beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Galactosidases: A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.alpha-Galactosidase: An enzyme that catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-galactosides including galactose oligosaccharides, galactomannans, and galactolipids.Complement C3c: A 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by COMPLEMENT FACTOR I into C3c (749-954), and C3dg (955-1303) in the presence COMPLEMENT FACTOR H.Cerebrosides: Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)Lactose: A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.Galactose: An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.alpha-L-Fucosidase: An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.Fabry Disease: An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.Nigericin: A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)Glycoside HydrolasesGlycosides: Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)Cell Aging: The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Interleukin-1beta: An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.beta 2-Microglobulin: An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Receptors, Adrenergic, beta: One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.Integrin beta3: An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.Natural History: A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)Leeches: Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.Telefacsimile: A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.Otolaryngology: A surgical specialty concerned with the study and treatment of disorders of the ear, nose, and throat.Forensic Genetics: The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.New Zealand: A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)Vaccines, Marker: Vaccines used in conjunction with diagnostic tests to differentiate vaccinated animals from carrier animals. Marker vaccines can be either a subunit or a gene-deleted vaccine.Phosphorylase b: The inactive form of GLYCOGEN PHOSPHORYLASE that is converted to the active form PHOSPHORYLASE A via phosphorylation by PHOSPHORYLASE KINASE and ATP.Molecular Weight: The sum of the weight of all the atoms in a molecule.Phosphorylases: A class of glucosyltransferases that catalyzes the degradation of storage polysaccharides, such as glucose polymers, by phosphorolysis in animals (GLYCOGEN PHOSPHORYLASE) and in plants (STARCH PHOSPHORYLASE).Myosin Heavy Chains: The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.Myosins: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.DNA Polymerase III: A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms but may be present in higher organisms. Use also for a more complex form of DNA polymerase III designated as DNA polymerase III* or pol III* which is 15 times more active biologically than DNA polymerase I in the synthesis of DNA. This polymerase has both 3'-5' and 5'-3' exonuclease activities, is inhibited by sulfhydryl reagents, and has the same template-primer dependence as pol II. EC 2.7.7.7.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Butirosin Sulfate: A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Schizophyllum: A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.ChitinaseLuminescent Measurements: Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.Nitrophenylgalactosides: Includes ortho-, meta-, and para-nitrophenylgalactosides.Lac Operon: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Photography: Method of making images on a sensitized surface by exposure to light or other radiant energy.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Monosaccharides: Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.

Fecal coliform elevated-temperature test: a physiological basis. (1/7076)

The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.5 degrees C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5 degrees C. A temperature increase from 35 to 44.5 degrees C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform beta-galactosidase activity coupled with thermal inactivation of this enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.  (+info)

The effects of digestive enzymes on characteristics of placental insulin receptor. Comparison of particulate and soluble receptor preparations. (2/7076)

The role of the surrounding membrane structure on the binding characteristics of the insulin receptor was studied by using several digestive enzymes. The effects observed with particulate membrane preparations are compared with those from soluble receptor preparations. beta-Galactosidase and neuraminidase had no effect on insulin binding to either particulate or soluble receptors from human placentae. Exposure to 2 units of phospholipase C/ml increased insulin binding to particulate membranes, but was without effect on the soluble receptor preparation. The increase in binding to particulate membranes was shown to be due to an increase in apparent receptor number. After 5 min exposure to 500 microgram of trypsin/ml there was an increase in insulin binding to the particulate membrane fraction, owing to an increase in receptor affinity. After 15 min exposure to this amount of trypsin, binding decreased, owing to a progressive decrease in receptor availability. In contrast, this concentration of trypsin had no effect on the solubilized receptor preparation. Because of the differential effects of phospholipase C and trypsin on the particulate compared with the solubilized receptor preparations, it is concluded that the effects of these enzymes were due to an effect on the surrounding membrane structure. Changes in receptor configuration due to alterations within the adjoining membrane provide a potential mechanism for mediating short-term alterations in receptor function.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (3/7076)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (4/7076)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Pathogenicity island 2 mutants of Salmonella typhimurium are efficient carriers for heterologous antigens and enable modulation of immune responses. (5/7076)

The potential use as vaccine delivery system of Salmonella typhimurium strains harboring defined mutations in the sseC (HH104) and sseD (MvP101) genes, which encode putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2, was evaluated and compared with that of the well-characterized aroA mutant strain SL7207 by using beta-galactosidase (beta-Gal) as a model antigen. When orally administered to immune-competent or gamma interferon-deficient (IFN-gamma-/-) BALB/c mice, both mutants were found to be highly attenuated (50% lethal dose, >10(9) bacteria). Both strains were also able to efficiently colonize and persist in Peyer's patches. Immunization with HH104 and MvP101 triggered beta-Gal-specific serum and mucosal antibody responses equivalent to or stronger than those observed in SL7207-immunized mice. Although immunoglobulin G2 (IgG2) serum antibodies were dominant in all groups, IgG1 was also significantly increased in mice vaccinated with MvP101 and SL7207. Comparable beta-Gal-specific IgA and IgG antibodies were detected in intestinal lavages from mice immunized with the different strains. Antigen-specific CD4(+) T-helper cells were generated after vaccination with all vaccine prototypes; however, responses were significantly more efficient when HH104 and MvP101 were used (P < 0.05). Significantly higher levels of IFN-gamma were produced by restimulated spleen cells from mice immunized with HH104 than from those vaccinated with the MvP101 or SL7207 derivatives (P +info)

Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes. (6/7076)

BACKGROUND: In congestive heart failure, high systemic levels of the hormone arginine vasopressin (AVP) result in vasoconstriction and reduced cardiac contractility. These effects are mediated by the V1 vasopressin receptor (V1R) coupled to phospholipase C beta-isoforms. The V2 vasopressin receptor (V2R), which promotes activation of the Gs/adenylyl cyclase system, is physiologically expressed in the kidney but not in the myocardium. Expression of a recombinant V2R (rV2R) in the myocardium could result in a positive inotropic effect via the endogenous high concentrations of AVP in heart failure. METHODS AND RESULTS: A recombinant adenovirus encoding the human V2R (Ad-V2R) was tested for its ability to modulate the cardiac Gs/adenylyl cyclase system and to potentiate contractile force in rat ventricular cardiomyocytes and in H9c2 cardiomyoblasts. Ad-V2R infection resulted in a virus concentration-dependent expression of the transgene and led to a marked increase in cAMP formation in rV2R-expressing cardiomyocytes after exposure to AVP. Single-cell shortening measurements showed a significant agonist-induced contraction amplitude enhancement, which was blocked by the V2R antagonist, SR 121463A. Pretreatment of Ad-V2R-infected cardiomyocytes with AVP led to desensitization of the rV2R after short-term agonist exposure but did not lead to further loss of receptor function or density after long-term agonist incubation, thus demonstrating resistance of the rV2R to downregulation. CONCLUSIONS: Adenoviral gene transfer of the V2R in cardiomyocytes can modulate the endogenous adenylyl cyclase-signal transduction cascade and can potentiate contraction amplitude in cardiomyocytes. Heterologous expression of cAMP-forming receptors in the myocardium could lead to novel strategies in congestive heart failure by bypassing the desensitized beta-adrenergic receptor signaling.  (+info)

Multiple cis-acting regulatory regions are required for restricted spatio-temporal Hoxa5 gene expression. (7/7076)

Genetic analyses have revealed the essential role of the murine Hoxa5 gene for the correct specification of the cervical and upper thoracic region of the skeleton, and for the normal organogenesis and function of the respiratory tract, both structures expressing Hoxa5 during embryogenesis. To understand how the expression domains of the Hoxa5 gene are established during development, we have analyzed the cis-acting control regions mediating Hoxa5 gene expression using a transgenic approach. Four transcripts are derived from the Hoxa5 locus. The shortest and most abundant one displays a specific spatio-temporal profile of expression at earlier stages and in more anterior structures along the embryonic axis than the larger forms. We established that an 11.1 kilobase pair (kb) genomic fragment, extending from position -3.8 kb to +7.3 kb relative to Hoxa5 transcription initiation site, was sufficient to reproduce the temporal expression and substantially reconstitute the spatial pattern of the major Hoxa5 transcript. By deletion analyses, we identified a 2.1 kb fragment located downstream of the Hoxa5 gene that possesses mesodermal enhancer activity. Overall, the findings demonstrate that cis-acting regulatory elements essential for the correct expression of the major Hoxa5 transcript are located both upstream and downstream of the Hoxa5 coding sequences.  (+info)

Ectopic expression of the transforming growth factor beta type II receptor disrupts mesoderm organisation during mouse gastrulation. (8/7076)

Transforming growth factor beta (TGFbeta) regulates the cell cycle and extracellular matrix (ECM) deposition of many cells in vitro. We have analysed chimaeric mouse embryos generated from embryonic stem cells with abnormal receptor expression to study the effect of TGFbeta on these processes in vivo and the consequences for normal development. The binding receptor for TGFbeta, TbetaRII, is first detected in the embryo proper around day 8.5 in the heart. Ectopic expression of TbetaRII from the blastocyst stage onward resulted in an embryonic lethal around 9.5 dpc. Analysis of earlier stages revealed that the primitive streak of TbetaRII chimaeras failed to elongate. Furthermore, although cells passed through the streak and initially formed mesoderm, they tended to accumulate within the streak. These defects temporally and spatially paralleled the expression of the TGFbeta type I receptor, which is first expressed in the node and primitive streak. We present evidence that classical TGFbeta-induced growth inhibition was probably the cause of insufficient mesoderm being available for paraxial and axial structures. The results demonstrate that (1) TGFbeta mRNA and protein detected previously in early postimplantation embryos is present as a biologically active ligand; and (2) assuming that ectopic expression of TbetaRII results in no other changes in ES cells, the absence of TbetaRII is the principle reason why the embryo proper is unresponsive to TGFbeta ligand until after gastrulation.  (+info)

*Beta-galactosidase

A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... proposed a new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) ... "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111/j.1474- ... β-galactosidase synthesis stops when glucose levels are sufficient. Beta-galactosidase has many homologues based on similar ...

