Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Any method used for determining the location of and relative distances between genes on a chromosome.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The functional hereditary units of BACTERIA.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.
A contiguous gene syndrome associated with hemizygous deletions of chromosome region 11p13. The condition is marked by the combination of WILMS TUMOR; ANIRIDIA; GENITOURINARY ABNORMALITIES; and INTELLECTUAL DISABILITY.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The type species of VARICELLOVIRUS causing CHICKENPOX (varicella) and HERPES ZOSTER (shingles) in humans.
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence A/AGCTT at the slash. HindIII is from Haemophilus influenzae R(d). Numerous isoschizomers have been identified. EC 3.1.21.-.
The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.
The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.
Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.
Elements of limited time intervals, contributing to particular results or situations.
Nonsusceptibility of a microbe to the action of ampicillin, a penicillin derivative that interferes with cell wall synthesis.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A live VACCINIA VIRUS vaccine of calf lymph or chick embryo origin, used for immunization against smallpox. It is now recommended only for laboratory workers exposed to smallpox virus. Certain countries continue to vaccinate those in the military service. Complications that result from smallpox vaccination include vaccinia, secondary bacterial infections, and encephalomyelitis. (Dorland, 28th ed)
An acute, highly contagious, often fatal infectious disease caused by an orthopoxvirus characterized by a biphasic febrile course and distinctive progressive skin eruptions. Vaccination has succeeded in eradicating smallpox worldwide. (Dorland, 28th ed)
Hostile conflict between organized groups of people.
A disorder characterized by recurrent episodes of paroxysmal brain dysfunction due to a sudden, disorderly, and excessive neuronal discharge. Epilepsy classification systems are generally based upon: (1) clinical features of the seizure episodes (e.g., motor seizure), (2) etiology (e.g., post-traumatic), (3) anatomic site of seizure origin (e.g., frontal lobe seizure), (4) tendency to spread to other structures in the brain, and (5) temporal patterns (e.g., nocturnal epilepsy). (From Adams et al., Principles of Neurology, 6th ed, p313)
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
Management control systems for structuring health care delivery strategies around case types, as in DRGs, or specific clinical services.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Research that involves the application of the natural sciences, especially biology and physiology, to medicine.
Ordered compilations of item descriptions and sufficient information to afford access to them.
... cells and we can make good cosmid DNA from them. However, after 3-4 weeks , the yield of cosmid DNA from cells derived from the ... It is NO NEED to pick up a single colony for your cosmid preparation each time. After you have checked and know that the cosmid ... Has anyone else had this problem? Are we leaving something out of the , media? Are the cosmid-containing cells less viable? , , ... working with cosmids. Barry Bowman bowman at biology.ucsc.edu Tue Jun 20 19:26:42 EST 1995 *Previous message: only test ...
Medical definition of cosmid: a plasmid into which a short nucleotide sequence of a bacteriophage has been inserted to create a ... Resources for cosmid. Time Traveler: Explore other words from the year cosmid first appeared Time Traveler! Explore the year a ... Comments on cosmid. What made you want to look up cosmid? Please tell us where you read or heard it (including the quote, if ... Post the Definition of cosmid to Facebook Share the Definition of cosmid on Twitter ...
... Denise Melanson dmmelans at unity.ncsu.edu Sat Dec 21 21:25:12 EST 1996 *Previous message: ... I am looking for a cosmid library of A. tumefaciens genome. I dont need the Ti plasmids, I actually want the genomic DNA from ...
If I want to pick a good cosmid to inject for mutant rescue, how can I tell with acedb which cosmid will have the ORF lying ... I am interested in a predicted gene that seems to be at one end of a sequenced cosmid, perhaps even extending from one cosmid ... Interpreting cosmid overlaps with acedb. Curtis Loer cloer at acusd.edu Mon Aug 25 16:17:22 EST 1997 *Previous message: Cell ... could someone help me with interpreting the fingerprinting info to pick a few good cosmids? Thanks. Curtis M. Loer Dept. of ...
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Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or mammalian cosmids (linked to ... Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 ... Selection against wild type cosmid DNA is simply done via size exclusion. Cosmids, therefore, always form colonies and not ... is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases. Cosmids are ...
Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA.. Rao RN, Richardson MA, Kuhstoss S. ...
... "sequence overlap with cosmid g8261" misc_feature 7505..7506 /note="site of apparent internal deletion in cosmid" BASE COUNT ... REFERENCE 1 (bases 1 to 11862) AUTHORS Goodman,H.M. and Gallant,P. TITLE Sequence of cosmid g17311 from Arabidopsis thaliana ... LOCUS ATU53502 11862 bp DNA PLN 05-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g17311 DNA. ACCESSION U53502 ...
LOCUS ATU53501 37570 bp DNA PLN 06-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g8261 DNA (cytosine-5-) ...
TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of ... High efficiency cosmid library construction is accomplished using easy and highly repr,biological,biology supply,biology ... TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies,The pWEB:: ... The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid ...
One cosmid in set A contains the oriL locus in a nonoverlapping region and lacks ori+L forms. This set generated only ori-L ... Two cosmids common to sets B and C ostensibly contain one of the origins of viral DNA replication (oriL) in a region of overlap ... A cosmid-based system for constructing mutants of herpes simplex virus type 1.. Cunningham C1, Davison AJ. ... Cosmids containing large fragments of herpes simplex virus type 1 DNA were prepared using a vector that allows intact inserts ...
