A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Viruses whose hosts are bacterial cells.
Viruses whose host is Escherichia coli.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.
Proteins found in any species of virus.
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
The functional hereditary units of VIRUSES.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.
Enzymes that recombine DNA segments by a process which involves the formation of a synapse between two DNA helices, the cleavage of single strands from each DNA helix and the ligation of a DNA strand from one DNA helix to the other. The resulting DNA structure is called a Holliday junction which can be resolved by DNA REPLICATION or by HOLLIDAY JUNCTION RESOLVASES.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.
A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.
A broad category of viral proteins that play indirect roles in the biological processes and activities of viruses. Included here are proteins that either regulate the expression of viral genes or are involved in modifying host cell functions. Many of the proteins in this category serve multiple functions.
A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.
The process by which a DNA molecule is duplicated.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Viruses whose nucleic acid is DNA.
An ATP-dependent protease found in prokaryotes, CHLOROPLASTS, and MITOCHONDRIA. It is a soluble multisubunit complex that plays a role in the degradation of many abnormal proteins.
Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7.
Any method used for determining the location of and relative distances between genes on a chromosome.
A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.
A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
The functional hereditary units of BACTERIA.
Proteins found in any species of bacterium.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A highly abundant DNA binding protein whose expression is strongly correlated with the growth phase of bacteria. The protein plays a role in regulating DNA topology and activation of RIBOSOMAL RNA transcription. It was originally identified as a factor required for inversion stimulation by the Hin recombinase of SALMONELLA and Gin site-specific recombinase of BACTERIOPHAGE MU.
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Viruses whose host is Staphylococcus.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A family of bacteriophages which are characterized by short, non-contractile tails.
A class of opioid receptors recognized by its pharmacological profile. Mu opioid receptors bind, in decreasing order of affinity, endorphins, dynorphins, met-enkephalin, and leu-enkephalin. They have also been shown to be molecular receptors for morphine.
Viruses whose host is Streptococcus.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Proteins obtained from ESCHERICHIA COLI.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The sum of the weight of all the atoms in a molecule.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.

An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction. (1/284)

A highly efficient DNA sequencing strategy was developed on the basis of the bacteriophage Mu in vitro DNA transposition reaction. In the reaction, an artificial transposon with a chloramphenicol acetyltransferase (cat) gene as a selectable marker integrated into the target plasmid DNA containing a 10.3-kb mouse genomic insert to be sequenced. Bacterial clones carrying plasmids with the transposon insertions in different positions were produced by transforming transposition reaction products into Escherichia coli cells that were then selected on appropriate selection plates. Plasmids from individual clones were isolated and used as templates for DNA sequencing, each with two primers specific for the transposon sequence but reading the sequence into opposite directions, thus creating a minicontig. By combining the information from overlapping minicontigs, the sequence of the entire 10,288-bp region of mouse genome including six exons of mouse Kcc2 gene was obtained. The results indicated that the described methodology is extremely well suited for DNA sequencing projects in which considerable sequence information is on demand. In addition, massive DNA sequencing projects, including those of full genomes, are expected to benefit substantially from the Mu strategy.  (+info)

SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA). (2/284)

In bacteria, SsrA RNA recognizes ribosomes stalled on defective messages and acts as a tRNA and mRNA to mediate the addition of a short peptide tag to the C-terminus of the partially synthesized nascent polypeptide chain. The SsrA-tagged protein is then degraded by C-terminal-specific proteases. SmpB, a unique RNA-binding protein that is conserved throughout the bacterial kingdom, is shown here to be an essential component of the SsrA quality-control system. Deletion of the smpB gene in Escherichia coli results in the same phenotypes observed in ssrA-defective cells, including a variety of phage development defects and the failure to tag proteins translated from defective mRNAs. Purified SmpB binds specifically and with high affinity to SsrA RNA and is required for stable association of SsrA with ribosomes in vivo. Formation of an SmpB-SsrA complex appears to be critical in mediating SsrA activity after aminoacylation with alanine but prior to the transpeptidation reaction that couples this alanine to the nascent chain. SsrA RNA is present at wild-type levels in the smpB mutant arguing against a model of SsrA action that involves direct competition for transcription factors.  (+info)

Criss-crossed interactions between the enhancer and the att sites of phage Mu during DNA transposition. (3/284)

A bipartite enhancer sequence (composed of the O1 and O2 operator sites) is essential for assembly of the functional tetramer of phage Mu transposase (MuA) on supercoiled DNA substrates. A three-site interaction (LER) between the left (L) and right (R) ends of Mu (att sites) and the enhancer (E) precedes tetramer assembly. We have dissected the role of the enhancer in tetramer assembly by using two transposase proteins that have a common att site specificity, but are distinct in their enhancer specificity. The activity of these proteins on substrates containing hybrid enhancers reveals a 'criss-crossed' pattern of interaction between att and enhancer sites. The left operator, O1, of the enhancer interacts specifically with the transposase subunit at the R1 site (within the right att sequence) that is responsible for cleaving the left end of Mu. The right operator, O2, shows a preferential interaction with the transposase subunit at the L1 site (within the left att sequence) that is responsible for cleaving the right end of Mu.  (+info)