*Endo-beta-galactosidase

... may refer to: Blood-group-substance endo-1,4-beta-galactosidase Keratan-sulfate endo-1,4-beta- ...

*Senescence-associated beta-galactosidase

"Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111/j.1474- ... Senescence-associated beta-galactosidase (SA-β-gal or SABG) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of ... Senescence-associated beta-galactosidase, along with p16Ink4A, is regarded to be a biomarker of cellular senescence. Its ... following the observation that when beta-galactosidase assays were carried out at pH 6.0, only cells in senescence state ...

*6-phospho-beta-galactosidase

... beta-D-phosphogalactoside galactohydrolase, phospho-beta-D-galactosidase, and 6-phospho-beta-D-galactosidase. This enzyme ... In enzymology, a 6-phospho-beta-galactosidase (EC 3.2.1.85) is an enzyme that catalyzes the chemical reaction a 6-phospho-beta- ... Hengstenberg W, Penberthy WK, Morse ML (1970). "Purification of the staphylococcal 6-phospho-beta-D-- galactosidase". Eur. J. ... Other names in common use include phospho-beta-galactosidase, ... The systematic name of this enzyme class is 6-phospho-beta-D- ...

*Galactan 1,3-beta-galactosidase

3-beta-galactosidase (EC 3.2.1.145, galactan (1->3)-beta-D-galactosidase) is an enzyme with systematic name galactan 3-beta-D- ... Galactan 1,3-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... galactosidase. This enzyme catalyses the following chemical reaction Hydrolysis of terminal, non-reducing beta-D-galactose ... residues in (1->3)-beta-D-galactopyranans This enzyme removes not only free galactose, but also 6-glycosylated residues. ...

*Galactan endo-1,6-beta-galactosidase

... (EC 3.2.1.164, endo-1,6-beta-galactanase) is an enzyme with systematic name endo-beta-(1-> ... Galactan endo-1,6-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ... beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products The enzyme specifically hydrolyses 1,6- ... This enzyme catalyses the following chemical reaction Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan ...

*Arabinogalactan endo-1,4-beta-galactosidase

Arabinogalactan endo-beta-1,4-galactanase (EC 3.2.1.89, endo-1,4-beta-galactanase, galactanase, arabinogalactanase, ganB (gene ... Arabinogalactan endo-beta-1,4-galactanase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ... This enzyme catalyses the following chemical reaction The enzyme specifically hydrolyses (1->4)-beta-D-galactosidic linkages in ... and biochemical evidence that some glycoside hydrolase family 42 β-galactosidases are arabinogalactan type I oligomer ...

*Keratan-sulfate endo-1,4-beta-galactosidase

... (EC 3.2.1.103, endo-beta-galactosidase, keratan sulfate endogalactosidase, ... Keratan-sulfate endo-1,4-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular ... keratanase, keratan-sulfate 1,4-beta-D-galactanohydrolase) is an enzyme with systematic name keratan-sulfate 4-beta-D- ... This enzyme catalyses the following chemical reaction Endohydrolysis of (1->4)-beta-D-galactosidic linkages in keratan sulfate ...

*Blood-group-substance endo-1,4-beta-galactosidase

... (EC 3.2.1.102, endo-beta-galactosidase, blood-group-substance 1,4-beta-D- ... Blood-group-substance endo-1,4-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) ... This enzyme catalyses the following chemical reaction Endohydrolysis of (1->4)-beta-D-galactosidic linkages in blood group A ... Fukuda, M.N.; Matsumara, G. (1976). "Endo-β-galactosidase of Escherichia freundii. Purification and endoglycosidic action on ...

*GM1 gangliosidoses

Autosomal recessive disorder; beta-galactosidase deficiency; neuronal storage of GM1 ganglioside and visceral storage of ... The GM1 gangliosidoses (or GM1 gangliosidosis) are caused by a deficiency of beta-galactosidase, with resulting abnormal ... Prenatal diagnosis is possible by measurement of Acid Beta Galactosidase in cultured amniotic cells. Bley, Annette E.; ... resulting from marked deficiency of Acid Beta Galactosidase. GM1 has three forms: early infantile, late infantile, and adult. ...

*Glycoside hydrolase family 35

... beta-galactosidase (EC 3.2.1.23). Mammalian beta-galactosidase is a lysosomal enzyme (gene GLB1) which cleaves the terminal ...

*Glycoside hydrolase family 1

... beta-galactosidase (EC 3.2.1.23); 6-phospho-beta-galactosidase (EC 3.2.1.85); 6-phospho-beta-glucosidase (EC 3.2.1.86); lactase ... 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT; LCTL; GH1 in CAZypedia Henrissat B, Callebaut I, Mornon JP ... Glycoside hydrolase family 1 CAZY GH_1 comprises enzymes with a number of known activities; beta-glucosidase (EC 3.2.1.21); ... hydrolase (EC 3.2.1.62), lactase (EC 3.2.1.108); beta-mannosidase (EC 3.2.1.25); myrosinase (EC 3.2.1.147). ...

*Glycoside hydrolase family 16

... endo-beta-1,3-glucanase (EC 3.2.1.39); endo-beta-1,3-1,4-glucanase (EC 3.2.1.6); endo-beta-galactosidase (EC 3.2.1.103). ...

*Blue-white screen

Langley, K. E.; Villarejo, M. R.; Fowler, A. V.; Zamenhof, P. J.; Zabin, I. (1975). "Molecular basis of beta-galactosidase ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ...

*Marker gene

The bacterial lacZ gene encodes a beta-galactosidase enzyme. When media containing certain galactosides (e.g. X-gal) is added, ...

*Cathepsin A

1991). "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases". ... 1994). "Normal serum beta-galactosidase in juvenile GM1 gangliosidosis". Pediatr. Neurol. 10 (4): 317-9. doi:10.1016/0887-8994( ... Chakraborty S, Rafi MA, Wenger DA (1994). "Mutations in the lysosomal beta-galactosidase gene that cause the adult form of GM1 ... 1991). "Combined deficiency of beta-galactosidase and neuraminidase: natural history of the disease in the first 18 years of an ...

*Glycoside hydrolase family 2

... beta-galactosidase (EC 3.2.1.23); beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a ... The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... Matthews BW, Jacobson RH, Zhang XJ, DuBose RF (1994). "Three-dimensional structure of beta-galactosidase from E. coli". Nature ... beta-galactosidase from Escherichia coli". J. Biol. Chem. 267 (16): 11126-11130. PMID 1350782. ...

*Galactosialidosis

Neuraminidase deficiency with beta-galactosidase deficiency at NIH's Office of Rare Diseases Hide & Seek Foundation for ... in 1979 by measuring beta-galactosidase and neuraminidase activities in cultured amniotic fluid cells. Koike K, Hamaguchi T, ... This in turn leads to a secondary deficiency of beta-galactosidase (GLB1) and neuraminidase 1 (NEU1).[citation needed]There are ... "Prenatal diagnosis of sialidosis with combined neuraminidase and beta-galactosidase deficiency". Clinical Genetics. 16 (1): 60- ...

*Penicillium multicolor

ATCC Flood, M. T.; Kondo, M (2004). "Toxicity evaluation of a beta-galactosidase preparation produced by Penicillium multicolor ... "Isolation and characterization of a beta-primeverosidase-like enzyme from Penicillium multicolor". Bioscience, Biotechnology, ...

*NEU1

In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter ... Activation, stabilization and association with beta-galactosidase and its protective protein". Eur. J. Biochem. 149 (2): 315-21 ... "Association of N-acetylgalactosamine-6-sulfate sulfatase with the multienzyme lysosomal complex of beta-galactosidase, ...

*Lactase

... beta-galactosidase". Biochemistry. 40 (49): 14781-94. doi:10.1021/bi011727i. PMID 11732897. Fernandez P, Cañada FJ, Jiménez- ... Lactase (also known as lactase-phlorizin hydrolase, or LPH), a part of the β-galactosidase family of enzymes, is a glycoside ... The Lactase Protein E. coli β-galactosidase: PDB: 1JYY​ Gene Ontology for Lactase Making of the Fittest: Got Lactase? The Co- ... Hermida C, Corrales G, Cañada FJ, Aragón JJ, Fernández-Mayoralas A (Jul 2007). "Optimizing the enzymatic synthesis of beta-D- ...

