Amylases: A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.beta 2-Glycoprotein I: A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antiphospholipid Syndrome: The presence of antibodies directed against phospholipids (ANTIBODIES, ANTIPHOSPHOLIPID). The condition is associated with a variety of diseases, notably systemic lupus erythematosus and other connective tissue diseases, thrombopenia, and arterial or venous thromboses. In pregnancy it can cause abortion. Of the phospholipids, the cardiolipins show markedly elevated levels of anticardiolipin antibodies (ANTIBODIES, ANTICARDIOLIPIN). Present also are high levels of lupus anticoagulant (LUPUS COAGULATION INHIBITOR).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibodies, Anticardiolipin: Antiphospholipid antibodies found in association with systemic lupus erythematosus (LUPUS ERYTHEMATOSUS, SYSTEMIC;), ANTIPHOSPHOLIPID SYNDROME; and in a variety of other diseases as well as in healthy individuals. The antibodies are detected by solid-phase IMMUNOASSAY employing the purified phospholipid antigen CARDIOLIPIN.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Brassicaceae: A plant family of the order Capparales, subclass Dilleniidae, class Magnoliopsida. They are mostly herbaceous plants with peppery-flavored leaves, due to gluconapin (GLUCOSINOLATES) and its hydrolysis product butenylisotrhiocyanate. The family includes many plants of economic importance that have been extensively altered and domesticated by humans. Flowers have 4 petals. Podlike fruits contain a number of seeds. Cress is a general term used for many in the Brassicacea family. Rockcress is usually ARABIS; Bittercress is usually CARDAMINE; Yellowcress is usually RORIPPA; Pennycress is usually THLASPI; Watercress refers to NASTURTIUM; or RORIPPA or TROPAEOLUM; Gardencress refers to LEPIDIUM; Indiancress refers to TROPAEOLUM.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Plant: The functional hereditary units of PLANTS.Self-Incompatibility in Flowering Plants: One of many different processes which occur in ANGIOSPERMS by which genetic diversity is maintained while INBREEDING is prevented.alpha-Cyclodextrins: Cyclic GLUCANS consisting of six (6) glucopyranose units linked by 1,4-glycosidic bonds.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Pancreas: A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.Isoamylase: An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 22.214.171.124) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 126.96.36.199) by its action on glycogen. With EC 188.8.131.52, it produces the activity called "debranching enzyme". EC 184.108.40.206.Saliva: The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.Origanum: A plant genus of the family LAMIACEAE that is the source of a familiar food seasoning.alpha-Amylases: Enzymes that catalyze the endohydrolysis of 1,4-alpha-glycosidic linkages in STARCH; GLYCOGEN; and related POLYSACCHARIDES and OLIGOSACCHARIDES containing 3 or more 1,4-alpha-linked D-glucose units.Ceruletide: A specific decapeptide obtained from the skin of Hila caerulea, an Australian amphibian. Caerulein is similar in action and composition to CHOLECYSTOKININ. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Soccer: A game in which a round inflated ball is advanced by kicking or propelling with any part of the body except the hands or arms. The object of the game is to place the ball in opposite goals.Immobilization: The restriction of the MOVEMENT of whole or part of the body by physical means (RESTRAINT, PHYSICAL) or chemically by ANALGESIA, or the use of TRANQUILIZING AGENTS or NEUROMUSCULAR NONDEPOLARIZING AGENTS. It includes experimental protocols used to evaluate the physiologic effects of immobility.ArchivesFrance: A country in western Europe bordered by the Atlantic Ocean, the English Channel, the Mediterranean Sea, and the countries of Belgium, Germany, Italy, Spain, Switzerland, the principalities of Andorra and Monaco, and by the duchy of Luxembourg. Its capital is Paris.Dementia, Vascular: An imprecise term referring to dementia associated with CEREBROVASCULAR DISORDERS, including CEREBRAL INFARCTION (single or multiple), and conditions associated with chronic BRAIN ISCHEMIA. Diffuse, cortical, and subcortical subtypes have been described. (From Gerontol Geriatr 1998 Feb;31(1):36-44)1,4-alpha-Glucan Branching Enzyme: In glycogen or amylopectin synthesis, the enzyme that catalyzes the transfer of a segment of a 1,4-alpha-glucan chain to a primary hydroxy group in a similar glucan chain. EC 220.127.116.11.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Amylose: An unbranched glucan in starch.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Amylopectin: A highly branched glucan in starch.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Beer: An alcoholic beverage usually made from malted cereal grain (as barley), flavored with hops, and brewed by slow fermentation.Cellulase: An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Trichoderma: A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.Commerce: The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Thermoanaerobacter: A genus of gram-positive, anaerobic bacteria in the family Thermoanaerobacteriaceae. Cultures consist of rods interspersed with coccoid cells.Gram-Positive Asporogenous Rods, Irregular: A group of irregular rod-shaped bacteria that stain gram-positive and do not produce endospores.Ethanol: A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.Glucan 1,4-alpha-Glucosidase: An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4.Bacteria, AnaerobicGlycoside Hydrolases
Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution. (1/94)The crystal structure of beta-amylase from Bacillus cereus var. mycoides was determined by the multiple isomorphous replacement method. The structure was refined to a final R-factor of 0.186 for 102,807 independent reflections with F/sigma(F) > or = 2.0 at 2.2 A resolution with root-mean-square deviations from ideality in bond lengths, and bond angles of 0.014 A and 3.00 degrees, respectively. The asymmetric unit comprises four molecules exhibiting a dimer-of-dimers structure. The enzyme, however, acts as a monomer in solution. The beta-amylase molecule folds into three domains; the first one is the N-terminal catalytic domain with a (beta/alpha)8 barrel, the second one is the excursion part from the first one, and the third one is the C-terminal domain with two almost anti-parallel beta-sheets. The active site cleft, including two putative catalytic residues (Glu172 and Glu367), is located on the carboxyl side of the central beta-sheet in the (beta/alpha)8 barrel, as in most amylases. The active site structure of the enzyme resembles that of soybean beta-amylase with slight differences. One calcium ion is bound per molecule far from the active site. The C-terminal domain has a fold similar to the raw starch binding domains of cyclodextrin glycosyltransferase and glucoamylase. (+info)
The evolution of starch-binding domain. (2/94)Amylolytic enzymes belonging to three distinct families of glycosidases (13, 14, 15) contain the starch-binding domain (SBD) positioned almost exclusively at the C-terminus. Detailed analysis of all available SBD sequences from 43 different amylases revealed its independent evolutionary behaviour with regard to the catalytic domains. In the evolutionary tree based on sequence alignment of the SBDs, taxonomy is respected so that fungi and actinomycetes form their own separate parts surrounded by bacteria that are also clustered according to taxonomy. The only known N-terminal SBD from Rhizopus oryzae glucoamylase is on the longest branch separated from all C-terminal SBDs. The 3-dimensional (3-D) structures of fungal glucoamylase and bacterial CGTase SBDs are compared and used to discuss the interesting SBD evolution. (+info)
Prediction of protein cleavage sites by the barley cysteine endoproteases EP-A and EP-B based on the kinetics of synthetic peptide hydrolysis. (3/94)Hordeins, the natural substrates of barley (Hordeum vulgare) cysteine endoproteases (EPs), were isolated as protein bodies and degraded by purified EP-B from green barley malt. Cleavage specificity was determined by synthesizing internally quenched, fluorogenic tetrapeptide substrates of the general formula 2-aminobenzoyl-P(2)-P(1)-P(1)'-P(2)' 1-tyrosine(NO(2))-aspartate. The barley EPs preferred neutral amino acids with large aliphatic and nonpolar (leucine, valine, isoleucine, and methionine) or aromatic (phenylalanine, tyrosine, and tryptophan) side chains at P(2), and showed less specificity at P(1), although asparagine, aspartate, valine, and isoleucine were particularly unfavorable. Peptides with proline at P(1) or P(1)' were extremely poor substrates. Cleavage sites with EP-A and EP-B preferred substrate sequences are found in hordeins, their natural substrates. The substrate specificity of EP-B with synthetic peptides was used successfully to predict the cleavage sites in the C-terminal extension of barley beta-amylase. When all of the primary cleavage sites in C hordein, which occur mainly in the N- and C-terminal domains, were removed by site-directed mutagenesis, the resulting protein was degraded 112 times more slowly than wild-type C hordein. We suggest that removal of the C hordein terminal domains is necessary for unfolding of the beta-reverse turn helix of the central repeat domain, which then becomes more susceptible to proteolytic attack by EP-B. (+info)
