Gram-negative bacteria occurring in the lower intestinal tracts of man and other animals. It is the most common species of anaerobic bacteria isolated from human soft tissue infections.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
Infections with bacteria of the genus BACTEROIDES.
A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium has been isolated from the mouth, urine, feces, and infections of the mouth, soft tissue, respiratory tract, urogenital tract, and intestinal tract. It is pathogenic, but usually in association with other kinds of organisms.
A genus of gram-negative, anaerobic, rod-shaped bacteria found in cavities of humans and other animals. No endospores are formed. Some species are pathogenic and occur in various purulent or gangrenous infections.
An antibacterial agent that is a semisynthetic analog of LINCOMYCIN.
A semisynthetic cephamycin antibiotic resistant to beta-lactamase.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Accumulation of purulent material in tissues, organs, or circumscribed spaces, usually associated with signs of infection.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS. It has also been proposed as a radiation sensitizer for hypoxic cells. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985, p133), this substance may reasonably be anticipated to be a carcinogen (Merck, 11th ed).
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Substances that reduce the growth or reproduction of BACTERIA.
Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.
A semisynthetic cephamycin antibiotic that is administered intravenously or intramuscularly. The drug is highly resistant to a broad spectrum of beta-lactamases and is active against a wide range of both aerobic and anaerobic gram-positive and gram-negative microorganisms.
A genus of gram-negative, anaerobic, nonsporeforming, nonmotile rods. Organisms of this genus had originally been classified as members of the BACTEROIDES genus but overwhelming biochemical and chemical findings in 1990 indicated the need to separate them from other Bacteroides species, and hence, this new genus was established.
A genus of gram-positive, anaerobic, coccoid bacteria that is part of the normal flora of humans. Its organisms are opportunistic pathogens causing bacteremias and soft tissue infections.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
An abscess located in the abdominal cavity, i.e., the cavity between the diaphragm above and the pelvis below. (From Dorland, 27th ed)
Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A large group of anaerobic bacteria which show up as pink (negative) when treated by the Gram-staining method.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A pathologic process consisting in the formation of pus.
Naturally occurring family of beta-lactam cephalosporin-type antibiotics having a 7-methoxy group and possessing marked resistance to the action of beta-lactamases from gram-positive and gram-negative organisms.
Broad- spectrum beta-lactam antibiotic similar in structure to the CEPHALOSPORINS except for the substitution of an oxaazabicyclo moiety for the thiaazabicyclo moiety of certain CEPHALOSPORINS. It has been proposed especially for the meningitides because it passes the blood-brain barrier and for anaerobic infections.
Beta-lactam antibiotics that differ from PENICILLINS in having the thiazolidine sulfur atom replaced by carbon, the sulfur then becoming the first atom in the side chain. They are unstable chemically, but have a very broad antibacterial spectrum. Thienamycin and its more stable derivatives are proposed for use in combinations with enzyme inhibitors.
A genus of gram-negative, anaerobic, nonsporeforming, nonmotile rods or coccobacilli. Organisms in this genus had originally been classified as members of the BACTEROIDES genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Bacteroides species, and hence, this new genus was created.
A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
Polysaccharides found in bacteria and in capsules thereof.
A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.
A building block of penicillin, devoid of significant antibacterial activity. (From Merck Index, 11th ed)
Infections by bacteria, general or unspecified.
A semisynthetic cephalosporin antibiotic which can be administered intravenously or by suppository. The drug is highly resistant to a broad spectrum of beta-lactamases and is active against a wide range of both aerobic and anaerobic gram-positive and gram-negative organisms. It has few side effects and is reported to be safe and effective in aged patients and in patients with hematologic disorders.
A family of gram-negative bacteria found primarily in the intestinal tracts and mucous membranes of warm-blooded animals. Its organisms are sometimes pathogenic.
The functional hereditary units of BACTERIA.
A naphthacene antibiotic that inhibits AMINO ACYL TRNA binding during protein synthesis.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
Cyclic AMIDES formed from aminocarboxylic acids by the elimination of water. Lactims are the enol forms of lactams.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins found in any species of bacterium.
Bacteria which retain the crystal violet stain when treated by Gram's method.
Four-membered cyclic AMIDES, best known for the PENICILLINS based on a bicyclo-thiazolidine, as well as the CEPHALOSPORINS based on a bicyclo-thiazine, and including monocyclic MONOBACTAMS. The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A semisynthetic cephamycin antibiotic with a broad spectrum of activity against both gram-positive and gram-negative microorganisms. It has a high rate of efficacy in many types of infection and to date no severe side effects have been noted.
A plant genus in the family PINACEAE, order Pinales, class Pinopsida, division Coniferophyta. It is the source of cedarwood oil. Cedar ordinarily refers to this but also forms part of the name of plants in other genera.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.
Semisynthetic, broad-spectrum, AMPICILLIN derived ureidopenicillin antibiotic proposed for PSEUDOMONAS infections. It is also used in combination with other antibiotics.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A beta-lactamase inhibitor with very weak antibacterial action. The compound prevents antibiotic destruction of beta-lactam antibiotics by inhibiting beta-lactamases, thus extending their spectrum activity. Combinations of sulbactam with beta-lactam antibiotics have been used successfully for the therapy of infections caused by organisms resistant to the antibiotic alone.
Acids, salts, and derivatives of clavulanic acid (C8H9O5N). They consist of those beta-lactam compounds that differ from penicillin in having the sulfur of the thiazolidine ring replaced by an oxygen. They have limited antibacterial action, but block bacterial beta-lactamase irreversibly, so that similar antibiotics are not broken down by the bacterial enzymes and therefore can exert their antibacterial effects.
A genus of gram-positive, anaerobic, coccoid bacteria that is part of the normal flora of the mouth, upper respiratory tract, and large intestine in humans. Its organisms cause infections of soft tissues and bacteremias.
Infections with bacteria of the genus FUSOBACTERIUM.
Clavulanic acid and its salts and esters. The acid is a suicide inhibitor of bacterial beta-lactamase enzymes from Streptomyces clavuligerus. Administered alone, it has only weak antibacterial activity against most organisms, but given in combination with other beta-lactam antibiotics it prevents antibiotic inactivation by microbial lactamase.
Semisynthetic broad-spectrum cephalosporin.
An antibiotic derived from penicillin similar to CARBENICILLIN in action.
An antibiotic produced by Streptomyces lincolnensis var. lincolnensis. It has been used in the treatment of staphylococcal, streptococcal, and Bacteroides fragilis infections.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.

The in-vitro activity of linezolid (U-100766) and tentative breakpoints. (1/843)

The in-vitro activity of linezolid, a novel oxazolidinone, was investigated in comparison with those of amoxycillin, cefuroxime, quinupristin/dalfopristin, trovafloxacin and vancomycin against 420 recent Gram-positive and anaerobic clinical isolates. Linezolid was equally active (MIC90 1 mg/L) against methicillin-susceptible and -resistant Staphylococcus aureus. It demonstrated uniform activity against streptococci and enterococci and no cross-resistance with other agents. The time-kill kinetic data demonstrated that the in-vitro activity of linezolid was predominantly bacteriostatic; slow bactericidal activity was only observed at the higher concentration with streptococci. An increase in inoculum from 10(4) to 10(6) cfu on selected strains had little effect on the MICs (MIC90 within one dilution step) of linezolid and an increase in inoculum from 10(5) to 10(7) cfu/mL had no notable effect on the in-vitro bactericidal activity. A tentative linezolid breakpoint of 2 mg/L was chosen after analysis of distribution of susceptibilities.  (+info)

Bacteroides fragilis toxin 2 damages human colonic mucosa in vitro. (2/843)

BACKGROUND: Strains of Bacteroides fragilis producing a 20 kDa protein toxin (B fragilis toxin (BFT) or fragilysin) are associated with diarrhoea in animals and humans. Although in vitro results indicate that BFT damages intestinal epithelial cells in culture, the effects of BFT on native human colon are not known. AIMS: To examine the electrophysiological and morphological effects of purified BFT-2 on human colonic mucosa in vitro. METHODS: For resistance (R) measurements, colonic mucosa mounted in Ussing chambers was exposed to luminal or serosal BFT-2 (1.25-10 nM) and after four hours morphological damage was measured on haematoxylin and eosin stained sections using morphometry. F actin distribution was assessed using confocal microscopy. RESULTS: Serosal BFT-2 for four hours was four-, two-, seven-, and threefold more potent than luminal BFT-2 in decreasing resistance, increasing epithelial 3H-mannitol permeability, and damaging crypt and surface colonocytes, respectively (p<0.05). Confocal microscopy showed reduced colonocyte F actin staining intensity after exposure to BFT-2. CONCLUSIONS: BFT-2 increases human colonic permeability and damages human colonic epithelial cells in vitro. These effects may be important in the development of diarrhoea and intestinal inflammation caused by B fragilis in vivo.  (+info)

Microbiological and inflammatory effects of murine recombinant interleukin-10 in two models of polymicrobial peritonitis in rats. (3/843)

A protective effect of interleukin-10 (IL-10) against the development of lethal shock has been demonstrated in various animal models. In contrast, the immunosuppressant properties of this mediator have been minimally evaluated in low-mortality models of infections. The clinical, microbiological, and inflammatory effects of murine recombinant IL-10 (mrIL-10) therapy were evaluated in two models of peritonitis in rats, which differed in the degree of severity of peritoneal inflammation 3 days after inoculation of Escherichia coli and Bacteroides fragilis with or without Enterococcus faecalis. The severity of the disease remained unchanged compared to that in control animals. A dose-related decrease in the peritoneal phagocyte count was observed in the treated groups compared to the counts in control animals. The subsequent experiments were performed exclusively in the mixed gram-positive-gram negative model, which exhibits an intense and prolonged inflammatory response with similar criteria. The early effects of mrIL-10 (evaluated 6 h after inoculation), repeated injections of mrIL-10 (four doses injected from 0 to 9 h after bacterial challenge), and pretreatment (two doses injected 6 and 3 h before inoculation) were evaluated. The clinical and microbiological parameters remained unchanged in the treated animals. Decreases in the peritoneal phagocyte count and the peritoneal concentration of tumor necrosis factor were observed following repeated injections of mrIL-10. In summary, our data suggest that mrIL-10 does not worsen the manifestations of sepsis. However, these results need to be confirmed in clinical practice.  (+info)

Antimicrobial resistance patterns of Bacteroides fragilis group organisms in Korea. (4/843)

Antimicrobial resistance patterns of 913 clinical isolates of Bacteroides fragilis group organisms were monitored during an 8-year period in Korea. In general the resistance rates of the non-fragilis B. fragilis group species were higher than those of B. fragilis for all the drugs tested. The rate of resistance to clindamycin remarkably increased and those to some beta-lactam drugs such as piperacillin and cefotaxime also increased. No isolates were found to be resistant to imipenem, metronidazole, or chloramphenicol. beta-lactam and beta-lactamase inhibitor combinations and cefoxitin were more active than the other beta-lactams. Therefore, these agents may be considered when empirical selection of antimicrobial agents is required to treat severe anaerobic infections.  (+info)

Diversity of bacteroides fragilis strains in their capacity to recover phages from human and animal wastes and from fecally polluted wastewater. (5/843)

Great differences in capability to detect bacteriophages from urban sewage of the area of Barcelona existed among 115 strains of Bacteroides fragilis. The capability of six of the strains to detect phages in a variety of feces and wastewater was studied. Strains HSP40 and RYC4023 detected similar numbers of phages in urban sewage and did not detect phages in animal feces. The other four strains detected phages in the feces of different animal species and in wastewater of both human and animal origin. Strain RYC2056 recovered consistently higher counts than the other strains and also detected counts ranging from 10(1) to approximately 10(3) phages per ml in urban sewage from different geographical areas. This strain detected bacteriophages in animal feces even though their relative concentration with respect to the other fecal indicators was significantly lower in wastewater polluted with animal feces than in urban sewage.  (+info)

Bacteroides fragilis transfer factor Tn5520: the smallest bacterial mobilizable transposon containing single integrase and mobilization genes that function in Escherichia coli. (6/843)

