Phagocytosis and killing of Mycobacterium avium complex by human neutrophils. (1/36)

Organisms belonging to the Mycobacterium avium complex (MAC) cause life-threatening bacteremia in immunocompromised patients. Monocytes and macrophages are thought to be responsible for ingestion and killing of MAC. However, it has been suggested that neutrophils may play a role in the early immune response to MAC infection. Here, neutrophils in autologous plasma were incubated (at 0 and 37 degrees C) with M. avium labeled with Auramine O, a potent fluorochrome. Neutrophil phagocytosis was measured by flow cytometry. Neutrophils incubated at 37 degrees C showed an increase in fluorescence over time with a maximum at 15 min, whereas neutrophils on ice showed no time-dependent increase in FL1. At 15 min Fl 1 at 37 degrees C was twice as high as FL1 at 0 degrees C. Examination under the fluorescent microscope showed multiple intracellular fluorescent mycobacteria. Results in nine independent experiments showed time-dependent decrease of colony-forming units in neutrophil-associated live M. avium. Significant killing was observed within 30 min and was complete by 120 min. Observation by electron microscopy clearly confirmed the presence of intraphagosomal MAC, both intact and with evidence of degradation. These data demonstrate that MAC is rapidly phagocytized and killed by human neutrophils. The newly established flow cytometry method should be useful in further studies of neutrophil function and of the role of G-CSF and other cytokines in MAC disease.  (+info)

Lessons from a proficiency testing event for acid-fast microscopy. (2/36)

OBJECTIVES: To evaluate the routine performance and the technical parameters of different acid-fast staining methods: Kinyoun, Ziehl-Neelsen (ZN), auramine, and auramine-rhodamine. DESIGN AND PARTICIPANTS: The performance of 167 laboratories was analyzed using prestained and unstained slides. SETTING: Laboratories holding New York State permits. RESULTS: The results revealed that Kinyoun's cold carbol fuchsin method is inferior to both the ZN and fluorochrome (auramine and/or auramine-rhodamine) methods. Even though 91% of the participants used commercial staining kits, the study identified unexpected errors concerning the concentration of carbol fuchsin, time for staining and counterstaining, and the concentration of acid alcohol for decolorization, which may significantly influence the sensitivity. Besides these findings, the present study showed that the examination of < 300 view fields may also decrease the sensitivity of acid-fast microscopy. In addition, we found that the sensitivity and specificity of the ZN and fluorochrome methods are comparable if the procedural standards are followed. CONCLUSIONS: The strict and ongoing quality control of the "simple to perform" acid-fast microscopy and the immediate review of commercially available staining kits are necessary. Because of the rapidity of the fluorochrome method, laboratories with large specimen numbers should use this technique. In all other cases, the ZN method should be used. Moreover, all clinicians should be aware of the method of acid-fast microscopy used and the proficiency of the laboratory in performing the assay.  (+info)

Interactions of heteroaromatic compounds with nucleic acids. A - T-specific non-intercalating DNA ligands. (3/36)

In the present paper we report the results of a study on the base specificity and affinity of eight dyes potentially able to interact with DNA. These compounds include four triphenylmethane dyes used in histochemistry, auramine, "Hoechst 33258" and two acridines substituted with t-butyl groups. They were selected with regard to their inability to intercalate between the base pairs of helical polynucleotides due to structural limitations. Hydrodynamic studies performed with the DNA complexes of crystal violet and Hoechst 33258 confirmed our assumptions that compounds of this type bind to the outside of DNA. The main results from DNA binding studies indicate that the triphenylmethane dyes except p-fuchsin are bound with high preference to two adjacent A - T pairs while Hoechst 33258 seems to need three A - T pairs as the binding site. Model studies with synthetic polynucleotides revealed that not only a sequence of A - T pairs, but also their structural arrangement in a helix, is crucial for the high affinities observed for most of the ligands when interacting with natural DNA. Methyl green and Hoechst 33258 can be used for increasing the resolution power of cesium chloride density gradients for DNAs with different (A + T) content.  (+info)

Optimisation of acid fast smears for the direct detection of mycobacteria in clinical samples. (4/36)

