Bacteriophage T4
Bacteriophage T7
T-Phages
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
Bacteriophage T3
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bacteriophage lambda
Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Bacteriophage mu
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Mutation
Base Sequence
Bacteriophage phi X 174
Bacteriophage phi 6
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
DNA Primase
Viral Tail Proteins
Bacteriophage M13
DNA-Directed DNA Polymerase
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
Bacteriophage P2
Genes
DNA, Single-Stranded
Genetics, Microbial
Siphoviridae
Bacteriolysis
Bacteriophage Typing
Plasmids
Bacteriophage P1
Recombination, Genetic
Polynucleotide Ligases
DNA Helicases
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
DNA Packaging
Salmonella Phages
Virus Replication
Amino Acid Sequence
Centrifugation, Density Gradient
RNA Phages
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Adsorption
Nucleic Acid Conformation
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Bacteriophage PRD1
Pseudomonas Phages
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Templates, Genetic
RNA Nucleotidyltransferases
Cloning, Molecular
Transcription, Genetic
Bacillus Phages
Thymine Nucleotides
Genetic Complementation Test
Exonucleases
Phosphorus Isotopes
Temperature
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Muramidase
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
RNA Ligase (ATP)
Endodeoxyribonucleases
Radiation Effects
Levivirus
Chromosome Mapping
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Oligoribonucleotides
DNA Nucleotidyltransferases
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Site-Specific DNA-Methyltransferase (Adenine-Specific)
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.
Binding Sites
Electrophoresis, Polyacrylamide Gel
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Models, Molecular
Prophages
Electrophoresis, Agar Gel
Transduction, Genetic
Inovirus
Endonucleases
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Deoxyribonucleotides
Suppression, Genetic
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Genetic Code
DNA-Binding Proteins
Attachment Sites, Microbiological
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Protein Binding
DNA, Recombinant
Cryoelectron Microscopy
Substrate Specificity
Operon
Promoter Regions, Genetic
Restriction Mapping
F Factor
Protein Biosynthesis
Nucleotides
Polynucleotide 5'-Hydroxyl-Kinase
Viral Interference
DNA, Circular
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Transferases
Transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme "donor:acceptor group transferase". (Enzyme Nomenclature, 1992) EC 2.
Gene Expression Regulation, Viral
Phosphorus Radioisotopes
Rifampin
A semisynthetic antibiotic produced from Streptomyces mediterranei. It has a broad antibacterial spectrum, including activity against several forms of Mycobacterium. In susceptible organisms it inhibits DNA-dependent RNA polymerase activity by forming a stable complex with the enzyme. It thus suppresses the initiation of RNA synthesis. Rifampin is bactericidal, and acts on both intracellular and extracellular organisms. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1160)
Receptors, Virus
Centrifugation, Zonal
DNA Repair
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Exodeoxyribonucleases
Cystoviridae
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
DNA Ligases
Virus Assembly
Bacteriophage Pf1
Sucrose
DCMP Deaminase
Oligodeoxyribonucleotides
Open Reading Frames
Oligonucleotides
Ribonucleoside Diphosphate Reductase
RNA, Bacterial
Caudovirales
Chromosomes, Bacterial
Colicins
Crosses, Genetic
Thioredoxins
Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.
Exodeoxyribonuclease V
DNA Primers
Adenosine Triphosphatases
Aminacrine
Sequence Analysis, DNA
Adenosine Triphosphate
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Magnesium
Macromolecular Substances
Genes, Regulator
Rho Factor
Crystallography, X-Ray
Sequence Homology, Amino Acid
Deoxyribonuclease (Pyrimidine Dimer)
Sequence Homology, Nucleic Acid
Morphogenesis
Ribonucleotides
Chromatography, DEAE-Cellulose
Virion
Cesium
Mutagenesis
RNA, Transfer
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Biological Therapy
Drug Resistance, Microbial
Host Specificity
Deoxycytidine Monophosphate
Enzyme Induction
Structure-Activity Relationship
Myoviridae
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Viral Regulatory and Accessory Proteins
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
Sodium Dodecyl Sulfate
Phosphotungstic Acid
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
Micrococcus
Mutagenesis, Site-Directed
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Efficient synthesis of nucleic acids heavily modified with non-canonical ribose 2'-groups using a mutantT7 RNA polymerase (RNAP). (1/547)
A T7 RNAP mutant (Y639F) which eliminates discrimination of the chemical character of the NTP ribose 2'-group, facilitates incorporation of non-canonicalsubstrates into nucleic acids. However, transcripts containing a high percentage of non-canonical NMPs are poorly extended due to effects of the 2'-substituents on the transcript:template hybrid conformation. We tested the addition of compounds that stabilize A-type helix geometry to the reaction. High concentrations of polyamines, together with other changes in reaction conditions, greatly increased the synthesis of transcripts heavily substituted with non-canonical ribose 2'-groups. Template structures that facilitate promoter opening increased the efficiency of reactions where non-canonical substrates were incorporated during transcription of +1 to +6. (+info)Comparison of synonymous codon distribution patterns of bacteriophage and host genomes. (2/547)
Synonymous codon usage patterns of bacteriophage and host genomes were compared. Two indexes, G + C base composition of a gene (fgc) and fraction of translationally optimal codons of the gene (fop), were used in the comparison. Synonymous codon usage data of all the coding sequences on a genome are represented as a cloud of points in the plane of fop vs. fgc. The Escherichia coli coding sequences appear to exhibit two phases, "rising" and "flat" phases. Genes that are essential for survival and are thought to be native are located in the flat phase, while foreign-type genes from prophages and transposons are found in the rising phase with a slope of nearly unity in the fgc vs. fop plot. Synonymous codon distribution patterns of genes from temperate phages P4, P2, N15 and lambda are similar to the pattern of E. coli rising phase genes. In contrast, genes from the virulent phage T7 or T4, for which a phage-encoded DNA polymerase is identified, fall in a linear curve with a slope of nearly zero in the fop vs. fgc plane. These results may suggest that the G + C contents for T7, T4 and E. coli flat phase genes are subject to the directional mutation pressure and are determined by the DNA polymerase used in the replication. There is significant variation in the fop values of the phage genes, suggesting an adjustment to gene expression level. Similar analyses of codon distribution patterns were carried out for Haemophilus influenzae, Bacillus subtilis, Mycobacterium tuberculosis and their phages with complete genomic sequences available. (+info)The Cys4 zinc finger of bacteriophage T7 primase in sequence-specific single-stranded DNA recognition. (3/547)
Bacteriophage T7 DNA primase recognizes 5'-GTC-3' in single-stranded DNA. The primase contains a single Cys4 zinc-binding motif that is essential for recognition. Biochemical and mutagenic analyses suggest that the Cys4 motif contacts cytosine of 5'-GTC-3' and may also contribute to thymine recognition. Residues His33 and Asp31 are critical for these interactions. Biochemical analysis also reveals that T7 primase selectively binds CTP in the absence of DNA. We propose that bound CTP selects the remaining base G, of 5'-GTC-3', by base pairing. Our deduced mechanism for recognition of ssDNA by Cys4 motifs bears little resemblance to the recognition of trinucleotides of double-stranded DNA by Cys2His2 zinc fingers. (+info)Co-expression of gene 31 and 23 products of bacteriophage T4. (4/547)
Folding of the major capsid protein of bacteriophage T4 encoded by gene 23 is aided by Escherichia coli GroEL chaperonin and phage co-chaperonin gp31. In the absence of gene product (gp) 31, aggregates of recombinant gp23 accumulate in the cell similar to inclusion bodies. These aggregates can be solubilized with 6 M urea. However, the protein cannot form regular structures in solution. A system of co-expression of gp31 and gp23 under the control of phage T7 promoter in E. coli cells has been constructed. Folding of entire-length gp23 (534 amino acid residues) in this system results in the correctly folded recombinant gp23, which forms long regular structures (polyheads) in the cell. (+info)The environment of 5S rRNA in the ribosome: cross-links to 23S rRNA from sites within helices II and III of the 5S molecule. (5/547)
