Basophils: Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.Basophil Degranulation Test: An in vitro test used in the diagnosis of allergies including drug hypersensitivity. The allergen is added to the patient's white blood cells and the subsequent histamine release is measured.Histamine Release: The secretion of histamine from mast cell and basophil granules by exocytosis. This can be initiated by a number of factors, all of which involve binding of IgE, cross-linked by antigen, to the mast cell or basophil's Fc receptors. Once released, histamine binds to a number of different target cell receptors and exerts a wide variety of effects.Immunoglobulin E: An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Receptors, IgE: Specific molecular sites on the surface of B- and T-lymphocytes which combine with IgEs. Two subclasses exist: low affinity receptors (Fc epsilon RII) and high affinity receptors (Fc epsilon RI).Antigens, CD63: Ubiquitously-expressed tetraspanin proteins that are found in late ENDOSOMES and LYSOSOMES and have been implicated in intracellular transport of proteins.Hypersensitivity: Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.Mast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Antibodies, Anti-Idiotypic: Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.Anaphylaxis: An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.Eosinophils: Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.Allergens: Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).Leukotriene C4: The conjugation product of LEUKOTRIENE A4 and glutathione. It is the major arachidonic acid metabolite in macrophages and human mast cells as well as in antigen-sensitized lung tissue. It stimulates mucus secretion in the lung, and produces contractions of nonvascular and some VASCULAR SMOOTH MUSCLE. (From Dictionary of Prostaglandins and Related Compounds, 1990)Histamine: An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.Strongylida Infections: Infections with nematodes of the order STRONGYLIDA.Interleukin-4: A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.Nippostrongylus: A genus of intestinal nematode parasites belonging to the superfamily HELIGMOSOMATOIDEA, which commonly occurs in rats but has been experimentally transmitted to other rodents and rabbits. Infection is usually through the skin.Desensitization, Immunologic: Immunosuppression by the administration of increasing doses of antigen. Though the exact mechanism is not clear, the therapy results in an increase in serum levels of allergen-specific IMMUNOGLOBULIN G, suppression of specific IgE, and an increase in suppressor T-cell activity.Peanut Hypersensitivity: Allergic reaction to peanuts that is triggered by the immune system.Cell Degranulation: The process of losing secretory granules (SECRETORY VESICLES). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by EXOCYTOSIS.Filarioidea: A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.Anti-Allergic Agents: Agents that are used to treat allergic reactions. Most of these drugs act by preventing the release of inflammatory mediators or inhibiting the actions of released mediators on their target cells. (From AMA Drug Evaluations Annual, 1994, p475)Betula: A plant genus of the family BETULACEAE. The tree has smooth, resinous, varicolored or white bark, marked by horizontal pores (lenticels), which usually peels horizontally in thin sheets.Skin Tests: Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.Th2 Cells: Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.Urticaria: A vascular reaction of the skin characterized by erythema and wheal formation due to localized increase of vascular permeability. The causative mechanism may be allergy, infection, or stress.Hypersensitivity, Immediate: Hypersensitivity reactions which occur within minutes of exposure to challenging antigen due to the release of histamine which follows the antigen-antibody reaction and causes smooth muscle contraction and increased vascular permeability.Antigens, Plant: Substances found in PLANTS that have antigenic activity.Complement C5a: The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.Receptors, CCR3: CCR receptors with specificity for CHEMOKINE CCL11 and a variety of other CC CHEMOKINES. They are expressed at high levels in T-LYMPHOCYTES; EOSINOPHILS; BASOPHILS; and MAST CELLS.N-Formylmethionine Leucyl-Phenylalanine: A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.Interleukin-13: A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.Food Hypersensitivity: Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)CD-ROM: An optical disk storage system for computers on which data can be read or from which data can be retrieved but not entered or modified. A CD-ROM unit is almost identical to the compact disk playback device for home use.Loa: A genus of parasitic nematodes found throughout the rain-forest areas of the Sudan and the basin of the Congo. L. loa inhabits the subcutaneous tissues, which it traverses freely.Pituitary Gland, Anterior: The anterior glandular lobe of the pituitary gland, also known as the adenohypophysis. It secretes the ADENOHYPOPHYSEAL HORMONES that regulate vital functions such as GROWTH; METABOLISM; and REPRODUCTION.Learning: Relatively permanent change in behavior that is the result of past experience or practice. The concept includes the acquisition of knowledge.Laryngeal Edema: Abnormal accumulation of fluid in tissues of any part of the LARYNX, commonly associated with laryngeal injuries and allergic reactions.Lysophospholipase: An enzyme that catalyzes the hydrolysis of a single fatty acid ester bond in lysoglycerophosphatidates with the formation of glyceryl phosphatidates and a fatty acid. EC 3.1.1.5.Drug Hypersensitivity: Immunologically mediated adverse reactions to medicinal substances used legally or illegally.Hypersensitivity, Delayed: An increased reactivity to specific antigens mediated not by antibodies but by cells.Tolonium Chloride: A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.3,3'-DiaminobenzidineFishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.Leukotrienes: A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.Cytoplasmic Granules: Condensed areas of cellular material that may be bounded by a membrane.Horseshoe Crabs: An arthropod subclass (Xiphosura) comprising the North American (Limulus) and Asiatic (Tachypleus) genera of horseshoe crabs.

Evidence for the involvement of IgE-basophil system in acute serum sickness. (1/982)

The role of the basophils in acute serum sickness of rabbits was examined by monitoring daily the absolute number of basophils before, during and after the disease period. After antigen (bovine serum albumin, BSA) elimination, levels of serum IgE and in vitro basophil degranulation in the presence of BSA were determined. The results showed that the onset of glomerular lesions depends upon the simultaneous occurrence of circulating immune complexes greater than 19 S and of an in vivo basophil depletion--probably equivalent to degranulation--reaching 70% of the pre-disease number. Post-disease antigen-dependent in vitro degranulation of the basophils and levels of serum IgE anti BSA did not prove to be good indexes of basophil sensitization. Our data suggest that basophils are instrumental at early stages of the deposition of immune complexes, most probably through their sensitization by membrane-bound IgE antibodies.  (+info)

Detection of allergen-induced basophil activation by expression of CD63 antigen using a tricolour flow cytometric method. (2/982)

In the field of allergy diagnosis, most in vitro functional tests are focused on basophils. Nevertheless, the very small number of circulating basophils limits these experiments and their clinical benefit remains controversial. As flow cytometry is a valuable tool for identifying cell populations, even at low concentrations, we developed a tricolour flow cytometric method for the study of allergen-induced basophil activation. Identification of cells was based both on CD45 expression and on the presence of IgE on the cell surface, since basophils express high-affinity receptors for IgE (Fc epsilon RI). Cell activation upon allergen challenge was assessed by the expression of CD63 antigen on the plasma membrane. Basophil isolation and activation (with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine) were validated in 32 non-allergic patients. In 12 allergic patients, basophil stimulation by a relevant allergen was in most cases positive (10/12). Furthermore a concentration-dependent hook effect was observed. Of the allergic and non-allergic patients, none showed non-specific activation with an irrelevant allergen (specificity 100%). Overall, our preliminary results, even in a small population, suggest that this is a reliable and valuable method for the diagnosis of allergies complementing specific allergen IgE and skin test results. Obviously, additional clinical studies are needed to validate these first results.  (+info)

Extracellular signal-regulated kinases regulate leukotriene C4 generation, but not histamine release or IL-4 production from human basophils. (3/982)

Human basophils secrete histamine and leukotriene C4 (LTC4) in response to various stimuli, such as Ag and the bacterial product, FMLP. IgE-mediated stimulation also results in IL-4 secretion. However, the mechanisms of these three classes of secretion are unknown in human basophils. The activation of extracellular signal-regulated kinases (ERKs; ERK-1 and ERK-2) during IgE- and FMLP-mediated stimulation of human basophils was examined. Following FMLP stimulation, histamine release preceded phosphorylation of ERKs, whereas phosphorylation of cytosolic phospholipase A2 (cPLA2), and arachidonic acid (AA) and LTC4 release followed phosphorylation of ERKs. The phosphorylation of ERKs was transient, decreasing to baseline levels after 15 min. PD98059 (MEK inhibitor) inhibited the phosphorylation of ERKs and cPLA2 without inhibition of several other tyrosine phosphorylation events, including phosphorylation of p38 MAPK. PD98059 also inhibited LTC4 generation (IC50 = approximately 2 microM), but not histamine release. Stimulation with anti-IgE Ab resulted in the phosphorylation of ERKs, which was kinetically similar to both histamine and LTC4 release and decreased toward resting levels by 30 min. Similar to FMLP, PD98059 inhibited anti-IgE-mediated LTC4 release (IC50, approximately 2 microM), with only a modest effect on histamine release and IL-4 production at higher concentrations. Taken together, these results suggest that ERKs might selectively regulate the pathway leading to LTC4 generation by phosphorylating cPLA2, but not histamine release or IL-4 production, in human basophils.  (+info)

Down-regulation of human basophil IgE and FC epsilon RI alpha surface densities and mediator release by anti-IgE-infusions is reversible in vitro and in vivo. (4/982)

Previously, infusions of an anti-IgE mAb (rhumAb-E25) in subjects decreased serum IgE levels, basophil IgE and FcepsilonRIalpha surface density, and polyclonal anti-IgE and Ag-induced basophil histamine release responses. We hypothesized that these effects would be reversed in vivo by discontinuation of infusions and in vitro by exposing basophils to IgE. Subjects received rhumAb-E25 biweekly for 46 wk. Blood samples taken 0-52 wk after rhumAb-E25 were analyzed for serum IgE and basophil expression of IgE, FcepsilonRIalpha, and CD32. Basophil numbers were unaffected by infusions. Eight weeks after infusions, free IgE levels rose in vivo but did not reach baseline. Basophil IgE and FcepsilonRIalpha rose in parallel with free IgE while CD32 was stable. FcepsilonRI densities, measured by acid elution, returned to 80% of baseline, whereas histamine release responses returned to baseline. Basophils cultured with or without IgE or IgG were analyzed for expression of IgE, FcepsilonRIalpha, and CD32. By 7 days with IgE, expression of IgE and FcepsilonRIalpha rose significantly, whereas cultures without IgE declined. IgE culture did not effect CD32. IgG culture did not effect expression of any marker. The present results strongly suggest that free IgE levels regulate FcepsilonRIalpha expression on basophils.  (+info)

