Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.F Factor: A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.Pili, Sex: Filamentous or elongated proteinaceous structures which extend from the cell surface in gram-negative bacteria that contain certain types of conjugative plasmid. These pili are the organs associated with genetic transfer and have essential roles in conjugation. Normally, only one or a few pili occur on a given donor cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p675) This preferred use of "pili" refers to the sexual appendage, to be distinguished from bacterial fimbriae (FIMBRIAE, BACTERIAL), also known as common pili, which are usually concerned with adhesion.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Bacterial Proteins: Proteins found in any species of bacterium.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Agrobacterium tumefaciens: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Bacterial: The functional hereditary units of BACTERIA.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Fimbriae Proteins: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Bacteria, AnaerobicSex Factors: Maleness or femaleness as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or effect of a circumstance. It is used with human or animal concepts but should be differentiated from SEX CHARACTERISTICS, anatomical or physiological manifestations of sex, and from SEX DISTRIBUTION, the number of males and females in given circumstances.Sex Characteristics: Those characteristics that distinguish one SEX from the other. The primary sex characteristics are the OVARIES and TESTES and their related hormones. Secondary sex characteristics are those which are masculine or feminine but not directly related to reproduction.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Sex: The totality of characteristics of reproductive structure, functions, PHENOTYPE, and GENOTYPE, differentiating the MALE from the FEMALE organism.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Bacteria, AerobicUbiquitins: A family of proteins that are structurally-related to Ubiquitin. Ubiquitins and ubiquitin-like proteins participate in diverse cellular functions, such as protein degradation and HEAT-SHOCK RESPONSE, by conjugation to other proteins.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.SUMO-1 Protein: A 1.5-kDa small ubiquitin-related modifier protein that can covalently bind via an isopeptide link to a number of cellular proteins. It may play a role in intracellular protein transport and a number of other cellular processes.Bacterial Physiological Phenomena: Physiological processes and properties of BACTERIA.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Small Ubiquitin-Related Modifier Proteins: A class of structurally related proteins of 12-20 kDa in size. They covalently modify specific proteins in a manner analogous to UBIQUITIN.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Gram-Negative Anaerobic Bacteria: A large group of anaerobic bacteria which show up as pink (negative) when treated by the Gram-staining method.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Sex Chromosomes: The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Ubiquitin-Activating Enzymes: A class of enzymes that catalyzes the ATP-dependent formation of a thioester bond between itself and UBIQUITIN. It then transfers the activated ubiquitin to one of the UBIQUITIN-PROTEIN LIGASES.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Gene Transfer, Horizontal: The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).Sulfur-Reducing Bacteria: A group of gram-negative, anaerobic bacteria that is able to oxidize acetate completely to carbon dioxide using elemental sulfur as the electron acceptor.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Sex Determination Processes: The mechanisms by which the SEX of an individual's GONADS are fixed.Hemagglutination: The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).Unsafe Sex: Sexual behaviors which are high-risk for contracting SEXUALLY TRANSMITTED DISEASES or for producing PREGNANCY.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Enterococcus faecalis: A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.R Factors: A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.Sex Workers: People who engage in occupational sexual behavior in exchange for economic rewards or other extrinsic considerations.Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Bacteriological Techniques: Techniques used in studying bacteria.Sumoylation: A type of POST-TRANSLATIONAL PROTEIN MODIFICATION by SMALL UBIQUITIN-RELATED MODIFIER PROTEINS (also known as SUMO proteins).Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Ligases: A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Salmonella typhimurium: A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.Sex Differentiation: The process in developing sex- or gender-specific tissue, organ, or function after SEX DETERMINATION PROCESSES have set the sex of the GONADS. Major areas of sex differentiation occur in the reproductive tract (GENITALIA) and the brain.Safe Sex: Sexual behavior that prevents or reduces the spread of SEXUALLY TRANSMITTED DISEASES or PREGNANCY.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Kinetics: The rate dynamics in chemical or physical systems.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Sex Attractants: Pheromones that elicit sexual attraction or mating behavior usually in members of the opposite sex in the same species.Gammaproteobacteria: A group of the proteobacteria comprised of facultatively anaerobic and fermentative gram-negative bacteria.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Microscopy, Electron, Transmission: Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Neisseria meningitidis: A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Bacterial Infections: Infections by bacteria, general or unspecified.Flagella: A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Ubiquitin: A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Sex Determination Analysis: Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.RNA Phages: Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Intestines: The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.Proteobacteria: A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Aminoacyltransferases: Enzymes that catalyze the transfer of an aminoacyl group from donor to acceptor resulting in the formation of an ester or amide linkage. EC 2.3.2.Sex Distribution: The number of males and females in a given population. The distribution may refer to how many men or women or what proportion of either in the group. The population is usually patients with a specific disease but the concept is not restricted to humans and is not restricted to medicine.Aerobiosis: Life or metabolic reactions occurring in an environment containing oxygen.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Microbial Viability: Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.Time Factors: Elements of limited time intervals, contributing to particular results or situations.
