A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.
A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Discrete abnormal tissue masses that protrude into the lumen of the INTESTINE. A polyp is attached to the intestinal wall either by a stalk, pedunculus, or by a broad base.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A specific complex of WNT SIGNALING PATHWAY proteins that mediates the phosphorylation-dependent destruction of cytosolic BETA-CATENIN. The complex is disrupted by cell surface binding of WNT PROTEINS, which allows beta-catenin levels to rise to the point where they migrate to the CELL NUCLEUS and activate transcription.
A multi-functional catenin that is highly homologous to BETA CATENIN. Gamma catenin binds CADHERINS and helps link their cytoplasmic tails to ACTIN in the CYTOSKELETON via ALPHA CATENIN. It is also found in DESMOSOMES where it mediates the link between DESMOSOMAL CADHERINS and DESMOPLAKIN.
A hereditary disease caused by autosomal dominant mutations involving CHROMOSOME 19. It is characterized by the presence of INTESTINAL POLYPS, consistently in the JEJUNUM, and mucocutaneous pigmentation with MELANIN spots of the lips, buccal MUCOSA, and digits.
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
A malignant kidney tumor, caused by the uncontrolled multiplication of renal stem (blastemal), stromal (STROMAL CELLS), and epithelial (EPITHELIAL CELLS) elements. However, not all three are present in every case. Several genes or chromosomal areas have been associated with Wilms tumor which is usually found in childhood as a firm lump in a child's side or ABDOMEN.
A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.
Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
Adherence of cells to surfaces or to other cells.
A malignant epithelial tumor with a glandular organization.
One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.
An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
A family of proteins that contain several 42-amino acid repeat domains and are homologous to the Drosophila armadillo protein. They bind to other proteins through their armadillo domains and play a variety of roles in the CELL including SIGNAL TRANSDUCTION, regulation of DESMOSOME assembly, and CELL ADHESION.
Established cell cultures that have the potential to propagate indefinitely.
A single-pass transmembrane glycoproteins that mediate CALCIUM-dependent CELL ADHESION and are core components of DESMOSOMES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
A glycogen synthase kinase that was originally described as a key enzyme involved in glycogen metabolism. It regulates a diverse array of functions such as CELL DIVISION, microtubule function and APOPTOSIS.
Also known as CD104 antigen, this protein is distinguished from other beta integrins by its relatively long cytoplasmic domain (approximately 1000 amino acids vs. approximately 50). Five alternatively spliced isoforms have been described.
This intrgrin is a key component of HEMIDESMOSOMES and is required for their formation and maintenance in epithelial cells. Integrin alpha6beta4 is also found on thymocytes, fibroblasts, and Schwann cells, where it functions as a laminin receptor (RECEPTORS, LAMININ) and is involved in wound healing, cell migration, and tumor invasiveness.
Integrin beta chains combine with integrin alpha chains to form heterodimeric cell surface receptors. Integrins have traditionally been classified into functional groups based on the identity of one of three beta chains present in the heterodimer. The beta chain is necessary and sufficient for integrin-dependent signaling. Its short cytoplasmic tail contains sequences critical for inside-out signaling.
A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.
Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
An integrin found on fibroblasts, platelets, endothelial and epithelial cells, and lymphocytes where it functions as a receptor for COLLAGEN and LAMININ. Although originally referred to as the collagen receptor, it is one of several receptors for collagen. Ligand binding to integrin alpha2beta1 triggers a cascade of intracellular signaling, including activation of p38 MAP kinase.
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). The adrenergic beta-2 receptors are more sensitive to EPINEPHRINE than to NOREPINEPHRINE and have a high affinity for the agonist TERBUTALINE. They are widespread, with clinically important roles in SKELETAL MUSCLE; LIVER; and vascular, bronchial, gastrointestinal, and genitourinary SMOOTH MUSCLE.
A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A rac GTP-binding protein involved in regulating actin filaments at the plasma membrane. It controls the development of filopodia and lamellipodia in cells and thereby influences cellular motility and adhesion. It is also involved in activation of NADPH OXIDASE. This enzyme was formerly listed as EC 3.6.1.47.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). The adrenergic beta-1 receptors are equally sensitive to EPINEPHRINE and NOREPINEPHRINE and bind the agonist DOBUTAMINE and the antagonist METOPROLOL with high affinity. They are found in the HEART, juxtaglomerular cells, and in the central and peripheral nervous systems.
A group of desmosomal cadherins with cytoplasmic tails that resemble those of classical CADHERINS.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN. It is widely expressed during development, but in the adult is the predominant laminin receptor (RECEPTORS, LAMININ) in mature SMOOTH MUSCLE CELLS, where it is important for maintenance of the differentiated phenotype of these cells. Integrin alpha1beta1 is also found in LYMPHOCYTES and microvascular endothelial cells, and may play a role in angiogenesis. In SCHWANN CELLS and neural crest cells, it is involved in cell migration. Integrin alpha1beta1 is also known as VLA-1 and CD49a-CD29.
The barrier between capillary blood and alveolar air comprising the alveolar EPITHELIUM and capillary ENDOTHELIUM with their adherent BASEMENT MEMBRANE and EPITHELIAL CELL cytoplasm. PULMONARY GAS EXCHANGE occurs across this membrane.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.
A scaffolding protein that is a critical component of the axin signaling complex which binds to ADENOMATOUS POLYPOSIS COLI PROTEIN; GLYCOGEN SYNTHASE KINASE 3; and CASEIN KINASE I.
Proteins prepared by recombinant DNA technology.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A cell line derived from cultured tumor cells.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.

Alzheimer's disease: clues from flies and worms. (1/5919)

Presenilin mutations give rise to familial Alzheimer's disease and result in elevated production of amyloid beta peptide. Recent evidence that presenilins act in developmental signalling pathways may be the key to understanding how senile plaques, neurofibrillary tangles and apoptosis are all biochemically linked.  (+info)

The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin. (2/5919)

Ubiquitin-conjugation targets numerous cellular regulators for proteasome-mediated degradation. Thus, the identification of ubiquitin ligases and their physiological substrates is crucially important, especially for those cases in which aberrant levels of regulatory proteins (e.g., beta-catenin, p27) result from a deregulated ubiquitination pathway. In yeast, the proteolysis of several G1 regulators is controlled by ubiquitin ligases (or SCFs) formed by three subunits: Skp1, Cul A (Cdc53), and one of many F-box proteins. Specific F-box proteins (Fbps) recruit different substrates to the SCF. Although many Fbps have been identified in mammals, their specific substrates and the existence of multiple SCFs have not yet been reported. We have found that one human Fbp, beta-Trcp (beta-Transducin repeat containing protein), does indeed form a novel SCF with human Skp1 and Cul1. Consistent with recent reports indicating that Xenopus and Drosophila beta-Trcp homologs act as negative regulators of the Wnt/beta-catenin signaling pathway, we report here that human beta-Trcp interacts with beta-catenin in vivo. Furthermore, beta-catenin is specifically stabilized in vivo by the expression of a dominant negative beta-Trcp. These results indicate that the Cul1/Skp1/beta-Trcp complex forms a ubiquitin ligase that mediates the degradation of beta-catenin.  (+info)

Axin prevents Wnt-3a-induced accumulation of beta-catenin. (3/5919)

When Axin, a negative regulator of the Wnt signaling pathway, was expressed in COS cells, it coeluted with glycogen synthase kinase-3beta (GSK-3beta), beta-catenin, and adenomatous polyposis coli protein (APC) in a high molecular weight fraction on gel filtration column chromatography. In this fraction, GSK-3beta, beta-catenin, and APC were co-precipitated with Axin. Although beta-catenin was detected in the high molecular weight fraction in L cells on gel filtration column chromatography, addition of conditioned medium expressing Wnt-3a to the cells increased beta-catenin in the low molecular weight fraction. However, Wnt-3a-dependent accumulation of beta-catenin was greatly inhibited in L cells stably expressing Axin. Axin also suppressed Wnt-3a-dependent activation of Tcf-4 which binds to beta-catenin and acts as a transcription factor. These results suggest that Axin forms a complex with GSK-3beta, beta-catenin, and APC, resulting in the stimulation of the degradation of beta-catenin and that Wnt-3a induces the dissociation of beta-catenin from the Axin complex and accumulates beta-catenin.  (+info)

Glucocorticoid down-regulation of fascin protein expression is required for the steroid-induced formation of tight junctions and cell-cell interactions in rat mammary epithelial tumor cells. (4/5919)

Glucocorticoid hormones, which are physiological regulators of mammary epithelium development, induce the formation of tight junctions in rat Con8 mammary epithelial tumor cells. We have discovered that, as part of this process, the synthetic glucocorticoid dexamethasone strongly and reversibly down-regulated the expression of fascin, an actin-bundling protein that also interacts with the adherens junction component beta-catenin. Ectopic constitutive expression of full-length mouse fascin containing a Myc epitope tag (Myc-fascin) in Con8 cells inhibited the dexamethasone stimulation of transepithelial electrical resistance, disrupted the induced localization of the tight junction protein occludin and the adherens junction protein beta-catenin to the cell periphery, and prevented the rearrangement of the actin cytoskeleton. Ectopic expression of either the carboxyl-terminal 213 amino acids of fascin, which includes the actin and beta-catenin-binding sites, or the amino-terminal 313 amino acids of fascin failed to disrupt the glucocorticoid induction of tight junction formation. Mammary tumor cells expressing the full-length Myc-fascin remained generally glucocorticoid responsive and displayed no changes in the levels or protein-protein interactions of junctional proteins or the amount of cytoskeletal associated actin filaments. However, a cell aggregation assay demonstrated that the expression of Myc-fascin abrogated the dexamethasone induction of cell-cell adhesion. Our results implicate the down-regulation of fascin as a key intermediate step that directly links glucocorticoid receptor signaling to the coordinate control of junctional complex formation and cell-cell interactions in mammary tumor epithelial cells.  (+info)

Frequent nuclear/cytoplasmic localization of beta-catenin without exon 3 mutations in malignant melanoma. (5/5919)

Beta-Catenin has a critical role in E-cadherin-mediated cell-cell adhesion, and it also functions as a downstream signaling molecule in the wnt pathway. Mutations in the putative glycogen synthase kinase 3beta phosphorylation sites near the beta-catenin amino terminus have been found in some cancers and cancer cell lines. The mutations render beta-catenin resistant to regulation by a complex containing the glycogen synthase kinase 3beta, adenomatous polyposis coli, and axin proteins. As a result, beta-catenin accumulates in the cytosol and nucleus and activates T-cell factor/ lymphoid enhancing factor transcription factors. Previously, 6 of 27 melanoma cell lines were found to have beta-catenin exon 3 mutations affecting the N-terminal phosphorylation sites (Rubinfeld B, Robbins P, Elgamil M, Albert I, Porfiri E, Polakis P: Stabilization of beta-catenin by genetic defects in melanoma cell lines. Science 1997, 275:1790-1792). To assess the role of beta-catenin defects in primary melanomas, we undertook immunohistochemical and DNA sequencing studies in 65 melanoma specimens. Nuclear and/or cytoplasmic localization of beta-catenin, a potential indicator of wnt pathway activation, was seen focally within roughly one third of the tumors, though a clonal somatic mutation in beta-catenin was found in only one case (codon 45 Ser-->Pro). Our findings demonstrate that beta-catenin mutations are rare in primary melanoma, in contrast to the situation in melanoma cell lines. Nonetheless, activation of beta-catenin, as indicated by its nuclear and/or cytoplasmic localization, appears to be frequent in melanoma, and in some cases, it may reflect focal and transient activation of the wnt pathway within the tumor.  (+info)

Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway. (6/5919)

