Baculoviridae: Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.Nucleopolyhedrovirus: A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. The type species is Autographa californica nucleopolyhedrovirus.Spodoptera: A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.National Library of Medicine (U.S.): An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.History, 16th Century: Time period from 1501 through 1600 of the common era.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Hymenoptera: An extensive order of highly specialized insects including bees, wasps, and ants.Black Widow Spider: A venomous New World spider with an hourglass-shaped red mark on the abdomen.Moths: Insects of the suborder Heterocera of the order LEPIDOPTERA.Granulovirus: A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by ovicylindrical occlusion bodies. The type species is Cydia pomonella granulovirus.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Capsid: The outer protein protective shell of a virus, which protects the viral nucleic acid.Virion: The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Illusions: The misinterpretation of a real external, sensory experience.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.GermanyRed Cross: International collective of humanitarian organizations led by volunteers and guided by its Congressional Charter and the Fundamental Principles of the International Red Cross Movement, to provide relief to victims of disaster and help people prevent, prepare for, and respond to emergencies.Classification: The systematic arrangement of entities in any field into categories classes based on common characteristics such as properties, morphology, subject matter, etc.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Lentivirus: A genus of the family RETROVIRIDAE consisting of non-oncogenic retroviruses that produce multi-organ diseases characterized by long incubation periods and persistent infection. Lentiviruses are unique in that they contain open reading frames (ORFs) between the pol and env genes and in the 3' env region. Five serogroups are recognized, reflecting the mammalian hosts with which they are associated. HIV-1 is the type species.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Indigo Carmine: Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.BooksVanilla: A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).Paeonia: A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.Malus: A plant genus in the family ROSACEAE, order Rosales, subclass Rosidae. It is best known as a source of the edible fruit (apple) and is cultivated in temperate climates worldwide.Dodecanol: A saturated 12-carbon fatty alcohol obtained from coconut oil fatty acids. It has a floral odor and is used in detergents, lubricating oils, and pharmaceuticals. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Flight, Animal: The use of wings or wing-like appendages to remain aloft and move through the air.Fruit: The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.Indonesia: A republic stretching from the Indian Ocean east to New Guinea, comprising six main islands: Java, Sumatra, Bali, Kalimantan (the Indonesian portion of the island of Borneo), Sulawesi (formerly known as the Celebes) and Irian Jaya (the western part of New Guinea). Its capital is Djakarta. The ethnic groups living there are largely Chinese, Arab, Eurasian, Indian, and Pakistani; 85% of the peoples are of the Islamic faith.BaltimoreMalaysia: A parliamentary democracy with a constitutional monarch in southeast Asia, consisting of 11 states (West Malaysia) on the Malay Peninsula and two states (East Malaysia) on the island of BORNEO. It is also called the Federation of Malaysia. Its capital is Kuala Lumpur. Before 1963 it was the Union of Malaya. It reorganized in 1948 as the Federation of Malaya, becoming independent from British Malaya in 1957 and becoming Malaysia in 1963 as a federation of Malaya, Sabah, Sarawak, and Singapore (which seceded in 1965). The form Malay- probably derives from the Tamil malay, mountain, with reference to its geography. (From Webster's New Geographical Dictionary, 1988, p715 & Room, Brewer's Dictionary of Names, 1992, p329)MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.RNA Viruses: Viruses whose genetic material is RNA.Vaccinia virus: The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.

Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. (1/2544)

To understand the initiation of the transcription of protein-coding genes, we have dissected the role of the basal transcription/DNA repair factor TFIIH. Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides, we were able to analyze the role of XPB, XPD, and cdk7 subunits in the transcription reaction. Designing mutated recombinant subunits, we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase, which is dispensable, stimulates transcription and allows the CAK complex to be anchored to TFIIH. In addition, we also show that cdk7 may phosphorylate the carboxy-terminal domain (CTD) of RNA pol II in the absence of promoter opening.  (+info)

A plant 126-kDa phosphatidylinositol 4-kinase with a novel repeat structure. Cloning and functional expression in baculovirus-infected insect cells. (2/2544)

Phosphatidylinositol metabolism plays a central role in signaling pathways in animals and is also believed to be of importance in signal transduction in higher plants. We report here the molecular cloning of a cDNA encoding a previously unidentified 126-kDa phosphatidylinositol (PI) 4-kinase (AtPI4Kbeta) from the higher plant Arabidopsis thaliana. The novel protein possesses the conserved domains present in animal and yeast PI 4-kinases, namely a lipid kinase unique domain and a catalytic domain. An additional domain, approximately 300 amino acids long, containing a high percentage (46%) of charged amino acids is specific to this plant enzyme. Recombinant AtPI4Kbeta expressed in baculovirus-infected insect (Spodoptera frugiperda) cells phosphorylated phosphatidylinositol exclusively at the D4 position of the inositol ring. Recombinant protein was maximally activated by 0.6% Triton X-100 but was inhibited by adenosine with an IC50 of approximately 200 microM. Wortmannin at a concentration of 10 microM inhibited AtPI4Kbeta activity by approximately 90%. AtPI4Kbeta transcript levels were similar in all tissues analyzed. Light or treatment with hormones or salts did not change AtPI4Kbeta transcript levels to a great extent, indicating constitutive expression of the AtPI4Kbeta gene.  (+info)

Baculovirus expression and biochemical characterization of the human microsomal triglyceride transfer protein. (3/2544)

The microsomal triglyceride transfer protein (MTP) complexed to protein disulphide isomerase (PDI) is obligatory for the assembly of chylomicrons and very-low-density lipoproteins. The determination of the atomic structure of the MTP-PDI heterodimer has important implications for the treatment of those forms of hyperlipidaemia associated with the overproduction of very-low-density lipoproteins, which predispose to premature coronary heart disease. To perform structural studies of the human MTP-PDI complex it was necessary to produce milligram quantities of pure protein. We chose the baculovirus expression system for this purpose. Insects cells were co-infected with recombinant viruses encoding FLAG-tagged MTP and His-tagged PDI; the resulting heterodimer was purified by affinity chromatography. From 5 litres of insect cells, 4-6 mg of more than 95% pure recombinant protein was obtained. CD and attenuated total reflection Fourier-transform infrared spectroscopy indicate that the purified protein has around 34% alpha-helical and 33% beta-structure content. The recombinant protein had a comparable triglyceride transfer activity to that of bovine MTP-PDI. The production of polyclonal antibodies raised against the MTP and PDI subunits of the purified protein is described. The present study demonstrates the feasibility of expressing two proteins at high levels in insect cells and describes a transferable methodology for the purification of the resulting protein complex.  (+info)

A lipid modified ubiquitin is packaged into particles of several enveloped viruses. (4/2544)

An anti-ubiquitin cross-reactive protein which migrates more slowly (6.5 kDa) by SDS-PAGE than ubiquitin was identified in African swine fever virus particles. This protein was extracted into the detergent phase in Triton X-114 phase separations, showing that it is hydrophobic, and was radiolabelled with both [3H]palmitic acid and [32P]orthophosphate. This indicates that the protein has a similar structure to the membrane associated phosphatidyl ubiquitin described in baculovirus particles. A similar molecule was found in vaccinia virus and herpes simplex virus particles, suggesting that it may be a component of uninfected cell membranes, which is incorporated into membrane layers in virions during morphogenesis.  (+info)

Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus. (5/2544)

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.  (+info)

Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. (6/2544)

Although great progress has been made in the characterization of the biochemical and biological features of hepatitis C virus (HCV) gene expression, the elucidation of the HCV life cycle and the evaluation of novel antiviral strategies have been hindered by the lack of a suitable cell culture system. In this context, the development of an efficient HCV cDNA delivery method would contribute to the understanding of HCV replication. To assess the functionality of baculovirus mediated gene delivery for HCV expression, we have constructed recombinant baculoviruses encoding HCV cDNA under the control of the cytomegalovirus promoter. Transduction of the human hepatoma cell line Huh-7 with Bac-HCV vectors was efficient and HCV cDNA expression was enhanced by treatment of the infected cells with dexamethasone. HCV structural and nonstructural polypeptides were processed correctly and were found to localize in the cytoplasm in a pattern characteristic of the endoplasmic reticulum. The expression of the HCV proteins was detected for 49 days after infection. Thus, these results indicate that the recombinant Bac-HCV vectors are a useful tool for the delivery of HCV cDNA and can facilitate the analysis of structural and functional properties of the HCV proteins. In addition, the Bac-HCV vectors can provide important information on the evaluation of novel anti-HCV antiviral strategies.  (+info)