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ProkaryotesFimbriaeAppendagesGramRecipientAntibioticsEukaryotesGenetic recombinationPathogensBacillusProteinsSpecies of bacteriaProteinBacteriophageFlagellaProduce a sex pilusSingularFungiVirusesOccursGeneCytoplasmAppendageConjugativeChromosomesReplicationResistanceProkaryoticDescribed in 1946NucleoidPhageVibrioCapable of conjugationRibosomesMembraneFertility factor or sex factorCircularCell wallsPathogenic bacteriaColi bacteriaSingle bacteriumTransposonsPlasmids occurring iCyanobacteriaOccurSecretion systemMultidrug-resistant bacteria
Prokaryotes20
- Although there are many kinds of operons in prokaryotes, the lac operon is the best understood and most widely used to teach bacterial gene regulation. (wikibooks.org)
- In the case of some prokaryotes, such as E. coli, there exists a mechanism for genetic recombination known as the sex pillus, which provides a solution for this dilemma. (wikibooks.org)
- bacterium ) are a group of microscopic, single-celled prokaryotes -that is, organisms characterized by a lack of a nucleus or any other membrane-bound organelles. (newworldencyclopedia.org)
- The term "bacteria" has been variously applied to all prokaryotes, or to a major group of them exclusive of the anaerobic archaebacteria . (newworldencyclopedia.org)
- As prokaryotes , all bacteria have a relatively simple cell structure lacking either a cell nucleus or membrane-bound organelles such as mitochondria and chloroplasts . (newworldencyclopedia.org)
- Many people know them better as 'bacteria' but, although all bacteria are prokaryotes, not all prokaryotes are bacteria. (owlcation.com)
- Some bacteria convert nitrogen gas into complex compounds that can be used by other organisms and …Bacteria help ruminant animals, such as cattle, digest organic materials.Bacteria lack a membrane-bound nucleus and other internal structures and are therefore ranked among the unicellular life-forms called prokaryotes. (academicroom.com)
- Conjugation occurs when bacteria is passed through two joined prokaryotes. (uwlax.edu)
- The discovery of the process of conjugation in prokaryotes was due to one of the most fortuitous experimental designs in recent scientific history. (springer.com)
- Once regarded as plants constituting the class Schizomycetes ("fission fungi"), bacteria are now classified as prokaryotes . (wikipedia.org)
- Although the term bacteria traditionally included all prokaryotes, the scientific classification changed after the discovery in the 1990s that prokaryotes consist of two very different groups of organisms that evolved from an ancient common ancestor . (wikipedia.org)
- 1. BACTERIA are microscopic unicellular Prokaryotes. (leavingbio.net)
- Bacteria, since they are prokaryotes, lack a nuclear membrane and membrane-bound organelles. (estrellamountain.edu)
- 4 Capsule - sticky layer of polysaccharide or protein covering the cell wall of many prokaryotes enables them to adhere to substrate or other cells (colony) prevent dehydration protects from host's immune system Fimbriae - hair-like protein appendages (aka "attachment pili") Sex pili - appendages that pull two cells together prior to DNA transfer from one cell to the other. (slideplayer.com)
- Prokaryotes include bacteria and archaea . (wikipedia.org)
- Bacteria are Prokaryotes and are Haploid (heritable unit is a single copy of the genetic material). (cram.com)
- The former life-forms - often termed prokaryotes - include bacteria and the most ancient of Earth's life-forms, the archaea. (spectrevision.net)
- Bacteria are single-celled prokaryotes, their DNA forming a long circular molecule, but not contained within a defined nucleus. (schoolbag.info)
- Bacteria are prokaryotes, which means they have no nucleus. (medicalnewstoday.com)
- Prokaryotes, like bacteria, and eukaryotes are considered to be living organisms. (scientistcindy.com)
Fimbriae16
- plural: fimbriae) can be used interchangeably, although some researchers reserve the term pilus for the appendage required for bacterial conjugation. (wikipedia.org)
- To initiate formation of a biofilm, fimbriae must attach bacteria to host surfaces for colonization during infection. (wikipedia.org)
- Mutant bacteria that lack fimbriae cannot adhere to their usual target surfaces, and thus cannot cause diseases. (wikipedia.org)
- Thus, fimbriae allow the aerobic bacteria to remain on the broth, from which they take nutrients, while they congregate near the air. (wikipedia.org)
- Many bacteria contain other extracellular structures such as flagella , fimbriae, and pili, which are used respectively for motility (movement), attachment, and conjugation (transmission of DNA between bacterial cells by contact other than fusion). (newworldencyclopedia.org)
- Pili and fimbriae are cell surface appendages present in bacteria other than flagella. (majordifferences.com)
- Pili and fimbriae are surface appendages for attachment. (majordifferences.com)
- Fimbriae (or pili ) tend to be approx. (ivyroses.com)
- Fimbriae (or pili ) help bacterial cells adhere to each other, to animal cells and to inanimate objects. (ivyroses.com)
- A single bacterium can have as many as 1,000 fimbriae. (ivyroses.com)
- Fimbriae are shorter than pili and more in no. on surface of bacteria. (biotrick.com)
- Found mainly in Gram negative organisms, Fimbriae or pili ( singlular: pilus ) are hair like filaments (tiny hollow projections) that extend from the cell membrane into the external environment. (microbeonline.com)
- Bacteria use adherence fimbriae (pili) to overcome the body's defense mechanism and cause disease. (microbeonline.com)
- Fimbriae (pili) are shorter, straighter and more numerous than bacterial flagella and are composed by subunits of protein called pilin. (microbeonline.com)
- Common pili (short attachment pili also called fimbriae). (microbeonline.com)
- Bacteria may possess no pili, one pilus, a few pili, or they may be clothed in hundreds of pili, giving them a hairy appearance (in which case the pili are sometimes called fimbriae, singular fimbria, meaning 'fringe' due to their appearance under the microscope). (cronodon.com)
Appendages6
- Some bacteria have complex life cycles involving the production of stalks and appendages (e.g. (bionity.com)
- What are the appendages of bacteria? (freezingblue.com)
- This post was most recently updated on August 26th, 2019Bacterial flagella are long, thin (about 20 nm), whip like appendages that move the bacteria towards nutrients and other attractants. (microbeonline.com)
