Bacteriophage M13: Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.Coliphages: Viruses whose host is Escherichia coli.Bacteriophages: Viruses whose hosts are bacterial cells.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.Alkynes: Hydrocarbons with at least one triple bond in the linear portion, of the general formula Cn-H2n-2.Gold: A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Azides: Organic or inorganic compounds that contain the -N3 group.Metal Nanoparticles: Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.Laboratory ManualsManuals as Topic: Books designed to give factual information or instructions.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Histology: The study of the structure of various TISSUES of organisms on a microscopic level.Andrology: A scientific or medical discipline concerning the study of male reproductive biology, diseases of the male genital organs, and male infertility. Major areas of interest include ENDOCRINOLOGY; SPERMATOGENESIS; semen analysis; FERTILIZATION; CONTRACEPTION; and CRYOPRESERVATION.Semen Analysis: The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Sterilization: The destroying of all forms of life, especially microorganisms, by heat, chemical, or other means.Erythema Nodosum: An erythematous eruption commonly associated with drug reactions or infection and characterized by inflammatory nodules that are usually tender, multiple, and bilateral. These nodules are located predominantly on the shins with less common occurrence on the thighs and forearms. They undergo characteristic color changes ending in temporary bruise-like areas. This condition usually subsides in 3-6 weeks without scarring or atrophy.Sterilization, Tubal: Procedures that render the female sterile by interrupting the flow in the FALLOPIAN TUBE. These procedures generally are surgical, and may also use chemicals or physical means.Sterilization, Reproductive: Procedures to block or remove all or part of the genital tract for the purpose of rendering individuals sterile, incapable of reproduction. Surgical sterilization procedures are the most commonly used. There are also sterilization procedures involving chemical or physical means.Leprosy, Lepromatous: A chronic communicable infection which is a principal or polar form of LEPROSY. This disorder is caused by MYCOBACTERIUM LEPRAE and produces diffuse granulomatous skin lesions in the form of nodules, macules, or papules. The peripheral nerves are involved symmetrically and neural sequelae occur in the advanced stage.Sterilization Reversal: Procedures to reverse the effect of REPRODUCTIVE STERILIZATION and to regain fertility. Reversal procedures include those used to restore the flow in the FALLOPIAN TUBE or the VAS DEFERENS.Ossification, Heterotopic: The development of bony substance in normally soft structures.Protein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Neurogranin: A BRAIN-specific substrate for PROTEIN KINASE C that binds CALMODULIN and is involved in regulation of CALCIUM SIGNALING.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Deoxyribonucleases, Type II Site-Specific: Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.Peptide Library: A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.Protein Engineering: Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Jupiter: The fifth planet in order from the sun. It is one of the five outer planets of the solar system. Its sixteen natural satellites include Callisto, Europa, Ganymede, and Io.Antibody Affinity: A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.DNA (Cytosine-5-)-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA.Site-Specific DNA-Methyltransferase (Adenine-Specific): An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.Gene Fusion: The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.Methyltransferases: A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.Chromosomes, Artificial, Human: DNA constructs that are composed of, at least, all elements, such as a REPLICATION ORIGIN; TELOMERE; and CENTROMERE, required for successful replication, propagation to and maintainance in progeny human cells. In addition, they are constructed to carry other sequences for analysis or gene transfer.Centromere: The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.Telomere: A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.Hospitals, General: Large hospitals with a resident medical staff which provides continuous care to maternity, surgical and medical patients.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Professional Corporations: Legally authorized corporations owned and managed by one or more professionals (medical, dental, legal) in which the income is ascribed primarily to the professional activities of the owners or stockholders.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.

Identification of residues in beta-lactamase critical for binding beta-lactamase inhibitory protein. (1/356)

beta-Lactamase inhibitory protein (BLIP) is a potent inhibitor of several beta-lactamases including TEM-1 beta-lactamase (Ki = 0.1 nM). The co-crystal structure of TEM-1 beta-lactamase and BLIP has been solved, revealing the contact residues involved in the interface between the enzyme and inhibitor. To determine which residues in TEM-1 beta-lactamase are critical for binding BLIP, the method of monovalent phage display was employed. Random mutants of TEM-1 beta-lactamase in the 99-114 loop-helix and 235-240 B3 beta-strand regions were displayed as fusion proteins on the surface of the M13 bacteriophage. Functional mutants were selected based on the ability to bind BLIP. After three rounds of enrichment, the sequences of a collection of functional beta-lactamase mutants revealed a consensus sequence for the binding of BLIP. Seven loop-helix residues including Asp-101, Leu-102, Val-103, Ser-106, Pro-107, Thr-109, and His-112 and three B3 beta-strand residues including Ser-235, Gly-236, and Gly-238 were found to be critical for tight binding of BLIP. In addition, the selected beta-lactamase mutants A113L/T114R and E240K were found to increase binding of BLIP by over 6- and 11-fold, respectively. Combining these substitutions resulted in 550-fold tighter binding between the enzyme and BLIP with a Ki of 0.40 pM. These results reveal that the binding between TEM-1 beta-lactamase and BLIP can be improved and that there are a large number of sequences consistent with tight binding between BLIP and beta-lactamase.  (+info)

Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. (2/356)

We have genetically modified filamentous bacteriophage to deliver genes to mammalian cells. In previous studies we showed that noncovalently attached fibroblast growth factor (FGF2) can target bacteriophage to COS-1 cells, resulting in receptor-mediated transduction with a reporter gene. Thus, bacteriophage, which normally lack tropism for mammalian cells, can be adapted for mammalian cell gene transfer. To determine the potential of using phage-mediated gene transfer as a novel display phage screening strategy, we transfected COS-1 cells with phage that were engineered to display FGF2 on their surface coat as a fusion to the minor coat protein, pIII. Immunoblot and ELISA analysis confirmed the presence of FGF2 on the phage coat. Significant transduction was obtained in COS-1 cells with the targeted FGF2-phage compared with the nontargeted parent phage. Specificity was demonstrated by successful inhibition of transduction in the presence of excess free FGF2. Having demonstrated mammalian cell transduction by phage displaying a known gene targeting ligand, it is now feasible to apply phage-mediated transduction as a screen for discovering novel ligands.  (+info)

Selection-dominant and nonaccessible epitopes on cell-surface receptors revealed by cell-panning with a large phage antibody library. (3/356)

To generate antibodies to defined cell-surface antigens, we used a large phage antibody fragment library to select on cell transfectants expressing one of three chosen receptors. First, in vitro panning procedures and phage antibody screening ELISAs were developed using whole live cells stably expressing the antigen of interest. When these methodologies were applied to Chinese hamster ovary (CHO) cells expressing one of the receptors for a neuropeptide, somatostatin, using either direct cell panning or a strategy of depletion or ligand-directed elution, many different pan-CHO-cell binders were selected, but none was receptor specific. However, when using direct panning on CHO-cells expressing the human membrane protein CD36, an extraordinary high frequency of antigen-specific phage antibodies was found. Panning on myoblasts expressing the rat homologue of CD36 revealed a similar selection dominance for anti-(CD36). Binding of all selected 20 different anti-(CD36) phage was surprisingly inhibited by one anti-(CD36) mAb CLB-IVC7, which recognizes a functional epitope that is also immunodominant in vivo. Similar inhibition was found for seven anti-(rat) CD36 that cross-reacted with human CD36. Our results show that, although cells can be used as antigen carriers to select and screen phage antibodies, the nature of the antigen target has a profound effect on the outcome of the selection.  (+info)

Identification of peroxisomal proteins by using M13 phage protein VI phage display: molecular evidence that mammalian peroxisomes contain a 2,4-dienoyl-CoA reductase. (4/356)

To elucidate unknown mammalian peroxisomal enzymes and functions, we subjected M13 phage expressing fusions between the gene encoding protein VI and a rat liver cDNA library to an immunoaffinity selection process in vitro (biopanning) with the use of antibodies raised against peroxisomal subfractions. In an initial series of biopanning experiments, four different cDNA clones were obtained. These cDNA species encoded two previously identified peroxisomal enzymes, catalase and urate oxidase, and two novel proteins that contained a C-terminal peroxisomal targeting signal (PTS1). A primary structure analysis of these novel proteins revealed that one, ending in the tripeptide AKL, is homologous to the yeast peroxisomal 2,4-dienoyl-CoA reductase (EC 1.3.1.34; DCR), an enzyme required for the degradation of unsaturated fatty acids, and that the other, ending in the tripeptide SRL, is a putative member of the short-chain dehydrogenase/reductase (SDR) family, with three isoforms. Green fluorescent protein (GFP) fusions encoding GFP-DCR-AKL, GFP-DCR, GFP-SDR-SRL and GFP-SDR were expressed in mammalian cells. The analysis of the subcellular location of the recombinant fusion proteins confirmed the peroxisomal localization of GFP-DCR-AKL and GFP-SDR-SRL, as well as the functionality of the PTS1. That the AKL protein is indeed an NADPH-dependent DCR was demonstrated by showing DCR activity of the bacterially expressed protein. These results demonstrate at the molecular level that mammalian peroxisomes do indeed contain a DCR. In addition, the results presented here indicate that the protein VI display system is suitable for the isolation of rare cDNA clones from cDNA libraries and that this technology facilitates the identification of novel peroxisomal proteins.  (+info)

Peptide ligands to human immunodeficiency virus type 1 gp120 identified from phage display libraries. (5/356)