Adhesive explant culture of allergic nasal mucosa: effect of emedastine difumarate, an anti-allergic drug. (5/982)

Allergic reaction of the nose comprises of an immediate and a late reaction. To evaluate nasal allergic reactions, many experiments have been performed by investigators. In this study, we performed a new tissue culture technique (adhesive explant culture) to analyze the migration of cells into the culture medium from the cultured allergic nasal mucosa in response to an allergen. Basophilic cells (mast cells and basophils) and eosinophils, which were released into the culture medium after the allergen challenge, were evaluated by the analysis of histamine and eosinophil cationic protein (ECP) content in the culture medium. Histamine and basophilic cells in the culture medium were more abundant in the immediate phase (within 30 min) after challenge than in the late phase (from 30 min to 10 hr). On the other hand, ECP and eosinophils in the culture medium were more abundant in the late phase than in the immediate phase. The increase of histamine content in both phases were not inhibited by pre-treatment of emedastine difumarate (EME), an anti-allergic drug. However, the increase of ECP in the late phase was inhibited by pre-treatment with EME. Moreover, the number of EG2-positive cells was also decreased by pre-treatment with EME. These results suggest that EME might lower the activation of eosinophils in the late phase of the allergic reaction. The present study also indicates that this adhesive explant culture system is useful model for studying the cellular allergic responses to drugs ex vivo.  (+info)

The effect of processing on inflammatory markers in induced sputum. (6/982)

The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from healthy and atopic asthmatic subjects were treated with either DTE or phosphate-buffered saline (PBS) at 22 or 37 degrees C and compared for cell counts and concentrations of histamine, tryptase, eosinophil cationic protein (ECP), free interleukin (IL)-8, immunoglobulin (Ig)A, IL-8/IgA complexes and secretory component (SC). In addition, the influence of DTE on in vitro mediator release from blood eosinophils, basophils and bronchoalveolar lavage (BAL) mast cells was studied. Processing with DTE improved cytospin quality and increased the cell yield and measurable ECP, tryptase, IgA and SC, but reduced levels of histamine in PBS-treated samples and had no effect on IL-8. Cell counts or mediator levels were similar when sputum was processed at 22 or 37 degrees C, even though DTE induced blood basophils and BAL mast cells to release histamine at 37 degrees C. In spiking experiments, recovery of added ECP, tryptase, total IL-8 and histamine from sputum was similar in DTE- and PBS-processed sputum, but reduced for free IL-8 in PBS-treated samples. In conclusion, dithioerythritol improves cell and mediator recovery without causing cell activation when sputum processing is conducted at room temperature. The extent of recovery depends on the mediator studied.  (+info)

Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway: downregulation by sodium. (7/982)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 micrograms/ml). However, when extracellular Na+ was substituted iso-osmotically with N-methyl-d-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37 degrees for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM-CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mm, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+.  (+info)

Characterization of mast cell-committed progenitors present in human umbilical cord blood. (8/982)

Human mast cells are derived from CD34(+) hematopoietic cells present in cord blood, bone marrow, and peripheral blood. However, little is known about the properties of the CD34(+) cells. We demonstrated here that mast cell progenitors that have distinct phenotypes from other hematopoietic cell types are present in cord blood by culturing single, sorted CD34(+) cells in 96-well plates or unsorted cells in methylcellulose. The CD34(+) mast cell-committed progenitors often expressed CD38 and often lacked HLA-DR, whereas CD34(+) erythroid progenitors often expressed both CD38 and HLA-DR and CD34(+) granulocyte-macrophage progenitors often had CD33 and sometimes expressed CD38. We then cultured single cord blood-derived CD34(+)CD38(+) cells under conditions optimal for mast cells and three types of myeloid cells, ie, basophils, eosinophils, and macrophages. Of 1,200 CD34(+)CD38(+) cells, we were able to detect 13 pure mast cell colonies and 52 pure colonies consisting of either one of these three myeloid cell types. We found 17 colonies consisting of two of the three myeloid cell types, whereas only one colony consisted of mast cells and another cell type. These results indicate that human mast cells develop from progenitors that have unique phenotypes and that committed mast cell progenitors develop from multipotent hematopoietic cells through a pathway distinct from myeloid lineages including basophils, which have many similarities to mast cells.  (+info)

RESUMEN. The basophil activation test (BAT) has become a pervasive test for allergic response through the development of flow cytometry, discovery of activation markers such as CD63 and unique markers identifying basophil granulocytes.. Basophil activation test measures basophil response to allergen cross-linking IgE on between 150 and 2000 basophil granulocytes in ,0.1 ml fresh blood. Dichotomous activation is assessed as the fraction of reacting basophils.. In addition to clinical history, skin prick test, and specific IgE determination, BAT can be a part of the diagnostic evaluation of patients with food-, insect venom-, and drug allergy and chronic urticaria. It may be helpful in determining the clinically relevant allergen.. Basophil sensitivity may be used to monitor patients on allergen immunotherapy, anti-IgE treatment or in the natural resolution of allergy. Basophil activation test may use fewer resources and be more reproducible than challenge testing. As it is less stressful for the ...
Treatment of allergic disease by decreasing circulating IgE with anti-IgE Abs is currently under clinical study. Based on previous unrelated studies, it appeared likely that Fc(epsilon)RI expression on basophils and mast cells might also be regulated by levels of circulating IgE Ab. Therefore, the expression of IgE and Fc(epsilon)RI on human basophils was examined in 15 subjects receiving humanized anti-IgE mAb intravenously. Treatment with the anti-IgE mAb decreased free IgE levels to 1% of pretreatment levels and also resulted in a marked down-regulation of Fc(epsilon)RI on basophils. Median pretreatment densities of Fc(epsilon)RI were approximately 220,000 receptors per basophil and after 3 mo of treatment, the densities had decreased to a median of 8,300 receptors per basophil. Flow cytometric studies, conducted in parallel, showed similar results and also showed in a subset of 3 donors that receptors decreased with a t1/2 of approximately 3 days. The responsiveness of the cells to ...
The inhibitory myeloid immunoglobulin receptor CD300a (IRp60) has been shown to downregulate mast cell and eosinophil activities, thereby serving as a potential target for inhibiting allergic effector cell input in allergy. Our aims were to study the expression and functional properties of this receptor in purified human basophils, cells that crucially contribute to Th2-type immunity and allergy. Basophils homogeneously expressed CD300a as well as the inhibitory receptor CD200R on their cell surface, and these expressions increased after anti-IgE stimulation. IgE-mediated basophil degranulation was also significantly inhibited by crosslinking of either CD200R or CD300a (by 90% and 50%, respectively). Inhibitory SHIP-1 phosphorylations were also induced by CD200R and CD300a, although they were not noticeably increased by IgE-dependent activation. We conclude that both CD200R and CD300a play a role in reducing IgE-mediated basophil function and may crucially govern the known differential ...
Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled with histadine, proteolytic enzymes, leukotrienes, and proteoglycans. Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can be identified by expression of CD200R3 and FceRIa. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil because it is basophilic (readily takes up bases), and typically stains a relatively deep blue or purple. These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these cell types using standard staining techniques.) *Produced only in pregnancy by the developing embryo. B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology: CD-ROM and Guide. Oxford University Press. ISBN 978-0195151732. MedicalMnemonics.com: ...
Basophils arise and mature in bone marrow. When activated, basophils degranulate to release histamine, proteoglycans (e.g. heparin and chondroitin), and proteolytic enzymes (e.g. elastase and lysophospholipase). They also secrete lipid mediators like leukotrienes (LTD-4), and several cytokines. Histamine and proteoglycans are pre-stored in the cells granules while the other secreted substances are newly generated. Each of these substances contributes to inflammation. Recent evidence suggests that basophils are an important source of the cytokine, interleukin-4, perhaps more important than T cells. Interleukin-4 is considered one of the critical cytokines in the development of allergies and the production of IgE antibody by the immune system. There are other substances that can activate basophils to secrete which suggests that these cells have other roles in inflammation.[13] The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called ...
View details of top basophil count hospitals in Thane. Get guidance from medical experts to select best basophil count hospital in Thane
Basophil (好塩基球 Kōenkikyū) is a minor character in Cells at Work!. He is a type of white blood cell, specifically Basophil. He is capable of triggering the release of histamines when encountering an antigen. He also can allure eosinophils and neutrophils when they are needed. His role is not...
Basophil activation in unfractionated samples such as whole blood can be measured by changes in the expression of cell surface markers or even intracellular events (e.g. phosphorylation, oxidative burst, calcium flux) by flow cytometry. Separate markers (e.g. as CD123+ HLA-DR-) are used to specifically identify basophils in addition to those used for assessment of activation. A feature of this protocol is to stimulate the basophils both in the presence and absence of autologous plasma. This protocol is specifically for a mouse allergen SCIT study conducted at JHU. ...
A nice review about the early non-T-cell source of IL-4 from basophils that act as Th2-inducing antigen-presenting cells. Even though we have shown that during LN responses to alum-precipitated antigens IL-4 is dispensable for the early induction of Th2-features including B cell maturation and helper T cell differentiation more, it is important to keep in mind that the basophil-source of IL-4 may have implication during the clearance of helminth infections and the development/maintenance/ augmentation of allergic diseases by induction of IgE production, activation of mast cells and basophils, as well as eosinophilic inflammation. An important role for basophils is unquestionable for they have been shown (together with mast cells) to release large amounts of IL-4 and IL-13 after cross-linkage of their IgE receptor, Fce receptor I (FceRI), by complexes of antigen plus antigen-specific IgE. However, recent evidence has proposed a novel role in antigen presentation to CD4 T cells that could play ...
Basophil: Basophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by basic dyes and functionally by its role in mediating hypersensitivity reactions of the immune system. Basophils, along with eosinophils and neutrophils, constitute a group of white
The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine blue, on blood smears fixed with lead subacetate. Metachromasia was more identifiable through better preservation of the granules. The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil granules ...
A change in morphology and behavior of a basophil resulting from exposure to a cytokine, chemokine, soluble factor, or to (at least in mammals) an antigen which the basophil has specifically bound via IgE bound to Fc-epsilonRI receptors, leading to the in…
II.E.001. Normal basophil. Blood film. The field contains a basophil and lymphocyte. The dark blue basophilic granules characteristically obscure the nucleus. The granules are relatively large and easily resolved with the light microscope. In this case some cytoplasmic basophilic granules were lost in film preparation and the cell is partially degranulated as a result of the trauma of preparation. This finding is common in blood films.. ...
Define basophil: a basophilic substance or structure; especially : a white blood cell containing basophilic granules that is similar in function to a…
Fig 3.: Comparable to primary mouse basophils, the Hoxb8 variety is characterized by a relatively short half-life in culture, which was significantly prolonged after the addition of the cytokine interleukin-3 (IL-3). Cell viability (double negative annexin V/PI-population) was assessed by flow cytometry.. In summary, we have established a method to generate basophil-committed progenitor lines using Hoxb8. These progenitors can be induced in vitro to differentiate into mature basophils on a large scale, which, to the best of our knowledge, is a novel and unique attribute for a model of the kind. The here-described cell lines are genetically stable, can in principle be generated from any genetically modified strain of mice that survives up to embryonic stage E12, and can be easily handled in culture. Since large numbers of mice are required to isolate primary basophils, we would like to promote this cellular model as an alternative system to study specific aspects of basophil biology, and ...
Sato, S., 1975: Studies on supravital observation of basophils in bronchial asthma in immunological reaction part 1 changes of migration velocity of basophils by addition of anti immuno globulin
Remove the 2 mL Cryotube with 200 μL of the 20x basophil media, thaw and add 1.8 mL of warm 37°C ) RPMI medium. Close the stopper and vortex it for 5 sec. This is the 2X basophil media to be used for incubation with the whole blood and for preparation of the egg or peanut allergen dilutions (see below ...
Four subjects with clinical histories of milk-induced asthma were studied (three allergic to cows milk; one to soya milk). In each instance, skin prick tests, RAST (IgE and IgG4), the basophil histamine release, and serum precipitins, using appropri
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
Basophils have gained renewed interest as inducers and amplifiers of Th2 immunity through their potential to migrate to LNs, provide an early source of IL-4, and at the same time act as APCs in models of helminth infection and injection of the model protease papain, but have been largely ignored in the field of pulmonary mucosal immunity and development of allergic asthma to inhaled allergens (Sokol et al., 2008, 2009). Here, we found that basophils are recruited to the MLNs draining the site of i.n. administration of HDM allergen within 3 d of HDM injection. Several mechanisms could explain this increase. Like basophil expansion and recruitment to sites of helminth infection (Voehringer et al., 2006), we found that this process required adaptive immunity and CD4 T cells, as MHCII-deficient mice had severely reduced recruitment of basophils. The most likely explanation is T cell-derived production of IL-3 (Shen et al., 2008; Kim et al., 2010), which we found to be induced in CD4 T cells upon ...
H. Sasaki, D. Kurotaki, N. Osato, H. Sato, I. Sasaki, S. Koizumi, H. Wang, C. Kaneda, A. Nishiyama, T. Kaisho, H. Aburatani, H. C. Morse III, K. Ozato, T. Tamura. 2015. Transcription factor IRF8 plays a critical role in the development of murine basophils and mast cells. Blood 125:358-369 ...
This is a basophil (white blood cell or granular leukocyte) which is moderately advanced. Its diameter is approximately 10 micrometers. Again the basophilic granules tend to obscure the shape of the nucleus, however, there is a suggestion that the nucleus is, at this stage, bilobed. Wrights stain. x2150. - Stock Image P248/0294
Allergic subjects release significantly more histamine than normals, exposure to the relevant antigens (P23/TPT1: [§§] locus 13q12-q14) nonessential co-chaperones produces a cytokine (histamine-releasing factor) from a subpopulation of highly allergic donor basophils HRF/p23 can stimulate nonimmune epithelium late allergic reaction (LAR), causing them (basophils) to release histimines. Molecular chaperone machines: Hsp70/Hsp90 and most p53 mutations are associated with a compensatory mutant pair the cyclophilin Cyp-40 and several other polypeptides such as P23 between both partners is through Rx (Resistance to potato virus X) immune sensor stabilization in response to wild type GR glucocorticoid receptor pharmacological properties hormonal stimuli heterocomplexes GR with plant hsp90 are stabilized, principally CyP40, arrives late in receptor complex assembly. GR regulation requires its Hsp90 co-chaperone (PTGES3) function, but not its chaperone activity. A polyclonal rabbit antibody inhibited ...
Veja Foto de stock de Normal Human Peripheral Blood With Red Blood Cells And A Basophil And A Segmented Neutrophil Leukocyte Wright Stain X160 Lm X160. Encontre fotografias premium e de alta resolução na Getty Images.
Involvement of Hypoxia-Inducible Factor-1 in the Inflammatory Responses of Human LAD2 Mast Cells and Basophils. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
... tablets areused for oligospermia, as they improve the sperm count by increasing the LH- FSH producing basophil cells in the pituitary gland.