*Eastern blot

Olson and Eglen; Eglen, RM (2007). "beta Galactosidase complementation: A cell-based luminescent assay platform for drug ...

*Protein-fragment complementation assay

... beta-galactosidase) Luciferase, including ReBiL (recombinase enhanced bimolecular luciferase) and the commercial products ... "Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation". Proceedings of the ... The following proteins have been used in split protein PCAs: Beta-lactamase Dihydrofolate reductase (DHFR) Focal adhesion ... "Bacterial beta-lactamase fragmentation complementation strategy can be used as a method for identifying interacting protein ...

*François Jacob

Ullmann, A.; Jacob, F.; Monod, J. (1968). "On the subunit structure of wild-type versus complemented beta-galactosidase of ... "The role of the inducible alleles and the constitutive alleles in the synthesis of beta-galactosidase in zygotes of Escherichia ... of a peptide fraction of Escherichia coli beta-galactosidase". Journal of Molecular Biology. 12 (3): 918-923. doi:10.1016/S0022 ... by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural ...

*Affibody molecule

"Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol". J. Immunol. ...

*Potassium ferrocyanide

... is used in a mixture with potassium ferricyanide and phosphate buffered solution to provide a buffer for beta-galactosidase, ... which is used to cleave X-Gal, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal ...
Beta Galactosidase | Learn more about Beta Galactosidase | Meaning of Beta Galactosidase | Description of Beta Galactosidase | Details of Beta Galactosidase | Article on Beta Galactosidase | Essay on Beta Galactosidase | Definition of Beta Galactosidase | Infospaze
microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Quantitation of β-galactosidase activity. In yeast cells, co-transformed with pGADT7 (AD) and pGBKT7 (BD) constructs as indicated, β-galactosidase activity wa
Buy our Recombinant |em|E. coli |/em| beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols…
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
|strong|Rabbit anti beta-galactosidase polyclonal antibody|/strong| detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccha…
BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.) ...
METHODS AND RESULTS The method involves the harvest of autologous venous-derived endothelial cells, the efficient genetic modification of the cells through the use of recombinant retroviruses, and the subsequent implantation of the genetically modified cells on the surface of balloon-denuded arterial segments. With a rabbit model, freshly isolated endothelial cells were transduced with a recombinant retrovirus encoding the bacterial enzyme beta-galactosidase. The autologous transduced cells were then implanted on the surface of balloon-denuded ileofemoral arterial segments at different cell densities; after 1 to 14 days, the animals were killed, and the vessel segments were examined. Cells expressing the bacterial gene product, as determined by in situ staining for beta-galactosidase, were found to be present on the surface of 28 of the 32 arteries seeded with genetically modified cells. Vessels examined at 4 to 7 days after seeding displayed 40% to 90% coverage with transduced cells, even when ...
Beta-galactosidase, 0.1 ml. This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates.
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic units (monosaccharides). - Stock Image C015/1970
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
The cae8 promoter is sensitive to the amino acid L-asparagine.The figure shows measured β-galactosidase activities from wild-type BW25113 cells, carrying the c
Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C
C-terminal BetaGal reporter tag yeast expression plasmid. This vector allows the creation of reporter fusion proteins with no protease cleavage tag.
lacZ. No transcription. Structural genes lacZ. lacY. lac A. No transcription. Presence of lactose. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor). Allolactose. 12 When lactose is present, some of it is converted into allolactose,.... RNA polymerase lacO operator. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor) ...
Misc.Comments : The SK designation indicates the polylinker is oriented such that beta-galactosidase (lacZ) transcription proceeds through the SacI site first and the KpnI site last. pBluescript SK(+) carries an F1 origin of replication, oriented such that transcription proceeds in the same direction as beta-galactosidase (lacZ) transcription ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
ATCC ® 87590™ Designation: YEplac112 TypeStrain=False Application: YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
BioAssay record AID 404864 submitted by ChEMBL: Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene assay relative to control.
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase ...
Substâncias complexas são convertidas, pela ação de enzimas, em moléculas solúveis, durante o processo de germinação, as quais são translocadas para a plântula em crescimento, servindo como fonte de energia ou estrutura física. Com o objetivo de quantificar a atividade enzimática da -galactosidase e a mobilização de mono e oligossacarídeos durante o período de germinação, foi conduzido este estudo utilizando-se sementes de jacarandá-da-bahia. As sementes foram mantidas em germinador a 25oC sob luz contínua, sendo avaliada a protrusão da radícula, pelo período de 10 dias. Foram quantificados os teores de mono e de oligossacarídeos, assim como a atividade específica da enzima -galactosidase nos tempos zero, um, três e cinco dias. Houve mobilização das reservas de glicose e manose nos cotilédones e xilose neste e no embrião nos três primeiros dias de germinação. A ramnose teve os teores aumentados nos cotilédones e no eixo embrionário. A rafinose foi o ...
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
You might also use the Kyoto Encyclopedia of Genes and Genomes, which has a lot of information conveniently summarized for many proteins; for example, the entry for the E. coli beta-galactosidase gene is here. Note that the entries for orthologies, pathways, motifs, cross-references to other databases, structures, etc. are all clickable.. ...
It was found that Concanavalin A (Con A) accelerates the rates of hydrolysis of E. coli beta-galactosidase and yeast invertase by binding to the product (glucose) formed in the reaction. The effect of Con A can be made ...
BioAssay record AID 142597 submitted by ChEMBL: Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in NIH 3T3 cells. Maximal effect normalized to the effect of carbachol (%CCH)..
The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert presence of insert results into insertional inactivation of Beta galactosidase and recombinant colonies do not produce any colour these are identified as Recombinant Colony
Cellular senescence is a fundamental cell fate playing significant and complex roles during tumorigenesis and natural aging process. However, the molecular determinants distinguishing senescence from other temporary and permanent cell-cycle arrest states such as quiescence and post-mitotic state and the specified mechanisms underlying cell-fate decisions towards senescence versus cell death in response to cellular stress stimuli remain less understood. In our studies, we aimed to employ multi-omics approaches to deepen our understanding of cellular senescence, in particular, regarding the specific molecular determinants distinguishing cellular senescence from other non-dividing cell fates. Notably, one of the most prominent features of cellular senescence differing from other non-dividing cell fates is the increased expression of senescence-associated beta-galactosidase. Because 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG) is known as the substrate catalyzed by ...
A Q: for transfection gurus. We are measuring promoter activity using luciferase gene as a main reporter, fused to the putative promoter fragments, and CMV/beta-galactosidase gene as a second, normalizer activity. Both are introduced by lipofection into an aortic cell line. Luciferase activity is measured using a Promega kit, and the b-gal is measured via a chemiluminescence based kit from Tropix (GalactoLight). Before measuring the latter activity we heat-inactivate the endogenous activity. What we get is that for a given construct the luciferase activity in a 3X transfection series is relatively constant ( within a factor of 1.5), but the respective set of b-gal activities is variable within a factor of 3, which ruins the experiment. Any ideas? Has anyone used GalactoLight with success? -- Alexander Kraev, PhD Biochemie III, ETHZ Zurich Phone 41-1-632-31-47 Fax 41-1-632-12-13 e-mail kraev at bc.biol.ethz.ch ...
Using a fungus two-hybrid system, we isolated a book human centrosomal protein, CPAP (centrosomal P4. utilized to display screen for protein that connect to 4.1R-135. The top domains (HD; residues 1 to 209) of 4.1R-135 (4.1R-HD) was fused towards the GAL4 DNA-binding domains (GAL4-DB) in vector pAS2-1 (Clontech). This create was used as bait to display a Z-VAD-FMK inhibition human being lymphocyte cDNA library fused to a GAL4 activation website (GAL4-AD) in the pACT2 vector (Clontech). The two types of plasmids were then cotransformed into Y190, and the transformants were selected on SD minimal medium as previously explained (40). Positive colonies were further tested for -galactosidase activity using a colony-lift assay and liquid assay as explained by the manufacturer (Clontech). To thin down the head website region of 4.1R (4.1R-HD) that binds to CPAP, constructs containing numerous portions of 4.1R-HD were fused to GAL4-DB of the pAS2-1 vector (Fig. ?(Fig.1A).1A). The C terminus of CPAP ...
Dimerization data (column 1) represent relative binding to GST-TBP(181C) as determined in Figure 1E and are an average of six sets of data. See Figure 5 for standard errors. β-galactosidase activity (columns 3-6) is relative to wild-type test TBP in column 3 (1.0 = 3 Miller units using the high-sensitivity substrate CPRG) and represents an average of at least three independent determinations. All data were obtained in the linear range of the assay. The null allele expresses only the first 81 amino acids of TBP. "Test" HA-tagged TBP levels were determined by quantitative Western blotting with TBP antibodies as described in Figure 5B. TBP levels are relative to the endogenous (untagged) TBP (1 = 17,000 ± 2,000 molecules per cell). The standard error for all data is presented graphically in Figure 5E. Expression of the TBPEBmutants did not cause a decrease in the expression of TAFII145 (data not shown). "Low" in columns 3 and 7 indicates that the test TBP was driven by the TBP promoter; "high" in ...
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The results of this study are consistent with the following conclusions. First, unconjugated β-galactosidase is rapidly cleared from blood in vivo (Table 2), owing to rapid uptake of the unconjugated enzyme by liver and spleen (Figs. 2 and 3). Second, once inside cells, β-galactosidase is rapidly degraded in vivo such that 99% of the organ enzyme activity is lost at 4 h after an intravenous injection (Table 1). Third, the 116-kDa β-galactosidase (Fig. 1B) can be conjugated to the 8D3 TfRmAb without loss of enzyme activity (Fig. 1C). Fourth, there is minimal brain uptake of the unconjugated β-galactosidase, but there is a 10-fold increase in brain uptake of enzyme following conjugation to the 8D3 TfRmAb (Table 1; Figs. 2 and 3).. The β-galactosidase is rapidly removed from the blood due to the avid uptake of the enzyme by liver and spleen (Figs. 2 and 3), which confirms the earlier observation of Onodera et al. (1983). The blood concentration of the β-galactosidase-TfRmAb is 5- to 10-fold ...
