Bacteriophage mu: A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.Bacteriophages: Viruses whose hosts are bacterial cells.Coliphages: Viruses whose host is Escherichia coli.Lysogeny: The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.Bacteriophage T4: Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Bacteriophage lambda: A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.Viral Proteins: Proteins found in any species of virus.Bacteriophage T7: Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.Genes, Viral: The functional hereditary units of VIRUSES.Integration Host Factors: Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.Transposases: Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.T-Phages: A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.Bacteriophage phi 6: Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.F Factor: A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Bacteriophage phi X 174: The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.Viral Regulatory and Accessory Proteins: A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.Bacteriophage P2: A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.DNA Replication: The process by which a DNA molecule is duplicated.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Bacteriophage M13: Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.DNA Viruses: Viruses whose nucleic acid is DNA.Endopeptidase Clp: An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.Bacteriophage T3: Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Bacteriophage Typing: A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.Bacteriophage P1: A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Salmonella Phages: Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.Siphoviridae: A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.Nucleotidyltransferases: A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Genes, Bacterial: The functional hereditary units of BACTERIA.Bacterial Proteins: Proteins found in any species of bacterium.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Factor For Inversion Stimulation Protein: A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.RNA Phages: Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.Bacteriolysis: Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.Bacteriophage PRD1: Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.Pseudomonas Phages: Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.Staphylococcus Phages: Viruses whose host is Staphylococcus.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Bacillus Phages: Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Podoviridae: A family of bacteriophages which are characterized by short, non-contractile tails.Receptors, Opioid, mu: A class of opioid receptors recognized by its pharmacological profile. Mu opioid receptors bind, in decreasing order of affinity, endorphins, dynorphins, met-enkephalin, and leu-enkephalin. They have also been shown to be molecular receptors for morphine.Streptococcus Phages: Viruses whose host is Streptococcus.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Viral Tail Proteins: Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Levivirus: A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.Gene Expression Regulation, Viral: Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.Molecular Weight: The sum of the weight of all the atoms in a molecule.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.DNA Packaging: The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.

An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction. (1/284)

A highly efficient DNA sequencing strategy was developed on the basis of the bacteriophage Mu in vitro DNA transposition reaction. In the reaction, an artificial transposon with a chloramphenicol acetyltransferase (cat) gene as a selectable marker integrated into the target plasmid DNA containing a 10.3-kb mouse genomic insert to be sequenced. Bacterial clones carrying plasmids with the transposon insertions in different positions were produced by transforming transposition reaction products into Escherichia coli cells that were then selected on appropriate selection plates. Plasmids from individual clones were isolated and used as templates for DNA sequencing, each with two primers specific for the transposon sequence but reading the sequence into opposite directions, thus creating a minicontig. By combining the information from overlapping minicontigs, the sequence of the entire 10,288-bp region of mouse genome including six exons of mouse Kcc2 gene was obtained. The results indicated that the described methodology is extremely well suited for DNA sequencing projects in which considerable sequence information is on demand. In addition, massive DNA sequencing projects, including those of full genomes, are expected to benefit substantially from the Mu strategy.  (+info)

SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA). (2/284)

In bacteria, SsrA RNA recognizes ribosomes stalled on defective messages and acts as a tRNA and mRNA to mediate the addition of a short peptide tag to the C-terminus of the partially synthesized nascent polypeptide chain. The SsrA-tagged protein is then degraded by C-terminal-specific proteases. SmpB, a unique RNA-binding protein that is conserved throughout the bacterial kingdom, is shown here to be an essential component of the SsrA quality-control system. Deletion of the smpB gene in Escherichia coli results in the same phenotypes observed in ssrA-defective cells, including a variety of phage development defects and the failure to tag proteins translated from defective mRNAs. Purified SmpB binds specifically and with high affinity to SsrA RNA and is required for stable association of SsrA with ribosomes in vivo. Formation of an SmpB-SsrA complex appears to be critical in mediating SsrA activity after aminoacylation with alanine but prior to the transpeptidation reaction that couples this alanine to the nascent chain. SsrA RNA is present at wild-type levels in the smpB mutant arguing against a model of SsrA action that involves direct competition for transcription factors.  (+info)

Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. (3/284)

A bipartite enhancer sequence (composed of the O1 and O2 operator sites) is essential for assembly of the functional tetramer of phage Mu transposase (MuA) on supercoiled DNA substrates. A three-site interaction (LER) between the left (L) and right (R) ends of Mu (att sites) and the enhancer (E) precedes tetramer assembly. We have dissected the role of the enhancer in tetramer assembly by using two transposase proteins that have a common att site specificity, but are distinct in their enhancer specificity. The activity of these proteins on substrates containing hybrid enhancers reveals a 'criss-crossed' pattern of interaction between att and enhancer sites. The left operator, O1, of the enhancer interacts specifically with the transposase subunit at the R1 site (within the right att sequence) that is responsible for cleaving the left end of Mu. The right operator, O2, shows a preferential interaction with the transposase subunit at the L1 site (within the left att sequence) that is responsible for cleaving the right end of Mu.  (+info)

Natural synthesis of a DNA-binding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi. (4/284)

We have identified a 34 kDa DNA-binding protein with an HU-like activity in the Lyme disease spirochete Borrelia burgdorferi. The 34 kDa protein is translated from an abundant transcript initiated within the gene encoding the A subunit of DNA gyrase. Translation of the 34 kDa protein starts at residue 499 of GyrA and proceeds in the same reading frame as full-length GyrA, resulting in an N-terminal-truncated protein. The 34 kDa GyrA C-terminal domain, although not homologous, substitutes for HU in the formation of the Type 1 complex in Mu transposition, and complements an HU-deficient strain of Escherichia coli. This is the first example of constitutive expression of two gene products in the same open reading frame from a single gene in a prokaryotic cellular system.  (+info)

Replacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication. (5/284)

The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108 allowed efficient Mu replication and was shown to contain a strong gyrase site. However, neither the strong gyrase site from the plasmid pSC101 nor the major gyrase site from pBR322 could promote efficient Mu replication, even though the pSC101 site is a stronger gyrase site than the Mu SGS as assayed by cleavage in the presence of gyrase and the quinolone enoxacin. To look for SGS-like sites in the Escherichia coli chromosome which might be involved in organizing nucleoid structure, fragments of E. coli chromosomal DNA were substituted for the SGS: first, repeat sequences associated with gyrase binding (bacterial interspersed mosaic elements), and, second, random fragments of the entire chromosome. No fragments were found that could replace the SGS in promoting efficient Mu replication. These results demonstrate that the gyrase sites from the transposing phages possess unusual properties and emphasize the need to determine the basis of these properties.  (+info)

Organization and dynamics of the Mu transpososome: recombination by communication between two active sites. (6/284)

Movement of transposable genetic elements requires the cleavage of each end of the element genome and the subsequent joining of these cleaved ends to a new target DNA site. During Mu transposition, these reactions are catalyzed by a tetramer of four identical transposase subunits bound to the paired Mu DNA ends. To elucidate the organization of active sites within this tetramer, the subunit providing the essential active site DDE residues for each cleavage and joining reaction was determined. We demonstrate that recombination of the two Mu DNA ends is catalyzed by two active sites, where one active site promotes both cleavage and joining of one Mu DNA end. This active site uses all three DDE residues from the subunit bound to the transposase binding site proximal to the cleavage site on the other Mu DNA end (catalysis in trans). In addition, we uncover evidence that the catalytic activity of these two active sites is coupled such that the coordinated joining of both Mu DNA ends is favored during recombination. On the basis of these results, we propose that the DNA joining stage requires a cooperative transition within the transposase-DNA complex. The cooperative utilization of active sites supplied in trans by Mu transposase provides an example of how mobile elements can ensure concomitant recombination of distant DNA sites.  (+info)

Identification of SoxS-regulated genes in Salmonella enterica serovar typhimurium. (7/284)

Salmonella enterica serovar Typhimurium responds to superoxide-generating agents through soxR-mediated activation of the soxS gene, whose product, SoxS, is necessary for resistance to oxidative stress. The S. enterica serovar Typhimurium soxRS system also mediates redox-inducible resistance to diverse antibiotics, which may be relevant to clinical infections. In order to identify SoxS-regulated genes in S. enterica serovar Typhimurium, a lacI-regulated expression system for the S. enterica serovar Typhimurium soxS gene was developed. This system was used to demonstrate that soxS expression is sufficient for the induction of resistance to the superoxide-generating drug paraquat and for the transcriptional activation of the sodA and micF genes. In addition, a library of random lacZ insertions was generated and screened for clones displaying differential beta-galactosidase activity in the presence or absence of SoxS. This selection yielded six independent chromosomal lacZ transcriptional fusions that were activated by either artificial expression of SoxS or exposure of wild-type cells to micromolar concentrations of paraquat. Moreover, disruption of the inducible genes by the insertions rendered S. enterica serovar Typhimurium hypersensitive to millimolar concentrations of paraquat. Nucleotide sequence determination identified the disrupted genes as sodA (Mn-containing superoxide dismutase), fpr (NADPH:ferredoxin oxidoreductase), and ydbK (a putative Fe-S-containing reductase).  (+info)

Mu DNA reintegration upon excision: evidence for a possible involvement of nucleoid folding. (8/284)

Mutations induced by the integration of a Mugem2ts prophage can revert at frequencies around 1x10(-6). In these revertant clones, the prophage excised from its original localization is not lost but reintegrated elsewhere in the host genome. One of the most intriguing aspects of this process is that the prophage reintegration is not randomly distributed: there is a strong correlation between the original site of insertion (the donor site) and the target site of the phage DNA migration (the receptor site). In this paper, it is shown that in the excision-reintegration process mediated by Mugem2ts, the position of the initial prophage site strongly influences the location of the reintegration site. In addition, for each donor site, the receptor site is a discrete DNA region within which the excised Mu DNA can reintegrate and the two sites implicated in phage DNA migration must be located on the same DNA molecule. These data suggest the involvement of nucleoid folding in the excision-reintegration process.  (+info)