Natural synthesis of a DNA-binding protein from the C-terminal domain of DNA gyrase A in Borrelia burgdorferi. (4/284)

We have identified a 34 kDa DNA-binding protein with an HU-like activity in the Lyme disease spirochete Borrelia burgdorferi. The 34 kDa protein is translated from an abundant transcript initiated within the gene encoding the A subunit of DNA gyrase. Translation of the 34 kDa protein starts at residue 499 of GyrA and proceeds in the same reading frame as full-length GyrA, resulting in an N-terminal-truncated protein. The 34 kDa GyrA C-terminal domain, although not homologous, substitutes for HU in the formation of the Type 1 complex in Mu transposition, and complements an HU-deficient strain of Escherichia coli. This is the first example of constitutive expression of two gene products in the same open reading frame from a single gene in a prokaryotic cellular system.  (+info)

Replacement of the bacteriophage Mu strong gyrase site and effect on Mu DNA replication. (5/284)

The bacteriophage Mu strong gyrase site (SGS) is required for efficient replicative transposition and functions by promoting the synapsis of prophage termini. To look for other sites which could substitute for the SGS in promoting Mu replication, we have replaced the SGS in the middle of the Mu genome with fragments of DNA from various sources. A central fragment from the transposing virus D108 allowed efficient Mu replication and was shown to contain a strong gyrase site. However, neither the strong gyrase site from the plasmid pSC101 nor the major gyrase site from pBR322 could promote efficient Mu replication, even though the pSC101 site is a stronger gyrase site than the Mu SGS as assayed by cleavage in the presence of gyrase and the quinolone enoxacin. To look for SGS-like sites in the Escherichia coli chromosome which might be involved in organizing nucleoid structure, fragments of E. coli chromosomal DNA were substituted for the SGS: first, repeat sequences associated with gyrase binding (bacterial interspersed mosaic elements), and, second, random fragments of the entire chromosome. No fragments were found that could replace the SGS in promoting efficient Mu replication. These results demonstrate that the gyrase sites from the transposing phages possess unusual properties and emphasize the need to determine the basis of these properties.  (+info)

Organization and dynamics of the Mu transpososome: recombination by communication between two active sites. (6/284)

Movement of transposable genetic elements requires the cleavage of each end of the element genome and the subsequent joining of these cleaved ends to a new target DNA site. During Mu transposition, these reactions are catalyzed by a tetramer of four identical transposase subunits bound to the paired Mu DNA ends. To elucidate the organization of active sites within this tetramer, the subunit providing the essential active site DDE residues for each cleavage and joining reaction was determined. We demonstrate that recombination of the two Mu DNA ends is catalyzed by two active sites, where one active site promotes both cleavage and joining of one Mu DNA end. This active site uses all three DDE residues from the subunit bound to the transposase binding site proximal to the cleavage site on the other Mu DNA end (catalysis in trans). In addition, we uncover evidence that the catalytic activity of these two active sites is coupled such that the coordinated joining of both Mu DNA ends is favored during recombination. On the basis of these results, we propose that the DNA joining stage requires a cooperative transition within the transposase-DNA complex. The cooperative utilization of active sites supplied in trans by Mu transposase provides an example of how mobile elements can ensure concomitant recombination of distant DNA sites.  (+info)

Identification of SoxS-regulated genes in Salmonella enterica serovar typhimurium. (7/284)

Salmonella enterica serovar Typhimurium responds to superoxide-generating agents through soxR-mediated activation of the soxS gene, whose product, SoxS, is necessary for resistance to oxidative stress. The S. enterica serovar Typhimurium soxRS system also mediates redox-inducible resistance to diverse antibiotics, which may be relevant to clinical infections. In order to identify SoxS-regulated genes in S. enterica serovar Typhimurium, a lacI-regulated expression system for the S. enterica serovar Typhimurium soxS gene was developed. This system was used to demonstrate that soxS expression is sufficient for the induction of resistance to the superoxide-generating drug paraquat and for the transcriptional activation of the sodA and micF genes. In addition, a library of random lacZ insertions was generated and screened for clones displaying differential beta-galactosidase activity in the presence or absence of SoxS. This selection yielded six independent chromosomal lacZ transcriptional fusions that were activated by either artificial expression of SoxS or exposure of wild-type cells to micromolar concentrations of paraquat. Moreover, disruption of the inducible genes by the insertions rendered S. enterica serovar Typhimurium hypersensitive to millimolar concentrations of paraquat. Nucleotide sequence determination identified the disrupted genes as sodA (Mn-containing superoxide dismutase), fpr (NADPH:ferredoxin oxidoreductase), and ydbK (a putative Fe-S-containing reductase).  (+info)

