A tentative species in the genus lambda-like viruses, family SIPHOVIRIDAE.
Viruses whose hosts are bacterial cells.
A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.
Viruses whose host is Escherichia coli.
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.
Proteins that form the CAPSID of VIRUSES.
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.
Proteins found in any species of virus.
A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Viruses whose nucleic acid is DNA.
Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7.
A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.
A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.
Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
The functional hereditary units of VIRUSES.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Viruses whose host is Staphylococcus.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
A family of bacteriophages which are characterized by short, non-contractile tails.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Viruses whose host is Streptococcus.
Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The process by which a DNA molecule is duplicated.
Genomes of temperate BACTERIOPHAGES integrated into the DNA of their bacterial host cell. The prophages can be duplicated for many cell generations until some stimulus induces its activation and virulence.
A genus of filamentous bacteriophages of the family INOVIRIDAE. Organisms of this genus infect enterobacteria, PSEUDOMONAS; VIBRIO; and XANTHOMONAS.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
A subdiscipline of genetics which deals with the genetic mechanisms and processes of microorganisms.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Specific loci on both the bacterial DNA (attB) and the phage DNA (attP) which delineate the sites where recombination takes place between them, as the phage DNA becomes integrated (inserted) into the BACTERIAL DNA during LYSOGENY.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
A species of gram-positive bacteria that is a common soil and water saprophyte.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A family of trypsin-like SERINE ENDOPEPTIDASES that are expressed in a variety of cell types including human prostate epithelial cells. They are formed from tissue prokallikrein by action with TRYPSIN. They are highly similar to PROSTATE-SPECIFIC ANTIGEN.

Activity of coliphage HK022 excisionase (Xis) in the absence of DNA binding. (1/10)

A mutated excisionase (Xis) protein of coliphage HK022 whose single Cys residue was replaced by Ser does not bind to its two tandem binding sites (X1, X2) on the P arm of attR. Despite its DNA-binding inability the protein showed 30% excision activity of the wild type Xis both in vitro and in vivo. This partial activity is attributed to the interaction of Xis with integrase that is retained in the mutant protein. This protein-protein interaction occurs in the absence of DNA binding.  (+info)

Suppression of factor-dependent transcription termination by antiterminator RNA. (2/10)

Nascent transcripts of the phage HK022 put sites modify the transcription elongation complex so that it terminates less efficiently at intrinsic transcription terminators and accelerates through pause sites. We show here that the modification also suppresses termination in vivo at two factor-dependent terminators, one that depends on the bacterial Rho protein and a second that depends on the HK022-encoded Nun protein. Suppression was efficient when the termination factors were present at physiological levels, but an increase in the intracellular concentration of Nun increased termination both in the presence and absence of put. put-mediated antitermination thus shows no apparent terminator specificity, suggesting that put inhibits a step that is common to termination at the different types of terminator.  (+info)

Solution structure and stability of the full-length excisionase from bacteriophage HK022. (3/10)

Heteronuclear high-resolution NMR spectroscopy was employed to determine the solution structure of the excisionase protein (Xis) from the lambda-like bacteriophage HK022 and to study its sequence-specific DNA interaction. As wild-type Xis was previously characterized as a generally unstable protein, a biologically active HK022 Xis mutant with a single amino acid substitution Cys28-->Ser was used in this work. This substitution has been shown to diminish the irreversibility of Xis denaturation and subsequent degradation, but does not affect the structural or thermodynamic properties of the protein, as evidenced by NMR and differential scanning calorimetry. The solution structure of HK022 Xis forms a compact, highly ordered protein core with two well-defined alpha-helices (residues 5-11 and 18-27) and five beta-strands (residues 2-4, 30-31, 35-36, 41-44 and 48-49). These data correlate well with 1H2O-2H2O exchange experiments and imply a different organization of the HK022 Xis secondary structure elements in comparison with the previously determined structure of the bacteriophage lambda excisionase. Superposition of both Xis structures indicates a better correspondence of the full-length HK022 Xis to the typical 'winged-helix' DNA-binding motif, as found, for example, in the DNA-binding domain of the Mu-phage repressor. Residues 51-72, which were not resolved in the lambda Xis, do not show any regular structure in HK022 Xis and thus appear to be completely disordered in solution. The resonance assignments have shown, however, that an unusual connectivity exists between residues Asn66 and Gly67 owing to asparagine-isoaspartyl isomerization. Such an isomerization has been previously observed and characterized only in eukaryotic proteins.  (+info)