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Gibbs, Bernhard F and Rathling, A. and Hube, M. and Haas, Helmut (2007) Histamine in allergy, infl ammation, must cells and basophils: Substantial differences in the kinetics of histamine release from human basophils caused by varying strengths of IgE-dependent activators. Inflammation Research, 56 (Supplement 1). S05-S06 . ISSN 1023-3830 . (doi:https://doi.org/10.1007/s00011-006-0502-1 ) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided ...
Dot plots showing immunoreactivity pattern of blast cells. Basophilic cells account for 38% of the total nucleated cells. Blast cells showing positivity for (A)
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For a lovely, playful explanation of my sufferings, check out this video from ScienceGoddess, aka Joanne Manaster. (via BoingBoing)
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For your city. All we can offer a quality pet medications require strict testing before being excreted through your Accuhaler. The rare in Australia World Internet Project ReportARC Centre of Excellence in the treatment at the your Cialis cost reasonable. They have grown considerably over the counter and so cannot be replicated w. Quality Affordable Pet Meds delivers pet lovers to provide the student in their 30s and even they are shipped. Our drugs policies have failed. Viewing Tweets wont unblock BuyPainMeds2Day. cialis originale contrassegno. Rotations. the minimal 5 mg hire independent contractors for Why should you need to come online faster. The boot-up diagnostics and runtime diagnostics tests available on the synthetic. Read all the info and used his legs and arms. Basophils are a progressive clinical course for you. Buying Here. You honestly think that I was astonished. Student, Suzanne Borho October 26, 2014 Toxic Metals and Detoxification by Dr. Hoffman As a precaution, when the ...
Heparin SWOT Analysis And Forecast 2019 About Heparin Heparin is an anticoagulant or blood thinner produced by basophils and mast cells. Though it has no e
Blood. Fluid connective tissue Function: transport cells & dissolved matter Ground substance = plasma Formed elements = cells & cell fragments. 1-RBC 2-WBCs 4-Platelets. Blood. Erythrocytes (RBCs) - O2 & CO2 Leukocytes (WBCs) - immune functions Neutrophils Eosinophils, Basophils Slideshow 6579521 by britanni-stevens
...Histology MCQ Online With Answers...Which of the following is not a granulocyte? a. Lymphocytes b. Neutrophil c. PMN d. Eosinophils e. Basophils Answer: a
The concentration of RBC, thrombocytes, WBC and constituent WBC subtypes (monocytes, lymphocytes, neutrophils, basophils and eosinophils) were evaluated in blood drawn from controls and patients ...
undefined Mean Corpusc haemoglobin 28.7 (27.3 - 32.6) Platelet count 418 (140- 400) Neutrophil 4.1 (1.7 - 7.5) Eosinophil 0.1 (0.0 - 0.5) Basophil 0.1 (0.0 - 0.1) Monocyte 0....
When my childs blood was tested, we got the following results: Lymphocytes 51.0 (range 25 - 50), Monocytes 7.6 (range 1-6) NORMAL: WBC 7.7 (range 4.5-13.5) Neutrophils 38 (range 28 -66) Eosinophils 2.7 Basophils 0.7. What is the implication of these out of range values?
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By a confocal fluorescence microscope with an argon-ion laser (488 nm) and a He-Cd laser (325 nm) we have studied thapsigargin-induced calcium signals in individual rat basophilic leukaemia (RBL-2H3) cells. In the presence or absence of external calcium ions, thapsigargin-induced calcium signals were transferred to the nucleus as well as to the cytoplasm of RBL-2H3 cells. The calcium signals were generally much stronger in the nucleus than in the cytoplasm. However, some of the RBL-2H3 cells had apparently reduced nuclear calcium signals. They had a basophil-like bilobed (multilobed) nucleus, although most RBL-2H3 cells had a mast-cell-like monolobed nucleus. In the cells with a bilobed nucleus, IgE-receptor-mediated calcium signals were neither transferred to the nucleus nor to the cytoplasm. The results gave a new insight into the understanding of the mechanism of the nuclear calcium signals in RBL-2H3 cells. ...
Looking for basophil degranulation test? Find out information about basophil degranulation test. A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test
In the face of increasing prevalence of hypersensitivity reactions, introduction of effective, reliable and safe methods plays a crucial role in their diagnosing. Among the currently available laboratory (in vitro) methods is basophil activation test (BAT). It is a flow- cytometry based assay that allows to identificate in the blood sample basophils and additionally to asses the degree of cell activation after exposure to an antigen. The most common superficial identification markers are CD63 and CD203c, which increase in number after activation. Basophil actvation test can be applied to confirm diagnosis of allergy to Hymenoptera venoms, food, pollens and hypersensitivity to drugs. The aim of present paper is to present theoretical methods of this test as well as its pros and cons. We focus also on presentation of clinical case where BAT seemed to be a necessary addition to a routine diagnostic pathway. We present a case of identification of the culprit drug which caused an anaphylactic ...
Lipid rafts are plasma-membrane microdomains that are enriched in certain lipids (sphingolipids, glycosphingolipids and cholesterol), as well as in lipid-modified proteins. Rafts appear to exist in the liquid-ordered phase, which contributes to their partitioning from the surrounding liquid-disordered glycerophospholipid environment. DRM (detergent-resistant membrane) fractions isolated from cells are believed to represent coalesced lipid rafts. We have employed extraction using two different non-ionic detergents, Brij-96 and Triton X-100, to isolate detergent-resistant lipid rafts from rat basophilic leukaemia cell line RBL-2H3, and compared their properties with each other and with plasma-membrane vesicles. DRM fractions were isolated as sealed unilamellar vesicles of similar size (135-170 nm diameter), using either sucrose-density-gradient sedimentation or gel-filtration chromatography. Lipid rafts isolated using Brij-96 and Triton X-100 differed in density, protein content and the ...
Looking for online definition of basophil substance in the Medical Dictionary? basophil substance explanation free. What is basophil substance? Meaning of basophil substance medical term. What does basophil substance mean?
Looking for online definition of basophilia in the Medical Dictionary? basophilia explanation free. What is basophilia? Meaning of basophilia medical term. What does basophilia mean?
Resistance to infestation by larval Amblyomma americanum or Rhipicephalus sanguineus ticks was transferred to naive guinea pigs with peritoneal exudate cells (PEC) or serum from donors immunized by prior infestation with homologous tick larvae. In the A. americanum system, PEC transfer induced 87% tick rejection, which was similar to the level of resistance in actively sensitized hosts. In the R. sanguineus system, PEC conferred resistance (39% rejection) that was weaker than in actively sensitized hosts (57% rejection). In both systems, immune serum conferred significant but weaker resistance (20 to 29% rejection). In actively sensitized hosts, resistance to each tick species was specific, but there was considerable cross-reactive resistance. Basophils dominated the 24-hr challenge feeding sites of A. americanum ticks in actively sensitized hosts (69% of the infiltrate) and recipients of sensitized PEC (69%). Mononuclear cells were dominant (69% of the infiltrate) in the challenged tissues of ...
TY - JOUR. T1 - A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells. AU - Barth, Erin E.. AU - Hallworth, Richard J.. AU - Nichols, Michael G.. PY - 2005/5. Y1 - 2005/5. N2 - Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint will necessarily depend on the characteristics of the damage induced by the agent. By using multiple biological probes, it is possible to get a more complete description of the type of damage induced. Photodamage was induced in rat basophilic leukemia cells by either 254-nm UVC light exposure or rose bengal photosensitization. Damage was measured by three quantitative assays employing fluorescent probes: calcein, to measure nonspecific esterase activity, propidium iodide (PI), to measure loss of plasma membrane integrity, rhodamine 123 ...
TY - JOUR. T1 - Anaphylaxis to xylitol diagnosed by skin prick test and basophil activation test. AU - Okamoto, Kaoru. AU - Kagami, Michiko. AU - Kawai, Manabu. AU - Mori, Yuji. AU - Yamawaki, Kazuo. AU - Nakajima, Yoichi. AU - Kondo, Yasuto. AU - Tsuge, Ikuya. PY - 2019/1. Y1 - 2019/1. UR - http://www.scopus.com/inward/record.url?scp=85058961080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85058961080&partnerID=8YFLogxK. U2 - 10.1016/j.alit.2018.08.001. DO - 10.1016/j.alit.2018.08.001. M3 - Letter. C2 - 30190098. AN - SCOPUS:85058961080. VL - 68. SP - 130. EP - 131. JO - Allergology International. JF - Allergology International. SN - 1323-8930. IS - 1. ER - ...
The clavulanic acid (CLV) is nowadays frequently included in the treatment with Amoxicillin (AX). This component of the antibiotic therapy initially thought to have a low immunogenic capacity; however immediate allergic reactions to CLV have been reported in a 30% of patients allergic to AX-CLV. Basophil activation test (BAT) has shown promising results demonstrating specific recognition of CLV determinants. The aim of this study was to assess the value of BAT in the evaluation of immediate allergic reactions to CLV. ...
Basophils possess membrane bound IgE molecules, and immunological activation leads to a secretory process with cell degranulation and histamine release. Heterologous anti IgE, concanavaline A, and phytohaemagglutinin are potent non-cytotoxic releasing agents. They operate by a mechanism similar to that of immunological activation. Heavy water is not a histamine releasing inducer but it increases histamine release of the cells. We studied the histamine release reaction of leukaemic basophils in 10 patients and found a physiological response such as that previously reported with normal human basophils.. ...
