Bacteriophage lambda
Lysogeny
Escherichia coli
Bacteriophage T4
Base Sequence
Bacteriophage T7
T-Phages
Viral Regulatory and Accessory Proteins
Recombination, Genetic
Mutation
Plasmids
Bacteriophage mu
Molecular Sequence Data
Attachment Sites, Microbiological
Genes
DNA Restriction Enzymes
Cloning, Molecular
Bacteriophage phi 6
Bacteriolysis
Bacteriophage phi X 174
Integrases
Viral Tail Proteins
Bacteriophage P2
Amino Acid Sequence
DNA-Directed RNA Polymerases
DNA, Recombinant
Bacteriophage M13
Operon
DNA Nucleotidyltransferases
Bacteriophage T3
Transcription, Genetic
Chromosome Mapping
Transduction, Genetic
Immunoglobulin lambda-Chains
Bacteriophage P1
Bacteriophage Typing
Integration Host Factors
Endodeoxyribonucleases
Salmonella Phages
Prophages
Siphoviridae
DNA Packaging
Genes, Regulator
Virus Replication
Nucleic Acid Conformation
RNA Phages
Viral Plaque Assay
Bacteriophage PRD1
Chromosomes, Bacterial
Nucleic Acid Hybridization
Pseudomonas Phages
Restriction Mapping
DNA
Adsorption
Bacillus Phages
Centrifugation, Density Gradient
Binding Sites
Genetics, Microbial
Genetic Complementation Test
Operator Regions, Genetic
Suppression, Genetic
DNA, Single-Stranded
Exonucleases
Rho Factor
Temperature
Microscopy, Electron
Repressor Proteins
DNA, Circular
Endonucleases
DNA-Binding Proteins
Levivirus
Gene Expression Regulation, Viral
Rec A Recombinases
Ultraviolet Rays
Polynucleotide Ligases
DNA, Superhelical
Receptors, Virus
Promoter Regions, Genetic
Templates, Genetic
Electrophoresis, Polyacrylamide Gel
Chloramphenicol
Nucleic Acid Heteroduplexes
Sequence Homology, Nucleic Acid
DNA Transposable Elements
Nucleic Acid Denaturation
Genetic Code
Inovirus
Protein Biosynthesis
Genetic Vectors
Electrophoresis, Agar Gel
Protein Binding
Exodeoxyribonuclease V
Species Specificity
Models, Molecular
Transcription Factors
Virus Assembly
DNA Helicases
Viral Structural Proteins
Sequence Analysis, DNA
Bacteriophage P22
Adenosine Triphosphatases
Extrachromosomal Inheritance
Protein Conformation
Viral Interference
Crosses, Genetic
Models, Genetic
Virus Activation
Immunoglobulin Light Chains
Blotting, Southern
Genetic Engineering
Porins
Maltose
ATP-Dependent Proteases
Mutagenesis
Substrate Specificity
RNA, Messenger
Phenotype
Phosphorus Isotopes
RNA, Bacterial
Cystoviridae
Galactokinase
Bacterial Outer Membrane Proteins
Oligonucleotides
Virus Integration
Exodeoxyribonucleases
Lac Operon
Cell-Free System
Bacteriophage Pf1
Caudovirales
R Factors
Mutagenesis, Site-Directed
Conjugation, Genetic
DNA-Directed DNA Polymerase
SOS Response (Genetics)
Peptide Elongation Factors
beta-Galactosidase
Gene Expression Regulation
Recombinant Fusion Proteins
Sequence Homology, Amino Acid
Protein Structure, Tertiary
Peptide Library
Sequence Alignment
Macromolecular Substances
Genotype
Immunoglobulin kappa-Chains
Crossing Over, Genetic
DNA Primase
DNA Repair
Transformation, Bacterial
Chromatography
Cryoelectron Microscopy
Drug Resistance, Microbial
Heat-Shock Proteins
Oligodeoxyribonucleotides
Gene Library
Galactosidases
Mitomycins
Structure-Activity Relationship
Open Reading Frames
Biological Therapy
Chromatography, DEAE-Cellulose
HSP40 Heat-Shock Proteins
Host Specificity
Codon
Culture Media
Genes, Lethal
Oligonucleotide Probes
Deoxyribonuclease I
Transplacement mutagenesis: a novel in situ mutagenesis system using phage-plasmid recombination. (1/2257)
Site-specific mutagenesis provides the ability to alter DNA with precision so that the function of any given gene can be more fully understood. Several methods of in vitro mutagenesis are time-consuming and imprecise, requiring the subcloning and sequencing of products. Here we describe a rapid, high fidelity method of in situ mutagenesis in bacteriophage lambda using transplacement. Using this method, mutations are transferred from oligonucleotides to target phages using a plasmid interface. A small (50 bp) homology region bearing a centred point mutation is generated from oligonucleotides and subcloned into a transplacement plasmid bearing positive and negative phage selectable markers. Following a positive/negative selection cycle of integrative recombination and excision, the point mutation is transferred precisely from plasmid to phage in a subset ( approximately 25-50%) of recombinants. As the fidelity of both oligonucleotide synthesis and phage-plasmid recombination is great, this approach is extremely reliable. Using transplacement, point mutations can be accurately deposited within large phage clones and we demonstrate the utility of this technique in the construction of gene targeting vectors in bacteriophages. (+info)Comparison of synonymous codon distribution patterns of bacteriophage and host genomes. (2/2257)