- Pilus' is Latin for 'hair' (plural pili) and describes not so much hair-like bacterial appendages, but thin rod-like appendages that some bacteria have. (cronodon.com)
- Often adhesion occurs by means of appendages which protrude from the cell surface, such as the flagella, or by pili. (cronodon.com)
- Pili Small, stiff filamentous appendages in gram- forexrtaderguide bacteria that function in DNA exchange during bacterial conjugation. (lolimoli.ru)
Gram45
- There are two main types of bacterial cell walls, Gram positive and Gram negative, which are differentiated by their Gram staining characteristics. (bionity.com)
- Bacteria within the Deinococcus-Thermus group may also exhibit Gram positive staining behaviour but contain some cell wall structures typical of Gram negative organisms. (bionity.com)
- Bacteria can be divided into two groups (gram-positive and gram-negative) based on differences in cell wall structure as revealed by Gram staining. (newworldencyclopedia.org)
- Gram-negative bacteria have an outer, lipopolysaccharide-containing membrane and stain pink. (newworldencyclopedia.org)
- Gram-positive bacteria are more susceptible to antibiotics, while gram-negative bacteria are difficult to treat with antibiotics. (newworldencyclopedia.org)
- Conjugation and Resistance Transfer in Streptococci and Other Gram Positive Species: Plasmids, Sex Pheromones and Conjugative Transposons (A Review). (fishpond.com.au)
- Technically, the "coliform group" is defined to be all the aerobic and facultative anaerobic, non-spore-forming, Gram-negative , rod-shaped bacteria that ferment lactose with the production of gas within 48 hours at 35°C (95°F). In the body, this gas is released as flatulence. (newworldencyclopedia.org)
- In gram-negative bacteria, donor cells produce a specific plasmid-coded pilus , called the sex pilus, which attaches the donor cell to the recipient cell. (britannica.com)
- Many conjugative plasmids can be transferred between, and reproduce in, a large number of different gram-negative bacterial species. (britannica.com)
- Conjugation also has been observed in the gram-positive genus Enterococcus , but the mechanism of cell recognition and DNA transfer is different from that which occurs in gram-negative bacteria. (britannica.com)
- Gram-negative bacteria produce a sex pilus (a hairlike appendage on the surface of the bacteria) used to make contact between cells, whereas Gram-positive bacteria come into close proximity with one another and make a bridge across cells using sticky substances on the cell surface. (dummies.com)
- A series of competence proteins is produced, which have some homology but differ in the Gram negative and the Gram positive bacteria. (medpdfarticles.com)
- Type IV secretion systems (T4SS) mediate the translocation of macromolecules across the envelope of gram-negative bacteria. (asm.org)
- Gram positive bacteria has 5 times more thick peptidoglycan layer in its cell wall than gram negative. (biotrick.com)
- Gram positive bacteria has teichoic acid molecules in its cell wall while it is absent in gram negative. (biotrick.com)
- Gram negative bacteria has periplasmic space between plasma membrane and outer membrane while it is absent in gram positive bacteria. (biotrick.com)
- It is absent in gram positive bacteria. (biotrick.com)
- It makes gram negative bacteria resistant to antibiotics like penicillin etc. (biotrick.com)
- some gram-positive bacteria under harsh conditions and environmental stress, like scarcity of nitrogen and carbon sources, replicate their genome/DNA and develop a thick cell wall around their duplicated genome with a small amount of cytoplasm. (biotrick.com)
- From conjugation to T4S systems in Gram-negative bacteria: a mechanistic biology perspective. (nih.gov)
- Type IV secretion in Gram-negative and Gram-positive bacteria. (nih.gov)
- 7. Traditionally, bacteria have been grouped based on their Structure, Physiology, Molecular Composition, and Reaction to specific types of stains, (Gram Stain), rather than on their evolutionary relationships. (leavingbio.net)
- 2. A bacterial Endospore is a dormant structure that is produced by some Gram-positive bacterial species that are exposed to harsh environmental conditions. (leavingbio.net)
- Protein complexes of the IVSP family consist of multiple subunits that span the two membranes of the Gram-negative bacterial cell envelope and export proteins or DNA-protein complexes out of the cell and into the cytoplasm of a recipient cell ( Saier, 2007 ). (tcdb.org)
- There exists a lot of literature on electroporation of Gram-positive and negative bacteria. (stackexchange.com)
- Pseudomonas aeruginosa is a Gram-negative bacterium that causes opportunistic infections in immunocompromised individuals. (rupress.org)
- Like many other pathogenic Gram-negative bacteria, P. aeruginosa utilizes a type III secretion system (TTSS) to inject effector molecules into the cytoplasmic compartment of the host cell. (rupress.org)
- Although other Gram-negative bacteria, such as Salmonella typhimurium ( 9 ) and Shigella flexneri ( 10 , 11 ), have been shown to mediate a caspase-1-dependent macrophage death that is dependent on a functional TTSS, the mechanisms by which P. aeruginosa mediates TTSS-dependent macrophage cell death is largely unknown ( 12 ). (rupress.org)
- Above: a pilus projecting from the surface of a Gram negative bacterial cell. (cronodon.com)
- The T4SS is a protein pore through the cell surface, whose magnificently beautiful, basic structure has recently been elucidated in Gram-negative bacteria, in which it connects the inner and outer membrane through the periplasm . (prolekare.cz)
- Gram-positive bacteria (e.g. (cram.com)
- Gram staining is a basic microbiologic procedure for detection and identification of bacteria (see Ch. 32 ). (schoolbag.info)
- In Gram-positive bacteria, the peptidoglycan forms a thick (20-80 nm) layer external to the cell membrane, and may contain other macromolecules. (schoolbag.info)
- Figure 2.2 Construction of the cell walls of Gram-positive and Gram-negative bacteria. (schoolbag.info)
- In Gram-negative bacteria, the outer membrane is also hydrophilic, but the lipid components of the constituent molecules give hydrophobic properties as well. (schoolbag.info)
- A gram of soil typically contains about 40 million bacterial cells. (medicalnewstoday.com)
- These are called Gram-positive bacteria. (medicalnewstoday.com)
- Beta-lactamases are found in the periplasm of gram negative bacteria. (cueflash.com)
- Bacterial Strucutre & Cell Walls: Which gram-positive organism lacks a polysaccharide capsule and has a capsule made of something else? (cueflash.com)
