Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.
An in vitro test used in the diagnosis of allergies including drug hypersensitivity. The allergen is added to the patient's white blood cells and the subsequent histamine release is measured.
The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).
Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.
Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
Infections with nematodes of the order STRONGYLIDA.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
A genus of intestinal nematode parasites belonging to the superfamily HELIGMOSOMATOIDEA, which commonly occurs in rats but has been experimentally transmitted to other rodents and rabbits. Infection is usually through the skin.
Immunosuppression by the administration of increasing doses of antigen. Though the exact mechanism is not clear, the therapy results in an increase in serum levels of allergen-specific IMMUNOGLOBULIN G, suppression of specific IgE, and an increase in suppressor T-cell activity.
Allergic reaction to peanuts that is triggered by the immune system.
The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.
A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.
Agents that are used to treat allergic reactions. Most of these drugs act by preventing the release of inflammatory mediators or inhibiting the actions of released mediators on their target cells. (From AMA Drug Evaluations Annual, 1994, p475)
A plant genus of the family BETULACEAE. The tree has smooth, resinous, varicolored or white bark, marked by horizontal pores (lenticels), which usually peels horizontally in thin sheets.
Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
A vascular reaction of the skin characterized by erythema and wheal formation due to localized increase of vascular permeability. The causative mechanism may be allergy, infection, or stress.
Hypersensitivity reactions which occur within minutes of exposure to challenging antigen due to the release of histamine which follows the antigen-antibody reaction and causes smooth muscle contraction and increased vascular permeability.
Substances found in PLANTS that have antigenic activity.
The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.
CCR receptors with specificity for CHEMOKINE CCL11 and a variety of other CC CHEMOKINES. They are expressed at high levels in T-LYMPHOCYTES; EOSINOPHILS; BASOPHILS; and MAST CELLS.
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
An enzyme that catalyzes the hydrolysis of a single fatty acid ester bond in lysoglycerophosphatidates with the formation of glyceryl phosphatidates and a fatty acid. EC 3.1.1.5.
Immunologically mediated adverse reactions to medicinal substances used legally or illegally.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
An optical disk storage system for computers on which data can be read or from which data can be retrieved but not entered or modified. A CD-ROM unit is almost identical to the compact disk playback device for home use.
A genus of parasitic nematodes found throughout the rain-forest areas of the Sudan and the basin of the Congo. L. loa inhabits the subcutaneous tissues, which it traverses freely.
The anterior glandular lobe of the pituitary gland, also known as the adenohypophysis. It secretes the ADENOHYPOPHYSEAL HORMONES that regulate vital functions such as GROWTH; METABOLISM; and REPRODUCTION.
Relatively permanent change in behavior that is the result of past experience or practice. The concept includes the acquisition of knowledge.
A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.
Condensed areas of cellular material that may be bounded by a membrane.
An arthropod subclass (Xiphosura) comprising the North American (Limulus) and Asiatic (Tachypleus) genera of horseshoe crabs.
A syndrome characterized by persistent or recurrent fatigue, diffuse musculoskeletal pain, sleep disturbances, and subjective cognitive impairment of 6 months duration or longer. Symptoms are not caused by ongoing exertion; are not relieved by rest; and result in a substantial reduction of previous levels of occupational, educational, social, or personal activities. Minor alterations of immune, neuroendocrine, and autonomic function may be associated with this syndrome. There is also considerable overlap between this condition and FIBROMYALGIA. (From Semin Neurol 1998;18(2):237-42; Ann Intern Med 1994 Dec 15;121(12): 953-9)
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
An endogenous GAMMARETROVIRUS from the germ line of mice but isolated from humans. It has close similarity to xenotropic MURINE LEUKEMIA VIRUS.
A condition of low alertness or cognitive impairment, usually associated with prolonged mental activities or stress.
The number of times the HEART VENTRICLES contract per unit of time, usually per minute.

Evidence for the involvement of IgE-basophil system in acute serum sickness. (1/982)

The role of the basophils in acute serum sickness of rabbits was examined by monitoring daily the absolute number of basophils before, during and after the disease period. After antigen (bovine serum albumin, BSA) elimination, levels of serum IgE and in vitro basophil degranulation in the presence of BSA were determined. The results showed that the onset of glomerular lesions depends upon the simultaneous occurrence of circulating immune complexes greater than 19 S and of an in vivo basophil depletion--probably equivalent to degranulation--reaching 70% of the pre-disease number. Post-disease antigen-dependent in vitro degranulation of the basophils and levels of serum IgE anti BSA did not prove to be good indexes of basophil sensitization. Our data suggest that basophils are instrumental at early stages of the deposition of immune complexes, most probably through their sensitization by membrane-bound IgE antibodies.  (+info)

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. (2/982)

In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results.  (+info)

Extracellular signal-regulated kinases regulate leukotriene C4 generation, but not histamine release or IL-4 production from human basophils. (3/982)

Human basophils secrete histamine and leukotriene C4 (LTC4) in response to various stimuli, such as Ag and the bacterial product, FMLP. IgE-mediated stimulation also results in IL-4 secretion. However, the mechanisms of these three classes of secretion are unknown in human basophils. The activation of extracellular signal-regulated kinases (ERKs; ERK-1 and ERK-2) during IgE- and FMLP-mediated stimulation of human basophils was examined. Following FMLP stimulation, histamine release preceded phosphorylation of ERKs, whereas phosphorylation of cytosolic phospholipase A2 (cPLA2), and arachidonic acid (AA) and LTC4 release followed phosphorylation of ERKs. The phosphorylation of ERKs was transient, decreasing to baseline levels after 15 min. PD98059 (MEK inhibitor) inhibited the phosphorylation of ERKs and cPLA2 without inhibition of several other tyrosine phosphorylation events, including phosphorylation of p38 MAPK. PD98059 also inhibited LTC4 generation (IC50 = approximately 2 microM), but not histamine release. Stimulation with anti-IgE Ab resulted in the phosphorylation of ERKs, which was kinetically similar to both histamine and LTC4 release and decreased toward resting levels by 30 min. Similar to FMLP, PD98059 inhibited anti-IgE-mediated LTC4 release (IC50, approximately 2 microM), with only a modest effect on histamine release and IL-4 production at higher concentrations. Taken together, these results suggest that ERKs might selectively regulate the pathway leading to LTC4 generation by phosphorylating cPLA2, but not histamine release or IL-4 production, in human basophils.  (+info)

Down-regulation of human basophil IgE and FC epsilon RI alpha surface densities and mediator release by anti-IgE-infusions is reversible in vitro and in vivo. (4/982)