Reovirus virion-like particles obtained by recoating infectious subvirion particles with baculovirus-expressed sigma3 protein: an approach for analyzing sigma3 functions during virus entry. (7/2544)

Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a partial way for the major outer-capsid protein sigma3, we obtained in vitro assembly of large numbers of virion-like particles by binding baculovirus-expressed sigma3 protein to infectious subvirion particles (ISVPs) that lack sigma3. A level of sigma3 binding approaching 100% of that in native virions was routinely achieved. The sigma3 coat in these recoated ISVPs (rcISVPs) appeared very similar to that in virions by electron microscopy and three-dimensional image reconstruction. rcISVPs retained full infectivity in murine L cells, allowing their use to study sigma3 functions in virus entry. Upon infection, rcISVPs behaved identically to virions in showing an extended lag phase prior to exponential growth and in being inhibited from entering cells by either the weak base NH4Cl or the cysteine proteinase inhibitor E-64. rcISVPs also mimicked virions in being incapable of in vitro activation to mediate lysis of erythrocytes and transcription of the viral mRNAs. Last, rcISVPs behaved like virions in showing minor loss of infectivity at 52 degrees C. Since rcISVPs contain virion-like levels of sigma3 but contain outer-capsid protein mu1/mu1C mostly cleaved at the delta-phi junction as in ISVPs, the fact that rcISVPs behaved like virions (and not ISVPs) in all of the assays that we performed suggests that sigma3, and not the delta-phi cleavage of mu1/mu1C, determines the observed differences in behavior between virions and ISVPs. To demonstrate the applicability of rcISVPs for genetic studies of protein functions in reovirus entry (an approach that we call recoating genetics), we used chimeric sigma3 proteins to localize the primary determinants of a strain-dependent difference in sigma3 cleavage rate to a carboxy-terminal region of the ISVP-bound protein.  (+info)

Production and characterization of a soluble, active form of Tva, the subgroup A avian sarcoma and leukosis virus receptor. (8/2544)

The receptor for the subgroup A avian sarcoma and leukosis viruses [ASLV(A)] is the cellular glycoprotein Tva. A soluble form of Tva, sTva, was produced and purified with a baculovirus expression system. Using this system, 7 to 10 mg of purified sTva per liter of cultured Sf9 cells was obtained. Characterization of the carbohydrate modification of sTva revealed that the three N glycosylation sites in sTva were differentially utilized; however, the O glycosylation common to Tva produced in mammalian and avian cells was not observed. Purified sTva demonstrates significant biological activity, specifically blocking infection of avian cells by ASLV(A) with a 90% inhibitory concentration of approximately 25 pM. A quantitative enzyme-linked immunosorbent assay, developed to assess the binding of sTva to ASLV envelope glycoprotein, demonstrates that sTva has a high affinity for EnvA, with an apparent dissociation constant of approximately 0.3 nM. Once they are bound, a very stable complex is formed between EnvA and sTva, with an estimated complex half-life of 6 h. The soluble receptor protein described here represents a valuable tool for analysis of the receptor-envelope glycoprotein interaction and for structural analysis of Tva.  (+info)

