Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. The type species is Autographa californica nucleopolyhedrovirus.
A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.

Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. (1/2544)

To understand the initiation of the transcription of protein-coding genes, we have dissected the role of the basal transcription/DNA repair factor TFIIH. Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides, we were able to analyze the role of XPB, XPD, and cdk7 subunits in the transcription reaction. Designing mutated recombinant subunits, we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase, which is dispensable, stimulates transcription and allows the CAK complex to be anchored to TFIIH. In addition, we also show that cdk7 may phosphorylate the carboxy-terminal domain (CTD) of RNA pol II in the absence of promoter opening.  (+info)

A plant 126-kDa phosphatidylinositol 4-kinase with a novel repeat structure. Cloning and functional expression in baculovirus-infected insect cells. (2/2544)

Phosphatidylinositol metabolism plays a central role in signaling pathways in animals and is also believed to be of importance in signal transduction in higher plants. We report here the molecular cloning of a cDNA encoding a previously unidentified 126-kDa phosphatidylinositol (PI) 4-kinase (AtPI4Kbeta) from the higher plant Arabidopsis thaliana. The novel protein possesses the conserved domains present in animal and yeast PI 4-kinases, namely a lipid kinase unique domain and a catalytic domain. An additional domain, approximately 300 amino acids long, containing a high percentage (46%) of charged amino acids is specific to this plant enzyme. Recombinant AtPI4Kbeta expressed in baculovirus-infected insect (Spodoptera frugiperda) cells phosphorylated phosphatidylinositol exclusively at the D4 position of the inositol ring. Recombinant protein was maximally activated by 0.6% Triton X-100 but was inhibited by adenosine with an IC50 of approximately 200 microM. Wortmannin at a concentration of 10 microM inhibited AtPI4Kbeta activity by approximately 90%. AtPI4Kbeta transcript levels were similar in all tissues analyzed. Light or treatment with hormones or salts did not change AtPI4Kbeta transcript levels to a great extent, indicating constitutive expression of the AtPI4Kbeta gene.  (+info)

Baculovirus expression and biochemical characterization of the human microsomal triglyceride transfer protein. (3/2544)

The microsomal triglyceride transfer protein (MTP) complexed to protein disulphide isomerase (PDI) is obligatory for the assembly of chylomicrons and very-low-density lipoproteins. The determination of the atomic structure of the MTP-PDI heterodimer has important implications for the treatment of those forms of hyperlipidaemia associated with the overproduction of very-low-density lipoproteins, which predispose to premature coronary heart disease. To perform structural studies of the human MTP-PDI complex it was necessary to produce milligram quantities of pure protein. We chose the baculovirus expression system for this purpose. Insects cells were co-infected with recombinant viruses encoding FLAG-tagged MTP and His-tagged PDI; the resulting heterodimer was purified by affinity chromatography. From 5 litres of insect cells, 4-6 mg of more than 95% pure recombinant protein was obtained. CD and attenuated total reflection Fourier-transform infrared spectroscopy indicate that the purified protein has around 34% alpha-helical and 33% beta-structure content. The recombinant protein had a comparable triglyceride transfer activity to that of bovine MTP-PDI. The production of polyclonal antibodies raised against the MTP and PDI subunits of the purified protein is described. The present study demonstrates the feasibility of expressing two proteins at high levels in insect cells and describes a transferable methodology for the purification of the resulting protein complex.  (+info)

A lipid modified ubiquitin is packaged into particles of several enveloped viruses. (4/2544)

An anti-ubiquitin cross-reactive protein which migrates more slowly (6.5 kDa) by SDS-PAGE than ubiquitin was identified in African swine fever virus particles. This protein was extracted into the detergent phase in Triton X-114 phase separations, showing that it is hydrophobic, and was radiolabelled with both [3H]palmitic acid and [32P]orthophosphate. This indicates that the protein has a similar structure to the membrane associated phosphatidyl ubiquitin described in baculovirus particles. A similar molecule was found in vaccinia virus and herpes simplex virus particles, suggesting that it may be a component of uninfected cell membranes, which is incorporated into membrane layers in virions during morphogenesis.  (+info)

Development of a Western blot assay for detection of bovine immunodeficiency-like virus using capsid and transmembrane envelope proteins expressed from recombinant baculovirus. (5/2544)