Previously, infusions of an anti-IgE mAb (rhumAb-E25) in subjects decreased serum IgE levels, basophil IgE and FcepsilonRIalpha surface density, and polyclonal anti-IgE and Ag-induced basophil histamine release responses. We hypothesized that these effects would be reversed in vivo by discontinuation of infusions and in vitro by exposing basophils to IgE. Subjects received rhumAb-E25 biweekly for 46 wk. Blood samples taken 0-52 wk after rhumAb-E25 were analyzed for serum IgE and basophil expression of IgE, FcepsilonRIalpha, and CD32. Basophil numbers were unaffected by infusions. Eight weeks after infusions, free IgE levels rose in vivo but did not reach baseline. Basophil IgE and FcepsilonRIalpha rose in parallel with free IgE while CD32 was stable. FcepsilonRI densities, measured by acid elution, returned to 80% of baseline, whereas histamine release responses returned to baseline. Basophils cultured with or without IgE or IgG were analyzed for expression of IgE, FcepsilonRIalpha, and CD32. By 7 days with IgE, expression of IgE and FcepsilonRIalpha rose significantly, whereas cultures without IgE declined. IgE culture did not effect CD32. IgG culture did not effect expression of any marker. The present results strongly suggest that free IgE levels regulate FcepsilonRIalpha expression on basophils.  (+info)

Adhesive explant culture of allergic nasal mucosa: effect of emedastine difumarate, an anti-allergic drug. (5/982)

Allergic reaction of the nose comprises of an immediate and a late reaction. To evaluate nasal allergic reactions, many experiments have been performed by investigators. In this study, we performed a new tissue culture technique (adhesive explant culture) to analyze the migration of cells into the culture medium from the cultured allergic nasal mucosa in response to an allergen. Basophilic cells (mast cells and basophils) and eosinophils, which were released into the culture medium after the allergen challenge, were evaluated by the analysis of histamine and eosinophil cationic protein (ECP) content in the culture medium. Histamine and basophilic cells in the culture medium were more abundant in the immediate phase (within 30 min) after challenge than in the late phase (from 30 min to 10 hr). On the other hand, ECP and eosinophils in the culture medium were more abundant in the late phase than in the immediate phase. The increase of histamine content in both phases were not inhibited by pre-treatment of emedastine difumarate (EME), an anti-allergic drug. However, the increase of ECP in the late phase was inhibited by pre-treatment with EME. Moreover, the number of EG2-positive cells was also decreased by pre-treatment with EME. These results suggest that EME might lower the activation of eosinophils in the late phase of the allergic reaction. The present study also indicates that this adhesive explant culture system is useful model for studying the cellular allergic responses to drugs ex vivo.  (+info)

The effect of processing on inflammatory markers in induced sputum. (6/982)

The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from healthy and atopic asthmatic subjects were treated with either DTE or phosphate-buffered saline (PBS) at 22 or 37 degrees C and compared for cell counts and concentrations of histamine, tryptase, eosinophil cationic protein (ECP), free interleukin (IL)-8, immunoglobulin (Ig)A, IL-8/IgA complexes and secretory component (SC). In addition, the influence of DTE on in vitro mediator release from blood eosinophils, basophils and bronchoalveolar lavage (BAL) mast cells was studied. Processing with DTE improved cytospin quality and increased the cell yield and measurable ECP, tryptase, IgA and SC, but reduced levels of histamine in PBS-treated samples and had no effect on IL-8. Cell counts or mediator levels were similar when sputum was processed at 22 or 37 degrees C, even though DTE induced blood basophils and BAL mast cells to release histamine at 37 degrees C. In spiking experiments, recovery of added ECP, tryptase, total IL-8 and histamine from sputum was similar in DTE- and PBS-processed sputum, but reduced for free IL-8 in PBS-treated samples. In conclusion, dithioerythritol improves cell and mediator recovery without causing cell activation when sputum processing is conducted at room temperature. The extent of recovery depends on the mediator studied.  (+info)

Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway: downregulation by sodium. (7/982)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 micrograms/ml). However, when extracellular Na+ was substituted iso-osmotically with N-methyl-d-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37 degrees for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM-CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mm, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+.  (+info)

Characterization of mast cell-committed progenitors present in human umbilical cord blood. (8/982)

Human mast cells are derived from CD34(+) hematopoietic cells present in cord blood, bone marrow, and peripheral blood. However, little is known about the properties of the CD34(+) cells. We demonstrated here that mast cell progenitors that have distinct phenotypes from other hematopoietic cell types are present in cord blood by culturing single, sorted CD34(+) cells in 96-well plates or unsorted cells in methylcellulose. The CD34(+) mast cell-committed progenitors often expressed CD38 and often lacked HLA-DR, whereas CD34(+) erythroid progenitors often expressed both CD38 and HLA-DR and CD34(+) granulocyte-macrophage progenitors often had CD33 and sometimes expressed CD38. We then cultured single cord blood-derived CD34(+)CD38(+) cells under conditions optimal for mast cells and three types of myeloid cells, ie, basophils, eosinophils, and macrophages. Of 1,200 CD34(+)CD38(+) cells, we were able to detect 13 pure mast cell colonies and 52 pure colonies consisting of either one of these three myeloid cell types. We found 17 colonies consisting of two of the three myeloid cell types, whereas only one colony consisted of mast cells and another cell type. These results indicate that human mast cells develop from progenitors that have unique phenotypes and that committed mast cell progenitors develop from multipotent hematopoietic cells through a pathway distinct from myeloid lineages including basophils, which have many similarities to mast cells.  (+info)