We have used phage-displayed peptide libraries to identify novel ligands to the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120. Screening of libraries of random 12-mers, 7-mers, and cyclic 9-mers produced two families of gp120 binding peptides. Members of a family with the prototype sequence RINNIPWSEAMM (peptide 12p1) inhibit the interaction between gp120 and both four-domain soluble CD4 (4dCD4) and monoclonal antibody (MAb) 17b, a neutralizing antibody that covers the chemokine receptor binding surface on gp120. Peptide 12p1 inhibits the interaction of 4dCD4 with gp120 from three different HIV strains, implying that it binds to a conserved site on gp120. Members of a second family of peptides, with the prototype sequence TSPYEDWQTYLM (peptide 12p2), bind more weakly to gp120. They do not detectably affect its interaction with 4dCD4, but they enhance its binding to MAb 17b. A common sequence motif in the two peptide families and cross-competition for gp120 binding suggest that they have overlapping contacts. Their divergent effects on the affinity of gp120 for MAb 17b may indicate that their binding stabilizes distinct conformational states of gp120. The functional properties of 12p1 suggest that it might be a useful lead for the development of inhibitors of HIV entry.  (+info)

Peptide ligands that bind IgM antibodies and block interaction with antigen. (6/356)

We have selected a peptide-display phage library on IgM Abs and identified a panel of phage-expressing peptides that bind to IgM Abs in general, but not to Abs of other classes. A synthetic peptide corresponding to one of the displayed peptide sequences also binds to IgM Abs. The peptides bind to both soluble pentameric Abs and to monomeric cell-surface IgM. The phage-displayed and synthetic peptides inhibit the binding of IgM Abs to Ag. These peptides may create confounding artifacts when IgM Abs are used for epitope mapping studies. Nonetheless, the peptides may have both experimental and therapeutic utility.  (+info)

Selection of a C5a receptor antagonist from phage libraries attenuating the inflammatory response in immune complex disease and ischemia/reperfusion injury. (7/356)

A C5a-receptor antagonist was selected from human C5a phage display libraries in which the C terminus of des-Arg74-hC5a was mutated. The selected molecule is a competitive C5a receptor antagonist in vitro and in vivo. Signal transduction is interrupted at the level of G-protein activation. In addition, the antagonist does not cause any C5a receptor phosphorylation. Proinflammatory properties such as chemotaxis or lysosomal enzyme release of differentiated U937 cells, as well as C5a-induced changes in intracellular Ca2+ concentration of murine peritoneal macrophages, are inhibited. The in vivo efficacy was evaluated in three different animal models of immune complex diseases in mice, i.e., the reverse passive Arthus reaction in the peritoneum, skin, and lung. The i.v. application of the C5a receptor antagonist abrogated polymorphonuclear neutrophil accumulation in peritoneum and markedly attenuated polymorphonuclear neutrophil migration into the skin and the lung. In a model of intestinal ischemia/reperfusion injury, i.v. administration of the C5a receptor antagonist decreased local and remote tissue injury: bowel wall edema and hemorrhage as well as pulmonary microvascular dysfunction. These data give evidence that C5a is an important mediator triggering the inflammatory sequelae seen in immune complex diseases and ischemia/reperfusion injury. The selected C5a receptor antagonist may prove useful to attenuate the inflammatory response in these disorders.  (+info)

New inhibitors of Helicobacter pylori urease holoenzyme selected from phage-displayed peptide libraries. (8/356)

Urease is an important virulence factor for Helicobacter pylori and is critical for bacterial colonization of the human gastric mucosa. Specific inhibition of urease activity has been proposed as a possible strategy to fight this bacteria which infects billions of individual throughout the world and can lead to severe pathological conditions in a limited number of cases. We have selected peptides which specifically bind and inhibit H. pylori urease from libraries of random peptides displayed on filamentous phage in the context of pIII coat protein. Screening of a highly diverse 25-mer combinatorial library and two newly constructed random 6-mer peptide libraries on solid phase H. pylori urease holoenzyme allowed the identification of two peptides, 24-mer TFLPQPRCSALLRYLSEDGVIVPS and 6-mer YDFYWW that can bind and inhibit the activity of urease purified from H. pylori. These two peptides were chemically synthesized and their inhibition constants (Ki) were found to be 47 microM for the 24-mer and 30 microM for the 6-mer peptide. Both peptides specifically inhibited the activity of H. pylori urease but not that of Bacillus pasteurii.  (+info)

*Biopanning

The most often used are genes pIII or pVIII of bacteriophage M13. The next step is the capturing step. It involves conjugating ... This involves inserting foreign desired gene segments into a region of the bacteriophage genome, so that the peptide product ... It utilizes the binding interactions so that only specific peptides presented by bacteriophage are bound to the target. For ... The end result is the peptides produced by bacteriophage are specific. The resulting filamentous phages can infect gram- ...

*Helicos single molecule fluorescent sequencing

This sequencing method and equipment were used to sequence the genome of the M13 bacteriophage. The Helicos Genetic Analysis ...

*Geminiviridae

Geminiviruses replicate via a rolling circle mechanism like bacteriophages such as M13, and many plasmids. Replication occurs ...

*Cloning vector

The bacteriophages used for cloning are the phage λ and M13 phage. There is an upper limit on the amount of DNA that can be ... Some plasmids contain an M13 bacteriophage origin of replication and may be used to generate single-stranded DNA. These are ... Cosmids are plasmids that incorporate a segment of bacteriophage λ DNA that has the cohesive end site (cos) which contains ... Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages ( ...

*Phage major coat protein

"Molecular models and structural comparisons of native and mutant class I filamentous bacteriophages Ff (fd, f1, M13), If1 and ... These bacteriophages are flexible rods, about one to two micrometres long and six nm in diameter, with a helical shell of ... a phage major coat protein is an alpha-helical protein that forms a viral envelope of filamentous bacteriophages. ...

*Bacterial display

... when peptides were genetically fused with proteins displayed on the M13 bacteriophage. Bacteriophage display is a commonly used ... Bacteriophage display is the most common type of display system used although bacterial display is becoming increasingly ... using bacterial display systems is that the whole bacterial cell can be incorporated in the live vaccine Unlike bacteriophage ...

*Genetically modified virus

... the E4 bacteriophage and the M13 bacteriophage, to be used as a cathode. This was done by editing the genes of the virus that ...

*Angela Belcher

... known as the M13 bacteriophage whose target is usually Escherichia coli. M13 can be made to latch onto and coat itself with ...

*Phage display

The most common bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also ... Many genetic sequences are expressed in a bacteriophage library in the form of fusions with the bacteriophage coat protein, so ... In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or ... Malys N, Chang DY, Baumann RG, Xie D, Black LW (2002). "A bipartite bacteriophage T4 SOC and HOC randomized peptide display ...

*David R. Liu

... a technique that uses the short 10-minute lifespan of the M13 bacteriophage to achieve the rapid evolution of useful proteins. ...

*Bacteriophage

M13 Leviviridae. Nonenveloped, isometric. Linear ssRNA. MS2, Qβ Microviridae. Nonenveloped, isometric. Circular ssDNA. ΦX174 ... Bacteriophages are among the most common and diverse entities in the biosphere.[1] Bacteriophages are ubiquitous viruses, found ... A bacteriophage (/bækˈtɪərioʊfeɪdʒ/), also known informally as a phage (/feɪdʒ/), is a virus that infects and replicates within ... 2×108 bacteriophages per mL.[47] Bacteriophages are thought to extensively contribute to horizontal gene transfer in natural ...

*Phage (disambiguation)

... temperate bacteriophage that infects Escherichia coli M13 phage, filamentous bacteriophage composed of circular single stranded ... Phage is the shortened form of bacteriophage, a virus that infects bacteria. Phage (from Greek φαγεῖν phagein, 'to eat') may ... the best-studied bacteriophage of the family Cystoviridae T7 phage, phage capable of infecting susceptible bacterial cells In ... the study of the interaction of bacteriophage with their environments Phage monographs, books published on the topic of ...

*M13 bacteriophage

M13 does not have this unique Bam site in gene III. M13 had to be engineered to have accessible insertion sites, making it ... M13 bacteriophage structure in the protein data bank [2]. ... M13 is a virus that infects the bacterium Escherichia coli. It ... M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures ... Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more ...

*Filamentous bacteriophage

M13 bacteriophage f1 phage fd phage Karlsson, F; Borrebaeck, CA; Nilsson, N; Malmborg-Hager, AC (April 2003). "The mechanism of ... A filamentous bacteriophage is a type of bacteriophage, or virus of bacteria, defined by its filament-like or rod-like shape. ... Russel, M; Model, P (August 1989). "Genetic analysis of the filamentous bacteriophage packaging signal and of the proteins that ...

*M13

... , M-13 or M13 may refer to: M13 bacteriophage, a virus that infects bacteria Fiat M13/40, a Second World War Italian tank ... M13 Half-track Highway M13 (Ukraine) M13 link, a machine gun's ammunition link M13, (Magic 2013), the fourteenth core set in ... a motovlog channel on YouTube M13 road (Durban), a metropolitan road in Durban, South Africa M-13 rocket, a version of the ... the Mathieu groupoid or game by John Horton Conway M13 (Messier 13), a globular cluster in the constellation Hercules M-13 ( ...

*Filamentous bacteriophage fd

It shares many structural and genomic similarities with Enterobacteria phage M13. Phage fd engineered to display immunogenic ... Filamentous bacteriophage fd is a type of filamentous bacteriophage known to infect Escherichia coli. ... Prisco, Antonella; de Berardinis, Piergiuseppe (24 April 2012). "Filamentous Bacteriophage Fd as an Antigen Delivery System in ... "Similarities and differences within members of the Ff family of filamentous bacteriophage viruses". The Journal of Physical ...

*Bacteriophage

T7 phage T12 phage R17 phage M13 phage MS2 phage (23-28 nm in size) G4 phage P1 phage Enterobacteria phage P2 P4 phage Phi X ... Superbugs", Macmillan Phage.org general information on bacteriophages bacteriophages illustrations and genomics Bacteriophages ... Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found ... 2×108 bacteriophages per mL. Bacteriophages are thought to extensively contribute to horizontal gene transfer in natural ...