Water stress enhances beta-amylase activity in cucumber cotyledons. (4/94)Cotyledons detached from 4-d-old cucumber (Cucumis sativus L.) seedlings were subjected to water stress (air-drying or PEG-treatment) to examine the effects of the stress on carbohydrate metabolism. Amylolytic activity in the cotyledon was increased about 6-fold by water stress within 1 d. The substrate specificity and the action pattern indicated that beta-amylase is responsible for the activity. Activities of azocaseinase, malate dehydrogenase and triose-phosphate isomerase were not affected by water stress, indicating that the effect of the stress on beta-amylase is rather specific. Cycloheximide-treatment strongly reduced the enhancement of beta-amylase activity. The hypocotyl of cucumber seedlings also exhibited an increase in the enzyme activity when subjected to water stress. The major free sugars in cucumber cotyledons were glucose, fructose, maltose, and sucrose; sucrose being the most abundant. Sucrose content in excised, unstressed cotyledons increased markedly during the incubation. Changes in other free sugars were small compared with that of sucrose. Starch also accumulated in unstressed cotyledons. In stressed cotyledons more sucrose and less starch accumulated than in unstressed ones. Such results were discussed in relation to the enhancement of beta-amylase activity. (+info)
Polymorphism in rice amylases at an early stage of seed germination. (5/94)A polymorphism in rice amylases at an early stage of seed germination is analyzed by zymogram. In non-glutinous cultivars of rice, alpha-amylase isozymes are mainly confirmed in germinating seeds. However, in glutinous cultivars, beta-amylase isozymes, which are not confirmed in nonglutinous cultivars, make up the major part of the total amylase activity and the expression of alpha-amylases are repressed. (+info)
Molecular mimicry of substrate oxygen atoms by water molecules in the beta-amylase active site. (6/94)Soybean beta-amylase (EC 18.104.22.168) has been crystallized both free and complexed with a variety of ligands. Four water molecules in the free-enzyme catalytic cleft form a multihydrogen-bond network with eight strategic residues involved in enzyme-ligand hydrogen bonds. We show here that the positions of these four water molecules are coincident with the positions of four potential oxygen atoms of the ligands within the complex. Some of these waters are displaced from the active site when the ligands bind to the enzyme. How many are displaced depends on the shape of the ligand. This means that when one of the four positions is not occupied by a ligand oxygen atom, the corresponding water remains. We studied the functional/structural role of these four waters and conclude that their presence means that the conformation of the eight side chains is fixed in all situations (free or complexed enzyme) and preserved from unwanted or forbidden conformational changes that could hamper the catalytic mechanism. The water structure at the active pocket of beta-amylase is therefore essential for providing the ligand recognition process with plasticity. It does not affect the protein active-site geometry and preserves the overall hydrogen-bonding network, irrespective of which ligand is bound to the enzyme. We also investigated whether other enzymes showed a similar role for water. Finally, we discuss the potential use of these results for predicting whether water molecules can mimic ligand atoms in the active center. (+info)
Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling. (7/94)To understand the effect of water stress on the remobilization of prestored carbon reserves, the changes in the activities of starch hydrolytic enzymes and sucrose-phosphate synthase (SPS) in the stems of rice (Oryza sativa L.) during grain filling were investigated. Two rice cultivars, showing high lodging-resistance and slow remobilization, were grown in the field and subjected to well-watered (WW, psi(soil)=0) and water-stressed (WS, psi(soil)=-0.05 MPa) treatments 9 d after anthesis (DAA) till maturity. Leaf water potentials of both cultivars markedly decreased during the day as a result of WS treatment, but completely recovered by early morning. WS treatment accelerated the reduction of starch in the stems, promoted the reallocation of prefixed (14)C from the stems to grains, shortened the grain filling period, and increased the grain filling rate. More soluble sugars including sucrose were accumulated in the stems under WS than under WW treatments. Both alpha- and beta-amylase activities were enhanced by the WS, with the former enhanced more than the latter, and were significantly correlated with the concentrations of soluble sugars in the stems. The other two possible starch-breaking enzymes, alpha-glucosidase and starch phosphorylase, showed no significant differences in the activities between the WW and WS treatments. Water stress also increased the SPS activity that is responsible for sucrose production. Both V(limit) and V(max), the activities of the enzyme at limiting and saturating substrate concentrations, were enhanced and the activation state (V(limit)/V(max)) was also increased as a result of the more significant enhancement of V(limit). The enhanced SPS activity was closely correlated with an increase of sucrose accumulation in the stems. The results suggest that the fast hydrolysis of starch and increased carbon remobilization were attributed to the enhanced alpha-amylase activity and the high activation state of SPS when the rice was subjected to water stress. (+info)
Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic beta-amylase from Calystegia sepium (hedge bindweed) rhizomes. (8/94)An abundant catalytically active beta-amylase (EC 22.214.171.124) was isolated from resting rhizomes of hedge bindweed (Calystegia sepium). Biochemical analysis of the purified protein, molecular modeling, and cloning of the corresponding gene indicated that this enzyme resembles previously characterized plant beta-amylases with regard to its amino-acid sequence, molecular structure and catalytic activities. Immunolocalization demonstrated that the beta-amylase is exclusively located in the cytoplasm. It is suggested that the hedge bindweed rhizome beta-amylase is a cytoplasmic vegetative storage protein. (+info)
Glycoside hydrolase family 14
Beta-amylase is present in certain bacteria as well as in plants. Three highly conserved sequence regions are found in all ... while the smaller is formed from two long loops extending from the beta-3 and beta-4 strands of the (beta alpha)8 fold. The ... Beta-amylase is an enzyme that hydrolyzes 1,4-alpha-glucosidic linkages in starch-type polysaccharide substrates so as to ... The 3D structure of a complex of soybean beta-amylase with an inhibitor (alpha-cyclodextrin) has been determined to 3.0A ...
Individuals from populations with a high-starch diet tend to have more amylase genes than those with low-starch diets; Beta- ... After the phosphorylation, the first degrading enzyme, beta-amylase (BAM) can attack the glucose chain at its non-reducing end ... Alpha-amylases are found in plants and in animals. Human saliva is rich in amylase, and the pancreas also secretes the enzyme. ... This process is important in the digestion of starch and is also used in brewing, where amylase from the skin of seed grains is ...
Oyama, T.; Kusunoki, M.; Kishimoto, Y.; Takasaki, Y.; Nitta, Y. (1999). "Crystal structure of beta-amylase from Bacillus cereus ... It has a beta-jelly roll fold, with a groove on the concave surface of one of the beta-sheets. Carbohydrate-binding module ... CBM27 adopts a beta sandwich structure comprising 13 beta strands with a single, small alpha-helix and a single metal atom. ... It has a budded fibronectin type III fold consisting of two beta-sheets, arranged as a beta-sheet sandwich and a bud consisting ...
Beta-amylase (EC 126.96.36.199, saccharogen amylase, glycogenase) is an enzyme with the systematic name 4-alpha-D-glucan ... doi:10.1016/s0096-5332(08)60139-3. "Amylase, Alpha" , I.U.B.: 188.8.131.52,4-α-D-Glucan glucanohydrolase. Beta-amylase at the US ... The optimum pH for β-amylase is 4.0-5.0 Amylase Alpha-amylase Rejzek, M.; Stevenson, C. E.; Southard, A. M.; Stanley, D.; ... glycogen and related polysaccharides and oligosaccharides producing beta-maltose by an inversion. Beta-amylase is found in ...
When beta-amylase breaks down starch, it removes two glucose units at a time, producing maltose. An example of this reaction is ...