- Bacterial Strucutre & Cell Walls: What is the major surface antigen of gram-positive cell walls and what does it do? (cueflash.com)
- Teichoic acid - unique to gram-positive bacteria. (cueflash.com)
- Bacterial Strucutre & Cell Walls: What is specific to gram-negative bacterial cell membranes? (cueflash.com)
- T4SS are found predominantly in gram-negative bacteria. (asmscience.org)
- Exceptions are the bacterial conjugation systems of gram-positive bacteria and archaea with weak homology to the conjugation systems of gram-negative bacteria, which are regarded as the evolutionarily most ancient T4SS. (asmscience.org)
- 2. (a): Danish bacteriologists Christian Gram for the first time classified bacteria on the basis of the cell wall into two groups - gram +ve and gram -ve by staining with crystal violet and safranin. (westvirginiaangerclass.com)
Recipient30
- During conjugation, a pilus emerging from the donor bacterium ensnares the recipient bacterium, draws it in close, and eventually triggers the formation of a mating bridge, which establishes direct contact and the formation of a controlled pore that allows transfer of DNA from the donor to the recipient. (wikipedia.org)
- In doing so, the bacterium is able to find another bacterium that does not have that specific plasmid, making it the recipient. (mcgilldaily.com)
- And yet, conjugation seems to benefit only the recipient. (mcgilldaily.com)
- When a bacterium donates its genetic information to another bacterium, the recipient of this selfless act will have the same advantages as the donor. (mcgilldaily.com)
- Furthermore, this recipient bacterium will likely go on to donate the copied plasmid to even more bacteria. (mcgilldaily.com)
- Conjugation occurs when two bacteria - a donor and a recipient - sidle up to each other. (geneticliteracyproject.org)
- The donor creates a tube, called a pilus, that attaches to the recipient and pulls the two cells together. (geneticliteracyproject.org)
- To proceed, conjugation requires a donor cell that expresses the fertility, or F, factor and a recipient cell without it, an F minus cell. (jove.com)
- For example, consider a scenario where the donor strain expresses ampicillin resistance and passes this on in the conjugated DNA to the recipient bacterium, but the recipient strain also has a tetracycline resistance gene not present in the donor. (jove.com)
- In this experiment, a plasmid will be used to transfer the ampicillin resistance gene via conjugation from a donor strain to a tetracycline-resistant recipient strain. (jove.com)
- Conjugation facilitates transfer of DNA from a donor to recipient. (uwlax.edu)
- The ability of a recipient bacterium to take up free DNA and become transformed is known as competence. (biocyclopedia.com)
- Conjugation is a process during which genetic information is transferred unidirectionally from a donor bacterium to a recipient through a cytoplasmic channel between the two cells. (biocyclopedia.com)
- The required cell-to-cell contact between the donor and recipient can be achieved through sex pili, through agglutinins (substances that promote cell clumping), or via pheromones (chemicals that alter the behavior of other members of the same species). (biocyclopedia.com)
- On the role of the recipient cell during conjugation in E. coli . (springer.com)
- In conjugation, the donor bacterium transfers DNA to the recipient by mating. (nih.gov)
- the plasmid can direct the synthesis of pili, carry out rolling-circle replication, and transfer genetic material to an F-recipient cell. (istudy.pk)
- The complementation of VirB1 activity was assessed by T-pilus formation, by tumor formation on wounded plants, by IncQ plasmid transfer, and by IncQ plasmid recipient assay. (asm.org)
- F + cells are donor bacteria and F cells are recipient bacteria. (biologyboom.com)
- Now this phage enters into recipient bacteria and transfer DNA of donor bacteria into the DNA of recipient bacteria. (biologyboom.com)
- in conjugation, donor and recipient cells are referred to as male and female, respectively. (brainkart.com)
- There are one to three pili expressed on an E. coli cell that carries the F factor, and one pilus will specifically interact with several molecules on the recipient cell surface (attachment). (biologyreference.com)
- To bring the donor and recipient cell into close proximity, the F pilus retracts into the donor cell by removing pilin protein monomers from the base of the pilus to draw the bacterial cells together. (biologyreference.com)
- At the end of conjugation the mating pair is broken and both the donor and the recipient cells carry an identical episomal copy of the F factor. (biologyreference.com)
- If the F factor plasmid is transferred, the recipient becomes a 'male' bacterium. (escience.ws)
- The various Inc systems are designed to mediate plasmid transfer from the donor bacterium to a recipient bacterium. (tcdb.org)
- It is a specialized kind of pili that forms the attachment between male (donor) and the female (recipient) bacteria during conjugation and acts as a conduit for the passage of DNA. (microbeonline.com)
- This pilus will retract, pulling the two bacterial cells close together and enabling one of them, the donor, to pass DNA to the recipient. (cronodon.com)
- a process whereby free, soluble, naked DNA from one bacterium (the donor) gets into another (the recipient). (cram.com)
- The finding that relaxases are transferred to recipient cells independently of DNA transfer supports the view that the secretion machinery of bacterial conjugation systems transports the relaxase, which in turn trails the DNA and directs it into the target cell. (asmscience.org)
Antibiotics18
- An efflux pump is a mechanism used by bacteria to eject antibiotics before they can affect the cell. (learner.org)
- However, they often provide an advantage to the bacteria, helping them to overcome obstacles in the environment, such as the ability to metabolize a different source of food or to synthesize a membrane protein that allows it to resist antibiotics. (mcgilldaily.com)
- Bacteria and fungi created natural antibiotics eons before drug companies turned them into medicines. (geneticliteracyproject.org)
- While overuse of antibiotics has been fingered as the driver of resistance to these drugs, the contribution of bacterial sex plays an underappreciated role, one that could bedevil efforts to fight antimicrobial resistance. (geneticliteracyproject.org)
