Encyclopedias as Topic
The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Basophil Degranulation Test
Pituitary Gland, Anterior
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
Fatigue Syndrome, Chronic
A syndrome characterized by persistent or recurrent fatigue, diffuse musculoskeletal pain, sleep disturbances, and subjective cognitive impairment of 6 months duration or longer. Symptoms are not caused by ongoing exertion; are not relieved by rest; and result in a substantial reduction of previous levels of occupational, educational, social, or personal activities. Minor alterations of immune, neuroendocrine, and autonomic function may be associated with this syndrome. There is also considerable overlap between this condition and FIBROMYALGIA. (From Semin Neurol 1998;18(2):237-42; Ann Intern Med 1994 Dec 15;121(12): 953-9)
Xenotropic murine leukemia virus-related virus
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
Blood-sucking acarid parasites of the order Ixodida comprising two families: the softbacked ticks (ARGASIDAE) and hardbacked ticks (IXODIDAE). Ticks are larger than their relatives, the MITES. They penetrate the skin of their host by means of highly specialized, hooked mouth parts and feed on its blood. Ticks attack all groups of terrestrial vertebrates. In humans they are responsible for many TICK-BORNE DISEASES, including the transmission of ROCKY MOUNTAIN SPOTTED FEVER; TULAREMIA; BABESIOSIS; AFRICAN SWINE FEVER; and RELAPSING FEVER. (From Barnes, Invertebrate Zoology, 5th ed, pp543-44)
Evidence for the involvement of IgE-basophil system in acute serum sickness. (1/982)The role of the basophils in acute serum sickness of rabbits was examined by monitoring daily the absolute number of basophils before, during and after the disease period. After antigen (bovine serum albumin, BSA) elimination, levels of serum IgE and in vitro basophil degranulation in the presence of BSA were determined. The results showed that the onset of glomerular lesions depends upon the simultaneous occurrence of circulating immune complexes greater than 19 S and of an in vivo basophil depletion--probably equivalent to degranulation--reaching 70% of the pre-disease number. Post-disease antigen-dependent in vitro degranulation of the basophils and levels of serum IgE anti BSA did not prove to be good indexes of basophil sensitization. Our data suggest that basophils are instrumental at early stages of the deposition of immune complexes, most probably through their sensitization by membrane-bound IgE antibodies. (+info)
Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. (2/982)In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results. (+info)
Extracellular signal-regulated kinases regulate leukotriene C4 generation, but not histamine release or IL-4 production from human basophils. (3/982)Human basophils secrete histamine and leukotriene C4 (LTC4) in response to various stimuli, such as Ag and the bacterial product, FMLP. IgE-mediated stimulation also results in IL-4 secretion. However, the mechanisms of these three classes of secretion are unknown in human basophils. The activation of extracellular signal-regulated kinases (ERKs; ERK-1 and ERK-2) during IgE- and FMLP-mediated stimulation of human basophils was examined. Following FMLP stimulation, histamine release preceded phosphorylation of ERKs, whereas phosphorylation of cytosolic phospholipase A2 (cPLA2), and arachidonic acid (AA) and LTC4 release followed phosphorylation of ERKs. The phosphorylation of ERKs was transient, decreasing to baseline levels after 15 min. PD98059 (MEK inhibitor) inhibited the phosphorylation of ERKs and cPLA2 without inhibition of several other tyrosine phosphorylation events, including phosphorylation of p38 MAPK. PD98059 also inhibited LTC4 generation (IC50 = approximately 2 microM), but not histamine release. Stimulation with anti-IgE Ab resulted in the phosphorylation of ERKs, which was kinetically similar to both histamine and LTC4 release and decreased toward resting levels by 30 min. Similar to FMLP, PD98059 inhibited anti-IgE-mediated LTC4 release (IC50, approximately 2 microM), with only a modest effect on histamine release and IL-4 production at higher concentrations. Taken together, these results suggest that ERKs might selectively regulate the pathway leading to LTC4 generation by phosphorylating cPLA2, but not histamine release or IL-4 production, in human basophils. (+info)
Down-regulation of human basophil IgE and FC epsilon RI alpha surface densities and mediator release by anti-IgE-infusions is reversible in vitro and in vivo. (4/982)Previously, infusions of an anti-IgE mAb (rhumAb-E25) in subjects decreased serum IgE levels, basophil IgE and FcepsilonRIalpha surface density, and polyclonal anti-IgE and Ag-induced basophil histamine release responses. We hypothesized that these effects would be reversed in vivo by discontinuation of infusions and in vitro by exposing basophils to IgE. Subjects received rhumAb-E25 biweekly for 46 wk. Blood samples taken 0-52 wk after rhumAb-E25 were analyzed for serum IgE and basophil expression of IgE, FcepsilonRIalpha, and CD32. Basophil numbers were unaffected by infusions. Eight weeks after infusions, free IgE levels rose in vivo but did not reach baseline. Basophil IgE and FcepsilonRIalpha rose in parallel with free IgE while CD32 was stable. FcepsilonRI densities, measured by acid elution, returned to 80% of baseline, whereas histamine release responses returned to baseline. Basophils cultured with or without IgE or IgG were analyzed for expression of IgE, FcepsilonRIalpha, and CD32. By 7 days with IgE, expression of IgE and FcepsilonRIalpha rose significantly, whereas cultures without IgE declined. IgE culture did not effect CD32. IgG culture did not effect expression of any marker. The present results strongly suggest that free IgE levels regulate FcepsilonRIalpha expression on basophils. (+info)
Adhesive explant culture of allergic nasal mucosa: effect of emedastine difumarate, an anti-allergic drug. (5/982)Allergic reaction of the nose comprises of an immediate and a late reaction. To evaluate nasal allergic reactions, many experiments have been performed by investigators. In this study, we performed a new tissue culture technique (adhesive explant culture) to analyze the migration of cells into the culture medium from the cultured allergic nasal mucosa in response to an allergen. Basophilic cells (mast cells and basophils) and eosinophils, which were released into the culture medium after the allergen challenge, were evaluated by the analysis of histamine and eosinophil cationic protein (ECP) content in the culture medium. Histamine and basophilic cells in the culture medium were more abundant in the immediate phase (within 30 min) after challenge than in the late phase (from 30 min to 10 hr). On the other hand, ECP and eosinophils in the culture medium were more abundant in the late phase than in the immediate phase. The increase of histamine content in both phases were not inhibited by pre-treatment of emedastine difumarate (EME), an anti-allergic drug. However, the increase of ECP in the late phase was inhibited by pre-treatment with EME. Moreover, the number of EG2-positive cells was also decreased by pre-treatment with EME. These results suggest that EME might lower the activation of eosinophils in the late phase of the allergic reaction. The present study also indicates that this adhesive explant culture system is useful model for studying the cellular allergic responses to drugs ex vivo. (+info)
The effect of processing on inflammatory markers in induced sputum. (6/982)The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from healthy and atopic asthmatic subjects were treated with either DTE or phosphate-buffered saline (PBS) at 22 or 37 degrees C and compared for cell counts and concentrations of histamine, tryptase, eosinophil cationic protein (ECP), free interleukin (IL)-8, immunoglobulin (Ig)A, IL-8/IgA complexes and secretory component (SC). In addition, the influence of DTE on in vitro mediator release from blood eosinophils, basophils and bronchoalveolar lavage (BAL) mast cells was studied. Processing with DTE improved cytospin quality and increased the cell yield and measurable ECP, tryptase, IgA and SC, but reduced levels of histamine in PBS-treated samples and had no effect on IL-8. Cell counts or mediator levels were similar when sputum was processed at 22 or 37 degrees C, even though DTE induced blood basophils and BAL mast cells to release histamine at 37 degrees C. In spiking experiments, recovery of added ECP, tryptase, total IL-8 and histamine from sputum was similar in DTE- and PBS-processed sputum, but reduced for free IL-8 in PBS-treated samples. In conclusion, dithioerythritol improves cell and mediator recovery without causing cell activation when sputum processing is conducted at room temperature. The extent of recovery depends on the mediator studied. (+info)
Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway: downregulation by sodium. (7/982)Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 micrograms/ml). However, when extracellular Na+ was substituted iso-osmotically with N-methyl-d-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37 degrees for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM-CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mm, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+. (+info)
Characterization of mast cell-committed progenitors present in human umbilical cord blood. (8/982)Human mast cells are derived from CD34(+) hematopoietic cells present in cord blood, bone marrow, and peripheral blood. However, little is known about the properties of the CD34(+) cells. We demonstrated here that mast cell progenitors that have distinct phenotypes from other hematopoietic cell types are present in cord blood by culturing single, sorted CD34(+) cells in 96-well plates or unsorted cells in methylcellulose. The CD34(+) mast cell-committed progenitors often expressed CD38 and often lacked HLA-DR, whereas CD34(+) erythroid progenitors often expressed both CD38 and HLA-DR and CD34(+) granulocyte-macrophage progenitors often had CD33 and sometimes expressed CD38. We then cultured single cord blood-derived CD34(+)CD38(+) cells under conditions optimal for mast cells and three types of myeloid cells, ie, basophils, eosinophils, and macrophages. Of 1,200 CD34(+)CD38(+) cells, we were able to detect 13 pure mast cell colonies and 52 pure colonies consisting of either one of these three myeloid cell types. We found 17 colonies consisting of two of the three myeloid cell types, whereas only one colony consisted of mast cells and another cell type. These results indicate that human mast cells develop from progenitors that have unique phenotypes and that committed mast cell progenitors develop from multipotent hematopoietic cells through a pathway distinct from myeloid lineages including basophils, which have many similarities to mast cells. (+info)
Regulatory T cells induce activation rather than suppression of human basophils | Science Immunology
Basophils are a rare granulocyte population that has been associated with allergic and inflammatory responses. It is essential to understand the regulatory mechanisms by which basophils are kept in check, considering the impact of dysregulated basophil function on immune responses under different pathological conditions. Among immunoregulatory cells, CD4+CD25+FoxP3+ regulatory T cells (Tregs) are the key players that maintain immune tolerance. The mechanisms by which Tregs regulate and suppress diverse immune cell subsets have been studied extensively, but the impact of Tregs on basophil functions is not well understood. We report that human basophils are refractory to Treg-mediated suppression and found that Tregs stimulate resting basophils to induce the expression of activation markers including CD69, CD203c, and CD13 and the release of basophil cytokines including IL-13, IL-8, and IL-4. Mechanistically, Tregs could induce human basophil activation via IL-3 and STAT5 activation, whereas ...
The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease
RESUMEN. The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes.. Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes in ,0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils.. In addition to clinical history, skin prick test, and specific IgE determination, BAT can be a part of the diagnostic evaluation of patients with food-, insect venom-, and drug allergy and chronic urticaria. It may be helpful in determining the clinically relevant allergen.. Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is less stressful for the ...
Down-regulation of Fc(epsilon)RI expression on human basophils during in vivo treatment of atopic patients with anti-IgE...
Treatment of allergic disease by decreasing circulating IgE with anti-IgE Abs is currently under clinical study. Based on previous unrelated studies, it appeared likely that Fc(epsilon)RI expression on basophils and mast cells might also be regulated by levels of circulating IgE Ab. Therefore, the expression of IgE and Fc(epsilon)RI on human basophils was examined in 15 subjects receiving humanized anti-IgE mAb intravenously. Treatment with the anti-IgE mAb decreased free IgE levels to 1% of pretreatment levels and also resulted in a marked down-regulation of Fc(epsilon)RI on basophils. Median pretreatment densities of Fc(epsilon)RI were approximately 220,000 receptors per basophil and after 3 mo of treatment, the densities had decreased to a median of 8,300 receptors per basophil. Flow cytometric studies, conducted in parallel, showed similar results and also showed in a subset of 3 donors that receptors decreased with a t1/2 of approximately 3 days. The responsiveness of the cells to ...
MHC Class II Expression in Human Basophils: Induction and Lack of Functional Significance
The antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype is a topic of current controversy. We aimed to determine whether human basophils can be induced to express MHC Class II and act as antigen presenting cells for T cell stimulation. Isolated human basophils were exposed to a panel of cytokines and TLR-ligands and assessed for MHC Class II expression. MHC Class II was expressed in up to 17% of isolated basophils following incubation with a combination of IL-3, IFN-γ and GM-CSF for 72 hours. Costimulatory molecules (CD80 and CD86) were expressed at very low levels after stimulation. Gene expression analysis of MHC Class II-positive basophils confirmed up-regulation of HLA-DR, HLA-DM, CD74 and Cathepsin S. However, MHC Class II expressing basophils were incapable of inducing antigen-specific T cell activation or proliferation. This is the first report of significant cytokine-induced MHC Class II up-regulation, at both RNA and protein level, in isolated human ...
Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after...
The basophil activation test (BAT), in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry, is a rapid assay that is gaining popularity. Two markers are currently being evaluated for the BAT; CD63 and the lineage-specific CD203c. In a recent report, detection of CD203c after lysis with Saponin was shown to be superior to detection of CD63 after lysis with formic acid. We wanted to compare a) lysis with formic acid and lysis with Saponin, b) the response through CD203c and CD63, and c) the definition 10% activated cells above background with the probability binning metric T(χ) | 4, on sets of data generated with blood basophils stimulated with varying concentrations of anti-FcεRI antibody. Blood from volunteers was incubated with serial logarithmic dilutions of anti-FcεRI and subsequently with antibodies to CD203c PE and CD63 FITC. Sets of samples set up in parallel were lysed with either Saponin based Whole Blood Lysing reagent or
Cyclosporin A rapidly inhibits mediator release from human basophils presumably by interacting with cyclophilin<...
TY - JOUR. T1 - Cyclosporin A rapidly inhibits mediator release from human basophils presumably by interacting with cyclophilin. AU - Cirillo, Raffaele. AU - Triggiani, Massimo. AU - Siri, Lorenzo. AU - Ciccarelli, Anna. AU - Pettit, George. AU - Condorelli, Mario. AU - Marone, Gianni. PY - 1990. Y1 - 1990. N2 - We have examined the effects of cyclosporin A (CsA) and a series of CsA analogs that bind with decreasing affinity to cyclophilin, to evaluate the involvement of this protein in the release of preformed (histamine) and de novo synthesized (peptide leukotriene C4; LTC4) mediators of inflammatory reactions from human basophils. CsA (8 to 800 nM) concentration-dependently inhibited (5 to 60%) histamine release from peripheral blood basophils challenged with anti-IgE. CsA was more potent (92.6 ± 1.8 vs 59.1 ± 4.5%; p , 0.001) and, at low concentrations, more effective when the channel-operated influx of Ca2+ as bypassed by the ionophore A23187 (IC40 = 24.1 ± 3.9 vs 105.5 ± 22.2 nM; p , ...
Human Basophils Modulate Plasma Cell Differentiation and Maturation | Meta
Basophils represent ,1% of circulating leukocytes. They play a crucial role during allergy and helminth-induced Th2 responses. However, recent data also suggest a contribution to the pathogenesis of autoimmune diseases. Basophils from patients with systemic lupus erythematosus show an activated phenotype, correlating to disease activity. Furthermore, murine basophils or their mediators enhance memory responses and plasma cell (PC) survival, suggesting that they directly modulate the function of B cells. This is highly relevant with respect to human allergy and autoimmunity because a possible modulation of B cell differentiation by basophils could point to new therapeutic targets. Therefore, the interaction between human B cells and basophils and the mechanism underlying this interaction were investigated in detail. Using two different methods to induce PC differentiation, we found that human basophils enhance B cell proliferation, class switching, differentiation into PC, maturation of PC, and ...