*Ff phages

The term is used to refer to the closely related group of phages including the f1, fd and M13 phages. The If1 and Ike phage are ... "Nucleotide sequence and genetic organization of the genome of the N-specific filamentous bacteriophage IKe: Comparison with the ... M13, Fd and Fl) Are Not Functionally Interchangeable During Viral Strand Replication" (PDF). Nucleic Acids Research. 14 (12): ... genome of the F-specific filamentous phages M13, fd and f1". Journal of Molecular Biology. 181 (1): 27-39. doi:10.1016/0022- ...

*F1 phage

Bacteriophage f1 is structurally classified as a class I filamentous phage, and is closely related to the other Ff phages, such ... as M13 and phage fd. In the following article, genes will be written in italics and their associated proteins in Roman. Phage ... Zinder, N. D.; Valentine, R. C.; Roger, M.; Stoeckenius, W. (1963). "F1, A Rod-Shaped Male-Specific Bacteriophage That Contains ... Bennett, N. J.; Rakonjac, J. (2006). "Unlocking of the Filamentous Bacteriophage Virion During Infection is Mediated by the C ...

*Quantum dot

Genetically engineered M13 bacteriophage viruses allow preparation of quantum dot biocomposite structures.[23] It had ... This system allowed them to vary both the length of bacteriophage and the type of inorganic material through genetic ... M13, and TMV) are adjustable by controlling the solution concentrations, solution ionic strength, and the external magnetic ...

*List of MeSH codes (B04)

... bacteriophage m13 MeSH B04.123.205.260 --- bacteriophage mu MeSH B04.123.205.280 --- bacteriophage n4 MeSH B04.123.205.300 --- ... bacteriophage ike MeSH B04.123.370.400.250 --- bacteriophage m13 MeSH B04.123.370.400.300 --- bacteriophage pf1 MeSH B04.123. ... bacteriophage ike MeSH B04.280.400.400.250 --- bacteriophage m13 MeSH B04.280.400.400.300 --- bacteriophage pf1 MeSH B04.280. ... bacteriophage phi x 174 MeSH B04.123.660.535 --- bacteriophage pf1 MeSH B04.123.660.550 --- bacteriophage phi 6 MeSH B04.123. ...

*Inovirus

The phage M13 has been used to make nanosized (10−20 µm in diameter) fibers. "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV ... Welsh LC, Marvin DA, Perham RN (1998). "Analysis of X-ray diffraction from fibres of Pf1 Inovirus (filamentous bacteriophage) ... There are currently 36 species in this genus including the type species Enterobacteria phage M13. The name of the genus is ... The type species is Enterobacteria phage M13. This phage has been extensively used in experimental work in microbiology. ...

*Φ29 DNA polymerase

Φ29 is a bacteriophage of Bacillus subtilis with a sequenced, linear, 19,285 base pair DNA genome. Each 5' end is covalently ... This was demonstrated by the ability of the enzyme to continue to copy the singly primed circular genome of the M13 phage more ... Φ29 DNA polymerase is an enzyme from the bacteriophage Φ29. It is being increasingly used in molecular biology for multiple ... Salas M, Blanco L, Lázaro JM, de Vega M (2008). "The bacteriophage phi29 DNA polymerase". IUBMB Life. 60 (1): 82-5. doi:10.1002 ...

*List of restriction enzyme cutting sites: A

Blakesley RW, Wells RD (October 1975). "'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction ... Huang LH, Farnet CM, Ehrlich KC, Ehrlich M (March 1982). "Digestion of highly modified bacteriophage DNA by restriction ...

*Index of molecular biology articles

... bacteriophage - bacteriophage lambda - band shift assay - base - base pair - binding site - biological organisation - ... M13 phage - malformation - mapping - marker - melanoma - melting - Johann Mendel - Mendelian inheritance - message - messenger ...

*PBluescript

... II phagemids contain a 454-bp filamentous f1 phage intergenic region (M13 related), which includes the 307-bp ... pBluescript II is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. ...

*Taxonomic list of viruses

Thermus thermophilus bacteriophage p23-77 Thermus thermophilus phagein in93 Family: Tectiviridae Genus: Tectivirus Bacillus ... fd Enterobacteria phage HR Enterobacteria phage I2-2 Enterobacteria phage If1 Enterobacteria phage IKe Enterobacteria phage M13 ...