- One small group of strains of this bacterium, however, called methicillin-resistant Staphylococcus aureus , has become resistant to multiple antibiotics and is essentially untreatable. (lumenlearning.com)
- Antimicrobial resistance (AMR) is the ability of microorganisms (including bacteria, fungi, viruses and parasites) to resist the killing capacity of antimicrobial drugs (such as antibiotics, antifungals, antivirals, antimalarials and anthelmintics). (technologynetworks.com)
- In order to be resistant, a bacterium must be able to work around or prevent the action of antibiotics. (technologynetworks.com)
- The structure provides physical protection as antibiotics can struggle to reach the heart of a developed bacterial biofilm. (technologynetworks.com)
- After this, to confirm conjugation, the conjugation mixture will be incubated on a plate containing both antibiotics leaving only the transformed bacteria. (jove.com)
- Gonorrhea is a bacterial infection that's easily cured with antibiotics. (dinheirointernet.info)
- Some bacteria are widely used in the preparation of foods, chemicals, and antibiotics. (academicroom.com)
- In industry, bacteria are important in sewage treatment and the breakdown of oil spills , the production of cheese and yogurt through fermentation , the recovery of gold, palladium, copper and other metals in the mining sector, as well as in biotechnology , and the manufacture of antibiotics and other chemicals. (wikipedia.org)
- Since the cell wall is required for bacterial survival, but is absent in eukaryotes , several antibiotics (notably the penicillins and cephalosporins ) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall only a cell membrane. (omicsgroup.org)
- R-plasmid (Resistance plasmid)provides resistant against antibiotics , Col plasmid (provides proteins that can kill other bacteria), Degradative plasmid - provides capacity to degrade/ digest certain chemicals, e.g. (biotrick.com)
- Novel multidrug and pandrug resistant bacteria are reported on a yearly basis, while the development of novel antibiotics is lacking. (frontiersin.org)
- Resistance to antibiotics in clinical bacteria has been documented for several decades ( Davies and Davies, 2010 ). (frontiersin.org)
- While plasmid replication provides an added advantage to bacteria (resistance to certain antibiotics), it also affects cell division of bacteria due to additional replication burden. (microscopemaster.com)
- AMR occurs when bacteria, fungi, viruses, and parasites are exposed for a long time to sub-therapeutic doses of drugs such as antibiotics, antifungals, antivirals, antimalarials, or anthelmintics that modify the ecology of microorganism. (intechopen.com)
Eukaryotes7
- Unlike cells of animals and other eukaryotes , bacterial cells do not contain a nucleus and rarely harbour membrane-bound organelles . (wikipedia.org)
- it occurs in different contexts in eukaryotes and bacteria. (dummies.com)
- Aaron Richardson and Jeffrey D. Palmer state: "Horizontal gene transfer (HGT) has played a major role in bacterial evolution and is fairly common in certain unicellular eukaryotes. (cfapps.io)
- This process is relatively common in bacteria, but less so in eukaryotes. (cfapps.io)
- 1. The Bacterial Cell Membrane is composed of a Lipid Bilayer similar to Eukaryotes. (leavingbio.net)
- Under the recently devised domain system, the achaea and bacteria are placed into two separate domains, with the third one containing all the eukaryotes. (estrellamountain.edu)
- Both bacteria and eukaryotes (animals, plants fungi and algae) are able to reproduce. (scientistcindy.com)
Genetic recombination7
- Eukaryote microorganisms have a specific genetic recombination step built into the formation of the sex cells, where parts of each pair of diploid chromosomes get exchanged, thus increasing the genetic diversity of the resulting spores. (dummies.com)
- vi] The product of conjugation is just the genetic recombination product of the two strains of cells. (homeomagnet.com)
- But genetic recombination takes place in bacteria. (biologyboom.com)
- There are three type of genetic recombination in bacteria. (biologyboom.com)
- The genetic recombination in which bacteria exchange genetic material through cytoplasmic bridge is called conjugation. (biologyboom.com)
- Are there mutants of bacteria that are deficient in genetic recombination? (cram.com)
- Power Point Study Guide for Microbial Genetics, Viruses (and Friends) and Genetic Recombination on Bacteria! (scientistcindy.com)
Pathogens8
- Although humans host many beneficial bacteria, certain pathogens can penetrate host defenses and cause illness or disease. (lumenlearning.com)
- Normal flora bacteria can act as opportunistic pathogens at times of lowered immunity. (lumenlearning.com)
- Invasive Bacterial Pathogens of the Intestine: Shigella Virulence Plasmids and Potential Vaccine Approaches. (fishpond.com.au)
- Subsequently, T4SS were found to mediate the translocation of proteinaceous effector molecules from several bacterial pathogens ( 3 , 11 , 12 , 20 , 36 ). (asm.org)
- Pili are small hairs that enable some pathogens to attach and adhere easily to cell surface particularly mucous membranes. (microbeonline.com)
- Genetic tests circumvent the need to culture bacteria, providing a more rapid method of identifying pathogens. (persianlab.com)
- some bacteria lack cell walls examples bacterial pathogens Mycoplasma and Ureaplasma (can cause respiratory tract infections, genital infections). (libretexts.org)
- The explosion of multidrug and pandrug resistant strains of a diversity of important bacterial pathogens, including ESBL (extended spectrum beta-lactamase), methicillin/vancomycin resistant Staphylococcus aureus (MRSA/VRSA), and pandrug resistant Acinetobacter baumannii ( Fair and Tor, 2014 ), seen over the last decade is a further indication that our current attempts to dampen the spread of resistance is not as efficient as needed. (frontiersin.org)
Bacillus5
- To take up the DNA efficiently, bacterial cells must be in a competent state, which is defined by the capability of bacteria to bind free fragments of DNA and is formed naturally only in a limited number of bacteria, such as Haemophilus , Neisseria , Streptococcus , and Bacillus . (britannica.com)
- The endospore formation is observed in common bacterial species like Clostri Bacillus. (homeomagnet.com)
- 3. Bacteria can be one of THREE Different SHAPES: the three common types of bacterial morphology - coccus, bacillus, spirillum. (leavingbio.net)