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera positive for bovine syncytial virus and bovine leukemia virus failed to recognize the recombinant fusion proteins, thereby showing the specificity of the BIV Western blot. One hundred and five bovine serum samples were tested for the presence of anti-BIV antibodies by the recombinant protein-based Western blot and a reference Western blot assay using cell culture-derived virions as test antigens. There was a 100% concordance when the p26 fusion protein was used in the Western blot. However, the Western blot using the tTM fusion protein as its test antigen identified four BIV-positive bovine sera which had tested negative in both the p26 recombinant-protein-based and the reference Western blot assays. This resulted in the lower concordance of 96.2% between the tTM-protein-based and reference Western blot assays. The results of this study showed that the recombinant p26 and tTM proteins can be used as test antigens for the serodetection of BIV-infection in animals.  (+info)

Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. (6/2544)

Although great progress has been made in the characterization of the biochemical and biological features of hepatitis C virus (HCV) gene expression, the elucidation of the HCV life cycle and the evaluation of novel antiviral strategies have been hindered by the lack of a suitable cell culture system. In this context, the development of an efficient HCV cDNA delivery method would contribute to the understanding of HCV replication. To assess the functionality of baculovirus mediated gene delivery for HCV expression, we have constructed recombinant baculoviruses encoding HCV cDNA under the control of the cytomegalovirus promoter. Transduction of the human hepatoma cell line Huh-7 with Bac-HCV vectors was efficient and HCV cDNA expression was enhanced by treatment of the infected cells with dexamethasone. HCV structural and nonstructural polypeptides were processed correctly and were found to localize in the cytoplasm in a pattern characteristic of the endoplasmic reticulum. The expression of the HCV proteins was detected for 49 days after infection. Thus, these results indicate that the recombinant Bac-HCV vectors are a useful tool for the delivery of HCV cDNA and can facilitate the analysis of structural and functional properties of the HCV proteins. In addition, the Bac-HCV vectors can provide important information on the evaluation of novel anti-HCV antiviral strategies.  (+info)

Reovirus virion-like particles obtained by recoating infectious subvirion particles with baculovirus-expressed sigma3 protein: an approach for analyzing sigma3 functions during virus entry. (7/2544)

Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a partial way for the major outer-capsid protein sigma3, we obtained in vitro assembly of large numbers of virion-like particles by binding baculovirus-expressed sigma3 protein to infectious subvirion particles (ISVPs) that lack sigma3. A level of sigma3 binding approaching 100% of that in native virions was routinely achieved. The sigma3 coat in these recoated ISVPs (rcISVPs) appeared very similar to that in virions by electron microscopy and three-dimensional image reconstruction. rcISVPs retained full infectivity in murine L cells, allowing their use to study sigma3 functions in virus entry. Upon infection, rcISVPs behaved identically to virions in showing an extended lag phase prior to exponential growth and in being inhibited from entering cells by either the weak base NH4Cl or the cysteine proteinase inhibitor E-64. rcISVPs also mimicked virions in being incapable of in vitro activation to mediate lysis of erythrocytes and transcription of the viral mRNAs. Last, rcISVPs behaved like virions in showing minor loss of infectivity at 52 degrees C. Since rcISVPs contain virion-like levels of sigma3 but contain outer-capsid protein mu1/mu1C mostly cleaved at the delta-phi junction as in ISVPs, the fact that rcISVPs behaved like virions (and not ISVPs) in all of the assays that we performed suggests that sigma3, and not the delta-phi cleavage of mu1/mu1C, determines the observed differences in behavior between virions and ISVPs. To demonstrate the applicability of rcISVPs for genetic studies of protein functions in reovirus entry (an approach that we call recoating genetics), we used chimeric sigma3 proteins to localize the primary determinants of a strain-dependent difference in sigma3 cleavage rate to a carboxy-terminal region of the ISVP-bound protein.  (+info)

Production and characterization of a soluble, active form of Tva, the subgroup A avian sarcoma and leukosis virus receptor. (8/2544)

