Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. The type species is Autographa californica nucleopolyhedrovirus.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
The genetic complement of a BACTERIA as represented in its DNA.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Time period from 1501 through 1600 of the common era.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
An extensive order of highly specialized insects including bees, wasps, and ants.
A venomous New World spider with an hourglass-shaped red mark on the abdomen.
Insects of the suborder Heterocera of the order LEPIDOPTERA.
A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by ovicylindrical occlusion bodies. The type species is Cydia pomonella granulovirus.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The outer protein protective shell of a virus, which protects the viral nucleic acid.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Exclusive legal rights or privileges applied to inventions, plants, etc.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The misinterpretation of a real external, sensory experience.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
International collective of humanitarian organizations led by volunteers and guided by its Congressional Charter and the Fundamental Principles of the International Red Cross Movement, to provide relief to victims of disaster and help people prevent, prepare for, and respond to emergencies.
The systematic arrangement of entities in any field into categories classes based on common characteristics such as properties, morphology, subject matter, etc.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
A genus of the family RETROVIRIDAE consisting of non-oncogenic retroviruses that produce multi-organ diseases characterized by long incubation periods and persistent infection. Lentiviruses are unique in that they contain open reading frames (ORFs) between the pol and env genes and in the 3' env region. Five serogroups are recognized, reflecting the mammalian hosts with which they are associated. HIV-1 is the type species.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.
A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).
A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.
A protein serine-threonine kinase that catalyzes the PHOSPHORYLATION of I KAPPA B PROTEINS. This enzyme also activates the transcription factor NF-KAPPA B and is composed of alpha and beta catalytic subunits, which are protein kinases and gamma, a regulatory subunit.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
An oligomer formed from the repetitive linking of the C-terminal glycine of one UBIQUITIN molecule via an isopeptide bond to a lysine residue on a second ubiquitin molecule. It is structurally distinct from UBIQUITIN C, which is a single protein containing a tandemly arrayed ubiquitin peptide sequence.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.
A component of NF-kappa B transcription factor. It is proteolytically processed from NF-kappa B p100 precursor protein and is important for maturation of B-LYMPHOCYTES and adaptive HUMORAL IMMUNITY.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A plant genus in the family ROSACEAE, order Rosales, subclass Rosidae. It is best known as a source of the edible fruit (apple) and is cultivated in temperate climates worldwide.
A saturated 12-carbon fatty alcohol obtained from coconut oil fatty acids. It has a floral odor and is used in detergents, lubricating oils, and pharmaceuticals. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The use of wings or wing-like appendages to remain aloft and move through the air.
The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.

Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. (1/2544)

To understand the initiation of the transcription of protein-coding genes, we have dissected the role of the basal transcription/DNA repair factor TFIIH. Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides, we were able to analyze the role of XPB, XPD, and cdk7 subunits in the transcription reaction. Designing mutated recombinant subunits, we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase, which is dispensable, stimulates transcription and allows the CAK complex to be anchored to TFIIH. In addition, we also show that cdk7 may phosphorylate the carboxy-terminal domain (CTD) of RNA pol II in the absence of promoter opening.  (+info)

A plant 126-kDa phosphatidylinositol 4-kinase with a novel repeat structure. Cloning and functional expression in baculovirus-infected insect cells. (2/2544)

Phosphatidylinositol metabolism plays a central role in signaling pathways in animals and is also believed to be of importance in signal transduction in higher plants. We report here the molecular cloning of a cDNA encoding a previously unidentified 126-kDa phosphatidylinositol (PI) 4-kinase (AtPI4Kbeta) from the higher plant Arabidopsis thaliana. The novel protein possesses the conserved domains present in animal and yeast PI 4-kinases, namely a lipid kinase unique domain and a catalytic domain. An additional domain, approximately 300 amino acids long, containing a high percentage (46%) of charged amino acids is specific to this plant enzyme. Recombinant AtPI4Kbeta expressed in baculovirus-infected insect (Spodoptera frugiperda) cells phosphorylated phosphatidylinositol exclusively at the D4 position of the inositol ring. Recombinant protein was maximally activated by 0.6% Triton X-100 but was inhibited by adenosine with an IC50 of approximately 200 microM. Wortmannin at a concentration of 10 microM inhibited AtPI4Kbeta activity by approximately 90%. AtPI4Kbeta transcript levels were similar in all tissues analyzed. Light or treatment with hormones or salts did not change AtPI4Kbeta transcript levels to a great extent, indicating constitutive expression of the AtPI4Kbeta gene.  (+info)

Baculovirus expression and biochemical characterization of the human microsomal triglyceride transfer protein. (3/2544)

The microsomal triglyceride transfer protein (MTP) complexed to protein disulphide isomerase (PDI) is obligatory for the assembly of chylomicrons and very-low-density lipoproteins. The determination of the atomic structure of the MTP-PDI heterodimer has important implications for the treatment of those forms of hyperlipidaemia associated with the overproduction of very-low-density lipoproteins, which predispose to premature coronary heart disease. To perform structural studies of the human MTP-PDI complex it was necessary to produce milligram quantities of pure protein. We chose the baculovirus expression system for this purpose. Insects cells were co-infected with recombinant viruses encoding FLAG-tagged MTP and His-tagged PDI; the resulting heterodimer was purified by affinity chromatography. From 5 litres of insect cells, 4-6 mg of more than 95% pure recombinant protein was obtained. CD and attenuated total reflection Fourier-transform infrared spectroscopy indicate that the purified protein has around 34% alpha-helical and 33% beta-structure content. The recombinant protein had a comparable triglyceride transfer activity to that of bovine MTP-PDI. The production of polyclonal antibodies raised against the MTP and PDI subunits of the purified protein is described. The present study demonstrates the feasibility of expressing two proteins at high levels in insect cells and describes a transferable methodology for the purification of the resulting protein complex.  (+info)

A lipid modified ubiquitin is packaged into particles of several enveloped viruses. (4/2544)

An anti-ubiquitin cross-reactive protein which migrates more slowly (6.5 kDa) by SDS-PAGE than ubiquitin was identified in African swine fever virus particles. This protein was extracted into the detergent phase in Triton X-114 phase separations, showing that it is hydrophobic, and was radiolabelled with both [3H]palmitic acid and [32P]orthophosphate. This indicates that the protein has a similar structure to the membrane associated phosphatidyl ubiquitin described in baculovirus particles. A similar molecule was found in vaccinia virus and herpes simplex virus particles, suggesting that it may be a component of uninfected cell membranes, which is incorporated into membrane layers in virions during morphogenesis.  (+info)

Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus. (5/2544)

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.  (+info)

Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. (6/2544)

