DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Bacteriophage phi 6
Bacteriophage phi X 174
Databases, Nucleic Acid
Influenza A Virus, H3N8 Subtype
Influenza A virus
The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.
Myxovirus Resistance Proteins
Influenza A Virus, H1N1 Subtype
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
RNA Polymerase II
Journal Impact Factor
N4 RNA polymerase II, a heterodimeric RNA polymerase with homology to the single-subunit family of RNA polymerases. (1/14)Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3. These genes code for proteins of 269 and 404 amino acid residues with sequence similarity to the single-subunit, phage-like RNA polymerases. The genes encoding the N4 RNAPII subunits, as well as a synthetic construct encoding a fusion polypeptide, have been cloned and expressed. Both the individually expressed subunits and the fusion polypeptide reconstitute functional enzymes in vivo and in vitro. (+info)
The phage N4 virion RNA polymerase catalytic domain is related to single-subunit RNA polymerases. (2/14)In vitro, bacteriophage N4 virion RNA polymerase (vRNAP) recognizes in vivo sites of transcription initiation on single-stranded templates. N4 vRNAP promoters are comprised of a hairpin structure and conserved sequences. Here, we show that vRNAP consists of a single 3500 amino acid polypeptide, and we define and characterize a transcriptionally active 1106 amino acid domain (mini-vRNAP). Biochemical and genetic characterization of this domain indicates that, despite its peculiar promoter specificity and lack of extensive sequence similarity to other DNA-dependent RNA polymerases, mini-vRNAP is related to the family of T7-like RNA polymerases. (+info)
Escherichia coli single-stranded DNA-binding protein mediates template recycling during transcription by bacteriophage N4 virion RNA polymerase. (3/14)Coliphage N4 virion RNA polymerase (vRNAP), the most distantly related member of the T7-like family of RNA polymerases, is responsible for transcription of the early genes of the linear double-stranded DNA phage genome. Escherichia coli single-stranded DNA-binding protein (EcoSSB) is required for N4 early transcription in vivo, as well as for in vitro transcription on super-coiled DNA templates containing vRNAP promoters. In contrast to other DNA-dependent RNA polymerases, vRNAP initiates transcription on single-stranded, promoter-containing templates with in vivo specificity; however, the RNA product is not displaced, thus limiting template usage to one round. We show that EcoSSB activates vRNAP transcription at limiting single-stranded template concentrations through template recycling. EcoSSB binds to the template and to the nascent transcript and prevents the formation of a transcriptionally inert RNA:DNA hybrid. Using C-terminally truncated EcoSSB mutant proteins, human mitochondrial SSB (Hsmt SSB), phage P1 SSB, and F episome-encoded SSB, as well as a Hsmt-EcoSSB chimera, we have mapped a determinant of template recycling to the C-terminal amino acids of EcoSSB. T7 RNAP contains an amino-terminal domain responsible for binding the RNA product as it exits from the enzyme. No sequence similarity to this domain exists in vRNAP. Hereby, we propose a unique role for EcoSSB: It functionally substitutes in N4 vRNAP for the N-terminal domain of T7 RNAP responsible for RNA binding. (+info)
Phage N4 RNA polymerase II recruitment to DNA by a single-stranded DNA-binding protein. (4/14)Transcription of bacteriophage N4 middle genes is carried out by a phage-coded, heterodimeric RNA polymerase (N4 RNAPII), which belongs to the family of T7-like RNA polymerases. In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-stranded templates and low activity on single-stranded templates. In vivo, at least one additional N4-coded protein (p17) is required for N4 middle transcription. We show that N4 ORF2 encodes p17 (gp2). Characterization of purified gp2revealed that it is a single-stranded DNA-binding protein that activates N4 RNAPII transcription on single-stranded DNA templates through specific interaction with N4 RNAPII. On the basis of the properties of the proteins involved in N4 RNAPII transcription and of middle promoters, we propose a model for N4 RNAPII promoter recognition, in which gp2plays two roles, stabilization of a single-stranded region at the promoter and recruitment of N4 RNAPII through gp2-N4 RNAPII interactions. Furthermore, we discuss our results in the context of transcription initiation by mitochondrial RNA polymerases. (+info)
Bacteriophage N4 virion RNA polymerase interaction with its promoter DNA hairpin. (5/14)Bacteriophage N4 minivirion RNA polymerase (mini-vRNAP), the RNA polymerase (RNAP) domain of vRNAP, is a member of the T7-like RNAP family. Mini-vRNAP recognizes promoters that comprise conserved sequences and a 3-base loop-5-base pair (bp) stem DNA hairpin structure on single-stranded templates. Here, we defined the DNA structural and sequence requirements for mini-vRNAP promoter recognition. Mini-vRNAP binds a 20-nucleotide (nt) N4 P2 promoter deoxyoligonucleotide with high affinity (K(d) = 2 nM) to form a salt-resistant complex. We show that mini-vRNAP interacts specifically with the central base of the hairpin loop (-11G) and a base at the stem (-8G) and that the guanine 6-keto and 7-imino groups at both positions are essential for binding and complex salt resistance. The major determinant (-11G), which must be presented to mini-vRNAP in the context of a hairpin loop, appears to interact with mini-vRNAP Trp-129. This interaction requires template single-strandedness at positions -2 and -1. Contacts with the promoter are disrupted when the RNA product becomes 11-12 nt long. This detailed description of vRNAP interaction with its promoter hairpin provides insights into RNAP-promoter interactions and explains how the injected vRNAP, which is present in one or two copies, recognizes its promoters on a single copy of the injected genome. (+info)
X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase. (6/14)(+info)
Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. (7/14)(+info)
The tail sheath of bacteriophage N4 interacts with the Escherichia coli receptor. (8/14)(+info)
Phage - definition of phage by The Free Dictionary
Define phage. phage synonyms, phage pronunciation, phage translation, English dictionary definition of phage. n. A bacteriophage. n short for bacteriophage n. bacteriophage. a combining form meaning
A scalable pipeline for highly effective genetic modification of a malaria parasite<...