Basophils are a rare granulocyte population that has been associated with allergic and inflammatory responses. It is essential to understand the regulatory mechanisms by which basophils are kept in check, considering the impact of dysregulated basophil function on immune responses under different pathological conditions. Among immunoregulatory cells, CD4+CD25+FoxP3+ regulatory T cells (Tregs) are the key players that maintain immune tolerance. The mechanisms by which Tregs regulate and suppress diverse immune cell subsets have been studied extensively, but the impact of Tregs on basophil functions is not well understood. We report that human basophils are refractory to Treg-mediated suppression and found that Tregs stimulate resting basophils to induce the expression of activation markers including CD69, CD203c, and CD13 and the release of basophil cytokines including IL-13, IL-8, and IL-4. Mechanistically, Tregs could induce human basophil activation via IL-3 and STAT5 activation, whereas ...
RESUMEN. The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes.. Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes in ,0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils.. In addition to clinical history, skin prick test, and specific IgE determination, BAT can be a part of the diagnostic evaluation of patients with food-, insect venom-, and drug allergy and chronic urticaria. It may be helpful in determining the clinically relevant allergen.. Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is less stressful for the ...
Treatment of allergic disease by decreasing circulating IgE with anti-IgE Abs is currently under clinical study. Based on previous unrelated studies, it appeared likely that Fc(epsilon)RI expression on basophils and mast cells might also be regulated by levels of circulating IgE Ab. Therefore, the expression of IgE and Fc(epsilon)RI on human basophils was examined in 15 subjects receiving humanized anti-IgE mAb intravenously. Treatment with the anti-IgE mAb decreased free IgE levels to 1% of pretreatment levels and also resulted in a marked down-regulation of Fc(epsilon)RI on basophils. Median pretreatment densities of Fc(epsilon)RI were approximately 220,000 receptors per basophil and after 3 mo of treatment, the densities had decreased to a median of 8,300 receptors per basophil. Flow cytometric studies, conducted in parallel, showed similar results and also showed in a subset of 3 donors that receptors decreased with a t1/2 of approximately 3 days. The responsiveness of the cells to ...
The antigen-presenting abilities of basophils and their role in initiating a Th2 phenotype is a topic of current controversy. We aimed to determine whether human basophils can be induced to express MHC Class II and act as antigen presenting cells for T cell stimulation. Isolated human basophils were exposed to a panel of cytokines and TLR-ligands and assessed for MHC Class II expression. MHC Class II was expressed in up to 17% of isolated basophils following incubation with a combination of IL-3, IFN-γ and GM-CSF for 72 hours. Costimulatory molecules (CD80 and CD86) were expressed at very low levels after stimulation. Gene expression analysis of MHC Class II-positive basophils confirmed up-regulation of HLA-DR, HLA-DM, CD74 and Cathepsin S. However, MHC Class II expressing basophils were incapable of inducing antigen-specific T cell activation or proliferation. This is the first report of significant cytokine-induced MHC Class II up-regulation, at both RNA and protein level, in isolated human ...
The basophil activation test (BAT), in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry, is a rapid assay that is gaining popularity. Two markers are currently being evaluated for the BAT; CD63 and the lineage-specific CD203c. In a recent report, detection of CD203c after lysis with Saponin was shown to be superior to detection of CD63 after lysis with formic acid. We wanted to compare a) lysis with formic acid and lysis with Saponin, b) the response through CD203c and CD63, and c) the definition 10% activated cells above background with the probability binning metric T(χ) | 4, on sets of data generated with blood basophils stimulated with varying concentrations of anti-FcεRI antibody. Blood from volunteers was incubated with serial logarithmic dilutions of anti-FcεRI and subsequently with antibodies to CD203c PE and CD63 FITC. Sets of samples set up in parallel were lysed with either Saponin based Whole Blood Lysing reagent or
TY - JOUR. T1 - Cyclosporin A rapidly inhibits mediator release from human basophils presumably by interacting with cyclophilin. AU - Cirillo, Raffaele. AU - Triggiani, Massimo. AU - Siri, Lorenzo. AU - Ciccarelli, Anna. AU - Pettit, George. AU - Condorelli, Mario. AU - Marone, Gianni. PY - 1990. Y1 - 1990. N2 - We have examined the effects of cyclosporin A (CsA) and a series of CsA analogs that bind with decreasing affinity to cyclophilin, to evaluate the involvement of this protein in the release of preformed (histamine) and de novo synthesized (peptide leukotriene C4; LTC4) mediators of inflammatory reactions from human basophils. CsA (8 to 800 nM) concentration-dependently inhibited (5 to 60%) histamine release from peripheral blood basophils challenged with anti-IgE. CsA was more potent (92.6 ± 1.8 vs 59.1 ± 4.5%; p , 0.001) and, at low concentrations, more effective when the channel-operated influx of Ca2+ as bypassed by the ionophore A23187 (IC40 = 24.1 ± 3.9 vs 105.5 ± 22.2 nM; p , ...
Basophils represent ,1% of circulating leukocytes. They play a crucial role during allergy and helminth-induced Th2 responses. However, recent data also suggest a contribution to the pathogenesis of autoimmune diseases. Basophils from patients with systemic lupus erythematosus show an activated phenotype, correlating to disease activity. Furthermore, murine basophils or their mediators enhance memory responses and plasma cell (PC) survival, suggesting that they directly modulate the function of B cells. This is highly relevant with respect to human allergy and autoimmunity because a possible modulation of B cell differentiation by basophils could point to new therapeutic targets. Therefore, the interaction between human B cells and basophils and the mechanism underlying this interaction were investigated in detail. Using two different methods to induce PC differentiation, we found that human basophils enhance B cell proliferation, class switching, differentiation into PC, maturation of PC, and ...
The inhibitory myeloid immunoglobulin receptor CD300a (IRp60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to Th2-type immunity and allergy. Basophils homogeneously expressed CD300a as well as the inhibitory receptor CD200R on their cell surface, and these expressions increased after anti-IgE stimulation. IgE-mediated basophil degranulation was also significantly inhibited by crosslinking of either CD200R or CD300a (by 90% and 50%, respectively). Inhibitory SHIP-1 phosphorylations were also induced by CD200R and CD300a, although they were not noticeably increased by IgE-dependent activation. We conclude that both CD200R and CD300a play a role in reducing IgE-mediated basophil function and may crucially govern the known differential ...
TY - JOUR. T1 - Heterogeneity of human basophils and mast cells in response to muscle relaxants. AU - Stellato, C.. AU - de Paulis, A.. AU - de Crescenzo, G.. AU - Tatangelo, F.. AU - Rickler, O.. AU - Marone, G.. PY - 1992/6. Y1 - 1992/6. N2 - We investigated the in vitro effects of increasing concentrations (10-5-10-3M) of four muscle relaxants (succinylcholine, d-tubocurarine, vecuronium and atracurium) on histamine release (HR) from human peripheral blood basophils and mast cells isolated from lung parenchyma (HLMC) and skin tissues (HSMC). Basophils released less than 5% of their histamine content when incubated with any one of the muscle relaxants. In contrast, mast cells showed a marked heterogeneity in their response. Succinylcholine did not induce HR from any type of mast cell, and only high concentrations of d-tubocurarine (10-3M) caused HR from HSMC and HLMC. Vecuronium concentration-dependently induced HR from HLMC and HSMC. Atracurium concentration-dependently caused marked HR from ...
