A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
An enzyme that catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-galactosides including galactose oligosaccharides, galactomannans, and galactolipids.
A 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by COMPLEMENT FACTOR I into C3c (749-954), and C3dg (955-1303) in the presence COMPLEMENT FACTOR H.
Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.
A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)
Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
A surgical specialty concerned with the study and treatment of disorders of the ear, nose, and throat.
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)
Vaccines used in conjunction with diagnostic tests to differentiate vaccinated animals from carrier animals. Marker vaccines can be either a subunit or a gene-deleted vaccine.
The inactive form of GLYCOGEN PHOSPHORYLASE that is converted to the active form PHOSPHORYLASE A via phosphorylation by PHOSPHORYLASE KINASE and ATP.
The sum of the weight of all the atoms in a molecule.
A class of glucosyltransferases that catalyzes the degradation of storage polysaccharides, such as glucose polymers, by phosphorolysis in animals (GLYCOGEN PHOSPHORYLASE) and in plants (STARCH PHOSPHORYLASE).
The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Includes ortho-, meta-, and para-nitrophenylgalactosides.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Method of making images on a sensitized surface by exposure to light or other radiant energy.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.

Fecal coliform elevated-temperature test: a physiological basis. (1/7076)

The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.5 degrees C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5 degrees C. A temperature increase from 35 to 44.5 degrees C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform beta-galactosidase activity coupled with thermal inactivation of this enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.  (+info)

The effects of digestive enzymes on characteristics of placental insulin receptor. Comparison of particulate and soluble receptor preparations. (2/7076)

The role of the surrounding membrane structure on the binding characteristics of the insulin receptor was studied by using several digestive enzymes. The effects observed with particulate membrane preparations are compared with those from soluble receptor preparations. beta-Galactosidase and neuraminidase had no effect on insulin binding to either particulate or soluble receptors from human placentae. Exposure to 2 units of phospholipase C/ml increased insulin binding to particulate membranes, but was without effect on the soluble receptor preparation. The increase in binding to particulate membranes was shown to be due to an increase in apparent receptor number. After 5 min exposure to 500 microgram of trypsin/ml there was an increase in insulin binding to the particulate membrane fraction, owing to an increase in receptor affinity. After 15 min exposure to this amount of trypsin, binding decreased, owing to a progressive decrease in receptor availability. In contrast, this concentration of trypsin had no effect on the solubilized receptor preparation. Because of the differential effects of phospholipase C and trypsin on the particulate compared with the solubilized receptor preparations, it is concluded that the effects of these enzymes were due to an effect on the surrounding membrane structure. Changes in receptor configuration due to alterations within the adjoining membrane provide a potential mechanism for mediating short-term alterations in receptor function.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (3/7076)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (4/7076)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Pathogenicity island 2 mutants of Salmonella typhimurium are efficient carriers for heterologous antigens and enable modulation of immune responses. (5/7076)

The potential use as vaccine delivery system of Salmonella typhimurium strains harboring defined mutations in the sseC (HH104) and sseD (MvP101) genes, which encode putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2, was evaluated and compared with that of the well-characterized aroA mutant strain SL7207 by using beta-galactosidase (beta-Gal) as a model antigen. When orally administered to immune-competent or gamma interferon-deficient (IFN-gamma-/-) BALB/c mice, both mutants were found to be highly attenuated (50% lethal dose, >10(9) bacteria). Both strains were also able to efficiently colonize and persist in Peyer's patches. Immunization with HH104 and MvP101 triggered beta-Gal-specific serum and mucosal antibody responses equivalent to or stronger than those observed in SL7207-immunized mice. Although immunoglobulin G2 (IgG2) serum antibodies were dominant in all groups, IgG1 was also significantly increased in mice vaccinated with MvP101 and SL7207. Comparable beta-Gal-specific IgA and IgG antibodies were detected in intestinal lavages from mice immunized with the different strains. Antigen-specific CD4(+) T-helper cells were generated after vaccination with all vaccine prototypes; however, responses were significantly more efficient when HH104 and MvP101 were used (P < 0.05). Significantly higher levels of IFN-gamma were produced by restimulated spleen cells from mice immunized with HH104 than from those vaccinated with the MvP101 or SL7207 derivatives (P +info)

Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes. (6/7076)

BACKGROUND: In congestive heart failure, high systemic levels of the hormone arginine vasopressin (AVP) result in vasoconstriction and reduced cardiac contractility. These effects are mediated by the V1 vasopressin receptor (V1R) coupled to phospholipase C beta-isoforms. The V2 vasopressin receptor (V2R), which promotes activation of the Gs/adenylyl cyclase system, is physiologically expressed in the kidney but not in the myocardium. Expression of a recombinant V2R (rV2R) in the myocardium could result in a positive inotropic effect via the endogenous high concentrations of AVP in heart failure. METHODS AND RESULTS: A recombinant adenovirus encoding the human V2R (Ad-V2R) was tested for its ability to modulate the cardiac Gs/adenylyl cyclase system and to potentiate contractile force in rat ventricular cardiomyocytes and in H9c2 cardiomyoblasts. Ad-V2R infection resulted in a virus concentration-dependent expression of the transgene and led to a marked increase in cAMP formation in rV2R-expressing cardiomyocytes after exposure to AVP. Single-cell shortening measurements showed a significant agonist-induced contraction amplitude enhancement, which was blocked by the V2R antagonist, SR 121463A. Pretreatment of Ad-V2R-infected cardiomyocytes with AVP led to desensitization of the rV2R after short-term agonist exposure but did not lead to further loss of receptor function or density after long-term agonist incubation, thus demonstrating resistance of the rV2R to downregulation. CONCLUSIONS: Adenoviral gene transfer of the V2R in cardiomyocytes can modulate the endogenous adenylyl cyclase-signal transduction cascade and can potentiate contraction amplitude in cardiomyocytes. Heterologous expression of cAMP-forming receptors in the myocardium could lead to novel strategies in congestive heart failure by bypassing the desensitized beta-adrenergic receptor signaling.  (+info)