Although great progress has been made in the characterization of the biochemical and biological features of hepatitis C virus (HCV) gene expression, the elucidation of the HCV life cycle and the evaluation of novel antiviral strategies have been hindered by the lack of a suitable cell culture system. In this context, the development of an efficient HCV cDNA delivery method would contribute to the understanding of HCV replication. To assess the functionality of baculovirus mediated gene delivery for HCV expression, we have constructed recombinant baculoviruses encoding HCV cDNA under the control of the cytomegalovirus promoter. Transduction of the human hepatoma cell line Huh-7 with Bac-HCV vectors was efficient and HCV cDNA expression was enhanced by treatment of the infected cells with dexamethasone. HCV structural and nonstructural polypeptides were processed correctly and were found to localize in the cytoplasm in a pattern characteristic of the endoplasmic reticulum. The expression of the HCV proteins was detected for 49 days after infection. Thus, these results indicate that the recombinant Bac-HCV vectors are a useful tool for the delivery of HCV cDNA and can facilitate the analysis of structural and functional properties of the HCV proteins. In addition, the Bac-HCV vectors can provide important information on the evaluation of novel anti-HCV antiviral strategies.  (+info)

Reovirus virion-like particles obtained by recoating infectious subvirion particles with baculovirus-expressed sigma3 protein: an approach for analyzing sigma3 functions during virus entry. (7/2544)

Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a partial way for the major outer-capsid protein sigma3, we obtained in vitro assembly of large numbers of virion-like particles by binding baculovirus-expressed sigma3 protein to infectious subvirion particles (ISVPs) that lack sigma3. A level of sigma3 binding approaching 100% of that in native virions was routinely achieved. The sigma3 coat in these recoated ISVPs (rcISVPs) appeared very similar to that in virions by electron microscopy and three-dimensional image reconstruction. rcISVPs retained full infectivity in murine L cells, allowing their use to study sigma3 functions in virus entry. Upon infection, rcISVPs behaved identically to virions in showing an extended lag phase prior to exponential growth and in being inhibited from entering cells by either the weak base NH4Cl or the cysteine proteinase inhibitor E-64. rcISVPs also mimicked virions in being incapable of in vitro activation to mediate lysis of erythrocytes and transcription of the viral mRNAs. Last, rcISVPs behaved like virions in showing minor loss of infectivity at 52 degrees C. Since rcISVPs contain virion-like levels of sigma3 but contain outer-capsid protein mu1/mu1C mostly cleaved at the delta-phi junction as in ISVPs, the fact that rcISVPs behaved like virions (and not ISVPs) in all of the assays that we performed suggests that sigma3, and not the delta-phi cleavage of mu1/mu1C, determines the observed differences in behavior between virions and ISVPs. To demonstrate the applicability of rcISVPs for genetic studies of protein functions in reovirus entry (an approach that we call recoating genetics), we used chimeric sigma3 proteins to localize the primary determinants of a strain-dependent difference in sigma3 cleavage rate to a carboxy-terminal region of the ISVP-bound protein.  (+info)

Production and characterization of a soluble, active form of Tva, the subgroup A avian sarcoma and leukosis virus receptor. (8/2544)

The receptor for the subgroup A avian sarcoma and leukosis viruses [ASLV(A)] is the cellular glycoprotein Tva. A soluble form of Tva, sTva, was produced and purified with a baculovirus expression system. Using this system, 7 to 10 mg of purified sTva per liter of cultured Sf9 cells was obtained. Characterization of the carbohydrate modification of sTva revealed that the three N glycosylation sites in sTva were differentially utilized; however, the O glycosylation common to Tva produced in mammalian and avian cells was not observed. Purified sTva demonstrates significant biological activity, specifically blocking infection of avian cells by ASLV(A) with a 90% inhibitory concentration of approximately 25 pM. A quantitative enzyme-linked immunosorbent assay, developed to assess the binding of sTva to ASLV envelope glycoprotein, demonstrates that sTva has a high affinity for EnvA, with an apparent dissociation constant of approximately 0.3 nM. Once they are bound, a very stable complex is formed between EnvA and sTva, with an estimated complex half-life of 6 h. The soluble receptor protein described here represents a valuable tool for analysis of the receptor-envelope glycoprotein interaction and for structural analysis of Tva.  (+info)