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Transposition allows movement of a defined stretch of DNA, a transposon, from one DNA location to another. This process is required for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic resistances between bacterial populations; and it is responsible for spontaneous mutations in all the kingdoms of life. Transposition is mediated by a protein, the transposase, encoded by the transposon. DNA sequence signals at the two ends of the transposon activate assembly of a transpososome: a complex that include multiple copies of transposase plus both transposon ends. Transpososome assembly, in turn, activates the DNA cleavage and joining reactions required for transposition. This thesis explores aspects of interactions between one transposase, MuA, and the ends of its transposon DNA, the genome of bacteriophage Mu. The first chapter provides an overview of Mu transposition, with special emphasis on the transpososome. The second chapter shows that in the absence of ...
1BCM: Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.
E. coli ClpX is a member of the Clp/Hsp100 family of ATPases that remodel multi-component complexes and facilitate ATP-dependent protein degradation. Protein remodelers alter the biological activity of their substrates, typically by changing the quaternary structure of their target proteins. ClpX remodels protein-DNA complexes, termed transpososomes, made during recombination of the phage Mu. When recombination is complete, the core four-subunit transpososome complex does not spontaneously release the DNA; transposase remains so stably bound that subsequent replication of the Mu genome is inhibited. To understand how ClpX releases the replication block without destroying the transpososomes, we characterized the mechanism and products of transpososome remodeling. To better understand the mechanism ClpX uses to facilitate remodeling, I first participated in a collaborative project that defined major biochemical reaction steps involved in protein degradation by ClpX and its associated peptidase ...
Background: Tangle analysis has been applied successfully to study proteins which bind two segments of DNA and can knot and link circular DNA. We show how tangle analysis can be extended to model any stable protein-DNA complex. -- Results: We discuss a computational method for finding the topological conformation of DNA bound within a protein complex. We use an elementary invariant from knot theory called colorability to encode and search for possible DNA conformations. We apply this method to analyze the experimental results of Pathania, Jayaram, and Harshey (Cell 2002). We show that the only topological DNA conformation bound by Mu transposase which is biologically likely is the five crossing solution found by Pathania et al (although other possibilities are discussed). -- Conclusion: Our algorithm can be used to analyze the results of the experimental technique described in Pathania et al in order to determine the topological conformation of DNA bound within a stable protein-DNA complex ...
Using the Tukey method for each of the six comparisons yields: $$ \begin{eqnarray} 0.29 \le & \mu_2 - \mu_1 & \le 4.47 \\ & & \\ 1.13 \le & \mu_3 - \mu_1 & \le 5.31 \\ & & \\ -2.25 \le & \mu_1 - \mu_4 & \le 1.93 \\ & & \\ -2.93 \le & \mu_2 - \mu_3 & \le 1.25 \\ & & \\ 0.13 \le & \mu_2 - \mu_4 & \le 4.31 \\ & & \\ 0.97 \le & \mu_3 - \mu_4 & \le 5.15 \\ \end{eqnarray ...
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CEACAM 5 + 6小鼠单克隆抗体[MUS](ab4539)可与人样本反应并经WB, ELISA, IHC, Flow Cyt实验严格验证,被4篇文献引用。所有产品均提供质保服务,中国75%以上现货。
பகல் பன்னிரண்டு மணிக்கு மீண்டும் திருச்சூரை நோக்கிப் புறப்பட்டோம். திருச்சூரைக் கடந்து குன்னங்குளம் என்ற ஊரை நோக்கி எங்கள் மகிழ்வுந்து பறந்தது. நல்ல சாலை அமைப்பு என்பதால் திட்டமிட்டதை விட வேகமாக நாங்கள் திருச்சூரைக் கடந்தோம். வழியில் அண்ணன் வீரக்குமரன் அவர்களின் விரிவுரை மகிழ்வுந்தில் அமைந்தது. முனைவர் வீரக்குமரன் அவர்கள் தமிழ், ஆங்கிலம், மலையாளம் என ...
Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The contributions from the secondary structure of the transcriptional activator protein C of bacteriophage Mu to its specific DNA binding and the influence of various factors, viz ., electrolytes, and minor groove and major groove binders on this protein-DNA interaction have been addressed . Circular dichroism (CD) spectral results suggest that, in the absence of Mg2+, C protein exhibits a j3-pleated sheetlike structure and Mg2+ changes the conformation to a more a-helical structure which could provide specific geometrical constraints complementary to those of DNA helix. Thus, Mg 2+ acts as a cofactor for the binding of the C protein to its specific site in DNA by inducing conformational changes in the protein. Competitive binding studies with minor and major groove binding drugs, viz ., distamycin A and methyl green, respectively, and the DMS footprinting data indicate that the C protein recognizes the major groove of DNA during complex formation . Further, upon major groove binding, C protein ...
Like AZT, 1NP also inhibited 4MU glucuronidation by UGT2B7 without a substantial effect on substrate cooperativity (at least at low 1NP concentrations). However, inhibition resulted mainly from a decrease in Vmax. The model used to describe these data again assumes binding of inhibitor at a distinct effector site but with abolition of product formation from enzyme complexes containing inhibitor. As is evident from Fig. 7, A and C, rate of product formation approaches zero at high modifier concentration. Although experimental data were well described by the three-site model, the increase in S50 and decrease in Hill coefficient observed at the highest modifier concentrations suggests that 1NP may also act as a competitive inhibitor of 4MU glucuronidation.. In contrast to the inhibition of 4MU glucuronidation by 1NP, 4MU activated the formation of 1NPG by UGT2B7. Kinetic data were well described by the three-site model illustrated in Fig. 2B, where modifier binds at an effector site that is ...
Having professional family photos taken can be an ordeal, but its basically a necessary one to endure. Because at the end of the day, who doesnt love having a perfect-looking family portrait to hang on the wall?. Unless youre a member of the Zaring family, that is. Because their recent family photos are going viral for being hilariously awful.. Pam Zaring is a mom who just wanted some nice family pictures like any mom does. So she sought out the services of a professional photographer in her area, paid $250 for said services, and gathered her entire family and two dogs together for some scenic photos on a warm, sunny day.. The results? OMG. You just have to see them for yourself. You. Will. Die.. LOL forever. When family pictures go wrong, usually its because a kid is crying or someones eyes are closed. In this case, its the, uh, interesting editing choices.. "Please see these FOR REAL photos she delivered to us," Zaring wrote in the post. "She said the shadows were really bad on the ...
Question - what is the best way for the treatment for mu tumer and who - 5Y. Find the answer to this and other Neurology questions on JustAnswer
TY - JOUR. T1 - (+)-CC-1065 as a structural probe of Mu transposase-induced bending of DNA. T2 - Overcoming limitations of hydroxyl-radical footprinting. AU - Ding, Zhi Ming. AU - Harshey, Rasika M.. AU - Hurley, Laurence. PY - 1993/9/11. Y1 - 1993/9/11. N2 - Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90°. To probe the directionality and locus of these A-protein-induced bends, we have used the antitumor antibiotic (+)-CC-1065 as a structural probe. As a consequence of binding within the minor groove, (+)-CC-1065 is able to alkylate N3 of adenine in a sequence selective manner. This selectivity is partially determined by conformational flexibility of the DNA sequence, and the covalent adduct has a bent DNA structure in ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Abrahams, P. J., Mulder, C., Vandevoorde, A., Warnaar, S. O., Vandereb, A. J. (1975) Transformation of Primary Rat-Kidney Cells by Fragments of Simian Virus 40 DNA. Journal of Virology, 16 (4). pp. 818-823. Allet, Bernard, Bukhari, Ahmad I. (March 1975) Analysis of Bacteriophage Mu and Lambda-Mu Hybrid Dnas by Specific Endonucleases. Journal of Molecular Biology, 92 (4). pp. 529-536. Apte, B. N., Rhodes, H., Zipser, D. (September 1975) Mutation blocking the specific degradation of reinitiation polypeptides in E. coli. Nature, 257 (5524). pp. 329-31. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of Terminal Repetition in Adenovirus 2 DNA. Cold Spring Harbor Symposia on Quantitative Biology, 39. pp. 401-407. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of terminal repetition in adenovirus 2 DNA. Cold Spring Harb Symp Quant Biol, 39 Pt . pp. 401-7. Atkins, J. F., Gesteland, R. F. (August 1975) The synthetase gene of the RNA phages R17, MS2 and f2 has a single UAG terminator ...
Indicatii: Eliminarea parazitilor intestinali in cure de 7 zile repetate dupa o pauza de alte 7 zile pentru prevenirea recidivelor. Femelele măsoară până la zece milimetri în lungime și ajung să efectueze ouatul spre rect, unde cu mușcăturile lor provoacă prurit enervant pe marginea anusului. Apasă pentru a vedea definiția originală «oxiuro» în dicționarul Spaniolă dictionary.
Konsolidovaný počiatočný finančný výkaz Eurosystému k 1. januáru 2011a konsolidovaný finančný výkaz Eurosystému k 7. januáru 2011
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Diabetik je človek trpiaci cukrovkou a jeho život sa chorobe musí prispôsobiť. Pokiaľ sa diabetik riadi radami lekárov a dodržuje liečbu, môže prežuť plnohodnotný život.
To say gin is having a moment would underestimate its popularity, and they can be as varied as they are delicious: fruity, floral, spicy or herbal. Whatever your preference, theres a gin for you - but tonic is another story. Find out more about how tonic is made and how to match it to your gin.
+Analysis andIsolation ofPlasminogenActivator fromMammalian CellCulture BrothDelaine Zayas-Bazán BurgosJohn Muñoz TorresVibha Bansal PhDMr. Javier RosadoMr. Ca…
Transposase is an enzyme that binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. The word "transposase" was first coined by the individuals who cloned the enzyme required for transposition of the Tn3 transposon. The existence of transposons was postulated in the late 1940s by Barbara McClintock, who was studying the inheritance of maize, but the actual molecular basis for transposition was described by later groups. McClintock discovered that pieces of the chromosomes changed their position, jumping from one chromosome to another. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed. Transposition in maize causes changes in color; however, in other organisms, such as bacteria, it can cause antibiotic resistance. Transposition is also important in creating genetic diversity within species and adaptability to ...
SCOTTSDALE, Ariz. - August marks a significant 10-year anniversary for patients with digestive problems. Just one decade ago, in August 2001, the FDA approved use of capsule endoscopy technology to examine the last frontier of the human GI tract - the small bowel.. Mayo Clinic in Arizona was among the first medical centers in the U.S. to perform exams of the small bowel using the pioneering "pill camera" technology that can scan the far corners of the GI tract - painlessly and without surgery.. Over the past decade, Mayo Clinic as a three-site organization (Arizona, Florida and Minnesota) has completed more than 7,000 such exams, where patients swallow a vitamin-sized capsule that contains a miniature camera. The capsule then travels through the digestive tract, snapping thousands of pictures that are transmitted to a recorder that patients wear around their waist for eight to 12 hours. Images saved on the recorder are transferred to a computer to create a "video" of the digestive tract, ...
