Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Methods for maintaining or growing CELLS in vitro.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Techniques used in studying bacteria.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.
Substances capable of inhibiting, retarding or arresting the process of fermentation, acidification or other deterioration of foods.
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Proteins found in any species of bacterium.
A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.
Nurses professionally qualified in administration.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Selection of a type of occupation or profession.
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
Personal satisfaction relative to the work situation.
Chronic ESOPHAGITIS characterized by esophageal mucosal EOSINOPHILIA. It is diagnosed when an increase in EOSINOPHILS are present over the entire esophagus. The reflux symptoms fail to respond to PROTON PUMP INHIBITORS treatment, unlike in GASTROESOPHAGEAL REFLUX DISEASE. The symptoms are associated with IgE-mediated hypersensitivity to food or inhalant allergens.
The upward or downward mobility in an occupation or the change from one occupation to another.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Apparatus, devices, or supplies intended for one-time or temporary use.
Further or repeated use of equipment, instruments, devices, or materials. It includes additional use regardless of the original intent of the producer as to disposability or durability. It does not include the repeated use of fluids or solutions.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Treatment process involving the injection of fluid into an organ or tissue.
Endoscopes for examining the interior of the larynx.
Centers for acquiring, characterizing, and storing organs or tissue for future use.
The capacity to conceive or to induce conception. It may refer to either the male or female.
Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Clinical and laboratory techniques used to enhance fertility in humans and animals.
Diminished or absent ability of a female to achieve conception.
The process of laying or shedding fully developed eggs (OVA) from the female body. The term is usually used for certain INSECTS or FISHES with an organ called ovipositor where eggs are stored or deposited before expulsion from the body.
The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.
The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Diphosphoric acid esters of fructose. The fructose-1,6- diphosphate isomer is most prevalent. It is an important intermediate in the glycolysis process.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
The motion of air currents.
Rare leukoencephalopathy with infantile-onset accumulation of Rosenthal fibers in the subpial, periventricular, and subependymal zones of the brain. Rosenthal fibers are GLIAL FIBRILLARY ACIDIC PROTEIN aggregates found in ASTROCYTES. Juvenile- and adult-onset types show progressive atrophy of the lower brainstem instead. De novo mutations in the GFAP gene are associated with the disease with propensity for paternal inheritance.
A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective against most gram-positive bacteria and against gram-negative cocci. It has also been used as an experimental convulsant because of its actions on GAMMA-AMINOBUTYRIC ACID mediated synaptic transmission.

Evidence of exponential growth of an anammox population in an anaerobic batch culture. (1/89)

Twenty-five replicates of growth medium for anaerobic ammonium oxidation (anammox) containing (15)N-labeled ammonium and non-labeled nitrite were inoculated into an anammox enrichment culture at low density, and anaerobically incubated batchwise. In the headspace, (29)N(2) partial pressure linearly increased via anammox in 25 vials, confirming that anammox populations were viable in all subcultures. On prolonged incubation, exponential increases in (29)N(2) were not observed in all but 13 subcultures, suggesting that the anammox population may not proliferate unless all conditions for growth are satisfied. The estimated first-order rate coefficients in those 13 subcultures varied from 0.0029 to 0.0048 h(-1).  (+info)

A novel recirculating flow-perfusion bioreactor for periosteal chondrogenesis. (2/89)


Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains. (3/89)


Physico-chemical characteristics and functional properties of chitin and chitosan produced by Mucor circinelloides using yam bean as substrate. (4/89)


Evaluation of a laboratory-scale bioreactive in situ sediment cap for the treatment of organic contaminants. (5/89)


Optimisation of surface expression using the AIDA autotransporter. (6/89)


Parametric optimization of feruloyl esterase production from Aspergillus terreus strain GA2 isolated from tropical agro-ecosystems cultivating sweet sorghum. (7/89)

A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71- 0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of 30 degrees C. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions.  (+info)

Repeated-batch operation of immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor for lactose hydrolysis. (8/89)

In this study, we investigated the performance of an immobilized beta-galactosidase inclusion bodies-containing Escherichia coli cell reactor, where the cells were immobilized in alginate beads, which were then used in repeated-batch operations for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose over the long-term. In particular, in the Tris buffer system, disintegration of the alginate beads was not observed during the operation, which was observed for the phosphate buffer system. The o-nitrophenyl-beta-D-galactoside hydrolysis was operated successfully up to about 80 h, and the runs were successfully repeated at least eight times. In addition, hydrolysis of lactose was successfully carried out up to 240 h. Using Western blotting analyses, it was verified that the beta-galactosidase inclusion bodies were sustained in the alginate beads during the repeated-batch operations. Consequently, we experimentally verified that beta-galactosidase inclusion bodies-containing Escherichia coli cells could be used in a repeated-batch reactor as a biocatalyst for the hydrolysis of o-nitrophenyl-beta-D-galactoside or lactose. It is probable that this approach can be applied to enzymatic synthesis reactions for other biotechnology applications, particularly reactions that require long-term and stable operation.  (+info)

Biohydrogen technology has drawn much attention due to its many advantages. However, it is still necessary to screen much more strains with stronger hydrogen-producing capacity for future commercialization processes. In this paper, a biohydrogen-producing strain Enterobacter aerogenes EB-06 was isolated, identified, and named. It could convert glycerol to biohydrogen by microorganism fermentation. The effects of oxygen content, initial pH, initial glycerol concentration, and initial nitrogen source content on biohydrogen production process were investigated. The results have shown that biohydrogen generation was more favorable under anaerobic conditions. The optimum specific biohydrogen production rate (QH2) was obtained as 41.47 mmol H2/g DCW h at 40 g/L initial glycerol concentration. The optimum volume H2 yield (CH2) was 83.76 mmol H2/L at initial pH 7.0. It was found that nitrogen source content (0-4 g/L) could promote biohydrogen production and cell growth. The biohydrogen production of
High cell density fed batch and perfusion processes for stable non-viral expression of secreted alkaline phosphatase (SEAP) using insect cells: Comparison to a batch Sf-9-BEV system
A way of predicting the conditions of product formation in a two-state continuous cultivation on the basis of analyzing the batch process is described. The prediction applies to processes where the product formation is proportional to the growth rate and where the relationship between culture activity and the external conditions is linear. Using the example of glucose oxidase production byAspergillus niger the prediction is borne out. The difference between the predicted and found values does not exceed 15%.
Small-scale cultivation vessels, which allow fed-batch operation mode, become more and more important for fast and reliable early process development. Recently, the polymer-based feeding system was introduced to allow fed-batch conditions in microtiter plates. Maximum glucose release rates of 0.35 mg/h per well (48-well-plate) at 37 °C can be achieved with these plates, depending on the media properties. The fed-batch cultivation of fluorescent protein-expressing E. coli at oxygen transfer rate levels of 5 mmol/L/h proved to be superior compared to simple batch cultivations. However, literature suggests that higher glucose release rates than achieved with the currently available fed-batch microtiter plate are beneficial, especially for fast-growing microorganisms. During the fed-batch phase of the cultivation, a resulting oxygen transfer rate level of 28 mmol/L/h should be achieved. Customization of the polymer matrix enabled a considerable increase in the glucose release rate of more than 250% to up
The study is seeking to design a fully integrated biohydrogen production process using fermentation and bioelectrolysis. To date, it has shown for the first time that hydrogen yield and volatile fatty acids (VFA) in a continuous biohydrogen fermenter can be increased by using in situ electrochemical hydrogen separation, CO2 removal and VFA separation via electrodialysis. Results of the project have been presented at the APEC Biohydrogen conference in Malaka, Malaysia Dec 2014 and at the 72nd IEA-HIA ExCo meeting, Netherlands 2015.. Part 1 ...
In chemical kinetics and batch processes K variables are measured on the batches at regular time intervals. This gives a J×K matrix for each batch (J time points times K variables). Consequently, a set of N normal batches gives a three-way matrix of dimension (N×J×K). The case when batches have different length is also discussed. In a typical industrial application of batch modelling, the purpose is to diagnose an evolving batch as normal or not, and to obtain indications of variables that together behave abnormally in batch process upsets. Other applications giving the same form of data include pharmaco-kinetics, clinical and pharmacological trials where patients (or mice) are followed over time, material stability testing and other kinetic investigations. A new approach to the multivariate modelling of three-way kinetic and batch process data is presented. This approach is based on an initial PLS analysis of the ((N×J)×K) unfolded matrix ((batch×time)×variables) with local time used ...
In this paper, the improvement of a fed-batch fermentation from the point of view of an industrial xylanase production process is described. The Bacillus strain chosen for this study is able to produce high quantities of a xylanase that is suitable to be used as bleach boost agent in chlorine-free bleaching sequences of paper pulp. It was found that xylo-oligosaccharides hydrolysis products from xylan by xylanase action were indispensable for induction of the enzyme synthesis, but that their presence in quantities of only 0.1g dm^-3 xylose equivalents led to catabolite repression. A substrate-limited fed-batch process, that is the most adapted, was furthermore improved with regard to nutrient requirement of the microorganism, especially the nitrogen source. A process with constant supply of a culture medium containing xylan, peptone and mineral nitrogen was able to produce 20240nkatcm^-3 with a productivity of 910nkatcm^-3 h^-1, which places the process among the best ever reported. ...
The upstream stage of the production process involves searching for and extracting raw materials. The upstream part of the production process does not do anything with the material itself, such as processing the material. This part of the process simply finds and extracts the raw material. Thus, any industry that relies on the extraction of raw materials commonly has an upstream stage in its production process. In a more general sense, upstream can also refer to any part of the production process relating to the extraction stages.. ...
Bacillus sphaericus, an obligate alkalophile, overproduces extracellular alkaline proteases. Kinetics of growth and protease production by this organism were analyzed to identify parameters that control protease synthesis. Results showed protease production to be both growth and non-growth associated although a major portion of the enzyme was secreted in post exponential phases. The initial concentration of glucose and nitrogen sources significantly affected cell growth and enzyme secretion. Fed-batch cultures were performed to maximize protease activity in a bioreactor. By using suitable feeding strategies, the protease activity and its productivity in a fed-batch process was increased by 44% over that in batch process, primarily due to the longer maintenance of increased rates of growth and enzyme production by providing continuous and controlled supply of additional substrate and nutrients.. ...
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Due to recent legislation requiring the determining of the pharmacokinetic effect of both enantiomers separately, of any new racemic drug before commercialisation, much research is done to improve and optimise methods for obtaining chirally pure compounds important for the pharmaceutical industry, for example epoxide precursors. To date most experiments regarding the biocatalytic chiral separation of 1,2-epoxyoctane has been done in batch processes. The aim of this study was to optimise the enantioselective hydrolysis of 1,2-epoxyoctane by Rhodosporidiurn tondoides in both a batch and continuous process. The batch process was optimised in terms of stir speed, biomass (cell) concentration and reaction time, while the flow-through reactor (continuous process) was optimised with regards to the flow rate as a function of the pressure and the amount of chitosan and biomass in the reactor. Initial inconsistencies of epoxide concentrations in preliminary studies were found to be due to adsorption by ...
Graduates in molecular biology, bioengineering, and many other fields can potentially take advantage of many exciting and lucrative career opportunities in biotechnology development, manufacturing, commercial operations, business development, licensing, and investment fund management. To access such opportunities quickly and successfully, it is valuable to understand the basic principles of bioprocess engineering. Furthermore, a basic background in bioprocess engineering can be applied not only to a career in pharmaceutical biotechnology, but also to a career in many other areas of biotechnology, such as biofuels.. Bioprocess engineering is one the oldest and most successful professions. Success in large-scale recombinant protein manufacturing is a recent manifestation of a long and glorious history. Bioprocess engineering was and often still is a major skill set among those called brewer, winemaker, or in ancient times, wizard or sorcerer. The ability to successfully employ the magic of ...
Process intensification through continuous manufacturing has been practiced in the chemical, petrochemical, and food industries for years and has gained much interest among biopharmaceutical manufacturers (1). Key drivers encouraging biomanufacturers of therapeutic molecules to convert batch processes into continuous operation include flexibility, productivity, cost effectiveness, and product consistency. Continuous upstream processing has been demonstrated for the manufacture of a broad range of molecules, including complex/labile proteins such as enzymes (2) and monoclonal antibodies (3). Recent publications have reported successful application…. ...
Chemical plants typically rely on continuous and batch production processes, each posing different requirements for a control system. A continuous process calls for a robust and stable control system that will not fail and cause the shutdown of a production line, whereas the emphasis with a batch process is on having a control system that allows great flexibility in making adjustments to formulas, procedures, and the like. Both kinds of systems need to be managed in available quality history of product, and to be able to execute non-routine operations. With its extensive product portfolio, experienced systems engineers, and global sales and service network, Yokogawa has a solution for every plant process. | Yokogawa Slovakia
Hydrogen, an abundant element and this diatomic molecule is trending as an alternative energy source. Hydrogen can be produced as metabolic byproducts of fermen
Genedata Bioprocess™ is an industry-standard web application based on an Oracle® relational database infrastructure. It has been designed and optimized for processing, storing, and querying large and complex data volumes, specifically for the handling of large numbers of samples. As an open system, it enables seamless interfacing and integration with diverse laboratory instruments such as pipetting robots, colony pickers, and analytics devices. The Genedata Bioprocess software comes with significant built-in business logic and works out-of-the-box in its default configuration. The system can also be configured to support corporate-specific variants of the bioprocess R&D workflow - e.g., for the handling of custom sample nomenclature or integration with customer-specific lab automation set-ups. Public APIs enable straightforward integration with existing corporate IT infrastructure, such as data warehouses, request management systems, LIMSs, ELNs, or project management systems. API ...
Biopharmaceutical company Cleveland BioLabs Inc has appointed Elena Kasimova to its board of directors. Kasimova is a director of strategy and investment at BioProcess Capital Partners.. PRESS RELEASE. BUFFALO, NY-(Marketwired - Jan 28, 2015) - Cleveland BioLabs, Inc. (NASDAQ: CBLI) today announced the appointment of Elena Kasimova of BioProcess Capital Partners to the Companys Board of Directors. Ms. Kasimova was appointed pursuant to a Rights Agreement with Dr. Mikhail Mogutov, which was entered into in connection with a private sale of securities to Dr. Mogutov in June 2014. As a result of the June 2014 sale, Dr. Mogutov is a holder of approximately 9% of CBLIs outstanding shares of common stock.. Ms. Kasimova has more than 10 years of experience in various financial positions with a focus on corporate finance and mergers and acquisitions. She has been Director of Strategy and Investment at BioProcess Capital Partners since 2010. Ms. Kasimova also currently serves on the Board of Directors ...
The paper focuses on application of computer simulations in real-world manufacturing, and brings a case study of optimisation of manpower in a workplace manufacturing window components. The paper presents the workplace, and applies simulation to design an optimisation to the production process and manpower used ...
First, before machining the CNC parts, be sure to look at the production processing, clearly understand the location, appearance, and engineering drawing specifications of the metal parts to be processed, and understand the production processing of the next process. Before clamping the raw materials for production processing, the specifications of the materials should be accurately measured to see if they meet the requirements of the engineering drawings, and the placement should be carefully checked to see if it is consistent with the program orders. Self-checking should be performed immediately after the initial processing of the production process to make immediate adjustments to the deviating statistics. The key to the self-check is the part specification.. The whole process of machining mechanical parts for looseness; after preliminary processing and then before deep processing, check the appearance and specifications of the engineering drawing position: check the specification of the base ...
Researchers at the Fraunhofer Institute for Material and Beam Technology IWS in Dresden have developed a new battery cell production process that coats the electrodes of the energy storage cells with a dry film instead of liquid chemicals. This simplified process saves energy and eliminates toxic solvents. A paper on...
Digital technologies are transforming the production processes of industry in all sectors. The benefits associated with the digitization of production plants are already a reality and, beyond that, quantifiable.
There are the following steps of instant cereal grain beverages production process: quality control, roasting, extraction, drying and agglomeration, packaging and logistics.
These analysts get a private tour of the Tesla Gigafactory and theyre very impressed and highly confident with the production process.
A batch-to-batch optimal iterative learning control (ILC) strategy for the tracking control of product quality in batch processes is presented. The linear time-varying perturbation (LTVP) model is built for product quality around the nominal trajectories. To address problems of model-plant mismatches, model prediction errors in the previous batch run are added to the model predictions for the current batch run. Then tracking error transition models can be built, and the ILC law with direct error feedback is explicitly obtained. A rigorous theorem is proposed, to prove the convergence of tracking error under ILC. The proposed methodology is illustrated on a typical batch reactor and the results show that the performance of trajectory tracking is gradually improved by the ILC ...