Multiple cis-acting regulatory regions are required for restricted spatio-temporal Hoxa5 gene expression. (7/7076)

Genetic analyses have revealed the essential role of the murine Hoxa5 gene for the correct specification of the cervical and upper thoracic region of the skeleton, and for the normal organogenesis and function of the respiratory tract, both structures expressing Hoxa5 during embryogenesis. To understand how the expression domains of the Hoxa5 gene are established during development, we have analyzed the cis-acting control regions mediating Hoxa5 gene expression using a transgenic approach. Four transcripts are derived from the Hoxa5 locus. The shortest and most abundant one displays a specific spatio-temporal profile of expression at earlier stages and in more anterior structures along the embryonic axis than the larger forms. We established that an 11.1 kilobase pair (kb) genomic fragment, extending from position -3.8 kb to +7.3 kb relative to Hoxa5 transcription initiation site, was sufficient to reproduce the temporal expression and substantially reconstitute the spatial pattern of the major Hoxa5 transcript. By deletion analyses, we identified a 2.1 kb fragment located downstream of the Hoxa5 gene that possesses mesodermal enhancer activity. Overall, the findings demonstrate that cis-acting regulatory elements essential for the correct expression of the major Hoxa5 transcript are located both upstream and downstream of the Hoxa5 coding sequences.  (+info)

Ectopic expression of the transforming growth factor beta type II receptor disrupts mesoderm organisation during mouse gastrulation. (8/7076)

Transforming growth factor beta (TGFbeta) regulates the cell cycle and extracellular matrix (ECM) deposition of many cells in vitro. We have analysed chimaeric mouse embryos generated from embryonic stem cells with abnormal receptor expression to study the effect of TGFbeta on these processes in vivo and the consequences for normal development. The binding receptor for TGFbeta, TbetaRII, is first detected in the embryo proper around day 8.5 in the heart. Ectopic expression of TbetaRII from the blastocyst stage onward resulted in an embryonic lethal around 9.5 dpc. Analysis of earlier stages revealed that the primitive streak of TbetaRII chimaeras failed to elongate. Furthermore, although cells passed through the streak and initially formed mesoderm, they tended to accumulate within the streak. These defects temporally and spatially paralleled the expression of the TGFbeta type I receptor, which is first expressed in the node and primitive streak. We present evidence that classical TGFbeta-induced growth inhibition was probably the cause of insufficient mesoderm being available for paraxial and axial structures. The results demonstrate that (1) TGFbeta mRNA and protein detected previously in early postimplantation embryos is present as a biologically active ligand; and (2) assuming that ectopic expression of TbetaRII results in no other changes in ES cells, the absence of TbetaRII is the principle reason why the embryo proper is unresponsive to TGFbeta ligand until after gastrulation.  (+info)

Beta Galactosidase | Learn more about Beta Galactosidase | Meaning of Beta Galactosidase | Description of Beta Galactosidase | Details of Beta Galactosidase | Article on Beta Galactosidase | Essay on Beta Galactosidase | Definition of Beta Galactosidase | Infospaze
microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
Quantitation of β-galactosidase activity. In yeast cells, co-transformed with pGADT7 (AD) and pGBKT7 (BD) constructs as indicated, β-galactosidase activity wa
Buy our Recombinant |em|E. coli |/em| beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols…
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
BioTek Notes dapplication, 26-Feb-07, Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine Leukemia- ...
|strong|Rabbit anti beta-galactosidase polyclonal antibody|/strong| detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccha…
BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.) ...
METHODS AND RESULTS The method involves the harvest of autologous venous-derived endothelial cells, the efficient genetic modification of the cells through the use of recombinant retroviruses, and the subsequent implantation of the genetically modified cells on the surface of balloon-denuded arterial segments. With a rabbit model, freshly isolated endothelial cells were transduced with a recombinant retrovirus encoding the bacterial enzyme beta-galactosidase. The autologous transduced cells were then implanted on the surface of balloon-denuded ileofemoral arterial segments at different cell densities; after 1 to 14 days, the animals were killed, and the vessel segments were examined. Cells expressing the bacterial gene product, as determined by in situ staining for beta-galactosidase, were found to be present on the surface of 28 of the 32 arteries seeded with genetically modified cells. Vessels examined at 4 to 7 days after seeding displayed 40% to 90% coverage with transduced cells, even when ...
Beta-galactosidase, 0.1 ml. This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates.
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic units (monosaccharides). - Stock Image C015/1970
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade Quantity:
1DP0: High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.
The cae8 promoter is sensitive to the amino acid L-asparagine.The figure shows measured β-galactosidase activities from wild-type BW25113 cells, carrying the c
Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C
C-terminal BetaGal reporter tag yeast expression plasmid. This vector allows the creation of reporter fusion proteins with no protease cleavage tag.
lacZ. No transcription. Structural genes lacZ. lacY. lac A. No transcription. Presence of lactose. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor). Allolactose. 12 When lactose is present, some of it is converted into allolactose,.... RNA polymerase lacO operator. Transcription and translation. Active regulator protein. Inactive regulator protein (repressor) ...
Misc.Comments : The SK designation indicates the polylinker is oriented such that beta-galactosidase (lacZ) transcription proceeds through the SacI site first and the KpnI site last. pBluescript SK(+) carries an F1 origin of replication, oriented such that transcription proceeds in the same direction as beta-galactosidase (lacZ) transcription ...
BioAssay record AID 404864 submitted by ChEMBL: Activation of human estrogen receptor beta expressed in HEK293 cells at 1 nM beta-galactosidase reporter gene assay relative to control.
Old cells, new tricks: chromatin structure in senescence | SpringerLink. Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) | Abcam. Overview of Cellular Senescence and Aging | Cell Signaling Technology. The chemistry of senescence | Nature Reviews Chemistry. Infographic: How Does Cell Senescence Drive Aging and Disease? | The Scientist Magazine®. Cellular senescence is associated with age-related blood clots. Frontiers | Cellular Senescence as the Causal Nexus of Aging | Genetics. Diabetic Retinopathy: premature cellular senescence and microvascular dysfunction - ELISA Genie. Stem Cells Versus Senescence | JACC: Journal of the American College of Cardiology. MHY2233 Attenuates Replicative Cellular Senescence in Human Endothelial Progenitor Cells via SIRT1
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase ...
