Bacteriorhodopsins: Rhodopsins found in the PURPLE MEMBRANE of halophilic archaea such as HALOBACTERIUM HALOBIUM. Bacteriorhodopsins function as an energy transducers, converting light energy into electrochemical energy via PROTON PUMPS.Optics and Photonics: A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range.Optical Devices: Products or parts of products used to detect, manipulate, or analyze light, such as LENSES, refractors, mirrors, filters, prisms, and OPTICAL FIBERS.Halobacterium salinarum: A species of halophilic archaea found in salt lakes. Some strains form a PURPLE MEMBRANE under anaerobic conditions.Halobacterium: A genus of HALOBACTERIACEAE whose growth requires a high concentration of salt. Binary fission is by constriction.Refractometry: Measurement of the index of refraction (the ratio of the velocity of light or other radiation in the first of two media to its velocity in the second as it passes from one into the other).Purple Membrane: Functionally and structurally differentiated, purple-pigmented regions of the cytoplasmic membrane of some strains of Halobacterium halobium. The membrane develops under anaerobic conditions and is made almost entirely of the purple pigment BACTERIORHODOPSINS. (From Singleton & Sainsbury Dictionary of Microbiology and Molecular Biology, 2d ed)Schiff Bases: Condensation products of aromatic amines and aldehydes forming azomethines substituted on the N atom, containing the general formula R-N:CHR. (From Grant & Hackh's Chemical Dictionary, 5th ed)Retinaldehyde: A carotenoid constituent of visual pigments. It is the oxidized form of retinol which functions as the active component of the visual cycle. It is bound to the protein opsin forming the complex rhodopsin. When stimulated by visible light, the retinal component of the rhodopsin complex undergoes isomerization at the 11-position of the double bond to the cis-form; this is reversed in "dark" reactions to return to the native trans-configuration.PhotochemistryElectronics, Medical: The research and development of ELECTRICAL EQUIPMENT AND SUPPLIES for such medical applications as diagnosis, therapy, research, anesthesia control, cardiac control, and surgery. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Memory: Complex mental function having four distinct phases: (1) memorizing or learning, (2) retention, (3) recall, and (4) recognition. Clinically, it is usually subdivided into immediate, recent, and remote memory.Transistors, Electronic: Electrical devices that are composed of semiconductor material, with at least three connections to an external electronic circuit. They are used to amplify electrical signals, detect signals, or as switches.Directed Molecular Evolution: The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.Artificial Intelligence: Theory and development of COMPUTER SYSTEMS which perform tasks that normally require human intelligence. Such tasks may include speech recognition, LEARNING; VISUAL PERCEPTION; MATHEMATICAL COMPUTING; reasoning, PROBLEM SOLVING, DECISION-MAKING, and translation of language.Portraits as Topic: Graphic representations, especially of the face, of real persons, usually posed, living or dead. (From Thesaurus for Graphic Materials II, p540, 1995)Myosins: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.Protons: Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.Proton Pumps: Integral membrane proteins that transport protons across a membrane. This transport can be linked to the hydrolysis of ADENOSINE TRIPHOSPHATE. What is referred to as proton pump inhibitors frequently is about POTASSIUM HYDROGEN ATPASE.Bungarus: A genus of poisonous snakes of the subfamily Elapinae of the family ELAPIDAE. They comprise the kraits. Twelve species are recognized and all inhabit southeast Asia. They are considered extremely dangerous. (Moore: Poisonous Snakes of the World, 1980, p120)Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.DimethylaminesBacterial Proton-Translocating ATPases: Membrane-bound proton-translocating ATPases that serve two important physiological functions in bacteria. One function is to generate ADENOSINE TRIPHOSPHATE by utilizing the energy provided by an electrochemical gradient of protons across the cellular membrane. A second function is to counteract a loss of the transmembrane ion gradient by pumping protons at the expense of adenosine triphosphate hydrolysis.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Mitochondrial Proton-Translocating ATPases: Proton-translocating ATPases responsible for ADENOSINE TRIPHOSPHATE synthesis in the MITOCHONDRIA. They derive energy from the respiratory chain-driven reactions that develop high concentrations of protons within the intermembranous space of the mitochondria.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Computer Security: Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.Confidentiality: The privacy of information and its protection against unauthorized disclosure.Ascomycota: A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Privacy: The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)Rhodopsin: A purplish-red, light-sensitive pigment found in RETINAL ROD CELLS of most vertebrates. It is a complex consisting of a molecule of ROD OPSIN and a molecule of 11-cis retinal (RETINALDEHYDE). Rhodopsin exhibits peak absorption wavelength at about 500 nm.Brassica napus: A plant species of the family BRASSICACEAE best known for the edible roots.Neodymium: Neodymium. An element of the rare earth family of metals. It has the atomic symbol Nd, atomic number 60, and atomic weight 144.24, and is used in industrial applications.Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.Textbooks as Topic: Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.Thioamides: Organic compounds containing the radical -CSNH2.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.Physics: The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)BooksHaemosporida: An order of heteroxenous protozoa in which the macrogamete and microgamont develop independently. A conoid is usually absent.Plasmodium: A genus of protozoa that comprise the malaria parasites of mammals. Four species infect humans (although occasional infections with primate malarias may occur). These are PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM OVALE, and PLASMODIUM VIVAX. Species causing infection in vertebrates other than man include: PLASMODIUM BERGHEI; PLASMODIUM CHABAUDI; P. vinckei, and PLASMODIUM YOELII in rodents; P. brasilianum, PLASMODIUM CYNOMOLGI; and PLASMODIUM KNOWLESI in monkeys; and PLASMODIUM GALLINACEUM in chickens.Plasmodium falciparum: A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.Malaria: A protozoan disease caused in humans by four species of the PLASMODIUM genus: PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM OVALE; and PLASMODIUM MALARIAE; and transmitted by the bite of an infected female mosquito of the genus ANOPHELES. Malaria is endemic in parts of Asia, Africa, Central and South America, Oceania, and certain Caribbean islands. It is characterized by extreme exhaustion associated with paroxysms of high FEVER; SWEATING; shaking CHILLS; and ANEMIA. Malaria in ANIMALS is caused by other species of plasmodia.Parasites: Invertebrate organisms that live on or in another organism (the host), and benefit at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.Malaria, Falciparum: Malaria caused by PLASMODIUM FALCIPARUM. This is the severest form of malaria and is associated with the highest levels of parasites in the blood. This disease is characterized by irregularly recurring febrile paroxysms that in extreme cases occur with acute cerebral, renal, or gastrointestinal manifestations.Plasmodium vivax: A protozoan parasite that causes vivax malaria (MALARIA, VIVAX). This species is found almost everywhere malaria is endemic and is the only one that has a range extending into the temperate regions.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Halobacteriales: An order of extremely halophilic archaea, in the kingdom EURYARCHAEOTA. They occur ubiquitously in nature where the salt concentration is high, and are chemoorganotrophic, using amino acids or carbohydrates as a carbon source.Brassicaceae: A plant family of the order Capparales, subclass Dilleniidae, class Magnoliopsida. They are mostly herbaceous plants with peppery-flavored leaves, due to gluconapin (GLUCOSINOLATES) and its hydrolysis product butenylisotrhiocyanate. The family includes many plants of economic importance that have been extensively altered and domesticated by humans. Flowers have 4 petals. Podlike fruits contain a number of seeds. Cress is a general term used for many in the Brassicacea family. Rockcress is usually ARABIS; Bittercress is usually CARDAMINE; Yellowcress is usually RORIPPA; Pennycress is usually THLASPI; Watercress refers to NASTURTIUM; or RORIPPA or TROPAEOLUM; Gardencress refers to LEPIDIUM; Indiancress refers to TROPAEOLUM.Salt-Tolerant Plants: Plants that can grow well in soils that have a high SALINITY.Acidithiobacillus: A genus of gram-negative rod-shaped bacteria in the class GAMMAPROTEOBACTERIA. They are obligately acidophilic and aerobic, using reduced SULFUR COMPOUNDS to support AUTOTROPHIC GROWTH.Wyoming

Location of a cation-binding site in the loop between helices F and G of bacteriorhodopsin as studied by 13C NMR. (1/1144)

The high-affinity cation-binding sites of bacteriorhodopsin (bR) were examined by solid-state 13C NMR of samples labeled with [3-13C]Ala and [1-13C]Val. We found that the 13C NMR spectra of two kinds of blue membranes, deionized (pH 4) and acid blue at pH 1.2, were very similar and different from that of the native purple membrane. This suggested that when the surface pH is lowered, either by removal of cations or by lowering the bulk pH, substantial change is induced in the secondary structure of the protein. Partial replacement of the bound cations with Na+, Ca2+, or Mn2+ produced additional spectral changes in the 13C NMR spectra. The following conclusions were made. First, there are high-affinity cation-binding sites in both the extracellular and the cytoplasmic regions, presumably near the surface, and one of the preferred cation-binding sites is located at the loop between the helix F and G (F-G loop) near Ala196, consistent with the 3D structure of bR from x-ray diffraction and cryoelectron microscopy. Second, the bound cations undergo rather rapid exchange (with a lifetime shorter than 3 ms) among various types of cation-binding sites. As expected from the location of one of the binding sites, cation binding induced conformational alteration of the F-G interhelical loop.  (+info)