Expressions and inhibitory functions of CD300a receptors on purified human basophils - Institutional Repository University of...
The inhibitory myeloid immunoglobulin receptor CD300a (IRp60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to Th2-type immunity and allergy. Basophils homogeneously expressed CD300a as well as the inhibitory receptor CD200R on their cell surface, and these expressions increased after anti-IgE stimulation. IgE-mediated basophil degranulation was also significantly inhibited by crosslinking of either CD200R or CD300a (by 90% and 50%, respectively). Inhibitory SHIP-1 phosphorylations were also induced by CD200R and CD300a, although they were not noticeably increased by IgE-dependent activation. We conclude that both CD200R and CD300a play a role in reducing IgE-mediated basophil function and may crucially govern the known differential ...
Heterogeneity of human basophils and mast cells in response to muscle relaxants<...
TY - JOUR. T1 - Heterogeneity of human basophils and mast cells in response to muscle relaxants. AU - Stellato, C.. AU - de Paulis, A.. AU - de Crescenzo, G.. AU - Tatangelo, F.. AU - Rickler, O.. AU - Marone, G.. PY - 1992/6. Y1 - 1992/6. N2 - We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from ...
Loss of syk kinase during IgE-mediated stimulation of human basophils - Fingerprint - Johns Hopkins University
Fingerprint Dive into the research topics of Loss of syk kinase during IgE-mediated stimulation of human basophils. Together they form a unique fingerprint. ...
Pros and Cons of Clinical Basophil Testing (BAT) - Research Portal, Kings College, London
Purpose of Review: We review basophil testing by flow cytometry with an emphasis on advantages and disadvantages. Recent Findings: There are many tools available to assess the presence and severity of allergic diseases in patients. For 50 years, peripheral blood basophils have been used as tools to study these diseases. It is a very accessible cell that binds IgE antibody and secretes the classical mediators responsible for the symptoms of allergic reactions. In the last decade, an even more accessible methodology, using flow cytometry, has been developed to enhance the ability to use basophils for both mechanistic and clinical diagnostics. Basophil testing has been included in diagnostics for different forms of allergies as well as to monitor disease status. Summary: A variety of studies have begun to establish both precise methods and their clinical relevance for disease diagnosis, but there remain some important questions on how to take optimal advantage of the behaviours of basophils. ...
BioLegend Basophils Category
Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled with histadine, proteolytic enzymes, leukotrienes, and proteoglycans. Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can be identified by expression of CD200R3 and FceRIa. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.
Basophil cell - Wikipedia
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these cell types using standard staining techniques.) *Produced only in pregnancy by the developing embryo. B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology: CD-ROM and Guide. Oxford University Press. ISBN 978-0195151732. MedicalMnemonics.com: ...
Flow cytometric methods for the analysis of human basophil surface antigens and viability<...
TY - JOUR. T1 - Flow cytometric methods for the analysis of human basophil surface antigens and viability. AU - Bochner, Bruce S.. AU - McKelvey, Alicia A.. AU - Schleimer, Robert P.. AU - Hildreth, James. AU - MacGlashan, Donald W.. PY - 1989/12/20. Y1 - 1989/12/20. N2 - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.. AB - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low ...
Effects of the Th2-dominant milieu on allergic responses in der f 1-activated mouse basophils and mast cells<...
TY - JOUR. T1 - Effects of the Th2-dominant milieu on allergic responses in der f 1-activated mouse basophils and mast cells. AU - Yi, Myung Hee. AU - Kim, Hyoung Pyo. AU - Jeong, Kyoung Yong. AU - Kim, Ju Yeong. AU - Lee, In Yong. AU - Yong, Tai Soon. N1 - Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST; grant nos NRF-2012M3A9B8021806 and 2016R1A2B4016194). We are grateful to Min-Ji Song, UnHye Kwon (Yonsei Biomedical Research Institute), and the Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance.. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Although basophils and mast cells share similar phenotypic and functional properties, little is known about the difference in the initial Th2 immune responses of these cells following exposure to proteolytic allergens. Here, we investigated the mechanisms of Th2-mediated immune responses in mouse bone marrow-derived ...
Basophil sensitivity through CD63 or CD203c is a functional measure for specific immunotherapy | Clinical and Molecular Allergy...
Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. Patients (n = 18) starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4, 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c+ cells were plotted against allergen concentration. A sigmoid curve was fitted to the points, and the allergen concentration at which half of the maximal activation was achieved, LC50,
Basophil - Wikipedia
Basophils arise and mature in bone marrow. When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic enzymes (e.g. elastase and lysophospholipase). They also secrete lipid mediators like leukotrienes (LTD-4), and several cytokines. Histamine and proteoglycans are pre-stored in the cells granules while the other secreted substances are newly generated. Each of these substances contributes to inflammation. Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. Interleukin-4 is considered one of the critical cytokines in the development of allergies and the production of IgE antibody by the immune system. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation. The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called ...
Studies on cell-on-chip technology and basophil regulation for improved allergy diagnostics | KTH
Allergic diseases affect approximately 30% of adults and has an impact on both the individuals quality of life as well as an economic impact on society. Two effector cells involved in allergic disease are mast cells and basophils, where basophils are more readily available in blood and therefore of great interest when studying allergy. Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. Allergy diagnostics starts with evaluation of the patients medical history followed by in vivo and/or in vitro testing. All diagnostic tests have different advantages and disadvantages are chosen depending on the patient and the circumstances. In vivo tests include the gold standard of allergy diagnostics, which is the challenge tests, but also the commonly used skin prick test (SPT). Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). BAT is useful to ...
Top Basophil Count Hospitals in Thane | Credihealth
View details of top basophil count hospitals in Thane. Get guidance from medical experts to select best basophil count hospital in Thane
Basophil production in myeloproliferative disorders: increases during acute blastic transformation of chronic myeloid leukemia ...
JA Denburg, WE Wilson, J Bienenstock; Basophil production in myeloproliferative disorders: increases during acute blastic transformation of chronic myeloid leuk
Yvonne Vissers - WUR
Various allergenicity assays have been performed, amongst others T-cell polarisation assays based on cultures of PBMC from allergic and control patients. A cell culture system with rat basophilic leukaemia cells (RBL) has been set up using IgE-containing patients sera to assess the ability of the allergens to respond. Allergens, processed or not have been tested using this model. Furthermore, inhibition studies and basophil histamine release tests are performed in cooperation with partners of the EuroPrevall project.. The immunomodulatory effects of several strains of three Lactobacillus species are assessed. All strains were capable of inducing IL-1β, IL-10, IFN-γ and TNF-α. L. plantarum strains showed a higher induction capacity of IFN-γ and TNF-α compared to the L. acidophilus and L. casei/paracasei strains. A larger variation in effects between species compared to within-species was observed.. ...
Basophil | Cells at Work! Wiki | Fandom
Basophil (好塩基球 Kōenkikyū) is a minor character in Cells at Work!. He is a type of white blood cell, specifically Basophil. He is capable of triggering the release of histamines when encountering an antigen. He also can allure eosinophils and neutrophils when they are needed. His role is not...
Shreffler:SCIT Basophil Activation - OpenWetWare
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study |...
Previous research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). However, uncertainti
Basophils | Article about Basophils by The Free Dictionary
Looking for Basophils? Find out information about Basophils. The following article is from The Great Soviet Encyclopedia . It might be outdated or ideologically biased. , cells whose protoplasm contains granules that... Explanation of Basophils
Desensitization of Human Mast Cells: Mechanisms and Potential Utility for Preventing Anaphylaxis - Full Text View -...
This is an open-label, single-center study of whether oral penicillin desensitization of healthy sensitive subjects results in allergen cross-desensitization of mast cells by skin testing and desensitization of peripheral blood basophils. The primary endpoint(s) will be PC3 prick skin test values to penicillin, aeroallergen(s) and codeine. Secondary endpoints will be levels of Syk in purified basophils determined by flow cytometry (mean fluorescence intensity) and basophil degranulation (% tryptase release) to anti-FcåRI and to calcium ionophore ...
Role of basophils during the initiation/augmentation of Th2 immune responses « Karine SERRE, PhD
A nice review about the early non-T-cell source of IL-4 from basophils that act as Th2-inducing antigen-presenting cells. Even though we have shown that during LN responses to alum-precipitated antigens IL-4 is dispensable for the early induction of Th2-features including B cell maturation and helper T cell differentiation more, it is important to keep in mind that the basophil-source of IL-4 may have implication during the clearance of helminth infections and the development/maintenance/ augmentation of allergic diseases by induction of IgE production, activation of mast cells and basophils, as well as eosinophilic inflammation. An important role for basophils is unquestionable for they have been shown (together with mast cells) to release large amounts of IL-4 and IL-13 after cross-linkage of their IgE receptor, Fce receptor I (FceRI), by complexes of antigen plus antigen-specific IgE. However, recent evidence has proposed a novel role in antigen presentation to CD4 T cells that could play ...
OKAYAMA UNIVERSITY SCIENTIFIC ACHIEVEMENT REPOSITORY
A total of 103 samples of sputum from 20 patients with bronchial asthma were examined for appearance of basophils and eosinophils. The results are as follows: 1) Basophilic leucocytes were found in the sputum of patients with bronchial asthma. 2) Basophils appeared in sputum during asthma attacks and disappeared during nonattack stages. 3) The number of basophils in sputum tended to increase at the end of attack stages. 4) High incidence of basophils in sputum was observed in asthmatic patients with low serum IgE levels. 5) Appearance of basophils in sputum was accompanied with that of eosinophils. 6) Appearance of basophils in sputum was related to the number of peripheral basophils, but not to the daily volume of sputum. These results suggest that basophils migrate from the blood stream into bronchial tissues and appear in sputum during attack stages of bronchial asthma ...
This new book examines the latest research developments in the field of basophil granulocytes, sometimes referred to as basophils, which are the least common of the granulocytes, representing about 0.01% to 0.3% of circulating white blood cells. The name comes from the fact that these leucocytes are basophilic, i.e., they are susceptible to staining by base dyes, as shown in the picture. Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope. However, when unstained, the nucleus is visible and it usually has 2 lobes. The mast cell, a cell in tissues, has many similar characteristics ...
Basophil | blood cell | Britannica.com
Basophil: Basophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by basic dyes and functionally by its role in mediating hypersensitivity reactions of the immune system. Basophils, along with eosinophils and neutrophils, constitute a group of white
Cytochemical method for staining fish basophils: Ingenta Connect
The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. Metachromasia was more identifiable through better preservation of the granules. The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules ...
basophil activation during immune response QuickView - Correlation Engine
A change in morphology and behavior of a basophil resulting from exposure to a cytokine, chemokine, soluble factor, or to (at least in mammals) an antigen which the basophil has specifically bound via IgE bound to Fc-epsilonRI receptors, leading to the in…
Basophil | Lichtmans Atlas of Hematology 2016 | AccessHemOnc | McGraw-Hill Medical
II.E.001. Normal basophil. Blood film. The field contains a basophil and lymphocyte. The dark blue basophilic granules characteristically obscure the nucleus. The granules are relatively large and easily resolved with the light microscope. In this case some cytoplasmic basophilic granules were lost in film preparation and the cell is partially degranulated as a result of the trauma of preparation. This finding is common in blood films.. ...
Type We allergy is characterized by the development of an initial | The Critical Role of Akt in Cardiovascular
Type We allergy is characterized by the development of an initial Th2-dependent allergen-specific IgE response, which is boosted upon subsequent allergen encounter. anti-CD154 only) during sensitization prevented the introduction of allergen-specific IgE, IgM, IgG, and IgA reactions compared to neglected, but sensitized mice. Nevertheless, co-stimulation blockade got no impact on founded IgE reactions in sensitized mice. Immediate type reactions as examined with a rat basophil leukemia cell mediator launch assay were just suppressed by early treatment however, BAY 61-3606 not by co-stimulation blockade after sensitization. CTLA4Ig provided alone didnt suppress both supplementary and major allergen-specific antibody responses. Allergen-specific T cell activation was suppressed in mice by early aswell as past due co-stimulation blockade recommending that IgE-responses in sensitized mice are 3rd party of T cell help. Our outcomes indicate that T cell suppression only without active immune system ...
Hematology: basophil from Vetstream | Definitive Veterinary Intelligence
References of basophilia in ferrets have not been found in the scientific literature, so abnormal basophil counts are believed to be rare or very rare in ferrets ...
Basophil | Definition of Basophil by Merriam-Webster
Define basophil: a basophilic substance or structure; especially : a white blood cell containing basophilic granules that is similar in function to a…
Fondation Recherches 3R / 3R Info Bulletins / Establishment of a novel system for the production of large numbers of mouse...
Fig 3.: Comparable to primary mouse basophils, the Hoxb8 variety is characterized by a relatively short half-life in culture, which was significantly prolonged after the addition of the cytokine interleukin-3 (IL-3). Cell viability (double negative annexin V/PI-population) was assessed by flow cytometry.. In summary, we have established a method to generate basophil-committed progenitor lines using Hoxb8. These progenitors can be induced in vitro to differentiate into mature basophils on a large scale, which, to the best of our knowledge, is a novel and unique attribute for a model of the kind. The here-described cell lines are genetically stable, can in principle be generated from any genetically modified strain of mice that survives up to embryonic stage E12, and can be easily handled in culture. Since large numbers of mice are required to isolate primary basophils, we would like to promote this cellular model as an alternative system to study specific aspects of basophil biology, and ...
Get PDF - Studies on supravital observation of basophils in bronchial asthma in immunological reaction part 1 changes of...
Sato, S., 1975: Studies on supravital observation of basophils in bronchial asthma in immunological reaction part 1 changes of migration velocity of basophils by addition of anti immuno globulin
Basophils are found to be key drivers of allergy-induced lung inflammation
Many particles and molecules in the environment can trigger allergic asthma in susceptible individuals. The allergic response to some of these allergens results in lung inflammation that can lead to a narrowing of the airways and even severe respiratory difficulty. A research team led by Masato Kubo from the RIKEN Center for Integrative Medical Sciences has now identified that a type of immune cell called a basophil is responsible for initiating a cascade of events that leads to inflammation of the lung in mice after exposure to plant- and dust-mite-derived allergens.
Difference between revisions of Shreffler:Basophil tSyk - OpenWetWare
Remove the 2 mL Cryotube with 200 μL of the 20x basophil media, thaw and add 1.8 mL of warm 37°C ) RPMI medium. Close the stopper and vortex it for 5 sec. This is the 2X basophil media to be used for incubation with the whole blood and for preparation of the egg or peanut allergen dilutions (see below ...
Purpose of review Mechanisms involved in the development of type 2 immunity are poorly defined. In addition progenitors that differentiate into mature basophils have recently been recognized. Summary The current review revisits basophils with the goal of providing insights into understanding unappreciated functions of basophils studies of human basophils have provided insights into understanding basophil […]. ...
Neutrophil chemotactic activity in milk-induced asthma.