ATCC ® 77267™ Designation: pUN65 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Page contains details about β-galactosidase/ZIF-8 MOF coating . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Expresses the head domain of Nod fused to the coiled-coil domain of KHC and beta-galactosidase under the control of UAS; the fusion protein accumulates at the minus ends of microtubules, N.G ...
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols. ...
reacts with native and denatured-reduced E. coli β-galactosidase(116 kD); may be usedfor detection of β-galactosidase expressed by E. colilacZ gene encoded in many cloned gene sequences,and serves as an indicator for fusion proteins encodedby an inserted DNA ...
In article ,leach-051093092638 at med-pharm5.bu.edu,, leach at mbcrr.harvard.edu (Martin Leach) writes: , In article ,andres.37.749754284 at calvin.jci.tju.edu,, , andres at calvin.jci.tju.edu (Andres Ferber) wrote: , ,, Does anybody know of a reference for staining mammalian cells transfected ,, with betagalactosidase constructs? ,, I am interested in staining the cells alive by adding Xgal to the culture ,, media without fixing the cells. Is it possible? Any suggestions or comments? ,, Andres Ferber , I have an old protocol that works well, instead of posting phone PROMEGA at , 1-800-356-9526 and they can fax you their protocol in their b-gal assay , booklet. , Martin Leach Hi, Ive done X-gal staining on fixed mamalian cells expressing lacZ, but can you really do it on live cells without fixing???? ^^^^^^^^^^ -- Cheers, Martin NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ NN NN NN Christchurch School of Medicine ZZZ ...
S fimbrial adhesins (Sfa) enable pathogenic Escherichia coli strains to bind to sialic acid-containing eucaryotic receptor molecules. In order to determine the influence of culture conditions on the expression of the sfa determinant in a wild-type strain, we fused the gene lacZ, coding for the enzyme beta-galactosidase, to the sfaA gene, responsible for the major protein subunit of S fimbriae. By using a plasmid which carries an R6K origin, the sfaA-lac hybrid construct was site-specifically integrated into the chromosome of the uropathogenic E. coli strain 536WT. The expression of lacZ, which was under the control of the sfa wild-type promoters, was now equivalent to the sfa expression of strain 536WT. With the help of this particular wild-type construct, it was demonstrated that the sfa determinant is better expressed on solid media than in liquid broth. The growth rate had a strong influence on Sfa expression under aerobic but not under anaerobic conditions. Production of Sfa was further ...
There are no specific protocols for Recombinant Treponema Membrane Protein A (Beta-Galactosidase) (ab54101). Please download our general protocols booklet
1. A method is described for following continuously the action of beta-galactosidase on 4-methylumbelliferyl beta-D-galactoside at pH 4.5, in which 4-methylumbelliferone production is measured at fluorescence excitation and emission wavelengths of 324 and 444nm respectively. 2. Initial-rate studies show that the presence of salt activates beta-galactosidase up to 100 mM, but is inhibitory above that concentration. The enzyme is very unstable at 37 degrees C and low ionic strength, but stability increases with ionic strength. 3. The stability of the enzyme at 37 degrees C decreases markedly with rising pH in the range 5.9-8.0. 4. Gel-filtration patterns demonstrate that there is a marked tendency to polymerization with increasing ionic strength. The gel-filtration pattern shows decreasing amounts of dimer with increasing pH. 5. The correlation between activity, stability and molecular form of beta-galactosidase is discussed. It is suggested that the dimeric form of the enzyme is the most stable ...
DNA constructs and protein interaction assays. All constructs were generated by subcloning PCR amplification products into appropriate vectors, and each construct was verified by automated DNA sequencing. cDNA fragments encoding the C terminus of the D1 (residues 365-446), D2 (residues 428-443), D3 (residues 385-400), D4 (residues 370-387), and D5 (residues 360-477) receptors were ligated into the yeast GAL4 DNA-binding domain expression vector pAS2-1 (Clontech, Palo Alto, CA). For the D2 receptor screen, the D2-pAS2-1 bait plasmid and the human brain cDNA library in the GAL4 activation domain vector pACT2 (Clontech) were simultaneously cotransformed into the yeast strain MaV103 as previously described (Lin et al., 2001). Positive clones were identified by growth on Leu−/Trp−/His−/Ura−selection plates. Protein interaction was assayed for by β-galactosidase activity via the nitrocellulose filter lift method (Lin et al., 2001).. To identify the sites of interaction between D2 or D3 ...
Neste trabalho foi desenvolvido um novo bioprocesso para a síntese de lactosacarose, um candidato a prebiótico. A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. No processo de imobilização, os resultados indicam que a melhor razão entre a concentração de enzima e de suporte foi de 200 mg.g-1. A estabilidade térmica da enzima imobilizada foi determinada e comparada com a estabilidade térmica da enzima livre em temperaturas de 50, 60 e 70 °C e para esta última foi verificada a estabilidade na presença e ausência de substrato. A imobilização aumentou de 10 a 260 vezes a estabilidade térmica da enzima, sendo este efeito inversamente relacionado com a temperatura. A otimização das condições de produção indica, para a β-galactosidase imobilizada e livre, que a melhor condição de produção de lactosacarose e de ...
Myc-DDK-tagged ORF clone of Homo sapiens UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2) as transfection-ready DNA - 10 µg - OriGene - cdna clones
Page contains details about β-galactosidase expressing plasmid encapsulating liposomes . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
The function of lactic acid microbes in gastrointestinal microecology has actually been the subject of substantial investigate. It is broadly thought that these microbes avert The expansion of putrefactive microorganisms accountable for sick wellbeing by competitive inhibition, the era of the non-conducive acidic ecosystem and/or from the creation of bacteriocins. Their metabolites may contain B team vitamins. Their proteolytic, lipolytic and beta-galactosidase routines advertise the digestibility and assimilation of ingested nutrients, therefore rendering them worthwhile in convalescent/ geriatric nourishment ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Theoretically unlimited TDP is possibly one of the silliest things Ive ever read. It would require theoretically unlimited power for starters,...
To determine whether the N-terminal hydrophobic sequence is also responsible for retention, the N-terminal 29 amino acids of cytochrome P450 2C1, with and without an additional 29 amino acids containing an N-glycosylation site, were fused to a soluble cytoplasmic protein, Escherichia coli $\beta$-galactosidase, or to a secreted protein, Escherichia coli alkaline phosphatase, and the hybrid proteins were expressed in COS1 cells and cellular localization was determined by subcellular fractionation, immunolocalization and the glycosylation state of the proteins. Both the $\beta$-galactosidase and alkaline phosphatase hybrid proteins were retained in the endoplasmic reticulum establishing that a specific sequence or property in the N-terminal 29 amino acids is responsible for ER retention. To further examine the possibility that retention of proteins with a large cytoplasmic domain is the default pathway, the cellular distributions of a series of P450 2C1 and EGFR chimeric proteins were examined. ...
Premature senescence of human diploid fibroblasts (HDFs) can be induced by exposures to a variety of oxidative stress and DNA damaging agents. In this study we developed a robust model of UVB-induced premature senescence of skin HDFs. After a series of 10 subcytotoxic (non-proapoptotic) exposures to UVB at 250 mJ/cm2, the so-called biomarkers of senescence were markedly expressed: growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene overexpression, deletion in mitochondrial DNA. A set of 44 stress- and senescence-associated genes were found to be differentially expressed in this model, among which clusterin/apolipoprotein J (apo J) and transforming growth factor-β1 (TGF-β1). Transfection of apo J cDNA provided protection against premature senescence-inducing doses of UVB and other stressful agents. Neutralizing antibodies against TGF-β1 or its receptor II (TβRII) sharply attenuated the senescence-associated features, suggesting a role for TGF-β1 in ...
A technique for the transfer of endothelial cells and expression of recombinant genes in vivo could allow the introduction of proteins of therapeutic value in the management of cardiovascular diseases. Porcine endothelial cells expressing recombinant beta-galactosidase from a murine amphotropic retroviral vector were introduced with a catheter into denuded iliofemoral arteries of syngeneic animals. Arterial segments explanted 2 to 4 weeks later contained endothelial cells expressing beta-galactosidase, an indication that they were successfully implanted on the vessel wall. ...
The structure of DNA was discovered in 1953, and its role as the physical repository of genes illuminated in the following decades. How information encoded as nucleic acid is expressed as proteins is well-known. The magic of protein synthesis is that DNA is only transcribed into mRNA when the protein it codes for is needed. E.G.: The bacterium Escherichia coli freely metabolizes glucose, but if glucose is lacking, and lactose is present, it can metabolize it by producing an enzyme, beta-galactosidase, that breaks lactose down in to glucose and galactose. But how does it know when to make beta-galactosidase? The work of François Jacob and Jacques Monod established the answer (Nobel Prize in 1965): Adjacent to the gene for beta-galactosidase is a small gene to which a protein, the lac repressor binds. This blocks RNA polymerase from unzipping the DNA and making mRNA. But, lactose, itself, binds to the lac repressor causing it to fall off of the DNA strand and allowing RNA polymerase to do its ...
40. Appellants argue that after the publication of Polisky, successful synthesis of protein was still uncertain. They belittle the predictive value of the observation that expression of the transcribed RNA in Polisky produced beta-galactosidase with a greater than normal molecular weight, arguing that since ribosomal RNA is not normally translated, the polypeptide chains that were added to the end of the beta-galactosidase were "junk" or "nonsense" proteins. This characterization ignores the clear implications of the reported observations. The Polisky study directly proved that a readthrough transcript messenger RNA had been produced. The preliminary observation showed that this messenger RNA was read and used for successful translation. It was well known in the art that ribosomal RNA was made of the same nucleotides as messenger RNA, that any sequence of nucleotides could be read in groups of three as codons, and that reading these codons should specify a polypeptide chain that would elongate ...
