ was assayed by histochemical staining for Escherichia coli beta-galactosidase activity in ES cells and in differentiated derivatives obtained by teratocarcinoma formation. (nih.gov)
  • Both targeting strategies insert a bacterial beta-galactosidase reporter (LacZ) and a neomycin resistance selection cassette. (nih.gov)
  • Mannheimia succiniciproducens, a rumen bacterium belonging to the family Pasteurellaceae, has two putative beta-galactosidase genes, bgaA and bgaB, encoding polypeptides whose deduced amino acid sequences share 56% identity with each other and show approximately 30% identity to the Escherichia coli gene for LacZ. (embl.de)
  • You have the lacZ gene, which codes for an enzyme called beta-galactosidase, which is a cytoplasmic enzyme that cleaves lactoce into glucose and galactose. (khanacademy.org)
  • To study the effect of the transcription activator on a target gene (for example, beta-galactosidase, LacZ) specifically in liver, MUP-tTA mice would be mated with transgenic mice in which the TAg Target gene was controlled by the E.Coli Tetracycline Operator (Tet-O). (nih.gov)
Lactose4
  • Beta-galactosidase, which breaks down lactose into glucose and galactose. (brainscape.com)
  • Taken together, our results suggest that the beta-galactosidase paralogues of M. succiniciproducens BgaA and BgaB play a critical role in lactose metabolism and in an unknown but likely specific function for rumen bacteria, respectively. (embl.de)
  • So then you have lactose permease, which allows more lactose to enter the cell, and then you have more beta-galactosidase which can break down the lactose into galactose and glucose to be used for the cells basic metabolic needs. (khanacademy.org)
  • Coli with IPTG, and Comparision from the Amount Beta-galactosidase produced with Lactose, IPTG and Antibiotic. (bclforge.com)
Substrate9
  • Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase. (abcam.com)
  • β-Nap-β-D-Gal MOLECULAR BIOLOGY GRADE White to off-white crystalline powder 2-Naphthyl-beta-D-galactopyranoside is a substrate for beta-galactosidase. (gbiosciences.com)
  • 4-Aminophenyl-β-D-gal MOLECULAR BIOLOGY GRADE 4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. (gbiosciences.com)
  • 4-Nitrophenyl-β-D- galactosidase p-Nitrophenyl-β-D-galactoside PNP-β-D-Gal MOLECULAR BIOLOGY GRADE White to off white crystalline powder Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. (gbiosciences.com)
  • N-Methylindoxyl-β-D- galactopyranoside GreenTM-β-D-galactoside 1-Methyl-3-indoxyl-β-D-galactopyranoside GreenTM-β-D-Gal MOLECULAR BIOLOGY GRADE Off white to yellowish crystalline powder Chromogenic substrate for β-D-galactosidase, yielding a gre. (gbiosciences.com)
  • Phenyl-β-D- galactopyranoside Phenyl-β-D-galactoside Ph-β-D-Gal MOLECULAR BIOLOGY GRADE White to off white crystalline powder Phenyl-β-D-galactopyranoside is a noninducing β-galactosidase substrate. (gbiosciences.com)
  • X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate. (gbiosciences.com)
  • The galactoside glycoside 3b was assayed as substrate for in vitro beta-galactosidase enzymatic activity showing strong absorbance after releasing of the azoic chromophore. (embl.de)
  • The expression of Nol1 gene during development was determined by whole mount histochemical staining by beta-galactosidase substrate X-gal. (irb.hr)
GLB11
  • It is caused by mutations in the GLB1 gene, which encodes an enzyme called beta-galactosidase necessary for recycling of a molecule (GM1 ganglioside) in neurons. (auburn.edu)
Gene5
  • Agalsidase beta (Fabrazyme) is manufactured by Genzyme Corporation (Cambridge, Mass) and is based on expression of the human GLA gene in CHO cells. (medscape.com)
  • Indicated for adults in whom a diagnosis of Fabry disease has been confirmed and who possess an amenable galactosidase-α gene ( GLA ) variant (as determined through in vitro assay data). (medscape.com)
  • Would something like one of the blue-fluorescent proteins work, or would it best to use beta-galactosidase or another gene as the reporter? (bio.net)
  • a gene - It codes for transacetylase which assists the enzyme beta-galactosidase. (moorejustinmusic.com)
  • To investigate the physiological role(s) of LYVE-1, we generated mice in which the gene for the receptor was inactivated by replacement with a beta-galactosidase reporter. (ox.ac.uk)
COLI3
  • The repression of constitutive beta-galactosidase in Escherichia coli by glucose and other carbon sources. (wikidata.org)