GlycoBac LLC provides insect cell lines, baculovirus vectors, and plasmids for the production of recombinant proteins in the baculovirus insect cell system
Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression Vector System (BEVS). We discuss the different strategies used to generate recombinant baculovirus vectors and show how advanced techniques for virus titer determination can accelerate the production of recombinant proteins. The stable transfection of insect cells is an alternative to BEVS which has recently been augmented with recombinase-mediated cassette exchange for site-specific gene integration. We consider the advantages and limitations of these techniques for the production of recombinant proteins in insect cells and compare them to other expression platforms.
Baculovirus gene transfer into Mammalian cells, known from scientific research articles as BacMam, is the use of baculovirus to deliver genes to mammalian cells. Baculoviruses are insect cell viruses that can be modified to express proteins in mammalian cells. The unmodified baculovirus is able to enter mammalian cells, however its genes are not expressed unless a mammalian recognizable promoter is incorporated upstream of a gene of interest. Both unmodified baculovirus and baculovirus modified with a mammalian promoter (BacMam) are unable to replicate in humans and are thus non infectious. Invented by Dr. Frederick M. Boyce, BacMam is a baculovirus-mediated gene transfer technique that has gained widespread use because of advantages when compared to other transfection methods, (for reviews see, Kost, T.A. et al,). In addition, BacMam has been found to have inherent flexibility over stable cell lines, which has contributed to its adoption as a standard gene transfer technique. The BacMam gene ...
Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, diptera, and decapoda serve as natural hosts. There are currently 49 species in this family, divided among 4 genera. Baculoviruses are known to infect invertebrates, with over 600 host species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture they are not known to be capable of replication in mammalian or other vertebrate animal cells. Starting in the 1940s they were used and studied widely as biopesticides in crop fields. Baculoviruses contain circular double-stranded genome ranging from 80 to 180 kbp. The earliest records of baculoviruses can be found in the literature from as early as the sixteenth century in reports of "wilting disease" infecting silk-producing larva. Starting in the 1940s they were used and studied widely ...
Baculovirus virions have a complex structure which consists of an envelope and a rod-shaped nucleocapsid. The capsid is 200-450nm in length, and 30-100nm in diameter. The capsid has helical symmetry. When the baculoviruses are extracellular, they can be found in two forms: budded virus (BV) and occluded virus (OV). OVs are polyhedral or oval-shaped crystalline protein matrices in which one or several mature virions are embedded. The OVs are large, measuring 0.15-15μm in length. OV particles are formed inside infected cells and are released when the cell lyses. The crystalline protein matrix of the OVprotects the virus while in the extracellular environment; because of this, OVs are used for transfer of the virus between hosts. The two genera in the family Baculoviridae are definied by their different OV structure. Granulovirus OVs contain only one virion, and do not have a polyhedral envelope (known as a calyx). These OV are small, giving a "granular" appearance when many OVs are seen together. ...
H6N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate 40166-V08BL is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
H3N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
H3N2 Hemagglutinin/HA Baculovirus-Insect Cells Overexpression Lysate is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
... , 10161-H20B2L is used as western blot (WB) positive control. Bulk order, Produced in house, High quality guarrantee.
DATE: April 28, 2020 TIME: 9:00am PT, 12:00pm ET The Baculovirus Expression Vector System is a versatile platform for expression of individual recombinant proteins, membrane proteins, virus-
Murine tissue inhibitor of metalloproteinases-1 (mTIMP-1) was expressed in baculovirus-infected insect cells (Sf9). The protein secreted into the culture medium was purified to homogeneity by means of heparin-Sepharose CL-6B and FPLC. The purified protein showed metalloproteinase-inhibitory activity in two independent assays: reverse zymography and inhibition of collagenase activity. Digestion of the recombinant TIMP-1 with peptide-N-glycanaseF revealed that both N-glycosylation sites are used. I-125-mTIMP-1 intraveneously injected into a male Sprague Dawley rat disappeared within 2 min from the circulation. 5 min after injection more than 50% of the I-125-mTIMP-1 were found in the liver and 20% in the kidneys. At later times, trichloroacetic-acid-soluble material accumulated in the intestinal tract ...
The invention relates to a modified baculovirus wherein one of the two strong late promoters of the wild baculovirus is inactive, as well as to a method for obtaining such a modified baculovirus and to its application for obtaining vectors for the expression of exogenous genes. Said modified baculovirus is particularly deprived of the polyhedrin gene promoter and contains the protein P10 gene promoter.
Murphy, C. I., Piwnica-Worms, H., Grünwald, S., Romanow, W. G., Francis, N. and Fan, H.-Y. 2004. Maintenance of Insect Cell Cultures and Generation of Recombinant Baculoviruses. Current Protocols in Molecular Biology. 65:II:16.10:16.10.1-16.10.19. ...
Baculovirus Expression System, BacPAK, is a complete system for expressing fully-functional recombinant proteins at extremely high levels in insect host cells.
Baculovirus Expression System, BacPAK, is a complete system for expressing fully-functional recombinant proteins at extremely high levels in insect host cells.
From BioPortfolio: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recogn...
Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortening the time required for infection to kill or incapacitate insect pests and reducing the quantity of crop damage as a consequence. A wide variety of neurotoxic peptides, proteins that regulate insect physiology, degradative enzymes, and other potentially insecticidal proteins have been evaluated for their capacity to reduce the survival time of baculovirus-infected lepidopteran host larvae. Researchers have investigated the factors involved in the efficient expression and delivery of baculovirus-encoded insecticidal peptides and proteins, with much effort dedicated to identifying ideal promoters for driving transcription and signal peptides that mediate secretion of the expressed target
The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found in Escherichia coli expressing the recombinant CP gene ...
Recombinant IKK-α and IKK-β were purified from baculovirus-infected insect cells (Fig. 1 A and B). Both proteins directly phosphorylate Ser-32 and -36 of IκBα and Ser-19 and -23 of IκBβ in vitro and are, therefore, bona fide IκB kinases. That being said, given the homology between the NF-κB pathway in mammals and the Dif immune response pathway in Drosophila (31), it is conceivable that in vivo these overexpressed IKKs (and by analogy, the insect cell IKK homologues) might exist as high molecular weight complexes in insect cells, just as they do in mammalian cells (11-13). Furthermore, association with other proteins might be necessary to achieve activation. Although the data imply that IKK-α/IKK-β heterodimerization is not obligatory for activity, it is conceivable that heterodimerization might modulate IKK-α or IKK-β activity and/or substrate specificity.. Recombinant IKK-α and IKK-β purified from baculovirus-infected Sf9 cells are active enzymes (Fig. 1). One possible reason for ...
The Viral Genomes Resource is a collection of viral genomic sequences that is a part of the Entrez Genomes, which provides curated sequence data and annotations of complete genomes to the scientific community.
Screening of anti-MUC1 antibodies for reactivity with native (ascites) and recombinant (baculovirus) MUC1 and for blocking MUC1 specific cytotoxic T-lymphocytes Article Conference Paper ...
Methods to induce antigen‐specific immune responses in mice using insect cells infected with recombinant baculoviruses are described in this unit
Antibodies: A Laboratory Manual, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. This second edition of the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies.
ViroTag® BCVB (for manual sampling) utilizes a fluorescently-labeled, high-affinity antibody which binds to a unique epitope specifically expressed on the baculovirus particle. With the Virus Counter 3100, use this rapid, no-wash labeling procedure and take baculovirus quantification to new levels of accuracy, speed and simplicity!. Product specifications: The ViroTag BCVB kit (catalog number 92108) contains all reagents and consumables necessary to analyze 200 samples using the Virus Counter 3100 instrument for manual sampling, including:. ...
Insect cell‐recombinant baculovirus co‐cultures offer a protein production system that complements microbial systems by providing recombinant proteins in soluble form and with most post‐translational modifications
For parallel expression of His-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a single construct
Haining R.L., Hunter A.P., Veronese M.E., Trager W.F., Rettie A.E. (1996). Allelic variants of human cytochrome P450 2C9: baculovirus-mediated expression, purification, structural characterization, substrate stereoselectivity, and prochiral selectivity of the wild-type and I359L mutant forms.. Arch. Biochem. Biophys. 333: 447 - 458. PubMed DOI:10.1006/abbi.1996.0414 ...