Transcription termination by phage HK022 Nun is facilitated by COOH-terminal lysine residues. (4/10)

The 109-amino acid Nun protein of prophage HK022 excludes superinfecting bacteriophage lambda by blocking transcription elongation on the lambda chromosome. Multiple interactions between Nun and the transcription elongation complex are involved in this reaction. The Nun NH(2)-terminal arginine-rich motif binds BOXB sequence in nascent lambda transcripts, whereas the COOH terminus binds RNA polymerase and contacts DNA template. Nun Trp(108) is required for interaction with DNA and transcription arrest. We analyzed the role of the adjacent Lys(106) and Lys(107) residues in the Nun reaction. Substitution of the lysine residues with arginine (K106R/K107R) had no effect on transcription arrest in vitro or in vivo. Nun K106A/K107A was partially active, whereas Nun K106D/K107D was defective in vitro and failed to exclude lambda. All mutants bound RNA polymerase and BOXB. In contrast to Nun K106R/K107R and K106A/K107A, Nun K106D/K107D did not cross-link DNA template. These results suggest that transcription arrest is facilitated by electrostatic interactions between positively charged Nun residues Lys(106) and Lys(107) and negatively charged DNA phosphate groups. These may assist intercalation of Trp(108) into template.  (+info)

Phosphorylation of the integrase protein of coliphage HK022. (5/10)

 (+info)

In vitro reconstitution and substrate specificity of a lantibiotic protease. (6/10)

 (+info)

Overexpression of phage HK022 Nun protein is toxic for Escherichia coli. (7/10)

 (+info)

Inhibition of a transcriptional pause by RNA anchoring to RNA polymerase. (8/10)

 (+info)

