A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Bacteriophage phi X 174
Bacteriophage phi 6
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Viral Tail Proteins
DNA-Directed DNA Polymerase
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
Amino Acid Sequence
Centrifugation, Density Gradient
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Nucleic Acid Conformation
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Genetic Complementation Test
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 18.104.22.168.
RNA Ligase (ATP)
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Site-Specific DNA-Methyltransferase (Adenine-Specific)
An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 22.214.171.124.
Electrophoresis, Polyacrylamide Gel
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Electrophoresis, Agar Gel
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Attachment Sites, Microbiological
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Promoter Regions, Genetic
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme "donor:acceptor group transferase". (Enzyme Nomenclature, 1992) EC 2.
Gene Expression Regulation, Viral
A semisynthetic antibiotic produced from Streptomyces mediterranei. It has a broad antibacterial spectrum, including activity against several forms of Mycobacterium. In susceptible organisms it inhibits DNA-dependent RNA polymerase activity by forming a stable complex with the enzyme. It thus suppresses the initiation of RNA synthesis. Rifampin is bactericidal, and acts on both intracellular and extracellular organisms. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1160)
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Open Reading Frames
Ribonucleoside Diphosphate Reductase
Hydrogen-donating proteins that participates in a variety of biochemical reactions including ribonucleotide reduction and reduction of PEROXIREDOXINS. Thioredoxin is oxidized from a dithiol to a disulfide when acting as a reducing cofactor. The disulfide form is then reduced by NADPH in a reaction catalyzed by THIOREDOXIN REDUCTASE.
Sequence Analysis, DNA
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Sequence Homology, Amino Acid
Deoxyribonuclease (Pyrimidine Dimer)
Sequence Homology, Nucleic Acid
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Drug Resistance, Microbial
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Viral Regulatory and Accessory Proteins
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
Sodium Dodecyl Sulfate
Tungsten hydroxide oxide phosphate. A white or slightly yellowish-green, slightly efflorescent crystal or crystalline powder. It is used as a reagent for alkaloids and many other nitrogen bases, for phenols, albumin, peptone, amino acids, uric acid, urea, blood, and carbohydrates. (From Merck Index, 11th ed)
Rec A Recombinases
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Bacteriophage phiYeO3-12, specific for Yersinia enterocolitica serotype O:3, is related to coliphages T3 and T7. (1/25)Bacteriophage phiYeO3-12 is a lytic phage of Yersinia enterocolitica serotype O:3. The phage receptor is the lipopolysaccharide O chain of this serotype that consists of the rare sugar 6-deoxy-L-altropyranose. A one-step growth curve of phiYeO3-12 revealed eclipse and latent periods of 15 and 25 min, respectively, with a burst size of about 120 PFU per infected cell. In electron microscopy phiYeO3-12 virions showed pentagonal outlines, indicating their icosahedral nature. The phage capsid was shown to be composed of at least 10 structural proteins, of which a protein of 43 kDa was predominant. N-terminal sequences of three structural proteins were determined, two of them showing strong homology to structural proteins of coliphages T3 and T7. The phage genome was found to consist of a double-stranded DNA molecule of 40 kb without cohesive ends. A physical map of the phage DNA was constructed using five restriction enzymes. The phage infection could be effectively neutralized using serum from a rabbit immunized with whole phiYeO3-12 particles. The antiserum also neutralized T3 infection, although not as efficiently as that of phiYeO3-12. phiYeO3-12 was found to share, in addition to the N-terminal sequence homology, several common features with T3, including morphology and nonsubjectibility to F exclusion. The evidence conclusively indicated that phiYeO3-12 is the first close relative of phage T3 to be described. (+info)
Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. (2/25)Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence of thioredoxin. The enhancement in processivity is 20-50-fold when compared with the wild-type Taq DNA polymerase or to the recombinant polymerase in the absence of thioredoxin. The recombinant Taq DNA pol/TBD is thermostable, PCR competent and able to copy repetitive deoxynucleotide sequences six to seven times more faithfully than Taq DNA polymerase and makes 2-3-fold fewer AT-->GC transition mutations. (+info)
The genome sequence of Yersinia pestis bacteriophage phiA1122 reveals an intimate history with the coliphage T3 and T7 genomes. (3/25)The genome sequence of bacteriophage phiA1122 has been determined. phiA1122 grows on almost all isolates of Yersinia pestis and is used by the Centers for Disease Control and Prevention as a diagnostic agent for the causative agent of plague. phiA1122 is very closely related to coliphage T7; the two genomes are colinear, and the genome-wide level of nucleotide identity is about 89%. However, a quarter of the phiA1122 genome, one that includes about half of the morphogenetic and maturation functions, is significantly more closely related to coliphage T3 than to T7. It is proposed that the yersiniophage phiA1122 recombined with a close relative of the Y. enterocolitica phage phiYeO3-12 to yield progeny phages, one of which became the classic T3 coliphage of Demerec and Fano (M. Demerec and U. Fano, Genetics 30:119-136, 1945). (+info)
Characterization of bacteriophage T3 DNA ligase. (4/25)DNA ligases of bacteriophage T4 and T7 have been widely used in molecular biology for decades, but little is known about bacteriophage T3 DNA ligase. Here is the first report on the cloning, expression and biochemical characterization of bacteriophage T3 DNA ligase. The polyhistidine-tagged recombinant T3 DNA ligase was shown to be an ATP-dependent enzyme. The enzymatic activity was not affected by high concentration of monovalent cations up to 1 M, whereas 2 mM ATP could inhibit its activity by 50%. Under optimal conditions (pH 8.0, 0.5 mM ATP, 5 mM DTT, 1 mM Mg(2+) and 300 mM Na(+)), 1 fmol of T3 DNA ligase could achieve 90% ligation of 450 fmol of cohesive dsDNA fragments in 30 min. T3 DNA ligase was shown to be over 5-fold more efficient than T4 DNA ligase for ligation of cohesive DNA fragments, but less active for blunt-ended DNA fragments. Phylogenetic analysis showed that T3 DNA ligase is more closely related to T7 DNA ligase than to T4 DNA ligase. (+info)
In vivo hydrolysis of S-adenosylmethionine induces the met regulon of Escherichia coli. (5/25)Regulation of methionine biosynthesis in Escherichia coli involves a complex of the MetJ aporepressor protein and S-adenosylmethionine (SAM) repressing expression of most genes in the met regulon. To test the role of SAM in the regulation of met genes directly, SAM pools were depleted by the in vivo expression of the cloned plasmid vector-based coliphage T3 SAM hydrolase (SAMase) gene. Cultures with in vivo SAMase activity were assayed for expression of the metA, B, C, E, F, H, J, K and R genes in cells grown in methionine-rich complete media as well as in defined media with and without l-methionine. In vivo SAMase activity dramatically induced expression between 11- and nearly 1000-fold depending on the gene assayed for all but metJ and metH, and these genes were induced over twofold. metJ : : Tn5 (aporepressor defective) and metK : : Tn5 (SAM synthetase impaired; produces <5 % of wild-type SAM) strains containing in vivo SAMase activity produced even higher met gene activity than that seen in comparably prepared cells with wild-type genes for all but metJ in a MetJ-deficient background. The SAMase-mediated hyperinduction of metH in wild-type cells and of the met genes assayed in metJ : : Tn5 and metK : : Tn5 cells provokes questions about how other elements such as the MetR activator protein or factors beyond the met regulon itself might be involved in the regulation of genes responsible for methionine biosynthesis. (+info)