Substâncias complexas são convertidas, pela ação de enzimas, em moléculas solúveis, durante o processo de germinação, as quais são translocadas para a plântula em crescimento, servindo como fonte de energia ou estrutura física. Com o objetivo de quantificar a atividade enzimática da -galactosidase e a mobilização de mono e oligossacarídeos durante o período de germinação, foi conduzido este estudo utilizando-se sementes de jacarandá-da-bahia. As sementes foram mantidas em germinador a 25oC sob luz contínua, sendo avaliada a protrusão da radícula, pelo período de 10 dias. Foram quantificados os teores de mono e de oligossacarídeos, assim como a atividade específica da enzima -galactosidase nos tempos zero, um, três e cinco dias. Houve mobilização das reservas de glicose e manose nos cotilédones e xilose neste e no embrião nos três primeiros dias de germinação. A ramnose teve os teores aumentados nos cotilédones e no eixo embrionário. A rafinose foi o ...
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
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You might also use the Kyoto Encyclopedia of Genes and Genomes, which has a lot of information conveniently summarized for many proteins; for example, the entry for the E. coli beta-galactosidase gene is here. Note that the entries for orthologies, pathways, motifs, cross-references to other databases, structures, etc. are all clickable.. ...
It was found that Concanavalin A (Con A) accelerates the rates of hydrolysis of E. coli beta-galactosidase and yeast invertase by binding to the product (glucose) formed in the reaction. The effect of Con A can be made ...
The adsorption of beta-galactosidase (BG) from solution by anti-beta-galactosidase monoclonal antibody (ABG) immobilized on nonporous, glass coated beads has been studied in a stirred batch adsorber at 4, 8, 15, and 20°C and for different initial BG concentrations. The elution of BG from the BG-ABG complex was also examined. The experiments performed in this work provided data from which information may be obtained about the influence of the concentration of BG and of the temperature on the dynamics of the adsorption of BG onto immobilized ABG, as well as on the total amount of BG adsorbed at equilibrium. Such experimental data may be used in studies involving the modelling of the kinetics of adsorption of BG onto immobilized ABG--Abstract, page ii.
BioAssay record AID 142597 submitted by ChEMBL: Agonistic efficacy in human m1 muscarinic receptor which was expressed with the marker gene beta-galactosidase in NIH 3T3 cells. Maximal effect normalized to the effect of carbachol (%CCH)..
Interestingly, elimination of PA5471.1 translation via an M1T mutation also enhanced PA5471 expression, reminiscent of the effects of eliminating the pBC16 tet LP ribosome binding site (i.e., no translation of the tet gene possible) where Tet protein expression was enhanced 10-fold and was tetracycline unresponsive (29). It is unclear, however, how lack of PA5471.1 translation would provide the same signal as ribosome stalling, although in the case of the M1T mutation the ribosomes may be pausing at the ribosome binding site in the absence of a start codon.. The observation that fusion strains carrying 3′-truncated fragments of the PA5471 upstream region lacking a putative attenuator (Fig. 1, K2809, K2792, and K2794) yielded less β-galactosidase activity than full-length upstream fragments containing the attenuator sequences but carrying a nonsense mutation (Q3Am) in PA5471.1 (Fig. 1, K2800) was puzzling given the expectation that an attenuator-minus sequence would, in theory, provide for ...
The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert presence of insert results into insertional inactivation of Beta galactosidase and recombinant colonies do not produce any colour these are identified as Recombinant Colony
Cellular senescence is a fundamental cell fate playing significant and complex roles during tumorigenesis and natural aging process. However, the molecular determinants distinguishing senescence from other temporary and permanent cell-cycle arrest states such as quiescence and post-mitotic state and the specified mechanisms underlying cell-fate decisions towards senescence versus cell death in response to cellular stress stimuli remain less understood. In our studies, we aimed to employ multi-omics approaches to deepen our understanding of cellular senescence, in particular, regarding the specific molecular determinants distinguishing cellular senescence from other non-dividing cell fates. Notably, one of the most prominent features of cellular senescence differing from other non-dividing cell fates is the increased expression of senescence-associated beta-galactosidase. Because 5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside (C12FDG) is known as the substrate catalyzed by ...
A Q: for transfection gurus. We are measuring promoter activity using luciferase gene as a main reporter, fused to the putative promoter fragments, and CMV/beta-galactosidase gene as a second, normalizer activity. Both are introduced by lipofection into an aortic cell line. Luciferase activity is measured using a Promega kit, and the b-gal is measured via a chemiluminescence based kit from Tropix (GalactoLight). Before measuring the latter activity we heat-inactivate the endogenous activity. What we get is that for a given construct the luciferase activity in a 3X transfection series is relatively constant ( within a factor of 1.5), but the respective set of b-gal activities is variable within a factor of 3, which ruins the experiment. Any ideas? Has anyone used GalactoLight with success? -- Alexander Kraev, PhD Biochemie III, ETHZ Zurich Phone 41-1-632-31-47 Fax 41-1-632-12-13 e-mail kraev at bc.biol.ethz.ch ...
Using a fungus two-hybrid system, we isolated a book human centrosomal protein, CPAP (centrosomal P4. utilized to display screen for protein that connect to 4.1R-135. The top domains (HD; residues 1 to 209) of 4.1R-135 (4.1R-HD) was fused towards the GAL4 DNA-binding domains (GAL4-DB) in vector pAS2-1 (Clontech). This create was used as bait to display a Z-VAD-FMK inhibition human being lymphocyte cDNA library fused to a GAL4 activation website (GAL4-AD) in the pACT2 vector (Clontech). The two types of plasmids were then cotransformed into Y190, and the transformants were selected on SD minimal medium as previously explained (40). Positive colonies were further tested for -galactosidase activity using a colony-lift assay and liquid assay as explained by the manufacturer (Clontech). To thin down the head website region of 4.1R (4.1R-HD) that binds to CPAP, constructs containing numerous portions of 4.1R-HD were fused to GAL4-DB of the pAS2-1 vector (Fig. ?(Fig.1A).1A). The C terminus of CPAP ...