AIMS: Despite its long history, the acid fast smear remains unstandardised. Technical variations in both the preparation of clinical material and subsequent staining mean that smear sensitivity relative to culture may vary from 50% to over 80%. This study assessed the sensitivity of acid fast microscopy at each of five stages of sample preparation and by both commonly used staining methods. METHODS: Sputum samples thought for varying reasons to be highly likely to be culture positive were used to prepare a series of smears in which the effects of digestion (liquefaction), concentration (centrifugation), and decontamination (sodium hydroxide) could be assessed, together with a comparison of staining by the auramine/phenol and Ziehl-Neelsen techniques. RESULTS: The most effective method for the demonstration of acid fast organisms in sputum was found to be an auramine phenol stain applied to a liquefied, concentrated sample and examined before the decontamination process. CONCLUSIONS: The auramine phenol stain applied to a liquefied, concentrated sample and examined before the decontamination process is the most effective method for the demonstration of acid fast organisms in sputum.  (+info)

Direct detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis in auramine-rhodamine-positive sputum specimens by real-time PCR. (5/36)

Our objective was to evaluate the feasibility of a molecular assay based on a real-time PCR technique, carried out with a LightCycler instrument (Roche Biochemicals), to identify Mycobacterium tuberculosis bacilli and to detect rifampin and isoniazid resistance in DNA extracts from sputum samples. We studied three genes: rpoB, which is associated with rifampin resistance, and katG and inhA, which are associated with isoniazid resistance. A total of 205 sputum samples collected from 108 patients diagnosed with pulmonary tuberculosis with positive auramine-rhodamine-staining (AR) sputum samples, were tested. The sensitivities of the LightCycler PCR assay for the positive AR specimens was 97.5% (200 of 205) for rpoB and inhA genes and 96.5% (198 of 205) for the katG gene. For the total number of patients tested, the sensitivity was 100% (108 of 108 patients) for rifampin, whereas the sensitivity was 98.1% (106 of 108 patients) for isoniazid. Full agreement was found with the Bactec MGIT 960 method and the genotype inferred from the LightCycler data for rifampin. The phenotypic method for isoniazid reported 13 resistant strains (> or = 0.1 microg/ml). In seven (53.8%) strains there was a concordance between both methods, but we found that six (46.2%) strains reported as resistant by the phenotypic method were determined to be susceptible by real-time PCR. For the 75 strains reported as susceptible by the phenotypic method, the concordance with the LightCycler data was 100%. Our results demonstrate that rifampin-resistant M. tuberculosis could be detected in DNA extracted from auramine-rhodamine-positive sputum samples in a single-tube assay that took less than 3 h to perform for a collection of auramine-rhodamine-positive specimens obtained from patients with culture-documented pulmonary tuberculosis. Similarly, this occurs in half of the isoniazid-resistant M. tuberculosis DNA extracted from auramine-rhodamine-positive specimens.  (+info)

Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens. (6/36)

The performances of the BDProbeTec ET (Becton Dickinson) and COBAS AMPLICOR MTB (Roche) were retrospectively evaluated for detecting Mycobacterium tuberculosis complex in various respiratory specimens. The BACTEC and MGIT liquid culture system (Becton Dickinson) was used as a reference method. A total of 824 respiratory specimens, comprised of sputa, bronchoalveolar lavage fluid, and bronchial and tracheal aspirates from 580 patients, were evaluated. Out of 824 clinical specimens, 109 specimens from 43 patients were culture positive for M. tuberculosis. Of these 109 specimens, 67 were smear positive, 85 were positive by the COBAS AMPLICOR MTB test, and 94 were positive by the BDProbeTec ET. Of the 715 culture-negative specimens, 17 were positive by the auramine staining, 11 were positive by the COBAS AMPLICOR MTB test, and 12 were positive by the BDProbeTec ET. After discrepancy analysis and review of the patients' clinical data, 130 specimens from 50 patients were considered "true-positive" specimens. This resulted in the following sensitivities: microscopy, 61.5%; COBAS AMPLICOR MTB test, 78.0%; and BDProbeTec ET, 86.2%. The specificities of each system, based on the clinical diagnosis, were 99.7% for microscopy, 99.9% for the COBAS AMPLICOR MTB test, and 99.9% for the BDProbeTec ET. The data presented represent a considerable number of specimens evaluated with a considerable number of culture- and auramine-positive and culture-positive and auramine-negative results and therefore give a realistic view of how the data should be interpreted in a daily routine situation. Specifically, the data with regard to the culture-positive and auramine-negative specimens are useful, because in a routine situation, auramine-negative specimens are sometimes accepted, on clinical indications, to be analyzed by an amplification method.  (+info)