Many bacterial genera, including Bacteroides spp., harbor mobilizable transposons, a class of transfer factors that carry genes for conjugal DNA transfer and, in some cases, antibiotic resistance. Mobilizable transposons are capable of inserting into and mobilizing other, nontransferable plasmids and are implicated in the dissemination of antibiotic resistance. This paper presents the isolation and characterization of Tn5520, a new mobilizable transposon from Bacteroides fragilis LV23. At 4,692 bp, it is the smallest mobilizable transposon reported from any bacterial genus. Tn5520 was captured from B. fragilis LV23 by using the transfer-deficient shuttle vector pGAT400DeltaBglII. The termini of Tn5520 contain a 22-bp imperfect inverted repeat, and transposition does not result in a target site repeat. Tn5520 also demonstrates insertion site sequence preferences characterized by A-T-rich nucleotide sequences. Tn5520 has been sequenced in its entirety, and two large open reading frames whose predicted protein products exhibit strong sequence similarity to recombinase-integrase enzymes and mobilization proteins, respectively, have been identified. The transfer, mobilization, and transposition properties of Tn5520 have been studied, revealing that Tn5520 mobilizes plasmids in both B. fragilis and Escherichia coli at high frequency and also transposes in E. coli.  (+info)

TNF-binding protein ameliorates inhibition of skeletal muscle protein synthesis during sepsis. (7/843)

We examined the effects of TNF-binding protein (TNFBP) on regulatory mechanisms of muscle protein synthesis during sepsis in four groups of rats: Control; Control+TNFBP; Septic; and Septic+TNFBP. Saline (1. 0 ml) or TNFBP (1 mg/kg, 1.0 ml) was injected daily starting 4 h before the induction of sepsis. The effect of TNFBP on gastrocnemius weight, protein content, and the rate of protein synthesis was examined 5 days later. Sepsis reduced the rate of protein synthesis by 35% relative to controls by depressing translational efficiency. Decreases in protein synthesis were accompanied by similar reductions in protein content and muscle weight. Treatment of septic animals with TNFBP for 5 days prevented the sepsis-induced inhibition of protein synthesis and restored translational efficiency to control values. TNFBP treatment of Control rats for 5 days was without effect on muscle protein content or protein synthesis. We also assessed potential mechanisms regulating translational efficiency. The phosphorylation state of p70(S6) kinase was not altered by sepsis. Sepsis reduced the gastrocnemius content of eukaryotic initiation factor 2Bepsilon (eIF2Bepsilon), but not eIF2alpha. The decrease in eIF2Bepsilon content was prevented by treatment of septic rats with TNFBP. TNFBP ameliorates the sepsis-induced changes in protein metabolism in gastrocnemius, indicating a role for TNF in the septic process. The data suggest that TNF may impair muscle protein synthesis by reducing expression of specific initiation factors during sepsis.  (+info)

Structural consequences of the active site substitution Cys181 ==> Ser in metallo-beta-lactamase from Bacteroides fragilis. (8/843)

The metallo-beta-lactamases require divalent cations such as zinc or cadmium for hydrolyzing the amide bond of beta-lactam antibiotics. The crystal structure of the Zn2+ -bound enzyme from Bacteroides fragilis contains a binuclear zinc center in the active site. A hydroxide, coordinated to both zinc atoms, is proposed as the moiety that mounts the nucleophilic attack on the carbonyl carbon atom of the beta-lactam bond of the substrate. It was previously reported that the replacement of the active site Cys181 by a serine residue severely impaired catalysis while atomic absorption measurements indicated that binding of the two zinc ions remained intact. Contradicting data emerge from recent mass spectrometry results, which show that only a single zinc ion binds to the C181S metallo-beta-lactamase. In the current study, the C181S mutant enzyme was examined at the atomic level by determining the crystal structure at 2.6 A resolution. The overall structure of the mutant enzyme is the same as that of the wild-type enzyme. At the mutation site, the side chain of Ser181 occupies the same position as that of the side chain of Cys181 in the wild-type protein. One zinc ion, Zn1, is present in the crystal structure; however, the site of the second zinc ion, Zn2 is unoccupied. A water molecule is associated with Zn1, reminiscent of the hydroxide seen in the structure of the wild-type enzyme but farther from the metal. The position of the water molecule is off the plane of the carboxylate group of Asp103; therefore, the water molecule may be less nucleophilic than a water molecule which is coplanar with the carboxylate group.  (+info)