GlycoBac LLC provides insect cell lines, baculovirus vectors, and plasmids for the production of recombinant proteins in the baculovirus insect cell system
Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression Vector System (BEVS). We discuss the different strategies used to generate recombinant baculovirus vectors and show how advanced techniques for virus titer determination can accelerate the production of recombinant proteins. The stable transfection of insect cells is an alternative to BEVS which has recently been augmented with recombinase-mediated cassette exchange for site-specific gene integration. We consider the advantages and limitations of these techniques for the production of recombinant proteins in insect cells and compare them to other expression platforms.
Baculovirus gene transfer into Mammalian cells, known from scientific research articles as BacMam, is the use of baculovirus to deliver genes to mammalian cells. Baculoviruses are insect cell viruses that can be modified to express proteins in mammalian cells. The unmodified baculovirus is able to enter mammalian cells, however its genes are not expressed unless a mammalian recognizable promoter is incorporated upstream of a gene of interest. Both unmodified baculovirus and baculovirus modified with a mammalian promoter (BacMam) are unable to replicate in humans and are thus non infectious. Invented by Dr. Frederick M. Boyce, BacMam is a baculovirus-mediated gene transfer technique that has gained widespread use because of advantages when compared to other transfection methods, (for reviews see, Kost, T.A. et al,). In addition, BacMam has been found to have inherent flexibility over stable cell lines, which has contributed to its adoption as a standard gene transfer technique. The BacMam gene ...
Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, diptera, and decapoda serve as natural hosts. There are currently 49 species in this family, divided among 4 genera. Baculoviruses are known to infect invertebrates, with over 600 host species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture they are not known to be capable of replication in mammalian or other vertebrate animal cells. Starting in the 1940s they were used and studied widely as biopesticides in crop fields. Baculoviruses contain circular double-stranded genome ranging from 80 to 180 kbp. The earliest records of baculoviruses can be found in the literature from as early as the sixteenth century in reports of wilting disease infecting silk-producing larva. Starting in the 1940s they were used and studied widely ...
Baculovirus virions have a complex structure which consists of an envelope and a rod-shaped nucleocapsid. The capsid is 200-450nm in length, and 30-100nm in diameter. The capsid has helical symmetry. When the baculoviruses are extracellular, they can be found in two forms: budded virus (BV) and occluded virus (OV). OVs are polyhedral or oval-shaped crystalline protein matrices in which one or several mature virions are embedded. The OVs are large, measuring 0.15-15μm in length. OV particles are formed inside infected cells and are released when the cell lyses. The crystalline protein matrix of the OVprotects the virus while in the extracellular environment; because of this, OVs are used for transfer of the virus between hosts. The two genera in the family Baculoviridae are definied by their different OV structure. Granulovirus OVs contain only one virion, and do not have a polyhedral envelope (known as a calyx). These OV are small, giving a granular appearance when many OVs are seen together. ...
Recombinant H1N1 Hemagglutinin/HA Protein (Met1-Gln529) 40464-V08B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this H1N1 Hemagglutinin/HA protein for relevant bioassay and related research.
Recombinant H3N2 Hemagglutinin/HA Protein (Met1-Trp530) 40154-V08B with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this H3N2 Hemagglutinin/HA protein for relevant bioassay and related research.
H6N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate 40166-V08BL is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
H3N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
H3N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
Human CSF1R Baculovirus-Insect cells Overexpression Lysate | SinoBiological, 10161-H20B2L is used as western blot (WB) positive control. Bulk order, Produced in house, High quality guarrantee.
DATE: April 28, 2020 TIME: 9:00am PT, 12:00pm ET The Baculovirus Expression Vector System is a versatile platform for expression of individual recombinant proteins, membrane proteins, virus-
Murine tissue inhibitor of metalloproteinases-1 (mTIMP-1) was expressed in baculovirus-infected insect cells (Sf9). The protein secreted into the culture medium was purified to homogeneity by means of heparin-Sepharose CL-6B and FPLC. The purified protein showed metalloproteinase-inhibitory activity in two independent assays: reverse zymography and inhibition of collagenase activity. Digestion of the recombinant TIMP-1 with peptide-N-glycanaseF revealed that both N-glycosylation sites are used. I-125-mTIMP-1 intraveneously injected into a male Sprague Dawley rat disappeared within 2 min from the circulation. 5 min after injection more than 50% of the I-125-mTIMP-1 were found in the liver and 20% in the kidneys. At later times, trichloroacetic-acid-soluble material accumulated in the intestinal tract ...
The invention relates to a modified baculovirus wherein one of the two strong late promoters of the wild baculovirus is inactive, as well as to a method for obtaining such a modified baculovirus and to its application for obtaining vectors for the expression of exogenous genes. Said modified baculovirus is particularly deprived of the polyhedrin gene promoter and contains the protein P10 gene promoter.
Murphy, C. I., Piwnica-Worms, H., Grünwald, S., Romanow, W. G., Francis, N. and Fan, H.-Y. 2004. Maintenance of Insect Cell Cultures and Generation of Recombinant Baculoviruses. Current Protocols in Molecular Biology. 65:II:16.10:16.10.1-16.10.19. ...
From BioPortfolio: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recogn...
Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortening the time required for infection to kill or incapacitate insect pests and reducing the quantity of crop damage as a consequence. A wide variety of neurotoxic peptides, proteins that regulate insect physiology, degradative enzymes, and other potentially insecticidal proteins have been evaluated for their capacity to reduce the survival time of baculovirus-infected lepidopteran host larvae. Researchers have investigated the factors involved in the efficient expression and delivery of baculovirus-encoded insecticidal peptides and proteins, with much effort dedicated to identifying ideal promoters for driving transcription and signal peptides that mediate secretion of the expressed target
The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found in Escherichia coli expressing the recombinant CP gene ...
Recombinant IKK-α and IKK-β were purified from baculovirus-infected insect cells (Fig. 1 A and B). Both proteins directly phosphorylate Ser-32 and -36 of IκBα and Ser-19 and -23 of IκBβ in vitro and are, therefore, bona fide IκB kinases. That being said, given the homology between the NF-κB pathway in mammals and the Dif immune response pathway in Drosophila (31), it is conceivable that in vivo these overexpressed IKKs (and by analogy, the insect cell IKK homologues) might exist as high molecular weight complexes in insect cells, just as they do in mammalian cells (11-13). Furthermore, association with other proteins might be necessary to achieve activation. Although the data imply that IKK-α/IKK-β heterodimerization is not obligatory for activity, it is conceivable that heterodimerization might modulate IKK-α or IKK-β activity and/or substrate specificity.. Recombinant IKK-α and IKK-β purified from baculovirus-infected Sf9 cells are active enzymes (Fig. 1). One possible reason for ...