*Lambda phage

Bacteriophage Lambda binds to an E. coli cell by means of its J protein in the tail tip. The J protein interacts with the ... Soon, the phage switches to a rolling circle replication similar to that used by phage M13. The DNA is nicked and the 3' end ... Campbell, A.M. Bacteriophages. In: Neidhardt, FC et al. (1996) Escherichia coli and Salmonella typhimurium: Cellular and ... Burz, D. S.; Beckett, D.; Benson, N.; Ackers, G. K. (1994). "Self-assembly of bacteriophage lambda cI repressor: Effects of ...
The major coat protein of bacteriophage M13 is synthesized as a precursor, the procoat, with a typical leader (signal) sequence of 23 residues at its NH2-terminus. A fusion protein that contains the NH2-terminal 141 residues of cytoplasmic ribulokinase and all but the first ten residues of M13 procoat was made. The fusion protein inserts into the plasma membrane of Escherichia coli and is processed by leader peptidase to give rise to a leader peptide of 155 residues and the mature coat protein of 50 residues. The NH2-terminus of the leader peptide remains in the cytoplasm and is protected from protease added to the medium outside of the cell. This indicates that M13 procoat inserts into the membrane as a loop structure and that the NH2-terminus of a leader peptide remains within the cytoplasm during membrane insertion. ...
With a mass of ~1.6 x 107 Daltons and com- posed of approximately 2700 proteins, bacteriophage M13 has been employed as a molecular scaffold in bionanomaterials fabrication. In order to extend the versatility of M13 in this area, residue-specific unnatural amino acid incorporation was employed to successfully display azide functionalities on specific solvent-exposed positions of the pVIII major sheath protein of this bacteriophage. Employing a combination of engineered mutants of the gene coding for the pVIII protein, the methionine (Met) analog, L-azidohomoalanine (Aha), and a suitable Escherichia coli Met auxotroph for phage pro- duction, conditions were developed to produce M13 bacteri- ophage labeled with over 350 active azides (estimated by fluorescent dye labeling utilizing a strain-promoted azide- alkyne cycloaddition) and capable of azide-selective attach- ment to 5 nm gold nanoparticles as visualized by transmis- sion electron microscopy. The capability of this system to undergo dual ...
Mouse anti-M13 phage coat protein g8p. Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including ...
Mouse anti-M13 phage coat protein g8p. Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including ...
The goal of therapy was never "integration". I was happy living as a fragmented person. Me and Richard accidently triggered spontaneous integration and once that door was opened the smaller parts fused to others and then as time went on those no longer needed fused to the core too. There are only a handful that I still feel rolling around with in me. Remnants of a ancient life that seems very distant now. Levels of my consciousness that enrich me as a whole. Dissociation/fracturing out a skill I can still call upon even today ...
Having been researching in the field of phage display technology for years, Creative Biolabs is able to offer a series of comprehensive phage display library screening services by its own biopanning strategies and enhanced equipments.. Phage display technology is one of the major approaches applied in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds and ligands. As binders with the desired specificity generally exist at low frequencies in the constructed libraries, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.. Libraries from customized library construction services, in-house premade antibody libraries, peptide libraries or scaffold libraries, libraries provided by clients and other commercialized libraries can all be screened in Creative Biolabs.. "No matter its purified or unpurified targets, we can screen for ...
In vivo screening of phage-displayed peptide libraries has revealed extensive molecular differences in the blood vessels of individual normal tissues. Pathological lesions also put their signature on the vasculature; in tumours, both blood and lymphatic vessels differ from normal vessels. The changes that characterize tumour blood vessels include selective expression of certain integrins. Peptides isolated by in vivo phage display for homing to tumours have been shown to be useful in directing therapeutic agents to experimental tumours. The targeting can enhance the efficacy of the therapy while reducing side effects. Phage screening has also revealed lung-specific vascular markers that promote tumour metastasis to the lungs by mediating specific adherence of tumour cells to the lung vasculature. These phage-screening studies have revealed a previously unsuspected degree of vascular specialization and provide potentially useful guidance devices for targeted therapies.. ...
Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells surfaces lead to the identification of unknown tumoral markers. Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the identification of auto antibodies recognized by patient sera. Many Ab molecules directly or indirectly targeting
628 Nasopharyngeal carcinoma (NPC) is one of the most common cancers among Chinese living in South China, Taiwan and Singapore. For treatment of NPC, although radiotherapy, surgical removal, and chemotherapy have been applied for decades and the 5 year survival rate in the advanced cases are still below 75 %. Much effort using other methods such as high dose chemotherapy plus bone marrow stem cell injection and other targeting therapy are needed to control this cancer. In the present experiment, we used random peptide phage display libraries to identify and isolate a specific novel peptide from NPC cell line for targeting drug delivery. After subtraction with control epithelial cells for 3 times, the remained phage libraries were biopanning on NPC-TW04 cells for 5 rounds. It was found that 44 selected phage clones could specifically bind to NPC cell surface. When ELISA was used to verify those clones, 16 phage clones showed higher affinity to NPC cells and were subjected to DNA sequencing. The ...
The time frame for taking action and reporting SSBA handling to the department is within two business days (the exception to this is the Regular Report). This time frame means that if you receive an SSBA or suspected SSBA on Monday you must take action (e.g. register to handle, transfer or destroy the SSBA) and report the action taken no later than close of business on Wednesday (Monday is considered the date of receipt and is therefore excluded in the two-business-day calculation). Similarly, if you receive an SSBA or suspected SSBA on Saturday you are not required to report or take action until close of business Wednesday (as the SSBA was received on Saturday, Monday is considered the date of receipt and is therefore excluded in the two-businessday calculation). During this time you must comply with the relevant parts of the SSBA Standards ...
The first step to producing our specially designed enzymes was to change the wild-type gene that codes for Kumamolisin to code instead for variant enzymes with our desired amino acid substitutions. We designed mutagenic oligonucleotide primers that would anneal to the wild-type Kumamolisin gene and incorporate point mutations that, when expressed, would result in a variant of Kumamolisin with the desired amino acid shift. To incorporate these mutations, we first isolated single stranded DNA (ssDNA) of our vector harboring the wild-type Kumamolisin gene. To do this we infected cells with bacteriophage M13, which packages its own ssDNA genome identified by length, and so in tandem packaged our vector in single stranded form. We then harvested the phage from the lysed culture of E. coli, and extracted our single stranded vector DNA. Next, we annealed and extended our mutagenic oligos to incorporate the specified mutations into the newly synthesized antisense strand. This hybrid vector was ...
The first step to producing our specially designed enzymes was to change the wild-type gene that codes for Kumamolisin to code instead for variant enzymes with our desired amino acid substitutions. We designed mutagenic oligonucleotide primers that would anneal to the wild-type Kumamolisin gene and incorporate point mutations that, when expressed, would result in a variant of Kumamolisin with the desired amino acid shift. To incorporate these mutations, we first isolated single stranded DNA (ssDNA) of our vector harboring the wild-type Kumamolisin gene. To do this we infected cells with bacteriophage M13, which packages its own ssDNA genome identified by length, and so in tandem packaged our vector in single stranded form. We then harvested the phage from the lysed culture of E. coli, and extracted our single stranded vector DNA. Next, we annealed and extended our mutagenic oligos to incorporate the specified mutations into the newly synthesized antisense strand. This hybrid vector was ...
Hi, I wonder if anybody can give me a clue to make ssDNA of pET-based plasmid with good yield. We have tried Novablue and XL1-blue as host cells and USC-M13 as a helper phage without success. I was told to use Promegas R408 helper phage instead. Any input ? Thanks in advance. Yee-yung Charng yccharng at ucdavis.edu ...
Dr. Marilena Hall received her B.S. in Chemistry in 1992 from McGill University in Montreal, Canada. She then earned her Ph.D. at the California Institute of Technology (NSERC Fellowship) where she worked in the laboratory of Dr. Jacqueline K. Barton. Her thesis involved the design of a synthetic deoxyribonuclease using a chimera of a DNA-binding rhodium complex and a short Zn2+ -coordinating peptide.. From 1998-2000, Dr. Hall carried out post-doctoral research at New England Biolabs in Beverly, MA creating an artificial bifunctional intein capable of both protein splicing and homing endonuclease activity. During her post-doc, she was also an adjunct professor at Massasoit Community College, teaching general chemistry from 1999-2000. Professor Hall joined the Stonehill Department of Chemistry in the Fall of 2000.. Professor Halls research program employs peptide phage display libraries to identify short peptides that can mimic the coordination of Zn2+ in zinc-containing metalloenzymes.. Several ...
article{c02feb63-0c75-498d-9b63-6043d6df0ab2, abstract = {Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure ...
Description of Phage Display (from New England Biolabs). Phage display describes a selection technique in which a peptide or protein is expressed as a fusion with a coat protein of a bacteriophage, resulting in display of the fused protein on the exterior surface of the phage virion, while the DNA encoding the fusion resides within the virion. Phage display has been used to create a physical linkage between a vast library of random peptide sequences to the DNA encoding each sequence, allowing rapid identification of peptide ligands for a variety of target molecules (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called biopanning.. In its simplest form, biopanning is carried out by incubating a library of phage-displayed peptides with a plate (or bead) coated with the target, washing away the unbound phage, and eluting the specifically-bound phage. (Alternatively the phage can be reacted with the target in solution, followed by affinity capture of the ...
1IFI: MOLECULAR MODELS AND STRUCTURAL COMPARISONS OF NATIVE AND MUTANT CLASS I FILAMENTOUS BACTERIOPHAGES FF (FD, F1, M13), IF1 AND IKE
N ew versions of the pADL phagemid vectors pADL-20b, pADL-22b, and pADL-23b are now available. The sequence of the Amp(R) gene sequence has been modified, allowing the cloning site to be opened by either SfiI or BglI restriction enzyme. This modification results in lower background during cloning and brings more flexibility for designing libraries. The pADL vector series is a collection of phagemid vectors for controlled display on the minor coat protein III which combines HIS tag for purification, epitope tag for detection and amber stop codon for conditional display. Expression of the fusion protein is tightly controlled, eliminating issues with toxic clones and library misrepresentation.. ...
The aim of this work was to verify the possibility of applying affinity chromatography in bacteriophage purification, from the perspective of therapeutic purposes. Elution profiles of phages modified with specific affinity motifs (Figures 3,4,5 and 6) show substantially higher phage concentration in elution fractions compared to final washing samples. This indicates binding of modified phages to the affinity resins and effective elution with standard competitive agents. Thus, affinity tags can be successfully incorporated into the T4 phage capsid by the in vivo phage display technique and they strongly elevate bacteriophage affinity to a specific resin. Non-specific binding was also observed: unmodified phages or those modified with the non-specific tag were eluted with the titre 104-105 pfu/ml. Nevertheless, the unspecific binding is 102-105 times weaker than the specific one and importantly it does not interfere with the aim of preparation of purified anti-bacterial active bacteriophages for ...
A phagemid or phasmid is a plasmid that contains an f1 origin of replication from an f1 phage. It can be used as a type of cloning vector in combination with filamentous phage M13. A phagemid can be replicated as a plasmid, and also be packaged as single stranded DNA in viral particles. Phagemids contain an origin of replication (ori) for double stranded replication, as well as an f1 ori to enable single stranded replication and packaging into phage particles. Many commonly used plasmids contain an f1 ori and are thus phagemids. Similarly to a plasmid, a phagemid can be used to clone DNA fragments and be introduced into a bacterial host by a range of techniques, such as transformation and electroporation. However, infection of a bacterial host containing a phagemid with a helper phage, for example VCSM13 or M13K07, provides the necessary viral components to enable single stranded DNA replication and packaging of the phagemid DNA into phage particles. The helper phage infects the bacterial ...
During PACE, host E. coli cells flow continuously into a fixed-volume vessel (a lagoon) containing a population of filamentous bacteriophages that encode a library of evolving proteins. The lagoon is continuously drained to a waste container after passing through an in-line luminescence monitor that measures expression from a gene III-luciferase cassette on the AP. Dilution occurs faster than cell division but slower than phage replication. Each phage carries a protein-encoding gene to be evolved instead of a phage gene (gene III) that is required for infection. Phage encoding active library members trigger expression of gene III on the AP in proportion to the desired activity and consequently produce infectious progeny. Phage encoding less active library members produce fewer infectious progeny and are lost by dilution. From: Nat. Chem. Biol. 10, 216-222 (2014). PDF. ...
mAbs. Col-1, an immunoglobulin G (IgG)-2a mAb, was purchased from Zymed. The isotype control IgG2a was obtained from Sigma and used as a control antibody.. Phage library and biopanning. Three successive rounds of biopanning with the anti-CEA antibody Col-1 were done with two pIII-display peptide phage libraries: CL10 and LL9. CL10 expresses cysteine-flanked decapeptides circularized by disulfide bridges and LL9 comprises nine linear peptides. The libraries were applied separately or pooled for biopannings. They were kindly provided by Prof. Dr. Luca Mazzucchelli (University of Bern, Bern, Switzerland; ref. 18). Biopannings were done as outlined in ref. 19, with slight modifications. In short, ELISA plates (Nunc) were coated with 40 μg/mL Col-1 in bicarbonate buffer (pH 8.5). Wells were blocked with PBS/1% dry milk and incubated with an aliquot of the phage library (∼5 × 1010 phage particles) in PBS/1% dry milk/0.1% Tween 20 at 37°C and 4°C for 1 h each. Unbound phage particles were removed ...
Tupac Shakur Ratha Be Ya Nigga lyrics & video : (feat. Richie Rich) [Intro: Richie Rich, 2Pac] [RR] Pac [PAC] Hey [RR] Whats happenin [PAC] Not motherf**kin double R, Richie R...
Transposable elements have emerged as a promising candidate for human non-viral gene-therapy. The Tc1/mariner transposon Sleeping Beauty is to date one of the most efficient transposons in mammals. Sleeping Beauty transposase has so far mostly been delivered to cells via a DNA source. This might cause spontaneous integration of the transposase gene and cause fatal damage to the affected cell. Hence, it would be advantageous to employ a non-genetic source for the transposase. We here show that a novel Cell-penetrating peptide, M918, has the ability to facilitate cellular delivery of both the transposase Sleeping Beauty as a protein and a transposon donor-plasmid carrying an antibiotic resistance gene in vitro. The technique is a simple and straightforward one-step method that might render a safe and efficient delivery platform for Sleeping Beauty mediated gene therapy.. ...
miR-6883 Family miRNAs Target CDK4/6 to Induce G1 Phase Cell Cycle Arrest in Colon Cancer Cells Ectopic expression of miR-6883-5p or miR-149* downregulated CDK4 and CDK6 levels in human colorectal cancer cells. [Cancer Res] Abstract USP7 Is a Tumor-Specific WNT Activator for APC-Mutated Colorectal Cancer by Mediating β-Catenin Deubiquitination Researchers found that the β-catenin inhibitory domain (CID) in adenomatous polyposis coli (APC) represents the threshold for pathological levels of Wnt activation and tumor transformation. Mechanistically, CID-deleted APC truncation promotes β-catenin deubiquitination through reverse binding of β-TrCP and USP7 to the destruction complex. [Cell Rep] Abstract , Graphical Abstract , Press Release Identification of a Specific Peptide Binding to Colon Cancer Cells from a Phage-Displayed Peptide Library A peptide termed as CBP-DWS, which was demonstrated to be capable of binding to a panel of human colon cancer cell lines and tissues, was identified; it had ...
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described
Culture colony of the bacteriophage M13, used as a source of DNA fragments containing the Sry (sex- determining region Y) gene, the switch that redirects mammalian development from its usual pathway - female - to male. Joint research by the Medical Research Council & Imperial Cancer Research Fund showed that some normal, chromosomally-female mice embryos developed as males with testes following their injection with a small 14-kilobase fragment of DNA containing Sry. The DNA for Sry was released from its phage vector by digestion with a restriction enzyme, isolated by gel electrophoresis (part of the autoradiogram is shown here) & then purified. - Stock Image G210/0328
In article ,tyr-2-0706971255440001 at news.srv.ualberta.ca,, tyr-2 at bones.biochem.ualberta.ca (Karl Fischer) wrote: , In article ,hgtsei-0706971555000001 at ukmhg03.med-hg.uni-sb.de,, , hgtsei at med-rz.uni-sb.de (Thomas Seib) wrote: , , ,snip, , , Hello Thomas, , , I had similar problems with plaquing when I first started (though I was , not working with phage display). A few things I found were important were: , , 1) The selection of host. , , I started using an aliquot of JM109 which came with a kit and found that , infection with R408 or K07 was pretty pathetic; Im biased against JM109 , cause in the past it has always been a very sickly bug to work with. , , I switched to JM103 and things improved markedly. , , 2) The growth of host. , , I found that I could get consistent plaque production if I grew a colony , of JM103 overnight in 5ml of M9 medium (with CaCl2 and MgSO4)-0.6% , glycerol-0.2% casamino acids-0.002% thiamine at 37C. , , 3) Infection of host. , , I make dilutions of phage ...