Distortion of the L-->M transition in the photocycle of the bacteriorhodopsin mutant D96N: a time-resolved step-scan FTIR investigation. (2/1144)

The D96N mutant of bacteriorhodopsin has often been taken as a model system to study the M intermediate of the wild type photocycle due to the long life time of the corresponding intermediate of the mutant. Using time-resolved step-scan FTIR spectroscopy in combination with a sample changing wheel we investigated the photocycle of the mutant with microsecond time resolution. Already after several microseconds an intermediate similar to the MN state is observed, which contrasts with the M state of the wild type protein. At reduced hydration M and N intermediates similar to those of wild type BR can be detected. These results have a bearing on the interpretation of the photocycle of this mutant. A mechanism is suggested for the fast rise of MN which provides some insight into the molecular events involved in triggering the opening of the cytosolic channel also of the wild type protein.  (+info)

Molecular modeling of mu opioid receptor and receptor-ligand interaction. (3/1144)

AIM: To construct the 3D structural model of mu opioid receptor (mu OR) and study the interaction between mu OR and fentanyl derivatives. METHODS: The 3D structure of mu OR was modeled using the bacteriorhodopsin (bRh) as a template, in which the alignments of transmembrane (TM) of bRh and mu OR were achieved by scoring the alignment between the amino acid sequence of mu OR and the structure of bRh. The fentanyl derivatives were docked into the 7 helices of mu OR and the binding energies were calculated. RESULTS: (1) The receptor-ligand interaction models were obtained for fentanyl derivatives. (2) In these models, the fundamental binding sites were possibly Asp147 and His297. The negatively charged oxygen of Asp147 and the positively charged ammonium group of ligand formed the potent electrostatic and hydrogen-binding interactions. Whereas the interactions between the positively charged nitrogen of His297 and the carbonyl oxygen of ligand were weak. In addition, there were some pi-pi interactions between the receptor and the ligand. (3) The binding energies of the receptor-ligand complexes had a good correlation with the analgesic activities (-lg ED50) of the fentanyl derivatives. CONCLUSION: This model is helpful for understanding the receptor-ligand interaction and for designing novel mu OR selective ligands.  (+info)

Molecular modeling of interaction between delta opioid receptor and 3-methylfentanylisothiocyanate. (4/1144)

AIM: To construct a 3D structural model of delta opioid receptor (delta OR) and study its interaction with 3-methylfentanylisothiocyanate (SuperFIT). METHODS: Using the bacteriohodopsin as a template, the 3D structure of delta OR was modeled; SuperFIT was docked into its inside. RESULTS: The interaction model between delta OR and (3R, 4S)-SuperFIT was achieved, in which the important binding sites possibly were Asp128, Ser106, Phe104, Tyr308, and Pro315. Asp128 formed the electrostatic and hydrogen-binding interactions with the protonated nitrogen on piperidine of the ligand. Ser106 formed the electrostatic interaction with the N atom of isothiocyano group of the ligand; whereas Phe104, Tyr308, and Pro315 formed the hydrophobic interactions with the S atom of isothiocyano group. In addition, there were some other interactions between delta OR and the ligand. CONCLUSION: The residues Phe104, Tyr308, Pro315, and Ser106 of delta OR are crucial to the delta selectivity of the ligand, which is beneficial for designing novel delta-selective ligand.  (+info)

Interpretation of the spatial charge displacements in bacteriorhodopsin in terms of structural changes during the photocycle. (5/1144)

We have recently introduced a method, made possible by an improved orienting technique using a combination of electric and magnetic fields, that allows the three-dimensional detection of the intramolecular charge displacements during the photocycle of bacteriorhodopsin. This method generates electric asymmetry, a prerequisite for the detection of electric signal on the macroscopic sample, in all three spatial dimensions. Purple membrane fragments containing bacteriorhodopsin were oriented so that their permanent electric dipole moment vectors were perpendicular to the membrane plane and pointed in the same direction. The resulting cylindrical symmetry was broken by photoselection, i. e., by flash excitation with low intensity linearly polarized light. From the measured electric signals, the three-dimensional motion of the electric charge center in the bacteriorhodopsin molecules was calculated for the first 400 microseconds. Simultaneous absorption kinetic recording provided the time-dependent concentrations of the intermediates. Combining the two sets of data, we determined the discrete dipole moments of intermediates up to M. When compared with the results of current molecular dynamics calculations, the data provided a decisive experimental test for selecting the optimal theoretical model for the proton transport and should eventually lead to a full description of the mechanism of the bacteriorhodopsin proton pump.  (+info)

Simulation analysis of the retinal conformational equilibrium in dark-adapted bacteriorhodopsin. (6/1144)

In dark-adapted bacteriorhodopsin (bR) the retinal moiety populates two conformers: all-trans and (13,15)cis. Here we examine factors influencing the thermodynamic equilibrium and conformational transition between the two forms, using molecular mechanics and dynamics calculations. Adiabatic potential energy mapping indicates that whereas the twofold intrinsic torsional potentials of the C13==C14 and C15==N16 double bonds favor a sequential torsional pathway, the protein environment favors a concerted, bicycle-pedal mechanism. Which of these two pathways will actually occur in bR depends on the as yet unknown relative weight of the intrinsic and environmental effects. The free energy difference between the conformers was computed for wild-type and modified bR, using molecular dynamics simulation. In the wild-type protein the free energy of the (13,15)cis retinal form is calculated to be 1.1 kcal/mol lower than the all-trans retinal form, a value within approximately kBT of experiment. In contrast, in isolated retinal the free energy of the all-trans state is calculated to be 2.1 kcal/mol lower than (13,15)cis. The free energy differences are similar to the adiabatic potential energy differences in the various systems examined, consistent with an essentially enthalpic origin. The stabilization of the (13,15)cis form in bR relative to the isolated retinal molecule is found to originate from improved protein-protein interactions. Removing internal water molecules near the Schiff base strongly stabilizes the (13,15)cis form, whereas a double mutation that removes negative charges in the retinal pocket (Asp85 to Ala; Asp212 to Ala) has the opposite effect.  (+info)

Chloride ion binding to bacteriorhodopsin at low pH: an infrared spectroscopic study. (7/1144)

Bacteriorhodopsin (bR) and halorhodopsin (hR) are light-induced ion pumps in the cell membrane of Halobacterium salinarium. Under normal conditions bR is an outward proton transporter, whereas hR is an inward Cl- transporter. There is strong evidence that at very low pH and in the presence of Cl-, bR transports Cl- ions into the cell, similarly to hR. The chloride pumping activity of bR is connected to the so-called acid purple state. To account for the observed effects in bR a tentative complex counterion was suggested for the protonated Schiff base of the retinal chromophore. It would consist of three charged residues: Asp-85, Asp-212, and Arg-82. This quadruplet (including the Schiff base) would also serve as a Cl- binding site at low pH. We used Fourier transform infrared difference spectroscopy to study the structural changes during the transitions between the normal, acid blue, and acid purple states. Asp-85 and Asp-212 were shown to participate in the transitions. During the normal-to-acid blue transition, Asp-85 protonates. When the pH is further lowered in the presence of Cl-, Cl- binds and Asp-212 also protonates. The binding of Cl- and the protonation of Asp-212 occur simultaneously, but take place only when Asp-85 is already protonated. It is suggested that HCl is taken up in undissociated form in exchange for a neutral water molecule.  (+info)

Time-resolved absorption and photothermal measurements with sensory rhodopsin I from Halobacterium salinarum. (8/1144)

An expansion accompanying the formation of the first intermediate in the photocycle of transducer-free sensory rhodopsin I (SRI) was determined by means of time-resolved laser-induced optoacoustic spectroscopy. For the native protein (SRI-WT), the absolute value of the expansion is approximately 5.5 mL and for the mutant SRI-D76N, approximately 1.5 mL per mol of phototransformed species (in 0.5 M NaCl), calculated by using the formation quantum yield for the first intermediate (S610) of Phi610 = 0.4 +/- 0.05 for SRI-WT and 0.5 +/- 0.05 for SRI-D76N, measured by laser-induced optoacoustic spectroscopy and by laser flash photolysis. The similarity in Phi610 and in the determined value of the energy level of S610, E610 = (142 +/- 12) kJ/mol for SRI-WT and SRI-D76N indicates that Asp76 is not directly involved in the first step of the phototransformation. The increase with pH of the magnitude of the structural volume change for the formation of S610 in SRI-WT and in SRI-D76N upon excitation with 580 nm indicates also that amino acids other than Asp76, and other than those related to the Schiff base, are involved in the process. The difference in structural volume changes as well as differences in the activation parameters for the S610 decay should be attributed to differences in the rigidity of the cavity surrounding the chromophore. Except for the decay of the first intermediate, which is faster than in the SRI-transducer complex, the rate constants of the photocycle for transducer-free SRI in detergent suspension are strongly retarded with respect to wild-type membranes (this comparison should be done with great care because the preparation of both samples is very different).  (+info)

*Electrochemical gradient

The way bacteriorhodopsin generates a proton gradient in Archaea is through a proton pump. The proton pump relies on proton ... In bacteriorhodopsin, the proton pump is activated by absorption of photons of 568 nm wavelength which leads to isomerization ... Finally, bacteriorhodopsin returns to its resting state when Asp85 releases its proton to Glu204.[16][17] ... Diagram of the conformational shift in retinal that initiates proton pumping in bacteriorhodopsin. ...