- Endospore Endospores are dormant alternate life forms produced by the genus Bacillus, the genus Clostridium, and several other genera of bacteria including Desulfotomaculum, Sporosarcina, Sporolactobacillus, Oscillospira, and Thermoactinomyces. (studyres.com)
- Examples of rod-shaped bacteria include Bacillus anthracis (B. anthracis) , or anthrax . (medicalnewstoday.com)
Proteins9
- All pili in the latter sense are primarily composed of pilin proteins, which are oligomeric. (wikipedia.org)
- About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. (bionity.com)
- The DNA contained within these plasmids encode for the expression of proteins that are not essential to the growth of bacteria. (mcgilldaily.com)
- Being F+ is not necessarily advantageous - the extra coat proteins produced make the bacterium in question more susceptible to bacteriophage infection, which is probably why F plasmids are not ubiquitous . (everything2.com)
- These bacteria have surface proteins of the live (previously R) strain. (biologyboom.com)
- This structure, called the translocon, is made up of two bacterial proteins, PopB and PopD, inserted into the host cell membrane ( 4 , 5 ). (rupress.org)
- Novel mucosal vaccines generated by genetic conjugation of heterologous proteins to pneumolysin (PLY) from Streptococcus pneumoniae. (semanticscholar.org)
- Notice the rings that form holes in the membranes through which the pilus projects - these represent complex and not well understood export and assembly systems (proteins) that transport the pilin subunits from inside the cell to the cell surface and assemble them into the pilus, which may grow as needed. (cronodon.com)
- the bacterial nucleoid has no nuclear membrane or nucleoli the bacterial nucleoid does not divide by mitosis In general it is thought that during DNA replication, each strand of the replicating bacterial DNA attaches to proteins at what will become the cell division plane. (studyres.com)
Species of bacteria7
- citation needed] Pili are responsible for virulence in the pathogenic strains of many species of bacteria including E. coli, Vibrio cholerae, and many strains of Streptococcus among others. (wikipedia.org)
- even sexual reproduction has been exhibited in some species of bacteria. (newworldencyclopedia.org)
- ˌɛ.ʃəˈɹɪ.kjə ˈkʰoʊ.laɪ] ), is one of the well known and significant species of bacteria living as gut fauna in the lower intestines of mammals . (newworldencyclopedia.org)
- For example, the large intestine of humans houses over 700 species of bacteria . (newworldencyclopedia.org)
- However, several species of bacteria are pathogenic and cause infectious diseases , including cholera , syphilis , anthrax , leprosy , and bubonic plague . (wikipedia.org)
- 3. PILI are Short, Hairlike Protein Structures found on the Surface of some species of bacteria. (leavingbio.net)
- Plasmids are today known for their ability to transfer from one species of bacteria to another through a process known as conjugation (contact between cells that is followed by transfer of DNA content). (microscopemaster.com)
Protein13
- In the context of prokaryotic cells, a fimbria (or a pilus ) is a protein rod that is sometimes called a proteinaceous appendage . (ivyroses.com)
- A typical bacterial replicon may consist of a number of elements, such as the gene for plasmid-specific replication initiation protein (Rep), repeating units called iterons , DnaA boxes, and an adjacent AT-rich region. (wikipedia.org)
- Bacteria moves with the help of Flagel l a made up of flagellin protein. (biotrick.com)
- Protein-Injection Machines in Bacteria. (nih.gov)
- These systems are very promiscuous, being capable of transporting DNA-protein complexes into other bacteria, yeast and plants. (tcdb.org)
- Alvarez-Martinez and Christie (2009) classify T4SSs on the basis of function as conjugation machines, effector translocators, or DNA release/uptake systems, but this approach has its limitations, because conjugation systems also translocate protein substrates independently of DNA, and some effector translocator systems also conjugatively transfer DNA to target cells. (tcdb.org)
- The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the outside of the bacterial cell. (tcdb.org)
- The N-terminal lytic transglycosylase domain provides localized lysis of the peptidoglycan cell wall to allow insertion of the IVSP complex while the C-terminal VirB1* promotes T-pilus assembly through protein-protein interactions with T-pilus subunits. (tcdb.org)
- A pilus is composed of subunits of the protein pilin. (microbeonline.com)
- The general flow of information in a bacterial cell is from DNA (which contains the genetic information) to messenger RNA (mRNA) (which acts as a blueprint for protein construction) to the actual protein itself. (persianlab.com)
- Genetic conjugation of components in two pneumococcal fusion protein vaccines enhances paediatric mucosal immune responses. (semanticscholar.org)
- Notice that the pilus is depicted as being made up of many spheres, these are the subunits, each one is a molecule of protein called pilin (fimbrin). (cronodon.com)
- Lack of the leucine-responsive regulatory protein (Lrp) causes a decrease in R100 plasmid transfer, indicating that Lrp is an activator of R100-mediated conjugation. (isciii.es)
Bacteriophage2
- It is the process of transfer of'DNA fragmentfrom one bacteria into the other with the help of a bacteriophage. (homeomagnet.com)
- As you may recall, the filamentous bacteriophage only infected male bacteria - i.e. those with pili on their surfaces. (escience.ws)
Flagella8
- Movement produced by type IV pili is typically jerky, so it is called twitching motility, as opposed to other forms of bacterial motility such as that produced by flagella. (wikipedia.org)
- Bacterial type IV pili are similar in structure to the component flagellins of archaeal flagella. (wikipedia.org)
- bacteria may have one, a few, or many flagella in different positions on the cell. (powershow.com)
- So flagella provides mobility to bacteria. (biotrick.com)
- It is shorter than flagella and helps in adhesion & exchange of genetic information(sex pili) . (biotrick.com)
- Not all bacteria have flagella, the bacterium in the picture above has no flagella and is non-motile but eventually it settles and makes contact with the surface (if it had flagella it may have actively swam toward the surface) and once its pili touch the surface they stick to it, because their ends are sticky. (cronodon.com)
- and often various external structures such as a glycocalyx, flagella, and pili. (studyres.com)
- External to this wall may be capsules, flagella and pili. (schoolbag.info)
