A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Viruses whose hosts are bacterial cells.
Viruses whose host is Escherichia coli.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.
Proteins found in any species of virus.
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
The functional hereditary units of VIRUSES.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.
Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.
A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.
The process by which a DNA molecule is duplicated.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Viruses whose nucleic acid is DNA.
An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.
Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7.
Any method used for determining the location of and relative distances between genes on a chromosome.
A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.
A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
The functional hereditary units of BACTERIA.
Proteins found in any species of bacterium.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Viruses whose host is Staphylococcus.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A family of bacteriophages which are characterized by short, non-contractile tails.
A class of opioid receptors recognized by its pharmacological profile. Mu opioid receptors bind, in decreasing order of affinity, endorphins, dynorphins, met-enkephalin, and leu-enkephalin. They have also been shown to be molecular receptors for morphine.
Viruses whose host is Streptococcus.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Proteins obtained from ESCHERICHIA COLI.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The sum of the weight of all the atoms in a molecule.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.

An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction. (1/284)

A highly efficient DNA sequencing strategy was developed on the basis of the bacteriophage Mu in vitro DNA transposition reaction. In the reaction, an artificial transposon with a chloramphenicol acetyltransferase (cat) gene as a selectable marker integrated into the target plasmid DNA containing a 10.3-kb mouse genomic insert to be sequenced. Bacterial clones carrying plasmids with the transposon insertions in different positions were produced by transforming transposition reaction products into Escherichia coli cells that were then selected on appropriate selection plates. Plasmids from individual clones were isolated and used as templates for DNA sequencing, each with two primers specific for the transposon sequence but reading the sequence into opposite directions, thus creating a minicontig. By combining the information from overlapping minicontigs, the sequence of the entire 10,288-bp region of mouse genome including six exons of mouse Kcc2 gene was obtained. The results indicated that the described methodology is extremely well suited for DNA sequencing projects in which considerable sequence information is on demand. In addition, massive DNA sequencing projects, including those of full genomes, are expected to benefit substantially from the Mu strategy.  (+info)

SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA). (2/284)

In bacteria, SsrA RNA recognizes ribosomes stalled on defective messages and acts as a tRNA and mRNA to mediate the addition of a short peptide tag to the C-terminus of the partially synthesized nascent polypeptide chain. The SsrA-tagged protein is then degraded by C-terminal-specific proteases. SmpB, a unique RNA-binding protein that is conserved throughout the bacterial kingdom, is shown here to be an essential component of the SsrA quality-control system. Deletion of the smpB gene in Escherichia coli results in the same phenotypes observed in ssrA-defective cells, including a variety of phage development defects and the failure to tag proteins translated from defective mRNAs. Purified SmpB binds specifically and with high affinity to SsrA RNA and is required for stable association of SsrA with ribosomes in vivo. Formation of an SmpB-SsrA complex appears to be critical in mediating SsrA activity after aminoacylation with alanine but prior to the transpeptidation reaction that couples this alanine to the nascent chain. SsrA RNA is present at wild-type levels in the smpB mutant arguing against a model of SsrA action that involves direct competition for transcription factors.  (+info)

Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. (3/284)

A bipartite enhancer sequence (composed of the O1 and O2 operator sites) is essential for assembly of the functional tetramer of phage Mu transposase (MuA) on supercoiled DNA substrates. A three-site interaction (LER) between the left (L) and right (R) ends of Mu (att sites) and the enhancer (E) precedes tetramer assembly. We have dissected the role of the enhancer in tetramer assembly by using two transposase proteins that have a common att site specificity, but are distinct in their enhancer specificity. The activity of these proteins on substrates containing hybrid enhancers reveals a 'criss-crossed' pattern of interaction between att and enhancer sites. The left operator, O1, of the enhancer interacts specifically with the transposase subunit at the R1 site (within the right att sequence) that is responsible for cleaving the left end of Mu. The right operator, O2, shows a preferential interaction with the transposase subunit at the L1 site (within the left att sequence) that is responsible for cleaving the right end of Mu.  (+info)

Natural synthesis of a DNA-binding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi. (4/284)

We have identified a 34 kDa DNA-binding protein with an HU-like activity in the Lyme disease spirochete Borrelia burgdorferi. The 34 kDa protein is translated from an abundant transcript initiated within the gene encoding the A subunit of DNA gyrase. Translation of the 34 kDa protein starts at residue 499 of GyrA and proceeds in the same reading frame as full-length GyrA, resulting in an N-terminal-truncated protein. The 34 kDa GyrA C-terminal domain, although not homologous, substitutes for HU in the formation of the Type 1 complex in Mu transposition, and complements an HU-deficient strain of Escherichia coli. This is the first example of constitutive expression of two gene products in the same open reading frame from a single gene in a prokaryotic cellular system.  (+info)

Replacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication. (5/284)

The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108 allowed efficient Mu replication and was shown to contain a strong gyrase site. However, neither the strong gyrase site from the plasmid pSC101 nor the major gyrase site from pBR322 could promote efficient Mu replication, even though the pSC101 site is a stronger gyrase site than the Mu SGS as assayed by cleavage in the presence of gyrase and the quinolone enoxacin. To look for SGS-like sites in the Escherichia coli chromosome which might be involved in organizing nucleoid structure, fragments of E. coli chromosomal DNA were substituted for the SGS: first, repeat sequences associated with gyrase binding (bacterial interspersed mosaic elements), and, second, random fragments of the entire chromosome. No fragments were found that could replace the SGS in promoting efficient Mu replication. These results demonstrate that the gyrase sites from the transposing phages possess unusual properties and emphasize the need to determine the basis of these properties.  (+info)

Organization and dynamics of the Mu transpososome: recombination by communication between two active sites. (6/284)

Movement of transposable genetic elements requires the cleavage of each end of the element genome and the subsequent joining of these cleaved ends to a new target DNA site. During Mu transposition, these reactions are catalyzed by a tetramer of four identical transposase subunits bound to the paired Mu DNA ends. To elucidate the organization of active sites within this tetramer, the subunit providing the essential active site DDE residues for each cleavage and joining reaction was determined. We demonstrate that recombination of the two Mu DNA ends is catalyzed by two active sites, where one active site promotes both cleavage and joining of one Mu DNA end. This active site uses all three DDE residues from the subunit bound to the transposase binding site proximal to the cleavage site on the other Mu DNA end (catalysis in trans). In addition, we uncover evidence that the catalytic activity of these two active sites is coupled such that the coordinated joining of both Mu DNA ends is favored during recombination. On the basis of these results, we propose that the DNA joining stage requires a cooperative transition within the transposase-DNA complex. The cooperative utilization of active sites supplied in trans by Mu transposase provides an example of how mobile elements can ensure concomitant recombination of distant DNA sites.  (+info)

Identification of SoxS-regulated genes in Salmonella enterica serovar typhimurium. (7/284)

Salmonella enterica serovar Typhimurium responds to superoxide-generating agents through soxR-mediated activation of the soxS gene, whose product, SoxS, is necessary for resistance to oxidative stress. The S. enterica serovar Typhimurium soxRS system also mediates redox-inducible resistance to diverse antibiotics, which may be relevant to clinical infections. In order to identify SoxS-regulated genes in S. enterica serovar Typhimurium, a lacI-regulated expression system for the S. enterica serovar Typhimurium soxS gene was developed. This system was used to demonstrate that soxS expression is sufficient for the induction of resistance to the superoxide-generating drug paraquat and for the transcriptional activation of the sodA and micF genes. In addition, a library of random lacZ insertions was generated and screened for clones displaying differential beta-galactosidase activity in the presence or absence of SoxS. This selection yielded six independent chromosomal lacZ transcriptional fusions that were activated by either artificial expression of SoxS or exposure of wild-type cells to micromolar concentrations of paraquat. Moreover, disruption of the inducible genes by the insertions rendered S. enterica serovar Typhimurium hypersensitive to millimolar concentrations of paraquat. Nucleotide sequence determination identified the disrupted genes as sodA (Mn-containing superoxide dismutase), fpr (NADPH:ferredoxin oxidoreductase), and ydbK (a putative Fe-S-containing reductase).  (+info)

Mu DNA reintegration upon excision: evidence for a possible involvement of nucleoid folding. (8/284)

Mutations induced by the integration of a Mugem2ts prophage can revert at frequencies around 1x10(-6). In these revertant clones, the prophage excised from its original localization is not lost but reintegrated elsewhere in the host genome. One of the most intriguing aspects of this process is that the prophage reintegration is not randomly distributed: there is a strong correlation between the original site of insertion (the donor site) and the target site of the phage DNA migration (the receptor site). In this paper, it is shown that in the excision-reintegration process mediated by Mugem2ts, the position of the initial prophage site strongly influences the location of the reintegration site. In addition, for each donor site, the receptor site is a discrete DNA region within which the excised Mu DNA can reintegrate and the two sites implicated in phage DNA migration must be located on the same DNA molecule. These data suggest the involvement of nucleoid folding in the excision-reintegration process.  (+info)