A device and method develop a training record for use in creating an empirical model of a process from data associated with the process. Data from a continuous process in the form of a continuous file or data from a set of batch files associated with different runs of a batch process is used to develop a file having multiple values for each of a plurality of variables associated with the process. A flag or other indicator is placed within the file to indicate the location of a significant event within the process or the location at which the data from different batch files was concatenated together. A record is then developed from the file by selecting a multiplicity of values within the file. Thereafter, it is determined if any two of the selected values within the record are located within the file at positions which are on different sides of the position indicated by the flag or other indicator. If so, that record is prevented from being used as a training record for training an empirical model of
After color and tone, proper sharpening has the greatest impact on perceived image quality. As discussed in the camera section, most DSLR cameras require capture sharpening to overcome the blurring effects of the bayer array and the anti-aliasing filter it often requires to prevent moiré. Capture sharpening is easy to apply as a batch process in PIEware. It can also be applied in Photoshop as an individual or batch action using Unsharp Mask, Smart Sharpen filters, or sharpening plug-ins such as PhotoKit Sharpener, Nik Sharpener Pro, Focal Blade or other specialized tools.. A second round of sharpening, which we refer to as creative or process sharpening is usually done at the pixel editing stage during masterfile creation. A good example is when we might sharpen only some features in an image- such as eyes and hair, leaving skin unsharpened (or even blurred a bit).. A third round of sharpening which we refer to as output sharpening, is required by all image files regardless of reproduction ...
Process intensification through continuous manufacturing has been practiced in the chemical, petrochemical, and food industries for years and has gained much interest among biopharmaceutical manufacturers (1). Key drivers encouraging biomanufacturers of therapeutic molecules to convert batch processes into continuous operation include flexibility, productivity, cost effectiveness, and product consistency. Continuous upstream processing has been demonstrated for the manufacture of a broad range of molecules, including complex/labile proteins such as enzymes (2) and monoclonal antibodies (3). Recent publications have reported successful application…. ...
Cielo Waste Solutions (CSE:CMC) is delivering on its promise to build its first commercial-grade renewable diesel refinery. Construction is well underway at its High River facility - proving that its proprietary technology will be able to convert multiple different waste feed-stocks, including landfill waste, into high-grade, renewable diesel has moved the company from demonstration to commercial stage.. Vancouver-based Cielo has extended its private placement offering of units, to accommodate additional subscribers. The private placement has so far raised $2,283,236 for moving its development forward. A private lender has also signed a Term Sheet with Cielo extending the Company a $3.5 million line of credit. Currently, Cielo and the company are working thru the paperwork to get the credit facility finalized. The 356-litre-an hour continuous flow refinery that Cielo is currently building is a retrofit of a 50-litre-an-hour batch process demonstration plant, which the company and its license ...
Within the plant, all the process areas are also bunded, and the trade waste from there piped … XI-Detergents-A-Soap-13 Batch process soap. »More detailed ...
This is a prose version of a talk I gave at PyCon 2019; you can also watch a video of the talk. Youve run your batch process with your scientific model, and after hours and hours it spit out a result. And the result is wrong. You suspect theres a bug in the calculation, youre not sure what it is, and the slow feedback cycle is making debugging even harder. Wouldnt it be great if you could debug and speed up your program without having to spend days running it just to reproduce your problem? Now, Im not a scientist, Im a software engineer. But I did spend a year and a half working on scientific computing, and based on that experience Id like to offer a potential solution to this cluster of problems: logging, and in particular a logging library I and my coworkers found very helpful. But before I get to the solution, its worth considering where these problems come from: the specific characteristics of scientific computing.
Diffusion and mass transfer with chemical reaction; gas-liquid, gas-solid and liquid-liquid systems; analysis of mass transfer processes in metallurgical operations; mixing in continuous and batch processes. ...
Where is a ConTrack script? Go to the ConTrack section to see how to run it. ctrInit - Set up the diffusion data and the two regions of interest. This creates the candidate fiber tracks, but it does not score them. We typically track 100K and the time for this depends on the data (region of the brain). It could take a couple of hours. In a lousy region, it could take a day. Try a smaller number first to see whats happening. ctr_makeConTrackFiles.m - this is a script that batch processes the creation of these ROIs Scoring issues The track returns a set of candidate fibers. Many of these have kind of lousy scores. These are in a .pdb file. fg_projectname_logName.pdb This file can be opened in Quench. ctr_conTrackBaatchScore.m The scoring is contrack_score.glxa65 -i ctrSampler.txt Quench wont let you have some fibers with and some fibers without. ...
EntityStream is a real-time data flow that allows for performing complex data improvements within it. The main purpose of it is to present the user with up-to-date information and keep the state of data updated. Using a stream as a mechanism to solve a use case is generally more effective and faster at solving the problem than building complex batch processes relying on massive infrastructure.
I want to take a text pages and apply an overlay over the data an save the resulting form as a PDF document. I have over 100,000 pages like this so I want some kind of batch process to take … Continue reading →. ...
H01L24/96-Batch processes at chip-level, i.e. with connecting carried out on a plurality of singulated devices, i.e. on diced chips the devices being encapsulated in a common layer, e.g. neo-wafer or pseudo-wafer, said common layer being separable into individual assemblies after connecting ...
Corning has been at the forefront of upstream single-use technology and single-use adherent and suspension cell culture products provide reliable, predictable scale-up in bioprocess and cell therapy.
This book is divided into five parts that feature novel and key concepts and applications in Bioprocess Engineering field. The book endeavors to help academia, researchers, and practitioners to use the principles and tools of Chemical and Bioprocess Engineering.
July 26-30, 2021 For the second time, the 2021 Cellular Bioprocess Technology Short Course will be offered online. The online version of the course will be professionally broadcasted live to attendees. The efficient information and knowledge delivery that this course is known for will continue in an online format. The course covers fundamentals and practical analysis of cell culture technology and bioprocess engineering.
Are you going to Bioprocess International in Boston 2014? The Bioprocess International Conference offers complete programming coverage of the full bioprocessing continuum - featuring 65 innovative case studies and 70 new data presentations intended for companies of all sizes and at every stage of development. If you are planning to attend, please check out these activities and events you wont want to miss.
Perfusion processes enable continuous operation over extended periods of time by constantly providing fresh nutrients for the cells and simultaneously removing spent media and waste products, as compared to batch and fed-batch processes. This article explores the key benefits of perfusion processes. ...
There are two production processes for LSAW pipe: JCOE process and RBE process.. The advantages of JCOE production process are that it can produce more than 30mm thick steel pipe, which is suitable for the production of extra thick wall steel pipe; the disadvantages are low production efficiency (10 pieces / hour) and large investment. It is usually used for the production of straight seam submerged arc steel pipe with diameter range of Φ 508 - Φ 1422mm, wall thickness of 8-40mm and length of 10-12.5m.. RBE production process has the advantages of high production efficiency (15 pieces / hour) and small investment; the disadvantage is that the maximum wall thickness is not more than 30mm. It is usually used for the production of straight seam submerged arc steel pipe with diameter range of Φ 508 - Φ 1422mm, wall thickness of 6-30mm and length of 10-12.5m.. ...
Production processes in Industry 4.0 settings are usually highly automated. However, many complicated tasks, such as machine maintenance, must be executed by human workers. In current smart factories, such tasks can be supported by Augmented Reality (AR) devices. These AR tasks rely on high numbers of contextual factors like live data from machines or work safety conditions and are mostly not well integrated into the global production process. This can lead to various problems like suboptimal task assignment, over-exposure of workers to hazards like noise or heat, or delays in the production process. Current Business Process Management (BPM) Systems (BPMS) are not capable of readily taking such factors into account. There- fore, this contribution proposes a novel approach for context- integrated modeling and execution of processes with AR tasks. Our practical evaluations show that our AR Process Framework can be easily integrated with prevalent BPMS. Furthermore, we have created a comprehensive ...
Pan, X., Streefland, M., Dalm, C., Wijffels, R.H. & Martens, D.E. (2016). Selection of chemically defined media for CHO cell fed-batch culture processes. Cytotechnology, 69(1), 39-56. doi: 10.1007/s10616-016-0036-5 ...
|i|Bioprocess Engineering (BE)|/i| provides an international peer-reviewed forum to facilitate the discussion in the wide area of cell bioprocessing, biomedical devices, bioproducts, biotransformation, biological waste treatment, materials and energy, stem cell cultivation, biocontrol science, molecular enzymology, etc. The submission of manuscripts that meet the general criteria of significance and scientific excellence are encouraged.
Sept. 2, 2010, Toronto - SunOpta has sold SunOpta BioProcess Inc. (SBI) to Mascoma Corporation, bringing together SBIs fibre preparation and pretreatment technology with Mascomas consolidated bioprocessing technology.
Press release - TMR Research - Bioprocess Technology Market Comprehensive Business Study and Key Player Insights By 2025 -Philips Healthcare, Becton, Thermo Fisher Scientific Inc. - published on
Watch a webinar describing a new system that enables fully automated extraction and quantification of cellular DNA from bioprocess samples
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Get Bioprocess Monitoring PowerPoint Template with creative backgrounds and 20 expert-quality slides from and create jaw-dropping PPT presentation. Template 10760
REGULATORY INFORMATION TSCA Inventory Status All ingredients are listed on the TSCA inventory. Revision date: 04/01/2016 TA1 (METHYL ORANGE) No. (methyl orange), 6.1, pg III 15. Methyl Orange, Sodium Salt, ACS (547 … Moreover, the degradation efficiency of as-synthesized NPs was assessed using Methyl Orange (MO). Toxicity to Animals: Acute oral toxicity (LD50): 60 mg/kg [Rat]. Methyl Orange MSDS; Methyl Orange Indicator AR; Methyl Orange … Toxicity study revealed the degradation of MG into non-toxic products by K. rosea. California Proposition 65 Not Listed SARA 302 Not Listed SARA 304 Not Listed SARA 311 Methyl Orange Toxicity No additional information available 12.2. The removal of such type of dye-stuffs from effluent is necessary as such effluents cause abnormal coloration to the surface water and block photosynthetic bacteria and aquatic plants from sunlight. IATA/ICAO UN3143, Dyes, solid, toxic, n.o.s. Acute toxicity - fish This mixture is not classified as toxic to aquatic organisms. ...
Sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. So it is can be used as a sugar source in many processes. Lignin and hemicellulose must be removed before hydrolysis of cellulose. Several different pretreatment approaches have been studied. The purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatment methods for improving enzymatic digestibility of sugar cane bagasse in pilot plant scale. Sugar cane Bagasse was washed and pretreated by sulfuric acid (BA) 0.1 %, sodium hydroxide (BSH) 0.1% and (BS) steam was done at pressure 2 bar. Sugar cane bagasse was also treated by sulfuric acid (1, 2, 2.5, and 5%) at 121 º C and 151 º C. Ozone and ozone-acid methods also was used for delignification of bagasse. Particle diameter of bagasse in all pretreatment methods was 3-4 mm. All pretreated bagasse was hydrolyzed by cellulose enzyme complex and beta-gluocosidase at pH 5 and temperature 45ºC. Glucose and xylose
Box-Behnken design to optimize the synthesis of new crosslinked chitosan-glyoxal/TiO2 nanocomposite: Methyl orange adsorption and mechanism studies … This alkaline solution also eliminates the possible phenol formed by conversion in the water soluble phenoxide anion. Search chemicals by name, molecular formula, structure, and other identifiers. Apparatus: Round bottomed flask - 500 ml, Reflux condenser. Synthesis of Methyl Orange - Reaction Mechanism - Free download as PDF File (.pdf), Text File (.txt) or read online for free. FURTHER COMMENTS. Methyl orange is an azo dye produced from a synthesis reaction between diazonium ions and aromatic structures. Therefore, it requires us to find an effective method to deal with the … Sodium Salt And Nitrosonium Ion. Lets now transition into talking about how methyl red is synthesized in the lab by organic chemists. Synthesis of Methyl Orange - Reaction Mechanism Aim: To prepare methyl orange from sulphanilic acid. Direct filtration of the latter is ...
Wholesale Mataas Na Density Cell Culture Technique from China, Need to find cheap Mataas Na Density Cell Culture Technique as low price but leading manufacturers. Just find high-quality brands on Mataas Na Density Cell Culture Technique produce factory, You can also feedback about what you want, start saving and explore our Mataas Na Density Cell Culture Technique, Well reply you in fastest ...
You searched for: Exhibit Tags bacgen1 Remove constraint Exhibit Tags: bacgen1 Genre Letters (correspondence) Remove constraint Genre: Letters (correspondence) Subject Escherichia coli Remove constraint Subject: Escherichia coli Subject Cell Culture Techniques Remove constraint Subject: Cell Culture Techniques ...
1,3-Propanediol (1,3-PDO) is important building blocks for the bio-based chemical industry, Klebsiella pneumoniae can be an attractive candidate for their production. However, 1,3-PDO production is high but productivity is generally low by K. pneumoniae. In this study, repeated fed-batch cultivation by a lactate and 2,3-butanediol (2,3-BDO) deficient mutant of K. pneumoniae were investigated for efficient 1,3-PDO production from industrial by-products such as crude glycerol. First, optimal conditions for repeated fed-batch fermentation of a ΔldhA mutant defective for lactate formation due to deletion of the lactate dehydrogenase gene (ldhA) were determined. Maximal 1,3-PDO production level and productivity obtained by repeated fed-batch fermentation under optimized conditions were 81.1 g/L and 3.38 g/L/h, respectively, and these values were successfully maintained for five cycles of fermentation without any loss of fermentation capacity. This results were much higher than that of the normal fed-batch
During pregnancy, oxygen diffuses from maternal to fetal blood through villous trees in the placenta. In this paper, we simulate blood flow and oxygen transfer in feto-placental capillaries by converting three-dimensional representations of villous and capillary surfaces, reconstructed from confocal laser scanning microscopy, to finite-element meshes, and calculating values of vascular flow resistance and total oxygen transfer. The relationship between the total oxygen transfer rate and the pressure drop through the capillary is shown to be captured across a wide range of pressure drops by physical scaling laws and an upper bound on the oxygen transfer rate. A regression equation is introduced that can be used to estimate the oxygen transfer in a capillary using the vascular resistance. Two techniques for quantifying the effects of statistical variability, experimental uncertainty and pathological placental structure on the calculated properties are then introduced. First, scaling arguments are used to
PROCESS. DYNAMICS & CONTROL By: RALP JAYSON L. ALDAY ChE - THE DEVELOPMENT OF A MATHEMATICAL MODEL 2. OUTLINE Additional Examples of Mathematical Modeling Modeling Difficulties. ADDITIONAL EXAMPLES OF MATHEMATICAL MODELING Example 1. Mathematical model of a CSTR Consider a continuous stirred tank reactor system. A simple reaction A B takes place in the .
Quasi Vivo Training - Theory and practice of advanced cell culture techniques through a combination of lectures, demonstrations and practical experience.
New York-based SiGNa Chemistry is developing rechargeable cartridges that could provide an alternative wireless charging strategy for mobile electronics.The cartridge, called mobile-H2, contains a substance called sodium silicide that produces hydrogen when it comes in contact with water.
Read β-MnO2 microrods for the degradation of methyl orange under acid condition from aqueous solutions, Research on Chemical Intermediates on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
DASGIP Parallel Bioreactor Systems for R&D and process development in both cell culture and microbiology allow for advanced bioprocess control and automation. Parallel processing, precise control of all relevant parameters, user-defined profiles and innumerable automation features result in accelerated and highly efficient process development. Our DASware software solutions support DoE, process historians and comprehensive data management. Configurable solutions address the unique requirements of microbial, phototrophic, mammalian and human cells, stem cell applications, as well as biofuel and biopolymer processes.. ...
Fed-batch processes are very common and widely used in industry due to their success in obtaining high cell density cultures during the fermentation of E. coli. Processes such as the one designed are critical in the development of biotechnology-derived products, which have had an increasing market demand over the last two decades. As the demand for such products continues to rise, a need for scale-up of therapeutic proteins such as eGFP will likely ensue, and a process such as the one proposed in this work will prove to be an excellent training tool for those aiming to gain experience in this very relevant biomanufacturing process ...
Bakers yeast, Saccharomyces cerevisiae, as one of the most often used workhorses in biotechnology has been developed into a huge family of application optimised strains in the last decades. Increasing numbers of strains render their characterisation highly challenging, even with the simple methods of growth-based analytics. Here we present a new sensor system for the automated, non-invasive and parallelisable monitoring of biomass in continuously shaken shake flask cultures, called CGQ (
to the reactor and it is run until a predetermined set point (i.e. time, concentration). The bioreactor has a constant volume (the initial volume).  Fed-Batch: The bioreactor is a batch process in the beginning and after a certain point a feed input is introduced and the volume of the vessel increases.  Continuous: The bioreactor starts with an initial volume and media is constantly introduced and product is constantly taken out. The inputs and outputs are at the same rate, so the volume always remains the same ...
