Bacteriophage Pf1
Pseudomonas Phages
Bacteriophage T4
Bacteriophage lambda
Bacteriophage T7
Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
T-Phages
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
Bacteriophage mu
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Bacteriophage phi 6
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bacteriophage phi X 174
Bacteriophage P2
Bacteriophage M13
Bacteriophage T3
Bacteriophage Typing
Bacteriophage P1
Salmonella Phages
Siphoviridae
RNA Phages
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Bacteriolysis
Bacteriophage PRD1
Bacillus Phages
Base Sequence
Mutation
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Viral Tail Proteins
Levivirus
Adsorption
DNA Packaging
Plasmids
Prophages
Inovirus
PubMed
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Models, Molecular
Amyloid
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
Crystallization
Protein Structure, Secondary
Cell death in Pseudomonas aeruginosa biofilm development. (1/14)
Bacteria growing in biofilms often develop multicellular, three-dimensional structures known as microcolonies. Complex differentiation within biofilms of Pseudomonas aeruginosa occurs, leading to the creation of voids inside microcolonies and to the dispersal of cells from within these voids. However, key developmental processes regulating these events are poorly understood. A normal component of multicellular development is cell death. Here we report that a repeatable pattern of cell death and lysis occurs in biofilms of P. aeruginosa during the normal course of development. Cell death occurred with temporal and spatial organization within biofilms, inside microcolonies, when the biofilms were allowed to develop in continuous-culture flow cells. A subpopulation of viable cells was always observed in these regions. During the onset of biofilm killing and during biofilm development thereafter, a bacteriophage capable of superinfecting and lysing the P. aeruginosa parent strain was detected in the fluid effluent from the biofilm. The bacteriophage implicated in biofilm killing was closely related to the filamentous phage Pf1 and existed as a prophage within the genome of P. aeruginosa. We propose that prophage-mediated cell death is an important mechanism of differentiation inside microcolonies that facilitates dispersal of a subpopulation of surviving cells. (+info)Prediction of charge-induced molecular alignment of biomolecules dissolved in dilute liquid-crystalline phases. (2/14)
Alignment of macromolecules in nearly neutral aqueous lyotropic liquid-crystalline media such as bicelles, commonly used in macromolecular NMR studies, can be predicted accurately by a steric obstruction model (Zweckstetter and Bax, 2000). A simple extension of this model is described that results in improved predictions for both the alignment orientation and magnitude of protein and DNA solutes in charged nematic media, such as the widely used medium of filamentous phage Pf1. The extended model approximates the electrostatic interaction between a solute and an ordered phage particle as that between the solute's surface charges and the electric field of the phage. The model is evaluated for four different proteins and a DNA oligomer. Results indicate that alignment in charged nematic media is a function not only of the solute's shape, but also of its electric multipole moments of net charge, dipole, and quadrupole. The relative importance of these terms varies greatly from one macromolecule to another, and evaluation of the experimental data indicates that these terms scale differently with ionic strength. For several of the proteins, the calculated alignment is sensitive to the precise position of the charged groups on the protein surface. This suggests that NMR alignment measurements can potentially be used to probe protein electrostatics. Inclusion of electrostatic interactions in addition to steric effects makes the extended model applicable to all liquid crystals used in biological NMR to date. (+info)Therapy of experimental pseudomonas infections with a nonreplicating genetically modified phage. (3/14)