Three contiguous fragments of Escherichia coli 5S rRNA were prepared by T7 transcription from synthetic DNA templates. The central fragment, comprising residues 33-71 of the molecule, was transcribed in the presence of 4-thiouridine triphosphate together with [32P]UTP. The three transcripts were ligated together, yielding a 5S rRNA analogue carrying 4-thiouridine residues at positions 40, 48, 55 and 65 in helices II and III. After ligation, the 4-thiouridine residues were derivatised with p -azidophenacyl bromide. The modified 5S rRNA was reconstituted into 50S subunits and these subunits were used to prepare 70S ribosomes in the presence or absence of tRNA and mRNA. The azidophenyl groups were then photoactivated by mild irradiation at 300 nm and the products of cross-linking analysed by our standard procedures. Multiple cross-links from 5S rRNA to two distinct regions of the 23S rRNA were observed. The first region was located in helix 38 in Domain II of the 23S molecule, with cross-links at sites between nucleotides 885 and 922. The second region covered helices 81-85 in Domain V, with sites between nucleotides 2272 and 2345. Taken together with previous data, these results serve to define the arrangement of the 5S rRNA molecule relative to the 23S rRNA within the 50S subunit. (+info)RNA polymerase-specific nucleosome disruption by transcription in vivo. (6/547)
The nucleosomal chromatin structure within genes is disrupted upon transcription by RNA polymerase II. To determine whether this disruption is caused by transcription per se as opposed to the RNA polymerase source, we engineered the yeast chromosomal HSP82 gene to be exclusively transcribed by bacteriophage T7 RNA polymerase in vivo. Interestingly, we found that a fraction of the T7-generated transcripts were 3' end processed and polyadenylated at or near the 3' ends of the hsp82 and the immediately downstream CIN2 genes. Surprisingly, the nucleosomal structure of the T7-transcribed hsp82 gene remained intact, in marked contrast to the disrupted structure generated by much weaker, basal level transcription of the wild type gene by RNA polymerase II under non-heat shock conditions. Therefore, disruption of chromatin structure by transcription is dependent on the RNA polymerase source. We propose that the observed RNA polymerase dependence for transcription-induced nucleosome disruption may be related either to the differential recruitment of chromatin remodeling complexes, the rates of histone octamer translocation and nucleosome reformation during polymerase traversal, and/or the degree of transient torsional stress generated by the elongating polymerase. (+info)Vaccinia virus-bacteriophage T7 expression vector for complementation analysis of late gene processes. (7/547)
A vaccinia virus-bacteriophage T7 RNA polymerase hybrid transient expression vector has been developed for complementation analysis of late gene functions in vaccinia virus. The conditionally defective virus ts21 was modified to express the bacteriophage T7 RNA polymerase. The derived virus, vtsT7, was conditionally defective in viral late gene expression but produced high levels of a target protein under the control of a T7 promoter at non-permissive temperatures. The level of beta-galactosidase expression under the control of a T7 promoter was slightly lower in vtsT7 infections than those with the prototypical T7 RNA polymerase vector vTF7.3. However, the levels of expression for the human immunodeficiency virus envelope gene, a protein which undergoes post-translational modification, was slightly higher in vtsT7 infections, suggesting that some proteins may be expressed better in the absence of vaccinia virus late gene expression. Infections using vtsT7 at a low m.o.i. at 39 degrees C resulted in the accumulation of high molecular mass, non-linear replicative intermediates of vaccinia virus DNA replication and high levels of expression of a transfected gene proximal to a T7 promoter. The virus vtsT7 provides a means for the analysis of potential trans-acting factors participating in vaccinia virus late processes such as resolution of DNA replicative intermediates. (+info)Preparation of HIV TAR RNA with RNA scissors. (8/547)
Two hammerhead ribozymes derived from plant pathogenic RNAs were used to cut off the HIV TAR RNA from the T7 RNA transcript through a cis cleavage reaction. Stem I of the (+)vLTSV ribozyme comprises 8 nucleotides of the 5' terminus of TAR RNA, but stem III of the (+)sTRSV ribozyme consists of 8 nucleotides of the 3' end of TAR RNA. The construct containing two GUC hammerhead ribozyme target sequences identified the cleavage sites to cut off a required RNA molecule. This method was applied for preparation of 35 nt long TAR RNA. Its activity was proved by the complex formation with the Tat protein. It seems that this approach based on RNA scissors can also be used for the generation of required RNA molecules, RNA decoys or RNA aptamers in vivo. (+info)
Summer Camps in Ashland City, TN - MySummerCamps
A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity. | Harvard Catalyst...
Bacteriophage T5 Structure Reveals Similarities with HK97 and T4 Suggesting Evolutionary Relationships, Journal of Molecular...
Sequence Similarity
- 1DYA: DETERMINATION OF ALPHA-HELIX PROPENSITY WITHIN THE CONTEXT OF A FOLDED PROTEIN: SITES 44...
Sequence Similarity
- 1DYB: DETERMINATION OF ALPHA-HELIX PROPENSITY WITHIN THE CONTEXT OF A FOLDED PROTEIN: SITES 44...
Identification of P48 and P54 as components of bacteriophage T4 baseplates. | Journal of Virology
Use of UV-irradiated bacteriophage T6 to kill extracellular bacteria in tissue culture infectivity assays<...
Bacteriophage T4 viruses, illustration - Stock Image C024/7526 - Science Photo Library
Use of bacteriophage T7 displayed peptides for determination of monoclonalantibody specificity and biosensor analysis of the...
Recognition and Specific Degradation of Bacteriophage T4 mRNAs | Genetics
Bacteriophage T7-like, protein 6.7 (IPR020134) | InterPro | EMBL-EBI
REGULATION OF A BACTERIOPHAGE T4 LATE GENE, soc, WHICH MAPS IN AN EARLY REGION | Genetics
Tabasco Tom | The Blues Train
OCR (Text recognition) | PixEdit
Polbase - Results for Reference: Amino acid changes coded by bacteriophage T4 DNA polymerase mutator mutants. Relating...
Bacteriophage T4 lysozyme molecule - Stock Image F006/9216 - Science Photo Library
Sambo Medical
부경대학교 미생물학과
Bacteriophage T4 | Harvard Catalyst Profiles | Harvard Catalyst
New Modes of Bacteriophage Spread are Discovered
Gentaur Molecular :bio-gentaur \ MS2 Bacteriophage \ GEN0810066
Tabasco, el edén deforestado
DNA Experiments - WriteWork
Mosbys® Radiography Online for Bontragers Textbook of Radiographic Positioning & Related Anatomy (Access Code), 10th Edition ...
Cynthia Cutts in Akron AL Get a Free Background Report
Repetitive lagging strand DNA synthesis by the bacteriophage T4 replisome<...
Domain effects on the DNA-interactive properties of bacteriophage T4 gene 32 protein | UWF Institutional Repository
Propagating the missing bacteriophages: a large bacteriophage in a new class | Springer for Research & Development
Enterobacteria phage T4 SegA protein
Summary Report | CureHunter
Science Source Stock Photo - Bacteriophage Illustration
Bacteriophages in Human Disease: Friends and Foes | Carolina.com
Researchers Use SARS-CoV-2 to Bioengineer Universal Vaccine Platform | STATNANO
Biology-Online • View topic - bacteriophages
Sandwalk: Green T4 Bacteriophage Earrings
Rocketbook Ocr Doesnt Work - Best Rocketbook to Buy 2020
Escherichia coli bacteriophage T4 ATCC ® 11303-B4™
Transcriptional inhibition of the bacteriophage T7 early promoter region by oligonucleotide triple helix formation
Difference between revisions of TABASCO - OpenWetWare
Polbase - Reference: Protein-protein and protein-DNA interactions at the bacteriophage T4 DNA replication fork....
Bacteriophage Lysogeny
Can Mismatch Endonuclease I be inactivated? | NEB
BiO BB] Help with finding GC rich regions in Bacteriophage genomes
Idealized geometries of bacteriophage - Bacteriophage - BNID 111602
Bacteriophages provide promising results against multidrug-resistant E.coli - Infectious Disease Hub
fd/M13 bacteriophage Antibody (NB100-1633): Novus Biologicals
AllAboutFeed - Bacteriophage - new technology from an ancient source
Bacteriophages Page 1
Bacteriophages Page 2
Pechucalco 2a. Sección (Cunduacán, Tabasco, Mexico) - Population Statistics, Charts, Map, Location, Weather and Web Information
TextGrabber 6 - Real-Time OCR on the App Store
Coriolus Versicolor Extract
Synthetic biology
Chan, Leon Y.; Kosuri, Sriram; Endy, Drew (2005). "Refactoring bacteriophage T7". Molecular Systems Biology. 1: 2005.0018. doi: ... In 2003 the 5386 bp genome of the bacteriophage Phi X 174 was assembled in about two weeks. In 2006, the same team, at the J. ... examples of refactoring including the nitrogen fixation cluster and type III secretion system along with bacteriophages T7 and ... phiX174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences of the United States of ...