Zimwe mu nzara zizwi zabayeho, hari inzara ya Rukungugu yabaye ku ngoma yUmwami Yuhi IV Gahindiro. Iyo nzara yabaye ahagana mu ntangiriro zikinyejana cya XIX. Yatewe namapfa yiganje mu gihugu. Nkuko Padiri Alexis Kagame abyandika mu gitabo cye yise « Un Abrégé de lhistoire du Rwanda », Umwami Gahindiro nabiru bakoresheje icyo gihe Inzira yubwiru ya Rukungugu mu kuyirwanya. Iyo nzara yamaze umwaka umwe.. Nyuma yaho, mu gihe cyubukoloni bwAbadage, mu Rwanda habaye nizindi nzara, zirimo Ruyaga yo mu 1897-1903, Rwakabaga cyangwa se Kimwaramwara yo mu 1906-1909. Inzara Ruyaga yatewe ninzige zateye imyaka, zikayona kugeza aho bivuyemo inzara. Naho Inzara ya Rwakabaga yatewe namapfa yiganje cyane mu majyaruguru no mu burasirazuba bwigihugu.. Mu gihe cyubukoloni bwAbabiligi, u Rwanda rwatewe nizindi nzara zikomeye. Izo ni nka Rumanura cyangwa se Rumanurimbaba yo mu ntambara ya mbere yisi yose. Iyo nzara yabaye hagati yi 1916 ni 1918. Yatewe nintambara, ihunga ryabantu, ubusahuzi ...
This paper derives (by a new method) an equation due to Macdonald for determining the zeros of the associated Legendre functions of order m and non-integral degree n when the argument is close to -1. A closed form solution is obtained for the values of Q subscript n superscript m (mu) and Q subscript n superscript -m (mu) for mu close to 1. Certain observations are made concerning errors in a recently published article.(*RADAR CROSS SECTIONS
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OMA MUA (FI) i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för OMA MUA (FI).
Bukeye umukobwa numuhungu, mu masaha yigicamunsi barahura ngo batembere, sinzi uko umuhungu yaje kurabukwa igishyimbo mu menyo yumukobwa, umuhungu aba uwo munsi yari afite twa duti dukura ibiryo mu menyo, amuha kamwe agira ati " Akira ukure ifiriti mu menyo". ...
How many moms were...... - Just thought it would be intersting to see how many moms were high risk, why and how many week you were when you... -...
Worried mom - Im new to this site, have many questions so please be patient. My daughter is 19 and is dating a 17 year old is this normal,...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Shh! Three reasons why you need to be a sneaky mom. Hint -- it will help you look out for your children with even more confidence.
Rihanna seems to do pretty much exactly what she wants to do in life, without any pesky inhibitions, but it does seem that she has one person who scol...
The enteric bacterium Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo only under anaerobic growth conditions. We initiated a genetic analysis of the cobalamin biosynthetic (cob) gene cluster, Stable cob::lac operon fusions were generated by insertions of a transposition-defective derivative of bacteriophage Mu d1 (Ap lac) into the cob genes. beta-Galactosidase synthesis was repressed in the presence of exogenously supplied cyanocobalamin, demonstrating that the cobalamin biosynthetic pathway was regulated by end-product repression. Transcriptional polarity studies showed that the cob genes responsible for synthesis of the corrinoid intermediate cobinamide (branch I of the pathway) were organized into a single operon. Genes for the synthesis of 5,6-dimethylbenzimidazole (branch II) and the final assembly of the complete cobalamin molecule (branch III) were organized into two or more additional operons. All of the known cob genes (in branches I, II, and III) were transcribed in ...
Reproduction. Scope Note Narrower Term: Cattle Reproduction Narrower Term: Cell nuclei Transplantation Narrower Term: Conjugation (Biology) See Also: Embryology Narrower Term: Fertility Narrower Term: Fertilization (Biology) Narrower Term: Fetus Narrower Term: Gametogenesis See Also: Generative organs Narrower Term: Gynogenesis Narrower Term: Human reproduction Narrower Term: Isolating mechanisms Narrower Term: Ovulation Narrower Term: Paedogenesis Narrower Term: Parthenogenesis Narrower Term: Parthenogenesis in animals Narrower Term: Parturition Narrower Term: Pregnancy Narrower Term: Sexual cycle Narrower Term: Spawning Narrower Term: Spontaneous generation See Also: Theriogenology Narrower Term: Viruses Reproduction Narrower Term: Viviparity Narrower Term: Domestic animals Reproduction Narrower Term: Livestock Reproduction Narrower Term: Bacteriophage mu Reproduction Narrower Term: Oncogenic DNA viruses Reproduction Narrower Term: Herpes simplex virus Reproduction Narrower Term: Herpesviruses ...
N15likevirus (synonym N15-like viruses) is a genus of viruses in the order Caudovirales, in the family Siphoviridae. Bacteria serve as natural hosts, with transmission achieved through passive diffusion. There is currently only one species in this genus: the type species Enterobacteria phage N15. Group: dsDNA Order: Caudovirales Family: Siphoviridae Genus: N15likevirus Enterobacteria phage N15 N15likeviruses are nonenveloped, with a head and tail. The head is about 60 nm in diameter. The tail is long and flexible, at about 140 nm long, 8 nm wide, with short brush-like terminal fibers. Enterobacteria phage N15 has been fully sequenced. It has about 46k nucleotides, with 60 proteins. The complete genome is available here Viral replication is cytoplasmic. The virus attaches to the host cells adhesion receptors using its terminal fiber, and ejects the viral DNA into the host cytoplasm via long flexible tail ejection system. Replication follows the replicative transposition model. DNA-templated ...
These results indicate that the mouse Fv1 gene has had antiviral activity since shortly after its introduction into the Mus genome, an event that occurred during a period of rapid diversification that gave rise to the 4 Mus subgenera ≈7 MY ago (10, 23). That this sequence has long had antiviral function is supported by the observation that the Fv1 genes of 2 species of Nannomys have novel Fv1-mediated antiviral phenotypes, and by the demonstration that Fv1 has been under positive Darwinian selection. We identified 2 segments of Fv1 potentially involved in restriction: an MHR segment known to function in capsid binding, and 3 residues in the C-terminal domain (CTD) of which 2 are known to contribute to the specificity of Fv1 restriction in laboratory mice.. The mechanism of Fv1 restriction has not been elucidated after 40 years of investigation, but the determination that Fv1 encodes a retroviral capsid-like protein suggests that Fv1 may bind capsids of exogenous virus and interfere with capsid ...
Newcastle University and Arthritis UK have announced a major new research centre that aims to find out more about the causes of rheumatoid arthritis.. The £2.5m Arthritis Research UK Rheumatoid Arthritis Pathogenesis Centre of Excellence, a collaboration between the Universities of Newcastle, Glasgow and Birmingham, aims to address the unmet needs of the 400,000 people who suffer from the crippling joint condition.. The three participating centres are committing an additional £4m in financial support over five years.. Rheumatoid arthritis is a serious, inflammatory, auto-immune condition that affects the joints and the bodys internal organs leading to chronic pain and fatigue. It occurs when the bodys immune system attacks itself. Although drug treatments have considerably improved in the past 20 years they are not effective in all patients.. Researchers at the new three-site centre, led at Newcastle University by Professor John Isaacs, professor of clinical rheumatology and director of the ...
Authors: Singarapu, Kiran Kumar; sukumaran, Dinesh; Parish, David; Chen, Chen; Kellie, Cunningham; Rong, Xiao; G V T, Swapna; Montelione, Gaetano; Szyperski, Thomas. Citation: Singarapu, Kiran Kumar; Xiao, Rong; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas. "NMR structure of protein Cgl2762 from Corynebacterium glutamicum implicated in DNA transposition reveals a helix-turn-helix motif attached to a flexibly disordered leucine zipper." Proteins 70, 1650-1654 (2008).. Assembly members: ...
Definition of Mus (genus) in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is Mus (genus)? Meaning of Mus (genus) as a finance term. What does Mus (genus) mean in finance?
Ur it si k te, e tenhle tygr m hodn zvl tn hlavu. Tuhle samici si pamatuji ji z pot p n p ed p ti lety. Jmenuje se Buttface a bu m vrozenou deformaci, nebo utrp la docela zav n zran n , ze kter ho se perfektn zotavila. ekal bych, e za 5 let vyroste v ce. T m p dem si k m, e p timetrov tyg i mus b t star , ne se obvykle uv d , ur it n kolik des tek let ...
Amavubi yabatarengeje imyaka 20 mu mupira wamaguru yashyizwe mu itsinda D mu mikino ya COSAFA yatumiwemo gusimbura Madagascar itazaboneka.
Source: Umuvugizi.com Ubu buhambya bwa Dr Rudasingwa, ikinyamakuru Umuvugizi gifitiye kopi, ni ubuhamya bukomeye ku muntu nka we wari mu basirikari bakuru (major) mu ngabo za FPR-Inkotanyi, akaba yaranagizwe umunyamabanga mukuru wishyaka rya FPR, nyuma gato yuko rifashe ubutegetsi muri nyakanga 1994. Ubu buhamya kandi Dr Rudasingwa yashyize ahagaragara uyu munsi, nta gushidikanya ko bwakoze mu bwonko Perezida Kagame kuko uyu, mu biganiro yakunze kugirana nabanyamakuru mpuzamahanga, yahakanaga yivuye inyuma ko ntaho ahuriye nurupfu rwa Perezida Habyarimana. Mu kiganiro cyitwa «Hard Talk» gihita kuri radiyo BBC mu rurimi rwicyongereza, ubwo umunyamakuru yamubazaga niba ari we koko wahanuye indege ya Perezida Habyarimana, aho kumusubiza ikibazo yari abajijwe, Kagame yahisemo kugikwepa, asubiza ko «Habyarimana yari yaramuhejeje i Shyanga imyaka mirongo itatu». Si Rudasingwa wenyine utanze ubuhamya nkubu, kuko mu mwaka w1998, umunyamakuru witwa Jean-Pierre Mugabe, wayoboraga «Le Tribun du ...
Ruben Muñoz is the author of this article in the Journal of Visualized Experiments: Подготовка и метаболических Assay 3-мерной сфероида Сопредседатель культур клеток поджелудочной железы и фибробластов
Dejte pozor, komu tyhle trubičky dáte ochutnat, protože pak na ně bude jezdit každé Vánoce. Ledaže byste mu prozradili recept...
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TABLE-US-00001 TABLE 1 Representative RNA molecules tested in Example 1 Duplex ID Sense strand sequence Antisense strand sequence A mC.mU.mC.mU.mU.mC.mG.mG.mA.mA.mA.mG.A. U. U. U. U. U. G. G. A. A. C. A. G. C. U. C. U. U. U. C. C. G. A. A. G. A. G U.mG.mU.mU.mC.mC.mA.mA.mA.mA.mA*dC*dC* (SEQ ID NO: 2) dT*dT*dC*dC*dC*dT*dG*dA*dA*dG (SEQ ID NO: 1) B mC.mU.mC.mU.mU.mC.mG.mG.mA.mA.mA.mG.m U. U. U. U. U. G. G. A. A. C. A. G. A.mC. U. G. U. C. U. U. U. C. C. G. A. A. G. A. G U.mU.mC.mC.mA.mA*mA*mA*mA*dC*dC*dT*dT (SEQ ID NO: 4) *dC*dC*dC*dT*dG*dA*dA*dG*dG*dT*dT*dC*dC *dT*dC*dC (SEQ ID NO: 3) C mG.mC.mA.mC.mU.mC.mU.mG.mA.mU.mU.mG.A. A. A. A. U. C. G. U. A. U. U. U. G. C. U. C. A. A. U. C. A. G. A. G. U. G. C A.mA.mA.mU.mA.mC.mG.mA.mU.mU.mU*dC*dC* (SEQ ID NO: 6) dT*dT*dC*dC*dC*dT*dG*dA*dA*dG (SEQ ID NO: 5) D mG.mC.mA.mC.mU.mC.mU.mG.mA.mU.mU.mG. A. A. A. A. U. C. G. U. A. U. U. U. G. C. U. C. A. A. U. C. A. G. A. G. U. G. C A.mA.mA.mU.mA.mC.mG.mA.mU.mU.mU*dC*dC* (SEQ ID NO: 8) ...
V jihomoravsk m Podlu pomalu nen obec, jej est by neh jil vlastn mu sk p veck sbor. Mu ci jsou podle etnograf unik tn fenom n. Sbory pr v pl nuj sv j festival.
Ubushakashatsi buheruka gukorwa bugaragaza ko imizabibu isa na mauve igira akamaro mu kurinda kanseri yamabere. Umutobe uva muri iyi mizabibu urwanya ibibyimba biza mu mabere akaba ariyo mvano nyamukuru ya kanseri yibere. Ntabwo nyamara bigarukira kuri kanseri yamabere gusa kuko no ku bundi bwoko bwose bwa kanseri gukoresha iyi mizabibu bifasha mu kuyirwanya. Ibi biterwa nuko mu mizabibu harimo resveratrol ikaba izwiho kubyimbura no kurwanya kanseri. Iyi resveratrol byumwihariko ni ingenzi mu kurinda kanseri yamara niyamabere. Si ukuzirinda gusa ahubwo, kuko inarwanya ko yakomeza gukura no gukwirakwira mu mubiri iyo yamaze kuwugeramo.. ...