Improved sensitivity of sputum smear microscopy after processing specimens with C18-carboxypropylbetaine to detect acid-fast bacilli: a study of United States-bound immigrants from Vietnam. (7/36)

The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C18-carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remaining sputum was split; half was cultured, and the other half was incubated with CB-18 for 24 h, centrifuged, and examined for AFB by both staining methods. CB-18 processing improved the sensitivity of AFB staining by 20 to 30% (only differences in auramine sensitivity were statistically significant) but reduced specificity by approximately 20% (P < 0.05). These findings have direct utility for overseas migrant tuberculosis screening programs, for which maximizing test sensitivity is a major objective.  (+info)

Comparison of the sodium hydroxide specimen processing method with the C18-carboxypropylbetaine specimen processing method using independent specimens with auramine smear, the MB/BacT liquid culture system, and the COBAS AMPLICOR MTB test. (8/36)

A study was performed to diagnose tuberculosis by smear, culture, and nucleic acid amplification. The study was comprised of two independent arms. Each arm used a different specimen processing method; in one arm, all specimens were processed with N-acetyl-l-cysteine-sodium hydroxide, and in the other arm, all specimens were processed with C(18)-carboxypropylbetaine and lytic enzymes. In each arm, all processed sediments were split for analysis by auramine smear, by culture using the MB/BacT liquid culture system and solid media, and by nucleic acid amplification using the COBAS AMPLICOR MTB test. In the N-acetyl-l-cysteine-sodium hydroxide arm, 1,468 specimens were analyzed: 65 were smear positive; 88 and 42 were culture positive for Mycobacterium tuberculosis and nontuberculous mycobacteria, respectively; and 103 were PCR positive. Relative to cultures positive for M. tuberculosis, the sensitivity and specificity of the smear were 68.2% and 99.6%, respectively, and those of PCR were 75.0% and 97.3%, respectively. In the C(18)-carboxypropylbetaine study arm, 1,423 specimens were analyzed: 44 were smear positive; 82 and 31 were culture positive for M. tuberculosis and nontuberculous mycobacteria, respectively; and 91 were PCR positive. The sensitivity and specificity of the smear were 48.8% and 99.7%, respectively, and those of PCR were 78.0% and 98.0%, respectively. When the two arms were compared, C(18)-carboxypropylbetaine specimen processing significantly increased the number of smear-negative and culture-positive specimens and significantly increased the PCR sensitivity among this same group of specimens while at the same time significantly reducing the inhibition rate.  (+info)