Overexpression of cell surface glycoproteins of the CD44 family is an early event in the colorectal adenoma-carcinoma sequence. This suggests a link with disruption of APC tumor suppressor protein-mediated regulation of beta-catenin/Tcf-4 signaling, which is crucial in initiating tumorigenesis. To explore this hypothesis, we analyzed CD44 expression in the intestinal mucosa of mice and humans with genetic defects in either APC or Tcf-4, leading to constitutive activation or blockade of the beta-catenin/Tcf-4 pathway, respectively. We show that CD44 expression in the non-neoplastic intestinal mucosa of Apc mutant mice is confined to the crypt epithelium but that CD44 is strongly overexpressed in adenomas as well as in invasive carcinomas. This overexpression includes the standard part of the CD44 (CD44s) as well as variant exons (CD44v). Interestingly, deregulated CD44 expression is already present in aberrant crypt foci with dysplasia (ACFs), the earliest detectable lesions of colorectal neoplasia. Like ACFs of Apc-mutant mice, ACFs of familial adenomatous polyposis (FAP) patients also overexpress CD44. In sharp contrast, Tcf-4 mutant mice show a complete absence of CD44 in the epithelium of the small intestine. This loss of CD44 concurs with loss of stem cell characteristics, shared with adenoma cells. Our results indicate that CD44 expression is part of a genetic program controlled by the beta-catenin/Tcf-4 signaling pathway and suggest a role for CD44 in the generation and turnover of epithelial cells.  (+info)

Cadherin-11 is expressed in invasive breast cancer cell lines. (7/5919)

In several cancers, including breast cancer, loss of E-cadherin expression is correlated with a loss of the epithelial phenotype and with a gain of invasiveness. Cells that have lost E-cadherin expression are either poorly invasive with a rounded phenotype, or highly invasive, with a mesenchymal phenotype. Most cells lacking E-cadherin still retain weak calcium-dependent adhesion, indicating the presence of another cadherin family member. We have now examined the expression of the mesenchymal cadherin, cadherin-11, in breast cancer cell lines. Cadherin-11 mRNA and protein, as well as a variant form, are expressed in the most invasive cell lines but not in any of the noninvasive cell lines. Cadherin-11 is localized to a detergent-soluble pool and is associated with both alpha- and beta-catenin. Immunocytochemistry shows that cadherin-11 is localized to the cell membrane at sites of cell-cell contact as well as at lamellipodia-like projections, which do not interact with other cells. These results suggest that cadherin-11 expression may be well correlated with the invasive phenotype in cancer cells and may serve as a molecular marker for the more aggressive, invasive subset of tumors. Cadherin-11 may mediate the interaction between malignant tumor cells and other cell types that normally express cadherin-11, such as stromal cells or osteoblasts or perhaps even with the surrounding extracellular matrix, thus facilitating tumor cell invasion and metastasis.  (+info)

Coupling assembly of the E-cadherin/beta-catenin complex to efficient endoplasmic reticulum exit and basal-lateral membrane targeting of E-cadherin in polarized MDCK cells. (8/5919)

The E-cadherin/catenin complex regulates Ca++-dependent cell-cell adhesion and is localized to the basal-lateral membrane of polarized epithelial cells. Little is known about mechanisms of complex assembly or intracellular trafficking, or how these processes might ultimately regulate adhesion functions of the complex at the cell surface. The cytoplasmic domain of E-cadherin contains two putative basal-lateral sorting motifs, which are homologous to sorting signals in the low density lipoprotein receptor, but an alanine scan across tyrosine residues in these motifs did not affect the fidelity of newly synthesized E-cadherin delivery to the basal-lateral membrane of MDCK cells. Nevertheless, sorting signals are located in the cytoplasmic domain since a chimeric protein (GP2CAD1), comprising the extracellular domain of GP2 (an apical membrane protein) and the transmembrane and cytoplasmic domains of E-cadherin, was efficiently and specifically delivered to the basal-lateral membrane. Systematic deletion and recombination of specific regions of the cytoplasmic domain of GP2CAD1 resulted in delivery of <10% of these newly synthesized proteins to both apical and basal-lateral membrane domains. Significantly, >90% of each mutant protein was retained in the ER. None of these mutants formed a strong interaction with beta-catenin, which normally occurs shortly after E-cadherin synthesis. In addition, a simple deletion mutation of E-cadherin that lacks beta-catenin binding is also localized intracellularly. Thus, beta-catenin binding to the whole cytoplasmic domain of E-cadherin correlates with efficient and targeted delivery of E-cadherin to the lateral plasma membrane. In this capacity, we suggest that beta-catenin acts as a chauffeur, to facilitate transport of E-cadherin out of the ER and the plasma membrane.  (+info)