Interactome
Some published virus interactomes include Bacteriophage Escherichia coli bacteriophage lambda Escherichia coli bacteriophage T7 ... Bartel PL, Roecklein JA, SenGupta D, Fields S (1996). "A protein linkage map of Escherichia coli bacteriophage T7". Nat. Genet ... 2011). "The protein interaction map of bacteriophage lambda". BMC Microbiol. 11: 213. doi:10.1186/1471-2180-11-213. PMC 3224144 ... Streptococcus pneumoniae bacteriophage Dp-1 Streptococcus pneumoniae bacteriophage Cp-1 The lambda and VZV interactomes are not ...
Processivity
Tabor, S; Richardson, C. C. (1987). "DNA sequence analysis with a modified bacteriophage T7 DNA polymerase". Proceedings of the ... "The thioredoxin binding domain of bacteriophage T7 DNA polymerase confers processivity on Escherichia coli DNA polymerase I". ...
Shirleen Roeder
Roeder, Glenna Shirleen (1978). Recombination, maturation and packaging of the bacteriophage T7 chromosome (Thesis). Toronto: [ ...
Charles C. Richardson
In 1998, Richardson examined the crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution. Before ... Richardson used the T7 RNA polymerase/promoter system to control the expression of a phage T7 gene 5 protein (gp5), which is a ... Mark, D. F.; Richardson, C. C. (March 1, 1976). "Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase". ... Crampton, Donald J.; Richardson, Charles C. (January 1, 2003). "Bacteriophage T7 gene 4 protein: A hexameric DNA helicase". In ...
Myricetin
In T7 bacteriophages myricetin competitively inhibited DNA template binding to RNA polymerase. Myricetin has been seen to ...
LacUV5
Pribnow, D (1975). "Bacteriophage T7 Early Promoters: Nucleotide Sequences of Two RNA Polymerase Binding Sites". Journal of ... "Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes". Journal of Molecular Biology ... and as a result driving the T7 RNA polymerase instead). The two important mutations are underlined. lacUV5 ...
Phikmvvirus
2003). "The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa". Virology. 312 (1): 49-59. doi: ... Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like ... There are currently 16 species in this genus including the type species Pseudomonas virus phiKMV.Bacteriophage phiKMV and its ... of bacteriophage genomes". Journal of Microbiological Methods. 77 (2): 207-13. doi:10.1016/j.mimet.2009.02.006. PMID 19232531. ...
Drew Endy
Endy, Andrew David (1997). Development and application of a genetically-structured simulation for bacteriophage T7 (PhD thesis ... A genetically structured simulation for bacteriophage T7". Biotechnology and Bioengineering. 55 (2): 375-389. doi:10.1002/(SICI ... Endy received his PhD from Dartmouth College in 1997 for his work on genetic engineering using T7 phage. Endy was a junior ...
RNA polymerase
Perhaps the most widely studied such single-subunit RNAP is bacteriophage T7 RNA polymerase. ssRNAPs cannot proofread. B. ...
Antimicrobial properties of copper
"Roles of Copper and Superoxide Anion Radicals in the Radiation-Induced Inactivation of T7 Bacteriophage". Radiat. Res. 99 (3): ...
Escherichia virus T3
FRASER, D; WILLIAMS, RC (Feb 1953). "Details of frozen-dried T3 and T7 bacteriophages as shown by electron microscopy". Journal ... Escherichia virus T3, also called bacteriophage T3 and T3 phage, is a bacteriophage capable of infecting susceptible bacterial ... This phage is closely related to T7 phage in structure though the two viruses may differ in capsid maturation. ... "DNA packaging-associated hyper-capsid expansion of bacteriophage t3". Journal of Molecular Biology. 397 (2): 361-74. doi: ...
Group I catalytic intron
Bonocora RP, Shub DA (December 2004). "A self-splicing group I intron in DNA polymerase genes of T7-like bacteriophages". J. ... T-even and T7-like bacteriophages. Both intron-early and intron-late theories have found evidences in explaining the origin of ... Lee CN, Lin JW, Weng SF, Tseng YH (December 2009). "Genomic characterization of the intron-containing T7-like phage phiL7 of ... Group I introns are also found inserted into genes of a wide variety of bacteriophages of Gram-positive bacteria. However, ...
Abortive initiation
... is typically studied in the T3 and T7 RNA polymerases in bacteriophages and in E. coli. Abortive initiation ... Martin CT, Muller DK, Coleman JE (1988). "Processivity in early stages of transcription by T7 RNA polymerase". Biochemistry. 27 ...
Xenobiology
The GRO exhibited increased resistance to T7 bacteriophage, thus showing that alternative genetic codes do reduce genetic ... Another reason why XB could improve production processes lies in the possibility to reduce the risk of virus or bacteriophage ...
Restriction modification system
Other viruses, such as bacteriophages T3 and T7, encode proteins that inhibit the restriction enzymes.[citation needed] To ... They found that bacteriophage growing within an infected bacterium could be modified, so that upon their release and re- ... This prevents infection by effectively destroying the foreign DNA introduced by an infectious agent (such as a bacteriophage). ... infection of a related bacterium the bacteriophage's growth is restricted (inhibited) (also described by Luria in his ...
Podoviridae
... is a family of bacteriophage in the order Caudovirales often associated with T-7 like phages. There are 130 species ... Nguyen, Doreen; Ely, Bert (June 2018). "A Genome Comparison of T7-like Podoviruses That Infect Caulobacter crescentus". Current ... Criscuolo, Elena; Spadini, Sara; Lamanna, Jacopo; Ferro, Mattia; Burioni, Roberto (2017). "Bacteriophages and Their ...
EXPOSE
... experiment measured the biologically effective ultraviolet dose in the outer space radiation conditions on bacteriophage T7 and ... study of space environment effect on T7 phage, its DNA and of polycristalline uracil. IMBP (Institute of Biomedical Problems), ...
BLESS
BLISS uses T7 bacteriophage-mediated transcription rather than PCR, reducing errors caused by PCR amplification bias that occur ...
Primase
... between different bacteria and viruses where the primase covalently link to helicase in viruses such as the T7 bacteriophage. ... The T7 phage gp4 is a DnaG primase-helicase fusion, and performs both functions in replication. Bocquier AA, Liu L, Cann IK, ...
Ancestral reconstruction
This concept has been validated by an experimental evolutionary study in which replicate populations of bacteriophage T7 were ... "Independent contrasts succeed where ancestor reconstruction fails in a known bacteriophage phylogeny". Evolution; International ...
Tagetitoxin
In contrast, eukaryotic RNA polymerase I and II as well as single-subunit RNA polymerases of bacteriophage T7 and SP6 are ...
Autographiviridae
Since the 1990s, the term "T7 supergroup" has been coined for the expanding group of bacteriophages related to coliphage T7, as ... "Genomic analysis of bacteriophages SP6 and K1-5, an estranged subgroup of the T7 supergroup". Journal of Molecular Biology. 335 ... Enterobacteriaceae phages SP6 and K1-5 were the first to be considered as an estranged subgroup of the "T7 supergroup". ...
Protein moonlighting
Upon infection with the bacteriophage T7, E. coli thioredoxin forms a complex with T7 DNA polymerase, which results in enhanced ... T7 DNA replication, a crucial step for successful T7 infection. Thioredoxin binds to a loop in T7 DNA polymerase to bind more ... The anti-oxidant function of thioredoxin is fully autonomous and fully independent of T7 DNA replication, in which the protein ...
Operon
... s are also found in viruses such as bacteriophages. For example, T7 phages have two operons. The first operon codes for ... "Bacteriophage Use Operons". Prokaryotic Gene Control. Dartmouth College. Archived from the original on 28 January 2013. ... "Displacements of prohead protease genes in the late operons of double-stranded-DNA bacteriophages". Journal of Bacteriology. ... various products, including a special T7 RNA polymerase which can bind to and transcribe the second operon. The second operon ...
Expanded genetic code
Producing better protein: the evolution of T7 bacteriophages on a non-evolving E. coli strain that encoded 3-iodotyrosine on ... Hammerling MJ, Ellefson JW, Boutz DR, Marcotte EM, Ellington AD, Barrick JE (March 2014). "Bacteriophages use an expanded ...
Ellen Jorgensen
"Discrimination between bacteriophage T3 and T7 promoters by the T3 and T7 RNA polymerases depends primarily upon a three base- ... "Specific labelling of the active site of T7 RNA polymerase". Nucleic Acids Research. 15: 8773-81. doi:10.1093/nar/15.21.8773. ...