The crystal structure of the double-stranded DNA bacteriophage HK97 mature empty capsid was determined at 3.6 angstrom resolution. The 660 angstrom diameter icosahedral particle contains 420 subunits with a new fold. The final capsid maturation step is an autocatalytic reaction that creates 420 isopeptide bonds between proteins. Each subunit is joined to two of its neighbors by ligation of the side-chain lysine 169 to asparagine 356. This generates 12 pentameric and 60 hexameric rings of covalently joined subunits that loop through each other, creating protein chainmail: topologically linked protein catenanes arranged with icosahedral symmetry. Catenanes have not been previously observed in proteins and provide a stabilization mechanism for the very thin HK97 capsid. ...
Typically, of course, a nun is not a mother. The linguistic background of the word nun, however, is fertile when it comes to children. Its Late Latin ancestor
Swap large genomic regions for the generation of humanized models, or mutant models for comparative studies of several mutations.
Smaller in length and height than the standard HK45, the HK45 Compact (HK45C) uses a slim-line grip profile, otherwise is nearly identical in features to the full-size HK45. Like the HK45, the
聚财棋牌推广二维码 ♛【 1331.hk 】♛ Aa是亚洲最大自主品牌游戏平台,百万大奖等您来拿,聚财棋牌推广二维码秒送46就可以领取各种活动优惠,无需申请自动,提供ag真人娱乐,pt电子游戏,mg电子游戏,沙巴体育游戏,官网提供7*24小时客服服务,客户至上。
摤白单彦,吇泚吒宔於挋実「 1331.hk 「扎朶片田脔実戸竵三载,杁娄幱台324導旴眣亻圧纼3佢皈罗三抑泰馏逍
捺抧卶旴毐切罗宔於挋実「 1331.hk 「扎朶片田脔実戸竵三载,杁娄幱台324導旴眣亻圧纼3佢皈罗三抑泰馏逍
亄仧捎鲂帤冯冺舵宔於挋実「 1331.hk 「扎朶片田脔実戸竵三载,杁娄幱台324導旴眣亻圧纼3佢皈罗三抑泰馏逍
Tämä sähköpostiosoite on suojattu spamboteilta. Tarvitset JavaScript-tuen nähdäksesi sen.. Henkilökohtaiset sähköpostiosoitteet: ...
Tämä sähköpostiosoite on suojattu spamboteilta. Tarvitset JavaScript-tuen nähdäksesi sen.. Henkilökohtaiset sähköpostiosoitteet: ...
內臟脂肪 是甚麼?簡單來說就是集中在腹控內的脂肪,由外觀來說若你就算四肢不算胖,但擁有一個減不掉的「肥肚腩」那就有可能是內臟脂肪在作怪,而常常「久坐」的上班族就要小心內臟脂肪找上你!累積過多的內臟脂肪,還可能引起心臟疾病、糖尿病等等,是一個很大的健康問題呢!
- 搜尋世界各地拍賣行的未來拍賣會。Barnebys集中所有拍賣於一地。古董、傢俱、藝術和設計等等。
準誠分子診斷中心有限公司是一所分子基因測試公司,專門為客戶提供一系列的基因測試,親子鑒定,基因診斷,血緣關係診斷服務,我們的服務包括各種病原體的分子測試、親屬關係DNA驗證及遺傳性癌症風險檢測等,務求提供最高質素的檢測服務。
The HK45 was developed as a product improvement of the highly regarded USP45, first released in the 1995. The Caliber .45 ACP HK45 includes several user-inspired enhancements including changeable
Kinder, die durch einen Kaiserschnitt geboren werden, haben ein höheres Risiko an Asthma zu erkranken, als Kinder, die vaginal entbunden werden. Eine Studie zeigt nun, dass dies jedoch nur dann der Fall ist, wenn sich die Darmflora nicht bis zum ersten Geburtstag normal entwickelt.
說時遲那時快,開始倒數婚期11個月了~其實很多大Item都已經一早有定案,餘下的都只是一些濕碎的項目有待完成,但是大Item之中的「婚紗禮服」我們還遲 ... ,Beautylife HK
ALS-tutkimuksen tuki ry Y-tunnus: 2692744-5 Yhteystiedot: Tämä sähköpostiosoite on suojattu spamboteilta. Tarvitset JavaScript-tuen nähdäksesi sen. do...
上年小V在旅遊雜誌上見到介紹台灣秘景,其中一站是位於彰化,有全台灣唯一的海牛採蚵活動,雜誌圖片再配上迷人的芳苑日落,令彰化芳苑成為小V旅行的口袋名單之一。很幸運上年成功搶到HK$22機票到台中,使我這次可以有藉口到台中附近的彰化走走。. 談到彰化旅行,最煩就是交通的問題… 除了彰化市,基本上我覺得整個彰化的交通系統不太完善。說是要到芳苑去,一個位於彰化西邊沿海又很偏僻的地方,台灣好行的班次由平日每天2、3班至假日每天6班也有。再者,海牛採蚵活動在早上8點開始嚕,根本沒有那麼早的公車。不用煩… 不用煩… 最後,小V決定包車遊彰化一天。. 我跟大灰熊就是由彰化玩到台中去,玩一天,晚上宿台中。主要的行程都在彰化,但當天司機大哥說車子會順路經過台中彩虹眷村,所以又多了一個景點。呵!. ...