Mercuric chloride (HgCl2) is an industrial agent known to cause autoimmune disorders and induce IgE synthesis, which plays a crucial role in the manifestation of allergic diseases. In rodents, the immunomodulatory effects of HgCl2 have been shown to involve the enhancement of mast cell-derived IL-4 secretion, which facilitates both Th2-lymphocyte development and IgE production. In humans, rapid allergen-dependent release of IL-4 and the related cytokine IL-13 from histamine-containing cells occurs primarily in basophils, along with other proinflammatory mediators such as histamine and LTC4. In this study, we therefore investigated the effects of HgCl2 on the release of the above basophil mediators, either due to the compound alone or in conjunction with IgE-dependent stimulation. HgCl2 (10(-9) to 10(-6) M) did not induce mediator secretion alone but significantly enhanced the release of histamine, LTC4, IL-4, and IL-13 caused by anti-IgE. Higher concentrations of HgCl2 (10(-5) to 10(-3) M) ...
Peachell P.T.; Macglashan D.W.Jr; Lichtenstein L.M.; Schleimer R.P., 1988: Regulation of human basophil and lung mast cell function by cyclic amp
Parekh, A. B.; Penner, R.: Activation of store-operated calcium influx at resting InsP(3) levels by sensitization of the InsP(3) receptor in rat basophilic leukaemia cells. Journal of Physiology-London 489 (2), pp. 377 - 382 (1995 ...
The Diamond Basophil Isolation Kit has been developed for the two-step isolation of basophilic granulocytes from human PBMCs. - Great Britain
The table below shows the top 200 pain related interactions that have been reported for Basophil Adenoma. They are ordered first by their pain relevance and then by number of times they were reported in Basophil Adenoma. Please click on the INT link to display more detailed information on each interaction. ...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
The conditions and cell sources for colony stimulating activity (CSA) production by nasal polyp epithelial scrapings were examined. Epithelial scrapings removed from patients were grown to confluence during 7 days as monolayers of epithelial cells in media supplemented with fetal calf serum (FCS) on collagen-coated microwell plates. Growth kinetics of nasal polyp epithelial cells (NPECs) were determined, and CSA in NPEC conditioned medium (CM) was assessed with density-gradient separated, nonadherent peripheral blood mononuclear cells in standard 14-day methylcellulose assays. Nasal polyp cultures in the presence of 5% or 15% FCS (vol/vol) demonstrated significantly more epithelial cell proliferation than cultures at 0% and 1% FCS. There were comparable metachromatic cell counts in polyp epithelial scrappings from allergic and nonallergic donors. Similarly, NPEC CM from allergic and nonallergic donors had equivalent CSA for basophil/mast cell (BMC) and eosinophil (EO) lineages, respectively. CSA ...
The ultrastructural study of the secretory cells type 1 and 2 confirmed the separate identities of two secretory cell types in the gut of female B. microplus. Secretory cell type 1 (s1) synthesized and secreted large, spherical, uniformly electrondense granules. Secretory cell type 2 (s2) synthesized smaller, irregularly shaped and more complex granules. Another cell type, the basophilic cell, was shown to be the reorganized basal remnant of secretory cell s2. A few of the basophilic cells retained remnant s2 granules within their cytoplasm. In these cells the reorganized cisternae of rough endoplasmic reticulum were arranged in whorls and parallel arrays. The cells synthesized granules with a different ultrastructure and position in the cell from the earlier granules. The new secretory material may be egg proteins which are released into the haemolymph, and transported to the ovary. Another secretory cell type with smaller spherical granules was seen in the gut caeca of only two female ticks ...
Current Research and Scholarly Interests The goals of Dr. Gallis laboratory are to understand the regulation of mast cell and basophil development and function, and to develop and use genetic approaches to elucidate the roles of these cells in health and disease. We study both the roles of mast cells, basophils, and IgE in normal physiology and host defense, e.g., in responses to parasites and in enhancing resistance to venoms, and also their roles in pathology, e.g., anaphylaxis, food allergy, and asthma, both in mice and humans. ...
Basophile Granulozyten sünd Zellen, de to de Bestanddeelen vunt Blood höört un to de Witten Bloodkörpers (Leukozyten) tellt warrt. De basophilen Granulozyten wiest tallrieke Granula op, de groff un nich regelmatig sünd und ünner annern Histamin un Heparin bargt. Int Differentialbloodbild maakt se blots en lütten Andeel vun 2 bit 6 % ut.[1] De basophilen Granulozyten stammt ut dat Knakenmark, se hebbt mit de eosinophilen Granulozyten en gemeesomen Vörlöper. Wassdomsfakters för de Basophilen sünd ünner annern Interleukin-3, Interleukin-5 un GM-CSF. Dat gifft Henwiesen dorop, dat sik de Basophilen un de Eosinophilen bit Riepen gegensietig kontrolleert. Ton Bispeel ünnerdrückt de transformeeren Wassdomsfakter TGF int Biwesen vun Interleukin-3 dat Differenzeren vun Eosinophilen un föddert dat vun de Basophilen. Se hebbt en Rezepter fört Immunglobulin E (IgE), wat to de Annahmföhrt, dat se en Rull speelt bi de Immunafwehr vunn Weert gegen Parasiten. Wenn jemehr Rezepters dör ...
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/ typeof=CreativeWork http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.deakin.edu.au/portal/Regulation-of-type-2-immunity-by-basophils-David/1dZ7SkuhJec/,Regulation of type 2 immunity by basophils, David Voehringer, (electronic resource),/a,,/span, - ,span property=offers typeOf=Offer,,span property=offeredBy typeof=Library ll:Library resource=http://link.library.deakin.edu.au/#Deakin%20University%20Library,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.deakin.edu.au/,Deakin University Library,/a,,/span,,/span,,/span,,/span,,/div ...
Some monocytes contain azurophilic granules much finer than those seen in lymphocytes. The nucleus is seldom round; usually it is deeply indented and lobulated (often bean-shaped). Its loose, delicate chromatin pattern is unique among the blood cells. Clumps of chromatin may be found scattered among larger, chromatin-poor areas. Nucleoli are rarely present. 55 4 · Individual Cells Fig. 13 a - d a b c d IV 56 Chapter IV · Blood and Bone Marrow Fig. In absolute terms, mitoses are more frequent than in the myeloblasts (Fig. 7 d - h). 39 4 · Individual Cells Fig. 7 a - d a b c d IV 40 Chapter IV · Blood and Bone Marrow Fig. 7 e - h IV e f g h 41 4 · Individual Cells Neutrophilic Myelocytes, Metamyelocytes, Band and Segmented Forms, also Eosinophilic and Basophilic Granulocytes and Tissue Basophils (Fig. 8 a - h) The offspring of the promyelocytes are the myelocytes (Fig. 8 a - e). Generally these cells are somewhat smaller than their immediate precursors, with diameters ranging from 14 to 20 ...
Rat CCL2 is a member of the β (CC) subfamily of chemokines that is a chemoattractant for monocytes and basophils but not eosinophils or neutrophils. Rat CCL2 is secreted as a 14 kDa glycoprotein monomer but noncovalent dimers probably occur. The first five amino acids of the mature protein are essential for activity; deletion of the N-terminal glutamine, which is pyrrolidone carboxylic acid modified, dramatically decreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis. The rat CCL2 propeptide shares 82% amino acid (a.a.) identity with mouse CCL2 over the 148 a.a. sequence and 57%, 52%, 52%, 52% and 52% a.a. identity with equine, human, porcine, canine and guinea pig CCL2, respectively, over the first 100 aa. Rat and mouse CCL2 have a 49 aa extension at the C-terminus as compared to human CCL2. Fibroblasts, tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation. CCL2 is best ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
Mast cells and basophils are generally seen as important effectors of allergic responses that produce and release more mediators than any other cell type. Recent evidence indicates that these cells can interact with virtually all other cell types and that they have many other functions directly interacting with other inflammatory cells. If you would like to discuss and find out more about mast cells and basophils: Please come and join us at the ...
Discrete bodies which may be designated cytochondria occupy the greater part of the cytoplasm of liver cells. A part, but not all, of these bodies have the characteristics of mitochondria. They consist of a rim which stains deeply and a central part which stains faintly or remains unstained.. In liver cells about portal spaces, in foci of regeneration, and in tumor cells the rims of the cytochondria stain with basic dyes and contain ribonucleic acid or related substances.. With increasing accumulation of basophile substances about cytochondria as the central veins are approached, these bodies aggregate to form clumps or orient themselves with palisade-like arrangment in two rows just within the margins of the liver cell columns.. With aggregation of the cytochondria that are surrounded by basophile material, other cytochondria in the same cells are devoid of this material and wholly acidophile.. With chromatolysis (disappearance of basophile substance) caused by dimethylaminoazobeiizene (butter ...
Histamine, b-imidazolethylamine is an intercellular mediator exerting potent effects on various target tissues. Histamine is stored in large amounts in metachromatic granules of mast cells and basophils, and it is one of the major mediators of immediate hypersensitivity. It is released actively from mast cells and basophils when challenged with allergens to which the allergic patient is sensitized or with non-specific histamine releasers such as IgE or pharmacological agents. Histamine is also an important mediator in the central nervous system and the gastric mucosa.. ...
Development of respiratory epithelium (RE) rudiment was studied in tissue culture after removal of mesenchyme (M) and respiratory tract (RT) in 13 days old embryos of A and C57BL mice. During long-term cultivation of intact RT, organotypic structures (branching bronchioles, alveolus-like cavities) developed. No epithelial organotypic structures developed in the presence of single M cells; explants were represented by layers of cubic epithelium. During long-term cultivation foci of atypical growth consisting of intensively proliferating basophilic cells with high nucleocytoplasmic ratio appeared in these explants. Regions of planocellular metaplasia with or without keratinization could be found in these foci. The frequency of atypical proliferates depended on the strain of donor mice and on the region of the explanted RT.. ...