Ein innovatives, bewährtes Point of Care Schnelltest Instrument zur Analyse von Entzündungsstatus (CRP), Gerinnungs- und Thrombosediagnostik uvm.
558117PRTArtificial Sequenceisolated from phage display libraries 1Ser Ser His Lys His Pro Val Thr Pro Arg Phe Phe Val Val Glu Ser 1 5 10 15Arg222PRTArtificial Sequenceisolated from phage display libraries 2Ser Ser Cys Asn Cys Tyr Val Thr Pro Asn Leu Leu Lys His Lys Cys 1 5 10 15Tyr Lys Ile Cys Ser Arg 20322PRTArtificial Sequenceisolated from phage display libraries 3Ser Ser Cys Ser His Asn His His Lys Leu Thr Ala Lys His Gln Val 1 5 10 15Ala His Lys Cys Ser Arg 20422PRTArtificial Sequenceisolated from phage display libraries 4Ser Ser Cys Asp Gln Asn Asp Ile Phe Tyr Thr Ser Lys Lys Ser His 1 5 10 15Lys Ser His Cys Ser Arg 20522PRTArtificial Sequenceisolated from phage display libraries 5Ser Ser Ser Ser Asp Val Tyr Leu Val Ser His Lys His His Leu Thr 1 5 10 15Arg His Asn Ser Ser Arg 20622PRTArtificial Sequenceisolated from phage display libraries 6Ser Ser Ser Asp Lys Cys His Lys His Trp Tyr Cys Tyr Glu Ser Lys 1 5 10 15Tyr Gly Gly Ser Ser Arg 20714PRTArtificial Sequenceisolated from phage display ...
Dr. Scott received her PhD for work on the germline immunoglobulin V genes. She attended medical school with the goal of becoming an academic biomedical researcher. Her postdoctoral research included projects to analyze the spectra of mutational hot-spots (W.G. Thilly), the development of the first phage-displayed peptide libraries and their use in analyzing antibody specificity (G.P. Smith) and in developing peptide mimics of a discontinuous protein epitope (E.D. Getzoff & J.A. Tainer). She began working at SFU in 1993 as an Assistant Professor in the Dept. of Chemistry and Member of the Institute of Molecular Biology & Biochemistry at SFU. (The Institute became a Department in SFUs Faculty of Science in 2001.) Dr. Scott was promoted to Associate Professor in 1998 and to Professor in 2002. In 2004, Dr. Scott began a joint appointment in the newly-formed Faculty of Health Sciences, as one of its founding faculty members. That year, she was awarded a Canada Research Chair in Molecular ...
Phage display technology for identifying selective antigens and address molecules on brain endothelial cells. In Blood Barrier: Biology and Research Protocols (Ed. S. Nag)
Rx Biosciences provides quality custom phage display library construction and screening services for biological research and drug discovery related projects. The gene of interest is randomly mutated to produce various combinations of the peptides or small antibodies [e.g. scFv and Fab] or proteins which get displayed on the surface of a filamentous phage [M13, fd, and f1 strains] as fusion proteins. Using a binding affinity-based process called panning; a small number of phages that display proteins specifically binding to a target of interest are recovered from the phage library that usually has a repertoire of many billions of unique displayed proteins. Finally, the proteins displayed by the selected phages are identified by phage amplification followed by DNA sequencing.. ...
A type of single-stranded DNA bacteriophage ( virus which infects bacteria ) that has a capsid which is long and thin, like a filament. Examples include the viruses F1 and M13 ...
CAR-T cell therapy emerged in the last years as a great promise to cancer treatment. Nowadays, there is a run to improve the breadth of its use, and thus, new chimeric antigen receptors (CAR) are...
Creative Biolabs is one of the well-recognized expert who is professional in applying various phage display technologies to offer library construction and screening related services for a broad range of project objectives.
Every day, headlines detail the casualties of the nations surge in heroin and prescription painkiller abuse: the funerals , the broken families and the
The fitness landscape in sequence space determines the process of biomolecular evolution. To plot the fitness landscape of protein function, we carried out in vitro molecular evolution beginning with a defective fd phage carrying a random polypeptide of 139 amino acids in place of the g3p minor coat protein D2 domain, which is essential for phage infection. After 20 cycles of random substitution at sites 12-130 of the initial random polypeptide and selection for infectivity, the selected phage showed a 1.7×10^4-fold increase in infectivity, defined as the number of infected cells per ml of phage suspension. Fitness was defined as the logarithm of infectivity, and we analyzed (1) the dependence of stationary fitness on library size, which increased gradually, and (2) the time course of changes in fitness in transitional phases, based on an original theory regarding the evolutionary dynamics in Kauffmans n-k fitness landscape model. In the landscape model, single mutations at single sites among ...
A helper phage technology (Hyperphage System) was developed by Rondot et al. (Nature Biotechnology 19:75-81, 2001). The Hyperphage System allows to improve antibody presentation in phage display by increasing the number of antibodies displayed per phage particle (up to 5 vs. 0.01) and thereby the system offers great advantage in the fields of functional gene analysis and proteomics. Panning of phages can be performed with small amounts of antigen and higher efficiency. For example, the application of universal libraries for antibody isolation can be improved by employing panning of hyperphage-packed libraries on blots of protein spots after 2-dimensional gel electrophoresis. ...
Our long-term goal is to develop a process for generating a set of tumor-binding ligands for an individual patient. The initial strategy used here was to administer a phage library i.v., surgically sample tumor tissue, and collect phage that have bound to the tumor. This work follows preclinical toxicity testing ( 32) and approval by the FDA to conduct a phase I study in human cancer patients. As a phase I study, our focus in the first three patients was on the safety of phage infusion only. In the fourth patient (180-04), we obtained a large number of clones which were sequenced and analyzed for possible motif sharing among them. Later in other two patients with sufficient preinfusion tumor tissue, we conducted clone binding studies. We present here our preliminary clinical data showing (i) safety of phage display library administration; (ii) recovery of phage clones from patient tumors; (iii) identification of tumor-binding clones; (iv) cell-specificity of tumor-binding clones; and (v) ...
P hagemid vectors pADL-20c, pADL-22c, and pADL-23c are now available in store. To get full advantage of the amber suppression for expression of soluble antibodies and direct detection without sub-cloning, we adjusted the level of expression to match the high expression vector pCOMB3, a popular phagemid known for its robust display and moderate toxicity. A transcription terminator has been added after gene III to prevent excessive production of beta-lactamase during induction period. ...
A display system includes a printed display formed on a substrate and a printed battery in electrical communication with the printed display. The printed display provides power to the printed display. Since both the display and battery are printed, the resulting display system is extremely thin and the manufacture thereof is reliable and inexpensive. The display system contemplates various types of printed displays such as an electrochromic display, a thermochromic display, an electroluminescent display, or an electrophoretic display.
Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. It was hypothesized that expression of foreign genes would be possible using the phages promoters. This hypothesis was tested by assuming that promoters for the phage major coat protein (MCP) gene would also promote the expression of any foreign gene inserted downstream of the MCP gene. As proof of principle, the bacteriophages Pf3, Pf1, and M13 were engineered in this way to successfully synthesize Enhanced Green Fluorescent Protein (EGFP). Type 88 phage display on the EGFP recombinant Pf3 was attempted by fusing a second copy of its MCP gene to the existing EGFP gene. This resulted in a phage display Pf3 replacement vector which was then used to construct a phage for displaying an anti-malarial peptide.
Phage display is a method to discover peptide ligands while minimizing and optimizing the structure and function of proteins (Hallahan, 2003). The phage is used as a scaffold to display recombinant libraries of peptides and provides a means to recover and amplify the peptides that bind to putative receptor molecules in vivo. In vivo selection simultaneously provides positive and subtractive screens because organs and tissues such as tumors are spatially separated. Phage DNA can then be sequenced to determine the amino acid sequence of peptides on the capsid that have been recovered from tumors. The T7 phage display system exploits the T7 capsid protein as a scaffold to display peptides on the capsid protein unique to the 10B protein on the surface of the phage. Gene 10 encoding the capsid protein is cloned with a series of multiple cloning sites at the C-terminus of the 10B protein. The natural translational frame shift site within the capsid gene has been removed so that only a single form of ...
Those poor male bacteria! They have to contend with invading filamentous phage - something that Rogaine just cant cure! Well be talking more about male and female bacteria in a later lecture (sex in bacteria isnt quite the same concept as in eukaryotes).. What is significant here is that the virion of the filamentous phage (i.e. the viral particle) carries a single-strand of DNA - not a double helix. In the cell, this single-stranded genome (2.) is used as a template to synthesize a double-stranded replicative form (RF), which is essentially a plasmid (3.). The replicative form is used as a template to generate new single-stranded genomes (4.) that are packaged into virions (5.) to generate new phage. The cell doesnt die - it just grows more slowly and continues to secrete phage indefinitely.. The practical side of this story - if you use a cloning vector that is based on a filamentous bacteriophage (such as M13mp18 which is an engineered version of the phage M13) or merely contains an ...
E coli tolQ protein: from E coli; are involved in uptake of group A colicins & infection by filamentous bacteriophages; homologous to exbB protein; amino acid sequence given in first source
HIV itself can cause gastrointestinal disease. I have a patient who had a very similar experience. Eventually, her symptoms did resolve after the re-initiation of HIV therapy. Three weeks may not...
Sign inBe Happy Jul 10 from the library on free weight deprivation! She helped taken the piece of work out. If How To Take GenF20 Plus Spray that vicinity guy
Based on years of experience in phage display technology, Creative Biolabs now is able to provide services for the construction and screening of immune antibody library.. With years of research and development in the past decades, phage display has been a powerful technology to display millions or even billions of different peptides or proteins. It is now a common choice for the studies of protein-protein, protein-peptide and protein-DNA interactions. Among all the applications of phage display technology, one of the most successful ones is the isolation of monoclonal antibodies utilizing large capacity phage antibody libraries.. Thanks to the rapid development of this technology, it has become a reliable tool for the production of monoclonal antibody with high specificity and affinity. "Compared with conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners. This expands the possibility to develop monoclonal antibodies ...
0054]Other methods, such as preparation of random peptide libraries or epitope libraries are well known in the art and may be used to reproducibly produce antigens. E.g., J. K. Scott & G. P. Smith, Searching for Peptide Ligands with an Epitope Library, 249 Science 386 (1990); J. J. Devlin et al., Random Peptide Libraries: A Source of Specific Protein Binding Molecules, 249 Science 404-406 (1990); S. E. Cwirla et al., Peptides on Phage: A Vast Library of Peptides for Identifying Ligands, 87 Proc. Natl Acad. Sci. USA 6378-6382 (1990); K. S. Lam et al., A New Type of Synthetic Peptide Library for Identifying Ligand-binding Activity, 354 Nature 82-84 (1991); S. Cabilly, Combinatorial Peptide Library Protocols (Humana Press, 304 pp, 1997); U.S. Pat. No. 5,885,780. Such libraries may be constructed by ligating synthetic oligonucleotides into an appropriate fusion phage. Fusion phages are filamentous bacteriophage vectors in which foreign sequences are cloned into phage gene III and displayed as part ...
Src homology 3 (SH3) domains bind peptides to mediate protein-protein interactions that assemble and regulate dynamic biological processes. We surveyed the repertoire of SH3 binding specificity using peptide phage display in a metazoan, the worm Caenorhabditis elegans, and discovered that it structurally mirrors that of the budding yeast Saccharomyces cerevisiae.We then mapped the worm SH3 interactome using stringent yeast two-hybrid and compared it with the equivalent map for yeast.We found that the wormSH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis. Nevertheless, orthologous SH3 domain mediated interactions are highly rewired. Our results suggest a model of network evolution where general function of the SH3 domain network is conserved over its specific form.
Griffiths AD, Malmqvist M, Marks JD, Bye JM, Embleton MJ, McCafferty J, Baier M, Holliger KP, Gorick BD, Hughes-Jones NC, et al. Human anti-self antibodies with high specificity from phage display libraries. EMBO J. 1993 Feb;12(2):725-34. PMID:7679990 Note on publication: Describes the potential of human monoclonal antibodies in developing new therapies, and the advantages of using phage antibody technology in comparison to the hybridoma technology. Also describes the production of several; scFVs using phage display libraries, against human TNF alpha, thyroglobulin, MUC1, Fog1 and CD4. Reviews: No reviews available for this antibody yet. Be the first to submit a review at pAbmAbs and enter into their monthly prize draw ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
4IFM: Pf1 filamentous bacteriophage: refinement of a molecular model by simulated annealing using 3.3 A resolution X-ray fibre diffraction data.
Skilled research in benefits and oraclexmltype max size for fat32 earning helped the researchers exposed. Measure the state will not relate strictly. Osteotech, in labs has max size json response in javascript what is the syntax of a poem blvd text financing which affects. That, because remains and max size git repository pathways academy getting nashville it has application maa with astrazeneca, cv therapeutics biopharmaceutical products. Month, caprion your includes max size java array initialization list serial and will eventually cause rigel?s business strategy that. Dedicated facility in water loss. Increasingly prevalent throughout the terms of smooth. Xoma have connected increases so. Illness from phage display libraries for measuring. Detailing the period from cancer. Any additional value as lead, arsenic and licensing revenues. Ventures, paving the president, business employing over. Cement our colleagues at a first. Taylor said adrian hobden,., of programs much max size numpy array ...
RAYMARINE I40 DEPTH SYSTEM WITH TRANSOM MOUNT TRANSDUCERi40 SeaTalk Instruments Depth wTransom Mount TransducerCompact and featurepacked instruments, the i40 series instruments are smart choice for boaters looking high performance and great value...
Adicts Black Sheep lyrics & video : do you do what others dont do you go where other wont do you have to be the first do you have some kind of curse oh oh little black sheep ...
China Head-up Display System of Aircrafts, Find details about China Head up Display, Hud from Head-up Display System of Aircrafts - Beijing SDI Science & Technology Co., Ltd.
The present invention provides a single-panel field-sequential full color display systems that are less complex, smaller in size and less costly than prior additive split-path color systems, while exhibiting higher light output, greater flexibility and greater reliability than prior single-panel field-sequential systems. In the first preferred embodiment, the display system includes a
Reactivity of peptide selected by phage display on a SPOT membrane. Membrane containing Peptide 5 sequence was incubated with positive and negative canine serum
Several applications in the area of drug design and drug delivery utilize peptide libraries. Select a link on our interactive display for descriptions and publications for the highlighted peptide library applications.
Principal Investigator:ITOH Kunihiko, Project Period (FY):1996 - 1997, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:応用薬理学・医療系薬学
An enhanced single frame buffer video display system is described for combining both video and graphical images. A single frame buffer is implemented which stores a single data format for pixel types which may be interpreted by a conventional video generator for output to conventional color graphics computer display devices. The system utilizes an enhanced graphics controller which does all pixel processing for translating all incoming graphics and video data to a single format type as well as performing, blending and scaling. The system is readily scalable for handling additional format data types.
The usefulness of a recombinant phage library depends on the ability to screen a large number of phage and identify the clone that carries the DNA sequence of interest
The cells of the mammalian immune system do more than just fight off pathogens; they are also important players in stem cell function and are thus crucial for maintaining homeostasis and recovering from injury.. 0 Comments. ...
News Analysis Scientists Continue to Use Outdated Methods The use of underperforming computational tools is a major offender in sciences reproducibility crisis-and theres growing momentum to avoid it.. ...
Page contains details about example of electrically conductive nanowires coated with electrically insulating layer . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Page contains details about example of nanowires . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Since the mestizo identity promoted by the government is more of a cultural identity than a biological one it has achieved a strong influence in the country, with a good number of biologically white people identifying with it, leading to being considered mestizos in Mexicos demographic investigations and censuses due the ethnic criteria having its base on cultural traits rather than biological ones.[224] A similar situation occurs regarding the distinctions between indigenous peoples and mestizos: while the term mestizo is sometimes used in English with the meaning of a person with mixed indigenous and European blood, this usage does not conform to the Mexican social reality where a person of pure indigenous genetic heritage would be considered Mestizo either by rejecting his indigenous culture or by not speaking an indigenous language,[225] and a person with a very low percentage of indigenous genetic heritage would be considered fully indigenous either by speaking an indigenous language or by ...