*Proton pump

Main article: Bacteriorhodopsin. Bacteriorhodopsin is a light-driven proton pump and is used by Archaea, most notably in ... The energy required for the proton pumping reaction may come from light (light energy; bacteriorhodopsins), electron transfer ( ...

*Arkea bahasa Indonesia, ensiklopedia bebas

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. PMID 14977418. doi:10.1146/annurev.physiol.66.032102. ... "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature 406 (6796): 653-7. PMID 10949309. doi: ...

*Archaea

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. doi:10.1146/annurev.physiol.66.032102.150049. PMID ... In the Halobacteria, light-activated ion pumps like bacteriorhodopsin and halorhodopsin generate ion gradients by pumping ions ... "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature. 406 (6796): 653-7. doi:10.1038/35020614. ...

*Proton pump

Bacteriorhodopsin is a light-driven proton pump and is used by Archaea, most notably in Halobacteria. Light is absorbed by a ... The energy required for the proton pumping reaction may come from light (light energy; bacteriorhodopsins), electron transfer ( ...

*Model lipid bilayer

"Unfolding pathways of individual bacteriorhodopsins". Science. 288 (5463): 143-146. Bibcode:2000Sci...288..143O. doi:10.1126/ ...

*Archaea

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. doi:10.1146/annurev.physiol.66.032102.150049. PMID ... Bacteriorhodopsin from Halobacterium salinarum. The retinol cofactor and residues involved in proton transfer are shown as ball ... In the Halobacteria, light-activated ion pumps like bacteriorhodopsin and halorhodopsin generate ion gradients by pumping ions ... Subramaniam S; Henderson R (August 2000). "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature ...

*باستانیان - ویکی‌پدیا، دانشنامهٔ آزاد

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. PMID 14977418. doi:10.1146/annurev.physiol.66.032102. ... Bacteriorhodopsin from Halobacterium salinarum. The retinol cofactor and residues involved in proton transfer are shown as ball ... In the Halobacteria, light-activated ion pumps like bacteriorhodopsin and halorhodopsin generate ion gradients by pumping ions ... Subramaniam S; Henderson R (August 2000). "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature ...

*Lysosomal cystine transporter family

Alignment of bacteriorhodopsins with the yeast sequence YRO2". Pharm Res. 14 (11): 1533-41. PMID 9434271. Molecular and ...

*Proteorhodopsin

... by a mechanism similar to that of bacteriorhodopsin. As in bacteriorhodopsin, the retinal chromophore of proteorhodopsin is ... Bacteriorhodopsin was discovered in 1971 and named in 1973 and is currently only known to exist in the archea domain, not ... Bacteriorhodopsin and Halorhodopsin both only exist in the Archea domain whereas proteorhodopsin spans bacteria, archea, and ... In comparison with its better-known archaeal homolog bacteriorhodopsin, most of the active site residues of known importance to ...

*Microbial rhodopsin

... known today as bacteriorhodopsin. The first bacteriorhodopsin turned out to be of archaeal origin, from Halobacterium salinarum ... There are 9 subfamilies in the MR family: (1) Bacteriorhodopsins pump protons out of the cell; (2) Halorhodopsins pump chloride ... Among the high resolution structures for members of the MR Family are the archaeal proteins, bacteriorhodopsin , sensory ... Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin. Most residues participating ...

*Har Gobind Khorana

After the middle of the 1970s, his lab studied the biochemistry of bacteriorhodopsin, a membrane protein that converts light ... Wildenauer, D.; Khorana, H. G. (1977). "The preparation of lipid-depleted bacteriorhodopsin". Biochimica et Biophysica Acta. ...

*Arqueas, a enciclopedia libre

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. PMID 14977418. doi:10.1146/annurev.physiol.66.032102. ... Subramaniam S, Henderson R (2000). "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature 406 ( ...

*Arqueas, a enciclopedia libre

Lanyi JK (2004). "Bacteriorhodopsin". Annu. Rev. Physiol. 66: 665-88. PMID 14977418. doi:10.1146/annurev.physiol.66.032102. ... Subramaniam S, Henderson R (2000). "Molecular mechanism of vectorial proton translocation by bacteriorhodopsin". Nature 406 ( ...

*Electron crystallography

The first electron crystallographic protein structure to achieve atomic resolution was bacteriorhodopsin, determined by Richard ... "Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy". J Mol Biol. 213 (4): 899-929. ...

*Arheje - Wikipedija, prosta enciklopedija

2004). "Bacteriorhodopsin". Annu Rev Physiol 66: 665-88. PMID 14977418. doi:10.1146/annurev.physiol.66.032102.150049.. ...

*Coherent control

"Coherent control of retinal isomerization in bacteriorhodopsin." Science 313, no. 5791 (2006): 1257-1261. Wohlleben, Wendel, ...

*R. J. Dwayne Miller

"Coherent Control of Retinal Isomerization in Bacteriorhodopsin". Science. 313 (5791): 1257-1261. doi:10.1126/science.1130747. ...

*Photosynthesis

The bacteriorhodopsin changes its configuration in response to sunlight, acting as a proton pump. This produces a proton ...

*Pyroglutamic acid

Pyroglutamate is found in many proteins including bacteriorhodopsin. N-terminal glutamic acid and glutamine residues can ...

*G protein-coupled receptor

Early structural models for GPCRs were based on their weak analogy to bacteriorhodopsin, for which a structure had been ... Pebay-Peyroula E, Rummel G, Rosenbusch JP, Landau EM (September 1997). "X-ray structure of bacteriorhodopsin at 2.5 angstroms ... "Surface of bacteriorhodopsin revealed by high-resolution electron crystallography". Nature. 389 (6647): 206-11. doi:10.1038/ ... the relative orientation of the helices differ significantly from that of bacteriorhodopsin. In 2007, the first structure of a ...

*Site-directed spin labeling

2. Spin labeling of bacteriorhodopsin mutants at unique cysteines". Biochemistry. 28: 7806-7812. doi:10.1021/bi00445a042. ... Spin Labeling of Bacteriorhodopsin Mutants". Science. 248 (4959): 1088-192. Bibcode:1990Sci...248.1088A. doi:10.1126/science. ...

*Halobacteriaceae

Large blooms appear reddish, from the pigment bacteriorhodopsin. This pigment is used to absorb light, which provides energy to ...

*Beggars and Choosers (novel)

They are also infused with bacteriorhodopsin, allowing photosynthesis. It is now possible for a human being to lie on the ...

*Halobacteriales

Large blooms appear reddish, from the pigment bacteriorhodopsin. This pigment is used to absorb light, which provides energy to ...

