Defensins
alpha-Defensins
Encyclopedias as Topic
Urogenital System
All the organs involved in reproduction and the formation and release of URINE. It includes the kidneys, ureters, BLADDER; URETHRA, and the organs of reproduction - ovaries, UTERUS; FALLOPIAN TUBES; VAGINA; and CLITORIS in women and the testes; SEMINAL VESICLES; PROSTATE; seminal ducts; and PENIS in men.
Antimicrobial Cationic Peptides
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
Disulfides
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Acne Vulgaris
Blood-Nerve Barrier
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Neutrophils
Solutions
beta 2-Glycoprotein I
A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.
Antibodies
Antiphospholipid Syndrome
The presence of antibodies directed against phospholipids (ANTIBODIES, ANTIPHOSPHOLIPID). The condition is associated with a variety of diseases, notably systemic lupus erythematosus and other connective tissue diseases, thrombopenia, and arterial or venous thromboses. In pregnancy it can cause abortion. Of the phospholipids, the cardiolipins show markedly elevated levels of anticardiolipin antibodies (ANTIBODIES, ANTICARDIOLIPIN). Present also are high levels of lupus anticoagulant (LUPUS COAGULATION INHIBITOR).
Antibody Specificity
Antibodies, Anticardiolipin
Antiphospholipid antibodies found in association with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; and in a variety of other diseases as well as in healthy individuals. The antibodies are detected by solid-phase IMMUNOASSAY employing the purified phospholipid antigen CARDIOLIPIN.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Cross Reactions
Cloning, Molecular
Magnetic Resonance Spectroscopy
Salmonella typhimurium
Intestine, Small
Variant Surface Glycoproteins, Trypanosoma
Glycoproteins attached to the surface coat of the trypanosome. Many of these glycoproteins show amino acid sequence diversity expressed as antigenic variations. This continuous development of antigenically distinct variants in the course of infection ensures that some trypanosomes always survive the development of immune response to propagate the infection.
Salmonella
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
Production of beta-defensin antimicrobial peptides by the oral mucosa and salivary glands. (1/685)
beta-Defensins are cationic peptides with broad-spectrum antimicrobial activity that are produced by epithelia at mucosal surfaces. Two human beta-defensins, HBD-1 and HBD-2, were discovered in 1995 and 1997, respectively. However, little is known about the expression of HBD-1 or HBD-2 in tissues of the oral cavity and whether these proteins are secreted. In this study, we characterized the expression of HBD-1 and HBD-2 mRNAs within the major salivary glands, tongue, gingiva, and buccal mucosa and detected beta-defensin peptides in salivary secretions. Defensin mRNA expression was quantitated by RNase protection assays. HBD-1 mRNA expression was detected in the gingiva, parotid gland, buccal mucosa, and tongue. Expression of HBD-2 mRNA was detected only in the gingival mucosa and was most abundant in tissues with associated inflammation. To test whether beta-defensin expression was inducible, gingival keratinocyte cell cultures were treated with interleukin-1beta (IL-1beta) or bacterial lipopolysaccharide (LPS) for 24 h. HBD-2 expression increased approximately 16-fold with IL-1beta treatment and approximately 5-fold in the presence of LPS. Western immunoblotting, liquid chromatography, and mass spectrometry were used to identify the HBD-1 and HBD-2 peptides in human saliva. Human beta-defensins are expressed in oral tissues, and the proteins are secreted in saliva; HBD-1 expression was constitutive, while HBD-2 expression was induced by IL-1beta and LPS. Human beta-defensins may play an important role in the innate defenses against oral microorganisms. (+info)Efficient killing of inhaled bacteria in DeltaF508 mice: role of airway surface liquid composition. (2/685)
Cystic fibrosis mice have been generated by gene targeting but show little lung disease without repeated exposure to bacteria. We asked if murine mucosal defenses and airway surface liquid (ASL) Cl(-) were altered by the DeltaF508 cystic fibrosis transmembrane conductance regulator mutation. Naive DeltaF508 -/- and +/- mice showed no pulmonary inflammation and after inhaled Pseudomonas aeruginosa had similar inflammatory responses and bacterial clearance rates. We therefore investigated components of the innate immune system. Bronchoalveolar lavage fluid from mice killed Escherichia coli, and the microbicidal activity was inhibited by NaCl. Because beta-defensins are salt-sensitive epithelial products, we looked for pulmonary beta-defensin expression. A mouse homolog of human beta-defensin-1 (termed "MBD-1") was identified; the mRNA was expressed in the lung. Using a radiotracer technique, ASL volume and Cl(-) concentration ([Cl(-)]) were measured in cultured tracheal epithelia from normal and DeltaF508 -/- mice. The estimated ASL volume was similar for both groups. There were no differences in ASL [Cl(-)] in DeltaF508 -/- and normal mice (13.8 +/- 2.6 vs. 17.8 +/- 5.6 meq/l). Because ASL [Cl(-)] is low in normal and mutant mice, salt-sensitive antimicrobial factors, including MBD-1, may be normally active. (+info)Molecular cloning and characterization of rat genes encoding homologues of human beta-defensins. (3/685)
beta-Defensins are cationic peptides with broad-spectrum antimicrobial activity that may play a role in mucosal defenses of several organs. They have been isolated in several species, and in humans, two beta-defensins have been identified. Here, we report the identification of two genes encoding beta-defensin homologues in the rat. Partial cDNAs were found by searching the expressed-sequence-tag database, and primers were designed to generate full-length mRNA coding sequences. One gene was highly similar to the human beta-defensin-1 (HBD-1) gene and mouse beta-defensin-1 gene at both the nucleic acid and amino acid levels and was termed rat beta-defensin-1 (RBD-1). The other gene, named RBD-2, was homologous to the HBD-2 and bovine tracheal antimicrobial peptide (TAP) genes. The predicted prepropeptides were strongly cationic, were 69 and 63 residues in length for RBD-1 and RBD-2, respectively, and contained the six-cysteine motif characteristic of beta-defensins. The beta-defensin genes mapped closely on rat chromosome 16 and were closely linked to the alpha-defensins genes, suggesting that they are part of a gene cluster, similar to the organization reported for humans. Northern blot analysis showed that both RBD-1 and RBD-2 mRNA transcripts were approximately 0.5 kb in length; RBD-1 mRNA was abundantly transcribed in the rat kidney, while RBD-2 was prevalent in the lung. Reverse transcription-PCR indicated that RBD-1 and RBD-2 mRNAs were distributed in a variety of other tissues. In the lung, RBD-1 mRNA expression localized to the tracheal epithelium while RBD-2 was expressed in alveolar type II cells. In conclusion, we characterized two novel beta-defensin homologues in the rat. The rat may be a useful model to investigate the function and contribution of beta-defensins to host defense in the lung, kidney, and other tissues. (+info)Human beta-defensin-1 mRNA is transcribed in tympanic membrane and adjacent auditory canal epithelium. (4/685)
The external auditory canal is less susceptible to infections than the sensitive middle-ear cavity. Since recent research has provided insight to the production of potent antimicrobial peptides from various surface epithelia, we wanted to investigate whether protection of the external auditory canal in part could be explained by the production of human beta-defensin-1 (HBD-1). This particular peptide is known to be constitutively expressed in various surface epithelia, such as airway, skin, and urogenital tissues. By reverse transcriptase PCR we demonstrate HBD-1 mRNA in the pars tensa and pars flaccida of the tympanic membrane and in the meatal skin. In situ hybridization studies localized the HBD-1 mRNA to the epidermal layer of these tissues. The HBD-1 transcripts were also evident in the sebaceous glands and in hair follicles of the meatal skin. In contrast, HBD-1 mRNA was not detected in the tympanal epithelium of the eardrum. The widespread presence of mRNA encoding for this broad-spectrum antimicrobial peptide in the meatal skin and tympanic membrane suggests that HBD-1 participates in the innate antimicrobial defense of the external auditory canal and middle-ear cavity. (+info)Cloning and expression of bovine neutrophil beta-defensins. Biosynthetic profile during neutrophilic maturation and localization of mature peptide to novel cytoplasmic dense granules. (5/685)