Compensatory evolution in response to a novel RNA polymerase: orthologous replacement of a central network gene. (6/25)A bacteriophage genome was forced to evolve a new system of regulation by replacing its RNA polymerase (RNAP) gene, a central component of the phage developmental pathway, with that of a relative. The experiment used the obligate lytic phage T7 and the RNAP gene of phage T3. T7 RNAP uses 17 phage promoters, which are responsible for all middle and late gene expression, DNA replication, and progeny maturation, but the enzyme has known physical contacts with only 2 other phage proteins. T3 RNAP was supplied in trans by the bacterial host to a T7 genome lacking its own RNAP gene and the phage population was continually propagated on naive bacteria throughout the adaptation. Evolution of the T3 RNAP gene was thereby prevented, and selection was for the evolution of regulatory signals throughout the phage genome. T3 RNAP transcribes from T7 promoters only at low levels, but a single mutation in the promoter confers high expression, providing a ready mechanism for reevolution of gene expression in this system. When selected for rapid growth, fitness of the engineered phage evolved from a low of 5 doublings/h to 33 doublings/h, close to the expected maximum of 37 doublings/h. However, the experiment was terminated before it could be determined accurately that fitness had reached an obvious plateau, and it is not known whether further adaptation could have resulted in complete recovery of fitness. More than 30 mutations were observed in the evolved genome, but changes were found in only 9 of the 16 promoters, and several coding changes occurred in genes with no known contacts with the RNAP. Surprisingly, the T7 genome adapted to T3 RNAP also maintained high fitness when using T7 RNAP, suggesting that the extreme incompatibility of T7 elements with T3 RNAP is not an invariant property of divergence in these expression systems. (+info)
Evolution and the complexity of bacteriophages. (7/25)BACKGROUND: The genomes of both long-genome (> 200 Kb) bacteriophages and long-genome eukaryotic viruses have cellular gene homologs whose selective advantage is not explained. These homologs add genomic and possibly biochemical complexity. Understanding their significance requires a definition of complexity that is more biochemically oriented than past empirically based definitions. HYPOTHESIS: Initially, I propose two biochemistry-oriented definitions of complexity: either decreased randomness or increased encoded information that does not serve immediate needs. Then, I make the assumption that these two definitions are equivalent. This assumption and recent data lead to the following four-part hypothesis that explains the presence of cellular gene homologs in long bacteriophage genomes and also provides a pathway for complexity increases in prokaryotic cells: (1) Prokaryotes underwent evolutionary increases in biochemical complexity after the eukaryote/prokaryote splits. (2) Some of the complexity increases occurred via multi-step, weak selection that was both protected from strong selection and accelerated by embedding evolving cellular genes in the genomes of bacteriophages and, presumably, also archaeal viruses (first tier selection). (3) The mechanisms for retaining cellular genes in viral genomes evolved under additional, longer-term selection that was stronger (second tier selection). (4) The second tier selection was based on increased access by prokaryotic cells to improved biochemical systems. This access was achieved when DNA transfer moved to prokaryotic cells both the more evolved genes and their more competitive and complex biochemical systems. TESTING THE HYPOTHESIS: I propose testing this hypothesis by controlled evolution in microbial communities to (1) determine the effects of deleting individual cellular gene homologs on the growth and evolution of long genome bacteriophages and hosts, (2) find the environmental conditions that select for the presence of cellular gene homologs, (3) determine which, if any, bacteriophage genes were selected for maintaining the homologs and (4) determine the dynamics of homolog evolution. IMPLICATIONS OF THE HYPOTHESIS: This hypothesis is an explanation of evolutionary leaps in general. If accurate, it will assist both understanding and influencing the evolution of microbes and their communities. Analysis of evolutionary complexity increase for at least prokaryotes should include analysis of genomes of long-genome bacteriophages. (+info)
Dispersing biofilms with engineered enzymatic bacteriophage. (8/25)Synthetic biology involves the engineering of biological organisms by using modular and generalizable designs with the ultimate goal of developing useful solutions to real-world problems. One such problem involves bacterial biofilms, which are crucial in the pathogenesis of many clinically important infections and are difficult to eradicate because they exhibit resistance to antimicrobial treatments and removal by host immune systems. To address this issue, we engineered bacteriophage to express a biofilm-degrading enzyme during infection to simultaneously attack the bacterial cells in the biofilm and the biofilm matrix, which is composed of extracellular polymeric substances. We show that the efficacy of biofilm removal by this two-pronged enzymatic bacteriophage strategy is significantly greater than that of nonenzymatic bacteriophage treatment. Our engineered enzymatic phage substantially reduced bacterial biofilm cell counts by approximately 4.5 orders of magnitude ( approximately 99.997% removal), which was about two orders of magnitude better than that of nonenzymatic phage. This work demonstrates the feasibility and benefits of using engineered enzymatic bacteriophage to reduce bacterial biofilms and the applicability of synthetic biology to an important medical and industrial problem. (+info)
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A packaging and delivery system for aerial release of Phytoseiidae for biological control - Semantic Scholar
A new packaging system for the aerial release of phytoseiids was designed and tested in the laboratory and in the field. The observed escapes before and after release, mortality during all stages of release, and fecundity after release indicated that the system was suitable for transporting and distributing phytoseiids. Other evaluated properties of the packaging system included container opening efficiency, container dispersion over the field at several fly-over altitudes, and the probability of containers to be lodged in the cassava canopy. Aerial release trials followed by recoveries of viable adult female phytoseiids from the target fields demonstrated the feasibility of aerial releases as a means for distributing phytoseiid natural enemies.
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Compensatory Evolution in the Human Malaria Parasite Plasmodium ovale | Genetics
THE probability of compensatory genetic change occurring in a gene is very small. The rate of fixation of compensatory mutations in a population depends upon three major factors: (1) effective population size, (2) selection pressure, and (3) mutation rate (Kimura 1990; Stephan 1996). Compensatory evolution has been studied extensively in relation to the RNA structure (Stephan and Kirby 1993; Kirbyet al. 1995; Muse 1995; Stephan 1996; Innan and Stephan 2001). Maintaining the structure of ribosomal RNA (rRNA) is essential to the integrity of the molecule. Mutation in a stem, which leads to a loss of base pairing, will reduce its stability and this is often accompanied by changes in the biochemical properties of the molecule. A second mutation in the complementary region of the RNA that restores the structural integrity most often restores the biochemical properties of the molecule despite changes in the primary sequence (Figure 1A). In essence, the probability of both members of a stem pair ...
Evolution of genetic architectures | Proceedings of the Royal Society of London B: Biological Sciences
The predictions of our model-notably, that the number of loci in a genetic architecture and the variance of their allelic contributions are greatest for traits under intermediate selection-are robust to choices of population-genetic parameters. The non-monotonic relation between selection pressure on a trait and the size of its genetic architecture, L, holds regardless of population size, but the location of maximum L is shifted towards weaker selection in larger populations (see electronic supplementary material, figure S5). This result is compatible with our explanation involving compensatory evolution: selection is more efficient in large populations, and so compensatory evolution occurs at smaller selection coefficients. Likewise, when the mutation rate is smaller the resulting equilibrium number of controlling loci is reduced (see electronic supplementary material, figure S6). This result is again compatible with the explanation of compensatory evolution, which requires frequent mutations. ...
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bin/bash # Package a root filesystem directory into a filesystem image, with # associated bootable kernel binary and launch scripts. source sources/include.sh ,, exit 1 # Parse the sources/targets/$1 directory read_arch_dir $1 # Do we have our prerequisites? if [ -z $NATIVE_ROOT ] then [ -z $NO_NATIVE_COMPILER ] && NATIVE_ROOT=$BUILD/root-filesystem-$ARCH [ -e $NATIVE_ROOT ] ,, NATIVE_ROOT=$BUILD/simple-root-filesystem-$ARCH fi if [ ! -d $NATIVE_ROOT ] then [ -z $FAIL_QUIET ] && echo No $NATIVE_ROOT ,&2 exit 1 fi # Announce start of stage. (Down here after the recursive call above so # it doesnt get announced twice.) echo === Packaging system image from root-filesystem # The initramfs packaging uses the kernels build infrastructure, so extract # it now. setupfor linux [ -z $SYSIMAGE_TYPE ] && SYSIMAGE_TYPE=squashfs echo Generating $SYSIMAGE_TYPE root filesystem from $NATIVE_ROOT. # Embed an initramfs image in the kernel? if [ $SYSIMAGE_TYPE == initramfs ] then $CC ...