Bacteriophage therapy of bacterial infections has received renewed attention owing to the increasing prevalence of antibiotic-resistant pathogens. A side effect of many antibiotics as well as of phage therapy with lytic phage is the release of cell wall components, e.g., endotoxins of gram-negative bacteria, which mediate the general pathological aspects of septicemia. Here we explored an alternative strategy by using genetically engineered nonreplicating, nonlytic phage to combat an experimental Pseudomonas aeruginosa infection. An export protein gene of the P. aeruginosa filamentous phage Pf3 was replaced with a restriction endonuclease gene. This rendered the Pf3 variant (Pf3R) nonreplicative and concomitantly prevented the release of the therapeutic agent from the target cell. The Pf3R phage efficiently killed a wild-type host in vitro, while endotoxin release was kept to a minimum. Treatment of P. aeruginosa infections of mice with Pf3R or with a replicating lytic phage resulted in comparable survival rates upon challenge with a minimal lethal dose of 3. However, the survival rate after phage therapy with Pf3R was significantly higher than that with the lytic phage upon challenge with a minimal lethal dose of 5. This higher survival rate correlated with a reduced inflammatory response elicited by Pf3R treatment relative to that with the lytic phage. Therefore, this study suggests that the increased survival rate of Pf3R-treated mice could result from reduced endotoxin release. Thus, the use of a nonreplicating modified phage for the delivery of genes encoding proteins toxic to bacterial pathogens may open up a new avenue in antimicrobial therapy. (+info)The polybasic region that follows the plant homeodomain zinc finger 1 of Pf1 is necessary and sufficient for specific phosphoinositide binding. (4/14)
The plant homeodomain (PHD) zinc finger is one of 14 known zinc-binding domains. PHD domains have been found in more than 400 eukaryotic proteins and are characterized by a Cys(4)-His-Cys(3) zinc-binding motif that spans 50-80 residues. The precise function of PHD domains is currently unknown; however, the PHD domains of the ING1 and ING2 tumor suppressors have been shown recently to bind phosphoinositides (PIs). We have recently identified a novel PHD-containing protein, Pf1, as a binding partner for the abundant and ubiquitous transcriptional corepressor mSin3A. Pf1 contains two PHD zinc fingers, PHD1 and PHD2, and functions to bridge mSin3A to the TLE1 corepressor. Here, we show that PHD1, but not PHD2, binds several monophosporylated PIs but most strongly to PI(3)P. Surprisingly, a polybasic region that follows the PHD1 is necessary for PI(3)P binding. Furthermore, this polybasic region binds specifically to PI(3)P when fused to maltose-binding protein, PHD2, or as an isolated peptide, demonstrating that it is sufficient for specific PI binding. By exchanging the polybasic regions between different PHD fingers we show that this region is a strong determinant of PI binding specificity. These findings establish the Pf1 polybasic region as a phosphoinositide-binding module and suggest that the PHD domains function down-stream of phosphoinositide signaling triggered by the interaction between polybasic regions and phosphoinositides. (+info)Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. (5/14)
(+info)Solid-state NMR spectroscopy of a membrane protein in biphenyl phospholipid bicelles with the bilayer normal parallel to the magnetic field. (6/14)
(+info)Liquid crystalline phase of G-tetrad DNA for NMR study of detergent-solubilized proteins. (7/14)
(+info)Conformational dynamics of an intact virus: order parameters for the coat protein of Pf1 bacteriophage. (8/14)
(+info)
DNA sequence of the filamentous bacteriophage Pf1. - PubMed - NCBI
Membrane association and functional regulation of Sec3 by phospholipids and Cdc42 | Journal of Cell Biology | Rockefeller...
Hybridization of TEDOR and NCX MAS solid-state NMR experiments for simultaneous acquisition of heteronuclear correlation...
Expression of Foreign Genes in the Pseudomonas Bacteriophage Pf3 by Krystin Weathers
Chemistry of 1,3-dioxepins. XIII. (|i|E|/i|)/(|i|Z|/i|) Configurational assignment of 4,7-dihydro-4-hydroxyimino-6-nitro-1,3...
A Thorough Dynamic Interpretation of Residual Dipolar Couplings in Ubiquitin | SpringerLink
RCSB PDB - 2KSJ: Structure and Dynamics of the Membrane-bound form of Pf1 Coat Protein: Implications for Structural...
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PF1
... , PF-1, PF01, PF-01, or variant, may refer to: PSA PF1 platform, the "PF1" platform from PSA pf1, a bacteriophage code, see ... PF-1), "PF-1" ship number Thai patrol frigate HTMS Tachin Shenyang PF-1, model PF-1 jet engine from Shenyang MANOI PF01, model ... model PF-1 glider "White Knight" from Posnansky/Fronius USS Asheville (PF-1), "PF-1" ship number USN patrol frigate USS ... List of MeSH codes (B04) Pf1, a Pseudomonas phage, see List of viruses Posnansky/Fronius PF-1 White Knight, ...