beta-Defensins are microbicidal peptides implicated in host defense functions of phagocytic leukocytes and certain surface epithelial cells. Here we investigated the genetic structures and cellular expression of BNBD-4, -12, and -13, three prototypic bovine neutrophil beta-defensins. Characterization of the corresponding cDNAs indicated that BNBD-4 (41 residues) derives from a 63-amino acid prepropeptide and that BNBD-12 (38 residues) and BNBD-13 (42 residues) derive from a common 60-amino acid precursor (BNBD-12/13). The peptides were found to be encoded by two-exon genes that are closely related to bovine epithelial beta-defensin genes. BNBD-4 and BNBD-12/13 mRNAs were most abundant in bone marrow, but were expressed differentially in certain non-myeloid tissues. In situ hybridization and immunohistochemical studies demonstrated that BNBD-4 synthesis is completed early in myelopoiesis. BNBD-12 was localized exclusively to the novel dense granules, organelles that also contain precursors of cathelicidins, antimicrobial peptides that undergo proteolytic processing during phagocytosis. In contrast to cathelicidins, Western blot analyses revealed that mature beta-defensins are the predominant organellar form in myeloid cells. Stimulation of neutrophils with phorbol myristate acetate induced secretion of BNBD-12, indicating that it is co-secreted with pro-cathelicidins. The exocytosis of BNBD-12 by activated neutrophils reveals different mobilization pathways for myeloid alpha- and beta-defensins. (+info)Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. (6/685)
Defensins contribute to host defense by disrupting the cytoplasmic membrane of microorganisms. This report shows that human beta-defensins are also chemotactic for immature dendritic cells and memory T cells. Human beta-defensin was selectively chemotactic for cells stably transfected to express human CCR6, a chemokine receptor preferentially expressed by immature dendritic cells and memory T cells. The beta-defensin-induced chemotaxis was sensitive to pertussis toxin and inhibited by antibodies to CCR6. The binding of iodinated LARC, the chemokine ligand for CCR6, to CCR6-transfected cells was competitively displaced by beta-defensin. Thus, beta-defensins may promote adaptive immune responses by recruiting dendritic and T cells to the site of microbial invasion through interaction with CCR6. (+info)Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. (7/685)
The intestinal epithelium forms a physical barrier to limit access of enteric microbes to the host and contributes to innate host defense by producing effector molecules against luminal microbes. To further define the role of the intestinal epithelium in antimicrobial host defense, we analyzed the expression, regulation, and production of two antimicrobial peptides, human defensins hBD-1 and hBD-2, by human intestinal epithelial cells in vitro and in vivo. The human colon epithelial cell lines HT-29 and Caco-2 constitutively express hBD-1 mRNA and protein but not hBD-2. However, hBD-2 expression is rapidly induced by IL-1alpha stimulation or infection of those cells with enteroinvasive bacteria. Moreover, hBD-2 functions as a NF-kappaB target gene in the intestinal epithelium as blocking NF-kappaB activation inhibits the up-regulated expression of hBD-2 in response to IL-1alpha stimulation or bacterial infection. Caco-2 cells produce two hBD-1 isoforms and a hBD-2 peptide larger in size than previously described hBD-2 isoforms. Paralleling the in vitro findings, human fetal intestinal xenografts constitutively express hBD-1, but not hBD-2, and hBD-2 expression, but not hBD-1, is up-regulated in xenografts infected intraluminally with Salmonella. hBD-1 is expressed by the epithelium of normal human colon and small intestine, with a similar pattern of expression in inflamed colon. In contrast, there is little hBD-2 expression by the epithelium of normal colon, but abundant hBD-2 expression by the epithelium of inflamed colon. hBD-1 and hBD-2 may be integral components of epithelial innate immunity in the intestine, with each occupying a distinct functional niche in intestinal mucosal defense. (+info)Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. (8/685)
Human gingival epithelial cells (HGE) express two antimicrobial peptides of the beta-defensin family, human beta-defensin 1 (hBD-1) and hBD-2, as well as cytokines and chemokines that contribute to innate immunity. In the present study, the expression and transcriptional regulation of hBD-2 was examined. HBD-2 mRNA was induced by cell wall extract of Fusobacterium nucleatum, an oral commensal microorganism, but not by that of Porphyromonas gingivalis, a periodontal pathogen. HBD-2 mRNA was also induced by the proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) and phorbol myristate acetate (PMA), an epithelial cell activator. HBD-2 mRNA was also expressed in 14 of 15 noninflamed gingival tissue samples. HBD-2 peptide was detected by immunofluorescence in HGE stimulated with F. nucleatum cell wall, consistent with induction of the mRNA by this stimulant. Kinetic analysis indicates involvement of multiple distinct signaling pathways in the regulation of hBD-2 mRNA; TNF-alpha and F. nucleatum cell wall induced hBD-2 mRNA rapidly (2 to 4 h), while PMA stimulation was slower ( approximately 10 h). In contrast, each stimulant induced interleukin 8 (IL-8) within 1 h. The role of TNF-alpha as an intermediary in F. nucleatum signaling was ruled out by addition of anti-TNF-alpha that did not inhibit hBD-2 induction. However, inhibitor studies show that F. nucleatum stimulation of hBD-2 mRNA requires both new gene transcription and new protein synthesis. Bacterial lipopolysaccharides isolated from Escherichia coli and F. nucleatum were poor stimulants of hBD-2, although they up-regulated IL-8 mRNA. Collectively, our findings show inducible expression of hBD-2 mRNA via multiple pathways in HGE in a pattern that is distinct from that of IL-8 expression. We suggest that different aspects of innate immune responses are differentially regulated and that commensal organisms have a role in stimulating mucosal epithelial cells in maintaining the barrier that contributes to homeostasis and host defense. (+info)
Gentaur Molecular :Ray Biotech \ Recombinant Human beta Defensin-2 \ 228-10109-1
Gentaur Molecular :Ray Biotech \ Recombinant Human beta Defensin-3 \ 228-10110-3
Antimicrobial Peptide Coating of Dental Implants: Biocompatibility Assessment of Recombinant Human Beta Defensin-2 for Human...
Beta defensin - Wikipedia
Detection of human beta defensin-1 and −2 by RT-competitive multiplex PCR | SpringerLink
Rapid sequence divergence in mammalian beta-defensins by adaptive evolution<...
Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells.
Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the...
The Novel Human Beta-defensin 114 Regulates Lipopolysaccharide(LPS)-mediated Inflammation and Protects Sperm from Motility Loss...
RCSB PDB
- 1BNB: SOLUTION STRUCTURE OF BOVINE NEUTROPHIL BETA-DEFENSIN 12: THE PEPTIDE FOLD OF THE BETA-DEFENSINS IS...
Epithelial Antimicrobial Peptides: Guardian of the Oral Cavity
Suppression of pulmonary innate host defense in smokers | Thorax
Southern California Conferences for Undergraduate Research - Southern California Conferences for Undergraduate Research:...
Effects of Pimecrolimus on Skin Biopsy Ex-plants From Patients With Atopic Dermatitis - Full Text View - ClinicalTrials.gov
Polymers | Free Full-Text | Synthesis and Gas Transport Properties of Hyperbranched Polyimide-Silica Hybrid/Composite Membranes
Polymers | Free Full-Text | Synthesis and Gas Transport Properties of Hyperbranched Polyimide-Silica Hybrid/Composite Membranes
beta-Defensin 4/2 ELISA Kits: Novus Biologicals
ActoFactor™ Recombinant Human Interleukin 8 | Creative Bioarray
DISEASES - GPR29
Beta-defensin13 - Creative Peptides
Beta-defensin 2 - Creative Peptides
OriGene - DEFB119 (NM 153323) cDNA Clone
DEFB126 Gene - GeneCards | DB126 Protein | DB126 Antibody
DEFB107A Gene - GeneCards | D107A Protein | D107A Antibody
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Canned Dog Food - Pork & Venison
Frontiers | Conceptual perspectives: Bacterial antimicrobial peptide induction as a novel strategy for symbiosis with the human...