TY - JOUR. T1 - A scalable pipeline for highly effective genetic modification of a malaria parasite. AU - Pfander, Claudia. AU - Anar, Burcu. AU - Schwach, Frank. AU - Otto, Thomas D.. AU - Brochet, Mathieu. AU - Volkmann, Katrin. AU - Quail, Michael A.. AU - Pain, Arnab. AU - Rosen, Barry. AU - Skarnes, William. AU - Rayner, Julian C.. AU - Billker, Oliver. N1 - KAUST Repository Item: Exported on 2020-10-01. PY - 2011/10/23. Y1 - 2011/10/23. N2 - In malaria parasites, the systematic experimental validation of drug and vaccine targets by reverse genetics is constrained by the inefficiency of homologous recombination and by the difficulty of manipulating adenine and thymine (A+T)-rich DNA of most Plasmodium species in Escherichia coli. We overcame these roadblocks by creating a high-integrity library of Plasmodium berghei genomic DNA (,77% A+T content) in a bacteriophage N15-based vector that can be modified efficiently using the lambda Red method of recombineering. We built a pipeline for ...
Reactome | Pre-snRNA transcript initiation, Integrator binding, LEC binding
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
5121 Telomeres, centromeres, and other highly repetitive genomic regions are typically under-represented in shotgun libraries, and they are often absent from partial-digest BAC libraries. Accurate cloning of isolated fragments containing short tandem repeats, AT-rich DNA, or regions with strong secondary structure likewise can be difficult or impossible using conventional supercoiled plasmids. We have developed vectors and methods to alleviate these types of cloning bias. A transcription-free, linear plasmid was derived from the linear coliphage N15 for cloning into E.coli. The ends of the vector are free to rotate during replication, minimizing formation of secondary structures that are substrates for deletion or rearrangement. The pJAZZ linear vectors are shown to provide unprecedented ability to maintain regions of up to 30 kb that are unclonable in circular plasmids. Examples include regions of di-, tri-, and tetra-nucleotide repeats up to several kb, as well as highly AT-rich fragments of ...
Hatfull, G.F. and Sarkis, G.J. (1993). DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics. Mol. Microbiol., 7, 395-405.. Hatfull, G.F., Cresawn, S.G. and Hendrix, R.W. (2008). Comparative genomics of the mycobacteriophages: insights into bacteriophage evolution Res. Microbiol., 159, 332-339.. Lubbers, M.W., Waterfield, N.R., Beresford, T.P.J., Le Page, R.W.F. and Jarvis, A.W. (1995). Sequencing and analysis of the prolate-headed lactococcal bacteriophage c2 genome and identification of the structural genes. Appl. Environ. Microbiol., 61, 4348-4356.. Ravin, V., Ravin, N., Casjens, S., Ford, M., Hatfull, G. and Hendrix, R. (2000). Genomic sequence and analysis of the atypical temperate bacteriophage N15. J. Mol. Biol., 299, 53-73.. Robert, M.D., Martin, N.L. and Kropinski, A.M. (2004). The genome and proteome of coliphage T1. Virology, 318, 245-266.. Schouler, C., Ehrlich, S.D. and Chopin, M.-C. (1994). Sequence and organization of the ...
Name: Hydrogenophaga Willems et al. 1989. Category: Genus. Proposed as: gen. nov.. Etymology: Hy.dro.ge.no.pha.ga. N.L. neut. n. hydrogenum, hydrogen (that which produces water, so called because it forms water when exposed to oxygen); from Gr. neut. n. hydôr, water; from Gr. v. gennaô, to produce; Gr. v. phageîn, to eat, to devour; N.L. fem. n. Hydrogenophaga, eater of hydrogen Gender: feminine Type species: Hydrogenophaga flava (Niklewski 1910) Willems et al. 1989 Conduct genome-based taxonomy of genus at TYGS ...
N15likevirus - Wikipedia
N15likevirus (synonym N15-like viruses) is a genus of viruses in the order Caudovirales, in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There is currently only one species in this genus: the type species Enterobacteria phage N15. Group: dsDNA Order: Caudovirales Family: Siphoviridae Genus: N15likevirus Enterobacteria phage N15 N15likeviruses are nonenveloped, with a head and tail. The head is about 60 nm in diameter. The tail is long and flexible, at about 140 nm long, 8 nm wide, with short brush-like terminal fibers. Enterobacteria phage N15 has been fully sequenced. It has about 46k nucleotides, with 60 proteins. The complete genome is available here Viral replication is cytoplasmic. The virus attaches to the host cells adhesion receptors using its terminal fiber, and ejects the viral DNA into the host cytoplasm via long flexible tail ejection system. Replication follows the replicative transposition model. DNA-templated ...
Branch Detailed Info | Queens Library
In 1869, New York attorney Albon Platt Man purchased the Lefferts and Wellings Farms in West Jamaica, an area settled before the Revolutionary War. Envisioning a garden spot and refuge from city life in Manhattan, he recruited landscape architect Edward Richmond to help lay out his proposed community. It was one of the citys first planned garden communities. All streets were well laid out with trees planted on both sides. When Edward Richmond died in 1870, real estate developer, Oliver Fowler, became Mans partner. Lefferts Avenue, now Lefferts Boulevard, became the main thoroughfare.. Man called this new community Richmond Hill. Richmond Hill was also the name given to the 138 foot hill located north of Metropolitan Avenue on 116th Street. The name is believed to come from the London suburb of Richmond Hill, although some sources claim that it was named after Edward Richmond, the developer. Kew Gardens, originally North Richmond Hill, was established later in 1912. It was named after the ...
PAFAH2 - Wikipedia
Platelet-activating factor acetylhydrolase 2, cytoplasmic is an enzyme that in humans is encoded by the PAFAH2 gene. It is one of several PAF acetylhydrolases. This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. GRCh38: Ensembl release 89: ENSG00000158006 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000037366 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Hattori K, Adachi H, Matsuzawa A, Yamamoto K, Tsujimoto M, Aoki J, Hattori M, Arai H, Inoue K ...
Azorhizobium caulinodans nfrA protein Summary Report | CureHunter
Azorhizobium caulinodans nfrA protein: RNA-binding protein that stimulates the elongation of poly(A) tails; may be involved in the regulation of nifA; homologous to HF-I protein of Escherichia coli; Do Not confuse with nrfA, a cytochrome c nitrite reductase (EC 126.96.36.199); DO NOT confuse with NfrA protein of E. coli, a bacteriophage N4 adsorption protein; amino acid sequence given in first source; GenBank X76450
A novel intermediate in transcription initiation by human mitochondrial RNA polymerase
The mitochondrial genome is transcribed by a single-subunit T7 phage-like RNA polymerase (mtRNAP), structurally unrelated to cellular RNAPs. In higher eukaryotes, mtRNAP requires two transcription factors for efficient initiation-TFAM, a major nucleoid protein, and TFB2M, a transient component of mt …
Nucleotidyl | definition of nucleotidyl by Medical dictionary
Looking for online definition of nucleotidyl in the Medical Dictionary? nucleotidyl explanation free. What is nucleotidyl? Meaning of nucleotidyl medical term. What does nucleotidyl mean?