However, in the case of HRTFB, the relation was linear for surface renewal over a wide range of values. Shake flasks and un-baffled stirred vessels with surface aeration were not suitable for such a study, since the surface area in either system was difficult to define and varied as the shaker or stirrer/agitator speed was changed. In addition, the surface-to-volume ratio was unfavorable for obtaining high oxygen transfer rates.. Wetted-wall columns have been used for gas-liquid transfer studies. However, the rate of flow down or up the wetted wall is limited by gravitational force, and the requirement for recirculation of the fermentation liquid dictated an aseptic pumping operation.. Heaviside Theory (HST) based Concept: In oxic bioprocess engineering system in liquid culture transfer of oxygen from air to liquid has been measured by fast response steam sterilizable dissolved oxygen probe on line. In the measurement, the concept of flow of oxygen from higher concentration (heaviside) to lower ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Bioprocess Engineering, Third Edition is a comprehensive update of the worlds leading introductory textbook on biochemical and bioprocess engineering. Drs. Michael L. Shuler, Fikret Kargi, and Matthew DeLisa review the relevant fundamentals of biochemistry, microbiology, and molecular biology, introducing key principles that enable bioprocess engineers to achieve consistent control over biological activity.
The capacitance probe only measures the viable cell mass and is therefore ideal for this application and it has been applied for process control in sono-perfused cytostats, spin-filter perfused bioreactors and for maintaining steady-state, continuous culture of bioreactors with external loop filters (eg the Repligen ATF system) for monoclonal antibody and recombinant protein production.. In the second example of process control, data are taken from the FDA (Division of Biotechnology Review and Research-II OBP/OPQ/CDER). 5-L glass bioreactors were run in batch, fed batch and perfusion modes. In the perfusion mode the bioreactor was equipped with an XCell ATF device (Repligen) and a capacitance probe. A photograph of the experimental set up is shown in Figure #3.. In Figure #4, the viable cell densities for cultures run in the three different modes are compared between the Nova Bioprofile method (based on image analysis and Trypan blue) and the Aber values. The Aber FUTURA VCD is based on ...
Bioprocess Science is a subject that you will study as a part of your bachelor programme at Roskilde University. The bachelor subject Bioprocess Science is an expanding interdisciplinary field that draws upon multiple areas of knowledge, but especially the molecular, environmental and medical biologic subjects together with chemistry.. You will acquire a solid understanding of key theories and principles of bioprocess science including industrial processes, biological products, drug development together with screening and selecting organisms that are optimal for biological production of specified products for medical, industrial, aquaculture or agriculture application. The subject engages in research into, among other things, usage of different model organisms (zooplanktons, insects, microorganisms including genetically modified organisms etc.) in the production of biological products such as alternative protein, omega fatty acids, pharmaceuticals and enzymes. The subject will introduce ...
Article 4. Production processes and methods of analysis for aromatised wine products. 1. Aromatised wine products shall be produced in accordance with the requirements, restrictions and descriptions laid down in Annexes I and II.. 2. The Commission shall be empowered to adopt delegated acts in accordance with Article 33 concerning the establishment of authorised production processes for obtaining aromatised wine products, taking into account consumers expectations.. In establishing the authorised production processes referred to in the first subparagraph, the Commission shall take into account the production processes recommended and published by the OIV.. 3. The Commission shall, where necessary, adopt, by means of implementing acts, methods of analysis for determining the composition of aromatised wine products. Those methods shall be based on any relevant methods recommended and published by the OIV, unless they would be ineffective or inappropriate in view of the objective pursued. Those ...
trailer 0 Several companies own licenses to an ammonia production process; however, they share many common steps . 0000007865 00000 n > The reaction is reversible and the production of ammonia is exothermic. >> /Font 46 0 R /Resources
The FASTpress suite is the perfect solution for use in production process analysis and supports function and quality control in production
The FASTpress suite is the perfect solution for use in production process analysis and supports function and quality control in production
The amblr® 250 modular bioreactor system is an expandable benchtop workstation, which offers a simple approach to process development for fermentation and cell culture.. Sartorius Stedim Biotech (SSB) introduced the ambr® 250 modular to offer bioprocess scientists access to advanced benchtop bioreactor technology for process development. This system combines a unique single-use bioreactor vessel and consists of a workstation with 2, 4, 6, or 8 single-use bioreactors, with a working volume range of 100mL to 250mL.. These mini bioreactors are based on the same stirred tank bioreactors in the well-established ambr® 250 high-throughput system and contain impellers suitable for fermentation or cell culture. They are also fully integrated with liquid reservoirs and syringe pumps, allowing rapid experimental set up and turn around, significantly increasing lab efficiency.. The system brings simplicity to the lab bench. By following three easy steps, a bioreactor and all required accessories can be ...
An extrusion system that can create a polymer/gas solution rapidly for continuous processing of microcellular plastics is presented. Microcellular plastics are characterized by cell densities greater than 109 cells/cm3 and fully grown cells smaller than 10 μm. Previously these microcellular structures have been produced in a batch process by saturating a polymeric material with an inert gas under high pressure followed by inducing a rapid drop in the gas solubility. The diffusion phenomena encountered in this batch processing is typically slow, resulting in long cycle times. In order to produce microcellular plastics at industrial production rates, a means for the rapid solution formation is developed. The processing time required for completing the solution formation in the system was estimated from experimental data and the dispersive mixing theory based on an order-of-magnitude analysis. A means for promoting high bubble nucleation rates in the gas-saturated polymer via rapid heating is also ...
Nitrite accumulation is not unusual in batch processes such as sequencing batch reactor (SBR) with high-strength of ammonium or nitrate wastewaters. A possible mechanism of nitrite inhibition on Acinetobacter was depicted in a biochemical model, which the protonated species, nitrous acid form of nit...
Catalog Record: GCKP84 -- general chemical kinetics code for gas-phase flow and batch processes including heat transfer effects , HathiTrust Digital ...
I recently participated in my first community chat in the Etsy Virtual Labs. It started out as an excuse to kill some time on a quiet Friday in the office plus I figured I could probably pick up a few tips. The topic was Time Management and Organization for the Holidays and it was run by etsys Beth Ferreira. It was set up as a webcast with Beth on a webcam and the participants typing in their questions/comments. She had also prepared handy slides for us to follow. In her presentation she addressed the specific challenges of getting organized (time-consuming tasks, email/phone distractions, etc) and how to tackle these things head on. She also emphasized the importance of setting goals for your business, making lists (and actually sticking to them) and batch processes. Probably the most valuable piece of advice to took from it was the concept of creating a calendar- blocking off specific time to create, time to ship, time to do bookkeeping, etc. instead of sporadically doing things ...
This macro was designed to measure the size of the scratch wound in a wound scratch assay. It uses an edge-detection and thresholding technique.. It will batch process all images in a directory. Images captured by time-lapse should be compiled into stacks using a tool similar to Metamorph nd & ROI files importer (nd stack builder) by Fabrice P. Cordelières. Images to be analyzed should be placed in one directory (Source Directory). A second directory should be created to save results files and images (Destination Directory). Setting correct Lower and Upper thresholds is important to obtain a good result. Two macros are available, one using edge detection, the second one using background subtraction. ...
In statistics, restricted randomization occurs in the design of experiments and in particular in the context of randomized experiments and randomized controlled trials. Restricted randomization allows intuitively poor allocations of treatments to experimental units to be avoided, while retaining the theoretical benefits of randomization. For example, in a clinical trial of a new proposed treatment of obesity compared to a control, an experimenter would want to avoid outcomes of the randomization in which the new treatment was allocated only to the heaviest patients. The concept was introduced by Frank Yates (1948)[full citation needed] and William J. Youden (1972)[full citation needed] as a way of avoiding bad spatial patterns of treatments in designed experiments. Consider a batch process that uses 7 monitor wafers in each run. The plan further calls for measuring a response variable on each wafer at each of 9 sites. The organization of the sampling plan has a hierarchical or nested ...
Researchers are increasingly turning to 3D cell culture techniques for their studies, due to the improved physiological relevance of the cellular environment. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which allows researchers to culture cell types of their choice in an in vivo like environment. In this poster it is shown that the Tecan EVO freedom can be used to reliably automate 3D cell culture preparation. RAFT 3D cell cultures main
Thawing - should occur quickly and cells diluted in pre-warmed culture medium to prevent any toxic effects of cryoprotectants in super-zero temperatures. Incubate and examine (phase contrast) the next day. Cells may need to be washed in media if cryoprotectant has a known adverse cytopathic effect ...
Korean Cell Line Bank. 18th KCLB Workshop. Basic Cell Culture Techniques Hands-on Workshop. 국가목적형 소재은행인 한국세포주은행에서 아래와 같이 18차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop을 개최합니다. 참가자는 10명내외의 인원으로 구성하여 하루동안 이론교육 및 실습이 진행됩니다. 접수는 11월 23일까지이며, 참가여부는 개별통지하며, 무료교육입니다.. 관심있으신 연구자께서는 한국세포주은행 홈페이지(서비스-,Hands-on Workshop)에서 신청하여 주시고, 문의사항은 전화(02.3668-7915), 이메일([email protected])로 문의하여 주시기 바랍니다.. 감사합니다.. ...
DASGIP Parallel Bioreactor Systems for R&D and process development in both cell culture and microbiology allow for advanced bioprocess control and automation. Parallel processing, precise control of all relevant parameters, user-defined profiles and innumerable automation features result in accelerated and highly efficient process development. Our DASware software solutions support DoE, process historians and comprehensive data management. Configurable solutions address the unique requirements of microbial, phototrophic, mammalian and human cells, stem cell applications, as well as biofuel and biopolymer processes.. ...
Limestone Crushing Processing Flow Sheet Stone Crushers China. Flow diagram of crushing plants. Sep 08, 2018 production process flow chart sinolinking gold ore production process flow chart mining equipment us 5133 27311 set mobile crushing plant production process flow chart get price pe400600 jaw crusher machine price small stone crusher 1.
The goal of cell culture process intensification is to increase volumetric productivity, generally by increasing viable cell density (VCD), cell specific productivity or production bioreactor utilization in manufacturing. In our previous study, process intensification in fed-batch production with higher titer or shorter duration was demonstrated by increasing the inoculation seeding density (SD) from ~ 0.6 (Process A) to 3-6 × 106 cells/mL (Process B) in combination with media enrichment. In this study, we further increased SD to 10-20 × 106 cells/mL (Process C) using perfusion N-1 seed cultures, which increased titers already at industrially relevant levels by 100% in 10-14 day bioreactor durations for four different mAb-expressing CHO cell lines. Redesigned basal and feed media were critical for maintaining higher VCD and cell specific productivity during the entire production duration, while medium enrichment, feeding strategies and temperature shift optimization to accommodate high VCDs were also
Advanced cell culture techniques and scale-up offer exciting research opportunities; however, they also come with complex challenges and questions. Corning Life Sciences knows cells - you might even say its in our DNA with over 100 years of experience in...
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
6 Bioreactor System Jobs available on topEngineer, the worlds largest engineering job site. Search and apply online today. Page 1 of 6
We create custom bioreactor systems for a variety of applications and culture types such as blood vessel culture, hollow organ and more.
Find moving bed bioreactor system articles on Environmental XPRT, the worlds largest environmental industry marketplace and information resource.
The Eppendorf DASbox Mini Bioreactor System is a premium tool that helps streamlining process development. Its innovative design in blocks of four makes it an ideal tool for parallel processing and De
In 1956 Kerst sent that, if two Oscillations of aggregates could Join improved in looking cells, it should be American to verify substrates in which one download Putting Biotechnology to Work: Bioprocess digitized with another fulfilling in the pdfAutoerotic impairment. world of this content is the practice of seen teams in proteins killed ligase companies( relate below Colliding-beam age states). The highest awesomnala Beginnings Perhaps low-income protect improved based by the ubiquitin of this program.
BioProcess International Europe is the leading conference in Europe for connecting science, technology and business to optimise bioprocessing. By attending, you will access the innovation you need to accelerate your mAbs, vaccines and novel products towards commercial success. Europes Leading BioProcessing Event for Accelerating Biologics, mAbs, Vaccines and Novel Products to Commercial Success Access Leading Science, Technologies and Contacts Across the Entire Bioprocessing Spectrum, All from the Comfort of Your Home! The virtual platform will remain open for 30 days post-event for registered attendees.
BioProcess International Europe is the leading conference in Europe for connecting science, technology and business to optimise bioprocessing. By attending, you will access the innovation you need to accelerate your mAbs, vaccines and novel products towards commercial success. Europes Leading BioProcessing Event for Accelerating Biologics, mAbs, Vaccines and Novel Products to Commercial Success Access Leading Science, Technologies and Contacts Across the Entire Bioprocessing Spectrum, All from the Comfort of Your Home! The virtual platform will remain open for 30 days post-event for registered attendees.
Genedata Bioprocess is a first-in-class, off-the-shelf enterprise software solution that makes large-molecule development and CMC activities more efficient and improves process quality
The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly subject to stress ... the potency of each batch of penicillin varied from batch to batch. It was therefore impossible to prescribe 1 g of penicillin ... This causes the cell wall to weaken due to fewer cross links and means water uncontrollably flows into the cell because it ... This is because Gram-positive bacteria do not have an outer cell membrane and are simply enclosed in a thick cell wall. ...
The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly subject to stress ... This weakens the cell wall of the bacterium, and osmotic pressure becomes increasingly uncompensated-eventually causing cell ... Gram-positive bacteria are called protoplasts when they lose their cell walls. Gram-negative bacteria do not lose their cell ... Bacteria constantly remodel their peptidoglycan cell walls, simultaneously building and breaking down portions of the cell wall ...
Their batch of cultures lasted from 1912 to 1946. Because the tissue cultures far outlived their organisms, they were deemed " ... At Yale, Burrows also successfully established tissue cultures of embryonic chick cells. Upon returning to Carrel's laboratory ... One significant innovation was the method they developed for serial activation of tissues in culture. This technique ... "Culturing Life: How Cells Became Technologies". Harvard University Press, Cambridge. Burrows, Montrose T., Johnston, Charles G ...
2. High cell density (High cell concentration)[1] In a batch culture, to achieve very high cell concentrations, e.g. 50-100 g ... Fed-batch culture is a powerful technique to do so. Examples of the repressible promoter are trp promoter and phoA promoter. 7 ... fed-batch culture is superior to conventional batch culture when controlling concentrations of a nutrient (or nutrients) ... This type of the fed-batch culture is named constantly-fed-batch culture (CFBC), and is well established mathematically and ...
They were the first calves to be produced using standard cell-culturing techniques. In 2001, Brazil cloned their first heifer, ... the first batch of monotocous cloned police dogs was born. In 1958, John Gurdon, then at Oxford University, explained that he ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell. 172 (4): 881-887.e7. doi:10.1016/j.cell. ... In 1986, the first mouse was cloned in the Soviet Union from an embryo cell. The first mouse from adult cells, Cumulina, was ...
Generally performs with a constant error rate as an off-line (batch) type process. A range of image classification techniques ... To quantify the number of cells in a culture, the cells can be simply plated on a petri dish with growth medium. If the cells ... require cell counting. Calculating the fraction of dead to live cells as a measure of cell viability, such as of cells exposed ... Cell suspensions are turbid. Cells absorb and scatter the light. The higher the cell concentration, the higher the turbidity. ...
Cell culture[edit]. Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering ... "To address the H9N2 threat, NIAID contracted with Chiron Corporation to produce investigational batches of an inactivated ... Cell culture (cell-based) manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines ... In contrast, cell culture manufacturing technology can be applied to influenza vaccines as they are with most viral vaccines (e ...
The technique will leverage computer assisted design models, co-culture media which support muscle and fat cells as well as ... Fed-batch systems mean inoculating the cells, culturing them and harvesting them in one distinct period. Stirred tank ... Cultured meat is meat produced by in vitro cell culture of animal cells, instead of from slaughtered animals. It is a form of ... the density of the cells on the scaffold means that not all cells have an interface with culture media, leading to cell death ...
There would need to be a way to determine how many cells survived at the end of the incubation period using batch cultures. ... The improved pipetting technique was described in 2011 in the study of the biosafety level 3 (BSL-3) pathogen Bacillus ... such as a delay due to the time required for the repair of damaged cell structures such as cell walls or cell membranes, are ... At this time, cells exposed to no antimicrobial agent were added to several wells of the plate from the culture kept on ice; ...
Finally, cultured cells are poured into the dish for plasmid uptake. However, with the invention of different types of ... Reverse transfection is a technique for the transfer of genetic material into cells. As DNA is printed on a glass slide for the ... Effectene Reagent spontaneously forms micelle structures exhibiting no size or batch variation (as may be found with pre- ... The addition and attachment of target cells to the DNA-loaded surface can lead to a higher probability of cell-DNA contact, ...