BioAssay record AID 725667 submitted by ChEMBL: Inhibition of C-terminal His-tagged human recombinant HDAC9 (amino acids 604- 1066) expressed in baculovirus expression system using fluorogenic acetylated peptide as substrate preincubated with enzyme for 10 mins prior to substrate addition measured after 60 mins by fluorescence analysis.
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The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in any other protein expression system, it is important to improve the production capabilities of this vector. The orf46 viral gene was identified among the most highly abundant sequences in the transcriptome of Spodoptera exigua larvae infected with its native baculovirus, the S. exigua multiple nucleopolyhedrovirus (SeMNPV). Different sequences upstream of the orf46 gene were cloned, and their promoter activities were tested by the expression of the GFP reporter gene using the Autographa californica nucleopolyhedrovirus (AcMNPV) vector system in different insect cell lines (Sf21, Se301, and Hi5) and in larvae from S. exigua and Trichoplusia ni. The strongest promoter activity was defined by a 120 nt sequence upstream of the ATG start codon for the
Respiratory disease is a major cause of economic losses for the swine industry and ranks as a primary contributor to mortality in swine. Over the last 20 years, as swine production operations have continued to consolidate, respiratory disease in swine has become increasingly multifactorial in nature. The work presented in this dissertation centers around the development of vaccines utilizing the baculovirus expression vector system (BEVS) for two viral pathogens associated with the Porcine Respiratory Disease Complex (PRDC), a multifactorial respiratory disease syndrome affecting growing and finishing phase pigs. The work in this dissertation demonstrates that vaccines formulated with subunit hemagglutinin (HA) fused to an IgG Fc domain, VLP-displayed HA, or baculovirus-displayed HA may be viable alternatives to the traditional inactivated whole virus approaches currently utilized in the veterinary vaccine industry. In addition, this work uncovers a potential problem for the development of VLP-based
We have found, unexpectedly, that the baculovirus polyhedrin is strikingly similar in structure to a picornavirus coat protein, at a level suggestive of homology. It is indeed feasible that these two molecules share a common ancestor, as there is a precedent for lateral gene transfer between baculoviruses and RNA viruses (e.g. gp64 of baculoviruses and gp75 of thogotovirus; Kadlec et al, 2008) and the insect picornavirus cricket paralysis virus resembles a primitive picornavirus (no cellular proteins show such a strong similarity to the baculovirus poyhedrin, Figure 3). This suggests that either the picornavirus‐like capsids or the baculovirus polyhedrin might have originated through co‐infection of an insect. The latter seems the more attractive hypothesis, as the picornavirus superfamily includes, for instance, plant viruses, which are likely more ancient (Figure 3), but are less similar to AcMNPV polyhedrin. Furthermore, although polyhedra are not required for viral transmission, the ...
The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccination vector with several benefits, such as its low clinical risks and posttranslational modification ability. We recently reported that dendritic cells (DCs) infected with BV stimulated antitumor immunity. The recombinant BV (rBV) also strongly stimulated peptide-specific T-cells and antitumor immunity. In this study, the stimulation of an immune response against EG7-OVA tumors in mice by a recombinant baculovirus-based combination vaccine expressing fragment C-ovalbumin (FrC-OVA-BV; rBV) was evaluated. We constructed an rBV expressing fragment C (FrC) of tetanus toxin containing a promiscuous MHC II-binding sequence and a p30-ovalbumin (OVA) peptide that functions in the MHC I pathway. The results showed that rBV activated the CD8+ T-cell-mediated response much more efficiently than the wild-type
Dear all, I am about to express MFP in a baculovirus system. The transfer vector system is based on the pVL1393 from Pharmigen. As it happens, our lab has a stock of transfection material from Clontech, who uses the pBacPAK series of vectors as transfer vectors. My question is therefore very simple, is the transfection mix the same in both cases or do you need to buy the Pharmigen baculogold kit to get the virus ?? Thanks in advance for any input, Svend -- *************************************** Svend Kjær, Ph.D. Division of Molecular Neurobiology, Department of Neuroscience Karolinska Institute Retzius väg 8, A2:2 171 77 Stockholm Sweden Tel: +46-8-7287666 Fax: +46-8-339548 Private phone: +46-8-6409962 ...
Abstract. As with any other biological based production process, batch-to-batch variability during rAAV production using baculovirus/Sf9 requires careful control and monitoring. Asa critical raw material, Baculovirus inoculum exerts a strong impact on process yield and/or product quality. Each baculovirus bank requires extensive analytical characterization and production parameters are often re-optimized for each bank, adding manufacturing time and cost. Executing multiple large scale rAAV production runs from a single inoculum would create significant manufacturing value.. Voyager Therapeutics has advanced a scalable and robust high cell density perfusion baculovirus/Sf9 platform to manufacture Baculovirus Infected Insect Cells (BIICs) for use as virus inoculum banks. An Improved flow system promotes high cell density and enables media exchange prior to BIIC cell cryopreservation. This work demonstrates the strength of the Voyager Therapeutics baculovirus/Sf9 platform, its potential to enable ...
Baculovirus and Insect Cell Expression Protocols 2016 (Hardcover, 3rd Revised edition) / Editor: David W. Murhammer ; 9781493930425 ; Medical microbiology & virology, Pathology, Other branches of medicine, Medicine, Books
Full-length cDNA coding HCV core protein was isolated from human HCV positiveserum by RT-PCR and inserted into baculovirus transfer vector.Sf9 insect cells were co-transfect-ed with the recombinant transfer vector plasmid and wild type baculovirus DNA.The recombi-nant baculoviruses containing gene of HCV core protein were obtained by plaque screening.Therecombinant HCV core proteins(20 kD)were expressed by Sf9 cells infected with recombinantviruses.Western blot and EIA revealed that the recombinant proteirls can be recognized by humanHCV positive sera,and the recombinant protein ean elicited specific anti-HCV antibody in ani-mals.
Hola, in my opinion the expression system will be dependent of the complexity of protein and the postranslational modifications. In the kinases, the PIM family around 30Kd is well expressed in E.coli, soluble and with activity.In the case of the PI3K complex 110 and (85 or 30Kd) if you try in E coli, probably express it in inclusion bodies, and renaturalization probably will give low yield of active protein. One more fact,all the active commercial human proteins are expresed in insect cells with 6His-or GST tags. There are some pages in internet which predicts the solubility of your recombinant protein after introducing the sequence. I would know the % of probability of solubility and if it were high, I will try in bacteria, if no probably it would insoluble in any system but I would try a secretion system. Probably more forers will give you more ideas better than mines. Buena suerte y Feliz Navidad. ...
Human Recombinant Enzyme Assay. Compounds were evaluated for selectivity of inhibition in vitro using baculovirus-expressed recombinant human COX-1 and COX-2 enzymes as previously described (Gierse et al., 1995). For COX-2-selective inhibitors, IC50s were generated from a mean of at least four separate determinations, and NSAIDs for comparison purposes only were tested once. Inactivation rate constants were determined as previously described (Walker et al., 2001) by measuring oxygen consumption directly with a Clark-style polarographic electrode. In addition, kinetic constants were determined by measuring the cyclooxygenase activity indirectly by utilizing tetramethyl-p-phenylenediamine as a cosubstrate with arachidonic acid by the method previously described (Gierse et al., 1999).. Radioligand Binding Assay. A direct binding assay that measures radiolabeled inhibitor binding to enzyme was developed to assess binding directly to COX-1 and COX-2 without the confounding influence of enzyme ...
Materials. Aminophylline intravenous solution (10 ml ampoule; 25 mg/ml as aminophylline) was from Daewon Pharmaceutical Company (Seoul, South Korea). 1,3-DMU, β-hydroxyethyltheophylline [internal standard for the high-performance liquid chromatographic (HPLC) analysis of theophylline and 1,3-DMU], KPLPS (purified by phenol extraction; protein ,3%), β-actin, primary monoclonal antibody for β-actin, EDTA (as a disodium salt), and Kodak X-OMAT film were purchased from Sigma-Aldrich (St. Louis, MO). Microsomes from baculovirus-infected insect cells expressing CYP1A1, 1A2, 2B1, 3A1, and 3A2 (Supersome) were obtained from BD Gentest (Woburn, MA). Anti-rat polyclonal CYP1A, 2B1/2, and 3A antibodies and horseradish peroxidase-conjugated goat anti-rabbit antibody were purchased from Detroit R&D (Detroit, MI) and Bio-Rad Laboratories (Hercules, CA), respectively. Enhanced chemiluminescence reagents were products from Amersham Biosciences Corporation (Piscataway, NJ). Other chemicals were of reagent or ...
Characterization of two human cAMP-specific phosphodiesterase subtypes expressed in baculovirus-infected insect cells. (pages 477-484). Bernard Y. Amegadzie, Charles R. Hanning, Megan M. McLaughlin, Miriam Burman, Lenora B. Cieslinski, George P. Livi and Theodore J. Torphy. Version of Record online: 2 JAN 2013 , DOI: 10.1006/cbir.1995.1091. ...
Development of a stable expression vector system for gene transfer into sceletal muscle followinq detection of sequence instability in utrophin cDNA during cloning ...