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Transposition allows movement of a defined stretch of DNA, a transposon, from one DNA location to another. This process is required for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic resistances between bacterial populations; and it is responsible for spontaneous mutations in all the kingdoms of life. Transposition is mediated by a protein, the transposase, encoded by the transposon. DNA sequence signals at the two ends of the transposon activate assembly of a transpososome: a complex that include multiple copies of transposase plus both transposon ends. Transpososome assembly, in turn, activates the DNA cleavage and joining reactions required for transposition. This thesis explores aspects of interactions between one transposase, MuA, and the ends of its transposon DNA, the genome of bacteriophage Mu. The first chapter provides an overview of Mu transposition, with special emphasis on the transpososome. The second chapter shows that in the absence of ...
1BCM: Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.
E. coli ClpX is a member of the Clp/Hsp100 family of ATPases that remodel multi-component complexes and facilitate ATP-dependent protein degradation. Protein remodelers alter the biological activity of their substrates, typically by changing the quaternary structure of their target proteins. ClpX remodels protein-DNA complexes, termed transpososomes, made during recombination of the phage Mu. When recombination is complete, the core four-subunit transpososome complex does not spontaneously release the DNA; transposase remains so stably bound that subsequent replication of the Mu genome is inhibited. To understand how ClpX releases the replication block without destroying the transpososomes, we characterized the mechanism and products of transpososome remodeling. To better understand the mechanism ClpX uses to facilitate remodeling, I first participated in a collaborative project that defined major biochemical reaction steps involved in protein degradation by ClpX and its associated peptidase ...
Background: Tangle analysis has been applied successfully to study proteins which bind two segments of DNA and can knot and link circular DNA. We show how tangle analysis can be extended to model any stable protein-DNA complex. -- Results: We discuss a computational method for finding the topological conformation of DNA bound within a protein complex. We use an elementary invariant from knot theory called colorability to encode and search for possible DNA conformations. We apply this method to analyze the experimental results of Pathania, Jayaram, and Harshey (Cell 2002). We show that the only topological DNA conformation bound by Mu transposase which is biologically likely is the five crossing solution found by Pathania et al (although other possibilities are discussed). -- Conclusion: Our algorithm can be used to analyze the results of the experimental technique described in Pathania et al in order to determine the topological conformation of DNA bound within a stable protein-DNA complex ...
Let $\mathcal{B}_0$ denote the cylinder $\sigma$-algebra. Since a cylinder set $A \in \mathcal{B}_0$ only specifies the values of functions at countably many points, if it is nonempty then it contains a discontinuous function. Hence the inner measure of $C([0,1])$ is $$\mu_*(C([0,1])) = \sup\{\mu(A) : A \in \mathcal{B}_0, A \subset C([0,1])\} = \sup\{\mu(\emptyset)\} = 0.$$ Since $\mu_*(C([0,1])) \ne \mu^*(C([0,1]))$ it is not $\mu$-measurable and is not in the $\mu$-completion of $\mathcal{B}_0$.. ...
Families with certain genetic mutations face many complex and personal choices, including how and when to share such information with their children. Moms Genes is here to help.
Le poper in morda nageljnove žbice imata med začimbami tako bogato in krvavo zgodovino kot muškat. Zdravnikom v Antiki je bil muškat neznan. »Sadovi« muškata so prispeli v Evropo verjetno s križarskimi vitezi. Prvi zanesljivi vir je bizantinski zdravnik Simon Seth, ki je v 10. stoletju napisal o muškatu »da koristi želodcu, jetrom in srcu«, vendar je že tedaj svaril pred pretiranim uživanjem. Najprej so ga po morju in karavanskih poteh prinašali v Evropo arabski trgovci. V tem času je stalo pol kilograma muškata toliko kot tri ovce. V 16. stoletju so ga imenovali »Zlato Vzhodne Indije«. Angleži, Španci, Portugalci in Nizozemci so se krvavo bojevali za sadove muškatnega drevesa. Kot rezultat teh bojev so Angleži zamenjali otok Run v vzhodnoindijskem arhipelagu za veliko večji otok na vhodni obali severne Amerike, ki je bil v nizozemski lasti. Ta otok je Manhattan in je imel tedaj manj kot 1000 prebivalcev. Trgovina z muškatom je prinašala ogromne dobičke. Na otokih je ...
Le poper in morda nageljnove žbice imata med začimbami tako bogato in krvavo zgodovino kot muškat. Zdravnikom v Antiki je bil muškat neznan. »Sadovi« muškata so prispeli v Evropo verjetno s križarskimi vitezi. Prvi zanesljivi vir je bizantinski zdravnik Simon Seth, ki je v 10. stoletju napisal o muškatu »da koristi želodcu, jetrom in srcu«, vendar je že tedaj svaril pred pretiranim uživanjem. Najprej so ga po morju in karavanskih poteh prinašali v Evropo arabski trgovci. V tem času je stalo pol kilograma muškata toliko kot tri ovce. V 16. stoletju so ga imenovali »Zlato Vzhodne Indije«. Angleži, Španci, Portugalci in Nizozemci so se krvavo bojevali za sadove muškatnega drevesa. Kot rezultat teh bojev so Angleži zamenjali otok Run v vzhodnoindijskem arhipelagu za veliko večji otok na vhodni obali severne Amerike, ki je bil v nizozemski lasti. Ta otok je Mahattan in je imel tedaj manj kot 1000 prebivalcev. Trgovina z muškatom je prinašala ogromne dobičke. Na otokih je ...
Using the Tukey method for each of the six comparisons yields: $$ \begin{eqnarray} 0.29 \le & \mu_2 - \mu_1 & \le 4.47 \\ & & \\ 1.13 \le & \mu_3 - \mu_1 & \le 5.31 \\ & & \\ -2.25 \le & \mu_1 - \mu_4 & \le 1.93 \\ & & \\ -2.93 \le & \mu_2 - \mu_3 & \le 1.25 \\ & & \\ 0.13 \le & \mu_2 - \mu_4 & \le 4.31 \\ & & \\ 0.97 \le & \mu_3 - \mu_4 & \le 5.15 \\ \end{eqnarray ...
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CEACAM 5 + 6小鼠单克隆抗体[MUS](ab4539)可与人样本反应并经WB, ELISA, IHC, Flow Cyt实验严格验证,被4篇文献引用。所有产品均提供质保服务,中国75%以上现货。
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பகல் பன்னிரண்டு மணிக்கு மீண்டும் திருச்சூரை நோக்கிப் புறப்பட்டோம். திருச்சூரைக் கடந்து குன்னங்குளம் என்ற ஊரை நோக்கி எங்கள் மகிழ்வுந்து பறந்தது. நல்ல சாலை அமைப்பு என்பதால் திட்டமிட்டதை விட வேகமாக நாங்கள் திருச்சூரைக் கடந்தோம். வழியில் அண்ணன் வீரக்குமரன் அவர்களின் விரிவுரை மகிழ்வுந்தில் அமைந்தது. முனைவர் வீரக்குமரன் அவர்கள் தமிழ், ஆங்கிலம், மலையாளம் என ...
Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The contributions from the secondary structure of the transcriptional activator protein C of bacteriophage Mu to its specific DNA binding and the influence of various factors, viz ., electrolytes, and minor groove and major groove binders on this protein-DNA interaction have been addressed . Circular dichroism (CD) spectral results suggest that, in the absence of Mg2+, C protein exhibits a j3-pleated sheetlike structure and Mg2+ changes the conformation to a more a-helical structure which could provide specific geometrical constraints complementary to those of DNA helix. Thus, Mg 2+ acts as a cofactor for the binding of the C protein to its specific site in DNA by inducing conformational changes in the protein. Competitive binding studies with minor and major groove binding drugs, viz ., distamycin A and methyl green, respectively, and the DMS footprinting data indicate that the C protein recognizes the major groove of DNA during complex formation . Further, upon major groove binding, C protein ...
Hsmar1, a cut-and-paste element of the mariner transposon family, was recently investigated to examine the kinetics of the complex formation pathway. This transposase forms a dimer in solution before binding to first one transposon end, then recruiting the other. This pathway, Synapsis by Naked End Capture (S-NEC), is found in both prokaryotes and eukaryotes. Tn5, of the IS4 family, is an example of the alternative pathway, Synapsis by Protein Dimerisation (S-PD). In this pathway, the transposase is monomeric in solution. Each monomer binds to a transposon end independently, before combining to form a synaptic complex. This pathway has been seen widely in prokaryotes, but no eukaryotic elements tested to date employ it ...