The interleukin-3 receptor (also known as CD123 antigen ) is a molecule found on cells which helps transmit the signal of interleukin-3 , a soluble cytokine important in the immune system . The gene coding for the receptor is located in the pseudoautosomal region of the X and Y chromosomes . The receptor belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CD131) subunit . The gene for the alpha subunit is 40 kilobases long and has 12 exons . Cell types and function The receptor, found on pluripotent progenitor cells, induces tyrosine phosphorylation within the cell and promotes proliferation and differentiation within the hematopoietic cell lines. It can be found on basophils and pDCs as well as some cDCs among peripheral blood mononuclear cells. CD123 is expressed across acute myeloid leukemia (AML) subtypes, including leukemic stem cells . Possible drug target An experimental antibody-drug conjugate SGN-CD123A targets
転写因子IRF8による好塩基球とマスト細胞の分化制御 [in Japanese] Regulation of basophil and mast cell development by the transcription factor IRF8 [in Japanese] ...
The Granulocytes and the agranulocytes are the two categories in which the WBC are divided. Following are the actions of WBC:. NEUTROPHILS: They are also knows as the Polymorphonuclear leukocytes or the PMN. It is most common type of WBC. In total WBC it accounts for about 65%. They act as the first line defense or the primary defenders against the infection caused by the bacteria of fungus.. The neutrophils are the first responders in destruction of foreign bodies, i.e., bacteria and fungi. Checking the PMN count does the diagnosis of the infection. These are the first WBC to increase in number during the infection. They are commonly seen during the inflammatory process and in the pus secretions. There are four signs of inflammation; Rubor (Redness), Dolor (Pain), Calor (Temperature), Tumor (Edema). The fifth sign added is the loss of function, called as the Functio-lasa.. BASOPHILS: The main function of the Basophils is to release the histamine, which is a chemical response to an infection. ...
A cell type of the immune system. Granulocytes are filled with granules of toxic chemicals that enable them to digest microorganisms. Basophils, neutrophils, and eosinophils are examples of granulocytes. See also Basophil; Eosinophil; Neutrophil ...
They are your bodys army. They are present to fight off invaders, such as viruses, bacteria, and allergens. When your body is attacked by something harmful, white blood cells increase and fight off the invader. A high white blood cell count can indicate infection. Lymphocytes are a type of white blood cell. They are further broken down into T cells and B cells. B-cells make antibodies to attack their enemies. T cells attack cells that have already been harmed by viruses or cancer. Neutrophils are the most common type of white blood cell. Their job is to destroy harmful bacteria. Monocytes can be broken down into macrophages (which "eat" the invader), or dendritic cells. Eosinophils and basophils are also part of the white blood count, but in a much smaller number. They also help to fight off parasites. Interestingly, when their numbers get too high, they can contribute to allergies in the body. ...
The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein ...
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Offers nationwide testing services specializing in Neuro-Endo-Immune assessments that can be utilized to quantitatively assess and address imbalances involved as the main contributors to common clinical conditions. Specialty tests include urinary neurotransmitters, salivary hormones, mitochondrial superoxide, Natural Killer (NK) cells, cytokines, lymphocyte subset analysis, MELISA® metal allergy, basophil activation, and iSpot Lyme T-cell ELISPOT technology testing services.. ...
There are seven types of white blood cells, two of which are primarily involved in the inflammation process. Eosinophils control allergic responses, while basophils release histamine during an inflammatory response ...
The natural killer (NK), T lymphocyte, B lymphocyte antigen (NTB-A) is a 60-kDa transmembrane glycoprotein of the CD2 family expressed on all human resting and activated NK, T and B cells. This antigen is not expressed on monocytes, neutrophils, and basophils but is expressed on eosinophils.
WBC is an acronym for white blood cells. These cells, also called leukocytes, help prevent infection. There are five major types of leukocytes: eosinophils, basophils, lymphocytes, neutrophils and monocytes. A blood test measures the number of these cells.
in a patient (male 17 years old been healthy all throught out his life) who happened to get a lab test done for no reason at all and all through all showed normal RBC counts and hematocrit of 4.5 million for RBC and 42 for hematocrit. and showed to have a WBC count of 99,000 on june 7 2008 with a neutrophil count of 42 %and lymphocyte count of 11% and basophil of 2 ...
BioAssay record AID 828777 submitted by ChEMBL: Open TG-GATES: Regimen: Daily Repeat; Time: 4 day; Dose: Middle; Route: intravenous injection | Dataset: Hematology; Assay: BASOLE (Basophils/Leukocytes); Study_ID: 617/9.
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BioAssay record AID 942514 submitted by ChEMBL: DrugMatrix: Regimen: Daily Repeat; Time: 7 day; Dose: M1; Vehicle: 100 % saline; Route: oral gavage | Dataset: Hematology; Assay: BASOLE (Basophils/Leukocytes); Study_ID: S010924.
n: heparin, Lipo-Hepin, Liquaemin} a polysaccharide produced in basophils (especially in the lung and liver) and that inhibits the activity of thrombin in coagulation of the blood; it (trade names Lipo-Hepin and Liquaemin) is used as an anticoagulant in the treatment of thrombosis and in heart surgery ...
Immediate selective hypersensitivity reactions to pyrazolones are thought to be mediated by specific-IgE antibodies. The diagnosis of selective reactions to pyrazolones is mainly based on the clinical history, skin testing and/or drug provocation test (DPT). Skin testing has low sensitivity and has potential risk of eliciting an anaphylactic response as well as DPT. Therefore the use of in vitro tests could be a safe and straightforward approach, although till now no well-validated test is available. Basophil activation test (BAT) has been proposed as a new in vitro diagnostic tool for different drug reactions although its sensibility is not optimal. The aim of this study was to analyze the potential usefulness of dipyrone metabolites to improve BAT sensitivity. ...
Most histamine in the body is generated in granules in mast cells and in white blood cells called basophils andeosinophils. Mast cells are especially numerous at sites of potential injury -- the nose, mouth, and feet, internal body surfaces, and blood vessels. Non-mast cell histamine is found in several tissues, including the brain, where it functions as a neurotransmitter. Another important site of histamine storage and release is the enterochromaffin-like (ECL) cell of the stomach.. The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic. These cells, if sensitized byIgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen. Certain amines and alkaloids, including such drugs as morphine, and curare alkaloids, can displace histamine in granules and cause its release. Antibiotics likepolymyxin are also found to stimulate histamine release.. Histamine release occurs when allergens bind to mast-cell-bound IgE ...
Hemocytes of Mytilus edulis were examined cytologically and cytochemically. On the basis of structure, staining reactions, and phagocytic behavior, they were divided into two main groups: basophilic hemocytes and eosinophilic granular hemocytes (granulocytes). The basophilic cells were further divided into small lymphocytes and larger phagocytic macrophages reactive for lysosomal hydrolases. Mitosis was observed in granulocytes and in small lymphoid cells, believed to be the stem cells for the basophilic cell line. A few cells appeared to be intermediate between lymphocytes and small granulocytes. Macrophages were the main cell type involved in the clearance of injected carbon particles. However, granulocytes did show some phagocytic activity. Brown cells displaying apparent amoebocytic behavior were found to contain Fe3+ and Pb2+ in cytoplasmic inclusions, some of which were also reactive for β-glucuronidase and glucosaminidase. These cells appear to have a separate origin from the ...
This study shows that histamine is liberated from human basophils by betaxolol and metipranolol and, to a significantly lesser extent, by timolol. The fact that we detected LDH in the supernatant indicated that the β blocker concentrations we used were cytotoxic to leucocytes. This cytotoxic effect may also explain the histamine liberation because we do not consider that high concentrations of β blockers are less toxic to basophils than to other leucocytes. Also, in previous studies other authors have used LDH levels to determine the cytotoxic effects on basophils in similar test conditions.14 Betaxolol and timolol formulations are commercially available in 0.1%, 0.25%, and 0.5% concentrations and metipranolol formulations are commercially available in 0.1%, 0.3%, and 0.6% which equals approximately 2.5 × 10-3 to 2 × 10-2 M. The cytotoxicity found in these experiments therefore occurs at concentrations that are used in commercial formulations. However, it should be noted that, upon ...
U.S., June 15 -- ClinicalTrials.gov registry received information related to the study (NCT03182491) titled Mechanisms of Anaphylaxis on June 7. Brief Summary: The purpose of this study is to explore different mechanisms for anaphylaxis and find novel biomarkers for this hypersensitivity syndrome. The study participants are patients with anaphylaxis, patients with mild allergic reactions, and patients with febrile transfusion reactions. We will also include a group of healthy controls. Study Start Date: Study Type: Observational [Patient Registry] Condition: Anaphylaxis Allergy Transfusion Reaction Febrile Transfusion Reaction Intervention: Diagnostic Test: Biomarkers (platelet activating factor [PAF], anaphylatoxins) and basophil activation test (BAT) Analysis of biomarkers and basophil activation test Recruitment Status: Recruiting Sponsor: Haukeland University Hospital Information provided by (Responsible Party): Haukeland University Hospital Published by HT Digital Content Services with ...
Clone FR3-16A11 recognizes CD203c, a glycosylated type II transmembrane molecule that belongs to the family of ecto-nucleotide pyrophosphatase/ phosphodiesterase (E-NPP3) enzymes. Among hematopoietic cells, expression of CD203c is restricted to basophils as well as to mast cells and their precursors, and has been described as specific for this lineage. Protein and/or mRNA expression of CD203c has also been found in solid tissues such as uterus or prostate. Basophils and mast cells are key producers of mediators that drive the onset of inflammatory responses, e.g., in allergy. Allergen challenge leads to a rapid up-regulation of activation markers such as CD203c or CD63. Due to its restricted expression pattern, CD203c is discussed as a specific marker to monitor the allergen-induced activation of basophils, e.g., in flow cytometric basophil activation tests of the peripheral blood. - Great Britain
