A drug-metabolizing, cytochrome P-448 (P-450) enzyme which catalyzes the hydroxylation of benzopyrene to 3-hydroxybenzopyrene in the presence of reduced flavoprotein and molecular oxygen. Also acts on certain anthracene derivatives. An aspect of EC 1.14.14.1.
A class of chemicals that contain an anthracene ring with a naphthalene ring attached to it.
A central nervous system stimulant. It was formerly used in the treatment of barbiturate overdose but is now considered to be of no value for such purposes and may be dangerous. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1229)
An enzyme of the oxidoreductase class that catalyzes the formation of L-TYROSINE, dihydrobiopterin, and water from L-PHENYLALANINE, tetrahydrobiopterin, and oxygen. Deficiency of this enzyme may cause PHENYLKETONURIAS and PHENYLKETONURIA, MATERNAL. EC 1.14.16.1.
An adrenal microsomal cytochrome P450 enzyme that catalyzes the 21-hydroxylation of steroids in the presence of molecular oxygen and NADPH-FERRIHEMOPROTEIN REDUCTASE. This enzyme, encoded by CYP21 gene, converts progesterones to precursors of adrenal steroid hormones (CORTICOSTERONE; HYDROCORTISONE). Defects in CYP21 cause congenital adrenal hyperplasia (ADRENAL HYPERPLASIA, CONGENITAL).
An enzyme that catalyzes the hydroxylation of TRYPTOPHAN to 5-HYDROXYTRYPTOPHAN in the presence of NADPH and molecular oxygen. It is important in the biosynthesis of SEROTONIN.
An enzyme that catalyzes the conversion of L-tyrosine, tetrahydrobiopterin, and oxygen to 3,4-dihydroxy-L-phenylalanine, dihydrobiopterin, and water. EC 1.14.16.2.
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
A mixed-function oxygenase that catalyzes the hydroxylation of a prolyl-glycyl containing peptide, usually in PROTOCOLLAGEN, to a hydroxyprolylglycyl-containing-peptide. The enzyme utilizes molecular OXYGEN with a concomitant oxidative decarboxylation of 2-oxoglutarate to SUCCINATE. The enzyme occurs as a tetramer of two alpha and two beta subunits. The beta subunit of procollagen-proline dioxygenase is identical to the enzyme PROTEIN DISULFIDE-ISOMERASES.
Chemical substances that are foreign to the biological system. They include naturally occurring compounds, drugs, environmental agents, carcinogens, insecticides, etc.
Cytoplasmic proteins that bind certain aryl hydrocarbons, translocate to the nucleus, and activate transcription of particular DNA segments. AH receptors are identified by their high-affinity binding to several carcinogenic or teratogenic environmental chemicals including polycyclic aromatic hydrocarbons found in cigarette smoke and smog, heterocyclic amines found in cooked foods, and halogenated hydrocarbons including dioxins and polychlorinated biphenyls. No endogenous ligand has been identified, but an unknown natural messenger with a role in cell differentiation and development is suspected.
A liver microsomal cytochrome P-450 monooxygenase capable of biotransforming xenobiotics such as polycyclic hydrocarbons and halogenated aromatic hydrocarbons into carcinogenic or mutagenic compounds. They have been found in mammals and fish. This enzyme, encoded by CYP1A1 gene, can be measured by using ethoxyresorufin as a substrate for the ethoxyresorufin O-deethylase activity.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
A chemical by-product that results from burning or incinerating chlorinated industrial chemicals and other hydrocarbons. This compound is considered an environmental toxin, and may pose reproductive, as well as, other health risks for animals and humans.
A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.
A plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. Bixa contains bixin. Cochlospermum contains arjunolic acid and gum kondagogu (POLYSACCHARIDES).
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
An inhibitor of the enzyme STEROID 11-BETA-MONOOXYGENASE. It is used as a test of the feedback hypothalamic-pituitary mechanism in the diagnosis of CUSHING SYNDROME.
A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Organic compounds containing a BENZENE ring attached to a flavone group. Some of these are potent arylhydrocarbon hydroxylase inhibitors. They may also inhibit the binding of NUCLEIC ACIDS to BENZOPYRENES and related compounds. The designation includes all isomers; the 7,8-isomer is most frequently encountered.
A carcinogen that is often used in experimental cancer studies.
Individuals licensed to practice medicine.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Persons whose profession is to give legal advice and assistance to clients and represent them in legal matters. (American Heritage Dictionary, 3d ed)
Promotion and protection of the rights of children; frequently through a legal process.
Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.
Attitudes of personnel toward their patients, other professionals, toward the medical care system, etc.
Bites inflicted by humans.