... the Structure of Bacteriophage N4". Journal of Molecular Biology. 378 (3): 726-36. doi:10.1016/j.jmb.2008.02.059. PMC 2396777. ... There is currently only one species in this genus: the type species Escherichia virus N4. Escherichia virus N4 is the type ... Enquatrovirus is a genus of bacteriophages in the order Caudovirales, in the family Podoviridae. Bacteria serve as natural ... Enquatrovirus Escherichia virus N4 The virus's virion have icosahedral (T=9) heads ~70 nm and short tails (10 nm), and contain ...
List of MeSH codes (B04)
... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ... bacteriophage n4 MeSH B04.280.090.700.070 - bacteriophage p22 MeSH B04.280.090.700.100 - bacteriophage t3 MeSH B04.280.090.700. ... bacteriophage m13 MeSH B04.123.205.260 - bacteriophage mu MeSH B04.123.205.280 - bacteriophage n4 MeSH B04.123.205.300 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
N4 phage P1 phage P2 phage P4 phage R17 phage T2 phage T4 phage (169 kbp genome, 200 nm long) T7 phage T12 phage Viruses portal ... coli bacteria Phage.org general information on bacteriophages bacteriophages illustrations and genomics Bacteriophages get a ... The largest bacteriophage genomes reach a size of 735 kb. Bacteriophage genomes can be highly mosaic, i.e. the genome of many ... Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found ...
Hershey AD, Chase M (May 1952). "Independent functions of viral protein and nucleic acid in growth of bacteriophage". The ... Adenosine N6-carbamoyl-methyladenine N6-methyadenine Modified Guanine 7-Deazaguanine 7-Methylguanine Modified Cytosine N4- ... The reason for the presence of these noncanonical bases in bacterial viruses (bacteriophages) is to avoid the restriction ...
Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found ... A bacteriophage (/bækˈtɪərioʊfeɪdʒ/), also known informally as a phage (/feɪdʒ/), is a virus that infects and replicates within ... 2×108 bacteriophages per mL. Bacteriophages are thought to extensively contribute to horizontal gene transfer in natural ... Main article: Marine bacteriophage. Metagenomics has allowed the in-water detection of bacteriophages that was not possible ...
... was achieved in 1974 in the laboratory of Charles Weissmann using a nucleotide analogue N4- ... Hutchison Ca, 3.; Edgell, M. H. (1971). "Genetic Assay for Small Fragments of Bacteriophage φX174 Deoxyribonucleic Acid". ... effect of an extracistronic mutation on the in vitro propagation of bacteriophage Qbeta RNA". Proc Natl Acad Sci U S A. 72 (1 ... The ssUDNA is extracted from the bacteriophage that is released into the medium, and then used as template for mutagenesis. An ...
List of restriction enzyme cutting sites: Ba-Bc
Huang LH, Farnet CM, Ehrlich KC, Ehrlich M (March 1982). "Digestion of highly modified bacteriophage DNA by restriction ... "Cytosine modification in DNA by BcnI methylase yields N4-methylcytosine". FEBS Lett. 161 (1): 131-4. doi:10.1016/0014-5793(83) ...
Mutagenesis (molecular biology technique)
Such chemicals include aminopurine, which induces an AT to GC transition, while nitrosoguanidine, bisulfite, and N4- ... Favor AH, Llanos CD, Youngblut MD, Bardales JA (2020). "Optimizing bacteriophage engineering through an accelerated evolution ... effect of an extracistronic mutation on the in vitro propagation of bacteriophage Qbeta RNA". Proceedings of the National ...
Viruses | Free Full-Text | Comparative Analysis of 37 Acinetobacter Bacteriophages | HTML
3.3.2. Acinetobacter Phage Presley Is an N4-Like Singleton. A single N4-like Acinetobacter phage, Presley, has been described [ ... Abedon, S.T. Bacterial immunity against bacteriophages. Bacteriophage 2012, 2, 50-54. [Google Scholar] [CrossRef] [PubMed] ... like N4 and the N4-like phages of Pseudomonas aeruginosa LUZ7, LIT1 and PEV2, the giant virion RNAP of Presley is devoid of ... Comparative Analysis of 37 Acinetobacter Bacteriophages. by Dann Turner 1,*, Hans-Wolfgang Ackermann 2,†, Andrew M. Kropinski 3 ...
Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption<...
Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption. D. R. Kiino, M. S. Singer, L. B. ... Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption. / Kiino, D. R.; Singer, M. S.; ... Kiino, DR, Singer, MS & Rothman-Denes, LB 1993, Two overlapping genes encoding membrane proteins required for bacteriophage N4 ... We present the nucleotide sequences of two genes whose products are required for bacteriophage N4 adsorption. The nfrA gene ...
Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption - Fingerprint - University of...
Background Bacteriophage EC1-UPM can be an N4-like bacteriophage which infects O78:K80 - BET-bromodomain inhibition research
Background Bacteriophage EC1-UPM can be an N4-like bacteriophage which infects O78:K80. Posted on July 25, 2017. by ... Background Bacteriophage EC1-UPM can be an N4-like bacteriophage which infects O78:K80 specifically, an avian pathogenic stress ... In this study, we report for the first time the genome of a bacteriophage EC1-UPM, an N4-like bacteriophage that infects O78: ... Bacteriophage N4 which infects K-12 is currently the most studied strain for this group of bacteriophages . In addition to ...
Isolation and characterization of a N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves
... ... Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non- ... A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture ... In addition to the very large virion encapsulated DNA-dependent RNA polymerase which is the signature of the N4-like genus, an ...
The complete genome sequence of EC1-UPM, a novel N4-like bacteriophage that infects Escherichia coli O78:K80 | Virology Journal...
Leveraging on the availability of multiple N4-like bacteriophage genome sequences, the core genes of N4-like bacteriophages ... Comparative genomics analysis reveals close resemblance of bacteriophage EC1-UPM to three N4-like bacteriophages namely vB_EcoP ... We report for the first time the complete genome sequence of a N4-like bacteriophage which is lytic against avian pathogenic ... The complete genome sequence of bacteriophage EC1-UPM was analysed and compared with other closely related N4-like phage groups ...