Filamentous bacteriophage
... species Pseudomonas virus Pf1 Pf1 phage genus Tertilicivirus) species Pseudomonas virus Pf3 - bacteriophages that infect ... species Escherichia virus M13 M13 bacteriophage f1 phage species Filamentous bacteriophage fd (proposal) fd phage genus ... whereas Class II includes strains Pf1 (of ICTV's species Pseudomonas virus Pf1 of genus Primolicivirus), and perhaps also Pf3 ( ... inactivated infectivity as predicted for a filamentous bacteriophage morphology. Three filamentous bacteriophages, fd, f1 and ...
List of MeSH codes (B04)
... bacteriophage phi x 174 MeSH B04.123.660.535 - bacteriophage pf1 MeSH B04.123.660.550 - bacteriophage phi 6 MeSH B04.123. ... bacteriophage ike MeSH B04.123.370.400.250 - bacteriophage m13 MeSH B04.123.370.400.300 - bacteriophage pf1 MeSH B04.123. ... bacteriophage ike MeSH B04.280.400.400.250 - bacteriophage m13 MeSH B04.280.400.400.300 - bacteriophage pf1 MeSH B04.280. ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
Polynucleotide adenylyltransferase
... filters-fiberglass-HeLa cells-bacteriophage T4)". Proc. Natl. Acad. Sci. U.S.A. 69 (2): 417-21. doi:10.1073/pnas.69.2.417. PMC ... hydrolase RNA formation factors PF1 Adenosine triphosphate:ribonucleic acid adenylyltransferase This enzyme is responsible for ...
DNA sequence of the filamentous bacteriophage Pf1. - PubMed - NCBI
DNA sequence of the filamentous bacteriophage Pf1.. Hill DF1, Short NJ, Perham RN, Petersen GB. ... The genome of the class II filamentous bacteriophage Pf1 has been sequenced by a combination of the chain termination and ... The Pf1 genome contains no collection of potential stem-and-loop structures corresponding to those that initiate replication of ... Overall, the organization of the Pf1 genome differs from that of the other class II filamentous phage whose genome has been ...
RCSB PDB
for 4IFM
Pf1 filamentous bacteriophage: refinement of a molecular model by simulated annealing using 3.3 A resolution X-ray fibre ... PF1 FILAMENTOUS BACTERIOPHAGE A 46 Pseudomonas virus pf1 Details: MAJOR COAT PROTEIN ASSEMBLY IN THE LOWER-TEMPERATURE SYMMETRY ... PF1 FILAMENTOUS BACTERIOPHAGE: REFINEMENT OF A MOLECULAR MODEL BY SIMULATED ANNEALING USING 3.3 ANGSTROMS RESOLUTION X-RAY ...
NMR structures of membrane proteins in phospholipid bilayers | Quarterly Reviews of Biophysics | Cambridge Core
Nambudripad, R., Stark, W., Opella, S. J. & Makowski, L. (1991). Membrane-mediated assembly of filamentous bacteriophage Pf1 ... Thiriot, D. S., Nevzorov, A. A. & Opella, S. J. (2005). Structural basis of the temperature transition of Pf1 bacteriophage. ... NMR studies of the structure and dynamics of membrane-bound bacteriophage Pf1 coat protein. Science 252, 1303-1305. ... Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy. Journal of Molecular Biology 341 ...
PA0721 - Pseudomonas phage Pf1
RCSB PDB - Launch Viewer
for 2IFN
Cell Death in Pseudomonas aeruginosa Biofilm Development | Journal of Bacteriology
The bacteriophage implicated in biofilm killing was closely related to the filamentous phage Pf1 and existed as a prophage ... We found evidence of wild-type RF molecules in the Pf1::Gmr strain by using PCR primers Pf1 3 and Pf1 4, which amplify a region ... A Pf1::Gmr cassette was constructed by (i) PCR amplifying Pf1 genes from genomic DNA of P. aeruginosa PAO1 with primers Pf1 3 ( ... By using the Pf1-specific PCR primers 437F and 437R, which recognize Pf1 open reading frame 437, we detected and confirmed by ...