Four subjects with clinical histories of milk-induced asthma were studied (three allergic to cows milk; one to soya milk). In each instance, skin prick tests, RAST (IgE and IgG4), the basophil histamine release, and serum precipitins, using appropri
The Effect of Xolair on Inhibiting Leukotriene and Cytokine (IL-4 and IL-13) Release From Blood Basophils - Full Text View -...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
Flow cytometry from 7 healthy control, 15 mild-to-moderate and 19 SA patients reveals 31 cells types. Network construction of covariant cells demonstrates neighborhoods that define 4 patient clusters. One SA-enriched cluster shows high levels of IFN-ɣ production driven by T-cells. Other clusters demonstrate varying abundance of innate immune lineages that differ in Type 2 (T2) cytokine elaboration. BEC gene expression analysis identifies 3 clusters, two of which enriched for SA cases. One SA-cluster demonstrates high levels of T2 inflammation and strong relationship with mast cell/basophils in BAL. Another shows low levels of T2 inflammation despite housing a third of SA patients. GSEA identifies interferon and IL-6 pathway activation, suggesting Type 1 (T1) immune response. These data were corroborated with transcriptional profiling of BAL cells, underscoring networks of interacting cytokines between cellular compartments ...
Inflammatory dendritic cells-not basophils-are necessary and sufficient for induction of Th2 immunity to inhaled house dust...
Basophils have gained renewed interest as inducers and amplifiers of Th2 immunity through their potential to migrate to LNs, provide an early source of IL-4, and at the same time act as APCs in models of helminth infection and injection of the model protease papain, but have been largely ignored in the field of pulmonary mucosal immunity and development of allergic asthma to inhaled allergens (Sokol et al., 2008, 2009). Here, we found that basophils are recruited to the MLNs draining the site of i.n. administration of HDM allergen within 3 d of HDM injection. Several mechanisms could explain this increase. Like basophil expansion and recruitment to sites of helminth infection (Voehringer et al., 2006), we found that this process required adaptive immunity and CD4 T cells, as MHCII-deficient mice had severely reduced recruitment of basophils. The most likely explanation is T cell-derived production of IL-3 (Shen et al., 2008; Kim et al., 2010), which we found to be induced in CD4 T cells upon ...
2015年研究業績 | 先端医学研究所 生体調節機構研究部 | 和歌山県立医科大学
H. Sasaki, D. Kurotaki, N. Osato, H. Sato, I. Sasaki, S. Koizumi, H. Wang, C. Kaneda, A. Nishiyama, T. Kaisho, H. Aburatani, H. C. Morse III, K. Ozato, T. Tamura. 2015. Transcription factor IRF8 plays a critical role in the development of murine basophils and mast cells. Blood 125:358-369 ...
Absolute Basophils Count - [Blood] - Book a Test | Afriglobal Medicare
This test is a component of the complete blood count (CBC). CBC is used to screen, diagnose or monitor diseases that affect blood cells eg anemia, infection, inflammation, bleeding problems or cancer. ...
Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases | World Allergy...
Biomarkers of disease activity have come into wide use in the study of mechanisms of human disease and in clinical medicine to both diagnose and predict disease course; as well as to monitor response to therapeutic intervention. Here we review biomarkers of the involvement of mast cells, basophils, and eosinophils in human allergic inflammation. Included are surface markers of cell activation as well as specific products of these inflammatory cells that implicate specific cell types in the inflammatory process and are of possible value in clinical research as well as within decisions made in the practice of allergy-immunology.
Thapsigargin-induced nuclear calcium signals in rat basophilic leukaemia cells | Biochemical Journal
By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells. ...
Basophil degranulation test | Article about basophil degranulation test by The Free Dictionary
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and...
TY - JOUR. T1 - CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections. AU - Bakocevic, Nadja. AU - Claser, Carla. AU - Yoshikawa, Soichiro. AU - Jones, Leigh Ann. AU - Chew, Samantha. AU - Goh, Chi Ching. AU - Malleret, Benoit. AU - Larbi, Anis. AU - Ginhoux, Florent. AU - de Lafaille, Maria Curotto. AU - Karasuyama, Hajime. AU - Renia, Laurent. AU - Ng, Lai Guan. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2014/6. Y1 - 2014/6. N2 - Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45intCD49b+FcεRI+ cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of αIIbβ3 integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis (Nb). Moreover, CD41 can be used as a reliable marker for ...
Application of basophil activation test in diagnosing aspirin hypersensitivity | Gawinowska | Advances in Respiratory Medicine
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic ...
Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells |...
Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat basophilic leukaemia cell line RBL-2H3, and compared their properties with each other and with plasma-membrane vesicles. DRM fractions were isolated as sealed unilamellar vesicles of similar size (135-170 nm diameter), using either sucrose-density-gradient sedimentation or gel-filtration chromatography. Lipid rafts isolated using Brij-96 and Triton X-100 differed in density, protein content and the ...
Basophil substance | definition of basophil substance by Medical dictionary
Looking for online definition of basophil substance in the Medical Dictionary? basophil substance explanation free. What is basophil substance? Meaning of basophil substance medical term. What does basophil substance mean?
Basophilia | definition of basophilia by Medical dictionary
Looking for online definition of basophilia in the Medical Dictionary? basophilia explanation free. What is basophilia? Meaning of basophilia medical term. What does basophilia mean?
Cutaneous basophil responses and immune resistance of guinea pigs to ticks: passive transfer with peritoneal exudate cells or...
Resistance to infestation by larval Amblyomma americanum or Rhipicephalus sanguineus ticks was transferred to naive guinea pigs with peritoneal exudate cells (PEC) or serum from donors immunized by prior infestation with homologous tick larvae. In the A. americanum system, PEC transfer induced 87% tick rejection, which was similar to the level of resistance in actively sensitized hosts. In the R. sanguineus system, PEC conferred resistance (39% rejection) that was weaker than in actively sensitized hosts (57% rejection). In both systems, immune serum conferred significant but weaker resistance (20 to 29% rejection). In actively sensitized hosts, resistance to each tick species was specific, but there was considerable cross-reactive resistance. Basophils dominated the 24-hr challenge feeding sites of A. americanum ticks in actively sensitized hosts (69% of the infiltrate) and recipients of sensitized PEC (69%). Mononuclear cells were dominant (69% of the infiltrate) in the challenged tissues of ...
A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells<...
TY - JOUR. T1 - A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells. AU - Barth, Erin E.. AU - Hallworth, Richard J.. AU - Nichols, Michael G.. PY - 2005/5. Y1 - 2005/5. N2 - Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint will necessarily depend on the characteristics of the damage induced by the agent. By using multiple biological probes, it is possible to get a more complete description of the type of damage induced. Photodamage was induced in rat basophilic leukemia cells by either 254-nm UVC light exposure or rose bengal photosensitization. Damage was measured by three quantitative assays employing fluorescent probes: calcein, to measure nonspecific esterase activity, propidium iodide (PI), to measure loss of plasma membrane integrity, rhodamine 123 ...
Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test<...
TY - JOUR. T1 - Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test. AU - Okamoto, Kaoru. AU - Kagami, Michiko. AU - Kawai, Manabu. AU - Mori, Yuji. AU - Yamawaki, Kazuo. AU - Nakajima, Yoichi. AU - Kondo, Yasuto. AU - Tsuge, Ikuya. PY - 2019/1. Y1 - 2019/1. UR - http://www.scopus.com/inward/record.url?scp=85058961080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85058961080&partnerID=8YFLogxK. U2 - 10.1016/j.alit.2018.08.001. DO - 10.1016/j.alit.2018.08.001. M3 - Letter. C2 - 30190098. AN - SCOPUS:85058961080. VL - 68. SP - 130. EP - 131. JO - Allergology International. JF - Allergology International. SN - 1323-8930. IS - 1. ER - ...
The Basophil activation test: technology of the method and application for clinical practice - Immunopathology, allergology,...
The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus. The Basophil Activation Test (BAT) is a modern cellular method of allergy diagnosis, which due to its high efficiency has firmly taken its place in the diagnostic algorithm of food, pollen and drug allergy, and chronic urticaria. A promising direction of the BAT is to evaluate the effectiveness of specific immunotherapy, treatment with anti-IgE drugs, and the natural resolution of allergy. The current review presents the main approach of the BAT procedure and the efficacy of the use for the diagnosis for various types of allergies, as well as the rules for evaluating the diagnostic results. ...
A Case of Anaphylaxis in Which a Basophil Activation Test Was Used to Identify the Suspected Agent | Anesthesia Progress |...
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
A Case of Anaphylaxis in Which a Basophil Activation Test Was Used to Identify the Suspected Agent | Anesthesia Progress |...
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
Inclusion of clavulanic acid determinants in the basophil activation test improves the evaluation of immediate reactions to...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
Histamine release reaction of basophils in chronic granulocytic leukaemia. Induction by heterologous anti-immunoglobulin E,...
Basophils possess membrane bound IgE molecules, and immunological activation leads to a secretory process with cell degranulation and histamine release. Heterologous anti IgE, concanavaline A, and phytohaemagglutinin are potent non-cytotoxic releasing agents. They operate by a mechanism similar to that of immunological activation. Heavy water is not a histamine releasing inducer but it increases histamine release of the cells. We studied the histamine release reaction of leukaemic basophils in 10 patients and found a physiological response such as that previously reported with normal human basophils.. ...
Mercuric Chloride Enhances IgE-Dependent Mediator Release from Human Basophils. - Kent Academic Repository
Mercuric chloride (HgCl2) is an industrial agent known to cause autoimmune disorders and induce IgE synthesis, which plays a crucial role in the manifestation of allergic diseases. In rodents, the immunomodulatory effects of HgCl2 have been shown to involve the enhancement of mast cell-derived IL-4 secretion, which facilitates both Th2-lymphocyte development and IgE production. In humans, rapid allergen-dependent release of IL-4 and the related cytokine IL-13 from histamine-containing cells occurs primarily in basophils, along with other proinflammatory mediators such as histamine and LTC4. In this study, we therefore investigated the effects of HgCl2 on the release of the above basophil mediators, either due to the compound alone or in conjunction with IgE-dependent stimulation. HgCl2 (10(-9) to 10(-6) M) did not induce mediator secretion alone but significantly enhanced the release of histamine, LTC4, IL-4, and IL-13 caused by anti-IgE. Higher concentrations of HgCl2 (10(-5) to 10(-3) M) ...
The aim of this study was to evaluate whether Na+ exerts its inhibitory effect on basophil histamine release induced by immunoglobulin E (IgE)-dependent (anti-IgE) and IgE-independent (N-formyl-methionyl-leucyl-phenylalanine (FMLP), interleukin-3 (IL-3)) stimuli in patients with allergic rhinitis (n = 24) and allergic bronchial asthma (n = 10). Peripheral blood leucocytes were stimulated with anti-IgE, FMLP and IL-3 in the presence of high and low Na+ concentrations, and histamine release was measured using a fluorometric method. In standard Na(+)-containing medium, spontaneous and stimulated histamine release was higher in allergic patients (n = 34) (both rhinitic and asthmatic) than in healthy subjects (n = 41). Na+ removal from extracellular medium and its isosmotic substitution with choline chloride or with N-methyl-D-glucamine led to a significant increase of anti-IgE-, FMLP- and IL-3-induced histamine release in normal subjects, but not in allergic patients. The increase in Na+ ...
Regulation of human basophil and lung mast cell function by cyclic amp
Peachell P.T.; Macglashan D.W.Jr; Lichtenstein L.M.; Schleimer R.P., 1988: Regulation of human basophil and lung mast cell function by cyclic amp
Publications | Max Planck Institute for Biophysical Chemistry
Parekh, A. B.; Penner, R.: Activation of store-operated calcium influx at resting InsP(3) levels by sensitization of the InsP(3) receptor in rat basophilic leukaemia cells. Journal of Physiology-London 489 (2), pp. 377 - 382 (1995 ...
Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33. - Elabscience
Matsumoto K, Kouzaki H, Kikuoka H, et al. Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33[J]. Allergology International, 2018.
Diamond Basophil Isolation Kit, human - Granulocytes and myeloid cells - MicroBeads and Isolation Kits - Cell separation...
The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs. - Great Britain
D:Basophil Adenoma - wiki-pain
The table below shows the top 200 pain related interactions that have been reported for Basophil Adenoma. They are ordered first by their pain relevance and then by number of times they were reported in Basophil Adenoma. Please click on the INT link to display more detailed information on each interaction. ...
Ovine haemopoiesis: the development of bone marrow-derived colony-forming cells in vitro in the presence of factors derived...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
Hematopoietic growth factor production by cultured cells of human nasal polyp epithelial scrapings: kinetics, cell source, and...
The conditions and cell sources for colony stimulating activity (CSA) production by nasal polyp epithelial scrapings were examined. Epithelial scrapings removed from patients were grown to confluence during 7 days as monolayers of epithelial cells in media supplemented with fetal calf serum (FCS) on collagen-coated microwell plates. Growth kinetics of nasal polyp epithelial cells (NPECs) were determined, and CSA in NPEC conditioned medium (CM) was assessed with density-gradient separated, nonadherent peripheral blood mononuclear cells in standard 14-day methylcellulose assays. Nasal polyp cultures in the presence of 5% or 15% FCS (vol/vol) demonstrated significantly more epithelial cell proliferation than cultures at 0% and 1% FCS. There were comparable metachromatic cell counts in polyp epithelial scrappings from allergic and nonallergic donors. Similarly, NPEC CM from allergic and nonallergic donors had equivalent CSA for basophil/mast cell (BMC) and eosinophil (EO) lineages, respectively. CSA ...
Crystal structures of murine and human histamine-releasing factor (HRF/TCTP) and a model for HRF dimerisation in mast cell...
RePub, Erasmus University Repository: PDE3 Inhibition Reduces Epithelial Mast Cell Numbers in Allergic Airway Inflammation...
Epithelial mast cells are generally present in the airways of patients with allergic asthma that are inadequately controlled. Airway mast cells (MCs) are critically involved in allergic airway inflammation and contribute directly to the main symptoms of allergic patients. Phosphodiesterase 3 (PDE3) tailors signaling of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are critical intracellular second messenger molecules in various signaling pathways. This paper investigates the pathophysiological role and disease-modifying effects of PDE3 in mouse bone marrow-derived MCs (bmMCs), human LAD2- and HMC1 mast cell lines, human blood basophils, and peripheral blood-derived primary human MCs (HuMCs). In a chronic house dust mite (HDM)-driven allergic airway inflammation mouse model, we observed that PDE3 deficiency or PDE3 inhibition (PDE3i) therapy reduced the numbers of epithelial MCs, when compared to control mice. Mouse bone marrow-derived MCs (bmMCs) and the ...
What Is Food Allergy? - ENT Florida
An allergic reaction to food is a two-step process.. Step 1:. The first time you are exposed to a food allergen, your immune system reacts as if the food were harmful and makes specific IgE antibodies to that allergen. The antibodies circulate through your blood and attach to mast cells and basophils. Mast cells are found in all body tissues, especially in areas of your body that are typical sites of allergic reactions. Those sites include your nose, throat, lungs, skin, and gastrointestinal (GI) tract. Basophils are found in your blood and also in tissues that have become inflamed due to an allergic reaction.. Step 2:. The next time you are exposed to the same food allergen, it binds to the IgE antibodies that are attached to the mast cells and basophils. The binding signals the cells to release massive amounts of chemicals such as histamine. Depending on the tissue in which they are released, these chemicals will cause you to have various symptoms of food allergy. The symptoms can range from ...