Allergic diseases represent a significant burden in industrialized countries, but why and how the immune system responds to allergens remain largely unknown. Because many clinically significant allergens have proteolytic activity, and many helminths express proteases that are necessary for their life cycles, host mechanisms likely have evolved to detect the proteolytic activity of helminth proteases, which may be incidentally activated by protease allergens. A cysteine protease, papain, is a prototypic protease allergen that can directly activate basophils and mast cells, leading to the production of cytokines, including IL-4, characteristic of the type 2 immune response. The mechanism of papains immunogenic activity remains unknown. Here we have characterized the cellular response activated by papain in basophils. We find that papain-induced IL-4 production requires calcium flux and activation of PI3K and nuclear factor of activated T cells. Interestingly, papain-induced IL-4 production was dependent
Food allergy is defined as an adverse immunological response to food. Some of the tests discussed in this chapter not only aid in the diagnosis of food allergies, but also are useful in monitoring the natural history of patients' food allergies over time, from diagnosis to oral tolerance. Once food allergy has been identified as the likely cause of symptoms, confirmation of the diagnosis and identification of the implicated food(s) can begin. There are a number of tools that aid in the diagnosis of food allergy, some of which are more commonly used, and they vary in their ability to provide an accurate diagnosis. Available studies include in vivo tests such as skin prick and intradermal testing, oral food challenges (OFCs), elimination diets, and patch testing and in vitro tests such as quantification of food-specific IgE and basophil histamine release (BHR). Tests that have not proven helpful in food allergy diagnosis include quantification of food-specific IgG, total serum IgE levels, ...
U.S. Environmental Protection Agency (EPA) provide evaluations and is despostory of cataloged analysis. They also rank problems like, like indoor air pollution. commonly called sick building syndrome. They state that it is costing businesses $60 billion annually. Ill will get into the data later but I want to discuss, how do we protect human health and quality of life? By reducing chemical exposure and improve indoor air quality?. What Im finding out its not just the enviroment, its a humans immune system. Im not trying to be clever but create a debate that simply states that IAQ is a moving target. Molds that are everywhere, I have to refere to an expert, Serum IgE "Specific to Indoor Molds, Measured by Basophil Histamine Release, is Associated with Building-related Symptoms in Damp Buildings" FROM: Inflamm Res 2001; 50 (4) Apr: 227-31 Lander F, Meyer HW, Norn S National Work Environment Authority, Copenhagen, Denmark. [email protected] dk OBJECTIVE: To study the relationship between ...
Looking for Charcot-Leyden crystal? Find out information about Charcot-Leyden crystal. a solid body bounded by natural plane faces that are the external expression of a regular internal arrangement of constituent atoms, molecules, or ions. Explanation of Charcot-Leyden crystal
We have studied the cytotoxic activity of rat basophilic leukemia (RBL) cells transfected with cDNAs for the cytotoxic T lymphocyte (CTL) granule components, cytolysin (perforin), granzyme A, and granzyme B. With red cell targets, cytolysin expression conferred potent hemolytic activity, which was not influenced by coexpression of granzymes. With tumor targets, RBL cells expressing cytolysin alone were weakly cytotoxic, but both cytolytic and nucleolytic activity were enhanced by coexpression of granzyme B. RBL cells expressing all three CTL granule components showed still higher cytotoxic activities, with apoptotic target death. Analysis of the cytotoxic activity of individual transfectant clones showed that cytolytic and nucleolytic activity correlated with granzyme expression but was independent of cytolysin expression within the range examined. A synergism between granzymes A and B was apparent when the triple transfectant was compared with RBL cells expressing cytolysin and one granzyme. ...
Spleen tyrosine kinase (Syk) is a key regulatory factor in the IgE-mediated allergic signal transduction pathway in mast cells and basophils. Syk is phosphorylated on a number of tyrosines following the binding of IgE/allergen complexes to FcɛRI receptors leading to initiation of inflammatory signaling via downstream enzymes and scaffolding proteins. We examined the kinases responsible for the phosphorylation of key Syk tyrosines in rat RBL-2H3 basophilic cells and bone marrow-derived mast cells (BMMCs). The phosphorylation of Syk tyrosine 346 was completely blocked by the novel Src family kinase inhibitor BIRA766, suggesting this tyrosine is a pure substrate for Src family kinases ...
Hypersensitivity reactions to betalactams (BLs) are classified as immediate or nonimmediate. The former usually appear within 1 h of drug-intake and are mediated by specific IgE-antibodies. Nonimmediate reactions are those occurring more than 1 h after drug-intake, and they can be T-cell mediated. The diagnostic evaluation of allergic reactions to BLs has changed over the last 5 years, for several reasons. Major and minor determinants are no longer commercially available for skin testing in many countries. In immediate allergic reactions, the sensitivity of skin testing and immunoassays is decreasing and new in vitro methods, such as the basophil activation test, are gaining importance for diagnosis. For nonimmediate reactions, skin testing appears to be less sensitive than previous results, although more studies need to be carried out in this direction. Nevertheless, the drug provocation test is still necessary for diagnosis. ...
B. Eberlein Basophil activation test in the diagnosis of insect venom allergies Clinical & Experimental Allergy 39. Version of Record online: 21 OCT 2009 , DOI: 10.1111/j.1365-2222.2009.03375.x. Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf. Required = Required Field. ...
In 3rd or greater complete remission after a marrow or extramedullary relapse. (d) Patients with acute undifferentiated, biphenotypic, or bilineal leukemia, which is in 1st or greater complete remission (CR) or partial remission (PR). Cr will be defined as an M1 marrow (,5% blasts), no evidence of extramedullary disease, and an absolute neutrophil count ≥ 1.0 x 109/L. CR without platelet recovery (CRp) will be considered complete remissions.) PR will be defined as an M2 marrow (5-19% blasts), no evidence of extramedullary disease, and an absolute neutrophil count ≥ 1.0 x 109/L.).. (e) Chronic myelogenous leukemia (CML). (i) Chronic phase with resistance to tyrosine kinase inhibitors. (ii) accelerated phase (development of cytogenetic abnormality in addition to t(9:22), blood blast percentage ≥10, blood basophil percentage ≥20, platelet count ,100,000 X 109/L) (iii) blast crisis. (iv) 2nd or greater chronic phase.. (f) Acute Lymphoblastic Lymphoma in 2nd or greater complete remission. ...
Main article: Basophil. Basophils are chiefly responsible for allergic and antigen response by releasing the chemical histamine ... Basophil. 0.4%. 12-15. *Release histamine for inflammatory responses. Bi-lobed or tri-lobed. Large blue. A few hours to a few ... Basophils can also release chemical signals that attract eosinophils and neutrophils to an infection site.[9] ... basophils, lymphocytes, and monocytes.[2] These types are distinguished by their physical and functional characteristics. ...
Basophils have receptors that can bind to IgE, IgG, complement, and histamine. The cytoplasm of basophils contains a varied ... Histamine causes dilation and increased permeability of capillaries close to the basophil. Injured basophils and other ... Basophils are one of the least abundant cells in bone marrow and blood (occurring at less than two percent of all cells). Like ... When an infection occurs, mature basophils will be released from the bone marrow and travel to the site of infection.[22] When ...
Basophils: plump women who carry a basket of "histamine grenades" and throw them to attack bacteria. ...
A naive (or inexperienced) B cell is one which belongs to a clone which has never encountered the epitope to which it is specific. In contrast, a memory B cell is one which derives from an activated naive or memory B cell. The activation of a naive or a memory B cell is followed by a manifold proliferation of that particular B cell, most of the progeny of which terminally differentiate into plasma B cells;[note 8] the rest survive as memory B cells. So, when the naive cells belonging to a particular clone encounter their specific antigen to give rise to the plasma cells, and also leave a few memory cells, this is known as the primary immune response. In the course of proliferation of this clone, the B cell receptor genes can undergo frequent (one in every two cell divisions)[8] mutations in the genes coding for paratopes of antibodies. These frequent mutations are termed somatic hypermutation. Each such mutation alters the epitope-binding ability of the paratope slightly, creating new clones of ...
Basophils, or basophil granulocytes, are rare granulocytes. If you collected 1000 white blood cells, only 1-3 of them would be ... Basophils carry histamine and heparin. They appear at the sites of ectoparasite infection, or allergies. We don't know exactly ... As with mast cells and basophils, they part causes of allergy and asthma. Eosinophils are round cells with a lobed nucleus and ... basophils. Their nucleus is hidden by granules which turn dark blue in color when stained. ...
Hess, Charles E. Mature Basophil. University of Virginia Health System. [2009-04-10].. ...
Basophils. Langerhans cells. Monocytes Degranulation. Phagocytosis FcεRII (CD23) IgE Low (Kd , 10−7 M) B cells. Eosinophils. ... Prussin C, Metcalfe DD (February 2006). "5. IgE, mast cells, basophils, and eosinophils". The Journal of Allergy and Clinical ... mast cells and basophils.[13][14] As a result of its cellular distribution, this receptor plays a major role in controlling ... basophils, human platelets, and mast cells - that contribute to the protective functions of the immune system. Its name is ...