The receptor for the subgroup A avian sarcoma and leukosis viruses [ASLV(A)] is the cellular glycoprotein Tva. A soluble form of Tva, sTva, was produced and purified with a baculovirus expression system. Using this system, 7 to 10 mg of purified sTva per liter of cultured Sf9 cells was obtained. Characterization of the carbohydrate modification of sTva revealed that the three N glycosylation sites in sTva were differentially utilized; however, the O glycosylation common to Tva produced in mammalian and avian cells was not observed. Purified sTva demonstrates significant biological activity, specifically blocking infection of avian cells by ASLV(A) with a 90% inhibitory concentration of approximately 25 pM. A quantitative enzyme-linked immunosorbent assay, developed to assess the binding of sTva to ASLV envelope glycoprotein, demonstrates that sTva has a high affinity for EnvA, with an apparent dissociation constant of approximately 0.3 nM. Once they are bound, a very stable complex is formed between EnvA and sTva, with an estimated complex half-life of 6 h. The soluble receptor protein described here represents a valuable tool for analysis of the receptor-envelope glycoprotein interaction and for structural analysis of Tva.  (+info)

ICTV Report: Baculoviridae Viralzone: Baculoviridae Index of Viruses - Baculoviridae (2006). In: ICTVdB - The Universal Virus ... Baculoviridae is a family of viruses. Arthropods, among the most studied being Lepidoptera, Hymenoptera and Diptera, serve as ... ISBN 978-0-306-45641-1. Wikimedia Commons has media related to Baculoviridae. Wikispecies has information related to ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ...
... is a genus of viruses, in the family Baculoviridae. Hymenoptera serve as natural hosts. There are two species ... "ICTV Report Baculoviridae". "Viral Zone". ExPASy. Retrieved 15 June 2015. "Virus Taxonomy: 2020 Release". International ... ICTV Report: Baculoviridae Viralzone: Gammabaculovirus (Articles with short description, Short description matches Wikidata, ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. " ...
... is a genus of viruses in the family Baculoviridae. The natural hosts of species in this family are ... ICTV Report: Baculoviridae Viralzone: Alphabaculovirus (Articles with short description, Short description matches Wikidata, ... "ICTV Report". ICTV Report Baculoviridae. "Viral Zone". ExPASy. Retrieved 12 June 2015. "Virus Taxonomy: 2020 Release". ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "ICTV Report". ...
"Baculoviridae ~ ViralZone page". viralzone.expasy.org. Retrieved 2020-05-10. Lupiani B, Raina AK, Huber C (January 1999). " ...
... is a genus of viruses, in the family Baculoviridae. Mosquito larvae serve as natural hosts. There is only one ... ICTV Report: Baculoviridae Viralzone: Deltabaculovirus (Articles with short description, Short description matches Wikidata, ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ... Use dmy dates from April 2017, Articles with 'species' microformats, Baculoviridae, Virus genera). ...
... is a genus of viruses, in the family Baculoviridae. Arthropods serve as natural hosts. There are 26 species in ... ICTV Report: Baculoviridae Viralzone: Betabaculovirus (Articles with short description, Short description matches Wikidata, Use ... Baculoviridae". The Journal of General Virology. 99 (9): 1185-1186. doi:10.1099/jgv.0.001107. PMID 29947603. "Viral Zone". ... dmy dates from April 2017, Articles with 'species' microformats, Baculoviridae, Virus genera). ...
Members of Baculoviridae also encode a PCNA homolog (P11038). The viral gp45 sliding clamp subunit protein contains two domains ...
Baculoviridae and Rickettsia species infect nursery web spiders, as well. They most likely enter the gastrointestinal tract via ...
... (CpGV) is a granulovirus belonging to the family Baculoviridae. It has a double-stranded DNA ...
They are members of the Baculoviridae, a family that is restricted to insects. Most baculovirus isolates have been obtained ...
... contains the following three families: Baculoviridae Hytrosaviridae Nudiviridae "Virus Taxonomy: 2020 Release". ...
From the family Baculoviridae, it is a type of Alphabaculovirus and its genome is 80-180kb long. NPVs are commonly used as ...
... hytrosaviruses are similar to members of other arthropod-infecting virus families such as Baculoviridae, Nudiviridae and ...
Baculoviridae Viruses from the family Baculoviridae induce in their hosts changes to both feeding behavior and environment ...