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... ,Recognizes the 41 and 43 kDa G-proteins present in liver plasma membranes and Gq/11alpha expressed in the Sf9 insect cell expression system. Negative for recombinant G(I)alpha subtypes.,biological,biology supply,biology supplies,biology product
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pOET2C 6xHis is a baculovirus transfer vector under control of the AcMNPV polyhedron (polh) promoter with additional C-terminal 6xHis-Tag fusion sequence
Figure 1 Antiphospho-Pak1 antibody specifically recognizes activated Pak1. (A) Activation loop sequence of the phosphorylated peptide used for production of polyclonal antiphospho-Pak1 antisera. (B) Immunoblots (lanes 1-4) and radiolabeled kinase reaction products (lanes 5 and 6) of baculovirus-produced Pak1. Purified KD or CA Pak1 was incubated in protein kinase buffer in the presence of ATP, then immunoblotted using anti-Pak1 (lanes 1 and 2) and antiphospho-Pak antisera (lanes 3 and 4) autoradiographed to detect antibody-bound proteins. Although both forms of the protein were detected with anti-Pak1, only activated Pak1 was detected using the phospho-specific antibody, despite the large amount of recombinant protein used in this assay. Lanes 5 and 6 show autoradiographed PAGE gel of reaction products from a kinase assay performed in the presence of [γ-32P]ATP. Molecular mass in kilodaltons is shown to the right of the blot. (C) Comparison between immunoblot and radiolabeled kinase reaction ...
Rho-kinase II (ROCK-II) is a serine/threonine kinase that is involved in regulation of smooth muscle contraction and has been shown to contribute to the early stages of axon formation in neurons and the regulation of the neuronal cytoskeleton. Much of what is known about Rho-kinase function comes from cell-biological studies, whereas a paucity of biochemical characterization exists for the enzyme. In an effort to characterize ROCK-II biochemically we have cloned a truncated form of human ROCK-II comprising amino acids 1-543 and overexpressed it in Sf-21 cells. Utilizing the Sf-21/baculovirus expression system we isolated milligram quantities of ROCK-II (1-543) and purified the enzyme to near homogeneity. Optimal expression conditions revealed that infection of Sf-21 cells at a multiplicity of infection of 10 for 72h yielded maximal protein expression. Expression of ROCK-II (1-543) as an N-terminal Flag fusion protein allowed a single-step purification yielding greater than 90% homogeneous ...
Avian H7N9 influenza virus infection with fatal outcomes continues to pose a pandemic threat and highly immunogenic vaccines are urgently needed. In this report we show that baculovirus-derived recombinant H7 hemagglutinin protein, when delivered with RIG-I ligand, induced enhanced antibody and T cell responses and conferred protection against lethal challenge with a homologous H7N9 virus. These findings indicate the potential utility of RIG-I ligands as vaccine adjuvants to increase the immunogenicity of recombinant H7 hemagglutinin ...
Insect cell culture is widely used as an industrial platform in recombinant protein production for research and diagnostics (1, 3,4,5). Many commercial yeastolate products are intended as nutritional supplements for not only insect cell culture, but also for mammalian cell culture and bacterial fermentation applications (6,7,8,9,10,11,12,13,14,15). Yeastolate plays an important role in modern biological production because it can be very effective in promoting insect cell growth and enhancing production of recombinant proteins. The product is known to contain amino acids,…. ...
BioAssay record AID 445124 submitted by ChEMBL: Inhibition of human recombinant HDAC1 expressed in baculovirus assessed as residual activity at 5 uM.
Recombinant fragment corresponding to amino acids 142-481 of Human AKT2 with N-terminal His tag expressed in a Baculovirus infected Sf9 cell expression system; 340 amino acids, MW 41kDa.
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Buy our Recombinant human MAP3K9 protein. Ab125554 is an active protein fragment produced in Baculovirus infected Sf9 cells and has been validated in WB…
Buy our Recombinant human FGFR3 protein. Ab60857 is an active protein fragment produced in Baculovirus infected Sf9 cells and has been validated in FuncS…
Recombinant SARS Coronavirus spike Protein (Met1-Arg667) S1 Subunit His Tag 40150-V08B1 with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this SARS Coronavirus spike protein for relevant bioassay and related research.
Recombinant SARS Coronavirus spike Protein (Arg306-Phe527) RBD His Tag with a fusion His Tag, is expressed in Baculovirus-Insect Cells. With high purity, high biological activity, high stability, and other superior features, you can use this SARS Coronavirus spike protein for relevant bioassay and related research.
The DNA polymerase genes of human cytomegalovirus (HCMV) and varicella-zoster virus (VZV) were inserted separately into the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV) by cotransfection of Spodoptera frugiperda (SF9) cells with baculovirus transfer vectors carrying the genes and AcNPV infectious DNA. Infection of SF9 cells with the recombinant viruses resulted in expression from the polyhedrin promoter of proteins of the expected Mrs. These proteins possessed DNA polymerase activities similar to that of the enzymes induced by the respective herpesvirus in infected cells, and were identified as HCMV and VZV DNA polymerase using inhibitors and specific antisera reactive with each enzyme.
Targeting of viral vectors to specific cells has received an increasing interest in the area of therapeutic gene transfer. However, specific targeting to tumors, especially to tumor vasculature has been impeded by the limitations of present vector systems and lack of selective markers. Therefore, peptides that home to specific sites in the tumor vasculature or tumors, are attractive as targeting moieties for gene therapy purposes. Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a prototype of the Baculoviridae family, is a promising new vector candidate for gene therapy. Moreover, baculovirus display -technology enables the presentation of targeting moieties on the viral surface. The goal of this study was to develop baculovirus vectors for targeted and enhanced gene transfer to cancer cells. To this end, specific tumor and tumor vasculature targeting peptides, such as RGD, RKK, LyP-1, F3 and CGKRK, were successfully displayed on the baculoviral surface. Most importantly, the ...
A baculovirus-expressed chimeric recombinant IgG1 (rIgG1) antibody, with Cgamma1 and Ckappa human constant domains, was derived from the murine monoclonal antibody (mAb) 13B8.2, which is specific for the CDR3-like loop of the CD4 molecule and which inhibits HIV-1 replication. Chimeric rIgG1 antibody 13B8.2 blocked, in a dose-dependent manner, antigen presentation through inhibition of subsequent IL-2 secretion by stimulated T cells. The one-way mixed lymphocyte reaction was abrogated by previous addition of baculovirus-produced rIgG1 13B8.2 in the T-cell culture. Anti-proliferative activity of rIgG1 was demonstrated on CD3-activated CD4+ T lymphocytes from healthy donors, such effect being associated with reduced IL-2 secretion of activated T cells. On the other hand, no proliferation inhibition was observed on CD4+ T lymphocytes activated with phorbol ester plus ionomycin, suggesting that rIgG1 13B8.2 preferentially acts on a proximal TCR-induced signaling pathway. Treatment of DBA1/J human CD4
Looking for online definition of baculovirus in the Medical Dictionary? baculovirus explanation free. What is baculovirus? Meaning of baculovirus medical term. What does baculovirus mean?
Progesterone receptor ligand binding domain (PR-LBD) is a 59.83 kDa recombinant human protein (amino acids 675-933) expressed as a glutathione-S-transferase (GST) fusion protein in baculovirus-infected insect cells. 600UG Progesterone Receptor Recombinant HumanProtein, Ligand Binding Domain ...
Chemicals.d-Glucose-6-phosphate, glucose-6-phosphate dehydrogenase, NADP+, sulfaphenazole, quinidine, clomethiazole, furafylline, 8-methoxypsoralen, 3′-p-hydroxypaclitaxel, and paclitaxel were purchased from Sigma-Aldrich (St. Louis, MO). Ketoconazole was obtained from MP Biomedicals (Irvine, CA). S-Mephenytoin was purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). 6α-Hydroxypaclitaxel was obtained from BD Gentest (Woburn, MA). Quercetin was purchased from Acros Organics (Geel, Belgium). Cephalomannine (96%) was purchased from Shanghai Jinhe Bio-Technology Co. (Shanghai, China). All other reagents were of HPLC-grade or of the highest grade commercially available. cDNA-expressed recombinant CYP1A2, CYP2A6, CYP2C9, CYP2D6, CYP2E1, and CYP3A4 derived from baculovirus-infected insect cells coexpressing NADPH-P450 reductase were obtained from BD Gentest. cDNA-expressed CYP2C8 and CYP2C19 in Escherichia coli coexpressing NADPH-P450 reductase were purchased from New England ...
Effect of Individual and Combined Treatment with Azadirachtin and Spodoptera littoralis Multicapsid Nucleopolyhedrovirus (SpliMNPV, Baculoviridae) on the Egyptian Cotton Leafworm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) ...
Both humoral and cellular immune responses are critical for the control of HIV infection and replication. We have established systems for production of HIV and SIV virus-like particles containing high levels of viral Env proteins using the baculovirus expression system. Evaluation of immunogenicity showed that immunization with virus-like particles induced both cellular and neutralizing antibody responses. Furthermore, mucosal administration of virus-like particles effectively induced both mucosal and systemic immune responses. These results indicate that virus-like particles consisting of HIV structural proteins are an attractive vaccine platform for eliciting anti-viral immune responses, especially neutralizing antibody responses. We have also synthesized codon-optimized genes for HIV Env proteins and evaluated their immunogenicity. Combinations of virus-like particle and DNA-based vaccination are promising for inducing strong cellular and neutralizing antibody responses against HIV ...