SAMMENDRAG. Celler er kontinuerlig utsatt for påvirkninger som kan skade dets DNA. Dette inkluderer oksidative DNA-skader, som oppstår via normal oksidativ metabolisme, og fra forbindelser i miljøet. Slike DNA-skader kan forårsake mutasjoner. Cellene har imidlertid ervervet mekanismer for å reparere DNA-skader for å opprettholde arvematerialets integritet. En viktig mekanisme for å reparere oksidative DNA-skader er baseutkuttingsreparasjon (BER).. Arvematerialets integritet i testikkelceller er svært viktig da disse cellene bærer informasjonen nødvendig for videre generasjoner. Tidligere har avdelingen som denne hovedoppgaven er utført ved vist klare forskjeller i evnen til å reparere visse typer oksidative DNA-skader i testikkelceller mellom mennesker og gnagere. I motsetning til rotte og mus har menneskets testikkelceller liten eller ingen evne til å reparere oksiderte puriner. Gnagere er derfor muligens ikke ideell som modell for studier av oksiderende kjemikaliers virkninger på ...
Hi lifesavers! I need some assistance. I need a formula so I can solve the following: In a Poisson distribution where mu=4: a)What is the probability
Inima este muşchiul care pompeză continuu oxigen si sânge în tot corpul. Fiecare bătaie a inimii este stimulată de semnale electrice care trec prin mu...
Quote: Originally Posted by mus1mus Still depends on your Voltage levels. And if you have the old type with the heat pipes, pretty easy to get 4.4 on them. They are...
Natyra e Durrësit të lashtë me detin herë zulmëmadh e herë qetanak, rrethinat si ato kopshtet e varura shkallë- shkallë, kanë qenë dhe janë mrizi ku gjallojnë zanat e poezisë. Deti, deti me gjuhën e dallgëve, që herë shkërmoqet me zulme
FOLIKULSTIMULIRAJUCI HORMON (FSH) Podsti e nastajanje i sazrevanje folikula u ovarijumu ena, a ubrzava i lu enje estrogenih hormona. Kod mu karaca FSH hormon s
Výživný ATODERM Préventive 200ml od Biodermy. Ku každému nákupu vzorka zdarma a vernostné body. Expedujeme do 24 hod. + doprava zdarma.
I spent most of my teenage years feeling abandoned. Its sad to think about, but its just the way it was. You see, my stepmom adopted me when I...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Pierre Auguste Renoir (1841-1919)Richard Wagner1882Oil on canvasH. 53; W. 46 cmParis, Musée dOrsay© RMN-Grand Palais (Musée dOrsay) / Hervé Lewandowski ...
Chcete sv mu d t ti vysv tlit, jak se chovat k jin m n rodnostem? M te doma d t jin n rodnosti a chcete mu pomoci zvl dat p ek ky? P te n magaz n MF DNES Rodina DNES sestavil n kolik rad, co a jak ne kat d tem, aby vyr staly ve vz jemn toleranci.
References for Abcams Recombinant human PKC mu protein (ab60873). Please let us know if you have used this product in your publication
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N strahy na cest do Evropsk unie po sp n m referendu zdaleka nekon . Aby se 1. kv tna 2004 e t ob an stali tak ob any unie, mus zem splnit adu po adavk ze strany Bruselu. esk parlament ek je t ratifikace smlouvy o vstupu eska do EU.
Ruben Muñoz is the author of this article in the Journal of Visualized Experiments: Vorbereitung und metabolische Assay von 3-dimensionalen Sphäroid Ko-Kulturen von Pankreaskarzinom-Zellen und Fibroblasten
Pri bližšom pohľade zisťujeme, že odtieň vanilkového kvetu nie je zďaleka tak krotký, ako by sa mohlo zdať. Lesklé prevedenie mu dáva nečakanú hĺbku a silu. Napriek tomu nie je tento dekor dravcom, ktorý by vedľa seba nezniesol iné, jeho potenciál ku kombináciám je značný.
Your title is heartbreaking to see - the "never a mom" really got to me. I really really hope that your posts title does not stay true forever. It is so frustrating the lack of control and uncertainty about if/when you will become a mom too. And Im sure that frustration becomes heartbreaking as others close to you become moms twice over. You should definately not allow yourself to feel bad about what you are feeling - I would feel exactly the same way. Go ahead and feel your feelings and I will be here to empathize. ...
Amakuru ava mu biro vyumukuru wigihugu amenyesha ko abagize umurwi wigihugu ujejwe ibiganiro bizohuza abarundi (CNDI : Commission Nationale de Dialogue Interburundais) barahira kuri uwu wa gatanu.. Abo baheruka kugenwa kwitegeko ryumukuru wigihugu nomero 100/46 kugira bazoyobore ibiganiro bizohuza abarundi barahira imbere yumukuru wigihugu Petero NKURUNZIZA, mu gatondo ko kuri uwu wa gatanu, mu ntara ya Gitega.. Uwo murwi wigihugu ujejwe ibiganiro bizohuza abarundi ugizwe nabantu cumi na batanu, abagabo cumi hamwe nabagore batanu.. Umukuru wuwo murwi ni Musenyeri Justin NZOYISABA. ...
Infinitely better than any other Sloe Gin out there.Unlike the bulk of Sloe Gins out there today, Plymouth Sloe Gin is made from a excellent gin base and fresh sloe berries. - Read the full review ...
Your first hours as a new mom can take you through a spectrum of emotions. Read here to find out what to expect and how it feels to become a new mom.
Being a mom is a private journey, but the events that shape us as moms are often lived out loud on the national stage. Here, 72 milestones that have m
Siekiame užtikrinti, kad visi, pabėgę nuo gimtojoje šalyje patiriamo smurto, persekiojimo, karo ar stichinės nelaimės, turėtų teisę prašyti prieglobsčio ir surastų saugią užuovėją.. Nuo 1950 m.(??) sprendėme krizines situacijas įvairiuose žemynuose, teikdami gyvybiškai svarbią pagalbą pabėgėliams, prieglobsčio prašytojams, šalies viduje perkeltiems asmenis ir asmenims be pilietybės, kurių dauguma neturėjo į ką kreiptis pagalbos.. Milijonams savo namus palikti priverstų žmonių padedame išsigelbėti ir susikurti šviesesnę ateitį.. ...
v dsk policie zabavila p tadvacetilet mu mu i z m sta Bojsenburg bramborov d lo, kter postavil spolu s kamar dy. Z okna sv ho bytu j m st lel brambory do okol . D lo si vyrobil podle televizn ho po adu, kter ho inspiroval.
Ve dnech 7. - 9. 12. 2017 se konal v Olomouci 21. kongres o ateroskleróze pořádaný Českou společností pro aterosklerózu. V rámci kongresu byly hodnoceny nejlepší publikace a postery.
... is a disorder of the eye in which the retina separates from the layer underneath . Symptoms include an increase in the number of floaters , flashes of light , and worsening of the outer part of the visual field . This may be described as a curtain over part of the field of vision. In about 7% of cases both eyes are affected. Without treatment permanent loss of vision may occur. The mechanism most commonly involves a break in the retina that then allows the fluid in the eye to get behind the retina. A break in the retina can occur from a posterior vitreous detachment , injury to the eye, or inflammation of the eye. Other risk factors include being short sighted and previous cataract surgery. Retinal detachments also rarely occur due to a choroidal tumor . Diagnosis is by either looking at the back of the eye with an ophthalmoscope or by ultrasound . In those with a retinal tear, efforts to prevent it becoming a detachment include cryotherapy using a cold probe or
Ku munsi wumuganura na mbere gato ubwo habaga igitaramo cyi Nyanza Twataramye, icyumba cyo gucumbikamo mu nzu zishinzwe gucumbikira abagenzi mu Karere ka Nyanza cyishyurwaga ibihumbi icumi kuzamura mu gihe mu minsi isanzwe hari naho kiboneka ku bihumbi bitandatu.. Uko kuzamurirwa ibiciro kwibyumba kwajyanye no kuba iyanga kuko bamwe barabibonye, abandi babura aho bifuzaga, ariko babona ahandi bacumbika.. Koko rero, kuwa mbere Kanama 2019 nibwo i Nyanza haraye abashyitsi benshi baturutse mu bice bitandukanye byIgihugu. Abayobozi mu nzego za Leta, Abikorera, abahanzi nabandi bari benshi cyane.. Bataramiwe nabahanzi mu byiciro bitandukanye, mu Rukari, ahari Ingoro yumurage igaragaza amateka yAbami. Mu Rukari hakunze kubera iki gitaramo, ku buryo abashyitsi baboneraho gusura iyo ngoro yumurage.. Nyuma yicyo gitaramo, bwakeye ba bashyitsi baharaye biyongeraho abandi benshi kandi bicyubahiro, nabo baje mu munsi mukuru wUmuganura. Ibirori byabereye kuri Stade ya Nyanza.. Ibi bikorwa ...
Bacteriophages (phages) are probably the most abundant entities in nature, often exceeding bacterial densities by an order of magnitude. As viral predators
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ MS2 Bacteriophage \ GEN0810066 for more molecular products just contact us
RPA172Mu02, Recombinant Platelet Factor 4 (PF4), 血小板因子4(PF4)重组蛋白, CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Compare Samsung UE65MU6120 prices among hundreds of retailers, read reviews for Samsung TVs. Use PriceRunners easy TVs price comparison tools to help you find the best value Samsung UE65MU6120 TVs online.
2212556DNAOryzias latipes 1atggagaaaa aaaggtcaaa gccatctggt gcccaattta gaaagaaaag aaaagaagaa 60gaggagaaaa gagataaaga aaagggggca cttctaagat attttggatc gtctaccact 120gctcaagatg agacatctac ctccctgcca gctatctcat cagccacagt cacagtctca 180ccccctcagg atgagctacc atctacatcc tctgctactc atgtagttcc acagttgtta 240cctgagcaaa gttttgatag tgaggctgaa gacgttgttc catctacgtc tacccagctt 300gagacttcag aaatgcctgg tgatgaaacc ccactgaccc cgactgctga ggaccagcct 360ctaccaactg accctgcaaa gtggccctca cctctgactg acaggatacg gatggagctg 420gttcgaagag gaccaagtag cataccacct gactttgttt tcccaagaaa tgacagtgat 480gggagaagtt gtcatcacca ctatttcagg aagacactag taagtggtga aaaaatagca 540agaacttggt tgatgtattc aaaagtgaag aacagcctct tttgcttttg ttgcaaattg 600ttttccaaca aaaacattaa tttaacaact tctggtacag caaactggaa acatgcaagc 660acatacctca cagcacacga aaaaagccca gaacacctca attgtatgaa agcatggaag 720gaactgtcag ggaggatcag aagtgggaaa acaattgata agcaggagat ggcacttctg 780gaagaggagc gggtgagatg gagagcagtg ctaacccgtc tcattgctat tgtgcagtca 840ctggcagttc ...
In Be the Mom, Tracey Eyster describes seven mom traps that moms often fall into, and step-by-step, she provides advice for avoiding and escaping these traps in your own life. Traceys delightful, down-to-earth style will make you smile, equip you with the tools you need, and remind you what being the mom is all about. Be the Mom: Overcome Attitude Traps and Enjoy Your Kids (9781589976849) by Tracey Eyster
Dieta se nemus dr et jen kv li t hl linii, lov k se pro ni m e rozhodnout i kv li palmov mu oleji, indon sk m prales m a orangutan m. Vy adit nebo alespo omezit palmov olej ve sv spot eb znamen dobrovoln vzd t se nejedn sv obl ben sladk i slan pochoutky a pe liv zkoumat slo en ka d ho jednotliv ho v robku v obchod .
Ilolelini vino ya Nte Yakwe Yeova yakaomba-yaaya impendwa ku ma 8 milyoni kufuma mu mitundu ipusane-pusane mu mpanga ukucila pali 230, nupya yalemenkana mu kupepa Leza nu kuomba imilimo.
Vsetko o hifi - SK a CZ diskusie, stereo, konstrukcie, zapojenia, audio, cd, dac, highend, gramofon, sluchatka, hudba, akustika, reproduktory, zosilnovace.
Intímny sprchový gél na hygienu žien vo fertilnom veku, vhodný po športovaní, počas menštruácie, pri gynekologických problémoch a zápachu intímnych partií
Roberto Anaya-Alaminos, José I. Muñoz-Ávila, María C. González-Gallardo, Eduardo R. Mora-Horna, José L. García-Serrano, María V. Ramírez-Garrido Eur J Ophthalmol 2014; 24(5): 808 - 810 ...
Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase--correlation of initial velocity, bound intermediate, and oxygen exchange measurements with an alternating three-site model ...
Usually bacteriophages lyse their hosts following infection, however a few so-called "temperate" phage undergo lysogeny. In lysogeny, the bacteriophage integrates its genome into that of its host. The phage, then, is replicated each time the bacterial cell divides. In the lysogenic state, the bacteriophage can have considerable influence over host physiology ...
PKC mu小鼠多克隆抗体(ab21938)可与果蝇样本反应并经WB实验严格验证,被4篇文献引用。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Autoimunitn onemocn n centr ln ho nervov ho syst mu (CNS) mohou b t projevem syst mov i org nov specifick autoimunity, ale existuje i cel ada syndrom s prim rn m i dominuj c m posti en m CNS. Klinicky se mohou projevovat r zn mi p ...
Looking for 5.11 TACTICAL Stryke Pant,Size 34,Charcoal (22MU68)? Graingers got your back. Price:$86.25. Easy ordering & convenient delivery. Log-in or register for your pricing.
This poster is on display at my moms place of work - the quote on it is from a patient For as long as I can remember, my mom has been a nurse. In reality, shes been a nurse for...
The number of successful propagations/isolations of soil-borne bacteriophages is small in comparison to the number of bacteriophages observed by microscopy (great plaque count anomaly). As one...