Ultrastructure of secretory cells in the gut of the cattle-tick Boophilus microplus - UQ eSpace
The ultrastructural study of the secretory cells type 1 and 2 confirmed the separate identities of two secretory cell types in the gut of female B. microplus. Secretory cell type 1 (s1) synthesized and secreted large, spherical, uniformly electrondense granules. Secretory cell type 2 (s2) synthesized smaller, irregularly shaped and more complex granules. Another cell type, the basophilic cell, was shown to be the reorganized basal remnant of secretory cell s2. A few of the basophilic cells retained remnant s2 granules within their cytoplasm. In these cells the reorganized cisternae of rough endoplasmic reticulum were arranged in whorls and parallel arrays. The cells synthesized granules with a different ultrastructure and position in the cell from the earlier granules. The new secretory material may be egg proteins which are released into the haemolymph, and transported to the ovary. Another secretory cell type with smaller spherical granules was seen in the gut caeca of only two female ticks ...
Isolation and Purification of Human Mast Cells and Basophils. - Kent Academic Repository
Gibbs, Bernhard F and Ennis, M. (2001) Isolation and Purification of Human Mast Cells and Basophils. In: Rogers, D. and Donnelly, Louise E., eds. Human Airway Inflammation: Sampling techniques and analytical protocols. The Humana Press Inc., pp. 161-176. ISBN 978-0-89603-923-0. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:4831) ...
FcεRIα Antibody, anti-human, REAfinity™ | Recombinant antibodies | MACS Antibodies | Products | Miltenyi Biotec | Principat d...
Clone REA758 recognizes the human FcεRIα, the α subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one α-, one β-, and two disulphide-linked γ-chains, of which the α subunit binds IgE1. FcεRIα is expressed on mast and basophil cells, is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.Additional information: Clone REA758 displays negligible binding to Fc receptors. | Principat dAndorra
Community Academic Profiles - Faculty & Researchers - Stanford Medicine
Current Research and Scholarly Interests The goals of Dr. Gallis laboratory are to understand the regulation of mast cell and basophil development and function, and to develop and use genetic approaches to elucidate the roles of these cells in health and disease. We study both the roles of mast cells, basophils, and IgE in normal physiology and host defense, e.g., in responses to parasites and in enhancing resistance to venoms, and also their roles in pathology, e.g., anaphylaxis, food allergy, and asthma, both in mice and humans. ...
Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine...
Fingerprint Dive into the research topics of Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine release. Together they form a unique fingerprint. ...
Which of the following cells is found in the cartilaginous tissue of the body? (a) Mast cells (b) Basophils (c) Osteocytes (d)...
Which of the following cells is found in the cartilaginous tissue of the body? (a) Mast cells (b) Basophils (c) Osteocytes (d) Chondrocytes
but not on lymphocytes or basophils. CD15 antigen is important for direct carbohydrate-carbohydrate interaction and plays a...
BCX 1470 methanesulfonate 1). Fig. 4 Behavior from the intestinal Mouse monoclonal to CD15.DW3 reacts with CD15 (3-FAL ), a 220 kDa carbohydrate structure, also called X-hapten. CD15 is expressed on greater than 95% of granulocytes including neutrophils and eosinophils and to a varying degree on monodytes, but not on lymphocytes or basophils. CD15 antigen is important for direct carbohydrate-carbohydrate interaction and plays a role in mediating phagocytosis, bactericidal activity and chemotaxis mucosal microcirculatory factors. a complete vascular thickness. b Perfused vascular thickness. c Percentage of perfused vessels. d Microvascular BCX 1470 methanesulfonate stream index. e Crimson bloodstream cell speed. f Heterogeneity stream index Fig. 5 Behavior from the intestinal serosal microcirculatory factors. a complete vascular denseness. b Perfused vascular denseness. c Percentage of perfused vessels. d Microvascular BCX 1470 methanesulfonate movement index. e Crimson bloodstream cell speed. f ...
Basophil Granulozyt - Wikipedia
Basophile Granulozyten sünd Zellen, de to de Bestanddeelen vunt Blood höört un to de Witten Bloodkörpers (Leukozyten) tellt warrt. De basophilen Granulozyten wiest tallrieke Granula op, de groff un nich regelmatig sünd und ünner annern Histamin un Heparin bargt. Int Differentialbloodbild maakt se blots en lütten Andeel vun 2 bit 6 % ut. De basophilen Granulozyten stammt ut dat Knakenmark, se hebbt mit de eosinophilen Granulozyten en gemeesomen Vörlöper. Wassdomsfakters för de Basophilen sünd ünner annern Interleukin-3, Interleukin-5 un GM-CSF. Dat gifft Henwiesen dorop, dat sik de Basophilen un de Eosinophilen bit Riepen gegensietig kontrolleert. Ton Bispeel ünnerdrückt de transformeeren Wassdomsfakter TGF int Biwesen vun Interleukin-3 dat Differenzeren vun Eosinophilen un föddert dat vun de Basophilen. Se hebbt en Rezepter fört Immunglobulin E (IgE), wat to de Annahmföhrt, dat se en Rull speelt bi de Immunafwehr vunn Weert gegen Parasiten. Wenn jemehr Rezepters dör ...
INKT cells are responsible for the apoptotic reduction of basophils that mediate Th2 immune responses elicited by papain in...
Fingerprint Dive into the research topics of INKT cells are responsible for the apoptotic reduction of basophils that mediate Th2 immune responses elicited by papain in mice following γPGA stimulation. Together they form a unique fingerprint. ...
Regulation of type 2 immunity by basophils - Deakin University Library
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/ typeof=CreativeWork http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/,Regulation of type 2 immunity by basophils, David Voehringer, (electronic resource),/a,,/span, - ,span property=offers typeOf=Offer,,span property=offeredBy typeof=Library ll:Library resource=http://link.library.deakin.edu.au/#Deakin%20University%20Library,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.deakin.edu.au/,Deakin University Library,/a,,/span,,/span,,/span,,/span,,/div ...
What the heck are Basophils? - HealthMatters.io - Lab results explained
A basophil is a type of cell belonging to the white blood cell family. While only comprising a small percentage of the total white blood cells circulating in the blood (normal range: 0.4%-1%), they are the largest type of mature white blood cell. They are created in response to inflammation and infection like all white blood…
Echinacea pallida (EPAL), also known as pale purple coneflower, is a herbaceous flowering plant with immune-enhancement and antioxidative properties. The effect of EPAL on the reproductive performance, serum biochemistry and haematological parameters of rabbit does has been studied here. A total of 100, 21-week-old Grimaud rabbit does, were randomly assigned to two groups. One group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E), while the other was fed the basal diet without the supplementation (control group, C). The reproductive performance of the does was not affected by the treatment (P,0.05). The haematological parameters of pregnant rabbits showed that there was no interaction between gestation day and treatment. The EPAL supplementation induced a reduction (−47.3%) in the basophil cell rate (0.55% and 0.29%, for the control and treatment groups, respectively; P=0.049). The gestation day significantly affected most of the haematological parameters (P,0.05). ...
Download Atlas of Clinical Hematology by L. Heilmeyer, H. Begemann, Helmut Löffler, Johann Rastetter, PDF - Cheng Jiu Jiao Book...
Some monocytes contain azurophilic granules much finer than those seen in lymphocytes. The nucleus is seldom round; usually it is deeply indented and lobulated (often bean-shaped). Its loose, delicate chromatin pattern is unique among the blood cells. Clumps of chromatin may be found scattered among larger, chromatin-poor areas. Nucleoli are rarely present. 55 4 · Individual Cells Fig. 13 a - d a b c d IV 56 Chapter IV · Blood and Bone Marrow Fig. In absolute terms, mitoses are more frequent than in the myeloblasts (Fig. 7 d - h). 39 4 · Individual Cells Fig. 7 a - d a b c d IV 40 Chapter IV · Blood and Bone Marrow Fig. 7 e - h IV e f g h 41 4 · Individual Cells Neutrophilic Myelocytes, Metamyelocytes, Band and Segmented Forms, also Eosinophilic and Basophilic Granulocytes and Tissue Basophils (Fig. 8 a - h) The offspring of the promyelocytes are the myelocytes (Fig. 8 a - e). Generally these cells are somewhat smaller than their immediate precursors, with diameters ranging from 14 to 20 ...
MCP-1 (MCAF, CCL2) (R20-010S): ReliaTech
Rat CCL2 is a member of the β (CC) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
NORMAL STRUCTURE AND DEGENERATIVE CHANGES OF THE CYTOPLASM OF LIVER CELLS AND OF TUMOR CELLS DERIVED FROM THEM | JEM
Discrete bodies which may be designated cytochondria occupy the greater part of the cytoplasm of liver cells. A part, but not all, of these bodies have the characteristics of mitochondria. They consist of a rim which stains deeply and a central part which stains faintly or remains unstained.. In liver cells about portal spaces, in foci of regeneration, and in tumor cells the rims of the cytochondria stain with basic dyes and contain ribonucleic acid or related substances.. With increasing accumulation of basophile substances about cytochondria as the central veins are approached, these bodies aggregate to form clumps or orient themselves with palisade-like arrangment in two rows just within the margins of the liver cell columns.. With aggregation of the cytochondria that are surrounded by basophile material, other cytochondria in the same cells are devoid of this material and wholly acidophile.. With chromatolysis (disappearance of basophile substance) caused by dimethylaminoazobeiizene (butter ...
Ige Antibodies | Technology Trends
is presented to CD4+ Th2 cells specific to the antigen that stimulate B-cell production of IgE antibodies also specific to the antigen ... in type 1 hypersensitivity the antibody is IgE instead of IgA, IgG, or IgM ... During sensitisation, the IgE antibodies bind to Fcε receptors on the surface of tissue mast cells and blood basophils ...
MIB - Histamine
Histamine, b-imidazolethylamine is an intercellular mediator exerting potent effects on various target tissues. Histamine is stored in large amounts in metachromatic granules of mast cells and basophils, and it is one of the major mediators of immediate hypersensitivity. It is released actively from mast cells and basophils when challenged with allergens to which the allergic patient is sensitized or with non-specific histamine releasers such as IgE or pharmacological agents. Histamine is also an important mediator in the central nervous system and the gastric mucosa.. ...
"Basophil". medcell.med.yale.edu. v t e. ...
LTD4 is released by basophils. Other leukotrienes that function in a similar manner are leukotrienes C4 and E4. Pharmacological ...
... basophils, monocytes, and eosinophils; or 2) lymphoid precursor cells which differentiate into T lymphocytes, B lymphocytes, or ...
Basophils have receptors that can bind to IgE, IgG, complement, and histamine. The cytoplasm of basophils contains a varied ... Histamine causes dilation and increased permeability of capillaries close to the basophil. Injured basophils and other ... this polypeptide is produced by basophils and by T-helper 2 cells (TH2). Basophils are one of the least abundant cells in bone ... "Mature Basophil". University of Virginia Health System. Retrieved 2009-04-10. Lee DM, Friend DS, Gurish MF, Benoist C, Mathis D ...
Slow-reacting substance of anaphylaxis
It can be found in basophils. It induces prolonged, slow contraction of smooth muscle and has a major bronchoconstrictor role ...
It is constitutively expressed on mast cells and basophils and is inducible in eosinophils. FcεRI is found on epidermal ... Crosslinking of the FcεRI via IgE-antigen complexes leads to degranulation of mast cells or basophils and release of ... Under laboratory conditions, degranulation of isolated basophils can also be induced with antibodies to the FcεRIα, which ... basophils, and eosinophils". Journal of Allergy and Clinical Immunology. 117 (2 Suppl Mini-Primer): S450-5456. doi:10.1016/j. ...
Fragment crystallizable region
... basophils, and eosinophils; and other processes). In a new development in the field of antibody-based therapeutics, the Fc ...
White blood cell differential
Basophil Basophils exhibit large, dark purple granules that often cover the cell's nucleus. They are the rarest of the five ... Basophils are often quantified using a reagent that lyses the cytoplasm of other white blood cells but leaves basophils intact ... An increased basophil count may also be seen in hypersensitivity reactions and after splenectomy. The basophil count may ... ISBN 978-0-8036-6825-6. Gibbs, Bernhard (2014). "The absolute basophil count". Basophils and mast cells : methods and protocols ...
Basophils leave the bone marrow already mature, whereas the mast cell circulates in an immature form, only maturing once in a ... The mast cell is very similar in both appearance and function to the basophil, another type of white blood cell. Although mast ... The Fc region of immunoglobulin E (IgE) becomes bound to mast cells and basophils and when IgE's paratopes bind to an antigen, ... Mast cells are very similar to basophil granulocytes (a class of white blood cells) in blood. Both are granulated cells that ...
Prussin C, Metcalfe DD (February 2006). "5. IgE, mast cells, basophils, and eosinophils". The Journal of Allergy and Clinical ... mast cells and basophils. As a result of its cellular distribution, this receptor plays a major role in controlling allergic ... and basophils. Fc receptors are found on a number of cells in the immune system including phagocytes like macrophages and ... basophils, human platelets, and mast cells - that contribute to the protective functions of the immune system. Its name is ...
Mast cell leukemia
Characteristically, basophil (e.g. CD11b, CD123) and monocyte markers (CD14, CD15) are absent. The cells usually express CD2 ...
Evidence and efficacy of homeopathy
The paper purported to have discovered that basophils, a type of white blood cell, released histamine when exposed to a ... Hirst, SJ; Hayes, NA; Burridge, J; Pearce, FL; Foreman, JC (1993). "Human basophil degranulation is not triggered by very ... "Histamine dilutions modulate basophil activation". Inflammation Research. 53 (5): 181-88. doi:10.1007/s00011-003-1242-0. PMID ... "Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties". Experientia. 48 (5): ...
The granulocytes are neutrophils, eosinophils, basophils, and mast cells. Mononuclear cell infiltrates are characteristic of ...
Innate immune system
Basophils and eosinophils are cells related to the neutrophil (see above). When activated by a pathogen encounter, histamine- ... Neutrophils, along with two other cell types (eosinophils and basophils; see below), are known as granulocytes due to the ... The innate leukocytes include: natural killer cells, mast cells, eosinophils, basophils; and the phagocytic cells include ... releasing basophils are important in the defense against parasites and play a role in allergic reactions, such as asthma. Upon ...
It also stimulates basophils to release B cell homeostatic factors. This is consistent with the reduction in the number of ... IgD may have some role in allergic reactions. Recently, IgD was found to bind to basophils and mast cells and ... and basophil, respectively. Counter-intuitive to the conventional paradigm that activation of the immune system may potentiate ... "Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44". Immunity. 49 (4): 709- ...
... B is also found on basophils, neutrophils, monocytes, and macrophages. CD32B can be found on airway smooth muscle cells, as ... CD32A is also found on Langerhans cells, mast cells, basophils, eosinophils, monocytes, megakaryocytes, and a subpopulation of ...
The role of IgE, basophils, and a platelet-activating factor". The Journal of Experimental Medicine. 136 (6): 1356-77. doi: ... PAF is produced by stimulated basophils, monocytes, polymorphonuclear neutrophils, platelets, and endothelial cells primarily ... a new mediator of anaphylaxis and immune complex deposition from rabbit and human basophils". Nature. 249 (457): 581-2. Bibcode ...
Type I hypersensitivity
Mast cells and basophils coated by IgE antibodies are "sensitized". Later exposure to the same allergen cross-links the bound ... During sensitization, the IgE antibodies bind to FcεRI receptors on the surface of tissue mast cells and blood basophils. ...