*Archaerhodopsin

The retinal proteins, bacteriorhodopsin (bR)' and halorhodopsin (hR) of Halobacterium halobium, are light-driven pumps for the ... These proteins are similar to both bacteriorhodopsin and halorhodopsin. They drive the hyperpolarization of the cell membrane ... The detection of retinal protein similar to bacteriorhodopsin in four halobacterium species. Acta Biophysica Sinica (in Chinese ... As previously mentioned archaerhodopsin proteic family differentiates itself from the bacteriorhodopsin (bR) proteins by a 55- ...
TY - JOUR. T1 - Effect of Asp85 replacement by Thr on the conformation, surface electric properties and stability of bacteriorhodopsin. AU - Taneva, Stefka G.. AU - Gõi, Felix M.. AU - Tuparev, Nikolai P.. AU - Petkanchin, Ivana. AU - Dér, A.. AU - Muga, Arturo. PY - 2002/9/25. Y1 - 2002/9/25. N2 - The role of Asp85 in maintaining bacteriorhodopsin structure has been analyzed by infrared spectroscopy, electric light scattering and differential scanning calorimetry. In comparison with the wild type bacteriorhodopsin, the mutant protein D85T shows a different conformation, electric dipole moments and decreased thermal stability. The conformational rearrangements affect both the transmembrane helices and the extramembranous protein segments. Both electric dipoles - the permanent dipole moment and the electric polarizability - have drastically lower values for the membranes containing D85T variant of bacteriorhodopsin. Therefore, this single amino acid mutation not only changes bacteriorhodopsin ...
The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental techniques with a time resolution of 5 × 10-13 s. The following results were obtained. (a) After optical excitation the first excited singlet state S1 of bacteriorhodopsin is observed via its fluorescence and absorption properties. The population of the excited singlet state decays with a lifetime τ1 of ~0.7 ps (430 ± 50 fs) (52). (b) With the same time constant the first ground-state intermediate J builds up. Its absorption spectrum is red-shifted relative to the spectrum of BR by ~30 nm. (c) The second photoproduct K, which appears with a time constant of τ2 = 5 ps shows a red-shift of 20 nm, relative to the peak of BR. Its absorption remains constant for the observation time of 300 ps. (d) Upon suspending bacteriorhodopsin in D2O and deuterating the retinal Schiff base at its nitrogen (lysine 216), the same photoproducts J and K are observed. The relaxation time ...
Biological molecules and assemblies, such as the photochemical reaction center, are capable of capturing light with good quantum efficiency and transforming it into chemical energy. If properly exploited, such assemblies have potential applications as biomolecule information processing units.. Bacteriorhodopsin, from the purple membrane bacterium Halobacterium halobium, is one such system that has been studied extensively and has been commercialized into optical holographic memories (Birge 1995). In the bacterium, the protein bacteriorhodopsin self-assembles into ordered lipid patches. The protein absorbs light and undergoes a cycle involving a complex series of intermediates, resulting in a proton being pumped across the membrane. It was information developed from understanding the basic science behind the way that bacteriorhodopsin works that led to the use of bacteriorhodopsin as a biomolecule information processing unit. To be used for information storage, the protein is placed under ...
The transmembrane electrochemical proton gradient is a key source of cellular energy. Bacteriorhodopsin (bR), the simplest and most studied proton pump, moves protons from low to high concentration by harnessing light energy, creating the transmembrane el
1DZE: Crystal Structure of the M Intermediate of Bacteriorhodopsin: Allosteric Structural Changes Mediated by Sliding Movement of a Transmembrane Helix
3-Fluoro-DL-tyrosine can be used to study how halogenated tyrosines affect proteins such as bacteriorhodopsin, microvillar enzymes, and β-galactosidases.
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Classical planning is the problem of finding a sequence of actions, from a given initial state to some goal state. While, in generalized planning, a plan is a solution to a set of planning problems, which belong to the ...
Bacteriorhodopsins are a large family of seven-helical transmembrane proteins that function as light-driven proton pumps. Here, we present the crystal structure of a new member of the family, Haloarcula marismortui bacteriorhodopsin I (HmBRI) D94N mutant, at the resolution of 2.5 Å. While the HmBRI retinal-binding pocket and proton donor site are similar to those of other archaeal proton pumps, its proton release region is extended and contains additional water molecules. The proteins fold is reinforced by three novel inter-helical hydrogen bonds, two of which result from double substitutions relative to Halobacterium salinarum bacteriorhodopsin and other similar proteins. Despite the expression in Escherichia coli and consequent absence of native lipids, the protein assembles as a trimer in crystals. The unique extended loop between the helices D and E of HmBRI makes contacts with the adjacent protomer and appears to stabilize the interface. Many lipidic hydrophobic tail groups are discernible in the
Biological light-driven proton pumps use light to move protons across a cell membrane, creating a proton gradient. Although photochromic compounds such as spiropyrans can reversibly convert between two structures with differing pKa values, spiropyrans have not been used to generate either a light-driven proton pump or an electrical current. Here, we report an artificial light-harvesting system based on a supported liquid membrane doped with a spiropyran. Irradiating the membrane with ultraviolet light induces a ring-opening reaction, converting spiropyran to merocyanine, whereas irradiation with visible light induces the reverse reaction. When the membrane is irradiated with ultraviolet and visible light on opposite sides, H+ is taken up by merocyanine, carried through the polymeric membrane and released on the other side. We show that this system produces a light-induced proton flux, an electrical current with an efficiency of ~0.12%, an open-circuit voltage of ~210 mV and a membrane gradient of ~3.6
0070]Bacteriorhodopsin (purple membrane) has been incorporated in liposomes using sonication. The lipid mixture used was 4:1 DOPE: EggPC. DOPE has been shown to increase the incorporation of bacteriorhodopsin (bR). 10 mM pH buffer was used so that we could see the change in pH due to bacteriorhodopsin. After gelation, a few drops of buffer were added to the sample if the pH was not stable, for example due to improper contact with the pH electrode. The pH levels of gels containing the bR-incorporated lipsomes before and after light exposure were measured and compared with the pH levels of blank-liposomes in gel before and after light exposure. Light with a yellow light filter was shined for 30 sec and a break for 60 sec was given. This was due to the fact that the blank-liposome gel in buffer was showing a slight decrease in pH, which was suspected to be due to a heat effect. The pH levels of the blank returned to baseline after exposure to light, whereas the pH levels of the bR-incorporated ...
The story behind optogenetics is a story of convergence of two seemingly unrelated fields: microbial microbiology and neuroscience. In 1971, Walther Stoeckenius and Dieter Oesterhelt discovered the transmembrane protein bacteriorhodopsin (BR) that is capable of moving ions in the presence of light. BR is highly expressed in haloarcheal membranes while a similar transmembrane protein proteorhodopsin (PR) is expressed in eubacteria. In 1977, Matsuno-Yagi and Mukohata described the ion pump channel halorhodopsin which displaces extracellular chloride ions in to the cell. As these discoveries were happening in the background, a growing desire to effectively control the neurons was ever -prevalent. During this period, neuroscientist adopted the idea that cognitive processes were distributed in various localizations of the cortex. This fact evident when lesions in specific areas would lead to loss of a specific process. Even fMRIs and PET solidified this point, but, even with these high-tech ...
The story behind optogenetics is a story of convergence of two seemingly unrelated fields: microbial microbiology and neuroscience. In 1971, Walther Stoeckenius and Dieter Oesterhelt discovered the transmembrane protein bacteriorhodopsin (BR) that is capable of moving ions in the presence of light. BR is highly expressed in haloarcheal membranes while a similar transmembrane protein proteorhodopsin (PR) is expressed in eubacteria. In 1977, Matsuno-Yagi and Mukohata described the ion pump channel halorhodopsin which displaces extracellular chloride ions in to the cell. As these discoveries were happening in the background, a growing desire to effectively control the neurons was ever -prevalent. During this period, neuroscientist adopted the idea that cognitive processes were distributed in various localizations of the cortex. This fact evident when lesions in specific areas would lead to loss of a specific process. Even fMRIs and PET solidified this point, but, even with these high-tech ...
1BCT: Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution.
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Parent Directory - phylogenetictree.jpg 20-Jul-2014 00:42 24K phylogenetictree-t.jpg 20-Jul-2014 00:42 7.0K Bacteriorhodopsin/ 20-Jul-2014 00:42 - Archaeacells.htm 20-Jul-2014 00:42 965 ...
Die Erfindung betrifft einen Testträger zur analytischen Bestim mung eines Bestandteils einer Körperflüssigkeit mit einer Basis schicht und mindestens zwei flachen im Ausgangszustand des Test trägers vor Durchführung einer Bestimmung voneinander getrennten, jedoch durch externe Manipulation in Kontakt zueinander bringbaren Testschichten. The invention relates to a test carrier for the analytical Bestim mung a component of a body fluid with a base layer and at least two flat in the initial state of the test carrier prior to making a determination on separate, but mutually by external manipulation can be brought into contact test layers. Während früher im klinischen Labor die Konzentration beispiels weise der Bestandteile des Blutes praktisch ausschließlich mit Hilfe flüssiger Reagenzien bestimmt wurde, haben in jüngerer Zeit sogenannte trägergebundene Tests zunehmend an Bedeutung gewonnen. While earlier in the clinical laboratory, the concentration example, the components of the blood ...
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A group of microbial retinal proteins most closely related to the proton pump xanthorhodopsin has a novel sequence motif and a novel function. Instead of, or in addition to, proton transport, they perform light-driven sodium ion transport, as reported for one representative of this group (KR2) from Krokinobacter. In this paper, we examine a similar protein, GLR from Gillisia limnaea, expressed in Escherichia coli, which shares some properties with KR2 but transports only Na +. The absorption spectrum of GLR is insensitive to Na + at concentrations of ≤3 M. However, very low concentrations of Na + cause profound differences in the decay and rise time of photocycle intermediates, consistent with a switch from a "Na +-independent" to a "Na +-dependent" photocycle (or photocycle branch) at ~60 μM Na +. The rates of photocycle steps in the latter, but not the former, are linearly dependent on Na + concentration. This suggests that a high-affinity Na + binding site is created transiently after ...
In a general description of the various ion transport modes found for halobacterial retinal proteins (Haupts et al., 1997), the transport mechanism is represented by a sequence of six principal reaction steps: retinal isomerization to 13‐cis or all‐trans (either photochemical, designated as I*, or thermal, I), ion translocation to or from the Schiff base (T) and switch of the Schiff base accessibility towards the EC or CP side (S). Within this framework, the anion transport mechanism of wild type HR, taking into consideration the results on specific mutants, can be described as follows (Figure 7): in the initial state, the substrate anion occupies a binding site constituted by R108 and T111, also interacting with the protonated Schiff base (Walter and Braiman, 1994), and the Schiff base is accessible for ions from the EC side. (1) Light‐induced isomerization (I*) is followed by (2) translocation of this anion towards the Schiff base (T), where it is located in the HR520 intermediate state ...