Overview: PA0717, Pseudomonas aeruginosa PAO1
Predicted Orthologs - RBBH
coat protein B of bacteriophage Pf1 Pseudomonas aeruginosa UCBPP-PA14 PA14_48940 coat protein B of bacteriophage Pf1) 82 92.7 ... probable coat protein A of bacteriophage Pf1 Pseudomonas aeruginosa UCBPP-PA14 PA14_48930 coat protein A of bacteriophage Pf1 ... hypothetical protein of bacteriophage Pf1 Pseudomonas aeruginosa UCBPP-PA14 PA14_49000 hypothetical protein 70 95.7 ... hypothetical protein of bacteriophage Pf1 Pseudomonas aeruginosa UCBPP-PA14 PA14_48990 hypothetical protein 96 96.9 ...
Frontiers | Challenges and Promises for Planning Future Clinical Research Into Bacteriophage Therapy Against Pseudomonas...
Century-long research has suggested treating patients with bacteriophages (phages, prokaryotic viruses) naturally hosted by ... Century-long research has suggested treating patients with bacteriophages (phages, prokaryotic viruses) naturally hosted by ... Small colony variants and single nucleotide variations in Pf1 region of PB1 phage-resistant Pseudomonas aeruginosa. Front. ... Ackermann, H. W. (2005). "Bacteriophage classification," in Bacteriophages: Biology and Applications, eds E. Kutter, and A. ...
Publikationen | Max-Planck-Institut für biophysikalische Chemie
Degree Level: Doctoral / Type: Theses / Subject: Biophysics | Search Results | Academic Commons
Type: Theses / Subject: Chemistry, Physical and theoretical | Search Results | Academic Commons
Paul Bollyky | Stanford Medicine Profiles
Bacteriophage / Human Immune Interactions. We recently reported that bacteriophage present at sites of bacterial infection are ... Two other phages, Pf1 and fd, did not inhibit Af, nor did supernatant from a Pa strain incapable of producing Pf4. Pf4, but not ... Fd, a related bacteriophage of Escherichia coli, has similar biofilm-building capabilities. Targeting filamentous bacteriophage ... 2019 sought to kill cancer-causing bacteria with bacteriophages. However, the bacteriophages themselves directly stimulated an ...
Alfred M. Spormann | Stanford Medicine Profiles
Two other phages, Pf1 and fd, did not inhibit Af, nor did supernatant from a Pa strain incapable of producing Pf4. Pf4, but not ... Here, we report that the bacteriophage Pf4, produced by Pa, can inhibit the metabolic activity of Af biofilms. This phage- ... Pf4 bacteriophage produced by Pseudomonas aeruginosa inhibits Aspergillus fumigatus metabolism via iron sequestration ... Pf1, attaches to Af hyphae in an avid and prolonged manner, suggesting that Pf4-mediated inhibition of Af may occur at the ...
Intrinsically disordered γ-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary...
DNP-NMR - Nuclear Magnetic Resonance | Bruker
PF1 - Wikipedia
PF1, PF-1, PF01, PF-01, or variant, may refer to: PSA PF1 platform, the "PF1" platform from PSA pf1, a bacteriophage code, see ... PF-1), "PF-1" ship number Thai patrol frigate HTMS Tachin Shenyang PF-1, model PF-1 jet engine from Shenyang MANOI PF01, model ... model PF-1 glider "White Knight" from Posnansky/Fronius USS Asheville (PF-1), "PF-1" ship number USN patrol frigate USS ... List of MeSH codes (B04) Pf1, a Pseudomonas phage, see List of viruses Posnansky/Fronius PF-1 White Knight, ...