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Pelican 1560 Case with Foam | Caseman
In business since 1993, we have developed unsurpassed knowledge about transit cases and foam packaging systems. We are serious about our work and proud of our expertise and product quality.. ...
Integrase-deficient lentivirus (IDLV) packaging system for transient expression in dividing cells and stable expression in quiescent cells.
Integrase-deficient lentivirus (IDLV) packaging system for transient expression in dividing cells and stable expression in quiescent cells.
tech-pkg: Re: Two package-suggestions
While playing around with the packaging system, I kept running into , one problem: losing my dynamic libraries. My guess: set up an /etc/ld.so.conf listing the directories where you have your other shared libraries and I think this problem will disappear. Because you *do* run a new enough system that ldconfig reads this file by itself, dont you? - H=E5vard ...
Lentiviral, Retroviral, Baculoviral Expression Vectors and Particles: Viral packaging systems for a new age
Viral packaging systems in lentiviral, baculoviral, and retroviral platforms as well as packaging services with high titer products
pASSEMBLE™ 10A1 Retroviral Packaging System | Canvax Biotech
pASSEMBLE™ 10A1 Retroviral Packaging System includes a unique packaging vector with gag, pol and env from different viruses confering a tropism in the cell to be infected.
Constantia releases portable blister packaging system
Constantia Flexibles released its new packaging system, the Flexible Blister, which can be incorporated into existing production while offering a portable system for packaging.
Taq DNA Polymerase Guidelines for PCR Optimization | NEB
Introduction Taq DNA Polymerase is an enzyme widely used in PCR (1). The following guidelines are provided to ensure successful PCR using NEBs Taq DNA Polymerase
Taq DNA Polymerase from Bioline
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PCR Using Hot Start Taq DNA Polymerase (M0495) | NEB
PCR The following guidelines are provided to ensure successful PCR using New England Biolabs’ Hot Start Taq DNA Polymerase
Gentaur Molecular :SibEm \ Taq DNA Polymerase with AS Buffer \ E338
Gentaur molecular products has all kinds of products like :search , SibEm \ Taq DNA Polymerase with AS Buffer \ E338 for more molecular products just contact us
Patent US7573547 - System and method for protecting micro-structure of display array using ... - Google Patents
Physical forces sufficient to deform an electronic device and/or packaging for the electronic device can damage the device. Some mechanical components in a device, for example, in a microelectromechanical device and/or in an interferometric modulator are particularly susceptible to damage. Accordingly, provided herein is a packaging system and packaged electronic device that resists physical damage, a method for manufacturing the same, and a method for protecting an electronic device from physical damage. The packaging system for the electronic device includes one or more spacers that prevent or reduce damage to the electronic device arising from contact with the packaging. In some embodiments, the packaged electronic device comprising spacers is thinner than a comparable device manufactured without spacers.
blister sealer machine - Steemwiki.info
blister packing, turn table pvc blister sealing machine blister sealer, starview packaging machinery blister packaging heat, starview blister sealers professional packaging systems, starview blister sealers professional packaging systems
Kevin Cameron joins EDL Packaging Engineers as operations manager | Packaging World
EDL Packaging Engineers, Inc. (EDL) , provider of end-of-line packaging systems, has announced that Kevin Cameron hasjoined the company as operations manager, and will be responsible for optimizing EDLs manufacturing and assembly operations.
titripac-award | China-Mainland | Sigma-Aldrich
The Titripac® packaging system for volumetric solutions is constructed from a recyclable outer carton and a durable inner bag. The inner bag collapses during withdrawal of reagent through the built-in contamination-proof dispenser tap. Therefore the reagent in the bag cannot be contaminated, and when empty, the outer carton can be fully recycled. The amount of packaging per liter is less than half the weight of alternative packaging options (plastic bottles ...
Pre-Opened Poly Bags on a Roll, Automatic Fill Style, 1.5 Mil, 3 x 3, Clear, 4500/roll
Our 1.5 Mil Clear Pre-Opened Bags on Roll are designed for use with auto-baggers, or automated bagging machines, for easy and efficient packaging.. Bags for automatic machines come open on one side, for easy product inserting, and perforated on the back for easy tear-off. Ideal for high-volume packaging operations. Compatible with most automated bagging machines like Titan, Clamcom AUTOBAG, Sharp, and others. Rest assured, these are compatible with most industry standard packaging systems and are very competitively priced. All of our Pre-Opened Bags on Roll meet FDA and USDA requirements.. As with any of our bags, custom printing and sizes are always available.. ...
Machines Vertical Form Fill Seal Machine Vertical Forming Filling Sealing Machines - Packing Machine Manufacturer
Vertical Form Fill & Seal Machines - APPE - Vertical Form, Fill & SealThe Vertical form fill sealing machine (FFS) is a type of automated assembly-line product packaging system, commonly used to in the packaging industry for food, and a wide variety of other products Content on this page requires a newer version ... .Vertical […]
College of Architecture, Arts and Humanities | Case Studies
Working with information and product developers and software engineers at AT&T GIS, the faculty and staff have helped AT&T GIS to cut their support costs and improve their product development cycles. Since 1993, we have worked with AT&T GIS to dramatically improve their software interfaces, user manuals, packaging systems, and mass marketing materials.. Challenges. Because our usability testing research and client training programs have focused on mission-critical products for our industry partners, the usability publications of our faculty and graduate students are frequently proprietary and subject to strict non-disclosure contracts.. Other Clients. ...
Ubuntu* Core is a version of the Ubuntu Linux* distribution modified for use on IoT devices. It employs a snap packaging system, originally developed for Ubuntu Mobile Devices, and brings many phone features to the IoT developer. ...
Cosmetics & Chemical
From cleaning products to skincare, perfumes to make up to bath and shower products - Adpak has packaging systems that can wrap almost anything.
Magnum Systems Appoints Mountain Pacific Machinery as Technical Indust | powderbulksolids.com
Magnum Systems, one of North Americas leading designers and manufacturers of material handling and packaging systems for dry bulk solids, announces an ag
FOF pump-diaphragm - Inline Filling Systems
Inline Filling Systems specializes in manufacturing liquid packaging systems that are engineered and built to meet the industry specific needs of small and medium size companies. We provide high value, robust systems that include a range of container flexibility, are easy to use, easy to clean and easy to maintain for the lowest total cost of ownership.. ...
Food & Beverage
From pastries to sauces, tea bags to tinned goods and milk to soft drinks - Adpak offers shrink wrapping and packaging systems to wrap almost anything.
Ubuntu* Core is a version of the Ubuntu Linux* distribution modified for use on IoT devices. It employs a snap packaging system, originally developed for Ubuntu Mobile Devices, and brings many phone features to the IoT developer. ...
The co-operatives board of directors had already taken special care to design and build halls that were ideal for storage. The new storage areas allow the large crates of fruit to be stacked and stored as ergonomically as possible. Their headroom of more than 10 meters means 13 large crates can be stacked right up to the roof. Growing customer requirements also meant further investments in the very latest sorting and packaging systems. One of Lanas main aims is to meet all the many varied requirements of the market with innovative and proactive solutions ...
US7967138B2 - Packaging systems for percutaneously deliverable bioprosthetic valves - Google Patents
A packaging system is disclosed for shipping a prosthetic tissue valve in a storage solution and preparing and loading of the bioprosthetic valve onto a catheter-based delivery system. The packaging system includes a fluid tight container filled with the storage solution attached to a delivery catheter, wherein the container surrounds the prosthetic tissue valve that is in a pre-loaded position on the delivery catheter during shipment and storage. The prosthetic tissue valve may include an attachment mechanism that attaches to the delivery catheter to properly position the tissue valve for loading within the delivery catheter. In another embodiment where the prosthetic tissue valve is not attached to the delivery catheter during shipment, the attachment mechanism may interact with the prosthetic tissue valve shipping container to prevent the bioprosthetic valve from moving during shipment.
