Progesterone Congeners: Steroidal compounds related to PROGESTERONE, the major mammalian progestational hormone. Progesterone congeners include important progesterone precursors in the biosynthetic pathways, metabolites, derivatives, and synthetic steroids with progestational activities.Crystallins: A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. The presence of these proteins accounts for the transparency of the lens. The family is composed of four major groups, alpha, beta, gamma, and delta, and several minor groups, which are classed on the basis of size, charge, immunological properties, and vertebrate source. Alpha, beta, and delta crystallins occur in avian and reptilian lenses, while alpha, beta, and gamma crystallins occur in all other lenses.beta-Crystallin B Chain: The basic subunit of beta-crystallins.beta-Crystallin A Chain: The acidic subunit of beta-crystallins.Lens, Crystalline: A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION.gamma-Crystallins: A subclass of crystallins that found in the lens (LENS, CRYSTALLINE) of VERTEBRATES. Gamma-crystallins are similar in structure to BETA-CRYSTALLINS in that they both form into a Greek key-like structure. They are composed of monomeric subunits.beta-Crystallins: A class of crystallins that provides refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Beta-crystallins are similar in structure to GAMMA-CRYSTALLINS in that they both contain Greek key motifs. Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits.Ricin: A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.alpha-Crystallin A Chain: One of the subunits of alpha-crystallins. Unlike ALPHA-CRYSTALLIN B CHAIN the expression of ALPHA-CRYSTALLIN A CHAIN is limited primarily to the lens (LENS, CRYSTALLINE).alpha-Crystallins: A subclass of crystallins that provides the majority of refractive power and translucency to the lens (LENS, CRYSTALLINE) in VERTEBRATES. Alpha-crystallins also act as molecular chaperones that bind to denatured proteins, keep them in solution and thereby maintain the translucency of the lens. The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits.alpha-Crystallin B Chain: One of the alpha crystallin subunits. In addition to being expressed in the lens (LENS, CRYSTALLINE), alpha-crystallin B chain has been found in a variety of tissues such as HEART; BRAIN; MUSCLE; and KIDNEY. Accumulation of the protein in the brain is associated with NEURODEGENERATIVE DISEASES such as CREUTZFELDT-JAKOB SYNDROME and ALEXANDER DISEASE.delta-Crystallins: A subclass of crystallins found in the lens (LENS, CRYSTALLINE) in BIRDS and REPTILES. They are inactive forms of the enzyme argininosuccinate lyase.Cataract: Partial or complete opacity on or in the lens or capsule of one or both eyes, impairing vision or causing blindness. The many kinds of cataract are classified by their morphology (size, shape, location) or etiology (cause and time of occurrence). (Dorland, 27th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Abrin: A toxic lectin from the seeds of jequirity, Abrus precatorius L. Very active poison. Five different proteins have so far been isolated: Abrus agglutinin, the component responsible for: hemagglutinating activity, & abrins a-d, the toxic principals each consisting of two peptide chains are held together by disulfide bonds.Bungarus: A genus of poisonous snakes of the subfamily Elapinae of the family ELAPIDAE. They comprise the kraits. Twelve species are recognized and all inhabit southeast Asia. They are considered extremely dangerous. (Moore: Poisonous Snakes of the World, 1980, p120)Interleukin-1beta: An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.beta 2-Microglobulin: An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.Cataract Extraction: The removal of a cataractous CRYSTALLINE LENS from the eye.Genes, Dominant: Genes that influence the PHENOTYPE both in the homozygous and the heterozygous state.Pedigree: The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.Genetic Linkage: The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Thrombin: An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Students: Individuals enrolled in a school or formal educational program.Faculty, Medical: The teaching staff and members of the administrative staff having academic rank in a medical school.Faculty: The teaching staff and members of the administrative staff having academic rank in an educational institution.Students, Medical: Individuals enrolled in a school of medicine or a formal educational program in medicine.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Mechlorethamine: A biologic alkylating agent that exerts its cytotoxic effects by forming DNA ADDUCTS and DNA interstrand crosslinks, thereby inhibiting rapidly proliferating cells. The hydrochloride is an antineoplastic agent used to treat HODGKIN DISEASE and LYMPHOMA.Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Chromomycin A3: Glycosidic antibiotic from Streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.Odontogenic Cysts: Cysts found in the jaws and arising from epithelium involved in tooth formation. They include follicular cysts (e.g., primordial cyst, dentigerous cyst, multilocular cyst), lateral periodontal cysts, and radicular cysts. They may become keratinized (odontogenic keratocysts). Follicular cysts may give rise to ameloblastomas and, in rare cases, undergo malignant transformation.Reticular Formation: A region extending from the PONS & MEDULLA OBLONGATA through the MESENCEPHALON, characterized by a diversity of neurons of various sizes and shapes, arranged in different aggregations and enmeshed in a complicated fiber network.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Oxidoreductases, N-DemethylatingInformation Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Semiconductors: Materials that have a limited and usually variable electrical conductivity. They are particularly useful for the production of solid-state electronic devices.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Fertile Period: The period in the MENSTRUAL CYCLE that is optimal for FERTILIZATION of the OVUM and yielding PREGNANCY. The duration of fertile period depends on the life span of male GAMETES within the female reproductive tract and the time of OVULATION. It usually begins a few days before ovulation and ends on the day of ovulation.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Desmin: An intermediate filament protein found predominantly in smooth, skeletal, and cardiac muscle cells. Localized at the Z line. MW 50,000 to 55,000 is species dependent.

Genetic heterogeneity of the Coppock-like cataract: a mutation in CRYBB2 on chromosome 22q11.2. (1/70)

PURPOSE: To identify the genetic defect for the Coppock-like cataract (CCL) affecting a Swiss family, which defect was unlinked to the chromosome 2q33-35 CCL locus. METHODS: A large family was characterized for linkage analysis by slit lamp examination or by the review of drawings made before cataract extraction. The affection status was attributed before genotyping, and the genotyping was masked to the affection status. Two-point and multipoint linkage analyses were performed using the MLINK and the LINKMAP components of the LINKAGE program package (ver. 5.1), respectively. Mutational analysis of candidate genes was performed by a combination of direct cycle sequencing and an amplification refractory mutation system assay. RESULTS: Ten individuals were affected with the CCL phenotype. The disease was autosomal dominant and appeared to be fully penetrant. A new CCL locus was identified on chromosome 22q11.2 within a 11.67-cM interval (maximum lod score [Zmax] = 4.14; theta = 0). Mutational analysis of the CRYBB2 candidate gene identified a disease-causing mutation in exon 6. This sequence change was identical with that previously described to be associated with the cerulean cataract, a clinically distinct entity. CONCLUSIONS: The CCL phenotype is genetically heterogeneous with a second gene on chromosome 22q11.2, CRYBB2. The CCL and the cerulean cataract are two distinct clinical entities associated with the same genetic defect. This work provides evidence for a modifier factor that influences cataract formation and that remains to be identified.  (+info)