Dimerization data (column 1) represent relative binding to GST-TBP(181C) as determined in Figure 1E and are an average of six sets of data. See Figure 5 for standard errors. β-galactosidase activity (columns 3-6) is relative to wild-type test TBP in column 3 (1.0 = 3 Miller units using the high-sensitivity substrate CPRG) and represents an average of at least three independent determinations. All data were obtained in the linear range of the assay. The null allele expresses only the first 81 amino acids of TBP. Test HA-tagged TBP levels were determined by quantitative Western blotting with TBP antibodies as described in Figure 5B. TBP levels are relative to the endogenous (untagged) TBP (1 = 17,000 ± 2,000 molecules per cell). The standard error for all data is presented graphically in Figure 5E. Expression of the TBPEBmutants did not cause a decrease in the expression of TAFII145 (data not shown). Low in columns 3 and 7 indicates that the test TBP was driven by the TBP promoter; high in ...
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The results of this study are consistent with the following conclusions. First, unconjugated β-galactosidase is rapidly cleared from blood in vivo (Table 2), owing to rapid uptake of the unconjugated enzyme by liver and spleen (Figs. 2 and 3). Second, once inside cells, β-galactosidase is rapidly degraded in vivo such that 99% of the organ enzyme activity is lost at 4 h after an intravenous injection (Table 1). Third, the 116-kDa β-galactosidase (Fig. 1B) can be conjugated to the 8D3 TfRmAb without loss of enzyme activity (Fig. 1C). Fourth, there is minimal brain uptake of the unconjugated β-galactosidase, but there is a 10-fold increase in brain uptake of enzyme following conjugation to the 8D3 TfRmAb (Table 1; Figs. 2 and 3).. The β-galactosidase is rapidly removed from the blood due to the avid uptake of the enzyme by liver and spleen (Figs. 2 and 3), which confirms the earlier observation of Onodera et al. (1983). The blood concentration of the β-galactosidase-TfRmAb is 5- to 10-fold ...
ATCC ® 77267™ Designation: pUN65 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
ATCC ® 77269™ Designation: pUN90 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Edvotek 300 kit shows how to clone a DNA fragment by using ligation, transformation and an assay of ß-galactosidase. For advanced students.
Page contains details about β-galactosidase/ZIF-8 MOF coating . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Expresses the head domain of Nod fused to the coiled-coil domain of KHC and beta-galactosidase under the control of UAS; the fusion protein accumulates at the minus ends of microtubules, N.G ...
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols. ...
reacts with native and denatured-reduced E. coli β-galactosidase(116 kD); may be usedfor detection of β-galactosidase expressed by E. colilacZ gene encoded in many cloned gene sequences,and serves as an indicator for fusion proteins encodedby an inserted DNA ...
Langley, K. E.; Villarejo, M. R.; Fowler, A. V.; Zamenhof, P. J.; Zabin, I. (1975). "Molecular basis of beta-galactosidase ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ...
"Molecular basis of beta-galactosidase alpha-complementation". Proceedings of the National Academy of Sciences of the United ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ...
1984). "Morquio B syndrome: a primary defect in beta-galactosidase". Am. J. Med. Genet. 16 (2): 261-75. doi:10.1002/ajmg. ... 1994). "Normal serum beta-galactosidase in juvenile GM1 gangliosidosis". Pediatr. Neurol. 10 (4): 317-9. doi:10.1016/0887-8994( ... Activation, stabilization and association with beta-galactosidase and its protective protein". Eur. J. Biochem. 149 (2): 315-21 ... This gene encodes a glycoprotein that associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex ...
LacZ (beta-galactosidase)[10]. *Luciferase,[11][12] including ReBiL (recombinase enhanced bimolecular luciferase)[13] and ... "Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation". Proceedings of the ... Park JH, Back JH, Hahm SH, Shim HY, Park MJ, Ko SI, Han YS (October 2007). "Bacterial beta-lactamase fragmentation ... Remy I, Ghaddar G, Michnick SW (2007). "Using the beta-lactamase protein-fragment complementation assay to probe dynamic ...
... such as the gene coding for beta-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into ...
MCS located within the lac Z gene causes insertional inactivation of this gene at the N-terminal fragment of beta-galactosidase ... Notably, it has an N-terminal fragment of β-galactosidase (lacZ) gene of E. coli. The multiple cloning site (MCS) region is ... The lacZ gene codes for β-galactosidase. The recognition sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and ... In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (ampR), via a β-lactamase enzyme that ...
Earliest discoveries of transferase activity occurred in other classifications of enzymes, including Beta-galactosidase, ...
On the other hand, if it is a beta-galactoside, it is called beta-galactosidase, and is responsible for breaking down the ... and agalsidase beta (INN). Lack of alpha-galactosidase activity in leukocytes has been linked to Fabry Disease. Galactosidases ... B-galactosidase forms the basis of lac z operon in bacteria which can be used to control gene expression. B-galactosidase can ... B-galactosidase is made up of 4 identical polypeptide chains, i.e. it has 4 identical subunits. When B-galactosidase is ...
Hengstenberg, W., Penberthy, W.K. and Morse, M.L. (1970). "Purification of the staphylococcal 6-phospho-β-D-galactosidase". Eur ... 6-Fosfo-beta-galaktozidaza (EC 3.2.1.85, fosfo-beta-galaktozidaza, beta-D-fosfogalaktozid galaktohidrolaza, fosfo-beta-D- ... galaktozidaza, 6-fosfo-beta-D-galaktozidaza) je enzim sa sistematskim imenom 6-fosfo-beta-D-galaktozid 6-fosfogalaktohidrolaza. ... 6-fosfo-beta-D-galaktozid + H2O ⇌. {\displaystyle \rightleftharpoons }. 6-fosfo-D-galaktoza + alkohol. Reference[uredi - уреди ...
... modeling beta-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta. 1770 (5): 733-46. doi:10.1016/j.bbagen. ...
Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase- ...
... beta-D-glucosidase, beta-glucoside glucohydrolase, arbutinase, amygdalinase, p-nitrophenyl beta-glucosidase, primeverosidase, ... Beta-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and ... Vicianin beta-glucosidase. References[edit]. *^ PDB: 3AHX​; Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang ... amygdalase, linamarase, salicilinase, and beta-1,6-glucosidase. Cellulose is a polymer composed of beta-1,4-linked glucosyl ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
In S-glycosylation, a beta-GlcNAc is attached to the sulfur atom of a cysteine residue.[4] ... Beta-galactosidase. *Hexosaminidase. *mannosidase *alpha-Mannosidase. *beta-mannosidase. *Aspartylglucosaminidase. *Fucosidase ...