Aberrant activation of Wnt/beta-catenin signaling is recognized as a critical factor in the etiology of colorectal cancer. Evidence has suggested that dysregulated beta-catenin activity is associated with the majority of colon cancers via activation of the expression of Wnt regulated oncogenes. In the nucleus, beta-catenin regulates transcription by recruiting additional coactivators. These coactivators all have distinct and unique functions on Wnt/beta-catenin target gene activation. Here we report two coactivators for beta-catenin-mediated transcription: CCAR1 (Cell Cycle and Apoptosis Regulator 1) and CARM1 (coactivator-associated-protein-arginine-methyltransferase 1). We show that both CCAR1 and CARM1 interact with beta-catenin and positively modulate beta-catenin-mediated gene expression. In colorectal cancer cells, which have constitutively high Wnt/beta-catenin activity, depletion of CCAR1 or CARM1 inhibits the expression of Wnt/beta-catenin-mediated oncogenes and suppresses ...
Aberrant beta-catenin expression as determined by assessment of its subcellular localization constitutes a surrogate marker of Wnt signalling pathway activation and has been reported in a subset of breast cancers. The association of beta-catenin/Wnt pathway activation with clinical outcome and the mechanisms leading to its activation in breast cancers still remain a matter of controversy. The aims of this study were to address the distribution of beta-catenin expression in invasive breast cancers, the correlations between beta-catenin expression and clinicopathological features and survival of breast cancer patients, and to determine whether aberrant beta-catenin expression is driven by CTNNB1 (beta-catenin encoding gene) activating mutations. Immunohistochemistry was performed on a tissue microarray containing 245 invasive breast carcinomas from uniformly treated patients, using two anti-beta-catenin monoclonal antibodies. Selected samples were subjected to CTNNB1 exon 3 mutation analysis by ...
SMAD4 has been suggested to inhibit the activity of WNT/beta-catenin signaling pathway in cancer. However, the mechanism by which SMAD4 antagonizes WNT/beta-catenin signaling in cancer remains largely unknown. Aurora A kinase (AURKA), which is frequently overexpressed in cancer, increases the transcriptional activity of beta-catenin/T cell factor (TCF) complex by stabilizing beta-catenin through the inhibition of GSK-3beta. Here, SMAD4 modulated AURKA in a TGF-beta-independent manner. Overexpression of SMAD4 significantly suppressed AURKA function including colony formation, migration, and invasion of cell lines. In addition, SMAD4 bound to AURKA, induced degradation of AURKA by the proteasome. A luciferase activity assay revealed that the transcriptional activity of the beta-catenin/TCF complex was elevated by AURKA, but decreased by SMAD4 overexpression. Moreover, target gene analysis showed that SMAD4 abrogated the AURKA-mediated increase of beta-catenin target genes. However, this inhibitory ...
In the canonical Wnt signaling pathway, beta-catenin activates target genes through its interactions with Tcf/Lef-family transcription factors and additional transcriptional coactivators. The crystal structure of ICAT, an inhibitor of beta-catenin-mediated transcription, bound to the armadillo repeat domain of beta-catenin, has been determined. ICAT contains an N-terminal helilical domain that binds to repeats 11 and 12 of beta-catenin, and an extended C-terminal region that binds to repeats 5-10 in a manner similar to that of Tcfs and other beta-catenin ligands. Full-length ICAT dissociates complexes of beta-catenin, Lef-1, and the transcriptional coactivator p300, whereas the helical domain alone selectively blocks binding to p300. The C-terminal armadillo repeats of beta-catenin may be an attractive target for compounds designed to disrupt aberrant beta-catenin-mediated transcription associated with various cancers. ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors ...
beta Catenin, clone: 15B8, eBioscience™ 100μg; Unconjugated beta Catenin, clone: 15B8, eBioscience™ Primary Antibodies Cas to Caz
The Wnt/Beta-catenin pathway mediates the transcription of proteins important for maintenance and growth of hematopoietic stem cells. The inhibition of Wnt leads to protein degradation through Beta-Catenin activation by the Axin/APC/CK1/GSK3B protein complex. This pathway is based on figure 5 from Misaghian et al ...
By means of the fluorescent differential display method, we isolated novel mouse and human genes, Drctnnb1a and DRCTNNB1A, the expression levels of which were inversely correlated to the amount of β-catenin present in cells. Recent reports have identified a number of mammalian genes including c-myc (6) , cyclin D1 (7) , matrilysin (8) , WISP (9) , c-jun, fra-1, uPAR, ZO-1 (10) , and NBL4 (11) that are regulated by stabilization and activation of β-catenin. In Xenopus or Drosophila, target genes for Wnt signaling include the nodal-related 3 gene, Xnr3 (17) , a member of the transforming growth factor-β superfamily, and homeobox genes engrailed (18) , goosecoid, siamois (17) , twin (19) , ultrabithorax (20) , and fibronectin (21) . Among those reported molecules, all but ZO-1 appeared to be up-regulated byβ -catenin through transactivation of Tcf/Lef transcription factors. Hence, DRCTNNB1A is only the second gene to be identified as down-regulated by the accumulation of β-catenin. ...
The PNU-74654 (PNU) compound is a non-FDAapproved drug which prevents that Tcf from binding to beta-catenin, acting as a Wnt/beta-catenin antagonist. In NCI-H295 cells,PNU-74654 significantly decreases cell proliferation 96 h after treatment, increases early and late apoptosis, decreases nuclear betacatenin accumulation, impairs CTNNB1/beta-catenin expression and increases betacatenin target genes 48 h after treatment. No effects are observed on HeLa cells. In NCI-H295 cells, PNU-74654 decreases cortisol, testosterone and androstenedione secretion 24 and 48 h after treatment. Additionally, in NCI-H295 cells, PNU-74654 decreases SF1 and CYP21A2 mRNA expression as well as the protein levels of STAR and aldosterone synthase 48 h after treatment. In Y1 cells, PNU-74654 impairs corticosterone secretion 24 h after treatment but does not decrease cell viability[2]. ...
Billin et al. (23) observed that TSA activated TOPflash reporter activity in HEK293 cells in the presence and absence of exogenous β-catenin and LEF1, and provided evidence that HDAC1 switches LEF1 from a repressor to a transcriptional activator. Our results confirm that TSA increases TOPflash reporter activity, and demonstrate that the effect of HDAC1 on LEF1 can be extended to TCF4, the major form of TCF/LEF in colonic mucosa. Thus, under certain circumstances, HDAC1 might influence whether TCF4 acts as a transcriptional activator or repressor in the Wnt signaling pathway. The present results also show that TOPflash reporter activity can be used as an indirect measure of HDAC activity, with an increase in reporter activity corresponding to a decrease in HDAC activity in cells treated with TSA or SFN.. SFN is an effective cancer chemopreventive agent in several animal models (10, 11, 12) and is thought to induce phase 2 detoxification enzymes through the interaction of Nrf-2 with the ...
Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases (PubMed:25753423). May also function in Wnt signaling by promoting the rapid degradation of CTNNB1 (PubMed:10021369, PubMed:11691822, PubMed:9823329). This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The developmental signals that govern cell specification and differentiation in vertebrate somites are well understood. However, little is known about the downstream signalling pathways involved. We have shown previously that a combination of Shh protein and Wnt1 or Wnt3a-expressing fibroblasts is sufficient to activate skeletal muscle-specific gene expression in somite explants. Here, we have examined the molecular mechanisms by which the Wnt-mediated signal acts on myogenic precursor cells. We show that chick frizzled 1 (Fz1), beta-catenin and Lef1 are expressed during somitogenesis. Lef1 and beta-catenin transcripts become restricted to the developing myotome. Furthermore, beta-catenin is expressed prior to the time at which MyoD transcripts can be detected. Expression of beta-catenin mRNA is regulated by positive and negative signals derived from neural tube, notochord and lateral plate mesoderm. These signals include Bmp4, Shh and Wnt1/Wnt3a itself. In somite explants, Fz1, beta-catenin and ...
Kim B، Koo H، Yang S، Bang S، Jung Y، Kim Y، Kim J، Park J، Moon RT، Song K، Lee I (June 2006). TC1(C8orf4) correlates with Wnt/beta-catenin target genes and aggressive biological behavior in gastric cancer. Clinical Cancer Research. 12 (11 Pt 1): 3541-8. PMID 16740781. doi:10.1158/1078-0432.CCR-05-2440. ...
Sigma-Aldrich offers abstracts and full-text articles by [Laurent Pangon, Dessislava Mladenova, Lauren Watkins, Christa Van Kralingen, Nicola Currey, Sam Al-Sohaily, Patrick Lecine, Jean-Paul Borg, Maija R J Kohonen-Corish].
Six homeoproteins. We have shown that the SIX homeoproteins family was expressed throughout muscle development in mammals, controlling bHLH MRF4 myogenin and MyoD determination genes in the embryo, as well as the aldolase A gene, which is specifically expressed in adult fast IIB fibers. We aim to determine the functions of SIX proteins and their EYA cofactors in the control of muscle morphogenesis in the mouse, their involvement in muscle myofiber specification, and to define the genetic targets of the SIX-EYA complex. To allow this study we generated a Six1 as well as a double Six1-Six4 invalidation models, and obtained Eya1 and Eya2 deficient animals from P-X. Xu (USA) Six2 deficient mice from G.Oliver (USA) and Six5 deficient mice from S. Tapscott (USA). These different mouse models have allowed us to begin to characterize the functions of SIX and EYA proteins during mouse embryogenesis. The second aspect of our work, is to characterize the contribution to muscle diversity of the Six-Eya ...
Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted ...
14-3-3ζ has been found to associate with β-catenin (Tian et al., 2004). Later, it was found that Akt phosphorylates β-catenin at serine 552, which appears to enhance its interaction with 14-3-3ζ (Fang et al., 2007). In both cases, ectopic expression of 14-3-3ζ resulted in a moderate activation (two- to fourfold) of β-catenin-dependent transcription in TopFlash assays. We found that 14-3-3ζ enhances, whereas 14-3-3η and ε isoforms repress, β-catenin activation of the TopFlash reporter (Fig. 5 A). One possible explanation for this observation is that 14-3-3 overexpression exerts complex biological effects, which makes our interpretation of the TopFlash results difficult. In fact, 14-3-3 proteins have been shown to interact with a plethora of target proteins ranging from transcription factors to various signaling molecules (Dougherty and Morrison, 2004; Pozuelo Rubio et al., 2004). However, it is interesting to note that, consistent with our results (Fig. 7 C), ectopic expression of ...
In Xenopus, Wnt signals and their transcriptional effector beta-catenin are required for the development of dorsal axial structures. In zebrafish, previous loss-of-function studies have not identified an essential role for beta-catenin in dorsal axis
Chandran K C ♦ December 11, 2015 ♦ Leave a comment Scientists and cancer researchers have lately identified a particular biological molecule in our body known as BETA CATENIN as an ideal molecular target for anti-cancer therapy. They have been trying to develop drugs that could inhibit the over-expressions and aberrations of BETA CATENIN, which is recognized to be playing a big role in the biochemical processes underlying various types of cancers. Their attempts have not been so far successful, since any chemical compound they develop to target BETA CATENIN will inevitably have serious harmful effects upon the organism, since it is an essential biological molecule having diverse roles normal vital processes, and its complete inhibition may lead to be very dangerous consequences.. BETA CATENIN is a protein found as part of molecular complexes in forming cadherin cell adhesion factors of animal cells. It belongs to a family of biological compounds known as catenins, consisting of alpha ...
View Notes - 2011_Questions_Week_14_Answers from BIO 349 at University of Texas. 1. What happens when you deplete beta catenin in planarians? -The organism is no longer able to form a posterior side
Background: β‐Catenin is an important signaling molecule in the Wnt pathway that plays a key role in tumorgenesis. In the absence of Wnt signaling, the cytoplasmic level of β‐catenin is kept low due to rapid proteasomal‐mediated degradation of GSK3β phosphorylated β‐catenin. Activation of Wnt signaling leads to the inactivation of GSK3β, resulting in stabilization and accumulation of β‐catenin in the cytoplasm. Consequently, β‐catenin translocates into the nucleus, where it binds with members of the T‐cell factor (Tcf)/lymphocyte enhancer‐binding factor family of transcription factors and activates the expression of many target genes important for cancer development. Most colon cancers have activating mutations in the APC tumor suppressor or in β‐catenin itself. Furthermore, activating β‐catenin mutations have been found in a variety of other tumors such as melanomas, hepatocellular carcinomas, skin, breast, and prostate cancer, whereas β‐catenin is not activated ...
The human oncogene beta-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/beta-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, …
J:215487 Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JL, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A,Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR, A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain. Neuron. 2014 Jul 16;83(2):309-23 ...
J:90567 Akiyama H, Lyons JP, Mori-Akiyama Y, Yang X, Zhang R, Zhang Z, Deng JM, Taketo MM, Nakamura T, Behringer RR, McCrea PD, de Crombrugghe B, Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev. 2004 May 1;18(9):1072-87 ...
Rabbit polyclonal beta Catenin (phospho Y489) antibody validated for WB, ICC and tested in Human. Immunogen corresponding to synthetic peptide