*Mobile genetic elements

Bacteriophage elements, like ;Mu, which integrates randomly into the genome by transduction. Group I and II introns are a ... Classification of mobile genetic elements Horizontal gene transfer Virulence factors Mu, X.; Ahmad, S.; Hur, S. Endogenous ...

*Tn3 transposon

Shapiro, James (April 1979). "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ...

*DNA

Nature 175 (4451) 336-337 Allet B, Bukhari AI (1975) Analysis of bacteriophage Mu and λ-Mu hybrid DNAs by specific ... However, in several bacteriophages, Bacillus subtilis bacteriophages PBS1 and PBS2 and Yersinia bacteriophage piR1-37, thymine ... In 1976 a bacteriophage - S-2L - which infects species of the genus Synechocystis was found to have all the adenosine bases ... Casella E, Markewych O, Dosmar M, Heman W (1978) Production and expression of dTMP-enriched DNA of bacteriophage SP15. J ...

*Fis

Purification and properties of the Escherichia coli host factor required for inversion of the G segment in bacteriophage Mu. J ... Fis was first discovered for its role in stimulating Gin catalyzed inversion of the G segment of phage Mu genome. Fis was ... In addition to bringing about overall downregulation of the Mu genome, it also ensures silencing of the advantageous but ... as the factor for inversion stimulation of the homologous Hin and Gin site-specific DNA recombinases of Salmonella and phage Mu ...