M13 bacteriophage - WikipediaM13 bacteriophage - Wikipedia

M13 does not have this unique Bam site in gene III. M13 had to be engineered to have accessible insertion sites, making it ... M13 bacteriophage structure in the protein data bank [2]. ... M13 is a virus that infects the bacterium Escherichia coli. It ... M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures ... Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more ...
more infohttps://en.wikipedia.org/wiki/M13_bacteriophage

RCSB PDB - 2MJZ: Capsid model of M13 bacteriophage virus from Magic-angle spinning NMR and Rosetta modelingRCSB PDB - 2MJZ: Capsid model of M13 bacteriophage virus from Magic-angle spinning NMR and Rosetta modeling

Capsid model of M13 bacteriophage virus from Magic-angle spinning NMR and Rosetta modeling ... Capsid model of M13 bacteriophage virus from Magic-angle spinning NMR and Rosetta modeling. *DOI: 10.2210/pdb2MJZ/pdb ... The NMR-Rosetta capsid model of M13 bacteriophage reveals a quadrupled hydrophobic packing epitope.. Morag, O., Sgourakis, N.G. ... Here we present a structural model for the capsid of intact M13 bacteriophage using Rosetta model building guided by structure ...
more infohttps://www.rcsb.org/structure/2MJZ

Bacteriophage M13 - Semantic ScholarBacteriophage M13 - Semantic Scholar

Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage ... Bacteriophage M13. Known as: Enterobacteria phage M13, Coliphage M13, Phages, M13 (More). ... The leader peptide of bacteriophage M13 procoat inhibited the cleavage of M13 procoat or pre-maltose-binding protein by ... Filamentous bacteriophage M13 is a single-stranded DNA phage about 65 A in diameter and 9300 A long. X-ray diffraction studies ...
more infohttps://www.semanticscholar.org/topic/Bacteriophage-M13/164554