Like AZT, 1NP also inhibited 4MU glucuronidation by UGT2B7 without a substantial effect on substrate cooperativity (at least at low 1NP concentrations). However, inhibition resulted mainly from a decrease in Vmax. The model used to describe these data again assumes binding of inhibitor at a distinct effector site but with abolition of product formation from enzyme complexes containing inhibitor. As is evident from Fig. 7, A and C, rate of product formation approaches zero at high modifier concentration. Although experimental data were well described by the three-site model, the increase in S50 and decrease in Hill coefficient observed at the highest modifier concentrations suggests that 1NP may also act as a competitive inhibitor of 4MU glucuronidation.. In contrast to the inhibition of 4MU glucuronidation by 1NP, 4MU activated the formation of 1NPG by UGT2B7. Kinetic data were well described by the three-site model illustrated in Fig. 2B, where modifier binds at an effector site that is ...
Having professional family photos taken can be an ordeal, but its basically a necessary one to endure. Because at the end of the day, who doesnt love having a perfect-looking family portrait to hang on the wall?. Unless youre a member of the Zaring family, that is. Because their recent family photos are going viral for being hilariously awful.. Pam Zaring is a mom who just wanted some nice family pictures like any mom does. So she sought out the services of a professional photographer in her area, paid $250 for said services, and gathered her entire family and two dogs together for some scenic photos on a warm, sunny day.. The results? OMG. You just have to see them for yourself. You. Will. Die.. LOL forever. When family pictures go wrong, usually its because a kid is crying or someones eyes are closed. In this case, its the, uh, interesting editing choices.. Please see these FOR REAL photos she delivered to us, Zaring wrote in the post. She said the shadows were really bad on the ...
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
Tzv. INSPIRE prioritní data jsou data, která spadají do některého z témat INSPIRE (příloha III) a zároveň jsou to data, kterými je naplňován reporting v oblasti životního prostředí. Jedná se o tematické oblasti: Voda, Kvalita ovzduší & Hluk, Příroda & Biodiverzita, Opady a Průmysl (Mořské oblasti nejsou pro ČR relevantní). Pro tato prioritní data musí být zpřístupněna metadata tak, aby je bylo možné přímo zobrazit a stáhnout ...
Question - what is the best way for the treatment for mu tumer and who - 5Y. Find the answer to this and other Neurology questions on JustAnswer
TY - JOUR. T1 - (+)-CC-1065 as a structural probe of Mu transposase-induced bending of DNA. T2 - Overcoming limitations of hydroxyl-radical footprinting. AU - Ding, Zhi Ming. AU - Harshey, Rasika M.. AU - Hurley, Laurence. PY - 1993/9/11. Y1 - 1993/9/11. N2 - Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90°. To probe the directionality and locus of these A-protein-induced bends, we have used the antitumor antibiotic (+)-CC-1065 as a structural probe. As a consequence of binding within the minor groove, (+)-CC-1065 is able to alkylate N3 of adenine in a sequence selective manner. This selectivity is partially determined by conformational flexibility of the DNA sequence, and the covalent adduct has a bent DNA structure in ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Abrahams, P. J., Mulder, C., Vandevoorde, A., Warnaar, S. O., Vandereb, A. J. (1975) Transformation of Primary Rat-Kidney Cells by Fragments of Simian Virus 40 DNA. Journal of Virology, 16 (4). pp. 818-823. Allet, Bernard, Bukhari, Ahmad I. (March 1975) Analysis of Bacteriophage Mu and Lambda-Mu Hybrid Dnas by Specific Endonucleases. Journal of Molecular Biology, 92 (4). pp. 529-536. Apte, B. N., Rhodes, H., Zipser, D. (September 1975) Mutation blocking the specific degradation of reinitiation polypeptides in E. coli. Nature, 257 (5524). pp. 329-31. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of Terminal Repetition in Adenovirus 2 DNA. Cold Spring Harbor Symposia on Quantitative Biology, 39. pp. 401-407. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of terminal repetition in adenovirus 2 DNA. Cold Spring Harb Symp Quant Biol, 39 Pt . pp. 401-7. Atkins, J. F., Gesteland, R. F. (August 1975) The synthetase gene of the RNA phages R17, MS2 and f2 has a single UAG terminator ...
Indicatii: Eliminarea parazitilor intestinali in cure de 7 zile repetate dupa o pauza de alte 7 zile pentru prevenirea recidivelor. Femelele măsoară până la zece milimetri în lungime și ajung să efectueze ouatul spre rect, unde cu mușcăturile lor provoacă prurit enervant pe marginea anusului. Apasă pentru a vedea definiția originală «oxiuro» în dicționarul Spaniolă dictionary.
Konsolidovaný počiatočný finančný výkaz Eurosystému k 1. januáru 2011a konsolidovaný finančný výkaz Eurosystému k 7. januáru 2011
Argentina - Servidor de Mu Online dedicado Multilenguaje Internacional en Español .:Mu Online:. MuOnline Server 1.04D - Season 6 EP3 - Ranking Elfas Top Reset Master - Argentina
Prečo je pre mužov/ženy análny styk zaujímavý (Tesnejšie zovretie? Veľa ľudí chce svoj sex v posteli okoreniť, siahajú preto po erotických pomôckach. Análny sex. Pokiaľ nie ste vopred dohodnutí, nepchaj sa tam, kde nemáš.
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MU Online Season 6 Private Server Medium x1000 - x100 Dynamic. Added new custom boxes, boses, events, items, 4 level wings and a lot of other attractions
Diabetik je človek trpiaci cukrovkou a jeho život sa chorobe musí prispôsobiť. Pokiaľ sa diabetik riadi radami lekárov a dodržuje liečbu, môže prežuť plnohodnotný život.
Vo víkendovej Veľkej cene Singapuru formuly 1 najviac zaujala havária z úvodného kola, po ktorej skončilo viacero pretekárov.
Busy Moms is a community for Moms on the move, here you will find helpful information for working and stay-at-home Moms. We hope you enjoy ...
Kuba wahinga byumwuga ngo ntabwo bisaba kuba warabyize cyangwa ufite amafaranga menshi cyane nkuko bisobanurwa numuhinzi twasuye kugira ngo adusobanurire ibyo guhinga no korora kijyambere mu Rwanda. Emmanuel Ntuyenabo wize ubwubatsi ubu ni umuhinzi mworozi wabigize umwuga kandi amaze kugera kuri byinshi mu myaka itandatu abimazemo. Yatangije miliyoni umunani, ubu umutungo we wikubye inshuro nyinshi …. Uko wakura ubukire mu mwuga wubuhinzi nubworozi Read More ». ...
To say gin is having a moment would underestimate its popularity, and they can be as varied as they are delicious: fruity, floral, spicy or herbal. Whatever your preference, theres a gin for you - but tonic is another story. Find out more about how tonic is made and how to match it to your gin.
To say gin is having a moment would underestimate its popularity, and they can be as varied as they are delicious: fruity, floral, spicy or herbal. Whatever your preference, theres a gin for you - but tonic is another story. Find out more about how tonic is made and how to match it to your gin.
Mikroalbuminurie zvyšuje u diabetiků výrazně nejen riziko vývoje diabetické nefropatie, ale i kardiovaskulárních komplikací. Zbývající část článku naleznete v přiloženém PDF.
Există în corp şi unele zone fără bacterii în mod normal: muşchii, sângele şi sistemul nervos. Bacterii autotrofe fotosintetizante care folosesc energia solară pentru producerea de substanţe organice.
+Analysis andIsolation ofPlasminogenActivator fromMammalian CellCulture BrothDelaine Zayas-Bazán BurgosJohn Muñoz TorresVibha Bansal PhDMr. Javier RosadoMr. Ca…
Researchers define turning points as a major transformation in views about the self, identity or the meaning of life. They occur as new things are learned, rendering us amenable to change, and produce perceived, long-lasting redirection in the path of a ones life. Psychologists associate turning points with transitions and stages of human development defined and explored by Erik Erikson. Ignoring uplifting turning points and with distressing turning points in mind, the philosopher Frederick Nietzsche wrote that which does not kill us makes us stronger. Retirement or loss of retirement income, end of a love affair, reaching the golden years (maturity) or learning that one (or a family member) has a fatal disease are examples of turning points. Portrayals, in film and literature, of individuals coping with obstacles to happiness or overcoming adversity dramatize turning points. Rhetorical, films and literature are cultural artifacts that comfort, guide generations and teach us how to live! Lessons
TY - JOUR. T1 - Genomic DNA transposition induced by human PGBD5. AU - Henssen, Anton G.. AU - Henaff, Elizabeth. AU - Jiang, Eileen. AU - Eisenberg, Amy R.. AU - Carson, Julianne R.. AU - Villasante, Camila M.. AU - Ray, Mondira. AU - Still, Eric. AU - Burns, Melissa. AU - Gandara, Jorge. AU - Feschotte, Cedric. AU - Mason, Christopher E.. AU - Kentsis, Alex. N1 - Publisher Copyright: © Henssen et al.. PY - 2015/9/25. Y1 - 2015/9/25. N2 - Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its ...