The flagship monthly journal of SPIE, |i|Optical Engineering|/i| (OE) publishes peer-reviewed papers reporting on research and development in all areas of optics, photonics, and imaging science and engineering.
Worcester Polytechnic Institute (WPI) and Quinsigamond Community College (QCC) have been awarded a $4 million grant by the Commonwealth of Massachuset
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A post-doctoral position is available immediately in the laboratory of Dr Joe Cockburn, at the Astbury Centre, University of Leeds, UK to perform structure-function studies on ciliary proteins.. The position is funded by The Wellcome Trust and is available immediately for a period of 24 months. The start date is flexible but must be before September 2017.. The deadline for applications is Monday 16th January 2017.. More information about the position and how to apply can be found here. ...
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Sensing light from the environment using photoreceptors is of great adaptive significance to eukaryotes. A prominent feature of the photochemistry of these receptors is the photocycle length, the time taken to decay from the initial signaling light state back to the receptive dark state. Photoreceptors have distinct photocycle lengths and this suggests evolutionary selection of the photochemistry for biological function. However, the biological significance of the photocycle length is not understood as most in vivo studies with photoreceptors utilize true null or blind photoreceptor mutants. Neurospora crassa provides a fantastic template for studying photobiology and the plethora of molecular and genetic resources available for this organism have enabled us to test the significance of photoreceptor photocycle length of the VIVID (VVD) protein. Using defined in vitro mutations that alter the photocycle length of the VVD protein over 4 orders of magnitude we have been able dissect the functional ...
Sensing light from the environment using photoreceptors is of great adaptive significance to eukaryotes. A prominent feature of the photochemistry of these receptors is the photocycle length, the time taken to decay from the initial signaling light state back to the receptive dark state. Photoreceptors have distinct photocycle lengths and this suggests evolutionary selection of the photochemistry for biological function. However, the biological significance of the photocycle length is not understood as most in vivo studies with photoreceptors utilize true null or blind photoreceptor mutants. Neurospora crassa provides a fantastic template for studying photobiology and the plethora of molecular and genetic resources available for this organism have enabled us to test the significance of photoreceptor photocycle length of the VIVID (VVD) protein. Using defined in vitro mutations that alter the photocycle length of the VVD protein over 4 orders of magnitude we have been able dissect the functional ...
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INTRODUCTION. In a recent report by Nishikawa et al. (1), the microorganisms isolated from the soil exhibited resistance towards benzalkonium chloride. Russel and co-workers have (2) proposed two possible mechanisms for such resistance: the intrinsic and acquired resistances. The intrinsic resistance related to the structural and chemical composition of the outer layers of the cells, which may provide an effective barrier to the entry of antibacterial agents. The acquired resistance results from genetic changes in the bacterial cell and arises either by mutation or by the acquisition of genetic material from another cell. Extensive investigations in the field of Schiff bases have been reported (3-5). Antimicrobial and antifungal activities of various Schiff bases ...
Wang J, Song L, Yoo S-K, EL-Sayed MA. A Comparison of the Photoelectric Current Responses Resulting from the Proton Pumping Process of Bacteriorhodopsin under Pulsed and CW Laser Excitations. The Journal of Physical Chemistry B [Internet]. 1997 ;101(49):10599 - 10604. Available from: http://dx.doi.org/10.1021/jp972475r ...
Wang J, Song L, Yoo S-K, EL-Sayed MA. A Comparison of the Photoelectric Current Responses Resulting from the Proton Pumping Process of Bacteriorhodopsin under Pulsed and CW Laser Excitations. The Journal of Physical Chemistry B [Internet]. 1997 ;101(49):10599 - 10604. Available from: http://dx.doi.org/10.1021/jp972475r ...
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Three positional isomers of a Schiff base containing -OH as end groups have been synthesized and evaluated for selective Al(iii) detection due to inhibition of ESIPT, PET and activation of CHEF in 70% aqueous medium. Devoid of any conventional fluorophore, these sensors have nanomolar detection limits with h
States of the model at step 10 in runs with simulated knock-outs of individual genes.We ran the model in synchronous mode for 10 steps from the initial state an
The model operating point consists of the initial state values and input signals stored in the model. For information on linearizing models at different operating points, see docid:slcontrol_ug#bso7hgx and docid:slcontrol_ug#bso7hhu.. You can then analyze the response of the linearized model. For example, plot its Bode response.. ...
I just checked on two different Intel processors and two different ones from AMD. Summing all sqrt-s from 0 to 15 in increments of 0.001 yields the same results on all 4 processors. Therefore I assume that if you use the same initial state (including the internal state of the library) with floating or fixed point and fixed time-step you will get complete determinism. That is of course granted there are no time-seeded rands, but I think Box2D doesnt ...
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It has been one of those days. I think I could scream until Im purple in the face and this day would not get any better. Not for me, or my husband. He is having a bad day at work today and of course that makes me anxious for him. For me, I have felt…
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pApa: athirAh, bar-ney. athirAh: ba-bi. pApa: bar-ney!. athirAh: ba-bi!. pApa: BAR-NEY!. athirAh: BA-BI!!!. (i reckon athirAh was wondering, "y lar my parents asking me to repeat it again n again, didnt i just pronounce it correctly....?") i then tried my luck.... mOmmy: athirAh, bar-ney!. athirAh: babibabibabibabibabibabi......babibabibabibabibabi (sambil walk away from us...). hhehehe, BARNEY lar sayang, bukan babi.... ...
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Bacteriorhodopsin is a purple protein that is embedded in the membrane of a photosynthesising organism, where it captures the energy from the suns rays. Instead of removing the sensitive protein from the membrane, as Richard Henderson had previously tried to do, he and his colleagues took the complete purple membrane and put it under the electron microscope. In this way, the protein retained its structure because it remained membrane-bound. To prevent the samples surface from drying out in the vacuum, they covered it with a glucose solution.. The harsh electron beam was a major problem, but the researchers made use of the way in which bacteriorhodopsin molecules are packed in the organisms membrane. Instead of blasting it with a full dose of electrons, they used a weaker beam. The images contrast was poor, and they could not see the individual molecules, but they were able to make use of the fact that the proteins were regularly packed and oriented in the same direction. When all the ...
Synthetic biology is a highly interdisciplinary field, which combines biology, chemistry and physics with engineering. Its goal is to design molecular factories and synthetic cells with novel properties or functions for applications in healthcare, industry, or biological and medical research. Such artificial systems are in the nanometer scale and are built by combining and assembling existing, synthetic or engineered building blocks (e.g., proteins). Molecular systems have wide application ranges, e.g., for chemical compound synthesis, waste disposal, energy supply and medical diagnosis or treatment.. In this context, the NCCR Molecular Systems Engineering brings Swiss scientists from different disciplines together to stimulate innovation, and address existing and future challenges. The University of Bern is represented by the Fotiadis laboratory in the NCCR MSE.. Nanomachines for energy conversion. Energy-providing building blocks are essential to power molecular systems. Light-driven proton ...
Participants: 1) Peter Hegemann (PI; Humboldt University Berlin), coworker 2) Benjamin Rost (PI; Charité, Berlin), coworker: Abstract: Development and Application of New Optogenetic Tools Targeted to Intracellular Compartments The aim of our proposed work program is the development of novel optogenetic tools that allow investigations on subcellular compartments. We will combine optogenetic actuators and sensors with targeting motives for synaptic vesicles (SV) or lysosomes. The strategy will be based on our recently published work on optogenetic acidification of SV and lysosomes by organelle-specific expression of the light-driven proton pump Arch3. In order to achieve acute depletion of protons from SV and lysosomes we will use a proton channel derived from Arch3, which has been recently developed by the Hegemann lab by point-directed mutagenesis. For the manipulation of luminal chloride we will implement chloride-conductive channelrhodopsins or light-driven chloride pumps. The tetraspanin ...
Associative memories and processors differ significantly from the serial memories that dominate modern computing technologies. These memories mimic the function of the human brain, in which associative recall is used to search through the entire memory bank simultaneously to match an input data block [93,94]. In application, the memory would return the corresponding data block that matches the input criteria, or would return the closest match if no exact copy is identified. Because of this functional paradigm, associative processors are often considered a target design for the development of true artificial intelligence [95]. Fourier-transform holographic optical loops, which were first proposed by Paek and Psaltis, have long been considered an optimal architecture necessary to realize the implementation of this memory class [84,96-98]. Bunkin et al. [80] had first proposed thin films of BR as a viable medium for the technology owing to the inherent holographic and photochromic properties of the ...
ID SUI1_HALSA Reviewed; 97 AA. AC Q9HME3; DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2001, sequence version 1. DT 25-OCT-2017, entry version 81. DE RecName: Full=Protein translation factor SUI1 homolog {ECO:0000255,HAMAP-Rule:MF_00604}; GN OrderedLocusNames=VNG_2584C; OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OS (Halobacterium halobium). OC Archaea; Euryarchaeota; Halobacteria; Halobacteriales; OC Halobacteriaceae; Halobacterium. OX NCBI_TaxID=64091; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1; RX PubMed=11016950; DOI=10.1073/pnas.190337797; RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., RA Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., RA Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., RA Leithauser B., Keller K., Cruz R., Danson M.J., Hough D.W., RA Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., RA Isenbarger T.A., Peck R.F., ...
to pyschiatrist. three days before her visit to dr bresalier, who is the one she stated she can feel a heavy blob that is falling from the forehead down into her eyes, but you cant see it unless it illuminates and then it has a purple haze that glows and she can see purple and pinkish crystal like structures growing upward from her eye lashes and at times can see blue electrical lights emit., she had seen a dermatologist with her sister present his name was dr. robert finkelstein. his assistanct asked my frield to draw a photo of what she saw, the first one to ever do that. she drew what she could physically see when it illuminates, remember bacteriorhodopsin and other planton illuminate and have fiber optics attached to them. she drew what look like and we know now were quotom dots, and the branch out ones like a spider that open and shut with crystals attached that were on the sides of jawbone going towards the ears. she drew where they make a bridge like configuration around the eyes, ...