*List of MeSH codes (B04)

... bacteriophage mu MeSH B04.123.150.500.300 --- bacteriophage p1 MeSH B04.123.150.500.305 --- bacteriophage p2 MeSH B04.123. ... bacteriophage m13 MeSH B04.123.205.260 --- bacteriophage mu MeSH B04.123.205.280 --- bacteriophage n4 MeSH B04.123.205.300 --- ... bacteriophage mu MeSH B04.280.090.500.300 --- bacteriophage p1 MeSH B04.280.090.500.305 --- bacteriophage p2 MeSH B04.280. ... bacteriophage phi x 174 MeSH B04.123.660.535 --- bacteriophage pf1 MeSH B04.123.660.550 --- bacteriophage phi 6 MeSH B04.123. ...

*Ronald Plasterk

"Inversion of the G segment of bacteriophage Mu: analysis of a genetic switch". His study focused on transposon sequences in DNA ...

*Replicative transposition

Shapiro, J. A. (1979), "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ... "Transposition of phage Mu DNA", in Craig, N. L.; Craigie, R.; Gellert, M.; Lambowitz, A. M., Mobile DNA II, American Society ...

*Bacteriophage Mu

... , also known as mu phage or mu bacteriophage, is a mulikevirus (the first of its kind to be identified) of the ... create a crystal structure of the Mu bacteriophage transposome, allowing for a detailed understanding of the process Mu ... doi:10.1016/0022-2836(75)90183-7. Shapiro JA (1979). "Molecular model for the transposition and replication of bacteriophage Mu ... PMC 383507 . Kiyoshi Mizuuchi (1983). "In vitro transposition of bacteriophage Mu: a biochemical approach to a novel ...

*Bacteriophage P2

The high level of similarities in the tail fiber genes of phage P2, P1, Mu, λ, K3 and T2, which belong to different families, ... The P2-like bacteriophages. In R. Calendar (ed.), The bacteriophages. Oxford Press, Oxford, 2005: p. 365-390 Lindahl, G., ... Bacteriophage P2 was first isolated by G. Bertani from the Lisbonne and Carrère strain of E. coli in 1951. Since that time, a ... Bacteriophage P2 is a temperate phage that infects E. coli. It is a tailed virus with a contractile sheath and is thus ...

*Category:Low-importance virus articles

Talk:Bacteriophage Mu. *Talk:Bacteriophage P2. *Talk:Bacteriophage PBC1. *Talk:Bacteriophage Qβ ...

*Bacteriophage

T4 phage, Mu, PBSX, P1Puna-like, P2, I3, Bcep 1, Bcep 43, Bcep 78 ... Bacteriophages are among the most common and diverse entities in the biosphere.[1] Bacteriophages are ubiquitous viruses, found ... A bacteriophage (/bækˈtɪərioʊfeɪdʒ/), also known informally as a phage (/feɪdʒ/), is a virus that infects and replicates within ... 2×108 bacteriophages per mL.[47] Bacteriophages are thought to extensively contribute to horizontal gene transfer in natural ...

*P1 phage

"Bacteriophage P1", in Richard Calendar (ed.), The Bacteriophages, Oxford University Press, p. 350, ISBN 0195148509 CS1 maint: ... This system has close sequence homologies to recombinational systems in the tail fibers of unrelated phages like the mu phage ... The genome of P1 encodes 112 proteins and 5 untranslated genes and is this about twice the size of bacteriophage lambda. The ... Sandmeier, H.; S. Iida; W. Arber (1992-06-01). "DNA Inversion Regions Min of Plasmid p15B and Cin of Bacteriophage P1: ...

*Listeria monocytogenes

A bacteriophage, Listeria phage P100, has been proposed as food additive to control L. monocytogenes. Bacteriophage treatments ... Ramaswamy V; Cresence VM; Rejitha JS; Lekshmi MU; Dharsana KS; Prasad SP; Vijila HM. (February 2007). "Listeria - review of ... The U.S. Food and Drug Administration (FDA) approved a cocktail of six bacteriophages from Intralytix, and a one-type phage ... Carlton, RM; Noordman, WH; Biswas, B; De Meester, ED; Loessner, MJ (2005). "Bacteriophage P100 for control of Listeria ...

*Chemostat

... mu ={\frac {\ln 2}{t_{d}}}} Therefore, the doubling time td becomes a function of dilution rate D in steady state: t d = ln ⁡ 2 ... specific types of bacterial mutants in culture such as auxotrophs or those that are resistant to antibiotics or bacteriophages ... mu _{\max }{S \over K_{S}+S},} where S is the substrate or nutrient concentration in the chemostat and KS is the half- ...

*Membrane technology

The retention of bacteriophage and bacteria, the so-called "bacteriachallenge test", can also provide information about the ... mu }}\ A\left({\frac {1}{R_{m}+R}}\right)} where Vp and Q are the volume of the permeate and its volumetric flow rate ...

*Myoviridae

The Myoviridae is a family of bacteriophages in the order Caudovirales. Bacteria and archaea serve as natural hosts. There are ... Enterobacteria phage Mu Genus: Pbunalikevirus Burkholderia phage BcepF1 Pseudomonas phage 14-1 Pseudomonas phage F8 Pseudomonas ... "Bacteriophage vB_EcoM_FV3: A new member of "rV5-like viruses"". Archives of Virology. 157 (12): 2431-5. doi:10.1007/s00705-012- ...

*DNA virus

The largest bacteriophage known is Klebsiella Phage vB_KleM-RaK2 which has a genome of 346 kilobases. A recently proposed clade ... Mu-like transposition or the plasmid-like partitioning of phage N15. A putative course of evolution of these phages has been ... Several dsDNA bacteriophages and the herpesviruses encode a powerful ATP driven DNA translocating machine that encapsidates a ... To a lesser extent this gene is also found in T4-like bacteriophages suggesting a common ancestor for these two groups of ...

*Antibiotic use in livestock

Bacteriophages are able to infect most bacteria and are easily found in most environments colonized by bacteria, and have been ... Mu, Quanhua; Li, Jin; Sun, Yingxue; Mao, Daqing; Wang, Qing; Yi, Luo (5 December 2014). "Occurrence of sulfonamide-, ... Joerger R.D. (2003). "Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages". Poultry Science. ... antimicrobial peptides and bacteriophages in the control of bacterial infections.[133] While further research is needed in this ...

*Protein engineering

These aforementioned techniques all require the design of mini-Mu transposons. Thermo scientific manufactures kits for the ... This method utilizes a bacteriophage with a modified life cycle to transfer evolving genes from host to host. The phage's life ...

*List of geneticists

Nobel Prize for bacteriophage genetics Jay Lush (1896-1982), American animal geneticist who pioneered modern scientific animal ... notable contributions to the study of phage Mu T. C. Hsu (1917-2003), distinguished Chinese-American cell biologist, geneticist ...

*Index of genetics articles

Mosaicism Mouse model mRNA mtDNA Mu particle Mu phage Mullerian mimicry Multifactorial Multihybrid Multimeric structure ... mutant Axoneme B form DNA Bacillus Back mutation Backcross Bacteria Bacterial conjugation Bacterial lawn Bacteriophage Balbiani ...

*Gaussian network model

... mu ,\Sigma )={\frac {1}{\sqrt {(2\pi )^{N},\Sigma ,}}}\exp \left\{-{\frac {1}{2}}(x-\mu )^{T}\Sigma ^{-1}(x-\mu )\right\}} ( 2 ... Rader, AJ; Vlad, Daniel; Bahar, Ivet (2005). "Maturation Dynamics of Bacteriophage HK97 Capsid". Structure (Camb). 13: 413-21. ...

*Quantum dot

displaystyle \Delta \,V={\Delta \,\mu \, \over e}={\mu (N+\Delta \,N)-\mu (N) \over e}}. may be applied to a quantum dot with ... Genetically engineered M13 bacteriophage viruses allow preparation of quantum dot biocomposite structures.[23] It had ... displaystyle C(N)={e^{2} \over \mu (N+1)-\mu (N)}={e^{2} \over I(N)-A(N)}}. is the "quantum capacitance" of a quantum dot, ... mu a^{2}}}\\E_{\textrm {exciton}}&=-{\frac {1}{\epsilon _{r}^{2}}}{\frac {\mu }{m_{e}}}R_{y}=-R_{y}^{*}\\E&=E_{\textrm {bandgap ...

*Anthrax

Mu Gao (27 April 2006). "Molecular Basis for Anthrax Intoxication". University of Illinois at Urbana-Champaign. Archived from ... Confirmatory testing to identify B. anthracis includes gamma bacteriophage testing, indirect hemagglutination, and enzyme- ...

*Taxonomic list of viruses

Enterobacteria phage Mu Genus: Pbunalikevirus Burkholderia phage BcepF1 Pseudomonas phage 14-1 Pseudomonas phage F8 Pseudomonas ... Thermus thermophilus bacteriophage p23-77 Thermus thermophilus phagein in93 Family: Tectiviridae Genus: Tectivirus Bacillus ...

*Heliks-zavoj-heliks

Anderson WF, Ohlendorf DH, Takeda Y, Matthews BW (1981). "Structure of the cro repressor from bacteriophage lambda and its ... HTH_Tnp_Mu_2 Pfam PF09039 Pfam klan CL0123 InterPro IPR015126 Dostupne PDB strukture: ...

*Membrane technology

displaystyle {\frac {dV_{p}}{dt}}=Q={\frac {\Delta p}{\mu }}\ A\left({\frac {1}{R_{m}+R}}\right)}. ... The retention of bacteriophage and bacteria, the so-called "bacteriachallenge test", can also provide information about the ...

*Membrane technology

displaystyle {\frac {dV_{p}}{dt}}=Q={\frac {\Delta p}{\mu }}\ A\left({\frac {1}{R_{m}+R}}\right)}. ... The retention of bacteriophage and bacteria, the so-called "bacteriachallenge test", can also provide information about the ...