Reprinted in Cushing H (April 1969). "The basophil adenomas of the pituitary body". Ann R Coll Surg Engl. 44 (4): 180-1. PMC ... However, the most likely explanation, proposed by J. Aidan Carney and based on statistical evidence, was that the basophil ... ISBN 978-0-470-67201-3. Cushing, Harvey (1932). "The basophil adenomas of the pituitary body and their clinical manifestations ... then an excess of basophil cells must be involved in another pituitary disorder that involves sexual dysfunction (amenorrhea in ...
1932 in science
Reprinted in Cushing, Harvey (April 1969). "The basophil adenomas of the pituitary body". Annals of the Royal College of ... ISBN 978-0-89093-547-7. CS1 maint: discouraged parameter (link) Cushing, Harvey (1932). "The basophil adenomas of the pituitary ...
Basophil activation Yamasaki S, Saito T (2005). "Regulation of mast cell activation through FcepsilonRI". Chem Immunol Allergy ... It is used by several different cells involved in the immune system, including granulocytes (neutrophils, basophils, and ...
White blood cell
Main article: Basophil. Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine ... Basophil. 0.4%. 12-15. *Release histamine for inflammatory responses. Bi-lobed or tri-lobed. Large blue. A few hours to a few ... Basophils can also release chemical signals that attract eosinophils and neutrophils to an infection site. ... basophils, lymphocytes, and monocytes. These types are distinguished by their physical and functional characteristics. ...
Basophils have receptors that can bind to IgE, IgG, complement, and histamine. The cytoplasm of basophils contains a varied ... Histamine causes dilation and increased permeability of capillaries close to the basophil. Injured basophils and other ... Basophils are one of the least abundant cells in bone marrow and blood (occurring at less than two percent of all cells). Like ... When an infection occurs, mature basophils will be released from the bone marrow and travel to the site of infection. When ...
Peripheral blood mononuclear cell
Basophils are sometimes found in both the denser and the PBMC fractions. Recent studies indicate that PBMCs may be susceptible ... basophils, and eosinophils) have multi-lobed nuclei. In humans, lymphocytes make up the majority of the PBMC population, ...
65, 2014, S. 559-566, doi:10.1007/s00105-014-2815-2. Mast cell Basophil Food intolerance Red wine headache "HIT > Histaminosis ... mast cells and basophils), versus the breakdown of histamine by the enzymes which metabolize it, such as diamine oxidase (DAO) ... from mast cells and basophils throughout the body. Possible symptoms after ingestion of histamine-rich food include: Skin rash ...
Acute basophilic leukemia
May manifest basophil and mast cell granules by EM. Cytogenetically heterogeneous but frequently associated with Philadelphia ... Acute basophilic leukemia is a rare form of acute myeloid leukemia where blasts are accompanied by abnormal basophils in all ... It would most likely be classified as M0 without electron microscopic confirmation of basophil lineage. Differentiated ( ...
It contains colloid-filled cysts and two types of cells - basophils and chromophobes. The cysts are the remainder of Rathke's ...
Valent, Peter; Bettelheim, P. (1992). "Cell surface structures on human basophils and mast cells: biochemical and functional ... "Cell surface structures on human basophils and mast cells: biochemical and functional characterization. Adv Immunol." (52): 333 ...
Hematopoietic stem cell
Myeloid cells include monocytes, macrophages, neutrophils, basophils, eosinophils, erythrocytes, and megakaryocytes to ... Colony-forming unit-basophil (CFU-B) Colony-forming unit-eosinophil (CFU-Eos) The above CFUs are based on the lineage. Another ...
This reaction takes place in Golgi apparatus in mast cells and in basophils. Next, histamine is stored in granularity in mast ... cells as a complex with acid residues of heparin proteoglycan while in basophils as a complex with chondroitine sulfate. ...
Basophil - Wikipedia
This suggests that basophils may play a role in the immune response to these viruses. The role of basophils in the immune ... Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in ... Basophils are a type of white blood cell. Basophils are the least common type of granulocyte, representing about 0.5% to 1% of ... The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test ...
Basophil cell - Wikipedia
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil ... These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these ... B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology ...
Basophil - Wikipedia
"Basophil". medcell.med.yale.edu.. *^ Voehringer D (December 2009). "The role of basophils in helminth infection". Trends in ... The word basophil uses combining forms of baso- + -phil, yielding "base-loving". ... Basophils are a type of white blood cell. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ... The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test ...
Basophils and Mast Cells | SpringerLink
In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these ... In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these ... Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future ... Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease ...
Basophils: Definition, function, and normal range
Basophil tests can help doctors diagnose a range of health problems, including autoimmune conditions and allergies. Learn more ... Basophils are a type of white blood cell that plays a vital role in keeping the immune system functioning. ... about the function of basophils and what high and low levels mean in this article. ... If basophil levels are low, this may be a sign of an allergic reaction or another condition. High basophil levels may indicate ...
Basophils: Definition, function, and normal range
Basophil tests can help doctors diagnose a range of health problems, including autoimmune conditions and allergies. Learn more ... Basophils are a type of white blood cell that plays a vital role in keeping the immune system functioning. ... about the function of basophils and what high and low levels mean in this article. ... What are basophils?. Share on Pinterest. Basophils are a type of white blood cell, which are vital components of the immune ...
Basophil | Encyclopedia.com
basophil* A type of white blood cell (leucocyte ) that has a lobed nucleus surrounded by granular cytoplasm (see granulocyte ... 2]). Basophils are produced continually by stem cells in the red bone marrow  and move about in an amoeboid fashion. ... 2. an increase in the number of basophils in the blood.. Cite this article Pick a style below, and copy the text for your ... basophil (bay-sŏ-fil) 1. n. a variety of white blood cell (see polymorph) distinguished by the presence in its cytoplasm of ...
basophil degranulation | PNAS
Cutaneous Basophil Hypersensitivity | SpringerLink
The term cutaneous basophil hypersensitivity (CBH) initially referred to a systemic but evanescent pattern of delayed-onset ... Dvorak, H. F., Colvin, R. B., and Churchill, W. H., 1975, Specificity of basophils and lymphocytes in cutaneous basophil ... Dissociation between cutaneous basophil hypersensitivity and basophil sensitization. J. Immunol. 129: 1262-1266.PubMedGoogle ... Rejection of ticks from guinea pigs by anti-hapten-antibody mediated degranulation of basophils at cutaneous basophil ...
Basophil | blood cell | Britannica.com
Basophils, along with eosinophils and neutrophils, constitute a group of white ... Basophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by basic dyes ... blood: Basophils. Basophils are the least numerous of the granulocytes, and their large granules almost completely obscure the ... More About Basophil. 6 references found in Britannica articles. role in. immunity. * In immune system: Granulocytes ...
Cytochemical method for staining fish basophils: Ingenta Connect
Basophil (close-up): MedlinePlus Medical Encyclopedia Image
Basophils are a specific type of white blood cell. These cells are readily stained with basic dyes (this is where the name ... of this basophil. Basophils make up only a small portion of the number of white blood cells but are important parts of the ... Basophils are a specific type of white blood cell. These cells are readily stained with basic dyes (this is where the name ...
Smoking and blood basophils. | Thorax
Low molecular weight thymic factor inhibits histamine release from basophils
How does the Immune System Use Basophils? (with pictures)
The immune system uses basophils to release histamine, which increases blood flow to the part of the body thats under attack ... A diagram of white blood cells, including basophils. Basophils are part of the bodys innate immunity. In other words, these ... The primary purpose of basophils is to release histamine in response to an antigen, or an allergen such as pet dander. The ... Basophils are part of the bodys innate immune system that will be present at birth. ...
BioLegend Basophils Category
Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils ... Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled ... can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can ...
I Always fall asleep, dizzy, high basophils in blood - Chronic Fatigue Syndrome - MedHelp
UCB Basophil Chip-IHC from 3H Biomedical AB
Umbilical cord blood basophils, For immunohistochemistry (IHC),biological,biology supply,biology supplies,biology product ... UCB Basophil Chip-IHC from 3H Biomedical AB, ... UCB Basophil Chip-IHC from 3H Biomedical AB. Company. 3H ... Basophil Chip-IHC from 3H Biomedical AB. 2. UCB Neutrophil Chip-IHC from 3H Biomedical AB. 3. MoDC Cell Chip-IHC from 3H ... Umbilical cord blood basophils, For immunohistochemistry (IHC). Info. 3H Biomedical AB. Dag Hammarskjlds vg 34, House Generalen ...
BioLegend Basophils Antibodies Products
Clinical Significance Of Blood Formation In Basophils, Monocytes, Thrombocytes And Lymphocytes | HubPages
BASOPHILS. Basophils form up to 1% of the total leucocytes. They are identified by the presence of coarse basophilic granules ... Increase in basophil leucocytes (counts above 0.1 X 10^9/liters) is uncommon, but this is seen in myeloproliferative disorders ... Disorders like myxedema, smallpox, chicken pox and ulcerative colitis also may be associated with basophil leucocytosis. ... Clinical Significance Of Blood Formation In Basophils, Monocytes, Thrombocytes And Lymphocytes. Updated on January 18, 2014 ...
Human Basophils Modulate Plasma Cell Differentiation and Maturation | The Journal of Immunology
Effects of basophils and basophil supernatants on B cell CD23 and CD40 expression. Basophils are known to produce mediators ... Therefore, the impact of basophils, basophil-supernatants, and basophil-mediators on the upregulation of CD23 and CD40 on B ... and basophil mediators on B cell CD23 and CD40 expression was investigated. Our data demonstrate that basophils and basophil- ... Basophils enhance the numbers of CD23+ and CD40+ B cells. Enhancement of (A) CD23+ or (B) CD40+ B cell numbers by basophils ...
Basophil (close-up) | Health Encyclopedia | FloridaHealthFinder.gov
A month later I had a repeat, platelets back to normal but slightly raised basophil. She said I might of had an infection or ... Raised basophil Hi, bit of a long one. Last year I had really bad health anxiety. I was convinced My glands in My neck were ... Re: Raised basophil Thank you for your reply. I did the worse thing and googled what it could mean when I got the results last ... Re: Raised basophil Those are inflammation markers. You had some swollen glands and then a cold sore... Cause = Effect. Told ...
Basophil activation test for the in vitro diagnosis of nonsteroid...: Ingenta Connect
A Basophil Activation Index (percentage of activated basophils after allergen stimulation/percentage of basally activated ... The basophil activation test is useful for the in vitro diagnosis of NSAID hypersensitivity, providing good specificity and ... Keywords: Anaphylaxis; CD63; NSAID; asthma; basophil activation; diagnostic test; drug hypersensitivity; in vitro; intolerance ... Basophil activation test for the in vitro diagnosis of nonsteroidal anti-inflammatory drug hypersensitivity ...
JCI - Selective ablation of basophils in mice reveals their nonredundant role in acquired immunity against ticks
Diphtheria toxin administration to these mice selectively ablated basophils. Diphtheria toxin-mediated basophil depletion ... as was IgFc receptor expression on basophils but not on mast cells. The infiltration of basophils at tick-feeding sites ... mMCP-8-expressing basophils were stained in brown. Scale bars: 100 μm. Data shown in A. -. C. are representative of at least 3 ... Changes in the number of basophils during the infestations are shown. Data are shown as mean ± SEM, n. = 3 each. (. C. ) Tick- ...
Effect of Omalizumab (Xolair) on Basophils in Patients With Chronic Idiopathic Urticaria - Tabular View - ClinicalTrials.gov
Effect of Omalizumab (Xolair) on Basophils in Patients With Chronic Idiopathic Urticaria. The safety and scientific validity of ... Change in the basophil proteome [ Time Frame: Baseline through week 13 ]. In patients with chronic urticaria who respond ... Change in basophil proteome in responders to omalizumab compared to non-responders to omalizumab [ Time Frame: Baseline through ... The purpose of this study is to evaluate for changes in the proteins produced in white blood cells (basophils) in patients with ...
Basophil granulocyte - wikidoc
Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in ... For the cells of the anterior pituitary see: anterior pituitary basophil.. Basophils are the least common of the granulocytes, ... When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic ... Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T ...
Basophil g's | definition of basophil g's by Medical dictionary
... basophil gs explanation free. What is basophil gs? Meaning of basophil gs medical term. What does basophil gs mean? ... Looking for online definition of basophil gs in the Medical Dictionary? ... redirected from basophil gs). Also found in: Dictionary, Thesaurus, Encyclopedia.. Related to basophil gs: eosinophilic, Mast ... Basophil gs , definition of basophil gs by Medical dictionary https://medical-dictionary.thefreedictionary.com/basophil+g%27s ...