Basophils: May be increased in bone marrow related conditions such as leukemia or lymphoma.[9] ...
... basophils, and Th2 cells. DP2 activation also stimulates eosinophils and basophils to release the many pro-allergic elements of ... basophils, and Th2 cells at sites of nascent inflammation in animal models. PGD2, acting through DP2, stimulates the in vitro ... and basophils via seven-transmembrane receptor CRTH2". The Journal of Experimental Medicine. 193 (2): 255-61. doi:10.1084/jem. ... DP2 messenger RNA is also expressed by human basophils, eosinophils, a subpopulation of Cytotoxic T cells (i.e. CD8+ T cells), ...
that gives rise to basophils. Some sources use the term "CFU-Bas". Tsuda T, Wong D, Dolovich J, Bienenstock J, Marshall J, ...
... basophils, monocytes, and eosinophils; or 2) lymphoid precursor cells which differentiate into T lymphocytes, B lymphocytes, or ...
Basophil Gonads Growth of reproductive system Luteinizing hormone Lutropin LH, ICSH Glycoprotein Gonadotrophs Basophil Gonads ... Basophil Thyroid gland Secretion of thyroid hormones Follicle-stimulating hormone - FSH Glycoprotein Gonadotrophs ... Basophil Adrenal gland Secretion of glucocorticoid, mineralocorticoid and androgens Thyroid-stimulating hormone Thyrotropin TSH ... Nota bene: The term "Basophil" and "Acidophil" is used by some books, whereas others prefer to not use these terms. This is due ...
Basophils have receptors that can bind to IgE, IgG, complement, and histamine. The cytoplasm of basophils contains a varied ... Histamine causes dilation and increased permeability of capillaries close to the basophil. Injured basophils and other ... this polypeptide is produced by basophils. Basophils are one of the least abundant cells in bone marrow and blood (occurring at ... When basophils are injured, they will release histamine, which contributes to the inflammatory response that helps fight ...
Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. ...
It can be found in basophils. It induces prolonged, slow contraction of smooth muscle and has a major bronchoconstrictor role ...
Blom T, Hellman L (1993). "Characterization of a tryptase mRNA expressed in the human basophil cell line KU812". Scand. J. ... Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. ... and Fc epsilon RI gamma mRNAs in human mast cells and basophils by competitive reverse transcription-polymerase chain reaction ...
LTD-4 is released by basophils. Other leukotrienes that function in a similar manner are leukotrienes C4 and E4. ...
... basophils, and eosinophils; and other processes). In a new development in the field of antibody-based therapeutics, the Fc ...
Basophils leave the bone marrow already mature, whereas the mast cell circulates in an immature form, only maturing once in a ... The mast cell is very similar in both appearance and function to the basophil, another type of white blood cell. Although mast ... The Fc region of immunoglobulin E (IgE) becomes bound to mast cells and basophils and when IgE's paratopes bind to an antigen, ... Mast cells are very similar to basophil granulocytes (a class of white blood cells) in blood. Both are granulated cells that ...
Prussin C, Metcalfe DD (Feb 2006). "5. IgE, mast cells, basophils, and eosinophils". The Journal of Allergy and Clinical ... mast cells and basophils. As a result of its cellular distribution, this receptor plays a major role in controlling allergic ... and basophils. Fc receptors are found on a number of cells in the immune system including phagocytes like macrophages and ... basophils, human platelets, and mast cells - that contribute to the protective functions of the immune system. Its name is ...
Pharmacological modulation of Mast cells and Basophils. 778: 44-55. doi:10.1016/j.ejphar.2015.04.045. PMC 4636979 . PMID ...
It is constitutively expressed on mast cells and basophils and is inducible in eosinophils. FcεRI is found on epidermal ... Crosslinking of the FcεRI via IgE-antigen complexes leads to degranulation of mast cells or basophils and release of ... Prussin C, Metcalfe D (2006). "5. IgE, mast cells, basophils, and eosinophils". J Allergy Clin Immunol. 117 (2 Suppl Mini- ... Under laboratory conditions, degranulation of isolated basophils can also be induced with antibodies to the FcεRIα, which ...
Basophils and eosinophils are related to neutrophils. They secrete chemical mediators that are involved in defending against ... Type I hypersensitivity is mediated by IgE, which triggers degranulation of mast cells and basophils when cross-linked by ... basophils, and natural killer cells. These cells identify and eliminate pathogens, either by attacking larger pathogens through ...
Characteristically, basophil (e.g. CD11b, CD123) and monocyte markers (CD14, CD15) are absent. The cells usually express CD2 ...
Basophils as APC in Th2 Response in Allergic Inflammation and Parasite Infection. Current Opinion in Immunology, 2010 Dec; 22(6 ... Basophils and Allergic Inflammation. Journal of Allergy and Clinical Immunology, 2013 Oct;132(4):789-801; doi: 10.1016/j.jaci. ...
A white blood cell with granules that stain with basic dyes and are water-soluble Explanation of basophil degranulation test ... Looking for basophil degranulation test? Find out information about basophil degranulation test. ... redirected from basophil degranulation test). Also found in: Dictionary, Thesaurus, Medical, Legal, Financial. basophil. [′bās· ... Basophil degranulation test , Article about basophil degranulation test by The Free Dictionary https://encyclopedia2. ...
"Basophil". medcell.med.yale.edu.. *^ Voehringer D (December 2009). "The role of basophils in helminth infection". Trends in ... The word basophil uses combining forms of baso- + -phil, yielding "base-loving". ... Basophils are a type of white blood cell. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ... The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test ...
In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these ... In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these ... Authoritative and easy to use, Basophils and Mast Cells: Methods and Protocols will provide the necessary tools for future ... Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease ...
Synonyms: Pituitary gland Basophilic adenoma, mucoid cell adenoma, basophilic pituitary gland adenoma, Adenoma, Basophil, ... pituitary gland basophilic adenoma, pituitary gland basophil adenoma, basophilic adenoma, pituitary basophilic adenoma ...
Basophil tests can help doctors diagnose a range of health problems, including autoimmune conditions and allergies. Learn more ... Basophils are a type of white blood cell that plays a vital role in keeping the immune system functioning. ... about the function of basophils and what high and low levels mean in this article. ... If basophil levels are low, this may be a sign of an allergic reaction or another condition. High basophil levels may indicate ...
basophil* A type of white blood cell (leucocyte [1]) that has a lobed nucleus surrounded by granular cytoplasm (see granulocyte ... 2]). Basophils are produced continually by stem cells in the red bone marrow [3] and move about in an amoeboid fashion. ... 2. an increase in the number of basophils in the blood.. Cite this article Pick a style below, and copy the text for your ... basophil (bay-sŏ-fil) 1. n. a variety of white blood cell (see polymorph) distinguished by the presence in its cytoplasm of ...
Alkistis N. Mitropoulou, Holly Bowen, Tihomir S. Dodev, Anna M. Davies, Heather J. Bax, Rebecca L. Beavil, Andrew J. Beavil, Hannah J. Gould, Louisa K. James, and Brian J. Sutton ...
The term cutaneous basophil hypersensitivity (CBH) initially referred to a systemic but evanescent pattern of delayed-onset ... Dvorak, H. F., Colvin, R. B., and Churchill, W. H., 1975, Specificity of basophils and lymphocytes in cutaneous basophil ... Dissociation between cutaneous basophil hypersensitivity and basophil sensitization. J. Immunol. 129: 1262-1266.PubMedGoogle ... Rejection of ticks from guinea pigs by anti-hapten-antibody mediated degranulation of basophils at cutaneous basophil ...
Basophils, along with eosinophils and neutrophils, constitute a group of white ... Basophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be stained by basic dyes ... blood: Basophils. Basophils are the least numerous of the granulocytes, and their large granules almost completely obscure the ... More About Basophil. 6 references found in Britannica articles. role in. immunity. * In immune system: Granulocytes ...
The occurrence of basophils in teleost blood was confirmed as very rare and was not caused by failure to preserve the basophil ... The occurrence of basophils in peripheral blood of 15 freshwater teleosts was examined using the metachromatic stain, toluidine ...
Basophils are a specific type of white blood cell. These cells are readily stained with basic dyes (this is where the name ... of this basophil. Basophils make up only a small portion of the number of white blood cells but are important parts of the ... Basophils are a specific type of white blood cell. These cells are readily stained with basic dyes (this is where the name ...
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
... S. I. Yalkut,1 E. V. Gulling,2 T. Yu. Gots,2 S. A ... Premyslov, "Low molecular weight thymic factor inhibits histamine release from basophils," Mediators of Inflammation, vol. 2, ...
Basopenia (a low basophil count) is difficult to demonstrate as the normal basophil count is so low; it has been reported in ... Basophils are a type of white blood cells. Basophils are the least common of the granulocytes, representing about 0.5 to 1% of ... This suggests that basophils may play a role in the immune response to these viruses. Basophils of mice and humans have ... The degranulation of basophils can be investigated in vitro by using flow cytometry and the so-called basophil-activation-test ...
The immune system uses basophils to release histamine, which increases blood flow to the part of the body thats under attack ... A diagram of white blood cells, including basophils. Basophils are part of the bodys innate immunity. In other words, these ... The primary purpose of basophils is to release histamine in response to an antigen, or an allergen such as pet dander. The ... Basophils are part of the bodys innate immune system that will be present at birth. ...
An anterior pituitary basophil is a type of cell in the anterior pituitary which manufactures hormones. It is called a basophil ... These basophils are further classified by the hormones they produce. (It is usually not possible to distinguish between these ... B-FLAT for Basophils: FSH, LH, ACTH, TSH Histology image:14002loa from Vaughan, Deborah (2002). A Learning System in Histology ...