Filamentous bacteriophage - Wikipedia
... species Pseudomonas virus Pf1 Pf1 phage genus Tertilicivirus) species Pseudomonas virus Pf3 - bacteriophages that infect ... species Escherichia virus M13 M13 bacteriophage f1 phage species Filamentous bacteriophage fd (proposal) fd phage genus ... whereas Class II includes strains Pf1 (of ICTVs species Pseudomonas virus Pf1 of genus Primolicivirus), and perhaps also Pf3 ( ... inactivated infectivity as predicted for a filamentous bacteriophage morphology. Three filamentous bacteriophages, fd, f1 and ...
Lynette Cegelski's Profile | Stanford Profiles
The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. ... To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca ... Bacteriophage-fungal interactions may be a general feature with several pathogens in the fungal kingdom. ...
PDB: 1RGO
Small Colony Variants and Single Nucleotide Variations in Pf1 Region of PB1 Phage-Resistant Pseudomonas aeruginosa - Semantic...
In this study, SCVs was derived from Pseudomonas aeruginosa exposed to the lytic bacteriophage PB1 and these cells were ... Whole genome sequencing of the SCV revealed multiple single nucleotide variations within the Pf1 prophage region, a genetic ... Phage therapy involves the application of lytic bacteriophages for treatment of clinical infections but bacterial resistance ... Bacteriophage therapy for refractory Pseudomonas aeruginosa urinary tract infection.. Abdel Karim Khawaldeh, Sandra Morales, +7 ...
DeCS
Bacteriophage Pf1 - Preferred Concept UI. M0027063. Scope note. A species of filamentous Pseudomonas phage in the genus ... Pf1 Phage Pf1 Phages Phage Pf1 Phage, Pf1 Phages, Pf1 Pseudomonas phage Pf1 ... Pf1 Phage. Pf1 Phages. Phage Pf1. Phage, Pf1. Phages, Pf1. Pseudomonas phage Pf1. ... Bacteriophage Pf1 Entry term(s). ...
New bacteriophages that infect the phytopathogen Ralstonia solanacearum | Microbiology Society
Four kinds of bacteriophage (φRSL, φRSA, φRSM and φRSS) were isolated from Ralstonia solanacearum, a soil-borne Gram-negative ... Hill, D. F., Short, J., Perharm, N. R. & Petersen, G. B. ( 1991; ). DNA sequence of the filamentous bateriophage Pf1. J Mol ... Model, P. & Russel, M. ( 1988; ). Filamentous bacteriophages. In The Bacteriophages, vol. 2, pp. 375-456. Edited by R. Calendar ... Four kinds of bacteriophage (φRSL, φRSA, φRSM and φRSS) were isolated from Ralstonia solanacearum, a soil-borne Gram-negative ...
Overview: PA0719, Pseudomonas aeruginosa PAO1
1QL1 | Genus
Polyelectrolyte-mediated increase of biofilm mass formation | BMC Microbiology | Full Text
The bacteriophage Pf1 (1 mg/ml) significantly increased biofilm mass produced by Pseudomonas aeruginosa P14, Escherichia coli ... Addition of F-actin, DNA or Pf1 bacteriophage to 0.5% agar plates increased swarming motility of Pseudomonas aeruginosa Xen5. ... DNA, F-actin, NFs and Pf1 also increased biofilm mass of the fungal C. albicans 1409 strain. ... vimentin and purified Pf1 bacteriophage on biofilm formation and swarming motility of select pathogens including Pseudomonas ...
PROCESS OF PRODUCTION OF BACTERIOPHAGE COMPOSITIONS AND METHODS IN PHAGE THERAPY FIELD - Patent application
... bacteriophages ike, m13 and pf1. The plectrovirus genus comprises bacteriophages that infect Acholeplasma and Spiroplasma. [ ... bacteriophage is related to the Salmonella typhimurium bacteriophage MB78. The growth of φmF bacteriophage has been tested on ... Such bacteriophage can be selected as described previously. [0065]Determining an effective amount of the bacteriophage to be ... 0046]We use any bacteriophage, preferably bacteriophages that are virulent for the bacterial strain used in the method. As an ...