Unconverted starch becomes sugar during the last steps of mashing, through the action of alpha and beta amylases. The oldest ... Germination produces a number of enzymes, such as amylase, that can convert the starch naturally present in barley and other ... thorough beta-glucanase rest. Rye is said to impart a spicy, dry flavor to beer. Sorghum and millet are often used in African ... and contains large quantities of beta-glucans compared to other grains; these long-chain sugars can leach out during a mash, ...
Beta) Limit dextrin is the remaining polymer produced by enzymatic hydrolysis of amylopectin with beta amylase, which cannot ... Dextrins can be produced from starch using enzymes like amylases, as during digestion in the human body and during malting and ... produced by hydrolysis of amylopectin with alpha amylase. Highly branched cyclic dextrin is a dextrin produced from enzymatic ...
Brown rice syrup
The enzymes used in the saccharification step are the amylolytic enzyme, beta-amylase (usually derived from Bacillus species) ... Such syrups are high in the simple sugar maltose and low in glucose and fructose, due to the enzymatic action of beta- and ... The enzymes used in the liquefaction step are usually alpha-amylases derived from bacterial or fungal bioreactors (Bacillus ... alpha amylase on starch supplied by the sprouted barley. These enzymes produce large amounts of maltose from starch digestion ...
Research Service scientists are interested in specialized enzymes called serine-class proteases that digest beta-amylases, ...
WRKY transcription factor family
... that recognizes SP8 sequences in the 50 upstream regions of genes coding for sporamin and beta-amylase from sweet potato. Mol. ...
List of MeSH codes (D08)
... amylases MeSH D08.811.277.450.066.050 --- alpha-amylase MeSH D08.811.277.450.066.100 --- beta-amylase MeSH D08.811.277.450.114 ... 4-beta-glucosidase MeSH D08.811.277.450.420.200.600 --- glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 --- ... 4-beta xylanases MeSH D08.811.277.450.950.500 --- xylan endo-1,3-beta-xylosidase MeSH D08.811.277.656.149 --- atp-dependent ... beta-glucanase MeSH D08.811.277.450.420.200.500 --- glucan 1,3-beta-glucosidase MeSH D08.811.277.450.420.200.550 --- glucan 1, ...
This domain is organised as a five-stranded antiparallel beta-sheet. Several alpha-amylases contain an all-beta domain, usually ... See amylase for more uses of the amylase family in general. α-Amylase has exhibited efficacy in degrading polymicrobial ... Medical laboratories will usually measure either pancreatic amylase or total amylase. If only pancreatic amylase is measured, ... and a carboxyl-terminal Greek key beta-barrel domain. Several alpha-amylases contain a beta-sheet domain, usually at the C ...
1] MacGregor, E. A.; Jespersen, H. M.; Svensson, B (1996). "A circularly permuted alpha-amylase-type alpha/beta-barrel ... have a different arrangement than those in α-amylase. The folding characteristics of α-amylase and glucansucrase are still very ... However, glucansucrase is very similar to α-amylase, another sugar-cutting enzyme. Glucansucrase thus has many of the same ... Glucansucrase proteins likely evolved from an amylase enzyme precursor. The two enzymes have similar folding patterns and ...
McCleary, BV (Nov 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase), ... Cellulose 1,4-beta-cellobiosidase, an efficient cellulase Cellulase unit, a unit for quantifying cellulase activity PDB: 1NLR ... The specific reaction involved is the hydrolysis of the 1,4-beta-D-glycosidic linkages in cellulose, hemicellulose, lichenin, ...
Organisms meant to produce enzymes such as beta galactosidase, invertase or other amylases may be fed starch to select for ... Of primary interest among the intracellular components are microbial enzymes: catalase, amylase, protease, pectinase, glucose ...
Glucose Amylase/lipase Urine culture Urinalysis Beta-human chorionic gonadotropin (beta-hCG) Cervical cultures are recommended ...
... they are connected via an alpha 1-4 linkage instead of beta. Starches, particularly amylase, are important in many industries, ... There are also conformations that consist of a mix of alpha helices and beta sheets as well as a beta helixes with an alpha ... Beta is a protein that binds to single stranded DNA and assists homologous recombination by promoting annealing between the ... Cellulose is a polysaccharide made up of beta 1-4 linkages between repeat glucose monomers. It is the most abundant source of ...
... which is then removed by hydrolysis with alpha-amylase. The residual solution usually contains coextracts of hemicelluloses and ... 1160/2011 of the Commission, foodstuffs through which 3 g/day of oat beta-glucan are consumed (1 g of oat beta-glucan per ... Recently, the wound healing effectiveness of both yeast and oat beta-glucan were tested on carp. After 14 days, beta-glucan ... and more recently in low molecular weight oat beta-glucans, but high molecular weight oat beta-glucans are hindered by their ...
Organisms can either by alpha-hemolytic, beta-hemolytic, or gamma-hemolytic (non-hemolytic). Amylases are a group of ... The different classes of amylases are α-amylases, β-amylases, and glucoamylases. The α-amylases hydrolyze starch by randomly ... In humans, amylases are secreted by both the pancreas and salivary glands with both sources of the enzyme required for complete ... Amylase breaks down carbohydrates into mono- and disaccharides, so a starch agar must be used for this assay. Once the bacteria ...
Beta-1 adrenergic receptor
Actions of the β1 receptor include: stimulate viscous, amylase-filled secretions from salivary glands Increase cardiac output ... The beta-1 adrenergic receptor (β1 adrenoreceptor), also known as ADRB1, is a beta-adrenergic receptor, and also denotes the ... Structural similarities of beta 1 and beta 2 receptor subtypes demonstrated by photoaffinity labeling". The Journal of ... Frielle T, Kobilka B, Lefkowitz RJ, Caron MG (Jul 1988). "Human beta 1- and beta 2-adrenergic receptors: structurally and ...
... beta-cyclodextrin glucanotransferase, beta-cyclodextrin glycosyltransferase, gamma-cyclodextrin glycosyltransferase, ... Co-substrate specificity in coupling reactions of Macerans amylase". J. Am. Chem. Soc. 76 (9): 2387-2390. doi:10.1021/ ... DePinto JA, Campbell LL (1968). "Purification and properties of the amylase of Bacillus macerans". Biochemistry. 7 (1): 114-20 ... Other names in common use include Bacillus macerans amylase, cyclodextrin glucanotransferase, alpha-cyclodextrin ...
Other amylase enzymes may convert starch to glucose or to oligosaccharides. Cellulose is first hydrolyzed to cellobiose by ... cellulase and then cellobiose is further hydrolyzed to glucose by beta-glucosidase. Animals such as cows (ruminants) are able ... Malt made from barley is used as a source of β-amylase to break down starch into the disaccharide maltose, which can be used by ...
Kunitz STI protease inhibitor
The active inhibitory site containing the scissile bond is located in the loop between beta-strands 4 and 5 in STI and ETI. The ... and the bifunctional proteinase K/alpha-amylase inhibitor from wheat (PK13) have been solved, showing them to share the same ... Murzin AG, Lesk AM, Chothia C (January 1992). "beta-Trefoil fold. Patterns of structure and sequence in the Kunitz inhibitors ... interleukins-1 beta and 1 alpha and fibroblast growth factors". J. Mol. Biol. 223 (2): 531-43. doi:10.1016/0022-2836(92)90668-A ...
Glucan 1,4-alpha-glucosidase (EC 184.108.40.206, glucoamylase, amyloglucosidase (AMG), gamma-amylase, lysosomal alpha-glucosidase, ... linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose Most forms of ... Tsujisaka, Y.; Fukimoto, J.; Yamamoto, T. (1958). "Specificity of crystalline saccharogenic amylase of moulds". Nature. 181: ... acid maltase, exo-1,4-alpha-glucosidase, glucose amylase, gamma-1,4-glucan glucohydrolase, acid maltase, 1,4-alpha-D-glucan ...