Produce a sex pilus1
- The F factor is a bacterial DNA sequence that allows a bacterium to produce a sex pilus and a conjugation bridge with another conjugant, necessary for conjugation. (newworldencyclopedia.org)
Singular2
- common noun bacteria , singular bacterium ) are a type of biological cell . (wikipedia.org)
- common noun bacteria , singular bacterium ) constitute a large domain of prokaryotic microorganisms . (alchetron.com)
Fungi1
- One example are molds and fungi that are able to produce molecules that have an antibiotic effect on nearby bacteria -- such as the betalactam molecules that we produce into penicillins. (wikibooks.org)
Viruses4
- During the process of conjugation, Bacteria and viruses have DNA too. (dinheirointernet.info)
- The term's early usage included any bacterial genetic material that exists extrachromosomally for at least part of its replication cycle, but because that description includes bacterial viruses, the notion of plasmid was refined over time to comprise genetic elements that reproduce autonomously. (wikipedia.org)
- Genetic information in bacteria and many viruses is encoded in DNA, but some viruses use RNA. (nih.gov)
- Bacteria burst and release new phage viruses. (biologyboom.com)
Occurs3
- Bacterial conjugation occurs when a donor cell bacterium that has a certain plasmid extends a long "arm" called a pilus. (mcgilldaily.com)
- Both asexual and sexual reproduction occurs in bacteria. (biologyboom.com)
- 5. (a): In sexual reproduction, syngamy and meiotic division takes place but in bacteria, during sexual reproduction there is no formation of gametes hence no syngamy and reduction division occurs, bacteria lack alternation of generation. (westvirginiaangerclass.com)
Gene7
- Still others are trying to exploit natural mechanisms , such as restriction-modifying enzymes or CRISPR-Cas gene-editing systems, that bacteria use to defend against invading genomes. (geneticliteracyproject.org)
- This gene expression can be used to confirm successful conjugation. (jove.com)
- In this procedure, the ampicillin resistance gene will be transferred from the WM3064 strain of E. coli to the J53 strain of E. coli via conjugation. (jove.com)
- killing gene into the bacteria that. (dinheirointernet.info)
- However, gene sequences can be used to reconstruct the bacterial phylogeny , and these studies indicate that bacteria diverged first from the archaeal/eukaryotic lineage. (worldheritage.org)
- The first observation of a bacterial gene called MCR-1 in the United States has scientists worried, if not surprised. (scienceblogs.com)
- The gene provides resistance to colistin, an antibiotic with nasty side effects used to combat multidrug-resistant bacteria. (scienceblogs.com)
Cytoplasm6
- Inclusion bodies: T hese are organic or inorganic granules present in the cytoplasm of bacteria. (biotrick.com)
- 1. Bacteria are Typically composed of a Cell Wall, a Cell Membrane, and Cytoplasm. (leavingbio.net)
- The CYTOPLASM of Bacterial cells is made of a Viscous (Thick Liquid) solution of Ribosomes and DNA. (leavingbio.net)
- Some bacteria also have PLASMIDS, self-replicating loops of DNA, in their Cytoplasm. (leavingbio.net)
- Cytoplasm In bacteria, the cytoplasm refers to everything enclosed by the cytoplasmic membrane. (studyres.com)
- About 80% of the cytoplasm of bacteria is composed of water. (studyres.com)
Appendage2
- plural : pili) is a hair-like appendage found on the surface of many bacteria. (wikipedia.org)
- A pilus is a hair-like appendage many of which are present on the surface of many bacteria. (ivyroses.com)
Conjugative2
- Not all bacteria can make conjugative pili, but conjugation can occur between bacteria of different species. (wikipedia.org)
- It plays a major role in conjugation in E. coli , and it was the first conjugative plasmid to be described. (istudy.pk)
Chromosomes3
- In addition to regular chromosomes, some bacteria possess small rings of independently replicating DNA molecules called as plasmid. (biotrick.com)
- We call such engineered F' plasmids ' BACs ' or Bacterial Artificial Chromosomes. (escience.ws)
- And bacterial chromosomes can contain still other transmissible elements, including some that can be mobilized by ICEs, such as integrative and mobilizable elements (IMEs) . (prolekare.cz)
Replication2
- Organise the following events in the order in which they occur in bacterial DNA replication. (garlandscience.com)
- During normal growth, homologous recombination is used by the bacteria to restart stalled DNA replication forks, which can result from replication error. (cram.com)
Resistance5
- To fight antimicrobial resistance, researchers are exploring strategies to inhibit bacterial conjugation. (geneticliteracyproject.org)
- This is compounded by the fact that the molecular tools available for the study of resistance mechanisms are more well-established in bacteria than in other microbes, and the sheer number of antibacterial classes offers greater scope for studying modes of action and resistance. (technologynetworks.com)
- Altering bacterial mode of growth can also confer resistance to antibiotic treatment. (technologynetworks.com)
- Plasmids, rather than containing all the information for everyday life, were essentially a power-boost, giving bacteria the ability to be better versions of themselves-making their own nutrients or acquiring resistance to a specific antibiotic for instance. (nih.gov)
- The drug abuse known to occur during growth of animals intended for food production, because of their use as either a prophylactic or therapeutic treatment, promotes the emergence of bacterial drug resistance. (intechopen.com)
Prokaryotic2
- In many cases the flagellum of a prokaryotic cell (e.g. a bacterium) is responsible for the motility , i.e. ability to move spontaneously and actively - consuming energy in the process , of the cell. (ivyroses.com)
- The outer surface of a prokaryotic cell presents a set of features to the external environment that includes structural materials (glycoproteins and lipopolysaccharides), transport machinery (amino acid or sugar transport complexes), and cell-to-cell interaction apparatus the F or sex pilus. (americorpshealth.biz)
Described in 19461
- Conjugation was first described in 1946 by Lederberg and Tatum, based on studies showing that the intestinal bacteria E.coli uses a process resembling sex to exchange circular, extrachromosomal elements, now known as plasmids. (msu.edu)
Nucleoid4