Expression of betaB(2)-crystallin mRNA and protein in retina, brain, and testis. (2/70)

PURPOSE: To evaluate the expression of betaB(2)-crystallin mRNA and protein in rat, bovine, and human nonlens and nonocular tissues. METHODS: betaB(2)-crystallin mRNA levels were detected by RT-PCR. betaB(2)-crystallin protein was purified from rat and bovine tissues by FPLC chromatography. FPLC fractions were analyzed by immunoblotting. The identity of betaB(2)-crystallin protein, isolated from the retina, was confirmed by protein microsequencing. RESULTS: betaB(2)-crystallin transcript was detected in rat brain, rat testis, and human retina by RT-PCR. betaB(2)-crystallin transcript was not found in rat lung, heart, ovary, spleen, thymus, kidney, and liver or in human brain and testis. betaB(2)-crystallin protein was partially purified from and its identity confirmed in rat brain, rat testis, and bovine retina. The bovine retinal protein was further confirmed to be authentic betaB(2)-crystallin by protein microsequencing. CONCLUSIONS: These results establish that betaB(2)-crystallin mRNA and protein are expressed in tissues outside of the lens and outside of the eye including retina, brain, and testis. Extralenticular and extraocular expression of betaB(2)-crystallin, coupled with its participation in phosphorylation pathways, suggests that it has nonrefractive functions in these tissues.  (+info)

Aey2, a new mutation in the betaB2-crystallin-encoding gene of the mouse. (3/70)

PURPOSE: During an ethylnitrosourea (ENU) mutagenesis screen, mice were tested for the occurrence of dominant cataracts. One particular mutant was found that caused progressive opacity and was referred to as Aey2. The purpose of the study was to provide a morphologic description, to map the mutant gene, and to characterize the underlying molecular lesion. METHODS: Isolated lenses were photographed, and histologic sections of the eye were analyzed according to standard procedures. Linkage analysis was performed using a set of microsatellite markers covering all autosomal chromosomes. cDNA from candidate genes was amplified after reverse transcription of lens mRNA. RESULTS: The cortical opacification visible at eye opening progressed to an anterior suture cataract and reached its final phenotype as total opacity at 8 weeks of age. There was no obvious difference between heterozygous and homozygous mutants. The mutation was mapped to chromosome 5 proximal to the marker D5Mit138 (8.7 +/- 4.2 centimorgan [cM]) and distal to D5Mit15 (12.8 +/- 5.4 cM). No recombinations were observed to the markers D5Mit10 and D5Mit25. This position makes the genes within the betaA4/betaB-crystallin gene cluster excellent candidate genes. Sequence analysis revealed a mutation of T-->A at position 553 in the Crybb2 gene, leading to an exchange of Val for GLU: It affects the same region of the Crybb2 gene as in the Philly mouse. Correspondingly, the loss of the fourth Greek key motif is to be expected. CONCLUSIONS: The Aey2 mutant represents the second allele of Crybb2 in mice. Because an increasing number of beta- and gamma-crystallin mutations have been reported, a detailed phenotype-genotype correlation will allow a clearer functional understanding of beta- and gamma-crystallins.  (+info)

Identification and properties of anti-chaperone-like peptides derived from oxidized bovine lens betaL-crystallins. (4/70)

Thermal aggregation of betaL-crystallin was higher in the presence of peptide fragments generated from oxidized and trypsin-digested betaL-crystallin compared with thermal aggregation of the control proteins without oxidized betaL-crystallin fragments. Increased aggregation of betaL-crystallin was also observed despite the presence of alpha-crystallin (which has anti-aggregating properties) in the system. Self-aggregation of the oxidized betaL-crystallin fragments per se was not observed under the experimental conditions. Reverse-phase HPLC analysis of the precipitate obtained after heating a mixture of betaL-crystallin and oxidized betaL-crystallin fragments revealed that more than one peptide co-precipitates with betaL-crystallin. Electrospray mass spectrometry analysis of the peptides revealed that the molecular weight(s) of the peptides ranged from 1400-1800. Tandem mass spectrometry and a data base search revealed that two of the peptides originated from betaA4-crystallin (LTIFEQENFLGR, residues 121-132) and betaB3-crystallin (AINGTWVGYEFPGYR, residues 153-167) respectively. Oxidized synthetic peptides representing the same sequence were also found to enhance the aggregation of betaL-crystallin in a manner similar to oxidized lens betaL-crystallin peptides. These data suggest that the polypeptides generated after oxidation and proteolysis of betaL-crystallins interact with denaturing proteins and facilitate their aggregation and light scattering, thus behaving like anti-chaperones.  (+info)

Decreased heat stability and increased chaperone requirement of modified human betaB1-crystallins. (5/70)

PURPOSE: To determine how deamidation and partial loss of the N- and C-terminal extensions alter the heat stability of betaB1-crystallin. METHODS: Human lens betaB1, a deamidated betaB1, Q204E, and alphaA-crystallins were expressed. Truncated betaB1 was generated by proteolytic removal of part of its terminal extensions. The aggregation and precipitation of these proteins due to heating was monitored by circular dichroism and light scattering. The effect of heat on the stability of both monomers and oligomers was investigated. The flexibility of the extensions in wild type and deamidated betaB1 was assessed by 1H NMR spectroscopy. RESULTS: With heat, deamidated betaB1 precipitated more readily than wild type betaB1. Similar effects were obtained for either monomers or oligomers. Flexibility of the N-terminal extension in deamidated betaB1 was significantly reduced compared to the wild type protein. Truncation of the extensions further increased the rate of heat-induced precipitation of deamidated betaB1. The presence of the molecular chaperone, alphaA-crystallin, prevented precipitation of modified betaB1s. More alphaA was needed to chaperone the truncated and deamidated betaB1 than deamidated betaB1 or truncated betaB1. CONCLUSIONS: Deamidation and truncation of betaB1 led to destabilization of the protein and decreased stability to heat. Decreased stability of lens crystallins may contribute to their insolubilization and cataract formation.  (+info)

A nonsense mutation in CRYBB1 associated with autosomal dominant cataract linked to human chromosome 22q. (6/70)