TY - JOUR. T1 - Molecular evolution of the pathogenicity island of enterotoxigenic Bacteroides fragilis strains. AU - Franco, Augusto A.. AU - Cheng, Rodney K.. AU - Chung, Gyung Tae. AU - Wu, Shaoguang. AU - Oh, Hee Bok. AU - Sears, Cynthia Louise. PY - 1999/11. Y1 - 1999/11. N2 - Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have been associated with diarrheal disease in animals and young children. Studying a collection of ETBF and nontoxigenic B. fragilis (NTBF) strains, we found that bft and a second metalloprotease gene (mpII) are contained in an ~6-kb pathogenicity island (termed B. fragilis pathogenicity island or BfPAI) which is present exclusively in all 113 ETBF strains tested (pattern I). Of 191 NTBF strains, 100 (52%) lack both the BfPAI and at least a 12-kb region flanking BfPAI (pattern II), and 82 of 191 NTBF strains (43%) lack the BfPAI but contain the flanking region (pattern III). The nucleotide sequence flanking ...
Bacteroides species, saccharolytic Gram-negative obligate anaerobes, are frequently isolated from human infections such as peritonitis, abscesses and bacteremia. Among the species in the genus Bacteroides, thespecies called B. fragilis group areparticularly involved inhuman infections andaremedically important because they account for a major part of anaerobic isolates from clinical specimens. The purpose of this study was to develop PCR primers that specifically and simultaneously amplify theβ-isopropylmalate dehydrogenase gene leuB in B. fragilis group species. We determined partial nucleotide sequences of leuB genes and compared them in seventeen strains of nine B. fragilis group species, and the regions that are conserved among Bacteroides strains but different from other species were used as a B. fragilis group-specific PCR primer set, BacLBF-BacLBR. Specificity tests of the primer set using 52 phenotypically characterized strains and 75 isolates from rat feces showed only one case each ...
We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other gastrointestinal disorders. The organism was detected in 11 (13.2%) of 83 patients with inflammatory bowel disease. Of 57 patients with active disease, 19.3% were toxin positive; none of those with inactive disease had specimens positive for enterotoxigenic Bacteroides fragilis gene sequences.
The results of a multicenter US survey using the National Committee for Clinical Laboratory Standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of Bacteroides fragilis group species were compared from 1997 to 2000. The test panel consisted of 14 antibiotics: 3 carbapenems, 3 β-lactam-β-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. Declines in the geometric mean minimum inhibitory concentrations were seen with imipenem, meropenem, ampicillin-sulbactam, and the cephamycins. Increased geometric means were observed with the fluoroquinolones and were usually accompanied by an increase in resistance rates. Bacteroides distasonis shows the highest resistance rates among β-lactam antibiotics, whereas Bacteroides vulgatus shows the highest resistance levels among fluoroquinolones. B. fragilis shows the lowest resistance rates for all antibiotics. All strains were susceptible to chloramphenicol ...
Of 1284 Bacteroides strains collected in Europe in 2000 for antibiotic susceptibility surveillance, 65 isolates displayed imipenem minimum inhibitory concentrations (MICs) , or =1 mg/L and were chosen for a thorough analysis of their resistance mechanism. Twenty-five of the isolates were positive for the cfiA carbapenem resistance gene. The resistance rates were 0.8% and 1.3% for imipenem and meropenem, respectively. In six of the strains, insertion sequence (IS) elements (IS613, IS614B, IS1186 and IS1187) activated the cfiA gene. However, other strains displayed at least elevated carbapenem MICs or were carbapenem resistant and produced measurable carbapenemase activities but did not harbour IS elements in the region upstream of the cfiA gene. The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-beta-lactamase via activation of the cfiA gene by IS elements (higher level resistance) or by activation of its putative own promoter.. ...
Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads.|br| We sequenced |em|Bacteroides fragilis|/em| strain BE1 using both the Illumina MiSeq and Oxford Nanopore MinION platforms. We were able to assemble a single chromosome of 5.18 Mb, with no gaps, using publicly available software and commodity computing hardware. We identified gene rearrangements and the state of invertible promoters in the strain. |br| The final assembly and annotations are available from the public archives (EBI/NCBI/DDBJ) and additionally here we provide the assemblies done for comparison from each of the individual technologies and the alignments of the original raw reads to the final assembly.|br|
The polysaccharide capsule of Bacteroides fragilis has been shown to be important in the virulence of the organism. The capsular polysaccharide (CP) of B. fragilis has been extensively purified. Using a murine model of intraabdominal abscess formation, we have been able to demonstrate cellular immunity to the capsular polysaccharide of B. fragilis. Immunization of C57BL/10J mice with the CP over 5 wk prevents abscess formation when the mice are challenged with B. fragilis intraperitoneally. This immunity can be transferred to naive mice with spleen cells from immune animals. The immune cells bear Thy-1.2 and Ly-2.2 antigens. The immune response has been shown to be antigen specific, but not H-2 restricted. The possibility that these immune cells are suppressor T cells is discussed. The experimental system presented provides a model for the examination of the cellular interactions responsible for abscess formation and the cellular response to bacterial pathogens. ...
We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other gastrointestinal disorders. The organism was detected in 11 (13.2%) of 83 patients with inflammatory bowel disease. Of 57 patients with active disease, 19.3% were toxin positiv …
Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most ...
Accepted name: 4-O-β-D-mannosyl-D-glucose phosphorylase. Reaction: 4-O-β-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + α-D-mannose 1-phosphate. Glossary: 4-O-β-D-mannopyranosyl-D-glucopyranose = β-D-mannopyranosyl-(1→4)-D-glucopyranose. Other name(s): mannosylglucose phosphorylase. Systematic name: 4-O-β-D-mannopyranosyl-D-glucopyranose:phosphate α-D-mannosyltransferase. Comments: This enzyme forms part of a mannan catabolic pathway in the anaerobic bacterium Bacteroides fragilis NCTC 9343.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: References:. 1. Senoura, T., Ito, S., Taguchi, H., Higa, M., Hamada, S., Matsui, H., Ozawa, T., Jin, S., Watanabe, J., Wasaki, J. and Ito, S. New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase. Biochem. Biophys. Res. Commun. 408 (2011) 701-706. [PMID: 21539815]. ...
Bacteroides fragilis is a Gram-negative member of the normal human gut microbiota. The Bacteroidetes constitutes one of the major bacterial phyla in the healthy human gut [1]. However, B. fragilis is also an important opportunistic pathogen, and it is the most frequently isolated anaerobic bacterium in clinical specimens, including abdominal abscesses and bloodstream infections [2]. Indeed, while B. fragilis accounts for only 4 to 13% of the normal human fecal microbiota, it is responsible for 63 to 80% of Bacteroides infections [3]. Only a few virulence factors have been described for B. fragilis, with the best characterized being the polysaccharide (PS) capsule [4] and a secreted metalloprotease, fragilysin [5]. The capsule, which displays antigenic variation, promotes the formation of abscesses [4], and the reduction of pro-inflammatory responses to B. fragilis[4, 6]. The metalloprotease fragilysin, which has been linked to diarrheal disease [5], has activity against the zonula junctions ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
ETBF rapidly induces immune cell pStat3, which is independent of epithelial pStat3. This occurs before ETBF-induced mucosal permeability, suggesting that ETBF, likely through B. fragilis toxin and its action on the colonic epithelial cell, triggers mucosal immune cell Stat3 activation. Peak mucosal …
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The C10 family of cysteine proteases includes enzymes that contribute to the virulence of bacterial pathogens, such as SpeB in Streptococcus pyogenes. The presence of homologues of cysteine protease genes in human commensal organisms has not been examined. Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. These four protease genes, two were directly contiguous to open reading frames predicted to encode staphostatin-like inhibitors, with which the protease genes were co-transcribed. Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. Gene annotation indicated that one of these insertions was a conjugative Tn-like element and the other was a prophage-like element, which was shown to be capable of excision. Homologues of the B. fragilis C10
Bacteroides fragilis is an obligately anaerobic bacterium that can be isolated from a variety of human infections (2). In the mid-1980s it was recognized that some strains produce an enterotoxin (ET) that can cause acute diarrhea in humans, young lambs, calves, pigs, and foals (6,7). Enterotoxigenic B. fragilis (ETBF) strains have also been isolated from the feces of children with diarrhea (9). Kato et al. (4) showed that B. fragilis blood culture isolates were more likely to be ETBF and suggested that ETBF strains are more virulent than enterotoxin-negative strains. Recently, the enterotoxin gene of B. fragilis has been cloned, sequenced, and identified as producing a zinc metalloprotease of 44.4 kDa (5).. In order to determine the relative frequency of ETBF in different geographic locations, 93 B. fragilis clinical isolates from Germany and Southern California were analyzed. Two PCR assays were used to detect two independent genetic sequences of the B. fragilis enterotoxin gene. In addition, ...
We have cloned the Bacteroides fragilis TAL2480 neuraminidase (NANase) structural gene, nanH, in Escherichia coli. This was accomplished by using the cloning shuttle vector pJST61 and a partial Sau3A library of TAL2480 chromosomal inserts created in E. coli. The library was mobilized into the NANase-deficient B. fragilis TM4000 derivative TC2. NANase-producing colonies were enriched by taking advantage of the inability of TC2, but not the wild-type of NANase+ revertant, to grow in vitro in fluid aspirated from the rat granuloma pouch. Plasmids pJST61-TCN1 and pJST61-TCN3, containing inserts of 9.1 and 4.5 kilobases (kb), respectively, were found in the TC2 derivatives that grew in the rat pouch medium. In B. fragilis, NANase production from the two plasmids was inducible by free N-acetylneuraminic acid or sialic acid-containing substrates, just as in the parental TAL2480 strain. However, when these plasmids were transferred back to E. coli, NANase activity was barely detectable. A 3.5-kb portion ...
Bacteroides fragilis ATCC ® 25285™ Designation: VPI 2553 TypeStrain=True Application: Media testing Quality control strain Susceptibility testing Quality control strain for BBL and MicroScan ® products Pharmaceutical and Personal Care
Bacteroides fragilis ATCC ® 25285™ Designation: VPI 2553 TypeStrain=True Application: Media testing Quality control strain Susceptibility testing Quality control strain for BBL and MicroScan ® products Pharmaceutical and Personal Care
[Biological activity of Bacteroides fragilis].: The investigation was performed with 9 strains of B. fragilis isolated from pregnant women in their 38th week of
Electron microscopic studies of laboratory-passaged and animal-passaged isolates of Bacteroides fragilis showed significantly more capsular material around the latter. This observation correlated with increased survival of animal-passaged bacteria in rabbit intraperitoneal chambers and increased resistance to phagocytosis and opsonophagocytic killing by neutrophils. With an initial inoculum of 2.5 x 10(6) colony-forming units/ml, the number of bacteria surviving after incubation for 2 hr with neutrophils and pooled normal human serum was significantly (P less than 0.01) greater for animal-passaged than for laboratory-passaged bacteria. Neutrophil uptake of 14C-labeled animal-passaged and laboratory-passaged bacteria after incubation for 20 min was 45% and 63%, respectively (P less than 0.05). No significant difference in survival was found between animal-passaged and laboratory-passaged isolates of Bacteroides thetaiotaomicron. These findings suggest that on mechanism whereby the capsule of B. ...
Significantly more reads were assigned to the Bacteroidetes phylum in the experimental sample (36%) as compared to the control sample (8.2%). At the class level, Bacteroidia (1.3% in the control sample vs. 24.4% in the experimental sample) and Sphingobacteria (1.1% in the control sample vs. 7.8% in the experimental sample) contributed to higher percentages of the microbiota in the experimental sample than in the control sample. The significant elevation of Bacteroidetes in the intestinal community of the starved seabass sample is in agreement with some other studies [2, 34, 35] on dietary shifts. For example, in mice [34], fasting was associated with a significant increase in the proportional representation of the Bacteroidetes [from 20.6% (fed) to 42.3% (fasted)]. Bacteroides with a much larger genome size (e.g., Bacteroides fragilis Strain NCTC9343: 5,205,140 bp) are normally mutualistic in the animal gastrointestinal flora. A large part of the proteins made by the Bacteroides genome are able ...
AM-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and Mycoplasma pneumoniae. AM-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against Staphylococcus aureus including methicillin-resistant strains, Staphylococcus epidermidis, Streptococcus pneumoniae, and Enterococcus faecalis; its MICs for 90% of strains tested were 0.10 to 0.78 micrograms/ml. The activity of AM-1155 was comparable to that of ciprofloxacin against members of the family Enterobacteriaceae, Branhamella catarrhalis, Haemophilus influenzae, and Neisseria gonorrhoeae, but was fourfold less than that of ciprofloxacin against Pseudomonas aeruginosa. Against Xanthomonas maltophilia, Acinetobacter calcoaceticus, and Campylobacter jejuni, AM-1155 was two- to fourfold more active than ciprofloxacin. At a concentration of 1.56 micrograms/ml, AM-1155 inhibited 90% of Bacteroides fragilis strains tested; its activity was 8- to 10-fold higher than those ...