China Basic Dye Basic Yellow 2 Auramine O, Find details about China Basic Yellow 2, Basic Dye from Basic Dye Basic Yellow 2 Auramine O - Hangzhou Emperor Chemical Co., Ltd.
This image was produced using fluorescence microscopy, staining the cells with compounds that bind to the cell walls and fluoresce. The blue cells have walls made of cellulose and their blue fluorescence is due to the calcofluor that theyve been stained with, which fluoresces blue in ultraviolet light. Calcofluor has been used as a blue whitener in washing powders - it binds to the cellulose in cotton fabrics and fluoresces faintly blue in the UV component of sunlight. The yellow staining is due to another fluorescent dye (fluorochrome) called auramine O, which binds to cutin in the outer cuticle of the plant, and to dead, lignified cell walls that give the stem its strength - and it fluoresces yellow. The cuticle in this cross section is the thin yellow line covering the outer surface of the section. The yellow circle in the centre is composed of dead, lignified cells - not particularly well developed in goosegrass because it scrambles over surrounding vegetation rather then investing ...
In this image one of the nodules has been cut in transverse section and stained with the fluorochromes calcofluor and auramine O. The plant root, with its xylem vessels visible, is at the top﻽. The bacteria filling the root nodule, encased in blue-stained plant cells, are stained yellow. The Rhizobium bacteria in the soil penetrate through a root hair, trigger proliferation of the host plant root cells to form a nodule and multiply within. Healthy root nodules are pink when you cut them open due to the presence of leghaemoglobin which, like haemoglobin in mammalian blood, absorbs oxygen. This is important because oxygen would otherwise inhibit the enzymes in the nodule that fix nitrogen into soluble forms. Bacterial nodules that are not pink when you cut them open are likely to be parasitic on the host plant, rather than symbiotic. ...
Direct visual examinations of Lower respiratory specimens: KOH preparation or periodic acid-schiff-stained smears for fungal element detection, Gram staining (for bacteria and yeasts), Ziehl Neelsen staining for Mycobacterium tuberculosis (or Kinyoun carbolfuchsin stain, Auramine or auramine-rhodamine) is commonly used direct examination methods for direct examination of lower respiratory specimens depending on the clinical presentation or as per the request of physician. ...
Spot diagnoses are (microscopic) diagnoses one can make on the spot. They are ideal for bell-ringer type exams. There is a separate article for gross spot diagnoses. If you are looking for questions for review/practice, take a look at the quizzes page for quick ones, and cases page for longer ones. ...
In article ,3vtpbj$gj5$1 at mhadg.production.compuserve.com,, ,74643.642 at CompuServe.COM, writes: , Path: interramp.com!psinntp!psinntp!psinntp!rutgers!uwm.edu!psuvax1!gvls1!newsfeed.pi tt.edu!godot.cc.duq.edu!news.duke.edu!solaris.cc.vt.edu!news.bluesky.net!news.s printlink.net!newsfeed.internetmci.com!news.compuserve.com!news.production.comp userve.com!news , From: Kevin Forward ,74643.642 at CompuServe.COM, , Newsgroups: bionet.microbiology , Subject: Re: Acid Fast Stains: Gastric Asp. , Date: 4 Aug 1995 18:36:35 GMT , Organization: CompuServe, Inc. (1-800-689-0736) , Lines: 7 , Message-ID: ,3vtpbj$gj5$1 at mhadg.production.compuserve.com, , References: ,3tfc0h$1nt2 at usenetw1.news.prodigy.com, , , I think the suggestion that this is a reasonable procedure is , incorrect. Saprophytic AFB are fairly common in the stomach and , since the true positives are rare in most settings the predictive , value of an AFB smear on gastric aspirates has a very low , predictive value. , , K. Forward As I ...
Eventbrite - Maximiliano Gutierrez presents The Acid Fast Club Winter 2018 - Monday, 15 January 2018 at Francis Crick Institute, London, England. Find event and ticket information.
Detection of acid-fast bacilli (AFB) in stained smears examined microscopically may provide the first bacteriologic clue of TB. Fluorochrome staining with auramine-rhodamine is the preferred staining method because it is faster than the traditional methods in which Ziehl-Neelsen or Kinyoun (basic fuchsin dye) stains are used. Smear examination is an easy and quick procedure; results should be available within 24 hours of specimen collection. However, smear examination permits only the presumptive diagnosis of TB because the AFB in a smear may be mycobacteria other than M. tuberculosis. Furthermore, many TB patients have negative AFB smears.. Positive cultures for M. tuberculosis confirm the diagnosis of TB; however, TB may also be diagnosed on the basis of clinical signs and symptoms in the absence of a positive culture. Culture examinations should be done on all specimens, regardless of AFB smear results. The BACTEC Radiometric System or other recently developed liquid medium systems allow ...