Non-viral spontaneous hole formation in cell culture (e.g. LLC-PK1, or the human gingival epithelial cell culture model, Gie- ... Special computer systems have been designed with the ability to scan samples in batches. The appearance of the plaque depends ... or using techniques such as staining (e.g. neutral red for eukaryotes or giemsa for bacteria) or immunofluorescence. ... Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models. Trends in Cell ...
Culture-media conditioned by macrophages can be used. Production in cell culture is usually preferred as the ascites technique ... The antibody-containing medium is then incubated with the immobilized antigen, either in batch or as the antibody is passed ... single B cell culture, single cell amplification from various B cell populations and single plasma cell interrogation ... similar to a cancer cell). This mixture of cells is then diluted and clones are grown from single parent cells on microtitre ...
In 2006, Schärfe System was acquired by Innovatis AG, a company focused on cell culture analysis. CASY utilizes the techniques ... It would not be necessary to detect the cells from a batch to another batch but measure them continuously and smoothly. CASY ... As the cell size is related to the cell volume, a cell size profile in cell population can be produced in terms of pulse height ... This technology can be applied for cell counting, cell culture analysis at a certain time interval, or even a period of time. ...
... could be used to identify cell cultures infected with virus and also cultures with antibodies to virus. This pH could be easily ... 1. He proved a method for separation of monkey kidney cells, which led to techniques for large scale production. 2. He ... The measurement developed by Youngner for safely and quickly testing batches of vaccine and also antibodies to the virus after ... Youngner demonstrated the separation of monkey kidney cells using the pancreatic enzyme trypsin, a technique previously proven ...
Researchers have also aimed to improve cultivation techniques using a high-biomass batch culturing technique. One of the most ... This bacteria is a gram negative kidney-bean-shaped cell that deposits iron oxides on the concave side of the cell, forming ... These cultivation techniques follow those found in Emerson and Floyd (2005). Recently, researchers have been able to culture ... Barco, R. A.; Edwards, K. J. (2014). "Interactions of proteins with biogenic iron oxyhydroxides and a new culturing technique ...
... the fermenting mass of fruit-called the motor or kicker in prison parlance-is retained from batch to batch to make the ... Food hoarding in the inmate cells in both prisons and jails allows the inmates to acquire ingredients and produce pruno. During ... Though popularized in prison fiction, these techniques are slow and laborious, and generally result in a low alcohol content. ... Bum wine Changaa Chicha Drinking culture Jenkem Kilju Kvas Moonshine (hooch) Poitín Bootlegging Tepache Tharra White Lightning ...
... to restart due to a chemical compound released by dying yeast cells that inhibit the future growth of yeast cells in the batch ... There are various techniques that a winemaker can employ to minimize the chances of a stuck fermentation happening. The most ... such as adding nitrogen to the must in the form of diammonium phosphate or using cultured yeast with a high temperature and ... to a chemical compound released by the dying yeast that serves as an inhibitor to the growth of future yeast cells in the batch ...
As they are manufactured using recombinant bacterial production processes, the batch-to-batch consistency is maintained for ... Evans D, Johnson S, Laurenson S, Davies AG, Ko Ferrigno P, Wälti C (2008). "Electrical protein detection in cell lysates using ... The ability to direct in vitro screening techniques allows the identification of highly specific, high affinity Affimer binders ... proteins have been generated and shown to yield titrimetric volumes in the range of 200-400 mg/L under small scale culture ...
Ongoing research is performed to develop in situ separation techniques, where product is removed from the batch during the ... Whole cells are typically used when a reaction requires a co-factor. Although co-factors may be generated in vitro, it is ... Extracellular enzymes are released into the culture medium, and are much simpler to separate. Enzymes must maintain their ... These immobilization techniques occur at ambient temperatures in mild conditions, which have limited potential to modify the ...
Basic techniques. and tools. Biology field. *Bioreactor. *Cell culture. *Flow cytometry. *Hybridoma technology ... In mammals, major cell types include skin cells, muscle cells, neurons, blood cells, fibroblasts, stem cells, and others. Cell ... Cell wall. Further information: Cell wall. Many types of prokaryotic and eukaryotic cells have a cell wall. The cell wall acts ... Main article: Cell division. Cell division involves a single cell (called a mother cell) dividing into two daughter cells. This ...
... higher in dead cells than living cells. In terms of environmental remediation, biosorption is preferable to bioaccumulation ... It is made by carbon sequestration, which uses the opposite technique as for creating activated carbon. It is made by heating ... Fouladi Fard, Reza.; Azimi, A.A.; Nabi Bidhendi, G.R. (April 2011). "Batch kinetics and isotherms for biosorption of cadmium ... cleanup has been around since the early 1900s when Arden and Lockett discovered certain types of living bacteria cultures were ...
Modern knowledge of molluscan reproductive cycles has led to the development of hatcheries and new culture techniques. A better ... The chemoreceptor cells taste the water and are sensitive to touch. They are typically found near the siphons, but in some ... A single female oyster can produce 50-80 million eggs in a batch so the selection of broodstock is of great importance. The ... Culture of clams, cockles and ark shells more than doubled over this time period from 2,354,730 to 4,885,179 tons. Culture of ...
"Kantai Collection". Culture Japan. Danny Choo. 2013-10-10. Retrieved 2015-02-12. Kantai Collection: Ship Girl Catalogue (艦隊これくし ... Ryōta Momoi's 4koma manga will be released in print book format under the title KanColle 4 cell comic: Fubuki, I will do my ... of the initial retail batch stock was sold out within the first week. Kadokawa Games has announced that the game will be no ... where senior girls teach battle techniques to their juniors. It has been updated weekly since October 2013. ...
HCMV replicates within infected endothelial cells at a slow rate, taking about 5 days in cell culture. Like other herpesviruses ... An ELISA technique for CMV-specific IgM is available, but may give false-positive results unless steps are taken to remove ... The major disadvantage of the pp65 assay is that only a limited number of samples can be processed per test batch. CMV should ... Phenotypic resistance testing involves cultivation of the virus in cell culture and testing its susceptibility using different ...
Immunohistochemistry and immunocytochemistry are methods used to determine in which cells or parts of cells a particular ... Related techniques are also useful in sub-typing lymphocytes which all look quite similar on light microscopy. In laboratory ... Now, many are monoclonal, i.e. produced in tissue culture. Monoclonal antibodies that consist of only one type of antibody tend ... to provide greater antigen specificity, and also tend to be more consistent between batches. The first step in immunoperoxidase ...
This is done to avoid the sudden cold shock that the yeast cells may experience if the starter culture was added directly to ... wine regions is the ripasso method of adding the leftover pomace from the pressing of other wines into a newly fermenting batch ... their winery may not have this same assurance of survival factors and may need to compensate with other winemaking techniques.[ ... Re-hydrating freeze dried yeast cultures[edit]. Preparing a yeast starter culture and gradually cooling the culture down to the ...
Such systems can be microbial fuel cells or microbial electrolysis cells. In the absence of dissolved oxygen, nitrite, or ... Strous, M.; Heijnen, J.J.; Kuenen, J.G.; Jetten, M.S.M. (1998). "The sequencing batch reactor as a powerful tool for the study ... All have the taxonomical status of Candidatus, as none were obtained as classical pure cultures. Known species are divided over ... The cost reduction compared to conventional nitrogen removal is considerable; the technique is still young but proven in ...
Stem cells have the advantage over glial cells because they are able to proliferate more easily in culture. However, it remains ... Currently, cells can be differentiated across cell lines with yields ranging from 10% to 90% depending on techniques used. More ... The number of channels created was consistent from batch to batch and the variations in fiber diameter could be reduced by ... Schwann cells, astrocytes, and olfactory ensheathing cells. In addition to glial cells, stem cells also have potential benefit ...
High-throughput cell culture experiments, for example, testing the behavior of bacteria, finding rare cell types, directed ... and cell screening are suitable for using the droplet-based microfluidic techniques. Materials, incubation and viability ... Benefits of synthesis using this regime (compared to batch processes) include high throughput, continuous experiments, low ... Some of the challenges of cell culture in droplet-based microfluidics are common to other microfluidic culture system. First, ...
Electrolysis cells can be either open cell or closed cell. In open cell systems, the electrolysis products, which are gaseous, ... Fleischmann and Pons and co-workers met with Jones and co-workers on occasion in Utah to share research and techniques. During ... In Undead Science, sociologist Bart Simon gives some examples of cold fusion in popular culture, saying that some scientists ... and some batches of palladium never reach it because the pressure causes cracks in the palladium, allowing the deuterium to ...
Stem cell transplants are a recent research target, because stem cells are easy to manipulate and stem cells transplanted into ... The appearance of parkinsonism in a group of drug addicts in the early 1980s who consumed a contaminated batch of the synthetic ... Other effective techniques to promote relaxation include slow rotational movements of the extremities and trunk, rhythmic ... Society and culture. Cost. "Parkinson's awareness" logo with red tulip symbol. The costs of PD to society are high, but precise ...
Basic techniques. and tools. Biology field. *Bioreactor. *Cell culture. *Flow cytometry. *Hybridoma technology ... Modern usage also includes genetic engineering as well as cell and tissue culture technologies. The American Chemical Society ... It includes the practice of using cells such as microorganisms, or components of cells like enzymes, to generate industrially ... A rose plant that began as cells grown in a tissue culture ... For example, this technique was used with corn to produce the ...
The ease of culturing houseflies, and the relative ease of handling them when compared to the fruit fly Drosophila, have made ... She lays batches of about 100 eggs on decaying organic matter such as food waste, carrion, or feces. These soon hatch into ... During the Second World War, the Japanese worked on entomological warfare techniques under Shirō Ishii. Japanese Yagi bombs ... 96 Because the somatic tissue of the housefly consists of long-lived postmitotic cells, it can be used as an informative model ...
Before the development of synthetic pigments, and the refinement of techniques for extracting mineral pigments, batches of ... Gage, John (1999). Color and Culture: Practice and Meaning from Antiquity to Abstraction. University of California Press. ISBN ... In biology, a pigment is any colored material of plant or animal cells. Many biological structures, such as skin, eyes, fur, ... Doerner, Max (1984). The Materials of the Artist and Their Use in Painting: With Notes on the Techniques of the Old Masters, ...
It is believed that LSD is not usually produced in large quantities, but rather in a series of small batches. This technique ... doi:10.1016/j.cell.2016.12.033. PMC 5289311. PMID 28129538.. *^ a b c d e Shulgin A, Shulgin A (1997). "LSD". TiHKAL. Berkeley ... "Open Culture. September 1975. Retrieved April 27, 2019.. *^ Penner J. "Blowing The Philosopher's Fuses: Michel Foucault's LSD ... "Cell. 168 (3): 339-341. doi:10.1016/j.cell.2017.01.012. PMC 5520807. PMID 28129534.. ...
In cultureEdit. Main article: Insects in culture. Scarab beetles held religious and cultural symbolism in Old Egypt, Greece and ... At least a few insects have nociceptors, cells that detect and transmit signals responsible for the sensation of pain.[49][not ... Humans regard certain insects as pests, and attempt to control them using insecticides, and a host of other techniques. Some ... They can produce glue and protective substances for coating eggs or tough coverings for a batch of eggs called oothecae. ...
A batch of PPD created in 1939 serves as the US and international standard, called PPD-S.[4] PPD-S concentration is not ... concentrated cultures. It was first described by Robert Koch in 1890 and then Giovanni Petragnani.[citation needed] ... standardized for multiple-puncture techniques, and should be designed for the specific multiple-puncture system. [5] ... Giant multinucleated cell. *Pott disease. *Canga's bead symptom. *Prosector's wart. *Latent tuberculosis ...
"Journal of Biomolecular Techniques. 18 (5): 277. PMC 2392988 . PMID 18166670.. *^ Ankeny RA (June 2003). "Sequencing the genome ... Yeast (Saccharomyces cerevisiae) has long been an important model organism for the eukaryotic cell, while the fruit fly ... While traditional microbiology and microbial genome sequencing rely upon cultivated clonal cultures, early environmental gene ... these machines can sequence up to 96 DNA samples in a single batch (run) in up to 48 runs a day.[52] ...
Society and culture[edit]. Economics[edit]. This article needs to be updated. Please update this article to reflect recent ... meaning that the acetylcholine vesicles can't bind to the intracellular cell membrane,[50] preventing the cell from releasing ... After working out techniques for freeze-drying, buffering with albumin, and assuring sterility, potency, and safety, Scott ... stability and potency testing of Botox in response to increasing public concern that LD50 testing was required for each batch ...
Lander, Eric S. (2016-01-14). "The Heroes of CRISPR". Cell. 164 (1-2): 18-28. doi:10.1016/j.cell.2015.12.041. ISSN 1097-4172. ... The Dutch sauerkraut industry found that inoculating a new batch of sauerkraut with an old batch resulted in an excessively ... Katz, Sandor Ellix (2003). Wild Fermentation: The Flavor, Nutrition, and Craft of Live-Culture Foods. Chelsea Green Publishing ... Aubert, Claude (1999). Keeping Food Fresh: Old World Techniques & Recipes. Chelsea Green Publishing Company. ISBN 1-890132-10-1 ...
... or to preserve cultures genetically or phenotypically, as cell divisions can lead to mutations or sub-culturing can cause ... Controlled-rate and slow freezing, also known as slow programmable freezing (SPF),[14] is a set of well established techniques ... after the successful pregnancy of an embryo from the same batch three years earlier.[30] Many studies have evaluated the ... From a fresh culture plate, one single colony of interest is chosen and liquid culture is made. From the liquid culture, the ...
"Cell. 146 (6): 855-8. doi:10.1016/j.cell.2011.08.024. PMC 3414217 . PMID 21907397.. ... Typically, one batch of medicinals is prepared as a decoction of about 9 to 18 substances.[112] Some of these are considered as ... Techniques employed may include thumb presses, rubbing, percussion, and assisted stretching.. Qigong[edit]. Main article: ... Concepts of the body and of disease used in TCM have notions of a pre-scientific culture, similar to European humoral theory.[7 ...
Ancient cultures saw the Earth as the centre of the Solar System or universe (geocentrism). In the 16th century, Nicolaus ... History of chemo-informatics - history of the use of computer and informational techniques, applied to a range of problems in ... History of Flow chemistry - history of the chemical reaction is run in a continuously flowing stream rather than in batch ... and cells by means of the methods of mechanics. ... and required invention to devise the techniques, abstractions, ...
A handheld microfluidic cell culture incubator capable of heating and pumping cell culture solutions has also been developed. ... Manipulating cell seeding density is a common biological technique in controlling cell-cell interactions, but controlling local ... which reduces human error and operation costs Microfluidic technology is relatively economical due to batch fabrication and ... Cell fate is regulated by both interactions between stem cells and interactions between stem cells and membrane proteins. ...
... insect cells or mammalians cells can also be used.[114] These techniques are used to produce medicines such as insulin, human ... Narayanaswamy, S. (1994). Plant Cell and Tissue Culture. Tata McGraw-Hill Education. pp. vi. ISBN 978-0-07-460277-5. .. ... Embryonic stem cells incorporate the altered gene, which replaces the already present functional copy. These stem cells are ... As only a single cell is transformed with genetic material, the organism must be regenerated from that single cell. In plants ...
This technique is called escanciar un culín (also echar un culín) and is done to get air bubbles into the drink (espalmar), ... This leaves dead yeast cells and other undesirable material at the bottom of the old vat. At this point, it becomes important ... From the 1980s, government and gastronomic associations have worked to revive this culture in all Basque regions. Known as ... for fermentation introduces variability to the cider making process that makes it more difficult to generate multiple batches ...
Society and culture[edit]. Economics[edit]. This article needs to be updated. Please update this article to reflect recent ... It is translocated into the host cell cytoplasm where it cleaves the host protein SNAP-25, a member of the SNARE protein family ... After working out techniques for freeze-drying, buffering with albumin, and assuring sterility, potency, and safety, Scott ... stability and potency testing of Botox in response to increasing public concern that LD50 testing was required for each batch ...
Schramm, Ken (2003). The Compleat Meadmaker: Home Production of Honey Wine From Your First Batch to Award-winning Fruit and ... Because of the techniques used in its creation, short mead shares some qualities found in cider (or even light ale): primarily ... Racking is done for two reasons: it lets the mead sit away from the remains of the yeast cells (lees) that have died during the ... The terms "mead" and "honey-wine" often are used synonymously.[13][14] Some cultures, though, differentiate honey-wine from ...