There are many methods presently available to produce recombinant proteins in mammalian systems. The BacMam system is a simple straightforward method which overlaps two well-established technologies,...
Publications within this content type and its collections are copyrighted content. Content can be downloaded and printed for personal use. Usage without proper citations and commercial use is prohibited.. ...
The Viral Genomes Resource is a collection of viral genomic sequences that is a part of the Entrez Genomes, which provides curated sequence data and annotations of complete genomes to the scientific community.
Screening of anti-MUC1 antibodies for reactivity with native (ascites) and recombinant (baculovirus) MUC1 and for blocking MUC1 specific cytotoxic T-lymphocytes Article Conference Paper ...
Methods to induce antigen‐specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit
Antibodies: A Laboratory Manual, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. This second edition of the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies.
ViroTag® BCVB (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on the baculovirus particle. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take baculovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag BCVB kit (catalog number 92108) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
Insect cell‐recombinant baculovirus co‐cultures offer a protein production system that complements microbial systems by providing recombinant proteins in soluble form and with most post‐translational modifications
For parallel expression of His-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a single construct
Haining R.L., Hunter A.P., Veronese M.E., Trager W.F., Rettie A.E. (1996). Allelic variants of human cytochrome P450 2C9: baculovirus-mediated expression, purification, structural characterization, substrate stereoselectivity, and prochiral selectivity of the wild-type and I359L mutant forms.. Arch. Biochem. Biophys. 333: 447 - 458. PubMed DOI:10.1006/abbi.1996.0414 ...
BioAssay record AID 725667 submitted by ChEMBL: Inhibition of C-terminal His-tagged human recombinant HDAC9 (amino acids 604- 1066) expressed in baculovirus expression system using fluorogenic acetylated peptide as substrate preincubated with enzyme for 10 mins prior to substrate addition measured after 60 mins by fluorescence analysis.
Human ACO1 / irp1 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Sino Biological produced high purity EGFR proteins in house covering various species Human, Mouse, Rat, Cynomolgus, Rhesus, Canine and with different tags which are expressed in HEK293 Cells, Baculovirus-Insect Cells, CHO Stable Cells.
Scientific American is the essential guide to the most awe-inspiring advances in science and technology, explaining how they change our understanding of the world and shape our lives.
The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in any other protein expression system, it is important to improve the production capabilities of this vector. The orf46 viral gene was identified among the most highly abundant sequences in the transcriptome of Spodoptera exigua larvae infected with its native baculovirus, the S. exigua multiple nucleopolyhedrovirus (SeMNPV). Different sequences upstream of the orf46 gene were cloned, and their promoter activities were tested by the expression of the GFP reporter gene using the Autographa californica nucleopolyhedrovirus (AcMNPV) vector system in different insect cell lines (Sf21, Se301, and Hi5) and in larvae from S. exigua and Trichoplusia ni. The strongest promoter activity was defined by a 120 nt sequence upstream of the ATG start codon for the
Respiratory disease is a major cause of economic losses for the swine industry and ranks as a primary contributor to mortality in swine. Over the last 20 years, as swine production operations have continued to consolidate, respiratory disease in swine has become increasingly multifactorial in nature. The work presented in this dissertation centers around the development of vaccines utilizing the baculovirus expression vector system (BEVS) for two viral pathogens associated with the Porcine Respiratory Disease Complex (PRDC), a multifactorial respiratory disease syndrome affecting growing and finishing phase pigs. The work in this dissertation demonstrates that vaccines formulated with subunit hemagglutinin (HA) fused to an IgG Fc domain, VLP-displayed HA, or baculovirus-displayed HA may be viable alternatives to the traditional inactivated whole virus approaches currently utilized in the veterinary vaccine industry. In addition, this work uncovers a potential problem for the development of VLP-based
We have found, unexpectedly, that the baculovirus polyhedrin is strikingly similar in structure to a picornavirus coat protein, at a level suggestive of homology. It is indeed feasible that these two molecules share a common ancestor, as there is a precedent for lateral gene transfer between baculoviruses and RNA viruses (e.g. gp64 of baculoviruses and gp75 of thogotovirus; Kadlec et al, 2008) and the insect picornavirus cricket paralysis virus resembles a primitive picornavirus (no cellular proteins show such a strong similarity to the baculovirus poyhedrin, Figure 3). This suggests that either the picornavirus‐like capsids or the baculovirus polyhedrin might have originated through co‐infection of an insect. The latter seems the more attractive hypothesis, as the picornavirus superfamily includes, for instance, plant viruses, which are likely more ancient (Figure 3), but are less similar to AcMNPV polyhedrin. Furthermore, although polyhedra are not required for viral transmission, the ...
The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccination vector with several benefits, such as its low clinical risks and posttranslational modification ability. We recently reported that dendritic cells (DCs) infected with BV stimulated antitumor immunity. The recombinant BV (rBV) also strongly stimulated peptide-specific T-cells and antitumor immunity. In this study, the stimulation of an immune response against EG7-OVA tumors in mice by a recombinant baculovirus-based combination vaccine expressing fragment C-ovalbumin (FrC-OVA-BV; rBV) was evaluated. We constructed an rBV expressing fragment C (FrC) of tetanus toxin containing a promiscuous MHC II-binding sequence and a p30-ovalbumin (OVA) peptide that functions in the MHC I pathway. The results showed that rBV activated the CD8+ T-cell-mediated response much more efficiently than the wild-type
Dear all, I am about to express MFP in a baculovirus system. The transfer vector system is based on the pVL1393 from Pharmigen. As it happens, our lab has a stock of transfection material from Clontech, who uses the pBacPAK series of vectors as transfer vectors. My question is therefore very simple, is the transfection mix the same in both cases or do you need to buy the Pharmigen baculogold kit to get the virus ?? Thanks in advance for any input, Svend -- *************************************** Svend Kjær, Ph.D. Division of Molecular Neurobiology, Department of Neuroscience Karolinska Institute Retzius väg 8, A2:2 171 77 Stockholm Sweden Tel: +46-8-7287666 Fax: +46-8-339548 Private phone: +46-8-6409962 ...