Acid Fast Yellow G 6359-98-4 NMR spectrum, Acid Fast Yellow G H-NMR spectral analysis, Acid Fast Yellow G C-NMR spectral analysis ect.
A method and apparatus for collecting and separating cells, particles or the like from body fluid or the like for microscopic diagnosis is disclosed. Fluid is sucked by capillary action through a fluid-permeable surface (17) which holds back the cell material to be investigated. An apparatus suitable for this purpose comprises a filter container (2) having an upper and a lower opening, the lower opening of which is covered by a fluid-permeable cell carrier element (10), at least one filter segment (11) and a suction insert (12) being arranged in the filter container (2).
For any reuse or distribution, you must make clear to others the license terms of this work. The best way to do this is with a link to this web page: http://creativecommons.org/licenses/by/2.0 ...
The Great Lakes Water Quality Agreement, in which the US and Canada agreed to restore and preserve the biolgoical, physical and chemical integrity of the Great Lakes Basin Ecosystem, was first signed in 1972. In 1987, a Protocol was signed by both governments which defined Great Lakes Areas of Concern (AOC) as "geographic areas that fail to meet the general or specific objectives of the agreement where such failure has caused or is likely to cause impairment or beneficial use of the areas ability to support aquatic life." The U.S. and Canadian governments identified 43 such areas; 26 in the U.S., 12 in Canada and five shared between the U.S. and Canada on connecting river systems. The Great Lakes Water Quality Agreement, as amended via the 1987 protocol, directs the two federal governments to cooperate with state and provincial governments to develop and implement Remedial Action Plans (RAPs) for each AOC. The Protocol also called for reports on restorative progress and for the International ...
The Ziehl-Neelsen stain, also known as the acid-fast stain, was first described by two German doctors; Franz Ziehl (1859 to 1926), a bacteriologist and Friedrich Neelsen (1854 to 1894), a pathologist [1].It is a special bacteriological stain used to identify acid-fast Mycobacteria. The preparation of Ziehl-Neelsen slides require several procedures and the slide should be examined under oil immersion by using the 100X magnification. There are some factors that may degrade the image quality such as under expose, over expose, under stain and thick samples. Thus, the images should be enhanced to improve the image quality in term of contrast and intensity. This paper proposes a contrast enhancement technique based on bright stretching and dark stretching algorithms for color images. Although the adopted image processing technique is quite simple, the results indicate that our method may have some potential to be used for improving the quality of Ziehl-Neelsen slide images ...
A 70-month analysis of Gram-stained smears at the laboratory of the Denver Metro Health Clinic suggests that the current standard cutoff for the microscopic diagnosis of male urethritis should be lowered from =5 to =2 polymorphonuclear cells/high power field ...
Certification Page. Declaration. Dedication. Acknowledgement. Tables of contents. List of Tables. Abstract. CHAPTER ONE. INTRODUCTION. 1.1 Background to the Study. 1.2 Statement of the Problem. 1.3 Research Questions. 1.4 Research Objectives. 1.5 Significance of the Study. 1.6 Scope and Limitations of the study. 1.7 Chapterisation. CHAPTER TWO. 2.1 Conceptual literature. 2.1.1 Malaria. 2.1.2 Signs and symptoms. 2.1.3 Complications. 2.1.4 Diagnosis of malaria. 2.1.5 Microscopic diagnosis. 2.1.6 Antigen Detection. 2.1.7 Serology. 2.1.8 Economic burden. 2.2 Empirical framework. CHAPTER THREE. RESEARCH MEDTHODOLOGY. 3.1 introduction. 3.2 Study area. 3.3 Sources and methods of data collection. 3.4 Population. 3.5 data Analysis. 3.5.1 Production function model. CHAPTER FOUR. 4.1 Introduction. 4.2 Presentation and analysis of data. 4.3 Testing the validity of the model. CHAPTER FIVE. 5.1 Introduction. 5.2 Summary. 5.3 Conclusions. 5.4 Recommendation. References. CHAPTER ONE 1.1 Background of the ...