Hello - Does anybody can help with working protocol about differential or gradient centrifugation for subcellular fractionation of insect cells (Sf9 cell line)? Also, any information about the densities or/and sedimentation coefficients of different types of INSECT cell organelles would be helpful. Unfortunately, I have no possibility for electron microscopy and no knowledge about marker enzymes for insect cell organelles to design a good experimental protocol for determining the insect cell subcellular fractions. Merike Meier, PhD student meerike at kbfi.ee ...
Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa ...
Human ALK (BAG10812.1, 1058 a.a. - 1620 a.a.) C1156Y mutant partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells. (P5736) - Products - Abnova
Human NEK1 (NP_036356.1, 1 a.a. - 505 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. (P5609) - Products - Abnova
ID AC83_NPVAC Reviewed; 847 AA. AC Q06670; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 31-JAN-2018, entry version 76. DE RecName: Full=Capsid-associated protein AC83; DE Flags: Precursor; GN Name=p95; ORFNames=ORF83; OS Autographa californica nuclear polyhedrosis virus (AcMNPV). OC Viruses; dsDNA viruses, no RNA stage; Baculoviridae; Alphabaculovirus. OX NCBI_TaxID=46015; OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C6; RX PubMed=8030224; DOI=10.1006/viro.1994.1380; RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.; RT "The complete DNA sequence of Autographa californica nuclear RT polyhedrosis virus."; RL Virology 202:586-605(1994). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=E2; RX PubMed=8126447; DOI=10.1099/0022-1317-75-3-487; RA Kool M., Broer R., Zuidema D., Goldbach R.W., Vlak J.M.; RT "Nucleotide sequence and genetic organization of a ...
Insect pathogenic viruses can be divided into five groups. Nuclear polyhedrosis viruses, cytoplasmic polyhedrosis viruses, granulosis viruses, and pox-like viruses are viruses of the occluded type in which the virus particles are embedded in a large crystalline inclusion which may be either polyhedral or capsular in shape. Other viruses do not occur in such inclusion-bodies and are often referred to as non-occluded viruses. Nuclear polyhedrosis viruses have been extensively investigated but knowledge of many of their properties is ill-defined or lacking and assumptions have been made about their structure and multiplication which are contrary to accepted concepts of virus assembly. In this work two nuclear polyhedrosis viruses have been investigated, largely by electron microscopic methods, in order to determine how the structural and dynamic properties of the viruses can be correlated. Consideration has also been given to the compliance of these properties with current thinking on virus ...
A laboratory culture of Spodoptera exigua was examined to assess covert (latent or persistent) baculovirus infections and spontaneous disease outbreaks. Two nucleopolyhedrovirus (NPV) species were found to be reactivated from a covert state in a laboratory culture of S. exigua to fully lethal forms. These were identified as S. exigua multinucleopolyhedrovirus (SeMNPV) and Mamestra brassicae NPV (MbNPV) using restriction enzyme analysis of purified viral DNA. Sequence data derived from both overtly and covertly virus-infected insects revealed highly conserved sequences for lef-8, lef-9 and polyhedrin gene sequence (98-100 % nucleotide identity to SeMNPV published sequence). By monitoring spontaneous overt infections and quantifying viral DNA (by quantitative-PCR) in asymptomatic individuals over two generations we identified fluctuating trends in viral DNA levels from covert SeMNPV and MbNPV within an S. exigua host population. Virus levels per insect life stage ranged from 3.51±0.101×105 to ...
TY - JOUR. T1 - Conformation and Glycosylation of A Megalin Fragment Correlate with Nephritogenicity in Heymann Nephritis. AU - Tramontano, Alfonso. AU - Makker, Sudesh P. PY - 2004/2/15. Y1 - 2004/2/15. N2 - Active Heymann nephritis (AHN), a rat model of autoimmune glomerulonephritis, is induced by immunization with autologous megalin, a 600-kDa cell surface glycoprotein isolated from crude renal extracts. Recombinant proteins containing a 563-residue N-terminal sequence of megalin were obtained from Escherichia coli and baculovirus-insect cell expression systems. Rats immunized with the soluble, secreted protein encoded by a baculovirus construct elicited high titer anti-megalin autoantibodies and developed glomerular immune deposits and elevated proteinuria consistent with AHN. Rats treated with the bacterial or nonsecreted insect cell proteins produced a milder anti-megalin response and did not develop the disease. Nephritogenicity appeared to correlate with conformational or other ...
Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75% formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the...
Chikungunya fever is a pandemic disease caused by the mosquito-borne Chikungunya virus (CHIKV). E1 glycoprotein mediation of viral membrane fusion during CHIKV infection is a crucial step in the release of viral genome into the host cytoplasm for replication. How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored. A bicistronic baculovirus expression system to produce recombinant baculoviruses for cell-based assay was used. Sf21 insect cells infected by recombinant baculoviruses bearing wild type or single-amino-acid substitution of CHIKV E1 and EGFP (enhanced green fluorescence protein) were employed to investigate the roles of four E1 amino acid residues (G91, V178, A226, and H230) in membrane fusion activity. Western blot analysis revealed that the E1 expression level and surface features in wild type and mutant substituted cells were similar. However, cell fusion assay found that those cells infected by
Chikungunya fever is a pandemic disease caused by the mosquito-borne Chikungunya virus (CHIKV). E1 glycoprotein mediation of viral membrane fusion during CHIKV infection is a crucial step in the release of viral genome into the host cytoplasm for replication. How the E1 structure determines membrane fusion and whether other CHIKV structural proteins participate in E1 fusion activity remain largely unexplored. A bicistronic baculovirus expression system to produce recombinant baculoviruses for cell-based assay was used. Sf21 insect cells infected by recombinant baculoviruses bearing wild type or single-amino-acid substitution of CHIKV E1 and EGFP (enhanced green fluorescence protein) were employed to investigate the roles of four E1 amino acid residues (G91, V178, A226, and H230) in membrane fusion activity. Western blot analysis revealed that the E1 expression level and surface features in wild type and mutant substituted cells were similar. However, cell fusion assay found that those cells infected by
Ethical Considerations about sildenafil 100mg preisvergleich EHR-Mediated Results Disclosure and Pathology Information Presented via Patient Portals. Together, these results render the F3 peptide a tool for elucidating the mechanism and molecular details conferring to baculovirus-mediated gene transduction in mammalian cells. Neurologic manifestations are common in patients with antiphospholipid antibodies and include stroke, seizures, dementia, cognitive dysfunction, chorea, migraine, psychosis, and demyelinating disease.. Changes in catecholamine metabolism in the rostral ventrolateral medulla following halothane and nitroprusside-induced hypotension: an in vivo electrochemical study. Moreover, our sildenafil 100 mg study provided potential application value for improving fruit set. FX-58, which was separated from a marine plant collected in Qingdao.. ECD is a rare form of non-Langerhans histiocytosis that affects multiple organs. Surface-enhanced Raman scattering of single-walled sildenafil ...
Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be infected with polyhedra by droplet feeding and diet plug-based bioassays. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents, including discussion of the pros and cons for use of baculoviruses as insecticides, and progress made in genetic enhancement of baculoviruses for improved insecticidal efficacy.
Looking for online definition of BEVS or what BEVS stands for? BEVS is listed in the Worlds largest and most authoritative dictionary database of abbreviations and acronyms
Advances in the Application of Perfusion Technologies to Drosophila S2 Insect Cell Culture" Lars Poulsen and Willem A. de Jongh, pp 165-182. Continuous Processing in Pharmaceutical Manufacturing, First Edition. Edited by Ganapathy Subramanian. December 2014 Wiley VCH. ISBN: 978-3-527-33595-4. Read more. ...
Programmed cell death is an active process of self destruction that is important in both the development and maintenance of multicellular animals. The molecular mechanisms controlling activation or suppression of programmed cell death are largely unknown. Apoptosis, a morphologically and biochemically defined type of programmed cell death commonly seen in vertebrates, was found to be initiated during baculovirus replication in insect cells. A specific viral gene product, p35, was identified as being responsible for blocking the apoptotic response. Identification of the function of this gene will allow further definition of the molecular pathways involved in the regulation of programmed cell death and may identify the role of apoptosis in invertebrate viral defense systems. ...
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FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Recombinant human MAST2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Species :Human Tag :GST tag Expression System:Sf9 insect cells using baculovirus Sequence :1-792 Genbank Number :BC015816 Purity :Sample Purity Data. For s