History of eczema herpeticum is associated with the inability to induce human beta-defensin (HBD)-2, HBD-3 and cathelicidin in...
DEFB106A - Wikipedia
Human Gene DEFB107A (ENST00000335021.2)
Honey, Defensin-1 Effective Against Biofilm
Expression of beta-defensins pBD-1 and pBD-2 along the small tract of the pig: Lack of upregulation in vivo upon Salmonella...
Case Western Reserve University/University Hospitals Cleveland Medical Center - American Head & Neck Society
JCI -
Dual role of α-defensin-1 in anti-HIV-1 innate immunity
Apitherapy News: Honey, Royal Jelly Component Defensin-1 Boosts Wound Healing
Ageing and free-living daily physical activity effects on salivary beta-defensin 2 secretion<...
Human beta-defensin - hepaton
Malassezia Furfur Induces the Expression of beta-defensin-2 in Human Keratinocytes in a Protein Kinase C-dependent Manner -...
Cardiac Defensins--Natural Antibiotics of the Heart - WSAVA2005 - VIN
High-Dose Vitamin D and Antimicrobial Peptide Expression in Lung Failure - Gregory Martin
A novel antimicrobial peptide on the ocular surface shows decreased expression in inflammation and infection. - Semantic Scholar
In Lieu of Lecture: Stuff Teachers Say
Recombinant Human DEFB110 Protein, GST-tagged DEFB110-2524H - Creative BioMart
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Recombinant Human BD-1 (36 a.a.)
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Defb2 - mouse gene knockout kit via CRISPR - KN304451 | acris-antibodies.com
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Anti-Beta Defensin 1 Antibody
Search Articles | University of Toronto Libraries
Chromosome 20
DEFB118, DEFB119, DEFB126, DEFB127, DEFB129: Beta-defensin genes. *DLGAP4: Disks large-associated protein 4 ...
DEFB129
The protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found ... Beta-defensin 129 is a protein that in humans is encoded by the DEFB129 gene. Defensins are cysteine-rich cationic polypeptides ... "Entrez Gene: DEFB129 defensin, beta 129". CS1 maint: discouraged parameter (link) Gerhard DS, Wagner L, Feingold EA, et al. ( ... Kao CY, Chen Y, Zhao YH, Wu R (2003). "ORFeome-based search of airway epithelial cell-specific novel human [beta]-defensin ...
DEFB106A
... alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic ... Beta-defensin 106 is a protein that in humans is encoded by the DEFB106A gene. Defensins form a family of microbicidal and ... "Entrez Gene: DEFB106A defensin, beta 106A". CS1 maint: discouraged parameter (link) Xin A (Jan 2014). "Soluble fusion ... Kao CY, Chen Y, Zhao YH, Wu R (2003). "ORFeome-based search of airway epithelial cell-specific novel human [beta]-defensin ...
DEFB104A
... alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic ... Beta-defensin 104 is a protein that in humans is encoded by the DEFB104A gene. Defensins form a family of microbicidal and ... "Entrez Gene: DEFB104A defensin, beta 104A". CS1 maint: discouraged parameter (link) Patil AA, Cai Y, Sang Y, Blecha F, Zhang G ... Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical ...
Ocular immune system
TGF beta - important in maintaining immune privilege P.G. McMenamin, The distribution of immune cells in the uveal tract of the ... The latter, defensins, have a wide range of antimicrobial affects against bacteria, fungi, and viruses, as well as effects in ... 74(2): p. 293-9. Gottsch, J.D., et al., Defensin gene expression in the cornea. Curr Eye Res, 1998. 17(11): p. 1082-6. Oakes, J ... It has also been found that the presence of secreted defensins secreted by corneal keratocytes is correlated with cases of ...
DEFB103A
2002). "Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. ... Beta-defensin 103 is a protein that in humans is encoded by the DEFB103A gene. Defensins form a family of microbicidal and ... Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad ... "Entrez Gene: DEFB103A defensin, beta 103A". Candille SI, Kaelin CB, Cattanach BM, Yu B, Thompson DA, Nix MA, Kerns JA, Schmutz ...
C-C chemokine receptor type 6
1999). "Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6". Science. 286 (5439): 525-8. ... Linking antimicrobial and CC chemokine receptor-6-binding activities with human beta-defensins". J. Biol. Chem. 277 (40): 37647 ... V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity". Blood. 100 (1): 11-6. doi:10.1182/blood-2001-12-0196 ...
Virtual colony count
These studies have been expanded to include additional beta defensins, theta defensins, and the human cathelicidin LL-37 and ... HD5 and Beta-defensin 2 composed entirely of D-amino acids suggested differing mechanisms of defensin activity against Gram- ... Subsequently, mutated forms of some of those six defensins were studied by VCC. A conserved glycine in a beta bulge in HNP2 was ... This effect is often observed with beta lactams when assayed against beta lactamase producing bacteria. The inoculum effect was ...
CCL20
1999). "Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6". Science. 286 (5439): 525-528. ... 1997). "Cloning and characterization of exodus, a novel beta-chemokine". Blood. 89 (9): 3315-22. PMID 9129037. Baba M, Imai T, ... immature dendritic cells generated from monocytes in the presence of TGF-beta 1 express functional C-C chemokine receptor 6". J ...
Antimicrobial peptides
... avian defensins) and mammals (e.g. cathelicidins, alpha- and beta-defensins, regIII peptides) Research has increased in recent ... In fact human beta-defensin 2 have now been shown to kill the pathogenic fungi Candida albicans through interactions with ... Sawaki K, Mizukawa N, Yamaai T, Yoshimoto T, Nakano M, Sugahara T (2002). "High concentration of beta-defensin-2 in oral ... KIDA-TAKAOKA, SAORI (2014). "Surrounding cells affect the gene expression pattern of human beta-defensins in squamous cell ...
Airway basal cell
Other innate immune mediators include beta-defensin 2, and lipocalin-2; pro-inflammatory cytokines, interleukins IL6, and IL8; ...
Cathelicidin
"Epithelial cell-derived antibacterial peptides human beta-defensins and cathelicidin: multifunctional activities on mast cells ... The AMP family also includes the defensins. Whilst the defensins share common structural features, cathelicidin-related ... Additionally cathelicidins may also be small-sized molecules (12-18 residues) with beta-hairpin structures, stabilized by one ... 3 and cathelicidin Beta-1 Tasmanian Devil: Saha-CATH5 Salmonids: CATH1 and CATH2 Patients with rosacea have elevated levels of ...
Perdita Barran
She led an EPSRC platform grant to study the structure-activity relationships of Beta defensins. She works with Cait MacPhee, ...
DEFB105A
Alpha defensins, Beta defensins and Theta defensins. All beta-defensin genes are densely clustered in four to five syntenic ... Beta-defensin 105 is a protein that is encoded by the DEFB105A gene in humans. Defensins form a family of microbicidal and ... "Entrez Gene: DEFB105A defensin, beta 105A". CS1 maint: discouraged parameter (link) Patil AA, Cai Y, Sang Y, et al. (2006). " ... Semple CA, Rolfe M, Dorin JR (May 2003). "Duplication and selection in the evolution of primate beta-defensin genes". Genome ...
Sperm-associated antigen 11B
Some of the isoforms contain regions of similarity to beta-defensins, a family of antimicrobial peptides. The gene is located ... "Organization of the human gene encoding the epididymis-specific EP2 protein variants and its relationship to defensin genes". ... on chromosome 8p23 near the defensin gene cluster. Alternative splicing of this gene results in seven transcript variants ...