Autosomal dominant cataract is a clinically and genetically heterogeneous lens disorder that usually presents as a sight-threatening trait in childhood. Here we have mapped dominant pulverulent cataract to the beta-crystallin gene cluster on chromosome 22q11.2. Suggestive evidence of linkage was detected at markers D22S1167 (LOD score [Z] 2.09 at recombination fraction [theta] 0) and D22S1154 (Z=1.39 at theta=0), which closely flank the genes for betaB1-crystallin (CRYBB1) and betaA4-crystallin (CRYBA4). Sequencing failed to detect any nucleotide changes in CRYBA4; however, a G-->T transversion in exon 6 of CRYBB1 was found to cosegregate with cataract in the family. This single-nucleotide change was predicted to introduce a translation stop codon at glycine 220 (G220X). Expression of recombinant human betaB1-crystallin in bacteria showed that the truncated G220X mutant was significantly less soluble than wild type. This study has identified the first CRYBB1 mutation associated with autosomal dominant cataract in humans.  (+info)

BetaB1-crystallin: identification of a candidate ciliary body uveitis antigen. (7/70)

PURPOSE: Perineuclear anti-neutrophil cytoplasmic antibody (pANCA), a marker antibody present in 12% of patients with anterior uveitis, recognizes cytoplasmic antigens in the nonpigmented ciliary body epithelium, a probable site of immunologic reactivity in this inflammatory disease. In this study, a recombinantly isolated pANCA monoclonal antibody was used to identify the corresponding antigenic target(s) in the ciliary body. METHODS: Proteins from microdissected eye bank ocular ciliary body tissue were used to identify the corresponding ANCA antigen. Parallel two-dimensional protein gels were used for simultaneous identification of candidate antigenic protein spots by Western blot analysis and as a source of material for proteomic analysis. Multiple independent methods including Western blot analysis, confocal microscopy, and RT-PCR were used to provide additional characterization of the candidate protein. RESULTS: Proteomic analysis suggested that beta B1 (betaB1)-crystallin is the primary ciliary body antigen. The presence of betaB1-crystallin in the human ciliary body was confirmed by Western blot with a betaB1 specific anti-peptide antibody. Confocal microscopy revealed colocalization of the antigenic reactivity of both anti-betaB1 antibody and monoclonal pANCA. RT-PCR confirmed the presence of betaB1-crystallin RNA in the ciliary body tissues. CONCLUSIONS: This study identified betaB1-crystallin as a new cytoplasmic ciliary body antigenic target of a marker autoantibody associated with uveitis. This characterization of betaB1-crystallin outside the lens raises questions about its extralenticular expression, intracellular role, and potential target of inflammation in uveitis.  (+info)

Improving the performance of DomainParser for structural domain partition using neural network. (8/70)

Structural domains are considered as the basic units of protein folding, evolution, function and design. Automatic decomposition of protein structures into structural domains, though after many years of investigation, remains a challenging and unsolved problem. Manual inspection still plays a key role in domain decomposition of a protein structure. We have previously developed a computer program, DomainParser, using network flow algorithms. The algorithm partitions a protein structure into domains accurately when the number of domains to be partitioned is known. However the performance drops when this number is unclear (the overall performance is 74.5% over a set of 1317 protein chains). Through utilization of various types of structural information including hydrophobic moment profile, we have developed an effective method for assessing the most probable number of domains a structure may have. The core of this method is a neural network, which is trained to discriminate correctly partitioned domains from incorrectly partitioned domains. When compared with the manual decomposition results given in the SCOP database, our new algorithm achieves higher decomposition accuracy (81.9%) on the same data set.  (+info)