... a soluble fraction of alpha-amylase hydrolyzed oat bran or whole oat flour. Oat beta-glucan is a viscous polysaccharide made up ... 3 linkages or beta-1, 4 linkages. This type of beta-glucan is also referred to as a mixed-linkage (1→3), (1→4)-beta-D-glucan. ... Beta-D-glucans, usually referred to as beta-glucans, comprise a class of indigestible polysaccharides widely found in nature in ... In comparison, the indigestible polysaccharide cellulose is also a beta-glucan, but is not soluble because of its (1→4)-beta-D- ...
List of food additives
... in Earl Grey tea Beta-apo-8'-carotenal (C 30) - color Beta-apo-8'-carotenic acid ethyl ester - color Betanin - color (red) ... improving agent Amylases - flour treatment agent Angelica (Angelica archangelica) Anise - Annatto - color Anthocyanins - color ... Carotenes - color Alpha-carotene - color Beta-carotene - color Gamma-carotene - color Carrageenan - thickener, vegetable gum, ... artificial sweetener beta-cyclodextrin - emulsifier Lemongrass (Cymbopogon citratus, C. flexuosus, and other species) - Damiana ...
Development of intestinal microflora and occurrence of diarrhoea in sucking foals: effects of Bacillus cereus var. toyoi...
The present study was performed on a studfarm under typical field conditions. To minimize effects related to husbandry, feeding, and season, our study took place at one thoroughbred farm, within one foaling period from February to May. According to Lahrssen and Zentek  such a study design is important when working with a limited number of animals. Probiotic dosing and treatment period were in accordance with studies by Jeroch et al. , Jadamus et al. , and Vilà et al. .. Unfortunately some foals were treated with antibiotics during a severe period of illness which may have an impact on the subsequent microbial profile in faeces.. Bacterial microflora in faeces was used as an indication of the effects of B. cereus var. toyoi on the intestinal health of foals. Although faeces might have some limitations for describing the gut ecosystem, by comparing microflora and the digestion process in the colon with faeces in fistulated horses Julliand and Goachet  showed that the faecal ...
RCSB PDB - 1J18: Crystal Structure of a Beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltose...
1J18: Crystal Structure of a Catalytic Site Mutant of beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltopentaose
Sequence Similarity - 1J18: Crystal Structure of a Beta-Amylase from Bacillus cereus var. mycoides Cocrystallized...
1J18: Crystal Structure of a Catalytic Site Mutant of beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltopentaose
Food Poisoning AgentVWR offers slides for the varied purposes of your lab. Prepared and digital microscope slides for educational purposes are featured in an array of fields. General purpose microscope slides and cover glasses are offered as well as cavity, chamber, adhesion, and microarray slides for more specific research needs. Disposable and reusable options of varying thickness and diameter are available and may be purchased in kits, packs, cases, or individually.
Bacillus polymyxa - Bacillus polymyxa Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company, Wholesaler,...
RAJVI ENTERPRISE - Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company, Wholesaler, Retailer, Dealer & Producer of Bacillus polymyxa based in Ahmedabad, India
Preferred for the invention is CBMs of Carbohydrate-Binding Module Family 20. CBMs of Carbohydrate-Binding Module Family 20 suitable for the invention may be derived from glucoamylases of Aspergillus awamori (SWISSPROT Q12537), Aspergillus kawachii (SWISSPROT P23176), Aspergillus niger (SWISSPROT P04064), Aspergillus oryzae (SWISSPROT P36914), from alpha-amylases of Aspergillus kawachii (EMBL#AB008370), Aspergillus nidulans (NCBI AAF17100.1), from beta-amylases of Bacillus cereus (SWISSPROT P36924), or from CGTases of Bacillus circulans (SWISSPROT P43379). Preferred is a CBM from the alpha-amyiase of Aspergillus kawachii (EMBL:#AB008370) as well as CBMs having at least 50%, 60%, 70%, 80% or even at least 90% homology to the CBM of the alpha-amyiase of Aspergillus kawachii (EMBL#AB008370), i.e. a CBM having at least 50%, 60%, 70%, 80% or even at least 90% homology to the amino acid sequence of SEQ ID NO:6. Also preferred for the invention are the CBMs of Carbohydrate-Binding Module Family 20 ...
Further Studies on the Amylolytic System Elaborated by Bacillus polymyxa | Biochemical Society Transactions
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BAM12P兔多克隆抗体(ab18789)可与牛样本反应并经WB, RIA, IHC实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。
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Summary: The characteristic nutritional patterns were determined for seven species or varieties of the genus Bacillus. Six strains of Bacillus cereus var. anthracis grew in a complex but defined amino acid medium + thiamine. Three strains of the insect pathogen B. cereus var. thuringiensis had the same nutritional pattern as the parent species B. cereus and grew in a seven amino acid medium. Of twenty strains of B. firmus, ten grew on the seven amino acid medium + biotin while the growth of five other strains was markedly affected by the addition of thiamine. Two of the remaining five strains required thiamine on first isolation, but soon dispensed with it on subculture, while the other three strains continued to require thiamine. Six strains of B. lentus grew in the seven amino acid medium + biotin + thiamine + urea. When the seven amino acid medium was replaced by the complex amino acid medium three of these strains of B. lentus grew without the addition of urea, and the remaining three strains
Deo, Namita and Natarajan, KA and Somasundaran, P (2001) Mechanisms of adhesion of Paenibacillus polymyxa onto hematite, corundum and quartz. In: International Journal of Mineral Processing, 62 (1-4). pp. 27-39. Natarajan, KA and Deo, Namita (2001) Role of bacterial interaction and bioreagents in iron ore flotation. In: International Journal of Mineral Processing, 62 (1-4). pp. 143-157. Deo, Namita and Natarajan, KA and Rao, Hanumantha K and Forssberg, KSE (1999) Biodegradation of some organic reagents from mineral process effluents. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International Biohydrometallurgy Symposium, JUN 20-23, 1999, Madrid, Spain. Deo, Namita and Vasan, SS and Modak, Jayant M and Natarajan, KA (1999) Selective biodissolution of calcium and iron from bauxite in the presence of Bacillus polymyxa. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International ...
This is the final report for a two-year project conducted between September 1, 2002 and October 31, 2004 by Marta S. Izydorczyk, Grain Research Laboratory.
The experimental investigation of microorganisms capable of producing hydrogen for subsequent fuel cell use is described. The microorganisms Clostridium welchii, Aeromonas hydrophila and Bacillus polymyxa were screened using various substrates. Rate studies with E. coli on maltose and glucose and Pseudomonas sp. (G4A) on formate and glucose were conducted. The manner in which hydrogen is produced demonstrates the desirability of following the course of reaction continually. Full factorial studies, with three factors at two levels, were conducted with several organism-substrate systems. Along with results obtained from previous factional factorial experiments, further definition of the criteria for maximizing hydrogen production has been established. Scale-up studies with E. coli-glucose show that the factors governing laboratory scale studies are applicable to large volume fermenters. (Author)(*FUEL CELLS
Abstract. Ahil is a new winter malting barley cultivar developed by the method of experimental mutagenesis at the Institute of Agriculture, Karnobat, Bulgaria. Biological and agronomic characteristics of the cultivar were also investigated for several years and at the Executive Agency of Varietal Testing, Field Inspection and Seed Control trails in the period 2012 - 2013. Averaged for 6 locations in Bulgaria grain yield of the new cultivar was significantly higher than the yield of standard cultivars with about 5%. At the Institute of Agriculture grain yield was 17% higher than the average standard for the period of 5 years. The cultivar has high winter hardness, lodging resistance, resistance to powdery mildew and medium resistance to brown rust. Ahil has good malting quality
Fertilization programs Malting barley, Malting barley is used for the production of barley malt required in the alcoholic fermentation of beer and whisky. For brewing purposes only malting varieties can be cultivated, which are assessed
MALTINGS AND KILN NUMBER 1, MISTLEY QUAY HANDLING AND FORWARDING COMPANY LIMITED, Mistley - 1074932| Historic England
Maltings. Circa 1896, now empty apart from a small area on 5th floor used as offices for Mistley Quay Handling Company. For Free, Rodwell and Company Ltd. Iron work by J.R.M. Fitch, Lawford Ironworks. Red brick with gault dressings. Double range, grey slate roofs. Kiln to east with tall pyramidal roof. An imposing building presenting 8 storeys to the quayside and 5 storeys to High Street. The Kiln of 5 storeys with roof to full height of the Maltings. The lower storeys act as a buttress to the High Street and are 25 feet wide, the 5 upper storeys 56 feet wide. Kiln of 3 bays, Maltings of.17 bays with pilasters between, 4 dentilled and moulded bands and parapet band; gable ends similar of 2:2 bays with central pilasters extending to gable apices, cornices to apices. Originally with segmental headed louvred 3 light ventilator openings to each bay and storey, 4 C20 windows have been inserted to 5th storey of High Street and Mistley Quay faces, also some loading doors. 3.bays break foward to both ...