A new way has been found to produce solar fuels by developing "synthetic purple membranes." These membranes involve an assembly of lipid nanodiscs, man-made proteins, and semiconducting nanoparticles that, when taken together, can transform sunlight into hydrogen fuel ...
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Photoactive yellow protein (PYP) is a member of the xanthopsin family of eubacterial blue-light photoreceptors. On absorption of light, PYP enters a photocycle that ultimately transduces the energy contained in a light signal into an altered biological response. Nanosecond time-resolved x-ray crystallography was used to determine the structure of the short-lived, red-shifted, intermediate state denoted [pR], which develops within 1 nanosecond after photoelectronic excitation of the chromophore of PYP by absorption of light. The resulting structural model demonstrates that the [pR] state possesses the cis conformation of the 4-hydroxyl cinnamic thioester chromophore, and that the process of trans to cis isomerization is accompanied by the specific formation of new hydrogen bonds that replace those broken upon excitation of the chromophore. Regions of flexibility that compose the chromophore-binding pocket serve to lower the activation energy barrier between the dark state, denoted pG, and [pR], ...
Voltage-gated ion channels mediate electrical excitability of cellular membranes. Re- duced models of the voltage sensor (VS) of Kv channels produce insight into the electrostatic physics underlying the response of the highly positively charged S4 transmembrane domain to changes in membrane potential and other electrostatic parameters. By calculating the partition function computed from the electrostatic energy over translational and/or rotational degrees of freedom, I compute expectations of charge displacement, energetics, probability distributions of translation & rotation and Maxwell stress for arrangements of S4 positively charged residues and S2 & S3 negatively charged counter-charges; these computations can then be compared with experimental results to elucidate the role of various putative atomic level features of the VS. A paddle model (Jiang et al., 2003) is rejected on electrostatic grounds, owing to unfavorable energetics, insufficient charge displacement and excessive Maxwell stress. On
Culture and the preparation of membranes of CHO cell clones stably expressing human GABAB(1b)/rat GABAB(2) were done as described in detail previously (Urwyler et al., 2001). The preparation of rat brain cortical membranes for native receptor assays was also performed as described previously (Olpe et al., 1990).. [35S]GTPγS Assay. The composition of the assay mixtures (in a final volume of 250 μl in 96-well clear-bottom microtiter plates, Isoplates; PerkinElmer Wallac, Gaithersburg, MD) was as follows: 50 mM Tris-HCl buffer, pH 7.7, 10 mM MgCl2, 0.2 mM EGTA, 2 mM CaCl2, 100 mM NaCl, 10 μM guanosine 5′-diphosphate (30 μM with rat cortical membranes; Sigma-Aldrich, St. Louis, MO), 50 μl of the membrane suspension (approximately 10-20 μg of protein), 1.5 mg wheat germ agglutinin-coated SPA beads (Amersham Biosciences UK, Ltd., Little Chalfont, Buckinghamshire, UK), 0.3 nM [35S]GTPγS (ca. 1,000 Ci/mmol, stabilized solution; Amersham Biosciences UK, Ltd.), and the test compounds at the ...
Organic chemists have long recognized the important role that reaction media play in controlling rates, product distributions and stereochemistry. Recently, much effort has been directed towards the use of organized media to modify reaftivity as compared to that in isotropic liquids. Judicious selection of a given organized system for a given application requires sufficient understanding of the properties of the organized media themselves and those of the substrate interactions therein. The multimolecular aggregation of hydrophobic solutes in water could prove to be of immense value to the organic chemist. The aggregation of simple olefinic systems in water would enable photocycloaddition to compete efficiently with the various other modes of dacay of the short-lived excited state. Investigations of a few systems (dimerization of coumarins, stilbenes and alkylcinnamates) in our laboratory have been successful and they bring to light the significance of the hydrophobic effect. One of the most ...
The photosensitising properties of psoralens such as 1 and 2have been generally related to their (2+2) photoaddition onto the pyrimidine bases of DNA. In recent years a number of results have been...
BioAssay record AID 253508 submitted by ChEMBL: Concentration of compound inhibiting Lys103-Asn mutant HIV-1(IIIB) induced cytopathicity in CEM cell culture by 50%.
What is the Period of PURPLE Crying? The Period of PURPLE Crying is an evidence based program that is delivered in three doses (stages) to parents. The program helps parents understand excessive crying and colic in infants.. The Period of PURPLE Crying is normal in a healthy baby however it can be both distressing and frustrating for new parents. The Period of PURPLE Crying helps parents recognise and understand normal crying and provides coping strategies so parents and care givers can keep their babies safe.. If you have not received information about the Period of PURPLE Crying program, contact your maternity provider.. ...
ROM Number 0109 Icon: Full Name Frogger Helmet Chaos Region USA Languages English Release Group Legacy ROM Size 512Mbit...
This figure shows the evolution in time of an initially random mixture of two components. We can see the components gradually accumulating in separate regions. There are two length scales that play a part. Large fluctuations are smoothed out by diffusion. Meanwhile small local fluctuations want to create lots of interfaces between different regions, which is energetically expensive. This results in an optimal size of fluctuation that spreads the quickest i.e. a characteristic transition length. This is determined by the parameter gamma.. The above demonstration shows the evolution of some initial random state for 200 time units. The above demonstration takes the same initial state, but looks at the effect of gamma on the final state after the same amount of time as before. We can see that gamma does have an effect on the width of the regions between phases (in yellow). Theory predicts these regions should scale as √gamma, which is what we observe.. The above final demonstration puts you in ...
p178] This multiplicative difficulty leading to the need for greater and greater precision in assumptions can be illustrated with the following simple exercise concerning the prediction of the movements of billiard balls on a table. I use the example as computed by the mathematician Michael Berry. If you know a set of basic parameters concerning the ball at rest, can compute the resistance of the table (quite elementary), and can gauge the strength of the impact, then it is rather easy to predict what would happen at the first hit. The second impact becomes more complicated, but possible; you need to be more careful Clbout your knowledge of the initial states, and more precision is called for. The problem is that to correctly compute the ninth impact, you need to take into account the gravitational pull of someone standing next to the table (modestly, Berrys computations use a weight of less than 150 pounds). And to compute the fifty-sixth impact, every single elementary particle of the ...
Second-to-last paragraph: It is essential for the mechanist thesis that the mechanical model of the mind shall operate according [270] to mechanical principles, that is, that we can understand the operation of the whole in terms of the operations of its parts, and the operation of each part either shall be determined by its initial state and the construction of the machine, or shall be a random choice between a determinate number of determinate operations. If the mechanist produces a machine which is so complicated that this ceases to hold good of it, then it is no longer a machine for the purposes of our discussion, no matter how it was constructed. We should say, rather, that he had created a mind, in the same sort of sense as we procreate people at present. There would then be two ways of bringing new minds into the world, the traditional way, by begetting children born of women, and a new way by constructing very, very complicated systems of, say, valves {59} and relays. When talking of ...
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Chemical and Process Engineering Research www.iiste.org ISSN 2224-7467 (Paper) ISSN 2225-0913 (Online) Vol.13, 2013 19 Synthesis and Characterization of Comple…
Purple skin spots - I had a bad fall and my lower back locked on me. Now I have purple, reddish spots on skin in the same place its locked. What is that? Bruise? Is it like a bruise? Could be secondary to fall and a bruise, local hematoma. Or have you put heating pad on it or some muscle cream, gel that you reacted to locally?
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How we see things around us without noticing it! We know the peculiar habit of the sunflower. How it moves with the movement of the sun everyday. But if
Halobacterium salinarum ATCC ® 33171D™ Designation: Genomic DNA from Halobacterium salinarum strain NRC 34002 TypeStrain=True Application:
TY - JOUR. T1 - ATP synthesis by the F0F1 ATP synthase from thermophilic Bacillus PS3 reconstituted into liposomes with bacteriorhodopsin. T2 - 2. Relationships between proton motive force and ATP synthesis. AU - Pitard, Bruno. AU - Richard, Peter. AU - Duñach, Mireia. AU - Rigaud, Jean Louis. PY - 1996/2. Y1 - 1996/2. N2 - The correlation between the rate of ATP synthesis and light-induced proton flux was investigated in proteoliposomes reconstituted with bacteriorhodopsin and ATP synthase from thermophilic Bacillus PS3. By variation of the actinic light intensity it was found that ATP synthase activity depended in a sigmoidal manner on the amplitude of the transmembrane light-induced pH gradient. Maximal rates of ATP synthesis (up to 200 nmol ATP · min-1 · mg protein-1 were obtained at saturating light intensities under a steady-state pH gradient of about pH 1.25. It was demonstrated that this was the maximal ΔpH attainable at 40°C in reconstituted proteoliposomes, due to the feedback ...
Membrane proteins are of significant importance, performing a variety of biological functions including pumps, channels, and receptors. Thus, membrane proteins represent attractive candidates as drug targets. Bacteriorhodopsin (bR), the most widely studied membrane protein, consists of seven transmembrane helical segments and functions which can work as a proton pump in Halobacterium Salinarium. In the present study, the reversible control of bR conformation with simple light illumination is examined, providing a protocol to probe membrane protein folding (a challenge even to this day due to the large, aggregation-prone hydrophobic regions of membrane proteins compared to soluble proteins). Two general methodologies are utilized to control membrane protein folding, including (1) saturation of the natural lipids with a photoresponsive surfactant resulting in partitioning of the protein into detergent-lipid mixed micelles in the unfolded state, and (2) the development of artificial bilayers ...
In most organisms biological processes are partitioned, or phased to specific times over the day through interactions between external cycles of temperature (thermocycles) and light (photocycles), and the endogenous circadian clock. This orchestration of biological activities is achieved in part through an underlying transcriptional network. To understand how thermocycles, photocycles and the circadian clock interact to control time of day specific transcript abundance in Arabidopsis thaliana, we conducted four diurnal and three circadian two-day time courses using Affymetrix GeneChips (ATH1). All time courses were carried out with seven-day-old seedlings grown on agar plates under thermocycles (HC, hot/cold) and/or photocycles (LD, light/dark), or continuous conditions (LL, continuous light; DD, continuous dark, HH, continuous hot). Whole seedlings (50-100), including roots, stems and leaves were collected every four hours and frozen in liquid nitrogen. The four time courses interrogating the