Taq DNA Polymerase
Taq DNA Polymerase is a thermostable DNA Polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA Polymerase is the most common polymerase used for PCR reactions.
MTP Taq DNA Polymerase | China-Mainland | Sigma-Aldrich
MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize the levels of contaminating DNA.
Taq DNA Polymerase - (D0010) - Products - Abnova
Taq DNA polymerase catalyzes 5-3 synthesis of DNA. The enzyme has been proved not having the 3-5 exonuclease activity. The enzyme has proven to have high amplification yield, be stable at high temparetaure. (D0010) - Products - Abnova
Cambridge Bioscience: AAV Helper Free Packaging Systems
Most of the adenovirus gene products required for the production of infective AAV particles are supplied on the pHelper plasmid (i.e. E2A, E4, and VA RNA genes) that is co-transfected into cells with human AAV vector DNA. The remaining adenoviral gene product is supplied by the 293 host cells, which stably expresses the adenovirus E1 gene. By eliminating the need for live helper virus, the AAV helper-free system provides a safer and more convenient gene delivery system ...
User:Dstoebel - OpenWetWare
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution In Press. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. 22:683-690. Full ...
User:Dstoebel - OpenWetWare
D. M. Stoebel, and C. J. Dorman. 2010. The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli. Molecular Biology and Evolution, 27:2105-2112. Full Text D. M. Stoebel, and D. E. Dykhuizen. 2010. Waste and yet want not. Molecular Cell, 38:625-626. Full Text D. M. Stoebel, K. Hokamp, M. S. Last, and C. J. Dorman. 2009. Compensatory evolution of gene regulation in response to stress by E. coli lacking RpoS. PLoS Genetics, 5: e1000671 Full Text D. M. Stoebel, A. Free, and C. J. Dorman. 2008. Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria. Microbiology, 154: 2533-2545. Full Text D. M. Stoebel, A. M. Dean, and D. E. Dykhuizen. 2008. The cost of expression of Escherichia coli lac operon proteins is in the process, not in the products. Genetics, 178:1653-1660. Full Text D. M. Stoebel. 2005. Lack of evidence for horizontal transfer of the lac operon into Escherichia coli. Molecular Biology and Evolution. ...
Environmental epistasis in catalytic RNA | Proceedings of the Royal Society of London B: Biological Sciences
Two trajectories are possible in converting a GC base pair to an AU base pair. One of them passes through a GU base pair intermediate, the other through an AC mismatch intermediate (see figure 4; electronic supplementary material, figure S3). In our data, a ribozyme with the GU intermediate is always more active than that with the AC intermediate (from +1.4 to +22.7%; average = +10.3%). This confirms predictions of the relative effects of these two intermediates based on the geometry of base pairs , experimental measurements  and bioinformatic analysis of tRNA evolution . Our data support a continuous ridge or neutral network model (see Landscapes caused by a base pair switch in Supporting Information, electronic supplementary material) of compensatory evolution in RNA secondary structure, because in our experiments, the GU intermediate shows a significant decrease in fitness only in a single instance, and in this instance the base pair switch is not compensatory but ...
Packaging (disambiguation) - Wikipedia
Active packaging, packaging systems to help extend shelf life, monitor freshness, display information on quality, improve safety, and improve convenience**Self-heating food packaging, active packaging with the ability to heat food contents without external heat sources or ...
പോളിമെറേയ്സ് ചെയിൻ റിയാക്ഷൻ - വിക്കിപീഡിയ
മിശ്രിതത്തെ 55 ഡിഗ്രി സെന്റീഗ്രേഡിലേയ്ക്ക് പതിയെ ഊഷ്മനില താഴ്ത്തുന്നു. ഇവയ്ക്ക് അനുപൂരകമായ അൻപതിൽത്താഴെ ന്യൂക്ലിയോടൈഡുകളുള്ള പ്രൈമറുകൾ നിർമ്മിക്കുന്നു. നാലുതരം ഡിഓക്സിട്രൈന്യൂക്ലിയോടൈഡുകളെ ടാക് (taq) പോളിമെറേയ്സ് രാസാഗ്നിയോടൊപ്പം വേർപെട്ട ഡി.എൻ.ഏ തന്മാത്രകളോടൊപ്പം ചേർക്കുന്നു. താപനില ഉയർത്തി 72 ഡിഗ്രി സെൽഷ്യസാക്കുന്നു. 90 ഡിഗ്രിയിലും നശിച്ചുപോകാത്ത Taq DNA Polymerase എന്ന ...
Apache Hadoop 3.1.4 - Hadoop Cluster Setup
Installing a Hadoop cluster typically involves unpacking the software on all the machines in the cluster or installing it via a packaging system as appropriate for your operating system. It is important to divide up the hardware into functions.. Typically one machine in the cluster is designated as the NameNode and another machine as the ResourceManager, exclusively. These are the masters. Other services (such as Web App Proxy Server and MapReduce Job History server) are usually run either on dedicated hardware or on shared infrastructure, depending upon the load.. The rest of the machines in the cluster act as both DataNode and NodeManager. These are the workers. ...
Protocols and Video Articles Authored by Jinhe Bai
Jinhe Bai is the author of this article in the Journal of Visualized Experiments: Controlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes
Fruit Quality as influenced by Wax Application
DAnjou pears harvested at commercial maturity from a block of 6 trees were immediately divided into 2 lots. One lot was placed in refrigerated storage (1 °C) for 90 days and then transferred to room temperature 24 hours prior to wax application. The second lot of fruit was waxed 24 hours after harvest, using both hot and cold wax drying techniques. Prior to waxing, the pears were drenched in hot water (40 °C), and passed over rotary dewatering brushes for 30 sec before going into the wax applicator. Using a commercial pear wax formulation (Shieldbrite PR160C, Shield-brite Corp, Kirkland. WA) pears were waxed at the approximate rate of 10 ml/min (6,804 Kg fruit/3.8 liters). Waxed pears were dried in a commercial hot-drying tunnel held at 60 °C (Van Doren Sales, Inc., Wenatchee, WA) or a cold drier operated at 0 °C (Sirron, marketed by MARQ International Packaging Systems, Inc., Yakima, WA) for 2 min. After the fruit surface had dried, 100 pears were packed in pulp fiber trays, enclosed in a ...
Protocols and Video Articles Authored by Jan Narciso
Jan Narciso is the author of this article in the Journal of Visualized Experiments: Controlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes
The many length scales of DNA packaging | Essays in Biochemistry | Portland Press
This collection of reviews focuses on the most exciting areas of DNA packaging at the current time. Many of the new discoveries are driven by the development of molecular or imaging techniques, and these are providing insights into the complex world of chromatin. As these new techniques continue to improve, we will be able to answer many of the questions we have now, while likely raising many new ones. ...
... is typically studied in the T3 and T7 RNA polymerases in bacteriophages and in E. coli. Abortive initiation ...
Restriction modification system
Other viruses, such as bacteriophages T3 and T7, encode proteins that inhibit the restriction enzymes. To counteract these ... They found that bacteriophage growing within an infected bacterium could be modified, so that upon their release and re- ... This prevents infection by effectively destroying the foreign DNA introduced by an infectious agent (such as a bacteriophage). ... infection of a related bacterium the bacteriophage's growth is restricted (inhibited) (also described by Luria in his ...
Escherichia virus T4
His part was naming the bacteriophages into Type 1(T1), Type 2 (T2), Type 3 (T3), etc. The specific time and place of T4 virus ... T4-like viruses Animation of T4 Bacteriophage Infecting E.coli Animation of T4 Bacteriophage DNA packaging. ... "Genetic Recombinations Leading to Production of Active Bacteriophage from Ultraviolet Inactivated Bacteriophage Particles". ... Molecular Biology of Bacteriophage T4. ASM Press, Washington, DC. (The second T4 bible, go here, as well as Mosig and Eiserling ...