DEFA5
Zhao C, Wang I, Lehrer RI (November 1996). "Widespread expression of beta-defensin hBD-1 in human secretory glands and ... Defensin, alpha 5 (DEFA5) also known as human alpha defensin 5 (HD5) is a protein that in humans is encoded by the DEFA5 gene. ... "Entrez Gene: DEFA5 defensin, alpha 5, Paneth cell-specific". Jones DE, Bevins CL (January 1993). "Defensin-6 mRNA in human ... Several of the human alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, α-defensin- ...
DEFA6
Zhao C, Wang I, Lehrer RI (24 Nov 1996). "Widespread expression of beta-defensin hBD-1 in human secretory glands and epithelial ... Defensin, alpha 6 (DEFA6) also known as human alpha defensin 6 (HD6) is a human protein that is encoded by the DEFA6 gene. ... "Entrez Gene: DEFA6 defensin, alpha 6, Paneth cell-specific (Homo sapiens)". Jones DE, Bevins CL (January 1993). "Defensin-6 ... The alpha defensins are a family of microbicidal and cytotoxic peptides that defend the host against bacteria and viruses. HD6 ...
Earwax
Bøe R, Silvola J, Yang J, Moens U, McCray PB, Stenfors LE, Seljfelid R (September 1999). "Human beta-defensin-1 mRNA is ...
Thionin
Alpha- and beta- thionins are related to each other. The gamma thionins have a superficially similar structure but are an ... unrelated class of protein, now called plant defensins. The proteins are toxic to animal cells, presumably attacking the cell ...
Microbial symbiosis and immunity
As a result, the intestinal epithelial cells express chemokine ligand 20 (CCL20) and Beta defensin 3. CCL20 and Beta-defensin 3 ... S. epidermidis secretes a small molecule AMP which leads to increased expression of Human β-defensins. S. epidermidis also ...
Kynurenine
"Beta-defensin 1, aryl hydrocarbon receptor and plasma kynurenine in major depressive disorder: metabolomics-informed genomics ...
Vaginal epithelium
The epithelium further provides a barrier to microbes by the synthesis of antimicrobial peptides (beta-defensins and ...
Peptidoglycan recognition protein 2
... involvement in beta-defensin-1 expression". Infection and Immunity. 73 (11): 7133-41. doi:10.1128/IAI.73.11.7133-7141.2005. PMC ...
Tachystatin
... A is thought to have an antimicrobial activity similar to defensins. Fujitani N, Kawabata S, Osaki T, Kumaki Y, ... These small proteins contain a cysteine-stabilised triple-stranded beta-sheet with an inhibitor cystine knot motif and show ...
SPACA3
... implication of naturally occurring human antimicrobial agents beta-defensins, cathelicidin LL-37 and lysozyme". J. Dermatol. ...
Trefoil factor 3
On the other hand, TFF3 activated the epithelial cells in culture to produce beta defensins 2 (hBD2) and beta defensins 4 (hBD4 ... and promotes human beta defensin (hBD2 and hBD4) expression in intestinal epithelial cells HT-29". Bosn J Basic Med Sci. 12 (4 ... and promotes human beta defensin (hBD2 and hBD4) expression in intestinal epithelial cells HT-29". Bosn J Basic Med Sci. 12 (4 ... such as defensins. Two main mechanisms have been described for the activation of PAR-2: (A) by specific cleavage that unmask ...
Ayyalusamy Ramamoorthy
Dhople V, Krukemeyer A, Ramamoorthy A, The human beta-defensin-3, an antibacterial peptide with multiple biological functions, ...
Digital polymerase chain reaction
2010). "Measurement methods and accuracy in copy number variation: failure to replicate associations of beta-defensin copy ...
Canine terminology
The project took samples from 38 different breeds to find the gene (a beta defensin gene) responsible for dog coat color. One ... Nov 2007). "A -defensin mutation causes black coat color in domestic dogs". Science. 318 (5855): 1418-23. doi:10.1126/science. ...
West Nile- Growth Factors, Growth Hormones, Purified Proteins, Dominant Negative Clones, Constitutively Active Clones- Lifeome
PeptidesGenesANTIMICROBIAL PEPTIDEGeneAntibodyPeptideEpithelial cellsProteinHBDsHuman DefensinHost defenseSpeciesCharacterizationAvian beta-defensinHBD2MRNAInnate immuneDefb1MammalianDisulfideMolecularGenomicCysteineNeutrophilsTissuesPathwaysMajority of beta-defensinsExpression of beta-defensinsDefenseNeutrophilAntibacterial activityColiAntibodiesCathelicidinSusceptibilityInducibleNovel beta-defensinRegulationCross reactivityTheta defensinsAminoFamily of cationicVertebratesHumans
Peptides50
- The hallmark property of beta-defensins, their antimicrobial activity, is clearly only the tip of the iceberg in the extensive network of inter-relations within the immune system in which these peptides function. (nih.gov)
- The beta defensins are antimicrobial peptides implicated in the resistance of epithelial surfaces to microbial colonization. (wikipedia.org)
- Defensins are 2-6 kDa, cationic, microbicidal peptides active against many Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses, containing three pairs of intramolecular disulfide bonds. (wikipedia.org)
- These genes are responsible for production of antimicrobial peptides found in white blood cells such as macrophages, granulocytes and NK-cells, β-defensins are also found in epithelial cells. (wikipedia.org)
- Defensins are small cationic peptides that are crucial components of innate immunity, serving as both antimicrobial agents and chemoattractant molecules. (rcsb.org)
- Ganz, T. (2005) Defensins and other antimicrobial peptides: A historical perspective and an update. (scirp.org)
- Defensins are a family of cationic antimicrobial peptides that participate in innate host defence. (nih.gov)
- In particular, peptides of the β-defensin family offer antimicrobial activity against different pathogens including bacteria and fungi. (springer.com)
- This study reports the first successful recombinant expression of cationic antimicrobial peptides human beta-defensin-26 and human beta-defensin-27 in Escherichia coli . (springer.com)
- Jones DE, Bevins CL (1993) Defensin-6 messenger-Rna in human paneth cells-implications for antimicrobial peptides in host defense of the human bowel. (springer.com)
- β-defensins are among the most abundant antimicrobial peptides in mammals, and thought to be solely found in vertebrates until a recent report describing the cloning and sequencing of defensin like peptides in the spiny lobster Panulirus japonicus. (unboundmedicine.com)
- In the current study, we cloned and sequenced two genes from the hemocytes of the spiny lobster Panulirus argus encoding for two isoforms of defensin-like peptides, thus confirming the presence of this protein in the Panulirus genus. (unboundmedicine.com)
- The 44 amino acids mature peptides showed the conservation of cysteine pattern characterizing the β-defensins, as well as known amino acids residues critical to exert their antimicrobial activity. (unboundmedicine.com)
- The tertiary structure obtained by homology modeling indicated that likely conformations of lobster peptides are highly similar to β-defensins from vertebrates. (unboundmedicine.com)
- The finding of a putative defensin-like peptide in the expressed sequence tag (EST) of the lobster Homarus americanus with high homology with those of P. argus described in this study, would indicate the presence of this peptides in Palinuridae family. (unboundmedicine.com)
- Taking into account all similarities between these peptides with β-defensins from vertebrates, it is conceivable to further support the finding of a new family of β-defensins in invertebrate. (unboundmedicine.com)
- Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. (prospecbio.com)
- The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. (prospecbio.com)
- Background: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. (cellsciences.com)
- Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. (cellsciences.com)
- β-Defensins are cationic antimicrobial peptides showing little sequence similarity but highly conserved tertiary structure stabilized by a six-cysteines-motif. (ovid.com)
- The solution structure is reported for bovine neutrophil beta-defensin-12 (BNBD-12), a member of the beta-defensin family of antimicrobial peptides. (nih.gov)
- Defensins are antimicrobial peptides that play an important role in the innate immune response in the intestine. (wur.nl)
- Hybrid peptides were rationally designed through combination of functional amino acids in parent HDPs, including human cathelicidin (LL-37) and human beta-defensin (HBD)-1 to -3. (arvojournals.org)
- Antimicrobial peptides, such as beta-defensins, are essential components of the innate immune system and are the first line of defence against invaders of the eye. (dryeyezone.com)