Enquatrovirus - Wikipedia
... the Structure of Bacteriophage N4". Journal of Molecular Biology. 378 (3): 726-36. doi:10.1016/j.jmb.2008.02.059. PMC 2396777. ... There is currently only one species in this genus: the type species Escherichia virus N4. Escherichia virus N4 is the type ... Enquatrovirus is a genus of bacteriophages in the order Caudovirales, in the family Podoviridae. Bacteria serve as natural ... Enquatrovirus Escherichia virus N4 The viruss virion have icosahedral (T=9) heads ~70 nm and short tails (10 nm), and contain ...
Viruses | Free Full-Text | Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Frontiers | Two New Lytic Bacteriophages of the Myoviridae Family Against Carbapenem-Resistant Acinetobacter baumannii |...
The isolation of two new lytic bacteriophages in this study could expand our sight on Acinetobacter bacteriophages and may ... For most lytic bacteriophages, a set of holin and endolysin are required for lysis. However, no known holin-encoding genes were ... For most lytic bacteriophages, a set of holin and endolysin are required for lysis. However, no known holin-encoding genes were ... No tRNAs but 88 and 89 open reading frames (ORFs) were predicted for the two bacteriophages, among which 22 and 21 had known ...
VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression ofvps...
1993) A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption. J. Bacteriol. 175:7074-7080. ... which is required for bacteriophage N4 adsorption and encodes UDP-N-acetylglucosamine 2-epimerase function (19). It is also ... The ligation mixture was packaged into bacteriophage lambda particles using the Gigapack III gold packaging extract (Stratagene ...
Visualization of uncorrelated, tandem symmetry mismatches in the internal genome packaging apparatus of bacteriophage T7 | PNAS
2008) Insight into DNA and protein transport in double-stranded DNA viruses: The structure of bacteriophage N4. J Mol Biol 378( ... Inside some bacteriophages, which include T7 (19, 21, 22), P-SSP7 (11), and ε15 (17), a roughly cylindrical, multilayered ... 1983) Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol 166(4):477-535. ... 2011) Peering down the barrel of a bacteriophage portal: The genome packaging and release valve in p22. Structure 19(4):496-502 ...
Frontiers | Emerging methods to study bacteriophage infection at the single-cell level | Microbiology
2010). Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses". Virology ... Emerging methods to study bacteriophage infection at the single-cell level. Vinh T. Dang1 and Matthew B. Sullivan1,2* ... Davis, B. M., Kimsey, H. H., Chang, W., and Waldor, M. K. (1999). The Vibrio cholerae O139 Calcutta bacteriophage CTXφ is ... Hitch, G., Pratten, J., and Taylor, P. W. (2004). Isolation of bacteriophages from the oral cavity. Lett. Appl. Microbiol. 39, ...
Nearly Identical Bacteriophage Structural Gene Sequences Are Widely Distributed in both Marine and Freshwater Environments |...
41), clusters N1 to N4 were assigned by Wang et al. (35), and clusters 1 to 6 were assigned by Dorigo et al. (5). We assigned ... Effects of bacteriophages on the population dynamics of four strains of pelagic marine bacteria. Microb. Ecol. 42:395-406. ... Nearly Identical Bacteriophage Structural Gene Sequences Are Widely Distributed in both Marine and Freshwater Environments. ... Evolutionary relationships among diverse bacteriophages and prophages: all the worlds a phage. Proc. Natl. Acad. Sci. USA 96: ...
Table of Contents | Journal of Virology
Complete Genome Sequence of IME11, a New N4-Like Bacteriophage Huahao Fan, Hang Fan, Xiaoping An, Yong Huang, Zhiyi Zhang, ... Complete Genome Sequence of Bp7, an Escherichia coli Bacteriophage with a Wide Host Range Can Zhang, Wenhua Liu, Huiying Ren ... Complete Genome Sequence of T4-Like Escherichia coli Bacteriophage HX01 Fang Tang, Yanzhe Li, Wei Zhang, Chengping Lu ... Complete Genome Sequence of Cronobacter sakazakii Bacteriophage vB_CsaM_GAP161 Reza Abbasifar, Andrew M. Kropinski, Parviz M. ...
RNA Polymerase II | Journal of Bacteriology
List of MeSH codes (B04) - Wikipedia
... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ... bacteriophage n4 MeSH B04.280.090.700.070 - bacteriophage p22 MeSH B04.280.090.700.100 - bacteriophage t3 MeSH B04.280.090.700. ... bacteriophage m13 MeSH B04.123.205.260 - bacteriophage mu MeSH B04.123.205.280 - bacteriophage n4 MeSH B04.123.205.300 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 | Virology...
Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and ... Rothman-Denes : Enterobacteria Phage N4 (Podoviridae). Encyclopedia of Virology 2004, 450-454. full_textGoogle Scholar. ... Hyman ST: Bacteriophage Host Range and Bacterial Resistance. Adv Appl Microbiol 2010, 70: 217-248. full_textPubMedView Article ... Song HY, Xu XM, Zhang : Isolation of a novel polyvalent virulent bacteriophage of E. coli. J Med Coll PLA 2007, 22: 261-267. ...
Azorhizobium caulinodans nfrA protein Summary Report | CureHunter
KEGG SSDB Best Search Result: hsa:29079
IIT Bombay Research Internship Awards - India Education Diary
Phylogenetic tree based on the virion RNA polymerase (a | Open-i
... of N4-like bacteriophages for which genome sequences are available. Th ... f5: Phylogenetic tree based on the virion RNA polymerase (a) and large terminase subunits (b) of N4-like bacteriophages for ... f5: Phylogenetic tree based on the virion RNA polymerase (a) and large terminase subunits (b) of N4-like bacteriophages for ... We isolated phiAxp-3, an N4-like bacteriophage that infects A. xylosoxidans, from hospital waste and studied its genomic and ...
Independent Transport and Sorting of Functionally Distinct Protein Families in Tetrahymena thermophila Dense Core Secretory...
Complete genomic sequence of the Vibrio alginolyticus bacteriophage Vp670 and characterization of the lysis-related genes, cwlQ...