Frequency of bacteriuria and distribution of blood pressure, by age, were determined in 2,882 white and 422 negro nuns and 2,302 white and 396 negro control women working in similar occupations. Bacteriuria was 12.8, 2.8, and 1.4 times more frequent among white control women than among white nuns at ages 15 to 34, 35 to 54, and 55 or more years, respectively. It was 14.2 and 5.3 times higher for negro control women than for nuns aged 15 to 34 and 35 to 54 years. These differences were highly significant statistically. The prevalence of bacteriuria among nuns aged 15 to ...
The official movie page for watch The Nun online. Download The Nun or Learn more about cast, characters, plot, release date, & more!
Things are going better since my earlier post but I just read over what I had written and found the above simile. Thats terrible. Do you understand the self-doubt thing now? Yes? Good ...
2F-Ketamin ist eins der neuesten welches synthetisiert wurde nun auf dem Markt angeboten wird. 2F-Ketamin ab129. Entdeckte weitere interessante Chemik
Jessica Rabbit here a.k.a. tinkygreen a.k.a nun a.k.a. kat. People call me many different things, call me what you will-whats in a name anyways. So, here i am and there you are, lets disscuss...whatever...im intelligent, ask me anything.
仾牝牝纼跭梻浉「 VIP白彐連遐: 1331.hk 「亚洳固陂扎朶片実戸竵三载,佌亏菱徇寀驭尻拈,亚渳雇囤迋萞,戌仨丶甯戸揓侙曳奴皈安公俜際3曾佭渳戌幱台臲劘亏扙連亚洳曾佭皈渳戌幱台
沮单棆牒渳戌公叻罗三泰再「水乁罗圽:1331.hk「单趉哃败铵連俜誊幱台3乌幱台泰再斱亻且亥VIP,枃連扎朶片泰再実戸竵竉卶亥叚,赾夛奴礹驭三杢挀,夦颚旨快,24導旴玫圧実朔
丫禇罗彦祰台俜吒纼跭梻浉「 VIP白彐連遐: 1331.hk 「亚洳固陂扎朶片実戸竵三载,佌亏菱徇寀驭尻拈,亚渳雇囤迋萞,戌仨丶甯戸揓侙曳奴皈安公俜際3曾佭渳戌幱台臲劘亏扙連亚洳曾佭皈渳戌幱台
抧厌牝牝8883纼跭梻浉「 VIP白彐連遐: 1331.hk 「亚洳固陂扎朶片実戸竵三载,佌亏菱徇寀驭尻拈,亚渳雇囤迋萞,戌仨丶甯戸揓侙曳奴皈安公俜際3曾佭渳戌幱台臲劘亏扙連亚洳曾佭皈渳戌幱台
乁赧眣亻单彦纼跭梻浉「 VIP白彐連遐: 1331.hk 「亚洳固陂扎朶片実戸竵三载,佌亏菱徇寀驭尻拈,亚渳雇囤迋萞,戌仨丶甯戸揓侙曳奴皈安公俜際3曾佭渳戌幱台臲劘亏扙連亚洳曾佭皈渳戌幱台
Today I am feeling vindicated. And that vindication comes from a very strange source - the US attorney general, William P. Barr. Or, more specifically, his letter to the US Senate released to the public today. His letter reports the principal conclusions of Special Counsel, Robert S. Mueller on what has been dubbed Russiagate. That…
Concord New Hampshire Reason #197 Adam Paul Green (www.IMAdamGreen.com) Outgrew a Chocolate MLM Company and Joined the Rain-International Team (www.myrainlife.com/g3) was the Black Cumin Seed Purity
The use of synthetic biological systems in research, healthcare, and manufacturing often requires autonomous history-dependent behavior and therefore some form of engineered biological memory. For example, the study or reprogramming of aging, cancer, or development would benefit from genetically encoded counters capable of recording up to several hundred cell division or differentiation events. Although genetic material itself provides a natural data storage medium, tools that allow researchers to reliably and reversibly write information to DNA in vivo are lacking. Here, we demonstrate a rewriteable recombinase addressable data (RAD) module that reliably stores digital information within a chromosome. RAD modules use serine integrase and excisionase functions adapted from bacteriophage to invert and restore specific DNA sequences. Our core RAD memory element is capable of passive information storage in the absence of heterologous gene expression for over 100 cell divisions and can be switched ...
There was never any doubt that Cersei would find a way to get back at the Faith for what they put her through, and in the Season 6 finale she followed through on that promise. Cersei gets revenge on the shame nun on Game of Thrones, and its as…
Two doctors have suggested that the Catholic nuns must be given contraceptive pills to cut the extra cancer risk linked with chastity.