Mu DNA reintegration upon excision: evidence for a possible involvement of nucleoid folding. (8/284)

Mutations induced by the integration of a Mugem2ts prophage can revert at frequencies around 1x10(-6). In these revertant clones, the prophage excised from its original localization is not lost but reintegrated elsewhere in the host genome. One of the most intriguing aspects of this process is that the prophage reintegration is not randomly distributed: there is a strong correlation between the original site of insertion (the donor site) and the target site of the phage DNA migration (the receptor site). In this paper, it is shown that in the excision-reintegration process mediated by Mugem2ts, the position of the initial prophage site strongly influences the location of the reintegration site. In addition, for each donor site, the receptor site is a discrete DNA region within which the excised Mu DNA can reintegrate and the two sites implicated in phage DNA migration must be located on the same DNA molecule. These data suggest the involvement of nucleoid folding in the excision-reintegration process.  (+info)

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Transposition allows movement of a defined stretch of DNA, a transposon, from one DNA location to another. This process is required for the life-cycles of many viruses, from bacteriophage Mu to HIV; it is spreading antibiotic resistances between bacterial populations; and it is responsible for spontaneous mutations in all the kingdoms of life. Transposition is mediated by a protein, the transposase, encoded by the transposon. DNA sequence signals at the two ends of the transposon activate assembly of a transpososome: a complex that include multiple copies of transposase plus both transposon ends. Transpososome assembly, in turn, activates the DNA cleavage and joining reactions required for transposition. This thesis explores aspects of interactions between one transposase, MuA, and the ends of its transposon DNA, the genome of bacteriophage Mu. The first chapter provides an overview of Mu transposition, with special emphasis on the transpososome. The second chapter shows that in the absence of ...
1BCM: Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.
E. coli ClpX is a member of the Clp/Hsp100 family of ATPases that remodel multi-component complexes and facilitate ATP-dependent protein degradation. Protein remodelers alter the biological activity of their substrates, typically by changing the quaternary structure of their target proteins. ClpX remodels protein-DNA complexes, termed transpososomes, made during recombination of the phage Mu. When recombination is complete, the core four-subunit transpososome complex does not spontaneously release the DNA; transposase remains so stably bound that subsequent replication of the Mu genome is inhibited. To understand how ClpX releases the replication block without destroying the transpososomes, we characterized the mechanism and products of transpososome remodeling. To better understand the mechanism ClpX uses to facilitate remodeling, I first participated in a collaborative project that defined major biochemical reaction steps involved in protein degradation by ClpX and its associated peptidase ...
Background: Tangle analysis has been applied successfully to study proteins which bind two segments of DNA and can knot and link circular DNA. We show how tangle analysis can be extended to model any stable protein-DNA complex. -- Results: We discuss a computational method for finding the topological conformation of DNA bound within a protein complex. We use an elementary invariant from knot theory called colorability to encode and search for possible DNA conformations. We apply this method to analyze the experimental results of Pathania, Jayaram, and Harshey (Cell 2002). We show that the only topological DNA conformation bound by Mu transposase which is biologically likely is the five crossing solution found by Pathania et al (although other possibilities are discussed). -- Conclusion: Our algorithm can be used to analyze the results of the experimental technique described in Pathania et al in order to determine the topological conformation of DNA bound within a stable protein-DNA complex ...