Mycobacterium smegmatis is 3.0 to 5.0 µm long with a bacillus shape, an acid-fast bacterial species in the phylum Actinobacteria. It can be stained by Ziehl-Neelsen method and the auramine-rhodamine fluorescent method. It was first reported in 1884. Alvarez and Tavel found organisms similar to Lustgarten, who first discovered Mycobacterium. This organism was later named M. smegmatis. It is considered a non-pathogenic microorganism although, in rare cases, it can cause disease. M. smegmatis is useful for the research analysis of other Mycobacteria species in laboratory experiments. Since it is a fast grower and non-pathogenic it makes a simple model that is easy to work with. It shares more than 2000 homologs with M. tuberculosis and shares the same unusual cell wall structure of M. tuberculosis and other mycobacterial species. The discovery of plasmids, phages, and mobile elements has enabled the construction of dedicated gene-inactivation and gene reporter systems. The M. smegmatis strain is ...
Hung, Hung-Chang et al. Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens. Braz J Infect Dis, June 2012, vol.16, no.3, p.242-249. ISSN 1413- ...
1-18 Ex. 1-17 & 18 Subgross and conventional histological images of ducts with extensive necrosis and a few remaining cancer cells. 1 continued The presence of fragmented casting type calcifications is a highly reliable radiological sign of malignancy. Preoperative microscopic diagnosis is necessary for optimum patient management. Histological−mammographic correlation helps us understand that the regions containing calcifications with high, even density and smooth contour will have the fewest viable malignant cells. The rectangles outline the cluster of calcifications. Ex. 4-3 Fragmented Casting Type Calcifications 33 Differential Diagnostic Problems Ex. 4-4 Specimen radiograph shows that the malignant type calcifications are distributed over a large area (rectangle). 40 mm 35 mm Ex. 4-4 Ex. 4-5 & 6 Histology: 40 mm × 35 mm Grade 3 solid and micropapillary DCIS with signs of epithelial−stromal interaction (periductal desmoplastic reaction and lymphocytic infiltration). Ex. 4-5 Ex. 4 ...
1A72: Active site modifications in a double mutant of liver alcohol dehydrogenase: structural studies of two enzyme-ligand complexes.
We would like to know if anyone knows what purpose the melted phenol crystals serve in the recipe from the AFIP manual for the Basic Fuchsin for Kinyouns method. Is it part of the stain or is it used as a preservative? Thanks for your help Richard Rodriguez Schering-Plough Research Institute Lafayette, NJ ...
These are high quality histopathology positive control tissues, positive for acid fast bacilli (AFB (Human Various)), with negative tissue. The first and last slide (2) are stained (Ziehl Neelsen) for authenticity before sending to you. These tissues are designed and specified for special stains, in hospitals, research, hand or machine staining.These tissues have not been tested…
Book Acid Fast Bacilli Susceptibility 5 Drugs Lj Blood @Home at Best Prices at the slot of your choice. View details of test: When to take, What is the normal range & Get reports Online.
Histological analysis revealed necrotising caseating granulomatous inflammation, and the Ziehl-Neelsen stain was positive for acid-fast bacilli. The subjects HIV status was not ascertained.. Case 2. A 21-year-old man had been nauseous and vomiting for one day, and had bought Gaviscon; he collapsed and died while vomiting into a toilet. He was thin but not emaciated. At autopsy, cardiomegaly was evident, with a firm, hollow, yellowish mass with a thrombus within the cavity, just below the aortic outflow tract in the left ventricle. The aortic valve cusps had been replaced by the mass. The lungs were normal and the lymph nodes enlarged. The subjects HIV status was not ascertained. No acid-fast bacilli were noted on Ziehl-Neelsen staining.. Discussion. Tuberculosis is rampant in South Africa and, with the exacerbating effects of AIDS, the incidence is likely to rise. Tuberculosis is generally thought to spare the heart, thyroid, pancreas and skeletal muscle; cardiovascular manifestations are ...
To compare the diagnostic sensitivity and specificity of seven Cryptosporidium diagnostic assays used in the UK, results from 259 stool samples from patients with acute gastrointestinal symptoms were compared against a nominated gold standard (real-time PCR and oocyst detection). Of the 152 true positives, 80 were Cryptosporidium hominis, 68 Cryptosporidium parvum, two Cryptosporidium felis, one Cryptosporidium ubiquitum and one Cryptosporidium meleagridis. The Cryptosporidium spp. diagnostic sensitivities of three Cryptosporidium and Giardia combination enzyme immunoassays (EIA) coupled with confirmation of positive reactions were 91.4-93.4 %, whilst the sensitivity of auramine phenol microscopy was 92.1 % and that of immunofluorescence microscopy (IFM) was 97.4 %, all with overlapping 95 % confidence intervals. However, IFM was significantly more sensitive (P = 0.01, paired test of proportions). The sensitivity of modified Ziehl-Neelsen microscopy was 75.4 %, significantly lower than those for the