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Zhang, J., G. J. Woodhead, S. K. Swaminathan, S. R. Noles, E. R. McQuinn, A. J. Pisarek, A. M. Stocker, C. A. Mutch, N. Funatsu, and A. Chenn, Cortical neural precursors inhibit their own differentiation via N-cadherin maintenance of beta-catenin signaling., Dev Cell, vol. 18, issue 3, pp. 472-9, 2010 Mar 16. PMCID: PMC2865854 PMID: 20230753 ...
Looking for online definition of Beta catenin in the Medical Dictionary? Beta catenin explanation free. What is Beta catenin? Meaning of Beta catenin medical term. What does Beta catenin mean?
Lymphoid enhancer-binding factor 1 (LEF1) is a protein that in humans is encoded by the LEF1 gene. Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). LEF1 is highly overexpressed and associated with disease progression and poor prognosis in B-cell chronic lymphocytic leukemia. It is also a promising potential drug target. Lymphoid enhancer-binding factor 1 has been shown to interact with: ALX4, AML-1, CTNNB1, EP300, MITF PIAS4, SMAD2, and SMAD3. GRCh38: Ensembl release 89: ENSG00000138795 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000027985 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Milatovich A, Travis A, Grosschedl R, Francke U (Mar 1992). Gene for lymphoid ...
US Biological Anti-Catenin, beta (Beta Catenin, Cadherin-associated Protein, Catenin beta 1, Catenin beta-1, CATNB, CTNNB, CTNNB1) SKU: C2069-51C()
We show that expression of stabilized β-catenin decreased neurite initiation and elongation in NGF-treated PC12 cells (Fig. 5). Several mechanisms could explain how stabilized β-catenin inhibits neurite outgrowth in PC12 cells. When β-catenin is stabilized by Wnt signals it can promote cadherin-mediated cell-cell adhesion (Hinck et al., 1994) in addition to Tcf/Lef-mediated transcription. Experiments expressing stabilized β-catenin in whole animals or in neuronal cultures directly contacting glial cells may mask the role of β-catenin in the APC complex with its role in adhesion (Yu and Malenka, 2004; Loureiro et al., 2001; Elul et al., 2003). Previous work on the role of β-catenin in branching of axons and dendrites uses neurons in direct cell-cell contact with a glial feeder layer, and β-catenin is thought to require N-cadherin for this effect (Yu and Malenka, 2003; Yu and Malenka, 2004). PC12 cells do not form distinct axons and dendrites (Greene et al., 1998) and, if treated with NGF ...
The Wnt signal transduction pathway is important in a wide variety of developmental processes as well as in the genesis of human cancer. Vertebrate Wnt pathways can be functionally separated into two classes, the canonical Wnt/beta-catenin pathway and the non-canonical Wnt/Ca2+ pathway. Supporting differences in Wnt signaling, gain of function of Wnt-1 in C57mg mouse mammary epithelial cells leads to their morphological transformation while loss of function of Wnt-5a leads to the same transformation. Many downstream target genes of the Wnt/beta-catenin pathway have been identified. In contrast, little is known about the Wnt/Ca2+ pathway and whether it regulates gene expression. To test the hypothesis that a specific cell line can respond to distinct Wnts with different patterns of gene expression, we over-expressed Wnt-5a and Rfz-2 in C57mg mammary epithelial cells and compared this cell line to C57mg cells over-expressing Wnt-1. These Wnts were chosen since previous studies suggest that C57mg cells
Akt-regulated pathways enhance cell division and cell survival. Metabolic regulation through Akt and its targets is important for insulin and insulin-like growth factor I-coupled responses. Inhibition of glycogen synthase kinase-3 by Akt-dependent phosphorylation promotes accumulation of β-catenin, which forms complexes with T-cell factor/lymphoid enhancer factor transcription factors and transcriptionally up-regulates cyclin D1, Myc, and other positive growth regulators. Cyclin D1 is also inhibited by glycogen synthase kinase-3 through effects on stability and localization (7) . Concomitantly, Akt phosphorylation of cyclin-dependent protein kinase inhibitors p21Cip1 and p27Kip1 interferes with negative growth regulation (3 , 8) .. Phosphorylation of Mdm2 by Akt enhances nuclear entry, which promotes ubiquitin-dependent proteolysis of p53, and impedes p53-dependent growth suppression and apoptosis (9) . Akt directly forestalls apoptosis by phosphorylation of proapoptotic Bad and caspase-9, ...
Catenin Beta 1, CTNNB are cell adhesion molecules called (p120*␠-catenin) cadherins (the (CDH1) E-cadherin/catenin complex) include the beta-catenins a multifunctional molecule Locus: 3p22.1 [§§; ^]. Neurons also exhibited a higher CTNNB/TCF pathway association (concentration versus accumulation) with cadherins; CAS-chromosome segregation 1-like (yeast) binds with E-cadherin but not with beta-catenin. Which interacts with (Tcf-T-cell factor where a…
A key component of the Wnt pathway, beta-catenin combines with alpha-catenin and regulates cell-cell adhesion. It also interacts with alpha-catenin in the nucleus.. The alpha-catenin component of this beta-catenin/alpha-catenin complex has an inhibitory effect on beta-catenin that helps keep tumor cell migration and invasion in check. This inhibition is lost, however, when the EGFR pathway is activated. Upon activation, beta-catenin becomes untethered from alpha-catenin and translocates to the cell nucleus, where it increases expression of key target genes involved in tumor cell invasion and metastasis.. New Pathway Regulates Beta-Catenin Transactivation. The M. D. Anderson-led team made a surprising discovery: Beta-catenin also can travel to the nucleus via activation of the EGFR pathway-and it does so independently of Wnt signaling or mutations. The newly described pathway disrupts the beta-catenin/alpha catenin complex through an EGFR-extracellular receptor kinase (ERK)-protein kinase CK2- ...
BCL9 and PYGO are beta-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signaling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing mouse forelimbs, BCL9 proteins sustain the action of beta-catenin in a largely PYGO-independent manner. Our genetic analyses implied that BCL9 necessitates other interaction partners in mediating its transcriptional output. We identified the transcription factor TBX3 as a candidate tissue-specific member of the beta-catenin transcriptional complex. In developing forelimbs, both TBX3 and BCL9 occupy a large number of Wnt-responsive regulatory elements, genome-wide. Moreover, mutations in Bcl9 affect the expression of TBX3 targets in vivo, and modulation of TBX3 abundance impacts on Wnt target genes transcription in a beta-catenin- and TCF/LEF-dependent manner. Finally, TBX3 overexpression exacerbates the metastatic potential of Wnt-dependent
beta-catenin destruction complex, beta-catenin-TCF7L2 complex, catenin complex, cell cortex, cell junction, cell periphery, cell-cell adherens junction, cell-cell junction, centrosome, cytoplasm
The canonical Wnt/beta-catenin pathway plays a key role in the regulation of bone remodeling in mice and humans. Two transmembrane proteins that are involved in decreasing the activity of this pathway by binding to extracellular antagonists, such as Dickkopf 1 (Dkk1), are the low-density lipoprotein receptor related protein 5 (Lrp5) and Kremen 2 (Krm2). Lrp 5 deficiency (Lrp5(-/-)) as well as osteoblast-specific overexpression of Krm2 in mice (Col1a1-Krm2) result in severe osteoporosis occurring at young age. In this study, we analyzed the influence of Lrp5 deficiency and osteoblast-specific overexpression of Krm2 on fracture healing in mice using flexible and semi-rigid fracture fixation. We demonstrated that fracture healing was highly impaired in both mouse genotypes, but that impairment was more severe in Col1a1-Krm2 than in Lrp5(-/-) mice and particularly evident in mice in which the more flexible fixation was used. Bone formation was more reduced in Col1a1-Krm2 than in Lrp5(-/-) mice, whereas
3.0.CO;2-P. PMID 10580987. McCrea PD, Turck CW, Gumbiner B (November 1991). A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin. Science. 254 (5036): 1359-61. doi:10.1126/science.1962194. PMID 1962194. Kemler R (September 1993). From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion. Trends in Genetics. 9 (9): 317-21. doi:10.1016/0168-9525(93)90250-l. PMID 8236461. Gottardi CJ, Peifer M (March 2008). Terminal regions of beta-catenin come into view. Structure. 16 (3): 336-8. doi:10.1016/j.str.2008.02.005. PMC 2329800 . PMID 18334207. Xing Y, Takemaru K, Liu J, Berndt JD, Zheng JJ, Moon RT, Xu W (March 2008). Crystal structure of a full-length beta-catenin. Structure. 16 (3): 478-87. doi:10.1016/j.str.2007.12.021. PMC 4267759 . PMID 18334222. Vleminckx K, Kemler R, Hecht A (March 1999). The C-terminal transactivation domain of beta-catenin is necessary and sufficient for signaling by the LEF-1/beta-catenin complex ...
The transcriptional coactivator beta-catenin mediates Wnt growth factor signaling. In the absence of a Wnt signal, casein kinase 1 (CK1) and glycogen synthase kinase-3beta (GSK-3beta) phosphorylate cytosolic beta-catenin, thereby flagging it for recognition and destruction by the ubiquitin/proteosom …
The canonical Wnt/beta-catenin pathway plays a key role in the regulation of bone remodeling in mice and humans. Two transmembrane proteins that are involved in decreasing the activity of this pathway by binding to extracellular antagonists, such as Dickkopf 1 (Dkk1), are the low-density lipoprotein receptor related protein 5 (Lrp5) and Kremen 2 (Krm2). Lrp 5 deficiency (Lrp5(-/-)) as well as osteoblast-specific overexpression of Krm2 in mice (Col1a1-Krm2) result in severe osteoporosis occurring at young age. In this study, we analyzed the influence of Lrp5 deficiency and osteoblast-specific overexpression of Krm2 on fracture healing in mice using flexible and semi-rigid fracture fixation. We demonstrated that fracture healing was highly impaired in both mouse genotypes, but that impairment was more severe in Col1a1-Krm2 than in Lrp5(-/-) mice and particularly evident in mice in which the more flexible fixation was used. Bone formation was more reduced in Col1a1-Krm2 than in Lrp5(-/-) mice, ...
Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
Cadherins play an important role in morphogenesis and have recently been implicated in the regulation of cell proliferation, however the mechanisms by which they function are poorly understood. In the vertebrate CNS, loss of ,italic,N-cadherin (N-cad),,/, results in impaired neuroepithelial integrity. Zebrafish ,italic,N-cad,,/, null mutants also exhibit a transient increase in neurons and in cell proliferation in the neural tube. Here, we investigate the cellular and molecular basis for this phenotype, using multiple ,italic,N-cad,,/, alleles with distinct molecular properties. We confirm that cell proliferation is enhanced in ,italic,N-cad,,/, mutants, but contrary to previous findings, we observe that the increase is sustained over multiple stages of development. At the cellular level, loss of ,italic,N-cad,,/, results in a shorter cell cycle. Furthermore, we demonstrate that hyperproliferation is not linked to abnormal beta-catenin localization, suggesting that Wnt signaling is not ...
Cadherins play an important role in morphogenesis and have recently been implicated in the regulation of cell proliferation, however the mechanisms by which they function are poorly understood. In the vertebrate CNS, loss of ,italic,N-cadherin (N-cad),,/, results in impaired neuroepithelial integrity. Zebrafish ,italic,N-cad,,/, null mutants also exhibit a transient increase in neurons and in cell proliferation in the neural tube. Here, we investigate the cellular and molecular basis for this phenotype, using multiple ,italic,N-cad,,/, alleles with distinct molecular properties. We confirm that cell proliferation is enhanced in ,italic,N-cad,,/, mutants, but contrary to previous findings, we observe that the increase is sustained over multiple stages of development. At the cellular level, loss of ,italic,N-cad,,/, results in a shorter cell cycle. Furthermore, we demonstrate that hyperproliferation is not linked to abnormal beta-catenin localization, suggesting that Wnt signaling is not ...
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
Calcium-dependent homotypic cell-cell adhesion, mediated by molecules such as E-cadherin, guides the establishment of classical epithelial cell polarity and contributes to the control of migration, growth, and differentiation. These actions involve additional proteins, including alpha- and beta-catenin (or plakoglobin) and p120, as well as linkage to the cortical actin cytoskeleton. The molecular basis for these interactions and their hierarchy of interaction remain controversial. We demonstrate a direct interaction between F-actin and alpha (E)-catenin, an activity not shared by either the cytoplasmic domain of E-cadherin or beta-catenin. Sedimentation assays and direct visualization by transmission electron microscopy reveal that alpha 1(E)-catenin binds and bundles F-actin in vitro with micromolar affinity at a catenin/G-actin monomer ratio of approximately 1:7 (mol/mol). Recombinant human beta-catenin can simultaneously bind to the alpha-catenin/actin complex but does not bind actin ...
The farnesoid X receptor (FXR) controls the synthesis and transport of bile acids (BAs). Mice lacking expression of FXR, designated Fxr-null, have elevated levels of serum and hepatic BAs and an increase in BA pool size. Surprisingly, at 12 months of age, male and female Fxr-null mice had a high incidence of degenerative hepatic lesions, altered cell foci and liver tumors including hepatocellular adenoma, carcinoma and hepatocholangiocellular carcinoma, the latter of which is rarely observed in mice. At 3 months, Fxr-null mice had increased expression of the proinflammatory cytokine IL-1beta mRNA and elevated beta-catenin and its target gene c-myc. They also had increased cell proliferation as revealed by increased PCNA mRNA and BrdU incorporation. These studies reveal a potential role for FXR and BAs in hepatocarcinogenesis.