  • Regulation of enzymatic activity in the intact cell: the beta-D-galactosidase of Escherichia coli. (wikidata.org)
  • Galactose stimulation of beta-galactosidase induction in galactokinaseless mutants of Escherichia coli. (wikidata.org)
Antibody1
  • Goat Anti-Mouse Ig-β-galactosidase antibody with minimal reactivity to human proteins for use in ELISA and immunohistochemistry / immunocytochemistry assays. (southernbiotech.com)
Proteins1
  • Biochemical characterization of the recombinant proteins revealed that MsBgaA is more efficient than MsBgaB in hydrolyzing o-nitrophenyl-beta-d-galactopyranoside and p-nitrophenyl-beta-d-galactopyranoside. (embl.de)
Hydrolysis4
  • Catalysis of the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. (ntu.edu.sg)
  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D- galactosides. (cathdb.info)
  • A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. (nih.gov)
  • Bacteria tested positive for beta-glucosidase and reduction of nitrate to nitrite, but negative on most tests, including indol production, urease, glucose fermentation, gelatin hydrolysis, and beta-galactosidase. (kenyon.edu)
Xylanase2
  • Dezember 2013 zur Zulassung einer Zubereitung von Endo-1,4-beta-Xylanase aus Aspergillus niger (CBS 109.713) und Endo-1,4-beta-Glucanase aus Aspergillus niger (DSM 18404) als Zusatzstoff in Futtermitteln für. (efta.int)
  • Besides ß-mannannase enzyme activity, it contains abundant of hemi-cellulase activities like xylanase, beta-glucanase, and etc. β-Mannanase degrades ß-mannan and other NSP in feed, thus, reverses their negat. (puyerinternationalgroup.com)
Hydrolase1
  • Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. (abcam.com)
Staining Kit2
  • This product is manufactured by BioVision, an Abcam company and was previously called K802 Beta-Galactosidase Staining Kit. (abcam.com)
  • On the last day, cell viability by MTT assay, oxidative stress by fluorimetric assay, the number of senescent cells by SA beta-galactosidase staining kit, and secretory interleukin-6 by ELISA kit were measured. (greenmedinfo.com)
Galactose1
  • It is also a weak β-D-galactosidase inducer and can be used in affinity chromatography for isolation of galactose-binding. (gbiosciences.com)
Species2
  • QuadFERM+ also includes other differential tests-deoxyribonuclease, DNase, and beta-lactamase tests-for Neisseria and related species. (cdc.gov)
  • To date, considerable research efforts have been devoted to characterization of new cold-active beta-D-galactosidases which were isolated directly from selected species of bacteria and yeasts, as well as with the use of metagenomic approaches. (mostwiedzy.pl)
Assay1
  • The assay (the SOS umu test) was based on the test agent inducing beta-galactosidase (GAL) activity in Salmonella- typhimurium strain (TA-1535/pSK1002). (cdc.gov)
Enzymes1
  • some animal enzymes also hydrolyze beta-D-fucosides and beta-D-glucosides (cf. (cathdb.info)
Enzymatic activity1
  • BACKGROUND: Phenylazonaphtol-beta-D-O-glycosides are alternative substrates for the detection of enzymatic activity of beta-glycosidases which are involved in various important processes. (embl.de)
Molecular3
  • In the study, using about 40,000 molecular images, the researchers were able to compute a 2.2 Å resolution map of the structure of beta-galactosidase bound to PETG. (nih.gov)
  • Synthesis of phenylazonaphtol-beta-D-O-glycosides, evaluation as substrates for beta-glycosidase activity and molecular studies. (embl.de)
  • beta-Galactosidase-instructed formation of molecular nanofibers and a hydrogel. (embl.de)
Bacterial enzyme1
  • To get an idea of just how far cryo-EM has come over the last decade, take a look at the composite image above, which shows a bacterial enzyme (beta-galactosidase) bound to a drug-like molecule (phenylethyl beta-D-thiogalactopyranoside). (nih.gov)
Reporter1
  • Injection of empty vector or vector encoding for shRNA against beta-galactosidase reporter will be used as control. (fapesp.br)
Essential enzyme1
  • Beta galactosidase is an essential enzyme in the human body. (abcam.com)
Inducer4
  • and a beta-glucosidase or cellobiosidase inducer. (freepatentsonline.com)
  • and a beta-glucosidase or cellobiosidase inducer, said inducer being a carbohydrate constituted of a carbohydrate linked in the β-position to glucose, or a carbohydrate with a β glucoside subunit. (freepatentsonline.com)