Fingerprint Dive into the research topics of Loss of syk kinase during IgE-mediated stimulation of human basophils. Together they form a unique fingerprint. ...
Purpose of Review: We review basophil testing by flow cytometry with an emphasis on advantages and disadvantages. Recent Findings: There are many tools available to assess the presence and severity of allergic diseases in patients. For 50 years, peripheral blood basophils have been used as tools to study these diseases. It is a very accessible cell that binds IgE antibody and secretes the classical mediators responsible for the symptoms of allergic reactions. In the last decade, an even more accessible methodology, using flow cytometry, has been developed to enhance the ability to use basophils for both mechanistic and clinical diagnostics. Basophil testing has been included in diagnostics for different forms of allergies as well as to monitor disease status. Summary: A variety of studies have begun to establish both precise methods and their clinical relevance for disease diagnosis, but there remain some important questions on how to take optimal advantage of the behaviours of basophils. ...
TY - JOUR. T1 - Piceatannol is an effective inhibitor of IgE-mediated secretion from human basophils but is neither selective for this receptor nor acts on syk kinase at concentrations where mediator release inhibition occurs. AU - Miura, K.. AU - Lavens-Phillips, S.. AU - MacGlashan, D. W.. PY - 2001/12/1. Y1 - 2001/12/1. N2 - Background: Syk kinase is probably an early necessary tyrosine kinase involved in IgE-mediated secretion from human basophils. Causal testing of the role ofsyk kinase in the secretion requires a selective pharmacological agent. Piceatannol has previously been used to demonstrate the causal role of syk in secretion but its selectively has recently come into question. Objective: To determine whether piceatannol inhibits IgE-mediated signalling events in a manner consistent with its putative inhibitory effects on syk kinase and at concentrations relevant to its inhibition of mediator release. Methods: Human basophils were examined for the effects of piceatannol on mediator ...
Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled with histadine, proteolytic enzymes, leukotrienes, and proteoglycans. Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can be identified by expression of CD200R3 and FceRIa. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these cell types using standard staining techniques.) *Produced only in pregnancy by the developing embryo. B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology: CD-ROM and Guide. Oxford University Press. ISBN 978-0195151732. MedicalMnemonics.com: ...
TY - JOUR. T1 - Flow cytometric methods for the analysis of human basophil surface antigens and viability. AU - Bochner, Bruce S.. AU - McKelvey, Alicia A.. AU - Schleimer, Robert P.. AU - Hildreth, James. AU - MacGlashan, Donald W.. PY - 1989/12/20. Y1 - 1989/12/20. N2 - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.. AB - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low ...
TY - JOUR. T1 - Effects of the Th2-dominant milieu on allergic responses in der f 1-activated mouse basophils and mast cells. AU - Yi, Myung Hee. AU - Kim, Hyoung Pyo. AU - Jeong, Kyoung Yong. AU - Kim, Ju Yeong. AU - Lee, In Yong. AU - Yong, Tai Soon. N1 - Funding Information: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST; grant nos NRF-2012M3A9B8021806 and 2016R1A2B4016194). We are grateful to Min-Ji Song, UnHye Kwon (Yonsei Biomedical Research Institute), and the Yonsei-Carl Zeiss Advanced Imaging Center, Yonsei University College of Medicine, for technical assistance.. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Although basophils and mast cells share similar phenotypic and functional properties, little is known about the difference in the initial Th2 immune responses of these cells following exposure to proteolytic allergens. Here, we investigated the mechanisms of Th2-mediated immune responses in mouse bone marrow-derived ...
Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bland Altman plot and ROC curves. Patients (n = 18) starting subcutaneous SCIT for wasp allergy with an up dosing scheme of 7 to 11 weeks were enrolled. Heparinised blood samples were drawn at weeks 1-4, 7 and at the first maintenance visit. Basophils were stimulated at 7 log dilutions of V. vespula allergen for 15 min, and were stained with CD203c and CD63. Basophils were identified as CD203c+ leukocytes, and the proportion of CD63+ and CD203c+ cells were plotted against allergen concentration. A sigmoid curve was fitted to the points, and the allergen concentration at which half of the maximal activation was achieved, LC50,
Basophils arise and mature in bone marrow. When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic enzymes (e.g. elastase and lysophospholipase). They also secrete lipid mediators like leukotrienes (LTD-4), and several cytokines. Histamine and proteoglycans are pre-stored in the cells granules while the other secreted substances are newly generated. Each of these substances contributes to inflammation. Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. Interleukin-4 is considered one of the critical cytokines in the development of allergies and the production of IgE antibody by the immune system. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation.[13] The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called ...
Allergic diseases affect approximately 30% of adults and has an impact on both the individuals quality of life as well as an economic impact on society. Two effector cells involved in allergic disease are mast cells and basophils, where basophils are more readily available in blood and therefore of great interest when studying allergy. Basophils can be recruited into the tissue during inflammation originating from for example allergic reactions or parasite infections. Allergy diagnostics starts with evaluation of the patients medical history followed by in vivo and/or in vitro testing. All diagnostic tests have different advantages and disadvantages are chosen depending on the patient and the circumstances. In vivo tests include the gold standard of allergy diagnostics, which is the challenge tests, but also the commonly used skin prick test (SPT). Allergy diagnostics can also be done in vitro using allergen-specific IgE antibody assays and the basophil activation test (BAT). BAT is useful to ...
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JA Denburg, WE Wilson, J Bienenstock; Basophil production in myeloproliferative disorders: increases during acute blastic transformation of chronic myeloid leuk
The John Schroeder Lab focuses on understanding the role human basophils and mast cells play in allergic reactions, as it relates not only to their secretion of potent inflammatory mediators (e.g., histamine and leukotriene C4) but also to their production of pro-inflammatory cytokines. We have long utilized human cells rather than cell lines in order to address the parameters, signal transduction and pharmacological aspects underlying clinically relevant basophil and mast cell responses. As a result, the lab has established protocols for rapidly isolating large numbers of basophils at high purity from human blood and for growing culture-derived mast cells/basophils from human progenitor cells. A variety of assays and techniques are also in place for concurrently detecting cytokines and mediators following a wide range of stimuli. These have facilitated the in vitro testing of numerous anti-allergic drugs for inhibitory activity on basophil and mast cell activation. The lab also studie...s ...
Various allergenicity assays have been performed, amongst others T-cell polarisation assays based on cultures of PBMC from allergic and control patients. A cell culture system with rat basophilic leukaemia cells (RBL) has been set up using IgE-containing patients sera to assess the ability of the allergens to respond. Allergens, processed or not have been tested using this model. Furthermore, inhibition studies and basophil histamine release tests are performed in cooperation with partners of the EuroPrevall project.. The immunomodulatory effects of several strains of three Lactobacillus species are assessed. All strains were capable of inducing IL-1β, IL-10, IFN-γ and TNF-α. L. plantarum strains showed a higher induction capacity of IFN-γ and TNF-α compared to the L. acidophilus and L. casei/paracasei strains. A larger variation in effects between species compared to within-species was observed.. ...