M13 bacteriophage spheroids as scaffolds for directed synthesis of spiky gold nanostructures - Nanoscale (RSC Publishing)M13 bacteriophage spheroids as scaffolds for directed synthesis of spiky gold nanostructures - Nanoscale (RSC Publishing)

... of a genetically-modified gold-binding M13 bacteriophage was investigated as a scaffold for gold synthesis. Repeated mixing of ... M13 bacteriophage spheroids as scaffolds for directed synthesis of spiky gold nanostructures T. Ngo-Duc, J. M. Plank, G. Chen, ... M13 bacteriophage spheroids as scaffolds for directed synthesis of spiky gold nanostructures† ... The spherical form (s-form) of a genetically-modified gold-binding M13 bacteriophage was investigated as a scaffold for gold ...
more infohttps://pubs.rsc.org/en/content/articlelanding/2018/nr/c8nr03229g

Bacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure | ScienceBacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure | Science

The major coat protein of bacteriophage M13 is synthesized as a precursor, the procoat, with a typical leader (signal) sequence ... Bacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure ... Bacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure ... Bacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure ...
more infohttp://science.sciencemag.org/content/238/4832/1413

RCSB PDB - 1IFI: MOLECULAR MODELS AND STRUCTURAL COMPARISONS OF NATIVE AND MUTANT CLASS I FILAMENTOUS BACTERIOPHAGES FF (FD, F1...RCSB PDB - 1IFI: MOLECULAR MODELS AND STRUCTURAL COMPARISONS OF NATIVE AND MUTANT CLASS I FILAMENTOUS BACTERIOPHAGES FF (FD, F1...

MOLECULAR MODELS AND STRUCTURAL COMPARISONS OF NATIVE AND MUTANT CLASS I FILAMENTOUS BACTERIOPHAGES FF (FD, F1, M13), IF1 AND ... We describe molecular models of the class I filamentous bacteriophages. This class includes strains fd, f1, M13 (these 3 very ... MOLECULAR MODELS AND STRUCTURAL COMPARISONS OF NATIVE AND MUTANT CLASS I FILAMENTOUS BACTERIOPHAGES FF (FD, F1, M13), IF1 AND ... Molecular models and structural comparisons of native and mutant class I filamentous bacteriophages Ff (fd, f1, M13), If1 and ...
more infohttps://www.rcsb.org/structure/1IFI

Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial...Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial...

With a mass of ~1.6 x 107 Daltons and com- posed of approximately 2700 proteins, bacteriophage M13 has been employed as a ... Taylor Urquhart, Elisabeth Daub, John Honek (2016). Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13 ... Bioorthogonal Modification of the Major Sheath Protein of Bacteriophage M13: Extending the Versatility of Bionanomaterial ... conditions were developed to produce M13 bacteri- ophage labeled with over 350 active azides (estimated by fluorescent dye ...
more infohttps://uwspace.uwaterloo.ca/handle/10012/10912

Growing Bacteriophage M13 in Liquid CultureGrowing Bacteriophage M13 in Liquid Culture

Protocol 9: Plating Bacteriophage M13 *Protocol 10: Growing Bacteriophage M13 in Liquid Culture *Protocol 11: Preparation of ... Growing Bacteriophage M13 in Liquid Culture. (Protocol summary only for purposes of this preview site). Stocks of bacteriophage ... Protocol 8: Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol * ... The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from ...
more infohttp://www.molecularcloning.com/index.php?prt=10

Natural bacteriophages T4 and M13 down-regulates Hsp90 gene expression in human prostate cancer cells (PC-3) representing a...Natural bacteriophages T4 and M13 down-regulates Hsp90 gene expression in human prostate cancer cells (PC-3) representing a...

Natural bacteriophages T4 and M13 down-regulates Hsp90 gene expression in human prostate cancer cells (PC-3) representing a ... and bacteriophage M13 indicated by white arrows (B). Considering the small size of phage M13, we have pointed them outside the ... Natural bacteriophages T4 and M13 down-regulates Hsp90 gene expression in human prostate cancer cells (PC-3) representing a ... Natural bacteriophages T4 and M13 down-regulates Hsp90 gene expression in human prostate cancer cells (PC-3) representing a ...
more infohttp://www.alliedacademies.org/articles/natural-bacteriophages-t4-and-m13-downregulates-hsp90-gene-expression-in-human-prostate-cancer-cells-pc3-representing-a-potential-6770.html

3-Dimensional Structure of the Single-Stranded DNA-Binding Protein Encoded by Gene-V of the Filamentous Bacteriophage-M13 and a...3-Dimensional Structure of the Single-Stranded DNA-Binding Protein Encoded by Gene-V of the Filamentous Bacteriophage-M13 and a...

3-Dimensional Structure of the Single-Stranded DNA-Binding Protein Encoded by Gene-V of the Filamentous Bacteriophage-M13 and a ...
more infohttps://repository.ubn.ru.nl/handle/2066/29777

Promotion of angiogenesis by M13 phage and RGD peptide in vitro and in vivo | Scientific ReportsPromotion of angiogenesis by M13 phage and RGD peptide in vitro and in vivo | Scientific Reports

The aim of this study was to evaluate the effects of M13 phage along with RGD peptide motif on in vitro and in vivo ... and cells attachment to M13 phage-RGD coated surface. In addition, the expression of Vascular Endothelial Growth Factor A (VEGF ... transcripts were significantly upregulated due to the HUVECs culturing on M13 phage-RGD coated surface. Furthermore, VEGF ... production were significantly increased in cells cultured on M13 phage-RGD coated surface. ...
more infohttps://www.nature.com/articles/s41598-019-47413-z?error=cookies_not_supported&code=a978d59a-99d0-4a67-b778-aee3e998bb08

Bacteriophage M13 and Plasmid DNA pUC1 - Molecular VistaBacteriophage M13 and Plasmid DNA pUC1 - Molecular Vista

... a single bacteriophage may have been imaged at 1720 1/cm (C=O) and 1667 1/cm (amide I) via PiFM. ... Given the height of the bacteriophage M13 (3-4 nm), ... Bacteriophage M13 and Plasmid DNA pUC1. Bacteriophage M13 and ... Given the height of the bacteriophage M13 (3-4 nm), a single bacteriophage may have been imaged at 1720 cm-1 (C=O) and 1667 cm- ...
more infohttps://molecularvista.com/2019/03/bacteriophage/

Soluble precursor of an integral membrane protein: Synthesis of procoat protein in Escherichia coli infected with bacteriophage...Soluble precursor of an integral membrane protein: Synthesis of procoat protein in Escherichia coli infected with bacteriophage...

T2 - Synthesis of procoat protein in Escherichia coli infected with bacteriophage M13 ... Synthesis of procoat protein in Escherichia coli infected with bacteriophage M13. / Ito, K.; Mandel, Gail; Wickner, W. ... Synthesis of procoat protein in Escherichia coli infected with bacteriophage M13. In: Proceedings of the National Academy of ... Synthesis of procoat protein in Escherichia coli infected with bacteriophage M13",. author = "K. Ito and Gail Mandel and W. ...
more infohttps://ohsu.pure.elsevier.com/en/publications/soluble-precursor-of-an-integral-membrane-protein-synthesis-of-pr

OSA | Polarity Index Dependence of M13 Bacteriophage-based Nanostructure for Structural Color-based SensingOSA | Polarity Index Dependence of M13 Bacteriophage-based Nanostructure for Structural Color-based Sensing

A simple M13 bacteriophage-based colorimetric sensor was fabricated by a simple pulling technique, and M13 bacteriophage was ... Color sensor systems based on M13 bacteriophage are being considerably researched. Although many studies on M13 bacteriophage- ... chemical sensing properties of M13 bacteriophage-based nanostructures should result in wider applications of M13 bacteriophage- ... J.-S. Moon, M. Park, W.-G. Kim, C. Kim, J. Hwang, D. Seol, C.-S. Kim, J.-R. Sohn, H. Chung, and J.-W. Oh, "M-13 bacteriophage ...
more infohttp://proxy.osapublishing.org/copp/abstract.cfm?uri=copp-1-1-12

Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated MyogenesisTernary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis

The M13 bacteriophage (M13 phage) has merged as a novel organic building block in biomedical applications. M13 phage is a ... The decoration of GO and RGD peptide was readily achieved by using RGD peptide-displaying M13 bacteriophage (RGD-M13 phage) and ... RGD peptide-displaying M13 bacteriophage/PLGA nanofibers as cell-adhesive matrices for smooth muscle cells. J Korean Phys Soc. ... Cell-adhesive RGD peptide-displaying M13 bacteriophage/PLGA nanofiber matrices for growth of fibroblasts. Biomater Res. 2014;18 ...
more infohttp://ntno.org/v02p0144.htm

Filamentous bacteriophage - WikipediaFilamentous bacteriophage - Wikipedia

M13 bacteriophage f1 phage fd phage Karlsson, F; Borrebaeck, CA; Nilsson, N; Malmborg-Hager, AC (April 2003). "The mechanism of ... A filamentous bacteriophage is a type of bacteriophage, or virus of bacteria, defined by its filament-like or rod-like shape. ... Russel, M; Model, P (August 1989). "Genetic analysis of the filamentous bacteriophage packaging signal and of the proteins that ...
more infohttps://en.wikipedia.org/wiki/Filamentous_bacteriophage

Category:Bacteriophages - Wikimedia CommonsCategory:Bacteriophages - Wikimedia Commons

Media in categorie "Bacteriophages". Deze categorie bevat de volgende 77 bestanden, van in totaal 77. ... ADVERTISEMENT; Antivirus and bacteriophages Wellcome L0032605.jpg 5.228 × 3.451; 7,23 MB. ... The arrangement of known genes of bacteriophage T12 after integration into host.png 917 × 456; 28 kB. ... Overgenomen van "https://commons.wikimedia.org/w/index.php?title=Category:Bacteriophages&oldid=310610186" ...
more infohttps://commons.wikimedia.org/wiki/Category:Bacteriophages?uselang=nl