Inhibition of benzo(alpha)pyrene metabolism catalyzed by mouse and hamster lung microsomes. (1/54)

Induced and constitutive microsomal enzymes of mouse and hamster lungs catalyze both the hydroxylation of benzo(alpha)pyrene and reactions that lead to its irreversible binding to macromolecules. For mouse and hamster, the induced lung hydroxylases have Km values of 1.10 and 0.52 muM, respectively. The induced hydroxylases are strongly inhibited by 7,8-benzoflavone and are stimulated by cyclohexene oxide, an inhibitor of epoxide hydrase. Formation of the macromolecular product by the induced "binding" enzyme follows. Michaelis-Menten kinetics, except for substrate inhibition, and has Km values of 0.52 and 0.25 muM for lung microsomes from mouse and hamster, respectively. These reactions are also inhibited by 7,8-benzoflavone. The reaction catalyzed by the constitutive hydroxylase of mouse lungs is characterized by a brief lag period but proceeds in a linear fashion after the lag. The enzyme requires 60 muM benzo(alpha)pyrene to achieve maximum reaction velocity. Above this concentration, strong substrate inhibition is observed; accurate values for Vmax and Km cannot be derived. The constitutive hydroxylases are moderately inhibited by butylated hydroxytoluene, retinol, cyclohexene oxide, and 7,8-benzoflavone. The product of the constitutive "binding" enzyme is formed in a reaction that follows Michaelis-Menten kinetics. The Km value for enzymes from mouse and hamster lungs are 11.8 and 4.9 muM, respectively. Formation of this product is strongly inhibited by butylated hydroxytoluene and by retinol but not strongly by 7,8-benzoflavone or cyclohexene oxide. Since other evidence indicates that a constitutive enzyme may be involved in carcinogenesis by benzo(alpha)pyrene and since this reaction is inhibited by two known anticarcinogens, we suggest that it may be involved in this process.  (+info)

The atherogen 3-methylcholanthrene induces multiple DNA adducts in mouse aortic smooth muscle cells: role of cytochrome P4501B1. (2/54)

OBJECTIVE: 3-Methylcholanthrene (MC), a polycylic aromatic hydrocarbon, induces atherogenesis in mice fed an atherogenic diet. In this study, we tested the hypothesis that MC would induce DNA adducts in mouse aortic smooth muscle cells (SMCs) and that cytochrome P4501B1 (CYP1B1) plays an important role in the activation of MC to genotoxic intermediates. METHODS: Cultured SMCs were treated with MC or the vehicle dimethyl sulfoxide (DMSO), and DNA was isolated after 24 h. In some experiments, the cells were pre-treated with the CYP1B1 inhibitor 1-ethynylpyrene (EP) prior to exposure to MC. DNA adducts were determined by the 32P-postlabeling assay. Aryl hydrocarbon hydroxylase assay was measured by fluorimetry. RESULTS: MC induced formation of 12 DNA adducts that were not observed in DMSO-treated cells. DNA adduct formation was dose-dependent, with maximum response observed at 3 microM. Pre-treatment of cells with EP dramatically suppressed DNA adduct formation by MC. MC treatment caused induction of CYP1B1, but not CYP1A1. CONCLUSION: The induction of high levels of multiple DNA adducts in SMCs by MC suggests that SMCs have a functional enzymatic machinery capable of metabolically activating MC to genotoxic metabolites. The significant inhibition by EP of MC-induced DNA adduct formation indicated that CYP1B1 was the primary CYP enzyme responsible for formation of genotoxic metabolites that may play a role in the induction of atherosclerosis by MC.  (+info)

Development of an assay for aryl hydrocarbon (benzo(a)pyrene) hydroxylase in human peripheral blood monocytes. (3/54)

An assay has been developed and measurements of aryl hydrocarbon [benzo(a)pyrene] hydroxylase have been made in peripheral blood monocytes from a human population. Treatment with benz(a)anthracene in cell culture increased aryl hydrocarbon hydroxylase activity from 6.5 to 37-fold in monocytes from each of 25 apparently healthy donors. A weak correlation (r = 0.38) was observed between the induction ratios obtained with monocytes and lymphocytes from the same donors. Reproducibilities of the monocyte and lymphocyte assays were comparable. In monocytes, the measurement of basal and induced aryl hydrocarbon hydroxylases activity does not require pretreatment with mitogens as is the case with lymphocytes. Monocytes also exhibit a much wider range of induction ratios than do lymphocytes.  (+info)

Mechanism of dioxin action: Ah receptor-mediated increase in promoter accessibility in vivo. (4/54)

We have analyzed dioxin-inducible, Ah receptor-dependent changes in protein-DNA interactions at the CYP1A1 transcriptional promoter in intact mouse hepatoma cells. Our findings indicate that in uninduced cells, the promoter is inaccessible to its cognate binding proteins, which are known to be expressed constitutively. Dioxin induces, in Ah receptor-dependent fashion, an increase in promoter accessibility, which occurs rapidly and does not require ongoing transcription of the CYP1A1 gene. The change in promoter accessibility is not due to an altered pattern of cytosine methylation at the promoter; it probably reflects a 2,3,7,8-tetrachlorodibenzo-p-dioxin- induced change in the chromatin structure. These findings provide new insight into the mechanism of dioxin action and contribute to a better understanding of the regulation of inducible gene transcription in mammalian cells.  (+info)

Regulation of aryl hydrocarbon (benzo-(A)-pyrene) hydroxylase activity in mammalian cells. Induction of hydroxylase activity by N6,O2'-dibutyryl8 adenosine 3':5'-monophosphate and aminophylline. (5/54)