Biocontrol of bacterial pathogens by bacteriophages (phages) represents a promising strategy. Vibrio alginolyticus, a gram- ... Li B, Zhang S, Long L, Huang S. Characterization and complete genome sequences of three N4-like roseobacter phages isolated ... Measurement of the rate of attachment of bacteriophage to cells. In: Clokie, MRJ, Kropinski AM, editors. Bacteriophages: ... Young R. Bacteriophage lysis: mechanism and regulation. Microbiol Rev. 1992;56(3):430-81.PubMedPubMed CentralGoogle Scholar. ...
Dr. Satheesh Babu T. G. | Amrita Vishwa Vidyapeetham
In this study, we report the isolation and characterization of a novel P. aeruginosa N4-like lytic bacteriophage, vB_Pae_AM.P2 ... "A Novel N4-Like Bacteriophage Isolated from a Wastewater Source in South India with Activity against Several Multidrug- ... Genome sequencing of AM.P2 revealed features similar to those of the N4-like P. aeruginosa phages LUZ7 and KPP21. As judged by ... Thus, AM.P2 may represent an intriguing candidate for inclusion in bacteriophage cocktails developed for various applications, ...
Publication | Nilai University
Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway
Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages [PeerJ]
Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. In ... Comparative analysis of the BM5 and BM10 genome structures, in conjunction with other B. megaterium bacteriophages, revealed ... megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. Growth analyses indicated that the ... technologies provide unique possibilities for the comprehensive assessment of the environmental diversity of bacteriophages. ...
Bacteriophage Orphan DNA Methyltransferases: Insights from Their Bacterial Origin, Function, and Occurrence | Applied and...
N-4-methylcytosine (class II), or C-5-methylcytosine (class III). Often, these MTases are associated with a cognate restriction ... His research interests focus on bacteriophage-host interactions, including the host response to bacteriophage infection and ... Cleavage of the bacteriophage P1 packaging site (pac) is regulated by adenine methylation. Proc. Natl. Acad. Sci. U. S. A. 87: ... The genome of bacteriophage K1F, a T7-like phage that has acquired the ability to replicate on K1 strains of Escherichia coli. ...
MacCHESS User Publications | CHESS
Basu R. S., Murakami K. S. Watching the bacteriophage N4 RNA polymerase transcription by time-dependent soak-trigger-freeze X- ... Roy A., Bhardwaj A. and Cingolani G. Crystallization of the nonameric small terminase subunit of bacteriophage P22. Acta ... Molodtsov, V., and Murakami, K.S. "Minimalism and functionality: Structural lessons from the heterodimeric N4 bacteriophageRNA ...
SMART: Secondary literature for GARS C domain
We found that the n4 locus, and especially the nodM and nodN genes, are involved in the production of the root hair deformation ... The GART domain was expressed in E. coli under the control of bacteriophage T7 promotor and isolated by a three-step ... The nucleotide sequence of the nod box locus n4 in Rhizobium meliloti was determined and revealed six genes organized in a ... The sequences restricted only by McrB+C+ include T-even bacteriophage containing 5-hydroxymethylcytosine (restriction of this ...
PhagesVirusesInfectsBacterialEscherichiaStructure of Bacteriophage N4Lytic bacteriophageGenesBacteriaCharacterizationSequencesPodoviridaeGenusPseudomonasEnterobacteriaLysisAcinetobacterProteinsColiPhylogeneticGenomic sequencePolymeraseSynthesisPodovirusInfectionBacillusEndolysinCaudoviralesGeneticPathogenicRecombinantSewageComparativeIsolatesDiversityMorphologyVRNAPProcapsidTailStaphylococcalPhage therapyNucleic acidsSequence1988TranscriptionMolecularGenomesBaumannii
- Multilocus phylogenetic evaluation using primary genes of sequenced N4-like phages demonstrated how the evolutionary romantic relationship correlated well using the design of sponsor specificity. (healthandwellnesssource.org)
- Recently, a number of genetically related phages were isolated, infecting Silicibacter and Sulfitobacter (DSS3ɸ2 and EE36ɸ1) as well as a number of Pseudomonas phages (LUZ7, LIT1 and PEV2) Group: dsDNA Order: Caudovirales Family: Podoviridae Genus: Enquatrovirus Escherichia virus N4 The virus's virion have icosahedral (T=9) heads ~70 nm and short tails (10 nm), and contain short fibers originating from the junction between the head and tail. (wikipedia.org)
- Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. (biomedcentral.com)
- Genome sequence comparisons place phiAxp-3 more closely with JWAlpha and JWDelta Achromobacter phages than with other N4 viruses. (nih.gov)
- Biocontrol of bacterial pathogens by bacteriophages (phages) represents a promising strategy. (biomedcentral.com)
- Bacteriophages (phages) belong to a category of viruses that infect and lyse bacteria and are natural and abundant in the marine environment. (biomedcentral.com)
- The bacteriophages life cycle has two stages: a lytic stage where the phages reproduce inside the bacteria and lyse bacteria and a lysogenic stage where the phage is in a stationary stage where do not exist phage reproduction. (revistabionatura.com)
- The bacteriophages are an alternative to fight against the antimicrobial or pesticides because phages offer advantages such as high host specificity, the ability of long term effect, are active against dividing or not dividing bacterial cells, effective elimination of biofilms and are capable vehicles for nucleic acids delivery. (revistabionatura.com)
- In the following review, in the main topics in bacteriophages and its applications in agriculture: the bacteriophages life cycle, advantages of phages in biological control applications, the last isolated phages and described for different pathogens and the last advances in phage engineering applications for biological control. (revistabionatura.com)
- N4-like phages are podoviruses distributed into four genera frequently isolated from different Gram-negative species and characterized by the presence of a giant virion-associated RNA polymerase [34,35,36]. (chromoscience.com)
- However, the tail fiber proteins of RiverRider are clearly distinct from those of other N4-like phages. (bvsalud.org)
- Bacteriophage in the genus T7-like Phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7. (medconditions.net)
- The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. (bvsalud.org)
- First genome sequences of Achromobacter phages reveal new members of the N4 family. (uni-bielefeld.de)
- Wittmann J, Dreiseikelmann B, Rohde M, Meier-Kolthoff JP, Bunk B, Rohde C. First genome sequences of Achromobacter phages reveal new members of the N4 family. (phagoflow.de)
- Bacteriophages, or phages for short, are viruses that infect bacteria, take over the molecular machinery inside the bacterial cells and use it to make more copies of themselves. (elifesciences.org)