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-
This exercise was designed to provide an exciting introduction to specific gene structure and function. In the experiment, students are given two plasmids (A and B) which are identified in the instructors guide. One plasmid (A) has a functional gene for the enzyme ß-galactosidase. The ß-galactosidase gene in the other plasmid (B) is inactive because it contains a segment of foreign DNA. In the first part of the exercise, students analyze restriction digests of both plasmids in order to determine which plasmid should have a functional ß-galactosidase gene. In the second part of the exercise, the plasmids are introduced into E. coli by transformation and the color of the resulting colonies (blue or white) is then used to assess the functional status of the ß-galactosidase gene. This exercise can be completed within a single 3-hour laboratory session or two 2-hour laboratory periods.. ...
The position of lactic acid microorganisms in gastrointestinal microecology has been the subject of intensive study. It is widely thought that these bacteria stop The expansion of putrefactive microorganisms liable for sick well being by aggressive inhibition, the era of the click here non-conducive acidic surroundings and/or with the creation of bacteriocins. Their metabolites may include things like B group vitamins. Their proteolytic, lipolytic and beta-galactosidase actions market the digestibility and assimilation of ingested nutrients, thereby rendering them valuable in convalescent/ geriatric nourishment ...
β-galactosidase (EC 3.2.1.23); exo-β-glucosaminidase (EC 3.2.1.165); exo-β-1,4-galactanase (EC 3.2.1.-); β-1,3-galactosidase (EC 3.2.1.- ...
LacZ PCR Problems - posted in PCR, RT-PCR and Real-Time PCR: Hi, I am new to the forum and I have been battling a PCR problem for months now. I have to genotype mice for the presence of the LacZ gene because the targeting vector used contained LacZ. I extract the DNA from tails using a DirectPCR reagent from Viagen with proteinase K. These DNA extracts are then subjected to PCR using the LacZ primers that the jackson laboratory uses. I inherited this project from someone else and I have ne...
B3gnt7 - B3gnt7 (untagged ORF) - Rat UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 (B3gnt7), (10 ug) available for purchase from OriGene - Your Gene Company.
Magenta-Gal is an X-Gal alternative that is used with IPTG to select successfully transformed cells. Magenta-Gal with IPTG is used in red-white screening.
Vascular smooth muscle cells contribute to the formation of atherosclerotic plaques by proliferating in response to vascular injury and releasing growth-promoting factors. Because their autocrine and paracrine effects are not fully understood, expression of such growth factor genes in specific cell types in vivo would help to determine their mechanism of action. We describe a method to transfer vascular smooth muscle cells expressing recombinant gene products to localized segments of the arterial wall. Vascular smooth muscle cells from the inbred Yucatan minipig were infected in vitro with an amphotropic, replication-defective retrovirus transducing the gene for Escherichia coli beta-galactosidase. Vascular smooth muscle cells expressing this recombinant gene were implanted, using a catheter, into denuded iliofemoral artery segments of pigs in vivo. These arteries subsequently demonstrated beta-galactosidase activity in cells of the intima and media. This method, which provides for the ...
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases. ...
Allcosmeticsource.com alpha galactosidase 2000u/g,5kg/bag,free shipping [EP170508006]- alpha galactosidase 2000u/g,1kg/bag,free shipping What is alpha galactosidase 2000u/g alpha galactosidase is an enzyme used to hydrolyze or break α-1, 6-glycosidic bonds into galactosyl oligosaccharides (α-galactosides), liberating simpler, more usable sugars and eliminating its anti-nutrient effect. Thus, it improves the utilization of the energy and proteins in feedstuff. Function of alpha galactosidase 2000u/g Hydrolyzing
The yeast ADR1 protein contains two zinc finger domains that are essential for its role in transcriptional activation of alcohol dehydrogenase (ADH2). These domains are thought to function as DNA-binding structures. An ADR1-beta-galactosidase fusion protein made in Escherichia coli and containing the finger domains of ADR1 binds in vitro in a zinc-dependent manner to DNA fragments containing the two ADH2 upstream activation sequences. The strongest binding is to upstream activation sequence 1, a 22-base-pair palindrome. ...
Gaucher Disease is the most prevalent lysosomal glycolipid storage disorder, and is caused by an acid beta galactosidase deficiency. Symptoms of Gaucher Disease, which range from mild to severe, are a result of the accumulation of a fatty substance (lipid) called glucocerebroside in bone marrow cells. The most common symptoms include an enlarged spleen, an enlarged liver, frequent nosebleeds, anemia, poor blood clotting, and a reduced blood platelet count.
Primary osteoblast cultures from calvaria of newborn, wild-type, and Lrp5−/− mice were established and mineralized in vitro as previously described (Ducy et al., 1999). Transfections were performed in 24-well plates (20,000 cells/well; Fugene6; Roche) in triplicate and repeated at least three times; cells were harvested 40 h after transfection. Empty vector was added to keep the total amount of DNA constant at 225 ng/well. The FOPtkluc reporter, containing mutated Lef1 binding sites, was used in control transfections. All luciferase values were corrected for β-galactosidase activity as a control for transfection efficiency. For Western blot analysis, primary osteoblast lysates were collected in physiological buffer with protease inhibitor cocktail (Roche), homogenized, fractionated into cytosolic and membrane fractions by ultracentrifugation at 100,000 g for 90 min, and separated by SDS-PAGE. The epitope tags were detected by the anti-FLAG M2 antibody (Sigma-Aldrich) and anti-HA antibody ...
We previously published a detailed survey of the spatio‐temporal expression of Runx3 during embryonic development (Levanon et al, 2001). Runx3 expression was examined at embryonic day (E) 10.5 and between E14.5 and E16.5, and compared to the expression pattern of Runx1. Immunohistochemistry (IHC) and knock‐in (KI) β‐galactosidase activity (LacZ staining) were used in parallel throughout this analysis to rigorously determine the expression patterns of the two TFs. Runx3 and Runx1 were readily detected in different compartments of the haematopoietic system and also in the dorsal root ganglia (DRG), epidermal appendages and developing skeletal elements (Levanon et al, 2001). However, regarding epithelia an interesting distinction was noted in the expression pattern of Runx1 and Runx3. While Runx1 was expressed in various epithelia including mucosa of the oesophagus and stomach, the salivary glands ducts and the olfactory and respiratory mucosa, Runx3 expression was undetectable in these ...
Given that we have these two excellent systems that together over the last 15 years have been used to express up to 80% of all reported recombinant genes, what else is still needed? Imagine setting up a laboratory working to express recombinant eukaryotic genes for biochemical and structural analysis. Which recombinant expression systems need to be implemented? Numerous important factors must be considered before expression of a recombinant gene is attempted. What is the mass of the polypeptide? Multi domain proteins are typically more difficult to produce than the alternative single domain deletion mutants. Does the protein contain any disulfide bonds? Proteins with disulfide bonds would most likely not be correctly folded in E. coli [11]. Are any PTMs required for protein folding, stability, or function, and what is the final destination of the protein--secreted, in the cytoplasm, or incorporated into the membrane?. Based on published studies, E. coli and P. pastoris enable the expression of ...
Twin boys with Fabrys disease and 6 affected relatives were described. Limb pains and retinal vessel tortuosity were present but no patient had angiokeratomata. One boy had a severe enteropathy with small and large bowel involvement which was investigated. Thin-layer chromatography showed that excesses of ceramide di- and trihexosides were excreted in the urine. Leucocyte α-galactosidase activity was measured: hemizygous males showed very low activity, while obligate and probable heterozygous females had values intermediate between those of the patients and the normal controls.. ...
Alpha galactosidase A Antibody 66121-1-Ig has been identified with IF, IHC, WB, ELISA. 66121-1-Ig detected 49 kDa band in HeLa cells with 1:500-1:2000 dilution...
Alpha galactosidase A Antibody 19877-1-AP has been identified with IF, WB, ELISA. 19877-1-AP detected 49 kDa band in HEK-293 cells with 1:500-1:3000 dilution...
MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides6-phospho-beta-galactosidase (TIGR01233; EC 3.2.1.85; HMM-score: 215.8) ...
beta-Gal antibody [N2C3] (galactosidase, beta 1) for IHC-P, WB. Anti-beta-Gal pAb (GTX104360) is tested in Human, Rat samples. 100% Ab-Assurance.
First-generation, E1-deleted adenoviral vectors (E1-AV) can transduce the vascular endothelium with high efficiency, but their use is limited by the resulting acute endothelial injury and the long-term development of intimal hyperplasia. To reduce the impact of viral proteins on the gene-modified cells, a second-generation adenoviral vector with an additional pair of deletions in the E4 region was developed. To determine whether this E1/E4-AV vector would be useful for vascular gene transfer, we directly compared the efficiency of gene transfer to uninjured rabbit carotid arteries using either an E1/E4-AV or an E1-AV vector encoding beta-galactosidase. Both vectors efficiently transduced vascular endothelium; however, the E1/E4-AV vector gene-modified vessels showed higher beta-galactosidase expression 10 days after gene transfer. Importantly, the E1/E4-AV vector produced substantially less endothelial cell activation, less inflammation, and reduced neointimal hyperplasia compared with the E1-AV vector
Senescent fibroblasts are known to promote tumor growth. However, the exact mechanism remains largely unknown. An important clue comes from recent studies linking autophagy with the onset of senescence. Thus, autophagy and senescence may be part of the same physiological process, known as the autophagy-senescence transition (AST). To test this hypothesis, human fibroblasts immortalized with telomerase (hTERT-BJ1) were stably transfected with autophagy genes (BNIP3, CTSB or ATG16L1). Their overexpression was sufficient to induce a constitutive autophagic phenotype, with features of mitophagy, mitochondrial dysfunction and a shift toward aerobic glycolysis, resulting in L-lactate and ketone body production. Autophagic fibroblasts also showed features of senescence, with increased p21(WAF1/CIP1), a CDK inhibitor, cellular hypertrophy and increased β-galactosidase activity. Thus, we genetically validated the existence of the autophagy-senescence transition. Importantly, autophagic-senescent ...
MITUSHI BIOPHARMA- Manufacturer & Supplier Of Alpha Galactosidase In Gujarat,India. Find More Detail For Alpha Galactosidase Manufacturers & Suppliers In Gujarat,India