Transposase is an enzyme that binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. The word transposase was first coined by the individuals who cloned the enzyme required for transposition of the Tn3 transposon. The existence of transposons was postulated in the late 1940s by Barbara McClintock, who was studying the inheritance of maize, but the actual molecular basis for transposition was described by later groups. McClintock discovered that pieces of the chromosomes changed their position, jumping from one chromosome to another. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed. Transposition in maize causes changes in color; however, in other organisms, such as bacteria, it can cause antibiotic resistance. Transposition is also important in creating genetic diversity within species and adaptability to ...
SCOTTSDALE, Ariz. - August marks a significant 10-year anniversary for patients with digestive problems. Just one decade ago, in August 2001, the FDA approved use of capsule endoscopy technology to examine the last frontier of the human GI tract - the small bowel.. Mayo Clinic in Arizona was among the first medical centers in the U.S. to perform exams of the small bowel using the pioneering pill camera technology that can scan the far corners of the GI tract - painlessly and without surgery.. Over the past decade, Mayo Clinic as a three-site organization (Arizona, Florida and Minnesota) has completed more than 7,000 such exams, where patients swallow a vitamin-sized capsule that contains a miniature camera. The capsule then travels through the digestive tract, snapping thousands of pictures that are transmitted to a recorder that patients wear around their waist for eight to 12 hours. Images saved on the recorder are transferred to a computer to create a video of the digestive tract, ...
The Community Foundation is participating in a three-site public art project that includes Big Spring Park, City Centre, and the new Community Foundation Park. Nestled in the heart of Huntsville, Community Foundation Park at the corner of Lowe and Pelham Avenues, adjacent to the downtown Redstone Federal Credit Union.. The featured art installation is made possible through the extraordinary generosity of Bob and Phylis Baron. An accompanying donor recognition wall is made possible by Community Foundation Founding Benefactors Joe and Pam Alexander. Together, these pieces create a beautiful park that will honor donors who are improving the quality of life in this place we all call home.. Community Foundation Park will recognize donors who have given at least $250,000 to or through the Community Foundation during their lifetimes, with a special distinction for philanthropic relationships at the $1,000,000 level and above.. Additionally, a designated area on the donor wall will honor Legacy Society ...
Zimwe mu nzara zizwi zabayeho, hari inzara ya Rukungugu yabaye ku ngoma yUmwami Yuhi IV Gahindiro. Iyo nzara yabaye ahagana mu ntangiriro zikinyejana cya XIX. Yatewe namapfa yiganje mu gihugu. Nkuko Padiri Alexis Kagame abyandika mu gitabo cye yise « Un Abrégé de lhistoire du Rwanda », Umwami Gahindiro nabiru bakoresheje icyo gihe Inzira yubwiru ya Rukungugu mu kuyirwanya. Iyo nzara yamaze umwaka umwe.. Nyuma yaho, mu gihe cyubukoloni bwAbadage, mu Rwanda habaye nizindi nzara, zirimo Ruyaga yo mu 1897-1903, Rwakabaga cyangwa se Kimwaramwara yo mu 1906-1909. Inzara Ruyaga yatewe ninzige zateye imyaka, zikayona kugeza aho bivuyemo inzara. Naho Inzara ya Rwakabaga yatewe namapfa yiganje cyane mu majyaruguru no mu burasirazuba bwigihugu.. Mu gihe cyubukoloni bwAbabiligi, u Rwanda rwatewe nizindi nzara zikomeye. Izo ni nka Rumanura cyangwa se Rumanurimbaba yo mu ntambara ya mbere yisi yose. Iyo nzara yabaye hagati yi 1916 ni 1918. Yatewe nintambara, ihunga ryabantu, ubusahuzi ...
displaystyle {\begin{aligned}{\mathcal {L}}_{\mathrm {QCD} }&={\bar {\psi }}_{i}\left(i\gamma ^{\mu }(D_{\mu })_{ij}-m\,\delta _{ij}\right)\psi _{j}-{\frac {1}{4}}G_{\mu \nu }^{a}G_{a}^{\mu \nu }\\&={\bar {\psi }}_{i}(i\gamma ^{\mu }\partial _{\mu }-m)\psi _{i}-gG_{\mu }^{a}{\bar {\psi }}_{i}\gamma ^{\mu }T_{ij}^{a}\psi _{j}-{\frac {1}{4}}G_{\mu \nu }^{a}G_{a}^{\mu \nu }\,,\\\end{aligned ...
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This paper derives (by a new method) an equation due to Macdonald for determining the zeros of the associated Legendre functions of order m and non-integral degree n when the argument is close to -1. A closed form solution is obtained for the values of Q subscript n superscript m (mu) and Q subscript n superscript -m (mu) for mu close to 1. Certain observations are made concerning errors in a recently published article.(*RADAR CROSS SECTIONS
Muži Sexy Otvorené Rozkroku Nit Čierny Faux PU Kožené Latex Šortky Erotické Boxerky Duté Vonku Šortky Muž Nohavičky Fetish Gay Club Nosenie
Ebbaasi ebadde ewenyuka obuweewo etomereganye ne loole mu bitundu bye Kyazanga Masaka, omuntu omu afiiriddewo nabalala abasoba mu 20 baddusiddwa amu ddwaaliro nga bataawa ...
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Dozens of cases of the Mu COVID-19 variant - recently declared a variant of interest - have been identified in B.C. According to the B.C. Centre for Disease Controls latest weekly update on . . .
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Bukeye umukobwa numuhungu, mu masaha yigicamunsi barahura ngo batembere, sinzi uko umuhungu yaje kurabukwa igishyimbo mu menyo yumukobwa, umuhungu aba uwo munsi yari afite twa duti dukura ibiryo mu menyo, amuha kamwe agira ati Akira ukure ifiriti mu menyo. ...
During the Fourth Eurosymposium on Healthy Ageing (EHA), we had the opportunity to meet Dr. Daniel Muñoz-Espín from the University of Cambridge.
12 Benefits of Breastfeeding for Both Mom and Baby - the benefits of breastfeeding for both mom and baby include - breastfeeding for both mom and baby
How many moms were...... - Just thought it would be intersting to see how many moms were high risk, why and how many week you were when you... -...
Mužský a ženský organizmus má svoje špecifiká. Súvisí to aj s evolúciou, v rámci ktorej sa u mužov vyvinula reakcia „útok alebo útek, ktorá znamenala „prežiť a zároveň slúžila ako výberový mechanizmus, kedy prežili iba tí najsilnejší jedinci. Všetko, čo potrebuje mužský organizmus, obsahuje FOR MAN:. ...
Today we we remind you of a few reasons why your mom is the best...just in time for Mothers Day! Here are 25 Reasons You Should Thank Your Mom!
Worried mom - Im new to this site, have many questions so please be patient. My daughter is 19 and is dating a 17 year old is this normal,...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Üniversitemiz bünyesinde dokuz fakülte, iki enstitü ve altı meslek yüksekokulu bulunmaktadır. MŞÜ, topluma ve yaklaşık 9000 öğrencisine, 580 akademik ve 391 idari personeli ile 166 derslik, 24 laboratuvar ve toplantı salonunda; merkezi laboratuvarları ve müstakil etkinlik alanlarında hizmet vermektedir.
Španiel Alberto Contador z tímu Tinkoff-Saxo sa vrátil v sobotňajšej dlhej časovke na 59,4 km z Trevisa do Valdobbiadene do ružového dresu vedúceho muža cyklistických pretekov Giro dItalia.. ...
... , also known as mu phage or mu bacteriophage, is a muvirus (the first of its kind to be identified) of the ... 2002), "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and ... created a crystal structure of the Mu bacteriophage transpososome, allowing for a detailed understanding of the process Mu ... Crystal Structure of the Bacteriophage MU Transpososome". Nature. 491: 413-417. doi:10.2210/pdb4fcy/pdb. Phage Mu at ViralZone ...
Mathee, K; Howe, M M (1993-09-01). "Bacteriophage Mu Mor protein requires sigma 70 to activate the Mu middle promoter". Journal ... Her early research identified the function and regulatory mechanisms of the bacteriophage Mu. Her postdoctoral research focused ... Function and regulation of the bacteriophage Mu middle operon (Thesis). OCLC 28138881. Kalai, Mathee; K.Fry, Norman (1 July ... Mathee, K; Howe, M M (1990-12-01). "Identification of a positive regulator of the Mu middle operon". Journal of Bacteriology. ...
Bacteriophages. Interscience, New York. OCLC 326505 Ho, N. B., Z. T. Si, and M. X. Yu. 1959. Bacteriophages from China. An ... Phage Mu. Cold Spring Harbor Press, Cold Spring Harbor, N.Y. OCLC 16089280, ISBN 0-87969-306-1 Ackermann, H.-W., and M. S. ... French; The Bacteriophage and its Behavior] OCLC 11981307 d'Hérelle, F., and G. H. Smith. 1926. The Bacteriophage and Its ... The Bacteriophages. Volume I Plenum Press, New York. OCLC 18686137 Calendar, R. 1988. The Bacteriophages. Volume II Plenum ...
Shapiro, James (April 1979). "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ...
Shapiro JA (April 1979). "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ... Mu X, Ahmad S, Hur S (2016). "Endogenous Retroelements and the Host Innate Immune Sensors". Advances in Immunology. 132: 47-69 ... Cloning vectors: These are types of hybrid plasmids with bacteriophages, used to transfer and replicate DNA . Fragments of DNA ...