The gene strabismus (stbm)/Van Gogh (Vang) functions in the planar cell-polarity pathway in Drosophila. As the existence of such a pathway in vertebrates has not been firmly established, we investigated the functions and signalling activities encoded by stbm in vertebrate embryos. In regard to cell fate, inhibition of Stbm function in zebrafish embryos leads to reduction of anterior neural markers, whereas gain of function leads to a rise in the levels of these markers. In regard to cell behaviour, both gain-of-function and loss-of-function assays reveal a role for Stbm in mediating cell movements during gastrulation. Mechanistically, Stbm inhibits Wnt-mediated activation of beta-catenin-dependent transcription while promoting phosphorylation of c-Jun- and AP-1-dependent transcription. This complex effect on intracellular signalling pathways probably involves dishevelled (dsh), as Stbm was found to interact with the Dsh protein, and as Dsh is known to function in both planar cell-polarity and ...
The study, published online in the September 2011 edition of The Journal of Neuroscience, identified Sox17 as the gene that helps regulate the Wnt/beta-catenin signaling pathway during the transition of oligodendrocyte progenitor cells, or immature brain cells, to a more mature, differentiated state where they generate myelin.. This is the first time the Sox17 gene has been identified as a regulator of the Wnt/beta-catenin pathway during myelination, said Li-Jin Chew, PhD, lead author of the study. Our findings indicate that loss of Sox17 over-stimulates the Wnt/beta-catenin pathway and keeps oligodendrocyte progenitor cells from maturing and producing myelin, potentially causing developmental disabilities in developing babies and children.. Myelin is the protective material around the axons of neurons; in mass these types of ensheathed neurons are collectively called white matter. White matter serves as the primary messaging network that conducts signals rapidly between gray matter areas. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex ...
The Wnt/β-catenin signaling pathway plays an important role in renal development and is reexpressed in the injured kidney and other organs. β-Catenin signaling is protective in acute kidney injury (AKI) through actions on the proximal tubule, but the current dogma is that Wnt/β-catenin signaling promotes fibrosis and development of chronic kidney disease (CKD). As the role of proximal tubular β-catenin signaling in CKD remains unclear, we genetically stabilized (i.e., activated) β-catenin specifically in murine proximal tubules. Mice with increased tubular β-catenin signaling were protected in 2 murine models of AKI to CKD progression. Oxidative stress, a common feature of CKD, reduced the conventional T cell factor/lymphoid enhancer factor-dependent β-catenin signaling and augmented FoxO3-dependent activity in proximal tubule cells in vitro and in vivo. The protective effect of proximal tubular β-catenin in renal injury required the presence of FoxO3 in vivo. Furthermore, we identified ...
Background: The Wnt/beta-catenin signaling pathway plays a key role in stem cell maintenance in the colorectum. Rare high penetrance genetic mutations in components of this pathway result in familial colorectal cancer, yet the impact of common, germline variants remains unknown. Methods: We assessed 172 variants in 26 genes from the Wnt/beta-catenin pathway in 809 CRC cases and 814 healthy controls, followed by replication of the top findings in another 691 cases and 775 controls. In silico informatic tools were used to predict functional effects of variants. Results: Eighteen SNPs in the pathway were significantly associated with CRC risk (P ,0.05) in the discovery phase. We observed a significant dose-response increase in CRC risk by number of risk genotypes carried (P = 4.19 x 10-8). Gene-based analysis implicated CSNK1D (P = 0.014), FZD3 (P = 0.023), and APC (P = 0.027) as significant for CRC risk. In the replication phase, FZD3:rs11775139 remained significantly associated with reduced risk ...
(a) Nuclear suprabasal beta-catenin (β-catenin) staining expression in all layers of epidermis except the parakeratotic and cornified layers of a psoriasis les
LEF1 antibody [1C3.1D10] (lymphoid enhancer-binding factor 1) for WB. Anti-LEF1 mAb (GTX12033) is tested in Human samples. 100% Ab-Assurance.
1I7X: The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin.
ORoak and other (2012) sequenced the exomes, that is the DNA regions that code for the protein product of genes, of children with sporadic autism as well as of their parents and unaffected siblings. Sixhundredseventyseven exomes of 209 families were examined. Eighty percent of the discovered gene mutations were of paternal origin, increasing with age. Roughly 40 percent of the new protein-altering mutations were associated with a molecular signaling pathway regulating gene transcription through beta-catenin/chromatin remodeling. Recurrent mutations were found in genes CHD8 and NTNG1. The product of CHD8 is a protein involved in chromatin remodeling. This finding points at a specific molecular mechanism that may explain impaired transcription of the genetic code, representing the most disruptive genetic modification identified in this study according to the authors. NTNG1s product Netrin-G1 is a protein that serves as cue in nerve cell axon guidance and has been associated with schizophrenia. ...
TGF-β is a key profibrotic factor, but targeting TGF-β to prevent fibrosis also abolishes its protective anti-inflammatory effects. Here, we investigated the hypothesis that we can redirect TGF-β signaling by preventing downstream profibrotic interaction of β-catenin with T cell factor (TCF), thereby enhancing the interaction of β-catenin with Foxo, a transcription factor that controls differentiation of TGF-β induced regulatory T cells (iTregs), and thus, enhance anti-inflammatory effects of TGF-β In iTregs derived from EL4 T cells treated with recombinant human TGF-β1 (rhTGF-β1) in vitro, inhibition of β-catenin/TCF transcription with ICG-001 increased Foxp3 expression, interaction of β-catenin and Foxo1, binding of Foxo1 to the Foxp3 promoter, and Foxo transcriptional activity ...
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.. Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436 ...
CTNNB1 - CTNNB1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector shRNA available for purchase from OriGene - Your Gene Company.
Human tongue surface with bacteria. Coloured scanning electron micrograph (SEM) of bacteria (yellow) amongst epithelial cells on the surface of a human tongue. Epithelial cells are flat, scale-like squamous cells that are constantly shed and replaced. The epithelium covers the sensory papillae (small projections, not seen) on the tongue, which are sensitive to several different sensory stimuli, including taste. Bacteria on the tongue surface is normal, but can cause halitosis (bad breath). Magnification: x1700 when printed 10 centimetres wide. - Stock Image C002/6112
Another study shows FAM158A responds to Beta-catenin depletion. Although there are no known beta-catenin binding sites in the ... there is a NeuroD site and NeuroD responds to beta-catenin. The paralog to FAM158A is commonly known as Cox4NB and is located ... "Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression". Blood. 114 (13): 2699-708. ...
The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated ... A novel link to beta-catenin activation". Cell Cycle. 5 (1): 23-26. doi:10.4161/cc.5.1.2305. PMID 16357527. Seitz, C. S.; Van ...
Schaefer KN, Peifer M (February 2019). "Wnt/Beta-Catenin Signaling Regulation and a Role for Biomolecular Condensates". ... Schaefer KN, Peifer M (February 2019). "Wnt/Beta-Catenin Signaling Regulation and a Role for Biomolecular Condensates". ... including cross-beta polymerisation), and/or protein domains that induce head-to-tail oligomeric or polymeric clustering, might ... Robert Corey and Herman Branson correctly proposed the alpha helix and beta sheet as the primary structural motifs in protein ...
Liu C, Li Y, Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X, He X (March 2002). "Control of beta-catenin phosphorylation/ ... In contrast, in several important targets, NF-AT and beta-catenin, CKI does not require n − 3 priming but, instead, ... "A noncanonical sequence phosphorylated by casein kinase 1 in beta-catenin may play a role in casein kinase 1 targeting of ... "Axin-mediated CKI phosphorylation of beta-catenin at Ser 45: a molecular switch for the Wnt pathway". Genes & Development. 16 ( ...
A mouse model with deregulation of beta-catenin levels was created. The conditional stabilizing mutation in the beta-catenin ... there was substantial nuclear localization of beta-catenin as well as increased cytoplasmic beta-catenin. However, in mice fed ... APC was found to associate with catenins. Today we know that the beta-catenin protein (part of the Wnt signaling pathway) is ... Furthermore, when evaluated for ERCC1, beclin-1, and beta-catenin in the stem cell region of crypts, the colonic tissues of ...
Gao Y, Wang HY (2006). "Casein kinase 2 Is activated and essential for Wnt/beta-catenin signaling". The Journal of Biological ...
More recently, Dyson's group has shown that the transcription factor E2F1 is a potent and specific inhibitor of beta-catenin/T- ... "E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8". Nature. 455 (7212): 552-6. doi:10.1038/ ...
Zhu, AJ; Watt, FM (1999). "beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes ... Evans, RD; Perkins, VC; Henry, A; Stephens, PE; Robinson, MK; Watt, FM (2003). "A tumor-associated beta 1 integrin mutation ...
"Evidence for the Nucleo-Apical Shuttling of a Beta-Catenin Like Plasmodium falciparum Armadillo Repeat Containing Protein". ...
Expression levels of miR-27 are positively correlated with beta-catenin, a key protein in Wnt signalling. There is activation ... which is in response to inhibition of the beta-catenin destruction complex. This in turn is brought about by APC inhibition of ...
... as a modulator of beta-catenin/GSK3 signaling". PLoS ONE. 4 (9): e6892. doi:10.1371/journal.pone.0006892. PMC 2731218. PMID ... receptors prevents amyloid beta oligomer-induced synaptic disruption". Journal of Biological Chemistry. 285 (10): 7619-32. doi: ...
The Nieuwkoop center was discovered to be responsible for dorso-ventral polarity establishment through Wnt/GSK/beta-catenin. ...
... beta-catenin (22.9%), TP53 (22.7%), and ECSIT (19.3%). These genes regulate cell growth and survival. Other genes (e.g. JAK3, ... and β-catenin genes or, alternatively, the TBX21, interferon-γ, and NF-κB genes. Individuals whose malignant cells express the ...
... phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin ... enhancing beta-catenin degradation. GRCh38: Ensembl release 89: ENSG00000107341 - Ensembl, May 2017 GRCm38: Ensembl release 89 ... Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP ... substrate recognition and induces its interaction with beta-TrCP, ...
... cellular domains of E-cadherin molecules bind to the actin cytoskeleton via the adaptor proteins alpha-catenin and beta-catenin ... "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ...
Altered expression of beta-catenin, p21, sonic hedgehog signaling (Shh), and E-cadherin have been associated with invasion, ... Wnt/beta-catenin signaling is known to be altered and play a significant role in the development of sebaceous tumors. ...
B-catenin[edit]. Main article: Beta-catenin. β-catenin of the canonical Wnt signalling pathway plays a role in cell fate ... Main article: Transforming growth factor beta. During mandible development, most of it is formed through intramembranous ...
PDGF-BB is the highest-affinity ligand for the PDGFR-beta; PDGFR-beta is a key marker of hepatic stellate cell activation in ... Age related downregulation of the PDGF receptor on islet beta cells has been demonstrated to prevent islet beta cell ... "Role of alpha beta receptor heterodimer formation in beta platelet-derived growth factor (PDGF) receptor activation by PDGF-AB" ... Two types of PDGFRs have been identified: alpha-type and beta-type PDGFRs.[8] The alpha type binds to PDGF-AA, PDGF-BB and PDGF ...
"Rapamycin potentiates transforming growth factor beta-induced growth arrest in nontransformed, oncogene-transformed, and human ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function.[citation needed] Beta-catenin can ... increases beta-catenin-E-cadherin association, and decreases beta-catenin-sensitive transcription". Cancer Research. 61 (4): ... "The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin". ...
RET is an abbreviation for "rearranged during transfection", as the DNA sequence of this gene was originally found to be rearranged within a 3T3 fibroblast cell line following its transfection with DNA taken from human lymphoma cells.[6] The human gene RET is localized to chromosome 10 (10q11.2) and contains 21 exons.[7] The natural alternative splicing of the RET gene results in the production of 3 different isoforms of the protein RET. RET51, RET43 and RET9 contain 51, 43 and 9 amino acids in their C-terminal tail respectively.[8] The biological roles of isoforms RET51 and RET9 are the most well studied in-vivo as these are the most common isoforms in which RET occurs. Common to each isoform is a domain structure. Each protein is divided into three domains: an N-terminal extracellular domain with four cadherin-like repeats and a cysteine-rich region, a hydrophobic transmembrane domain and a cytoplasmic tyrosine kinase domain, which is split by an insertion of 27 amino acids. Within the ...
Wang P, Gao H, Ni Y, Wang B, Wu Y, Ji L, Qin L, Ma L, Pei G (February 2003). "Beta-arrestin 2 functions as a G-protein-coupled ... Wang P, Wu Y, Ge X, Ma L, Pei G (March 2003). "Subcellular localization of beta-arrestins is determined by their intact N ... In addition, MDM2 has p53-independent transcription factor-like effects in nuclear factor-kappa beta (NFκB) activation. ...
Tryptophan alpha,beta-oxidase. *Pyrroloquinoline-quinone synthase. *L-galactonolactone oxidase. 1.3.5: Quinone. *Succinate ...
beta-catenin-TCF complex assembly. • transcription, DNA-templated. • transcription from RNA polymerase II promoter. • G1/S ... Feng XH, Liang YY, Liang M, Zhai W, Lin X (January 2002). "Direct interaction of c-Myc with Smad2 and Smad3 to inhibit TGF-beta ... "Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor". Journal of Cell Science. 114 (Pt 8): 1533-44. ...
CTNNBL1: Beta-catenin-like protein 1. *DBNDD2: Dysbindin domain-containing protein 2 ...