Let $\mathcal{B}_0$ denote the cylinder $\sigma$-algebra. Since a cylinder set $A \in \mathcal{B}_0$ only specifies the values of functions at countably many points, if it is nonempty then it contains a discontinuous function. Hence the inner measure of $C([0,1])$ is $$\mu_*(C([0,1])) = \sup\{\mu(A) : A \in \mathcal{B}_0, A \subset C([0,1])\} = \sup\{\mu(\emptyset)\} = 0.$$ Since $\mu_*(C([0,1])) \ne \mu^*(C([0,1]))$ it is not $\mu$-measurable and is not in the $\mu$-completion of $\mathcal{B}_0$.. ...
Families with certain genetic mutations face many complex and personal choices, including how and when to share such information with their children. Moms Genes is here to help.
Le poper in morda nageljnove žbice imata med začimbami tako bogato in krvavo zgodovino kot muškat. Zdravnikom v Antiki je bil muškat neznan. »Sadovi« muškata so prispeli v Evropo verjetno s križarskimi vitezi. Prvi zanesljivi vir je bizantinski zdravnik Simon Seth, ki je v 10. stoletju napisal o muškatu »da koristi želodcu, jetrom in srcu«, vendar je že tedaj svaril pred pretiranim uživanjem. Najprej so ga po morju in karavanskih poteh prinašali v Evropo arabski trgovci. V tem času je stalo pol kilograma muškata toliko kot tri ovce. V 16. stoletju so ga imenovali »Zlato Vzhodne Indije«. Angleži, Španci, Portugalci in Nizozemci so se krvavo bojevali za sadove muškatnega drevesa. Kot rezultat teh bojev so Angleži zamenjali otok Run v vzhodnoindijskem arhipelagu za veliko večji otok na vhodni obali severne Amerike, ki je bil v nizozemski lasti. Ta otok je Manhattan in je imel tedaj manj kot 1000 prebivalcev. Trgovina z muškatom je prinašala ogromne dobičke. Na otokih je ...
Le poper in morda nageljnove žbice imata med začimbami tako bogato in krvavo zgodovino kot muškat. Zdravnikom v Antiki je bil muškat neznan. »Sadovi« muškata so prispeli v Evropo verjetno s križarskimi vitezi. Prvi zanesljivi vir je bizantinski zdravnik Simon Seth, ki je v 10. stoletju napisal o muškatu »da koristi želodcu, jetrom in srcu«, vendar je že tedaj svaril pred pretiranim uživanjem. Najprej so ga po morju in karavanskih poteh prinašali v Evropo arabski trgovci. V tem času je stalo pol kilograma muškata toliko kot tri ovce. V 16. stoletju so ga imenovali »Zlato Vzhodne Indije«. Angleži, Španci, Portugalci in Nizozemci so se krvavo bojevali za sadove muškatnega drevesa. Kot rezultat teh bojev so Angleži zamenjali otok Run v vzhodnoindijskem arhipelagu za veliko večji otok na vhodni obali severne Amerike, ki je bil v nizozemski lasti. Ta otok je Mahattan in je imel tedaj manj kot 1000 prebivalcev. Trgovina z muškatom je prinašala ogromne dobičke. Na otokih je ...
Using the Tukey method for each of the six comparisons yields: $$ \begin{eqnarray} 0.29 \le & \mu_2 - \mu_1 & \le 4.47 \\ & & \\ 1.13 \le & \mu_3 - \mu_1 & \le 5.31 \\ & & \\ -2.25 \le & \mu_1 - \mu_4 & \le 1.93 \\ & & \\ -2.93 \le & \mu_2 - \mu_3 & \le 1.25 \\ & & \\ 0.13 \le & \mu_2 - \mu_4 & \le 4.31 \\ & & \\ 0.97 \le & \mu_3 - \mu_4 & \le 5.15 \\ \end{eqnarray ...
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CEACAM 5 + 6小鼠单克隆抗体[MUS](ab4539)可与人样本反应并经WB, ELISA, IHC, Flow Cyt实验严格验证,被4篇文献引用。所有产品均提供质保服务,中国75%以上现货。
Ve vojensk m oble en , s kovovou helmou na hlav a atrapami v bu nin se fanou ek Falloutu proch zel centrem m sta, jako by se nechumelilo.
பகல் பன்னிரண்டு மணிக்கு மீண்டும் திருச்சூரை நோக்கிப் புறப்பட்டோம். திருச்சூரைக் கடந்து குன்னங்குளம் என்ற ஊரை நோக்கி எங்கள் மகிழ்வுந்து பறந்தது. நல்ல சாலை அமைப்பு என்பதால் திட்டமிட்டதை விட வேகமாக நாங்கள் திருச்சூரைக் கடந்தோம். வழியில் அண்ணன் வீரக்குமரன் அவர்களின் விரிவுரை மகிழ்வுந்தில் அமைந்தது. முனைவர் வீரக்குமரன் அவர்கள் தமிழ், ஆங்கிலம், மலையாளம் என ...
Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The contributions from the secondary structure of the transcriptional activator protein C of bacteriophage Mu to its specific DNA binding and the influence of various factors, viz ., electrolytes, and minor groove and major groove binders on this protein-DNA interaction have been addressed . Circular dichroism (CD) spectral results suggest that, in the absence of Mg2+, C protein exhibits a j3-pleated sheetlike structure and Mg2+ changes the conformation to a more a-helical structure which could provide specific geometrical constraints complementary to those of DNA helix. Thus, Mg 2+ acts as a cofactor for the binding of the C protein to its specific site in DNA by inducing conformational changes in the protein. Competitive binding studies with minor and major groove binding drugs, viz ., distamycin A and methyl green, respectively, and the DMS footprinting data indicate that the C protein recognizes the major groove of DNA during complex formation . Further, upon major groove binding, C protein ...