gpT is a protein subunit of the phage head of phages similar to Bacteriophage Mu. This and other observations suggest that Mu- ... The Mu-like gpT Downstream Element RNA motif (Mu-gpT-DE RNA motif) is a conserved RNA structure that was discovered by ... The Mu-gpT-DE motif is only found in metagenomic sequences arising from unknown organisms. Mu-gpT-DE RNAs usually occur ... Thus, Mu-gpT-DE RNAs might function as small RNAs. Weinberg Z, Lünse CE, Corbino KA, Ames TD, Nelson JW, Roth A, Perkins KR, ...
Strong gyrase binding sites (SGS) were found in some phages (bacteriophage Mu group) and plasmids (pSC101, pBR322). Recently, ... Bacteriophage T4 gene 39. Nucleic Acids Res. 1986;14(19):7751-7765. doi:10.1093/nar/14.19.7751 Mufti S, Bernstein H. The DNA- ... doi:10.1128/JVI.14.4.860-871.1974 Hyman P. The genetics of the Luria-Latarjet effect in bacteriophage T4: evidence for the ... McCarthy D. Gyrase-dependent initiation of bacteriophage T4 DNA replication: interactions of Escherichia coli gyrase with ...
The high level of similarities in the tail fiber genes of phage P2, P1, Mu, λ, K3 and T2, which belong to different families, ... The P2-like bacteriophages. In R. Calendar (ed.), The bacteriophages. Oxford Press, Oxford, 2005: p. 365-390 Lindahl, G., ... Bacteriophage P2 was first isolated by G. Bertani from the Lisbonne and Carrère strain of E. coli in 1951. Since that time, a ... Bacteriophage P2, scientific name Escherichia virus P2, is a temperate phage that infects E. coli. It is a tailed virus with a ...
"Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu". ... Fis was first discovered for its role in stimulating Gin catalyzed inversion of the G segment of phage Mu genome. Fis was ... In addition to bringing about overall downregulation of the Mu genome, it also ensures silencing of the advantageous but ... as the factor for inversion stimulation of the homologous Hin and Gin site-specific DNA recombinases of Salmonella and phage Mu ...
Kahmann R, Rudt F, Koch C, Mertens G (July 1985). "G inversion in bacteriophage Mu DNA is stimulated by a site within the ... Kano Y, Goshima N, Wada M, Imamoto F (1989). "Participation of hup gene product in replicative transposition of Mu phage in ...
Eisenstark, Abraham (2014). "Life in Science: Abraham Eisenstark". Bacteriophage. 4 (3): e29009. doi:10.4161/bact.29009. PMC ... mu-1, a bacterial virus that has been important for understanding gene transposition and the development of molecular genetics ... the discovery that bacteriophage can transfer plasmid genes as well as chromosomal genes; and the establishment of the ...
... bacteriophage mu MeSH B04.123.150.500.300 - bacteriophage p1 MeSH B04.123.150.500.305 - bacteriophage p2 MeSH B04.123.150.500. ... bacteriophage mu MeSH B04.280.090.500.300 - bacteriophage p1 MeSH B04.280.090.500.305 - bacteriophage p2 MeSH B04.280.090.500. ... bacteriophage lambda MeSH B04.123.205.250 - bacteriophage m13 MeSH B04.123.205.260 - bacteriophage mu MeSH B04.123.205.280 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ...
"Inversion of the G segment of bacteriophage Mu: analysis of a genetic switch". His study focused on transposon sequences in DNA ...
Shapiro, J. A. (1979), "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ... "Transposition of phage Mu DNA", in Craig, N. L.; Craigie, R.; Gellert, M.; Lambowitz, A. M. (eds.), Mobile DNA II, American ...
... such as the Tn3 ampicillin resistance transposon and transposing bacteriophage Mu. In this model, the ends of transposable ... involved transduction to clone oppositely oriented copies of the gene inserted into two specialized transducing bacteriophages ...
... phage, a bacteriophage of the family Myoviridae of double-stranded DNA non-enveloped contractile tail bacterial viruses ... Look up MU, Mu, mu, 無, 木, 母, μ, or Μ in Wiktionary, the free dictionary. MU, Mu or μ may refer to: Aries Mu, a character from ... Mu Online, a 2003 online role-playing game Mu, an ancient civilization from Mega Man Star Force 2. Mu-12, a character from the ... metric prefix for one millionth Mu (kana), む or ム, a Japanese kana Mu (cuneiform), a sign in cuneiform writing Mu (negative), a ...
Bacteriophage Bacteriophage pRNA Φ29 DNA polymerase Padilla-Sanchez, Victor (2021-07-17), Bacteriophage Φ29 structural model at ... Zhang, Long; Mu, Chaofeng; Zhang, Tinghong; Yang, Dejun; Wang, Chenou; Chen, Qiong; Tang, Lin; Fan, Luhui; Liu, Cong; Shen, ... Bacillus virus Φ29 (bacteriophage Φ29) is a double-stranded DNA (dsDNA) bacteriophage with a prolate icosahedral head and a ... The pRNA in bacteriophage Φ29 can use its three-way junction in order to self-assemble into nanoparticles. One major challenge ...
CRISPR consists of genomic sequences that can be found in prokaryotic organisms, that come from bacteriophages that infected ... van Gent M, Gack MU (September 2018). "Viral Anti-CRISPR Tactics: No Success without Sacrifice". Immunity. 49 (3): 391-393. doi ... Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR (January 2013). "Bacteriophage genes that inactivate the CRISPR/Cas bacterial ... Borges AL, Zhang JY, Rollins MF, Osuna BA, Wiedenheft B, Bondy-Denomy J (August 2018). "Bacteriophage Cooperation Suppresses ...
Lessa FC, Mu Y, Bamberg WM, Beldavs ZG, Dumyati GK, Dunn JR, et al. (February 2015). "Burden of Clostridium difficile infection ... Treatment with bacteriophages directed against specific toxin-producing strains of C difficile are also being tested. A study ...
My first standing ovation': Humble MU professor cheered after winning Nobel Prize". Kansas City Star. Retrieved 7 October 2018 ... causing the protein to be expressed on the outside of the bacteriophage. Smith first described the technique in 1985 when he ... a technique where a specific protein sequence is artificially inserted into the coat protein gene of a bacteriophage, ...
"Bacteriophage P1", in Richard Calendar (ed.), The Bacteriophages, Oxford University Press, p. 350, ISBN 0195148509 Viralzone: ... This system has close sequence homologies to recombinational systems in the tail fibers of unrelated phages like the mu phage ... The genome of P1 encodes 112 proteins and 5 untranslated genes and is this about twice the size of bacteriophage lambda. The ... Sandmeier, H.; S. Iida; W. Arber (1992-06-01). "DNA Inversion Regions Min of Plasmid p15B and Cin of Bacteriophage P1: ...
... mu ={\frac {\ln 2}{t_{d}}}} Therefore, the doubling time td becomes a function of dilution rate D in steady state: t d = ln ⁡ 2 ... specific types of bacterial mutants in culture such as auxotrophs or those that are resistant to antibiotics or bacteriophages ... mu _{\max }{S \over K_{S}+S},} where S is the substrate or nutrient concentration in the chemostat and KS is the half- ...
Kresge, N., Simoni, R. D., and Hill, R. L. (July 13, 2007). "DNA Replication in Bacteriophage: the Work of Charles C. ... Pi Mu Epsilon A.M. (Hon), Harvard University, 1967 Elected Fellow, American Academy of Arts and Sciences, 1975 Elected Member, ... In 1998, Richardson examined the crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution. Before ... Kresge, Nicole; Simoni, Robert D.; Hill, Robert L. (July 13, 2007). "DNA Replication in Bacteriophage: the Work of Charles C. ...
A bacteriophage, Listeria phage P100, has been proposed as food additive to control L. monocytogenes. Bacteriophage treatments ... Ramaswamy V, Cresence VM, Rejitha JS, Lekshmi MU, Dharsana KS, Prasad SP, Vijila HM (February 2007). "Listeria--review of ... The U.S. Food and Drug Administration (FDA) approved a cocktail of six bacteriophages from Intralytix, and a one-type phage ... Carlton RM, Noordman WH, Biswas B, de Meester ED, Loessner MJ (December 2005). "Bacteriophage P100 for control of Listeria ...
H ( z , t ) = μ a I 0 e − ω t {\displaystyle H(z,t)=\mu _{a}I_{0}e^{-\omega t}} Where μ a {\displaystyle \mu _{a}} is the ... Although antibodies have been used in the past, bacteriophages have proven to be cheaper and more stable to produce. Multiple ... p ( z , t ) = μ a β F ν s 2 2 C p h ^ ( z , t ) {\displaystyle p(z,t)={\mu _{a}\beta F\nu _{s}^{2} \over 2C_{p}}{\hat {h}}(z,t ... Where μ a {\displaystyle \mu _{a}} is the absorption coefficient, is the thermal expansion coefficient, C p {\displaystyle C_{p ...
BRAY, D; ROBBINS, P (1967). "Mechanism of ε15 conversion studied with bacteriophage mutants". Journal of Molecular Biology. ... Borowitz JL, Gunasekar PG, Isom GE (September 1997). "Hydrogen cyanide generation by mu-opiate receptor activation: possible ...
The retention of bacteriophage and bacteria, the so-called "bacteria challenge test", can also provide information about the ... mu }}\ A\left({\frac {1}{R_{m}+R}}\right)} where Vp and Q are the volume of the permeate and its volumetric flow rate ...
These aforementioned techniques all require the design of mini-Mu transposons. Thermo scientific manufactures kits for the ... This method utilizes a bacteriophage with a modified life cycle to transfer evolving genes from host to host. The phage's life ...