- The genome encoded 83 hypothetical proteins, among which a giant protein of 3398 amino acids (ORF66), the characteristic virion-encapsulated RNA polymerases of N4-like viruses (Fig 6)  which allows transcription of early genes in these phages. (nih.gov)
- Ab09, like other N4-like phages encoded a second type of RNA polymerase (ORF18 and ORF19), a heterodimeric T7-like RNAP. (nih.gov)
- Bacterial viruses (bacteriophages) are considered to be the most prevalent entities in the biosphere [ 1 ]. (mdpi.com)
- The tailed dsDNA bacteriophages and human dsDNA viruses, such as herpesviruses and adenoviruses, share many features of structural organization and life cycle. (pnas.org)
- Phylogenetic analysis of vRNAP from different N4 viruses revealed that phiAxp-3, JWAlpha, and JWDelta formed a separate clade from the other N4-like viruses (Fig. 5a). (nih.gov)
-  Bacteriophages are ubiquitous viruses, found wherever bacteria exist. (wikipedia.org)
- A species in the genus N4-like viruses, in the family PODOVIRIDAE, that infects E. coli. (bvsalud.org)
- 2008). Bacteriophages (phage), the viruses of bacteria, have received increased research interest in recent years as an environmentally friendly means of controlling bacterial diseases. (cit.ie)
- A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis. (medconditions.net)
- To unravel the influence of viruses, and particularly bacteriophages, on microbiota ecology and animal host physiology and health, methods allowing quantitative comparison of virus diversity, abundance and function across samples are needed. (biomedcentral.com)
- Accepting the view that R/M systems have evolved to defend bacteria effectively against attack by bacterial viruses, the chapter discusses the question of what mechanisms bacteriophages have developed to overcome barriers provided by host R/M systems. (asmscience.org)
- Background Bacteriophage EC1-UPM can be an N4-like bacteriophage which infects O78:K80 specifically, an avian pathogenic stress that triggers colibacillosis in chicken. (healthandwellnesssource.org)
- In this study, we report for the first time the genome of a bacteriophage EC1-UPM, an N4-like bacteriophage that infects O78:K80. (healthandwellnesssource.org)
- Bacteriophage N4 which infects K-12 is currently the most studied strain for this group of bacteriophages . (healthandwellnesssource.org)
- Escherichia virus N4 is the type species of this genus and was originally isolated from sewers in Genoa, Italy and infects Escherichia coli K-12. (wikipedia.org)
- We isolated phiAxp-3, an N4-like bacteriophage that infects A. xylosoxidans, from hospital waste and studied its genomic and biological properties. (nih.gov)
- The complete genome sequence and genetic analysis of ØEC1, a novel N4-like bacteriophage that infects Escherichia coli O78:K80. (nilai.edu.my)
- A bacteriophage ( / b æ k ˈ t ɪər i oʊ f eɪ dʒ / ), also known informally as a phage ( / f eɪ dʒ / ), is a virus that infects and replicates within Bacteria and Archaea . (wikipedia.org)
- Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. (medconditions.net)
- Bacteriophages and bacterial plant diseases. (cit.ie)
- The bacterial life cycle of the bacteriophages there exists two phases: a lytic and a lysogenic stage. (revistabionatura.com)
- Bacteriophages play an important role in shaping the bacterial genome by exerting a selective pressure . (chromoscience.com)
- Bacterial resistance to bacteriophage is principally mediated by modification of the phage receptors although other mechanisms can play a role by blocking the viral entry and multiplication . (chromoscience.com)
- 2. The method according to claim 1, wherein the concentration of said antibiotic in the medium is in the range which causes about 1% to about 99% inhibition of the growth of said bacterial strain in the absence of said bacteriophage. (patentsencyclopedia.com)
- CONCLUSION: Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. (bvsalud.org)
- The interactions of bacteriophages and their hosts, analyzed according to the categories of bacterial R/M systems and the mechanisms that bacteriophages have evolved to overcome restriction, represent an interesting case of coevolution of two competing organisms. (asmscience.org)
- The bacteriophages then break open, or "lyse", the bacterial cell, releasing the viral copies into the environment, ready to infect more bacteria nearby. (elifesciences.org)
- Hays and Seed saw that the lidI gene disrupts lysis inhibition, speeding up the bursting of infected bacterial cells, which in turn decreases the number of bacteriophages produced from each infected cell. (elifesciences.org)
Structure of Bacteriophage N41
- B. Structure of bacteriophage N4 determined by cryo-EM and 3D image reconstruction techniques. (utmb.edu)
- The current presence of lysis gene however, not lysogeny-related gene shows that bacteriophage EC1-UPM can be a lytic bacteriophage. (healthandwellnesssource.org)
- A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. (biomedcentral.com)
- Previously, we isolated a multi-drug resistant D. tsuruhatensis strain ARB-1 from a municipal wastewater treatment plant along with the lytic bacteriophage. (biomedcentral.com)
- The lytic bacteriophage RG-2014 belongs to the Podoviridae family in the order Caudovirales . (biomedcentral.com)
- We present the nucleotide sequences of two genes whose products are required for bacteriophage N4 adsorption. (elsevier.com)
- Leveraging over the option of multiple N4-like Rabbit Polyclonal to NPY5R bacteriophage genome buy Panipenem sequences, the primary genes of N4-like bacteriophages had been identified and utilized to execute a multilocus phylogenetic evaluation which allowed the construction of the phylogenetic tree with higher self-confidence than phylogenetic trees and shrubs based on one genes. (healthandwellnesssource.org)
- To help expand exploit the potential of bacteriophage EC1-UPM, it is essential to have an understanding on its genetic make-up, particularly the genes which are responsible for the infection and lysis of the host bacteria. (healthandwellnesssource.org)
- The absence of genes related to lysogeny along with the high efficacy observed during in vitro cell lysis trials, indicate that the vB_VspP_pVa5 is a potential candidate component in a bacteriophage cocktail suitable for the biological control of V. splendidus in aquaculture. (dtu.dk)
- Phage N4 employs at least three genes encoding RNAPs for the transcription of genes in different stages of its life cycle23. (nih.gov)
- In the N4 genome the RNAP1 gene is followed directly by RNAP2, but in the phiAxp-3 genome insertions of two small genes (ORF15 and ORF16) occur between RNAP1 and RNAP2. (nih.gov)
- Occurrence of the increased number sequences bacteriophage genomes and biocomputer science allows to predict with some degree of reliability of function of some phage genes. (bio.su)
- This gene is part of a system that defends against bacteriophage attack called the PLE, which consists of several genes of previously unknown function. (elifesciences.org)
