Generation and characterization of SCARs by cloning and sequencing of RAPD products: a strategy for species-specific marker development in bamboo. (1/45)

BACKGROUND AND AIMS: The aim of this study was to develop species-specific molecular markers for Bambusa balcooa and B. tulda to allow for their proper identification, in order to avoid unintentional adulteration that affects the quality and quantity of paper pulp production. METHODS: Two putative, species-specific RAPD markers, Bb836 for B. balcooa and Bt609 for B. tulda were generated using a PCR-based RAPD technique. Species-specificity of these two markers was confirmed through Southern hybridization in which RAPD gels were blotted and hybridized with radiolabelled cloned RAPD markers. Southern hybridization analyses were also performed to validate homology of the co-migrating Bb836 and Bt609 marker bands amplified from 16 different populations of B. balcooa and B. tulda, respectively. Sequence-characterized amplified region (SCAR) markers were developed from Bb836 and Bt609 sequences, using 20-mer oligonucleotide primers designed from both the flanking ends of the respective RAPD primers. KEY RESULTS: As anticipated, Bb836 hybridized with an amplified band from B. balcooa and Bt609 hybridized only with an amplified product from B. tulda; the two markers did not hybridize with the amplified products of any of the other 14 bamboo species studied. The two pairs of SCAR primers amplified the target sequences only in the respective species. The species-specific SCAR fragments were named as 'Balco836' for B. balcooa and 'Tuldo609' for B. tulda. The species-specific 'Balco836' was amplified from the genomic DNA of 80 individuals of 16 populations of B. balcooa studied. Similarly, the presence of 'Tuldo609' was noted in all the 80 individuals representing 16 populations of B. tulda assessed. These SCAR fragments contained no obvious repetitive sequence beyond the primers. CONCLUSION: These two molecular markers are potentially useful for regulatory agencies to establish sovereign rights of the germplasms of B. balcooa and B. tulda. In addition, this is the first report of species-specific SCAR marker development in bamboo.  (+info)

Botulism from home-canned bamboo shoots--Nan Province, Thailand, March 2006. (2/45)

On March 15, 2006, multiple persons with symptoms of nausea, vomiting, abdominal pain, and dyspnea visited the emergency department at Baan Luang district hospital in Nan province, Thailand; one person required mechanical ventilation. A team from the Bureau of Epidemiology, Department of Disease Control, Thailand Ministry of Public Health (MOPH) initiated an investigation, in collaboration with the Surveillance and Rapid Response Team from Baan Luang district. This report summarizes the investigation conducted during March 15-26, which determined that the outbreak was caused by foodborne botulism from home-canned bamboo shoots and affected 163 rural villagers who shared a common meal. The last case was identified March 21; no further cases of foodborne botulism have been identified in the region.  (+info)

Morphological and molecular characterization of Bambusa tulda with a note on flowering. (3/45)

BACKGROUND AND AIMS: Flowering incidence in Bambusa tulda has a high socio-economic impact. The aim of the present study was to describe the species for morphological characters (vegetative and reproductive) as well as molecular markers in order to enable species identification at various stages of the life cycle. METHODS: Thirty-two key morphological characters (15 culm and 17 culm-sheath) were studied along with detailed inflorescence and floral characters. Incidence of sporadic flowering was recorded. Genomic DNA was isolated from leaves collected from 17 eco-geographical locations and RAPD profiles were generated. KEY RESULTS: The description of culm, culm-sheath, inflorescence and floral morphology are in agreement with the prior taxonomic description by Gamble in 1896, but in this communication a more detailed description and illustrations are presented. No seed set was recorded following sporadic flowering, probably due to prezygoting isolating mechanisms (herkogamy or protandry). All 17 populations surveyed generated identical RAPD profiles. CONCLUSIONS: Sporadic flowering may occur in B. tulda, but may not necessarily be followed by gregarious flowering, and does not result in seed production.  (+info)

Lig-8, a bioactive lignophenol derivative from bamboo lignin, protects against neuronal damage in vitro and in vivo. (4/45)

Lig-8, a lignophenol derivative from bamboo lignin, potently suppresses oxidative stress-induced apoptosis. Here, we first examined in vitro whether lig-8 protects against neuronal damage induced by oxygen-glucose deprivation (OGD) followed by reoxygenation, tunicamycin [endoplasmic reticulum (ER)-stress inducer], or PSI (proteasome inhibitor). In pheochromocytoma (PC12) cell cultures, lig-8 (1 to 30 microM) concentration-dependently inhibited OGD- and tunicamycin (2 microg/ml)-induced cell deaths (significant at >/=3 microM and >/=1 microM, respectively). In human neuroblastoma (SH-SY5Y) cell culture, the PSI-induced apoptotic cell death and fusion protein accumulation (revealing reduced proteasome activity) was inhibited by lig-8 (30 microM). On the other hand, lig-8 at 30 microM alone did not affect any proteasome activity under resting conditions. In vivo, lig-8 (0.1 nmol/eye) reduced intravitreal N-methyl-D-aspartate (NMDA, 20 nmol)-induced retinal damage (decreases in retinal ganglion cells and inner plexiform layer thickness). Hence, lig-8 protects, partly by inhibiting excessive ER-stress, against neuronal damage in vitro and in vivo.  (+info)

Volatile organic compounds with characteristic odor in bamboo vinegar. (5/45)

Bamboo vinegar solutions had pHs of 2.5 to 2.8, and the amounts of organic constituents were estimated to be 2.3 to 4.6% (w/w). Volatile organic compounds (28 components) were detected by GC-MS, and among of these, 11 compounds were common to three samples of bamboo vinegar. Perhaps acetic acid, 3-methyl-1,2-cyclohexadione, guaiacol, p-cresol, and syringol contributed to the characteristic odors (sour, smoky, and medicinal note) in bamboo vinegar.  (+info)

Laboratory study on the effect of deltamethrin WG and WP formulations against Anopheles maculatus Theobald (Diptera:Culicidae) on rough and smooth surfaces of bamboo wall. (6/45)

Adults of Anopheles maculatus were tested for their residual activity to wettable powders (WP) and water dispersible granule (WG) formulations of deltamethrin. The residual effectiveness and lifespan of deltamethrin WG and WP were also assessed against the mosquitoes using rough and smooth surfaces of bamboo. Tests were conducted once a month up to 14 months after spraying using WHO standard method for the bioassay of insecticidal deposits on wall surfaces. Mortality data revealed that both deltamethrin WG and WP were effective against An. maculatus up to 14 months post-spraying. Efficacy and residual activity of deltamethrin WG at 25mg/m2 had proved to be the longest on both rough and smooth surfaces of bamboo.  (+info)

Phytotoxic effects of (+/-)-catechin in vitro, in soil, and in the field. (7/45)

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Isolation and identification of a new hypocrellin A-producing strain Shiraia sp. SUPER-H168. (8/45)

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