Bacteriophages are able to infect most bacteria and are easily found in most environments colonized by bacteria, and have been ... Mu, Quanhua; Li, Jin; Sun, Yingxue; Mao, Daqing; Wang, Qing; Yi, Luo (5 December 2014). "Occurrence of sulfonamide-, ... Another research team was able to use bacteriocins, antimicrobial peptides and bacteriophages in the control of bacterial ... Joerger R.D. (2003). "Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages". Poultry Science. ...
Nobel Prize for bacteriophage genetics Jay Lush (1896-1982), US animal geneticist who pioneered modern scientific animal ... notable contributions to the study of phage Mu T. C. Hsu (1917-2003), distinguished Chinese-American cell biologist, geneticist ...
"Using a bacteriophage to successfully treat a patient infected with a drug-resistant bacteria". medicalxpress.com. Retrieved 14 ... Wang, Zhihang; Wu, Zhenhua; Hu, Zhiyu; Orrego-Hernández, Jessica; Mu, Erzhen; Zhang, Zhao-Yang; Jevric, Martyn; Liu, Yang; Fu, ... "Combination of pre-adapted bacteriophage therapy and antibiotics for treatment of fracture-related infection due to pandrug- ...
... mu ,\Sigma )={\frac {1}{\sqrt {(2\pi )^{N},\Sigma ,}}}\exp \left\{-{\frac {1}{2}}(x-\mu )^{T}\Sigma ^{-1}(x-\mu )\right\}} ( 2 ... Rader, AJ; Vlad, Daniel; Bahar, Ivet (2005). "Maturation Dynamics of Bacteriophage HK97 Capsid". Structure. 13 (3): 413-21. doi ...
Bacteriophage (phage) T4 encodes a DNA polymerase that catalyzes DNA synthesis in a 5' to 3' direction. The phage polymerase ... mu), and Terminal deoxynucleotidyl transferase (TdT). Family X polymerases are found mainly in vertebrates, and a few are found ... Gillin FD, Nossal NG (September 1976). "Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 ...
Bacteriophages can even possess the ability to digest the biofilm that many bacteria develop that protect them from antibiotics ... Ahmad A, Patel I, Khan MU, Babar ZU (June 2017). "Pharmaceutical waste and antimicrobial resistance". The Lancet. Infectious ... Bacteriophage cocktails are common drugs sold over the counter in pharmacies in eastern countries. In Belgium, four patients ... Phage therapy relies on the use of naturally-occurring bacteriophages to infect and lyse bacteria at the site of infection in a ...
Sahara Hot Start PCR Master Mix Berezovski MV, Lechmann M, Musheev MU, Mak TW, Krylov SN (July 2008). "Aptamer-facilitated ... Tuerk C, Gold L (August 1990). "Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA ...
Mosaicism Mouse model mRNA mtDNA Mu particle Mu phage Mullerian mimicry Multifactorial Multihybrid Multimeric structure ... mutant Axoneme B form DNA Bacillus Back mutation Backcross Bacteria Bacterial conjugation Bacterial lawn Bacteriophage Balbiani ...
... mu \, \over e}={\mu (N+\Delta \,N)-\mu (N) \over e}} may be applied to a quantum dot with the addition or removal of individual ... Genetically engineered M13 bacteriophage viruses allow preparation of quantum dot biocomposite structures. It had previously ... mu (N+1)-\mu (N)}={e^{2} \over I(N)-A(N)}} is the quantum capacitance of a quantum dot, where we denoted by I(N) the ionization ... mu a^{2}}}\\E_{\textrm {exciton}}&=-{\frac {1}{\epsilon _{\rm {r}}^{2}}}{\frac {\mu }{m_{\rm {e}}}}R_{y}=-R_{y}^{*}\\E&=E_{\ ...
Zhang, Yinong; van der Fits, Leslie; Voerman, Jane S.; Melief, Marie-Jose; Laman, Jon D.; Wang, Mu; Wang, Haitao; Wang, Minhui ... This peptidoglycan-binding type 2 amidase domain is homologous to bacteriophage and bacterial type 2 amidases. PGRP domain has ... Li, Xinna; Wang, Shiyong; Qi, Jin; Echtenkamp, Stephen F.; Chatterjee, Rohini; Wang, Mu; Boons, Geert-Jan; Dziarski, Roman; ... Wang, Zheng-Ming; Li, Xinna; Cocklin, Ross R.; Wang, Minhui; Wang, Mu; Fukase, Koichi; Inamura, Seiichi; Kusumoto, Shoichi; ...
Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from ...
Iino T, Kutsukake K. Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment involved in ... Iino, T. ; Kutsukake, K. / Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment involved ... Iino, T., & Kutsukake, K. (1981). Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment ... Iino, T & Kutsukake, K 1981, Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment ...
Whitworth C, Mu Y, Houston H, Martinez-Smith M, Noble-Wang J, Coulliette-Salmond A, Rose L.. Appl Environ Microbiol. 2020 Jun ... The bacteriophage Phi 6 has a phospholipid envelope and is commonly used in environmental studies as a surrogate for human ... Persistence of bacteriophage Phi 6 on porous and non-porous surfaces; potential for use as Ebola or coronavirus surrogate ...
In this perspective, we discuss several aspects of a characteristic feature of bacteriophages, their host range. Each phage has ... In this perspective, we discuss several aspects of a characteristic feature of bacteriophages, their host range. Each phage has ... Paolozzi, L., and Ghelardini, P. (2006). "The bacteriophage Mu," in The Bacteriophages, eds R. Calendar and S. T. Abedon ( ... Keywords: bacteriophage, host range, isolation protocol, phage therapy, bacteriophage ecology, bacteriophage evolution, phage- ...
bacteriophage Mu transposase PMID:7628012. Family alignment:. View or CHROMA format. HMM/Web logo. CLUSTALW format. MSF format ...
Bradley DE, Pitt TL: Pilus-dependence of four Pseudomonas aeruginosa bacteriophages with non-contractile tails. J Gen Virol ... Cazares A, Mendoza-Hernández G, Guarneros G: Core and accessory genome architecture in a group of Pseudomonas aeruginosa Mu- ... In the case of KRP1, the intact JBD93 bacteriophage, which was detected as GI 23, uses O-antigen mediated infection [31]. Since ... Roncero C, Darzins A, Casadaban M: Pseudomonas aeruginosa transposable bacteriophages D3112 and B3 require pili and surface ...
UNSPECIFIED (1987) INVESTIGATION OF THE POSSIBLE INVOLVEMENT OF CYCLIC-GMP IN THE TRANSPOSITION OF BACTERIOPHAGE MU AND OTHER ... 1987) 3-MU-M VLSI PROCESSING ELEMENT USING THE CORDIC ALGORITHM. ELECTRONICS LETTERS, 23 (21). pp. 1164-1166. ... 1987) A COMPARATIVE MUON SPIN RELAXATION (MU-SR) STUDY OF TITANIUM AND YTTRIUM DIHYDRIDES. JOURNAL OF THE LESS-COMMON METALS, ... 1987) UNSYMMETRICAL BRIDGING OF AN ACETYLIDE LIGAND IN THE CATION [PT2(C=CBUT)3(MU-DPPM)2]+ (DPPM = PH2PCH2PPH2) - X-RAY ...
In vivo mutational analysis of bacteriophage Mu operators. par Desmet, Lucie;Gama Fernandes Caldas, Maria-José ;Laachouch, ... In bacteria lysogenic for bacteriophage Mu, the phage repressor binds to a tripartite operator region, O1,O2,O3, to repress the ...
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency ...
A promoter probe, Tn5-VB32, was constructed and placed in a P group R plasmid containing bacteriophage Mu sequences, allowing ... N2 - A promoter probe, Tn5-VB32, was constructed and placed in a P group R plasmid containing bacteriophage Mu sequences, ... AB - A promoter probe, Tn5-VB32, was constructed and placed in a P group R plasmid containing bacteriophage Mu sequences, ... abstract = "A promoter probe, Tn5-VB32, was constructed and placed in a P group R plasmid containing bacteriophage Mu sequences ...
93; was PHAGE MU 1990-92; BACTERIOPHAGE MU was see PHAGE MU 1990-92, was see PHAGE MU 1 1980-89. ... 93; was PHAGE MU 1990-92; BACTERIOPHAGE MU was see PHAGE MU 1990-92, was see PHAGE MU 1 1980-89. ... Coliphage mu. Enterobacteria phage Mu. Phage mu. mu Phage. mu Phages. Tree number(s):. B04.123.150.500.260. B04.123.205.260. ... colifago mu fago mu fago mu de enterobacterias Scope note:. Colifago temperado, del género de virus similares a Mu, familia ...
Ner consists of bacteriophage mu, to a point mutation to mutations can produce. ...
Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. J A Shapiro. Proc. ... The best studied transposable bacterial virus is bacteriophage Mu. During replicative transposition, interactions between DDE- ... Mu insertions are repaired by the double-strand break repair pathway of Escherichia coli. Sooin Jang, Steven J Sandler, Rasika ... In vitro maturation and encapsidation of the DNA of transposable Mu-like phage D108. C M Burns, H L Chan, M S DuBow. Proc. Natl ...
The bacteriophage Mu transposase protein can form high-affinity protein-DNA complexes with the ends of transposable elements of ... Stimulation of the Mu A protein-mediated strand cleavage reaction by the Mu B protein, and the requirement of DNA nicking for ... Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of ... The Mu transpositional enhancer can function in trans: Requirement of the enhancer for synapsis but not strand cleavage ...
Bacteriophage_Mu,bacteriophage Mu]] ,ref,. Harshey RM, Bukhari AI. APPENDIX: A model for mu transposition. Cold Spring Harb ... Mu,bacteriophage Mu]] appeared to target replication forks ,ref,,pubmed,. PMC349492. ,/pubmed,,/ref,. In this model ([[:File: ... Shapiro JA . Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements. - Proc ... Fitts RA, Taylor AL . Integration of bacteriophage Mu at host chromosomal replication forks during lytic development. - Proc ...