Basophil (好塩基球 Kōenkikyū) is a minor character in Cells at Work!. He is a type of white blood cell, specifically Basophil. He is capable of triggering the release of histamines when encountering an antigen. He also can allure eosinophils and neutrophils when they are needed. His role is not...
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
Previous research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). However, uncertainti
Looking for Basophils? Find out information about Basophils. The following article is from The Great Soviet Encyclopedia . It might be outdated or ideologically biased. , cells whose protoplasm contains granules that... Explanation of Basophils
This is an open-label, single-center study of whether oral penicillin desensitization of healthy sensitive subjects results in allergen cross-desensitization of mast cells by skin testing and desensitization of peripheral blood basophils. The primary endpoint(s) will be PC3 prick skin test values to penicillin, aeroallergen(s) and codeine. Secondary endpoints will be levels of Syk in purified basophils determined by flow cytometry (mean fluorescence intensity) and basophil degranulation (% tryptase release) to anti-FcåRI and to calcium ionophore ...
A nice review about the early non-T-cell source of IL-4 from basophils that act as Th2-inducing antigen-presenting cells. Even though we have shown that during LN responses to alum-precipitated antigens IL-4 is dispensable for the early induction of Th2-features including B cell maturation and helper T cell differentiation more, it is important to keep in mind that the basophil-source of IL-4 may have implication during the clearance of helminth infections and the development/maintenance/ augmentation of allergic diseases by induction of IgE production, activation of mast cells and basophils, as well as eosinophilic inflammation. An important role for basophils is unquestionable for they have been shown (together with mast cells) to release large amounts of IL-4 and IL-13 after cross-linkage of their IgE receptor, Fce receptor I (FceRI), by complexes of antigen plus antigen-specific IgE. However, recent evidence has proposed a novel role in antigen presentation to CD4 T cells that could play ...
A total of 103 samples of sputum from 20 patients with bronchial asthma were examined for appearance of basophils and eosinophils. The results are as follows: 1) Basophilic leucocytes were found in the sputum of patients with bronchial asthma. 2) Basophils appeared in sputum during asthma attacks and disappeared during nonattack stages. 3) The number of basophils in sputum tended to increase at the end of attack stages. 4) High incidence of basophils in sputum was observed in asthmatic patients with low serum IgE levels. 5) Appearance of basophils in sputum was accompanied with that of eosinophils. 6) Appearance of basophils in sputum was related to the number of peripheral basophils, but not to the daily volume of sputum. These results suggest that basophils migrate from the blood stream into bronchial tissues and appear in sputum during attack stages of bronchial asthma ...
This new book examines the latest research developments in the field of basophil granulocytes, sometimes referred to as basophils, which are the least common of the granulocytes, representing about 0.01% to 0.3% of circulating white blood cells. The name comes from the fact that these leucocytes are basophilic, i.e., they are susceptible to staining by base dyes, as shown in the picture. Basophils contain large cytoplasmic granules which obscure the cell nucleus under the microscope. However, when unstained, the nucleus is visible and it usually has 2 lobes. The mast cell, a cell in tissues, has many similar characteristics ...
Basophil: Basophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by basic dyes and functionally by its role in mediating hypersensitivity reactions of the immune system. Basophils, along with eosinophils and neutrophils, constitute a group of white
The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. Metachromasia was more identifiable through better preservation of the granules. The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules ...
Basophils are a group of leukocytes soldiers of the immune system that protects our body from external influences. If the indicator is increa...
A change in morphology and behavior of a basophil resulting from exposure to a cytokine, chemokine, soluble factor, or to (at least in mammals) an antigen which the basophil has specifically bound via IgE bound to Fc-epsilonRI receptors, leading to the in…
II.E.001. Normal basophil. Blood film. The field contains a basophil and lymphocyte. The dark blue basophilic granules characteristically obscure the nucleus. The granules are relatively large and easily resolved with the light microscope. In this case some cytoplasmic basophilic granules were lost in film preparation and the cell is partially degranulated as a result of the trauma of preparation. This finding is common in blood films.. ...
Type We allergy is characterized by the development of an initial Th2-dependent allergen-specific IgE response, which is boosted upon subsequent allergen encounter. anti-CD154 only) during sensitization prevented the introduction of allergen-specific IgE, IgM, IgG, and IgA reactions compared to neglected, but sensitized mice. Nevertheless, co-stimulation blockade got no impact on founded IgE reactions in sensitized mice. Immediate type reactions as examined with a rat basophil leukemia cell mediator launch assay were just suppressed by early treatment however, BAY 61-3606 not by co-stimulation blockade after sensitization. CTLA4Ig provided alone didnt suppress both supplementary and major allergen-specific antibody responses. Allergen-specific T cell activation was suppressed in mice by early aswell as past due co-stimulation blockade recommending that IgE-responses in sensitized mice are 3rd party of T cell help. Our outcomes indicate that T cell suppression only without active immune system ...
Human HLA B27-restricted cytotoxic T lymphocytes (CTL) specific for the influenza A epitope NP383-391 use similar TCR alpha and beta chains, with two closely related J alpha segments used by six of nine CTL clones from three unrelated donors (Bowness et al., Eur J. Immunol. 1993. 23: 1417-1421). The role of TCR complementarity-determining region (CDR)3alpha residues 93 and 100-102 was examined by site-directed mutagenesis, following expression of the TCR alpha and beta extracellular domains from one clone as a TCR zeta fusion heterodimer in rat basophil leukemia (RBL) cells. For the first time we have measured direct binding of tetrameric HLA B*2705/NP383-391 complexes to transfected TCR. Independently peptide-pulsed antigen-presenting cells (APC) were used to induce TCR-mediated degranulation of RBL transfectants. Our results show a key role for the conserved TCRalpha CDR3 J alpha-encoded residue Y102 in recognition of HLA B27/NP383-391. Thus the Y102D mutation abolished both tetramer binding and
References of basophilia in ferrets have not been found in the scientific literature, so abnormal basophil counts are believed to be rare or very rare in ferrets ...
Define basophil: a basophilic substance or structure; especially : a white blood cell containing basophilic granules that is similar in function to a…
Fig 3.: Comparable to primary mouse basophils, the Hoxb8 variety is characterized by a relatively short half-life in culture, which was significantly prolonged after the addition of the cytokine interleukin-3 (IL-3). Cell viability (double negative annexin V/PI-population) was assessed by flow cytometry.. In summary, we have established a method to generate basophil-committed progenitor lines using Hoxb8. These progenitors can be induced in vitro to differentiate into mature basophils on a large scale, which, to the best of our knowledge, is a novel and unique attribute for a model of the kind. The here-described cell lines are genetically stable, can in principle be generated from any genetically modified strain of mice that survives up to embryonic stage E12, and can be easily handled in culture. Since large numbers of mice are required to isolate primary basophils, we would like to promote this cellular model as an alternative system to study specific aspects of basophil biology, and ...