View mouse Crybb3 Chr5:113075839-113081584 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Gene target information for Crybb1 - crystallin, beta B1 (house mouse). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Buy our Recombinant Human beta Crystallin protein. Ab117029 is a full length protein produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE…
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References for Abcams Recombinant Human Beta crystallin S protein (ab111642). Please let us know if you have used this product in your publication
Purpose: The purpose of this study was to investigate the impact of three amino acid changes caused by a CRYBB2 gene conversion event that we identified in a large congenital cataract family.. Methods: The CRYBB2 cDNA was generated by RT-PCR using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems), RNA from human retina, CRYBB2-specific primers and the pcDNA3.1 vector. Three mutations, c.433 C,T (p.R145W), c.440A,G (p.Q147R), and c.449C,T (p.T150M), were introduced into the wild type clone using the QuikChange Lightning kit (Agilent). Cultured human lens epithelium (HLE) SRA 01/04 cells were transfected using Lipofectamine 2000 (Life Technologies). Cell lysates containing protease inhibitors were sonicated and spun to generate separate supernatant and pellet fractions. After SDS-PAGE, Western blot analysis was carried out using a goat polyclonal antibody against CRYBB2 (Santa Cruz Biotechnology).. Results: Missense changes R145W and T150 M were found to be conserved across 15 ...
Abstract: : Purpose: Pax6, Prox1 and Mafs are transcription factors important for crystallin gene expression and eye development. We previously showed that, in transfection assays, MafB and Prox1 could synergistically activate the chicken betaB1 crystallin promoter (-432/+30), however, both MafB and Prox1 mediated transactivation could be blocked when Pax6 was co-transfected. This study investigates how these transcription factors cooperate to regulate betaB1-crystallin gene expression. Methods: Distribution of MafB was investigated in mouse lens and lens cell lines by immunohistochemistry and western blotting. GST-tagged MafB, Prox1 and Pax6 were generated by using pET or pGEX recombinant protein expression system. Their DNA binding abilities were tested in electrophoresis mobility shift assays (EMSA). Results: MafB was differentially expressed in lens fiber cells and lens epithelial cells: in lens epithelial cells, MafB is not evenly distributed in nuclei but concentrated in a single large ...
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Reaktivität: Rind (Kuh), Hund, Meerschweinchen and more. 21 verschiedene CRYZ Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Congenital cataract - MedHelps Congenital cataract Center for Information, Symptoms, Resources, Treatments and Tools for Congenital cataract. Find Congenital cataract information, treatments for Congenital cataract and Congenital cataract symptoms.
Purified anti-Alpha Crystallin B - read details of BioLegend antibodies in the SelectScience.net Antibody products and suppliers directory
The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.
ICD-9 code 743.30 for Congenital cataract unspecified is a medical classification as listed by WHO under the range - CONGENITAL ANOMALIES (740-759).
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Although multidomain proteins predominate the proteome of all organisms and are expected to display complex folding behaviors and significantly greater structural dynamics as compared with single-domain proteins, their conformational heterogeneity and its impact on their interaction with ligands are poorly understood due to a lack of experimental techniques. The multidomain calcium-binding βγ-crystallin proteins are particularly important because their deterioration and misfolding/aggregation are associated with melanoma tumors and cataracts. Here we investigate the mechanical stability and conformational dynamics of a model calcium-binding βγ-crystallin protein, Protein S, and elaborate on its interactions with calcium. We ask whether domain interactions and calcium binding affect Protein S folding and potential structural heterogeneity. Our results from single-molecule force spectroscopy show that the N-terminal (but not the C-terminal) domain is in equilibrium with an alternative ...
We have investigated the gene expression, effect of deletion from the genome, and the immune response to Acr2, a novel α-crystallin of MTB. MTB has two members of the α-crystallin family of molecular chaperones. Although neither is absolutely required for growth in vivo or in vitro (32), Acr1 has been extensively characterized in the context of its role in the bacterial response to hypoxia/NO and as an immunodominant Ag. Our study demonstrates that Acr2 also makes a contribution to pathogen biology but more so to the host immune response. We found that acr2 gene expression was up-regulated shortly after entry into cells in response to exposure to host reactive oxygen. As a consequence, Acr2 is a prominent immune target, especially of T cells in individuals who are asymptomatic but recently exposed to infectious tuberculosis.. Expression of the acr2 gene is controlled by the heat shock regulator, HspR (5), and by the alternative ς factor, ςE, which is itself regulated by heat shock and by ...
Crystallins are water-soluble proteins that compose about ninety% in the protein inside the lens.[13] The three main crystallin sorts present in the human eye are α-, β-, and γ-crystallins. Crystallins have a tendency to variety soluble, high-molecular pounds aggregates that pack tightly in lens fibers, Therefore raising the index of refraction of the lens while protecting its transparency. β and γ crystallins are found mostly during the lens, while subunits of α -crystallin happen to be isolated from other areas of the eye and the human body ...
Creative Biolabs has established a platform predict and assess deamidation of antibody therapeutics as part of manufacturability assessment.
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Isolation and characterization of a gene expressed mainly in the gastric epithelium, a novel member of the ep37 family that belongs to the βγ-crystallin superfamily (1998 ...
Congenital cataract, although uncommon, accounts for about 10% of childhood blindness.1 The cataract is usually seen as an isolated abnormality but may occur in association with other ocular developmental or systemic abnormalities. About 50% of bilateral cases have a genetic basis. Congenital cataract is both clinically and genetically heterogeneous; isolated congenital cataract is usually inherited as an autosomal dominant trait although autosomal recessive and X linked inheritance are seen less commonly.2 Most progress has been made in identifying the genes causing autosomal dominant congenital cataract.2 Two main approaches have been used to identify the causative mutations. In large families linkage analysis has been used to identify the chromosomal locus followed by screening of positional candidate genes; most genes have been identified using this strategy. A second approach has been to screen DNA from large panels of patients with inherited cataract for mutation in the many candidate ...