Excision and duplication of su3+-transducing fragments carried by bacteriophage phi 80. I. Novel structure of phi 80sus2psu3+ ... Cloning of human immunoglobulin mu gene and comparison with mouse mu gene. scientific article published on December 1980 ... Cloning of human immunoglobulin mu gene and comparison with mouse mu gene (English) ... Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways ...
Udupa, S and Nagaraja, V and Karambelkar, S (2022) Binding Affinity Quantifications of the Bacteriophage Mu DNA Modification ...
The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku. EMBO Reports, 4 (1). pp. 47-52. ISSN 1469-221X ...
Silent mischief: Bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using ...
Invitro and Invivo Manipulations of Bacteriophage Mu DNA - Cloning of Mu Ends and Construction of Mini-Mus Carrying Selectable ... Bukhari, A. I., Dubow, M. S., Khatoon, H., Ambrosio, L., Debruijn, F., Ljungquist, E. (1979) Integration of bacteriophage mu ... Bukhari, A. I., Chaconas, G. C., Khatoon, H., Dubow, M. (1979) Replication and Integration of Bacteriophage Mu-DNA. Journal of ... Replication and Transposition of Phage Mu and Mini Mu DNA. Abstracts of the Annual Meeting of the American Society for ...
Gam of bacteriophage Mu. In Escherichia coli GamGFP forms foci at chromosomal DSBs and pinpoints their subgenomic locations. ... Bacteriófago mu/genética , Bacteriófago mu/metabolismo , Dano ao DNA , Corantes Fluorescentes/metabolismo , Proteínas Virais ... The N protein of phage Mu was indicated from studies in Escherichia coli to hold linear Mu chromosomes in a circular ... We compared N-GFP with a biochemically well documented DSB-end binding protein, the Gam protein of phage Mu, also fused to GFP ...
Species Bacteriophage mu [TaxId:10677] [46893] (4 PDB entries). More info for Family a.4.5.26: mu transposase, DNA-binding ... Timeline for Family a.4.5.26: mu transposase, DNA-binding domain: *Family a.4.5.26: mu transposase, DNA-binding domain appears ... Lineage for Family a.4.5.26: mu transposase, DNA-binding domain. *Root: SCOP 1.55 *. Class a: All alpha proteins [46456] (138 ... Family a.4.5.26: mu transposase, DNA-binding domain appears in the current release, SCOPe 2.08, called Family a.6.1.7: ...
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency ...
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T: A base editors with higher ... Xu K, Zhou Y, Mu Y, Liu Z, Hou S, Xiong Y, et al. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and ... In archaea and bacteria, CRISPRs function as a defense mechanism against bacteriophages via interactions with the Cas enzyme ... proteins create double stranded breaks in the genome of foreign DNA thus destroying the genome of the invading bacteriophage ( ...
386 Advanced Molecular Biology Host-range variation in bacteriophage Mu. The standard workweek is 45 hours and time worked in ...
Finallу, ᴡe deᴠeloped additional baѕe editorѕ-BE3-Gam, SaBE3-Gam, BE4-Gam, and SaBE4-Gam-that uѕe the bacteriophage Mu dѕDNA ( ... Fuѕing BE3, BE4, SaBE3, or SaBE4 to Gam, a bacteriophage Mu protein that bindѕ DSBѕ greatlу reduceѕ indel formation during baѕe ...
Bacteriophage Mu, Gp27 1 Domain IPR026354:Four helix bundle suffix domain 1 Domain IPR029100:Bacterial toxin 50 1 Family ... Bacteriophage Mu, Gp25 1 Family IPR006938:Protein of unknown function DUF624 1 Family IPR018592:Protein of unknown function ... Bacteriophage Mu, Gp16 1 Family IPR025445:Protein of unknown function DUF4191 1 Family IPR010100:TonB-dependent copper receptor ... Bacteriophage Mu-like, Gp48 2 Family IPR010846:Protein of unknown function DUF1460 2 Domain IPR021730:Dicarboxylate transport 2 ...
The fate of bacteriophages in recirculating aquaculture systems (RAS)-towards developing phage therapy for RAS. GMF Almeida, K ... S M ntynen, E Laanto, HM Oksanen, MM Poranen, SL D az-Mu oz ... High temperature and bacteriophages can indirectly select for ... Bacteriophage adherence to mucus mediates preventive protection against pathogenic bacteria. GMF Almeida, E Laanto, R Ashrafi, ... Bacteriophage adherence to mucus mediates preventive protection against pathogenic bacteria. mBio 10: e01984-19. GMF Almeida, E ...
... sites for bacteriophage glucose, 2000 Mol. download 700 Sundays band and invention construct mixed by Cre mortality in germinal ... The Gin model of paradigm Mu can recombine G-protein-coupled collagen in real-world data, 1991 Mol. ...
  • Because many bacteriophages kill the bacterial cells they infect, phages provide a possible alternative to antibiotics. (frontiersin.org)
  • The best studied transposable bacterial virus is bacteriophage Mu. (expasy.org)
  • Luria & Delbrück performed an experiment on bacteriophage resistance in Escherichia coli, growing bacterial cultures, exposing them to a phage, and then plating and counting the survivors, who have become resistant to the phage. (r-bloggers.com)
  • Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from phage DNA. (uab.edu)
  • In vitro transposition characteristics of mini-Mu plasmids carrying terminal base pair mutations. (mcmaster.ca)
  • Depending on the way the phage DNA nicked 3'-OH ends attack the target, the segment between the integrated viral genome and the target will be either deleted with excision of a chromosomal segment and one Mu DNA copy (binding to the same strand) or inverted (binding to the opposite strand, shown here). (expasy.org)
  • The Cas proteins create double stranded breaks in the genome of foreign DNA thus destroying the genome of the invading bacteriophage (Barrangou et al. (biomedcentral.com)
  • A family of bacteriophages that infects enterobacteria, CAULOBACTER, and PSEUDOMONAS. (jefferson.edu)
  • Bacteriophages are viruses that infect bacteria. (frontiersin.org)
  • A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE , composed of a linear, double-stranded molecule of DNA , which is able to insert itself randomly at any point on the host chromosome. (bvsalud.org)
  • This use of bacteriophages, called phage therapy, was the first use proposed for phage soon after their discovery ( Summers, 2012 ). (frontiersin.org)
  • Host range is a key property for phage therapy as well as the biology of bacteriophages in general. (frontiersin.org)
  • Stimulation of the Mu A protein-mediated strand cleavage reaction by the Mu B protein, and the requirement of DNA nicking for stable type 1 transpososome formation. (mcmaster.ca)
  • Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of stable binding of the Mu B protein to DNA. (mcmaster.ca)
  • Fuѕing BE3, BE4, SaBE3, or SaBE4 to Gam, a bacteriophage Mu protein that bindѕ DSBѕ greatlу reduceѕ indel formation during baѕe editing, in moѕt caѕeѕ to beloᴡ 1.5%, and further improᴠeѕ product puritу. (6struyenky.vn)
  • joint download in Human Cells Catalyzed by Phage transcription Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of paradigm Mu can recombine G-protein-coupled collagen in real-world data, 1991 Mol. (mid-southrealty.com)
  • Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment involved in flagellar phase variation of Salmonella. (elsevier.com)
  • The types (strains or species) of bacteria that a bacteriophage is able to infect is considered the host range of the phage in question ( Hyman and Abedon, 2010 ). (frontiersin.org)
  • The most studied bacteriophages are those that infect the Gram-positive bacterium Mycobacterium smegmatis mc 2 155, with over 4,800 phages isolated and 690 fully sequenced genomes ( http://www.phagesdb.org ). (biomedcentral.com)
  • This group of phages has been isolated and sequenced independently from investigators throughout the world and contains many of the well-characterized, historical phages such as Lambda, Mu, T4 and T7. (biomedcentral.com)
  • Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE . (nih.gov)
  • In archaea and bacteria, CRISPRs function as a defense mechanism against bacteriophages via interactions with the Cas enzyme family. (biomedcentral.com)
  • Excision and duplication of su3+-transducing fragments carried by bacteriophage phi 80. (wikidata.org)
  • For that purpose, we have explored an alternative method for the detection and quantification of bacteriophages that fulfills the criteria of being rapid, simple and inexpensive. (uab.cat)
  • A promoter probe, Tn5-VB32, was constructed and placed in a P group R plasmid containing bacteriophage Mu sequences, allowing transfer of the transposon to bacteria such as Caulobacter, Rhizobium, and Agrobacterium without retention of the plasmid. (researchwithrutgers.com)
  • Previous studies have shown that certain serological group B bacteriophages of S. aureus are capable of generalized transduction. (muni.cz)
  • Colifago temperado, del género de virus similares a Mu, familia MYOVIRIDAE, compuesto de una molécula de ADN lineal de doble hebra, que es capaz de insertarse aleatoriamente en cualquier punto del cromosoma del huésped. (bvsalud.org)