Previously, we showed that C. glutamicum mycothiol peroxidase MPx, similar to the glutathione peroxidase (Gpx), was resistant to and induced by organic and inorganic peroxides [55]. Moreover, E. faecium gpx is regulated by MarR-type AsrR [44]. Thus, we suggested C. glutamicum MPx was regulated by CosR. The lacZ activity of Pmpx::lacZ chromosomal promoter fusion reporter in relevant C. glutamicum strains and quantitative real-time PCR (qRT-PCR) profiling of mpx expression were quantitatively measured in bacterial cells either untreated or treated with different toxic agents of various concentrations (Figure 5A,B). Concentrations of CHP applied were able to reduce the growth rate but under sub-lethal concentrations (Supplementary Figure S5). As expected, high levels of the promoter lacZ activity of mpx were detected in the ΔcosR strain, regardless of whether or not CHP was present. Under normal conditions (without CHP treatment), the promoter lacZ activity of mpx in ΔcosR strain was 6.5 times ...
Secreted frizzled related protein-1 (sFRP1), an antagonist of Wnt signaling, regulates cell proliferation, differentiation and apoptosis and negatively regulates bone formation. The spatial and temporal pattern of endogenous sFRP1 expression and loss-of-function were examined in the sFRP1-LacZ knock-in mouse (sFRP1-/-) during embryonic development and post-natal growth. beta-gal activity representing sFRP1 expression is robust in brain, skeleton, kidney, eye, spleen, abdomen, heart and somites in early embryos, but sFRP1 gene inactivation in these tissues did not compromise normal embryonic and post-natal development. Kidney histology revealed increased numbers of glomeruli in KO mice, observed after 5 years of breeding. In the skeleton, we show sFRP1 expression is found in relation to the mineralizing front of bone tissue during skeletal development from E15.5 to birth. Trabecular bone volume and bone mineral density in the sFRP1-/- mouse compared to WT was slightly increased during post-natal growth.
Isolation of cells differentially expressing lacZ activity: After development of the X-gal color for 2 wk, the membranes were photographed using Ektapan (Kodak, 4162) film and the resulting pairs of negatives were superimposed with a slight offset and examined by eye against a clear incandescent lightbulb. By applying strips of removable tape, the negatives were readily scanned for colonies putatively exhibiting differential lacZ expression. Colonies on the master plate were located by comparison with prints from the negatives. To purify the clones and document zinc-regulated lacZ expression, clones of interest were dispersed into 1 ml of low-zinc, Leuselective medium and 1 μl of this suspension spread as sectors on another low-zinc, Leu-selective plate. After colonies reached full size, the replica-plating procedure was repeated. About 70% of the clones exhibited perceptible differences in X-gal color in the two growth conditions and were kept for further study.. Identification of sequences ...
Full text for this publication is not currently held within this repository. Alternative links are provided below where available. ...
Prx1 and Prx2 are closely related paired-class homeobox genes that are expressed in very similar patterns predominantly in mesenchyme, Prx1 loss-of-function mutants show skeletal defects in skull, limbs and vertebral column (Martin, J, F,, Bradley, A, and Olson, E. N, (1995) Genes Dev. 9, 1237-1219). We report here that mice in which Prx2 is inactivated by a lacZ insertion had no skeletal defects, whereas Prx1/Prx2 double mutants showed many novel abnormalities in addition to an aggravation of the Prx1 single mutant phenotype. We found defects in external, middle and inner ear, reduction or loss of skull bones, a reduced and sometimes cleft mandible, and limb abnormalities including postaxial polydactyly and bent zeugopods, A single, or no incisor was present in the lower jaw, and ectopic expression of Fgf8 and Pax9 was found medially in the mandibular arch, A novel method to detect beta-galactosidase activity in hydroxyethylmethacrylate sections allowed detailed analysis of Prx2 expression in ...
Due to its role in aging and antitumor defense, cellular senescence has recently attracted increasing interest. However, [the] detection of senescent cells remains difficult due to the lack of specific biomarkers. ndeed, most determinants of cellular senescence, such as the upregulation of p53, p16Ink4a, p21WAF/CIP1 or SASP-associated cytokines, are not exclusively observed in senescence, but can also occur in other types of stress responses. In addition, alterations like SAHF or DNA-SCARS formation are frequently observed, but not necessarily a mandatory feature or exclusive to senescent cells. The current gold standard for the detection of senescence is the so-called senescence-associated β-galactosidase (SA-β-Gal) activity. Although SA-β-Gal has been first suggested as a distinct enzyme, its activity is derived from lysosomal β-Gal encoded by the GLB1 gene. β-Gal is an accepted marker of senescence, but its reliability and specificity have been questioned, as a positive β-Gal reaction ...
Homozygous Cdon|sup|lacZ-2|/sup| (or Cdo|sup|lacZ-2|/sup|) mice harbor a beta-galactosidase (|i|lacZ|/i|) knock-in mutation that also abolishes expression of the targeted |i|Cdon|/i| (cell adhesion molecule-related/down-regulated by oncogenes) gene. This strain may be useful in studying holoprosencephaly (HPE; a common defect of human forebrain development), craniofacial/brain development and the regulation of Sonic Hedgehog (Shh) signaling pathways.
Dazzling nude photos genetic yahoo transformation dating markers are related how to adult videos - Understand the uses of marker or reporter genes in molecular biology experiments and how Note: The pGREEN plasmid contains a mutant version of GFP linked to another gene called beta-galactosidase. . Make sure to also include your lab group name and the date. . Email Gmail AOL Mail boing12.info Yahoo Mail.
Cellular senescence is a normal consequence of aging, resulting from lifelong accumulation of DNA damage that triggers an end to cell replication. Although senescent cells no longer divide, they persist in their tissue of origin and develop characteristics that can hasten and exacerbate age-related disease. This series addresses the contribution of cellular senescence to cardiovascular, neurodegenerative, and arthritic disorders as well as the senescent phenotypes in various tissues and cell types. In addition to their cell-intrinsic effects, senescent cells develop the ability to negatively influence healthy neighboring cells and immune cells by secreting senescence-associated set of cytokines and mediators known as the SASP. These reviews also highlight ongoing efforts to accurately identify, target, and eliminate senescent cells or otherwise combat their deleterious effects in disease. One day, this work may provide the basis for therapies targeting aging cells in multiple organs.. ...
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putative UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase LOC402377-like; K03766 beta-1,3-N-acetylglucosaminyltransferase 5 [EC:2.4.1.206] ...
Cellular senescence, a term originally defining the characteristics of cultured cells that exceed their replicative limit, has been broadened to describe durable states of proliferative arrest induced by disparate stress factors. Proposed relationships between cellular senescence, tumour suppression, loss of tissue regenerative capacity and ageing suffer from lack of uniform definition and consistently applied criteria. Here, we highlight caveats in interpreting the importance of suboptimal senescence-associated biomarkers, expressed either alone or in combination. We advocate that more-specific descriptors be substituted for the now broadly applied umbrella term senescence in defining the suite of diverse physiological responses to cellular stress.
Although the induction of senescence in cancer cells is a potent mechanism of tumor suppression, senescent cells remain metabolically active and may secrete a broad spectrum of factors that promote tumorigenicity in neighboring malignant cells. Here we show that androgen deprivation therapy (ADT), a widely used treatment for advanced prostate cancer, induces a senescence-associated secretory phenotype in prostate cancer epithelial cells, indicated by increases in senescence-associated beta-galactosidase activity, heterochromatin protein 1 beta foci, and expression of cathepsin B and insulin-like growth factor binding protein 3. Interestingly, ADT also induced high levels of vimentin expression in prostate cancer cell lines in vitro and in human prostate tumors in vivo. The induction of the senescence-associated secretory phenotype by androgen depletion was mediated, at least in part, by down-regulation of S-phase kinase-associated protein 2, whereas the neuroendocrine differentiation of prostate ...
Purpose : ARPE-19 is currently used as an in vitro model for retinal diseases such as age-related degeneration (AMD). Up to now few studies show morphological and genetic expression differences between ARPE-19 and foetal or adult human retinal pigment epithelial (hRPE) cells. This study aims to compare ARPE-19 to hRPE cells derived from induced pluripotent stem cells (iPS) cells in both normal and oxidative stress condition (Fe-NTA treatment), the study hypothesis being to consider that hRPE-iPS cells are more able than ARPE-19 to model AMD in vitro. Methods : iPS cell obtained from peripheral venous blood samples of individuals aged more than 60 years were differenciated into hRPE cells (N=4). hRPE-iPS cells were characterized by morphology, immunofluorescence, FACS and RTqPCR. In basal condition, β-galactosidase activity was measured to quantify senescent cells. Cell count was performed manually with ZEN software. Fe-NTA added to the culture media was used to mimic oxidative environment. In ...
Most Pharmacological chaperones (PCs) described until now are substrate analogues which bind to the active site of the target protein. C ons e- quently, such PCs also inhibit the target protein at higher concentrations thus rendering a narrow therapeutic window and have poor drug-like properties. Through our proprietary technology platform SEE-Tx™, we identify a new generation of non-substrate competitive pharmacological chaperones which p o- tentially offer a much broader therapeutic window. Whats more, such co m- pounds are not substrate analogues, thus presenting much better drug-like pro p- erties, particularly for indications with CNS involvement. Here we present our methodology to identify non-competitive pharmacological chaperones applied to the enzyme beta-galactosidase, whose deficiency is related with GM1 Gangliosidosis and Morquio B ...
TY - JOUR. T1 - Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. AU - Wolff, Gerhard. AU - Worgall, Stefan. AU - Van Rooijen, Nico. AU - Song, Wen Ru. AU - Harvey, Ben Gary. AU - Crystal, Ronald G.. PY - 1997/1/1. Y1 - 1997/1/1. N2 - Based on the hypothesis that tissue macrophages present an obstacle for adenovirus (Ad) vector-mediated gene transfer to internal organs, this study evaluated the consequences of transient depletion of Kupffer cells on subsequent transfer of the Ad vector genome and Ad vector-directed gene expression in the livers of experimental animals. Depletion of Kupffer cells in mice by intravenous administration of multilamellar liposomes containing dichloromethylene-bisphosphonate permitted subsequent intravenous administration of an Ad vector to provide a higher input of recombinant adenoviral DNA to the liver, an absolute increase in transgene expression, and a delayed clearance of the ...