Synonymous codon usage patterns of bacteriophage and host genomes were compared. Two indexes, G + C base composition of a gene (fgc) and fraction of translationally optimal codons of the gene (fop), were used in the comparison. Synonymous codon usage data of all the coding sequences on a genome are represented as a cloud of points in the plane of fop vs. fgc. The Escherichia coli coding sequences appear to exhibit two phases, "rising" and "flat" phases. Genes that are essential for survival and are thought to be native are located in the flat phase, while foreign-type genes from prophages and transposons are found in the rising phase with a slope of nearly unity in the fgc vs. fop plot. Synonymous codon distribution patterns of genes from temperate phages P4, P2, N15 and lambda are similar to the pattern of E. coli rising phase genes. In contrast, genes from the virulent phage T7 or T4, for which a phage-encoded DNA polymerase is identified, fall in a linear curve with a slope of nearly zero in the fop vs. fgc plane. These results may suggest that the G + C contents for T7, T4 and E. coli flat phase genes are subject to the directional mutation pressure and are determined by the DNA polymerase used in the replication. There is significant variation in the fop values of the phage genes, suggesting an adjustment to gene expression level. Similar analyses of codon distribution patterns were carried out for Haemophilus influenzae, Bacillus subtilis, Mycobacterium tuberculosis and their phages with complete genomic sequences available. (+info)General method of analysis of kinetic equations for multistep reversible mechanisms in the single-exponential regime: application to kinetics of open complex formation between Esigma70 RNA polymerase and lambdaP(R) promoter DNA. (3/2257)
A novel analytical method based on the exact solution of equations of kinetics of unbranched first- and pseudofirst-order mechanisms is developed for application to the process of Esigma70 RNA polymerase (R)-lambdaPR promoter (P) open complex formation, which is described by the minimal three-step mechanism with two kinetically significant intermediates (I1, I2), [equation: see text], where the final product is an open complex RPo. The kinetics of reversible and irreversible association (pseudofirst order, [R] >> [P]) to form long-lived complexes (RPo and I2) and the kinetics of dissociation of long-lived complexes both exhibit single exponential behavior. In this situation, the analytical method provides explicit expressions relating observed rate constants to the microscopic rate constants of mechanism steps without use of rapid equilibrium or steady-state approximations, and thereby provides a basis for interpreting the composite rate constants of association (ka), isomerization (ki), and dissociation (kd) obtained from experiment for this or any other sequential mechanism of any number of steps. In subsequent papers, we apply this formalism to analyze kinetic data obtained in the reversible and irreversible binding regimes of Esigma70 RNA polymerase (R)-lambdaP(R) promoter (P) open complex formation. (+info)Single-polymer dynamics in steady shear flow. (4/2257)
The conformational dynamics of individual, flexible polymers in steady shear flow were directly observed by the use of video fluorescence microscopy. The probability distribution for the molecular extension was determined as a function of shear rate, gamma;, for two different polymer relaxation times, tau. In contrast to the behavior in pure elongational flow, the average polymer extension in shear flow does not display a sharp coil-stretch transition. Large, aperiodic temporal fluctuations were observed, consistent with end-over-end tumbling of the molecule. The rate of these fluctuations (relative to the relaxation rate) increased as the Weissenberg number, gamma;tau, was increased. (+info)Cloning of mnuA, a membrane nuclease gene of Mycoplasma pulmonis, and analysis of its expression in Escherichia coli. (5/2257)