Treatment of hamster BHK cells with N6,O2'-dibutyryl adenosine 3':5'-monophosphate (Bt2cAMP), aminophylline, theophylline, or papaverine increased the level of aryl hydrocarbon (benzo(a)pyrene) hydrolxylase activity. The highese increase, 100-fold, was obtained with Bt2cAMP plus aminophylline or theophylline. N2,O2-Dibutyryl guanosine 3':5'-monophosphate gave a lower induction than Bt2cAMP. The level of hydroxylase activity started to decrease 6 hours after treatment with the inducer and was reduced to almost the uninduced level after 24 hours. Repeated addition of Bt2cAMP and aminophylline did not prevent this decrease. The hydroxylase can also be induced by treating cells with benz(a)anthracene, and the level of this induced activity was maintained for 24 hours. Aminophylline gave a 2- to 8-fold stimulation of the induction by benz(a)anthracene. The enzyme activity induced by Bt2cAMP, aminophylline, and benz(a)anthracene converted benzo(a)pyrene to similar alkali-extractable metabolities with a fluorescence spectra similar to that of 3-hydroxybenzo(a)pyrene. These induced enzyme activities also showed a similar heat stability. Induction by Bt2cAMP and aminophylline, like induction by benz(a)anthracene, required continued protein synthesis and only an initial period of RNA synthesis. Compared to the benz(a)anthracene-induced hydroxylase with a Km of 4.3 muM, the hydroxylase induced by Bt2cAMP and aminophylline showed a Km of 0.14 muM, and was 100-fold more sensitive to inhibition by 7,8-benzoflavone. Increasing the serum concentration in the culture medium stimulated the induction by aminophylline but did not stimulate induction by benz(a)anthracene. The results indicate that aryl hydrocaarbon (benzo(a)pyrene) hydroxylase can be induced by compounds that increase the level of adenosine 3':5'-monophosphate and that this induction and induced enzyme activity differs from that caused by benz(a)anthracene.  (+info)

Activation of 3':5'-cyclic AMP-dependent protein kinase and induction of ornithine decarboxylase as early events in induction of mixed-function oxygenases. (6/54)

The parenteral administration of a single dose of 3-methylcholanthrene to rats caused an increase in the liver of the concentration of 3', 5'-cAMP and of the activity of cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37). These events were followed by an increased activity of ornithine decarboxylase (L-ornithine carboxy-lase, EC 4.1.1.17), the enzyme that controls the biosynthesis of polyamines. Finally, the activity of benzo[a]pyrene hydroxylase, as well as the amount of cytochrome P-448, was increased. Similarly, after the administration of phenobarbital, there was first an increase in the cAMP concentration and in the activity of cAMP-dependent protein kinase, then the induction of ornithine decarboxylase, and finally, an enhanced activity of ethylmorphine N-demethylase and an increased content of cytochrome P-450. These data suggest that the drug-induced processes in liver that increase the activities of the oxidative, and presumably other, drug-metabolizing enzymes include the following sequence of events: (1) increase in cAMP concentration and/or activation of cAMP-dependent protein kinase; (2) induction of ornithine decarboxylase; and, (3) induction of drug-metabolizing enzymes.  (+info)

NADH-dependent aryl hydrocarbon hydroxylase in rat liver mitochondrial outer membrane. (7/54)

NADH-dependent 3,4-benzpyrene hydroxylase activity was detected in the purified mitochondrial outer membrane fraction from the livers of rats treated with 3-methylcholanthrene. The specific activity in the outer membrane fraction is nearly equal to that of microsomes, a level too high to be accounted for only by the microsomal contamination. On the other hand, the NADPH-dependent 3,4-benzpyrene hydroxylase activity in the outer membrane fraction is about 50% of that of microsomes. The ratio of the specific activity of NADPH- to NADH-dependent 3,4-benzpyrene hydroxylase in microsomal fraction was about 3.5, while that of the outer membrane fraction was about 1.5. Moreover, it was found that NADH-dependent 3,4-benzpyrene hydroxylase activity in mitochondrial outer membrane from control rat liver was cyanide-insensitive, while that in microsomes was cyanide-sensitive. These results suggest the presence in the mitochondrial outer membrane fraction of aryl hydrocarbon hydroxylase activity which uses as electron donor NADH nearly to the same extent as NADPH. The hydroxylase system is composed of cyanide-insensitive cytochrome P-450 and is inducible markedly by 3-methylcholanthrene treatment. The probable electron transfer pathways in the mitochondrial outer membrane cytochrome P-450 oxidase system are discussed.  (+info)

A relative deficiency of cytochrome P-450 and aryl hydrocarbon [benzo(a)pyrene] hydroxylase in hyperplastic nodules induced by 2-acetylaminofluorene in rat liver. (8/54)