Sato, S., 1975: Studies on supravital observation of basophils in bronchial asthma in immunological reaction part 1 changes of migration velocity of basophils by addition of anti immuno globulin
Many particles and molecules in the environment can trigger allergic asthma in susceptible individuals. The allergic response to some of these allergens results in lung inflammation that can lead to a narrowing of the airways and even severe respiratory difficulty. A research team led by Masato Kubo from the RIKEN Center for Integrative Medical Sciences has now identified that a type of immune cell called a basophil is responsible for initiating a cascade of events that leads to inflammation of the lung in mice after exposure to plant- and dust-mite-derived allergens.
Remove the 2 mL Cryotube with 200 μL of the 20x basophil media, thaw and add 1.8 mL of warm 37°C ) RPMI medium. Close the stopper and vortex it for 5 sec. This is the 2X basophil media to be used for incubation with the whole blood and for preparation of the egg or peanut allergen dilutions (see below ...
Purpose of review Mechanisms involved in the development of type 2 immunity are poorly defined. In addition progenitors that differentiate into mature basophils have recently been recognized. Summary The current review revisits basophils with the goal of providing insights into understanding unappreciated functions of basophils studies of human basophils have provided insights into understanding basophil […]. ...
Four subjects with clinical histories of milk-induced asthma were studied (three allergic to cows milk; one to soya milk). In each instance, skin prick tests, RAST (IgE and IgG4), the basophil histamine release, and serum precipitins, using appropri
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By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells. ...
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
TY - JOUR. T1 - CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections. AU - Bakocevic, Nadja. AU - Claser, Carla. AU - Yoshikawa, Soichiro. AU - Jones, Leigh Ann. AU - Chew, Samantha. AU - Goh, Chi Ching. AU - Malleret, Benoit. AU - Larbi, Anis. AU - Ginhoux, Florent. AU - de Lafaille, Maria Curotto. AU - Karasuyama, Hajime. AU - Renia, Laurent. AU - Ng, Lai Guan. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 2014/6. Y1 - 2014/6. N2 - Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45intCD49b+FcεRI+ cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of αIIbβ3 integrin. CD41 expression on basophils is upregulated after in vivo IL-3 treatment and during infection with Nippostrongylus brasiliensis (Nb). Moreover, CD41 can be used as a reliable marker for ...
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic ...
Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat basophilic leukaemia cell line RBL-2H3, and compared their properties with each other and with plasma-membrane vesicles. DRM fractions were isolated as sealed unilamellar vesicles of similar size (135-170 nm diameter), using either sucrose-density-gradient sedimentation or gel-filtration chromatography. Lipid rafts isolated using Brij-96 and Triton X-100 differed in density, protein content and the ...
Looking for online definition of basophil substance in the Medical Dictionary? basophil substance explanation free. What is basophil substance? Meaning of basophil substance medical term. What does basophil substance mean?
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Resistance to infestation by larval Amblyomma americanum or Rhipicephalus sanguineus ticks was transferred to naive guinea pigs with peritoneal exudate cells (PEC) or serum from donors immunized by prior infestation with homologous tick larvae. In the A. americanum system, PEC transfer induced 87% tick rejection, which was similar to the level of resistance in actively sensitized hosts. In the R. sanguineus system, PEC conferred resistance (39% rejection) that was weaker than in actively sensitized hosts (57% rejection). In both systems, immune serum conferred significant but weaker resistance (20 to 29% rejection). In actively sensitized hosts, resistance to each tick species was specific, but there was considerable cross-reactive resistance. Basophils dominated the 24-hr challenge feeding sites of A. americanum ticks in actively sensitized hosts (69% of the infiltrate) and recipients of sensitized PEC (69%). Mononuclear cells were dominant (69% of the infiltrate) in the challenged tissues of ...
TY - JOUR. T1 - A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells. AU - Barth, Erin E.. AU - Hallworth, Richard J.. AU - Nichols, Michael G.. PY - 2005/5. Y1 - 2005/5. N2 - Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint will necessarily depend on the characteristics of the damage induced by the agent. By using multiple biological probes, it is possible to get a more complete description of the type of damage induced. Photodamage was induced in rat basophilic leukemia cells by either 254-nm UVC light exposure or rose bengal photosensitization. Damage was measured by three quantitative assays employing fluorescent probes: calcein, to measure nonspecific esterase activity, propidium iodide (PI), to measure loss of plasma membrane integrity, rhodamine 123 ...
TY - JOUR. T1 - Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test. AU - Okamoto, Kaoru. AU - Kagami, Michiko. AU - Kawai, Manabu. AU - Mori, Yuji. AU - Yamawaki, Kazuo. AU - Nakajima, Yoichi. AU - Kondo, Yasuto. AU - Tsuge, Ikuya. PY - 2019/1. Y1 - 2019/1. UR - http://www.scopus.com/inward/record.url?scp=85058961080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85058961080&partnerID=8YFLogxK. U2 - 10.1016/j.alit.2018.08.001. DO - 10.1016/j.alit.2018.08.001. M3 - Letter. C2 - 30190098. AN - SCOPUS:85058961080. VL - 68. SP - 130. EP - 131. JO - Allergology International. JF - Allergology International. SN - 1323-8930. IS - 1. ER - ...
The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Belarus. The Basophil Activation Test (BAT) is a modern cellular method of allergy diagnosis, which due to its high efficiency has firmly taken its place in the diagnostic algorithm of food, pollen and drug allergy, and chronic urticaria. A promising direction of the BAT is to evaluate the effectiveness of specific immunotherapy, treatment with anti-IgE drugs, and the natural resolution of allergy. The current review presents the main approach of the BAT procedure and the efficacy of the use for the diagnosis for various types of allergies, as well as the rules for evaluating the diagnostic results. ...
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
This is a case report of anaphylaxis in which the basophil activation test (BAT) was used to identify the etiological agent. Although skin tests are considered the most effective methods for identifying anaphylactic triggers, the test itself presents a risk of inducing anaphylaxis. The BAT is advantageous because of its inherent lack of risk, high sensitivity and specificity to identify the suspected anaphylactic agents, and diagnostic accuracy comparable to conventional skin testing. Therefore, in the future, the BAT is likely to become the preferred test for the detection of allergens over conventional skin tests. ...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
Basophils possess membrane bound IgE molecules, and immunological activation leads to a secretory process with cell degranulation and histamine release. Heterologous anti IgE, concanavaline A, and phytohaemagglutinin are potent non-cytotoxic releasing agents. They operate by a mechanism similar to that of immunological activation. Heavy water is not a histamine releasing inducer but it increases histamine release of the cells. We studied the histamine release reaction of leukaemic basophils in 10 patients and found a physiological response such as that previously reported with normal human basophils.. ...