- The comparative analysis of bacteriophage genome sequences has greatly enhanced our understanding of their diversity, revealing relationships between phage genomes often infecting distantly related host bacteria. (mdpi.com)
- The host specificity of N4-like bacteriophages is rather diverse, ranging from enterobacteria such as and to marine bacteria such as sp. (healthandwellnesssource.org)
- It is estimated there are more than 10 31 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. (wikipedia.org)
- The bacteriophages use different strategies to attack bacteria by introducing their DNA into the bacterias or by producing lysins. (revistabionatura.com)
- During the lytic stage, the bacteriophages introduce their nucleic acid into the bacteria, by the action of an enzymatic mechanism for penetrating the peptidoglycan layer and inner membrane and ensure the release the phage DNA. (revistabionatura.com)
- Bacteria and their bacteriophages coexist and coevolve for the benefit of both in a mutualistic association. (chromoscience.com)
- It binds with nucleic acids and inactivates both bacteria and bacteriophage. (nih.gov)
- Disclosed methods include utilization of recombinant bacteriophage to deliver to pathogenic bacteria a translatable genetic sequence encoding an optically detectable marker or an enzyme capable of producing an optically detectable marker. (patentsencyclopedia.com)
- Bacteriophages: A Therapy Concept against Multi-Drug-Resistant Bacteria. (nih.gov)
- Rohde C, Wittmann J, Kutter E. Bacteriophages: A Therapy Concept against Multi-Drug-Resistant Bacteria. (phagoflow.de)
- Bacteria, bacteriophages that prey upon them, and mobile genetic elements (MGEs) compete in dynamic environments, evolving strategies to sense the milieu. (elifesciences.org)
- Temperate bacteriophage Phi O18P from an Aeromonas media isolate: Characterization and complete genome sequence. (uni-bielefeld.de)
- The cl repressor of bacteriophage P1: isolation and characterization of the repressor protein. (uni-bielefeld.de)
- Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus. (usp.br)
- Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya. (phagoflow.de)
- Dreiseikelmann B, Velleman M, Schuster H. The cl repressor of bacteriophage P1: isolation and characterization of the repressor protein. (uni-bielefeld.de)
- Phylogenetic tree based on the virion RNA polymerase (a) and large terminase subunits (b) of N4-like bacteriophages for which genome sequences are available. (nih.gov)
- In this study, we describe the biological characteristics, whole genome sequences, and annotations for two new isolates of the B. megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. (peerj.com)
- To confirm the hypothesis that the high frequency sequences of high throughput sequencing are the terminal sequences of the bacteriophage genome. (bvsalud.org)
- Enquatrovirus is a genus of bacteriophages in the order Caudovirales, in the family Podoviridae. (wikipedia.org)
- Transmission electron microscopy revealed that, with a 67-nm diameter icosahedral head and a 20-nm non-contractile tail, phiAxp-3 has features characteristic of Podoviridae bacteriophages (order Caudovirales). (nih.gov)
- Whole genomic sequencing revealed 45.4- or 45.8-kb genome with a 37.6% GC content for WCHABP1 and WCHABP12, both of which showed significant DNA sequence similarity with bacteriophages of the Ap22virus genus within the Myoviridae family. (frontiersin.org)
- We analyse here the mutations emerging in a clinical Pseudomonas aeruginosa strain in response to infection by Ab09, a N4-like lytic podovirus and describe a variety of chromosomal deletions and mutations conferring resistance. (chromoscience.com)
- Bacteriophage 8P was isolated with a Pseudomonas stutzeri strain isolated from an oil reservoir as its host bacterium. (bvsalud.org)
- Bacteriophage PEV20 and Ciprofloxacin Combination Treatment Enhances Removal of Pseudomonas aeruginosa Biofilm Isolated from Cystic Fibrosis and Wound Patients. (nih.gov)
- Investigation of a Large Collection of Pseudomonas aeruginosa Bacteriophages Collected from a Single Environmental Source in Abidjan, Côte d'Ivoire. (nih.gov)
- Twenty two distinct bacteriophages were isolated from sewage water from five locations in the city of Abidjan, Côte d'Ivoire over a two-year period, using a collection of Pseudomonas aeruginosa strains with diverse genotypes. (nih.gov)
- Things are getting hairy: Enterobacteria bacteriophage vB_PcaM_CBB. (cit.ie)
- No tRNAs but 88 and 89 open reading frames (ORFs) were predicted for the two bacteriophages, among which 22 and 21 had known function and encoded proteins for morphogenesis, packaging, lysis, and nucleiotide metabolism. (frontiersin.org)
- For most lytic bacteriophages, a set of holin and endolysin are required for lysis. (frontiersin.org)
- The genome is characteristic of N4-like bacteriophages in both content and organization, having predicted proteins characterized into the functional groups of transcription, DNA metabolism, DNA replication, lysis, lysis inhibition, structure and DNA packaging. (bvsalud.org)
- The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains. (uni-bielefeld.de)
- Hays and Seed set out to understand how the timing of lysis can impact the bacteriophage, using the bacterium Vibrio cholerae - which causes cholera - and its bacteriophage called ICP1. (elifesciences.org)
- This "lysis inhibition" gives the bacteriophages more time to make copies of themselves inside the bacterium, so even more are released when the cell finally breaks open. (elifesciences.org)
- Now that researchers know that ICP1 bacteriophages also delay lysis in Vibrio cholerae , this might lead to more studies exploring this process in samples from cholera patients. (elifesciences.org)
- Two lytic bacteriophages, WCHABP1 and WCHABP12, were recovered from hospital sewage and were able to infect 9 and 12 out of 18 carbapenem-resistant Acinetobacter baumannii clinical strains, which belonged to different clones. (frontiersin.org)
- The isolation of two new lytic bacteriophages in this study could expand our sight on Acinetobacter bacteriophages and may offer new potential therapeutic alternatives against A. baumannii . (frontiersin.org)
- In addition to having its complete genome buy Panipenem sequenced, the identity and locations of several of its structural proteins have been established through comparisons of three-dimensional, cryo-electron microscopic structures of wild-type N4 and its mutants . (healthandwellnesssource.org)
- The C-terminal amino acids of the large unit of fiber tail proteins varied between the bacteriophages, which may explain their different host ranges. (frontiersin.org)
- Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome , and may have relatively simple or elaborate structures. (wikipedia.org)
- In collaboration with Dr. Lucia Rothman-Denes laboratory (University of Chicago), we are determining the structure of N4 virions, including their tail component proteins. (utmb.edu)