Hsmar1, a cut-and-paste element of the mariner transposon family, was recently investigated to examine the kinetics of the complex formation pathway. This transposase forms a dimer in solution before binding to first one transposon end, then recruiting the other. This pathway, Synapsis by Naked End Capture (S-NEC), is found in both prokaryotes and eukaryotes. Tn5, of the IS4 family, is an example of the alternative pathway, Synapsis by Protein Dimerisation (S-PD). In this pathway, the transposase is monomeric in solution. Each monomer binds to a transposon end independently, before combining to form a synaptic complex. This pathway has been seen widely in prokaryotes, but no eukaryotic elements tested to date employ it ...
Like AZT, 1NP also inhibited 4MU glucuronidation by UGT2B7 without a substantial effect on substrate cooperativity (at least at low 1NP concentrations). However, inhibition resulted mainly from a decrease in Vmax. The model used to describe these data again assumes binding of inhibitor at a distinct effector site but with abolition of product formation from enzyme complexes containing inhibitor. As is evident from Fig. 7, A and C, rate of product formation approaches zero at high modifier concentration. Although experimental data were well described by the three-site model, the increase in S50 and decrease in Hill coefficient observed at the highest modifier concentrations suggests that 1NP may also act as a competitive inhibitor of 4MU glucuronidation.. In contrast to the inhibition of 4MU glucuronidation by 1NP, 4MU activated the formation of 1NPG by UGT2B7. Kinetic data were well described by the three-site model illustrated in Fig. 2B, where modifier binds at an effector site that is ...
Having professional family photos taken can be an ordeal, but its basically a necessary one to endure. Because at the end of the day, who doesnt love having a perfect-looking family portrait to hang on the wall?. Unless youre a member of the Zaring family, that is. Because their recent family photos are going viral for being hilariously awful.. Pam Zaring is a mom who just wanted some nice family pictures like any mom does. So she sought out the services of a professional photographer in her area, paid $250 for said services, and gathered her entire family and two dogs together for some scenic photos on a warm, sunny day.. The results? OMG. You just have to see them for yourself. You. Will. Die.. LOL forever. When family pictures go wrong, usually its because a kid is crying or someones eyes are closed. In this case, its the, uh, interesting editing choices.. Please see these FOR REAL photos she delivered to us, Zaring wrote in the post. She said the shadows were really bad on the ...
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
Tzv. INSPIRE prioritní data jsou data, která spadají do některého z témat INSPIRE (příloha III) a zároveň jsou to data, kterými je naplňován reporting v oblasti životního prostředí. Jedná se o tematické oblasti: Voda, Kvalita ovzduší & Hluk, Příroda & Biodiverzita, Opady a Průmysl (Mořské oblasti nejsou pro ČR relevantní). Pro tato prioritní data musí být zpřístupněna metadata tak, aby je bylo možné přímo zobrazit a stáhnout ...
Question - what is the best way for the treatment for mu tumer and who - 5Y. Find the answer to this and other Neurology questions on JustAnswer
TY - JOUR. T1 - (+)-CC-1065 as a structural probe of Mu transposase-induced bending of DNA. T2 - Overcoming limitations of hydroxyl-radical footprinting. AU - Ding, Zhi Ming. AU - Harshey, Rasika M.. AU - Hurley, Laurence. PY - 1993/9/11. Y1 - 1993/9/11. N2 - Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90°. To probe the directionality and locus of these A-protein-induced bends, we have used the antitumor antibiotic (+)-CC-1065 as a structural probe. As a consequence of binding within the minor groove, (+)-CC-1065 is able to alkylate N3 of adenine in a sequence selective manner. This selectivity is partially determined by conformational flexibility of the DNA sequence, and the covalent adduct has a bent DNA structure in ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Additional engineered enzymes (not included in the present design) may be needed to digest bacteriophages that may be resident inside certain bacteria. To avoid digestion by bacterial restriction enzymes, phages often employ unusual molecular substitutions involving 2,6-diaminopurine, 6-methyladenine, 8-azaguanine, 5-hydroxymethyl uracil, 5-methylcytosine, 5-hydroxymethylcytosine, and others [121]. For example, B. subtilis phage DNA replaces thymine with hydroxymethyluracil and uracil; S-2L cyanophage replaces adenine by 2-aminoadenine (2,6-diaminopurine); SPO1, SP82G, and Phi-e substitute hydroxymethyl dUTP for dTTP in the phage DNA up to 20%; PBS1 and PBS2 phages substitute uracil for thymine; T-even (T2/T4/T6) phage DNA replaces dCMP by hydroxymethylcytosine which is then further glycosylated, rendering the phage DNA resistant to host restriction; and in phage Mu DNA, a unique glycinamide moiety modifies about 15% of the adenine residues [121]. Given our complete future knowledge of phage ...
