Chromosomes, Artificial, Yeast
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Genetic Complementation Test
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.
In Situ Hybridization, Fluorescence
Herpesvirus 3, Human
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Chromosomes, Human, Pair 3
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A live VACCINIA VIRUS vaccine of calf lymph or chick embryo origin, used for immunization against smallpox. It is now recommended only for laboratory workers exposed to smallpox virus. Certain countries continue to vaccinate those in the military service. Complications that result from smallpox vaccination include vaccinia, secondary bacterial infections, and encephalomyelitis. (Dorland, 28th ed)
A disorder characterized by recurrent episodes of paroxysmal brain dysfunction due to a sudden, disorderly, and excessive neuronal discharge. Epilepsy classification systems are generally based upon: (1) clinical features of the seizure episodes (e.g., motor seizure), (2) etiology (e.g., post-traumatic), (3) anatomic site of seizure origin (e.g., frontal lobe seizure), (4) tendency to spread to other structures in the brain, and (5) temporal patterns (e.g., nocturnal epilepsy). (From Adams et al., Principles of Neurology, 6th ed, p313)
Directories as Topic
Product Line Management
Biological Science Disciplines
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Sensitivity and Specificity
working with cosmids
... cells and we can make good cosmid DNA from them. However, after 3-4 weeks , the yield of cosmid DNA from cells derived from the ... It is NO NEED to pick up a single colony for your cosmid preparation each time. After you have checked and know that the cosmid ... Has anyone else had this problem? Are we leaving something out of the , media? Are the cosmid-containing cells less viable? , , ... working with cosmids. Barry Bowman bowman at biology.ucsc.edu Tue Jun 20 19:26:42 EST 1995 *Previous message: only test ...
Cosmid Medical Definition | Merriam-Webster Medical Dictionary
Medical definition of cosmid: a plasmid into which a short nucleotide sequence of a bacteriophage has been inserted to create a ... Resources for cosmid. Time Traveler: Explore other words from the year cosmid first appeared Time Traveler! Explore the year a ... Comments on cosmid. What made you want to look up cosmid? Please tell us where you read or heard it (including the quote, if ... Post the Definition of cosmid to Facebook Share the Definition of cosmid on Twitter ...
agrobacterium cosmid library
Interpreting cosmid overlaps with acedb
If I want to pick a good cosmid to inject for mutant rescue, how can I tell with acedb which cosmid will have the ORF lying ... I am interested in a predicted gene that seems to be at one end of a sequenced cosmid, perhaps even extending from one cosmid ... Interpreting cosmid overlaps with acedb. Curtis Loer cloer at acusd.edu Mon Aug 25 16:17:22 EST 1997 *Previous message: Cell ... could someone help me with interpreting the fingerprinting info to pick a few good cosmids? Thanks. Curtis M. Loer Dept. of ...
Cosmid - Wikipedia
Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or mammalian cosmids (linked to ... Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 ... Selection against wild type cosmid DNA is simply done via size exclusion. Cosmids, therefore, always form colonies and not ... is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases. Cosmids are ...
Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. - PubMed - NCBI
GenBank U53502, cosmid g17311
... "sequence overlap with cosmid g8261" misc_feature 7505..7506 /note="site of apparent internal deletion in cosmid" BASE COUNT ... REFERENCE 1 (bases 1 to 11862) AUTHORS Goodman,H.M. and Gallant,P. TITLE Sequence of cosmid g17311 from Arabidopsis thaliana ... LOCUS ATU53502 11862 bp DNA PLN 05-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g17311 DNA. ACCESSION U53502 ...
GenBank U53501, cosmid g8261
pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies
TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of ... High efficiency cosmid library construction is accomplished using easy and highly repr,biological,biology supply,biology ... TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies,The pWEB:: ... The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid ...
A cosmid-based system for constructing mutants of herpes simplex virus type 1. - PubMed - NCBI
One cosmid in set A contains the oriL locus in a nonoverlapping region and lacks ori+L forms. This set generated only ori-L ... Two cosmids common to sets B and C ostensibly contain one of the origins of viral DNA replication (oriL) in a region of overlap ... A cosmid-based system for constructing mutants of herpes simplex virus type 1.. Cunningham C1, Davison AJ. ... Cosmids containing large fragments of herpes simplex virus type 1 DNA were prepared using a vector that allows intact inserts ...
cosmid - Free People Search - Yasni.com
250 people for cosmid: High School, Busty, Babes, Galleries, TX, CA, Videos, Pics Galleries, Profile, University, free people ... 05.02.2012 Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid ... More of Holly Riley and other busty girls next door at Cosmid · Holly Riley Cosmid 1 Holly Riley Cosmid 2 Holly Riley Cosmid 3 ... Christine carollo cosmidhristine carollo cosmid: Christine carollo cosmidhristine carollo cosmid. Relaxinol ingredients. You ...
BDGP: Materials: Cosmid Clones
Cosmids were provided by the European Drosophila Genome Project (EDGP).. EDGP cosmids are available from Geneservice in the UK ... Cosmids are available either singly or on high-density filters.. The members of the European Drosophila Genome mapping ... Contact for cosmids:. Dr. I. Siden-Kiamos, Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete. Telephone ...
Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? - QIAGEN
When plasmids or cosmids are larger than 10 kb, pre-heat Buffer EB (or water) to 70°C prior to eluting DNA from the QIAprep ... All QIAprep Miniprep Kits can be used for preparation of low-copy number plasmids and cosmids up to 50 kb. Below are ... Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? ...
SDSATC Cosmid Prep
One colony: One library cosmid DNA miniprep. Because of the instability of the Olson cosmids, we have divised a miniprep ... Both the cosmid DNA and the E. coli DNAare denatured. However, only the cosmid DNA is supercoiled. Its two strands cannot ... The amount of cosmid DNA prepared is modest, but is more than enough to make an M13 library. While most genomists focus on the ... If you dont select the right E. coli colony, you cannot prepare the right cosmid DNA. 1-Late in your working day streak ...
Broad host range/E.coli cosmid vector pJQ210mp18 - incomplete.
Misc.Comments : Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - EcoRI/MCS/HindIII - P15A - cos - traJ - oriT - sacB.  Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C ...
pWEB- Cosmid Cloning Kit
The pWEB™ Cosmid Cloning Kit facilitates rapid and efficient construction of cosmid libraries using the pWEB Cosmid Vector (Fig ... pWEB™ Cosmid Cloning Kit. Notice: The pWEB Cosmid Cloning Kit has been discontinued.. As alternative, please consider the ... Figure 2 (click to enlarge). Outline of the cosmid cloning procedure using the pWEB-TNC™ and the pWEB™ Cosmid Cloning Kits.. ... The result is a complete and unbiased primary cosmid library.. Epicentre also offers kits for fosmid cloning and library ...
Vectors for plant transformation and cosmid libraries - CaltechAUTHORS
The cosmid vectors either contain a Hph marker (pCIT03), or a kanamycin-resistance marker (pCIT101-104). Three of the cosmid ... The others are all cosmid vectors which allow insertion of up to 46 kb of plant genomic DNA, and which also contain all of the ... The other two cosmid vectors (pCIT102 and pCIT104) carry restriction sites flanking the insertion site (XhoI) for convenient ... Hong Ma, Martin F. Yanofsky, Harry J. Klee, John L. Bowman, Elliot M. Meyerowitz, Vectors for plant transformation and cosmid ...
how to get cosmid DNA back to E.coli? - Molecular Cloning
how to get cosmid DNA back to E.coli? - (Aug/07/2006 ). Hi,. I need to know if it is possible to transform E.coli with cosmid ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I have used this method for transforming 21 kb cosmid. I hope this helps.. uhsna. quote name=puma date=Aug 7 2006, 12:27 PM ...
Epstein-Barr virus recombinants from overlapping cosmid fragments. | Journal of Virology
Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments. Message Subject (Your Name) has forwarded a page to you from ...
High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe
... a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, ... High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe Cell. 1993 Apr 9;73(1):109-20. doi: ... Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both ... and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping ...
Definition for cosmid
cosmid definition: Genetics A hybrid vector that has been spliced with plasmid DNA for cloning huge genes or gene fragments.; a ... Definition for "cosmid"*Genetics A hybrid vector that has been spliced… ... How would you define cosmid?. All the definitions on AZdictionary were written by people just like you. Nows your chance to ... Sentence for "cosmid"*E. coli and 25 µg/ml for… ... Medical Dictionary for "cosmid"*DNA from a bacterial ...
Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small...
The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and...
... nucleotide sequence of the 16,009-bp SacBII Kan domain of the P1 pAD10-SacBII cloning vector and the sequences of three cosmid ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ...
Isolation of coding sequences from bovine cosmids by means of exon trapping, Mammalian Genome | 10.1007/s003359900481 | DeepDyve
"Isolation of coding sequences from bovine cosmids by means of exon trapping, Mammalian Genome" on DeepDyve, the largest online ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Isolation of coding sequences from bovine cosmids ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Comincini, S.; Drisaldi, B.; Ferretti, L. ... Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for ...
Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. - Infoscience
... a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. The genome ... Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Eiglmeier, K ; Honoré, N ; Woods, ... In an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid ... Home , Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. ...
Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. | Infection and Immunity
The isolation and sample sequencing of a set of 11 cosmid clones that cover all of one and much of a second large PAI in the ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing.. D L Swenson, N O ... To identify possible genes unique to these PAIs, 100 independent subclones of the cosmids were made by PstI digestion and ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. ...
Get PDF - Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110
Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110 ... Cosmid-derived map of E.coli strain Bhb2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5070 ... Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5069 ... Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of Bacteriology 171(2): ...
Welcome to Cosmid - Sexy Amateurs, Busty Amateurs, Professional Photographers
Cosmids/YACs - WormBaseWiki
The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left ... C. elegans Cosmid and YAC Info. Ordering. Please submit all requests for C.elegans genomic clones via this request portal. [ ... Most Cosmids, Fosmids, YACs can be requested from Sanger via the form at the top of the page, ... Cosmid A*, B*, C*, D*, F*,J*,K*,M*,R*,T*,W*,Z* ... 1 C. elegans Cosmid and YAC Info. *1.1 Ordering. *1.2 ...
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