bacteriorhodopsin - Everything2.combacteriorhodopsin - Everything2.com

A pigmented protein embedded in the cell membrane of the bacteria Halobacterium halobium and other photosynthetic species of bacteria. The protein trans...
more infohttps://everything2.com/title/bacteriorhodopsin

Bacteriorhodopsin - WikipediaBacteriorhodopsin - Wikipedia

Bacteriorhodopsin is a trimer. Red line indicates extracellular side (EC) of the membrane Bacteriorhodopsin single monomer with ... Bacteriorhodopsin is a protein used by Archaea, most notably by Halobacteria, a class of the Euryarchaeota. It acts as a proton ... Both rhodopsin and bacteriorhodopsin belong to the 7TM receptor family of proteins, but rhodopsin is a G protein-coupled ... The bacteriorhodopsin molecule is purple and is most efficient at absorbing green light (wavelength 500-650 nm, with the ...
more infohttps://en.wikipedia.org/wiki/Bacteriorhodopsin

Unfolding Pathways of Individual Bacteriorhodopsins | ScienceUnfolding Pathways of Individual Bacteriorhodopsins | Science

Unfolding Pathways of Individual Bacteriorhodopsins. By F. Oesterhelt, D. Oesterhelt, M. Pfeiffer, A. Engel, H. E. Gaub, D. J. ... Unfolding Pathways of Individual Bacteriorhodopsins. By F. Oesterhelt, D. Oesterhelt, M. Pfeiffer, A. Engel, H. E. Gaub, D. J. ... We chose bacteriorhodopsin (BR), a light-driven proton pump, because it represents one of the most extensively studied membrane ... Individual bacteriorhodopsin molecules were first localized and then extracted from the membrane; the remaining vacancies were ...
more infohttps://science.sciencemag.org/content/288/5463/143?ijkey=16e260b2f225a033efa95b5d0ffd855f044378e1&keytype2=tf_ipsecsha

1M0M: Bacteriorhodopsin M1 Intermediate At 1.43 A Resolution1M0M: Bacteriorhodopsin M1 Intermediate At 1.43 A Resolution

BACTERIORHODOPSIN1-[2,6,10.14-Tetramethyl-Hexadecan-16-Yl]-2-[2,10,14-Trimethylhexadecan-16-Yl]glycerol2,10,23-Trimethyl-TetracosaneRetinal
more infohttps://www.ncbi.nlm.nih.gov/Structure/pdb/1M0M

Bacteriorhodopsin facts, information, pictures | Encyclopedia.com articles about BacteriorhodopsinBacteriorhodopsin facts, information, pictures | Encyclopedia.com articles about Bacteriorhodopsin

Make research projects and school reports about Bacteriorhodopsin easy with credible articles from our FREE, online ... bacteriorhodopsin A protein pigment found in certain species of bacteria of the genus Halobacterium. Bacteriorhodopsin is ... Bacteriorhodopsin is composed of seven α-helix segments, which span the membrane and are joined together by short amino-acid ... bacteriorhodopsin A Dictionary of Biology © A Dictionary of Biology 2004, originally published by Oxford University Press 2004. ...
more infohttps://www.encyclopedia.com/plants-and-animals/botany/botany-general/bacteriorhodopsin

Bacteriorhodopsin crystals consume their smaller counterparts | EurekAlert! Science NewsBacteriorhodopsin crystals consume their smaller counterparts | EurekAlert! Science News

... and their international colleagues have studied the crystallization of molecules of the membrane protein bacteriorhodopsin. The ... Bacteriorhodopsin crystals consume their smaller counterparts The scientists have demonstrated that the larger crystals of the ... In their paper, the authors studied the growth of crystals of bacteriorhodopsin (which was used as a model protein of any ... Bacteriorhodopsin crystals consume their smaller counterparts. Moscow Institute of Physics and Technology ...
more infohttps://www.eurekalert.org/pub_releases/2015-12/miop-bcc120915.php

All-optical nonlinear holographic correlation using bacteriorhodopsin filmsAll-optical nonlinear holographic correlation using bacteriorhodopsin films

We investigate the effect of this nonlinearity on the result of the correlation process in a bacteriorhodopsin-based DAJFT ... Films made of the halobacterial photochrome bacteriorhodopsin (BR) can be used in a number of holographic real-time ... Ralph Thoma, Michael Dratz, Norbert A. Hampp, "All-optical nonlinear holographic correlation using bacteriorhodopsin films," ... Ralph Thoma, Michael Dratz, Norbert A. Hampp, "All-optical nonlinear holographic correlation using bacteriorhodopsin films," ...
more infohttps://www.spiedigitallibrary.org/journals/Optical-Engineering/volume-34/issue-5/0000/All-optical-nonlinear-holographic-correlation-using-bacteriorhodopsin-films/10.1117/12.201654.short

Structure Cluster 









- 1P8U: BACTERIORHODOPSIN N INTERMEDIATE AT 1.62 A RESOLUTION 3D Similarity Report PageStructure Cluster - 1P8U: BACTERIORHODOPSIN N' INTERMEDIATE AT 1.62 A RESOLUTION 3D Similarity Report Page

Crystallographic Structures of the M and N Intermediates of Bacteriorhodopsin: Assembly of a Hydrogen-Bonded Chain of Water ... Description: Bacteriorhodopsin protein , Length: 249 No structure alignment results are available for 1P8U.A explicitly.. It is ...
more infohttp://www.rcsb.org/pdb/explore/structureCluster.do?structureId=1P8U

The primary steps of photosynthesis in bacteriorhodopsinThe primary steps of photosynthesis in bacteriorhodopsin

Zinth, Wolfgang; Dobler, J.; Franz, F. A.; Kaiser, Wolfgang (1988): The primary steps of photosynthesis in bacteriorhodopsin. ...
more infohttps://epub.ub.uni-muenchen.de/3558/index.html

Perturbation Effects On The Tryptophan Fluorescence In Bacteriorhodopsin | (1989) | Jang |  Publications | SpiePerturbation Effects On The Tryptophan Fluorescence In Bacteriorhodopsin | (1989) | Jang | Publications | Spie

Perturbation Effects On The Tryptophan Fluorescence In Bacteriorhodopsin Author(s): Du-Jeon Jang; M. A. El-Sayed ... The picosecond fluorescence kinetics of tryptophan residues in bacteriorhodopsin (bR) and some perturbed analogues are measured ...
more infohttps://spie.org/Publications/Proceedings/Paper/10.1117/12.951654

Singular value decomposition with self-modeling applied to determine bacteriorhodopsin intermediate spectra: Analysis of...Singular value decomposition with self-modeling applied to determine bacteriorhodopsin intermediate spectra: Analysis of...

bacteriorhodopsin;. PCA,. principal component analysis;. PCR,. photocycling ratio;. SM,. self-modeling;. SP,. stoichiometric ... On light excitation, bacteriorhodopsin (BR), the light-driven proton pump in the cell membrane of Halobacterium salinarium, ... Stoichiometric constraints, valid until the onset of the recovery of bleached bacteriorhodopsin at the end of the photocycle, ... Intermediate spectra and photocycle kinetics of the Asp96 -, Asn mutant bacteriorhodopsin determined by singular value ...
more infohttps://www.pnas.org/content/96/8/4408

Time-Resolved Ultraviolet
Resonance Raman of Protein
Structural Changes
in The KI-Intermediate
Of BacteriorhodopsinTime-Resolved Ultraviolet Resonance Raman of Protein Structural Changes in The KI-Intermediate Of Bacteriorhodopsin

Time-Resolved Ultraviolet Resonance Raman of Protein Structural Changes in The KI-Intermediate Of Bacteriorhodopsin. Shoji ... spectra of transient intermediates in the bacteriorhodopsin (BR) photocycle, we developed a new UVRR spectrometer. A home-made ...
more infohttps://www.hindawi.com/journals/lc/1999/093486/abs/

Microwave-Induced Structural Changes in Bacteriorhodopsin: Studies by Optical and Fourier Transform Infrared Difference...Microwave-Induced Structural Changes in Bacteriorhodopsin: Studies by Optical and Fourier Transform Infrared Difference...