Systemic disease and the heart: Effects of enzyme-replacement therapy in patients with Anderson-Fabry disease: a prospective...Systemic disease and the heart: Effects of enzyme-replacement therapy in patients with Anderson-Fabry disease: a prospective...

Objective: This study aimed to assess the long-term effects of ERT with recombinant α-galactosidase A (agalsidase beta, ... Conclusions: Long-term therapy with agalsidase beta at 1 mg/kg every 2 weeks was effective in significantly reducing LV ... disease were examined by means of physical examination and magnetic resonance imaging before ERT with agalsidase beta at 1 mg/ ...
more infohttp://connection.ebscohost.com/c/articles/43671861/systemic-disease-heart-effects-enzyme-replacement-therapy-patients-anderson-fabry-disease-prospective-long-term-cardiac-magnetic-resonance-imaging-study

beta-galactosidase | Hackadaybeta-galactosidase | Hackaday

Posted in Medical HacksTagged adenovirus, beta-galactosidase, biohacking, enzyme, genetic engineering, lactase, lactose ... The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or ...
more infohttps://hackaday.com/tag/beta-galactosidase/

Beta-Galactosidase (Penicillium) - Drugs.comBeta-Galactosidase (Penicillium) - Drugs.com

A list of US medications equivalent to Beta-Galactosidase (Penicillium) is available on the Drugs.com website. ... Beta-Galactosidase (Penicillium) is a medicine available in a number of countries worldwide. ... Ingredient matches for Beta-Galactosidase (Penicillium). Tilactase. Beta-Galactosidase (Penicillium) (JAN) is also known as ... Beta-Galactosidase (Penicillium). Beta-Galactosidase (Penicillium) may be available in the countries listed below. ...
more infohttps://www.drugs.com/international/beta-galactosidase-penicillium.html

beta galactosidase in cultured cellsbeta galactosidase in cultured cells

... Martin Kennedy mkennedy at chmeds.ac.nz Tue Oct 5 21:23:18 EST 1993 *Previous message: ... with betagalactosidase constructs? ,, I am interested in staining the cells alive by adding Xgal to the culture ,, media ... Previous message: beta galactosidase in cultured cells *Next message: beta galactosidase in cultured cells ...
more infohttp://www.bio.net/bionet/mm/methods/1993-October/007940.html

is beta-galactosidase a glycoprotein?is beta-galactosidase a glycoprotein?

... Justin Kemp justin.news.invalid at web2news.net Wed Feb 26 23:31:21 EST 2003 *Previous ... I have been told that beta-galactosidase may be suitable. Can anyone tell me if it is indeed devoid of carbohydrate? Any help ...
more infohttp://www.bio.net/bionet/mm/proteins/2003-February/011018.html

Anti-beta Galactosidase antibody (ab106567) | AbcamAnti-beta Galactosidase antibody (ab106567) | Abcam

Chicken polyclonal beta Galactosidase antibody. Validated in WB and tested in Escherichia coli. Immunogen corresponding to ... Anti-beta Galactosidase antibody (ab106567) at 1 µg/ml. Lane 1 : beta Galactosidase at 0.005 µg. Lane 2 : beta Galactosidase at ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... 17 amino acid peptide from near the N terminus of beta Galactosidase from E. coli (NP_752394). ...
more infohttps://www.abcam.com/beta-galactosidase-antibody-ab106567.html

Beta-galactosidase jelly roll domain (IPR025300) | InterPro | EMBL-EBIBeta-galactosidase jelly roll domain (IPR025300) | InterPro | EMBL-EBI

Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.. J. Mol. Biol. 343 1281-92 2004 ... This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. It has a jelly roll fold [ ... Beta-galactosidase jelly roll domain (IPR025300). Short name: BetaGal_jelly_roll_dom ...
more infohttps://www.ebi.ac.uk/interpro/entry/IPR025300

Suicide gene therapy using E. coli beta-galactosidase.  - PubMed - NCBISuicide gene therapy using E. coli beta-galactosidase. - PubMed - NCBI

The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the ... Suicide gene therapy using E. coli beta-galactosidase.. Farquhar D1, Pan BF, Sakurai M, Ghosh A, Mullen CA, Nelson JA. ... Daun02 was a good substrate for beta-gal. By comparison, gal-DCN4 was a poor substrate. Except for PC3, the beta-gal-transduced ... O-beta- D-galactopyranosyl)butyl]daunorubicin (gal-DNC4) were investigated. The prodrugs were evaluated as substrates for beta- ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/12111114?dopt=Abstract

Recombinant E. coli  beta Galactosidase protein (ab79449)Recombinant E. coli beta Galactosidase protein (ab79449)

... beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols… ... Recombinant E. coli beta Galactosidase protein. See all beta Galactosidase proteins and peptides. ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be ...
more infohttp://www.abcam.com/recombinant-e-coli-beta-galactosidase-protein-ab79449.html

Beta-galactosidase - OpenWetWareBeta-galactosidase - OpenWetWare

High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques. 2004 Mar;36(3):410-5. PubMed ... Uses of lacZ to study gene function: evaluation of beta-galactosidase assays employed in the yeast two-hybrid system. Anal ... Ullmann A. Complementation in beta-galactosidase: from protein structure to genetic engineering. Bioessays. 1992 Mar;14(3):201- ... Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the ...
more infohttps://openwetware.org/wiki/?title=Beta-galactosidase&oldid=194455

bgaC - Beta-galactosidase - Bacillus circulans - bgaC gene & proteinbgaC - Beta-galactosidase - Bacillus circulans - bgaC gene & protein