Membrane nucleases of mycoplasmas are believed to play important roles in growth and pathogenesis, although no clear evidence for their importance has yet been obtained. As a first step in defining the function of this unusual membrane activity, studies were undertaken to clone and analyze one of the membrane nuclease genes from Mycoplasma pulmonis. A novel screening strategy was used to identify a recombinant lambda phage expressing nuclease activity, and its cloned fragment was analyzed. Transposon mutagenesis was used to identify an open reading frame of 1,410 bp, which coded for nuclease activity in Escherichia coli. This gene coded for a 470-amino-acid polypeptide of 53,739 Da and was designated mnuA (for "membrane nuclease"). The MnuA protein contained a prolipoprotein signal peptidase II recognition sequence along with an extensive hydrophobic region near the amino terminus, suggesting that the protein may be lipid modified or that it is anchored in the membrane by this membrane-spanning region. Antisera raised against two MnuA peptide sequences identified an M. pulmonis membrane protein of approximately 42 kDa by immunoblotting, which corresponded to a trypsin-sensitive nucleolytic band of the same size. Maxicell experiments with E. coli confirmed that mnuA coded for a nuclease of unknown specificity. Hybridization studies showed that mnuA sequences are found in few Mycoplasma species, suggesting that mycoplasma membrane nucleases display significant sequence variation within the genus Mycoplasma. (+info)Construction and analysis of hybrid Escherichia coli-Bacillus subtilis dnaK genes. (6/2257)
The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify the regions responsible for this finding. It turned out that the B. subtilis DnaK protein needed approximately normal amounts of the cochaperone DnaJ to be functional in E. coli. The ATPase domain and the substrate-binding domain form a species-specific functional unit, while the C-terminal domains, although less conserved, are exchangeable. Deletion of the C-terminal domain in E. coli DnaK affected neither complementation of growth at high temperatures nor propagation of phage lambda but abolished degradation of sigma32. (+info)X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture. (7/2257)
Phage T4 endonuclease VII (Endo VII), the first enzyme shown to resolve Holliday junctions, recognizes a broad spectrum of DNA substrates ranging from branched DNAs to single base mismatches. We have determined the crystal structures of the Ca2+-bound wild-type and the inactive N62D mutant enzymes at 2.4 and 2.1 A, respectively. The Endo VII monomers form an elongated, highly intertwined molecular dimer exhibiting extreme domain swapping. The major dimerization elements are two pairs of antiparallel helices forming a novel 'four-helix cross' motif. The unique monomer fold, almost completely lacking beta-sheet structure and containing a zinc ion tetrahedrally coordinated to four cysteines, does not resemble any of the known junction-resolving enzymes, including the Escherichia coli RuvC and lambda integrase-type recombinases. The S-shaped dimer has two 'binding bays' separated by approximately 25 A which are lined by positively charged residues and contain near their base residues known to be essential for activity. These include Asp40 and Asn62, which function as ligands for the bound calcium ions. A pronounced bipolar charge distribution suggests that branched DNA substrates bind to the positively charged face with the scissile phosphates located near the divalent cations. A model for the complex with a four-way DNA junction is presented. (+info)A RAPID algorithm for sequence database comparisons: application to the identification of vector contamination in the EMBL databases. (8/2257)