TY - JOUR. T1 - βA3/A1-crystallin is required for proper astrocyte template formation and vascular remodeling in the retina. AU - Sinha, Debasish. AU - Valapala, Mallika. AU - Bhutto, Imran. AU - Patek, Bonnie. AU - Zhang, Cheng. AU - Hose, Stacey. AU - Yang, Fang. AU - Cano, Marisol. AU - Stark, Walter J.. AU - Lutty, Gerard A.. AU - Zigler, J. Samuel. AU - Wawrousek, Eric F.. PY - 2012/9/1. Y1 - 2012/9/1. N2 - Nuc1 is a spontaneous rat mutant resulting from a mutation in the Cryba1 gene, coding for βA3/A1-crystallin. Our earlier studies with Nuc1 provided novel evidence that astrocytes, which express βA3/A1-crystallin, have a pivotal role in retinal remodeling. The role of astrocytes in the retina is only beginning to be explored. One of the limitations in the field is the lack of appropriate animal models to better investigate the function of astrocytes in retinal health and disease. We have now established transgenic mice that overexpress the Nuc1 mutant form of Cryba1, specifically in ...
Results. Effect of [alpha]-crystallin on restriction enzyme digestion. To determine whether the partially purified [alpha]-crystallin had inhibitory effects on restriction digestion of DNA, we assayed several commonly used restriction enzymes for activity in the presence of [alpha]-crystallin. The commonly used restriction enzymes, Bam HI, Hind III, Nde I, Pst I and Sst I all remained active in the presence of [alpha]-crystallin. For this experiment, a 5 fold excess of each enzyme (5 units) was incubated for 1 hour with 1 µg of plasmid DNA using manufacturers supplied buffer. Each reaction also contained 1 µl of the purified [alpha]-crystallin (10 mg/ml), final concentration, 1 mg/ml. Each selected enzyme digested the plasmid to completion indicating that [alpha]-crystallin had no major detrimental effects on restriction enzyme digestion (data not shown).. To assay the ability of [alpha]-crystallin to protect restriction enzymes from heat inactivation, we chose the enzyme Nde I. Unlike the ...
In the current study, we confirmed a missense mutation c. 139 G , A in Cx50 (GJA8) in a six-generation Chinese pedigree with congenital cataract. This mutation resulted in an asparagine substitution for aspartic at amino acid residue 47 (D47N).. Cataracts are defined as opacification of the normally transparent crystalline lens, and are the leading cause of vision loss in the world. Congenital cataract is a type of cataract that emerges at birth or during early childhood [5, 18]. The abnormality of lens can interfere with normal development of eyes [5, 19]. Congenital cataracts can be inherited or familial, either as an isolated lens phenotype or as part of a genetic/metabolic disorder, commonly with full penetrance and autosomal dominant transmission [19]. Genetic factors play an important role in congenital cataract [20]. Gene mutations that affecting the lens development during embryonic period are considered to be the main cause [18]. Up to now, more than 39 genes and loci have been ...
A quantitative analysis of cell division and cell elongation was carried out during lens morphogenesis in the rat. At 13 days of development elongating cells in the posterior part of the lens vesicle (presumptive fibre cells) have a lower mitotic activity than cells in the anterior vesicle. By 14 days these elongating cells do not divide. Thus at 14 days of development the lens can be separated into two compartments; a proliferation compartment in the anterior lens and an elongation compartment in the posterior lens.. The three main groups of lens-specific proteins, α-,β- and γ-crystallins, were localized by immunofiuorescence. Alpha-crystallin is the first crystallin to be detected and is localized in some lens pit cells at 12 days of development. By 14 days all lens cells contain α-crystallin. Beta- and β-crystallins are detected later at 12½ days and are localized in some cells situated primarily in the posterior part of the lens vesicle. At later stages of development these crystallins ...
Discussion. The aims of the present study were to comparatively identify parent crystallins of the crystallin fragments present in WS-α-crystallin, WS-HMW, and WI protein fractions of human cataractous (with nuclear opacity) and age matched normal lenses, and to identify post-translational modifications in crystallin species present in the WS-HMW and WI protein fractions of cataractous lenses. The rationale was that the results would distinguish cataract specific aggregated crystallin species from those that aggregate during aging in both WS-HMW and WI protein fractions.. The major findings of this study are: (1) the majority of aggregated species in the WS-HMW and in the WI protein fractions during aging and nuclear cataract development were fragments of αA- and αB-crystallin, (2) while the younger lenses contained only the C-terminally truncated αA- and αB-crystallin, the older normal lenses showed age related truncation of both N- and C- terminal regions in the crystallins, (3) in the ...
The major components of the lens are highly stable and water-soluble crystallin proteins. Once deposed, the proteins remain in the lens and create a structure providing unique transparency and accommodation properties [12]. In spite of the high stability, molecular changes accumulate in the proteins over time caused by chemical, photochemical, environmental factors or by ageing and concomitant diseases, e.g. diabetes [13,14]. The changes lead to protein misfolding, denaturation and aggregation and hence structural defects [15]. Currently, cataract diagnostics based on light scattering only detect defects comparable in size to the wavelength so that detection of the impact of photochemical change is hardly possible at the stage when a cataract is currently diagnosed. Thus, surgical treatment is the only currently possible method.. Although cataract surgery is generally recognized as being one of the safest operations, and there is a significant complication rate [16]. Furthermore, there is ...
de Jong, W.W., Zweers, A. and Goodman, M. (1981) Relationship of Aardvark to Elephants, Hyraxes and Sea Cows from α-Crystallin Sequences. Nature, 292, 538-540.
During development of the mammalian eye, nourishment of the immature lens, inner retina and vitreous is provided by the hyaloid vascular system. One of the cong...
Cataracts present from birth (congenital cataracts) are sometimes caused by a faulty gene being passed to a child from their parents. This fault means that the lens doesnt develop properly.. Its estimated that theres a family history of congenital cataracts in around one in every five cases of the condition, and recent research suggests genetic causes are responsible for the majority of bilateral congenital cataracts in the UK.. Cataracts can also be associated with conditions caused by chromosome abnormalities, such as Downs syndrome. Chromosomes are the parts of the bodys cells that carry the genes. ...
The long-term objective of this proposal is to determine the molecular mechanisms by which modifications of the ?-crystallins (genetic or age-onset), lead to ca...
When a baby is born with cataracts or develops them at a very early age, the condition is called congenital or infantile cataracts. A cataract can make your vision blurry, like trying to look through frosted glass.
Human ζ-crystallin is a Zn(2+)-lacking medium-chain dehydrogenase/reductase (MDR) included in the quinone oxidoreductase (QOR) family because of its activity with quinones. In the present work a novel enzymatic activity was characterized: the double bond α,β-hydrogenation of medium-chain 2-alkenals and 3-alkenones. The enzyme is especially active with lipid peroxidation products such as 4-hydroxyhexenal, and a role in their detoxification is discussed. This specificity is novel in the QOR family, and it is similar to that described in the distantly related alkenal/one reductase family. Moreover, we report the X-ray structure of ζ-crystallin, which represents the first structure solved for a tetrameric Zn(2+)-lacking MDR, and which allowed the identification of the active-site lining residues. Docking simulations suggest a role for Tyr53 and Tyr59 in catalysis. The kinetics of Tyr53Phe and Tyr59Phe mutants support the implication of Tyr53 in binding/catalysis of alkenal/one substrates, while Tyr59 is