DHARAMSHALA: Yeshi Khando, a Tibetan nun self-immolated on 8 April whose state remained unknown has now been confirmed of death according to a reliable source. Yeshi Khando, also known as Yeyang, was a 47-year-old daughter of father Sherab and mother Sonam Dolma, who hailed from Drangsur village in
blockquote class=wp-embedded-content,,a href=http://andesexpedition.co.uk/ab/index.php/2016/11/21/acclimatisation-nuns-punctures-and-rescues/,Acclimatisation, Nuns, punctures and rescues!,/a,,/blockquote, ,script type=text/javascript, ,!--//--,,![CDATA[//,,!-- !function(a,b){use strict;function c(){if(!e){e=!0;var a,c,d,f,g=-1!==navigator.appVersion.indexOf(MSIE 10),h=!!navigator.userAgent.match(/Trident.*rv:11\./),i=b.querySelectorAll(iframe.wp-embedded-content);for(c=0;c,i.length;c++)if(d=i[c],!d.getAttribute(data-secret)){if(f=Math.random().toString(36).substr(2,10),d.src+=#?secret=+f,d.setAttribute(data-secret,f),g,,h)a=d.cloneNode(!0),a.removeAttribute(security),d.parentNode.replaceChild(a,d)}else;}}var d=!1,e=!1;if(b.querySelector)if(a.addEventListener)d=!0;if(a.wp=a.wp,,{},!a.wp.receiveEmbedMessage)if(a.wp.receiveEmbedMessage=function(c){var d=c.data;if(d.secret,,d.message,,d.value)if(!/[^a-zA-Z0-9]/.test(d.secret)){var ...
Due to member requests, Ive added an HK forum. The rules here are the same as any other UZI Talk forum - keep it legal and dont attack any other members. In relation to the HK family of weapons, please keep these things in mind: - HK snobbery is not welcome here. Its just a gun. - HK Clones are a welcome part of the HK discussion. - Feel free to talk about the positives and negatives of any HK related items - just dont make it personal.
Due to member requests, Ive added an HK forum. The rules here are the same as any other UZI Talk forum - keep it legal and dont attack any other members. In relation to the HK family of weapons, please keep these things in mind: - HK snobbery is not welcome here. Its just a gun. - HK Clones are a welcome part of the HK discussion. - Feel free to talk about the positives and negatives of any HK related items - just dont make it personal.
Furthermore, Donohue criticized Davids comments during his Wednesday appearance on Conan. Donohue said, He said to Conan OBrien that dressing as a nun in the film makes it easy to understand why nuns are so mean. He explains: You know, the outfits might have something to do with that. Forget about the fact that they never have sex. If you give me a chance of no sex or having to wear that outfit the rest of my life, I would definitely take the no sex ...
Napadungaw ako sa bintana ng aking sinasakyan, ramdam ko ang malakas na hangin tumatama sa aking cute na mukha, ipinikit ko ang aking mga mata, huminga ng malamin at inisip ang aking magiging asawa. Napangiti ako sa aking nakikita, isang napakagandang babaeng gumaganti din ng halik (hehehe, biro lang), isang napakagandang babaeng gumaganti din ng matamis na ngiti sa akin na parang nagsasabi ng Huwag kang matakot, kasama mo naman ako. Sa ganun paraan palang ay alam ko na ang mga kasagotan sa aking mga katanongan. Pagkatapos nun ay nasabi ko sa aking sarili Oo, sigurado na ako at handa na. sumagot naman ang aking diaw This is it! ...
HOUSTON, TEXAS, Oct. 22, 2012 (GLOBE NEWSWIRE) -- Halcón Resources Corporation (NYSE: HK) (Halcón or the Company) today announced that it has entered into a privately negotiated definitive agreement with Petro-Hunt, L.L.C.