Air cell. The larger end of the egg contains an air cell that forms when the contents of the egg cool down and contract after ... A batch of tea eggs with shell intact soaking in a brew of spices and tea. ... Bird eggs have been valuable foodstuffs since prehistory, in both hunting societies and more recent cultures where birds were ... "Digestibility of Cooked and Raw Egg Protein in Humans as Assessed by Stable Isotope Techniques". The Journal of Nutrition. 128 ...
... have proven to be effective knockdown tools in developmental biology experiments and RNA-blocking reagents for cells in culture ... Gene expression knockdown techniquesEdit. The expression of genes can be reduced by a variety of means, for example by using ... A Xenopus laevis female with a batch of freshly laid eggs and a Xenopus tropicalis male ... "Cell. 131 (5): 980-993. doi:10.1016/j.cell.2007.09.027. ISSN 0092-8674. PMC 2200633. PMID 18045539.. ...
"Cell. 134 (3): 416-426. doi:10.1016/j.cell.2008.06.021. ISSN 0092-8674. PMC 2602844. PMID 18692465.. ... "The new batch - 150,000 years ago". The evolution of man. London: BBC Science & Nature. Archived from the original on 2006-01- ... "The Hand How Its Use Shapes the Brain, Language, and Human Culture". The New York Times. Pantheon Books. Retrieved 2 July 2017. ... After 350,000 BP the more refined so-called Levallois technique was developed, a series of consecutive strikes, by which ...
Although in cultured cells ORF3 has not appeared essential for HEV RNA replication, viral assembly, or infection, it is ... The first vaccine batches came out of Innovax' factory in late October 2012, to be sold to Chinese distributors.[76] ... NAT is a technique used to screen blood molecularly, when blood donations are received; it screens for TTI.[74] ... Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ...
This makes it useful for some of the techniques of printing solar cells, electronics etc. ... Graphic screenprinting is widely used today to create mass or large batch produced graphics, such as posters or display stands ... and the non-professional look of such DIY culture screenprints have become a significant cultural aesthetic seen on movie ... Stencilling techniques[edit]. A macro photo of a screen print with a photographically produced stencil. The ink will be printed ...
The winemakers decide on which fining agents are used and these may vary from product to product and even batch to batch ( ... Proteins from the grape are broken down and the remaining yeast cells and other fine particles from the grapes are allowed to ... cultured yeast is often added to the must. One of the main problems with the use of wild ferments is the failure for the ... a technique known in Germany as süssreserve. ... Different batches of wine can be mixed before bottling in order ...
In popular culture[edit]. A pig has been used as a plot device in three James Bond films: Diamonds Are Forever, where Bond ... Usually pigging is done at the beginning and at the end of each batch, but sometimes it is done in the midst of a batch, such ... One of the most common and versatile is the foam pig which is cut or poured out of an open cell polyurethane foam into the ... Guided wave testing, another nondestructive testing technique that can be used for pipeline inspection ...
The cellular bulge produced by a haploid yeast cell towards a cell of the opposite mating type during the mating of yeast is ... S.W.I.N.E.: Capp used Li'l Abner to satirize current events, fads, and ephemeral popular culture (such as zoot suits in "Zoot ... When a batch "needs more body," the formidable pair simply goes out and clubs one (often a moose), and tosses it in. Over the ... contained serious analyses of Capp's narrative technique, his use of dialogue, self-caricature and grotesquerie, the strip's ...
In the TLFB process, cell death rate was low and constant and reached 4% dead cells at the end of cultivation (about 150 hours ... technique is described and used for Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed ... batch (MLFB) technique. A recombinant fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix ... The lower cell death rate in the TLFB correlated with a lower protease activity in the culture supernatant. The specific ...
This study reports on the effects of fermentor agitation and fed-batch mode of operation on citric acid production from Candida ... Cell Culture Techniques. Citric Acid / isolation & purification, metabolism*. Fermentation. Paraffin / metabolism*, ... Application of multiple fed-batch feedings can be used to extend the batch fermentation and increase final citric acid ... improvement over normal batch operation. The three-cycle fed-batch mode of operation also increased the final citric acid ...
At the same time, a technological radiation of microfluidic cell culture device designs is currently in progress. Ultimately, ... the utility of microfluidic cell culture will be determined by i … ... Culture of cells using various microfluidic devices is becoming more common within experimental cell biology. ... Batch Cell Culture Techniques / instrumentation* * Biocompatible Materials / chemistry* * Cells, Cultured * ...
Batch Cell Culture Techniques. *Biomass. *Citric Acid Cycle. *Ergosterol/metabolism. *Glycolysis. *Hydrogen-Ion Concentration ... Mitochondrial impairment could also reflect cell hypoxia at the 10,000 L scale culture resulting in changes in ergosterol ... Mitochondrial impairment could also reflect cell hypoxia at the 10,000 L scale culture resulting in changes in ergosterol ... Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch ...
Understanding of media and buffer preparation requirements for cell culture, and aseptic processing or lab techniques ... Ability to read, execute and document steps in a GMP batch record ... Understanding of media and buffer preparation requirements for cell culture, and aseptic processing or lab techniques ... Manufacture cell culture media preparation in support of S12 ide cel CAR T manufacturing per Standard Operating Procedures ( ...
We replicated the starvation state through long-term batch culture of G. sulfurreducens, where both electron donor and TEA were ... In survival phase, G. sulfurreducens persisted at a constant cell count for as long as 23 months without replenishment of ... We replicated the starvation state through long-term batch culture of G. sulfurreducens, where both electron donor and TEA were ... We replicated the starvation state through long-term batch culture of G. sulfurreducens, where both electron donor and TEA were ...
Batch Cell Culture Techniques All Science Journal Classification (ASJC) codes. *Agricultural and Biological Sciences(all) ... lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The ... lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The ... lactis in suspended-cell batch culture. All models were designed so that they contained biologically relevant parameters. The ...
It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. ... cells derived from higher eukaryotes such as mammals, birds, and insects). ... This section provides useful hints for culturing animal cells (i.e., ... How to make and transform competent cells, how to culture and handle plasmid-containing cells, and commonly used techniques for ...
Finally, it was demonstrated that viral infection of HeLa cells grown in 4-L bioreactor batch cultures resulted in increased ... Finally, it was demonstrated that viral infection of HeLa cells grown in 4-L bioreactor batch cultures resulted in increased ... Finally, it was demonstrated that viral infection of HeLa cells grown in 4-L bioreactor batch cultures resulted in increased ... Finally, it was demonstrated that viral infection of HeLa cells grown in 4-L bioreactor batch cultures resulted in increased ...
... and developing alternative techniques (e.g., cell culture grown virus, genetically engineered vaccines). Placing applications ... Modifying vaccine-control procedures to decrease delays in releasing batches of vaccines in diverse countries with similar ... Laboratory diagnosis included the isolation in cell culture of a virus that was identified locally as influenza type A but ... By August, further investigation with serologic and molecular techniques in the Netherlands (9, 20, 21) and in the United ...
... request pricing from manufacturers of Cell Culture Research Services , Cell / Tissue Culture ... Custom Batch Neurons. BrainXell. Custom production of neurons to fit your research goals. Read more... ... This service provides easy access to proven and comprehensive validation techniques. Promegas proprietary PowerPlex® and Ge ... Cell Line Authentication Service. Promega Corp.. Promega has partnered with ATCC to offer unparalleled STR testing service for ...
EFFECT OF CELL CULTURE PROCESS CHANGE FROM FED-BATCH TO CONTINUOUS ON PRODUCTIVITY AND PRODUCT QUALITY Integrated continuous ... Traditional techniques such as light obscuration often lack the sensitivity to distinguish translucent and potentially harmful ... Effect of Cell Culture Process Change from Fed-batch to Continuous on.... EFFECT OF CELL CULTURE PROCESS CHANGE FROM FED-BATCH ... Additionally, the cell culture medium (days in culture) for all small scale, pilot and clinical batches are analyzed by AAA to ...
Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. ... Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Together ...
An understanding and demonstrated competence in aseptic techniques/mammalian cell culture.. *A working knowledge of the proper/ ... Perform peer reviews of data, reports, and batch records.. *Use and maintain scientific equipment such as centrifuges, laminar ... Conduct basic technical assignments in the performance of in vitro or cell culture studies. ... Ability to learn and demonstrate competence in a variety of laboratory techniques. ...
The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly subject to stress ... the potency of each batch of penicillin varied from batch to batch. It was therefore impossible to prescribe 1 g of penicillin ... This causes the cell wall to weaken due to fewer cross links and means water uncontrollably flows into the cell because it ... This is because Gram-positive bacteria do not have an outer cell membrane and are simply enclosed in a thick cell wall. ...
Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell ... A simple linear factor was found to be responsible for the transferability of biomass models between cell lines, indicating a ... Errors of transferred models in the declining phase of the culture range for linear models around +100% or worse, causing ... control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. ...
This course is designed for those that have some knowledge of cell culture techniques but would like to specifically learn how ... Trainees will prepare and run a steam in place cycle on a stainless steel bioreactor and then inoculate a production batch into ... This course would be ideally suited to graduates with a cell culture background or cell culture technicians from a biologics ... During this course trainees will be introduced to cell culturing techniques using small scale shake flasks. Trainees will then ...
Fed-Batch Bioreactor Process Scale-Up From 3-L to 2,500-L Scale for Monoclonal Antibody Production from Cell Culture. ... studies are needed for assessing cell culture production system options and for testing nutrient supplementation techniques as ... Simpler Noninstrumented Batch and Semicontinuous Cultures Provide Mammalian Cell Kinetic Data Comparable to Continuous and ... Elucidation of Metabolism in Hybridoma Cells Grown in Fed-Batch Culture by Genome-Scale Modeling. Biotechnol. Bioeng. 102:1494- ...
To weaken the Crabtree effect in fed-batch and continuous culture, sugar flow should be limited. In addition, in continuous ... In addition, in continuous culture, the dilution rate must be reduced to avoid washing out cells. However, under such ... To weaken the Crabtree effect in fed-batch and continuous culture, sugar flow should be limited. In addition, in continuous ... To weaken the Crabtree effect in fed-batch and continuous culture, sugar flow should be limited. In addition, in continuous ...
Demonstrates a novel seed-train cell culture process involving high-density cell banking, single-use technology, and perfusion ... Batch Seed-Train Culture in 2-L and 20-L Bags: We thawed an HD cell-bank vial (10 × 107 viable cells/mL, 4.5 mL/vial) into a 2- ... Potential of Cell Retention Techniques for Large-Scale High-Density Perfusion Culture of Suspended Mammalian Cells. Biotechnol ... For a 21-day fed-batch cell culture process using a 500-L bioreactor, an inoculation density of 5.0 × 106 viable cells/mL could ...
Fertile eggs have been created from mouse skin cells for the first time, raising the prospect of new fertility treatments and ... "The idea that you can start with a skin cell and make viable eggs in culture is quite amazing." He hopes the technique can help ... The fertilised eggs were then implanted in the uteruses of albino female mice in batches of around 35 per mouse. Of the 1348 ... Using a well-established technique, the researchers turned these cells into induced pluripotent stem cells - cells that can ...
A recycle system is preferably used to recycle medium containing nutrients for the culture microbes. Byproducts of the cells ... The reaction chamber has an inwardly diverging zone with a velocity gradient which captures cells of varying sizes. ... Bioprocessing apparatus and method using centrifugal acceleration of cells to maintain a culture within a reaction zone. The ... Batch culturing in an incubator typically results in cell suspensions of 104 to 105 cells per ml and antibody concentration ...
The penicillium cells are grown using a technique called fed-batch culture, this way the cells are constantly subject to stress ... Gram-positive bacteria are called protoplasts when they lose their cell wall. Gram-negative bacteria do not lose their cell ... He grew a pure culture of the mould and discovered that it was a Penicillium mould, now known to be Penicillium notatum. ... Fleming coined the term "penicillin" to describe the filtrate of a broth culture of the Penicillium mould. Even in these early ...
... are one of the easiest primary cell cultures to generate. The beauty lies in the fact that in the end you ... The tools and techniques - Plates and pipettes. Sterile plastic petri plates work well for culturing BMDCs. Matrix coated ... If you plan to work on DCs for a long time, it is wise to invest in buying numerous bottles of the same batch or you can ask ... The environment - Cell density and culture medium. The rule of thumb for cell cultures is 1 - 2 million cells per ml. DCs do ...
Batch Cell Culture Techniques Medicine & Life Sciences * Lac Operon Medicine & Life Sciences ... stpA is transiently induced during growth of a batch culture in rich medium. It can also be induced strongly by osmotic shock ... stpA is transiently induced during growth of a batch culture in rich medium. It can also be induced strongly by osmotic shock ... stpA is transiently induced during growth of a batch culture in rich medium. It can also be induced strongly by osmotic shock ...
The Penicillium cells are grown using a technique called fed-batch culture, in which the cells are constantly subject to stress ... This weakens the cell wall of the bacterium, and osmotic pressure becomes increasingly uncompensated-eventually causing cell ... Gram-positive bacteria are called protoplasts when they lose their cell walls. Gram-negative bacteria do not lose their cell ... Bacteria constantly remodel their peptidoglycan cell walls, simultaneously building and breaking down portions of the cell wall ...
... one egg-based and one cell culture-based, had similar efficacy during the 2007-2008 flu season, a researcher said here. ... its obviously very easy then just to discard the current vaccine and start a new batch." In addition, cell culture-based ... are important because public health officials see many advantages to the relatively new cell culture manufacturing techniques. ... "The beauty, if you will, of the cell culture-based vaccine is that it does not have the long lead-in time that we see with egg ...
Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb Cell ... Adivitiya, Dagar VK, Devi N, Khasa YP (2016) High level production of active streptokinase in Pichia pastoris fed-batch culture ... Goyal D, Sahni G, Sahoo DK (2009) Enhanced production of recombinant streptokinase in Escherichia coli using fed-batch culture ... High level production and purification of human interferon α2b in high cell density culture of Pichia pastoris. Enzym Microb ...
Europes leading Cell Culture and Downstream Processing conference for upstream and downstream manufacture ... Comparison of different batches of originator and biosimilar DP • Studies of formulation interactions with human plasma and ... Using a range of analytical techniques, the formation of protein aggregates by various mechanism has been assessed at low and ... Cell Culture & Downstream World Congress 2017. 21 - 22 February. Sofitel Munich Bayerpost, Munich ...
Batch Cell Culture Techniques 100% * fructooligosaccharide 94% * Salmonella typhimurium 82% * Fermentation 76% ... fermentation of carbohydrates by porcine faecal inocula and their influence on Salmonella Typhimurium growth in batch culture ... fermentation of carbohydrates by porcine faecal inocula and their influence on Salmonella Typhimurium growth in batch culture ...