Bacteriophage Mu, transposase (IPR004189) | InterPro | EMBL-EBIBacteriophage Mu, transposase (IPR004189) | InterPro | EMBL-EBI

Bacteriophage Mu, transposase (IPR004189). Short name: Phage_Mu_transposase Overlapping homologous superfamilies *Ribonuclease ... Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. ... This transposase is essential for integration, replication-transposition and excision of Bacteriophage Mu DNA. Transposition ... The crystal structure of the core domain of Mu transposase, MuA, has been determined. The first of two subdomains contains the ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR004189

Bacteriophage Mu - WikipediaBacteriophage Mu - Wikipedia

Bacteriophage Mu, also known as mu phage or mu bacteriophage, is a mulikevirus (the first of its kind to be identified) of the ... create a crystal structure of the Mu bacteriophage transposome, allowing for a detailed understanding of the process Mu ... doi:10.1016/0022-2836(75)90183-7. Shapiro JA (1979). "Molecular model for the transposition and replication of bacteriophage Mu ... PMC 383507 . Kiyoshi Mizuuchi (1983). "In vitro transposition of bacteriophage Mu: a biochemical approach to a novel ...
more infohttps://en.wikipedia.org/wiki/Bacteriophage_Mu

RCSB PDB 









- 1BCM: BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT Macromolecule...RCSB PDB - 1BCM: BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT Macromolecule...

Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. ... mu transposase, C-terminal domain mu transposase, C-terminal domain mu transposase, C-terminal domain Bacteriophage mu [TaxId: ... mu transposase, C-terminal domain mu transposase, C-terminal domain mu transposase, C-terminal domain Bacteriophage mu [TaxId: ... mu transposase, core domain mu transposase, core domain Bacteriophage mu [TaxId: 10677] ...
more infohttp://www.rcsb.org/pdb/explore/derivedData.do?structureId=1BCM

The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku | EMBO ReportsThe Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku | EMBO Reports

Ec, Escherichia coli O157:H7; Hi, Haemophilus influenzae; Mu, bacteriophage Mu; Nm, Neisseria meningitidis; St, Salmonella ... The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku. Fabrizio dAdda di Fagagna, Geoffrey R Weller, Aidan J ... Akroyd, J. & Symonds, N. (1986) Localization of the gam gene of bacteriophage mu and characterisation of the gene product. Gene ... Mu bacteriophage inserts its DNA into the genome of host bacteria and is used as a model for DNA transposition events in other ...
more infohttp://embor.embopress.org/content/4/1/47

Role of bacteriophage Mu C protein in activation of the mom gene promoter. - Semantic ScholarRole of bacteriophage Mu C protein in activation of the mom gene promoter. - Semantic Scholar

The phage Mu C gene product is a specific activator of Mu late gene transcription, including activation of the mom operon. ... Regulation of bacteriophage Mu transposition. *Ariane Toussaint, M J Gama, Jamal E Laachouch, Geneviève Maenhaut-Michel, Amina ... Translation of the bacteriophage Mu mom gene is positively regulated by the phage com gene product. *F Gregory Wulczyn, Michael ... Mutational analysis of a C-dependent late promoter of bacteriophage Mu.. *Liu Wei Chiang, Martha M. Howe ...
more infohttps://www.semanticscholar.org/paper/Role-of-bacteriophage-Mu-C-protein-in-activation-of-B%C3%B6lker-Wulczyn/2b0d7bf8ab899df6e4d4da09ed6cc23db45b199c

Mg2+ Mediated Sequence-Specific Binding of Transcriptional Activator Protein C of Bacteriophage Mu to DNA  - ePrints@IIScMg2+ Mediated Sequence-Specific Binding of Transcriptional Activator Protein C of Bacteriophage Mu to DNA - [email protected]

The contributions from the secondary structure of the transcriptional activator protein C of bacteriophage Mu to its specific ... Mg2+ Mediated Sequence-Specific Binding of Transcriptional Activator Protein C of Bacteriophage Mu to DNA ... Mg2+ Mediated Sequence-Specific Binding of Transcriptional Activator Protein C of Bacteriophage Mu to DNA. In: Biochemistry, 37 ...
more infohttp://eprints.iisc.ernet.in/2377/

RCSB PDB 









for 1BCORCSB PDB for 1BCO

Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. ... BACTERIOPHAGE MU TRANSPOSASE A 327 Escherichia virus mu EC#: 3.1.22 IUBMB 6.5.1 IUBMB Gene Name(s): A Mup03 ...
more infohttp://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1BCO

Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis - pdf descargarGenomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis - pdf descargar

... a Mu-like bacteriophage infecting Haemophilus parasuis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos ... Mu-like bacteriophages are related phyologenetically to enterobacteriophage Mu and are thought to carry virulence genes or to ... Previously, a Mu-like bacteriophage portal gene was detected in a virulent swine isolate of H. parasuis by nested polymerase ... DNA sequencing revealed fifty five open reading frames, including twenty five homologs to Mu-like bacteriophage proteins: Nlp, ...
more infohttp://libros.duhnnae.com/2017/jul5/149918382713-Genomic-and-proteomic-characterization-of-SuMu-a-Mu-like-bacteriophage-infecting-Haemophilus-parasuis.php

Bacteriophages have been found to have new uses-making car fuel! | MU Science BlogBacteriophages have been found to have new uses-making car fuel! | MU Science Blog

The M13 bacteriophage is often used in molecular genetics work as a cloning vector. The phage contains a single strand circular ... Bacteriophages have been found to have new uses-making car fuel!. *Is it a good or bad time for students who dream of going to ... Bacteriophages have been found to have new uses-making car fuel! April 29, 2010 Posted by Jill in Biology, Chemistry, ... The M13 bacteriophage is often used in molecular genetics work as a cloning vector. The phage contains a single strand circular ...
more infohttps://marianuniversityscienceblog.wordpress.com/2010/04/29/bacteriophages-have-been-found-to-have-new-uses-making-car-fuel/

Complete Genomic Sequence of SfV, a Serotype-Converting Temperate Bacteriophage of Shigella flexneri | Journal of BacteriologyComplete Genomic Sequence of SfV, a Serotype-Converting Temperate Bacteriophage of Shigella flexneri | Journal of Bacteriology

The tail assembly and structural genes of SfV show homology to those of phage Mu and Mu-like prophages in the genome of ... The homology to phage Mu is consistent with the fact that SfV is in the same morphology group as Mu (Allison et al., submitted ... Bacteriophages, Transposons, and Plasmids. Complete Genomic Sequence of SfV, a Serotype-Converting Temperate Bacteriophage of ... Strains, phage and media.Bacteriophage SfV was originally induced from S. flexneri EW595/52 (27). Bacteriophage stocks were ...
more infohttps://jb.asm.org/content/184/7/1974?ijkey=0857bd4cacfaaa3cba09fa11b5c9118fbf395cc2&keytype2=tf_ipsecsha

Toussaint A[au] - PubMed - NCBIToussaint A[au] - PubMed - NCBI

My life with Mu.. Toussaint A.. Bacteriophage. 2015 Apr 28;5(2):e1034336. eCollection 2015 Apr-Jun. No abstract available. ... Transposable Mu-like phages in Firmicutes: new instances of divergence generating retroelements. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Toussaint+A%5Bau%5D&dispmax=50

The coexistence of Escherichia coli serotype O157:H7 and its specific bacteriophage in continuous culture.  - PubMed - NCBIThe coexistence of Escherichia coli serotype O157:H7 and its specific bacteriophage in continuous culture. - PubMed - NCBI

15% of the Mu(L) population was completely resistant to PP01 infection. Mu(L) also co-existed with bacteriophages unrelated to ... we found that clonal Mu(L) cultures were heterogeneous in their ability to bind bacteriophage. ... We describe a new strain of Escherichia coli O157:H7, named Mu(L), which stably co-exists with the O157:H7-specific lytic ... The binding rate of PP01 to the cell surface was diminished 8.5-fold in Mu(L). By observation of the binding of fluorescently ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15598529?dopt=Abstract

DNA nucleotidylexotransferase (TdT) / DNA-directed DNA/RNA polymerase mu (IPR001726) | InterPro | EMBL-EBIDNA nucleotidylexotransferase (TdT) / DNA-directed DNA/RNA polymerase mu (IPR001726) | InterPro | EMBL-EBI

Bacteriophage PRD1 DNA polymerase: evolution of DNA polymerases.. Proc. Natl. Acad. Sci. U.S.A. 84 8287-91 1987 ... DNA nucleotidylexotransferase (TdT) / DNA-directed DNA/RNA polymerase mu (IPR001726). Short name: TdT/Mu ... Involvement of DNA polymerase mu in the repair of a specific subset of DNA double-strand breaks in mammalian cells.. Nucleic ... Polymerase mu is a DNA-directed DNA/RNA polymerase.. Mol. Cell. Biol. 23 2309-15 2003 ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR001726

Coevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture | Applied and Environmental MicrobiologyCoevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture | Applied and Environmental Microbiology

a) WT; (b) MuH mutant. The culture was incubated at 37°C and infected with bacteriophage PP01 (closed circles) at an MOI of 2 ... Coevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture. Katsunori Mizoguchi, Masatomo Morita, ... The Mu strains were highly mucoid colonies. Wt indicates WT E. coli O157:H7. The dilution rate from which a given strain was ... Samples of a continuous culture of E. coli O157:H7 and bacteriophage PP01 were taken at the indicated times, and the ...
more infohttps://aem.asm.org/content/69/1/170/figures-only

Proteins Involved in DNA Replication | Ulrich Huebscher | SpringerProteins Involved in DNA Replication | Ulrich Huebscher | Springer

Replication of Bacteriophage Mu and its Mini-Mu Derivatives. Resibois, Anne (et al.) ... Gene A Protein of Bacteriophage ΦX174 is a Highly Specific Single-Strand Nuclease and Binds Via a Tyrosyl Residue to DNA After ... Initiation of DNA Synthesis on Single-Stranded DNA Templates in Vitro Promoted by the Bacteriophage λ O and P Replication ... The Origin of DNA Replication of Bacteriophage fl and its Interaction with the Phage Gene II Protein ...
more infohttps://www.springer.com/us/book/9781468487329?wt_mc=

Buckwalter Lab Publications | Buckwalter Lab | Stanford MedicineBuckwalter Lab Publications | Buckwalter Lab | Stanford Medicine

Bacteriophage Mu sites required for transposition immunity. Darzins A, Kent NE, Buckwalter MS, Casadaban MJ. Proc Natl Acad Sci ...
more infohttp://med.stanford.edu/buckwalter-lab/publications.html

Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans, Escherichia coli, andPseudomonas aeruginosa |...Prevalence of Broad-Host-Range Lytic Bacteriophages of Sphaerotilus natans, Escherichia coli, andPseudomonas aeruginosa |...