The immune system protects against infection and disease, but there are numerous examples where it has switched sides, as in autoimmune diseases and in cancer. Cells become cancerous when they acquire genetic mutations that enable them to evade the regular controls on growth. In addition, stomach (Helicobacter pylori) and liver (hepatitis viruses B and C) cancers have been associated with infection-induced inflammation, which involves immune cells, such as macrophages, and inflammation signaling molecules that can promote tumorigenesis. Research on the mechanisms of inflammation-induced carcinogenesis has focused mainly on the innate branch of the immune system, primarily the NF-κB intracellular signaling pathway.. Colorectal cancer has been linked to inflammation of the colon (colitis), which can be caused by bacterial infections. Wu et al. have found that the human bacterium enterotoxigenic Bacteroides fragilis (ETBF), which induces colitis, can promote colon cancer in a mouse model of the ...
The results of the study demonstrated a decrease in the sensitivity of bacteria in the B.fragilis group to modern anti-anaerobic drugs: cefoxitin, clindamycin, moxifloxacin, imipenem, piperacillin / tazobactam and metronidazole.
Akhi, Mohammad Taghi and Jedari Seifi, Sirus and Asgharzadeh, Mohammad and Ahangarzadeh Rezaee, Mohammad and Abdoli Oskuei, Shahram and Pirzadeh, Tahereh and Memar, Mohammad Yousef and Alizadeh, Naser and Seifi Yarijan Sofla, Hasan (2016) Role of Enterotoxigenic Bacteroides fragilis in Children Less Than 5 Years of Age with Diarrhea in Tabriz, Iran. Jundishapur Journal of Microbiology, Inpres (Inpres). ISSN 2008-3645 Akhi, Mohammad Taghi and Ghotaslou, Reza and Beheshtirouy, Samad and Asgharzadeh, Mohammad and Pirzadeh, Tahereh and Asghari, Babak and Alizadeh, Naser and Toloue Ostadgavahi, Ali and Sorayaei Somesaraei, Vida and Memar, Mohammad Yousef (2015) Antibiotic Susceptibility Pattern of Aerobic and Anaerobic Bacteria Isolated From Surgical Site Infection of Hospitalized Patients. Jundishapur Journal of Microbiology, 8 (7). ISSN 2008-3645 Akhi, Mohammad Taghi and Ghotaslou, Reza and Beheshtirouy, Samad and Asgharzadeh, Mohammad and Pirzadeh, Tahereh and Asghari, Babak and Alizadeh, Naser ...
Work in the Cynthia Sears Laboratory focuses on the bacterial contributions to the development of human colon cancer and the impact of the microbiome on other cancers and the therapy of cancer. The current work involves mouse and human studies to define how enterotoxigenic Bacteroides fragilis, pks+ Escherichia coli, Fusobacterium nucleatum, biofilms and the colonic microbiota induce chronic colonic inflammation and colon cancer. Prospective human studies of the microbiome and biofilms in screening colonoscopy are in progress as are studies to determine if and how the microbiome impacts the response of individuals with cancer to immunotherapy and other cancer therapies ...
Akhi, Mohammad Taghi and Jedari Seifi, Sirus and Asgharzadeh, Mohammad and Ahangarzadeh Rezaee, Mohammad and Abdoli Oskuei, Shahram and Pirzadeh, Tahereh and Memar, Mohammad Yousef and Alizadeh, Naser and Seifi Yarijan Sofla, Hasan (2016) Role of Enterotoxigenic Bacteroides fragilis in Children Less Than 5 Years of Age with Diarrhea in Tabriz, Iran. Jundishapur Journal of Microbiology, Inpres (Inpres). ISSN 2008-3645 ...
The karyotypes of diploid P. fragilis subsp. villosus (2n = 2x = 14) and tetraploid subsp. secaliformis (2n = 4x = 28) were studied by Giemsa C- and N-banding, and AgNO3 staining and compared with the karyotype of subsp. fragilis (2x). The complements of subsp. villosus and subsp. fragilis were similar, with 8 metacentric and 6 SAT-chromosomes, one metacentric and two submetacentric pairs, with small to minute, polymorphic, heterochromatic satellites. The complement of subsp. secaliformis on the whole agreed with a doubling of the complement of diploid P. fragilis, suggesting autopolyploidy. Only the presence of 12 nucleoli in interphases identified 6 SAT-chromosome pairs. In subsp. villosus one or two extra micronucleoli indicated a chromosome pair with very low nucleolus-forming activity, bringing the number of SAT-chromosome pairs to 4. This number may be a characteristic of Psathyrostachys. Besides very small, inconsistently observed bands, the C-banding pattern consisted of 0-3 small bands ...
Synonyms for Bacillus fragilis in Free Thesaurus. Antonyms for Bacillus fragilis. 1 synonym for bacillus: B. What are synonyms for Bacillus fragilis?
Our laboratories are particularly well placed to conduct this interdisciplinary study. The PI, Dr Abratt at the University of cape Town, has specialised anaerobic growth facilities available, and a proven track record of research in the areas of anaerobic microbiology and molecular genetics, particularly of the Bacteroides nitrogen metabolism.. In addition, we have established the capability to determine protein structure in Africa for the first time through a grant by the Carnegie Corporation of New York to B.T. Sewell (co-investigator). This grant has enabled the creation of the joint UCT/UWC Masters programme in Structural Biology and has, in particular led to the establishment of a facility for protein X-ray crystallography at the University of the Western Cape and the establishment of a protein NMR facility at the University of Stellenbosch. Six masters students have been recruited to the programme in its first year (2003). Each student cohort will take two years, including a year of ...
Introduction. Most peritoneal infections are caused by gram-negative and anaerobic bacteria, specially Escherichia coli and Bacteroides fragilis,. Escherichia coli predominates during the acute phase of the process, whereas in the chronic phase, when abscess formation occurs, the predominance is of anaerobic bacteria, mainly Bacteroides fragilis and enterococci, which act synergistically1,2. The association between sepsis and peritonitis has not been fully clarified. During the acute phase, after invasion by gram-negative bacteria, the organism recognizes lipopolysaccharide (LPS) and its major molecule, lipid A, as signals of bacterial penetration. Lipopolysaccharide binding protein (LBP) transfers LPS to CD14 on the surface of monocytes, macrophages and neutrophils3. This phenomenon induces the production and release of mediators, such as tumor necrosis factor α (TNF-α) and interleukins 1, 6 and 8, generating the amplification of the LPS signal in plasma and its transmission to other cells ...
TY - JOUR. T1 - Twenty-eight divergent polysaccharide loci specifying within and amongst strain capsule diversity in three strains of Bacteroides fragilis. AU - Patrick,S.. AU - Blakely,G.W.. AU - Houston,S.. AU - Moore,J.. AU - Abratt,V.R.. AU - dos Santos,Marcelo Bertalan Quintanilha. AU - Cedeño-Tárraga,A.M.. AU - Corton,N.. AU - Corton,C.. AU - Bignell,A.. AU - Barron,A.. AU - Clark,L.. AU - Bentley,S.D.. AU - Parkhill,J.. PY - 2010. Y1 - 2010. N2 - Comparison of the complete genome sequence of Bacteroides fragilis 638R originally isolated in the USA, was made with two previously sequenced strains isolated in the UK (NCTC 9343) and Japan (YCH46). The presence of 10 loci containing genes associated with polysaccharide biosynthesis, each including a putative Wzx flippase and Wzy polymerase, was confirmed in all three strains, despite a lack of cross-reactivity between NCTC 9343 and 638R surface polysaccharide-specific antibodies by immunolabelling and microscopy. Genomic comparisons revealed ...
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA ge
Dynamics of the metallo-beta-lactamase from Bacteroides fragilis in the presence and absence of a tight-binding inhibitor.: A significant determinant for the br
Avila-Campos, Mario Julio et al. Antimicrobial resistance and plasmid detection in strains of the Bacteroides fragilis group. Rev. Inst. Med. trop. S. Paulo, Feb 1993, vol.35, no.1, p.107-110. ISSN 0036- ...
To study the development of these immune cells in the context of gut microbes, researchers monocolonized germ-free mice with a model human commensal, Bacteroides fragilis, and demonstrated that this single species of bacteria could restore the development of PLZF+ innate and innate-like lymphocytes in the thymus of infant mice.. In further proof-of-concept studies, they showed that a mutant B. fragilis lacking expression of Polysaccharide A (PSA) was unable to do the same, suggesting that specific microbial antigens could regulate this early life developmental process.. A similar deficit in these cells was observed in mutant mice that lacked the expression of Toll like receptor 2, a receptor that recognizes bacteria and bacterial components, including B. fragilis PSA, to initiate host protective immune responses.. To understand how this microbial message was delivered to developing thymic cells, Jains group used a novel mouse model to track the migration of cells from the colon to the thymus. ...
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S1, a new chromogenic cephalosporin (International BioClinical, Inc., Portland, Oreg.), was used to detect beta-lactamase production among a variety of commonly encountered bacteria in a four-center collaborative study. Results of an S1 disk assay were compared with those obtained by a nitrocefin-based disk procedure (Cefinase; Becton-Dickinson Microbiology Systems, Cockeysville, Md.), with repetitive testing of five quality control organisms and with individual tests of recent clinical isolates of Neisseria gonorrhoeae (162 strains), Haemophilus influenzae (162 strains), Moraxella catarrhalis (155 strains), Staphylococcus aureus (161 strains), and Bacteroides fragilis (164 strains). The performances of the two beta-lactamase disk assays were comparable for the first three species cited above. However, the S1 assay appeared to be a more sensitive procedure than the Cefinase assay when applied to S. aureus and B. fragilis, with respect to both total numbers of positive results and length of time to a
The enumeration of phages infecting host-specific strains of Bacteroides has been widely recognised as an effective and low-cost method of microbial source tracking (MST). A recently described human-specific Bacteroides host strain (GB-124) has been shown to detect bacteriophages exclusively in human-impacted waters and is emerging as a useful MST tool. However, a better understanding of the morphology and ecological behaviour of the phages, especially in wastewater disinfection processes, is now required in order to validate their role as MST markers. Bacteriophages infecting Bacteroides fragilis GB-124 (n = 21) were isolated from wastewater effluent and irradiated using laboratory-based UV-C (254 nm) collimated beam experiments. Bacteriophages were found to be both a morphologically and ecologically homogeneous group, with all specimens showing highly similar first order log-linear inactivation profiles (mean fluence required to inactivate phages by 4-log10 was 36 mJ/cm2). These findings ...
1) Rasmussen BA, et al. (1990) Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.. Antimicrob Agents Chemother 34(8):1590-2 PubMed: 2121094 ...
Azithromycin (Zithromax) est un antibiotique macrolide semi-synthétique chimiquement dérivé de lérythromycine qui est actif contre un grand nombre de types de micro-organismes de gram positif et de gram négatif tels que le genre Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro, il sest caractérisé par une activité contre Legionella pneumophila, Mycoplasma pneumoiae Ø hominis, pylores de Helicobacter, Toxoplasma gondii, Ureaplasma urealiticum. ...
As the appreciation for anaerobic bacteriology has grown, it has become apparent that the isolation of Bacteroides species from clinical isolates is a significant finding. Studies by Redondo et al. (15) and Nguyen et al. (14) have linked Bacteroides bacteremia to higher mortality rates, which can be further affected by the use of inactive antimicrobials in the treatment regimen.. The class of 5-nitroimidazole drugs (metronidazole, tinidizole, and ornidizole) exerts antimicrobial actions via inhibition of DNA synthesis (19). This action requires intracellular reduction of the nitro group of these prodrugs to produce reactive radical species. Although the 5-nitroimidazole drugs were introduced into clinical practice in 1960, resistance of B. fragilis to these drugs was not described until 1978 (10) and remains rare (1, 20-22). The first metronidazole-resistant Bacteroides isolates were found to have altered end products of glucose metabolism that conferred distinctive biochemical and growth ...
Bacillus cereus from the Bacillus cereus group species, which consist of: Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus weihenstephanensis, Bacillus mycoides and Bacillus pseudomycoides is one of the most frequently isolated bacterial foodborne pathogens. Growth of B. cereus results in production of several highly active toxins therefore, consumption of food containing 105-106 bacteria (spores)/g or toxins, is sufficient to cause emetic and diarrhoeal syndromes. The most common source of this bacterium is milk and mixed food products that include milk powder, thus is of particular concern in the baby formula industry. In this study 138 strains of B. cereus group spp. were characterized based on their phenotypic and genotypic features. The study developed unique DNA primers for use in PCR and these were then tested via real-time PCR (RT-PCR): (i) the motB gene encoding the flagellar motor protein MotB was used as a PCR primer target. (ii) New primers and probes, targeting ...
Probiotic yogurts and pills promise better health, but the bacteria we ingest find a complex and challenging environment when they enter our gut. Food sources shift with every meal we eat, and the threat of pathogens is ever-present. In this competitive landscape, theres a clear winner already on the field: the genus Bacteroides makes up 30 percent of the bacteria in the human gut, the single most abundant genus.. Bacteroides are a successful bacterium in our gut microbiome. By offering metabolic capabilities that humans dont have, it helps us to obtain important nutrients. So why are Bacteroides so resilient? If we can understand their success, it will ultimately allow us to design probiotic formulations for human health and aid patients with an imbalance of gut bacteria, said Blanca Barquera, an associate professor of biological sciences at Rensselaer Polytechnic Institute and lead researcher on a new project investigating Bacteroides.. With support from a four-year $3 million grant from ...
Define Bacteroides salivosus. Bacteroides salivosus synonyms, Bacteroides salivosus pronunciation, Bacteroides salivosus translation, English dictionary definition of Bacteroides salivosus. Noun 1. Bacteroides - type genus of Bacteroidaceae; genus of Gram-negative rodlike anaerobic bacteria producing no endospores and no pigment and living in...