Our BacMam Histone H3 Cellular Assay Kits allow you to choose the modification that corresponds to your enzyme of interest in your chosen cell background or multiple cell backgrounds. Our BacMam Histone H3 Cellular Assay Kits allow you to choose the modification that corresponds to your enzyme of interest in your chosen cell background or multiple cell backgrounds.
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Nagao, M. and Masuda, S. and Ueda, M. and Sasaki, R. (1996) Characterisation and analysis of human interferon-gamma glycoforms produced in Baculovirus infected Spodoptera frugiperda (Sp9) and Estigena acrea (EA) cell lines. In: Beuvery, E.C. and Griffiths, J.B. and Zeijlemaker, W.P., eds. Animal Cell Technology: Developments Towards the 21st Century. Kluwer Academic Publishers, Netherlands, pp. 427-430. ISBN 0-7923-3736-0. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided ...
HTRA2 / PRSS25, 50 µg. Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication.
Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time…
Spodoptera frugiperda SF-21 cells infected with Autographa californica nuclear polyhedrosis virus mutants which lack a functional p35 gene undergo apoptosis, a type of programmed cell death. To identify p35-homologous genes in other baculoviruses, A. californica nuclear polyhedrosis virus DNA containing a deletion in p35 was cotransfected into SF-21 cells along with genomic DNAs from other baculoviruses. One of the viral DNAs which were able to rescue wild-type infection was from Cydia pomonella granulosis virus (CpGV). The CpGV gene responsible for the effect was mapped to a 1.6-kb SalI-SstI subclone of the SalI B fragment of CpGV. The sequence of the SalI-SstI subclone revealed an open reading frame capable of encoding a polypeptide of 31 kDa which was sufficient to rescue wild-type infection; this gene was thus called iap (inhibitor of apoptosis). The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found ...
Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approximately 2.7 nm. By comparing capsids assembled with and without the expression of gene UL35, we have confirmed the presence of six copies of its product, VP26 (12 kDa), around each hexon tip. However, VP26 is not present on pentons, indicating that the conformational differences between the hexon and penton states of the major capsid protein, VP5, extend to the VP26 binding site. ...
The nitric oxide synthases are dimeric enzymes in which the intersubunit contacts are formed by the P-450-haem-containing, tetrahydrobiopterin-dependent oxygenase domain. The dimerization of the neuronal isoenzyme was shown previously to be triggered by Fe-protoporphyrin IX (haemin). We report for the first time the reactivation of the haem-deficient neuronal isoenzyme (from rat, expressed in a baculovirus/insect cell system) after haem reconstitution. We further examined the reconstitution of the enzyme with protoporphyrin IX (PPIX) and its Mn and Co complexes. All of these porphyrins inserted into the haem pocket, as assessed by quenching of intrinsic protein fluorescence. In addition to haemin, MnPPIX stimulated dimerization, as measured by gel filtration and by cross-linking with glutaraldehyde. In contrast, neither CoPPIX nor PPIX stimulated dimerization. The absorbance spectra of the reconstituted enzymes were measured and compared with published results on P-450 enzymes reconstituted with ...
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This enabled the expression of a foreign gene in addition to the virus polyhedrin. , 1988). Each foreign sequence was placed under the control of the native or duplicated polyhedrin gene promoter. Similar expression vectors were derived by using a combination of the polyhedrin and p10 gene promoters (Weyer and Possee, 1991). A copy of the p10 gene promoter was inserted upstream of the polyhedrin gene promoter. The influenza virus haernagglutinin o r neuraminidase gene was placed under the control of each promoter and co-synthesis achieved in recombinant virus-infected cells. The influenza virus haernagglutinin o r neuraminidase gene was placed under the control of each promoter and co-synthesis achieved in recombinant virus-infected cells. Baculovirus expression vectors are not limited to the production of two foreign proteins in insect cells. , 1990). Five bluetongue virus structural proteins have been co-expressed within the same cell by coinfection of two dual recombinants and one single ...
Norwalk virus NV and Norwalk-like viruses are important causes of epidemic nonbacterial gastroenteritis in older children and adults. Serologic responses to NV of 154 Finnish infants and young children participating in a rotavirus vaccine study were examined by ELISA with a recently available baculovirus-expressed recombinant NV capsid protein....
Saliki, J.T.; Mizak, B.; Flore, H.P.; Gettig, R.R.; Burand, J.P.; Carmichael, L.E.; Wood, H.A.; Parrish, C.R., 1992: Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs
The recombinant mature AcSecapin-1 peptide was expressed in baculovirus-infected insect cells. AcSecapin-1 functions as a serine protease inhibitor-like peptide that has inhibitory effects against plasmin, elastases, microbial serine proteases, trypsin, and chymotrypsin. Consistent with these functions, AcSecapin-1 inhibited the plasmin-mediated degradation of fibrin to fibrin degradation products, thus indicating the role of AcSecapin-1 as an anti-fibrinolytic agent. AcSecapin-1 also inhibited both human neutrophil and porcine pancreatic elastases. Furthermore, AcSecapin-1 bound to bacterial and fungal surfaces and exhibited anti-microbial activity against fungi and gram-positive and gram-negative bacteria ...
Antibodies in porcine sera against glycoprotein E (gE) of pseudorabies virus (PRV) are usually measured in blocking enzyme-linked immunosorbent assays (ELISAs) with one or two murine monoclonal antibodies (MAbs) directed against gE. Our aim was to develop a confirmation assay which is based on another principle and which is able to detect antibodies directed against most potential binding sites on gE with high specificity. Therefore, we developed an indirect double-antibody sandwich assay (IDAS) using recombinant gE expressed by baculovirus (BacgE960). A fragment of the gE gene consisting of nucleotide positions +60 to +1020 of gE, coding for the major antigenic sites of gE but not the transmembrane region, was cloned behind the signal sequence of PRV gG and the p10 promoter in a baculovirus vector. Immunoblot analysis showed that the expressed protein reacted with MAbs directed against five of the six antigenic sites on gE. Although the conformation of some antigenic sites, notably antigenic ...
Background :The presence of anti-parvovirus B19 (B19V) IgM against viral capsid proteins (VP1 and VP2) has long been used to detect recent infection. The utility of antibodies directed against B19V NS1 protein has received less attention as a serological indicator of recent infection, although anti-B19V NS1 IgG has been associated with persistent infection. Objecties : To elucidate the role of anti-B19V NS1 antibody detection in recent infection, full-length B19V NS1 was expressed and purified. The resultant antigen was used to develop both Western blot assays and microplate ELISA for the detection of NS1 antibodies. Study design : Serum specimens were obtained from individuals recently infected with B19V (children (n=16), adults (n=40)) and from 17 individuals with no evidence of recent B19V infection. All specimens were screened for anti-B19V NS1 IgG and IgM. Results : It was observed that 68.8% (11/16) of children recently infected with B19V were anti-B19V NS1 IgG seropositive. Furthermore, ...
ESF 921 Delta Series Methionine Deficient insect cell culture media is based on the popular ESF 921™ medium which provides excellent growth for a wide variety of insect cells. The methionine deficient media allows the addition of methionine amino acids to target proteins for observing protein structure and dynamics.
Familie Baculoviridae. *Genus Alphabaculovirus. *Genus Betabaculovirus. *Genus Gammabaculovirus. *Genus Deltabaculovirus. * ...
There are two genera in the Baculoviridae: Nucleopolyhedrovirus and Granulovirus. Of these, a granulovirus species specific to ...
Famili Baculoviridae. *Famili Coccolithoviridae. *Famili Corticoviridae. *Famili Fuselloviridae. *Famili Guttaviridae. *Famili ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
They are members of the Baculoviridae, a family that is restricted to insects. Most baculovirus isolates have been obtained ...
... is a genus of viruses, in the family Baculoviridae. Hymenoptera serve as natural hosts. There are currently ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus ...
... is a genus of viruses, in the family Baculoviridae. Mosquito larvae serve as natural hosts. There is currently ... Baculoviridae Genus: Deltabaculovirus Culex nigripalpus nucleopolyhedrovirus Viruses in Deltabaculovirus are enveloped. Genomes ...
... is a genus of viruses, in the family Baculoviridae. Winged insects, arthropods, lepidoptora, hymenoptera, ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ...
... is a genus of viruses, in the family Baculoviridae. Arthropods serve as natural hosts. There are currently 17 ... Group: dsDNA Order: Unassigned Family: Baculoviridae Genus: Betabaculovirus Adoxophyes orana granulovirus Agrotis segetum ...
... (CpGV) is a granulovirus belonging to the family Baculoviridae. It has a double-stranded DNA ...
From the family Baculoviridae, it is a type of Alphabaculovirus and its genome is 80-180kb long. NPVs are commonly used as ...
Viruses from the family Baculoviridae induce in their hosts changes to both feeding behavior and environment selection. They ...