- beta-defensins are small cationic peptides that exhibit broad spectrum antimicrobial properties. (biomedcentral.com)
- Beta-defensins are small cationic peptides that exhibit broad-spectrum antimicrobial properties and contribute to mucosal immune responses at epithelial sites. (biomedcentral.com)
- Human beta defensins (HBDs), a group of important antimicrobial peptides expressed by the epithelium, are crucial for local defense and tissue homeostasis of oral cavity. (biomedcentral.com)
- Beta-defensins are antimicrobial peptides that protect tissues and organs from infection by a variety of microorganisms. (nih.gov)
- Human-beta defensins (HBD) belong to the family of acute phase peptides and hold a broad antimicrobial spectrum that includes gram-positive and gram-negative bacteria. (hepaton.com)
- The human β-defensin (hBD) class of antimicrobial peptides is the focus of our research. (biomedcentral.com)
- Importantly, defensin peptides appear to play multiple roles in vivo and exhibit an immunomodulatory function, similar to cytokines or chemokines. (biomedcentral.com)
- The human defensins are small cationic peptides that can be divided into α- and β-defensins based on the topology of their three intramolecular cysteine disulphide bonds. (patentsencyclopedia.com)
- Human defensins are Cys-rich antimicrobial peptides which specifically react against Gram-negative bacteria. (nordicmubio.com)
- beta-Defensin genes encode broad spectrum antimicrobial cationic peptides. (ed.ac.uk)
- Paneth cells reside in the small intestine and produce antimicrobial peptides essential for the host barrier, principally human α-defensin 5 (HD5) and HD6. (pnas.org)
- α-Defensins are abundant antimicrobial peptides in polymorphonuclear leukocytes and play an important role in innate immunity. (jci.org)
- Defensins and cathelicidins constitute the two major groups of antimicrobial peptides in most mammalian species. (vin.com)
- The most abundant group of antimicrobial peptides is comprised by the α-, β- and θ-defensins. (vin.com)
- β-defensins are small (3.5-4.5 kDa) highly basic cationic peptides. (vin.com)
- Defensins are antimicrobial peptides which play a key role in innate immunity. (northwestern.edu)
- The name 'defensin' was coined in the mid 1980s, though the proteins have been variously called 'Cationic Antimicrobial Proteins', 'Neutrophil peptides', 'Gamma thionins' amongst others. (wikipedia.org)
- In addition to the defensins involved in host defence, there are a number of related Defensin-Like Peptides (DLPs) that have evolved to have other activities. (wikipedia.org)
- Polymorphonuclear neutrophils (PMNs) contain three α-defensin peptides, called HNP-1, -2, and -3 ( 18 , 59 ). (asm.org)
- Although the studies described below focused on human α-defensins, some studies also examined human β-defensin peptides hBD-1, hBD-2, and hBD-3. (asm.org)
- The antimicrobial peptides human β-defensin-1 (hBD1), hBD2, hBD3, and CAP18 expressed by keratinocytes have been implicated in mediation of the innate defense against bacterial infection. (asm.org)
- Human beta defensins (HBDs), small cationic antimicrobial peptides that exert a broad-spectrum antibacterial function at epithelial surfaces and within some mesenchymal tissues, could probably help to reduce such contamination. (edu.au)
- As components of the innate immune system, antimicrobial peptides in the form of human defensins play an important role in host defense by serving as the epithelial layer's biochemical barrier against local infections. (mdpi.com)
- Defensins are small (3.5~5 kDa) cationic antimicrobial peptides that have a broad spectrum of activity that includes gram-negative bacterias, yeasts and enveloped viruses . (bvsalud.org)
- β-defensins, initially identified as broad-spectrum antimicrobial peptides, increasingly have been observed to exhibit numerous other in vitro and in vivo activities that do not always relate directly to host defense. (biomedcentral.com)
Genes21
- β-defensins are coding for genes which impact the function of the innate immune system. (wikipedia.org)
- Single-nucleotide polymorphisms (SNPs) are found in genes coding for β-defensins. (wikipedia.org)
- β-defensins genes are found across the vertebrates, including mammals, reptiles, birds and fish. (wikipedia.org)
- Expression of beta-defensin genes by human salivary glands. (nih.gov)
- This study investigated expression of genes encoding human beta-defensins 1 and 2 by human salivary glands. (nih.gov)
- Discover related pathways, diseases and genes to Defensin beta 2. (novusbio.com)
- The phylogenetic analysis using β-defensin coding sequences of different snakes grouped them in two main branches: genes organized in three or two exons. (ovid.com)
- Previous work has suggested copy number variation of a cluster of beta-defensin genes affects HIV load in treatment-na " ive sub-Saharan Africans and rate of response to antiretroviral treatment. (epfl.ch)
- Previous work has shown that it is encoded in a cluster of beta-defensin genes at 8p23.1, which varies in copy number between 2 and 12 in different individuals. (cfgenetherapy.org.uk)
- Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. (mybiosource.com)
- This gene is found in a cluster with other beta-defensin genes on the long arm of chromosome 20. (nih.gov)
- Two gene knock down methods - dominant negative (DN) plasmid and small interfering RNA (siRNA) - were employed to detect and confirm the involvement of several key genes in the signaling cascade resulting from the NTHi stimulated β-defensin 2 expression in human middle ear epithelial cell (HMEEC-1). (biomedcentral.com)
- Over 30 previously unrecognized β-defensin genes have recently been identified in the human genome, many with yet to be determined functions. (biomedcentral.com)
- All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. (genecards.org)
- Using hidden Markov models, we identified novel mouse and human beta-defensin genes. (ed.ac.uk)
- In order to look at function of these rapidly evolving genes, we characterised one of the novel mouse beta-defensin genes. (ed.ac.uk)
- The activation of the TLRs and the NF- κ B pathway is responsible for the induction of the expression of antimicrobial peptide genes, including beta-defensins. (vin.com)
- In all families, the underlying genes responsible for defensin production are highly polymorphic . (wikipedia.org)
- In general, both α- and β-defensins are encoded by two- exon genes, where the first exon encodes for a hydrophobic leader sequence (that is removed after translation ) and the cysteine-rich sequence (the mature peptide). (wikipedia.org)
- GWAS for baseline plasma KYN concentrations identified SNPs across the beta-defensin 1 (DEFB1) and aryl hydrocarbon receptor (AHR) genes that were cis-expression quantitative trait loci (eQTLs) for DEFB1 and AHR mRNA expression, respectively. (cdc.gov)
- The adaptation of chiropterans to different environments and ecological niches with diverse pathogen profiles seems to have shaped the evolution of Defensin beta 103a and hepcidin genes in a suborder and family specific manner respectively. (ntu.edu.sg)
ANTIMICROBIAL PEPTIDE7
- Bajaj-Elliott M, Fedeli P, Smith GV, Domizio P, Maher L, Ali RS, Quinn AG, Farthing MJ (2002) Modulation of host antimicrobial peptide (beta-defensins 1 and 2) expression during gastritis. (springer.com)
- Beta Defensin-1 is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. (prospecbio.com)
- Recombinant human beta-defensin-2 (BD-2) is an antimicrobial peptide which belongs to the distinct family of beta-defensins. (genetex.com)
- Recombinant human Defensin beta 3 contains 45 amino acid residues and is an antimicrobial peptide belonging to the distinct family of beta-defensins. (cellsciences.com)
- 2. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. (nordicmubio.com)
- 1999) Mouse ß-Defensin 3 is an inducible antimicrobial peptide expressed in the epithelia of multiple organs. (exalpha.com)
- To examine the effect of the natural antimicrobial peptide human β-defensin-3 (hBD-3), on the migration of a head and neck cancer cell line in vitro using microfabrication and soft-lithographic techniques. (biomedcentral.com)
Gene20
- Growing interest in beta-defensins steadily enhances our knowledge about various aspects of their gene location, expression patterns and the transcription factors involved in their regulation in vivo. (nih.gov)