Inhibin beta B antibody [C3], C-term (inhibin, beta B) for ICC/IF, IHC-P, WB. Anti-Inhibin beta B pAb (GTX108408) is tested in Human samples. 100% Ab-Assurance.
11- Zafaranchi zadeh moghadam S, Khoshaman K, Masoudi R, Hemmateenejad B, Yousefi R (2017) "The structural alteration and aggregation propensity of glycated lens crystallins in the presence of calcium; importance of lens calcium homeostasis in development of diabetic cataracts. Spectrochimica Acta Part A: Molec " Spectrochim Acta A Mol Biomol Spectroscopy 170:174-183 ...
Expression of insoluble protein - posted in Protein Expression and Purification: Hi AllI am facing a serious problem in expression of a protein in soluble fractionI have a full-length gene (approx. 1.6kb) cloned in pExp5 TOPO/ CT vector.Sequencing of the clones revealed that the gene is in-frame. I have expressed it in Rosetta-BL21(DE3)pLys-S cells at 18 C in an autoinduction media. I could see the clear expression in lysed cell pellate (~64KDa) when SDS-PAGE was done, whereas the final el...
Learn about the causes, symptoms, diagnosis & treatment of Eye Disorders in Children from the Home Version of the Merck Manuals.
CRYM antibody [1G7] (crystallin, mu) for FACS, ICC/IF, IHC-P, WB. Anti-CRYM mAb (GTX84654) is tested in Human, Rat samples. 100% Ab-Assurance.
Author Kristin Newman shares a few of her favourite ways to spend a day near Four Seasons destinations, from Los Angeles to Chiang Mai and beyond.
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... de Jong WW, Terwindt EC, Bloemendal H (1976). "The amino acid sequence of the A chain of human alpha-crystallin". FEBS Lett. 58 ...
... alpha-crystallin b chain MeSH D12.776.306.366.300.100 - beta-crystallin a chain MeSH D12.776.306.366.300.200 - beta-crystallin ... myosin heavy chains MeSH D12.776.210.500.600.200 - myosin light chains MeSH D12.776.210.500.600.300 - myosin subfragments MeSH ... myosin heavy chains MeSH D12.776.220.525.475.200 - myosin light chains MeSH D12.776.220.525.475.300 - myosin subfragments MeSH ... alpha-crystallin a chain MeSH D12.776.306.366.100.300 - ... beta-2 microglobulin MeSH D12.776.377.715.182.160 - beta- ...
"Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2 ... This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B3. A chain-terminating ... "Linkage between the beta B2 and beta B3 crystallin genes in man and rat: a remnant of an ancient beta-crystallin gene cluster ... Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. It is part of the small heat shock protein ... Alpha B chain crystallins (αBC) can be induced by heat shock, ischemia, and oxidation, and are members of the small heat shock ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ...
... rho crystallin, and many others. All possess a similar structure, with a beta-alpha-beta fold characteristic of nucleotide ... Some proteins of this family contain a potassium channel beta chain regulatory domain; these are reported to have ... The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being ... Gulbis JM, Zhou M, Mann S, MacKinnon R (July 2000). "Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent ...
... alpha 1 Crystallin, beta A1 Cyclic nucleotide-gated channel alpha 1 Cyclin A1 Cytochrome P450, family 1, member A1 Defensin, ... a portion of the cholera toxin chain alleles A1, an allele in the DRD2 TaqI polymorphism that could be involved in alcoholism. ... DQ alpha 1 Myosin light chain A1, an actin-binding protein NADH dehydrogenase (ubiquinone), alpha 1 Nucleolar protein, member ...
The yeast killer toxin structure was thought to be a precursor of the two-domain beta gamma-crystallin proteins, because of its ... is a double-chain inhibitor consisting of an 11-residue and a 41-residue chain. The Carboxypeptidase inhibitor I68 family ... with two alpha-helices and four beta-strands with a (beta/alpha/beta)x2 topology. The peptidase inhibitor I9 family contains ... SMPI is composed of two beta-sheets, each consisting of four antiparallel beta-strands. The structure can be considered as two ...
Crystallin zeta CYP4B1 (1p33) CYR61 (1p22) DBT: dihydrolipoamide branched chain transacylase E2 DCLRE1B: DNA cross-link repair ... beta; acid (includes glucosylceramidase) (gene for Gaucher disease) GBAP1: glucosylceramidase beta pseudogene 1 GLC1A: gene for ... beta 3, 31kDa (connexin 31) GLMN (1p22) GNL2: G protein nucleolar 2 GSTM1 (1p13) HDAC1 (1p35) HES2: Hes family bHLH ... heavy chain 14 ECM1 (1q21) EDEM3: ER degradation enhancing alpha-mannosidase like protein 3 EGLN1 (1q42) ENAH (1q42) ESRRG ( ...
CRYZ: Crystallin zeta. *CYP4B1 (1p33). *CYR61 (1p22). *DBT: dihydrolipoamide branched chain transacylase E2 ... GBA: glucosidase, beta; acid (includes glucosylceramidase) (gene for Gaucher disease). *GBAP1: glucosylceramidase beta ... ACADM: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain ... GJB3: gap junction protein, beta 3, 31kDa (connexin 31). *GLMN ...
4-beta-glucosidase MeSH D08.811.277.450.420.200.600 --- glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 --- ... cholesterol side-chain cleavage enzyme MeSH D08.811.600.250 --- electron transport chain complex proteins MeSH D08.811.600.250. ... delta-crystallins MeSH D08.811.520.232.400 --- ammonia-lyases MeSH D08.811.520.232.400.200 --- aspartate ammonia-lyase MeSH ... 4-beta xylanases MeSH D08.811.277.450.950.500 --- xylan endo-1,3-beta-xylosidase MeSH D08.811.277.656.149 --- atp-dependent ...
The N-terminal domain I (aa ~1-195) contains attachment sites for HS chains. Although HS chains are not required for correct ... This role for perlecan in beta-amyloid plaque generation is supported by an earlier study showing that perlecan and beta- ... To this end, a transgenic mouse line was created expressing porcine TGF-β1 under the lens-specific αA-crystallin promoter and ... The heparan sulfate chains of perlecan bind growth factors in the ECM, and serve as co-ligands or ligand enhancers when bound ...
"Myxococcus xanthus spore coat protein S may have a similar structure to vertebrate lens beta gamma-crystallins". Nature. 315 ( ... However, these binding sites were based on a cluster analysis of side-chain oxygen atoms and on results from site-directed ... "Structural similarity of a developmentally regulated bacterial spore coat protein to beta gamma-crystallins of the vertebrate ... The domains share high similarity with other crystallin proteins. Protein S binds two 2 mol of calcium per mol of protein with ...
δ-crystallins are the major structural eye lens water-soluble proteins of most birds, reptiles, and some other vertebrates. ... Domain 2 contains one small beta sheet, nine alpha helices, and the carboxyl terminus. Three of the nine alpha helices on one ... ASL is composed of four identical monomers; each monomer consisting of a single polypeptide chain between 49 and 52 kDa, ... ASL, δ-crystallin, class II fumarase, aspartase, adenylosuccinase lyase, and 3-carboxy-cis and cis-muconate lactonizing enzyme ...
Beta cells (secreting insulin and amylin) Delta cells (secreting somatostatin) PP cells (gamma cells) (secreting pancreatic ... Intermediate skeletal muscle cell Nuclear bag cell of muscle spindle Nuclear chain cell of muscle spindle Satellite cell (stem ... Anterior lens epithelial cell Crystallin-containing lens fiber cell Adipocytes: White fat cell Brown fat cell Liver lipocyte ...