... including Mast cells and Basophils. Basophils contain many granules inside the cell, which are filled with a variety of active ... Pilot study on basophil activation induced by contrast medium. Fundam Clin Pharmacol 2011;25:267 - 276 Janeway CA, Jr.; et al ... Several minutes at room temperature gives the marker time to bind to the CD63 proteins on the cell membrane of the basophil. A ... Because they outnumber by far the leucocytes they need to be removed to do a FACS analysis of the Basophils. Innate immune ...
Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils ... Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled ... can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can ...
basophils 1.6% - RBC count 4.3 - haematocrit 39.4% - MCHb 31.6 Pg - haemoglobin 13. - MCV 91.6 Cu.Microns - platelets count 295 ... Communities>Chronic Fatigue Syndrome>I Always fall asleep, dizzy, high basophils in blood ...
Umbilical cord blood basophils, For immunohistochemistry (IHC),biological,biology supply,biology supplies,biology product ... UCB Basophil Chip-IHC from 3H Biomedical AB, ... UCB Basophil Chip-IHC from 3H Biomedical AB. Company. 3H ... Basophil Chip-IHC from 3H Biomedical AB. 2. UCB Neutrophil Chip-IHC from 3H Biomedical AB. 3. MoDC Cell Chip-IHC from 3H ... Umbilical cord blood basophils, For immunohistochemistry (IHC). Info. 3H Biomedical AB. Dag Hammarskjlds vg 34, House Generalen ...
BioLegend offers a wide array of Basophils Antibodies Products. BioLegend develops and manufactures world-class, cutting-edge ...
BASOPHILS. Basophils form up to 1% of the total leucocytes. They are identified by the presence of coarse basophilic granules ... Increase in basophil leucocytes (counts above 0.1 X 10^9/liters) is uncommon, but this is seen in myeloproliferative disorders ... Disorders like myxedema, smallpox, chicken pox and ulcerative colitis also may be associated with basophil leucocytosis. ... Clinical Significance Of Blood Formation In Basophils, Monocytes, Thrombocytes And Lymphocytes. Updated on January 18, 2014 ...
Effects of basophils and basophil supernatants on B cell CD23 and CD40 expression. Basophils are known to produce mediators ... Therefore, the impact of basophils, basophil-supernatants, and basophil-mediators on the upregulation of CD23 and CD40 on B ... and basophil mediators on B cell CD23 and CD40 expression was investigated. Our data demonstrate that basophils and basophil- ... Basophils enhance the numbers of CD23+ and CD40+ B cells. Enhancement of (A) CD23+ or (B) CD40+ B cell numbers by basophils ...
Basophil (close-up). Basophil (close-up). Basophils are a specific type of white blood cell. These cells are readily stained ... of this basophil. Basophils make up only a small portion of the number of white blood cells but are important parts of the ...
A month later I had a repeat, platelets back to normal but slightly raised basophil. She said I might of had an infection or ... Raised basophil Hi, bit of a long one. Last year I had really bad health anxiety. I was convinced My glands in My neck were ... Re: Raised basophil Thank you for your reply. I did the worse thing and googled what it could mean when I got the results last ... Re: Raised basophil Those are inflammation markers. You had some swollen glands and then a cold sore... Cause = Effect. Told ...
  • Doctors believe that the role of basophils in the body is reactionary, meaning that their number will generally only rise or fall due to an invader or underlying chronic issue. (medicalnewstoday.com)
  • The results provide the basis for further investigations on the modulating role of basophils in diseases such as allergy and autoimmunity. (jimmunol.org)
  • This book aims to provide an up-to-date account of mast cell and basophil biology, together with an insight into the functional relevance of these cells in some tissue types. (textbookx.com)
  • When antibodies of the immunoglobulin E (IgE) class bind to specialized receptor molecules on basophils, the cells release their stores of inflammatory chemicals, including histamine, serotonin , and leukotrienes. (britannica.com)
  • Basophils also express several surface markers that stimulate surrounding cells, for example CD62L ( 10 ), CD40L ( 11 ), OX40-L ( 12 ), and receptor activator of NF-κB ligand ( 13 , 14 ). (jimmunol.org)
  • However, OIT did not significantly suppress this response in basophils stimulated by the distinct fMLP receptor pathway. (nih.gov)
  • Human basophils also express minute amounts of the GPI-anchored low-affinity IgG receptor FcγRIIIB ( 22 ), whose biological properties are unclear. (jimmunol.org)
  • The one characteristic that unites all types of mast cells and basophils appears to be the possession of the high affinity membrane receptor for immunoglobulin E, through which the cells may be stimulated. (textbookx.com)
  • Several investigators have reported that levels of SYK may correlate with basophil responsiveness and that SYK is degraded as a consequence of receptor-induced anergy. (openwetware.org)
  • Additionally, PLZF-deficient basophils had reduced expression of the IL-18 receptor, which impacted migration to lungs. (jimmunol.org)
  • Prostaglandin (PG) D 2 acts through both the DP 1 receptor, which is coupled to adenylyl cyclase, and the DP 2 receptor (chemoattractant receptor-homologous molecule expressed on Th2 cells), which is present on eosinophils, basophils, and Th2 cells and results in cell activation and migration. (aspetjournals.org)
  • This compound elicited DP 2 receptor-mediated CD11b expression in human basophils and eosinophils and induced actin polymerization and migration in eosinophils with a potency about the same as that of PGD 2 . (aspetjournals.org)
  • Depletion of basophils using an antibody directed to the high-affinity receptor for IgE (FcεRI) led to strongly reduced Th2 immunity in these models, whereas adoptive transfer of only basophils could induce Th2 polarization in naive antigen-specific T cells, without the necessity of DCs. (rupress.org)
  • To assess the requirement for basophil infiltration to acquired tick resistance, mice expressing the human diphtheria toxin receptor under the control of the mast cell protease 8 (Mcpt8) promoter were generated. (pubmedcentralcanada.ca)
  • Under laboratory conditions, degranulation of isolated basophils can also be induced with antibodies to the FcεRIα, which crosslink the receptor. (wikipedia.org)
  • Basophils are part of the body's innate immunity . (wisegeek.com)
  • Basophils are part of the body's innate immune system that will be present at birth. (wisegeek.com)
  • In this study, we show that the transcription factor PLZF, which is known for its essential role in the function and development of several innate lymphocyte subsets, is also important for the myeloid-derived basophil lineage. (jimmunol.org)
  • Recent studies in mice suggest that basophils may also regulate the behavior of T cells and mediate the magnitude of the secondary immune response. (wikipedia.org)
  • FcγR therefore function as a regulatory module, made of two subunits with antagonistic properties, that prevents IgG-induced and controls IgE-induced basophil activation in both mice and humans. (jimmunol.org)
  • Contrasting with the in vivo roles of human basophils, which can be primarily documented with correlations, the in vivo roles of mouse basophils can be investigated experimentally in wild-type (wt) mice, the basophils of which were depleted by appropriate Abs, or in genetically-modified mice that lack basophils. (jimmunol.org)
  • It was, however, not abrogated in basophil-deficient mice ( 5 ). (jimmunol.org)
  • This study aimed to investigate the role of basophil activation in the development of SLE based on studies in patients with SLE and spontaneous lupus-prone MRL- lpr/lpr mice. (frontiersin.org)
  • Importantly, basophil-depleted MRL- lpr/lpr mice exhibited an extended life span, improved renal function, and lower serum levels of autoantibodies and IL-17, while basophil-adoptive-transferred mice exhibited the opposite results. (frontiersin.org)
  • PLZF-deficient mice have reduced numbers of basophil progenitors and mature basophils. (jimmunol.org)
  • Although some liver basophils from naive mice express GFP, Nb infection enhanced GFP expression and increased the number of tissue basophils. (rupress.org)
  • Similar basophil GFP expression was found in infected Stat6 −/− mice. (rupress.org)
  • Rag2 −/− mice reconstituted with CD4 T cells allowed significant basophil accumulation, indicating that CD4 T cells can direct both tissue migration of basophils and enhanced IL-4 production. (rupress.org)
  • See commentary " Mice that "conditionally" lack basophils, AT LAST " on page 2648. (pubmedcentralcanada.ca)
  • Diphtheria toxin administration to these mice selectively ablated basophils. (pubmedcentralcanada.ca)
  • Wolf-Jurgensen, 1965, 1967), the description of CBH focused renewed attention on the occurrence of basophils in delayed-onset immunologic reactions, particularly in the guinea pig. (springer.com)
  • Cryostat sections of giant papillae were immunostained with basophil-specific antibody BB-1, and with anti-CD4, anti-CD8, anti-CD20, anti-major basic protein (MBP), anti-IgE and anti-FcɛRI-β antibodies. (bmj.com)
  • Down-regulation of Fc(epsilon)RI expression on human basophils during in vivo treatment of atopic patients with anti-IgE antibody. (jimmunol.org)
  • These data provide the first clear evidence, to our knowledge, that basophils play an essential and nonredundant role in antibody-mediated acquired immunity against ticks, which may suggest new strategies for controlling tick-borne diseases. (pubmedcentralcanada.ca)
  • Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. (rupress.org)
  • Inflammatory DCs were necessary and sufficient for induction of Th2 immunity and features of asthma, whereas basophils were not required. (rupress.org)
  • Moreover, murine basophils support memory responses and the survival of in vivo differentiated PC ( 22 , 23 ). (jimmunol.org)
  • The term cutaneous basophil hypersensitivity (CBH) initially referred to a systemic but evanescent pattern of delayed-onset reactivity in guinea pigs sensitized to protein antigens administered without mycobacterial adjuvants (Richerson et al . (springer.com)
  • Basopenia (or basocytopenia) is a form of agranulocytosis associated with a deficiency of basophils. (wikipedia.org)
  • Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. (wikipedia.org)