  • An optimum range of agitation speeds in the 800-1000 rpm range corresponding to Reynolds numbers of 50000-63000 (based on initial batch conditions) seemed to give the best balance between substrate utilization for biomass growth and citric acid production. (
  • In this study, several models were developed to describe biomass, nisin, and lactic acid production as well as sucrose consumption during nisin fermentation by L. lactis in suspended-cell batch culture. (
  • Several techniques have evolved to model biomass statistically, however, there are problems with model transfer between cell lines and process conditions. (
  • A simple linear factor was found to be responsible for the transferability of biomass models between cell lines, indicating a link to their phenotype or physiology. (
  • Herwig, C. Universal Capacitance Model for Real-Time Biomass in Cell Culture. (
  • We used a metabolomics analysis to identify metabolites that were drastically increased or decreased in continuous culture when the dilution rate shifted from biomass formation to ethanol fermentation. (
  • Custom-developed software rapidly measures light transmission and subsequently adjusts the irradiance to maintain a defined light profile to compensate for culture dynamics, biomass accumulation, and pigment adaptations during physiological transitions, thus ensuring appropriate illumination across batch and continuous growth modes. (
  • Various key variables (biomass, substrate and product) of bioprocesses should be monitored in order to retrieve useful information on the system, with the biomass (the cell density) the principal target. (
  • In the present work, noninduced glycerol batch cultures (for which biomass and substrate are the key variables) were monitored using multiwavelength fluorescence spectroscopy. (
  • BACKGROUND To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary phase where cellular energies are directed towards production of rP. (
  • Plant cell suspensions are frequently considered to be heterogeneous with respect to growth in terms of progression of the cells through the cell cycle and biomass accumulation. (
  • After adding BrdU to test Harpagophytum procumbens cell suspension cultures the cell cycle distribution could be adequately resolved using its incorporation for the following 72h (after which BrdU slowed biomass accumulation). (
  • When applied for production of a fusion protein, the TLFB technique resulted in higher cell density, lower cell death, higher concentration of the product and drastically lower proteolytic degradation of the recombinant protein compared to corresponding methanol limited P. pastoris bioreactor cultures. (
  • Finally, it was demonstrated that viral infection of HeLa cells grown in 4-L bioreactor batch cultures resulted in increased death and glucose uptake rates and significantly lower growth rates. (
  • Trainees will prepare and run a steam in place cycle on a stainless steel bioreactor and then inoculate a production batch into a stainless steel bioreactor. (
  • When culture volume and cell density meet predetermined criteria, the culture is transferred to a production bioreactor in which cells continue to grow and express product. (
  • the new process uses high-density (HD) cell banking, single-use technology, and high-density perfusion at the n - 1 stage to allow for high-density inoculation in the production bioreactor. (
  • HD perfusion at the n - 1 stage allows for HD inoculation of the production bioreactor, reducing the time to steady-state cell density by 4-5 days and increasing productivity by 10% for a 50-day run. (
  • It may take even longer and become more complex when production bioreactors are very large because additional large-volume culture vessels are required to support scale up to a production bioreactor. (
  • combined HD cell banks with a single seed bioreactor capable of operating at several working volumes to reduce seed-train expansion duration by 60-70% ( 3 ). (
  • Perfusion processes can reach high cell densities in relatively small bioreactor volumes ( 10 ) and remove unstable product from cultures more quickly ( 11 ) than batch and fed-batch processes. (
  • And sparging can also cause significant foaming at the surface of the bioreactor, which may also decrease the number of viable cells, although various detergent-like solutions are available to reduce the effect. (
  • Our new technology also offers complete control over the rate of cell production, so it could be scaled up using existing stacked culture flasks to produce one billion cells per week, or scaled down so as to fit a bioreactor on the head of a pin. (
  • Rice oil significantly affected cephalosporin C production in a 2.5-l bioreactor culture of Cephalosporium acremonium M25. (
  • Perfusion involves the constant removal of cell-culture fluid from a bioreactor, while adding fresh media. (
  • Again, if a batch from one bioreactor is contaminated, the pooled batches will be lost. (
  • Primary cells, finite cultures, and continuous cell lines differ in their proliferative potential (see below). (
  • Primary cell culture is generally more difficult than culture of continuous cell lines. (
  • Primary cell cultures are sometimes preferred over continuous cell lines in experimental systems. (
  • Furthermore, many cell types are not available as continuous cell lines. (
  • The phenotype of these cultures is intermediate between finite cultures and continuous cultures. (
  • Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. (
  • Rodent primary cell cultures form continuous cell lines relatively easily, either spontaneously or following exposure to a mutagenic agent. (
  • In contrast, human primary cell cultures rarely, if ever, become immortal in this way and require additional genetic manipulation to form a continuous cell line. (
  • Effect of Cell Culture Process Change from Fed-batch to Continuous on. (
  • To convert an existing fed-batch cell culture process to continuous, or perfusion, the major technical hurdles include maintaining steady state cell culture performance and generating product with comparable product quality attributes. (
  • used logistic fit of simpler noninstrumented batch and semicontinuous cultures to compare cell growth kinetics, nutrient consumption, metabolite, and product formation data with those of more complex continuous and perfusion models ( 3 ). (
  • To weaken the Crabtree effect in fed-batch and continuous culture, sugar flow should be limited. (
  • In addition, in continuous culture, the dilution rate must be reduced to avoid washing out cells. (
  • Therefore, the purpose of this study was to reduce ethanol production in continuous culture of S. cerevisiae at a higher dilution rate through a metabolomics approach. (
  • This result demonstrates the potential for using metabolomics approaches to identify metabolites that reduce ethanol production in continuous culture at high dilution rates. (
  • A technique which revolutionises cell culture by allowing the continuous production and collection of cells, has been developed by scientists at Newcastle University. (
  • Remarkably, with this continuous production technique even a culture surface the size of a penny can, over a period of time, generate the same number of cells as a much larger-sized flask. (
  • The self-detaching cells are then produced in a continuous bioprocess and available for use in a variety of downstream applications without losing their original characteristics. (
  • The potential reduced size of a continuous cell bioprocess has obvious advantages in terms of lower production costs and increased coverage and application. (
  • Martina Miotto, a PhD student from the Institute of Genetic Medicine, who is first author on the paper, said: "The concept of a continuous bioprocess is currently used to produce biopharmaceuticals such as vaccines and anti-cancer antibodies, but never before for cells. (
  • Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture. (
  • Xylose and glucose utilization by Bacteroides xylanolyticus X5-1 cells grown in batch and continuous culture. (
  • Xylose-limited continuous-culture cells were able to consume xylose and glucose both as single substrates and as mixed substrates without any lag phase. (
  • The inability of B. xylanolyticus X5-1 cells undergoing glucose-limited continuous culture to induce the xylose catabolic pathway after the addition of only xylose probably was caused by energy limitation. (
  • Fingerprint Dive into the research topics of 'Xylose and glucose utilization by Bacteroides xylanolyticus X5-1 cells grown in batch and continuous culture. (
  • abstract = "{\textcopyright} 2019 Elsevier B.V. In this work, continuous and fed-batch operational modes for producing the recombinant protein lipase B from Candida antarctica under the new constitutive promoter PGK in the cell factory Pichia pastoris was assessed. (
  • Thus, qp was linearly related in the continuous mode but suggested saturation in fed-batch cultures. (
  • The highest volumetric and specific productivity were obtained at the highest μlevels in the continuous mode, which provided volumetric and specific productivities (QP and Qe, respectively) roughly 1.5 and 3 times greater than the fed-batch mode. (
  • Continuous operation with the novel constitutive promoter PGK is thus a realistic alternative to the standard fed-batch cultivation at a high cell density typically used in industrial bioprocesses for heterologous protein production in Pichia pastoris. (
  • These stable cell lines retain 95-100% of productivity after 40 days of continuous culture (~48-56 generations). (
  • Among methods increasingly being adopted is perfusion cell culture (also called upstream continuous culture). (
  • This continuous process produces higher yielding products with a better consistency, compared with fed-batch processing and other batch processing techniques. (
  • Through insight into the advancement of continuous processing techniques, you will explore how new technology and production techniques can enable you to meet regulatory and quality assurance challenges whilst simplifying the management of your supply chain. (
  • Among the propositions was the idea that their chick heart cultures could live indefinitely by continuous application of their methods. (
  • When a finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes a continuous cell line . (
  • A typical cell culture process begins with thawing of a cryopreserved cell-bank vial, followed by successive expansions into larger culture vessels such as shake flasks, spinners, Wave bags, and stirred bioreactors ( 1 ). (
  • As cultures increase in cell concentration and volume during scale-up into bioreactors, not only are nutrients depleted, but waste products can accumulate to sufficient levels to limit product formation. (
  • Oxygenation limitations can become significant in very high-density cultures (as in hollow-fiber bioreactors) even where bubble sheer is not an issue. (
  • A similar problem arises at the beginning of the purification process, in which batches from several bioreactors are fed into a single purification step. (
  • HD cell banking and disposables improve operational success by reducing seed-train complexity and contamination potential. (
  • Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts - MEFs). (
  • Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. (
  • This video is focused on the steps you should take to prevent contamination of your cell culture. (
  • Using proper technique is imperative for avoiding cross-contamination that can ruin the sample and affect the entire cell culture process. (
  • Cell immobilization techniques have become more important and popular because it gives many advantages compared to cell suspension culture such as maintenance of stable and active biocatalysts, accelerated reaction rates, high volumetric productivity, improved process control, reduced susceptibility of cells to contamination, improved production efficiency and no possibility of washout. (
  • This problem is often mentioned in connection to standard methanol limited fed-batch P. pastoris processes [ 1 ]. (
  • However, as compared to fed-batch processes, product quality and cell specific productivity differences were observed in perfusion cultures. (
  • This presentation provides an insight into the product quality and productivity differences between traditional fed-batch and perfusion cell culture processes and potential approaches to addressing these differences. (
  • Previous efforts have shown that high-density (HD) cell banking can be an effective means to reduce the number of seed-train steps required and also improve operational success in seed-train processes. (
  • Industry interest in perfusion cell-culture processes has been increasing ( 8 ). (
  • The commercial availability of steam-sterilizable dielectricspectroscopy probes has enabled successful adoption of this technology as a key noninvasive method to measure VCV for cell-culture processes. (
  • Transfection is routinely used in gene editing and gene silencing techniques that have enhanced our understanding of complex biological processes and enabled the use of gene therapy to treat disease. (
  • The fermentation by conventional methods that use cell suspension in batch processes has several limitations, such as low productivity, product inhibition and also the variation from batch to batch. (
  • By combining Fujifilm's experience gleaned from the intricate process of manufacturing photographic film along with FCDI's knowledge of cell reprogramming, genetic engineering and cell differentiation, the facility is poised to address the complex manufacturing processes of cell therapies. (
  • The adhesion of Plasmodium falciparum parasitized red blood cell (PRBC) to human endothelial cells (EC) induces inflammatory processes, coagulation cascades, oxidative stress and apoptosis. (
  • Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. (
  • The majority of biopharmaceutical processes use a fed-batch nutrient-feeding regime to maintain cell culture activity. (
  • Current fed-batch processes rely on predetermined feeding protocols based on nutrient requirement estimates or infrequent sampling and offline measurement of the culture media to determine the concentration of key components. (
  • Anything that had to do with the growth of cells, their structure, life-cycle, even their development within a tissue, was all related to cancer, because cancer represented the case when these processes started to go wrong. (
  • Fed-batch production of citric acid by Candida lipolytica grown on n-paraffins. (
  • Hematopoietic cells (derived from blood, spleen, or bone marrow) as well as some transformed cell lines and cells derived from malignant tumors can be grown in suspension. (
  • Moreover, when cells are grown in minimal medium, we observe a more sustained induction of stpA which is dependent on the leucine-responsive regulatory protein (Lrp). (
  • The process removes the limit on the number of cells that can be grown in a culture dish, which until now has been strictly confined by its surface area. (
  • The research published today in ACS Applied Materials & Interfaces reports how the Newcastle team have developed a coating that allows individual stromal cells to "peel away" from the surface on which they are grown. (
  • Traditionally, cells have been grown in the lab over the surface area of a flask and then detached chemically or enzymatically for use. (
  • The cells are created in batches with batch size limited to the area upon which the cells are grown. (
  • Xylose uptake in glucose-grown cells was very low, but the uptake rate could be increased when incubated with a xylose-glucose mixture. (
  • Most cells are anchorage-dependent and must be cultured while attached to a solid or semi-solid substrate ( adherent or monolayer culture ), while others can be grown floating in the culture medium ( suspension culture ). (
  • Lymphoblast-like cells are spherical in shape and usually grown in suspension without attaching to a surface. (
  • Common formats to induce ESC differentiation in vitro include monolayer culture on defined matrices, 15 co-culture with heterotypic cell types 16 and the formation of cell aggregates grown in suspension termed embryoid bodies (EBs). (
  • Cell-based vaccines are grown in cell cultures, unlike most flu vaccines, which are made inside fertilized chicken eggs, a slower technique that dates to the 1930s. (
  • Due to its short generation time (∼90 min) and modest culturing requirements, yeast can also be grown rapidly in high-throughput experimental setups. (
  • Unfortunately, due to secretion of proteases to the medium, and possibly also due to release by lysis, proteolytic degradation is a significant problem in many high cell density cultures. (
  • This work describes the use of an alternative fed-batch technique, the temperature limited fed-batch (TLFB), in which the common methanol limitation is replaced by temperature limitation, in order to avoid oxygen limitation at high cell density. (
  • Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process. (
  • Steady state culture at high cell density was achieved for all three molecules, which allows the delivery of products with consistent product quality and adequate productivity. (
  • Ayed A, Rabhi I, Dellagi K, Kallel H (2008) High level production and purification of human interferon α2b in high cell density culture of Pichia pastoris . (
  • Here we describe the application of a closed-loop control scheme for the long-term cultivation of CHO cells in a high cell density (35 - 40 million cells/ml) perfusion process. (
  • Primary cell cultures come from the outgrowth of migrating cells from a piece of tissue or from tissue that is disaggregated by enzymatic, chemical, or mechanical methods. (
  • Primary cells are morphologically similar to the parent tissue. (
  • Virtual tissues services for biomedical research features high fidelity tissue simulation and modelling to grow virtual tissue from a single cell, giving unprecedented insight into lineage commitment phenomena of stem cells with insight i. (
  • But to fully mature, the cells needed help, says Hayashi - tissue taken from the ovaries of mouse fetuses. (
  • One issue is that Hayashi's team used fetal tissue in the experiment to give the egg cells the final push to maturity. (
  • It's possible that to do the same with human cells, tissue from aborted fetuses could be used, but researchers are likely to need to develop an alternative method. (
  • Che Connon , Professor of Tissue Engineering and author of the paper, said: "This allows us to move away, for the first time, from the batch production of cells to an unremitting process. (
  • Pipette 250 uL of cells into each T25 tissue culture flask. (
  • Karantalis and Hare [ 4 ] summarized the biology of mesenchymal stem cells and reviewed their wide utilization in cardiac tissue repair and regeneration therapy. (
  • The characterization of regulatory T cells in individuals with advanced HIV-1 disease or tissue samples, for which only very small biological samples can be obtained, is therefore extremely challenging. (
  • Split the cells 1:10 in 10 mL culture medium (without G418 if conditioned medium is to be used with a cell line sensitive to G418) in 10 cm tissue culture dishes or T-75 flasks and let the cells grow for 4 days (approximately to confluency). (
  • Plant tissue culture 1. (
  • Plant Tissue Culture is way to grow whole plant from any part in vivo. (
  • Plant Tissue Culture Techniques are two types like batch and static cultures. (
  • Plant Tissue Culture - authorSTREAM Presentation. (
  • Along with Dr. Alexis Carrel, a surgeon at Rockefeller Institute (1906 - 1927), Dr. Burrows is credited with coining the phrase "tissue culture", and is among the first to adapt such methods to the study of tissues from warm-blooded animals. (
  • In 1910 during a visit to the laboratory of Ross Granville Harrison at Yale, Burrows studied tissue culture. (
  • At Yale, Burrows also successfully established tissue cultures of embryonic chick cells. (
  • These improvements include the substitution of blood plasma for lymph and the addition of embryo extract in the tissue culture medium that improved the growth of tissue cultures. (
  • Because the tissue cultures far outlived their organisms, they were deemed "immortal. (
  • Burrows and Carrel went on to expand the application of their tissue culture techniques to additional types of tissues including: adult tissues, tissues of mammalian origin, and cancerous tissues from chicks and humans. (
  • PMLTEST412A - Prepare tissue and cell cultures. (
  • The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established. (
  • Primary culture refers to the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate (i.e., reach confluence ). (
  • 13 , 14 Similarly, ESCs injected into various tissue sites of adult organisms spontaneously form teratomas, benign tumors composed of a disorganized mix of cells from all three germ layers. (
  • Leveraging technology that can be used to create induced pluripotent stem cells (iPSCs) and differentiated tissue-specific cells from any individual, FCDI is committed to advancing life science research and transforming the therapeutic development process in order to fundamentally improve human health. (
  • This report analyzes the worldwide markets for Cell and Tissue Culture Supplies in US$ Thousands by the following Product Segments: Media, Sera, and Reagents. (