Abstract. As with any other biological based production process, batch-to-batch variability during rAAV production using baculovirus/Sf9 requires careful control and monitoring. Asa critical raw material, Baculovirus inoculum exerts a strong impact on process yield and/or product quality. Each baculovirus bank requires extensive analytical characterization and production parameters are often re-optimized for each bank, adding manufacturing time and cost. Executing multiple large scale rAAV production runs from a single inoculum would create significant manufacturing value.. Voyager Therapeutics has advanced a scalable and robust high cell density perfusion baculovirus/Sf9 platform to manufacture Baculovirus Infected Insect Cells (BIICs) for use as virus inoculum banks. An Improved flow system promotes high cell density and enables media exchange prior to BIIC cell cryopreservation. This work demonstrates the strength of the Voyager Therapeutics baculovirus/Sf9 platform, its potential to enable ...
Baculovirus and Insect Cell Expression Protocols 2016 (Hardcover, 3rd Revised edition) / Editor: David W. Murhammer ; 9781493930425 ; Medical microbiology & virology, Pathology, Other branches of medicine, Medicine, Books
Full-length cDNA coding HCV core protein was isolated from human HCV positiveserum by RT-PCR and inserted into baculovirus transfer vector.Sf9 insect cells were co-transfect-ed with the recombinant transfer vector plasmid and wild type baculovirus DNA.The recombi-nant baculoviruses containing gene of HCV core protein were obtained by plaque screening.Therecombinant HCV core proteins(20 kD)were expressed by Sf9 cells infected with recombinantviruses.Western blot and EIA revealed that the recombinant proteirls can be recognized by humanHCV positive sera,and the recombinant protein ean elicited specific anti-HCV antibody in ani-mals.
Hola, in my opinion the expression system will be dependent of the complexity of protein and the postranslational modifications. In the kinases, the PIM family around 30Kd is well expressed in E.coli, soluble and with activity.In the case of the PI3K complex 110 and (85 or 30Kd) if you try in E coli, probably express it in inclusion bodies, and renaturalization probably will give low yield of active protein. One more fact,all the active commercial human proteins are expresed in insect cells with 6His-or GST tags. There are some pages in internet which predicts the solubility of your recombinant protein after introducing the sequence. I would know the % of probability of solubility and if it were high, I will try in bacteria, if no probably it would insoluble in any system but I would try a secretion system. Probably more forers will give you more ideas better than mines. Buena suerte y Feliz Navidad. ...
Nonlytic insect cell expression systems are underutilized for production, cellular trafficking/localization, and recombinant protein...
Human Recombinant Enzyme Assay. Compounds were evaluated for selectivity of inhibition in vitro using baculovirus-expressed recombinant human COX-1 and COX-2 enzymes as previously described (Gierse et al., 1995). For COX-2-selective inhibitors, IC50s were generated from a mean of at least four separate determinations, and NSAIDs for comparison purposes only were tested once. Inactivation rate constants were determined as previously described (Walker et al., 2001) by measuring oxygen consumption directly with a Clark-style polarographic electrode. In addition, kinetic constants were determined by measuring the cyclooxygenase activity indirectly by utilizing tetramethyl-p-phenylenediamine as a cosubstrate with arachidonic acid by the method previously described (Gierse et al., 1999).. Radioligand Binding Assay. A direct binding assay that measures radiolabeled inhibitor binding to enzyme was developed to assess binding directly to COX-1 and COX-2 without the confounding influence of enzyme ...
Materials. Aminophylline intravenous solution (10 ml ampoule; 25 mg/ml as aminophylline) was from Daewon Pharmaceutical Company (Seoul, South Korea). 1,3-DMU, β-hydroxyethyltheophylline [internal standard for the high-performance liquid chromatographic (HPLC) analysis of theophylline and 1,3-DMU], KPLPS (purified by phenol extraction; protein ,3%), β-actin, primary monoclonal antibody for β-actin, EDTA (as a disodium salt), and Kodak X-OMAT film were purchased from Sigma-Aldrich (St. Louis, MO). Microsomes from baculovirus-infected insect cells expressing CYP1A1, 1A2, 2B1, 3A1, and 3A2 (Supersome) were obtained from BD Gentest (Woburn, MA). Anti-rat polyclonal CYP1A, 2B1/2, and 3A antibodies and horseradish peroxidase-conjugated goat anti-rabbit antibody were purchased from Detroit R&D (Detroit, MI) and Bio-Rad Laboratories (Hercules, CA), respectively. Enhanced chemiluminescence reagents were products from Amersham Biosciences Corporation (Piscataway, NJ). Other chemicals were of reagent or ...
Chymases (EC 3.4.21.39) are mast cell serine proteinases that are variably expressed in different species and, in most cases, display either chymotryptic or elastolytic substrate specificity. Given that chymase inhibitors have emerged as potential therapeutic agents for treating various inflammatory, allergic, and cardiovascular disorders, it is important to understand interspecies differences of the enzymes as well as the behavior of inhibitors with them. We have expressed chymases from humans, macaques, dogs, sheep (MCP2 and MCP3), guinea pigs, and hamsters (HAM1 and HAM2) in baculovirus-infected insect cells. The enzymes were purified and characterized with kinetic constants by using chromogenic substrates. We evaluated in vitro the potency of five nonpeptide inhibitors, originally targeted against human chymase. The inhibitors exhibited remarkable cross-species variation of sensitivity, with the greatest potency observed against human and macaque chymases, with K(i) values ranging from ...
Characterization of two human cAMP-specific phosphodiesterase subtypes expressed in baculovirus-infected insect cells. (pages 477-484). Bernard Y. Amegadzie, Charles R. Hanning, Megan M. McLaughlin, Miriam Burman, Lenora B. Cieslinski, George P. Livi and Theodore J. Torphy. Version of Record online: 2 JAN 2013 , DOI: 10.1006/cbir.1995.1091. ...
[Insect Cell Culture Medium: WakoVAC PSFM-J1] .We manufacture and distribute chemical reagents for research use only or various antibodies.
Development of a stable expression vector system for gene transfer into sceletal muscle followinq detection of sequence instability in utrophin cDNA during cloning ...
This ebook Insect Cell Cultures:: Fundamental and Applied Aspects (Current Applications sent struggled by the Firebase knowledge Interface. platform anyhow to pay to this classrooms large polynomialp. New Feature: You can only rely new star4 carols on your computer!
Anti-G Protein Gq / 11a Subunit, Internal Sequence, a.a. 115-133 Polyclonal Antibody, Unconjugated from CHEMICON,Recognizes the 41 and 43 kDa G-proteins present in liver plasma membranes and Gq/11alpha expressed in the Sf9 insect cell expression system. Negative for recombinant G(I)alpha subtypes.,biological,biology supply,biology supplies,biology product
GST Expression and Purification Kit from BD Biosciences Pharmingen,pAcGHLT-A,B,C Baculovirus Transfer Vector Set,pAcGHLT-XylE Baculovirus Control Vector,Thrombin Powder,Thrombin Dilution Buffer,Protease Inhibitor Cocktail,Insect Cell Lysis Buffer ,Glutathione Powder,GST Elution Buffer,PBS Wash Buffer,Transfection Buffer,biological,biology supply,biology supplies,biology product
pOET2C 6xHis is a baculovirus transfer vector under control of the AcMNPV polyhedron (polh) promoter with additional C-terminal 6xHis-Tag fusion sequence