"Beta-catenin--a linchpin in colorectal carcinogenesis?". The American Journal of Pathology. 160 (2): 389-401. doi:10.1016/s0002 ... regulates the stability of β-catenin in cytoplasm and subsequently, cytosolic β-catenin moves into the nucleus to facilitate ... Transgenic expression of a CD97 in mice enhanced levels of nonphosphorylated membrane-bound β-catenin and phosphorylated Akt.[ ... It has been shown that CD97 regulates localization and degradation of β-catenin.[24] GSK-3β, inhibited in some cancer, ...
... and stabilize beta-catenin.[20] Mutant of presenilin-1 that reduces the ability to stabilize beta-catenin complex leads to ... presenilin-1 was also found to play a role in beta-catenin phosphorylation.[21] Beta-catenin is coupled by presenilin-1 and ... "Presenilin couples the paired phosphorylation of beta-catenin independent of axin: implications for beta-catenin activation in ... beta-catenin binding. • protein binding. • calcium channel activity. • aspartic-type endopeptidase activity. • endopeptidase ...
... and hCGbeta transgenic female mice present with mammary Gland tumors exhibiting characteristics of the Wnt/beta-catenin pathway ... This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 5 subunit of chorionic gonadotropin ( ... The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and ... Chorionic gonadotropin, beta polypeptide 5 is a protein that in humans is encoded by the CGB5 gene. ...
... a Drosophila protein that is homologous to mammalian beta-catenin Armadillo (C++ library), a software library for linear ...
The result is an uncontrolled production of beta-catenin, which regulates cell growth and cell mobility. With uncontrolled beta ... catenin, the cell loses its adhesive properties. As ECs get packed together to create the lining for a new blood vessel, a ...
连环蛋白(英语:Catenin). *Alpha catenin. *Beta catenin *APC ...
"Identification of a Wnt/Dvl/beta-Catenin --, Pitx2 pathway mediating cell-type-specific proliferation during development.". ...
... nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to ... increase beta adrenergic receptors while decreasing alpha adrenergic receptors- which results in increased levels of ... norepinephrine then acting on lipolysis-inducing beta receptors. ...
Holmen SL, Robertson SA, Zylstra CR, Williams BO (2005). "Wnt-independent activation of beta-catenin mediated by a Dkk1-Fz5 ... "A novel frizzled gene identified in human esophageal carcinoma mediates APC/beta-catenin signals". Proc. Natl. Acad. Sci. U.S.A ... "Caveolin is necessary for Wnt-3a-dependent internalization of LRP6 and accumulation of beta-catenin". Dev. Cell. 11 (2): 213-23 ... amyloid-beta binding. • signal transducer activity. • Wnt-protein binding. • protein binding. • protein kinase binding. • ...
... enhances the Wnt/beta-catenin pathway by relieving antagonistic activity of Chibby". Cancer Research. 66 (2): 723-8. PMID ... correlates with Wnt/beta-catenin target genes and aggressive biological behavior in gastric cancer". Clinical Cancer Research. ... interaction with FGFR2 and beta-catenin signaling pathways". International Journal of Cancer. 121 (6): 1265-73. PMID 17520678. ...
Filamin B, beta (FLNB), also known as Filamin B, beta (actin binding protein 278), is a cytoplasmic protein which in humans is ... Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... Takafuta, T; Wu G; Murphy G F; Shapiro S S (Jul 1998). "Human beta-filamin is a new protein that interacts with the cytoplasmic ... Takafuta T, Wu G, Murphy GF, Shapiro SS (1998). "Human beta-filamin is a new protein that interacts with the cytoplasmic tail ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
... nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ...
... inhibits Wnt signaling by promoting TCF4 degradation and disrupting the TCF4/beta-catenin complex". Cellular Signalling. 22 (11 ... DVL is an integral part of the Wnt canonical pathway (β-catenin dependent) and non-canonical pathway (β-catenin-independent).[2 ... These regions mediate protein-protein interactions and help DVL channel signals into either the β-catenin or the β-catenin ... preventing constitutive degradation of β-catenin.[6][7] The prevention of this degradation DVL allows for β-catenin buildup in ...
... β-catenin, RIP140, PCNA, the DNA metabolic enzymes flap endonuclease-1, thymine DNA glycosylase, and Werner syndrome DNA ... Beta-ketoacyl-ACP synthase. *Glyceronephosphate O-acyltransferase. *Lecithin-cholesterol acyltransferase. *Glycerol-3-phosphate ...
"Organized Emergence of Multiple-Generations of Teeth in Snakes Is Dysregulated by Activation of Wnt/Beta-Catenin Signalling". ...
... beta-catenin. It suggests that miR-181 may eradicate HCC. The increased expression of miR-181a in mature T cells increases ...
"Liver-specific beta-catenin knockout mice exhibit defective bile acid and cholesterol homeostasis and increased susceptibility ...
Roles for beta-catenin (part of the Wnt pathway) and appropriate levels of cell death of cortical progenitors have also been ...
"Presenilins interact with armadillo proteins including neural-specific plakophilin-related protein and beta-catenin". J. ... A novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/delta-catenin ... A novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/delta-catenin ... local control of RhoA at the cleavage furrow by the p0071 catenin". Cell Cycle. 6 (2): 122-7. doi:10.4161/cc.6.2.3741. PMID ...
stimulates AC, raises cAMP, stimulates beta catenin and Glycogen synthase kinase 3 ...
... by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid ... Mohamed, O. A.; Clarke, H. J.; Dufort, D (2004). "Beta-catenin signaling marks the prospective site of primitive streak ... Other deficiencies in signaling pathways, such as in Nodal (a TGF-beta protein), will lead to defective mesoderm formation.[9] ... Both formation of the primitive streak and mesenchymal tissue is dependent on the Wnt/β-catenin pathway.[12] Specific markers ...
β-catenin-activated - with exon 3 versus exon 7/8 mutation. *β-Catenin-activated inflammatory - with exon 3 versus exon 7/8 ... as well as in beta-thalassemia and hemochromatosis.[2] ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... Sajid M, Hu Z, Lele M, Stouffer GA (May 2000). "Protein complexes involving alpha v beta 3 integrins, nonmuscle myosin heavy ...
Catenins. *Alpha catenin. *Beta catenin *APC. *Plakoglobin (gamma catenin). *Delta catenin. *GAN ... The talin n-terminal head domain interacts with the membrane-proximal region of the beta(3) cytoplasmic tail". The Journal of ... Calderwood DA, Zent R, Grant R, Rees DJ, Hynes RO, Ginsberg MH (Oct 1999). "The Talin head domain binds to integrin beta ... in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton". The Journal of Biological ...
"Activation of beta-catenin signalling by GSK-3 inhibition increases p-glycoprotein expression in brain endothelial cells". ... For example, PI3K/Akt pathway[18] and Wnt/ β-catenin pathway[20] were reported to positively regulate the expression of P-gp. ...
Catenin-associated Fer and Fyn tyrosine kinases regulate beta-catenin Tyr-142 phosphorylation and beta-catenin-alpha-catenin ... increases beta-catenin-E-cadherin association, and decreases beta-catenin-sensitive transcription". Cancer Res. 61 (4): 1671-7 ... "The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin". ... "A truncated beta-catenin disrupts the interaction between E-cadherin and alpha-catenin: a cause of loss of intercellular ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
MCC inhibits beta-catenin transcriptional activity by sequestering DBC1 in the cytoplasm.. [Laurent Pangon, Dessislava ... In particular, the MCC protein is known to regulate beta-catenin (β-cat) signaling, but the mechanism is poorly understood. ...
Catenin beta-1. FLT3. cyclin-D1. BCL9. PLZF. SALL4. Junction plakoglobin. LEF. RARA. PML. Phosphate. Phosphate. GSK3B. RARA. ... Wnt/beta-catenin Signaling Pathway in Leukemia (Homo sapiens). From WikiPathways. Revision as of 14:19, 24 May 2018 by Mick ... Catenin beta-1. Protein. B4DGU4 (Uniprot-TrEMBL) DKK. GeneProduct. ENSG00000107984 (Ensembl) DKK Not Specified. ETO. ... The Wnt/Beta-catenin pathway mediates the transcription of proteins important for maintenance and growth of hematopoietic stem ...
Furthermore, beta-catenin is expressed prior to the time at which MyoD transcripts can be detected. Expression of beta-catenin ... We show that chick frizzled 1 (Fz1), beta-catenin and Lef1 are expressed during somitogenesis. Lef1 and beta-catenin ... In somite explants, Fz1, beta-catenin and Lef1 are expressed prior to activation of myogenesis in response to Shh and Wnt ... Expression of (beta)-catenin in the developing chick myotome is regulated by myogenic signals ...
In zebrafish, previous loss-of-function studies have not identified an essential role for beta-catenin in dorsal axis ... Wnt signals and their transcriptional effector beta-catenin are required for the development of dorsal axial structures. ... MOs directed against beta-catenin-1 (MO1), by contrast, had no ventralizing effect on wild-type embryos. beta-catenin-2 is thus ... Although the ichabod mutation does not functionally alter the beta-catenin-2 reading frame, the level of maternal beta-catenin- ...
1. What happens when you deplete beta catenin in planarians? -The organism is no longer able to form a posterior side ... remember that all hosts above are treated with beta-catenin RNAi). Neoblast Donor Host Treatment Predicted Phenotype Beta ... You want to know which cell types in a planaria require beta-catenin for post-injury axial determination. Specifically, is beta ... 1. What happens when you deplete beta catenin in planarians? -The organism is no longer able to form a posterior side when it ...
... beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin ... Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g ... Mouse Monoclonal to beta-Catenin. EM-22 (IgG1). Technical Information Request Inquiry for Bulk Prices. ... 3. Flow cytometry analysis (intracellular) of beta-catenin in human MCF-7 cell line by EM-22-Alexa Fluor® 488 (red) compared to ...
Intramyocellular lipid accumulation is related to the down-regulation of Wnt-beta-catenin pathway. Metabolic Syndrome is a ... Six homeoproteins and Wnt-beta-catenin pathway in muscle development and disease * Tweeter ... Six homeoproteins and Wnt-beta-catenin pathway in muscle development and disease ... we plan to determine whether activation of the Wnt-beta-catenin pathway could maintain muscle satellite cells in the myoblastic ...
Unconjugated beta Catenin, clone: 15B8, eBioscience™ Primary Antibodies Cas to Caz ... Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which ... The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and ... More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the ...
Most studies investigating catenin actions focus on alpha catenin and beta catenin. Beta catenin is particularly interesting as ... It belongs to a family of biological compounds known as catenins, consisting of alpha catenin, beta catenin and gamma catenin. ... ROLE OF BETA CATENIN IN THE DEVELOPMENT OF CANCERS:. The same properties of beta catenin that give it an important role in ... Wnt/ beta catenin pathway, also known as canonical Wnt pathway is the Wnt pathway that causes an accumulation of beta catenin ...
Catenin References *↑ Daniels DL, Weis WI. ICAT inhibits beta-catenin binding to Tcf/Lef-family transcription factors and the ... an inhibitor of beta-catenin-mediated transcription, bound to the armadillo repeat domain of beta-catenin, has been determined ... The C-terminal armadillo repeats of beta-catenin may be an attractive target for compounds designed to disrupt aberrant beta- ... catenin-mediated transcription associated with various cancers. ICAT inhibits beta-catenin binding to Tcf/Lef-family ...
... and to determine whether aberrant beta-catenin expression is driven by CTNNB1 (beta-catenin encoding gene) activating mutations ... of the cases displayed lack/reduction of beta-catenin membranous expression and nuclear accumulation. Complete lack of beta- ... beta-Catenin pathway activation in breast cancer is associated with triple-negative phenotype but not with CTNNB1 mutation ... The association of beta-catenin/Wnt pathway activation with clinical outcome and the mechanisms leading to its activation in ...
... beta 1 (88kD); Catenin beta; Catnb; Ctnnb; CTNNB1 Beta-catenin (β-catenin) (Armadillo in Drosophila) is a multifunctional ... Catenin (cadherin-associated protein), beta 1 (88kD); Catenin beta; Catnb; Ctnnb; CTNNB1 ... Beta-catenin (β-catenin) (Armadillo in Drosophila) is a multifunctional protein involved in two essential cellular events: cell ... Clevers H. Wnt/beta-catenin signaling in development and disease. Cell. 2006;127:469-80.PubMedPubMedCentralCrossRefGoogle ...
Beta-catenin (IPR013284). Short name: Beta-catenin Family relationships *Beta-catenin (IPR013284) *Junction plakoglobin/protein ... Beta-catenin forms a cadherin/beta-catenin/alphaE-catenin complex that can tether the tripartite adhesion complex and regulate ... The beta-catenin structure has been determined [PMID: 9298899, PMID: 11136974]. Beta catenin family proteins contain several ... The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis.. ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The degree to which beta-catenin participates in these two functions is dictated by the availability of beta-catenin binding ... Beta-catenin plays a critical structural role in cadherin-based adhesions and is also an essential co-activator of Wnt-mediated ... Phospho-regulation of Beta-catenin adhesion and signaling functions.. Daugherty RL1, Gottardi CJ. ... Inputs from various cell-signaling events can therefore impact beta-catenin function, which may be necessary for the finely ...
Genomic organization of the human beta-catenin gene (CTNNB1).. Nollet F1, Berx G, Molemans F, van Roy F. ... The cytoplasmic beta-catenin protein is implicated in signal transduction and associates with both the cell-cell adhesion ... We determined the primary structure of the human beta-catenin gene (CTNNB1) by analysis of cDNA and genomic clones. The size of ... The intron-exon boundaries did not coincide either with conserved sites in the 12 armadillo repeat sequences of beta-catenin or ...