250 people for cosmid: High School, Busty, Babes, Galleries, TX, CA, Videos, Pics Galleries, Profile, University, free people ... 05.02.2012 Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid ... More of Holly Riley and other busty girls next door at Cosmid · Holly Riley Cosmid 1 Holly Riley Cosmid 2 Holly Riley Cosmid 3 ... Christine carollo cosmidhristine carollo cosmid: Christine carollo cosmidhristine carollo cosmid. Relaxinol ingredients. You ...
Cosmids were provided by the European Drosophila Genome Project (EDGP).. EDGP cosmids are available from Geneservice in the UK ... Cosmids are available either singly or on high-density filters.. The members of the European Drosophila Genome mapping ... Contact for cosmids:. Dr. I. Siden-Kiamos, Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete. Telephone ...
When plasmids or cosmids are larger than 10 kb, pre-heat Buffer EB (or water) to 70°C prior to eluting DNA from the QIAprep ... All QIAprep Miniprep Kits can be used for preparation of low-copy number plasmids and cosmids up to 50 kb. Below are ... Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? ...
One colony: One library cosmid DNA miniprep. Because of the instability of the Olson cosmids, we have divised a miniprep ... Both the cosmid DNA and the E. coli DNAare denatured. However, only the cosmid DNA is supercoiled. Its two strands cannot ... The amount of cosmid DNA prepared is modest, but is more than enough to make an M13 library. While most genomists focus on the ... If you dont select the right E. coli colony, you cannot prepare the right cosmid DNA. 1-Late in your working day streak ...
Misc.Comments : Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - EcoRI/MCS/HindIII - P15A - cos - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C ...
The pWEB™ Cosmid Cloning Kit facilitates rapid and efficient construction of cosmid libraries using the pWEB Cosmid Vector (Fig ... pWEB™ Cosmid Cloning Kit. Notice: The pWEB Cosmid Cloning Kit has been discontinued.. As alternative, please consider the ... Figure 2 (click to enlarge). Outline of the cosmid cloning procedure using the pWEB-TNC™ and the pWEB™ Cosmid Cloning Kits.. ... The result is a complete and unbiased primary cosmid library.. Epicentre also offers kits for fosmid cloning and library ...
The cosmid vectors either contain a Hph marker (pCIT03), or a kanamycin-resistance marker (pCIT101-104). Three of the cosmid ... The others are all cosmid vectors which allow insertion of up to 46 kb of plant genomic DNA, and which also contain all of the ... The other two cosmid vectors (pCIT102 and pCIT104) carry restriction sites flanking the insertion site (XhoI) for convenient ... Hong Ma, Martin F. Yanofsky, Harry J. Klee, John L. Bowman, Elliot M. Meyerowitz, Vectors for plant transformation and cosmid ...
how to get cosmid DNA back to E.coli? - (Aug/07/2006 ). Hi,. I need to know if it is possible to transform E.coli with cosmid ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I have used this method for transforming 21 kb cosmid. I hope this helps.. uhsna. quote name=puma date=Aug 7 2006, 12:27 PM ...
Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments. Message Subject (Your Name) has forwarded a page to you from ...
... a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, ... High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe Cell. 1993 Apr 9;73(1):109-20. doi: ... Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both ... and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping ...
cosmid definition: Genetics A hybrid vector that has been spliced with plasmid DNA for cloning huge genes or gene fragments.; a ... Definition for "cosmid"*Genetics A hybrid vector that has been spliced… ... How would you define cosmid?. All the definitions on AZdictionary were written by people just like you. Nows your chance to ... Sentence for "cosmid"*E. coli and 25 µg/ml for… ... Medical Dictionary for "cosmid"*DNA from a bacterial ...
Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small ...
... nucleotide sequence of the 16,009-bp SacBII Kan domain of the P1 pAD10-SacBII cloning vector and the sequences of three cosmid ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ...
"Isolation of coding sequences from bovine cosmids by means of exon trapping, Mammalian Genome" on DeepDyve, the largest online ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Isolation of coding sequences from bovine cosmids ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Comincini, S.; Drisaldi, B.; Ferretti, L. ... Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for ...
... a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. The genome ... Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Eiglmeier, K ; Honoré, N ; Woods, ... In an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid ... Home , Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. ...
The isolation and sample sequencing of a set of 11 cosmid clones that cover all of one and much of a second large PAI in the ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing.. D L Swenson, N O ... To identify possible genes unique to these PAIs, 100 independent subclones of the cosmids were made by PstI digestion and ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. ...
Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110 ... Cosmid-derived map of E.coli strain Bhb2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5070 ... Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5069 ... Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of Bacteriology 171(2): ...
HOME TO THE FAMOUS COSMID GIRLS, REAL GIRLS, REAL PHOTOGRAPHERS, REAL LIFE - UPDATED AT LEAST ONCE EVERY STINKING DAY ...
The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left ... C. elegans Cosmid and YAC Info. Ordering. Please submit all requests for C.elegans genomic clones via this request portal. [ ... Most Cosmids, Fosmids, YACs can be requested from Sanger via the form at the top of the page, ... Cosmid A*, B*, C*, D*, F*,J*,K*,M*,R*,T*,W*,Z* ... 1 C. elegans Cosmid and YAC Info. *1.1 Ordering. *1.2 ...
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