  • In this perspective, we discuss several aspects of a characteristic feature of bacteriophages, their host range. (frontiersin.org)
  • Nearly all bacteriophages have been isolated using a single host strain of bacteria. (frontiersin.org)
  • This challenges the common belief that most bacteriophages have a narrow host range. (frontiersin.org)
  • The construction is described of a plasmid (pL-ner) which directs the high-level production of the bacteriophage Mu Ner protein in Escherichia coli. (nih.gov)
  • 1998). Assembly of both the head and tail of bacteriophage Mu is blocked in Escherichia coli groEL and groES mutants. (tcdb.org)
  • Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophages lambda and Mu. (microbiologyresearch.org)
  • Integration host factor activates the Ner-repressed early promoter of transposable Mu-like phage D108. (nih.gov)
  • The phage Mu protein forms the portal vertex of the capsid ( Grimaud 1996 ). (tcdb.org)
  • After post-doctoral training at the University of California, San Francisco Medical Center, she joined Cold Spring Harbor Laboratory (1975) studying gene regulation by phage Mu DNA inversion and adenovirus transcription and replication. (uab.edu)
  • Certain bacterial transposons including phage Mu selectively transpose to target DNA sites away from where a pre-existing copy of the same transposon is located. (nih.gov)
  • Insertion of phage Mu. (springernature.com)
  • phage-display technology is a method for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages to connect proteins with the genetic information that encodes them. (missouri.edu)
  • Endolysin Regulation in Phage Mu Lysis. (tamu.edu)
  • Genome study of a novel virulent phage vB_SspS_KASIA and Mu-like prophages of Shewanella sp. (ddlemb.com)
  • She completed her undergraduate training in Agricultural Chemistry at National Taiwan University (1965) and carried out her graduate studies in Chemistry at the California Institute of Technology (Ph.D., 1973) in the lab of Norman Davidson, developing electron microscopic heteroduplex methods to study gene organization of bacteria and their bacteriophage lysogens. (uab.edu)
  • Viruses that grow in bacteria are sensitive to this structure, and when bacteriophage Mu replicates it transposes from one domain to another (Mana and Higgins, 1999). (uab.edu)
  • There are many recent studies regarding the efficacy of bacteriophage-related lytic enzymes: the enzymes of 'bacteria-eaters' or viruses that infect bacteria. (asmallerflea.org)
  • Interactions between Viral Regulatory Proteins Ensure an MOI-Independent Probability of Lysogeny during Infection by Bacteriophage P1. (tamu.edu)
  • This study also found that, among the eight physicochemical properties considered, the charge house has the greatest impact on the classification of bacteriophage proteins These results show that the model discussed in this paper is an important tool in bacteriophage protein research. (fgfr-inhibitors.com)
  • This one, the lambda integrase from a bacteriophage (PDB entry 1z1g ), uses a more complex mechanism than the Tn5 transposase. (rcsb.org)
  • Lambda mu(+)mu(-) decays. (edu.vn)
  • Lambda mu(+)mu(-) at low hadronic recoil. (edu.vn)
  • 2021. Application of bacteriophage-derived endolysins to combat streptococcal disease: current state and perspectives. (umd.edu)
  • A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE , composed of a linear, double-stranded molecule of DNA , which is able to insert itself randomly at any point on the host chromosome. (nih.gov)
  • A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE , which infects E. coli. (bvsalud.org)
  • We report the discovery, properties and complete sequence (46,365bp) of Min1, the first bacteriophage to be reported for the coryneform genus Microbacterium. (wormbase.org)
  • The protein was also shown to have in vitro biological activity, as measured by specific binding to a DNA fragment containing the consensus Ner-binding sequence, and in vivo biological activity as the protein produced by the pL-ner plasmid allowed lysogenic-like maintenance of a Mu prophage c mutant unable to synthesise a functional Mu repressor. (nih.gov)
  • Dr. Jodi Vogel received her Ph.D. for her work on functions of the bacteriophage Mu repressor protein. (nih.gov)
  • Szatmari, GB , Kahn, J & DuBow, MS 1986, ' Orientation and sequence analysis of right ends and target sites of bacteriophage Mu and D108 insertions in the plasmid pSC101 ', Gene , vol. 41, no. 2-3, pp. 315-319. (elsevier.com)
  • Apparently, all of the detectable precise and most of the imprecise excision events require functioning of the Mu A gene. (wikigenes.org)
  • by using data published elsewhere on the physical length of the deletion intervals it can be concluded that about 25% of these Mu insertions are clustered in a segment representing 3% of the gene, and another 20% in a segment representing 2 to 8% of the gene. (springernature.com)
  • Dynamic protein interactions in the bacteriophage T4 replisome. (neb.com)
  • Bacteriophage protein-protein interactions. (nih.gov)
  • Sheep in wolves' clothing: Temperate T7-like bacteriophages and the origins of the Autographiviridae. (tamu.edu)
  • 112 - Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage. (nih.gov)
  • A series of deletions, Mu insertions and point mutations affecting the malK-lamB operon have been isolated. (springernature.com)
  • Further electron microscopic studies on the infection process of the lipid-containing bacteriophage ϕ 6. (microbiologyresearch.org)
  • J. M. Palmada and J. Ripoll , Optimal latent period in a bacteriophage population model structured by infection-age, Mathematical Models and Methods in Applied Sciences , 21 (2011), 693-718. (aimsciences.org)
  • The exact sites of insertion of two of the D108 prophages, as well as the Mu prophage, have been determined by sequence analysis. (elsevier.com)
  • This paper summarizes the history of bacteriophage therapy, its bacterial lytic mechanisms, and the studies that have focused on Enterobacteriaceae and bacteriophage therapy. (asmallerflea.org)
  • Bacteriophage Mu head assembly. (tcdb.org)
  • New Insights into the Structure and Assembly of Bacteriophage P1. (tamu.edu)
  • Bacteriophage therapy is an efficient alternative to antibiotics as it employs a different antibacterial mechanism. (asmallerflea.org)
  • Dr. Smith is the first MU Professor to receive a Nobel Prize for research conducted at the university. (missouri.edu)
  • The uniqueness of bacteriophages plays a significant role in bioinformatics research. (fgfr-inhibitors.com)
  • The test forces the future physician to go beyond just determining a diagnosis and to focus on behaviors that play an essential role to the effectiveness of the care he or she provides," said Kimberly Hoffman, Ph.D, associate dean for curriculum and assessment, and research associate professor of family and community medicine at MU. (medicalxpress.com)
  • In this issue, readers will not only find that bacteriophage research is a booming field but also learn about the diverse applications currently being explored for bacteriophages. (asmallerflea.org)
  • We suggest that a block in large-scale Mu replication allows the excision process to proceed. (wikigenes.org)
  • Solvent Extraction of Klebsiella pneumoniae Bacteriophage Lysates with 1-Dodecanol Results in Endotoxin Reduction with Low Risk of Solvent Contamination. (tamu.edu)
  • As results, the prototype device worked with a few nanoliters of Rhodamine 6G dye molecules in ethanol solution and showed a laser threshold of similar to 15 [mu]J/mm(2) when optically pumped with a frequency doubled Nd:YAG laser at 532 nm wavelength. (universite-paris-saclay.fr)
  • Hoffman is the author of a study describing how the assessment tool for patient-centered care was developed at the MU School of Medicine. (medicalxpress.com)
  • By defining these detailed, specific patient-centered behaviors, the researchers have been able to tailor the educational experience at the MU School of Medicine to help students gain these skills. (medicalxpress.com)
  • students must perform at a satisfactory level on the patient-centered care exam to graduate from the MU School of Medicine. (medicalxpress.com)
  • Recently, MU researchers have developed a credible tool to assess whether medical students have learned and are applying specific behaviors that characterize patient-centered care. (medicalxpress.com)
  • MU is an equal opportunity/access/affirmative action/pro-disabled and veteran employer and does not discriminate on the basis of sex in our education programs or activities, pursuant to Title IX and 34 CFR Part 106. (missouri.edu)
  • There are five types of heavy chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and mu) constant domains (CH1 to CH4). (embl.de)
  • Regulatory factors acting at the bacteriophage Mu middle promoter. (nih.gov)