Genetically modified tomato
Agritope introduced an S-adenosylmethionine hydrolase (SAMase) encoding gene derived from the E. coli bacteriophage T3, which ...
Nuclease protection assay
T7 or T3. These promoters are recognized by DNA dependent RNA polymerases originally characterized from bacteriophages. The ...
"Discrimination between bacteriophage T3 and T7 promoters by the T3 and T7 RNA polymerases depends primarily upon a three base- ...
List of MeSH codes (B04)
... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ... bacteriophage n4 MeSH B04.280.090.700.070 - bacteriophage p22 MeSH B04.280.090.700.100 - bacteriophage t3 MeSH B04.280.090.700. ... bacteriophage t3 MeSH B04.123.205.891.200 - bacteriophage t4 MeSH B04.123.205.891.230 - bacteriophage t7 MeSH B04.123.230.070 ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends: 5'-A ,A G C T T-3' 3'-T T ... Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is ...
Escherichia virus T3
... , also called bacteriophage T3 and T3 phage, is a bacteriophage capable of infecting susceptible bacterial ... FRASER, D; WILLIAMS, RC (Feb 1953). "Details of frozen-dried T3 and T7 bacteriophages as shown by electron microscopy". Journal ... "DNA packaging-associated hyper-capsid expansion of bacteriophage t3". Journal of Molecular Biology. 397 (2): 361-74. doi: ... T3+Phage at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
T7 phage, T3 phage, Φ29, P22, P37 Ligamenvirales Lipothrixviridae. Enveloped, rod-shaped. Linear dsDNA. Acidianus filamentous ... Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found ... A bacteriophage (/bækˈtɪərioʊfeɪdʒ/), also known informally as a phage (/feɪdʒ/), is a virus that infects and replicates within ... 2×108 bacteriophages per mL. Bacteriophages are thought to extensively contribute to horizontal gene transfer in natural ...
T7 RNA polymerase
... swapping it out for one found in T3 RNAP makes the polymerase recognize T3 promoters instead. Similar to other viral nucleic ... T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' ... Other members include phage T3 and SP6 RNA polymerases, the mitochondrial RNA polymerase (POLRMT), and the chloroplastic ssRNAP ... McAllister WT (1993). "Structure and function of the bacteriophage T7 RNA polymerase (or, the virtues of simplicity)". Cellular ...
Genetically modified tomato
... coli bacteriophage T3, which reduced the levels of S-adenosylmethionine, a precursor to ACC. Endless Summer was briefly ...
The DNA-binding protein complex recognizes a discrete nucleotide sequence (5'-GGG ACT TTC T-3') in the upstream region of a ... These microorganisms include bacteria, fungi, viruses and bacteriophages. The bacterial part of the microbiota has been more ...
T7 RNA polymerase
... is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' ... Related family members include phage T3 and SP6 RNA polymerases, but this family is also related to the mitochondrial RNA ... McAllister WT (1993). "Structure and function of the bacteriophage T7 RNA polymerase (or, the virtues of simplicity)". Cell. ... T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with ...
Phage-assisted continuous evolution
It relies on relating the desired activity of a target protein with the fitness of an infectious bacteriophage which carries ... This resulted in polymerases with ~3-4 orders of magnitude greater activity for the target promoter than the original T3 ... The central component of PACE is a fixed-volume vessel known as the "lagoon". The lagoon contains M13 bacteriophage vectors ... Brödel, A.K.; Isalan, M.; Jaramillo, A. (2018). "Engineering of biomolecules by bacteriophage directed evolution". Curr. Opin. ...
Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant (Journal Article) | SciTech Connect
Journal Article: Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant ... Title: Length quantization of DNA partially expelled from heads of a bacteriophage T3 mutant ... 60 APPLIED LIFE SCIENCES; BACTERIOPHAGES; CONTROL; DNA; DNA SEQUENCING; ELECTRON MICROSCOPY; ELECTROPHORESIS; EVOLUTION; GELS; ... We use directed evolution to isolate a five-site T3 point mutant that hyper-produces tail-free capsids with mature DNA (heads ...
The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3,...
The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3, ... Sequence and analysis of the gene for bacteriophage T3 RNA polymerase. Nucleic Acids Res 13:6753-6766 ... Dietz, A., Weisser, H., Kössel, H. et al. The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison ... Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA ...
Identification and characterization of T3/T7 bacteriophage-like RNA polymerase sequences in wheat
... that encode T3/T7 bacteriophage-like RNA polymerases (RNAPs) sharing 45% amino acid identity. In phylogenetic analyses using ... Identification and characterization of T3/T7 bacteriophage-like RNA polymerase sequences in wheat Plant Mol Biol. 1999 Jul;40(4 ... Using PCR-based methods, we assembled two wheat cDNA sequences, wheat-G and wheat-C, that encode T3/T7 bacteriophage-like RNA ...
Mobilization and Reassembly of Genetic Information - 1st Edition
A Restriction Map of Bacteriophage T3. Characterization of T7 Promoter Sequences. The Structure of Transposable Mating Type ... Site-Specific Recombination in Bacteriophage λ: Requirements for the Phage att Site and Organization of the Recombination ... Formation of PlargF Specialized Transducing Bacteriophage: Involvement of IS1. New Synthetic Oligonucleotides for Specific ...
Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA<...
Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA. / Basu, S ... title = "Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA", ... T1 - Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA ... Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA. ...
RCSB PDB - Protein Feature View - Thioredoxin-1 - P0AA25 (THIO ECOLI)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Selection on noise constrains variation in a eukaryotic promoter. - PubMed - NCBI
PGLYRP1 peptidoglycan recognition protein 1 [Homo sapiens (human)] - Gene - NCBI
17.5 - Holin - Enterobacteria phage T3 - 17.5 gene & protein
Enterobacteria phage T7 (Bacteriophage T7). Enterobacteria phage T3 (Bacteriophage T3). Yersinia phage YpP-Y. Yersinia phage R ... Enterobacteria phage T7 (Bacteriophage T7). Enterobacteria phage T3 (Bacteriophage T3). Yersinia phage YpP-Y. Yersinia phage R ... "Mutation in bacteriophage T3 affecting host cell lysis.". Miyazaki J.I., Ryo Y., Fujisawa H., Minagawa T.. Virology 89:327-329( ... Enterobacteria phage T3 (Bacteriophage T3). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ...
Influence of S-Adenosylmethionine Pool Size on Spontaneous Mutation, Dam Methylation, and Cell Growth ofEscherichia coli |...
By regulating the expression of the rat liver SAM synthetase and the bacteriophage T3 SAM hydrolase proteins in E. coli, a 100- ... X. Bacteriophage T3-induced S-adenosylmethionine cleavage. J. Biol. Chem. 241:1995-2006. ... 1994) A novel Tn10 tetracycline regulon system controlling expression of the bacteriophage T3 gene encoding S-adenosyl-L- ... and bacteriophage T3 SAM hydrolase (T3SH). RLSS is a highly conserved homolog of the bacterial MetK SAM synthetase, except that ...
Helicase promotes replication re-initiation from an RNA transcript | Nature Communications
Here the authors find that bacteriophage T7 helicase is also involved in replication re-initiation by interacting with a non- ... Kruger, D. H. & Schroeder, C. Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol. Rev. 45, 9-51 ( ... Here, we investigate this replication re-initiation pathway using the bacteriophage T7 replisome. The bacteriophage T7 ... Mechanisms for the initiation of bacteriophage T7 DNA replication. Cold Spring Harb. Symp. Quant. Biol. 47, 669-679 (1983). ...
Enterobacteria phage T3 (Bacteriophage T3). OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; OC ... "Sequence of a conditionally essential region of bacteriophage T3, RT including the primary origin of DNA replication."; RL J. ... DR InterPro; IPR016306; DNA_ligase_ATP-dep_T3. DR InterPro; IPR012340; NA-bd_OB-fold. DR Pfam; PF01068; DNA_ligase_A_M; 1. DR ... PIRSF; PIRSF001600; DNA_ligase_phage_T3; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR PROSITE; PS00697; DNA_LIGASE_A1; 1. DR PROSITE ...