Baculoviridae - members of this family may also cause polyhedrosis Betairidovirinae - members of this subfamily may also cause ... a genus of arthropod viruses in the family Baculoviridae. However, cypoviruses have an RNA genome and replicate in the ...
The NPVs come under the family of baculoviridae and its virions are enveloped rod shaped nucleocapsids containing circular, ...
Since baculoviridae infect only insects and not humans, the function of P35 in the immune evasion of infected cells is not ...
Both in America and in Europe, research continues into biological control of the species, and for example the Baculoviridae ...
... (AcMNPV) is a nucleopolyhedrovirus belonging to the family Baculoviridae. ...
Baculoviridae), respectively. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses (2011) ISBN ...
Baculoviridae, Insect viral diseases, Lymantria dispar). ...
... but the term now refers to 35 species of the family Baculoviridae-mostly alphabaculoviruses, but also one deltabaculovirus and ...
... where she studied the expression of insect cells using baculoviridae. In 1992 Jansen moved back to the United States, where she ...
Ascoviridae Asfarviridae Aspiviridae Astroviridae Atkinsviridae Autographiviridae Avsunviroidae Bacilladnaviridae Baculoviridae ...
Baculoviridae, Bicaudaviridae, Clavaviridae, Fuselloviridae, Globuloviridae, Guttaviridae, Halspiviridae, Hytrosaviridae, ...
Expression System: Baculoviridae. *Mutation(s): No *Deposited: 2009-12-04 Released: 2010-06-23 ...
Baculovirus use Baculoviridae Baculoviruses use Baculoviridae Bad Protein use bcl-Associated Death Protein ...
Gaucher disease is an inherited sphingolipidosis resulting from deficient glucocerebrosidase activity. Three clinical forms of Gaucher disease have been described: type 1 as non-neuronopathic, type 2 as acute neuronopathic, and type 3 as subacute neuronopathic. We recently identified a rare mutation …
MeSH Terms: Allosteric Regulation; Amino Acid Sequence; Animals; Ascorbic Acid/metabolism; Baculoviridae/genetics; Binding ...
Baculoviridae Preferred Term Term UI T048904. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1991). ... Baculoviridae Preferred Concept UI. M0025032. Registry Number. txid10442. Scope Note. Family of INSECT VIRUSES which contain ... Baculoviridae. Tree Number(s). B04.280.065. B04.525.100. Unique ID. D016367. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/ ...
Dive into the research topics of Gain of structure and IgE epitopes by eukaryotic expression of the major Timothy grass pollen allergen, Phl p 1. Together they form a unique fingerprint. ...
Baculoviridae - Preferred Concept UI. M0025032. Scope note. Family of INSECT VIRUSES which contain polyhedron-shaped or ...
Baculoviridae Preferred Term Term UI T048904. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1991). ... Baculoviridae Preferred Concept UI. M0025032. Registry Number. txid10442. Scope Note. Family of INSECT VIRUSES which contain ... Baculoviridae. Tree Number(s). B04.280.065. B04.525.100. Unique ID. D016367. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/ ...
Baculoviridae (20) *Mostrar más.... Tipo de estudio * Prognostic_studies (189) * Risk_factors_studies (61) ...
Findings of significant congruence between phylogenies of hantaviruses and phylogenies of their rodent reservoirs have led to the theory that rodents, although infected by the virus, are not harmed by it because of long-standing hantavirus-rodent host coevolution,[18][19] although findings in 2008 led to new hypotheses regarding hantavirus evolution.[20][21] Various hantaviruses have been found to infect multiple rodent species, and cases of cross-species transmission (host switching) have been recorded.[22][23][24] Additionally, rates of substitution based on nucleotide sequence data reveal that hantavirus clades and rodent subfamilies may not have diverged at the same time.[21][25] Furthermore, as of 2007 hantaviruses have been found in multiple species of non-rodent shrews and moles.[21][26][27][28] Taking into account the inconsistencies in the theory of coevolution, it was proposed in 2009 that the patterns seen in hantaviruses in relation to their reservoirs could be attributed to ...
AN - coord with specific insect /virol HN - 2002; use BACULOVIRIDAE 1997-2001; for GRANULOSIS VIRUSES use BACULOVIRIDAE 1991- ... A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by ovicylindrical occlusion bodies. The type ...