The concentrations of cytochrome P-450 and the activities of aryl hydrocarbon [benzo(a)pyrene] hydroxylase (AHH) and reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase were measured in early (gray-white) and remodeled (brown) hyperplastic nodules induced in the livers of rats with 2-acetylaminofluorene and were compared to the values in control livers and in the liver surrounding the nodules. Cytochrome P-450 content of early (14 weeks) hyperplastic nodules is 30% of the activity of untreated control livers and 48% of the activity of the surrounding liver. AHH activity of the early nodules is 10% of the control activity and 33% of the activity in the surrounding nonnodular liver. Nicotinamide adenine dinucleotide phosphate-cytochrome c reductase activity in the microsomes of early nodules is 76% of the control activity and 78% of the activity in the surrounding liver. In the late remodeled nodules, (22 and 25 weeks), the cytochrome P-450 content is 40% of that of controls and AHH activity is 15% of the control activity. In primary hepatomas induced by 2-acetylaminofluorene, cytochrome P-450 content is 21% of that of controls, AHH activity is 11% of the activity of controls, and reductase is 50% of the control activity. These results, indicating a relative nodule deficiency in some of the cellular components believed to be important in the activation of hepatocarcinogens and hepatotoxins, offer one possible explanation for the relative resistance to carcinogen cytotoxicity of hyperplastic liver nodules.  (+info)