The T7 Group | SpringerLink
Locations and nucleotide sequences of three major class III promoters for bacteriophage T3 RNA polymerase on T3 DNA, J. Biol. ... Hamada, K., Fujisawa, H., and Minagawa, T., 1986, A defined in vitro system for packaging of bacteriophage T3 DNA, Virology 151 ... Beier, H., and Hausmann, R., 1973, Genetic map of bacteriophage T3, I. Virol. 12: 417.Google Scholar ... Hausmann, R., and Härle, E., 1971, Expression of the genomes of the related bacteriophages T3 and T7, in: Proceedings of the ...
Escherichia virus T3 - Wikipedia
Escherichia virus T3, also called bacteriophage T3 and T3 phage, is a bacteriophage capable of infecting susceptible bacterial ... FRASER, D; WILLIAMS, RC (Feb 1953). "Details of frozen-dried T3 and T7 bacteriophages as shown by electron microscopy". Journal ... "DNA packaging-associated hyper-capsid expansion of bacteriophage t3". Journal of Molecular Biology. 397 (2): 361-74. doi: ... T3+Phage at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
Plastid RNA Polymerases | SpringerLink
Identification and characterization of T3/T7 bacteriophage-like RNA polymerase sequences in wheat. Plant. Mol. Biol. 40, 567- ... Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the ... Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme. J. Mol. Biol. 269, 10-27.CrossRefPubMedGoogle ... RNA polymerase of bacteriophage T7. Mol. Biol. 33, 353-367.Google Scholar ...
T3 DNA Ligase | NEB
T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a ... Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ... T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond ... T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond ...
Patent US6506559 - Genetic inhibition by double-stranded RNA - Google Patents
The RNA may be synthesized by a cellular RNA polymerase or a bacteriophage RNA polymerase (e.g., T3, T7, SP6). The use and ... RNA was synthesized from phagemid clones with T3 and T7 RNA polymerase6, followed by template removal with two sequential DNase ... A general strategy for production of dsRNA is to clone segments of interest between flanking copies of the bacteriophage T7 ... T3 or T7 RNA polymerase), or with characterized promoters in vivo, is produced predominantly from a single strand. The ...
Frontiers | Recent advances in the study of chloroplast gene expression and its evolution | Plant Science
Patent US5466586 - Method for the synthesis of ribonucleic acid (RNA) - Google Patents
Patent US7101697 - Restriction endonucleases, method of synthesis and use thereof - Google Patents
shows clearly that the genomic DNA of bacteriophage T3, which cannot be cleaved by wild-type EcoRII because of the lower ... Cleavage of the DNA of bacteriophage T3 with EcoRII-trunc (here: EcoRII173-404) and wild-type EcoRII. Top: quantities of ... enzymes used; left trace: T3 DNA without restriction endonuclease; BstNI: positive control of the cleavage pattern; right trace ...
Mammalian Mitochondrial DNA (mtDNA) Transcription
Nascent RNA structure modulates the transcriptional dynamics of RNA polymerases | PNAS
1987) Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7. Cell 51:89-99. ... 2006) Bacteriophage origins of mitochondrial replication and transcription proteins. Trends Genet 22:90-95. ... 1998) Pausing and termination by bacteriophage T7 RNA polymerase. J Mol Biol 280:201-213. ... 2004) Promoter binding, initiation, and elongation by bacteriophage T7 RNA polymerase. A single-molecule view of the ...
"ORI bacteriophage f1" FT promoter 0..0 FT /note="PRO E. coli lac gene" FT promoter 0..0 FT /note="PRO bacteriophage T3" FT ... CC The order of the major features in this plasmid is: TRP1 - f1 ori CC (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - pMB1 ... promoter 0..0 FT /note="PRO bacteriophage T7" FT CDS 0..0 FT /note="GEN E. coli beta-galactosidase gene (lacZ); FT reporter ...
Cra-Dependent Transcriptional Activation of theicd Gene of Escherichia coli | Journal of Bacteriology
Outer Membrane Proteins Ail and OmpF of Yersinia pestis Are Involved in the Adsorption of T7-Related Bacteriophage Yep-phi |...
Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol. Rev. 45:9-51. ... Bacteriophage phiYeO3-12, specific for Yersinia enterocolitica serotype O:3, is related to coliphages T3 and T7. J. Bacteriol. ... The genome sequence of Yersinia pestis bacteriophage phiA1122 reveals an intimate history with the coliphage T3 and T7 genomes ... Antimicrobial drug discovery through bacteriophage genomics. Nat. Biotechnol. 22:185-191.. OpenUrlCrossRefPubMedWeb of Science ...
Translocation and specific cleavage of bacteriophage T7 DNA in vivo by EcoKI | PNAS
... some plasmids and the phages T7 and T3 synthesize a direct inhibitor of type I restriction-modification systems (11-13). ... Bacteriophages and Bacteria.. T7 mutants sRK836 and the 0.3 deletion mutant D364 were kindly provided by F. W. Studier ( ... DNA Ejection from a Bacteriophage λ Particle Results in Immediate Cleavage of 0.3+ Phage DNA by EcoKI.. The above data suggest ... Translocation and specific cleavage of bacteriophage T7 DNA in vivo by EcoKI. L. René García and Ian J. Molineux ...
T7 RiboMAX Express: Generation of 27kb in vitro Transcripts in Minutes
Enzymes like bacteriophage SP6, T3 and T7 RNA polymerases have been used to produce synthetic RNA for a wide variety of ... Leary, S.L. et al. (1991) DNA-dependent RNA polymerase from bacteriophage T3 transcribes and amplifies an RNA template in vitro ... Chamberlin, M. et al. (1983) Isolation of bacterial and bacteriophage RNA polymerases and their use in synthesis of RNA in ... In vitro transcription using bacteriophage DNA-dependent RNA polymerases has been optimized to mediate the synthesis of large ...
Bacteriophage K1-5 Encodes Two Different Tail Fiber Proteins, Allowing It To Infect and Replicate on both K1 and K5 Strains of...
1997) Transcription termination by bacteriophage T3 and SP6 RNA polymerases at Rho-independent terminators. Can. J. Microbiol. ... 2000) Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. ... We isolated a bacteriophage, ΦK1-5, that is able to infect and grow on either K1 or K5 strains of E. coli. It appears that its ... 1996) Bacteriophage T4 host range is expanded by duplications of a small domain of the tail fiber adhesion. J. Mol. Biol. 258: ...