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
Sino Biological produced high purity Insulin Receptor proteins in house covering various species Human and with different tags which are expressed in HEK293 Cells, Baculovirus-Insect Cells.
Rho-kinase II (ROCK-II) is a serine/threonine kinase that is involved in regulation of smooth muscle contraction and has been shown to contribute to the early stages of axon formation in neurons and the regulation of the neuronal cytoskeleton. Much of what is known about Rho-kinase function comes from cell-biological studies, whereas a paucity of biochemical characterization exists for the enzyme. In an effort to characterize ROCK-II biochemically we have cloned a truncated form of human ROCK-II comprising amino acids 1-543 and overexpressed it in Sf-21 cells. Utilizing the Sf-21/baculovirus expression system we isolated milligram quantities of ROCK-II (1-543) and purified the enzyme to near homogeneity. Optimal expression conditions revealed that infection of Sf-21 cells at a multiplicity of infection of 10 for 72h yielded maximal protein expression. Expression of ROCK-II (1-543) as an N-terminal Flag fusion protein allowed a single-step purification yielding greater than 90% homogeneous ...
Avian H7N9 influenza virus infection with fatal outcomes continues to pose a pandemic threat and highly immunogenic vaccines are urgently needed. In this report we show that baculovirus-derived recombinant H7 hemagglutinin protein, when delivered with RIG-I ligand, induced enhanced antibody and T cell responses and conferred protection against lethal challenge with a homologous H7N9 virus. These findings indicate the potential utility of RIG-I ligands as vaccine adjuvants to increase the immunogenicity of recombinant H7 hemagglutinin ...
Insect cell culture is widely used as an industrial platform in recombinant protein production for research and diagnostics (1, 3,4,5). Many commercial yeastolate products are intended as nutritional supplements for not only insect cell culture, but also for mammalian cell culture and bacterial fermentation applications (6,7,8,9,10,11,12,13,14,15). Yeastolate plays an important role in modern biological production because it can be very effective in promoting insect cell growth and enhancing production of recombinant proteins. The product is known to contain amino acids,…. ...
CORVELLO, C M; METZ, R; BRAVO, R; ARMELIN, M C S. Expression and characterization of mouse cfos protein using the baculovirus expression system: ability to form functional ap-1 complex with co-expressed c jun protein. Cellular and Molecular Biology Research, New York, v. 41, p. 527-35, 1996 ...
ii) the conversion of compounds of formula I or its MES its pharmaceutically acceptable MES.. In addition to use in the last major stage of the sequence of preparation, the General methods described above for preparing compounds of this invention may also be used for introduction of the desired groups at intermediate stages of the preparation of the desired compounds. Therefore, you should note that in such multi-stage processes the sequence of reactions should be selected so that the conditions of the reactions did not affect the existing groups in the molecule, which is desired in the final product.. The compounds of formula I and intermediate II - XI indicated in schemes I and II can be cleaned by known conventional methods, for example by chromatography or crystallization.. The In vitro tests.. 5-Reductase steroids.. The enzymatic activity can be determined using microsomes obtained from 1) the tissue is recombinantly baculovirus SF9 cells, which produce human 5-reductase type 1; or 3) ...
Expression Systems Production Boost Additive is a nutrient boost designed to increase protein expression by supporting the increased needs of insect cells infected by recombinant baculovirus. Production Boost Additive works to extend the late and very-late expression period.
BioAssay record AID 445124 submitted by ChEMBL: Inhibition of human recombinant HDAC1 expressed in baculovirus assessed as residual activity at 5 uM.
FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Recombinant human CAMK2gamma (c-terminal truncation) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Species :Human Tag :GST tag Expression System:Sf9 insect cells using baculovirus Sequence :c-terminal truncation Genbank Number :NM_172169 Sp
Recombinant fragment corresponding to amino acids 142-481 of Human AKT2 with N-terminal His tag expressed in a Baculovirus infected Sf9 cell expression system; 340 amino acids, MW 41kDa.
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Buy our Recombinant human MAP3K9 protein. Ab125554 is an active protein fragment produced in Baculovirus infected Sf9 cells and has been validated in WB…
Buy our Recombinant human ULK2 protein. Ab125622 is an active protein fragment produced in Baculovirus infected Sf9 cells and has been validated in WB, FuncS…
Education: MA, human nutrition, Simmons College, Boston; MA, nutritional sciences, Tufts University, Medford, MA; PhD, nutrition and immunology, Tufts University.. Publications. CV. ...
"ICTV Report Baculoviridae". "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV Report: ... Gammabaculovirus is a genus of viruses, in the family Baculoviridae. Hymenoptera serve as natural hosts. There are currently ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. " ...
... is a genus of viruses in the family Baculoviridae. Its natural hosts are invertebrates, among them winged ... "ICTV Report". ICTV Report Baculoviridae. "Viral Zone". ExPASy. Retrieved 12 June 2015. CS1 maint: discouraged parameter (link) ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. " ...
"Baculoviridae ~ ViralZone page". viralzone.expasy.org. Retrieved 2020-05-10. Lupiani B, Raina AK, Huber C (January 1999). " ...
Familie Baculoviridae. *Genus Alphabaculovirus. *Genus Betabaculovirus. *Genus Gammabaculovirus. *Genus Deltabaculovirus. * ...
CS1 maint: discouraged parameter (link) "ICTV Report Baculoviridae". ICTV Report: Baculoviridae Viralzone: Deltabaculovirus. ... Deltabaculovirus is a genus of viruses, in the family Baculoviridae. Mosquito larvae serve as natural hosts. There is currently ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ... Baculoviridae Genus: Deltabaculovirus Culex nigripalpus nucleopolyhedrovirus Viruses in Deltabaculovirus are enveloped. Genomes ...
... is a genus of viruses, in the family Baculoviridae. Arthropods serve as natural hosts. There are currently 26 ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Betabaculovirus Adoxophyes orana granulovirus Agrotis segetum ... CS1 maint: discouraged parameter (link) ICTV Report: Baculoviridae Viralzone: Betabaculovirus. ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ...
There are two genera in the Baculoviridae: Nucleopolyhedrovirus and Granulovirus. Of these, a granulovirus species specific to ...
Famili Baculoviridae. *Famili Coccolithoviridae. *Famili Corticoviridae. *Famili Fuselloviridae. *Famili Guttaviridae. *Famili ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
Baculoviridae and Rickettsia species infect nursery web spiders, as well. They most likely enter the gastrointestinal tract via ...
... (CpGV) is a granulovirus belonging to the family Baculoviridae. It has a double-stranded DNA ...
They are members of the Baculoviridae, a family that is restricted to insects. Most baculovirus isolates have been obtained ...
... (AcMNPV) is a nucleopolyhedrovirus belonging to the family Baculoviridae. ...