Castle Malting - Високоякісний солод для високоякісного пива. Cолодівня знаходиться у м. Бельой, що у Бельгії.
Kang-ho) son gets a tutoring job at a lavish home, the Kim family attempt to take over the Park familys affluent lifestyle.. Korean with English subtitles.. Running time: 132 minutes. Age rating: 15. You can pre-order a delicious homemade curry for just £9 in the Riverside Café ahead of the Wednesday evening film screening.. Pre-show dining served from 90 mins to 30 mins prior to show start.. ...
Canada Malting - Salary - Get a free salary comparison based on job title, skills, experience and education. Accurate, reliable salary and compensation comparisons for Canada
East Kent Goldings is gentle, fragrant, and pleasant. Recognized as having the most typical English aroma with the best flavor historically coming from ...
alpha-Cyclodextrin (α-dextrin, α-cyclodextrin, α-CD, alphadextrin, alpha cyclodextrin (dietary fiber)) is a polysaccharide of six glucose units that are covalently attached end to end via α-1, 4 linkages. This fiber is sold under the brand names, "Alpha-Fibe" and "Calorease". Alpha-Fibe is the original product that contains this unique fiber, introduced to the worldwide nutritional supplement market by Bio-Form Essentials in 2007. Soho Flordis International became the patent Assignee as of March 30, 2012 and launched the brand Calorease in 2013. FBCx is not an ingredient, only an abbreviation, and is not part of the scientific name. The abbreviation FBCx, stands for "Fat Binding Complexer" and can be associated with the brand names, "Alpha-Fibe" and "Calorease". There are two other naturally occurring cyclodextrins, β (beta) - and γ (gamma) -, which contain seven and eight glucose units respectively. In water, these fibers take on a toroid or truncated cone configuration. In aqueous ...
Just one week after the company revealed H1 losses of A$10m after tax and exceptional charges, Joe White Maltings Limited has expressed its concern to become a single track operation, concentrating on its malt activities and divesting its struggling food business. The company did make A$355,000 in pre-tax net profits, but the managing director, Ian Fraser, explained that the earnings produced by the core malting business had been negatively offset by the troubled food division, which necessitated a A$9.04m write off. This meant that the food business would be split into three as the first step of a divestment plan.In a public statement, it was announced that the peanut operation at Kingarot, Joe Whites most saleable business, is to be sold to the Peanut Company of Australia (PCA) pending approval from the Australian Competition and Consumer Commission. Renshaw Foods, another part of Joe White Maltings Limited Food Product Division, has also been sold to Select Harvests Limited. A signed agreement
The capabilities of rice (Oryza sativa L.) and wheat (Triticum aestivum L.) seeds (caryopses) to degrade starchy reserves present in the endosperm tissue were compared under anaerobic conditions. The results showed that rice, a species highly tolerant to anoxia, can readily break down starch under a …
Stay British! The Maltings self catering holiday cottage in Freshwater, Isle of Wight, sleeping 6 people, from £318 per week. Part of the Britain Express travel guide to Isle of Wight.
Date: Fri, 28 Nov 1997 12:27:41 -0500 From: David R. Burley ,Dave_Burley at compuserve.com, Subject: Home malting, cidery beer Brewsters: Keith Manefy says: ,Is the drying phase of malting barley absolutely necessary? Would it be= ,possible to germinate the barley to the required stage and then go ,straight to the brewery for mashing. = It is possible Keith, but then you would be using green malt and get a different result than with cured malt. The old time Dutch and Flemish Brewers used to ( and some may still for historical reasons) dry their malt at room temperature in lofts and call it wind malt. If it is dried quickly, I suspect it will have a very high enzyme content, especially beta amylase and the glucanases and proteases. Check out the HBD archives. Malting and Brewing Science says: Perhaps in the future there will be a market for pale malts that are only hand dry, i.e. contain perhaps 5-8% moisture. = Such materials would be much easier to use than green malt while retaining ...
Saflager S-189 is used by a large number of commercial breweries. Selected for its fairly neutral flavor development, this yeast is recommended for a wi...
150. Gamma-Aminobutyric acid (GABA)-A practical indicator for the detection of heterogeneities during malting?
ABSTRACT: Nowadays, very large malt batches are processed, which frequently leads to heterogeneities within the grain beds. As a result beta-glucanase activities, among others, vary within the batches, thus high beta-glucan concentrations remain unhydrolyzed in parts of the batches and can lead to lautering and filtration problems during the brewing process. The kernels enzyme activities mainly depend on their physiological status. Accordingly, the metabolic status of the seeds corresponds to a reliable marker for detecting heterogeneities in the grain beds and for predicting potential processing problems. Up to now, the Calcofluor method according to Carlsberg has been the only standardized method to determine the kernels homogeneity; however, its results are not very precise. In this study, malting trials under differing conditions were carried out to assess if gamma-aminobutyric acid (GABA) can be used as a reliable marker for determining the physiological status of the kernels. Malting ...
A chain superstructure is formed (bundles of needles) is formed during rapid co-precipitation of alpha-cyclodextrin (cyclic starch w/ glucose) and KAuBr4 in ...
Modules of approx. 100 residues. The granular starch-binding function has been demonstrated in one case. Sometimes designated as starch-binding domains (SBD ...
The granular starch-binding function has been demonstrated in several cases. Interact strongly with cyclodextrins. Often designated as starch-binding domains (SBD ...
Check out this delicious recipe for Apple-Glazed Ham with Sweet Potatoes from Weber-the worlds number one authority in grilling.
Mon Oct 26 17:06:03 2015 bam2cfg.pl] Processing bam: Results_RSN_01926694/kayrotypic.bam [Mon Oct 26 17:06:10 2015 bam2cfg.pl] $recordcounter , $expected_max: 30001 , 30000 [Mon Oct 26 17:06:10 2015 bam2cfg.pl] Closing BAM file [Mon Oct 26 17:06:10 2015 bam2cfg.pl] Send TERM signal for 28546 [Mon Oct 26 17:06:12 2015 bam2cfg.pl] samtools pid process 28546 is still there... [Mon Oct 26 17:06:12 2015 bam2cfg.pl] invoking kill -9 on 28546 ... [Mon Oct 26 17:06:12 2015 bam2cfg.pl] Closing samtools process : 28546 [Mon Oct 26 17:06:13 2015 bam2cfg.pl] Processing bam: Results_RSN_01926692/kayrotypic.bam [Mon Oct 26 17:06:21 2015 bam2cfg.pl] $recordcounter , $expected_max: 30001 , 30000 [Mon Oct 26 17:06:21 2015 bam2cfg.pl] Closing BAM file [Mon Oct 26 17:06:21 2015 bam2cfg.pl] Send TERM signal for 28563 [Mon Oct 26 17:06:23 2015 bam2cfg.pl] samtools pid process 28563 is still there... [Mon Oct 26 17:06:23 2015 bam2cfg.pl] invoking kill -9 on 28563 ... [Mon Oct 26 17:06:23 2015 bam2cfg.pl] Closing ...
Dinner got an upgrade with Zemas Panfried Sweet Potato Pancakes, delicious and nutritious, a perfect addition to an family night....