Beta-galactosidase. *Beta-glucosidase. *Beta-ketoacyl-ACP synthase. *Beta-secretase. *Betaine-homocysteine S-methyltransferase ...
Beta ketothiolase deficiency. *Beta-galactosidase-1 deficiency. *Beta-mannosidosis. *Beta-sarcoglycanopathy. *Beta-thalassemia ...
Beta-galactosidase (EC 3.2.1.23). *Hyaluronidase (EC 3.2.1.35). Category:EC 3.3 (act on ether bonds)Edit. *Category:EC 3.3 ... EC 6.2.1.28: 3-alpha,7-alpha-dihydroxy-5-beta-cholestanate--CoA ligase ...
One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved ...
The enzyme alpha-galactosidase A (α-GalA) Globotriaosylceramide (Gb3), a substrate of α-GalA, has a terminal D-galactose ... When combined with intravenous agalsidase alfa or beta, which are recombinant versions of the enzyme α-GalA, migalastat ... 2015). "Oral Migalastat HCl Leads to Greater Systemic Exposure and Tissue Levels of Active α-Galactosidase A in Fabry Patients ... ISBN 978-0-470-03391-3. Miyake Y, Ebata M (1988). "The structures of a β-galactosidase inhibitor, Galactostatin, and its ...
alpha-galactosidase (Agalsidase alfa. *Agalsidase beta). *Glycosaminoglycan: iduronidase (Laronidase). *arylsulfatase B ( ... A16AB04 Agalsidase beta. A16AB05 Laronidase. A16AB06 Sacrosidase. A16AB07 Alglucosidase alfa. A16AB08 Galsulfase. A16AB09 ... A16AB16 Idursulfase beta. A16AB17 Cerliponase alfa. A16AB18 Vestronidase alfa. A16AX Various alimentary tract and metabolism ...
Galactosidases *Alpha. *Beta. *alpha-Mannosidase. *Glucuronidase *Klotho. *Hyaluronidase. *Pullulanase. *Glucosylceramidase * ...
... beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase), ... Separation and characterization of cellulases and beta-glucosidases". The Biochemical Journal. 177 (1): 9-19. doi:10.1042/ ... The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ...
Galactosidases *Alpha. *Beta. *alpha-Mannosidase. *Glucuronidase *Klotho. *Hyaluronidase. *Pullulanase. *Glucosylceramidase * ...
Different temperatures optimize the activity of alpha or beta amylase, resulting in different mixtures of fermentable and ...
... (EC 3.2.1.101, exo-1,6-beta-mannanase, endo-alpha-1-,6-D-mannanase, endo-1,6-beta-mannanase, ... mannan endo-1,6-beta-mannosidase, 1,6-alpha-D-mannan mannanohydrolase) is an enzyme with systematic name 6-alpha-D-mannan ...
... beta-h-fructosidase, beta-fructosidase, invertin, sucrase, maxinvert L 1000, fructosylinvertase, alkaline invertase, acid ... invertase, and the systematic name: beta-fructofuranosidase. The resulting mixture of fructose and glucose is called inverted ...
In the beta form, the alcohol group is in the equatorial position, whereas in the alpha form, the alcohol group is in the axial ... The hydrolysis of lactose to glucose and galactose is catalyzed by the enzymes lactase and β-galactosidase. The latter is ... In the cyclic form there are two anomers, named alpha and beta, since the transition from the open-chain form to the cyclic ...
... they form a functional β-galactosidase. The presence of an active β-galactosidase may be detected when cells are grown in ... It has also been reported in at least 30 species of Proteobacteria distributed in the classes alpha, beta, gamma and epsilon. ... Reporter genes can be used as markers, such as the lacZ gene which codes for β-galactosidase used in blue-white screening. This ... Successful ligation therefore disrupts the lacZα gene, and no functional β-galactosidase can form, resulting in white colonies ...
Modeling β-galactosidase and butyrylcholinesterase". Biochimica et Biophysica Acta (BBA) - General Subjects. 1770 (5): 733-746 ...
Mutations in TGFBI which encodes transforming growth factor beta induced cause several forms of corneal dystrophies including ... males and asymptomatic female carriers of an X-linked systemic metabolic disease caused by a deficiency of α-galactosidase, ...
One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved ...
... β-galactosidase.[78] Significant results have been obtained by some companies to extend mouse lifespan focusing on senescent ... "Age-Related Loss of Innate Immune Antimicrobial Function Of Dermal Fat Is Mediated By Transforming Growth Factor Beta" ... "Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young ...
Other genetically modified pigs have had alpha galactosidase transferase knocked out and fortified with hCD46 and the hTM ... "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants". The EMBO Journal. 6 (13): ... "Transgenic pigs designed to express human α-galactosidase to avoid humoral xenograft rejection". J Appl Genet. 54: 293-303. ...
Beta-galactosidaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,O31341,O31341_BACCI Beta-galactosidase OS=Bacillus circulans GN=bgaC PE=1 SV=1 ...
... and a complete beta-galactosidase reporter system. Fast, simple assay for beta-galactosidase activity. ... Colorimetric and chemiluminescent assays for beta galactosidase, LacZ vectors, ... Beta-Galactosidase Assay & LacZ Vectors. The beta-galactosidase assay is commonly used as a reporter, to monitor transfection ... Complete Beta-Gal Reporter System. The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta- ...
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase ...
Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227 ... Caption: Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. ... Keywords: art, artwork, beta, biochemical, biochemistry, biological, biology, black background, compound, enzyme, galactosidase ...
Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic ... Keywords: artwork, b-gal, beta galactosidase, beta-gal, beta-galactosidase, biochemical, biochemistry, cut out, cut outs, cut- ... Caption: Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their ...
High-throughput beta-galactosidase assay for bacterial cell-based reporter systems. Biotechniques. 2004 Mar;36(3):410-5. PubMed ... Uses of lacZ to study gene function: evaluation of beta-galactosidase assays employed in the yeast two-hybrid system. Anal ... Ullmann A. Complementation in beta-galactosidase: from protein structure to genetic engineering. Bioessays. 1992 Mar;14(3):201- ... Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the ...