POLRMT
... it is more closely related to RNA polymerases of bacteriophage (including T7 RNA polymerase), mitochondrial polymerases of ...
Orders of magnitude (length)
... height of a T7 bacteriophage 90 nm - Human immunodeficiency virus (HIV) (generally, viruses range in size from 20 nm to 450 nm ... "Electrospray versus Nebulization for Aerosolization and Filter Testing with Bacteriophage Particles". Aerosol Science and ...
Polynucleotide 5'-hydroxyl-kinase
Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ...
Phage display
The most common bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also ... In T7 phage display, the protein to be displayed is attached to the C-terminus of the gene 10 capsid protein of T7. The ... Many genetic sequences are expressed in a bacteriophage library in the form of fusions with the bacteriophage coat protein, so ... Malys N, Chang DY, Baumann RG, Xie D, Black LW (2002). "A bipartite bacteriophage T4 SOC and HOC randomized peptide display ...
Nuclease protection assay
T7 or T3. These promoters are recognized by DNA dependent RNA polymerases originally characterized from bacteriophages. The ...
Phage-assisted continuous evolution
... the desired protease cut site is used to link a T7 RNA polymerase and a T7 lysozyme. The T7 lysozyme prevents the T7 polymerase ... It relies on relating the desired activity of a target protein with the fitness of an infectious bacteriophage which carries ... The T7 polymerase can only function when the N-terminus portion can bind to the rest of the polymerase. Since APOBEC1 must be ... To evolve APOBEC1 for better soluble expression, the N-terminus of a T7 polymerase was fused to APOBEC1, with the remaining ...
Abraham Eisenstark
Hartman, P. S.; Eisenstark, A.; Pauw, P. G. (1979). "Inactivation of phage T7 by near-ultraviolet radiation plus hydrogen ... Eisenstark, Abraham (2014). "Life in Science: Abraham Eisenstark". Bacteriophage. 4 (3): e29009. doi:10.4161/bact.29009. PMC ... the discovery that bacteriophage can transfer plasmid genes as well as chromosomal genes; and the establishment of the ...
Vector (molecular biology)
Promoter - commonly used inducible promoters are promoters derived from lac operon and the T7 promoter. Other strong promoters ... Brown TA (2010). "Chapter 2 - Vectors for Gene Cloning: Plasmids and Bacteriophages". Gene Cloning and DNA Analysis: An ...
RNA thermometer
This RNA thermometer is now thought to encourage entry to a lytic cycle under heat stress in order for the bacteriophage to ... the gene fusion was then transcribed from the T7 promoter in E. coli, and fluorescence was observed at 37 °C but not at 30 °C. ... Altuvia S, Kornitzer D, Teff D, Oppenheim AB (1989-11-20). "Alternative mRNA structures of the cIII gene of bacteriophage ... Altuvia S, Oppenheim AB (July 1986). "Translational regulatory signals within the coding region of the bacteriophage lambda ...
T7 expression system
Basal levels of expression of T7 RNA polymerase in the cell are also inhibited by the bacteriophage T7 lysozyme, which results ... T7 RNA polymerase is responsible for beginning transcription at the T7 promoter of the transformed vector. The T7 gene is ... The sequencing and annotating of the genome of the T7 bacteriophage took place in the 1980s at the U.S. Department of Energy's ... This polymerase originates from the T7 phage, a bacteriophage virus which infects E. coli bacterial cells and is capable of ...
Lysin
Similarly, not all bacteriophages synthesize lysins: some small single-stranded DNA and RNA phages produce membrane proteins ... T7-like, EC 3.5.1.28) γ-D-glutaminyl-L-lysine endopeptidase (EC 3.4.14.13) Peptidoglycan consists of cross-linked amino acids ... Lysins, also known as endolysins or murein hydrolases, are hydrolytic enzymes produced by bacteriophages in order to cleave the ... Baker JR, Liu C, Dong S, Pritchard DG (October 2006). "Endopeptidase and glycosidase activities of the bacteriophage B30 lysin ...
Pseudomonas virus gh1
... (Bacteriophage gh-1) is a bacteriophage capable of infecting susceptible strains of Pseudomonas putida. ... Evidence for close relationship to the T7 group". Journal of Virology. 311 (2): 305-315. doi:10.1016/S0042-6822(03)00124-7. ... "Pseudomonas putida bacteriophage gh-1 ATCC ® 12633-B1™". - ATCC database entry for gh-1 "Pseudomonad phage gh-1". - Virus-Host ... Lee, L.; Boezi, J. (1966). "Characterization of bacteriophage gh-1 for Pseudomonas putida". Journal of Bacteriology. American ...
Cloning vector
The bacteriophages used for cloning are the λ phage and M13 phage. There is an upper limit on the amount of DNA that can be ... Some commonly used promoters are the T7 and lac promoters. The presence of a promoter is necessary when screening techniques ... Some plasmids contain an M13 bacteriophage origin of replication and may be used to generate single-stranded DNA. These are ... Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains ...
Optical mapping
Amount of T7 exonuclease must be carefully controlled to avoid overly high levels of double-stranded breaks. Step 4: ... "Bacteriophage strain typing by rapid single molecule analysis". Nucleic Acids Research. 43 (18): e117. doi:10.1093/nar/gkv563. ... Step 3: Gap formation T7 exonuclease is added which uses the nicks in the DNA molecules to expand the gaps in a 5'-3' direction ...
Polymerase
Right hand structure of Bacteriophage RB69, a family B DdRP. Central dogma of molecular biology Exonuclease Ligase Nuclease PCR ... T7 RNA polymerase, POLRMT Primase, PrimPol RNA replicase (RNA-directed RNA polymerase, RdRP) Viral (single-subunit) Eukaryotic ...
Staphylococcus aureus
T7 depedent effector proteins EsaD is DNA endonuclease toxin secreted by S. aureus, has been shown to inhibit growth of ... The genes encoding the components of PVL are encoded on a bacteriophage found in community-associated MRSA strains.[citation ... There are also T7 effector proteins that play role a in pathogenesis, for example mutational studies of S. aureus have ... Novel treatments for S. aureus biofilm involving nano silver particles, bacteriophages, and plant-derived antibiotic agents are ...
List of MeSH codes (B04)
... bacteriophage t4 MeSH B04.123.205.891.230 - bacteriophage t7 MeSH B04.123.230.070 - bacteriophage phi 6 MeSH B04.123.370.400 - ... bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700.230 - bacteriophage t7 MeSH B04.123.150.800 ... bacteriophage p22 MeSH B04.280.090.700.100 - bacteriophage t3 MeSH B04.280.090.700.230 - bacteriophage t7 MeSH B04.280.090.800 ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
BP holin family
Wang, I. N.; Smith, D. L.; Young, R. (2000-01-01). "Holins: the protein clocks of bacteriophage infections". Annual Review of ... San Diego found that the BP-Hol family is most closely related to the T7 holin family (TC# 1.E.6). These proteins are of 60 to ... Some are annotated as type II hollins and may be related to members of the T7 Holin family (TC# 1.E.6), although BP-Hol ...
T7 DNA helicase
Helicase Lee SJ, Richardson CC (October 2011). "Choreography of bacteriophage T7 DNA replication". Current Opinion in Chemical ... T7 DNA helicase (gp4) is a hexameric motor protein encoded by T7 phages that uses energy from dTTP hydrolysis to process ... "DNA-induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase". Molecular Cell. 21 (2 ... have proposed a mechanism for the ssDNA-dependent hydrolysis of dTTP by T7 DNA helicase as shown in the figure below. In their ...
DnaG
... bacteriophage, prokaryotic and eukaryotic DNA primases. The primase zinc-binding domain is part of the subfamily of zinc- ... "Interaction of ribonucleoside triphosphates with the gene 4 primase of bacteriphage T7". Journal of Biological Chemistry. 274 ( ...
Bacterial artificial chromosome
T7 & Sp6 phage promoters for transcription of inserted genes. BACs are now being utilized to a greater extent in modelling ... A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. BACs are often used to sequence ...
Spiegelman's Monster
Spiegelman introduced RNA from a simple bacteriophage Qβ (Qβ) into a solution which contained Qβ's RNA replicase, some free ... this time a combination of HIV-1 reverse transcriptase and T7 RNA polymerase. Abiogenesis RNA world hypothesis PAH world ... "Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase ...
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution
Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer- ... Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution Nature. 1998 Jan 15;391(6664):251-8. doi: ... Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer- ...
DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain<...
DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain. Biochemistry. ... DNA recognition by the DNA primase of bacteriophage T7 : a structure-function study of the zinc-binding domain. / Akabayov, ... DNA recognition by the DNA primase of bacteriophage T7 : a structure-function study of the zinc-binding domain. In: ... Dive into the research topics of DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the ...
Synthetic biology - Wikipedia
Chan, Leon Y.; Kosuri, Sriram; Endy, Drew (2005). "Refactoring bacteriophage T7". Molecular Systems Biology. 1: 2005.0018. doi: ... In 2003 the 5386 bp genome of the bacteriophage Phi X 174 was assembled in about two weeks. In 2006, the same team, at the J. ... examples of refactoring including the nitrogen fixation cluster and type III secretion system along with bacteriophages T7 and ... phiX174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences of the United States of ...
Phosphorus in PDB 1a0i: Atp-Dependent Dna Ligase From Bacteriophage T7 Complex with Atp
Atp-Dependent Dna Ligase From Bacteriophage T7 Complex with Atp ... The structure of Atp-Dependent Dna Ligase From Bacteriophage T7 ... H.S.Subramanya, A.J.Doherty, S.R.Ashford, D.B.Wigley. Crystal Structure of An Atp-Dependent Dna Ligase From Bacteriophage T7. ... Phosphorus binding site 1 out of 3 in the Atp-Dependent Dna Ligase From Bacteriophage T7 Complex with Atp. Mono view Stereo ... Phosphorus binding site 2 out of 3 in the Atp-Dependent Dna Ligase From Bacteriophage T7 Complex with Atp. Mono view Stereo ...
Polbase - Reference: Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution.
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution. ... Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution. Doublié S, Tabor S, Long AM, Richardson CC ... Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer- ...
Polbase - Results for Reference: Two forms of the DNA polymerase of bacteriophage T7., Polymerase: T7 (purified without EDTA),...
Two forms of the DNA polymerase of bacteriophage T7., Polymerase: T7 (purified without EDTA), Property: Strand Displacement ... T7 (purified without EDTA) Two forms of the DNA polymerase of bacteriophage T7. Strand Displacement Yes ... Reference: Two forms of the DNA polymerase of bacteriophage T7., Polymerase: T7 (purified without EDTA), Property: Strand ...
Hepatitis B: Practice Essentials, Background, Pathophysiology
A technique for observing extended DNA in negatively stained specimens: observation of bacteriophage T7 capsid-DNA complexes<...
... osmotic shock of bacteriophage T7 and (b) reaction of bacteriophage T7 with glutaraldehyde. Some of the latter DNA-capsid ... osmotic shock of bacteriophage T7 and (b) reaction of bacteriophage T7 with glutaraldehyde. Some of the latter DNA-capsid ... osmotic shock of bacteriophage T7 and (b) reaction of bacteriophage T7 with glutaraldehyde. Some of the latter DNA-capsid ... osmotic shock of bacteriophage T7 and (b) reaction of bacteriophage T7 with glutaraldehyde. Some of the latter DNA-capsid ...
Structure Summary for 1CEZ
CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate: Cell
Molecules | Free Full-Text | Broccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA...
Britton, P.; Green, P.; Kottier, S.; Mawditt, K.L.; Penzes, Z.; Cavanagh, D.; Skinner, M.A. Expression of bacteriophage T7 RNA ... Elroy-Stein, O.; Moss, B. Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in ... T7 RNA polymerase, which can be expressed in mammalian cells [51,52], would provide tight control over transcription regulation ... PCR-amplified DNA templates for complementary splits were added 1:1 as 20% final volume with T7 RNA polymerase (80 mM HEPES-KOH ...
Hepatitis B: Practice Essentials, Background, Pathophysiology
Team:UT-Tokyo/Sudoku reference - 2010.igem.org
T7 RNA polymerase. 1.Characterization of two types of termination signal for bacteriophage T7 RNA polymerase. Macdonald LE, ... 1.Studies on Sex Pili: Mutants of the Sex Factor F in Escherichia coli Defective in Bacteriophage-Adsorbing Function of F Pili ... 2.Crystal structure of an RNA bacteriophage coat protein-operator complex. Karin Valegard, James B. Murray, Peter G. Stockley, ...
mRNA vaccines - a new era in vaccinology | Nature Reviews Drug Discovery
Simone Pieretti | IRB Barcelona
Black box of phage-bacterium interactions: exploring alternative phage infection strategies<...
MeSH Browser
Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued ... Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued ... Bacteriophages [B04.123] * Coliphages [B04.123.205] * Bacteriophage HK022 [B04.123.205.200] * Bacteriophage lambda [B04.123. ... Bacteriophages T Term UI T039960. Date04/16/1979. LexicalTag NON. ThesaurusID UNK (19XX). ...
Measuring protein stability in the GroEL chaperonin cage reveals massive destabilization | eLife
US Patent for Oligonucleotides for modulating protein kinase C having phosphorothioate linkages of high chiral purity Patent ...
Brody, R. et al., "Stereochemical coruse of nucleotidyl catalyzed by bacteriophage T7 induced DNA polymerase", Biochemistry ... employed T7 coliphane DNA having seventeen promoters and one termination site for T7 RNA polymerase. In vitro synthesis by T7 ... For synthesis, a T7 promoter and a template containing the complementary target sequence and T7 promoter hybridization sequence ... Due to this requirement, the T7 RNA polymerase was derived from a strain of E. coli that contained a T7 RNA polymerase ...
The physics of going viral | EurekAlert!
In the case of a bacteriophage called T7, for instance, previous work has shown that the host cell actually grabs onto the DNA ... The researchers used a fluorescent dye to stain the DNA of two mutants of a bacteriophage known as lambda bacteriophage-one ... And so, when the bacteriophages try to inject their DNA into the cells, the factor that limits the rate of transfer is how jam- ... image: A cartoon schematic (top) and raw data (bottom) showing a lambda bacteriophage attached to an ,I,E. coli ,/I,cell with ...
Team:Calgary/Community/Podcasts/Transcripts - 2010.igem.org
The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway | BMC Microbiology | Full...
Tabor S, Richardson CC: A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes ... Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227: 680-685. ... T D, Kuhn A: Hydrophobic forces drive spontaneous membrane insertion of the bacteriophage Pf3 coat protein without topological ... about 109 T7 phages were added, and the mixture was incubated for 30 minutes at 37°C to eliminate remaining E. coli cells. ...
Deciphering the transcriptional changes in Escherichia coli strains C41(DE3) and C43(DE3) that makes them a superior choice for...
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986;189(1):113- ... The BL21(DE3) strain had been engineered to expresses a bacteriophage T7 RNA polymerase (T7RNAP), to transcribe a gene of ... Iost I, Guillerez J, Dreyfus M. Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo. J Bacteriol. 1992;174(2 ... mutations in the lacUV5 promoter region upstream from the bacteriophage T7 RNA polymerase gene distinguish these strains from ...
Transfer and expression of the genes of Bacillus branched chain alpha-oxo acid decarboxylase in Lycopersicun esculentum | Wang...
STUDIER, F.W. and MOFFATT, B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned ... E. coli S17-1 [RP4-2(Tc::Mu)(Km::T7) TpSmProres-mod+ recA-] was used as the donor strain for conjugal transfer of genes to ... l D69lacUV5-T7 gene1) strain was used for over expression of the alpha and beta polypeptides. This strain is a recA derivative ... They were then subcloned to the T7 promoter of the plasmid pBluescript II KS +/-, resulting in the formation of pBluescript II- ...
Find Research outputs - Augusta University Research Profiles
Evolution of regulatory interactions controlling floral asymmetry | Development | The Company of Biologists
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. ... Improved high-level expression system for eukaryotic genes in Escherichia coli using T7 RNA polymerase and rare ArgtRNAs. ... cells were transformed with the construct and the recombinant His-CYC fusion protein was expressed under the control of the T7 ...
DNA-directed RNA polymerase ~ ViralZone
Escherichia phage T7 (Bacteriophage T7) reference strain RPOL_BPT7 T7 RNA polymerase (DNA-directed RNA polymerase) (EC 2.7.7.6) ... Enterobacteria phage T3 (Bacteriophage T3) HELIC_BPT3 DNA helicase/primase (EC 2.7.7.-) (EC 3.6.4.12) (Gene product 4) (Gp4) ... Enterobacteria phage P4 (Bacteriophage P4) reference strain PRIM_BPP4 Putative P4-specific DNA primase (EC 2.7.7.-) (EC 3.6. ... Enterobacteria phage N4 (Bacteriophage N4) reference strain RPOLV_BPN4 Virion DNA-directed RNA polymerase (vRNAP) (EC 2.7.7.6 ...