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GranulocytesRole of basophilsGranulesAllergenGranulocyteBasophilicInflammationVitroFlow cytometryAbsolute Basophil CountCD203cHistamine release from basophilsBone marrowAllergyEffector cellsDifferentiationExpression on basophilsMiceCellsRelease histamineNeutrophilsMurine BasophilsActivate basophilsAntibodyImmune systemAllergic patientsAdenomaDepletionCytokineChronicBloodCytoplasmReceptorFMLPMediatorsEosinophilAbnormal basophilGranularStimulationHeparinLeukocyteResponsivenessHumanFact that basophilsOccurrence of basophilsActivation of BasophilsImmunologyAnterior pituitaryMonocytesDiagnosisReceptorsLymphocytesPeripheral basophilsAllergensSpleenInflammatory
- Basophils are the least common of the granulocytes , representing about 0.5 to 1% of circulating white blood cells . (wikipedia.org)
- Like all circulating granulocytes, basophils can be recruited out of the blood into a tissue when needed. (wikipedia.org)
- Basophils, along with eosinophils and neutrophils , constitute a group of white blood cells known as granulocytes . (britannica.com)
- Basophils are the least numerous of the granulocytes and account for less than 1 percent of all white blood cells occurring in the human body . (britannica.com)
- Basophil granulocytes (basophils) represent a small leukocyte population that is known for its role in allergy and helminth infections. (jimmunol.org)
- The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes. (cun.es)
- a transient increase in free allergen specific immunoglobulin IgX (mainly, but not exclusively IgG4 and IgA) and a decrease in the ratio of free allergen specific immunoglobulin to allergen specific IgE bound to effector cells (mast cells and basophil granulocytes) [ 4 , 5 ]. (pubmedcentralcanada.ca)
- Basophils are granulocytes that exist at a relatively rare frequency in the blood but are critical mediators of allergic and inflammatory responses. (sciencemag.org)
- The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs. (miltenyibiotec.com)
- Basophilic granulocytes, also known as basophils, are a rare population of leukocytes with frequencies below 1% in peripheral blood. (miltenyibiotec.com)
- Basophilic granulocytes (basophils) are a rare leukocyte population within the blood, playing crucial roles in innate immune defense, immune regulation and allergic responses. (forschung3r.ch)
- Basophils are rare granulocytes that are most commonly found in peripheral blood and tissues. (stemexpress.com)
Role of basophils6
- Doctors believe that the role of basophils in the body is reactionary, meaning that their number will generally only rise or fall due to an invader or underlying chronic issue. (medicalnewstoday.com)
- The role of basophils in the immune response to these viruses is further supported by findings that the CD200 receptor is expressed more frequently in basophils than in other circulating leukocytes. (wikipedia.org)
- The results provide the basis for further investigations on the modulating role of basophils in diseases such as allergy and autoimmunity. (jimmunol.org)
- Aims The essential role of basophils as an initiator of chronic allergic reaction has been elucidated in mouse models. (bmj.com)
- The existence of basophils at the centre of inflammatory cells suggests the role of basophils as an initiator of chronic allergic conjunctivitis. (bmj.com)
- Research Roundup: Using the flu virus to eliminate prostate cancer, the role of basophils in cancer and more! (speakingofresearch.com)
- Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope when stained. (wikipedia.org)
- The granules inside basophils contain heparin, histamine, and other molecules that play a role in inflammation . (medicalnewstoday.com)
- The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules. (ingentaconnect.com)
- 2. basophilic granules in the basophils of the adenohypophysis. (thefreedictionary.com)
- Basophils are easily recognized because of their large, dark granules. (labce.com)
- The color of the basophil cytoplasm is light pink to light blue or colorless, but the specific basophilic granules are prominent making the cytoplasm difficult to see. (labce.com)
- These cells had lobulated nuclei and granules characteristic of basophils. (rupress.org)
- When examining a blood film you may find that some basophils have many dense granules, as seen in the upper image on the right, while others appear washed out with only a few granules, as shown in the lower image. (labce.com)
- When responding to an allergen, basophils that sustain damage will release histamine, which is partially responsible for inflammation during an allergic reaction. (medicalnewstoday.com)
- The primary purpose of basophils is to release histamine in response to an antigen, or an allergen such as pet dander. (wisegeek.com)
- Nearby basophils, confronted with the allergen, release histamine. (wisegeek.com)
- A Basophil Activation Index (percentage of activated basophils after allergen stimulation/percentage of basally activated basophils) of two or more was considered a positive result. (ingentaconnect.com)
- Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). (kth.se)
- In paper II, we further investigated whether it was possible to detect a dose-dependent allergen activation for basophils captured in a microfluidic chip. (kth.se)
- We detected a significant difference in CD63-expression between the negative control and allergen-activated basophils from allergic patients but no difference between the negative control and the non-relevant allergen (an allergen to which the patient had no IgE antibodies). (kth.se)
- To evaluate the hypothesis that basophil anergy occurs in vivo due to chronic allergen exposure in the setting of a clinical oral immunotherapy trial. (nih.gov)
- Basophil reactivity to in vitro stimulation with peanut allergen and controls was assessed by the upregulation of activation markers, CD63 and CD203c, measured by flow cytometry. (nih.gov)
- The pathological roles of eosinophils, mast cells, and basophils in allergy are either directly or indirectly linked with the presence of allergen-specific IgE in allergic individuals. (worldallergy.org)
- Basophils will be enriched and stimulated with allergen and controls. (clinicaltrials.gov)
- This is the 2X basophil media to be used for incubation with the whole blood and for preparation of the egg or peanut allergen dilutions (see below). (openwetware.org)
- In mice whose basophils were unable to produce IL-4, protease treatment did not cause lung inflammation, suggesting that basophil-derived IL-4 could be the main driver of protease-allergen-induced asthma in mice-a finding that could also extend to humans. (medicalxpress.com)
- Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. (cun.es)
- Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. (pubmedcentralcanada.ca)
- Basophils were identified as CD203c + leukocytes, and the proportion of CD63 + and CD203c + cells were plotted against allergen concentration. (pubmedcentralcanada.ca)
- Basophil activation is a versatile and sensitive tool that measures changes in the humoral immune response to allergen during SCIT. (pubmedcentralcanada.ca)
- Although it may also have diagnostic potential, its real power emerges when it is used to determine basophil sensitivity with serial dilutions of allergen, where it correlates well with clinical symptom strength [ 5 , 10 ]. (pubmedcentralcanada.ca)
- The flow assay stimulation test (FAST) uses flow cytometry to determine the percentage of basophils that express CD63 as an activation marker after in vitro stimulation with allergen. (cun.es)
- The ratio of the percentage of CD63+ basophils after stimulation with peanut and after stimulation with anti-IgE (CD63 peanut/anti-IgE) was independently associated with severity (P= .001), whereas the basophil allergen threshold sensitivity CD-sens (1/EC50 × 100, where EC50 is half maximal effective concentration) value was independently associated with the threshold (P= .020) of allergic reactions to peanut during OFCs. (ovid.com)
- Non-specific (anti-IgE), helminth-specific and HDM-allergen-specific basophil histamine release (HR), plus helminth- and HDM-specific IgE and IgG4 responses were measured pre- and post-treatment. (gwu.edu)
- Signaling pathways activated by a protease allergen in basophils. (ox.ac.uk)
- A cysteine protease, papain, is a prototypic protease allergen that can directly activate basophils and mast cells, leading to the production of cytokines, including IL-4, characteristic of the type 2 immune response. (ox.ac.uk)
- Collectively, these data characterize the downstream signaling pathway activated by a protease allergen in basophils. (ox.ac.uk)
- basophil A type of white blood cell ( leucocyte ) that has a lobed nucleus surrounded by granular cytoplasm (see granulocyte ). (encyclopedia.com)
- A basophil is also known as a basophilic granulocyte or a baso. (labce.com)
- Basophils are a rare granulocyte population that has been associated with allergic and inflammatory responses. (sciencemag.org)
- Despite being first described in humans nearly 130 years ago, the basophil granulocyte has received little recognition other than being the least common leukocyte circulating in blood. (elsevier.com)
- There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation. (wikipedia.org)
- High levels of basophils may indicate chronic inflammation in the body. (medicalnewstoday.com)
- Eosinophils also release chemicals that contribute to the inflammation started by basophils, thus further attracting more white blood cells to an affected area. (wisegeek.com)
- Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. (kth.se)
- Activated basophils release vasoactive granular mediators and secrete cytokines, especially of the Th2 type, that promote allergic inflammation. (jimmunol.org)
- Supporting their contribution in allergy, basophils were found at sites of allergic inflammation, where they displayed an activated phenotype in asthma ( 2 ) or atopic dermatitis ( 3 ) patients. (jimmunol.org)
- Thus, IgE-induced chronic inflammation was abrogated in the absence of basophils ( 4 , 5 ). (jimmunol.org)
- Figure 1: Lung inflammation that causes asthma is linked to the secretion of interleukin-4 (IL-4) from immune cells called basophils. (medicalxpress.com)
- A research team led by Masato Kubo from the RIKEN Center for Integrative Medical Sciences has now identified that a type of immune cell called a basophil is responsible for initiating a cascade of events that leads to inflammation of the lung in mice after exposure to plant- and dust-mite-derived allergens. (medicalxpress.com)
- While investigating the triggers of this inflammation, the researchers found that these protease allergens induced lung inflammation in normal mice with a complete immune system, but not in mice genetically engineered to lack basophils. (medicalxpress.com)
- The extent to which basophil-derived IL-4 is involved in the induction of lung inflammation, however, was unknown. (medicalxpress.com)
- The findings suggest that treatments that reduce the numbers of basophils, or that prevent the production of IL-4 by basophils, could be promising for the management of lung inflammation and asthma caused by protease allergens," says Kubo. (medicalxpress.com)
- Allergic inflammation is characterized by selective accumulation of Th2 cells, eosinophils, and basophils, and is often triggered by antigen-stimulated mast cells ( 3 )( 4 ), although the mechanisms supporting these phenomena are largely unclear. (rupress.org)
- By showing dysregulation of IgD class-switched B cells and 'IgD-armed' basophils in autoinflammatory syndromes with periodic fever, our data indicate that IgD orchestrates an ancestral surveillance system at the interface between immunity and inflammation. (lu.se)
- The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test (BAT). (wikipedia.org)
- IL-3 amplified the number of PC in vitro, and acted synergistically with basophils in enhancing Ab production. (jimmunol.org)
- Basophil activation test for the in vitro diagnosis of nonsteroid. (ingentaconnect.com)
- The basophil activation test is useful for the in vitro diagnosis of NSAID hypersensitivity, providing good specificity and positive predictive value and diagnostic reliability in the assessment of NSAID intolerance. (ingentaconnect.com)
- The aims of this thesis were to improve the existing BAT using microfluidic techniques to enable fast and cheap point-ofcare (POC) diagnostics as well as to further study basophil adhesion and activation for a better understanding of basophil behavior and regulation both in vivo and in vitro in a microfluidic chip. (kth.se)
- Compared to the control group, patients with NCWSS and multiple food sensitivity revealed significantly higher incidence of food allergy in the paediatric age group, and in the groups with coexistent atopic diseases, positive serum IgG anti-gliadin antibodies (AGAs) and IgG anti-betalactoglobulin antibodies, in vitro basophil activation, and eosinophil presence in the colon mucosa biopsies. (thefreedictionary.com)
- Peanut OIT induces a hyporesponsive state in basophils that is consistent with pathway-specific anergy previously described in vitro. (nih.gov)
- The distribution of percent CD63 high basophils after in vitro stimulation from subjects over time by treatment status during the blinded portion of the OIT study. (nih.gov)
- Thereafter, the effect of basophils on autoantibody production by B cells and Th17 differentiation in SLE were evaluated in vitro . (frontiersin.org)
- Correspondingly, in vitro coculture studies showed that basophils obtained from patients with SLE promoted autoantibody production by SLE B cells and promoted Th17 differentiation from SLE naïve CD4 + T cells. (frontiersin.org)
- The main topic of the research work is to establish the value of the flow - assisted analysis of in vitro-activated basophils for drug hypersensitivity diagnosis. (abebooks.fr)
- In this study, we used flow cytometry to determine the percentage of activated basophils that expressed the CD63 marker after in vitro stimulation by different betalactam antibiotics. (cun.es)
- To study the biology of these rare cells, the team of Prof. Kaufmann has developed a method to differentiate and massively expand murine basophils in vitro . (forschung3r.ch)
- The study of primary basophils is challenging owing to their low numbers and short life-span, as well as to the lack of suitable in-vitro cell-modeling systems and efficient differentiation protocols. (forschung3r.ch)
- In project No. 127-11 the research group of Thomas Kaufmann has developed a novel methodology whereby large numbers of mouse basophils can be differentiated in vitro. (forschung3r.ch)
- These progenitors can be induced in vitro to differentiate into mature basophils on demand and in well-nigh unlimited quantities (2). (forschung3r.ch)
- Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). (viamedica.pl)
- 13) using in vitro activation of basophils concluded that encapsulating rocuronium by sugammadex can prevent, but not stop, the activation by the NMBA, and the administration of sugammadex unlikely mitigates anaphylaxis. (thefreedictionary.com)
- To investigate the mechanisms of anaphylactoid reactions to radiocontrast media, in vitro mediator release induced by three iodinated contrast agents was examined using peripheral blood basophils and mast cells purified from human lung parenchyma, heart, and skin tissues. (elsevier.com)
- This population of MCs/basophils can be maintained ex vivo for at least 2 weeks, and a comparable population of cells can be generated in vitro from nongranulated hematopoietic CD3(-)/CD4(+)/CD117(-) progenitors. (edu.au)
- The phenotypes of peripheral basophils and the production of autoantibody and interleukin (IL)-17 in patients with SLE were determined by flow cytometry and enzyme-linked immunosorbent assay, and also their correlations were investigated by statistical analysis. (frontiersin.org)
- Basophil activation in unfractionated samples such as PBMC (note basophils are less dense the Ficoll) or whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. (openwetware.org)
- Purpose of Review: We review basophil testing by flow cytometry with an emphasis on advantages and disadvantages. (kcl.ac.uk)
- In the last decade, an even more accessible methodology, using flow cytometry, has been developed to enhance the ability to use basophils for both mechanistic and clinical diagnostics. (kcl.ac.uk)
- It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. (viamedica.pl)
Absolute Basophil Count1
- A white blood cell count (WBC) test may be necessary to find the absolute basophil count in some cases. (medicalnewstoday.com)
- The captured basophils were activated using an anti-FceRI antibody and the basophil identification marker CD203c and the activation marker CD63 were detected using fluorescence microscopy. (kth.se)
- Basophils did not upregulate CD63 after passage through a microfluidic chip, but there was a slight but significant activation after crosslinking of CD203c, which is the surface marker used for basophil-specific capture in miBAT, giving one potential factor for the background activation. (kth.se)
- In addition, changes in the expression of basophil surface's molecules are observed: some antigens appear de novo (CD63, CD107a), and other increase their expression (CD13, CD203c). (termedia.pl)
- The CD203c was found exclusively on basophils and mast cells. (termedia.pl)
- The aim of the study was to evaluate idiopathic and stimulated CD203c expression on basophils before and after twelve months of specific sublingual immunotherapy. (termedia.pl)
- Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. (pubmedcentralcanada.ca)
- Heparin blood basophil activation was similar through CD63 and CD203c. (pubmedcentralcanada.ca)
- These activated basophils express activation markers (CD69, CD203c, and CD13) and release IL-4, IL-8, and IL-13. (sciencemag.org)
- The surface expression of CD203c (basophil selection marker) and CD63 (activation marker) after activation by the bacterial peptide formyl-methionyl-leucyl-phenylalanine (fMLP) or anti-Fcε receptor I (FcεRI) antibody and the absolute number of basophils was investigated before and after hemodialysis with each of the dialyzers. (pubfacts.com)