MOTIVATION: Word-matching algorithms such as BLAST are routinely used for sequence comparison. These algorithms typically use areas of matching words to seed alignments which are then used to assess the degree of sequence similarity. In this paper, we show that by formally separating the word-matching and sequence-alignment process, and using information about word frequencies to generate alignments and similarity scores, we can create a new sequence-comparison algorithm which is both fast and sensitive. The formal split between word searching and alignment allows users to select an appropriate alignment method without affecting the underlying similarity search. The algorithm has been used to develop software for identifying entries in DNA sequence databases which are contaminated with vector sequence. RESULTS: We present three algorithms, RAPID, PHAT and SPLAT, which together allow vector contaminations to be found and assessed extremely rapidly. RAPID is a word search algorithm which uses probabilities to modify the significance attached to different words; PHAT and SPLAT are alignment algorithms. An initial implementation has been shown to be approximately an order of magnitude faster than BLAST. The formal split between word searching and alignment not only offers considerable gains in performance, but also allows alignment generation to be viewed as a user interface problem, allowing the most useful output method to be selected without affecting the underlying similarity search. Receiver Operator Characteristic (ROC) analysis of an artificial test set allows the optimal score threshold for identifying vector contamination to be determined. ROC curves were also used to determine the optimum word size (nine) for finding vector contamination. An analysis of the entire expressed sequence tag (EST) subset of EMBL found a contamination rate of 0.27%. A more detailed analysis of the 50 000 ESTs in est10.dat (an EST subset of EMBL) finds an error rate of 0.86%, principally due to two large-scale projects. AVAILABILITY: A Web page for the software exists at http://bioinf.man.ac.uk/rapid, or it can be downloaded from ftp://ftp.bioinf.man.ac.uk/RAPID CONTACT: [email protected] (+info)Some common effects of chromosomal deletions include:
1. Genetic disorders: Chromosomal deletions can lead to a variety of genetic disorders, such as Down syndrome, which is caused by a deletion of a portion of chromosome 21. Other examples include Prader-Willi syndrome (deletion of chromosome 15), and Williams syndrome (deletion of chromosome 7).
2. Birth defects: Chromosomal deletions can increase the risk of birth defects, such as heart defects, cleft palate, and limb abnormalities.
3. Developmental delays: Children with chromosomal deletions may experience developmental delays, learning disabilities, and intellectual disability.
4. Increased cancer risk: Some chromosomal deletions can increase the risk of developing certain types of cancer, such as chronic myelogenous leukemia (CML) and breast cancer.
5. Reproductive problems: Chromosomal deletions can lead to reproductive problems, such as infertility or recurrent miscarriage.
Chromosomal deletions can be diagnosed through a variety of techniques, including karyotyping (examination of the chromosomes), fluorescence in situ hybridization (FISH), and microarray analysis. Treatment options for chromosomal deletions depend on the specific effects of the deletion and may include medication, surgery, or other forms of therapy.
Harvey Bialy
Jean Weigle
Esther Lederberg
Helios Murialdo
Sankar Adhya
Lambda phage
Exodeoxyribonuclease (lambda-induced)
Genome
Daisy Roulland-Dussoix
Allan M. Campbell
Asilomar Conference on Recombinant DNA
Interactome
DNA binding site
Genetically modified virus
Richard Lenski
Zdeněk Neubauer
Shiga toxin
Inclusion bodies
Lambda holin family
Prohead
CII protein
Phage monographs
Bacteriophage
PstI
Maltoporin
GrpE
RNA thermometer
Marlene Belfort
Helix-turn-helix
Biomolecular engineering
YqaJ protein domain
Laboratory experiments of speciation
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing
Marc Zabeau
Vector (molecular biology)
Okazaki fragments
Glycoside hydrolase family 24
BlyA holin family
NAS Award in Molecular Biology
Walter Fiers
Leslie Barnett
Restriction enzyme
Lysin
Homeobox
Cosmid
Virus nanotechnology
Genomic library
Bill Earnshaw
CEACAM5
Bernard Ogilvie Dodge
Gaussian network model
Zinc finger protein 226
Cloning vector pi-VX used for screening bacteriophage lambda gene libr - Nucleotide - NCBI
The nut site of bacteriophage lambda is made of RNA and is bound by transcription antitermination factors on the surface of RNA...
DNA Looping fine-tunes the extreme stability of the prophage state of Bacteriophage Lambda in a lysogen | NIH Research Festival
Isolation and characterization of specialized lambda transducing bacteriophage carrying the metBJF methionine gene cluster<...
Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning - Wikidata
Phage-like particle vaccines are highly immunogenic and protect against pathogenic coronavirus infection and disease | npj...
Frontiers | Phages in the Human Body
Publication Detail
Clonal Spread of Streptococcus pyogenes emm44 among Homeless Persons, Rennes, France - Volume 17, Number 2-February 2011 -...
Pastan, Ira 2008 A - Office of NIH History and Stetten Museum
DDC | Free Full-Text | Optimizing Protein Production in Therapeutic Phages against a Bacterial Pathogen, Mycobacterium abscessus
Biomarkers Search
MeSH Browser
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Scott Meyers: Articles and Interviews
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DeCS
Phage lambda6
- Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. (wikidata.org)
- Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. (wikidata.org)
- The construction in vitro of transducing derivatives of phage lambda. (wikidata.org)
- Bacteriophage (phage) Lambda (λ) has played a key historic role in driving our current understanding of molecular genetics. (uwaterloo.ca)
- In addition, we have found that an independent insertion in a transducing phage, lambda 13 dargB2, is IS5. (archive.org)
- Kaiser had done much work on Phage lambda, a lysogenic virus that infects E. coli bacteria. (nih.gov)
Phages2
- 3. Mammalian cell transduction and internalization properties of lambda phages displaying the full-length adenoviral penton base or its central domain. (nih.gov)
- Over his 44-year career at the Texas A&M College of Medicine and College of Agriculture and Life Sciences(external link), Young has made broad advances in the understanding of bacteria-infecting viruses called bacteriophages, or phages. (nih.gov)
Viruses5
- Bacteriophages, viruses that infect bacteria, have re-emerged as powerful regulators of bacterial populations in natural ecosystems. (frontiersin.org)
- in general, bacteriophages have deserved less interest in comparison to their bacterial hosts or to animal viruses. (frontiersin.org)
- A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE . (nih.gov)
- Bacteriophages, viruses that specifically infect bacteria, may be considered as structurally simplistic protein-based vehicles for ferrying nucleic acid cargo. (uwaterloo.ca)
- In a 1965 presentation, Kaiser suggested that the mechanisms at work in the bacteriophage lambda might find analogues in mammalian tumor viruses such as simian virus 40 (SV40) and polyoma which cause tumors in monkeys and mice, respectively. (nih.gov)
Capsid1
- 19. Display libraries on bacteriophage lambda capsid. (nih.gov)
Coli3
- Background: One of the best understood systems in genetic regulatory biology is the life-cycles of Bacteriophage Lambda in E. coli. (nih.gov)
- en] The molecular bases of ascorbic acid effects in SOS functions of E.Coli are studied. (iaea.org)
- Bacteriophage lambda interacts with E. coli proteins. (string-db.org)
Characterization1
- Johnson, JR , Greene, RC & Krueger, JH 1977, ' Isolation and characterization of specialized lambda transducing bacteriophage carrying the metBJF methionine gene cluster ', Journal of Bacteriology , vol. 131, no. 3, pp. 795-800. (umn.edu)
Eukaryotic2
- Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. (wikidata.org)
- 13. Chemical coupling as a potent strategy for preparation of targeted bacteriophage-derived gene nanocarriers into eukaryotic cells. (nih.gov)
Bacteria2
- The DNA complement of bacteriophage lambda is infective if certain conditions of bacteria and "helper" phage are satisfied (Kaiser & Hogness, 1960). (caltech.edu)
- However, after infection of sensitive bacteria by lambda phage, most of the injected DNA appears to lose its infectivity, at least as measured under these conditions. (caltech.edu)
Lysis2
- He was a National Institutes of Health, NIH, Postdoctoral Fellow at Harvard Medical School, where he discovered a bacteriophage lambda gene involved in lysis. (nih.gov)
- Bacteriophage Rz lysis protein [Interproscan]. (ntu.edu.sg)
Genetic1
- Examples of first attempts to understand these phenomena can be found at all levels of biological organization, including the modeling of the genetic circuitry of bacteriophage lambda regulation, the modeling of the yeast cell division cycle, and the quantitation of cellular processes such as metabolic flux and response to stress. (nih.gov)