Silva, R.A.G.D., *Nguyen, J.Y., and Decatur, S.M. (2002) "Probing the Effects of Side Chains on the Conformation and Stability ... Phi Beta Kappa (elected 1990) Sigma Xi (elected 1990) Op-ed pieces on higher education "The Winner - A Liberal Education," New ... "Formation of Amyloid Fibrils in Vitro by Human γd-crystallin and its Isolated Domains," Molecular Vision 14:81-89. Decatur, S.M ... "Twodimensional IR Spectroscopy and Segmental 13C Labeling reveals the Domain Structure of Human γδ-crystallin amyloid fibrils ...
Konrad RJ, Kudlow JE (Nov 2002). "The role of O-linked protein glycosylation in beta-cell dysfunction". International Journal ... and a peptide chain with a reactive serine or threonine hydroxyl group. The proposed reaction is an ordered sequential bi-bi ... "Vertebrate lens alpha-crystallins are modified by O-linked N-acetylglucosamine". The Journal of Biological Chemistry. 267 (1): ... Hart GW, Housley MP, Slawson C (Apr 2007). "Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins". Nature ...
Oligomerization is a chemical process that converts individual molecules into a chain consisting of a finite number of ... "Monitoring the prevention of amyloid fibril formation by alpha-crystallin. Temperature dependence and the nature of the ... Amyloid beta can be measured semiquantitatively with immunostaining, which also allows one to determine location. Amyloid beta ... By NMR-guided simulations, amyloid beta 1-40 and amyloid beta 1-42 also seem to feature highly different conformational states ...
Iodine (I0) is very reactive and iodinates the thyroglobulin at tyrosyl residues in its protein chain (in total containing ... However, recently it could be demonstrated that the crystallin CRYM binds 3,5,3′-triiodothyronine in vivo.[37] ... "Genome-wide binding patterns of thyroid hormone receptor Beta". PLOS One. 9 (2): e81186. Bibcode:2014PLoSO...981186A. doi ... "μ-Crystallin as an Intracellular 3,5,3′-Triiodothyronine Holder in Vivo". Molecular Endocrinology. 21 (4): 885-894. doi ...
Wang P, Gao H, Ni Y, Wang B, Wu Y, Ji L, Qin L, Ma L, Pei G (February 2003). "Beta-arrestin 2 functions as a G-protein-coupled ... Wang P, Wu Y, Ge X, Ma L, Pei G (March 2003). "Subcellular localization of beta-arrestins is determined by their intact N ... In addition, MDM2 has p53-independent transcription factor-like effects in nuclear factor-kappa beta (NFκB) activation. ... Long-chain-fatty-acid-CoA ligase. 6.3: Carbon-Nitrogen. *Glutamine synthetase. *Ubiquitin ligase *Cullin ...
α-crystallin, a process which may be sped up by higher temperatures.[19][20] ... The authors propose that mtDNA mutations lead to respiratory-chain-deficient cells and thence to apoptosis and cell loss. They ... "Age-Related Loss of Innate Immune Antimicrobial Function Of Dermal Fat Is Mediated By Transforming Growth Factor Beta" ... the activity levels of the electron transport chain in mitochondria[57] and (in plants) in chloroplasts. ...
Truncated human betaB1-crystallin shows altered structural properties and interaction with human betaA3-crystallin. ... The purpose of the study was to determine the effects of truncation of various regions of betaB1-crystallin on its structural ... properties and stability of heterooligomers formed by wild-type (WT) betaB1 or its deletion mutants with WT betaA3-crystallin. ...
Mouse Monoclonal Anti-AlphaA Crystallin/CRYAA Antibody (1H3.B8). Validated: WB, ELISA, IB, ICC/IF. Tested Reactivity: Human. ... Heat shock protein beta-4. *HSPB4. *HSPB4alpha-crystallin A chain. *human alphaA-crystallin (CRYA1), 10HspB4 ... Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins. ... Home » AlphaA Crystallin/CRYAA » AlphaA Crystallin/CRYAA Antibodies » AlphaA Crystallin/CRYAA Antibody (1H3.B8) ...
Crystallin Beta A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Beta Crystallin A3 Chain Transcript LAM 3 * Beta Crystallin A3 Chain Transcript CN 3 ... crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 ... Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ...
Suppression of thermal aggregation of beta-L-crystallin by alpha-crystallin. Biochemistry 2005, 44, 15480-15487. [Google ... These light chains are nearly identical except for first 41 additional amino acid residues of the A1 light chain, containing ... Interaction of the N-terminal part of the A1 essential light chain with the myosin heavy chain. Biochem. Biophys. Res. Commun ... also called alkali light chain [4]. In fast skeletal muscle myosin there are two kinds of alkali light chains, A1 and A2. ...
Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2. ... Three main families of mammalian crystallins are α-, β-, and γ-crystallins. α-Crystallins (αA and αB) are homologous to small ... Crystallins of the beta/gamma-superfamily mimic the effects of lens injury and promote axon regeneration. Mol Cell Neurosci. ... The evolution of lenticular proteins: the beta- and gamma-crystallin super gene family. Prog Biophys Mol Biol. 1988;51:47-76. [ ...
Chain. 2 - 174. Gamma-crystallin D. Domain. 88 - 128. Beta/gamma crystallin Greek key 3. ... Structural and evolutionary relationships among five members of the human gamma-crystallin gene family. Meakin S.O.; Breitman M ... Novel mutations in the gamma-crystallin genes cause autosomal dominant congenital cataracts. Santhiya S.T.; Shyam Manohar M.; ...
Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Crystallins are separated into two classes: ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ... de Jong WW, Terwindt EC, Bloemendal H (1976). "The amino acid sequence of the A chain of human alpha-crystallin". FEBS Lett. 58 ...
... wherein a rearrangement of the amino terminal of the beta-chain of hemoglobin by reaction with glucose, forms the adduct known ... This reaction was also found to occur with a variety of other body proteins, such as lens crystallins, collagen and nerve ... The preferred are peroxidase, beta-glucuronidase, beta-D-glucosidase, beta-D-galactosidase, urease, glucose oxidase plus ... The indicator composition of claim 14 wherein said label is selected from peroxidase, beta-glucuronidase, beta-D-glucosidase, ...
Rat A crystallin A Monoclonal Antibody (Clone c9F2)-NP_000385 (MBS200007) product datasheet at MyBioSource, Primary Antibodies ... Heat shock protein beta-4; HspB4; Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1 ... Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene ... This antibody is not shown cross-activity about Crystallin alpha B. A : Crystallin-alpha A recombinant protein B : Crystallin- ...
Alpha crystallin B chain; Alpha crystallin B chain; Alpha(B) crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CRYA2; ... Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ... β5/α ״???? B/αB-crystallin ??. ??. alpha B Crystallin (phospho S53); alpha B Crystallin (phospho Ser53); p-alpha B Crystallin ( ... Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four ...
Rabbit polyclonal alpha A Crystallin/CRYAA antibody. Validated in WB, IHC, BL, sELISA and tested in Mouse, Rat, Cow. Cited in ... The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the ... O-glycosylated; contains N-acetylglucosamine side chains.. Deamidation of Asn-101 in lens occurs mostly during the first 30 ... There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at ...
Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2. ... A unique form of autosomal dominant cataract explained by gene conversion between beta-crystallin B2 and its pseudogene. J Med ... The γ-crystallin gene cluster on chromosome 2q33-35 encompasses genes γA to D but only γC (CRYGC) and γD(CRYGD) are highly ... Heon E, Priston M, Schorderet DF, et al. The gamma-crystallins and human cataracts: a puzzle made clearer. Am J Hum Genet 1999; ...