  • 2. The medium as claimed in claim 1, wherein said beta-glucosidase or cellobiosidase inducer is at a concentration of between 100 ng/l and 10 g/l. (freepatentsonline.com)
  • 3. The medium as claimed in claim 1, wherein said beta-glucosidase or cellobiosidase inducer is cellobiose. (freepatentsonline.com)
Bacteria1
  • Which beta-hemolytic bacteria is bacitracin resistant? (studystack.com)
Phenylethyl beta-D-thiogalactopyranoside1
  • the drug to which it was bound is an inhibitor called phenylethyl-beta-D-thiogalactopyranoside (PETG), which fits into a pocket in the enzyme. (nih.gov)
Vitro2
  • Glucagon-like peptide-1 (GLP-1) and its derivatives can induce beta cell neogenesis in vitro and beta cell mass expansion in vivo, but GLP-1 signalling has not been shown to regulate cell fate decisions in vivo. (springer.com)
  • Among the many humoral factors that regulate pancreas development, glucagon-like peptide-1 (GLP-1) and its analogue, exendin-4, have been reported to affect beta cell differentiation under in vitro and ex vivo conditions [ 7 - 9 ]. (springer.com)
Significantly1
  • The glass microfibers significantly increased lactate-dehydrogenase (LDH) and beta-galactosidase (BG) release in a concentration dependent manner, whereas the insulation fiberglass did not increase LDH or BG. (cdc.gov)
Agalsidase1
  • Agalsidase beta may also be used for purposes other than those listed here. (uofmhealth.org)
MedlinePlus: Genes: G
MedlinePlus: Genes: G (medlineplus.gov)
Mucopolysaccharidosis type IV: MedlinePlus Medical Encyclopedia
Mucopolysaccharidosis type IV: MedlinePlus Medical Encyclopedia (medlineplus.gov)
Biology - Page 23 - Nova Science Publishers
Biology - Page 23 - Nova Science Publishers (novapublishers.com)
GM1 gangliosidosis: MedlinePlus Genetics
GM1 gangliosidosis: MedlinePlus Genetics (medlineplus.gov)
1jz8.1 | SWISS-MODEL Template Library
1jz8.1 | SWISS-MODEL Template Library (swissmodel.expasy.org)
Browse ORF cDNA clones by species Pan troglodytes, letter g, page 1
Browse ORF cDNA clones by species Pan troglodytes, letter g, page 1 (genscript.com)
Recently Published GRAS Notices and FDA Letters | FDA
Recently Published GRAS Notices and FDA Letters | FDA (fda.gov)
Results for 'Secondary antibodies > Mouse' | Abcam: antibodies, proteins,...
Results for 'Secondary antibodies > Mouse' | Abcam: antibodies, proteins,... (abcam.com)
Sol Genomics Network
Sol Genomics Network (sgn.cornell.edu)
SCOPe 2.08: Domain d1jywb5: 1jyw B:334-625
SCOPe 2.08: Domain d1jywb5: 1jyw B:334-625 (scop.berkeley.edu)
SCOPe 2.08: Domain d1f49g1: 1f49 G:220-333
SCOPe 2.08: Domain d1f49g1: 1f49 G:220-333 (scop.berkeley.edu)
SCOPe 2.08: Domain d3i3bd5: 3i3b D:731-1023
SCOPe 2.08: Domain d3i3bd5: 3i3b D:731-1023 (scop.berkeley.edu)
SCOPe 2.08: Domain d1jz0c2: 1jz0 C:626-730
SCOPe 2.08: Domain d1jz0c2: 1jz0 C:626-730 (scop.berkeley.edu)
SCOPe 2.08: Domain d1bgmo4: 1bgm O:731-1023
SCOPe 2.08: Domain d1bgmo4: 1bgm O:731-1023 (scop.berkeley.edu)
SCOPe 2.08: Domain d3i3ed5: 3i3e D:731-1023
SCOPe 2.08: Domain d3i3ed5: 3i3e D:731-1023 (scop.berkeley.edu)
SCOPe 2.08: Domain d1jz1i2: 1jz1 I:626-730
SCOPe 2.08: Domain d1jz1i2: 1jz1 I:626-730 (scop.berkeley.edu)
Mucopolysaccharidoses Types I-VII: Practice Essentials, Pathophysiology, Epidemiology
Mucopolysaccharidoses Types I-VII: Practice Essentials, Pathophysiology, Epidemiology (emedicine.medscape.com)
Profiles Collection: The Sol Spiegelman Papers - Sol Spiegelman - Profiles in Science Search Results
Profiles Collection: The Sol Spiegelman Papers - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
NLMID: 101584919X39 - Sol Spiegelman - Profiles in Science Search Results
NLMID: 101584919X39 - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Creator: Spiegelman, Sol, 1914-1983 - Sol Spiegelman - Profiles in Science Search Results
Creator: Spiegelman, Sol, 1914-1983 - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Sol Spiegelman - Profiles in Science Search Results
Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Sol Spiegelman - Profiles in Science Search Results
Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Genre: Articles - Sol Spiegelman - Profiles in Science Search Results
Genre: Articles - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Sol Spiegelman - Profiles in Science Search Results
Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results
Publisher: National Academy of Sciences (U.S.) - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results
Exhibit Tags: rna - Sol Spiegelman - Profiles in Science Search Results (profiles.nlm.nih.gov)