Some have circular genomes (Baculoviridae, Papovaviridae and Polydnaviridae) while others have linear genomes (Adenoviridae, ... Baculoviridae), fish herpesvirus (Herpesviridae), T-even bacteriophages (Myoviridae) and poxviruses (Poxviridae) were not ... Baculoviridae, Fuselloviridae, Globuloviridae, Guttaviridae, Hytrosaviridae, Iridoviridae, Lipothrixviridae, Nimaviridae and ... infection Family Ampullaviridae Family Ascoviridae Family Asfarviridae-includes African swine fever virus Family Baculoviridae ...
The NPVs come under the family of baculoviridae and its virions are enveloped rod shaped nucleocapsids containing circular, ...
Since baculoviridae infect only insects and not humans, the function of P35 in the immune evasion of infected cells is not ...
Both in America and in Europe, research continues into biological control of the species, and for example the Baculoviridae ...
Baculoviridae), respectively. Only one genus in this family is currently recognised: Bidensovirus. Virus Taxonomy: Ninth Report ...
Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ipsilon multiple nucleopolyhedrovirus ...
Adenoviridae Asfarviridae Baculoviridae Herpesviridae Iridoviridae The VBRC database stores viral bioinformatic data on three ...
Superkingdom Viruses No classification - dsDNA viruses, no RNA stage Family - Baculoviridae Genus - Alphabaculovirus Species - ...
... but the term now refers to 35 species of the genus Baculoviridae-mostly alphabaculoviruses, but also one deltabaculovirus and ...
Baculoviridae Barnaviridae Benyviridae Betaflexiviridae Betaherpesvirinae Bicaudaviridae Bidnaviridae Birnaviridae Bornaviridae ...
ISBN 0-306-45641-9. Viralzone: Baculoviridae ICTV Index of Viruses - Baculoviridae (2006). In: ICTVdB - The Universal Virus ... Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, diptera, and decapoda serve as natural hosts. There ... Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ipsilon multiple nucleopolyhedrovirus ...
When retroviruses have integrated their own genome into the germ line, their genome is passed on to a following generation. These endogenous retroviruses (ERVs), contrasted with exogenous ones, now make up 5-8% of the human genome.[7] Most insertions have no known function and are often referred to as "junk DNA". However, many endogenous retroviruses play important roles in host biology, such as control of gene transcription, cell fusion during placental development in the course of the germination of an embryo, and resistance to exogenous retroviral infection. Endogenous retroviruses have also received special attention in the research of immunology-related pathologies, such as autoimmune diseases like multiple sclerosis, although endogenous retroviruses have not yet been proven to play any causal role in this class of disease.[8] While transcription was classically thought to occur only from DNA to RNA, reverse transcriptase transcribes RNA into DNA. The term "retro" in retrovirus refers to ...
unclassified Baculoviridae Urbanus proteus nucleopolyhedrovirus NC_029997. complete. isolate:Southern Brazil. -. 105555 nt. 119 ...
Baculoviridae. From MicrobeWiki, the student-edited microbiology resource. Revision as of 18:57, 26 June 2006 by Mschlemm. ( ... The two genera in the family Baculoviridae are definied by their different OV structure. Granulovirus OVs contain only one ... There is some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. The reasons for ... Retrieved from "https://microbewiki.kenyon.edu/index.php?title=Baculoviridae&oldid=4492" ...
ISBN 0-306-45641-9. Viralzone: Baculoviridae ICTV Index of Viruses - Baculoviridae (2006). In: ICTVdB - The Universal Virus ... Baculoviridae is a family of viruses. Arthropods, lepidoptera, hymenoptera, diptera, and decapoda serve as natural hosts. There ... Baculoviridae Genus: Alphabaculovirus Adoxophyes honmai nucleopolyhedrovirus Agrotis ipsilon multiple nucleopolyhedrovirus ...
Baculoviridae. Chapter contents. Posted June 2018. Baculoviridae: The family. *Citation, Summary, Virion, Genome, Antigenicity ... Figure 3.Baculoviridae. Phylogeny of family Baculoviridae. The maximum likelihood tree, based on the alignment of 38 core gene ... Nucleocapsids contain one molecule of circular, supercoiled dsDNA of 80-180 kbp (Figure 1.Baculoviridae, Figure 2.Baculoviridae ... The Baculoviridae is a family of large, insect-specific viruses with circular dsDNA genomes ranging from 80 to 180 kbp. Virions ...
This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Baculoviridae, ... which is available at www.ictv.global/report/baculoviridae. ... The family Baculoviridae comprises large viruses with circular ... 4. Tanada Y, Hess RT. Baculoviridae. In Adams JR, Bonami JR. (editors) Atlas of Invertebrate Viruses Boca Raton, FL: CRC Press ... ICTV Virus Taxonomy Profile: Baculoviridae, Page 1 of 1 , Previous page , Next page , /docserver/preview/fulltext/jgv/99/9/1185 ...
The nucleocapsids are enveloped singly, and multiple virions are assembled into each OB (Figure 1.Gammabaculovirus.). The virus is restricted to the host midgut and causes what was previously described in the literature as "infectious diarrhea". Genome sequencing analyses of three viruses - Neodiprion lecontei nucleopolyhedrovirus (NeleNPV), Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), and Neodiprion abietis nucleopolyhedrovirus (NeabNPV) - revealed that these viruses do not encode typical envelope fusion proteins found in other baculoviruses (Arif et al., 2011). This observation has raised the question of whether the budded virus phenotype plays a role in gammabaculovirus biology.. ...
Baculoviridae - Herniou, E., Theilmann, D.A., Blissard, G.W., Becnel, J.J., Basil, A., Harrison, R.L., Bonning, B.C., Vlak, J.M ... Baculoviridae. Virus Taxonomy: Classification and Nomenclature of Viruses. oxford:Elsevier 163-174. ...
The present invention relates to detecting target nucleic acid sequences in pooled samples. In particular, the present invention relates to compositions and methods for detecting the presence or absence of target nucleic acid sequences (e.g. RNA virus sequences) in a pooled sample employing an INVADER detection assay. In certain embodiments, the present invention allows target nucleic acid sequence detection in pooled biological samples (e.g. pooled blood samples) without prior amplification of the target.
Familie Baculoviridae. *Genus Alphabaculovirus. *Genus Betabaculovirus. *Genus Gammabaculovirus. *Genus Deltabaculovirus. * ...
Baculoviridae, Filoviridae, Flaviviridae, Arenaviridae, Rhabdoviridae, Paramyxoviridae, and Coronaviridae [19, 20] (Table 3.1 ...
Baculoviridae. Genus. Alphabaculovirus. Genus. Betabaculovirus. Genus. Gammabaculovirus. Genus. Deltabaculovirus. Family. ...
There are two genera in the Baculoviridae: Nucleopolyhedrovirus and Granulovirus. Of these, a granulovirus species specific to ...
Baculoviridae:. Nucleopolyhedrovirus. Autographa californica nucleopolyhedrovirus. Invertebrates. Granulovirus. Plodia ...
Famili Baculoviridae. *Famili Coccolithoviridae. *Famili Corticoviridae. *Famili Fuselloviridae. *Famili Guttaviridae. *Famili ...
Baculoviridae (e.g. insect baculovirus). *Hepadnaviridae (e.g. Hepatitis B). *Herpesviridae (e.g. herpesviruses, chickenpox ( ...
Baculoviridae; Alphabaculovirus. OX NCBI_TaxID=46015; OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths). RN [1] RP ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
棒状病毒科 Baculoviridae. *Bicaudaviridae(英语:Bicaudaviridae). *Clavaviridae(英语:Clavaviridae) ...
Baculoviridae / genetics* * Base Sequence * Cloning, Molecular * DNA Primers / chemistry * Drosophila Proteins* * Drosophila ...
Baculoviridae: Nuclear Polyhedrosis Viruses. Part I. Nuclear Polyhedrosis Viruses of Insects. In Atlas of Invertebrate Viruses ...
They are members of the Baculoviridae, a family that is restricted to insects. Most baculovirus isolates have been obtained ...
Baculoviridae. Badnavirus. Bicaudaviridae. Birnaviridae. Bromoviridae. Bromovirus. Bunyaviridae. Caulimoviridae. Caulimovirus. ...
3). For example, viruses belonging to the family Baculoviridae branched from the Asfarviridae outgroup with a likelihood ... Baculoviridae, and Herpesviridae. Several sequences from DGGE (SO98-1, SO98-2, SO98-3, BSA99-2, BSA99-5, SIA99-1, MIB99-2, and ... Baculoviridae; H, Herpesviridae; P, Phycodnaviridae. The Phycodnaviridae are also indicated in boldface lettering. The scale ... viruses from the families Baculoviridae and Herpesviridae all clustered with viruses of the same family. ...
2005 Family Baculoviridae. In Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses (eds Fauquet ...
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  • The Baculoviridae family can be divided into four genera according to common biological and structural characteristics: Alphabaculovirus, which includes lepidopteran specific baculoviruses and is subdivided into Group I or Group II based on the type of fusogenic protein, Betabaculovirus, comprising lepidopteran-specific granuloviruses, Gammabaculovirus, which includes hymenopteran-specific baculoviruses, and Deltabaculovirus, which, to date, comprises only CuniNPV and possibly still undescribed dipteran-specific baculoviruses [ 3 - 6 ]. (omicsonline.org)
  • Vuill, a granulosis virus (Baculoviridae: Eubaculovirinae), and a protozoan (phylum Microspora) were found. (bioone.org)
  • La persistencia del virus es alta en el suelo de todos los cultivos aunque su incidencia es mayor (mas) en primavera y verano. (worldwidescience.org)
  • Se han podido identificar hasta nueve variantes genotípicas del virus en esta zona. (worldwidescience.org)
  • Compuestos derivados del estilbeno tienen una actividad sinérgica con los OBs en condiciones de laboratorio y reducen la dosis letal del virus en campo. (worldwidescience.org)
  • Actualmente el virus se produce masivamente en una planta comercial y se ha iniciado el proceso de registro del producto para su utilización en cultivos de pimiento dulce en los invernaderos de Almería. (worldwidescience.org)
  • Baculoviridae ) are generated when nucleocapsids bud through the plasma membrane at the surface of infected cells. (ictvonline.org)