Abrahams, P. J., Mulder, C., Vandevoorde, A., Warnaar, S. O., Vandereb, A. J. (1975) Transformation of Primary Rat-Kidney Cells by Fragments of Simian Virus 40 DNA. Journal of Virology, 16 (4). pp. 818-823. Allet, Bernard, Bukhari, Ahmad I. (March 1975) Analysis of Bacteriophage Mu and Lambda-Mu Hybrid Dnas by Specific Endonucleases. Journal of Molecular Biology, 92 (4). pp. 529-536. Apte, B. N., Rhodes, H., Zipser, D. (September 1975) Mutation blocking the specific degradation of reinitiation polypeptides in E. coli. Nature, 257 (5524). pp. 329-31. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of Terminal Repetition in Adenovirus 2 DNA. Cold Spring Harbor Symposia on Quantitative Biology, 39. pp. 401-407. Arrand, J. R., Keller, W., Roberts, R. J. (1975) Extent of terminal repetition in adenovirus 2 DNA. Cold Spring Harb Symp Quant Biol, 39 Pt . pp. 401-7. Atkins, J. F., Gesteland, R. F. (August 1975) The synthetase gene of the RNA phages R17, MS2 and f2 has a single UAG terminator ...
Indicatii: Eliminarea parazitilor intestinali in cure de 7 zile repetate dupa o pauza de alte 7 zile pentru prevenirea recidivelor. Femelele măsoară până la zece milimetri în lungime și ajung să efectueze ouatul spre rect, unde cu mușcăturile lor provoacă prurit enervant pe marginea anusului. Apasă pentru a vedea definiția originală «oxiuro» în dicționarul Spaniolă dictionary.
Konsolidovaný počiatočný finančný výkaz Eurosystému k 1. januáru 2011a konsolidovaný finančný výkaz Eurosystému k 7. januáru 2011
Argentina - Servidor de Mu Online dedicado Multilenguaje Internacional en Español .:Mu Online:. MuOnline Server 1.04D - Season 6 EP3 - Ranking Elfas Top Reset Master - Argentina
Prečo je pre mužov/ženy análny styk zaujímavý (Tesnejšie zovretie? Veľa ľudí chce svoj sex v posteli okoreniť, siahajú preto po erotických pomôckach. Análny sex. Pokiaľ nie ste vopred dohodnutí, nepchaj sa tam, kde nemáš.
Viah to bad gin gin lau badle emoji videos, Viah Ton Baad - Sukhy Maan | Dash Records | Latest Punjabi Songs 2016, gin lau, gin lau, Viah to Baad gin gin Lau badle. Emoji video, gin lau and jacky li, 2NE1 - 내가 제일 잘 나가(I AM THE BEST) M/V
MU Online Season 6 Private Server Medium x1000 - x100 Dynamic. Added new custom boxes, boses, events, items, 4 level wings and a lot of other attractions
Diabetik je človek trpiaci cukrovkou a jeho život sa chorobe musí prispôsobiť. Pokiaľ sa diabetik riadi radami lekárov a dodržuje liečbu, môže prežuť plnohodnotný život.
Vo víkendovej Veľkej cene Singapuru formuly 1 najviac zaujala havária z úvodného kola, po ktorej skončilo viacero pretekárov.
Busy Moms is a community for Moms on the move, here you will find helpful information for working and stay-at-home Moms. We hope you enjoy ...
Kuba wahinga byumwuga ngo ntabwo bisaba kuba warabyize cyangwa ufite amafaranga menshi cyane nkuko bisobanurwa numuhinzi twasuye kugira ngo adusobanurire ibyo guhinga no korora kijyambere mu Rwanda. Emmanuel Ntuyenabo wize ubwubatsi ubu ni umuhinzi mworozi wabigize umwuga kandi amaze kugera kuri byinshi mu myaka itandatu abimazemo. Yatangije miliyoni umunani, ubu umutungo we wikubye inshuro nyinshi …. Uko wakura ubukire mu mwuga wubuhinzi nubworozi Read More ». ...
To say gin is having a moment would underestimate its popularity, and they can be as varied as they are delicious: fruity, floral, spicy or herbal. Whatever your preference, theres a gin for you - but tonic is another story. Find out more about how tonic is made and how to match it to your gin.
To say gin is having a moment would underestimate its popularity, and they can be as varied as they are delicious: fruity, floral, spicy or herbal. Whatever your preference, theres a gin for you - but tonic is another story. Find out more about how tonic is made and how to match it to your gin.