- It is a comparatively rare process of reproduction of bacteria observed in few like Rhodopseudomonas.It is a process by which the vegetative cell forms a lateral protuburance in the form of an outer bulge containing a fragment of genetic material or nucleoid in it. (homeomagnet.com)
- Bacterial DNA is circular and arrayed in a region of the cell known as the nucleoid , shown in Figure 3. (estrellamountain.edu)
- The nucleoid is the genetic material of the bacterium. (studyres.com)
- 7. (c): Bacteria has no nuclear membrane hence it is called as nucleoid. (westvirginiaangerclass.com)
Phage2
- The ligation mix is then packaged into phage particles and the DNA is transfected into the bacterial host. (wikipedia.org)
- Bacterial endonucleases enzymes destroy the phage virus. (biologyboom.com)
Vibrio1
- A few scientists noticed in the late 1960s that the marine bacteria Vibrio fischeri appeared to coordinate among themselves the production of chemicals that produced bioluminescence, waiting until a certain number of them were in the neighborhood before firing up their light-making machinery. (spectrevision.net)
Capable of conjugation1
- The F plasmid is the best characterised example of this, but other plasmids are also capable of conjugation. (everything2.com)
Ribosomes1
- A typical bacterium may have as many as 15,000 ribosomes. (studyres.com)
Membrane6
- At low surface area-to-volume ratios the diffusion of nutrients and waste products across the bacterial cell membrane limits the rate at which microbial metabolism can occur, making the cell less evolutionarily fit. (bionity.com)
- Most bacteria do not have a membrane. (dinheirointernet.info)
- They have no membrane-bound organelles like mitochondria, chloroplast,nucleus, golgi appratus, endoplasmic reticulum.All these are absent in bacteria. (biotrick.com)
- Mesosomes are folded invaginations in the plasma membrane of bacteria. (biotrick.com)
- 2. In Bacteria, the cell membrane contains Enzymes that Perform the Reactions of Cellular Respiration. (leavingbio.net)
- All bacteria also have a cell membrane, as well as a cell wall, as shown in Figure 4. (estrellamountain.edu)
Fertility factor or sex factor1
- The F factor is also calk fertility factor or sex factor and is represented by an extra nuclear circular DNA present only in male. (homeomagnet.com)
Circular2
- Plasmid Plasmid- Small molecules of autonomously replicating, circular, extrachromosomal DNA found in many bacteria. (studyres.com)
- The genetic information of bacteria is carried in a long, double-stranded (ds), circular molecule of DNA ( Fig. 2.1 ). (schoolbag.info)
Cell walls3
- What chemicals or materials are found in bacteria cell walls? (freezingblue.com)
- Bacterial Strucutre & Cell Walls: Where is beta-lactamase in bacteria? (cueflash.com)
- Bacterial Strucutre & Cell Walls: What are spores made of and what do they do? (cueflash.com)
Pathogenic bacteria2
- This post was most recently updated on August 26th, 2019Morphological characteristics of pathogenic bacteria helps us to identify them, when we stain microbiological specimens and view them under microscope. (microbeonline.com)
- The World Health Organization considers the emergence of multidrug-resistant pathogenic bacteria as a health problem, since the illnesses caused by them complicate the treatment and increase the morbidity and mortality rates. (intechopen.com)
Coli bacteria7
- Under certain conditions E. coli bacteria may become harmful & cause infection in the intestinal or urinogenital tracts. (sciencephoto.com)
- Low-temperature electron micrograph of a cluster of E. coli bacteria, magnified 10,000 times. (newworldencyclopedia.org)
- The number of individual E. coli bacteria in the feces that a human excretes in one day averages between 100 billion and 10 trillion. (newworldencyclopedia.org)
- All the different kinds of fecal coli bacteria (i.e. (newworldencyclopedia.org)
- The novel germ has some virulence abilities of a class of pathogenic E. coli bacteria called entero-aggregative E. coli (#EAEC). (biofortified.org)
- DNA of phages virus enter into E.coli bacteria. (biologyboom.com)
- 6. (a): E. coli bacteria acts as a human symbionts and it is found in human intestine, synthesize vitamin K and B and also help in food fermentation. (westvirginiaangerclass.com)
Single bacterium2
- The endospore germinates in favourable condition and develops new single bacterium. (biologyboom.com)
- Bacteria shaped like a ball are called cocci , and a single bacterium is a coccus . (medicalnewstoday.com)
Transposons1
- Trimethoprim-Resistant Bacteria in Hospital and in the Community: Spread of Plasmids and Transposons. (fishpond.com.au)
Plasmids occurring i1
- Some important groups of plasmids occurring in bacteria are given below. (newworldencyclopedia.org)
Cyanobacteria6
- The largely photosynthetic blue-green algae, or cyanobacteria , were once classified as algae , but are now considered bacteria. (newworldencyclopedia.org)
- The old taxonomic Kingdom Monera consisted of the bacteria (meaning the true bacteria and cyanobacteria, or photosynthetic bacteria) as well as the archea. (estrellamountain.edu)
- Bacteria (technically the Eubacteria ) and blue-green bacteria (the blue-green algae when I was a student back in the 1970s), or cyanobacteria are the major forms of life in this domain, examples of which are shown in Figure 2. (estrellamountain.edu)
- Two cyanobacteria, Oscillatoria (left) and Nostoc (right), and a collection of heterotrophic bacteria (center bottom). (estrellamountain.edu)
- The photosynthetic cyanobacteria are arguably the most successful bacteria, and changed the early atmosphere of the earth by oxygenating it. (wikipedia.org)
- Monera includes bacteria, mycoplasmas, cyanobacteria (blue green alga) and actinomycetes. (westvirginiaangerclass.com)
Occur5
- Despite the fact that bacteria reproduce exclusively asexually, the sharing of genetic information within and between related species is now recognized to be quite common and to occur in at least three fundamentally different ways. (brainkart.com)
- Bacteria occur in almost every environment on Earth, from the bottom of the ocean floor, deep inside solid rock, to the cooling jackets of nuclear reactors. (estrellamountain.edu)
- Conjugation between bacterial genera and species and also interkingdom conjugation (bacteria to yeast and bacteria to plants [ 6 )]) have been shown to occur. (asm.org)
- Symbiotic nitrogen fixing bacteria of the genus Rhizobium occur in the root nodules of a number of legumes. (westvirginiaangerclass.com)
- Root nodules containing symbiotic nitrogen bacteria also occur in Casuarina and Alnus. (westvirginiaangerclass.com)