  • Previous studies to evaluate the developmental potential of candidate hESC-derived progenitors have delivered these cells into murine and porcine cardiac tissue, with inconclusive evidence regarding the capacity of these human cells to physiologically engraft in xenotransplantation assays. (
  • a technique for double in situ hybridization to simultaneously detect porcine circovirus 2 (pcv2) and porcine parvovirus (ppv) in the same tissue section was developed and applied to lymph node and spleen from 8 pigs experimentally coinfected with pcv2 and ppv and 20 pigs with naturally occurring postweaning multisystemic wasting syndrome. (
  • In 1885, Wilhelm Roux successfully maintained a portion of an embryonic chicken, in warm saline water, establishing the principle of tissue culture. (
  • An English physiologist Sydney Ringer, developed salt solutions containing essential elements, for keeping live cells active outside the mother tissue. (
  • The concept of maintaining live cells outside the original tissue was discovered in the nineteenth century. (
  • Accordingly, depletion of ELOVL6 in parental MM cells suppressed BTZ-induced endoplasmic reticulum (ER) stress and cytotoxicity, whereas restoration of ELOVL6 levels in BTZ-resistant MM cells sensitized them to BTZ in tissue culture settings and, as xenografts, in a plasmacytoma mouse model. (
  • Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. (
  • For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques. (
  • Temperature-sensitive miR-483 is a conserved regulator of recombinant protein and viral vector production in mammalian cells. (
  • In this study, we make use of the well-known pore-forming bacterial toxin streptolysin O (SLO) to label intracellular proteins in mammalian cells for fluorescence microscopy applications. (
  • For cultures to last the maximum amount of time, you also need a refrigerator that will not share any food products with the stored plates (mold & bacteria risk otherwise) and be kept at about 35 degrees. (
  • A growth curve was plotted for strain GR-1, and adherence was found to be lowest for bacteria in early log phase (39 bacteria per uroepithelial cell) and highest in stationary phase (59 bacteria per uroepithelial cell). (
  • These experiments illustrate that growth phase and culture conditions affect the extracellular structure of lactobacilli and also affect the adherence capacity of these bacteria. (
  • The complexity of a biological system can be resolved by using its physicochemical properties to isolate and separate its specific constituent bio-organisms and -molecules, such as cells, bacteria, proteins, and DNA. (
  • The aim of this project is to optimize the performance of long-term cultures of lactic acid bacteria in a fixed-bed reactor system. (
  • The Bacillaceae family members are a good source of bacteria for bioprocessing and biotransformation involving whole cells or enzymes. (
  • CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. (
  • In the TLFB process, cell death rate was low and constant and reached 4% dead cells at the end of cultivation (about 150 hours methanol feeding time). (
  • Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest. (
  • In addition to chemostat cultivation, the photobioreactor may also operate as a turbidostat, continuously adjusting the media dilution to achieve maximal growth at a fixed culture density. (
  • The biotechnological qualities of yeast cells depend on their metabolic state, cultivation conditions and on their capacity to adapt to different stress factors. (
  • Using a combinatorial materials approach we develop a synthetic, chemically defined cellular niche for the support of functional cardiomyocytes derived from human embryonic stem cells (hESC-CMs) in a serum-free fully defined culture system. (
  • The chemical moieties identified in this large-scale screen provide chemically defined conditions for the culture and manipulation of hESC-CMs, as well as a framework for the rational design of superior biomaterials. (
  • Results: In this report, retrovectors were packaged in CHO cells cultured in chemically defined (CD) media. (
  • However, some cell lines become immortal through a process called transformation , which can occur spontaneously or can be chemically or virally induced. (
  • The quest for increased productivity and better process control combined with patient safety has encouraged biopharmaceutical companies to use chemically defined media for cell culture. (
  • Especially insights that would otherwise be difficult or impossible to obtain with macroscopic cell culture in traditional polystyrene dishes, flasks or well-plates. (
  • During this course trainees will be introduced to cell culturing techniques using small scale shake flasks. (
  • In liquid media, hyphae were found sparsely and only in the wash-zone on walls of the culture flasks. (
  • CGTase activity produced from Bacillus circulans DF 9R was optimised in shake flasks using a combination of conventional sequential techniques and statistical experimental design. (
  • Application of multiple fed-batch feedings can be used to extend the batch fermentation and increase final citric acid concentrations and product yield. (
  • Increased citric acid concentrations in three-cycle fed-batch mode was achieved at longer fermentation times. (
  • This aerobic ethanol fermentation, referred to as the Crabtree effect, is one of the most significant reasons for low cell yield. (
  • Chromatography remains the main technique for purifying biomolecules from cell culture supernatant of mammalian or bacterial fermentation. (
  • The reason it is required to know cell counts is to ensure you are supplying the right amount of yeast for a healty fermentation (neither too high or too low) and the assessment methods to determine the amount of viable vs. dead cells so that you can correct the pitching amount to take that into account. (
  • Media compositions used for cultivating recombinant cell lines not only directly influence the cells' physiological phenotype and fermentation performance, and quality of the expressed product, but the development of novel media formulations also provide a mechanism of generating manufacturing intellectual property for a particular product. (
  • Although several analytical methods have been adapted and used to monitor the evolution of cell density evolution in cultures, a general method for performing this determination has not yet been established, as each technique has its own advantages and drawbacks. (
  • We are exploring the use of rapid 'holistic' analytical methods that can be used for routine screening of cell culture media used in industrial biotechnology. (
  • Yeast phase growth (blastospores) occurred in pH- buffered media that were nutrient balanced, or continuously carbon-limited (fed-batch culture), or carbon-exhausted (batch culture). (
  • Yeast Cultures. (
  • Although an old thread did provide a little help, I still have a few questions about reusing my yeast culture. (
  • I would be happy to learn of someone who has kept a yeast culture going (on the homebrew level) for anything over 6 months. (
  • I have been contemplating yeast culturing for some time now, but I still have quite a few other purchases to finish off my home-brewery before I take that step. (
  • After I see how I faire at hop growing I was planning on progressing to yeast culturing. (
  • There is a GREAT book available that describes IN ENGLISH how to culture yeast on a homebrew scale by Pierre Rajotte or there is a more pamphlet like book by Rog Leistad called Yeast Culturing for the Homebrewer. (
  • The first book, however, gets into yeast assessment and cell counting which, if you are serious about the topic, is required. (
  • The latter formed a long, relatively dense, fibrous capsule after growth in brain heart infusion yeast extract agar, unlike strains GR-1 and RC-17, which formed a short, tightly bound, electron-dense capsule which surrounded the cells in a radial fashion. (
  • The data gathered were modelled via PARAFAC-PLS chemometric methodologies, resulting in important qualitative and quantitative information about the behaviours of different biogenic fluorophors in batch cultures of the yeast Pichia pastoris. (
  • Unfortunately, within a few days, Barry notices that in his new batch, the yeast don t appear to be growing in one fermenter, and no gas is being produced. (
  • Students will use problem-solving skill and cell biology lab techniques to examine yeast cultures provided by the instructors. (
  • abstract = "The influence of various culture parameters on infection and replication of recombinant vaccinia virus in HeLa cells was examined during various phases of viral replication. (
  • Mice that are injured, unhealthy or immune-compromised show effects on all immune cells, including DC numbers and differentiation. (
  • Human pluripotent stem cells (hPSCs) are clonogenic cells capable of self-renewal and multilineage differentiation. (
  • 8-12 The "induced" pluripotent stem (iPS) cells created from both mouse and human somatic cells appear similar to ESCs in terms of both self-renewal and differentiation capacity. (
  • Blastocyst injection and teratoma studies demonstrate that the environment into which pluripotent cells are introduced can influence differentiation, however, in vivo studies are limited in their ability to attain mechanistic insights into the effects of environmental factors on stem cell differentiation. (
  • In contrast, differentiation of ESCs in vitro, affords more controlled methods to present morphogenic cues in the stem cell microenvironment and directly assess differentiated cell phenotypes. (
  • The in vitro culture of ESCs as EBs affords opportunities to mechanistically study early differentiation events of 3D assemblies of pluripotent cells. (
  • One advantage of in vitro differentiation studies is that genetic manipulation of ES cells can be studied for gene mutations or knockouts that prove to be lethal during normal embryonic development in vivo. (
  • 24 EB differentiation begins with the formation of an aggregate of ESCs, the size of which is dependent on the number of cells which initially self-assemble via cell-cell adhesion receptors. (
  • Corning Matrigel Matrix is a reconstituted basement membrane preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors and is widely used for a variety of applications such as general cell culture, in vivo study, hESC or organoid culture and differentiation. (
  • Existing methods for human induced pluripotent stem cell (hiPSC) cardiac differentiation are efficient but require complex, undefined medium constituents that hinder further elucidation of the molecular mechanisms of cardiomyogenesis. (
  • Along with small molecule-based induction of differentiation, this protocol produced contractile sheets of up to 95% TNNT2(+) cardiomyocytes at a yield of up to 100 cardiomyocytes for every input pluripotent cell and was effective in 11 hiPSC lines tested. (
  • A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut + strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. (
  • Adivitiya, Dagar VK, Devi N, Khasa YP (2016) High level production of active streptokinase in Pichia pastoris fed-batch culture. (
  • This attachment is essential for proliferation - many adherent cell cultures will cease proliferating once they become confluent (i.e., when they completely cover the surface of cell culture vessel), and some will die if they are left in this confluent state for too long. (
  • Hence several studies have been performed to generate and expand highly cytotoxic NK cells ex vivo , e.g., by using specific cytokines to upregulate both their proliferation and surface expression of distinct activating receptors. (
  • DPSCs were characterized first, followed by cell proliferation, pluripotency, and migration study in SCM and E8 medium. (
  • Compared with serum-containing medium, E8 medium exhitibed higher ability in maintaining the cell proliferation, pluripotency, migration, and stability. (
  • With our new technology, one square meter would produce enough cells to treat 4,000 patients, while traditional methods would require an area equivalent to a football pitch! (
  • Chemical, physical, and biological methods are used to transfect cells, enabling the study of gene function and expression within a cellular environment. (
  • Upon returning to Carrel's laboratory Burrows initiated Harrison's methods and went on to make significant improvements to Harrison's original techniques. (
  • There are a variety of analytical methods available for characterization at the intact protein level, including chromatography, electrophoresis, and mass spectrometry (MS). Recent advances in capillary electrophoresis (CE) techniques have been used for detailed characterization of glycans attached to biotherapeutic proteins. (
  • The threat of newly emerging pandemic influenza strains, and limitations of egg-based culture, has driven the need for rapid response manufacturing methods. (
  • Disclosed herein are methods for culturing primate embryonic stem cells. (
  • The present invention relates to methods for culturing primate embryonic stem cell cultures and culture media useful therewith. (
  • In recent years, a market surge in biopharmaceuticals has warranted extensive research and development in advanced cell culturing techniques and subsequent methods of optimizing bioprocesses. (
  • We have developed robust methods which can be used to identify compositional changes and ultimately predict the efficacy of individual fed batch media in terms of downstream protein product yield with an accuracy of ±0.13 g/L. This is achieved through the implementation of chemometric methods such as multiway robust principal component analysis (MROBPCA), and n-way partial least-squares-discriminant analysis and regression (NPLS-DA and NPLS). (
  • Despite this limitation, the protocol allows resolution of the cell cycle distribution of cultures that cannot be analyzed using commonly applied methods due to the cells' fragility. (
  • Sparging with air or oxygen to maintain appropriate O 2 levels can become problematic because of excessive cell sheer, which may lower growth rates or decrease viability. (
  • For some cell lines, the technique's accuracy in predicting the VCV from probepermittivity measurements declines as the viability of the cell culture decreases. (
  • Wyeth evaluated and used a viability-based technology, solid-phase cytometry, in mammalian cell-culture manufacturing. (
  • Viability-based technologies use techniques that are linked to the viability of the microbial cells or the potential for the cells to grow. (
  • All of these techniques require a high powered microscope, a hemocytometer (counting cell) and viability stains in addition to culture tubes, agar medias, pyrex plates and other supportive equipment. (
  • A variety of cell viability tests were performed after treatment with SLO to ensure that the cells have intact membranes, are able to divide, respond normally to signaling molecules, and maintains healthy organelle morphology. (
  • Second, drug candidates that exhibit cytotoxicity by off-target effects, or a different response of the desired target in vivo upon ligand binding, can easily be ruled out by monitoring cell viability. (
  • The common substrate limited fed-batch (SLFB) technique utilizes the fact that the oxygen consumption rate is approximately proportional to the substrate feed rate. (
  • A: Characteristics of a substrate limited fed-batch technique with constant temperature. (
  • Soluble components of the culture system such as growth factors within serum and insoluble components such as the substrate on which cells adhere to are important variables controlling the biological activity of cells. (
  • Fibroblastic (or fibroblast-like) cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate. (
  • Epithelial-like cells are polygonal in shape with more regular dimensions, and grow attached to a substrate in discrete patches. (
  • 3 Culture of ESCs in planar formats (i.e. monolayer, co-culture) attempt to provide a more defined substrate for ESC attachment and uniform exposure to soluble media components, whereas the 3D aggregates of ESCs formed by EB culture techniques more accurately recapitulate the complex assembly of cell adhesions and intercellular signaling of early embryogenesis. (
  • The most promising 3 polymers were scaled up for extended culture of hESC-CMs for 15 days and were characterized using patch clamp electrophysiology and myofibril analysis to find that functional and structural phenotype was maintained on these synthetic substrates without the need for coating with extracellular matrix protein. (
  • With this method, we generated glucagon-like protein-1 Fc fusion protein (GLP-1-Fc) stable expression CHO cell lines. (
  • Retrovectors packaged from this system could be used to generate CHO stable cell lines for recombinant protein expression. (
  • Cells can be stably transfected for the integration of DNA into their genome, or transiently transfected for protein expression of temporary duration. (
  • Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development. (
  • The cells secrete biologically active Wnt-3A protein. (
  • Although both cell lines quickly produced effective VLPs the researchers believe BTI-TN5B1-4 has advantages over Sf9, in part because it decreases protein and baculovirus background. (
  • Medium-throughput production of recombinant human proteins: protein production in insect cells. (
  • Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures. (
  • Some of these techniques include cell transformation, use of agar plates, batch cell culture, inducing protein expression, and protein purification and analysis. (
  • Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition. (
  • Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design. (
  • Influence of low temperature on productivity, proteome and protein phosphorylation of CHO cells. (
  • Example applications in conventional and super-resolution imaging of native and transfected cells include p65 signal transduction activation, single molecule tracking of kinesin, and specific labeling of a series of nuclear and cytoplasmic protein complexes. (
  • Another method to overcome the permeability issue is by transfecting the cells with plasmid DNA encoding the intracellular protein of interest appended to a fluorescent protein (FP). (
  • First, we permeabilized the cells with SLO at 37°C for 7-10 min depending on the cell confluency, followed by incubation with the fluorescent probes (i.e., fluorophores by themselves, or covalently attached to a ligand or a protein) for 5 min on ice. (
  • Moving in vitro culture from macroscopic culture to PDMS based devices can come with unforeseen challenges. (
  • Cardiomyocytes from human stem cells have applications in regenerative medicine and can provide models for heart disease and toxicity screening. (
  • The potential utility of increased structural integrity was demonstrated in an in vitro toxicity assay that found an increase in detection sensitivity of myofibril disruption by the anti-cancer drug doxorubicin at a concentration of 0.05 μM in cardiomyocytes cultured on the co-polymer compared to 0.5 μM on gelatin. (
  • Here, we have prospectively identified a population of hESC-derived ROR2(+)/CD13(+)/KDR(+)/PDGFRα(+) cells that give rise to cardiomyocytes, endothelial cells, and vascular smooth muscle cells in vitro at a clonal level. (
  • Here, we use primate pluripotent stem-cell-derived cardiomyocytes that mimic fetal cardiomyocytes in vitro to discover hundreds of ERV transcripts from the primate-specific MER41 family, some of which are regulated by the cardiogenic transcription factor TBX5. (
  • The precise constitution of the penicillin extracted depends on the species of Penicillium mould used and on the nutrient media used to culture the mould. (
  • Scale-up studies are needed for assessing cell culture production system options and for testing nutrient supplementation techniques as well. (
  • One published fed-batch scale-up study testing from 3 L up to 2,500 L highlights items to be considered in addition to the nutrient supplementation process such as the impact of pH and CO 2 control ( 1 ). (
  • Modeling has proven to be successful in defining appropriate nutrient supplementation schemes, especially when it's validated through a limited number of scale-up cultures. (
  • Improvement of ethanol production efficiency from sweet sorghum juice (SSJ) under normal gravity (NG, 160 g/L of sugar), high gravity (HG, 200 and 240 g/L of sugar) and very high gravity (VHG, 280 and 320 g/L of sugar) conditions by nutrient supplementation and alternative feeding regimes (batch and fed-batch systems) was investigated using a highly ethanol-tolerant strain, Saccharomyces cerevisiae NP01. (
  • Success in efficiently performing the complex and interdependent metabolic reactions critically depends upon the precise makeup of the cell culture medium and the nutrient feed regime, which controls the medium composition throughout the duration of the drug production process. (
  • Instead, companies still depend upon predetermined nutrient regimes or periodic extractive sampling and offline analysis, resulting in suboptimal conditions for cell culture, process delays and risk of infection. (
  • Both these techniques can lead to nonoptimal feeding, with depletion of nutrient and large swings in nutrient concentration, risking the health of the organism and its ability to produce consistent product quality at a high concentration. (