This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside ... Product Description for Beta-galactosidase. Mouse anti Human Beta-galactosidase OTI2F6.. Presentation: Purified. Product is ... This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside ... Beta-galactosidase (transcript variant 2). Not available. Human > 95 % Preparation: .. Purity Detail: >95% as determined by SDS ...
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C ... Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C ... Beta-Galactosidase, MBP, VSV) > Beta-Galactosidase (E. Coli) Protein for Western Blot (Western control) A-Z Product Search ... Beta-Galactosidase (E. Coli) Protein for Western Blot. Catalog#. Product Description. Product Type. Antibody Type. Size. Price ...
Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Determinants Of The 2 X-Gene Products Of Bovine ... Reference : Expression In Bacteria Of Beta-Galactosidase Fusion Proteins Carrying Antigenic Deter.... ...
... detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into ... strong,Rabbit anti beta-galactosidase polyclonal antibody,/strong, ... GO:0009341 beta-galactosidase complex Regulatory. For research purposes only. Applications of Beta Galactosidase antibody. ... Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the ...
... beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg ... microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI. Home , Preview ... microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI ... microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI ...
Description of Beta Galactosidase , Details of Beta Galactosidase , Article on Beta Galactosidase , Essay on Beta Galactosidase ... Learn more about Beta Galactosidase , Meaning of Beta Galactosidase , ... A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... β-galactosidase synthesis stops when glucose levels are sufficient.. Beta-galactosidase has many homologues based on similar ...
beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), ... The assay has greater practical utility and demonstrated significant differences in the perfusate beta-galactosidase between ... The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to ... Optimisation of an enzymatic method for beta-galactosidase. McGuire JBJ., James TJ., Imber CJ., St Peter SD., Friend PJ., ...
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and ... New heat shock puffs and betagalactosidase activity resulting from transformation of Drosophila with an hsp70‐lacZ hybrid gene ... Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression ... Lac repressor can be fused to betagalactosidase. Nature 249:561‐563. ...
Ser-796 of Beta-Galactosidase (E. coli) Plays a Key Role in Maintaining an Optimum Balance between the Opened and Closed ... Some enzymes in this group hydrolyze alpha-L-arabinosides; some animal enzymes also hydrolyze beta-D-fucosides and beta-D- ... Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D- galactosides. ...
A list of US medications equivalent to Beta-Galactosidase (Penicillium) is available on the Drugs.com website. ... Beta-Galactosidase (Penicillium) is a medicine available in a number of countries worldwide. ... Ingredient matches for Beta-Galactosidase (Penicillium). Tilactase. Beta-Galactosidase (Penicillium) (JAN) is also known as ... Beta-Galactosidase (Penicillium). Beta-Galactosidase (Penicillium) may be available in the countries listed below. ...
Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.. J. Mol. Biol. 343 1281-92 2004 ... This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. It has a jelly roll fold [ ... Beta-galactosidase jelly roll domain (IPR025300). Short name: BetaGal_jelly_roll_dom ...
... Martin Kennedy mkennedy at chmeds.ac.nz Tue Oct 5 21:23:18 EST 1993 *Previous message: ... with betagalactosidase constructs? ,, I am interested in staining the cells alive by adding Xgal to the culture ,, media ... Previous message: beta galactosidase in cultured cells *Next message: beta galactosidase in cultured cells ...
... Justin Kemp justin.news.invalid at web2news.net Wed Feb 26 23:31:21 EST 2003 *Previous ... I have been told that beta-galactosidase may be suitable. Can anyone tell me if it is indeed devoid of carbohydrate? Any help ...
Posted in Medical HacksTagged adenovirus, beta-galactosidase, biohacking, enzyme, genetic engineering, lactase, lactose ... The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or ...
The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the ... Suicide gene therapy using E. coli beta-galactosidase.. Farquhar D1, Pan BF, Sakurai M, Ghosh A, Mullen CA, Nelson JA. ... Daun02 was a good substrate for beta-gal. By comparison, gal-DCN4 was a poor substrate. Except for PC3, the beta-gal-transduced ... O-beta- D-galactopyranosyl)butyl]daunorubicin (gal-DNC4) were investigated. The prodrugs were evaluated as substrates for beta- ...
BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542566.2 → XP_011540868.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542569.2 → XP_011540871.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542570.2 → XP_011540872.1 beta-galactosidase-1-like protein 3 ... GanA; Beta-galactosidase GanA [Carbohydrate transport and metabolism]. * XM_011542572.1 → XP_011540874.1 beta-galactosidase-1- ...
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProt ...
Chicken polyclonal beta Galactosidase antibody. Validated in WB and tested in Escherichia coli. Immunogen corresponding to ... Anti-beta Galactosidase antibody (ab106567) at 1 µg/ml. Lane 1 : beta Galactosidase at 0.005 µg. Lane 2 : beta Galactosidase at ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... 17 amino acid peptide from near the N terminus of beta Galactosidase from E. coli (NP_752394). ...
β-galactosidase is found in plants, animals and microorganisms. In Beta-Galactosidase: Properties, Structure and Functions, the ... Beta-Galactosidase: Properties, Structure and Functions. Eloy Kras (Editor). Series: Cell Biology Research Progress. BISAC: ... Home / Shop / Books / Science and Technology / Life Sciences / Beta-Galactosidase: Properties, Structure and Functions. ... β-galactosidase is an enzyme responsible for catalyzing the hydrolysis of the lactose β-1,4 linkage into α-D-glucose and β-D- ...
Rabbit polyclonal GLB1/Beta-galactosidase antibody. Validated in IHC and tested in Mouse, Rat, Human. Cited in 5 publication(s ... Primary - Rabbit Anti-GLB1/Beta-galactosidase antibody (ab203749) IHC-P Protein - Recombinant Human GLB1/Beta-galactosidase ... Anti-GLB1/Beta-galactosidase antibody. See all GLB1/Beta-galactosidase primary antibodies. ... Isoform 2 has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic ...
Beta-galactosidaseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... sp,Q8X685,BGAL_ECO57 Beta-galactosidase OS=Escherichia coli O157:H7 OX=83334 GN=lacZ PE=3 SV=1 ... Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation. ,p>Manual validated ... IPR036156. Beta-gal/glucu_dom_sf. IPR011013. Gal_mutarotase_sf_dom. IPR008979. Galactose-bd-like_sf. IPR014718. GH-type_carb-bd ...
Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal ... Galactosidase Assay Reagent is a component of the Mammalian - ... Mammalian beta-Galactosidase Assay Kit. beta-Galactosidase ... The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which ... Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and- ...
Protein target information for Beta-galactosidase (Sinorhizobium meliloti). Find diseases associated with this biological ...
... "beta-Galactosidase" by people in Harvard Catalyst Profiles by year, and whether "beta-Galactosidase" was a major or minor topic ... non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1 ... "beta-Galactosidase" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "beta-Galactosidase" by people in Profiles. ...
Invitrogen Anti-beta Galactosidase Monoclonal (DC1 4C7), Catalog # MA1-152. Tested in Western Blot (WB), Immunofluorescence (IF ... Cite beta Galactosidase Monoclonal Antibody (DC1 4C7). The following antibody was used in this experiment: beta Galactosidase ... Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. Beta Galactosidase can be ... coli beta Galactosidase at ~110 kDa. This antibody also detects E. coli beta Galactosidase in pCMV-lacZ transfected HeLa cells. ...
The Beta-Galactosidase Staining Kit makes it easy to determine the transfection efficiency of mammalian cells using co- ... Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit.. One µg of a 119 kDa subunit of β-galactosidase was ... The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lacZ ... The gene product of lacZ, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily ...
... beta-galactosidase explanation free. What is beta-galactosidase? Meaning of beta-galactosidase medical term. What does beta- ... Looking for online definition of beta-galactosidase in the Medical Dictionary? ... medical-dictionary.thefreedictionary.com/beta-galactosidase,beta-galactosidase,/a,. *Facebook ... A gene on chromosome 3p21.33 that encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyses the terminal beta-galactose ...
The isolated enzyme forms accounted for 83%, 8%, and 9% of the total beta-galactosidase activity, respectively … ... The enzyme beta-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using ... The beta-galactosidase forms were heat-stable up to about 60 degrees C. The Km values for lactose ranged from 85 mM to 125 mM, ... beta-Galactosidase From Aspergillus Niger. Separation and Characterization of Three Multiple Forms Eur J Biochem. 1979 Oct 15; ...
These protocols are used to screen bacterial plates for beta-galactosidase activity using X-Gal (aka blue white screening). ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Beta-galactosidase_Screen&oldid=537279" ...
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ... Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast ...
beta-Galactosidase (Lactaid). 7/2011. GM1 gangliosidosis and Morquio B disease: an update on genetic alterations and clinical ... Neuraminidase deficiency with beta-galactosidase deficiencyIBA 06/2014 - 06/2002. 6. 5-deoxy-5-phosphonomethyladenosine ...
beta-Galactosidase (Lactaid). 9/2008. Molecular cloning, characterization and regulation of a peroxiredoxin gene from ...
  • A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase. (openwetware.org)
  • This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates. (acris-antibodies.com)
  • beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. (bio-rad-antibodies.com)
  • This plasmid contains a c-terminal Beta Galactosidase reporter tag that can be fused to a gene of interest to allow protein detection. (oxfordgenetics.com)
  • Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. (bio-rad-antibodies.com)
  • If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here. (openwetware.org)
  • CONCLUSIONS: The assay has greater practical utility and demonstrated significant differences in the perfusate beta-galactosidase between cold-stored and warm-perfused livers in a porcine model of transplantation. (ox.ac.uk)
  • BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. (ox.ac.uk)
  • beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. (ox.ac.uk)
  • A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase. (openwetware.org)
  • Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. (openwetware.org)
  • Altered expression of β-galactosidase-1-like protein 3 (Glb1l3) in the retinal pigment epithelium (RPE)-specific 65-kDa protein knock-out mouse model of Leber's congenital amaurosis. (nih.gov)
  • The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification. (thermofisher.com)
  • The kit can also be used to determine the efficiency of direct delivery of β-galactosidase by the Chariot™ Protein Delivery Reagent (Figure 1). (activemotif.com)
  • One µg of a 119 kDa subunit of β-galactosidase was complexed with Chariot™ Protein Delivery Reagent for 30 minutes and delivered into HeLa cells. (activemotif.com)
  • Beta-galactosidase - protein Name = caption = β galactosidase from Penicillum sp. (academic.ru)
  • We conclude that the beta-galactosidase precursor is functionally identical to the mature lysosomal form of the enzyme and serves as an excellent enzyme source for investigation of structure-function relationships in the protein. (ubc.ca)
  • Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. (bio-rad-antibodies.com)
  • Galactosialidosis is an autosomal recessive lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase secondary to a defect in the cathepsin A protein. (testcatalog.org)
  • Western blot analysis of Beta-gal expression in total protein extracts from transfected cells and transgenic mice expressing beta-gal protein. (euromabnet.com)
  • GLB1 (Galactosidase Beta 1) is a Protein Coding gene. (genecards.org)
  • β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. (wikipedia.org)
  • Many CTSA mutations disrupt the protein structure of cathepsin A, impairing its ability to join with neuraminidase 1 and beta-galactosidase or elastin binding protein. (medlineplus.gov)
  • Most mutations in the CTSA gene cause a lack of functional cathepsin A and a loss of neuraminidase 1, beta-galactosidase, and elastin binding protein. (medlineplus.gov)
  • New mutations in the PPBG gene lead to loss of PPCA protein which affects the level of the beta-galactosidase/neuraminidase complex and the EBP-receptor. (medlineplus.gov)
  • The enzyme beta-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using molecular sieving, ion-exchange, an hydrophobic chromatography. (nih.gov)
  • Beta Galactosidase (Beta-Gal) Activity Assay Kit (Fluorometric) Beta Galactosidase (Beta-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the Beta-galactosides into monosacchanides. (assaygenie.com)
  • Beta Galactosidase (Beta-Gal) Inhibitor Screening Kit (Fluorometric) Beta Galactosidase (Beta-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the. (assaygenie.com)
  • The food enzyme β‐d‐galactosidase galactohydrolase (EC 3.2.1.23) is produced with Bacillus sp. (europa.eu)
  • Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones. (thermofisher.com)
  • Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. (abnova.com)
  • Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN). (wikipedia.org)
  • B-galactosidase can be used to track the efficiency of bacterial transformation with a recombinant plasmid in a process called Blue/White Color Screening. (wikipedia.org)
  • Therefore, when the bacteria is transformed with the recombinant plasmid B-galactosidase is inactive and the colonies appear white, but when bacteria are transformed with the original plasmid, lacking the target gene, B-galactosidase is active and the colonies appear blue. (wikipedia.org)
  • The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or lactase, and use it to infect the cells of his small intestine. (hackaday.com)
  • β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (infospaze.com)
  • A few are evolved beta-galactosidase (EBG), beta-glucosidase , 6-phospho-beta-galactosidase, beta-mannosidase, and lactase-phlorizin hydrolase. (infospaze.com)
  • Many adult humans lack the lactase enzyme, which has the same function of beta-gal, so they are not able to properly digest dairy products. (infospaze.com)
  • In this kit, beta-galactosidase (the product of the LacZ gene) catalyzes the hydrolysis of X-gal to produce a blue color that is easily visualized. (clontech.com)
  • The gene product of lac Z, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily visualized. (activemotif.com)
  • Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. (uzh.ch)
  • We show that the stereochemical course of enzymic hydrolysis involves the retention of the beta-configuration at the anomeric centre, suggesting a double-displacement mechanism. (ubc.ca)
  • Beta-galactosidase is a lysosomal enzyme responsible for catalyzing the hydrolysis of gangliosides. (testcatalog.org)
  • Galactosidases are enzymes (glycoside hydrolases) that catalyze the hydrolysis of galactosides into monosaccharides. (wikipedia.org)
  • If the galactoside is classified as an alpha-galactoside, the enzyme is called alpha-galactosidase, and is responsible for catalyzing the hydrolysis of substrates that contain α-galactosidic residues, such as glycosphingolipids or glycoproteins. (wikipedia.org)
  • The prodrugs were evaluated as substrates for beta-gal. (nih.gov)
  • The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others. (thermofisher.com)
  • The LacZ gene encodes the enzyme Beta-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ. (assaygenie.com)
  • Beta-galactosidase is the product of the LacZ gene. (clontech.com)
  • We offer three beta-gal mammalian reporter vectors containing the LacZ gene. (clontech.com)
  • A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase. (openwetware.org)
  • In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity. (semanticscholar.org)
  • The P10 promoter directed the synthesis of beta-galactosidase, whereas the polyhedrin promoter controlled the synthesis of foreign gene products. (asm.org)
  • Download file to see previous pages Apart from induction, synthesis rate is determined by catabolite repression, whereby it slows down the synthesis of beta-galactosidase especially in the presence of a better carbon (and energy) source, such as glucose. (studentshare.org)
  • 66586-1-Ig targets Beta galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with Human samples. (ptglab.com)
  • These two genes recombined with wild-type virus genome to yield recombinants which were polyhedrin negative, produced the foreign gene product, and formed blue plaques when beta-galactosidase indicator was present in the agarose overlay. (asm.org)
  • Portanto, o bioprocesso de imobilização de β-galactosidase de Bacillus circulans em macroesfera de quitosana pode ser considerado um potencial catalisador para produção industrial de lactosacarose. (ufrgs.br)
  • Therefore, the bioprocess of β-galactosidase from Bacillus circulans immobilization on macrospheres of chitosan can be considered a potential catalyst for industrial. (ufrgs.br)
  • Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (europa.eu)
  • Alpha-galactosidase - is a glycoside hydrolase enzyme that hydrolyses the terminal alpha galactosyl moieties from glycolipids and glycoproteins. (academic.ru)
  • B-galactosidase forms the basis of lac z operon in bacteria which can be used to control gene expression. (wikipedia.org)
  • Isoform 1: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. (genecards.org)
  • Glycoside hydrolase, family 2 / Beta galactosidase small chain/ domain 5 / Domain of unknown function(DUF4981) / Glycosyl hydrolases family 2 acid/base catalyst. (pdbj.org)
  • FDG Beta-Galactosidase Assay Kit FDG Beta-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active. (assaygenie.com)
  • ONPG Galactosidase Assay Kit ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active. (assaygenie.com)
  • Beta-galactosidase enzyme is deficient in the following conditions: GM1 gangliosidosis, Morquio syndrome B, and galactosialidosis. (mayocliniclabs.com)
  • In this method of screening, the host E. coli strain carries the lacZ deletion mutant (lacZΔM15) which contains the ω-peptide, while the plasmids used carry the lacZα sequence which encodes the first 59 residues of β-galactosidase, the α-peptide. (wikipedia.org)
  • Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. (uzh.ch)
  • beta-Galactosidase From Aspergillus Niger. (nih.gov)
  • beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. (bio-rad-antibodies.com)
  • Li X, Jiang Y, Chong S, Walt DR. Bottom-up single-molecule strategy for understanding subunit function of tetrameric ß-galactosidase. (harvard.edu)
  • 1. A process for producing a galactosyl.beta.1,3glycal disaccharide that comprises admixing (i) a galactoside donor molecule, (ii) glucal, 6-O--C.sub.1 -C.sub.6 acyl glucal or6-O--C.sub.1 -C.sub.6 acyl galactal as acceptor and (iii) a catalytic amount of .beta. (patentgenius.com)
  • Chinese hamster ovary cell clones permanently transfected with the cDNA for human lysosomal beta-galactosidase secrete the enzyme precursor into the cell medium, from which it is purified to apparent homogeneity in a single step by affinity chromatography. (ubc.ca)
  • A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes. (abnova.com)
  • Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml monclonal andibody monoclonal antobodies are directed against a specific epitope. (antibody-antibodies.com)
  • Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml immunoglobuline1 The Mouse Monoclonal to beta-Galactosidase, Clone BG-02, Isotype IgG1Application WB, ICC Concentration 1 mg ml is very stable with a high affinity. (antibody-antibodies.com)
  • B-galactosidase is made up of 4 identical polypeptide chains, i.e. it has 4 identical subunits. (wikipedia.org)
  • beta-galactosidase rat anti E.coli, clone 3A9A. (euromabnet.com)
  • Anti-beta-gal (Clone 3A9A) immunohistochemistry in mouse paraffin tissues. (euromabnet.com)