1988) Phage Mu. in The bacteriophages, ed Callendar R. (Plenum Press, New York, N.Y), 1:193-234. ... 1988) Bacteriophage P1. in The bacteriophages, ed Callendar R. (Plenum Press, New York, N.Y), 1:291-438. ... Of these broad-host-range phages, P1 and Mu are the best studied. Bacteriophage P1 is a generalized transducing virus capable ... Bacteria and bacteriophages.Bacterial strains used for bacteriophage propagation and titration were E. coli AB1157 (5), P. ...
more infohttps://aem.asm.org/content/64/2/575

CiteSeerX - Citation Query Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as...CiteSeerX - Citation Query Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as...

Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as large as 100 kilobases pairs ... DNA, similar to that of phage Mu (reviewed in reference 268). Infective particles of P1 contain cyclically permuted, linear, ... The genome of bacteriophage P1 by Malgorzata B. Lobocka, Debra J. Rose, Guy Plunkett Iii, Marek Rusin, Arkadiusz Samojedny, ... Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as large as 100 kilobases pairs ...
more infohttp://citeseerx.ist.psu.edu/showciting?cid=1091564

Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of...Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of...

"Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of ... Genomic Sequences of Bacteriophages HK97 and HK022: Pervasive Genetic Mosaicism in the Lambdoid Bacteriophages ... Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains. Krylov, V.; Miller, S ... Ambivalent bacteriophages were found in species other than T-even phages and were similar in morphotype to lambdoid and other E ...
more infohttps://www.deepdyve.com/lp/springer_journal/ambivalent-bacteriophages-of-different-species-active-on-escherichia-DBbzQl28SV

DSpace@MIT: 
                Browsing Chemistry - Ph.D. / Sc.D. by Title[email protected]: Browsing Chemistry - Ph.D. / Sc.D. by Title

Organization and dynamics of the bacteriophage Mu transpososome  Williams, Tanya L. (Tanya Lynn), 1970- (Massachusetts ... Transposition of a linear mobile genetic element, such as the bacteriophage Mu, requires a set of spatially and temporally ...
more infohttp://dspace.mit.edu/handle/1721.1/7646/browse?rpp=20&order=ASC&sort_by=1&etal=-1&type=title&starts_with=O

Science Clips - Volume 12, Issue 23, July 14, 2020Science Clips - Volume 12, Issue 23, July 14, 2020

Whitworth C, Mu Y, Houston H, Martinez-Smith M, Noble-Wang J, Coulliette-Salmond A, Rose L.. Appl Environ Microbiol. 2020 Jun ... The bacteriophage Phi 6 has a phospholipid envelope and is commonly used in environmental studies as a surrogate for human ... Persistence of bacteriophage Phi 6 on porous and non-porous surfaces; potential for use as Ebola or coronavirus surrogate ...
more infohttps://www.cdc.gov/library/sciclips/issues/index.html

Diversity of Mycobacterium species from marine sponges and their sensitivity to antagonism by sponge-derived rifamycin...Diversity of Mycobacterium species from marine sponges and their sensitivity to antagonism by sponge-derived rifamycin...

Previous article in issue: Identification of the J and K genes in the bacteriophage Mu genome sequence Previous article in ... Identification of the J and K genes in the bacteriophage Mu genome sequence ...
more infohttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2010.02118.x/full?globalMessage=0

Tn3 transposon - WikipediaTn3 transposon - Wikipedia

Shapiro, James (April 1979). "Molecular model for the transposition and replication of bacteriophage Mu and other transposable ...
more infohttps://en.wikipedia.org/wiki/Tn3_transposon

Caffeic acid regulates LPS-induced NF-κB activation through NIK/IKK and c-Src/ERK signaling pathways in endothelial cells |...Caffeic acid regulates LPS-induced NF-κB activation through NIK/IKK and c-Src/ERK signaling pathways in endothelial cells |...

Lin, X., Y. Mu, E.T. Cunningham Jr, K.B. Marcu, R. Geleziunas, and W.C. Greene. 1998. Molecular determinants of NF-kappaB- ... Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.Google Scholar ... Chen, L.F., S.A. Williams, Y. Mu, H. Nakano, J.M. Duerr, L. Buckbinder, and W.C. Greene. 2005. NF-kappaB RelA phosphorylation ... Chen, L.F., Y. Mu, and W.C. Greene. 2002. Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF- ...
more infohttps://link.springer.com/article/10.1007%2Fs12272-013-0211-6

DSpace@MIT: 
                Dynamics of protein-DNA interactions in a Mu transpososome[email protected]: Dynamics of protein-DNA interactions in a Mu transpososome

... the genome of bacteriophage Mu. The first chapter provides an overview of Mu transposition, with special emphasis on the ... This process is required for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic ... Alternative Title: Dynamics of protein-Deoxyribonucleic acid interactions in a Bacteriophage mu transpososome ... Dynamics of protein-DNA interactions in a Mu transpososome. Research and Teaching Output of the MIT Community. ...
more infohttp://dspace.mit.edu/handle/1721.1/29918
  • Twenty protein homologs to bacteriophage proteins, including 15 structural proteins, one lysogeny-related and one lysis-related protein, and three DNA replication proteins were identified by mass spectrometry. (duhnnae.com)
  • Fuller and Rice conclude that if other proteins were to pre-bend or otherwise make the DNA more flexible, this would tell the DNA-joining enzyme where to insert, which helps explain the roles of known targeting proteins for Mu and HIV. (elifesciences.org)
  • We propose that these two proteins, which we have renamed LptF and LptG, respectively, are the missing transmembrane components of the ABC transporter that, together with LptB, functions to extract LPS from the IM en route to the OM. (pnas.org)
  • The present study was intended to identify all known members of the dystrophin superfamily and their associated proteins expressed in Müller glial cells (MGC). (arvojournals.org)
  • An immunocytochemical localization of the proteins was also performed on enzymatically dissociated Müller cells from adult rat retinas. (arvojournals.org)
  • In morphologically preserved differentiated Müller cells, Dp71f was localized in clusters, utrophin was diffusely distributed in the cytoplasm, and dystrophin-associated proteins (DAPs) were membrane-bound. (arvojournals.org)
  • Surprisingly, systematic studies of broad-host-range interactions of bacteriophages and of the relative frequencies with which such bacteriophages may be isolated are lacking. (asm.org)
  • Obtaining a high proportion of independent Em-Mu disruptions in icaADBC demonstrated both the importance of PIA for biofilm formation in this clinical strain and the strong validation of the screening procedure that concomitantly uncovered additional mutants. (asm.org)
  • Genetic analysis of the low calcium response in Yersinia pestis mu d1(Ap lac) insertion mutants. (pubmedcentralcanada.ca)
  • Morphogenetic structures present in lysates of amber mutants of bacteriophage Mu. (antibody-antibodies.com)
  • Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. (asm.org)
  • This entry represents terminal deoxynucleotidyl-transferase (TdT) and the DNA-directed DNA/RNA polymerase mu. (ebi.ac.uk)
  • Bacteriophage PRD1 DNA polymerase: evolution of DNA polymerases. (ebi.ac.uk)
  • Involvement of DNA polymerase mu in the repair of a specific subset of DNA double-strand breaks in mammalian cells. (ebi.ac.uk)
  • bacteriophage Sf6 encodes an acetyltransferase and confers conversion to serotype 3b ( 10 , 49 ). (asm.org)
  • 15% of the Mu(L) population was completely resistant to PP01 infection. (nih.gov)
  • Studies of bacteriophage infection have revealed that the process is initiated when the virion interacts with host cell surface receptor molecules ( 14 ). (asm.org)
  • However, the latent period, burst size, and growth rate of Mu(L) were the same as in a PP01-susceptible strain. (nih.gov)
  • Mu(L) also co-existed with bacteriophages unrelated to PP01. (nih.gov)
  • The culture was incubated at 37°C and infected with bacteriophage PP01 (closed circles) at an MOI of 2 at the time indicated by the arrow. (asm.org)
  • The bacteriophage PP01 concentration (concn) was also measured (closed diamonds). (asm.org)
  • 1972-1975: Ahmad Bukhari shows that Mu can insert randomly and prolifically throughout an entire bacterial genome, creating stable insertions. (wikipedia.org)
  • create a crystal structure of the Mu bacteriophage transposome, allowing for a detailed understanding of the process Mu amplification. (wikipedia.org)
  • The dual-component system, which consists of an integrative plasmid with a mini-Mu and an easily eliminated helper plasmid encoding inducible transposition factors, is described in detail as a tool for the integration/amplification of recombinant DNAs. (bireme.br)
  • Many bacteriophages are known to be highly specific for their receptors and show little or no interaction with receptors with an even slightly different structure. (asm.org)
  • S. marcescens bacteriophages were chosen as the focus of our research because of the growing importance of the host and its increasing prevalence as a nosocomial pathogen. (biomedcentral.com)
  • These data reveal structural and functional parallels between bacteriophage Gam and eukaryotic Ku and suggest that their functions have been evolutionarily conserved. (embopress.org)