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4.1 Polymorphisms and mutations are both variations in DNA sequence and can arise through the same mechanisms. We use the term polymorphism to refer to DNA variants that are relatively common in populations. Mutations affect the phenotype.. 4.2 Misreading of bases during replication can lead to substitution and can be caused by things like tautomerism, DNA alkylating agents, and irradiation.. 4.3 Looping out of DNA on the template strand during replication; strand breakage, due to radiation and other mutagens; and (discussed in earlier chapters) chromosomal aberrations such as deletions and translocations.. 4.4 Looping out of DNA on the growing strand during replication; transposition; and (discussed in earlier chapters) chromosomal aberrations such as duplications, insertions, and translocation.. 4.5 Benzopyrene is one of many hazardous compounds present in smoke. Benzopyrene is an intercalating agent, which slides between the bases of the DNA molecule, distorting the shape of the double helix, ...
Trifluralin market on the basis of type, application, and geography. This report provides a quantitative analysis of the current trends and estimations to 2025
The present study shows the effect of combined dietary deprivation of fat and vitamin A on benzo(a)pyrene (BaP) induced lung carcinogenesis in male Wistar rats. Lung tumors were induced by intratracheal instillation of BaP-Fe2O3 in normal saline. The tumor incidence and tumor burden in control animals were 82% and 2.28 respectively. Fat deficiency decreased the tumor incidence to 57% and tumor burden to 1.66. On the other hand, in vitamin A deficiency these were 83% and 4.02 respectively. Fat deprivation in the diet of animals fed with vitamin A deficient diet decreased the tumor incidence and tumor burden to 69.6% and 2.7 respectively. The results suggest a protective role of low intake of fat in vitamin A deficiency for BaP-induced lung tumorigenesis in rats.
Classification is based on the induction of urinary tract tumors (renal pelvis carcinomas and urinary bladder papillomas) and thyroid tumors (adenomas/carcinomas combined) in one animal species (F344 rats) in one study. Trifluralin is structurally similar to ethalfluralin, a carcinogen in the rat ...
Words Ending In ene: acetylene,achene,advene,agene,akene,alkene,amene,aminobutene,amylene,anadyomene,anthracene,antigene,antivenene,arrasene,azobenzene,bene,benzanthracene,benzene,benzoapyrene,benzopyrene,benzpyrene,buckm
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site. ...
Hydrase definition, any of the class of enzymes that catalyze the addition of a water molecule into a compound without causing hydrolysis. See more.
Words starting with T (page 84): triffic, triffid, triffidian, triffids, triffidy, trifid, trifle, trifled, trifler, trifles, trifling, triflingly, triflingness, triflings, trifluoperazine, trifluralin, trifocal, trifold, trifoliate, trifoliated...
This enzyme belongs to the family of hydrolases, specifically those acting on ether bonds (ether hydrolases). The systematic name of this enzyme class is cis-stilbene-oxide hydrolase. Other names in common use include epoxide hydratase (ambiguous), microsomal epoxide hydratase (ambiguous), epoxide hydrase, microsomal epoxide hydrase, arene-oxide hydratase (ambiguous), benzo[a]pyrene-4,5-oxide hydratase, benzo(a)pyrene-4,5-epoxide hydratase, aryl epoxide hydrase (ambiguous), cis-epoxide hydrolase, and mEH. This enzyme participates in metabolism of xenobiotics by cytochrome p450 ...
2 major biochemical reactions that occur in the peroxisome is the mixed function oxidase and the other is a catalase but what 2 enzymes are involved in these reactions?...beta oxidation one of them ...
Trichloroepoxypropane: A potent epoxide hydrase and aryl hydrocarbon hydroxylase inhibitor. It enhances the tumor-initiating ability of certain carcinogens.
Effects of five organic solvent vehicles on benzo(a)pyrene- hydroxylase (BP-hydroxylase) activity and on the benzo(a)pyrene (50328) (BP) metabolite profile were studied in lung microsomes prepared from male New Zealand white rabbits. The production of 3- OH-benzo(a)pyrene (13345216) and 9-OH-benzo(a)pyrene (17573216) was used to evaluate the effects of dimethyl-sulfoxide (DMSO), acetone, methanol,
soil pollution; phenanthrenes; fertilizer application; nitrogen; bioremediation; soil amendments; alkaline soils; lacustrine soils; soil microorganisms; biosolids; biodegradation; phosphorus; saline soils; benzopyrenes; polluted soils; nitrogen fertilizers; ...
en] Cytochrome P-450-dependent monooxygenases are able to oxidize a large variety of endogenous and exogenous substrates. This paper describes the in vitro interaction between benzopyrene and steroids at the level of two rat liver monooxygenases: steroid-16 alpha-hydroxylase and aryl hydrocarbon hydroxylase (AHH). The results obtained suggest the following conclusions: (1) Steroid-16 alpha-hydroxylase is partially supported by a specific cytochrome P-450 form which is not inhibited in vitro by exogenous substrates. Steroid-16 alpha-hydroxylase is completely independent from cytochrome P1-450 (or P-448), as it is insensitive, in vitro, to alpha-naphthoflavone; (2) AHH is supported by two cytochrome P-450 forms: a specific form which is inducible by methylcholanthrene and inhibited in vitro by alpha-naphthoflavone, but is insensitive to metyrapone and steroids; and another less specific form which is inhibited by metyrapone and steroids in vitro ...