The producer, the person who owns the sweet potatoes immediately after harvest is liable to pay the sweet potatoes levy and charge.. If you produce sweet potatoes and sell your sweet potatoes by retail sale you must lodge a return and make a payment to the department.. If you produce sweet potatoes and sell your sweet potatoes through an intermediary, including a first purchaser, buying agent, selling agent, exporting agent or processor, the amount of levy or charge they pay to the department on your behalf can be recovered from you by offset or otherwise.. If you are an intermediary, including a first purchaser, buying agent, selling agent, exporting agent or processor, you must lodge a return to the department and make a payment. You can recover from the producer the amount of levy or charge paid to the department by offset or otherwise.. If you export sweet potatoes-that is you own the sweet potatoes at the time of export, you must lodge a return to the department and make a payment. ...
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The most commonly used type 2 diabetes treatment is diet modification, although many diabetics rely on insulin pills or shots to keep their glucose level regulated. A less common treatment is a very restrictive diet with a high concentrate of vegetables with low starch levels that appears to reverse type 2 diabetes. Medical experts are also exploring the possibility of curing type 2 diabetes with new medication; however, the FDA has not yet approved the drug.. Some diabetics have been able to regulate their diseases simply by testing their blood sugar levels multiple times each day. Not all diabetics need [Read more…] ...
sweet potato pellets,complete details about sweet potato pellets provided by Junan Dehong Trading Co., Ltd.. You may also find other latest sweet potato pellets selling and buying leads on weiku.com.
Sweet potatoes are most commonly known as a side dish at Thanksgiving dinner, but they can be incorporated into dozens of tasty dishes throughout the year (although November and December hold the root in its prime). Sweet potatoes are nutritious, high in antioxidants, vitamin A (giving it an orange color from ...
Learn more about how sweet potatoes have an impressive nutritional profile and the benefits to adding sweet potatoes to your diet.
They are easily available. They are inexpensive. And they are delicious. Whether it is in casseroles or pies or even fries, sweet potatoes are a common addition to our diet.
A big bowl of creamy soup makes for a perfectly comforting lunch or dinner on a chilly day. Rather than calling for loads of butter or cream, this sweet potato soup recipe is pureed to achieve a similarly silky texture.
A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour - omicX
Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was stimulated in the presence of Mg+2, Ca+2, EDTA, K+, Ba+2 and Na+ and inhibited in the presence of Hg+2, Cu+2, Fe+2 and Zn+2. The results showed that Bacillus sp. SM-02 have good potential for β-CGTase production.
Are sweet potatoes healthy? Were giving you the scoop on sweet potato nutrition, how to cook sweet potatoes, and our favorite sweet potato recipes.
Picture of Sweet Potato Fries - Oven-baked sweet potato fries, with a... csp4233747 - Search Photo Clipart
Sweet Potato Fries Stock Photo. csp4233747 - Oven-baked sweet potato fries, with a dipping sauce. . Affordable Royalty Free Stock Photography. Downloads for just $2.50, with thousands of images added daily. Subscriptions available for just $39.00. Our stock photo image search engine contains royalty free photos, vector clip art images, clipart illustrations.
Curried Sweet Potato Salad is a fabulously flavorful spin on a classic potato salad. Made with the more nutrient-rich sweet potato and paired with a savory curried yogurt dressing, this potato salad is both nutritious and tasty.
Curried Sweet Potato Salad is a fabulously flavorful spin on a classic potato salad. Made with the more nutrient-rich sweet potato and paired with a savory curried yogurt dressing, this potato salad is both nutritious and tasty.
Sweet Potato, the tubular funny looking orange potato, might be your key to weight loss, serving options, recipes, other names for sweet potato, nutrition table, health benefits.
Full nutritional breakdown of the calories in Baked sweet potato fries based on the calories and nutrition in each ingredient, including Sweet potato, cooked, baked in skin, without salt and the other ingredients in this recipe.
Incorporates a phase consisting of a 2,6-di-O-pentyl-3-trifluoroacetyl derivative of alpha-cyclodextrin. Exhibits high selectivity for oxygen containing analytes in the form of alcohols, ketones, acids, aldehydes, and lactones. Highly selective for halogenated compounds.
We are teaming up with Artisan Kitchens to provide easy, healthier recipes for some classic Thanksgiving sides.. Check out the full recipe for Bourbon-Molasses Sweet Potatoes.. ...
The sweet potato casserole you make for the holidays is kind of a production, right? This ones a quick weeknight side dish-ready in less than 20 minutes.
This delicious sweet potato recipe is a great side dish to serve during the holidays. Freeze the leftovers into individual servings.
Growing sweet potatoes - a great storage crop for those looking to be food self-sufficient - is simple in many climates if you follow our tips.
Sweet potato vs yam? Is there a difference? Is one tastier? Is one healthier? Or are they the same freaking thing? Find out here!
Sweet potatoes are a surprisingly nutritious vegetable. They are especially high in vitamins A, C, E, and B6, fiber and manganese.
Date: Thu, 31 Mar 2005 09:46:56 -0500 From: Jeff Renner ,jeffrenner at comcast.net, Subject: Re: Malted Oats and Grain Questions Art McGregor ,A.L.McGregor at verizon.net, writes from Northern Virginia ,Do malted oats need to be mashed with other enzymatic malts, or are they ,sufficiently converted during the malting process to allow me to just steep ,the grains to get the fermentables out? They might actually have enough enzymes, but you might well have some other issues such as slow runoff. Here is a note I posted a while back http://www.hbd.org/hbd/archive/4489.html#4489-6 I wrote Fawcett Malting http://www.fawcett-maltsters.co.uk, the producer of malted oats that are available in the US, about this product, and got this reply from James Fawcett (parts 1-3 were on brown and amber malt): 3) Oat malt laboratory worts are normally slightly hazy rather than clear. Oats are not normally low in protein and are quite difficult to modify from a protein viewpoint, which could well explain this. Our ...
The Thanksgiving spread was just too tempting, and you ate three helpings of Grandmas sweet potato casserole, or maybe you spent the evening at the...
Thermoanaerobacter thermohydrosulfuricus (Klaushofer and Parkkinen) Le
... alcohol ethanol Produces glucoamylase Produces pullulanase pullulan 6-glucanohydrolase Production of thermostable beta-amylase ... Production of thermostable beta-amylase, glucoamylase and pullulanase during the conversion of starch to ethanol ... Degrades starch Produces beta-amylase Produces ethyl ...https://www.atcc.org/Products/All/53033.aspx?p=1&rel=%7B0%7D
Beta-amylase - Oryza sativa (Rice)
PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. ... PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit ... Beta-amylaseUniRule annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... tr,Q42989,Q42989_ORYSA Beta-amylase OS=Oryza sativa OX=4530 PE=3 SV=1 ...http://www.uniprot.org/uniprot/Q42989
Anti-beta Amylase antibody (HRP) | Abcam
Anti-beta Amylase antibody conjugated to HRP validated for WB, ELISA, Dot, EM. Immunogen corresponding to full length native ... Anti-beta Amylase antibody (HRP) - Azide free. See all beta Amylase primary antibodies. ... Beta Amylase is an enzyme found in the seeds of higher plants and in sweet potatoes. It hydrolyses 1,4 alpha D glucosidic ...http://www.abcam.com/beta-amylase-antibody-hrp-azide-free-ab34582.html
RCSB PDB - 1B90: BACILLUS CEREUS BETA-AMYLASE APO FORM
... and beta =101.95 degrees. The structures of free and maltose-bound beta-amylases were determined by X-ray crystallography at ... and beta =101.95 degrees. The structures of free and maltose-bound beta-amylases were determined by ... ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ...https://www.rcsb.org/structure/1b90
Downregulation of a chloroplast-targeted beta-amylase leads to a starch-excess phenotype in leaves. - PubMed - NCBI
Downregulation of a chloroplast-targeted beta-amylase leads to a starch-excess phenotype in leaves.. Scheidig A1, Fröhlich A, ... Expression of the gene in E. coli showed that the protein product was a functional beta-amylase that could degrade both starch ... Analysis of plants reduced in the presence of this beta-amylase isoform showed that their leaves had a starch-excess phenotype ... One clone isolated had a sequence very similar to a recently described chloroplast-targeted beta-amylase of Arabidopsis. ...https://www.ncbi.nlm.nih.gov/pubmed/12047632?dopt=Abstract
ARALYDRAFT 356484 - Beta-amylase - Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) - ARALYDRAFT 356484 gene & protein
tr,D7MUR1,D7MUR1_ARALL Beta-amylase OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_356484 PE=3 SV=1 ...https://www.uniprot.org/uniprot/D7MUR1
Structure Cluster - 1J10: beta-amylase from Bacillus cereus var. mycoides in complex with GGX 3D Similarity Report...