"NON-FOULING AFFINITY PLATFORMS FOR PROTEIN IMMOBILIZATION IN ELECTRON " by Christopher Jerome Benjamin
PolymerasePhageEscherichiaPhagesBacteriaProteinReplicationVirusesPromoterGenomeStrainsGeneCapsidProteinsColiCharacterizationTranscriptionSingle-subunitSynthesisAbstractInfectsLigaseVitroBacterial infectionsEnzymeRNAPVirulentCRISPR-CasPolymerasesTailPeptideVirionPrimaseMutationNucleotideReactionIsolateSiphoviridaeSystemHostPseudomonasPeptidoglycanTerminatorResistance
Polymerase36
- Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. (nih.gov)
- Gp5 (encoded by gene gp5) is T7 phage's DNA polymerase. (wikipedia.org)
- T7 polymerase uses E. coli's endogenous thioredoxin, a REDOX protein, as a sliding DNA clamp during phage DNA replication (though thioredoxin normally has a different function). (wikipedia.org)
- T7 DNA polymerase, assisted by E. coli thioredoxin, performs both leading and lagging-strand DNA synthesis. (wikipedia.org)
- Results for Reference: Two forms of the DNA polymerase of bacteriophage T7. (neb.com)
- At the genome level, amongst only a handful of genetic changes, mutations in the lac UV5 promoter region upstream from the bacteriophage T7 RNA polymerase gene distinguish these strains from BL21(DE3) but do not inform on how the strains have adapted for superior production of recombinant membrane proteins. (researchsquare.com)
- The BL21(DE3) strain had been engineered to expresses a bacteriophage T7 RNA polymerase (T7RNAP), to transcribe a gene of interest at high efficiency, thus producing large amounts of the corresponding protein (7, 8). (researchsquare.com)
- The reaction is catalyzed by bacteriophage T7 RNA polymerase that incorporates labeled NTPs (mostly UTP) as substitute for their natural counterpart using linear, RNA probe-encoding DNA as template. (jenabioscience.com)
- One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent from infection with a helper virus. (utmb.edu)
- Optimization of a T7-RNA polymerase system in Synechococcus sp. (elsevier.com)
- Dive into the research topics of 'Optimization of a T7-RNA polymerase system in Synechococcus sp. (elsevier.com)
- Initiation by RNA polymerase on UV or x-ray damaged T7 DNA. (wikidata.org)
- T7 promoter.dna ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). (firebaseapp.com)
- Aliquots of DNA from sectors prepared from high titer phage were subjected to in vitro transcription using T7 polymerase and thereafter RNA was translated in a cell-free rabbit reticulocyte system in the presence of [ 35 S] methionine. (elsevier.com)
- Although mitochondria and chloroplasts are considered to be descendants of eubacteria-like endo- symbionts, the mitochondrial RNA polymerase of yeast is a nucleus-encoded, single-subunit enzyme homologous to bacteriophage T3 and T7 RNA polymerases, rather than a multi-component, eubacterial-type alpha 2 beta beta' enzyme, as encoded in chloroplast DNA. (omicsdi.org)
- To broaden our knowledge of the mitochondrial transcriptional apparatus, we have used a polymerase chain reaction (PCR) approach designed to amplify an internal portion of phage T3/T7-like RNA polymerase genes. (omicsdi.org)
- We infer that the T3/T7-like RNA polymerase sequences reported here are likely derived from genes encoding the mitochondrial RNA polymerase in the organisms in which they occur, suggesting a phage T3/T7-like RNA polymerase was recruited to act in transcription in the mitochondrion at an early stage in the evolution of this organelle. (omicsdi.org)
- 1633 /note='T7 promoter' /note='promoter for bacteriophage T7 RNA polymerase' protein_bind 1634. (altiliburda.com)
- 2455 /note='T7 terminator' /note='transcription terminator for bacteriophage T7 RNA polymerase' rep_origin 2548. (altiliburda.com)
- 4131 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(4189. (novoprolabs.com)
- Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. (croyezbio.com)
- 1X RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. (croyezbio.com)
- T7 RNA Polymerase is supplied in 100 mM Tris-HCl (pH 7.9), 20 mM KCl, 1 mM DTT, 1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) glycerol. (croyezbio.com)
- 3. The volume of T7 RNA Polymerase can be titrated between 1-2 μL in the IVT reaction to optimize your assay. (croyezbio.com)
- The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. (kevinkongwellness.com)
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. (kevinkongwellness.com)
- The method requires a controlable and competitive expression system like the bacteriophage T7 polymerase/promoter in a methionine-auxotrophic host. (tu-berlin.de)
- We added new information from the literature to the Main Page of an already existing Part T7 RNA Polymerase ( Part:BBa_I2032 ). (igem.wiki)
- T7 RNA Polymerase (T7 RNAP) is a single subunit protein originating from T7 bacteriophage that catalyzes RNA synthesis. (igem.wiki)
- Compared to the RNA polymerase in E. coli T7 has several advantages. (igem.wiki)
- For RNA production, we use the in vitro transcription approach which utilizes the T7 promoter upstream of a DNA template sequence of interest to facilitate highly efficient production of RNA by the bacteriophage T7 RNA polymerase (RNAP), under appropriate reaction conditions and in the presence of nucleotide triphosphates. (vectorbuilder.com)
- A number of mammalian enzymes have been expressed in Escherichia coli using the T7 RNA polymerase in escherichia coli using the T7 RNA polymerase system, but the production of large amounts of these proteins has been limited by the low percentage of active enzyme that is found in the soluble fraction. (elsevier.com)
- We also tested the effect using a host cell strain that contains a plasmid encoding T7 lysozyme, an inhibitor of T7 RNA polymerase. (elsevier.com)
- They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter. (zymoresearch.com)
- The first operon codes for various products, including a special T7 RNA polymerase which can bind to and transcribe the second operon. (explained.today)
- BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system. (sigmaaldrich.com)
Phage32
- Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. (wikipedia.org)
- Before being physically referred to as T7, the phage was used in prior experiments. (wikipedia.org)
- The genome of phage T7 was among the first completely sequenced genomes and was published in 1983. (wikipedia.org)
- T7 has a life cycle of 17 min at 37˚C, i.e. the time from infection to the lysis of the host cell when new phage are released. (wikipedia.org)
- The T7 phage recognizes certain receptors on the surface of E.coli cells, and binds to the cell surface by its viral tail fibers. (wikipedia.org)
- The short, stubby tail of the T7-like phage is too short to span the cell envelope and, in order to eject the phage genome into the cell at the initiation of infection, virion proteins must first make a channel from the tip of the tail into the cell cytoplasm. (wikipedia.org)
- Once the T7 phage has inserted the viral genome, the process of DNA replication of the host genome is halted and replication of viral genome begins. (wikipedia.org)
- citation needed] Under optimal conditions, the T7 phage can complete the lytic process within 25 minutes, leading to the death of the E. coli host cell. (wikipedia.org)
- Phage T7 has the simplest known DNA replisome, consisting of a helicase and primase that reside in a single polypeptide chain that forms a hexamer in the presence of DNA and ATP or dTTP. (wikipedia.org)
- In phage T7, DNA double-strand breaks are likely repaired by insertion of a patch of donor DNA into a gap at the break site. (wikipedia.org)
- BACTERIOPHAGE T4 ), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. (nih.gov)
- The researchers used a fluorescent dye to stain the DNA of two mutants of a bacteriophage known as lambda bacteriophage-one with a short genome and one with a longer genome-while that DNA was still inside the phage. (eurekalert.org)
- Here, we created a collection of Escherichia coli strains with type I-E CRISPR-Cas system targeting various positions in the genomes of bacteriophages λ, T5, T7, T4 and R1-37 and investigated the ability of these strains to resist the infection and acquire additional CRISPR spacers from the infecting phage. (skoltech.ru)
- Unlike the filamentous systems, peptides or proteins displayed on the surface of T7 do not need to be capable of secretion through the cell membrane, a necessary step in filamentous phage assembly. (emdmillipore.com)
- It is very easy to grow and replicates more rapidly than either bacteriophage l or filamentous phage. (emdmillipore.com)
- The T7 phage particle is extremely robust, and is stable to harsh conditions that inactivate other phage. (emdmillipore.com)
- The T7Select Phage Display System uses the T7 capsid protein to display peptides or proteins on the surface of the phage. (emdmillipore.com)