- in fact, the lack of basophil CD203c-upregulating activity in the serum of patients with chronic urticaria correlates with a good clinical response to omalizumab . (thefreedictionary.com)
Histamine release from basophils1
- Ioxaglate (0.2-0.3 M), ioxithalamate (0.3-0.5 M), and to a lesser extent ioversol (0.3-0.5 M) induced histamine release from basophils in a concentration-dependent manner. (elsevier.com)
- Basophils arise and mature in bone marrow . (wikipedia.org)
- Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. (springer.com)
- Basophils are white blood cells from the bone marrow that play a role in keeping the immune system functioning correctly. (medicalnewstoday.com)
- Basophils are produced continually by stem cells in the red bone marrow and move about in an amoeboid fashion. (encyclopedia.com)
- Within hours of their release from the bone marrow , basophils migrate from the circulation to the barrier tissues (e.g., the skin and mucosa), where they synthesize and store histamine , a natural modulator of the inflammatory response . (britannica.com)
- This paper investigates the pathophysiological role and disease-modifying effects of PDE3 in mouse bone marrow-derived MCs (bmMCs), human LAD2- and HMC1 mast cell lines, human blood basophils, and peripheral blood-derived primary human MCs (HuMCs). (frontiersin.org)
- Results: Basophils became more abundant in bone marrow (BM) and spleens of 19-month-old mice compared with 4-month-old mice. (eur.nl)
- Generation of conditionally immortalized, basophil-committed progenitor cell lines from the bone marrow of mice. (forschung3r.ch)
- These basophil progenitor lines are generated from murine bone marrow (or fetal livers) using a significantly modified version of a protocol that has been published for the production of murine neutrophils/macrophages (2, 3). (forschung3r.ch)
- Basophils have protein receptors on their cell surface that bind IgE , an immunoglobulin involved in macroparasite defense and allergy . (wikipedia.org)
- If the person has an allergy, the basophils in their blood sample will activate specific molecules. (medicalnewstoday.com)
- This is highly relevant with respect to human allergy and autoimmunity because a possible modulation of B cell differentiation by basophils could point to new therapeutic targets. (jimmunol.org)
- Two effector cells involved in allergic disease are mast cells and basophils, where basophils are more readily available in blood and therefore of great interest when studying allergy. (kth.se)
- Human basophils have long been thought to play a major role in allergy. (jimmunol.org)
- Basophils have been associated with fatal asthma, acute and chronic allergy, and have been shown to play an exacerbating role in lupus nephritis. (worldallergy.org)
- The reduced sensitivity to the inhibitory effect of Na+ may contribute to basophil dysfunction in patients with respiratory allergy. (ersjournals.com)
- The basophil activation test is a particularly useful technique in the diagnosis of patients with IgE?mediated allergy to betalactams and allows the identification of 50% of patients. (cun.es)
- Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. (viamedica.pl)
- Using two different methods to induce PC differentiation, we found that human basophils enhance B cell proliferation, class switching, differentiation into PC, maturation of PC, and production of Igs, especially IgG. (jimmunol.org)
- Basophil supernatants enhanced the expression of the B cell markers CD23 and CD40, which are important for B cell differentiation into IgG-producing PC. (jimmunol.org)
- Thus, human basophils modulate B cell differentiation into Ab-producing PC. (jimmunol.org)
- Our data demonstrate that human basophils support PC differentiation in an IL-3- and IL-4-dependent way. (jimmunol.org)
- Reciprocal regulation of human basophil and eosinophil differentiation by separate t-cell-derived factors. (thefreedictionary.com)
- Therefore, we studied the influence of aging and aging-associated microbiota on basophil frequency and phenotype, and differentiation from basophil precursors. (eur.nl)
- Their differentiation into mature basophils is attained upon a shutdown of exogenous Hoxb8 expression. (forschung3r.ch)
- Basophils are also antigen-presenting cells (APCs) and promote the differentiation of naïve T cells into helper T cells. (stemexpress.com)
Expression on basophils2
- Here we investigated the cellular and molecular mechanisms underlying acquired resistance to Haemaphysalis longicornis ticks in mice and found that antibodies were required, as was IgFc receptor expression on basophils but not on mast cells. (jci.org)
- During the hemodialysis procedure, the low-flux membrane induced up-regulation of CD63 expression on basophils, while passage through the high-flux membrane did not significantly alter the responsiveness. (pubfacts.com)
- Recent studies in mice suggest that basophils may also regulate the behavior of T cells and mediate the magnitude of the secondary immune response. (wikipedia.org)
- To assess the requirement for basophil infiltration to acquired tick resistance, mice expressing the human diphtheria toxin receptor under the control of the mast cell protease 8 (Mcpt8) promoter were generated. (jci.org)
- Diphtheria toxin administration to these mice selectively ablated basophils. (jci.org)
- FcγR therefore function as a regulatory module, made of two subunits with antagonistic properties, that prevents IgG-induced and controls IgE-induced basophil activation in both mice and humans. (jimmunol.org)
- Contrasting with the in vivo roles of human basophils, which can be primarily documented with correlations, the in vivo roles of mouse basophils can be investigated experimentally in wild-type (wt) mice, the basophils of which were depleted by appropriate Abs, or in genetically-modified mice that lack basophils. (jimmunol.org)
- Also, IgG1-induced passive systemic anaphylaxis, which depends on FcγRIIIA ( 13 ), was observed in mast cell-deficient mice ( 14 ), but it was abrogated by basophil depletion ( 15 ). (jimmunol.org)
- It was, however, not abrogated in basophil-deficient mice ( 5 ). (jimmunol.org)
- This application protocol describes the flow cytometric analysis of basophils after spleen dissociation from healthy C57BL/6 mice. (miltenyibiotec.com)
- This study aimed to investigate the role of basophil activation in the development of SLE based on studies in patients with SLE and spontaneous lupus-prone MRL- lpr/lpr mice. (frontiersin.org)
- Finally, the effect of basophil depletion on the development of autoimmune disorders in spontaneous lupus-prone MRL- lpr/lpr mice was examined. (frontiersin.org)
- The decrease of peripheral basophils in patients with SLE might be due to their migration to lymph nodes post their activation mediated by (autoreactive) IgE as supported by their increased CD62L and CCR7 expressions and accumulation in the lymph nodes of MRL- lpr/lpr mice. (frontiersin.org)
- Furthermore, an increased activation of peripheral basophils was identified in MRL- lpr/lpr mice. (frontiersin.org)
- Importantly, basophil-depleted MRL- lpr/lpr mice exhibited an extended life span, improved renal function, and lower serum levels of autoantibodies and IL-17, while basophil-adoptive-transferred mice exhibited the opposite results. (frontiersin.org)
- Although some liver basophils from naive mice express GFP, Nb infection enhanced GFP expression and increased the number of tissue basophils. (rupress.org)
- Similar basophil GFP expression was found in infected Stat6 −/− mice. (rupress.org)
- Rag2 −/− mice reconstituted with CD4 T cells allowed significant basophil accumulation, indicating that CD4 T cells can direct both tissue migration of basophils and enhanced IL-4 production. (rupress.org)
- See commentary " Mice that "conditionally" lack basophils, AT LAST " on page 2648. (pubmedcentralcanada.ca)
- Mice show basophils to be key players in the prevention of sepsis. (speakingofresearch.com)
- Microbiota transfers from young and old mice to germ-free recipients revealed that CD11b tended to be lowered on splenic basophils by aging-associated microbiota. (eur.nl)
- Subsequently, we purified FcϵRIα+CD11c-CD117- BM-derived basophils and found that those from aged mice expressed lower levels of CD11b upon stimulation. (eur.nl)
- Higher frequencies of IL-4+ basophils were generated from basophil precursors of aged mice, which could be reproduced in basophils derived from germ-free recipients of aging-associated microbiota. (eur.nl)
- Furthermore, this study shows that aging-associated microbiota altered activation of BM-derived basophils in a similar fashion as observed in BM-derived basophils from aged mice. (eur.nl)
- Importantly, such basophil-committed cell lines can be generated from transgenic mice with embryonically lethal or severe phenotypes. (forschung3r.ch)
- Consequently, large numbers of mice have to be sacrificed to generate sufficient quantities of primary basophils for experimental purposes. (forschung3r.ch)
-  It used to be thought that basophils that have migrated from blood into their resident tissues ( connective tissue ) are known as mast cells, but this is no longer thought to be the case. (wikipedia.org)
- Basophils were discovered in 1879 by German physician Paul Ehrlich , who one year earlier had found a cell type present in tissues that he termed mastzellen (now mast cells). (wikipedia.org)
- Reference ranges for blood tests of white blood cells, comparing basophil amount (shown in violet) with other cells. (wikipedia.org)
- Recent evidence suggests that basophils are an important source of the cytokine , interleukin-4 , perhaps more important than T cells . (wikipedia.org)
- In Basophils and Mast Cells: Methods and Protocols , experts in this challenging field explore techniques to research these cells from the most practical point of view. (springer.com)
- Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future research into mast cells and basophils with the goal of aiding in the quest to shed more light on these fascinating cell types. (springer.com)
- Although they have an essential function in the immune system, basophils only make up a small percentage of the total number of white blood cells. (medicalnewstoday.com)
- Myeloproliferative disorders affect white blood cells and may also cause very high basophil levels. (medicalnewstoday.com)
- Mast cells were once thought to be basophils that migrated from blood into their resident tissues (connective tissue), but they are now known to be different types of cells. (wikipedia.org)
- When antibodies of the immunoglobulin E (IgE) class bind to specialized receptor molecules on basophils, the cells release their stores of inflammatory chemicals, including histamine, serotonin , and leukotrienes. (britannica.com)
- Basophils make up only a small portion of the number of white blood cells but are important parts of the body's immune response. (medlineplus.gov)
- A diagram of white blood cells, including basophils. (wisegeek.com)
- Acting on the signal of basophils, neutrophils immediately respond by releasing enzymes that break down bacteria, foreign cells, and other microscopic threats. (wisegeek.com)
- The following scenario illustrates how the immune system uses basophils and other white blood cells when presented with a perceived threat. (wisegeek.com)
- Furthermore, murine basophils or their mediators enhance memory responses and plasma cell (PC) survival, suggesting that they directly modulate the function of B cells. (jimmunol.org)
- Therefore, the interaction between human B cells and basophils and the mechanism underlying this interaction were investigated in detail. (jimmunol.org)
- Basophils also express several surface markers that stimulate surrounding cells, for example CD62L ( 10 ), CD40L ( 11 ), OX40-L ( 12 ), and receptor activator of NF-κB ligand ( 13 , 14 ). (jimmunol.org)
- Thus, basophils are well equipped to react to a wide range of signals, and to influence the functions of other cells such as B cells. (jimmunol.org)
- Both B cells and basophils are present in the blood and the lymphoid organs ( 20 ), thereby facilitating contact between the two cell types. (jimmunol.org)
- In the current study, we therefore investigated whether human basophils and B cells interact and which mechanisms underlie this interaction. (jimmunol.org)
- The purpose of this study is to evaluate for changes in the proteins produced in white blood cells (basophils) in patients with chronic hives who are treated with and respond to omalizumab. (clinicaltrials.gov)
- However, the common scenario for severe allergic reactions are those mediated by the antibody IgE in conjunction with mast cells and basophils , following activation of both the innate and adaptive immune systems. (thefreedictionary.com)
- Like mouse ( 16 ) and human ( 17 ) mast cells, mouse and human basophils express not only IgE receptors, but also IgG receptors. (jimmunol.org)
- Eosinophils, mast cells, and basophils all were first recognized and described by Paul Ehrlich in the late 19th century. (worldallergy.org)
- There has been a steady and continued research effort directed to understanding both the function and the role of mast cells and basophils ever since these cells were first described at the end of the nineteenth century. (textbookx.com)
- The one characteristic that unites all types of mast cells and basophils appears to be the possession of the high affinity membrane receptor for immunoglobulin E, through which the cells may be stimulated. (textbookx.com)
- This volume begins with a review of the origin and development of mast cells and basophils. (textbookx.com)
- It continues by reviewing our knowledge of the stimuli to mast cells and basophils and the biochemical mechanisms which link the signal generated by stimulus to the response machinery of the cell. (textbookx.com)
- Further chapters deal with the drug control of mast cell and basophil responses, the heterogeneity of the cells' functions and the mediators' release by the cells, along with the morphological aspects of the cells' function. (textbookx.com)
- This book is completed by a selection of examples which examine the role of the mast cells or basophils in lung, skin and gastrointestinal tract. (textbookx.com)
- This book aims to provide an up-to-date account of mast cell and basophil biology, together with an insight into the functional relevance of these cells in some tissue types. (textbookx.com)
- The aim of this present study was to analyse the in situ immunolocalisation of basophils and other relevant inflammatory cells in chronic allergic keratoconjunctivitis. (bmj.com)
- BB-1-positive basophil clusters were surrounded by T cells, B cells, IgE-positive cells and MBP-positive eosinophils. (bmj.com)
- Thus, infection with a parasite that induces a "Th2-type response" resulted in accumulation of tissue basophils, and these cells, stimulated by a non-FcR cross-linking mechanism, are a principal source of in vivo IL-4 production. (rupress.org)
- Phenotypic and electron microscopic examination indicated that the IL-4-producing cell population was enriched in basophils and purified FcεRI + , c-kit − cells were shown to be excellent IL-4 producers ( 15 - 17 ). (rupress.org)
- Immune cells, including basophils, secrete the cytokine interleukin-4 (IL-4), which is known to play a role in the induction of asthma . (medicalxpress.com)
- Kubo and his colleagues showed that proteases could increase the expression of IL-4 in basophils but not in other types of immune cells. (medicalxpress.com)
- Following both IgE-mediated and substance P-mediated activation, PDE3i-pretreated basophils, LAD2 cells, and HuMCs, showed less degranulation than diluent controls, as measured by surface CD63 expression. (frontiersin.org)
- Basophils are difficult to distinguish from circulating mast cells. (vetstream.com)
- Mast cells and basophils : friends as well as foes in bronchial asthma? (nii.ac.jp)
- We show here that a seven-transmembrane receptor, CRTH2, which is preferentially expressed in T helper type 2 (Th2) cells, eosinophils, and basophils in humans, serves as the novel receptor for PGD 2 . (rupress.org)
- CRTH2 is intriguing in that it is selectively expressed in Th2 cells, T cytotoxic type 2 cells, eosinophils, and basophils ( 5 )( 6 )( 7 ). (rupress.org)
- Furthermore, CRTH2 but not DP induces migration of Th2 cells, eosinophils, and basophils in response to PGD 2 . (rupress.org)
- Our findings elucidated a novel function of PGD 2 on T cell responses, and CRTH2 is the first receptor that directly and selectively connects a major lipid mediator of activated mast cells with Th2 cells, eosinophils, and basophils. (rupress.org)
- Prostaglandin (PG) D 2 acts through both the DP 1 receptor, which is coupled to adenylyl cyclase, and the DP 2 receptor (chemoattractant receptor-homologous molecule expressed on Th2 cells), which is present on eosinophils, basophils, and Th2 cells and results in cell activation and migration. (aspetjournals.org)
- It is calculated by multiplying the percentage of basophils (in decimal form) by the total number of white blood cells. (cmlsupport.org.uk)
- Immunologic activation of purified human lung mast cells (HLMC) and basophils with anti-IgE induced histamine release but failed to elicit any changes in cAMP levels. (eurekamag.com)
- Basophil-like leukemia cells crucially contribute to CML development by providing an inflammatory chemokine, CCL3. (bloodjournal.org)
- Moreover, CCL3-expressing basophil-like leukemia cells were found to accumulate in CML BM and supported the predominant expansion of LICs therein. (bloodjournal.org)
- Basophils circulate in blood and mast cells stay in the connective tissue or in the mucosal tissue. (encognitive.com)
- The allergic patients show a statistically greater number of activated basophils than the control subjects, after the incubation of cells with all the betalactams at various concentrations. (cun.es)
- Basophils- similar to mast cells in skin, gastrointestinal, and respiratory tracts. (coursehero.com)
- They also inactivate substances released by basophils and mast cells during allergic reactions. (coursehero.com)
- First described by Paul Ehrlich in 1879, basophils have long been considered as circulating mast cells. (miltenyibiotec.com)
- Also, the amenability of the cells to further genetic manipulation, and the large number of cells that can be generated, render the model highly attractive for the study of molecular mechanisms (e.g., cell signaling pathways) in basophils. (forschung3r.ch)