- The fact that alpha and theta defensins are absence in older vertebrates, like birds and fishes, indicates that defensins must have evolved from the same ancestral gene coding for β-defensins. (wikipedia.org)
- Beta-defensin 3 is a protein that in humans is encoded by the DEFB3 gene. (wikipedia.org)
- Thus, human beta-defensin 1 gene expression occurred in all human major and minor salivary glands studied, whereas human beta-defensin 2 expression occurred only in a small number of gland samples. (nih.gov)
- This gene encodes defensin, beta 103A, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. (prospecbio.com)
- We set-up a quantitative PCR method to detect the gene expression of pBD-1 and a newly discovered porcine ß-defensin, pBD-2. (wur.nl)
- This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. (mybiosource.com)
- The protein encoded by this gene is secreted and is a member of the beta defensin protein family. (mybiosource.com)
- RNAs isolated from male reproductive tract tissues of rat were reverse transcribed and PCR amplified using gene specific primers for defensins. (biomedcentral.com)
- OBJECTIVE To investigate the possible association between the SNP in the 5' untranslated region (5' UTR) of the human beta defensin 1 (DEFB1) gene and the susceptibility to pulmonary tuberculosis (PTB) in Chinese Han population. (semanticscholar.org)
- This gene encodes a member of the beta subfamily of defensins. (nih.gov)
- By 2-color fluorescence in situ hybridization on both metaphase chromosomes and released chromatin fiber, the DEFB1gene is mapped to 8p23.2-p23.1, within 100 to 150 kb of the gene for human neutrophil defensin-alpha-1. (bosterbio.com)
- DEFB107A (Defensin Beta 107A) is a Protein Coding gene. (genecards.org)
- This gene deviates from the canonical six cysteine motif present in the mature functional peptide of all other beta defensins. (ed.ac.uk)
- This defensin related gene (Defr1) is most highly expressed in testis and heart and the genomic organisation is highly similar to Defb3-6. (ed.ac.uk)
- High gene expression levels of α-defensin-3 and -4 have been detected in benign oral neoplasia, α-defensin-6 is considered to be a tumor marker for colon cancer. (mdpi.com)
- Reverse transcription polymerase chain reaction (RT-PCR) was used to semiquantitate HBD-1 and HBD-2 mRNA using the housekeeping gene beta-actin as an internal control . (bvsalud.org)
- There have been conflicting reports in the literature on association of gene copy number with disease, including CCL3L1 and HIV susceptibility, and ß-defensins and Crohn's disease. (forskningsdatabasen.dk)
- In conclusion, these observed evolutionary patterns and unique tissue gene expression profiles (defensin beta 103 and hepcidin) foretell potential functional difference between human and bat AMPs. (ntu.edu.sg)
- The objective of the study was to clone avian beta-defensin (AvBD) 5 gene from pigeon bone marrow tissues and liver tissues , to express the recombinant AvBD5 protein in E. coli, and to determine its antimicrobial activity. (bvsalud.org)
Antibody11
- Each beta-Defensin 4/2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
- Choose from our beta-Defensin 4/2 polyclonal antibodies and browse our beta-Defensin 4/2 monoclonal antibody catalog. (novusbio.com)
- The antibody was purified by affinity chromatography employing immobilized human beta 2 Defensin matrix. (genetex.com)
- This antibody is specific for beta 2 Defensin. (genetex.com)
- This antibody reacts with Human BD3 (Defensin beta 3). (acris-antibodies.com)
- Rabbit IgG polyclonal antibody for Beta-defensin 1(DEFB1) detection. (bosterbio.com)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle Defensin Beta 103A (DEFb103A) in serum, plasma and other biological fluids. (gkts.net)
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103A (DEF?103A). (gkts.net)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Defensin Beta 119 (DEFb119) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (gkts.net)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Defensin Beta 119 (DEFb119) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (gkts.net)
- View detailed β-defensin 2 antibody specifications by linking to the specific product blocks. (scbt.com)
Peptide9
- Harder J, Bartels J, Christophers E, Schroder JM (2001) Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic. (springer.com)
- Garcia JRC, Krause A, Schulz S, Rodriguez-Jimenez FJ, Kluver E, Adermann K, Forssmann U, Frimpong-Boateng A, Bals R, Forssmann WG (2001) Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity. (springer.com)
- An ensemble of structures with low NOE constraint violation energies revealed a precisely defined triple-stranded, antiparallel beta-sheet as the structural core of the peptide. (nih.gov)
- The authors characterize the recombinant human beta-defensin DEFB114 peptide, demonstrating its anti-microbial potential, lipopolysaccharide (LPS) binding and neutralization, and anti-inflammatory effects in vitro and in vivo. (moleculardevices.com)
- A synthetic peptide corresponding to a sequence at the C-terminus of human beta Defensin 1(52-68aa FTKIQGTCYRGKAKCCK). (bosterbio.com)
- Human beta-defensins (hBDs) belong to a family of small cationic peptide antibiotics produced by human epithelial cells and are characterized by wide spectrum of biologic activities. (exp-oncology.com.ua)
- The peptide sequences of HBD-3/MBD-3 used for immunisation contain very little consensus aa with any of the other human or mouse defensins. (exalpha.com)
- Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. (genecards.org)
- alpha, beta, theta (circle w/horizontal line)] Mammalian defensins are endogenous cysteine-rich peptide antibiotics classically produced either by epithelial cells of the respiratory, urogenital and digestive tracts, or by circulating cells including granulocytes and macrophages. (vin.com)
Epithelial cells7
- However, in other species, beta-defensins are more often produced by epithelial cells lining various organs (e.g. the epidermis, bronchial tree and genitourinary tract. (wikipedia.org)
- Beta Defensin is highly expressed by epithelial cells. (prospecbio.com)
- Our hypothesis is that cigarette smoke exposure will affect the expression of β-defensins in human lung alveolar epithelial cells (A549). (biomedcentral.com)
- α-defensins are not expressed in epithelial cells, and were not measured in the present study. (biomedcentral.com)
- The β-defensins are mainly produced by epithelial cells in various tissues and organs including the skin, trachea, gastrointestinal tract, urogenital system, kidneys, pancreas and mammary gland. (patentsencyclopedia.com)
- Beta-defensins are involved also in wound healing processes and may affect proliferation of epithelial cells. (exp-oncology.com.ua)
- In recent years it has been shown that epithelial cells are capable of actively counteracting H. pylori by the secretion of human beta defensin 3 (hBD-3). (fu-berlin.de)
Protein4
- Although the shape and charge distribution of the monomer are similar to those of other defensins, an additional alpha-helical region makes this protein topologically distinct from the mammalian alpha- and beta-defensin structures reported previously. (rcsb.org)
- Members of the defensin family are highly similar in protein sequence. (prospecbio.com)
- Human beta-defensin 2 (DEFB4, also known as DEFB2 or hBD-2) is a salt-sensitive antimicrobial protein that is expressed in lung epithelia. (cfgenetherapy.org.uk)
- [23] Other invertebrates known to produce defensins from this protein superfamily include molluscs , annelids and cnidaria . (wikipedia.org)
HBDs2
- PURPOSE: To investigate human beta-defensins (HBDs) and cathelicidin LL-37 (LL-37) expressions in patients with pterygium. (jefferson.edu)
- 3 Among the most promising AMPs is the human β-defensins (hBDs) family that displays potent antimicrobial activity against gram-positive and gram-negative bacteria, fungi, viruses, and parasites. (dovepress.com)
Human Defensin2
- Should the Human Defensin Beta 103A (DEFb103A) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement. (gkts.net)
- Description: A sandwich quantitative ELISA assay kit for detection of Human Defensin Beta 103A (DEFb103A) in samples from serum, plasma or other biological fluids. (gkts.net)
Host defense4
- Schutte, B.C. and McCray, P.B.J. (2002) Beta-defensins in lung host defense. (scirp.org)
- Ganz T (1999) Immunology-defensins and host defense. (springer.com)