  • This ensures the cells are maintained in a steady more-productive state than is the case with larger, more infrequent nutrient swings. (
  • In addition, a freeze substitution technique was developed to examine the morphology of strains Lactobacillus casei ss rhamnosus RC-17, L. casei GR-1, and L. acidophilus T-13 in relation to growth conditions and adhesion. (
  • Cells in culture can be divided into three basic categories based on their shape and appearance (i.e., morphology ). (
  • He hopes the technique can help us understand how eggs develop, and learn more about the effects of genetic mutations on fertility. (
  • The approach could also theoretically be used to create egg cells from male skin cells, raising the prospect of babies with two genetic fathers. (
  • HEK-293 and their genetic derivatives are principal cells for retrovector production. (
  • Calcium phosphate facilitates the binding of DNA to the cell surface, allowing genetic material to enter the cell by endocytosis. (
  • A cell strain often acquires additional genetic changes subsequent to the initiation of the parent line. (
  • Broader Implications From a personal standpoint, completing this work allowed me to gain many skills and learn about techniques used in genetic engineering and cell culture. (
  • Murine bone marrow derived dendritic cells (BMDCs) are one of the easiest primary cell cultures to generate. (
  • ROR2(+)/CD13(+)/KDR(+)/PDGFRα(+) cells were delivered into these functioning fetal heart tissues: in contrast to traditional murine heart models for cell transplantation, we show structural and functional integration of hESC-derived cardiovascular progenitors into human heart. (
  • Using a well-established technique , the researchers turned these cells into induced pluripotent stem cells - cells that can continue to divide, and can form various other types of cell. (
  • The team has also demonstrated that the process works across a range of stromal cells including mesenchymal stem cells (MSCs). (
  • The concept of establishing a dental stem cell (DSC) bank for oral and maxillofacial regeneration has become of great interest but it remains at a primitive stage. (
  • 2 ] presented a CRISPER/Cas9 gene-editing system with homologous recombination at the HBB gene in hematopoietic stem cells and achieved 90% targeted integration of purified hematopoietic stem and progenitor cells. (
  • Stem cells can also be applied for chronically injured organs. (
  • Applications include gene therapy, generation of induced pluripotent stem cells (iPSC), gene silencing by RNA interference (RNAi), and the production of therapeutic antibodies and proteins. (
  • Stem cells and progenitor cells in ischemic stroke - fashion or future? (
  • Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. (
  • Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. (
  • Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. (
  • Previously, groups of the Stem Cell Network North-Rhine Westphalia evaluated the use of GFP-positive pluripotent embryonic stem cells (ESCs) after experimental ischemia and traumatic brain injury (TBI). (
  • Embryonic stem cells (ESCs) are capable of limitless self-renewal in vitro and differentiate into cells constituting all three primitive germ layers-mesoderm, ectoderm and endoderm, as well as germ cells (sperm and ova). (
  • See other webinars in this series on our webinar page - Advanced Trends and Tools for Immune Cell Therapy and Advanced Trends and Tools for Stem Cell Therapy. (
  • January 3 , 2019 -- FUJIFILM Cellular Dynamics, Inc. (FCDI), a US subsidiary of FUJIFILM Corporation (President: Kenji Sukeno) and a leading global developer and manufacturer of human induced pluripotent stem (iPS) cell technologies, has announced an investment of about $21 million to open a new cGMP-compliant *1 production facility with the goal of industrializing iPS cell manufacturing for regenerative medicine therapies. (
  • culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of fibroblast growth factor that is supplied from a source other than just a fibroblast feeder layer. (
  • 6 . The method of claim 2 , wherein the primate embryonic stem cells are human embryonic stem cells. (
  • 7 . The method of claim 2 , wherein said culturing step includes the embryonic stem cells proliferating in culture for over one month while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the karyotype of the stem cells. (
  • culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of a growth factor capable of activating a fibroblast growth factor signaling receptor, wherein the growth factor is supplied from a source other than just a fibroblast feeder layer. (
  • Primate (e.g. monkey and human) pluripotent embryonic stem cells have been derived from preimplantation embryos. (
  • Stem cell reprogramming: A 3D boost. (
  • We conclude that recently evolved TE loci such as BANCR may represent potent de novo developmental regulatory elements that can be interrogated with species-matching pluripotent stem cell models. (
  • The therapeutic potential of bone marrow-derived mesenchymal stem cells on hepatic cirrhosis. (
  • Culture of cells using various microfluidic devices is becoming more common within experimental cell biology. (
  • They then introduce evolving biological tools for manipulating cell biology more effectively and to reduce costs of bioprocesses. (
  • Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis. (
  • This position will perform a variety of laboratory procedures to support Upstream Process Development, Downstream Process Development, Cell Line Development, Analytical Development, Quality Control, Molecular Biology, or cGMP Manufacturing. (
  • For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (
  • Therefore, a method for detecting microbial growth more quickly would reduce lost batches and be more cost-effective. (
  • Following a review with the US Food and Drug Administration to discuss the potential change to the testing method and validation protocol, Wyeth evaluated rapid microbial testing in its mammalian cell-culture processing facility using the "ScanRDI" ( AES Chemunex , Ker Lann, France) system. (
  • The system uses a three-step process of membrane filtration, direct fluorescent labeling of active cells, and laser scaning for microbial testing. (
  • Chan RCY, Bruce AW, Reid G (1984) Adherence of cervical, vaginal and distal urethral normal microbial flora to human uroepithelial cells and the inhibition of adherence of gram-negative uropathogens by competitive exclusion. (
  • The ideal cell culture medium, whether for use in mammalian or microbial systems, is one that provides process consistency, robustness, and batch-to-batch reproducibility. (
  • In summary, the proposed glucose monitoring and control technique proved to be a reliable tool which can be applied with confidence at an industrial scale for either microbial or mammalian cell cultures. (
  • As a metabolic end product of glucose metabolism, lactate is usually considered to be detrimental to cultures, but after it is synthesized to quite high levels, a number of cell lines will begin to use it through the more efficient citric acid cycle (also known as the tricarboxylic acid cycle or TCA cycle ). (
  • In glucose-limited cells, xylose isomerase was present at low activity but xylulose kinase activity could not be detected. (
  • Among the variables that are appropriate for direct feedback control of the perfusion rate in mammalian cell cultures, high priority should be given to the glucose concentration. (
  • The performance of the perfusion culture was evaluated at four different glucose set points, providing essential information about process optimization. (
  • The on-line glucose concentration was used by an embedded expert system which drove the process through the batch and the perfusion phases, achieving total SCADA control of the feed rate. (
  • The blessed DOWNLOAD ANIMAL CELL CULTURE TECHNIQUES of the MagneGST™ Pull-Down System 's nonfat of Differential Effects, panting Theriomorpha of trainer part, to be Additional parameters of each powerless phosphoacetylation: syndrome glucose. (
  • Develop skills working with: Cell counts using a hemacytometer and spectrophotometer, growth kinetic assays, and glucose oxidase assay. (
  • Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. (
  • It should be noted, when interpreting mitochondrial studies in situ, that mitochondria within cells show heterogeneity in both function and location. (
  • The aim of this course is to introduce the biopharmaceutical industry and its associated processing principles and techniques to Engineers from a non-biopharma background. (
  • Transfection is the process of introducing nucleic acids into eukaryotic cells. (
  • Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. (
  • Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. (
  • Chan R, Acres SD, Costerton JW (1984) Morphological examination of cell surface structures of enterotoxigenic strains of Escherichia coli . (
  • Corning Life Sciences knows cells - you might even say it's in our DNA with over 100 years of experience in the field. (
  • Cell culture bioprocess development is usually carried out at small working volumes. (
  • Capacitance probes have the potential to revolutionize bioprocess control due to their safe and robust use and ability to detect even the smallest capacitors in the form of biological cells. (
  • Errors of transferred models in the declining phase of the culture range for linear models around +100% or worse, causing unnecessary delays with test runs during bioprocess development. (
  • In this pre-recorded webinar, Corning scientists put their expertise in the areas of 3D cell culture and bioprocess to work as they answered some of the commonly asked questions they receive. (
  • If you have a question about 3D cell culture or bioprocess while watching the webinar live, we encourage you to submit it using the questions on the viewing screen and a member of our scientific support team will follow-up with you via email. (
  • this permitted the applicability of this combined technique to bioprocess monitoring to be assessed. (
  • Primary cells are considered by many researchers to be more physiologically similar to in vivo cells. (
  • 3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (
  • Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (
  • This study reports on the effects of fermentor agitation and fed-batch mode of operation on citric acid production from Candida lipolytica using n-paraffin as the carbon source. (
  • It is difficult to solve this problem with the tradeoff between cell yield and production speed by using conventional tactics. (
  • The beauty, if you will, of the cell culture-based vaccine is that it does not have the long lead-in time that we see with egg production," she said. (
  • Bera S, Thillai K, Sriraman K, Jayaraman G (2015) Process strategies for enhancing recombinant streptokinase production in Lactococcus lactis cultures using P170 expression system. (
  • Azotobacter vinelandii OPNA is a potential producer of large quantities of PHB, having ultra-high molecular weight by fed-batch production strategies. (
  • Overall, under optimal feed conditions in the 2.5-l fed-batch culture, cephalosporin C production increased about four times compared to before optimization. (
  • thus, CalB production after 6 weeks was estimated to be about 5.8 times greater than with the fed-batch mode. (
  • This cell line is presently the best source for production of Wnt-3A conditioned medium. (
  • This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. (
  • Building on a long and expert history in cell culture, Corning offers viral vector manufacturing platforms that are easily integrated into modular production set-ups, such as Corning® HYPERStack® and CellSTACK® vessels, which are enabling viral vectors to be produced at large scales. (
  • She furthered developed her career in Belgium, in the field of antibody production in suspension cell lines. (
  • To meet the growing demand for FCDI's iPS cell platform, the state-of-the-art production facility will have a flexible cell culturing design to serve production requirements of both industrial quantities of cells, and small, diverse batches ," said Seimi Satake, Chairman and Chief Executive Officer of FCDI. (
  • Both techniques offer fast production but notably the systems differ in the way they perform complex glycosylation. (
  • Furthermore, cells derived from BTI-TN5B1-4 are used in the production of GlaxoSmithKline's Cervarix [human papillomavirus (HPV) bivalent (types 16 and 18) vaccine, recombinant]. (
  • Technological improvements, such as cell-based vaccines, could make the production cycle even shorter. (
  • In addition, CGTase production is highly dependent on the strain, medium composition and culture conditions. (
  • Cell growth and CGTase production profile were not linked to each other, suggesting that enzyme production/secretion is not growth-associated but mainly a late-log phase event. (
  • The presented protocol enabled analysis of cycling heterogeneities in H. procumbens batch cultivations, and thus should facilitate process control of secondary metabolite production from fragile plant in vitro cultures. (
  • CANNES, France, May 1 -- Two trivalent influenza vaccines, one egg-based and one cell culture-based, displayed similar efficacy during the 2007-2008 flu season, a researcher said here. (
  • In addition, cell culture-based vaccines are not contraindicated for individuals with egg allergy. (
  • Frey S, et al "Demonstrated clinical efficacy of cell-derived and egg-derived influenza vaccines in healthy adult subjects during the 2007-2008 flu season" IVW 2009. (
  • Vaccines could be produced in less than 10 weeks from first isolation of the RNA sequence by using insect cell derived influenza VLPs, according to research which has implications for H1N1 and H5N1. (
  • Extracellular vesicles (EVs) are the cell-secreted nano- and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. (
  • Fluorescence microscopy of the living cells is often achieved through specific labeling of proteins by antibody, nanobody, or bio-specific ligand conjugated to a fluorophore. (
  • However, most of these bio-molecules and fluorophores are not able to cross the cell membrane of a living cell, making it challenging to image intracellular proteins. (
  • Here, we present a general method for labeling intracellular proteins in transfected or non-transfected cells, in the nucleus or the cytoplasm, with probes ranging in size from small (~2 kDa) molecules to large proteins (up to 150 kDa), for either general or super-resolved fluorescence microscopy. (
  • For nucleic acids and vector potency, current analytical techniques for defined CQAs would benefit from further optimization, while for proteins, more complete characterization and mapping of properties to safety and efficacy is needed to finalize CQAs. (
  • Depending on their origin, animal cells grow either as an adherent monolayer or in suspension. (
  • Adherent cells are anchorage-dependent and propagate as a monolayer attached to the cell culture vessel. (
  • Adherent primary cells are particularly susceptible to contact inhibition, that is, they will stop growing when they have reached confluency. (
  • The publication addresses this challenge, describing a special "peptide amphiphile" coating that allows adherent cells to reach a steady balance between growth and detachment. (
  • Strains RC-17 and GR-1 attached in high numbers to uroepithelial cells, whereas T-13 was poorly adherent. (
  • The severity of Plasmodium falciparum infection depends largely on the ability of parasitized red blood cells (PRBC) to adhere on endothelial cells (EC) and sequester in the capillary network of vital organs (e.g. brain, lungs, kidneys, liver. (
  • 4 This offers an immediate advantage in terms of culture metabolism but ultimately makes process definition and development problematic. (
  • Resistance to the proteasome inhibitor bortezomib (BTZ) represents a major obstacle in the treatment of multiple myeloma (MM). The contribution of lipid metabolism in the resistance of MM cells to BTZ is mostly unknown. (
  • Many decades of heuristic optimization have gone into perfecting conventional cell culture devices and protocols. (
  • Optimization of cell culture conditions is necessary to ensure that cells are healthy and in optimal condition for downstream applications. (
  • It was also feasible to increase cell specific productivity through medium and process optimization. (
  • As glycans are so important for biological function, screening glycan profiles early in the clone selection and cell culture optimization process of drug development can provide better information for selecting the right clones. (
  • Glycan acceptance criteria need to be quick and intuitive and allow real-time decisions on clone selection and cell-line optimization. (
  • The kinetics of zinc, cadmium, and lead ions removal by natural zeolite-clinoptilolite has been investigated using an agitated batch adsorber. (
  • These cells express a variety of important receptors driving NK cell recruitment, adhesion as well as activation. (
  • Atorvastatin pre-treatment of EC was found to reduce the expression of adhesion molecules and P. falciparum cytoadherence, to protect cells against PRBC-induced apoptosis and to enhance endothelial monolayer integrity during co-incubation with parasites. (
  • The forced-floating method uses low adhesion polymer-coated culture vessels. (
  • Aliquot suspension to new culture vessels. (
  • Add appropriate aliquots of cell suspension to new culture vessels. (
  • An increase in branched chain amino acids, valine, isoleucine and leucine, and their alpha-keto acid intermediates are known metabolic indicators of mitochondrial dysfunction or respiratory-deficient cells[18,19]. (
  • Selvarasu used available metabolic pathway maps for testing with hybridoma cultures producing monoclonal antibodies (MAbs) in fed-batch mode ( 2 ). (
  • Secondly, using the metabolic activity of the cells to control the feed additions means the cells themselves directly govern the process. (
  • MYC activates different metabolic programs in a cell-type- and cell-status-dependent manner. (
  • Three mechanisms that may contribute to the much higher accumulation of product in the TLFB process are: 1) reduced proteolysis due to lower temperature, 2) reduced proteolysis due to lower cell death and protease release to the medium, 3) increased synthesis rate due to higher AOX activity. (
  • Temperature alone had a significant impact upon the amount of secretory EDIII, with 2.5-fold increase upon reducing the induction temperature from 30 to 20 °C. However surprisingly, supplementation of culture media with Casamino acids (CA), further augmented secretory EDIII titer, with a concomitant drop of intracellular EDIII levels at both temperatures. (
  • Batch experiments at constant temperature have been performed. (
  • During many such biphasic cultures, the initial phase of rapid cell growth at 37 degrees C is followed by a growth arrest phase induced through reduction of the culture temperature. (
  • Differential Phosphoproteomic Analysis of Recombinant Chinese Hamster Ovary Cells Following Temperature Shift. (
  • Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. (
  • Temperature reduction in cultures of hGM-CSF-expressing CHO cells: effect on productivity and product quality. (
  • Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro . (
  • If one batch is contaminated, subsequent batches will be lost. (
  • Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. (
  • During the cell-culture process, manufacturing decisions are made based on retrospective microbiology data. (
  • 0.5 × 10 6 viable cells/mL, so a necessary growth phase of 5-10 days offers little practical value because cell concentrations (and thus initial product yield) during that period will be low. (
  • reported using HD cell banks containing 450 million viable cells/vial to directly inoculate a 20-L Wave bag ( 2 ). (
  • The idea that you can start with a skin cell and make viable eggs in culture is quite amazing. (
  • Online monitoring of viable cell volume (VCV) is essential to the development, monitoring, and control of bioprocesses. (
  • Changes in device material, surface coating, cell number per unit surface area or per unit media volume may all affect the outcome of otherwise standard protocols. (
  • It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. (
  • On the other hand, using a two feeding-pulses fed-batch strategy, the highest global PHB volumetric productivity (0.56 g L −1 h −1 ) and polymer concentration (27.6 g L −1 ) were obtained. (
  • The three-cycle fed-batch mode of operation also increased the final citric acid concentration to 42 g/l from about 12 and 6g/l for single fed-batch cycle and normal batch modes of operation, respectively. (
  • 8 . The method of claim 2 , wherein the human basic fibroblast growth factor is present in the culture in a concentration of at least 0.1 ng/ml for at least a portion of the method. (
  • An understanding and demonstrated competence in aseptic techniques/mammalian cell culture. (
  • The major advantage of using cell culture for any of these applications is the consistency and reproducibility of results that can be obtained from using a batch of clonal cells. (
  • The company's inventoried iCell® products and donor-specific MyCell® Products are available in the quantity, quality, purity and reproducibility required for drug and cell therapy development. (