PROTEINS OF KNOWN STRUCTURE BY SOLID-STATE NMR
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International Tables for Crystallography) Polymer crystallography
Makowski, L., Caspar, D. L. D. & Marvin, D. A. (1980). Filamentous bacteriophage Pf1 structure determined at 7 Å resolution by ... Gonzalez, A., Nave, C. & Marvin, D. A. (1995). Pf1 filamentous bacteriophage: refinement of a molecular model by simulated ... Stark, W., Glucksman, M. J. & Makowski, L. (1988). Conformation of the coat protein of filamentous bacteriophage Pf1 determined ... Nambudripad, R., Stark, W. & Makowski, L. (1991). Neutron diffraction studies of the structure of filamentous bacteriophage Pf1 ...
Phage15
- Overall, the organization of the Pf1 genome differs from that of the other class II filamentous phage whose genome has been sequenced, Pf3, as much as it does from that of the class I phages Ff and IKe. (nih.gov)
- The bacteriophage implicated in biofilm killing was closely related to the filamentous phage Pf1 and existed as a prophage within the genome of P. aeruginosa . (asm.org)
- Current efforts are focused on understanding how bacteriophage interact with mammalian cells, how this infection influences local immunity, and how phage shape the immune response to commensal and pathogenic bacteria. (stanford.edu)
- Filamentous bacteriophages are among the simplest living organisms known, with far fewer genes than the classical tailed bacteriophages studied by the phage group. (wikipedia.org)
- This technical difference has little noticeable effect on the overall phage structure, but the extent of independent diffraction data is greater for symmetry Class II than for Class I. This assisted the determination of the Class II phage Pf1 structure, and by extension the Class I structure. (wikipedia.org)
- inproceedings{Lim2016SmallCV, title={Small Colony Variants and Single Nucleotide Variations in Pf1 Region of PB1 Phage-Resistant Pseudomonas aeruginosa}, author={Wee Shiong Lim and Kevin K. S. Phang and Andy H.-M. Tan and Sam F.-Y. Li and Dave S.-W. Ow}, booktitle={Front. (semanticscholar.org)
- Phage therapy involves the application of lytic bacteriophages for treatment of clinical infections but bacterial resistance may develop over time. (semanticscholar.org)
- In this study, SCVs was derived from Pseudomonas aeruginosa exposed to the lytic bacteriophage PB1 and these cells were resistant to subsequent phage infection by PB1. (semanticscholar.org)
- The Pf1 phage exists largely as bundles of long filamentous phages extending from the bacterial surface [ 19 ]. (biomedcentral.com)
- Pseudomonas phage Pf1 NMR the suburbs and 5 to the country are using magnetically aligned bacteriophage. (laurenrossimakeup.com)
- Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. (vcu.edu)
- Whole genome sequencing revealed several genetic variations between the BIM and phage-sensitive isolate of each strain, most notably in the prophage Pf1 region of the genome. (edu.au)
- In summary, bacteriophage therapy holds promise for treating P. aeruginosa infections in CRS and CF. Specifically, the CT-PA phage cocktail appears to be safe and effective in treating P. aeruginosa sinus infections in an in vivo animal model. (edu.au)
- Further investigations are required to determine whether the development of BIMs will hamper the efficacy of lytic bacteriophage therapy, or conversely whether the emergence of phage resistance may create evolutionary trade-offs that can be exploited to decrease resistance to conventional antibiotics. (edu.au)
- Non-synonymous SNPs were mainly found in an integrated Pf1-like phage and in genes involved in transcriptional regulation, membrane and extracellular constituents, transport, and secretion. (frontiersin.org)
Filamentous7
- DNA sequence of the filamentous bacteriophage Pf1. (nih.gov)
- The genome of the class II filamentous bacteriophage Pf1 has been sequenced by a combination of the chain termination and chemical degradation methods. (nih.gov)
- The size and position of its open reading frames (ORFs) in general resemble those of other filamentous bacteriophage genomes. (nih.gov)