- This finding provided the initial suggestion that the T7 capsid shell could accommodate variation, and that the region of the capsid protein unique to 10B might be on the surface of the phage and could be used for phage display. (emdmillipore.com)
- Using this strategy, we have recovered sequences homologous to yeast mitochondrial and phage T3/T7 RNA polymerases from a phylogenetically broad range of multicellular and unicellular eukaryotes. (omicsdi.org)
- It is usually thought that bacteriophage T7 is female specific, while phage T3 can propagate on male and female Escherichia coli. (omicsdi.org)
- T7 RNAP was isolated for the first time in 1969 from E. coli that was infected by T7 phage (Tunitskaya & Kochetkov, 2002). (igem.wiki)
- Among them is the bacteriophage virus T5, which is a lytic phage. (pdfkul.com)
- Molineux, I.J. No syringes please, ejection of phage T7 DNA from the virion is enzyme driven. (nature.com)
- In contrast, when E. coli was attacked with T7 phage, S. enterica, the nonhost species, reached higher yields compared with no-phage controls. (umn.edu)
- We carried out plate evolution experiments with individual host colonies and each of 10 phage isolated from each of the bacteriophage strains. (uidaho.edu)
- The bacteriophage strains we are using are listed in Table 1 and include eight ssDNA microviruses and two dsDNA phage, T7 and T3. (uidaho.edu)
- The results of this experiment will hopefully provide further understanding on how to combat bacterial resistance to bacteriophage and, in turn, help increase the effectivity of phage therapy. (uidaho.edu)
- Sillankorva S, Neubauer P, Azeredo J: Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescens. (mirna1.com)
- Experiments using bacteriophage (phage) to infect bacterial strains have helped define some basic genetic concepts in microbiology, but our understanding of the complexity of bacterium-phage interactions is still limited. (pacb.com)
- His lab is now working on a range of topics in synthetic biology, microbiology, and virology including improving phage for therapeutic use against uropathogenic E. coli ,_ _and using next-generation CRISPR methods to engineer T4 and T7 phages. (phageaustralia.org)
- Operons are also found in viruses such as bacteriophage s. [6] [7] For example, T7 phage s have two operons. (explained.today)
- After irreversible adsorbing Necrostatin-1 distributor to cellular receptors, the phage T7 (podovirus) ejects the proteins composing. (aksumuniversity.org)
Escherichia6
- T7 grows on rough strains of Escherichia coli (i.e. those without full-length O-antigen polysaccharide on their surface) and some other enteric bacteria, but close relatives also infect smooth and even capsulated strains. (wikipedia.org)
- From laboratory host Escherichia coli , we have isolated colonies that demonstrated resistance to each of 10 selected types of bacteriophage (Table 1. (uidaho.edu)
- Characterization of a T4-like bacteriophage vB_EcoM-Sa45lw as a potential biocontrol agent for Shiga toxin-producing Escherichia coli O45 contaminated on Mung Bean seeds. (usda.gov)
- Mutants in Escherichia coli transcription termination factor Rho, termed rho(nusD), were previously isolated based on their ability to block the growth of bacteriophage T4. (eurekamag.com)
- Transposon insertion sequencing elucidates novel gene involvement in susceptibility and resistance to phages T4 and T7 in Escherichia coli O157. (pacb.com)
- for example, 84% and 65% of single mutants in bacteriophage T4 lysozyme and the Escherichia coli lac repressor, respectively, were previously shown to be functional [3] , [4] . (plos.org)
Phages6
- E. coli is more resistant to T7 than to some other similar phages. (wikipedia.org)
- In that earlier setup, they had essentially tricked the bacteriophages into ejecting their DNA into solution-a task that the phages completed in less than 10 seconds. (eurekalert.org)
- Lytic bacteriophages (phages) are a diverse family of viruses capable of infecting bacterial cells, often with single species specificity, rapidly generating 10-1000 progeny per infected cell. (lstmed.ac.uk)
- The most studied bacteriophages are those that infect the Gram-positive bacterium Mycobacterium smegmatis mc 2 155, with over 4,800 phages isolated and 690 fully sequenced genomes ( http://www.phagesdb.org ). (biomedcentral.com)
- This group of phages has been isolated and sequenced independently from investigators throughout the world and contains many of the well-characterized, historical phages such as Lambda, Mu, T4 and T7. (biomedcentral.com)
- Lytic bacteriophages isolated and characterized from several MSRA strains play crucial roles in the investigation of the potential use of phages and their products as therapeutic agents against infections caused by biofilm-producing MRSA. (biomedcentral.com)
Bacteria7
- To prove this, the researchers used bacteriophages, which are able to infect bacteria using heads of tightly bundled DNA coated in a protein shell. (eurekalert.org)
- The team found that a fabric made with a dye called rose Bengal as the photosensitizer killed 99.9999% of bacteria added to the fabric within 60 minutes of daylight exposure and inactivated 99.9999% of T7 bacteriophage - a virus thought to be more resistant to ROS than some coronaviruses - within 30 minutes. (scitechdaily.com)
- Single-molecule fluorescence resonance energy transfer analysis of resolving enzymes from bacteriophages (T7 endonuclease I), bacteria (RuvC), fungi (GEN1) and humans (hMus81-Eme1) showed that both types of HJ dynamics still occur after enzyme binding. (elsevier.com)
- Here, we use a bacterial virus, a bacteriophage, to deliver CRISPR to bacteria, which is ironic because bacteria normally use CRISPR to kill viruses," said Rodolphe Barrangou, the Todd R. Klaenhammer Distinguished Professor of Food, Bioprocessing and Nutrition Sciences at NC State and corresponding author of a paper describing the research published today in Proceedings of the National Academy of Sciences . (gcini.org)
- The NC State researchers deployed two different engineered bacteriophages to deliver CRISPR-Cas payloads for targeted editing of E. coli , first in a test tube and then within a synthetic soil environment created to mimic soil - a complex environment that can harbor many types of bacteria. (gcini.org)
- In the context of a society that is confronted with an ever-increasing number of antibiotic-resistant bacteria, we build on the previously made recommendations and specifically address how the Nagoya Protocol might impact the further development of bacteriophage therapy. (pdffox.com)
- This was achieved by fusing the antibody binding domain to surface proteins of bacteriophages (8-10) or bacteria (11). (docksci.com)
Protein7
- Gene 6 protein of bacteriophage T7 is a 5′-3′ exonuclease specific for dsDNA. (divbiolchem.org)
- T7 gene 2.5 single-stranded DNA binding protein stimulates the exonuclease and also the endonuclease activity. (divbiolchem.org)
- Here the major capsid gene of the bacteriophage T7 (40-kb dsDNA) was replaced with the homologous gene of either T3 or K11, each 22% different at the protein level from the T7 homolog. (omicsdi.org)
- Initial fitness was moderately impaired for the T3 exchange, but the K11 exchange was not viable without a compensatory change in the T7 scaffolding protein. (omicsdi.org)
- Berget, P.B. & Poteete, A.R. Structure and functions of the bacteriophage P22 tail protein. (nature.com)
- The tree below was made from a gene H (pilot protein) alignment and does not include T7 or T3. (uidaho.edu)
- In most cases, sRNA-mediated regulation requires the presence of Hfq, a host protein that is required for Qβ bacteriophage replication ( Vogel and Luisi, 2011 ). (molcells.org)
Replication2
- Synthesis of oligoribonucleotide primers for lagging-strand DNA synthesis in the DNA replication system of bacteriophage T7 is catalyzed by the primase domain of the gene 4 helicase-primase. (bgu.ac.il)
- Here we use single-molecule techniques to visualize, in real time, the formation and release of replication loops by individual replisomes of bacteriophage T7 supporting coordinated DNA replication. (blogspot.com)
Viruses3
- Shedding light on the early stages of infection by this type of virus-a bacteriophage-the scientists have determined that it is the cells targeted for infection, rather than the amount of genetic material within the viruses themselves, that dictate how quickly the bacteriophage's DNA is transferred. (eurekalert.org)
- The results also showed that, unlike the viruses that infect humans, bacteriophages transmit only their genetic information into their bacterial targets, leaving their "bodies" behind. (eurekalert.org)