PhagePhagesEscherichiaGenomePolymerasesVirusesEnterobacteriaLigasePromotersProteinsAbstractBacteriaVirulentCapsidRecombinantGeneCharacterizationMolecular BiologyDouble-strandedHydrolaseYersiniaPseudomonasSequenceStrainsKlebsiellaInfectionGenomes1997EukaryoticCapsidsVivoBacillusClostridiumInitiationSingle-strandedGenusPlasmidOrganizationIsolateTailInfectMoleculesAnatomyColi strain
- Dietz A, Kössel H, Hausmann R (1985) On the evolution of the terminal redundancies of Klebsiella phage No. 11 and of coliphages T3 and T7. (springer.com)
- Escherichia virus T3, also called bacteriophage T3 and T3 phage, is a bacteriophage capable of infecting susceptible bacterial cells, including strains of Escherichia coli. (wikipedia.org)
- P1 is a temperate bacteriophage (phage) that infects Escherichia coli and a some other bacteria. (cbd.int)
- Enterobacteria phage T3 is a bacteriophage that infects Escherichia coli bacteria. (cbd.int)
- Bacteriophages have adapted to bacterial defense systems, devised strategies to evade these anti-phage mechanisms and restored their infective capacity. (springer.com)
- McGrath S, Fitzgerald GF, van Sinderen D. Identification and characterization of phage-resistance genes in temperate lactococcal bacteriophages. (springer.com)
- Here we introduce the Phage On Tap (PoT) protocol for the quick and efficient preparation of homogenous bacteriophage (phage) stocks. (peerj.com)
- Due to increasing interest for the use of bacteriophage (phage) in medical, industrial, and molecular settings, new approaches are required to quickly and efficiently produce high titer, homogenous, and purified phage stocks. (peerj.com)
- To further analyze the correct taxonomic position of this virus we have completed the sequence of its genome noting its very close similarity to Yersinia phage Berlin and coliphage T3. (biomedcentral.com)
- A bacteriophage ( / b æ k ˈ t ɪər i oʊ f eɪ dʒ / ), also known informally as a phage ( / f eɪ dʒ / ), is a virus that infects and replicates within Bacteria and Archaea . (wikipedia.org)
- As foundation, here we observe directly, for the first time, extreme, sheet-like outer shell thinness in a hyper-expanded capsid of phage T3. (beds.ac.uk)
- Phage therapy has been in vogue from 1930 until date as evidenced by works from Eliava Institute of Bacteriophages Microbiology Virology at Tbilisi, Georgia (Summers 2012). (researchsquare.com)
- BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. (bvsalud.org)
- Bacteriophage T7 is a virulent phage with an icosahedral head, a short noncontractile tail, and a dsDNA genome of 39937 bp. (ecoliwiki.org)
- DNA packaging of phages phi29, T3 and T7 sometimes produces incompletely packaged DNA with quantized lengths, based on gel electrophoretic band formation. (osti.gov)
- some plasmids and the phages T7 and T3 synthesize a direct inhibitor of type I restriction-modification systems ( 11 - 13 ). (pnas.org)
- It is now accepted that bacteriophages (phages) are the most abundant biological entities in most ecosystems and soda lakes are no exception, with studies conducted on Mono Lake placing viral abundance at 10 9 ml -1 , among the highest in natural aquatic environments [ 19 ]. (biomedcentral.com)
- Bacteriophages (or 'phages') shape microbial ecosystems by infecting and killing targeted bacterial species. (elifesciences.org)
- Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7. (bvsalud.org)
- the relationship of two Serratia phages to coli-dysentery phages T3, T7, and D44. (naver.com)
- Infection of Escherichia coli containing the type I restriction enzyme Eco KI by bacteriophage T7 0.3 mutants leads to restriction during the late stages of genome entry and during DNA replication. (pnas.org)
- A virulent double-stranded DNA bacteriophage, ΦK1-5, has been isolated and found to be capable of infecting Escherichia coli strains that possess either the K1 or the K5 polysaccharide capsule. (asm.org)
- For instance, the T7 bacteriophage preys on various strains of Escherichia coli , a type of bacteria often found in the human gut. (elifesciences.org)
- Rapid ejection of a 0.3 + T7 genome from a bacteriophage λ particle results in degradation of the infecting DNA by Eco KI, showing that the normal T7 DNA translocation process delays restriction. (pnas.org)
- In this study, we describe the biological characteristics, whole genome sequences, and annotations for two new isolates of the B. megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. (peerj.com)
- Comparative analysis of the BM5 and BM10 genome structures, in conjunction with other B. megaterium bacteriophages, revealed relatively high levels of sequence and organizational identity. (peerj.com)
- The genome of Kvp1, the first Kluyvera cryocrescens -specific bacteriophage, was sequenced using pyrosequencing (454 technology) at the McGill University and Genome Québec Innovation Centre. (biomedcentral.com)
- At 39,472 bp, the annotated genome revealed a closer relationship to coliphage T3 than T7 with Kvp1 containing homologs to T3 early proteins S-adenosyl-L-methionine hydrolase (0.3) and protein kinase (0.7). (biomedcentral.com)
- Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. (wn.com)
- Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome , and may have relatively simple or elaborate structures. (wikipedia.org)
- Genome sequence, structural proteins, and capsid organization of the cyanophage syn5: a "horned" bacteriophage of marine synechococcus. (purdue.edu)
- A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis. (medconditions.net)
- Using PCR-based methods, we assembled two wheat cDNA sequences, wheat-G and wheat-C, that encode T3/T7 bacteriophage-like RNA polymerases (RNAPs) sharing 45% amino acid identity. (nih.gov)
- It is structurally similar to both the T3 and T7 bacteriophage RNA polymerases, and all three have a conserved catalytic core at the C-terminus. (news-medical.net)
- Compared to the T3 and T7 bacteriophage polymerases, POLRMT requires auxiliary proteins to recognize the promoter. (news-medical.net)
- Abortive initiation is typically studied in the T3 and T7 RNA polymerases in bacteriophages and in E. coli. (wikipedia.org)
- The most studied polymerases belong to Family A (found in prokaryotes, eukaryotes and bacteriophages) and family B (found in prokaryotes, eukaryotes, archaea, and viruses). (frontiersin.org)
- Bacteria can cause diseases, but they also battle their own microscopic enemies: a group of viruses known as bacteriophages. (elifesciences.org)
- Bacteriophages are probably the oldest viruses, having appeared early during bacterial evolution. (springer.com)
-  Bacteriophages are ubiquitous viruses, found wherever bacteria exist. (wikipedia.org)
- This tightly regulated transcriptional pattern of SSV1 has not been described before for any archaeal virus and is reminiscent of those of many bacteriophages and some eukaryotic viruses. (tu-darmstadt.de)
- To unravel the influence of viruses, and particularly bacteriophages, on microbiota ecology and animal host physiology and health, methods allowing quantitative comparison of virus diversity, abundance and function across samples are needed. (biomedcentral.com)
- Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. (medconditions.net)
- DNA_ligase_ATP-dep_T3. (genome.jp)
- T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. (neb.com)
- Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase (1). (neb.com)
- T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity. (neb.com)
- An E. coli strain containing a recombinant gene encoding T3 DNA Ligase. (neb.com)
- One unit is defined as the amount of enzyme required to give 50% ligation of 100 ng HindIII fragments of λ DNA in a total reaction volume of 20 μl in 1 minute at 25°C in 1X T3 DNA Ligase Reaction Buffer. (neb.com)
- T3 DNA Ligase is also active in buffers without PEG 6000, such as our T4 DNA Ligase Buffer and NEBuffers 1-4, for applications in which PEG 6000 is detrimental. (neb.com)
- T3 DNA ligase , which can also ligate both blunt and sticky-ended DNA fragments, can tolerate salt concentrations of up to 250-300 mM. (bitesizebio.com)
- In these buffers T3 DNA Ligase exhibits an approximately 10-fold reduction in activity. (neb.com)
- Heating a reaction containing T3 DNA Ligase at 65°C for 10 minutes will inactivate the enzyme. (neb.com)
- The DNA sequences of three major class III T3 RNA polymerase promoters located at 45.0, 55.0, and 64.8% on the standard T3 genetic map have been determined. (elsevier.com)
- Alignment of these three class III promoters and a previously determined T3 RNA polymerase promoter at 1.05% on T3 genetic map, with start points of transcription (+1) in register, indicates a high degree of sequence conservation among the four T3 RNA polymerase promoters. (elsevier.com)
- Furthermore, careful examination of the nucleotide sequences around 45.0 and 64.8 T3 map units shows that the putative RNA sequences arising from these regions by overlapping transcription from upstream promoters can be arranged into stable stemloop structures thought to be required for RNase III cleavage. (elsevier.com)