... have discovered that a single mutation in the beta-catenin gene, which codes a protein known to be deeply involved in a number ... Beta-catenin is an essential protein in the Wnt/beta-catenin signaling pathway, which has been shown in mice to be involved in ... developed a mouse with single mutation to the beta-catenin gene, with the goal to discover so far unrevealed functions of beta- ... "A single mutation in the beta-catenin gene can lead to infertility." Medical News Today. MediLexicon, Intl., 10 Nov. 2014. Web. ...
Beta-catenin is also involved in the regulation of gene expression as a mediator of the Wnt signaling pathway. The expression ... and intracellular localization of beta-catenin is altered in many types of cancers. ... Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding to the ... Clone β-Catenin-1 Beta-catenin is an 88 kDa multifunctional protein playing an essential role in cell-cell adhesion by binding ...
The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. ... BETA-CATENIN A, C 538 Mus musculus Fragment: ARMADILLO DOMAIN Gene Name(s): Ctnnb1 Catnb ...
Loss of functional APC protein results in the accumulation of beta-catenin. Mutant forms of beta-catenin have been discovered ... Loss of functional APC protein results in the accumulation of beta-catenin. Mutant forms of beta-catenin have been discovered ... with which beta-catenin interacts. Here we show that beta-catenin activates transcription from the cyclin D1 promoter, and that ... Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells Nature. 1999 Apr 1;398(6726):422-6. doi: 10.1038/18884 ...
... beta-catenin-TCF7L2 complex, catenin complex, cell cortex, cell junction, cell periphery, cell-cell adherens junction, cell- ... R-SSC-195253. Degradation of beta-catenin by the destruction complex. R-SSC-196299. Beta-catenin phosphorylation cascade. R-SSC ... R-SSC-195253. Degradation of beta-catenin by the destruction complex. R-SSC-196299. Beta-catenin phosphorylation cascade. R-SSC ... Beta-cateninImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href ...
Gene ablation and transgenic expression studies strongly support the concept that beta-catenin together with Lef/Tcf factors ... Beta-catenin regulates cell-cell adhesion and transduces signals from many pathways to regulate the transcriptional activities ... Stabilized beta-catenin induces hyperplasia and mammary tumors in mice. Each of the beta-catenin-induced phenotypes is ... Beta-catenin and Tcfs in mammary development and cancer J Mammary Gland Biol Neoplasia. 2003 Apr;8(2):145-58. doi: 10.1023/a: ...
Michael Davidsons lab contains the insert Beta-Catenin. This plasmid is available through Addgene. ... mEmerald-Beta-Catenin-20 was a gift from Michael Davidson (Addgene plasmid # 54017 ; http://n2t.net/addgene:54017 ; RRID: ...
Catenin beta-1Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href ... tr,D3YUH4,D3YUH4_MOUSE Catenin beta-1 (Fragment) OS=Mus musculus GN=Ctnnb1 PE=1 SV=1 ...
... for Anti-beta Catenin antibody [E247] used in Immunocytochemistry/ Immunofluorescence. Abcam provides excellent in-house ...
Rabbit polyclonal beta Catenin (phospho S45) antibody. Validated in WB. Cited in 1 publication(s). Immunogen corresponding to ... Anti-beta Catenin (phospho S45) antibody. See all beta Catenin primary antibodies. ... The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are ... Synthetic phospho-peptide corresponding to residues surrounding Ser45 of human Beta Catenin. ...
beta Catenin Polyclonal Antibody from Invitrogen for Western Blot, Immunofluorescence, Immunocytochemistry, ... Protein Aliases: beta catenin; Beta-catenin; catenin; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin- ... Catenin beta; Catenin beta-1; CATNB; CTNB1; CTNNB Gene Aliases: armadillo; B-catenin; beta-catenin; Bfc; Catnb; CHBCAT; CTNNB; ... Cite beta Catenin Polyclonal Antibody. The following antibody was used in this experiment: beta Catenin Polyclonal Antibody ...
In this work, the Pourquié team tested the importance of Beta-catenin, a protein that functions as the principal mediator of ... "But, by using the real-time imaging technique in mouse embryos, we could show that increasing Beta-catenin also corresponded ... The Stowers Institutes Pourquié Lab has demonstrated the importance of Beta-catenin, a key component of the Wnt-signaling ... They showed that a newly identified Beta-catenin protein gradient in the PSM is critical in regulating mesoderm maturation. ...
OriGene Anti-Beta-catenin Monoclonal (UMAB15), UltraMAB™, Catalog # UM500015CF. Tested in Western Blot (WB), Immunofluorescence ... beta 1, 88kDa; Catenin b 1; Catenin b1; catenin beta; Catenin beta-1; Catenin beta1; Catenin β 1; Catenin β1; CTNB1; dJ633O20.1 ... Beta-catenin; Betacatenin; C20orf33; catenin; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin- ... The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is ...
... binds to beta-catenin. A chimera consisting of the alpha-catenin-binding region of beta-catenin linked to the amino terminus of ... In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. alpha-catenin is ... In adherens junctions, alpha-catenin links the cadherin-beta-catenin complex to the actin-based cytoskeleton. alpha-catenin is ... Structure of the dimerization and beta-catenin-binding region of alpha-catenin.. Pokutta, S., Weis, W.I.. (2000) Mol Cell 5: ...
View mouse Ctnnb1 Chr9:120933400-120960507 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
... products and learn more about Mouse anti-beta-Catenin, Clone: 12F7, DyLight 405, Novus Biologicals 100 100 Tests; DyLight ... beta 1 (88kD), beta-catenin, catenin (cadherin-associated protein), beta 1, 88kDa, catenin beta-1, CTNNB, DKFZp686D02253, ... beta-Catenin Monoclonal antibody specifically detects beta-Catenin in Human, Mouse, Rat, Chicken, Primate samples. It is ...
... and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin ... and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily ... Polyclonal Antibody Immunofluorescence Immunocytochemistry Beta-Catenin Binding. Polyclonal Antibody - α-E-Catenin Antibody, ... α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to ...
... p120-catenin, beta-catenin, and beta-catenin. The alpha- catenin and beta-catenin then form a complex to link the actin ... THE beta-CATENIN KINETICS We first present our model of the intracellular beta-catenin dynamics and show the importance of this ... We include soluble beta-catenin and the E-cadherin- beta-catenin complex as the main variables of our model. Upregulation of ... beta-catenin is an intracellular protein associated with the actin cytoskeleton of a cell. E- cadherins bind to beta-catenin to ...
Related Wnt/beta-catenin Products. * XAV-939 XAV-939 selectively inhibits Wnt/β-catenin-mediated transcription through ... PRI-724 specifically inhibits the recruiting of beta-catenin with its coactivator CBP.. ... PRI-724 specifically inhibits the recruiting of beta-catenin with its coactivator CBP. ... IWR-1-endo (IC50=180nM) inhibits Wnt-induced stabilization of β-catenin by acting on the target of β-catenin destruction ...
β-catenin is a critical component of the Wnt signaling pathway, which is itself important in embryogenesis, stem cell ... Clone D10A8 recognizes endogenous levels of total β-catenin protein. ... Anti-Beta-Catenin (D10A8)-165Ho-25 µg. Clone D10A8 recognizes endogenous levels of total β-catenin protein. β-catenin is a ... In the absence of Wnt signaling, the kinases CK1 and GSK3β phosphorylate β-catenin at multiple sites, leading to proteasomal ...
... beta-Catenin-independent Wnt/Ca2+ Signaling and Other Non-canonical Wnt Signaling Pathways with our interactive pathway. ... In the absence of Wnt, cytoplasmic beta-Catenin is phosphorylated by CK1 and GSK-3 beta in the beta-Catenin destruction complex ... Wnt Signaling Pathways: beta-Catenin-dependent Wnt Signaling * Wnt Signaling Pathways: beta-Catenin-independent Wnt/Ca2+ ... Inhibition of beta-Catenin-. Dependent Wnt Signaling. Cell Fate/Cell Migration. Inhibition of beta-Catenin-. Dependent Wnt ...
Wnt5-a/b / β-catenin / Axin2; PubMed: 27119499 Oncotarget. A549 cells were harvested, and Western blot analysis was conducted ... β-catenin inhibitor inhibited the expression of MMP7 in Bel7402 cells. Bel7402 cells were treated without or with various ... Western blot for key regulators of the Wnt/β-catenin signaling pathway. The cells were treated with IWP-2 for 3 days. *P,0.05 ... IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. [1] ...
... beta 1(CTNNB1), full length, with N-terminal His tag, expressed in sf9 cells ... Recombinant protein of human human catenin (cadherin-associated protein), ... Recombinant protein of human human catenin (cadherin-associated protein), beta 1(CTNNB1), full length, with N-terminal His tag ... Recombinant protein of human human catenin (cadherin-associated protein), beta 1(CTNNB1), full length, with N-terminal His tag ...
  • 1997 ) -catenin is a target for the ubiquitin-proteasome pathway. (biologists.org)
  • Intramyocellular lipid accumulation is related to the down-regulation of Wnt-beta-catenin pathway. (institutcochin.fr)
  • As Wnt-10b and SREBP-1c proteins showed an inverse expression profile throughout differentiation in cultured satellite cells, we plan to determine whether activation of the Wnt-beta-catenin pathway could maintain muscle satellite cells in the myoblastic lineage, preventing them from expressing adipogenic factors that induce lipid synthesis in skeletal muscle. (institutcochin.fr)
  • Investigation of SREBP-1c regulation by the Wnt-catenin pathway should provide new insights into muscle insulin resistance and open perspectives for the unravelling of cellular mechanisms involved in ectopic lipid deposition in skeletal muscle during obesity and type 2 diabetes. (institutcochin.fr)
  • The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (fishersci.co.uk)
  • β-catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. (wordpress.com)
  • Secondly, beta catenin participates in the 'Wnt signaling pathway' as a downstream target. (wordpress.com)
  • Wnt/ beta catenin pathway, also known as canonical Wnt pathway is the Wnt pathway that causes an accumulation of beta catenin in the cytoplasm and its eventual translocation into the nucleus to act as a transcriptional coactivator of transcription factors that belong to the TCF/LEF family. (wordpress.com)
  • In general, when 'Wnt' is not present, GSK-3B (a member of the pathway) is able to phosphorylate beta catenin as a result of a complex formation that includes beta catenin, AXIN1, AXIN2, APC (a tumor suppressor gene product), CSNK1A1, and GSK3B. (wordpress.com)
  • In particular, the MCC protein is known to regulate beta-catenin (β-cat) signaling, but the mechanism is poorly understood. (sigmaaldrich.com)
  • BETA CATENIN is a protein found as part of molecular complexes in forming cadherin cell adhesion factors of animal cells. (wordpress.com)
  • BETA CATENIN is a dual function protein, regulating the coordination of cell-cell adhesion and gene transcription. (wordpress.com)
  • This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. (nih.gov)
  • This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. (nih.gov)
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as 'adherens junctions' and they typically include at least cadherin, beta catenin, and alpha catenin. (wordpress.com)
  • Most studies investigating catenin actions focus on alpha catenin and beta catenin. (wordpress.com)
  • Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. (fishersci.co.uk)
  • Expression of beta-catenin mRNA is regulated by positive and negative signals derived from neural tube, notochord and lateral plate mesoderm. (biologists.org)
  • Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). (fishersci.co.uk)
  • Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. (fishersci.co.uk)
  • 1994 ) -catenin localization during Xenopus embryogenesis-accumulation at tissue and somite boundaries. (biologists.org)
  • No, Beta-catenin is the downstream transcriptional effector of the Wnt ligand. (coursehero.com)
  • 1996 ) Functional interaction of-catenin with the transcription factor Lef-1. (biologists.org)
  • More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. (fishersci.co.uk)
  • In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. (fishersci.co.uk)
  • 3. You want to know which cell types in a planaria require beta-catenin for post-injury axial determination. (coursehero.com)
  • Their attempts have not been so far successful, since any chemical compound they develop to target BETA CATENIN will inevitably have serious harmful effects upon the organism, since it is an essential biological molecule having diverse roles normal vital processes, and its complete inhibition may lead to be very dangerous consequences. (wordpress.com)
  • If planarians were to specifically required beta-catenin in neoblasts for posterior determination, fill out the predicted phenotypes for these experiments (remember that all hosts above are treated with beta-catenin RNAi). (coursehero.com)
  • You perform the following experiments on hosts that are all irradiated and treated with Beta-catenin RNAi, cutting out a middle segment from each experimental set to assay its ability to regenerate. (coursehero.com)
  • They have been trying to develop drugs that could inhibit the over-expressions and aberrations of BETA CATENIN, which is recognized to be playing a big role in the biochemical processes underlying various types of cancers. (wordpress.com)
  • Thus, Wnt1 could act through beta-catenin on cells in the myotome. (biologists.org)
  • Specifically, is beta-catenin required in neoblasts, non-neoblasts, or all planarian cells? (coursehero.com)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is completely surrounded, beta catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wordpress.com)
  • α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). (cellsignal.com)
  • The cytoplasmatic tail of the E-cadherin molecule binds to the proteins of the catenin family: p120-catenin, beta-catenin, and beta-catenin. (redorbit.com)
  • The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues (1,2,3) bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. (genetex.com)
  • Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. (genetex.com)
  • Addgene: Transformation by Wnt family proteins correlates with regulation of beta-catenin. (addgene.org)