Vectors3
- 4. In vivo gene delivery and expression by bacteriophage lambda vectors. (nih.gov)
- 16. Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice. (nih.gov)
- 20. Polycos vectors: a system for packaging filamentous phage and phagemid vectors using lambda phage packaging extracts. (nih.gov)
Antigens2
- We developed a "designer nanoparticle" platform using phage-like particles (PLPs) derived from bacteriophage lambda for a multivalent display of antigens in rigorously defined ratios. (nature.com)
- Researchers at National Cancer Institute (NCI) developed an engineered bacteriophage lambda () vector for displaying antigens as a vaccine in the treatment of cancer and infectious diseases. (nih.gov)
Protein6
- The boxA and boxB components of the lambda nut site are important for transcriptional antitermination by the phage N protein. (nih.gov)
- A protein-RNA interaction network facilitates the template-independent cooperative assembly on RNA polymerase of a stable antitermination complex containing the lambda N protein. (nih.gov)
- 15. Biotin-tagged cDNA expression libraries displayed on lambda phage: a new tool for the selection of natural protein ligands. (nih.gov)
- incubating the concatamer with a recombination protein to generate circular nucleic acids, wherein the recombination protein is chosen from a Cre recombinase, a bacteriophage lambda integrase, a yeast Flp recombinase, or a bacterial XerCD recombinase. (epo.org)
- Bacteriophage CII protein [Interproscan]. (ntu.edu.sg)
- In this technology, a nucleic acid sequence encoding a fusion protein linked to a heterologous antigen is inserted into a native gene D locus adjacent to gene E in the bacteriophage lambda genome. (nih.gov)
Gene delivery1
- 5. A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery. (nih.gov)
Extracts1
- We also find that foci form on both sperm chromatin and bacteriophage lambda DNA incubated in extracts depleted of cdk2 kinase. (rupress.org)
CDNA2
Mammalian5
- 1. Proteasome inhibitors enhance bacteriophage lambda (lambda) mediated gene transfer in mammalian cells. (nih.gov)
- 2. Fc receptor-mediated, antibody-dependent enhancement of bacteriophage lambda-mediated gene transfer in mammalian cells. (nih.gov)
- 6. Mammalian cell binding and transfection mediated by surface-modified bacteriophage lambda. (nih.gov)
- 7. Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. (nih.gov)
- 9. Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. (nih.gov)
SIPHOVIRIDAE1
- Fago temperado inducible y especie tipo de los virus de género similar a lambda, de la familia SIPHOVIRIDAE. (bvsalud.org)
Proteins3
Production1
- 17. The production of generalized transducing phage by bacteriophage lambda. (nih.gov)
System1
- The P R promoter system used in the empirical measurements consists of a strong lambda phage P R promoter (RNAP-binding site) and two CI operator sites (transcription factor binding sites O R1 and O R2 ), which control the expression of a venus-yfp reporter gene. (elifesciences.org)
PHAGE LAMBDA2
- Kaiser had done much work on Phage lambda, a lysogenic virus that infects E. coli bacteria. (nih.gov)
- In contrast to phage lambda, mEp021 virus particle production was partially restored (>1/3 relative to wild type) when nus mutants (nusA1, nusB5, nusC60, and nusE71) were infected with mEp021 and Gp17 was overexpressed. (bvsalud.org)
Gene7
- Sequence specificity of mutagenesis in the cI gene of bacteriophage lambda. (nih.gov)
- We have developed a system for the analysis of chemically induced base sequence alterations in the cI repressor gene of bacteriophage lambda using DNA sequencing techniques. (nih.gov)
- 21. Binding properties, cell delivery, and gene transfer of adenoviral penton base displaying bacteriophage. (nih.gov)
- 22. Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. (nih.gov)
- 23. In vivo gene delivery and expression by bacteriophage lambda vectors. (nih.gov)
- He was a National Institutes of Health, NIH, Postdoctoral Fellow at Harvard Medical School, where he discovered a bacteriophage lambda gene involved in lysis. (nih.gov)
- In this technology, a nucleic acid sequence encoding a fusion protein linked to a heterologous antigen is inserted into a native gene D locus adjacent to gene E in the bacteriophage lambda genome. (nih.gov)