- The bacteriophage promoters, T7, T3, and SP6, consist of 23 basepairs numbered -17 to +6, where +1 indicates the first base of the coded transcript. (thermofisher.com)
- Neurons expressing glutamic acid decarboxylase (GAD) mRNA were localized by in situ hybridization in normal and monocularly deprived cat visual cortex by using single-stranded RNA probes transcribed from cDNAs cloned in vectors with the T3 and T7 RNA polymerase promoters. (elsevier.com)
- and (iv T7 and T3 bacteriophage promoters and EcoRI, NotI and BamHI restriction sites flanking the cloning site that allow for synthesis of, or isolation of, end-specific probes for chromosome walking. (elsevier.com)
- It also encodes the beta galactosidase alpha peptide for bluewhite color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. (lifescience-market.com)
- By regulating the expression of the rat liver SAM synthetase and the bacteriophage T3 SAM hydrolase proteins in E. coli , a 100-fold range of SAM levels could be achieved. (asm.org)
- This is the first report to demonstrate that membrane-bound proteins are involved in the adsorption of a T7-related bacteriophage. (asm.org)
- Abstract&list_uids=12079351&itool=iconabstr&query_hl=13&itool=pubmed_docsum 'Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3. (openwetware.org)
- Although well studied with respect to their microbial composition, their viral compositions have not, and consequently few bacteriophages that infect bacteria from haloalkaline environments have been described. (biomedcentral.com)
- Therefore, bacteria and bacteriophages have a long history of co-evolution in which bacteria have developed multiple resistance mechanisms against bacteriophages. (springer.com)
- It is estimated there are more than 10 31 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. (wikipedia.org)
- Bacteriophages are a promising alternative for curtailing infections caused by multi drug resistant (MDR) bacteria. (researchsquare.com)
- BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. (bvsalud.org)
- During the morphogenesis of double-stranded DNA bacteriophages, a DNA-free protein capsid (procapsid) is assembled and subsequently packages the bacteriophage DNA. (uthscsa.edu)
- Similar in structure to T3, each virion of T7 has an icosahedral head (capsid) with a diameter of 60-61 nm and a 2 nm thick shell (the outer layer being made from two forms of the gene 10 protein). (ecoliwiki.org)
- 100. The expression library of claim 91 wherein the expression vector is a bacteriophage vector and wherein the expression library comprises recombinant bacteriophage particles. (patentsencyclopedia.com)
- The deduced amino acid sequence of this polypeptide shows 71% homology to the T7 RNA polymerase (the product of T7 gene 1 ), 72% homology to the T3 RNA polymerase and 27% homology to the SP6 RNA polymerase. (springer.com)
- Kotani H, Ishizaki Y, Hiraoka N, Obayashi A (1987) Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase. (springer.com)
- Moffatt BA, Dunn JJ, Studier FW (1984) Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. (springer.com)
- 1986) Bacteriophage T3 gene 8 product oligomer structure. (forexsignalx.com)
- Characterization of the lysogeny DNA module from teteperate Streptococcus thermophilus bacteriophage φSfi21. (springer.com)
- Bacteriophage Genetics and Molecular Biology. (springer.com)
- Head morphogenesis of complex double-stranded deoxyribonucleic acid bacteriophages. (naver.com)
- In this study, we used the regulated expression of two foreign enzymes to evade E. coli 's stringent regulation of SAM levels, namely, expression of rat liver SAM synthetase (RLSS) and bacteriophage T3 SAM hydrolase (T3SH). (asm.org)
- In E. coli MG1655, expression of T3SH [the bacteriophage T3 S-adenosylmethionine (SAM) hydrolase] significantly reduced AI-2 activity in culture supernatants, suggesting that AI-2 production is limited by the amount of SAH produced in SAM-dependent transmethylase reactions. (lancs.ac.uk)
- Yep-phi is a T7-related bacteriophage specific to Yersinia pestis , and it is routinely used in the identification of Y. pestis in China. (asm.org)
- Krylov, V. 2006-03-01 00:00:00 The T7-like ΦKMV bacteriophage active on Pseudomonas aeruginosa was previously isolated by us and shown to have DNA resistant to many endonucleases. (deepdyve.com)
- Commercial production of bacteriophages specific against Staphylococcus spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Shigella spp were done till mid 1950's at EIBMV, HIEET, Poland (Sulakvelidze et al. (researchsquare.com)
- Dunn JJ, Studier FW (1983) The complete nucleotide sequence of bacteriophage T7 dNA and the locations of T7 genetic elements. (springer.com)
- RT "Sequence of a conditionally essential region of bacteriophage T3, RT including the primary origin of DNA replication. (genome.jp)
- A study in evolution: the DNA base sequence homology between coliphages T7 and T3. (naver.com)
- ΦK5 is a related bacteriophage specific for E. coli strains that display the K5 antigen, a polymer consisting of a repeating structure of 4-linked α- N -acetylglucosamine and β-glucuronic acid ( N -acetyl heparosin). (asm.org)
- Monitoring the bacteriophages that survived and multiplied the most after infecting different strains of E. coli revealed which RBP building blocks are important for efficiency and specificity. (elifesciences.org)
- Diverse Temperate Bacteriophage Carriage in Clostridium difficile 027 Strains," PLoS ONE (May 2012), 7(5):e372631-9. (patents.com)
- Rudolph C, Freund-Mölbert E, Stirm S (1975) Fragments of Klebsiella bacteriophage No. 11. (springer.com)
- Bacteriophages occur abundantly in the biosphere, with different genomes, and lifestyles. (wikipedia.org)
- 1997) 272, 21±30 Positioning of the Start Site in the Initiation of Transcription by Bacteriophage T7 RNA Polymerase Benjamin F. Weston, Iaroslav Kuzmine and Craig T. Martin* Department of Chemistry University of Massachusetts Amherst, MA , USA *Corresponding author The determination of various polymerase structures has sparked interest in understanding how the polynucleotide template interacts with the active site. (docplayer.net)
- In recent years, advances in sequencing technologies and data analysis techniques have enabled detailed metagenomic studies investigating intestinal viromes (collections of bacteriophage and eukaryotic viral nucleic acids) and their potential contributions to the ecology of the microbiota. (biomedcentral.com)
- Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. (peerj.com)
- The lack of information on bacteriophages of Clostridium difficile prompted this study. (asm.org)
- Bacteriophage treatment significantly reduces viable Clostridium difficile and prevents toxin production in an in vitro model system," Anaerobe (2010), 16:549-554. (patents.com)
- Prevention of Clostridium difficile-induced ileocecitis with Bacteriophage," Anaerobe (1999), 5:69-78. (patents.com)
- 1. A method of treatment comprising administering to a patient a pharmaceutical composition comprising two or more bacteriophages selected from the group consisting of NCTC 12081404, NCTC 12081405, NCTC 12081406, NCTC 12081407, NCTC 12081408, NCTC 12081409, and NCTC 12081410, wherein the two or more bacteriophages are capable of lysing more than 5 different ribotypes of Clostridium difficile, and wherein the 5 different ribotypes comprise 014/20 or 027. (patents.com)
- Here, we investigate this replication re-initiation pathway using the bacteriophage T7 replisome. (nature.com)
- Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. (springer.com)
- The quantitative nature of the relationships between Kvp1 and the other members of the T7-like virus genus (T7, T3, φA1122, φYeO3-12, Berlin, K1F, VP4 and gh-1) was confirmed using CoreGenes. (biomedcentral.com)
- CC The order of the major features in this plasmid is: TRP1 - f1 ori CC (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CC CEN6 - ARSH4. (stanford.edu)
- The order of the major features in this plasmid is: LEU2 f1 ori (NaeI) T7 promoter lacZ/MCS T3 promoter pMB1 ori bla CEN6 ARSH4. (lifescience-market.com)
- 95. The expression library of claim 91 wherein the expression vector is a plasmid vector, bacteriophage vector or phagemid vector. (patentsencyclopedia.com)
- The aim of this study, therefore, was to isolate and characterize bacteriophages specific for C. difficile as a preliminary step to assessing their potential as novel therapeutic agents. (asm.org)
- These findings demonstrate that even small changes to the bacteriophages can make a big difference to their ability to infect their preys. (elifesciences.org)
- Terminal repetition in non-permuted T3 and T7 bacteriophage DNA molecules. (naver.com)
- The anatomy of the T5 bacteriophage DNA molecule (1966) Abelson John et al. (naver.com)
- This was then confirmed by engineering highly active T7 bacteriophage variants against an E. coli strain that causes urinary tract infections. (elifesciences.org)