- Upregulated HBD-2 expression in renal tubulus epithelium indicates a role of a wider range of human defensins for antimicrobial host defense in the urogenital tract than previously recognized. (biomedcentral.com)
- Here, we show that healthy monocytes can induce the expression of these defensins via Wnt ligands, especially in patients with Crohn's disease (CD), who normally display reduced levels of human α-defensin 5 (HD5) and HD6, resulting in impaired host defense and, consequently, mucosal inflammation. (pnas.org)
Species8
- Every mammalian species explored thus far has beta-defensins. (wikipedia.org)
- pression in whole-heart-homogenate has been reported in different species, and plasma alpha-defensins have been associated with cardiovascular morbidity and mortality. (scirp.org)
- Our beta-Defensin 4/2 Antibodies can be used in a variety of model species: Human. (novusbio.com)
- Using a PCR approach, we described β-defensin sequences with two exons in three species of Colubridae snakes with high sequence similarity between them. (ovid.com)
- More recently, however, β-defensins have also been identified in the heart of different species. (vin.com)
- Both beta-defensins and their signaling molecules, Toll-Like Receptors, have been identified in the heart of various species. (vin.com)
- Select appropriate β-defensin 2 antibodies for your research by isotype, epitope, applications and species reactivity. (scbt.com)
- In addition, phylogenetic relationships between amino acid sequence of the pigeon AvBD5, AvBDs from other avian species, and some mammalian beta-defensin -5 were analyzed. (bvsalud.org)
Characterization3
- The last decade led to the discovery and characterization of several human beta-defensins. (nih.gov)
- Characterization of novel defensins in the male reproductive tract can lead to increased understanding of their dual roles in immunity and sperm maturation. (biomedcentral.com)
- In this study, characterization of Defensin beta 103a, hepcidin and cathelicidin is reported for the first time in bats. (ntu.edu.sg)
Avian beta-defensin1
- Your search returned 2 avian beta-defensin 2 ELISA ELISA Kit across 1 supplier. (biocompare.com)
HBD25
- Human, rabbit and guinea-pig beta-defensins, as well as human beta-defensin-2 (hBD2), induce the activation and degranulation of mast cells, resulting in the release of histamine and prostaglandin D2. (wikipedia.org)
- Here, we report two high resolution x-ray structures of human beta-defensin-2 (hBD2). (rcsb.org)
- These structures provide the first detailed description of dimerization of beta-defensins, and we postulate that the mode of dimerization of hBD2 is representative of other beta-defensins. (rcsb.org)
- The structural and electrostatic properties of the hBD2 octamer support an electrostatic charge-based mechanism of membrane permeabilization by beta-defensins, rather than a mechanism based on formation of bilayer-spanning pores. (rcsb.org)
- The discovery of human β-defensin 2 (hBD2), as a Kv1.3 channel inhibitor with the unique molecular mechanism and novel immune modulatory function, suggests that human β-defensins are a novel class of channel ligands. (usda.gov)
MRNA6
- Page, R.A. and Malik, A.N. (2003) Elevated levels of beta defensin-1 mRNA in diabetic kidneys of GK rats. (scirp.org)
- Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human Beta Defensin-1 mRNA expression and higher human Beta Defensin-2 and human Beta Defensin-3 mRNA expression. (prospecbio.com)
- In a prior exploratory study investigating patients with exacerbation of chronic obstructive pulmonary disease (COPD), the investigators demonstrated a highly significant correlation between the expression level of Defensin-alpha 4 (DEFA4) mRNA in blood and the adrenal function assessed via low-dose ACTH tests. (clinicaltrials.gov)
- β-defensin-1 mRNA expression has been identified in the heart of mice (murine beta-defensin-1, mBD1), pig (porcine beta-defensin-1, pBD1), and horse (equine beta-defensin-1, eBD1). (vin.com)
- To understand beta-defensin function in H. pylori-associated chronic gastritis, we investigated susceptibility, human beta-defensin mRNA expression, and DNA methylation changes to promoters in the gastric mucosa with or without H. pylori infection. (gastrocourse.ru)
- High levels of human beta defensin-1 (hBD-1) have previously been detected in the kidney and pancreas, but the cell-specific location of hBD-1 mRNA has not been determined. (northwestern.edu)
Innate immune2
- Defensins not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site. (wikipedia.org)
- Among its related pathways are Defensins and Innate Immune System . (genecards.org)
Defb11
- DEFB1 belongs to the defensin family. (bosterbio.com)
Mammalian2
- Beta defensins are a family of mammalian defensins. (wikipedia.org)
- On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. (wikipedia.org)
Disulfide6
- The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. (cellsciences.com)
- Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. (cellsciences.com)
- Expression of at least two subfamilies of defensins (α- and β-defensins) has been shown in insects, plants and humans in respect to a difference in the position and connectivity of cystein disulfide bonds [ 1 , 2 ]. (biomedcentral.com)
- They are classified as α-, β-, and θ-defensins depending on their disulfide bond pairing and secondary structure. (biomedcentral.com)
- We have designed a cyclic 17-amino acid β-defensin analog featuring a single disulfide bond. (dovepress.com)
- The defensins contain six cysteine residues forming three disulfide bridges depending on the spacing of the cysteine residues and the connectivity of the disulfide bridge, defensins are classified into two families , the alpha-defensins (HNP) and beta-defensins (HBD). (bvsalud.org)
Molecular5
- Structural studies of beta-defensins provide the molecular basis for a better understanding of their properties, functions and their potential for practical applications. (nih.gov)
- Beta Defensin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5161.2 Dalton. (antibody-antibodies.com)
- Establishing differential β-defensin expression following CSE treatment will add to our understanding of the molecular response of the lung alveolar epithelium to cigarette smoke exposure. (biomedcentral.com)
- Beta Defensin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. (antibody-antibodies.com)
- Here we examined the molecular mechanism(s) of α-defensin-1-mediated HIV-1 inhibition. (jci.org)
Genomic4
- Analysis of genomic information indicates that the number of beta-defensin-like molecules encoded by the human genome may number in the tens. (nih.gov)
- Beta-defensin genomic copy number is not a modifier locus for cystic fibrosis. (cfgenetherapy.org.uk)
- We determined the copy number of this locus in 355 patients with cystic fibrosis (CF), and tested for correlation between beta-defensin cluster genomic copy number and lung disease associated with CF. No significant association was found. (cfgenetherapy.org.uk)
- In silico rat genomic analyses were used to identify novel beta-defensins related to human defensins 118-123. (biomedcentral.com)
Cysteine2
- Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. (mybiosource.com)
- β-defensins are structurally defined by the conserved cysteine-rich motif forming three disulphide bonds, which stabilize a β-sheet formation. (vin.com)
Neutrophils1
- In cows, as many as 13 beta-defensins exist in neutrophils. (wikipedia.org)
Tissues5
- Plants defensins were discovered in 1990 and have subsequently been found in most plant tissues with antimicrobial activities against both bacteria. (wikipedia.org)
- Defensins play a role in cell division, attraction and maturation of immune cells, differentiation and reorganization of epithelial tissues, wound healing and tumor suppression. (mdpi.com)
- qPCR was carried out on a panel of bat tissues and the panel of expression of β defensin 103a and hepcidin is very different between the bat and the human. (ntu.edu.sg)
- Notably, an increased expression of defensin beta 103a and hepcidin is reported in many bat tissues as compared to humans. (ntu.edu.sg)
- Human β-defensin (hBD) expression has been identified in many tissues, almost uniformly in the epithelium of the oral cavity. (biomedcentral.com)
Pathways1
- We selected most pathways defb104a participated on our site, such as Beta defensins, Defensins, Immune System, which may be useful for your reference. (creativebiomart.net)
Majority of beta-defensins1