T-cell receptor beta-chain (38), 2-crystallin (39), 1 tubulin The functional and physical unit of heredity passed from parent ... T-cell receptor beta-chain enhancer: the role of enhancer elements in controlling V(D)J recombination accessibility. Proc Natl ... differentiation stage-specific expression of the rat beta B2-crystallin The functional and physical unit of heredity passed ... differentiation stage-specific expression of the rat beta B2-crystallin The functional and physical unit of heredity passed ...
Rabbit polyclonal Alpha B Crystallin (phospho S19) antibody validated for WB, IHC, ICC/IF and tested in Human, Mouse and Rat. ... Alpha crystallin B chain antibody. *Alpha(B)-crystallin antibody. *Alpha-crystallin B chain antibody ... Heat shock protein beta 5 antibody. *Heat shock protein beta-5 antibody ... Lane 1 : phospho-alpha-B crystallin (Ser19). Lane 2 : unphosphorylated alpha-B crystallin. Observed band size : 20 kDa (why is ...
Linkage of the locus encoding the A chain of alpha-crystallin (Acry-1) to the major histocompatibility complex in the rat. Skow ... Hematology of a murine beta-thalassemia: a longitudinal study. Popp RA, Popp DM, Johnson FM, Skow LC, Lewis SE. Ann N Y Acad ... Polymorphism and linkage of the alpha A-crystallin gene in t-haplotypes of the mouse. Skow LC, Nadeau JN, Ahn JC, Shin HS, ... The locus encoding alpha A-crystallin is closely linked to H-2K on mouse chromosome 17. Skow LC, Donner ME. Genetics. 1985 110( ...
"Autosomal dominant cerulean cataract is associated with a chain termination mutation in the human beta-crystallin gene CRYBB2 ... This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B3. A chain-terminating ... "Linkage between the beta B2 and beta B3 crystallin genes in man and rat: a remnant of an ancient beta-crystallin gene cluster ... Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as ...
Purified anti-Alpha Crystallin B - read details of BioLegend antibodies in the SelectScience.net Antibody products and ... Alpha-crystallin B chain, alpha B crystallin, heat shock protein beta-5, rosenthal fiber component, heat-shock 20 kD like- ... Purified anti-Alpha Crystallin B. Be the first to review this product ... Purified anti-Alpha Crystallin B; Clone: Poly9079; Reactivity: Human, Mouse; Apps: IHC, WB; Size: 200 μl ...
... non-glycosylated polypeptide chain containing 216 amino acids (1-196 a.a.). ... Beta-crystallin A4 (CRYBA4) is a member of the beta/gamma-crystallin family which are the dominant structural components of the ... The CRYBA4 gene being a beta acidic group member is part of a gene cluster with beta-B1, beta-B2, and beta-B3. ... Beta-crystallins construct aggregates of various sizes and are able to self-associate to form dimers or to form heterodimers ...
... λ and κ L chains, and albumin, whereas albumin, IgG1 H chain, and λ and κ L chains were observed in samples from normal sera ( ... Antibodies to myelin basic protein, myelin oligodendrocytes peptides, alpha-beta-crystallin, lymphocyte activation and cytokine ... Crystal structures of alpha-crystallin domain dimers of alphaB-crystallin and Hsp20. J. Mol. Biol. 392: 1242-1252. ... Natural beta-sheet proteins use negative design to avoid edge-to-edge aggregation. Proc. Natl. Acad. Sci. USA 99: 2754-2759. ...
Alpha-crystallin A chain. Alpha-crystallin A chain (Heat shock protein beta-4, HspB4) [Cleaved into: Alpha-crystallin A(1-172) ... Beta-ketoacyl reductase) ; 3-oxoacyl-[acyl-carrier-protein] synthase, EC 2.3.1.41 (Beta-ketoacyl synthase) ] ...
Genes of interest are cloned into the multiple cloning site of the Vector System just downstream of the p26 or alpha crystallin ... A method for expressing proteins as a fusion chimera with a domain of p26 or alpha crystallin type proteins to improve the ... As used herein the active anti-parallel beta sheets is defined by amino acid residues 36-160. As used herein the term " ... Hayhurst, A., "Improved Expression Characteristics of Single-Chain Fv Fragments When Fused Downstream of the Escherichia coli ...
beta-Crystallin A Chain / genetics Actions. * Search in PubMed * Search in MeSH ... Genetics of crystallins: cataract and beyond. Graw J. Graw J. Exp Eye Res. 2009 Feb;88(2):173-89. doi: 10.1016/j.exer.2008.10. ... Novel mutation in the gamma-S crystallin gene causing autosomal dominant cataract. Vanita V, Singh JR, Singh D, Varon R, ... A novel MAF missense mutation leads to congenital nuclear cataract by impacting the transactivation of crystallin and ...
beta-Crystallin A Chain / genetics* Actions. * Search in PubMed * Search in MeSH ... Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus Daniel W H ... Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus Daniel W H ... Based on initial and replication studies, rs2009066 located in the crystallin beta A4 (CRYBA4) gene was identified to be the ...
... beta-myosin heavy chain was upregulated (+1.7). In the RA, 22 proteins spots were altered including the following downregulated ... Crystallin alpha B (hypertrophy inhibitor) was upregulated in both the RV (+2.2) and RA (+2.6).In early stage hypertrophy there ... Downregulated RV proteins included contractile elements: troponin T and C (-1.6 fold change), myosin regulatory light chain 2 ... proteins contractile elements: tropomyosin 1 alpha chain (-1.9), cellular energetic proteins: ATP synthase (-1.5), fatty-acid ...
  • Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM: (abcam.com)
  • We report here that UPS proteolytic function is severely impaired in the heart of a mouse model of intrasarcoplasmic amyloidosis caused by cardiac-restricted expression of a human desmin-related myopathy-linked missense mutation of αB-crystallin (CryAB R120G ). (ahajournals.org)
  • 10,11 Mice carrying 3 copies of a Tg consisting of the mouse α-myosin heavy chain promoter and the CryAB R120G cDNA show no apparent cardiac abnormality at 1 month but develop concentric cardiac hypertrophy with diastolic malfunction at 3 months and die of CHF between 5 and 7 months. (ahajournals.org)
  • This study clearly demonstrated that both the length and electrostatic charge of the COOH-terminal segment play crucial roles in governing the structural stability and chaperone activity of alphaB-crystallin . (antibodies-online.com)
  • We propose that these two interacting loops play an important role in the folding and structural stability of β/γ-crystallin domains by protecting the hydrophobic core from solvent access. (bvsalud.org)
  • Numerous cataract-causing mutations have been identified in various β/γ-crystallins, but the mechanisms underlying cataract caused by most mutations remains uncharacterized. (bvsalud.org)
  • The S228P mutation modified the refolding pathway of βB1-crystallin by affecting the formation of the dimeric intermediate but not the monomeric intermediate. (bvsalud.org)
  • 0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.Use in Immunoblotting reported in scientific literature (PMID 28546921). (novusbio.com)
  • Western blot analysis of whole cell lysates probed with alpha B crystallin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 4 second exposure. (bio-rad-antibodies.com)
  • S1 prepared by chymotryptic digestion of skeletal muscle myosin lacks the regulatory light chain but does contain the essential light chain, also called alkali light chain [ 4 ]. (mdpi.com)
  • In fast skeletal muscle myosin there are two kinds of alkali light chains, A1 and A2. (mdpi.com)
  • 1993. The t cell receptor alpha-beta v-j shuffling shows lack of autonomy between the combining site and the constant domain of the receptor chains. (springer.com)
  • Immunoglobulin heavy-chain enhancer is required to maintain transfected gamma 2A gene expression in a pre-B-cell line. (asm.org)
  • An increased αB-crystallin level has been associated with neurologic diseases, including Alexander's, Creutzfeldt-Jakob, and Parkinson's diseases. (arvojournals.org)
  • Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted. (abcam.com)