- Filamentous bacteriophage is a family of viruses (Inoviridae) that infect bacteria. (wikipedia.org)
- Three filamentous bacteriophages, fd, f1 and M13, were isolated and characterized by three different research groups in the early 1960s, but they are so similar that they are sometimes grouped under the common name "Ff", which are members of genus Inovirus, as acknowledged by the International Committee on Taxonomy of Viruses (ICTV). (wikipedia.org)
- The primary immunity determinant in modulating the lysogenic immunity of the filamentous bacteriophage cf. (microbiologyresearch.org)
- X-ray fibre diffraction patterns of well-aligned Pf1 filamentous bacteriophage show sharp layer-lines attributable to an ordered helical array of protein subunits. (kneegasp.top)
Infect2
- The effects of Pf1 on biofilm formation by bacterial strains that it cannot infect suggests that the physicochemical properties of Pf1 and similar polyelectrolytes, rather than any specific biological effect of the bacteriophage on the bacteria is required for it to support biofilm formation by different microorganisms. (biomedcentral.com)
- Lytic bacteriophages are bacterial viruses that can infect, replicate within, and lyse bacteria, killing the host bacteria through lysis. (edu.au)
Capsid1
- The NMR-Rosetta capsid model of M13 bacteriophage reveals a quadrupled hydrophobic packing epitope. (semanticscholar.org)
Biofilm7
- During the onset of biofilm killing and during biofilm development thereafter, a bacteriophage capable of superinfecting and lysing the P. aeruginosa parent strain was detected in the fluid effluent from the biofilm. (asm.org)
- This study was designed to assess the impact of diverse natural polyelectrolytes, such as DNA, F-actin, neurofilaments (NFs), vimentin and purified Pf1 bacteriophage on biofilm formation and swarming motility of select pathogens including Pseudomonas aeruginosa associated with lung infections in CF patients. (biomedcentral.com)
- The bacteriophage Pf1 (1 mg/ml) significantly increased biofilm mass produced by Pseudomonas aeruginosa P14, Escherichia coli RS218 and Bacillus subtilis ATCC6051. (biomedcentral.com)
- DNA, F-actin, NFs and Pf1 also increased biofilm mass of the fungal C. albicans 1409 strain. (biomedcentral.com)
- The PA strain PAO1 strongly up-regulates genes encoding Pf1-like phages at the start of biofilm formation, and Pf1-like virions are present at 100 to 1000 fold higher levels in biofilms when compared with planktonic PA cultures [ 18 ]. (biomedcentral.com)
- This report indicates that addition of diverse natural polyelectrolytes, including purified Pf1 bacteriophage, to different bacteria or Candida albicans cultures results in increased biofilm formation. (biomedcentral.com)
- The emergence of bacteriophage insensitive mutants (BIMs) in P. aeruginosa biofilms exposed to CT-PA was also explored in an in vitro biofilm model, using whole genome sequencing and antibiotic susceptibility testing. (edu.au)
Genome1
- The Pf1 genome contains no collection of potential stem-and-loop structures corresponding to those that initiate replication of Ff DNA and assembly of the Ff virion, although isolated structures of this kind are present. (nih.gov)
Strain2
- 2. The method according to claim 1, wherein the concentration of said antibiotic in the medium is in the range which causes about 1% to about 99% inhibition of the growth of said bacterial strain in the absence of said bacteriophage. (patentsencyclopedia.com)
- From the compatible group of PGPR, we have selected one biofertilizer (Azospirillum brasilense strain TNAU) and one biocontrol agent (Pseudomonas fluorescens strain PF1) for further studies in the pot culture. (bvsalud.org)
Bacterial2
- We recently reported that bacteriophage present at sites of bacterial infection are internalized by mammalian cells and that they trigger anti-viral immune responses that antagonize anti-bacterial responses. (stanford.edu)
- Four kinds of bacteriophage ( φ RSL, φ RSA, φ RSM and φ RSS) were isolated from Ralstonia solanacearum , a soil-borne Gram-negative bacterium that is the causative agent of bacterial wilt in many important crops. (microbiologyresearch.org)