- The majority of beta-defensins identified in humans are predominantly expressed in the male reproductive tract and have roles in non-immunological processes such as sperm maturation and capacitation. (biomedcentral.com)
Expression of beta-defensins2
- High concentrations of L-arginine and L-lysine are necessary for the expression of beta-defensins. (dryeyezone.com)
- The expression of beta-defensins was detected by quantitative RT-PCR. (biomedcentral.com)
Defense3
- Constitutive expression and localization of antimicrobial human β-defensin-1 (HBD-1) in human kidneys as a potential mechanism of antimicrobial defense has been previously reported. (biomedcentral.com)
- Human beta defensins (HBD)-1, HBD-2, and HBD-3 are key elements of innate immunity that represent the first line of defense against different pathogen microorganisms associated with preterm labor. (biomedcentral.com)
- Epithelial β-defensins constitute a rapidly mobilized local defense against microbial intruders at the epithelial and mucosal surfaces, and different studies have reported induction of epithelial β-defensins at sites of inflammation, injury, infection as well as other disease processes. (vin.com)
Neutrophil2
- The α-defensins can be further subdivided into those that were first isolated from neutrophil granules (HNP1-4) and those that are expressed by Paneth cells in the crypts of the small intestine (HD5 and HD6). (patentsencyclopedia.com)
- An organism usually produces many different defensins, some of which are stored inside the cells (e.g. in neutrophil granulocytes to kill phagocytosed bacteria), and others are secreted into the extracellular medium. (wikipedia.org)
Antibacterial activity1
- defensin, beta 2: Has antibacterial activity (Potential). (mybiosource.com)
Coli6
- Huang L, Ching CB, Jiang RR, Leong SSJ (2008) Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression. (springer.com)
- Huang L, Wang JF, Zhong ZX, Peng L, Chen HQ, Xu ZN, Cen PL (2006) Production of bioactive human beta-defensin-3 in Escherichia coli by soluble fusion expression. (springer.com)
- E.coli derived recombinant Human Beta Defensin-3 (Cat. (acris-antibodies.com)
- Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. (acris-antibodies.com)
- Recombinant defensins were tested against E. coli in a colony forming unit assay to analyze their antimicrobial activities. (biomedcentral.com)
- Both recombinant plasmids were transformed into E. coli BL21 and the bacteria were induced with Isopropyl beta-D-1-Thiogalactopyranoside ( IPTG ). (bvsalud.org)
Antibodies6
- We offer beta-Defensin 4/2 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Sandwich ELISA, Western Blot. (novusbio.com)
- 17 Defensin beta 3 (DEFB3) ELISA Kits from 5 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
- GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Recombinant Human beta Defensin-3. (antibody-antibodies.com)
- 15 Defensin, alpha 1 (DEFA1) ELISA Kits from 2 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
- Santa Cruz Biotechnology, Inc. offers a broad range of β-defensin 2 antibodies. (scbt.com)
- Select β-defensin 2 antibodies from monoclonal antibodies listed below. (scbt.com)
Cathelicidin3
- An investigation of human beta-defensins and cathelicidin expression i" by Remzi Karadag, Nurettin Bayram et al. (jefferson.edu)
- An investigation of human beta-defensins and cathelicidin expression in patients with pterygium. (jefferson.edu)
- In addition to α and β defensins, humans possess one member of the cathelicidin class of AMP, LL-37, which was also assessed in these experiments. (biomedcentral.com)
Susceptibility1
- Association between human beta defensin-1 single nucleotide polymorphisms and susceptibility to pulmonary tuberculosis]. (semanticscholar.org)
Inducible2
- Inducible expression of human β-defensin-2 (HBD-2) has been described in various epithelial organs but not for the urogenital tract. (biomedcentral.com)
- Among the AIIMs, human β-defensin 2 is the most potent molecule and is inducible by exposure to inflammatory stimuli such as bacterial components or proinflammatory cytokines. (biomedcentral.com)
Novel beta-defensin1
- Bensch KW, Raida M, Magert HJ, Schulz-Knappe P, Forssmann WG (1995) hBD-1: a novel beta-defensin from human plasma. (springer.com)
Regulation9
- RT-competitive multiplex PCR is a simple and accurate method that enables new insights into defensin regulation under physiological and pathophysiological conditions. (springer.com)
- In this review, we summarize current knowledge on the human CATs that appear to be integrated in causal regulation cascades of beta-defensins, thereby offering novel concepts for therapeutic perspectives. (dryeyezone.com)
- RT-PCR analysis was performed to analyze the tissue distribution, developmental expression and androgen regulation of these defensins. (biomedcentral.com)
- However, very little is known about the androgenic regulation on the production and secretion of the epididymal beta-defensins. (biomedcentral.com)
- The present study provides novel insights into the mechanisms of androgen regulation on epididymal beta-defensins, enabling a better understanding of the function of beta-defensins in sperm maturation and fertility. (biomedcentral.com)
- However, up to now, very little is known about the regulation of the production and secretion of epididymal beta-defensins. (biomedcentral.com)
- This study found that the induction of β-defensin 2 is highest in whole cell lysate (WCL) preparations of NTHi, suggesting that the ligand(s) responsible for this up-regulation may be soluble macromolecule(s). (biomedcentral.com)
- Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. (nordicmubio.com)
- Paneth cell defensins (human α-defensins 5 and 6) are central players in the regulation of the human microbiome in the small intestine, but their regulation remains unclear. (pnas.org)
Cross reactivity3
- ß-defensin-1 (HBD-1) whole molecule and 17AA N-terminal fraction of HBD-1, no cross-reactivity to alpha-defensin and other beta-defensins. (nordicmubio.com)
- ß-defensin-3 of human and mouse origin, N-terminal fragments of HBD-3 and MBD-3, no cross-reactivity with other known ß-defensins or alpha-defensins. (exalpha.com)
- ß-defensin-2 (HBD-2) whole molecule and N-terminal fragments of HBD-2, no cross-reactivity to alpha-defensin and other beta-defensins, full cross-reaction with mouse and rat beta-defensin-2. (exalpha.com)
Theta defensins1
- [17] [18] Theta defensins form a single beta hairpin structure and therefore also represent a distinct group. (wikipedia.org)
Amino5
- Pore complex will be created as a result of breaking the hydrogen bonds between the amino acids in the terminal end of the strands connecting defensins monomers. (wikipedia.org)
- The deduced amino acid sequence presented the characteristics of β-defensin family. (ovid.com)
- 10. The method of claim 1, wherein any amino acid substitution in the beta-defensin relative to SEQ ID NO: 5 is a conservative substitution. (patentsencyclopedia.com)
- Amino acid sequences of alpha-defensins 1-4 (HNP1-4) and beta-Defensins 1-4 (HBD1-4) are known. (nordicmubio.com)
- Beta-defensin-1 and beta-defensin-2 share 34% amino acid sequence homology. (nordicmubio.com)
Family of cationic1
- Defensins are a family of cationic proteins (3-4 kD) typically containing 6 cystein residues. (biomedcentral.com)
Vertebrates2
- The phylogenetic study carried out by probabilistic methods confirmed the relation with ancestral β-defensin from vertebrates. (unboundmedicine.com)
- Although the most well studied defensins are from vertebrates, a family of trans-defensins called ' big defensins ' are found in molluscs , arthropods and lancelets . (wikipedia.org)
Humans5
- At least four β-defensins (HBD-1, HBD-2, HBD-3, HBD-4) have been characterized in humans. (biomedcentral.com)
- The majority of beta-defensin family members are exclusively expressed in the epididymis, and some members have been shown to play essential roles in sperm maturation and fertility in rats, mice and humans. (biomedcentral.com)
- In humans the predominant AMPs are the defensins. (patentsencyclopedia.com)
- Only alpha and beta defensins are expressed in humans. (wikipedia.org)
- The other human α-defensins, HD-5 and HD-6, are primarily expressed in small intestinal Paneth cells and are believed to protect the intestinal tract against food- and waterborne pathogens, to modulate the intestinal flora, and to be key factors in the pathogenesis of inflammatory bowel disease in genetically susceptible humans ( 3 , 67 ). (asm.org)