From the family Baculoviridae, it is a type of Alphabaculovirus and its genome is 80-180kb long. NPVs are commonly used as ...
Viruses from the family Baculoviridae induce in their hosts changes to both feeding behavior and environment selection. They ...
Nudiviruses are related to members of the family Baculoviridae and have orthologs of many core genes in common. Order: ...
... hytrosaviruses are similar to members of other arthropod-infecting virus families such as Baculoviridae, Nudiviridae and ...
The NPVs come under the family of baculoviridae and its virions are enveloped rod shaped nucleocapsids containing circular, ...
Since baculoviridae infect only insects and not humans, the function of P35 in the immune evasion of infected cells is not ...
Both in America and in Europe, research continues into biological control of the species, and for example the Baculoviridae ...
Baculoviridae), respectively. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (2011) ISBN ...
Superkingdom Viruses No classification - dsDNA viruses, no RNA stage Family - Baculoviridae Genus - Alphabaculovirus Species - ...
Baculoviridae, Bicaudaviridae, Clavaviridae, Fuselloviridae, Globuloviridae, Guttaviridae, Halspiviridae, Hytrosaviridae, ...
Alphasatellitidae Ampullaviridae Anelloviridae Avsunviroidae Baculoviridae Bicaudaviridae Clavaviridae Finnlakeviridae ...
... but the term now refers to 35 species of the family Baculoviridae-mostly alphabaculoviruses, but also one deltabaculovirus and ...
... where she studied the expression of insect cells using baculoviridae. In 1992 Jansen moved back to the United States, where she ...
Artoviridae Ascoviridae Asfarviridae Aspiviridae Astroviridae Autographiviridae Avsunviroidae Bacilladnaviridae Baculoviridae ...
Baculoviridae, Bicaudaviridae, Clavaviridae, Fuselloviridae, Globuloviridae, Guttaviridae, Halspiviridae, Hytrosaviridae, ...
ISBN 978-0-306-45641-1. ICTV Report: Baculoviridae Viralzone: Baculoviridae Index of Viruses - Baculoviridae (2006). In: ICTVdB ... Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, and diptera serve as natural hosts. There are ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ... Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ipsilon multiple nucleopolyhedrovirus ...
unclassified Baculoviridae Urbanus proteus nucleopolyhedrovirus NC_029997. complete. isolate:Southern Brazil. -. 105555 nt. 119 ...
ISBN 978-0-306-45641-1. ICTV Report: Baculoviridae Viralzone: Baculoviridae Index of Viruses - Baculoviridae (2006). In: ICTVdB ... Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, and diptera serve as natural hosts. There are ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ... Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ipsilon multiple nucleopolyhedrovirus ...
Baculoviridae. From MicrobeWiki, the student-edited microbiology resource. Revision as of 18:57, 26 June 2006 by Mschlemm. ( ... The two genera in the family Baculoviridae are definied by their different OV structure. Granulovirus OVs contain only one ... There is some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. The reasons for ... Retrieved from "https://microbewiki.kenyon.edu/index.php?title=Baculoviridae&oldid=4492" ...
Baculoviridae. Chapter contents. Posted June 2018. Baculoviridae: The family. *Citation, Summary, Virion, Genome, Antigenicity ... Figure 3.Baculoviridae. Phylogeny of family Baculoviridae. The maximum likelihood tree, based on the alignment of 38 core gene ... Nucleocapsids contain one molecule of circular, supercoiled dsDNA of 80-180 kbp (Figure 1.Baculoviridae, Figure 2.Baculoviridae ... The Baculoviridae is a family of large, insect-specific viruses with circular dsDNA genomes ranging from 80 to 180 kbp. Virions ...
This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Baculoviridae, ... which is available at www.ictv.global/report/baculoviridae. ... The family Baculoviridae comprises large viruses with circular ... 4. Tanada Y, Hess RT. Baculoviridae. In Adams JR, Bonami JR. (editors) Atlas of Invertebrate Viruses Boca Raton, FL: CRC Press ... ICTV Virus Taxonomy Profile: Baculoviridae, Page 1 of 1 , Previous page , Next page , /docserver/preview/fulltext/jgv/99/9/1185 ...
The nucleocapsids are enveloped singly, and multiple virions are assembled into each OB (Figure 1.Gammabaculovirus.). The virus is restricted to the host midgut and causes what was previously described in the literature as "infectious diarrhea". Genome sequencing analyses of three viruses - Neodiprion lecontei nucleopolyhedrovirus (NeleNPV), Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), and Neodiprion abietis nucleopolyhedrovirus (NeabNPV) - revealed that these viruses do not encode typical envelope fusion proteins found in other baculoviruses (Arif et al., 2011). This observation has raised the question of whether the budded virus phenotype plays a role in gammabaculovirus biology.. ...
title = "ICTV virus taxonomy profile: Baculoviridae",. abstract = "The family Baculoviridae comprises large viruses with ... The family Baculoviridae comprises large viruses with circular dsDNA genomes ranging from 80 to 180 kbp. The virions consist of ... N2 - The family Baculoviridae comprises large viruses with circular dsDNA genomes ranging from 80 to 180 kbp. The virions ... AB - The family Baculoviridae comprises large viruses with circular dsDNA genomes ranging from 80 to 180 kbp. The virions ...
Family Baculoviridae-() Harrison, R.L., Herniou, E.A., Jehle, J.A., Theilman, D.A., Burand, J.P., Becnel, J.J., Krell, P.J., ... Baculoviridae-() Herniou, E., Theilmann, D.A., Blissard, G.W., Becnel, J.J., Basil, A., Harrison, R.L., Bonning, B.C., Vlak, J. ... ICTV virus taxonomy profile: Baculoviridae-() Harrison, R.L., Herniou, E.A., Jehle, J.A., Theilmann, D.A., Burand, J.P., Becnel ... Baculoviridae. Virus Taxonomy: Classification and Nomenclature of Viruses. oxford:Elsevier 163-174.. ...
Baculoviridae - Herniou, E., Theilmann, D.A., Blissard, G.W., Becnel, J.J., Basil, A., Harrison, R.L., Bonning, B.C., Vlak, J.M ... Baculoviridae. Virus Taxonomy: Classification and Nomenclature of Viruses. oxford:Elsevier 163-174. ...
The present invention relates to detecting target nucleic acid sequences in pooled samples. In particular, the present invention relates to compositions and methods for detecting the presence or absence of target nucleic acid sequences (e.g. RNA virus sequences) in a pooled sample employing an INVADER detection assay. In certain embodiments, the present invention allows target nucleic acid sequence detection in pooled biological samples (e.g. pooled blood samples) without prior amplification of the target.
Familie Baculoviridae. *Genus Alphabaculovirus. *Genus Betabaculovirus. *Genus Gammabaculovirus. *Genus Deltabaculovirus. * ...
Baculoviridae, Filoviridae, Flaviviridae, Arenaviridae, Rhabdoviridae, Paramyxoviridae, and Coronaviridae [19, 20] (Table 3.1 ...
Baculoviridae. Genus. Alphabaculovirus. Genus. Betabaculovirus. Genus. Gammabaculovirus. Genus. Deltabaculovirus. Family. ...
Processing of the NF-kappa B2 precursor p100 to the mature p52 subunit is regulated via a unique pathway. NF-kappa B-inducing kinase (NIK) induces I kappa B kinase alpha (IKK alpha)-mediated phosphorylation of specific serine residues in the C-terminal domain of p100, leading to recruitment of the S …
There are two genera in the Baculoviridae: Nucleopolyhedrovirus and Granulovirus. Of these, a granulovirus species specific to ...
Baculoviridae:. Nucleopolyhedrovirus. Autographa californica nucleopolyhedrovirus. Invertebrates. Granulovirus. Plodia ...