Crystal Structures of beta-Amylase from Bacillus cereus var. mycoides in Complexes with Substrate Analogs and Affinity-Labeling ... Description: Beta-amylase protein , Length: 516 No structure alignment results are available for 1J10.A, 1J10.B, 1J10.C, 1J10.D ...http://www.rcsb.org/pdb/explore/structureCluster.do?structureId=1J10
Inactive beta-amylase elisa and antibody
Recombinant Protein and Inactive beta-amylase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are ... Inactive beta-amylase 4. Inactive beta-amylase 4 ELISA Kit. Inactive beta-amylase 4 Recombinant. Inactive beta-amylase 4 ... Inactive beta-amylase 9. Inactive beta-amylase 9 ELISA Kit. Inactive beta-amylase 9 Recombinant. Inactive beta-amylase 9 ... Also known as Inactive beta-amylase 4, chloroplastic (Inactive beta-amylase 6).. In vitro assay indicates no beta-amylase ...https://www.mybiosource.com/protein_family.php?root=inactive-beta-amylase
Pancreatic Amylase Beta RNAi: Novus Biologicals
Browse our Pancreatic Amylase Beta RNAi catalog backed by our Guarantee+. ... Pancreatic Amylase Beta RNAi available through Novus Biologicals. ... Alternate Names for Pancreatic Amylase Beta RNAi. Pancreatic Amylase Beta RNAi, AMY2B RNAi, alpha-amylase 2B RNAi, AMY2 RNAi, ... We offer Pancreatic Amylase Beta RNAi for use in common research applications. Each Pancreatic Amylase Beta RNAi is fully ...https://www.novusbio.com/rnai/pancreatic-amylase-beta
Alpha and Beta Amylase
... Important enzymes in brewing beer and liquor made from sugars derived from starch. Different ... temperatures optimize the activity of alpha or beta amylase, resulting in different mixtures of fermentable and unfermentable ...https://justbeerapp.com/article/alpha-and-beta-amylase
beta Amylase Proteins,recombinant | Sino Biological
beta Amylase recombinant proteins are produced in house and quality guaranteed.All the beta Amylase recombinant proteins are in ... Among these beta Amylase proteins, there are 2 Human beta Amylase protein. All these beta Amylase protein are expressed by ... Human beta Amylase Protein, Recombinant (2). beta Amylase Protein, Human, Recombinant (His Tag) 13575-H08H Expression host: ... beta Amylase Protein Background. There are 2 beta Amylase protein produced in house with high quality which are covering ...http://fr.sinobiological.com/AMY2B-protein-m01858.html
St. Patty's Post: Beerzymes (Alpha & Beta Amylase)
Not all amylases are the same, alpha and beta amylases perform similar but different jobs. Alpha amylases hydrolyze [HI-dro- ... Alpha-amylase functions at temperatures between 145° to 158° F, while beta-amylase operates between 131° and 149° F.2 Therefore ... At the end of the mashing period, the wort contains (from the alpha-amylase) and maltose (from the beta-amylase), two principal ... you would run past the beta-amylases optimal temperature and mash within alpha-amylases optimal temperature range. ...https://bioconjugate.blog/2017/03/21/st-pattys-post-beerzymes-alpha-beta-amylase/
The Immobilization of a Chemically Thermostabilized Beta-amylase | DIAL.pr - BOREAL
The Immobilization of a Chemically Thermostabilized Beta-amylase.In: Archives Internationales de Physiologie et de Biochimie, ... The Immobilization of a Chemically Thermostabilized Beta-amylase Primary tabs. *Détail(active tab) ...https://dial.uclouvain.be/pr/boreal/object/boreal:64463
"Tetrameric Structure of Beta-amylase 2 (BAM2) in Arabidopsis thaliana" by Lauren Pope
This process of transitory starch degradation is mostly attributable to the β-Amylase (BAM) family, which are starch ... Pope, Lauren, "Tetrameric Structure of Beta-amylase 2 (BAM2) in Arabidopsis thaliana" (2017). Senior Honors Projects, 2010- ... This process of transitory starch degradation is mostly attributable to the β-Amylase (BAM) family, which are starch ...http://commons.lib.jmu.edu/honors201019/506/
Which kits can be used to measure beta-amylase and alpha-amylase? : Megazyme
Which kits can be used to measure beta-amylase and alpha-amylase? Modified on: Fri, 22 Apr, 2016 at 12:57 PM ... The K-MALTA contains 50 assays each of K-CERA and K-BETA3 so measures beta- and alpha-amylase activity (separately). ... K-AMYLSD can be used for measuring alpha-amylase in barley samples (the Amylase SD assay is more sensitive than the K-CERA). ... Knowledge Base Home FAQs ,, FAQs Assay Kits & Reagents α-Amylase SD Assay Kit (High Sensitivity Method) (K-AMYLSD) ...https://support.megazyme.com/support/solutions/articles/8000032593/thumbs_up
Alpha-amylase/branching enzyme, C-terminal all beta (IPR006048) | InterPro | EMBL-EBI
Alpha-amylase/branching enzyme, C-terminal all beta (IPR006048). Short name: A-amylase/branching_C ... Alpha-amylase/branching enzyme, C-terminal all beta (IPR006048) *Alpha-amylase, C-terminal domain (IPR031319) ... Evolution of alpha-amylases: architectural features and key residues in the stabilization of the (beta/alpha)(8) scaffold.. Mol ... such as alpha-amylases and 1,4-alpha-glucan branching enzyme. This domain forms a Greek key beta-barrel fold in these enzymes [ ...http://www.ebi.ac.uk/interpro/entry/IPR006048
Literature: Alpha-amylase/branching enzyme, C-terminal all beta (IPR006048) | InterPro | EMBL-EBI
Literature: Alpha-amylase/branching enzyme, C-terminal all beta (IPR006048). References used in this entry. The following ... Evolution of alpha-amylases: architectural features and key residues in the stabilization of the (beta/alpha)(8) scaffold.. ... Crystal structure of yellow meal worm alpha-amylase at 1.64 A resolution.. Strobl S, Maskos K, Betz M, Wiegand G, Huber R, ... Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase.. Qian M ...http://www.ebi.ac.uk/interpro/entry/IPR006048/literature
Beta-amylase. One.Product presentation This enzyme is also called High Malt-sugar Enzyme.It is a new product produced according ... Fungal Alpha-amylase. One.Product presentation Fungal Alpha-amylase is extracted deeply from the Aspergillus oryzalvar which is ... Middle-temperature Alpha-amylase. One.Product presentation Middle-temperature alpha-amylase is extracted from fermented deeply ... Beta-dextranase. One.Prodcut presentation Beta-dextranase is made by liquid fermentation deeply adopting Bacillus Licheniformis ...http://trademetro.net/catalog/Electronics-and-Electrical-Components-0-320000.html
Some aspects of the joint action of alpha- and beta-malt amylases on startch, with particular reference to the brewery mash
... dc ... Some aspects of the joint action of alpha- and beta-malt amylases on startch, with particular reference to the brewery mash. en ...https://www.ros.hw.ac.uk/handle/10399/2002?show=full
Diel rewiring and positive selection of ancient plant proteins enabled evolution of CAM photosynthesis in Agave | SpringerLink
AGP16, Arabinogalactan protein 16; AKT2, Arabidopsis Shaker family K+ channels 2/3; CT-BMY, Chloroplast Beta-Amylase; ELF3, ... and a chloroplast beta-amylase (CT-BMY).. Open image in new window. ... Additional file 13: Figure S3. Positive selection region in chloroplast β-amylase (CT-BMY). (PDF 171 kb) ... Beta vulgaris (PLAZA 3.0), Brachypodium distachyon (PLAZA 3.0), Carica papaya (PLAZA 3.0), Citrus sinensis (PLAZA 3.0), ...https://link.springer.com/article/10.1186%2Fs12864-018-4964-7