- Amino acid sequence comparisons for proteins in these categories showed highest similarities to well-characterized N4-like bacteriophages isolated from Achromobacter xylosoxidans and Erwinia amylovora. (bvsalud.org)
- The function of the cps19fK product was confirmed by its ability to complement a mutation in nfrC (rffE) in E. coli, as judged by restoration of sensitivity to bacteriophage N4. (nih.gov)
- Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. coli phage N4 indicating that it is a member of the N4-like phage group. (biomedcentral.com)
- The interactions between E. coli and phage N4, and the basis for phage-resistance have been largely studied but little is known about the other members of this family. (chromoscience.com)
- Elucidation of the evolutionary relationship of various N4-like bacteriophages based on multilocus and single-gene phylogenetic analyses. (biomedcentral.com)
- Similar high throughput sequencing technique was applied to analyze the genomic sequence of N4-like bacteriophage IME11. (bvsalud.org)
- The current presence of bacteriophage-encoded RNA polymerase of such size can be a personal of N4 bacteriophage. (healthandwellnesssource.org)
- N4 packages dsDNA as well as one or two copies of a 3,500-amino acid RNA polymerase inside the capsid, which is then ejected into host cell upon infection. (utmb.edu)
- The efficiency of translesion synthesis across 3,N4-ethenocytosine and 1, N6-ethenoadenine is around 20%, a value that is similar to the in vivo efficiency deduced from the effect of the lesions on the survival of transfected M13 ssDNA. (nih.gov)
- Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. (dtu.dk)
- Inside our earlier research, bacteriophage EC1-UPM that was isolated from poultry faecal sample can reduce the intensity of infection due to O78:K80 and gets the potential to be utilized for the treating colibacillosis in hens. (healthandwellnesssource.org)
- To test the efficacy of the bacteriophages in protecting against A. baumannii infection, a Galleria mellonella larva model was used. (frontiersin.org)
- Bacteriophage that infect Roseobacters ('roseophage') have the potential to shunt secondary production into the dissolved carbon pool and through the process of infection alter Roseobacter physiology. (tennessee.edu)
- Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. (peerj.com)
- Development of a tomato plant resistant to Clavibacter michiganensis using the endolysin gene of bacteriophage CMP1 as a transgene. (uni-bielefeld.de)
- RESULTS: Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order. (bvsalud.org)
- The genetic components of bacteriophage EC1-UPM which may be responsible for its host specificity and virulence were identified and analyzed family. (healthandwellnesssource.org)
- Tailed bacteriophages have to deliver their genetic information into the host cell's cytoplasm across cell membranes. (utmb.edu)
- Here, we describe three new Caulobacter bacteriophages (Jess A, SR18, and RW) that show genetic similarity to BPP-1 but have many different genetic and structural features that differentiate them from BPP-1. (bvsalud.org)
- 10. The method according to claim 9, wherein the bacteriophage carries genetic material encoding the cofactor. (patentsencyclopedia.com)
- Bottom line We survey for the very first time the entire genome sequence of the N4-like bacteriophage which can be lytic against avian pathogenic O78:K80. (healthandwellnesssource.org)
- 2. The method according to claim 1, wherein the recombinant bacteriophage is held within a delivery vehicle during the step of locating the recombinant bacteriophage in the in vivo environment. (patentsencyclopedia.com)
- 5. The method according to claim 2, further comprising degrading the delivery vehicle and releasing the recombinant bacteriophage from the delivery vehicle. (patentsencyclopedia.com)
- 6. The method according to claim 1, wherein the recombinant bacteriophage is lysin deficient. (patentsencyclopedia.com)
- 14. The method according to claim 1, further comprising locating the recombinant bacteriophage in the in vivo environment in conjunction with a medical device. (patentsencyclopedia.com)
- METHODS: Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. (bvsalud.org)
- Comparative analysis of the BM5 and BM10 genome structures, in conjunction with other B. megaterium bacteriophages, revealed relatively high levels of sequence and organizational identity. (peerj.com)
- Next-Generation Sequencing (NGS) technologies provide unique possibilities for the comprehensive assessment of the environmental diversity of bacteriophages. (peerj.com)
- This minireview will focus on bacteriophage-encoded MTases, their prevalence and diversity, as well as their potential origin and function. (asm.org)
- Electron microscopy scan showed that both bacteriophages had the similar morphology as those of the Myoviridae family. (frontiersin.org)
- vRNAP is packed into the capsid and is injected into the host cell together with phage DNA, which makes N4 the only known phage that does not depend on host RNAP for transcription of its early genes24. (nih.gov)
- We have used single-particle electron cryomicroscopy to study the multilayer structure of the portal vertex of the bacteriophage T7 procapsid, the recipient of T7 DNA in packaging. (pnas.org)
- Jet nebulization of bacteriophages with different tail morphologies - Structural effects. (nih.gov)
- That evidence allow to bacteriophage serves like a biological control in the crops and agriculture problems, also known as phage therapy, which is a central topic of the present review. (revistabionatura.com)
- Bacteriophage interactions with marine pathogeniuc vibrios: Implications for phage therapy (review). (proaqua.dk)
- In recent years, advances in sequencing technologies and data analysis techniques have enabled detailed metagenomic studies investigating intestinal viromes (collections of bacteriophage and eukaryotic viral nucleic acids) and their potential contributions to the ecology of the microbiota. (biomedcentral.com)
- Evidences were provided that N4-like bacteriophage had a particular terminal sequence: the left end of the genome was unique while the right end was permuted. (bvsalud.org)
- The high throughput sequencing technique was convenient and practical to be used to simultaneously detect the terminal sequence and the complete sequence of bacteriophage genome. (bvsalud.org)
- propiolactone and ethylenimines, in an example with MS2 bacteriophage" Thesis andtranslation (1988). (patentgenius.com)
- As an N4 like virus, phiAxp-3 also harbours three different RNAPs, suggesting the same transcription strategy as that used by N4. (nih.gov)
- Isolation and molecular characterisation of Achromobacter phage phiAxp-3, an N4-like bacteriophage. (nih.gov)
- Bacteriophages occur abundantly in the biosphere, with different genomes, and lifestyles. (wikipedia.org)
- 20% G. mellonella larvae survived at 96 h after being infected by carbapenem-resistant A. baumannii strains, from which the two bacteriophages were recovered. (frontiersin.org)