Mikroalbuminurie zvyšuje u diabetiků výrazně nejen riziko vývoje diabetické nefropatie, ale i kardiovaskulárních komplikací. Zbývající část článku naleznete v přiloženém PDF.
Există în corp şi unele zone fără bacterii în mod normal: muşchii, sângele şi sistemul nervos. Bacterii autotrofe fotosintetizante care folosesc energia solară pentru producerea de substanţe organice.
+Analysis andIsolation ofPlasminogenActivator fromMammalian CellCulture BrothDelaine Zayas-Bazán BurgosJohn Muñoz TorresVibha Bansal PhDMr. Javier RosadoMr. Ca…
Researchers define turning points as a major transformation in views about the self, identity or the meaning of life. They occur as new things are learned, rendering us amenable to change, and produce perceived, long-lasting redirection in the path of a ones life. Psychologists associate turning points with transitions and stages of human development defined and explored by Erik Erikson. Ignoring uplifting turning points and with distressing turning points in mind, the philosopher Frederick Nietzsche wrote that which does not kill us makes us stronger. Retirement or loss of retirement income, end of a love affair, reaching the golden years (maturity) or learning that one (or a family member) has a fatal disease are examples of turning points. Portrayals, in film and literature, of individuals coping with obstacles to happiness or overcoming adversity dramatize turning points. Rhetorical, films and literature are cultural artifacts that comfort, guide generations and teach us how to live! Lessons
TY - JOUR. T1 - Genomic DNA transposition induced by human PGBD5. AU - Henssen, Anton G.. AU - Henaff, Elizabeth. AU - Jiang, Eileen. AU - Eisenberg, Amy R.. AU - Carson, Julianne R.. AU - Villasante, Camila M.. AU - Ray, Mondira. AU - Still, Eric. AU - Burns, Melissa. AU - Gandara, Jorge. AU - Feschotte, Cedric. AU - Mason, Christopher E.. AU - Kentsis, Alex. N1 - Publisher Copyright: © Henssen et al.. PY - 2015/9/25. Y1 - 2015/9/25. N2 - Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its ...
Transposase is an enzyme that binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism. The word transposase was first coined by the individuals who cloned the enzyme required for transposition of the Tn3 transposon. The existence of transposons was postulated in the late 1940s by Barbara McClintock, who was studying the inheritance of maize, but the actual molecular basis for transposition was described by later groups. McClintock discovered that pieces of the chromosomes changed their position, jumping from one chromosome to another. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed. Transposition in maize causes changes in color; however, in other organisms, such as bacteria, it can cause antibiotic resistance. Transposition is also important in creating genetic diversity within species and adaptability to ...
SCOTTSDALE, Ariz. - August marks a significant 10-year anniversary for patients with digestive problems. Just one decade ago, in August 2001, the FDA approved use of capsule endoscopy technology to examine the last frontier of the human GI tract - the small bowel.. Mayo Clinic in Arizona was among the first medical centers in the U.S. to perform exams of the small bowel using the pioneering pill camera technology that can scan the far corners of the GI tract - painlessly and without surgery.. Over the past decade, Mayo Clinic as a three-site organization (Arizona, Florida and Minnesota) has completed more than 7,000 such exams, where patients swallow a vitamin-sized capsule that contains a miniature camera. The capsule then travels through the digestive tract, snapping thousands of pictures that are transmitted to a recorder that patients wear around their waist for eight to 12 hours. Images saved on the recorder are transferred to a computer to create a video of the digestive tract, ...
Zimwe mu nzara zizwi zabayeho, hari inzara ya Rukungugu yabaye ku ngoma yUmwami Yuhi IV Gahindiro. Iyo nzara yabaye ahagana mu ntangiriro zikinyejana cya XIX. Yatewe namapfa yiganje mu gihugu. Nkuko Padiri Alexis Kagame abyandika mu gitabo cye yise « Un Abrégé de lhistoire du Rwanda », Umwami Gahindiro nabiru bakoresheje icyo gihe Inzira yubwiru ya Rukungugu mu kuyirwanya. Iyo nzara yamaze umwaka umwe.. Nyuma yaho, mu gihe cyubukoloni bwAbadage, mu Rwanda habaye nizindi nzara, zirimo Ruyaga yo mu 1897-1903, Rwakabaga cyangwa se Kimwaramwara yo mu 1906-1909. Inzara Ruyaga yatewe ninzige zateye imyaka, zikayona kugeza aho bivuyemo inzara. Naho Inzara ya Rwakabaga yatewe namapfa yiganje cyane mu majyaruguru no mu burasirazuba bwigihugu.. Mu gihe cyubukoloni bwAbabiligi, u Rwanda rwatewe nizindi nzara zikomeye. Izo ni nka Rumanura cyangwa se Rumanurimbaba yo mu ntambara ya mbere yisi yose. Iyo nzara yabaye hagati yi 1916 ni 1918. Yatewe nintambara, ihunga ryabantu, ubusahuzi ...