  • In addition, we engineered intracellularly functional anti-β-catenin chromobodies by combining the binding moieties of the nanobodies with fluorescent proteins. (mcponline.org)
  • The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind to the highly conserved tail of the E-cadherin molecule. (leicabiosystems.com)
  • Here we find that, in colorectal cancer cells and in developing mouse forelimbs, BCL9 proteins sustain the action of beta-catenin in a largely PYGO-independent manner. (epfl.ch)
  • Expression and purification of human β-catenin-MBP fusion proteins. (genscript.com)
  • Affinity purification and mass spectrometry were used to identify potential β-catenin interacting proteins in SW480 colon cancer cells. (genscript.com)
  • Recombinant β-catenin constructs were used to co-isolate interacting proteins from stable isotope labelled cells followed by detection using mass spectrometry. (genscript.com)
  • In this study, we demonstrated that the function of E-cadherin was completely abolished in the human gastric cancer cell line HSC-39, despite the high expression of E-cadherin, because of mutations in one of the E-cadherin-associated cytoplasmic proteins, beta-catenin. (asm.org)
  • E-cadherin and beta-catenin are key proteins that are essential in the formation of the epithelial cell layer in the colon but their regulatory pathways that are disrupted in cancer metastasis are not completely understood. (garvan.org.au)
  • ß-catenin is part of a complex of proteins that form adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells ( Hartsock and Nelson 2008 ). (mycancergenome.org)
  • We have examined Wnt-1 function in mammalian cells in which armadillo (beta-catenin and plakoglobin) is known to bind to and regulate cadherin cell adhesion proteins. (rupress.org)
  • The interaction of beta-catenin and LRRFIP1 was also reported for human proteins when they were co-expressed in human embryonic kidney 293T (HEK293T) cells followed by co-immunoprecipitation assay. (reactome.org)
  • BACKGROUND: Wnt signaling is thought to be important in prostate cancer, in part because proteins such as beta-catenin can also affect androgen receptor signaling. (ox.ac.uk)
  • Three different forms of catenin (designated alpha, beta and gamma) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex [ PMID: 7945318 ]. (ebi.ac.uk)
  • On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. (cellsignal.com)
  • The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. (cellsignal.com)
  • Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. (genetex.com)
  • Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. (genetex.com)
  • However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. (genetex.com)
  • Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas. (genetex.com)
  • Cytoplasmic localization of β-Catenin has been demonstrated as a marker of poor outcome in breast cancer patients. (biocare.net)
  • Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. (exbio.cz)
  • We show that RET binds to, and tyrosine phosphorylates, beta-catenin and show that the interaction between RET and beta-catenin can be direct and independent of cytoplasmic kinases, such as SRC. (metabolomicscentre.nl)
  • In U87 cell line, inhibition of β-catenin by siRNA suppressed EGF-induced proliferation, migration, invasiveness, and the expression of EMT activators (Snail and Slug). (dovepress.com)
  • These findings provide evidence that SS induces apoptosis of breast tumor cells through a mechanism involving inhibition of PDE5 and attenuation of oncogenic Wnt/beta-catenin mediated transcription. (aacrjournals.org)
  • β-Catenin inhibition by either small molecule inhibitors or siRNA resulted in a dose-dependent increase of cell proliferation, whereas β-Catenin activation by Wnt-3a had the opposite effects, resulting in a decrease in cell viability. (bu.edu)
  • The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis. (ebi.ac.uk)
  • Beta-catenin and plakoglobin expressi. (ugent.be)
  • Verstraeten B, van Hengel J, Huysseune A. Beta-catenin and plakoglobin expression during zebrafish tooth development and replacement. (ugent.be)
  • We show that Wnt-1 expression results in the accumulation of beta-catenin and plakoglobin. (rupress.org)
  • The degree to which beta-catenin participates in these two functions is dictated by the availability of beta-catenin binding partners, and an emerging theme is that these binding interactions are regulated by phosphorylation. (nih.gov)
  • IWP-2 blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. (selleckchem.com)
  • Tcf3 is a substrate for both glycogen synthase kinase (GSK) 3 and casein kinase (CK) 1epsilon, and phosphorylation of Tcf3 by CKIepsilon stimulates its binding to beta-catenin, an effect reversed by GSK3 . (xenbase.org)
  • Tcf3 blocks β-catenin degradation in extracts and phosphorylation in vitro. (xenbase.org)
  • D) Tcf3 inhibits the phosphorylation of β-catenin by GSK3 and axin in a purified system. (xenbase.org)
  • This prevents phosphorylation of downstream molecules allowing β-catenin association with Tcf/Lef in the nucleus and subsequent increased cell proliferation. (springermedizin.de)
  • As a result of RET -mediated tyrosine phosphorylation, beta-catenin escapes cytosolic down-regulation by the adenomatous polyposis coli/Axin/glycogen synthase kinase-3 complex and accumulates in the nucleus, where it can stimulate beta-catenin-specific transcriptional programs in a RET -dependent fashion. (metabolomicscentre.nl)
  • 2018) Beta-Catenin. (springer.com)
  • The report then estimates 2018-2023 market development trends of Beta Catenin industry. (reportsnreports.com)
  • and Barrett, Terrence A., "Beta-Catenin Cleavage Enhances Transcriptional Activation" (2018). (uky.edu)
  • Catenin Beta 1 - Pipeline Review, H1 2018, outlays comprehensive information on the Catenin Beta 1 targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (whatech.com)
  • Here we show that beta-catenin activates transcription from the cyclin D1 promoter, and that sequences within the promoter that are related to consensus TCF/LEF-binding sites are necessary for activation. (nih.gov)
  • We showed that zygotic transcription of beta-catenin starts after the midblastula transition (MBT), but does not rescue dorsal axial structures. (biologists.org)
  • 1996 ) Functional interaction of-catenin with the transcription factor Lef-1. (biologists.org)
  • Lymphoid enhancer binding factor (Lef) 1 is a Wnt-responsive transcription factor that associates with β-catenin. (asbmr.org)
  • We identified the transcription factor TBX3 as a candidate tissue-specific member of the beta-catenin transcriptional complex. (epfl.ch)
  • Here, we provide evidence that HIC1 antagonizes the TCF/beta-catenin-mediated transcription in Wnt-stimulated cells. (muni.cz)
  • 5 Here, we further investigated the ability of AIEC to activate Wnt transcription and nuclear translocation of b-catenin. (bmj.com)
  • ß-catenin is then free to translocate to the cell nucleus where it acts as a co-factor for the T-cell factor/lymphoid enhancing factor (TCF/LEF) transcription factors. (mycancergenome.org)
  • ß-catenin subsequently translocates to the nucleus, where it acts as a co-factor for the TCF/LEF family of transcription factors. (mycancergenome.org)
  • Suppression of PDE5 with siRNA or known PDE5 inhibitors was sufficient to selectively induce apoptosis and attenuate beta-catenin mediated transcription in breast tumor cells with minimal effects on normal mammary epithelial cells. (aacrjournals.org)
  • Three-dimensional structure of the armadillo repeat region of beta-catenin. (ebi.ac.uk)
  • 12) The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. (genetex.com)
  • E- cadherins bind to beta-catenin to form a complex which can interact both with neighboring cells to form bonds, and with the cytoskeleton of the cell. (redorbit.com)
  • Mechanistically, we establish that MCC interacts with the E-cadherin/beta-catenin complex. (garvan.org.au)
  • A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. (thermofisher.com)
  • Studies from our group indicate that β-catenin activation in colitis and colorectal cancer (CRC) correlates with increased nuclear levels of β-catenin phosphorylated at serine 552 (pβ-Cat 552 ). (uky.edu)
  • Abnormal levels of beta-catenin may therefore contribute to neoplastic transformation by causing accumulation of cyclin D1. (nih.gov)
  • Moreover, the chromobody signal allowed us to trace the accumulation of diffusible, hypo-phosphorylated β-catenin in response to compound treatment in real time using High Content Imaging. (mcponline.org)
  • 1994 ) -catenin localization during Xenopus embryogenesis-accumulation at tissue and somite boundaries. (biologists.org)
  • In zebrafish, previous loss-of-function studies have not identified an essential role for beta-catenin in dorsal axis formation, but the maternal-effect mutation ichabod disrupts beta-catenin accumulation in dorsal nuclei and leads to a reduction of dorsoanterior derivatives. (biomedsearch.com)
  • Immunolabeling for β-catenin confirmed the presence of abnormal nuclear accumulation in SSAs, with 35/54 (67%) SSAs showing nuclear labeling compared to 0/12 hyperplastic polyps (HPs). (pubmedcentralcanada.ca)
  • In Xenopus, Wnt signals and their transcriptional effector beta-catenin are required for the development of dorsal axial structures. (biomedsearch.com)
  • Specificity Recognizes human Catenin, beta. (gentaur.com)
  • Here we investigated, firstly, the role(s) of beta-catenin in spatial terms, in different regions of the embryo, by injecting beta-catenin mRNA into individual blastomeres of beta-catenin-depleted embryos at the 32 cell stage. (biologists.org)
  • Overexpressed in vitro double truncated β-catenin increased transcriptional activity, cell proliferation and growth of tumor xenografts compared to FS β-catenin. (uky.edu)
  • Crystal structure of a beta-catenin/Tcf complex. (ebi.ac.uk)
  • D) Half-life of the axin-β-catenin complex. (xenbase.org)
  • Our work implicates TBX3 as context-dependent component of the Wnt/beta-catenin-dependent transcriptional complex. (epfl.ch)
  • In particular, we identified interaction with a set of coatomer complex I subunits implicated in retrograde transport at the Golgi, and confirmed endogenous interaction of β-catenin with coatomer subunit COPB using immunoprecipitation assays and immunofluorescence microscopy. (genscript.com)
  • The GL treatment resulted in a significant reduction of β-Catenin /TCF-4 complex in both of the cancer cells. (biomedcentral.com)
  • The disruption of E-cadherin/beta-catenin complex formation promotes EMT, thereby stimulating tumor progression. (ox.ac.uk)
  • For the first time, we were able to visualize the subcellular localization and nuclear translocation of endogenous β-catenin in living cells using these chromobodies. (mcponline.org)
  • Nuclear translocation of b-catenin was assessed by immunofluorescence in CRC cell-lines SW480 and DLD1. (bmj.com)
  • Infection of SW480 and DLD1 showed significant increases in b -catenin nuclear translocation as per prostaglandin E2 treatment (1-10 μM). (bmj.com)
  • alanin substitution abrogated K48 polyubiquitination, β-catenin nuclear translocation and tumor xenograft growth. (uky.edu)
  • We selected nanobodies recognizing the N-terminal, core or C-terminal domain of β-catenin and applied these new high-affinity binders as capture molecules in sandwich immunoassays and co-immunoprecipitations of endogenous β-catenin complexes. (mcponline.org)
  • Heteroduplex was used to investigate mutations in beta-catenin. (mdpi.com)
  • The results indicate that beta-catenin can rescue the dorsal axial structures in a non-cell-autonomous way and without changing the fates of the injected cells. (biologists.org)
  • 2001), Physiological regulation of [beta]-catenin stab. (xenbase.org)
  • Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon . (xenbase.org)
  • We show that down-regulation of beta-catenin activity decreases RET -mediated cell proliferation, colony formation, and tumor growth in nude mice. (metabolomicscentre.nl)
  • E7386 disrupted the interaction between beta-catenin and CBP in co-immunoprecipitation assay. (aacrjournals.org)
  • The 79-kDa band was immunologically identified as beta-catenin by using immunoblotting with anti-beta-catenin antibodies. (asm.org)
  • We demonstrate that Tcf3 can inhibit beta-catenin turnover via its competition with axin and adenomatous polyposis for beta-catenin binding. (xenbase.org)
  • The distribution of Wnt2 and beta-catenin in rat corneas was examined by Immunofluorescence staining. (arvojournals.org)
  • To date, β-catenin has been reported to be implicated in mediating the epithelial-mesenchymal transition (EMT) in a variety of human cancers, which can be triggered by EGF. (dovepress.com)
  • When SSAs were further analyzed with respect to the presence or absence of conventional epithelial dysplasia, nuclear β-catenin labeling was seen in 8/27 (29%) SSAs without dysplasia (SSA) but in 27/27 (100%) of SSAs with dysplasia (SSADs) (p=0.000001). (pubmedcentralcanada.ca)
  • Takemaru K-I, Ohmitsu M, Li F-Q. An oncogenic hub: beta-catenin as a molecular target for cancer therapeutics. (springer.com)
  • Molecular cloning reveals alternative splice forms of human alpha(E)-catenin. (ebi.ac.uk)
  • Together, this data demonstrates that the disruption of cadherin-beta-catenin complexes is an important molecular event through which BDNF increases synapse density in cultured hippocampal neurons. (escholarship.org)
  • Biochemical analysis of nuclear extracts from cancer biopsies revealed the existence of low molecular weight (LMW) pβ-Cat 552 , increased to the exclusion of full size (FS) forms of β-catenin. (uky.edu)
  • The different tasks of β-catenin are orchestrated by its subcellular localization and participation in multiprotein complexes. (mcponline.org)
  • Artificially maintaining cadherin-beta-catenin complexes in the presence of BDNF abolishes the BDNF-mediated enhancement of synaptic vesicle mobility, as well as the longer-term BDNF-mediated increase in synapse number. (escholarship.org)
  • Grigoryan T, Wend P, Klaus A, Birchmeier W. Deciphering the function of canonical Wnt signals in development and disease: conditional loss- and gain-of-function mutations of beta-catenin in mice. (springer.com)