Download Free Full-Text of an article EFFECTS OF 3 - METHYL CHOLANTHEREN ON LIVER MICROSOMAL MIXED FUNCTION OXIDASE ACTIVITIES OF ACIPENSER PERSICUS
Brundel, dang, I dont like hearing that about 7, 8 benzoflavone . I was about to pick up several bottles of Dermacrine when they were back in stock.
We measured aryl hydrocarbon hydroxylase (AHH) in cultured human lymphocytes. A striking seasonal variation in AHH activity was observed with induced AHH activity levels from January through May measuring approximately 20% of the values during the remainder of the year. AHH inducibility was determined by comparing lymphocytes from the same person cultured with and without the inducer 3-methylcholanthrene. If measurements are limited to the summer and fall seasons when AHH activity is high, AHH inducibility is reproducible for most persons with repeat determinations on the same person averaging 11% from the mean. The values of AHH inducibility in 53 persons ranged from 0.9 to 5.0, but the distribution of values did not fall into three distinct, nonoverlapping classes as reported by others. We were not able to determine the distribution of AHH inducibility in lung cancer patients since lymphocytes from less than half of the patients tested could be successfully cultured.
Lee, S.-M. and Clemens, M. G. (1992), Effect of α-tocopherol on hepatic mixed function oxidases in hepatic ischemia/reperfusion. Hepatology, 15: 276-281. doi: 10.1002/hep.1840150217 ...
The rat and hamster exhibit about a 100-fold difference in sensitivity to the acute toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), with the hamster representing the most resistant species examined to date. The present study compared the induction and inhibition of hepatic TCDD metabolism in these species using suspensions of isolated hepatocytes. Purified 14C-TCDD or 3H-TCDD (2.2 microM) was incubated for 2-6 hr with hepatocytes isolated from untreated, TCDD-pretreated (5 micrograms/kg, ip), 3-methylcholanthrene-pretreated (3-MC, 50 mg/kg, ip, x 3 days), isosafrole-pretreated (ISO, 150 mg/kg, ip, x 3 days), or phenobarbital-pretreated (PB, 80 mg/kg, ip, x 3 days) rats and hamsters. Untreated rat and hamster hepatocytes metabolized TCDD at a similar rate (0.20 and 0.18 pmol/hr/mg, respectively). In both species, TCDD and 3-MC pretreatments elevated the rate of TCDD metabolism by 5-6-fold, while PB pretreatment had no effect. isosafrole modestly increased (1.8-2.5-fold) TCDD metabolism in ...
The lnfluence of mlcrosomal and nuclear aryl hydrocarbon hydroxylase (AHH) actlvlty on the covalent blndlng of [G·3H]benzo(a )pyrene to rat llver DNA was evaluated in viWJ. lnductlon of mlcrosomal AHH was obtalned alter phenobarbltal treatment (160% of control), whlch also lncreased DNA blndlng to 190%, but left the nuclear actlvlty unchanged. Nuclear AHH was lnduced wlth dleldrln (150%), and the blndlng was decreased to 75%, whereaa the mlcrosomal AHH was at control Ievei. The lncreaslng effect of mlcrosomal AHH lnductlon as weil as the decreaslng effect of nuclear AHH lnductlon on the blndlng was shown clearly when the data of the Individual rata were uaed to solve the equatlon Binding = e•(mlcroeomal AHH) + b•(nuclear AHH) + c Multiple linear regresslon analysls wlth the data from 10 anlmala reaulted ln positive valuea for a and c, a negative value for b, and a good multiple correlatlon coefflclent of r = 0.974. Pretreatment wlth 3-methylcholanthrene ln· duced mlcrosomal AHH to 380% of ...
September 2020. In This Edition Articles Fundamental Aspects - Analysis and Identification of Mezcal and Tequila Aromas by Ambient Ionisation MS, GC-MS, and GCxGC-MS - Analysis of Polynuclear Aromatic Hy.... ...
September 2020. In This Edition Articles Fundamental Aspects - Analysis and Identification of Mezcal and Tequila Aromas by Ambient Ionisation MS, GC-MS, and GCxGC-MS - Analysis of Polynuclear Aromatic Hy.... ...
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 21248-00-0(6-BROMOBENZO(a)PYRENE),please inquire us for 21248-00-0(6-BROMOBENZO(a)PYRENE).
Method of biolistic transformation was used for genetic improvement of commercial barley cultivars (Oksamitoviy, Vodogray and Getman). The plasmid pHLFTuBA was used for particle bombardment that consists of the hLF gene under the control of the barley glutelin B-1 promoter and a selectable marker gene, α-tubulin conferring resistance to trifluralin (dinitroanilinr herbicide). Preliminary screening of different trifluralin concentration range from 0,1 to 30 μM was tested for determination of effective selective agent concentration. Two transgenic barley line of genotype Oksamitiviy and transgenic callus line of cultivar Getman were obtained after selection on 10 μM of trifluralin. To confirm the transgenic nature of regenerated plants, the PCR analysis was carried out. The 734bp length fragment of hLF gene was amplified from both regenerated plants.. ...
GC Application #16187: Polynuclear Aromatic Hydrocarbons (PAHs): US and EU Analytes on ZB-MR1. Column used: Zebron™ ZB-MultiResidue™-1, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea Part#: 7HG-G016-11
GC Application #15560: Polynuclear Aromatic Hydrocarbons (PAHs) on ZB-5MS. Column used: Zebron™ ZB-5MS, GC Cap. Column 20 m x 0.18 mm x 0.18 µm, Ea Part#: 7FD-G010-08
Concomitant exposures to arsenic and polycyclic aromatic hydrocarbons (PAHs) such as benzo[a]pyrene (BaP) are widespread. While BaP acts by binding to and inducing mutations in critical sites on DNA, the mechanism(s) of arsenic carcinogenesis remains unknown. Data from epidemiological studies of arsenic copper smelter workers and arsenic ingestion in drinking water suggest a positive interaction f