- For specific scientific questions, this model could serve as an attractive alternative to primary cells in the study of basophil biology. (forschung3r.ch)
- An enriched population of cells with the phenotypic and functional characteristics of mature basophils is obtained within 6 days. (forschung3r.ch)
- The term "basophil" is used to designate one of the types of white blood cells (in a healthy person basophils constitute 0.5-1 percent of all the white blood cells) or one of the types of cells in the anterior lobe of the pituitary. (thefreedictionary.com)
- Despite these early reports it took almost 20 years until discoveries in hypersensitivity were linked to basophils and mast cells. (thefreedictionary.com)
- Three iodinated contrast agents, sodium and meglumine salts of ioxaglic acid, sodium and meglumine salts of ioxithalamic acid, and ioversol, were incubated with basophils purified from peripheral blood and human mast cells isolated and purified from different anatomical sites. (elsevier.com)
- Cross-linking of IgE by anti-IgE induced the release of leukotriene C 4 or prostaglandin D 2 from human basophils or mast cells. (elsevier.com)
- Mannitol, an osmotic stimulus, induced the release of histamine from human basophils, but to a lesser extent from mast cells. (elsevier.com)
- These results show that different contrast media can differ in their ability to release mediators from enriched preparations of human basophils and mast cells. (elsevier.com)
- The three contrast agents examined act on basophils and mast cells as incomplete secretagogues, causing the release of preformed mediators, but not the de novo synthesis of chemical mediators. (elsevier.com)
- the other types ( eosinophils , basophils , and mast cells ) have lower numbers. (wikipedia.org)
- CD4+ T cell , dendritic cell , B cell , mast cell , neutrophil , and basophil functions),  they are involved in the destruction of tumor cells,  and they promote the repair of damaged tissue. (wikipedia.org)
- This antibody then binds to basophils and a similar type of cell called mast cells. (soatechnology.net)
- Basophils account for less than three percent of your white blood cells. (soatechnology.net)
- Isolation and Purification of Human Mast Cells and Basophils. (kent.ac.uk)
- Basophils, eosinophils and mast cells were first recognized by Paul Ehrlich in the late 19th century. (elsevier.com)
- A population of metachromatic cells with mast cell (MC) and basophil features was identified recently in the peripheral blood of patients with several allergic disorders. (edu.au)
- When activated, basophils degranulate to release histamine , proteoglycans (e.g. heparin and chondroitin ), and proteolytic enzymes (e.g. elastase and lysophospholipase ). (wikipedia.org)
- Of these white blood cell types , basophils are designed to release histamine . (wisegeek.com)
- The primary function of basophils is to release histamine in response to allergens. (wisegeek.com)
- Basophils release histamine during allergic reactions. (soatechnology.net)
- Basophils also incite immediate hypersensitivity reactions in association with platelets , macrophages, and neutrophils. (britannica.com)
- We aimed to study the effect of blood-membrane interaction on human peripheral basophils and neutrophils in hemodialysis with high- and low-permeability polysulfone dialyzers. (pubfacts.com)
- Earlier studies reported that examination stress increases neutrophils, and platelets, and decreases eosinophil's, monocytes basophils , and lymphocytes. (thefreedictionary.com)
- Moreover, murine basophils support memory responses and the survival of in vivo differentiated PC ( 22 , 23 ). (jimmunol.org)
- In paper I, we developed a novel microfluidic immunoaffinity-based basophil activation test (miBAT) assay to investigate whether it was possible to capture and activate basophils from whole blood in a microfluidic chip. (kth.se)
- These data provide the first clear evidence, to our knowledge, that basophils play an essential and nonredundant role in antibody-mediated acquired immunity against ticks, which may suggest new strategies for controlling tick-borne diseases. (jci.org)
- Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-FcɛRI-β antibodies. (bmj.com)
- Basophils are also thought to play a role in causing the body to produce the antibody called immunoglobulin E (IgE). (soatechnology.net)
- Basophils are a type of white blood cell, which are vital components of the immune system. (medicalnewstoday.com)
- Basophil , type of white blood cell ( leukocyte ) that is characterized histologically by its ability to be stained by basic dyes and functionally by its role in mediating hypersensitivity reactions of the immune system . (britannica.com)
- How does the Immune System Use Basophils? (wisegeek.com)
- Basophils are part of the body's innate immune system that will be present at birth. (wisegeek.com)
- In conclusion, we have developed a novel microfluidic-based technique (miBAT) able to detect basophil activation (CD63-expression) using allergens in allergic patients. (kth.se)
- IgE recognition was clinically evaluated using the basophil activation test (BAT) for allergic patients and tolerant subjects. (mdpi.com)
- These results indicate that the fourth generation DeAns induce a higher level of basophil activation in allergic patients. (mdpi.com)
- Impact of thermal processing and the Maillard reaction on the basophil activation of hazelnut allergic patients. (biomedsearch.com)
- The incubation of basophils from healthy subjects with sera from allergic patients did not have a significant influence on the inhibitory effect of Na+. (ersjournals.com)
- The discordance between the results of specific IgE to β-lactams and the CD63 expression test on basophils was analysed in β-lactam allergic patients. (abebooks.fr)
- Diphtheria toxin-mediated basophil depletion before the second tick infestation resulted in loss of acquired tick resistance. (jci.org)
- For the patients with OAS, incubation of hazelnut proteins with glucose led to complete depletion of the stimulatory activity of the basophils. (biomedsearch.com)
- First, non-basophils are magnetically labeled for depletion over a MACS Column. (miltenyibiotec.com)
- Cytokine stimulation followed by IgE-independent (fMLP) basophil degranulation on the other hand showed a significantly increased CD63-expression compared to non-primed fMLP activation. (kth.se)
- Increase in basophil leucocytes (counts above 0.1 X 10^9/liters) is uncommon, but this is seen in myeloproliferative disorders such as chronic myeloid leukemia and polycythemia vera. (hubpages.com)
- In patients with chronic urticaria who respond clinically to omalizumab, the proteome of blood basophils will be measured at baseline (pre-treatment) and at weeks 6 and 13 (post-treatment). (clinicaltrials.gov)
- Histamine release reaction of basophils in chronic granulocytic leukaemia. (bmj.com)
- Basophils are a type of white blood cell . (wikipedia.org)
- Basophils contain anticoagulant heparin , which prevents blood from clotting too quickly. (wikipedia.org)
- High basophil levels may indicate an autoimmune condition or one of several types of blood disorder. (medicalnewstoday.com)
- Additionally, basophils play an integral role in preventing blood clotting. (medicalnewstoday.com)
- Doctors can use a complete blood count (CBC) to check a person's basophil levels. (medicalnewstoday.com)
- This test can help doctors get a better picture of the range of basophils in the blood. (medicalnewstoday.com)
- Blood tests may reveal basophil levels that are too high. (medicalnewstoday.com)
- Basophils contain anticoagulant heparin, which prevents blood from clotting too quickly. (wikipedia.org)
- The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. (ingentaconnect.com)
- Smoking and blood basophils. (bmj.com)
- The yield of captured basophils from whole blood was 64% at a capture flow rate of 3 µl/min. (kth.se)
- This is a basophil (white blood cell or granular leukocyte) which is moderately advanced. (sciencephoto.com)
- Basophils are only found in blood in healthy individuals (normally less than 1% of leukocytes), but are known to be rapidly recruited to inflamed tissues, where they can reach high densities (e.g. (worldallergy.org)
- The Human Blood Basophil. (annals.org)
- This monograph critically evaluates current research on human blood basophils. (annals.org)
- In addition, CRTH2, but not DP, mediates PGD 2 -dependent cell migration of blood eosinophils and basophils. (rupress.org)
- The total number of basophils in a sample of peripheral blood. (cmlsupport.org.uk)
- The article features a discussion on blood basophils and histamine levels in patients. (encognitive.com)
- In another series of experiments, LC50 calculated in whole blood (AP) was subtracted from LC50 calculated with basophils suspended in plasma from a nonatopic donor (HS) to determine the protective effect of soluble factors in blood of patients treated with SCIT. (pubmedcentralcanada.ca)
- Blood basophil concentration was marginally reduced by SCIT. (pubmedcentralcanada.ca)
- In addition to the blood basophils' attractiveness as surrogate marker for mast cell responses, it is a leukocyte that is recruited from blood to inflamed tissue in a type I allergic response [ 13 , 14 ]. (pubmedcentralcanada.ca)
- The team carried out several analyses on mouse models of sepsis, including full blood counts and cell population analysis, and found that a particular immune cell appears first in the fight against infection: the basophil. (speakingofresearch.com)
- For 50 years, peripheral blood basophils have been used as tools to study these diseases. (kcl.ac.uk)
- Basophils constitute less than 1% of the leukocyte population in peripheral blood. (forschung3r.ch)
- In some cases, having too many basophils can result from certain blood cancers. (soatechnology.net)
- You should have 0 to 300 basophils per microliter of blood. (soatechnology.net)
- Blood testing is the only way to discover whether your basophils are abnormal. (soatechnology.net)
- Finally, many patients with acquired immunodeficiency syndrome have HIV-1-infected MCs/basophils in their peripheral blood. (edu.au)
- Basophils are a type of white blood cell that plays a role in certain allergic reactions. (cancer.ca)
- Peripheral blood basophils are isolated from whole peripheral blood in a multi-step process. (stemexpress.com)
- Note the dark grains inside the cellular fluid (cytoplasm) of this basophil. (medlineplus.gov)
- However, OIT did not significantly suppress this response in basophils stimulated by the distinct fMLP receptor pathway. (nih.gov)
- Human basophils also express minute amounts of the GPI-anchored low-affinity IgG receptor FcγRIIIB ( 22 ), whose biological properties are unclear. (jimmunol.org)
- Several investigators have reported that levels of SYK may correlate with basophil responsiveness and that SYK is degraded as a consequence of receptor-induced anergy. (openwetware.org)
- This compound elicited DP 2 receptor-mediated CD11b expression in human basophils and eosinophils and induced actin polymerization and migration in eosinophils with a potency about the same as that of PGD 2 . (aspetjournals.org)
- Circulating IgD bound to basophils through a calcium-mobilizing receptor that induced antimicrobial, opsonizing, inflammatory and B cell-stimulating factors, including cathelicidin, interleukin 1 (IL-1), IL-4 and B cell-activating factor (BAFF), after IgD crosslinking. (lu.se)
- Human basophils may not undergo modulation by DC-SIGN and mannose receptor-targeting immunotherapies due to absence of receptors. (ovid.com)
- Upon receptor cross-linking by binding of antigen-IgE complexes, basophils degranulate and thereby release inflammatory mediators such as histamine and leukotriene. (miltenyibiotec.com)
- The aim of this study was to evaluate whether Na+ exerts its inhibitory effect on basophil histamine release induced by immunoglobulin E (IgE)-dependent (anti-IgE) and IgE-independent (N-formyl-methionyl-leucyl-phenylalanine (FMLP), interleukin-3 (IL-3)) stimuli in patients with allergic rhinitis (n = 24) and allergic bronchial asthma (n = 10). (ersjournals.com)
- The expression of CD63 in basophils following activation by fMLP was significantly higher in the patient group compared with that in healthy controls, but no differences were observed after activation by anti-FcεRI. (pubfacts.com)
- Human basophils produce many different mediators upon activation and/or during degranulation. (jimmunol.org)
- Furthermore, they are able to respond to various mediators because of their expression of different receptors for cytokines, TLR ligands ( 15 , 16 ), thymic stromal lymphopoietin ( 17 ), and IgG (FcγRIIa/IIb) ( 18 ) and IgE (FcεRIα) ( 19 ), facilitating Ag-specific basophil responses. (jimmunol.org)
- Basophils promote inflammatory and allergic (immediate and delayed hypersensitivity) reactions by release of mediators such as histamine, heparin, serotonin and hyaluronic acid. (vetstream.com)
- In this article, learn more about the function of basophils and what abnormal basophil levels mean. (medicalnewstoday.com)
- References of basophilia in ferrets have not been found in the scientific literature, so abnormal basophil counts are believed to be rare or very rare in ferrets. (vetstream.com)
- Mature Hoxb8 basophils are end-differentiated, have lobulated nuclei, a granular appearance, and express a number of cell-type-specific surface proteins (Fc□RI high CD11b + ckit neg Gr1 neg IL5R neg CCR3 neg ). (forschung3r.ch)
- In paper III, we studied basophil adhesion and activation to better understand both basophil function as well as the effect that basophil capture and stimulation in a microfluidic chip has on the cell. (kth.se)
- 2004) Leukocyte immunoglobulin-like receptors: novel innate receptors for human basophil activation and inhibition. (miltenyibiotec.com)
- The behaviour of atopics and healthy subjects was different and not related to the basophil responsiveness to activating signals. (ersjournals.com)
- Human basophils are known to play a role in Th2 responses by producing IL-4 ( 21 ), and could theoretically support IgG and IgE production. (jimmunol.org)
- With this, we provide an in-depth insight into the impact and mechanism by which human basophils modulate B cell functions in the healthy situation. (jimmunol.org)
- Besides high-affinity IgE receptors (FcεRI), human basophils express activating (FcγRIIA) and inhibitory (FcγRIIB) low-affinity IgG receptors. (jimmunol.org)
- and 6) that IL-3 upregulates the expression of both activating and inhibitory FcγR on human basophils from normal donors, but further enhances FcγRIIB-dependent inhibition. (jimmunol.org)
- Mouse basophils express FcεRI that are assumed to trigger similar signals as human basophil FcεRI. (jimmunol.org)
- IgG were long ago reported to bind to human basophils ( 18 ) on which two low-affinity IgG receptors were subsequently identified: FcγRIIA and FcγRIIB ( 19 , 20 ). (jimmunol.org)
- However some questions still remain regarding the relevance of these models to human basophil immunobiology. (worldallergy.org)
- Human basophils have also been demonstrated to be IL-4 producers ( 18 - 22 ). (rupress.org)
- Human basophil degranulation in schistosomiasis. (eurekamag.com)
- A human basophil degranulation test was applied with 3 dilutions of Schistosoma mansoni antigen (1, 10 and 100 mu g/ml). (eurekamag.com)
- Compared to Na-ASP-2, the chimeric fusion protein efficiently reduced the release of histamine in human basophils sensitized with anti-Na-ASP-2 IgE obtained from individuals living in a hookworm-endemic area. (nih.gov)
- We studied the histamine release reaction of leukaemic basophils in 10 patients and found a physiological response such as that previously reported with normal human basophils. (bmj.com)
- Unexpectedly, they observed that resting human basophils are activated and not suppressed in the presence of T regs . (sciencemag.org)
- Basophils were isolated from human PBMCs using the Diamond Basophil Isolation Kit and two MS Columns. (miltenyibiotec.com)
- 2005) Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. (miltenyibiotec.com)
- However, they did not induce the de novo synthesis of leukotriene C 4 or prostaglandin D 2 from human basophils or any type of mast cell examined. (elsevier.com)
- Both populations of MCs/basophils are susceptible to an M-tropic strain of human immunodeficiency virus 1 (HIV-1). (edu.au)
Fact that basophils2
- B cell-basophil interaction might also impact the pathogenesis of autoimmune diseases as indicated by the fact that basophils are differently activated in systemic lupus erythematosus (SLE) depending on the disease severity ( 10 ). (jimmunol.org)
- This novel concept combined with the fact that basophils selectively infiltrate allergic lesion sites has sparked greater interest in this once overlooked immune cell, both in adaptive as well as in innate immunity. (elsevier.com)
Occurrence of basophils1
- Wolf-Jurgensen, 1965, 1967), the description of CBH focused renewed attention on the occurrence of basophils in delayed-onset immunologic reactions, particularly in the guinea pig. (springer.com)
Activation of Basophils2
- Clinical immunology Specific sublingual immunotherapy decreases spontaneous and stimulated basophils degranulation. (termedia.pl)
- Significantly higher levels of MCHC, WBCs, monocytes, and basophils were observed in group 1, the younger birds. (thefreedictionary.com)
- Summary: A variety of studies have begun to establish both precise methods and their clinical relevance for disease diagnosis, but there remain some important questions on how to take optimal advantage of the behaviours of basophils. (kcl.ac.uk)
- IgG receptors (FcγR) were also found on mouse basophils, but not identified. (jimmunol.org)
- A total of five QTL for basophil were found, among which the QTL on SSC13 was resided closely with the lymphocytes QTL (Table 3). (thefreedictionary.com)
- The decreased numbers and an increased activation of peripheral basophils were found to be correlated with increased autoantibody production and disease activity in patients with SLE. (frontiersin.org)
- Basophils appear in many specific kinds of inflammatory reactions, particularly those that cause allergic symptoms. (wikipedia.org)