Beta-galactosidaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,O31341,O31341_BACCI Beta-galactosidase OS=Bacillus circulans GN=bgaC PE=1 SV=1 ...
more infohttp://www.uniprot.org/uniprot/O31341

RCSB PDB - Protein Feature View 









 - Beta-galactosidase - A0A0B4U8I5 (A0A0B4U8I5 9GAMM)RCSB PDB - Protein Feature View - Beta-galactosidase - A0A0B4U8I5 (A0A0B4U8I5 9GAMM)

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
more infohttp://www.rcsb.org/pdb/protein/A0A0B4U8I5

RCSB PDB - Protein Feature View 









 - Beta-galactosidase - P00722 (BGAL ECOLI)RCSB PDB - Protein Feature View - Beta-galactosidase - P00722 (BGAL ECOLI)

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
more infohttps://www.rcsb.org/pdb/protein/P00722

Beta-Galactosidase Assay, β-Galactosidase StainingBeta-Galactosidase Assay, β-Galactosidase Staining

... and a complete beta-galactosidase reporter system. Fast, simple assay for beta-galactosidase activity. ... Colorimetric and chemiluminescent assays for beta galactosidase, LacZ vectors, ... Beta-Galactosidase Assay & LacZ Vectors. The beta-galactosidase assay is commonly used as a reporter, to monitor transfection ... Complete Beta-Gal Reporter System. The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta- ...
more infohttp://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Chemiluminescent_Reporters/Beta_Galactosidase?sitex=10020:22372:US&PROD=9yPYDt14bJ5SW72xBRbaSJSZ:S&PROD_pses=ZG447B1476FE55AE458E79C4F30DE21259B7B1F0D9AE57FDD7EE06DDAC11BF167F43772CFCA83AB04373E258B5D4EFCC6E618DBB9B33E45A83

Beta-Galactosidase Assay, β-Galactosidase StainingBeta-Galactosidase Assay, β-Galactosidase Staining

... and a complete beta-galactosidase reporter system. Fast, simple assay for beta-galactosidase activity. ... Colorimetric and chemiluminescent assays for beta galactosidase, LacZ vectors, ... Beta-Galactosidase Assay & LacZ Vectors. The beta-galactosidase assay is commonly used as a reporter, to monitor transfection ... Complete Beta-Gal Reporter System. The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta- ...
more infohttp://www.clontech.com/FR/Products/Fluorescent_Proteins_and_Reporters/Chemiluminescent_Reporters/Beta_Galactosidase

Beta-galactosidase - WikipediaBeta-galactosidase - Wikipedia

A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... proposed a new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) ... "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111/j.1474- ... β-galactosidase synthesis stops when glucose levels are sufficient. Beta-galactosidase has many homologues based on similar ...
more infohttps://en.wikipedia.org/wiki/Beta-galactosidase

Beta-Galactosidase: Properties, Structure and Functions - Nova Science PublishersBeta-Galactosidase: Properties, Structure and Functions - Nova Science Publishers

β-galactosidase is found in plants, animals and microorganisms. In Beta-Galactosidase: Properties, Structure and Functions, the ... Beta-Galactosidase: Properties, Structure and Functions. Eloy Kras (Editor). Series: Cell Biology Research Progress. BISAC: ... Home / Shop / Books / Science and Technology / Life Sciences / Beta-Galactosidase: Properties, Structure and Functions. ... β-galactosidase is an enzyme responsible for catalyzing the hydrolysis of the lactose β-1,4 linkage into α-D-glucose and β-D- ...
more infohttps://novapublishers.com/shop/beta-galactosidase-properties-structure-and-functions/

Beta-galactosidase Screen - OpenWetWareBeta-galactosidase Screen - OpenWetWare

These protocols are used to screen bacterial plates for beta-galactosidase activity using X-Gal (aka blue white screening). ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Beta-galactosidase_Screen&oldid=537279" ...
more infohttps://openwetware.org/wiki/Beta-galactosidase_Screen

beta Galactosidase Antibody (MA1-152)
                
                
		        
	beta Galactosidase Antibody (MA1-152)

Invitrogen Anti-beta Galactosidase Monoclonal (DC1 4C7), Catalog # MA1-152. Tested in Western Blot (WB), Immunofluorescence (IF ... Cite beta Galactosidase Monoclonal Antibody (DC1 4C7). The following antibody was used in this experiment: beta Galactosidase ... Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be ... coli beta Galactosidase at ~110 kDa. This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells. ...
more infohttps://www.thermofisher.com/antibody/product/beta-Galactosidase-Antibody-clone-DC1-4C7-Monoclonal/MA1-152

beta-Galactosidase | Harvard Catalyst Profiles | Harvard Catalystbeta-Galactosidase | Harvard Catalyst Profiles | Harvard Catalyst

... "beta-Galactosidase" by people in Harvard Catalyst Profiles by year, and whether "beta-Galactosidase" was a major or minor topic ... non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1 ... "beta-Galactosidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "beta-Galactosidase" by people in Profiles. ...
more infohttps://connects.catalyst.harvard.edu/Profiles/display/Concept/beta-Galactosidase

Senescence-associated beta-galactosidase is lysosomal beta-galactosidase.  - PubMed - NCBISenescence-associated beta-galactosidase is lysosomal beta-galactosidase. - PubMed - NCBI

Senescence-associated beta-galactosidase is lysosomal beta-galactosidase.. Lee BY1, Han JA, Im JS, Morrone A, Johung K, Goodwin ... SA-beta-gal induction during senescence was due at least in part to increased expression of the lysosomal beta-galactosidase ... We demonstrate here that SA-beta-gal activity is expressed from GLB1, the gene encoding lysosomal beta-D-galactosidase, the ... The most widely used biomarker for senescent and aging cells is senescence-associated beta-galactosidase (SA-beta-gal), which ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/16626397?dopt=Abstract

Beta-galactosidase (Sinorhizobium meliloti) | Protein Target - PubChemBeta-galactosidase (Sinorhizobium meliloti) | Protein Target - PubChem

Protein target information for Beta-galactosidase (Sinorhizobium meliloti). Find diseases associated with this biological ...
more infohttps://pubchem.ncbi.nlm.nih.gov/protein/Q59750

Endo-beta-galactosidase - WikipediaEndo-beta-galactosidase - Wikipedia

Endo-beta-galactosidase may refer to: Blood-group-substance endo-1,4-beta-galactosidase Keratan-sulfate endo-1,4-beta- ...
more infohttps://en.wikipedia.org/wiki/Endo-beta-galactosidase

Beta-Galactosidase Staining Kit (transfection efficiency)Beta-Galactosidase Staining Kit (transfection efficiency)

The Beta-Galactosidase Staining Kit makes it easy to determine the transfection efficiency of mammalian cells using co- ... Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit.. One µg of a 119 kDa subunit of β-galactosidase was ... The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lacZ ... The gene product of lacZ, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily ...
more infohttps://www.activemotif.com/catalog/40/%CE%B2-galactosidase-staining-kit

lacZ - Beta-galactosidase - Escherichia coli O157:H7 - lacZ gene & proteinlacZ - Beta-galactosidase - Escherichia coli O157:H7 - lacZ gene & protein

Beta-galactosidaseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... sp,Q8X685,BGAL_ECO57 Beta-galactosidase OS=Escherichia coli O157:H7 OX=83334 GN=lacZ PE=3 SV=1 ... Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation. ,p>Manual validated ... IPR036156. Beta-gal/glucu_dom_sf. IPR011013. Gal_mutarotase_sf_dom. IPR008979. Galactose-bd-like_sf. IPR014718. GH-type_carb-bd ...
more infohttp://www.uniprot.org/uniprot/Q8X685
  • We demonstrate here that SA-beta-gal activity is expressed from GLB1, the gene encoding lysosomal beta-D-galactosidase, the activity of which is typically measured at acidic pH 4.5. (nih.gov)
  • In addition, late passage normal fibroblasts expressing small-hairpin interfering RNA that depleted GLB1 mRNA underwent senescence but failed to express SA-beta-gal. (nih.gov)
  • GLB1 mRNA depletion also prevented expression of SA-beta-gal activity in HeLa cervical carcinoma cells induced to enter a senescent state by repression of their endogenous human papillomavirus E7 oncogene. (nih.gov)
  • Fibroblasts from patients with autosomal recessive G(M1)-gangliosidosis, which have defective lysosomal beta-galactosidase, did not express SA-beta-gal at late passages even though they underwent replicative senescence. (nih.gov)
  • The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikipedia.org)
  • Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells. (thermofisher.com)
  • In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikipedia.org)
  • Mutations in some of the codons of the N-terminal 60aa or C-terminal 100aa results in an inactive, dimeric β-galactosidase. (openwetware.org)
  • pCMV-LacZ is a constitutive mammalian reporter vector which expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter ( P CMV IE ) and can be used as a reference or control plasmid. (clontech.com)
  • A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase. (openwetware.org)