"ICTV Report Baculoviridae". "Viral Zone". ExPASy. Retrieved 15 June 2015. CS1 maint: discouraged parameter (link) ICTV Report: ... Gammabaculovirus is a genus of viruses, in the family Baculoviridae. Hymenoptera serve as natural hosts. There are currently ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. " ...
Famili Baculoviridae. *Famili Coccolithoviridae. *Famili Corticoviridae. *Famili Fuselloviridae. *Famili Guttaviridae. *Famili ...
Baculoviridae / genetics * Base Sequence * Caliciviridae Infections / epidemiology * Caliciviridae Infections / microbiology* * ...
Baculoviridae (e.g. insect baculovirus). *Hepadnaviridae (e.g. Hepatitis B). *Herpesviridae (e.g. herpesviruses, chickenpox ( ...
Baculoviridae; Alphabaculovirus. OX NCBI_TaxID=46015; OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths). RN [1] RP ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
Baculoviridae: Nuclear Polyhedrosis Viruses. Part I. Nuclear Polyhedrosis Viruses of Insects. In Atlas of Invertebrate Viruses ...
They are members of the Baculoviridae, a family that is restricted to insects. Most baculovirus isolates have been obtained ...
Granuloviruses are in the family of insect viruses called the baculoviridae. These viruses target insects and are popular as ... Extensive research is being done on more uses and applications of baculoviridae to operate as pesticides against more ...
Family Baculoviridae. In: van Regenmoertel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B.,Estes, M.K., Lemon, S.M., ...
3). For example, viruses belonging to the family Baculoviridae branched from the Asfarviridae outgroup with a likelihood ... Baculoviridae, and Herpesviridae. Several sequences from DGGE (SO98-1, SO98-2, SO98-3, BSA99-2, BSA99-5, SIA99-1, MIB99-2, and ... Baculoviridae; H, Herpesviridae; P, Phycodnaviridae. The Phycodnaviridae are also indicated in boldface lettering. The scale ... viruses from the families Baculoviridae and Herpesviridae all clustered with viruses of the same family. ...
2005 Family Baculoviridae. In Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses (eds Fauquet ...
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  • Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus Neodiprion sertifer nucleopolyhedrovirus Viruses in Gammabaculovirus are enveloped. (wikipedia.org)
  • This effect was studied in vitro during lepidopteran-specific nucleopolyhedrovirus (genus Alphabaculovirus, family Baculoviridae) infection. (brookes.ac.uk)
  • The most common viruses of mosquitoes are the baculoviruses (NPVs) (Baculoviridae: Nucleopolyhedrovirus) and cytoplasmic polyhedrosis viruses (CPVs) (Reoviridae: Cypovirus). (bioone.org)
  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the type species for the genus Alphabaculovirus in the family Baculoviridae. (gc.ca)
  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a prototype of the Baculoviridae family, is a promising new vector candidate for gene therapy. (jyu.fi)
  • Field trials and assessments of the balsam fir sawfly ( Neodiprion abietis) nucleopolyhedrovirus (NeabNPV: Baculoviridae, Gammabaculovirus) against its natural host were conducted in July and August 2002 near Corner Brook, Newfoundland and Labrador, Canada, in naturally regenerated, precommercially thinned stands dominated by balsam fir ( Abies balsamea). (oalib.com)
  • Baculoviridae is a family of viruses. (wikipedia.org)
  • The Baculoviridae is a family of large, insect-specific viruses with circular dsDNA genomes ranging from 80 to 180 kbp. (ictvonline.org)
  • This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Baculoviridae , which is available at www.ictv.global/report/baculoviridae . (microbiologyresearch.org)
  • My research focuses mostly on a group of insect-specific viruses known as baculoviruses, which are classified in the family Baculoviridae . (usda.gov)
  • Gammabaculovirus is a genus of viruses, in the family Baculoviridae. (wikipedia.org)
  • Granuloviruses are in the family of insect viruses called the baculoviridae. (kenyon.edu)
  • In conclusion, the sequence differences between the Hz-1 virus and the baculoviruses outnumber the similarities and suggest that the Hz-1 virus may form a new family of viruses distantly related to the Baculoviridae . (asm.org)
  • were sampled extensively at three locations in New Brunswick, Canada, between 1982 and 1992 and were examined for the prevalence of granulosis and nuclear polyhedrosis viruses (Baculoviridae). (gc.ca)
  • The coronavirus S protein belongs to the class I viral fusion proteins ( 2 ), together with the fusion proteins of viruses of the orthomyxo -, retro -, paramyxo -, filo -, arena -, and baculoviridae . (pubmedcentralcanada.ca)
  • Members of the Baculoviridae , a unique family of eukaryotic of rod-shaped enveloped viruses with large circular double stranded DNA genomes, are unique because of their production of two different phenotypes of viruses during their infection cycle, and their extreme host specificity. (queensu.ca)
  • LdMNPV is a member of the genus Alphabaculovirus , family Baculoviridae . (asmblog.org)
  • The Baculoviridae family can be divided into four genera according to common biological and structural characteristics: Alphabaculovirus, which includes lepidopteran specific baculoviruses and is subdivided into Group I or Group II based on the type of fusogenic protein, Betabaculovirus, comprising lepidopteran-specific granuloviruses, Gammabaculovirus, which includes hymenopteran-specific baculoviruses, and Deltabaculovirus, which, to date, comprises only CuniNPV and possibly still undescribed dipteran-specific baculoviruses [ 3 - 6 ]. (omicsonline.org)
  • Genus Tequatrovirus ( T4virus , T4-ähnliche Viren , en. (wikipedia.org)
  • Nucleopolyhedroviruses (NPV: Gammabaculovirus: Baculoviridae) of diprionid sawflies (Diprionidae: Hymenoptera) are highly host specific and only infect the midgut epithelium. (oalib.com)
  • Baculoviridae) of S. exigua is a native pathogen of this insect. (worldwidescience.org)
  • A baculovirus is an enveloped double-stranded DNA virus that belongs to the Baculoviridae family, which affects approximately 600 different insect species. (news-medical.net)
  • Originally classified as a member of the Baculoviridae , the Hz-1 virus is currently unclassified due to its lack of an occlusion body, as well as its low DNA homology with other baculoviruses ( 59 ). (asm.org)
  • Nuclear polyhedrosis (BmNPV) belongs to Baculoviridae, causes nuclear polyhedrosis (grasserie) in silkworms which is the most common viral disease and is prevalent in almost all the sericulture areas in India. (academicjournals.org)
  • Para el control del gusano cogollero S. frugiperda (JE Smith) (Lepidoptera: Noctuidae) en cultivos de maíz, se evaluaron los cuerpos de inclusión virales (CIs) de un nucleopoliedrovirus de Spodoptera frugiperda colombiano (SfCOL) y una de sus variantes genotípicas (SfCOL-A), formulados como polvos mojables mediante microencapsulación. (scielo.org.co)
  • Vuill, a granulosis virus (Baculoviridae: Eubaculovirinae), and a protozoan (phylum Microspora) were found. (bioone.org)
  • Baculoviridae, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. (thefreedictionary.com)
  • Hz-1 virus was classified as a member of subgroup C of the Baculoviridae and is a Nudibaculoviridae species. (asm.org)
  • There is some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. (kenyon.edu)
  • The two genera in the family Baculoviridae are definied by their different OV structure. (kenyon.edu)
  • Baculoviridae ) are generated when nucleocapsids bud through the plasma membrane at the surface of infected cells. (ictvonline.org)
  • However, low sequence homology between Hz-1 virus and other members of the Baculoviridae was observed. (asm.org)