displaystyle {\begin{aligned}{\mathcal {L}}_{\mathrm {QCD} }&={\bar {\psi }}_{i}\left(i\gamma ^{\mu }(D_{\mu })_{ij}-m\,\delta _{ij}\right)\psi _{j}-{\frac {1}{4}}G_{\mu \nu }^{a}G_{a}^{\mu \nu }\\&={\bar {\psi }}_{i}(i\gamma ^{\mu }\partial _{\mu }-m)\psi _{i}-gG_{\mu }^{a}{\bar {\psi }}_{i}\gamma ^{\mu }T_{ij}^{a}\psi _{j}-{\frac {1}{4}}G_{\mu \nu }^{a}G_{a}^{\mu \nu }\,,\\\end{aligned ...
0/0. 25 kr. (od 5 krok 5 kred). Přípravu DISERTAČNÍ PRÁCE hodnotí a určuje školitel po dohodě se studentem. Počet kreditů je minimálně 5, max. 25. Součet kreditů předmětu Příprava dis.práce za celé studium musí být minim. 120, max. 160. Ukončení: z ...
This paper derives (by a new method) an equation due to Macdonald for determining the zeros of the associated Legendre functions of order m and non-integral degree n when the argument is close to -1. A closed form solution is obtained for the values of Q subscript n superscript m (mu) and Q subscript n superscript -m (mu) for mu close to 1. Certain observations are made concerning errors in a recently published article.(*RADAR CROSS SECTIONS
TOP ponuka nehnuteľností online z Mučína aktuálny prehľad realít vo vašom okolí. Reality Mučín od realitiek aj od súkromných osôb online na Reality.sk
OMA MUA (FI) i Tidningsarkivet. Ett digitalt arkiv för svenska tidningar och tidskrifter. Här finns bland annat omslag och innehållstexter för OMA MUA (FI).
Dozens of cases of the Mu COVID-19 variant - recently declared a variant of interest - have been identified in B.C. According to the B.C. Centre for Disease Controls latest weekly update on . . .
Bukeye umukobwa numuhungu, mu masaha yigicamunsi barahura ngo batembere, sinzi uko umuhungu yaje kurabukwa igishyimbo mu menyo yumukobwa, umuhungu aba uwo munsi yari afite twa duti dukura ibiryo mu menyo, amuha kamwe agira ati Akira ukure ifiriti mu menyo. ...
During the Fourth Eurosymposium on Healthy Ageing (EHA), we had the opportunity to meet Dr. Daniel Muñoz-Espín from the University of Cambridge.
12 Benefits of Breastfeeding for Both Mom and Baby - the benefits of breastfeeding for both mom and baby include - breastfeeding for both mom and baby
How many moms were...... - Just thought it would be intersting to see how many moms were high risk, why and how many week you were when you... -...
Mužský a ženský organizmus má svoje špecifiká. Súvisí to aj s evolúciou, v rámci ktorej sa u mužov vyvinula reakcia „útok alebo útek, ktorá znamenala „prežiť a zároveň slúžila ako výberový mechanizmus, kedy prežili iba tí najsilnejší jedinci. Všetko, čo potrebuje mužský organizmus, obsahuje FOR MAN:. ...
Today we we remind you of a few reasons why your mom is the best...just in time for Mothers Day! Here are 25 Reasons You Should Thank Your Mom!
Worried mom - Im new to this site, have many questions so please be patient. My daughter is 19 and is dating a 17 year old is this normal,...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40 ...
Üniversitemiz bünyesinde dokuz fakülte, iki enstitü ve altı meslek yüksekokulu bulunmaktadır. MŞÜ, topluma ve yaklaşık 9000 öğrencisine, 580 akademik ve 391 idari personeli ile 166 derslik, 24 laboratuvar ve toplantı salonunda; merkezi laboratuvarları ve müstakil etkinlik alanlarında hizmet vermektedir.
Španiel Alberto Contador z tímu Tinkoff-Saxo sa vrátil v sobotňajšej dlhej časovke na 59,4 km z Trevisa do Valdobbiadene do ružového dresu vedúceho muža cyklistických pretekov Giro dItalia.. ...

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Bacteriophage Growth Media. *Circlegrow® Bacterial Growth Medium. *Yeast*S. cerevisiae Growth Media*Standard Growth Media ...
Bacteriophage Growth Media. *Circlegrow® Bacterial Growth Medium. *Yeast*S. cerevisiae Growth Media*Standard Growth Media ...
Bacteriophage Growth Media. *Circlegrow® Bacterial Growth Medium. *Yeast*S. cerevisiae Growth Media*Standard Growth Media ...
Bacteriophage Growth Media. *Circlegrow® Bacterial Growth Medium. *Yeast*S. cerevisiae Growth Media*Standard Growth Media ...
Bacteriophage Growth Media. *Circlegrow® Bacterial Growth Medium. *Yeast*S. cerevisiae Growth Media*Standard Growth Media ...

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