Heaton, N. S., Moshkina, N., Fenouil, R., Gardner, T. J., Aguirre, S., Shah, P. S., Zhao, N., Manganaro, L., Hultquist, J. F., Noel, J., Sachs, D., Hamilton, J., Leon, P. E., Chawdury, A., Tripathi, S., Melegari, C., Campisi, L., Hai, R., Metreveli, G., Gamarnik, A. V., & 11 othersGarcía-Sastre, A., Greenbaum, B., Simon, V., Fernandez-Sesma, A., Krogan, N. J., Mulder, L. C. F., van Bakel, H., Tortorella, D., Taunton, J., Palese, P. & Marazzi, I., 16 Feb 2016, In: Immunity. 44, 2, p. 438 1 p.. Research output: Contribution to journal › Comment/debate ...
Välkommen till lookformedical.com, där du kan ställa frågor och få svar från andra användare ...
Wang, S. F., Tseng, S. P., Loh, E. W., Wang, W. H., Li, M. C., Chen, K. H., Tsai, W. C., Lee, Y. M., Chen, H. Y., Liu, F. T., Arthur Chen, Y. M. & Huang, J. C., 1 2月 2017, 於: Developmental and Comparative Immunology. 67, p. 8-17 10 p.. 研究成果: Article › 同行評審 ...
Many parasites manipulate host behaviour to enhance parasite transmission and survival. A fascinating example is baculoviruses, which often induce death in caterpillar hosts at elevated positions (tree-top disease). To date, little is known about the underlying processes leading to this adaptive host manipulation. Here, we show that the baculovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) triggers a positive phototactic response in S. exigua larvae prior to death and causes the caterpillars to die at elevated positions. This light-dependent climbing behaviour is specific for infected larvae, as movement of uninfected caterpillars during larval development was light-independent. We hypothesize that upon infection, SeMNPV captures a host pathway involved in phototaxis and/or light perception to induce this remarkable behavioural change ...
Baculoviridae. Nonocluded Baculo viruses. In Atlas of invertebrate Viruses (Adam, J. R. and Bonawi, eds.) CRS Press: pp 187-319 ...
Baculoviridae Medicine & Life Sciences 66% * hemagglutinins Agriculture & Biology 62% * Insecta Medicine & Life Sciences 58% ...
Top research keywords: Anoplophora Glabripennis, Coleoptera, Insecta, Larva, Baculoviridae. Kathleen Brown. Professor, Plant ...
In this concept cloud, the sizes of the concepts are based not only on the number of corresponding publications, but also how relevant the concepts are to the overall topics of the publications, how long ago the publications were written, whether the person was the first or senior author, and how many other people have written about the same topic. The largest concepts are those that are most unique to this person ...
HN - 2020; for ORYCTES VIRUSES use BACULOVIRIDAE 1991-2019 BX - Nudivirus BX - Nudiviruses BX - Oryctes Viruses MH - Obesity, ... and circular dsDNA viruses that share structural and replication characteristics with BACULOVIRIDAE. Genera include ...
This graph shows the total number of publications written about "Guttaviridae" by people in this website by year, and whether "Guttaviridae" was a major or minor topic of these publications ...
... within the Baculoviridae, or a representative of a new virus family, Whispoviridae [129]. Since 2002 the ICTV included WSSV as ... family Baculoviridae, where it would be formally named PmNOBII, as the second non-occluded baculovirus (NOB) reported for a ...
Baculoviridae) on the Egyptian Cotton Leaf worm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). Ecol Balk 6(2):93- ...
Arboviruses - Arthropod-borne Viruses Astroviruses - Gastroenteritis Baculoviridae Bunyaviridae - Hantavirus Caliciviridae ...
Baculoviridae - Barnaviridae - Benyviridae - Bicaudaviridae - Bidnaviridae - Birnaviridae - Bromoviridae - Caliciviridae - ...
Worldwide source of medical classification resources ...
Viral Genome Organizer (VGO) is an easy-to-use Java genome browser. It can be used to display information about a genome, including its genes, ORFs and start/stop codons. It can also be used to perform a regular expression search, a fuzzy motif search, and a masslist search. Because VGO talks to our database of genome sequences (VOCs) it can be used to compare genomes.. ...
This graph shows the total number of publications written about "Tectiviridae" by people in this website by year, and whether "Tectiviridae" was a major or minor topic of these publications ...
Adenoviridae, Arenaviridae, Astroviridae, Baculoviridae, Birnaviridae, Bornaviridae, Bunyaviridae, Caliciviridae, Circoviridae ...
  • Los vectores baculovirus se emplean para la expresión de genes extraños en insectos y como biopesticidas. (bvsalud.org)

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