cannot utilise lactose. One possible approach to get around this problem would be to clone the genes responsible for lactose utilisation from a lactose utilising yeast (in this case the genes LAC4 and LAC12 from K.marxianus var.fragilis) into S.cerevisiae. As a preliminary step in the cloning LAC4 and LAC12 into S.cerevisiae a genomic library of an industrial strain of the lactose-utilising yeast, K.marxianus var. fragilis was constructed. Partially Sau3AI digested, size selected fragments of K.marxianus var.fragilis DNA were cloned into the Saccharomyces-Eschericia coli shuttle vector, YEp 13 and the resulting recombinant plasmids were transformed into E.coli DH5α. The resulting library was found to contain approximately 15 000 recombinant plasmid bearing clones with an average insert size of 7.7kb. This library thus contains approximately 116 OOOkb of DNA which is equivalent to approximately 8 times the genome size of K.marxianus var. fragilis. The two genes of interest, LAC4 (which encodes ...
Dezesseis eqüinos adultos foram distribuídos aleatoriamente em 4 grupos (GI, GII, GIII e GIV) constituídos por quatro animais, recebendo cada grupo o seguinte inóculo por via intraperitoneal: GI (100 X 10(7) unidades formadoras de colônia (UFC) de Escherichia coli diluídos em 500 ml de solução salina 0,9% estéril); GII (100 X 10(7) UFC de Bacteroides fragilis diluídos em 500 ml de solução salina 0,9% estéril); GIII (100 X 10(7) UFC de Escherichia coli associados a 100 X 10(7) UFC de Bacteroides fragilis diluídos em 500 ml de solução salina 0,9% estéril); GIV (testemunho - 500 ml de solução salina 0,9% estéril). Leucopenia ocorreu em todos os animais inoculados com bactérias, nas primeiras seis horas após as inoculações. Posteriormente a este período, verificou-se em alguns eqüinos inoculados leucocitose. Os eqüinos inoculados com culturas puras de E. coli ou B. fragilis apresentaram peritonites brandas e autolimitantes, enquanto os inoculados com a associação destas ...
TY - JOUR. T1 - An anaerobic bacterium, Bacteroides thetaiotaomicron, uses a consortium of enzymes to scavenge hydrogen peroxide. AU - Mishra, Surabhi. AU - Imlay, James A.. PY - 2013/12. Y1 - 2013/12. N2 - Summary: Obligate anaerobes are periodically exposed to oxygen, and it has been conjectured that on such occasions their low-potential biochemistry will predispose them to rapid ROS formation. We sought to identify scavenging enzymes that might protect the anaerobe Bacteroides thetaiotaomicron from the H2O2 that would be formed. Genetic analysis of eight candidate enzymes revealed that four of these scavenge H2O2in vivo: rubrerythrins 1 and 2, AhpCF, and catalase E. The rubrerythrins served as key peroxidases under anoxic conditions. However, they quickly lost activity upon aeration, and AhpCF and catalase were induced to compensate. The AhpCF is an NADH peroxidase that effectively degraded low micromolar levels of H2O2, while the catalytic cycle of catalase enabled it to quickly degrade ...
Bacteroides spp. are dominant components of the phylum Bacteroidetes in the gut microbiota and prosper in glycan enriched environments. However, knowledge of the machinery of specific species isolated from humans (like Bacteroides uniformis) contributing to the utilization of dietary and endogenous sources of glycans and their byproducts is limited. We have used the cutting-edge nanopore-based technology to sequence the genome of B. uniformis CECT 7771, a human symbiont with a proven pre-clinical efficacy on metabolic and immune dysfunctions in obesity animal models. We have also used massive sequencing approaches to distinguish the genome expression patterns in response to carbon sources of different complexity during growth. At genome-wide level, our analyses globally demonstrate that B. uniformis strains exhibit an expanded glycolytic capability when compared with other Bacteroides species. Moreover, by studying the growth and whole-genome expression of B. uniformis CECT 7771 in response to different
TY - JOUR. T1 - The use of parenteral clindamycin in the treatment of obstetric gynecologic patients with severe infections. A comparison of a clindamycin kanamycin combination with penicillin kanamycin. AU - Ledger, W. J.. AU - Kriewall, T. J.. AU - Sweet, Richard L. AU - Fekety, F. R.. PY - 1974. Y1 - 1974. N2 - Using a system of random selection in patients with severe obstetric gynecologic infections, parenteral penicillin kanamycin was given to 23 women and clindamycin kanamycin was given to 21. Therapeutic response to the two antibiotic regimens was similar; no significant differences were noted in either the posttreatment fever index or the numbers of women requiring other therapy. There was a failure of response to the penicillin kanamycin regimen in those women with Bacteroides fragilis infection and to the clindamycin kanamycin regimen in some of the women with an Enterococcus infection. The implications of these findings for the future selection of antibiotics for patients with severe ...
Bacteroides é un xénero de bacterias gramnegativas, con forma de bacilo. As especies de Bacteroides non forman esporas, son anaeróbicas, e hai especies móbiles e inmóbiles.[1] A composición de bases do seu ADN ten un contido GC do 40-48%. Bacteroides ten esfingolípidos nas súas membranas, o que é infrecuente nas bacterias. Tamén conteñen ácido meso-diaminopimélico na capa de peptidoglicano da súa parede celular. As especies de Bacteroides son normalmente mutualistas, e constitúen a parte principal da flora intestinal dos mamíferos,[2] onde xogan un papel fundamental no procesamento de moléculas complexas noutras máis simples no intestino do hóspede.[3][4][5] Nas feces humanas informouse dunha cantidade de 1010-1011 células por gramo.[6] Aínda que poden utilizar azucres simples cando dispoñen deles, as principais fontes de enerxía para Bacteroides no intestino son os glicanos (polisacáridos) complexos derivados do hóspede ou de plantas.[7] Certos estudos indican que a ...
Rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. The enzymes required for these hydrolyses, namely alpha-rhamnosidase and beta-galactosidase, were produced by some strains of Bacteroides distasonis; other strains, however, synthesized beta-glucosidase. The last-named enzyme was also elaborated by Bacteroides uniformis and Bacteroides ovatus. All the enzymes were produced constitutively. A cell-free extract of B. distasonis containing beta-glucosidase displayed an enzymic activity of 1 mumol/10 min per 10 mg of protein. ...
Bacteroides is a genus of Gram-negative, rod-shaped bacteria. Bacteroides species are non-endospore-forming, anaerobes, and may either be motile or non-motile, depending on the species.[1] The DNA base composition is 40-48% GC. Unusual in bacterial organisms, Bacteroides membranes contain sphingolipids. They also contain meso-diaminopimelic acid in their peptidoglycan layer. Bacteroides are normally commensal, making up the most substantial portion of the mammalian gastrointestinal flora,[2] where they play a fundamental role in processing of complex molecules to simpler ones in the host intestine. As many as 1010-1011 cells per gram of human feces have been reported.[3] They can use simple sugars when available, but the main source of energy is polysaccharides from plant sources. ...
A molecular method, termed hierarchical oligonucleotide primer extension (HOPE), was used to determine the relative abundances of predominant Bacteroides spp. present in fecal microbiota and wastewaters. For this analysis, genomic DNA in feces of healthy human adults, bovines, and swine and in wastewaters was extracted and total bacterial 16S rRNA genes were PCR amplified and used as the DNA templates for HOPE. Nineteen oligonucleotide primers were designed to detect 14 Bacteroides spp. at different hierarchical levels (domain, order, cluster, and species) and were arranged into and used in six multiplex HOPE reaction mixtures. Results showed that species like B. vulgatus, B. thetaiotaomicron, B. caccae, B. uniformis, B. fragilis, B. eggerthii, and B. massiliensis could be individually detected in human feces at abundances corresponding to as little as 0.1% of PCR-amplified 16S rRNA genes. Minor species like B. pyogenes, B. salyersiae, and B. nordii were detected only collectively using a primer ...
Humans excrete between 50 to 100 mg of volatile phenols per day, predominantly in the form of 4-cresol and phenol (mainly as glucuronide and sulfate conjugates) with lower amounts of 4-ethylphenol (20). The production of cresols from tyrosine in mammals has been attributed to various species of Clostridium, Bifidobacterium, and Bacteroides fragilis. Other bacteria such as E. coli are associated with phenol production. Altered levels of 4-cresol metabolites in human urine have been associated with diverse physiological and pathological conditions from weight loss to inflammatory bowel disease. These conditions are also associated with altered microbiota composition, namely a reduction in the diversity of the microbiota because of loss of Lactobacillus and Bacteroides species in the case of inflammatory bowel disease (21) and differences in the ratio of the Firmicutes to Bacteroidetes species in the case of weight loss (22).. Dietary fiber (complex carbohydrates) can be digested and subsequently ...
Bacteroides barnesiae has a size of 0.5-1.4 micrometer wide and 0.8-10.6 micrometer long that is completely anaerobic and grows at temperature of about 37C which does not require oxygen to survive. It has a cellular fatty acids including C15:0 and iso-C15:0 and in cultured media appears to be circular, raised and grayish-white colonies. Bacteroides barnesiae is predominantly found in gastrointestinal tract of humans as well as in vertebrate animals where bile is present. It gives benefits to the host if this bacterium strictly retained in the gut by hindering pathogenic microorganisms from forming colonies in the intestines. Bacteroides barnesiae play a basic role in the breakdown of polysaccharides a complex molecule into small molecules that is used by the bacterium as well as the host in order to utilize the nitrogenous substances and biotransformation of steroids and bile acids. Scientific classification: ...
Microbiota. The development and function of the intestinal immune system is influenced by the gut microbiota, including bacteria with the capacity to induce specific lineage differentiation in immune cells. Germ-free mice have an underdeveloped intestinal immune system, signifying a role for MAMPs in its establishment (21). Some commensal bacteria such as segmented filamentous bacteria (SFBs) preferentially induce a Th17 response in T cells (22, 23). Other commensals or their products guide an IL-10 Treg response. The capsular polysaccharide-A moiety on Bacteroides fragilis promotes expansion of Foxp3+ Tregs through a TLR2/MyD88 mechanism (24), while Clostridium species can induce Treg formation independent of MyD88, potentially through TGF-β (25). Bacterial metabolites like short-chain fatty acids (SCFA) produced by Akkermansia muciniphila and Faecalibacterium prausnitzii also influence immunity. For instance, butyrate from these organisms increases the differentiation of Tregs in the colon ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
This HMM describes a set of proteins related to but longer than DNA-binding protein HU. Its distinctive domain architecture compared to HU and related histone-like DNA-binding proteins justifies the designation as superfamily. Members include, so far, one from Bacteroides fragilis, a gut bacterium, and ten from Porphyromonas gingivalis, an oral anaerobe ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Zithromax is a semi-synthetic macrolide antibiotic chemically related to erythromycin which is effective against majority of species of gram positive and gram negative microorganisms such as genus Staphylococcus; S. aureus, S. pneumoiane, S. pyogenes, S. agalactiae, Haemophilus influenzae and parainfluenzae, Moraxela catarrhalis, Bacteroides fragilis, Escherichia coli, Bordetella ssp., Borrelia burgdorferi, Haemophilus ducreui, Nisseria gonorrhoeae Ø Chlamidia trachomati. In vitro it showed activity against Legionella pneumophila, Mycoplasma pneumoia Ø hominis, Helicobacter pylori, Toxoplasma gondii, Ureaplasma urealiticum. As a Macrolide antibiotic Zithromax inhibits bacterial protein synthesis and prevents bacteria from growth and propagation. It is used to treat infections of upper and low respiratory organs (tonsillitis, otitis, sinusitis, pneumonia), urogenital infections (urethritis, prostatitis, cervicitis, adnexitis caused by chlamydia, gonorrhea, early syphilis), intestinal ...
Cefpodoxime is an oral, third-generation cephalosporin antibiotic. It is active against most Gram-positive and Gram-negative organisms. Notable exceptions include Pseudomonas aeruginosa, Enterococcus, and Bacteroides fragilis. Currently, it is only marketed as generic preparations in the USA, according to the FDA Orange Book. It is commonly used to treat acute otitis media, pharyngitis, sinusitis, and gonorrhea. It also finds use as oral continuation therapy when intravenous cephalosporins (such as ceftriaxone) are no longer necessary for continued treatment.. Zoetis markets cefpodoxime proxetil under the trade name Simplicef for veterinary use. The dose range in dogs is 5-10 mg/kg body weight, administered orally, once a day.. Finecure, India markets the products under trade name Cefpo.. Cefpodoxime inhibits cell wall synthesis by inhibiting the final transpeptidation step of peptidoglycan synthesis in cell walls. It has well established pharmacokinetic profile with absorption of 50%. It is ...
Alpod-CV from Altar LS, Cefpodoxime - Cafage-Cl-P to Cefage O | Cefpodoxime (marketed as the prodrug cefpodoxime proxetil by Pharmacia & Upjohn under the trade name Vantin and under the name Orelox by Sanofi-Aventis), is an oral third generation cephalosporin. Use of Alpod-CV from Altar LS, Pregnancy, lactation in childrens and special precautions for Alpod-CV from Altar LS, prices of Alpod-CV from Altar LS . It is active against most Gram positive and Gram negative organisms. Notable exceptions include Pseudomonas aeruginosa, Enterococcus, and Bacteroides fragilis. It is commonly used to treat acute otitis media, pharyngitis, and sinusitis. It also finds use as oral continuation therapy when intravenous cephalosporins (such as ceftriaxone) are no longer necessary for continued treatment., drugsupdate.com - Indias leading online platform for Doctors and health care professionals. Updates on Drugs, news, journals, 1000s of videos, national and international events, product-launches and much more..
Asthma is an increasingly common chronic inflammatory airway disorder with phenotypic heterogeneity driven by age, gender, atopy, lung function, lifestyle, body mass indexes, and the underlying cellular and molecular pathways. In asthma, T helper (Th) cells drive inflammatory cascades via cytokine/inflammatory mediators and recruit leukocytes into the airway. While treatments are available, such as inhaled corticosteroids, individuals with severe asthma are often steroid resistant and have a poor quality of life. It is therefore important to develop our understanding of the underlying T cell driven mechanisms leading to pulmonary inflammation such that novel therapies may be developed. Previous work from our lab has demonstrated the importance of commensal microbiota in the development and maintenance of peripheral homeostasis. Exposure to bacterial polysaccharides, such as PSA from Bacteroides fragilis, inhibits Th1 and Th2 activity in murine models by activation of CD4+ FoxP3− CD45Rblo ...