New Arabidopsis DNA 08-Aug-1998
... putative beta-amylase; F4I18.15; F4I18.16; F4I18.17; putative DNA-binding protein; F4I18.18; F4I18.19; F4I18.20; DNA-binding ... beta-glucosyltransferase; F12L6.30; putative beta-1,3-endoglucanase; F12L6.31; F12L6.32; putative protein kinase. ATAC004665 ... geranylgeranyl transferase type I beta subunit; F12L6.22; F12L6.23; F12L6.24; F12L6.25; putative ribosomal protein S15a; ...http://www.bio.net/bionet/mm/arab-gen/1998-August/006547.html
More thermostable than beta-amylase. The brewer controls the relative activities of alpha and beta amylase using mash ... Beta-amylase. In brewing, a malt enzyme that hydrolyses alpha-1,4 bonds in starch. Largely responsible for maltose production ... "exo" activity) Less thermostable than alpha-amylase. The brewer controls the relative activities of alpha and beta amylase ... Alpha Amylase. In brewing, a malt enzyme that hydrolyses alpha-1,4 bonds in starch. Largely responsible for mash viscosity ...https://www.summitbrewing.com/glossary/
Products by Enzyme Type
Barley Beta-Amylase L. Type: Carbohydrase. Origin: Barley. Description: Releases successive maltose units from the non-reducing ... Beta-Glucanase. Type: Carbohydrase. Origin: Trichoderma reesei. Description: Hydrolyzes the β1,3-gylocsidic bonds found in beta ... AllEnzyme Type: AmylasesEnzyme Type: CarbohydrasesEnzyme Type: LipasesEnzyme Type: OtherEnzyme Type: Proteases ... Fungal Amylase. Type: Carbohydrase. Origin: Aspergillus oryzae. Description: Randomly hydrolyzes glycosidic bonds within the ...https://www.bio-cat.com/products-enzyme/
HOMEBREW Digest #5622 Tue 27 October 2009
What about beta amylase? Would you need that as well? Brew on! Doug Moyer Troutville, VA Star City Brewers Guild: http://www. ... alpha amylase? According to wikipedia, Beano contains the enzyme alpha galactosidase. Is this the same as the alpha amylase ... Contents: Beano - alpha amylase? (Doug Moyer) (Tannet-Group Limited) ...http://hbd.org/hbd/archive/5622.html
KEGG BRITE: KEGG Orthology (KO) - Cavenderia fasciculata (cellular slime mold)
... alpha-amylase [EC:220.127.116.11] K01177 E18.104.22.168; beta-amylase [EC:22.214.171.124] K00705 malQ; 4-alpha-glucanotransferase [EC:126.96.36.199] ... 4-beta-cellobiosidase [EC:188.8.131.52] K01225 CBH1; cellulose 1,4-beta-cellobiosidase [EC:184.108.40.206] K18447 NUDX14; ADP-sugar ... DFA_08758 gluA; beta glucosidase DFA_01496 dcsA; cellulose synthase DFA_04566 galactose-binding domain-containing protein DFA_ ... DFA_10059 amyA; putative alpha-amylase DFA_03928 hypothetical protein DFA_01299 4-alpha-glucanotransferase DFA_03630 tpsA; ...http://www.genome.jp/kegg-bin/get_htext?dfa00001+DFA_00663
- The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b = 92.87 A, c = 65.93 A, and beta =101.95 degrees. (rcsb.org)
- 1J10: beta-amylase from Bacillus cereus var. (rcsb.org)
- Crystal structure of calcium-depleted Bacillus licheniformis alpha-amylase at 2.2 A resolution. (ebi.ac.uk)
- Beta Amylase is an enzyme found in the seeds of higher plants and in sweet potatoes. (abcam.com)
- The enzyme consists of a core (beta/alpha)8-barrel domain (residues 5-434) and a C-terminal starch-binding domain (residues 435-613). (rcsb.org)
- Structural representation of the enzyme beta-amylase from barley. (bioconjugate.blog)
- This entry represents the all-beta C-terminal domain that is found in members of the glycosyl hydrolase 13 family, such as alpha-amylases and 1,4-alpha-glucan branching enzyme. (ebi.ac.uk)
- Alpha-amylase is an essential enzyme in alpha-glucan metabolism, acting to catalyse the hydrolysis of alpha-1,4-glucosidic bonds of glycogen, starch and related polysaccharides. (ebi.ac.uk)
- Amylases have at least one conserved calcium-binding site, as calcium is essential for the stability of the enzyme. (ebi.ac.uk)
- The chloride-binding functions to activate the enzyme, which acts by a two-step mechanism involving a catalytic nucleophile base (usually an Asp) and a catalytic proton donor (usually a Glu) that are responsible for the formation of the beta-linked glycosyl-enzyme intermediate. (ebi.ac.uk)
- Beta-amylase is an enzyme that hydrolyzes 1,4-alpha-glucosidic linkages in starch-type polysaccharide substrates so as to remove successive maltose units from the non-reducing ends of the chains. (wikipedia.org)
- The enzyme folds into large and small domains: the large domain has a (beta alpha)8 super-secondary structural core, while the smaller is formed from two long loops extending from the beta-3 and beta-4 strands of the (beta alpha)8 fold. (wikipedia.org)
- The structure of the core domain is similar to that of soybean beta-amylase except for the L4 maltose-binding site, whereas the C-terminal domain has the same secondary structure as domain E of cyclodextrin glucosyltransferase. (rcsb.org)
- The 3D structure of a complex of soybean beta-amylase with an inhibitor (alpha-cyclodextrin) has been determined to 3.0A resolution by X-ray diffraction. (wikipedia.org)
- There are 2 beta Amylase protein produced in house with high quality which are covering various species. (sinobiological.com)
- Among these beta Amylase proteins, there are 2 Human beta Amylase protein. (sinobiological.com)
- All these beta Amylase protein are expressed by different host cells. (sinobiological.com)
- K-AMYLSD can be used for measuring alpha-amylase in barley samples (the Amylase SD assay is more sensitive than the K-CERA). (megazyme.com)
- Due to the usually higher level of higher alpha-amylase in barley grains the only suggested alteration to the standard procedure is that 0.5 g of milled barley grain is extracted in 25 mL of extraction buffer (instead of 0.5 g extracted in 4 mL). (megazyme.com)
- We offer Pancreatic Amylase Beta RNAi for use in common research applications. (novusbio.com)
- Each Pancreatic Amylase Beta RNAi is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
- Our Pancreatic Amylase Beta RNAi can be used in a variety of model species: Human. (novusbio.com)
- Choose from our Pancreatic Amylase Beta RNAi. (novusbio.com)
- Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase. (ebi.ac.uk)
- Refined molecular structure of pig pancreatic alpha-amylase at 2.1 A resolution. (ebi.ac.uk)
- The structures of free and maltose-bound beta-amylases were determined by X-ray crystallography at 2.1 and 2.5 A with R-factors of 0.170 and 0.164, respectively. (rcsb.org)
- At the end of the mashing period, the wort contains (from the alpha-amylase) and maltose (from the beta-amylase), two principal sugars used in fermentation. (bioconjugate.blog)
- Evolution of alpha-amylases: architectural features and key residues in the stabilization of the (beta/alpha)(8) scaffold. (ebi.ac.uk)
- Structure of a complex of Thermoactinomyces vulgaris R-47 alpha-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft. (ebi.ac.uk)
- This is the first time that a physiological role for a beta-amylase in plants has been demonstrated. (nih.gov)
- Alpha-amylase is classified as family 13 of the glycosyl hydrolases and is present in archaea, bacteria, plants and animals. (ebi.ac.uk)
- Beta-amylase is present in certain bacteria as well as in plants. (wikipedia.org)