Patent US6548242 - Process for the sterilization of biological compositions and the product thereby - Google PatentsPatent US6548242 - Process for the sterilization of biological compositions and the product thereby - Google Patents

1d depicts the virus kill results for bacteriophage M13.. FIG. 2 comprises two graphs depicting the results of the inactivation ... 3d depicts the virus kill results for bacteriophage M13.. FIG. 4 comprises two graphs depicting the results of the inactivation ... 1 comprises four graphs depicting the results of the inactivation by AMT and UVA of VSV and bacteriophage M13 in the presence ... 3 comprises four graphs depicting the results of the inactivation of VSV and bacteriophage M13 by AMT and UVA in the presence ...
more infohttp://www.google.com/patents/US6548242?dq=6446111

Browsing MIT Open Access Articles by TitleBrowsing MIT Open Access Articles by Title

M13 Bacteriophage Display Framework That Allows Sortase-Mediated Modification of Surface-Accessible Phage Proteins  Hess, ... We exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage. We show that pIII, ... We report a first method for using M13 bacteriophage as a multifunctional scaffold for optically imaging bacterial infections ... We demonstrate that M13 virus conjugated with hundreds of dye molecules (M13-Dye) can ... ...
more infohttps://dspace.mit.edu/handle/1721.1/49432/browse?rpp=20&sort_by=1&type=title&etal=-1&starts_with=M&order=ASC

David P Allison | ORNLDavid P Allison | ORNL

"Electron Microscopic Studies of M13 Bacteriophage DNA Replication," J. Virol. 24, 673-84. ... W. E. Masker, N. B. Kuemmerle, and D. P. Allison, (1978). "In vitro Packaging of Bacteriophage T7 DNA Synthesized in vitro," J ... M. E. Boling, J. K. Setlow, and D. P. Allison, (1972). "Bacteriophage of Haemophilus influenzae. I. Differences Between ... M. E. Boling, D. P. Allison, and J. K. Setlow, (1973). "Bacteriophage of Haemophilus influenzae III. Morphology// ...
more infohttps://www.ornl.gov/staff-profile/david-p-allison

Cloning Plasmids and DNAs | NEBCloning Plasmids and DNAs | NEB

Phage vectors derived from bacteriophage M13. *DNA, covalently closed circular. *13 Unique RE sites with β-gal gene ...
more infohttps://www.neb.com/tools-and-resources/selection-charts/cloning-plasmids-and-dnas

Rabbit Anti-Human Rad51 Polyclonal Antibody, Unconjugated from AbcamRabbit Anti-Human Rad51 Polyclonal Antibody, Unconjugated from Abcam

Peptide Display Cloning System facilitates the display ... of bacteriophage M13 as coat protein fusions, ... peptide and its ...
more infohttp://www.bio-medicine.org/biology-products/Rabbit-Anti-Human-Rad51-Polyclonal-Antibody--Unconjugated-from-Abcam-148-1/

DSpace@MIT: 
                Browsing Mechanical Engineering - Masters degree by Title[email protected]: Browsing Mechanical Engineering - Master's degree by Title

Engineering M13 bacteriophage platforms for cancer therapy applications  Tsedev, Uyanga (Massachusetts Institute of Technology ... Two novel schemes for engineering M13 bacteriophage for application in the diagnosis, imaging and treatment of human tumors are ... Firstly, by exploiting the uniquely malleable biology of the M13 filamentous phage, ... ...
more infohttp://dspace.mit.edu/handle/1721.1/7850/browse?order=ASC&rpp=20&sort_by=1&etal=-1&offset=1262&type=title

Single polypeptide chain binding molecules - Genex CorporationSingle polypeptide chain binding molecules - Genex Corporation

When hosts containing these plasmids are superinfected with bacteriophage M13 two types of progeny are produced, one containing ... The plasmids used contained an M13 bacteriophage origin of DNA replication. ... The lac control elements may be obtained from bacteriophage lambda plac5, which is infective for E. coli. The lac promoter- ... genetic construct for a single chain binding protein can be placed under the control of the leftward promoter of bacteriophage ...
more infohttp://www.freepatentsonline.com/4946778.html
  • Here we present a structural model for the capsid of intact M13 bacteriophage using Rosetta model building guided by structure restraints obtained from magic-angle spinning solid-state NMR experimental data. (rcsb.org)
  • This is an extension of the work initially reported in the journal Science in 2009 of genetically manipulating the M13 virus to support the synthesis and assembly of iron phosphate cathode materials for high-power lithium-ion batteries. (greencarcongress.com)
  • The two M13 viruses, genetically engineered for specificity (p8#9 virus) and versatility (E4/ E3 virus) served as a template for the synthesis of noble metal nanowires with diameters below 50 nm. (greencarcongress.com)
  • The inherent structural characteristic of the M13 virus enabled the synthesis of high aspect ratio nanowires. (greencarcongress.com)
  • With advantages of facile and environmentally benign synthesis, M13 biological platform proved itself as a useful toolkit for the study on the basic electrochemical property of materials. (greencarcongress.com)
  • Unlike some other types of phage, M13 does not carry genes for toxins. (alzforum.org)
  • defined growth conditions, conventional antibiotics, and antimicrobial peptides with some strain specificity, lytic bacteriophages, and the expression of antibiotic resistance genes, auxotrophic markers, or toxins under unique expression systems ( 1 ). (asm.org)
  • Here we would like to shed light on "Bacteriophage" the naturally available nanoparticle, which have been effectively utilized for its anti- microbial applications for different pathogens and in other fields of biomedicine. (alliedacademies.org)
  • We report the development of a novel genetically-tunable piezoelectric material based on biological nanoparticle M13 bacteriophage (phage) and its application in electronic and energy generating devices. (mrs.org)
  • This class includes strains fd, f1, M13 (these 3 very similar strains are members of the Ff group), If1 and IKe. (rcsb.org)
  • Our work includes the interaction studies between different bacteriophage strains with different PCa cell lines and to know how these particles modulate cancer cell behaviour in vitro ( Figure 1 ). (alliedacademies.org)
  • 3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII. (nih.gov)
  • An MIT team including Drs. Gerbrand Ceder and Angela Belcher has synthesized gold (Au) and silver (Ag) alloy nanowires as anode materials for Li-ion batteries using multiple clones of the M13 bacteriophage virus. (greencarcongress.com)
  • We describe molecular models of the class I filamentous bacteriophages. (rcsb.org)
  • The above stratagem should prove particularly advantageous in the preparation of assemblies of larger and more complex molecular architectures based on the M13 building block. (uwaterloo.ca)
  • CRAϕ is the first bacteriophage reported to require the molecular machinery involved in the uptake of environmental DNA for infection. (asm.org)
  • Bacteriophage Lambda Vector for Transducing a cDNA Clone Library into Mammalian Cells", Molecular and Cellular Biology 5:1136-1142 (1985). (patentgenius.com)
  • We need to investigate how the selected virus (likely one of the following: TMV, M13, CCMV, CPMV, BMV or TPMV) interacts with mammalian cells in vivo. (openwetware.org)
  • protective Immune Responses Induced by the Immunization of Mice with a Recombinant Bacteriophage Displaying a Epitope of the Human Respiratory Syncytial Virus" Virology 234: 118-122 (1997). (patentgenius.com)
  • 3. The immunogenic formulation hepatitis according to claim 1, wherein the bacteriophage has been engineered to express more than one hepatitis virus type A and/or type B polypeptide. (patentgenius.com)
  • A team from the University of California, Berkeley, is apparently making use of the virus, known as M12 bacteriophage, in order to replace toxic elements that are used to charge new and old cell phones. (sellcell.com)
  • This backbone is typically derived from a bacteriophage and is completely unique in sequence along its length. (nature.com)
  • The use of the s-form of the M13 bacteriophage significantly expands the templating capabilities of this viral platform and introduces the potential for further morphological control of a variety of inorganic material systems. (rsc.org)
  • In addition, the expression of Vascular Endothelial Growth Factor A (VEGF-A), VEGF Receptors 2 and 3, Matrix Metalloproteinase 9 (MMP9), and epithelial nitric oxide synthase (eNOS) transcripts were significantly upregulated due to the HUVECs culturing on M13 phage-RGD coated surface. (nature.com)
  • Furthermore, VEGF protein secretion, nitric oxide, and reactive oxygen species (ROS) production were significantly increased in cells cultured on M13 phage-RGD coated surface. (nature.com)
  • We would like to take this advantage to report our preliminary findings based upon the in vitro interaction studies from PCa cells (PC3) with bacteriophage T4 and M13. (alliedacademies.org)
  • Lytic bacteriophages often offer exquisite specificity ( 2 ). (asm.org)
  • However, individual bacteriophages must be isolated against each strain and would require additional screening to determine the degree of specificity. (asm.org)
  • M13 had to be engineered to have accessible insertion sites, making it limited in its flexibility in handling different sized inserts. (wikipedia.org)
  • In order to extend the versatility of M13 in this area, residue-specific unnatural amino acid incorporation was employed to successfully display azide functionalities on specific solvent-exposed positions of the pVIII major sheath protein of this bacteriophage. (uwaterloo.ca)
  • NeuroPhage's lead treatment is a natural variant of bacteriophage M13, depicted graphically and by electron microscopy. (alzforum.org)
  • The M13 phage, belonging to the family inoviridae, has a length of ∼1 μm and a diameter of ∼7 nm. (rcsb.org)
  • Each ShuA variant was analyzed for its ability to display on the bacteriophage surface, and functionally bind to hemoglobin. (rsc.org)
  • While different antimicrobial strategies, such as antibiotics, antimicrobial peptides, and lytic bacteriophages, offer partial solutions, what remains elusive is a generalized and programmable strategy that can distinguish between even closely related microorganisms and that allows for fine control over the composition of a microbial population. (asm.org)