While doing geological research in the Namibian desert, scientists believe they uncovered proof of the first animal to have existed on Earth. The sponge-like fossil,…. ...
After several months the gut microbiomes between the two set of infants became more similar with one striking difference - the c-section babies had significantly lower levels of Bacteroides, a strain vital to human health.. Bacteroides are a key strain when it comes to health challenges modern societies face. A number of studies have shown Bacteroides levels are lower in people with obesity. Studies in both mice and humans show that when gut bacteria from thin subjects are transplanted into the colons of obese subjects, most subjects lose weight.. Bacteroides has also been linked with preventing anxiety, and boosting and regulating immunity to prevent inflammatory disorders. This may explain why people who were born via c-section are at increased risk for obesity and asthma.. The study is part of a larger Baby Biome study that is following thousands of newborns through childhood. ...
Bacteroides, Bacterial Identification and Bacteria Treatment are considered an interesting alternative to the traditional indicator organisms such as E.coli
7.0 7.1 Bisby F.A., Roskov Y.R., Orrell T.M., Nicolson D., Paglinawan L.E., Bailly N., Kirk P.M., Bourgoin T., Baillargeon G., Ouvrard D. (red.) (2011). Species 2000 & ITIS Catalogue of Life: 2011 Annual Checklist.. Species 2000: Reading, UK. Ginkuhà 24 september 2012. ...
Members of the order Siphonophorae include A-form hydroids. Life cycle: The zygote develops into planula inside the gonophore and is later released as an actinula which metamorphoses into polyp stage (Ref. 833). ...
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Biological classification is how biologists group organisms. The classification has its root in the work of Aristotle who invented a multi-ranked system. A great influence was Carolus Linnaeus, who popularized the idea of binomial nomenclature using a two-part name indicating the genus, and the species. The human species is named Homo sapiens. Names of species are often printed in italics, although there is no obligation to do so (this also goes for names of genera, etc., etc.) Biological classification is also known as taxonomy. It is a science, and like most sciences has evolved over time. At various times different principles were adopted, and it is not rare for different scientists to use different methods. Since the early 20th century, groupings are supposed to fit the Darwinian principle of common descent. These days, molecular evolution studies, which use DNA sequence analysis as data, are popular. This is often called phylogenetics, a branch or form of cladism. This approach creates an ...
[좌측부터 체질 1형 2형 3형] 1) 박테로이데스(Bacteroides) : 탄수화물을 분해하고 비타민 B2, B5, C, H를 만드는 것이 주된 기능이다. 박테리오데스 세균이 많은 속에 속하는 피실험자들 중에는 비만을 겪는 사...
Bacteroides spp., including B. fragilis Enterobacter spp. Escherichia coli Haemophilus influenzae Klebsiella species Morganella ...
m Bacteroides fragilis‎; 11:49 . . (+24)‎ . . ‎. مصعب. (talk , contribs)‎ (→‎External links: clean up using AWB) ... m Bacteroides fragilis‎; 16:58 . . (+29)‎ . . ‎. Kku. (talk , contribs)‎ (link antibiotic resistance using Find link) ...
Bacteroides fragilis). Fecal peritonitis results from the presence of faeces in the peritoneal cavity. It can result from ...
An example of this is Appendicitis, which is caused by Bacteroides fragilis and Escherichia coli. The second is an iatrogenic ...
Erridge, C; Pridmore, A; Eley, A; Stewart, J; Poxton, IR (2004). "Lipopolysaccharides of Bacteroides fragilis, Chlamydia ...
Bacteroides fragilis värvituna Grami järgi. Taksonoomia Riik Bakterid Bacteria. Hõimkond Bacteroidetes. Bacteroidetes on ...
The Fischbach lab discovered that the gut commensal Bacteroides fragilis produces the immune modulatory sphingolipid alpha- ...
One of the most important clinically is Bacteroides fragilis. Bacteroides melaninogenicus has recently been reclassified and ... Bacteroides species also benefit their host by excluding potential pathogens from colonizing the gut. Some species (B. fragilis ... Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, ... Bacteroides such as Bacteroides thetaiotaomicron converts these sugars to fermentation products which are beneficial to humans ...
... and Bacteroides fragilis strains having various resistance phenotypes". Antimicrobial Agents and Chemotherapy. 55 (5): 2390-4. ...
Bacteroides fragilis was the first Bacteroides species isolated in 1898 as a human pathogen linked to appendicitis among other ... Some Bacteroides spp. have a potential to utilize urea as a nitrogen source. Other important functions of Bacteroides spp. ... as non-digestible oligosaccharides in mother milk support the growth of both Bacteroides and Bifidobacterium spp. Bacteroides ... Some Bacteroides spp. and Prevotella spp. can degrade complex plant polysaccharides such as starch, cellulose, xylans, and ...
Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of ... CepA (Chromosomal cephalosporinase from Bacteroides fragilis belonging to Ambler class A) is an endogenous cephalosporinase A ... described in Bacteroides fragilis. This beta- lactamase is ubiquitous, but frequently inactive. CepA is encoded by the cepA ... Most beta-lactamases identified in Bacteroides, Prevotella, and Capnocytophaga belong to the Ambler class A. Several beta- ...
Bacteroides fragilis and Peptostreptococcus species. Imipenem/cilastatin was approved for use in the United States in 1985. ...
Unha das especies do xénero máis importantes clinicalmente é Bacteroides fragilis. Bacteroides melaninogenicus foi ... Bacteroides é un xénero de bacterias gramnegativas, con forma de bacilo. As especies de Bacteroides non forman esporas, son ... Non se debe confundir o xénero Bacteroides co tipo morfolóxico de bacterias fixadoras de N (rizobios) chamado bacteroide.. ... "Bacteroides Infection: Overview - eMedicine". Arquivado dende o orixinal o 22 de decembro de 2008. Consultado o 2008-12-11.. ...
... which include Bacteroides fragilis and Bacteroides melaninogenicus, Peptostreptococcus spp., Streptococcus microaerophile, ...
Bacteroides fragilis. *Tannerella forsythia. *Capnocytophaga canimorsus. *Porphyromonas gingivalis. *Prevotella intermedia. ...
Bacteroides fragilis. *Tannerella forsythia. *Capnocytophaga canimorsus. *Porphyromonas gingivalis. *Prevotella intermedia. ...
Dientamoeba fragilis intestines stool up to 10% in industrialized countries ingesting water or food contaminated with feces ...
Bacteroides species are normally mutualistic bacteria, making up a substantial portion of the mammalian gastrointestinal flora. ... 20] Some species like B. fragilis for example are opportunistic pathogens, causing infections of the peritoneal cavity inhibit ...
... and Bacteroides species can prolong the ulcerative process. Fungal causes include Coccidioides immitis (valley fever), ... vesicles and bullae which form on the mucous membranes of the oral cavity tend to be fragile and quickly break down to leave ... making the lining more fragile and easily breached.[19]:7 Stomatitis is a general term meaning inflammation within the mouth, ...
Con diferenza, a clase Bacteroidia é a mellor estudada deste filo, e inclúe os xéneros Bacteroides (un organismo abundante nas ... Membros do xénero Bacteroides son patóxenos oportunistas. Os membros das outras dúas clases raramente son patóxenos para os ...
Ionfhabhtú Bacteroides. Géineas Bacteroides. Balantidiasis. Balantidium coli. Ionfhabhtú Baylisascaris. Géineas Baylisascaris. ... Dientamoeba fragilis. Diphtheria. Corynebacterium diphtheriae. Diphyllobothriasis. Diphyllobothrium. Dracunculiasis. ...
We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other ... Bacteroides fragilis enterotoxin gene sequences in patients with inflammatory bowel disease Emerg Infect Dis. Mar-Apr 2000;6(2 ... We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other ... none of those with inactive disease had specimens positive for enterotoxigenic Bacteroides fragilis gene sequences. ...
Bacteroides fragilis ATCC ® 25285™ Designation: VPI 2553 TypeStrain=True Application: Media testing Quality control strain ... Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers (ATCC® 25285™) Strain Designations: VPI 2553 [EN-2; NCTC 9343] ... Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides ... Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers (ATCC® 25285D-5™) Add to ...
Bacteroides fragilis 3_1_12 supercont1.5 genomic scaffold, whole genome shotgun ... Bacteroides fragilis 3_1_12 supercont1.5 ... Bacteroides fragilis 3_1_12 supercont1.5 genomic scaffold, whole genome shotgun sequence. GenBank: EQ973217.1 ...
Cellular immunity to Bacteroides fragilis capsular polysaccharide.. M E Shapiro, A B Onderdonk, D L Kasper, R W Finberg ... The polysaccharide capsule of Bacteroides fragilis has been shown to be important in the virulence of the organism. The ... Immunization of C57BL/10J mice with the CP over 5 wk prevents abscess formation when the mice are challenged with B. fragilis ... capsular polysaccharide (CP) of B. fragilis has been extensively purified. Using a murine model of intraabdominal abscess ...
Characterization of the BatI (Bacteroides aerotolerance) operon in Bacteroides fragilis: isolation of a B. fragilis mutant with ... Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes. D ... The role of BmoR, a MarR Family Regulator, in the survival of Bacteroides fragilis during oxidative stress ... Dps and DpsL Mediate SurvivalIn VitroandIn Vivoduring the Prolonged Oxidative Stress Response in Bacteroides fragilis ...
Bacteroides fragilis str. 2-F-2 #4. Bacteroides fragilis CAG:47. Bacteroides fragilis str. 3774 T13. Bacteroides fragilis str. ... Bacteroides fragilis. Bacteroides cellulosilyticus. Bacteroides sp. 2_1_56FAA. Bacteroides fragilis str. S23L17. ... Bacteroides sp. 1_1_6. Bacteroides ovatus. Bacteroides ovatus CL02T12C04. Bacteroides sp. 2_2_4. Bacteroides xylanisolvens XB1A ... Bacteroides ovatus 3_8_47FAA. Bacteroides thetaiotaomicron. Bacteroides sp. 1_1_14. Bacteroides finegoldii DSM 17565. ...
Taxonomy of the Bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides ... The genetic element flanking the Bacteroides fragilis pathogenicity island (BfPAI) in enterotoxigenic B. fragilis (ETBF) strain ... The Bacteroides fragilis Pathogenicity Island Is Contained in a Putative Novel Conjugative Transposon. Augusto A. Franco ... The Bacteroides fragilis toxin fragilysin disrupts the paracellular barrier of epithelial cells. Infect. Immun. 65:1431-1439. ...
Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli.. T A Russo, J S ... We have cloned the Bacteroides fragilis TAL2480 neuraminidase (NANase) structural gene, nanH, in Escherichia coli. This was ... Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli. ... Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli. ...
This occurs before ETBF-induced mucosal permeability, suggesting that ETBF, likely through B. fragilis toxin and its action on ... Background: Enterotoxigenic Bacteroides fragilis (ETBF), a molecular subclass of the common human commensal, B. fragilis, has ... Stat3 activation in murine colitis induced by enterotoxigenic Bacteroides fragilis Inflamm Bowel Dis. 2014 May;20(5):821-34. ... Methods: C57BL/6 wild-type, C57BL/6, or Rag-1 mice were inoculated with saline, nontoxigenic B. fragilis or ETBF. Histologic ...
... none of those with inactive disease had specimens positive for enterotoxigenic Bacteroides fragilis gene sequences. ... We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other ... Shetab R, Cohen SH, Prindiville TP, Tang YJ, Cantrell M, Rahmani D, Detection of Bacteroides fragilis enterotoxin gene by PCR. ... Sack RB, Myers LL, Almeido-Hill J, Shoop DS, Bradbury WC, Reid R, Enterotoxigenic Bacteroides fragilis: epidemiologic studies ...
cfiA, bacteroides fragilis, imipenem, meropenem, insertion sequence (IS) elements Identifiers. URN: urn:nbn:se:umu:diva-26393 ... The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-beta-lactamase via ... Examination of cfiA-mediated carbapenem resistance in Bacteroides fragilis strains from a European antibiotic susceptibility ... Of 1284 Bacteroides strains collected in Europe in 2000 for antibiotic susceptibility surveillance, 65 isolates displayed ...
N2 - Comparison of the complete genome sequence of Bacteroides fragilis 638R originally isolated in the USA, was made with two ... AB - Comparison of the complete genome sequence of Bacteroides fragilis 638R originally isolated in the USA, was made with two ... abstract = "Comparison of the complete genome sequence of Bacteroides fragilis 638R originally isolated in the USA, was made ... divergent polysaccharide loci specifying within and amongst strain capsule diversity in three strains of Bacteroides fragilis ...
N2 - Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have been ... AB - Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have been ... Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have been ... abstract = "Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have ...
α-Galactosidase 110A from Bacteroides fragilis, Recombinant. α-Galactosidase 110A from Bacteroides fragilis, Recombinant. ...
... fragilis isolated from pregnant women in their 38th week of ... The investigation was performed with 9 strains of B. fragilis ... Biological activity of Bacteroides fragilis].: The investigation was performed with 9 strains of B. ...
Varel, V. H., & Bryant, M. P. (1974). Nutritional features of Bacteroides fragilis subsp. fragilis. Appl Microbiol, 28(2), 251- ... Bacteroides fragilis brain abscess and meningitis. Eur J Pediatr, 155(6), 517-8. PMID: 8789773 () ... Bacteroides fragilis is an opportunistic pathogen, occurring normally in the human digestive tract, but causing severe and ... Bacteroides fragilis is an anaerobic opportunistic pathogen, occurring normally in the human digestive tract, but causing ...
The results of the study demonstrated a decrease in the sensitivity of bacteria in the B.fragilis group to modern anti- ... Antibiotic resistance of anaerobic bacteria of the Bacteroides fragilis group isolated in Europe. The June issue of Clinical ... Among the strains studied, B.fragilis (65%), B.thetaiotaomicron (16%) and B.vulgatus (8% ) predominant other representatives of ... Comparing the data with the results of a similar study carried out in 1992, the sensitivity of bacteria of the B.fragilis group ...
Bacteroides distasonis shows the highest resistance rates among β-lactam antibiotics, whereas Bacteroides vulgatus shows the ... B. fragilis shows the lowest resistance rates for all antibiotics. All strains were susceptible to chloramphenicol and ... Standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of Bacteroides fragilis ... Standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of Bacteroides fragilis ...