Mercuric chloride (HgCl2) is an industrial agent known to cause autoimmune disorders and induce IgE synthesis, which plays a crucial role in the manifestation of allergic diseases. In rodents, the immunomodulatory effects of HgCl2 have been shown to involve the enhancement of mast cell-derived IL-4 secretion, which facilitates both Th2-lymphocyte development and IgE production. In humans, rapid allergen-dependent release of IL-4 and the related cytokine IL-13 from histamine-containing cells occurs primarily in basophils, along with other proinflammatory mediators such as histamine and LTC4. In this study, we therefore investigated the effects of HgCl2 on the release of the above basophil mediators, either due to the compound alone or in conjunction with IgE-dependent stimulation. HgCl2 (10(-9) to 10(-6) M) did not induce mediator secretion alone but significantly enhanced the release of histamine, LTC4, IL-4, and IL-13 caused by anti-IgE. Higher concentrations of HgCl2 (10(-5) to 10(-3) M) ...
The aim of this study was to evaluate whether Na+ exerts its inhibitory effect on basophil histamine release induced by immunoglobulin E (IgE)-dependent (anti-IgE) and IgE-independent (N-formyl-methionyl-leucyl-phenylalanine (FMLP), interleukin-3 (IL-3)) stimuli in patients with allergic rhinitis (n = 24) and allergic bronchial asthma (n = 10). Peripheral blood leucocytes were stimulated with anti-IgE, FMLP and IL-3 in the presence of high and low Na+ concentrations, and histamine release was measured using a fluorometric method. In standard Na(+)-containing medium, spontaneous and stimulated histamine release was higher in allergic patients (n = 34) (both rhinitic and asthmatic) than in healthy subjects (n = 41). Na+ removal from extracellular medium and its isosmotic substitution with choline chloride or with N-methyl-D-glucamine led to a significant increase of anti-IgE-, FMLP- and IL-3-induced histamine release in normal subjects, but not in allergic patients. The increase in Na+ ...
Peachell P.T.; Macglashan D.W.Jr; Lichtenstein L.M.; Schleimer R.P., 1988: Regulation of human basophil and lung mast cell function by cyclic amp
2. People with leukemia often show high basophil counts on a blood test. Each of these substances contributes to inflammation. A complete blood count, or CBC, measures several components of your blood and can help diagnose a broad range of conditions, from anemia and to cancer. Basophil: A type of white blood cell (leukocyte) with coarse, bluish-black granules of uniform size within the cytoplasm. They release special enzymes to help protect your … [10] They are found in tissues where allergic reactions are occurring and probably contribute to the severity of these reactions. home/medterms medical dictionary a-z list / basophil definition. Medical BASOS acronym meaning defined here. This test can help doctors get a better picture of the range of basophils in the blood. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation. Basophils are instrumental to immune system function, so low levels may also signal that the ...
Parekh, A. B.; Penner, R.: Activation of store-operated calcium influx at resting InsP(3) levels by sensitization of the InsP(3) receptor in rat basophilic leukaemia cells. Journal of Physiology-London 489 (2), pp. 377 - 382 (1995 ...
Matsumoto K, Kouzaki H, Kikuoka H, et al. Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33[J]. Allergology International, 2018.
The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs. - Great Britain
The table below shows the top 200 pain related interactions that have been reported for Basophil Adenoma. They are ordered first by their pain relevance and then by number of times they were reported in Basophil Adenoma. Please click on the INT link to display more detailed information on each interaction. ...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
The conditions and cell sources for colony stimulating activity (CSA) production by nasal polyp epithelial scrapings were examined. Epithelial scrapings removed from patients were grown to confluence during 7 days as monolayers of epithelial cells in media supplemented with fetal calf serum (FCS) on collagen-coated microwell plates. Growth kinetics of nasal polyp epithelial cells (NPECs) were determined, and CSA in NPEC conditioned medium (CM) was assessed with density-gradient separated, nonadherent peripheral blood mononuclear cells in standard 14-day methylcellulose assays. Nasal polyp cultures in the presence of 5% or 15% FCS (vol/vol) demonstrated significantly more epithelial cell proliferation than cultures at 0% and 1% FCS. There were comparable metachromatic cell counts in polyp epithelial scrappings from allergic and nonallergic donors. Similarly, NPEC CM from allergic and nonallergic donors had equivalent CSA for basophil/mast cell (BMC) and eosinophil (EO) lineages, respectively. CSA ...
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Epithelial mast cells are generally present in the airways of patients with allergic asthma that are inadequately controlled. Airway mast cells (MCs) are critically involved in allergic airway inflammation and contribute directly to the main symptoms of allergic patients. Phosphodiesterase 3 (PDE3) tailors signaling of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are critical intracellular second messenger molecules in various signaling pathways. This paper investigates the pathophysiological role and disease-modifying effects of PDE3 in mouse bone marrow-derived MCs (bmMCs), human LAD2- and HMC1 mast cell lines, human blood basophils, and peripheral blood-derived primary human MCs (HuMCs). In a chronic house dust mite (HDM)-driven allergic airway inflammation mouse model, we observed that PDE3 deficiency or PDE3 inhibition (PDE3i) therapy reduced the numbers of epithelial MCs, when compared to control mice. Mouse bone marrow-derived MCs (bmMCs) and the ...
An allergic reaction to food is a two-step process.. Step 1:. The first time you are exposed to a food allergen, your immune system reacts as if the food were harmful and makes specific IgE antibodies to that allergen. The antibodies circulate through your blood and attach to mast cells and basophils. Mast cells are found in all body tissues, especially in areas of your body that are typical sites of allergic reactions. Those sites include your nose, throat, lungs, skin, and gastrointestinal (GI) tract. Basophils are found in your blood and also in tissues that have become inflamed due to an allergic reaction.. Step 2:. The next time you are exposed to the same food allergen, it binds to the IgE antibodies that are attached to the mast cells and basophils. The binding signals the cells to release massive amounts of chemicals such as histamine. Depending on the tissue in which they are released, these chemicals will cause you to have various symptoms of food allergy. The symptoms can range from ...
The ultrastructural study of the secretory cells type 1 and 2 confirmed the separate identities of two secretory cell types in the gut of female B. microplus. Secretory cell type 1 (s1) synthesized and secreted large, spherical, uniformly electrondense granules. Secretory cell type 2 (s2) synthesized smaller, irregularly shaped and more complex granules. Another cell type, the basophilic cell, was shown to be the reorganized basal remnant of secretory cell s2. A few of the basophilic cells retained remnant s2 granules within their cytoplasm. In these cells the reorganized cisternae of rough endoplasmic reticulum were arranged in whorls and parallel arrays. The cells synthesized granules with a different ultrastructure and position in the cell from the earlier granules. The new secretory material may be egg proteins which are released into the haemolymph, and transported to the ovary. Another secretory cell type with smaller spherical granules was seen in the gut caeca of only two female ticks ...
Gibbs, Bernhard F and Ennis, M. (2001) Isolation and Purification of Human Mast Cells and Basophils. In: Rogers, D. and Donnelly, Louise E., eds. Human Airway Inflammation: Sampling techniques and analytical protocols. The Humana Press Inc., pp. 161-176. ISBN 978-0-89603-923-0. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:4831) ...