Protein3
- A search for sequences related to known pseudomonad promoters suggests that the promoters in this bacteriophage may well be ntr-dependent, with the two strongest preceding the gene for the major coat protein (gene 8) and another ORF (430). (nih.gov)
- As proof of principle, the bacteriophages Pf3, Pf1, and M13 were engineered in this way to successfully synthesize Enhanced Green Fluorescent Protein (EGFP). (vcu.edu)
- Anodic aluminum oxide substrates with macroscopically aligned homogeneous nanopores of 80 nm in diameter enable two-dimensional, solid-state nuclear magnetic resonance studies of lipid-induced conformational changes of uniformly 15 N-labeled Pf1 coat protein in native-like bilayers. (infona.pl)
Viruses1
- Although early aggressive and prolonged treatment with specific antibiotics can extend survival in patients with cystic fibrosis (CF) colonized by opportunistic Pseudomonas aeruginosa (PA), antibiotics fail to eradicate the infecting multidrug-resistant (MDR) PA strains in CF. Century-long research has suggested treating patients with bacteriophages (phages, prokaryotic viruses) naturally hosted by bacteria. (frontiersin.org)
Virions1
- The calculation is based on the measurements of Day and Wiseman [Day, L.A. and Wiseman, R.L.: A comparison of DNA packaging in the virions of fd, Xf, and Pf1. (abdesignlabs.com)
Bacteria2
- The use of lytic bacteriophages has been proposed as an alternative treatment for infections caused by antibiotic-resistant bacteria. (edu.au)
- Disclosed methods include utilization of recombinant bacteriophage to deliver to pathogenic bacteria a translatable genetic sequence encoding an optically detectable marker or an enzyme capable of producing an optically detectable marker. (patentsencyclopedia.com)
Escherichia1
- Effects of bacteriophage fd infection on Escherichia coli HB11 envelope: a morphological and biochemical study. (semanticscholar.org)
Infection1
- Bacteriophage therapy for refractory Pseudomonas aeruginosa urinary tract infection. (semanticscholar.org)
Aeruginosa5
- Challenges and Promises for Planning Future Clinical Research Into Bacteriophage Therapy Against Pseudomonas aeruginosa in Cystic Fibrosis. (frontiersin.org)
- Bacteriophages for the treatment of Pseudomonas aeruginosa infections. (semanticscholar.org)
- Addition of F-actin, DNA or Pf1 bacteriophage to 0.5% agar plates increased swarming motility of Pseudomonas aeruginosa Xen5. (biomedcentral.com)
- This thesis examines the suitability of anti-P. aeruginosa lytic bacteriophages as a treatment for P. aeruginosa infections in CRS and CF-associated CRS. (edu.au)
- An in vitro study of the efficacy of a mixture of anti-P. aeruginosa bacteriophages (referred to as CT-PA) against a panel of 40 clinical P. aeruginosa respiratory isolates, from CRS and CF patients across 3 continents, was performed. (edu.au)
Lung infections1
- We have identified novel roles for bacteriophage (the 'Phageome') in the pathogenesis of wound and lung infections. (stanford.edu)
Studies1
- Sergeyev, Ivan V. & McDermott, Ann E. (2014) Studies of Water Populations in Pf1 Bacteriophage by NMR. (columbia.edu)
Method5
- 2. The method according to claim 1, wherein the recombinant bacteriophage is held within a delivery vehicle during the step of locating the recombinant bacteriophage in the in vivo environment. (patentsencyclopedia.com)
- 5. The method according to claim 2, further comprising degrading the delivery vehicle and releasing the recombinant bacteriophage from the delivery vehicle. (patentsencyclopedia.com)
- 6. The method according to claim 1, wherein the recombinant bacteriophage is lysin deficient. (patentsencyclopedia.com)
- 10. The method according to claim 9, wherein the bacteriophage carries genetic material encoding the cofactor. (patentsencyclopedia.com)
- 14. The method according to claim 1, further comprising locating the recombinant bacteriophage in the in vivo environment in conjunction with a medical device. (patentsencyclopedia.com)
Culture1
- and preparing a crude bacteriophage extract from the culture medium. (patentsencyclopedia.com)