- Bacteriophages, like all viruses, are obligate intracellular parasites that need a host to multiply. (biomedcentral.com)
Promoter1
- It is known for its high processivity and great specificity to the T7 promoter. (igem.wiki)
Genome2
- Recognition and analysis of genome structure and genes function are the required steps before bacteriophages can be approved as therapeutic agents. (hanspub.org)
- Invitrogen사에서 개발한 Gateway cloning법은 bacteriophage λ가 자신의 genome을 E.coli chromosome에 intergration하거나 excision하는 과정에서 작용하는 효소들을 이용하여 site-specific (att) recombination으로 유전자를 cloning하는 방법입니다. (cosmogenetech.com)
Strains3
- However, high-fitness strains of T7 have been isolated with a latent period of only ~11 min at 37˚C growing under optimal conditions in rich media results. (wikipedia.org)
- In some strains of T7, the tail fibers are replaced with tail-spikes that degrade the O- or K-antigens on the cell surface by way of enzymatic activity. (wikipedia.org)
- The objective of this project is to identify mutations in E. Coli C colonies resistant to one of ten different strains of bacteriophage. (uidaho.edu)
Gene2
- The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. (kevinkongwellness.com)
- Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. (zymoresearch.com)
Capsid1
- Serwer, P 1978, ' A technique for observing extended DNA in negatively stained specimens: observation of bacteriophage T7 capsid-DNA complexes ', Journal of Ultrasructure Research , vol. 65, no. 2, pp. 112-118. (uthscsa.edu)
Proteins4
- The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins has been observed. (purdue.edu)
- Analysis of bacteriophage T7 early RNAs and proteins on slab gels. (wikidata.org)
- The library contains all proteins expressed as overlapping 90mer peptide tiles on the surface of bacteriophages. (cdi.bio)
- Israel, V. E proteins of bacteriophage P22. (nature.com)
Coli2
- Both the engineered bacteriophages, called T7 and lambda, successfully found and then delivered payloads to the E. coli host on the lab bench. (gcini.org)
- T7 infection increased nonhost yield by releasing consumable cell debris, and by driving evolution of partially resistant E. coli that secreted more carbon. (umn.edu)
Characterization2
- T: Isolation and characterization of a Lactobacillus plantarum bacteriophage, ΦJL-1, from a cucumber fermentation. (mirna1.com)
- Carey‒Smith GV, Billington C, Cornelius AJ, Hudson JA, Heinemann JA: Isolation and characterization of bacteriophages infecting Salmonella spp. (mirna1.com)
Transcription2
- The T7 RNAP is a highly robust enzyme and transcription reactions using this enzyme can produce large amounts of functional RNA within a few hours. (vectorbuilder.com)
- These results suggest that it is the RNA-binding ability of Rho rather than its transcription termination function that is important for the inhibition of bacteriophage growth and the shorter bulk mRNA lifetime. (eurekamag.com)
Single-subunit1
- In most organisms, the mitochondrial genes are transcribed by RNA polymerases related to the single-subunit RNA polymerases of bacteriophages like T3 and T7. (omicsdi.org)
Synthesis2
- RNA synthesis during bacteriophage SPO1 development: six classes of SPO1 RNA. (wikidata.org)
- Name": "Applications" }, { "Language": "en", "Value": "Use the enzyme to digest DNA impurities during the synthesis and purification of RNA. (roche.com)
Abstract2
- abstract = "Bacteriophage shape the composition and function of microbial communities. (umn.edu)
- Abstract: This perspective paper follows up on earlier communications on bacteriophage therapy that we wrote as a multidisciplinary and intercontinental expert-panel when we first met at a bacteriophage conference hosted by the Eliava Institute in Tbilisi, Georgia in 2015. (pdffox.com)
Infects2
- Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects. (wikipedia.org)
- Bacteriophage P22 infects Salmonella enterica by injecting its genetic material through the cell envelope. (nature.com)
Ligase2
- Crystal Structure of An Atp-Dependent Dna Ligase From Bacteriophage T7. (atomistry.com)
- Sequence and structural analysis of DNA ligases has shown that these enzymes are built around a common catalytic core, which is likely to be similar in three-dimensional structure to that of T7-bacteriophage ligase. (brighton.ac.uk)
Vitro1
- Plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. (deepdyve.com)
Bacterial infections2
- Bacteriophage therapy is considered as an alternative way of controlling bacterial infections and contaminations. (hanspub.org)
- By reviewing a number of recently conducted case studies with bacteriophages involving patients with bacterial infections that could no longer be successfully treated by regular antibiotic therapy, we again stress the urgency and significance of the development of international guidelines and frameworks that might facilitate the legal and effective application of bacteriophage therapy by physicians and the receiving patients. (pdffox.com)
Enzyme1
- Name": "Positioning" }, { "Language": "en", "Value": "No animal-derived material is added in fermentation, purification and final formulation of this enzyme. (roche.com)
RNAP2
Virulent2
- BACTERIOPHAGE T7 ) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. (nih.gov)
- Keel C, Ucurum Z, Michaux the P, Adrian M, Haas D: Deleterious impact of a virulent bacteriophage on survival and biocontrol activity of Pseudomonas fluorescens strain CHA0 in natural soil. (mirna1.com)
CRISPR-Cas1
- CRISPR-Cas systems provide prokaryotes with adaptive defense against bacteriophage infections. (skoltech.ru)
Polymerases1
- Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage. (omicsdi.org)
Tail3
- Bhardwaj, A., Olia, A.S., Walker-Kopp, N. & Cingolani, G. Domain organization and polarity of tail needle GP26 in the portal vertex structure of bacteriophage P22. (nature.com)
- Bacteriophage P22 tail accessory factor GP26 is a long triple-stranded coiled-coil. (nature.com)
- Cingolani, G., Andrews, D. & Casjens, S. Crystallogenesis of bacteriophage P22 tail accessory factor gp26 at acidic and neutral pH. (nature.com)
Peptide1
- Random peptide libraries displayed by bacteriophage T7 and M13 were employed to identify mimotopes from 4 monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei. (who.int)
Virion1
- A T7 virion, seen through cryo-electron tomography. (onelephantsandbacteria.net)
Primase2
- Furthermore, deletion of the T7 gp4 linker region (located between the primase and helicase domains) results in inefficient loading of the ring-shaped hexamer on DNA [ 8 ], which could also apply to Twinkle. (biomedcentral.com)
- Most Twinkle homologues are predicted to possess a primase domain N-terminally of their helicase domain, similar to the T7 gp4 primase/helicase [ 10 ]. (biomedcentral.com)
Mutation1
- They are bacteriophage T1-resistant ( ton A mutation) and also resistant to streptomycin by virtue of rps L mutation. (sigmaaldrich.com)
Nucleotide2
- The length per nucleotide of duplex DNA from bacteriophage πX174 is 0.29 nm. (uthscsa.edu)
- Individual optimization of labeled NTP/NTP ratio can easily be achieved with the single nucleotide format of our HighYield T7 RNA Labeling Kits . (jenabioscience.com)
Reaction1
- The above technique has been used to observe complexes of bacteriophage T7 DNA with T7 capsids produced by (a) osmotic shock of bacteriophage T7 and (b) reaction of bacteriophage T7 with glutaraldehyde. (uthscsa.edu)
Isolate2
- In this project you will Isolate novel bacteriophage and screen them for their diagnostic potential (host range, progeny rate, cycle speed), and characterise them via next generation sequencing. (lstmed.ac.uk)
- Choi, Changsun 2017-02-15 00:00:00 The aim of this study was to isolate and characterize Bacillus cereus bacteriophages of various origins. (deepdyve.com)
Siphoviridae1
- Transmission electron microscopy confirmed B. cereus bacteriophages belonging to the family Siphoviridae. (deepdyve.com)
System1
- T7 bacteriophage has been evolved to override several of the host bacteria's defenses including the peptidoglycan cell wall and the CRISPR system. (wikipedia.org)
Host1
- Among B. cereus bacteriophages with broad host range, 18 isolates (66.7%) did not harbor any B. cereus virulence factors. (deepdyve.com)
Pseudomonas1
- The experiment was realized by Gabriele Martino and Marina Ciuffo ([email protected], [email protected]).Bacteriophages were originally isolated using Pseudomonas syringae pv. (pasteur.cloud)
Peptidoglycan1
- Moak, M. & Molineux, I.J. Peptidoglycan hydrolytic activities associated with bacteriophage virions. (nature.com)
Terminator1
- 4131 /regulatory_class="terminator" /note="T7 terminator" terminator 4085. (novoprolabs.com)
Resistance1
- This resistance remains a barrier to the implementation of bacteriophages as diagnostic tools. (lstmed.ac.uk)