... microwave radiation was found to affect the bacteriorhodopsin (BR) structure in films at a 30% relative humidity.... ... Keywords: bacteriorhodopsin dark-light adaptation optical spectroscopy FTIR difference spectroscopy microwave radiation ... Microwave-Induced Structural Changes in Bacteriorhodopsin: Studies by Optical and Fourier Transform Infrared Difference ... microwave radiation was found to affect the bacteriorhodopsin (BR) structure in films at a 30% relative humidity. This study is ...
more infohttps://link.springer.com/article/10.1134%2FS0006350918050226

RCSB PDB 









- 1DZE: Structure of the M Intermediate of Bacteriorhodopsin trapped at 100K Macromolecule Annotations PageRCSB PDB - 1DZE: Structure of the M Intermediate of Bacteriorhodopsin trapped at 100K Macromolecule Annotations Page

Crystal Structure of the M Intermediate of Bacteriorhodopsin: Allosteric Structural Changes Mediated by Sliding Movement of a ...
more infohttp://www.rcsb.org/pdb/explore/derivedData.do?structureId=1DZE

Early picosecond events in the photo cycle of BacteriorhodopsinEarly picosecond events in the photo cycle of Bacteriorhodopsin

d) Upon suspending bacteriorhodopsin in D2O and deuterating the retinal Schiff base at its nitrogen (lysine 216), the same ... a) After optical excitation the first excited singlet state S1 of bacteriorhodopsin is observed via its fluorescence and ... The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental ... Early picosecond events in the photo cycle of Bacteriorhodopsin. In: Biophysical Journal, Nr. 46: S. 651-662 ...
more infohttps://epub.ub.uni-muenchen.de/3299/index.html

Bacteriorhodopsin as a chemical and biological sensor | (2003) | Heeg |  Publications | SpieBacteriorhodopsin as a chemical and biological sensor | (2003) | Heeg | Publications | Spie

Bacteriorhodopsin as a chemical and biological sensor Author(s): Bauke Heeg; Richard Needleman; Anatoliy Khizhnyak; Drew M. ... Bacteriorhodopsin (bR) is a small protein containing the chromophore retinal, and resides in the membrane of the Halobacterium ...
more infohttp://spie.org/Publications/Proceedings/Paper/10.1117/12.488595

Directed evolution of bacteriorhodopsin for applications in bioelectronics | Journal of The Royal Society InterfaceDirected evolution of bacteriorhodopsin for applications in bioelectronics | Journal of The Royal Society Interface

1992 Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its ... 1975 Bacteriorhodopsin: a trans-membrane pump containing α-helix. J. Mol. Biol. 93, 139-142. doi:10.1016/0022-2836(75)90124-2 ( ... 2001 Bacteriorhodopsin. Curr. Opin. Struct. Biol. 11, 415-419. doi:10.1016/S0959-440X(00)00226-8 (doi:10.1016/S0959-440X(00) ... 1975 Bacteriorhodopsin: a light-driven proton pump in Halobacterium halobium. Biophys. J. 15, 955-962. doi:10.1016/S0006-3495( ...
more infohttp://rsif.royalsocietypublishing.org/content/10/84/20130197

7TM Helix Protein Rhodopsin and Bacteriorhodopsin Comparison7TM Helix Protein Rhodopsin and Bacteriorhodopsin Comparison

Compare and contrast the 7TM helix proteins rhodopsin and bacteriorhodopsin at the molecular and cellular levels. Rhodopsin and ... 7TM Helix Protein Rhodopsin and Bacteriorhodopsin Comparison. Print Reference this Disclaimer: This work has been submitted by ... Bacteriorhodopsin is a prokaryotic protein present in archaea. It is a light-dependent proton pump which is used to carry out ... Bacteriorhodopsin is formed of the protein bacterio-opsin which is bound to all-trans-retinal in a covalent and reversible ...
more infohttps://www.ukessays.com/essays/sciences/7tm-helix-protein-rhodopsin-bacteriorhodopsin-5144.php

OSA | Rigorous analysis of the propagation of sinusoidal pulses in bacteriorhodopsin filmsOSA | Rigorous analysis of the propagation of sinusoidal pulses in bacteriorhodopsin films

"Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants ... "Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants ... "Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants ... "Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants ...
more infohttps://www.osapublishing.org/oe/abstract.cfm?uri=oe-20-23-25497

Hidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers | JILA ScienceHidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers | JILA Science

Hidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers. Event Details. Event Dates: Tuesday, ... to reexamine the unfolding of the model membrane protein bacteriorhodopsin. Numerous new intermediates were detected, with many ...
more infohttps://jila.colorado.edu/events/2018/hidden-dynamics-unfolding

Hidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers | JILA ScienceHidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers | JILA Science

Hidden dynamics in the unfolding of bacteriorhodopsin revealed by ultrafast cantilevers. Event Details. Event Dates: Tuesday, ... to reexamine the unfolding of the model membrane protein bacteriorhodopsin. Numerous new intermediates were detected, with many ...
more infohttps://jila.colorado.edu/node/76082

OSA | Molecular dynamics of primary photoprocesses in bacteriorhodopsin: subpicosecond study of absorption and luminescence...OSA | Molecular dynamics of primary photoprocesses in bacteriorhodopsin: subpicosecond study of absorption and luminescence...

Phenomenological characterization of bacteriorhodopsin-D85N photocycle Doğan A. Timuçin and John D. Downie. J. Opt. Soc. Am. B ... Elements of a unique bacteriorhodopsin neural network architecture Dan Haronian and Aaron Lewis. Appl. Opt. 30(5) 597-608 (1991 ... Monolithically integrated bacteriorhodopsin-GaAs field-effect transistor photoreceiver Pallab Bhattacharya, Jian Xu, Gyorgy ... With the help of a streak camera, the luminescence time of bacteriorhodopsin has been measured to be ≤ 2 psec. ...
more infohttps://www.osapublishing.org/josab/abstract.cfm?uri=josab-2-4-634

Evaluation of Immobilized Bacteriorhodopsins Function by Laser IrridiationEvaluation of Immobilized Bacteriorhodopsin's Function by Laser Irridiation

Bacteriorhodopsin (BR) is a retinal protein that is a light-driven proton pump and has an important role in photosynthesis in ... Evaluation of Immobilized Bacteriorhodopsins Function by Laser Irridiation. Article 6, Volume 1, Issue 2, Autumn 2015, Page ... Bacteriorhodopsin (BR) is a retinal protein that is a light-driven proton pump and has an important role in photosynthesis in ... 17] L.M. Shamansky, K.M. Luong, D. Han, E.L. Chroniser, Photohnduced Kinetics of Bacteriorhodopsin in a Dried Xerogel Glass. ...
more infohttp://www.bmmj.org/article_18686.html

Combined QM/MM study of the opsin shift in bacteriorhodopsin - Semantic ScholarCombined QM/MM study of the opsin shift in bacteriorhodopsin - Semantic Scholar

... calculations and molecular dynamics simulations of bacteriorhodopsin (bR) in the membrane matrix have been carried out to ... Quantum Chemistry of in situ Retinal : Study of the Spectral Properties and Dark Adaptation of Bacteriorhodopsin †. *Ilya ... Combined QM/MM study of the opsin shift in bacteriorhodopsin. @article{Rajamani2002CombinedQS, title={Combined QM/MM study of ... Full-Quantum chemical calculation of the absorption maximum of bacteriorhodopsin: a comprehensive analysis of the amino acid ...
more infohttps://www.semanticscholar.org/paper/Combined-QM%2FMM-study-of-the-opsin-shift-in-Rajamani-Gao/fd675e20b84b0f6d0f1db12f0216c652c6ea3882

Molecular Mechanism of Long-range Proton Translocation by BacteriorhodopsinMolecular Mechanism of Long-range Proton Translocation by Bacteriorhodopsin

Bacteriorhodopsin (bR), the simplest and most studied proton pump, moves protons from low to high concentration by harnessing ... Bacteriorhodopsin (bR), the simplest and most studied proton pump, moves protons from low to high concentration by harnessing ... Proton transfers in the bacteriorhodopsin photocycle. Biochimica et biophysica acta 1757(8): 1012-1018.. Song YF, Mao JJ, ... Molecular Mechanism of Long-range Proton Translocation by Bacteriorhodopsin. The transmembrane electrochemical proton gradient ...
more infohttp://www.jbsdonline.com/molecular-mechanism-long-range-proton-translocation-bacteriorhodopsin-p18792.html
  • It was demonstrated that this was the maximal ΔpH attainable at 40°C in reconstituted proteoliposomes, due to the feedback inhibition of bacteriorhodopsin by the proton gradient it generates. (vtt.fi)
  • The hydration and dynamics of purple membranes (PM) containing the bacteriorhodopsin (BR) triple mutant D96G/F171C/F219L were investigated by neutron diffraction coupled with H(2)O/D(2)O exchange and by energy-resolved neutron scattering. (mysciencework.com)
  • Both electric dipoles - the permanent dipole moment and the electric polarizability - have drastically lower values for the membranes containing D85T variant of bacteriorhodopsin. (elsevier.com)
  • The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental techniques with a time resolution of 5 × 10-13 s. (uni-muenchen.de)
  • These observations were interpreted as indicating that, in bacteriorhodopsin/ATP synthase, proteoliposomes, a localized pathway for coupling light-driven H + transport by bacteriorhodopsin to ATP synthesis by F 0 F 1 might exist under specific experimental conditions. (vtt.fi)
  • The experiments characterize the development of all the stages of the initial conformation of bacteriorhodopsin (BR) from the excited state BR* through primary photoproduct J to the intermediate K . (osapublishing.org)
  • The objective of this program is to characterize the two-dimensional crystalline structure of bacteriorhodopsin (BR) as a template into which various nonbiological chromophores can be intercalated. (sbir.gov)
  • I.V. Kalaidzidis, A.N. Kaulen Ad Fau-Radionov, L.V. Radionov An Fau-Khitrina, L.V. Khitrina, Photoelectrochemical Cycle of Bacteriorhodopsin. (bmmj.org)