1MGO: Mobility of Fluorobenzyl Alcohols Bound to Liver Alcohol Dehydrogenases as Determined by NMR and X-ray Crystallographic Studies
Tuberculosis (TB) is believed to affect nearly one third of the world population with approximately 9 million new cases detected every year. Current methods of diagnosis includes sputum smear microscopy, chest X-Ray and biopsy which are time consuming and expensive with a low level of accuracy.
Use as a counterstain during Ziehl-Neelsen and Kinyoun staining. Pro-Lab Diagnostics™ Malachite Green Stain is a ready-to-use stain to differentiate non-acid-fast organisms from mycobacterium. It is manufactured to the highest standards, according to all regulatory requirements, and CE labelled. 500 ML Acid fast Stain for Mycobacteria Malachite ...
I know that I have the correct bacteria because I did all the tests needed to determine which one I had. I had narrowed it down to 3 bacteria and planed to do an endospore stain next. My teacher told me that I could also do the acid fast stain, however he wouldnt let us actually carry it out. So he drew what I would see under the microscope. Which narrowed it down to 1, but to be on the safe side I did the endospore test anyway. He then told me that I made a big mistake and was going to be confused with what I would find ...
Benzophenoneidum 2465-27-2. Benzopinacol 464-72-2. Benzperidine hydrochloride 3626-66-2. ...
TY - JOUR. T1 - Evaluation of an antigen capture enzyme-linked immunosorbent assay for detection of Cryptosporidium oocysts. AU - Newman, R. D.. AU - Jaeger, K. L.. AU - Wuhib, T.. AU - Lima, A. A M. AU - Guerrant, R. L.. AU - Sears, Cynthia Louise. PY - 1993. Y1 - 1993. N2 - The diagnosis of the small (4- to 6-μm) Cryptosporidium oocysts is labor intensive and relies on stool concentration, with subsequent staining and microscopy. The primary purpose of this study was to evaluate the clinical utility of an antigen capture enzyme-linked immunosorbent assay (ELISA) (LMD Laboratories, Carlsbad, Calif.) in detecting Cryptosporidium oocysts in human stools. A total of 591 specimens (76 diarrheal, 515 control) obtained from 213 inhabitants of an urban slum in northeastern Brazil were examined by both ELISA and conventional microscopic examination (CME) of formalin-ethyl acetate-concentrated stool samples stained with modified acid-fast and auramine stains. Forty-eight diarrheal stools (63.2%) were ...
... benzophenoneidum MeSH D02.092.146.271 --- bromhexine MeSH D02.092.146.271.100 --- ambroxol MeSH D02.092.146.290 --- ...
Singh, S. K., Sengupta, S., Antony, R., Bhattacharya, S., Mukhopadhyay, C., Ramasubramanian, V., Sharma, A., Sahu, S., Nirkhiwale, S., Gupta, S., Rohit, A., Sharma, S., Raghavan, V., Barman, P., Sood, S., Mamtora, D., Rengaswamy, S., Arora, A., Goossens, H. & Versporten, A., 01-11-2019, In : Journal of Hospital Infection. 103, 3, p. 280-283 4 p.. Research output: Contribution to journal › Article ...
Fingerprint Dive into the research topics where Gordon Prescott is active. These topic labels come from the works of this person. Together they form a unique fingerprint. ...
Benzophenoneidum *Bromhexine + *Benzenaminium, 4,4-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide * ...
... , Biopsy, Fine-Needle , Female , Humans , Immunocompromised Host , Lung/pathology , Lung Diseases, Fungal/ ... Acridine Orange , Benzophenoneidum , Citrus sinensis , Darkness , Fluorescence , Leprosy, Multibacillary , Leprosy, ... Bacteriological Techniques , Benzophenoneidum/diagnosis , Coloring Agents/diagnosis , Humans , Microscopy/methods , Microscopy ... Animals , Male , Female , Cattle , Dogs , Benzophenoneidum , Staining and Labeling/methods , Cryptosporidiosis/diagnosis , ...
Benzophenoneidum , beta Carotene , bisphenol A , Bleomycin , Calcitriol , Capsaicin , Carboplatin , Chlorpyrifos , chromium ...
Acridine Orange , Benzophenoneidum , Citrus sinensis , Darkness , Fluorescence , Leprosy, Multibacillary , Leprosy, ... Acridine Orange , Bacteria , Benzophenoneidum , Diagnosis , Fluorescence , Humans , Laboratory Personnel , Leprosy , Microscopy ...
Benzophenoneidum. LinkOut - more resources. Full Text Sources. *Elsevier Science. Medical. *Mycobacterial Infections - ...

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