Chan, J T. and Black, H S., Skin carcinogenesis. Cholesterol-5alpha, 6alpha-epoxide hydrase activity in mouse skin irradiated with ultraviolet light. (1974). Subject Strain Bibliography 1974. 1966 ...
US EPA PC Code ); 001 (PDP Code); 045101 (US EPA PC Code Text ); 1,2,3,4,10,10-Hexachloro-1,4,4a,5,8,8a-hexahydro-(1a,4a,4ab,5a,8a,8ab)-1,4:5,8-dimethanonaphthalene; 1,2,3,4,10,10-Hexachloro-1,4,4a,5,8,8a-hexahydro-endo-1,4-exo-5,8-dimethanonaphthalene; 1,4:5,8-Dimethanonaphthalene, 1,2,3,4,10,10-hexachloro-1,4,4a,5,8,8a-hexahydro-, (1.alpha.,4.alpha.,4a.beta.,5.alpha.,8.alpha.,8; 309-00-2 (CAS number); 309002; 309002 (CAS number without hyphens); 9 (CA DPR Chem Code) ); Aldocit; Aldrex; Aldrin; ALDRIN (CA DPR Chem Code Text ); Aldrin [1,4:5,8-Dimethanonaphthalene, 1,2,3,4,10,10-hexachloro-1,4,4a,5,8,8a-hexahydro-(1.alpha.,4.alpha.,4a.beta.,5.alpha.,8.al; Aldrin/Dieldrin; Aldrine; Compound 118; Drinox; Eldrin; ENT-15949; Hexachlorohexahydro-endo, exo-dimethanonaphthalene; HHDM; HHDN; HHPN; Kortofin; Octalene; OMS 194; OMS-194; SD 2794; Seedrin; Tatuzinho; ...
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Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution ... Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution ... Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution ... Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution ...
Benzopyrene Hydroxylase / metabolism * Benzopyrenes / metabolism* * Coumarins / metabolism * Female * Hexobarbital / metabolism ...
Substrate-induced synthesis and other properties of benzopyrene hydroxylase in rat liver. J Biol Chem 1957;228:753-66. ...
Alpha-naphthoflavone inhibits the metabolism of 3,4-benzopyrene and 7,12-dimethylbenz(a)anthracene in hamster enlbryo cell ... Alpha-Naphthoflavone: An Inhibitor of Hydrocarbon Cytotoxicity and Microsomal Hydroxylase Message Subject. (Your Name) has ... Alphla-nalphthoflavone also inhibits the aryl hydrocarbon hydroxylase activity in homogenates of induced hamster embryo cells ...
Benzopyrene Hydroxylase. 1. Benzopyrenes. 2. Beta Rhythm. 1. Bicarbonates. 3. Bile. 1. Bile Duct Diseases. ...
Best, L. M., Leung, J., Freeman, S. C., Sutton, A. J., Cooper, N. J., Milne, E. J., Cowlin, M., Payne, A., Walshaw, D., Thorburn, D., Pavlov, C. S., Davidson, B. R., Tsochatzis, E., Williams, N. R. & Gurusamy, K. S., 16 Jan 2020, In : The Cochrane Library. 2020, 1, 94 p., CD013203.. Research output: Contribution to journal › Review article ...
High levels of benzopyrene hydroxylase and folate receptors: benzopyrene, a carcinogen contained in various foods, is converted ... including the benzopyrene hydroxylase, that may protect them against food-derived carcinogens (Delaunoit 2005). ... to less toxic metabolites by the enzyme benzopyrene hydroxylase, which is present in much higher concentrations in the small ...
Identification of the human liver cytochrome P-450 guilty quest of coumarin 7-hydroxy-lase labour. Intrarenal fluoride ... An updated inspect of the clinical unfolding of coumarin (1,2-benzopyrene) and 7-hydroxycoumarin. Phenotypic and genotypic ... Immunochemical and catalytical studies on hepatic coumarin 7-hydroxylase in mankind, rat, and mouse. ...
Methods 7 ethoxyresorufin O deethylase (EROD) and benzopyrene hydroxylase (ANH) activity were determined by ultraviolet ...
Animals, Aryl Hydrocarbon Hydroxylases, metabolism, Benzo(a)pyrene, toxicity, Benzopyrenes, Carcinogens, Cell Transformation, ...
Enhanced aggressiveness of benzopyrene-induced squamous carcinomas in transgenic mice overexpressing the proprotein convertase ... PACE4 (PCSK6). Combined participation of hydroxylase active site residues and effector protein binding in a para to ortho ...
Hydroxylases/aryl ; Tritium release assay ; Benzopyrenes ... Improved Method for the Determination of Aryl-4-hydroxylase ...
... steroid-16 alpha-hydroxylase and aryl hydrocarbon hydroxylase (AHH). The results obtained suggest the following conclusions: (1 ... This paper describes the in vitro interaction between benzopyrene and steroids at the level of two rat liver monooxygenases: ... Steroid-16 alpha-hydroxylase is completely independent from cytochrome P1-450 (or P-448), as it is insensitive, in vitro, to ... Steroid-16 alpha-hydroxylase is partially supported by a specific cytochrome P-450 form which is not inhibited in vitro by ...
They cant spell and seem to miss the point that 7,8 benzo is very different from benzopyrenes. October 8, 2009 2:09 PM ... Aryl Hydrocarbon Hydroxylase- and Polycyclic Hydrocarbon Tumorigenesis: Effect of the Enzyme Inhibitor 7,8-Benzoflavone on ... Benzopyrene - Wikipedia, the free encyclopedia. Proc. Nat. Acad. Sci. Vol. 69, No. 4, pp. 824-828, April 1972. ... 1. Hydrocarbon Hydroxylase- and Polycyclic Hydrocarbon Tumorigenesis: Effect of the Enzyme Inhibitor 7,8-Benzoflavone on ...
Benzopyrene Hydroxylase D8.244.453.40.110 D8.244.453.05.110 D8.811.682.690.708.170.40.110 D8.811.682.690.708.170.10.110 D12.776 ... Aniline Hydroxylase D8.244.453.40.50 D8.244.453.05.50 D8.811.682.690.708.170.40.50 D8.811.682.690.708.170.10.50 D12.776.422.220 ... Vitamin D3 24-Hydroxylase D8.244.453.978 D8.244.453.496.500 D8.811.682.690.708.170.957 D8.811.682.690.708.170.469.500 D12.776. ... Aryl Hydrocarbon Hydroxylases D8.244.453.40 D8.244.453.05 D8.811.682.690.708.170.40 D8.811.682.690.708.170.10 D12.776.422.220. ...
... benzopyrene hydroxylase, aminopyrene-n-demethylase, aniline hydroxylase except NADH cytochrome e reductase which increased ...