Bacteroides references in Barons Medical Microbiology (online at the NCBI bookshelf). Type strain of Bacteroides fragilis at ... Bacteroides fragilis is an obligately anaerobic, Gram-negative, rod-shaped bacterium. It is part of the normal flora of the ... Although the B. fragilis group is the most common species found in clinical specimens, it is the least common Bacteroides ... B. fragilis is the most prevalent organism in the B. fragilis group, accounting for 41% to 78% of the isolates of the group. ...
... The Bacteroides fragilis group consists of species of ... Species identification was confirmed as Bacteroides fragilis ssp. fragilis by biochemical testing, mass spectrometry, and ... Multidrug resistant Bacteroides fragilis recovered from blood and severe leg wounds caused by an improvised explosive device ( ... Bacteroides fragilis are anaerobic bacteria found in the human gastrointestinal tract and often cause intra-abdominal ...
Bacteroides fragilis. Mutation(s): 0 Gene Names: bft-3. EC: 3.4.24.74. ... Enterotoxigenic Bacteroides fragilis is the most frequent disease-causing anaerobe in the intestinal tract of humans and ... Enterotoxigenic Bacteroides fragilis is the most frequent disease-causing anaerobe in the intestinal tract of humans and ... Because no similar protein has been found outside enterotoxigenic B. fragilis, our findings support that fragilysins derived ...
Bacteroides fragilis ATCC ® 25285™ Designation: VPI 2553 TypeStrain=True Application: Media testing Quality control strain ... Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers (ATCC® 25285™) Click here to learn about our Enhanced ... Quantitative Genomic DNA from Bacteroides fragilis (ATCC® 25285DQ™) Add to frozen Specification range: ≥1 x 105 copies/µL. 100 ... Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides ...
Bacteroides fragilis subspecies in clinical isolates. Polk, B. Frank; Kasper, Dennis L.; Polk, B F; Kasper, D L // Annals of ... Bacteroides fragilis group infections in HIV-infected patients. Patey, O.; Breuil, J. // AIDS Patient Care & STDs;Oct97, Vol. ... Details on the bacteroides fragilis group; Reference to studies which were performed in hospitals in France; Results of the ... The Role of Bacteroides fragilis in Pelvic Infections. Osborne, Newton G. // Journal of Gynecologic Surgery;Summer2006, Vol. 22 ...
Bacteroides fragilis NCTC 9343. › Bacteroides fragilis NCTC9343. › Bacteroides fragilis str. NCTC 9343. › Bacteroides fragilis ... Taxonomy - Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) Basket 0 ... Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343). Taxonomy navigation. › Bacteroides fragilis ...
Cysteine and methionine metabolism - Bacteroides fragilis YCH46 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
The symbiont Bacteroides fragilis constitutes a relatively small proportion (up to 1%-2%) of cultured fecal bacteria, but ... The symbiont Bacteroides fragilis constitutes a relatively small proportion (up to 1%-2%) of cultured fecal bacteria, but ... fragilis toxin (BFT). Strains that do not secrete BFT are nontoxigenic B. fragilis (NTBF), and those that do are called ... There are 2 classes of B. fragilis distinguished by their ability to secrete a zinc-dependent metalloprotease toxin, B. ...
This article describes a case of Bacteroides fragilis endocarditis associated with portal and superior mesenteric venous ... Endocarditis due to Bacteroides fragilis is a rare disorder. ... Bacteroides fragilis endocarditis: a case report and review of ... Endocarditis due to Bacteroides fragilis is a rare disorder. This article describes a case of Bacteroides fragilis endocarditis ...
Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis.. Veeranagouda ... Metronidazole is the most commonly used antimicrobial for Bacteroides fragilis infections and is recommended for prophylaxis of ... Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis ... Deficiency of the ferrous iron transporter FeoAB is linked with metronidazole resistance in Bacteroides fragilis ...
Bacteroides fragilis: a case study of bacteremia and septic arthritis.(CLINICAL PRACTICE) by Clinical Laboratory Science; ... CHARACTERISTICS OF Bacteroides fragilis Bacteroides fragilis is an obligate anaerobe which will appear as a gram negative ... Bacteroides fragilis can be cultured with a variety of media. B. fragilis grows towards the bottom of the thioglycollate broth ... APA style: Bacteroides fragilis: a case study of bacteremia and septic arthritis.. (n.d.) >The Free Library. (2014). Retrieved ...
Monoclonal antibodies to detect capsular diversity among Bacteroides fragilis isolates.. A Pantosti, R Colangeli, A O Tzianabos ... Monoclonal antibodies to detect capsular diversity among Bacteroides fragilis isolates.. A Pantosti, R Colangeli, A O Tzianabos ... Monoclonal antibodies to detect capsular diversity among Bacteroides fragilis isolates.. A Pantosti, R Colangeli, A O Tzianabos ... Monoclonal antibodies to detect capsular diversity among Bacteroides fragilis isolates. Message Subject (Your Name) has ...
Type strains used for in vivo competition include Bacteroides ovatus ATCC 8484, Bacteroides dorei 5_1_36/04, Bacteroides ... Bacteroides caccae CL03T12C61, B. fragilis 2_1_16, and P. copri were antagonized by the 9343 ΔT6SS mutant in a non-T6SS manner ... GA3 loci are not contained on conserved ICE and are confined to Bacteroides fragilis. Unlike GA1 and GA2 T6SS loci, most GA3 ... All four Bacteroides-type strains are sensitive to killing by the T6SS of B. fragilis 638R. ...
Meaning of bacteroides fragilis. What does bacteroides fragilis mean? Information and translations of bacteroides fragilis in ... Definition of bacteroides fragilis in the Definitions.net dictionary. ... Definitions for bacteroides fragilis. Here are all the possible meanings and translations of the word bacteroides fragilis.. ... Bacteroides fragilis. Bacteroides fragilis is a Gram-negative bacillus bacterium species, and an obligate anaerobe of the gut. ...
Bacteroides make up ~25% of the total gut microbiome. B. fragilis comprises only 2% of the total Bacteroides in the gut, yet ... Bacteroides make up ~25% of the total gut microbiome. B. fragilis comprises only 2% of the total Bacteroides in the gut, yet ... Also, blood isolates of B. fragilis did not have Type IIC CRISPR-Cas systems and had atypical Type IIIB CRISPR-Cas systems that ... Conclusions: This is the first systematic report of CRISPR-Cas systems in a wide range of B. fragilis strains from a variety of ...
Avhandling: Identification of Bacteroides fragilis from clinical samples. ... A number, of bacterial strains were examined, including B. fragilis, Bacteroides spp. other than B. fragilis and other genera. ... Identification of Bacteroides fragilis from clinical samples. Detta är en avhandling från Stockholm : Karolinska Institutet, ... Sammanfattning: The members of Bacteroides fragilis group are Gram-negative anaerobic, non-spore forming rods that are present ...
Bacteroides fragilisis Gram-negative obligatory anaerobe which usually resides in the gut of humans and animals. As an ... Bacteroides fragilis is Gram-negative obligatory anaerobe which usually resides in the gut of humans and animals. As an ... Transposon mutagenesis Bacteroides fragilis Mariner transposon Mutants This is a preview of subscription content, log in to ... Husain F, Veeranagouda Y, Hsi J et al (2013) Two multidrug-resistant clinical isolates of bacteroides fragilis carry a novel ...
Crystal structure of the putative sulfatase yidJ from Bacteroides fragilis. Northeast Structural Genomics Consortium target ... X-ray crystal structure of the putative sulfatase yidJ from Bacteroides fragilis.. Vorobiev, S.M., Abashidze, M., Seetharaman, ... Crystal structure of the putative sulfatase yidJ from Bacteroides fragilis. Northeast Structural Genomics Consortium target ... Bacteroides fragilis (strain YCH46). Mutation(s): 0 Find proteins for Q64XZ4 (Bacteroides fragilis (strain YCH46)) ...
Occurrence of Bacteroides fragilis enterotoxin gene-carrying strains in Germany and the United States. J. Clin. Microbiol. 38: ... Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin. Proc. Natl. Acad. Sci. USA 95:14979-14984. ... Bacteroides fragilis: a possible cause of acute diarrheal disease in newborn lambs. Infect. Immun. 44:241-244. ... Prevalence of Fragilysin Gene in Bacteroides fragilis Isolates from Blood and Other Extraintestinal Samples. Ina Foulon, Denis ...
The compound was tested for its antibacterial activity against Bacteroides fragilis (12 strains). ...
  • Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetaiotaomicron, and B. vulgatus: Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. (atcc.org)
  • If these putative CTns were to be demonstrated to be transmissible, this would suggest that the bft gene can be transferred from ETBF to nontoxigenic B. fragilis strains by a mechanism similar to that for the spread of antibiotic resistance genes. (asm.org)
  • The only recognized virulence factor of these strains is a toxin termed B. fragilis toxin, or BFT. (asm.org)
  • Many strains of Bacteroides species carry large self-transmissible elements called conjugative transposons (CTns). (asm.org)
  • Enterotoxigenic Bacteroides fragilis (ETBF) strains, which produce a 20- kDa zinc metalloprotease toxin (BFT), have been associated with diarrheal disease in animals and young children. (elsevier.com)
  • The investigation was performed with 9 strains of B. fragilis isolated from pregnant women in their 38th week of pregnancy, strain IPLE 323 obtained from France, strains BE17 and BE61 obtained from Netherlands and a strain 210 isolated from a case of appendicitis. (mysciencework.com)
  • 1280 strains of anaerobic bacteria from the B.fragilis group were studied. (buyantibiotics24h.net)
  • Among the strains studied, B.fragilis (65%), B.thetaiotaomicron (16%) and B.vulgatus (8% ) predominant other representatives of this group of anaerobic bacteria were much less common. (buyantibiotics24h.net)
  • We determined partial nucleotide sequences of leuB genes and compared them in seventeen strains of nine B. fragilis group species, and the regions that are conserved among Bacteroides strains but different from other species were used as a B. fragilis group-specific PCR primer set, BacLBF-BacLBR. (tokushima-u.ac.jp)
  • Among the species in the genus Bacteroides, thespecies called "B. fragilis group" areparticularly involved inhuman infections andaremedically important because they account for a major part of anaerobic isolates from clinical specimens. (tokushima-u.ac.jp)
  • Homologues of the B. fragilis C10 protease genes were present in a panel of clinical isolates, and in DNA extracted from normal human faecal microbiota. (beds.ac.uk)
  • Electron microscopic studies of laboratory-passaged and animal-passaged isolates of Bacteroides fragilis showed significantly more capsular material around the latter. (meta.org)
  • No significant difference in survival was found between animal-passaged and laboratory-passaged isolates of Bacteroides thetaiotaomicron. (meta.org)
  • Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli. (asm.org)
  • We have cloned the Bacteroides fragilis TAL2480 neuraminidase (NANase) structural gene, nanH, in Escherichia coli. (asm.org)
  • none of those with inactive disease had specimens positive for enterotoxigenic Bacteroides fragilis gene sequences. (nih.gov)
  • AF280705 Bacteroides fragilis isolate ATCC 25285 RecA (recA) gene, partial cds. (atcc.org)
  • U05887 Bacteroides fragilis ATCC 25285 beta-lactamase (cepA-85) gene, complete cds. (atcc.org)
  • AF280642 Bacteroides fragilis isolate ATCC 25285 glutamine synthetase (glnA) gene, partial cds. (atcc.org)
  • It has been reported that the bft gene is contained in a 6-kb pathogenicity island termed the B. fragilis pathogenicity island, or BfPAI ( 9 , 20 ). (asm.org)
  • By using deletion analysis and Tn1000 mutagenesis, the NANase structural gene and control region that functions in B. fragilis were localized to a 1.5- to 2.0-kb region of the insert. (asm.org)
  • The purpose of this study was to develop PCR primers that specifically and simultaneously amplify theβ-isopropylmalate dehydrogenase gene leuB in B. fragilis group species. (tokushima-u.ac.jp)
  • We identified enterotoxigenic Bacteroides fragilis in stool specimens of patients with inflammatory bowel disease and other gastrointestinal disorders. (nih.gov)
  • Enterotoxigenic Bacteroides fragilis (ETBF), a molecular subclass of the common human commensal, B. fragilis, has been associated with inflammatory bowel disease. (nih.gov)
  • Dynamics of the metallo-beta-lactamase from Bacteroides fragil. (mysciencework.com)
  • Dynamics of the metallo-beta-lactamase from Bacteroides fragilis in the presence and absence of a tight-binding inhibitor. (mysciencework.com)
  • The backbone and tryptophan side chain dynamics of the metallo-beta-lactamase from B. fragilis have been examined using (15)N NMR relaxation measurements. (mysciencework.com)
  • These putative CTns, designated CTn86 and CTn9343 for ETBF 86-5443-2-2 and NCTC 9343, respectively, differ from previously described Bacteroides species CTns in a number of ways. (asm.org)
  • Bacteroides species, saccharolytic Gram-negative obligate anaerobes, are frequently isolated from human infections such as peritonitis, abscesses and bacteremia. (tokushima-u.ac.jp)
  • To study the development of these immune cells in the context of gut microbes, researchers monocolonized germ-free mice with a model human commensal, Bacteroides fragilis, and demonstrated that this single species of bacteria could restore the development of PLZF+ innate and innate-like lymphocytes in the thymus of infant mice. (innovations-report.com)
  • The genetic element flanking the Bacteroides fragilis pathogenicity island (BfPAI) in enterotoxigenic B. fragilis (ETBF) strain 86-5443-2-2 and a related genetic element in NCTC 9343 were characterized. (asm.org)
  • In B. fragilis, NANase production from the two plasmids was inducible by free N-acetylneuraminic acid or sialic acid-containing substrates, just as in the parental TAL2480 strain. (asm.org)
  • C57BL/6 wild-type, C57BL/6, or Rag-1 mice were inoculated with saline, nontoxigenic B. fragilis or ETBF. (nih.gov)
  • This occurs before ETBF-induced mucosal permeability, suggesting that ETBF, likely through B. fragilis toxin and its action on the colonic epithelial cell, triggers mucosal immune cell Stat3 activation. (nih.gov)
  • Enterotoxigenic B. fragilis (ETBF) is an emerging enteric pathogen associated with diarrheal diseases in children, adults, and animals ( 4 , 7 - 9 ). (cdc.gov)
  • Two of these protease genes are unique to B. fragilis 638R and are associated with two large genomic insertions. (beds.ac.uk)