Clone REA758 recognizes the human FcεRIα, the α subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one α-, one β-, and two disulphide-linked γ-chains, of which the α subunit binds IgE1. FcεRIα is expressed on mast and basophil cells, is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.Additional information: Clone REA758 displays negligible binding to Fc receptors. | Principat dAndorra
Current Research and Scholarly Interests The goals of Dr. Gallis laboratory are to understand the regulation of mast cell and basophil development and function, and to develop and use genetic approaches to elucidate the roles of these cells in health and disease. We study both the roles of mast cells, basophils, and IgE in normal physiology and host defense, e.g., in responses to parasites and in enhancing resistance to venoms, and also their roles in pathology, e.g., anaphylaxis, food allergy, and asthma, both in mice and humans. ...
Current Research and Scholarly Interests The goals of Dr. Gallis laboratory are to understand the regulation of mast cell and basophil development and function, and to develop and use genetic approaches to elucidate the roles of these cells in health and disease. We study both the roles of mast cells, basophils, and IgE in normal physiology and host defense, e.g., in responses to parasites and in enhancing resistance to venoms, and also their roles in pathology, e.g., anaphylaxis, food allergy, and asthma, both in mice and humans. ...
Fingerprint Dive into the research topics of Receptor-operated, but not voltage-operated, calcium channels are involved in basophil leucocyte activation and histamine release. Together they form a unique fingerprint. ...
Which of the following cells is found in the cartilaginous tissue of the body? (a) Mast cells (b) Basophils (c) Osteocytes (d) Chondrocytes
BCX 1470 methanesulfonate 1). Fig. 4 Behavior from the intestinal Mouse monoclonal to CD15.DW3 reacts with CD15 (3-FAL ), a 220 kDa carbohydrate structure, also called X-hapten. CD15 is expressed on greater than 95% of granulocytes including neutrophils and eosinophils and to a varying degree on monodytes, but not on lymphocytes or basophils. CD15 antigen is important for direct carbohydrate-carbohydrate interaction and plays a role in mediating phagocytosis, bactericidal activity and chemotaxis mucosal microcirculatory factors. a complete vascular thickness. b Perfused vascular thickness. c Percentage of perfused vessels. d Microvascular BCX 1470 methanesulfonate stream index. e Crimson bloodstream cell speed. f Heterogeneity stream index Fig. 5 Behavior from the intestinal serosal microcirculatory factors. a complete vascular denseness. b Perfused vascular denseness. c Percentage of perfused vessels. d Microvascular BCX 1470 methanesulfonate movement index. e Crimson bloodstream cell speed. f ...
Basophile Granulozyten sünd Zellen, de to de Bestanddeelen vunt Blood höört un to de Witten Bloodkörpers (Leukozyten) tellt warrt. De basophilen Granulozyten wiest tallrieke Granula op, de groff un nich regelmatig sünd und ünner annern Histamin un Heparin bargt. Int Differentialbloodbild maakt se blots en lütten Andeel vun 2 bit 6 % ut.[1] De basophilen Granulozyten stammt ut dat Knakenmark, se hebbt mit de eosinophilen Granulozyten en gemeesomen Vörlöper. Wassdomsfakters för de Basophilen sünd ünner annern Interleukin-3, Interleukin-5 un GM-CSF. Dat gifft Henwiesen dorop, dat sik de Basophilen un de Eosinophilen bit Riepen gegensietig kontrolleert. Ton Bispeel ünnerdrückt de transformeeren Wassdomsfakter TGF int Biwesen vun Interleukin-3 dat Differenzeren vun Eosinophilen un föddert dat vun de Basophilen. Se hebbt en Rezepter fört Immunglobulin E (IgE), wat to de Annahmföhrt, dat se en Rull speelt bi de Immunafwehr vunn Weert gegen Parasiten. Wenn jemehr Rezepters dör ...
Fingerprint Dive into the research topics of INKT cells are responsible for the apoptotic reduction of basophils that mediate Th2 immune responses elicited by papain in mice following γPGA stimulation. Together they form a unique fingerprint. ...
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/ typeof=CreativeWork http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/,Regulation of type 2 immunity by basophils, David Voehringer, (electronic resource),/a,,/span, - ,span property=offers typeOf=Offer,,span property=offeredBy typeof=Library ll:Library resource=http://link.library.deakin.edu.au/#Deakin%20University%20Library,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.deakin.edu.au/,Deakin University Library,/a,,/span,,/span,,/span,,/span,,/div ...
A basophil is a type of cell belonging to the white blood cell family. While only comprising a small percentage of the total white blood cells circulating in the blood (normal range: 0.4%-1%), they are the largest type of mature white blood cell. They are created in response to inflammation and infection like all white blood…
Echinacea pallida (EPAL), also known as pale purple coneflower, is a herbaceous flowering plant with immune-enhancement and antioxidative properties. The effect of EPAL on the reproductive performance, serum biochemistry and haematological parameters of rabbit does has been studied here. A total of 100, 21-week-old Grimaud rabbit does, were randomly assigned to two groups. One group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E), while the other was fed the basal diet without the supplementation (control group, C). The reproductive performance of the does was not affected by the treatment (P,0.05). The haematological parameters of pregnant rabbits showed that there was no interaction between gestation day and treatment. The EPAL supplementation induced a reduction (−47.3%) in the basophil cell rate (0.55% and 0.29%, for the control and treatment groups, respectively; P=0.049). The gestation day significantly affected most of the haematological parameters (P,0.05). ...
Some monocytes contain azurophilic granules much finer than those seen in lymphocytes. The nucleus is seldom round; usually it is deeply indented and lobulated (often bean-shaped). Its loose, delicate chromatin pattern is unique among the blood cells. Clumps of chromatin may be found scattered among larger, chromatin-poor areas. Nucleoli are rarely present. 55 4 · Individual Cells Fig. 13 a - d a b c d IV 56 Chapter IV · Blood and Bone Marrow Fig. In absolute terms, mitoses are more frequent than in the myeloblasts (Fig. 7 d - h). 39 4 · Individual Cells Fig. 7 a - d a b c d IV 40 Chapter IV · Blood and Bone Marrow Fig. 7 e - h IV e f g h 41 4 · Individual Cells Neutrophilic Myelocytes, Metamyelocytes, Band and Segmented Forms, also Eosinophilic and Basophilic Granulocytes and Tissue Basophils (Fig. 8 a - h) The offspring of the promyelocytes are the myelocytes (Fig. 8 a - e). Generally these cells are somewhat smaller than their immediate precursors, with diameters ranging from 14 to 20 ...
Rat CCL2 is a member of the β (CC) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
Discrete bodies which may be designated cytochondria occupy the greater part of the cytoplasm of liver cells. A part, but not all, of these bodies have the characteristics of mitochondria. They consist of a rim which stains deeply and a central part which stains faintly or remains unstained.. In liver cells about portal spaces, in foci of regeneration, and in tumor cells the rims of the cytochondria stain with basic dyes and contain ribonucleic acid or related substances.. With increasing accumulation of basophile substances about cytochondria as the central veins are approached, these bodies aggregate to form clumps or orient themselves with palisade-like arrangment in two rows just within the margins of the liver cell columns.. With aggregation of the cytochondria that are surrounded by basophile material, other cytochondria in the same cells are devoid of this material and wholly acidophile.. With chromatolysis (disappearance of basophile substance) caused by dimethylaminoazobeiizene (butter ...
... or granules in the white blood cells to know if they are neutrophils basophils or lymphocytes. Optically Copper has stained the ...

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