Polycarbon aromatics: e.g., benzopyrene in smoke and cooked meat. • Aflatoxin- fungus on peanuts and other foods- not so much ... Tyrosine hydroxylase inhibitor used for multiple types of cancers such as myelogenous leukemia. ...
Dopamine beta hydroxylase Found in effluent ff adrenergic nerve stimulation Dosing rate Clearance x target conc Durham-Humprey ... Benzopyrene, Phenobarbital, phenytoin, rifampin Epinephrine Treat acute hypersensitivity rxn to drugs Esmolol In OR develop arr ...
Conversely, toxaphene decreased the incidence of BP-induced tumors and inhibited BP hydroxylase activity in the lungs of the A/ ... Benzopyrene ; Oncogenesis ; Phosphorothiotic acid/O-O-(dimethyl-ester)-O-(nitrophenyl-ester) ... These results suggest that increased BP hydroxylase activity in tissues tends to enhance tumor formation and a decrease in the ... On the other hand, toxaphene enhanced benzo(a)pyrene (BP)-induced tumors and increased BP hydroxylase activity in the ...
Animals, Aryl Hydrocarbon Hydroxylases, Benzopyrenes, Carcinogens, Cytochrome P-450 CYP1B1, Female, Male, Mice, Models, ...
I. Effect of antler and old antler on liver injury induced by benzopyrene in rats. Proc. Japanese Soc. Food & Nutrition 23: 9. ... Nerve fibres immunoreactive to neuropeptide Y, C-flanking peptide of neuropeptide Y and tyrosine hydroxylase, all present in ...
Molecular phenotyping of transient postnatal tyrosine hydroxylase neurons in the rat bed nucleus of the stria terminalis. ... Genome-wide H3K9 histone acetylation profiles are altered in benzopyrene-treated MCF7 breast cancer cells. Journal of ... Molecular phenotyping of transient postnatal tyrosine hydroxylase neurons in the rat bed nucleus of the stria terminalis. ... Genome-wide H3K9 histone acetylation profiles are altered in benzopyrene-treated MCF7 breast cancer cells. Journal of ...
... like benzopyrene) Radiation X-rays create free radicals UV creates thymine dimers Artificial or Natural mutations HOT SPOTS FOR ... abnormal enzyme phenylalanine hydroxylase normally catalyzes conversion of dietary phenylalanine to tyrosine. Has tryptophan ...
phenylalanine hydroxylase products*ADH1A products*CYP1B1 products*ADH6 products*ADH4 products*ADH1C products*ADH1B products* ... benzopyrenes*aromatic polycyclic hydrocarbons*quinones*carcinogens*dna adducts*molecular structure*reactive oxygen species* ... aryl hydrocarbon hydroxylases*tumor cell line*isomerism*dna*catalysis*aldehyde reductase*indicators and reagents*g1 phase*s ...
4-benzopyrene/angiotensin II-activated macrophage in abdominal aortic aneurysm. Stem Cells Int. 2017, 9495739. ... Suppression of abdominal aortic aneurysm formation by inhibition of prolyl hydroxylase domain protein through attenuation of ...
Polycyclic Aromatic Hydrocarbons (PAH): e.g. benzopyrene produces p53 mutation (G to T transversion) ... Identified gene: CYP1A1 (of Cytochrome P-450 family) - codes for aryl hydrocarbon hydroxylase ...
... aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N.. ...
Hydroxylase, Aniline. Hydroxylase, Benzopyrene. Hydroxylase, Butyrobetaine. hydroxylase, cinnamate 4-. Hydroxylase, Collagen ...
  • Fish from petroleum-contaminated sites in the marine environment have elevated levels of benzopyrene hydroxylase activity in liver and gill tissue. (sciencemag.org)
  • Alpha-naphthoflavone inhibits the metabolism of 3,4-benzopyrene and 7,12-dimethylbenz(a)anthracene in hamster enlbryo cell cultures and protects the cells against the inhibition of cell multiplication by these carcinogens. (sciencemag.org)
  • The present study examines the time course of appearance of tyrosine hydroxylase (TH) immunoreactivity in the principal neurons and SIF cells of the rat sphenopalatine ganglion. (faintpower.gq)
  • Aryl Hydrocarbon Hydroxylase- and Polycyclic Hydrocarbon Tumorigenesis: Effect of the Enzyme Inhibitor 7,8-Benzoflavone on Tumorigenesis and Macromolecule Binding. (worldclassbodybuilding.com)
  • These results suggest that increased BP hydroxylase activity in tissues tends to enhance tumor formation and a decrease in the enzyme activity may have a protective effect agains tumors. (epa.gov)
  • en] Cytochrome P-450-dependent monooxygenases are able to oxidize a large variety of endogenous and exogenous substrates. (uliege.be)
  • The results obtained suggest the following conclusions: (1) Steroid-16 alpha-hydroxylase is partially supported by a specific cytochrome P-450 form which is not inhibited in vitro by exogenous substrates. (uliege.be)
  • On stipulated day the animals were slaughtered, their livers taken out and microsomes prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N. (thefreedictionary.com)
  • Alphla-nalphthoflavone also inhibits the aryl hydrocarbon hydroxylase activity in homogenates of induced hamster embryo cells and in liver microsomes from rats previously treated with polycyclic aromatic hydrocarbons, but not in microsomes from control rats. (sciencemag.org)
  • This paper describes the in vitro interaction between benzopyrene and steroids at the level of two rat liver monooxygenases: steroid-16 alpha-hydroxylase and aryl hydrocarbon hydroxylase (AHH). (uliege.be)
  • On the other hand, toxaphene enhanced benzo(a)pyrene (BP)-induced tumors and increased BP hydroxylase activity in the forestomach of the Ha/ICR mice and carbaryl enhanced BP-induced tumors and increased BP hydroxylase activity in the lungs of the A/J mice. (epa.gov)
  • Conversely, toxaphene decreased the incidence of BP-induced tumors and inhibited BP hydroxylase activity in the lungs of the A/J mice. (epa.gov)