  • The June issue of Clinical Microbiology and Infectious Diseases published the results of a study on the antibiotic resistance of anaerobic bacteria of the group Bacteroides fragilis , isolated in Europe , conducted under the supervision of world-renowned Swedish microbiologist Karl-Erica Nord (C.-E. Nord). (buyantibiotics24h.net)
  • Therefore, there is an urgent need to continue multicenter studies of the sensitivity of anaerobic bacteria, in particular representatives of the B.fragilis group. (buyantibiotics24h.net)
  • In further proof-of-concept studies, they showed that a mutant B. fragilis lacking expression of Polysaccharide A (PSA) was unable to do the same, suggesting that specific microbial antigens could regulate this early life developmental process. (innovations-report.com)
  • Comparing the data with the results of a similar study carried out in 1992, the sensitivity of bacteria of the B.fragilis group to anti-anaerobic drugs in Europe decreased. (buyantibiotics24h.net)
  • A similar deficit in these cells was observed in mutant mice that lacked the expression of Toll like receptor 2, a receptor that recognizes bacteria and bacterial components, including B. fragilis PSA, to initiate host protective immune responses. (innovations-report.com)
  • Four homologues of the streptococcal virulence factor SpeB were identified in the B. fragilis genome. (beds.ac.uk)
  • NANase was produced from this plasmid both in E. coli and in B. fragilis. (asm.org)
  • Bacteroides fragilis is a member of the dominant Bacteroidetes phylum of the human intestinal microbiota, and is a significant opportunistic pathogen. (beds.ac.uk)
  • X83935 B.fragilis 16S rRNA (ATCC 25285T). (atcc.org)
  • These findings suggest that on mechanism whereby the capsule of B. fragilis contributes to virulence is by inhibiting opsonophagocytic killing. (meta.org)
  • The G+C contents of the BfPAI (35%) and the flanking DNA (47 to 50%) differ greatly from that reported for the B. fragilis chromosome (43%) ( http://www.sanger.ac.uk/Projects/B_fragilis ), suggesting that the BfPAI and its flanking region are two distinct genetic elements originating from different organisms. (asm.org)
  • The G+C content of the BfPAI (35%) and the flanking DNA (47 to 50%) differ greatly from that reported for the B. fragilis chromosome (42%), suggesting that the BfPAI and its flanking region are two distinct genetic elements originating from very different organisms. (elsevier.com)
  • Bacteroides fragilis , a gram-negative rod, constitutes 1% to 2% of the normal colonic bacterial microflora in humans ( 1 , 2 ). (cdc.gov)
  • A significant determinant for the broad substrate specificity of the metallo-beta-lactamases from Bacteroides fragilis and other similar organisms is the presence of a plastic substrate binding site that is nevertheless capable of tight substrate binding in the Michaelis complex. (mysciencework.com)
  • Bacteroides fragilis is a Gram-negative member of the normal human gut microbiota. (beds.ac.uk)
  • CHARACTERIZATION AND MOLECULAR ANALYSIS OF FRAGILYSIN: THE BACTEROIDES FRAGILIS TOXIN by Richard Joseph Obiso, Jr. Dr. Tracy D. Wilkins, chairman Department of Biochemistry and Anaerobic Microbiology (ABSTRACT) Bacteroides fragilis is a gram negative, anaerobic rod, that is a member of the normal colonic microflora of most mammals, and it is the anaerobe most commonly isolated from human soft tissue infections. (vt.edu)
  • abstract = "An indirect enzyme-linked immunosorbent assay was developed to detect specific Bacteroides fragilis antigen(s) in human urine. (elsevier.com)
  • The B. fragilis group is the most commonly isolated Bacteroidaceae in anaerobic infections, especially those that originate from the gastrointestinal flora. (wikipedia.org)
  • B. fragilis is involved in 90% of anaerobic peritoneal infections. (wikipedia.org)
  • Bacteroides infections at eMedicine Brook I (June 2010). (wikipedia.org)
  • The Role of Bacteroides fragilis in Pelvic Infections. (ebscohost.com)
  • Discusses the role of Bacteroides fragilis in the development of pelvic infections. (ebscohost.com)
  • Background: Bacteroides fragilis are among the most important anaerobic bacteria behind most of the anaerobic infections. (ebscohost.com)
  • Bacteroides fragilis group infections in HIV-infected patients. (ebscohost.com)
  • Bacteriodes fragilis, which is one particular type of the bacteria, is commonly found in abdominal wound infections and in the blood when the intestines are diseased. (ebscohost.com)
  • Metronidazole is the most commonly used antimicrobial for Bacteroides fragilis infections and is recommended for prophylaxis of colorectal surgery. (nih.gov)
  • In fact, B. fragilis is the most commonly isolated organism in anaerobic infections (1). (thefreelibrary.com)
  • 70% of Bacteroides infections. (frontiersin.org)
  • Therefore, B. fragilis is regarded as the most important anaerobic pathogen causing intra-abdominal abscesses and bloodstream infections. (avhandlingar.se)
  • B. fragilis is also infamous for causing serious infections. (springer.com)
  • The Gram-negative Bacteroides fragilis is the most frequently isolated bacterium from anaerobic infections. (uncg.edu)
  • Bacteroides fragilis is an opportunistic pathogen which can cause life threatening infections in humans and animals. (nrf.ac.za)
  • Fragilysin is a recently discovered virulence factor that could contribute to the pathogenesis of B. fragilis in both intestinal and soft tissue infections. (vt.edu)
  • Consequently B. fragilis is the clinical isolate most frequently found in systemic infections, this microorganism being then considered as an opportunistic pathogen [ 7 ]. (beds.ac.uk)
  • There are 2 classes of B. fragilis distinguished by their ability to secrete a zinc-dependent metalloprotease toxin, B. fragilis toxin (BFT). (biomedsearch.com)
  • Bacteroides fragilis is an obligatory anaerobic microorganism found in the normal intestinal flora of humans. (asm.org)
  • Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo. (asm.org)
  • Bacteroides fragilis may prevent intestinal inflammatory disease as this bacteria produces Polysaccharide A (PSA), which suppresses the inflammatory response of the immune system (by suppressing IL-17 production). (aurametrix.com)
  • Bacteroides fragilis is a ubiquitous anaerobic bacterium in the mammalian intestinal tract. (uchicago.edu)
  • Bacteroides fragilis, a component of the normal intestinal flora, is an obligate anaerobe capable of long-term survival in the presence of air. (ecu.edu)
  • â Gram-negative B. fragilis , though a commensal bacteria that normally lives in the human gastrointestinal tract this organism can become pathogenic under circumstances involving disruption of the normal intestinal mucosa such as trauma, or surgery. (eol.org)
  • Bacteroides distasonis shows the highest resistance rates among β-lactam antibiotics, whereas Bacteroides vulgatus shows the highest resistance levels among fluoroquinolones. (oup.com)
  • The collagen binding proteins isolated by both these independent methods were subjected to tandem mass spectroscopy for peptide identification and matched to a single hypothetical protein encoded by B. fragilis NCTC 9343 (BF0586), conserved in YCH46 (BF0662) and 638R (BF0633) and which is designated in this study as cbp1 (collagen binding protein). (nrf.ac.za)
  • In this study, we examined the enzymatic properties of the 60-kDa sialidase NanH1 of B. fragilis YCH46, which was prepared as a recombinant protein (rNanH1) in Escherichia coli. (kagawa-u.ac.jp)
  • Interestingly, there are also reports of certain genes for metabolic enzymes on B. fragilis that are actually stimulated by oxygen exposure. (thefreelibrary.com)
  • Also, blood isolates of B. fragilis did not have Type IIC CRISPR-Cas systems and had atypical Type IIIB CRISPR-Cas systems that were lacking adjacent cas genes. (frontiersin.org)
  • There are four apparent CRISPR-Cas systems in B. fragilis -three systems have adjacent cas genes. (frontiersin.org)
  • Veeranagouda Y, Husain F, Tenorio EL, Wexler HM (2014) Identification of genes required for the survival of B. fragilis using massive parallel sequencing of a saturated transposon mutant library. (springer.com)
  • Bacteroides clones ( n = 274) were isolated, identified, and screened for the presence of the virulence genes ( bft and mpII ) coding for metalloproteases. (asm.org)
  • B. fragilis clones carrying virulence genes coding for metalloproteases were more abundant in CD patients than in controls. (asm.org)
  • B. fragilis contains an extensive catalog of six trx genes and analyses indicated each is differentially regulated during oxidative conditions. (uncg.edu)
  • The B. fragilis nrdA and nrdB genes were overexpressed in Escherichia coli, and CDP reductase assays confirmed that they encoded an active enzyme. (ecu.edu)
  • nim genes were present in 4 (11.4%) B. fragilis isolates (n = 35). (elsevier.com)
  • The pathogenicity of B. fragilis is related to its production of a potent enterotoxin, a zinc-containing metalloprotease with a molecular weight of 20,000 (10) . (cdc.gov)
  • Clinical characteristics and evolution were comparable in patients with B. fragilis bacteremia with or without this enterotoxin. (asm.org)
  • Studies have attributed the aerotolerance and oxidative stress response of B. fragilis to enzymes which detoxify and protect the bacterium from oxygen radicals. (thefreelibrary.com)
  • Production of beta lactamase appears to be the main mechanism of antibiotic resistance in B. fragilis. (wikipedia.org)
  • The article focuses on the regional trend of antibiotic resistance of clinically significant isolates of the Bacteroides fragilis group in South-East Austria to ten anti-anaerobic antibiotics. (ebscohost.com)
  • Bacteroides references in Baron's Medical Microbiology (online at the NCBI bookshelf). (wikipedia.org)
  • Monoclonal antibodies to detect capsular diversity among Bacteroides fragilis isolates. (asm.org)
  • The human gut commensal Bacteroides fragilis produces sialidases that remove a terminal sialic acid from host-derived polysaccharides. (kagawa-u.ac.jp)
  • During hospitalisation the patient suffered from bacteraemia with a multidrug-resistant Bacteroides fragilis isolate. (sdu.dk)
  • Antibiotic susceptibility profiles of European Bacteroides fragilis with reduced carbapenem susceptibility. (ebscohost.com)
  • The article discusses the results of a study on the susceptibility profiles of European Bacteroides fragilis to antibiotic. (ebscohost.com)
  • IMSEAR at SEARO: Susceptibility to current antimicrobial agents of Bacteroides fragilis. (who.int)
  • Dhamabutra N, Sripayak B, Chongthalung A, Vichivanives P. Susceptibility to current antimicrobial agents of Bacteroides fragilis. (who.int)
  • Agar dilution technique susceptibility test of isolated Bacteroides fragilis from the normal healthy women's and infected gynecological patients' cervical areas were studied with current antimicrobial agents. (who.int)
  • Veeranagouda Y., Husain F., Wexler H.M. (2019) Transposon Mutagenesis of Bacteroides fragilis . (springer.com)
  • 2019. https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540052/2/Bacteroides_fragilis. (hopkinsguides.com)
  • Smalley, Darren and Rocha, Edson R. and Smith, C. Jeffrey, "Aerobic-Type Ribonucleotide Reductase in the Anaerobe Bacteroides fragilis," Journal of Bacteriology 184, no. 4 (February 2002), http://hdl.handle.net/10342/3425 (accessed March 19, 2019). (ecu.edu)
  • Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-β-lactamase at a level detectable by UV spectrophotometry. (elsevier.com)
  • Bacteroides fragilis is an obligate anaerobe which will appear as a gram negative bacillus on a gram stain. (thefreelibrary.com)
  • This micrograph depicts Bacteroides fragilis ss. (publicdomainfiles.com)
  • Public Domain Picture: This micrograph depicts Bacteroides fragilis ss. (publicdomainfiles.com)
  • DescriptionAPI ID#: 3084 English: This micrograph depicts Bacteroides fragilis ss. (eol.org)
  • Consecutive non-duplicate B. fragilis isolates from blood stream and non-blood stream specimens were included in the analysis from 2015 to 2017 period. (elsevier.com)
  • D) Agar spot assay showing sensitivity of B. fragilis strain 0878320-1 to BfUbb versus purified bovine ubiquitin. (asm.org)
  • B) Agar spot assay of BfUbb peptide overlaid with B. fragilis CM11 containing an empty vector or the vector expressing higBA . (asm.org)
  • in the en:gastrointestinal tractâ cultured in blood agar medium for 48 hours. (eol.org)
  • In addition, the majority of B. fragilis isolates are resistant to ß-lactam antimicrobial agents as well as other antibiotics. (avhandlingar.se)
  • α-Galactosidase 110A from Bacteroides fragilis, Recombinant(EC 3.2.1. (creative-enzymes.com)
  • However, the biochemical properties of recombinant B. fragilis sialidase expressed in a heterologous host remain uncharacterized. (kagawa-u.ac.jp)
  • The results and methods described in this study will be useful for preparing and characterizing recombinant proteins for other B. fragilis sialidase isoenzymes. (kagawa-u.ac.jp)
  • 2017. Gut symbiont Bacteroides fragilis secretes a eukaryotic-like ubiquitin protein that mediates intraspecies antagonism. (asm.org)
  • B. fragilis bacteriophages are commonly used as tracers of human faecal material. (wikipedia.org)
  • We assessed the occurrence and specificity of bacteriophages of Bacteroides fragilis in swine farms for their potential application in microbial source tracking. (iwaponline.com)
  • Thus, the newly developed PCR assay demonstrated potential for the environmental monitoring of Bacteroides bacteriophages, decreasing the analysis time to a few hours. (tci-thaijo.org)
  • Immunization of C57BL/10J mice with the CP over 5 wk prevents abscess formation when the mice are challenged with B. fragilis intraperitoneally. (rupress.org)
  • Bacteroides fragilis can be classified into division I (cfiA negative) and division II (cfiA positive) isolates. (elsevier.com)
  • rNanH1 preferentially hydrolyzed the sialyl α2,8 linkage and cleaved sialic acids from mucin and serum proteins (e.g., fetuin and transferrin) but not from α1-acid glycoprotein, which is similar to the previously observed biochemical properties for a native sialidase purified from B. fragilis SBT3182. (kagawa-u.ac.jp)
  • Antimicrobial therapy with ampicillin-sulbactam and anticoagulant therapy were started and the blood culture grew Bacteroides fragilis and Streptococcus intermedius . (bmj.com)
  • Molecular biology tools such as transposon mutagenesis help to decipher and understand commensal and pathogenic faces of B. fragilis . (springer.com)
  • How are glutamate dehydrogenase and glutamine synthetase activities in B. fragilis regulated under the various nitrogen conditions found within the gut and during pathogenic tissue invasion, and what role does the protein structure of these enzymes play in this regulation? (salamanderthemes.net)
  • Trauma, necrosis and ischemia → decreased redox potential → multiplication of B. fragilis and possibly → focal multiplication of facultative anaerobes. (vetstream.com)