獨特毛孔淨化配方能重現平整無瑕的細緻膚質。蘋果柔白酸、白柳樹皮萃取能深入毛孔溶解粉刺,暢通毛孔。角質調理因子可以調控皮脂分泌,同時預防角質阻塞毛孔及粉刺形成。毛孔調控酵素有效緊緻粗大毛孔。pH5.5親膚弱酸性,不傷肌膚,敏弱肌膚亦適用。 使用方法︰於臉部清潔後使用,取適量塗抹於粉刺較多部位,如T字及下巴,並用指腹輕柔按摩至吸收,即可接續一般保養程序。本產品使用後不需再洗臉。
We report here the complete EM structure of a lactococcal phage determined by single-particle analysis. Phage p2 is the flagship of the 936 group, which are the most prevalent lactococcal phages in industrial dairy fermentations worldwide. This phage is highly virulent and requires Ca2+ to infect specific L. lactis cells, which is in contrast with the less prevalent lactococcal phages belonging to the P335 subgroup II (e.g., TP901-1 and Tuc2009) (15).. The shape of p2 icosahedral capsid is comparable to that of other siphophages. The capsid pentons are, however, more prominent than those of phage TP901-1 but akin to those of coliphage HK97. In contrast, the size of the p2 portal protein (ORF-4) is among the smallest, particularly in comparison to those of phage TP901-1 and Bacillus phage SPP1 (Table 3). The two putative head completion proteins of p2 (ORF-8 and ORF-9) are comparable to those of phage SPP1. The fit of the SPP1 portal and head completion protein 1 (gp15) into the p2 connector EM ...
Looking for online definition of Nun's murmur in the Medical Dictionary? Nun's murmur explanation free. What is Nun's murmur? Meaning of Nun's murmur medical term. What does Nun's murmur mean?
HOLLYWOOD-Horror, I love a great horror movie and as of late I have not been that entertained to say the least. Look, I think you can absolutely make a great movie with a PG-13 rating, but you are limited to a degree with what you can do. When you implement the R-rating, you are not limited, but at the same time you dont have to be excessive with the gore. It is all about the element of suspense, and The Nun has plenty going for it.. Now, many of you might be asking me if you need to see The Conjuring 2 before venturing into the theater to see The Nun. The answer to that question is: no! This is an origin tale, if anything, watching The Conjuring 2 will make you fear The Nun aka Valek who is the main antagonist in that movie. The characters presence alone is spooky as hell. However, if you have knowledge of The Conjuring and The Conjuring 2, the movie The Nun ties in well with those two movies.. There are things that really work with this horror flick. 1) James Wan is on board ...
A watchdog group within the Vatican said they plan a major crackdown for nuns in the United States, accusing them of taking positions that undermine Roman Catholic traditions and teachings.
Terrify in our The Nun Mask this Halloween. Valak is Coming... Fast Delivery. Free Delivery on Eligible Orders. Shop In Time For October 31st.
Buy The Lieutenant Nun Transgenderism, Lesbian Desire, and Catalina De Erauso by Sherry Velasco at TextbookX.com. ISBN/UPC: 9780292787469. Save an average of 50% on the marketplace.
Hk Kd, Wholesale Various High Quality Hk Kd Products from Global Hk Kd Suppliers and Hk Kd Factory,Importer,Exporter at Alibaba.com.
Dengan sik disangka-sangka... saya ari tok tek main youcam di lappy. Ngambik lah gamba tek nak, rasa dirik kiut... bila saya nangga date paling last saya ngambik gamba makey youcam tok tek ehh, guess what!!!!!!!!!!!! It was exactlty this same date last year... heeeee... Pic A (last year), Pic B (just now)... xda banyak beza pun saya setahun yang lepas. Haikal makin besar and saya pun makin gemssss meaning gemoks. Apa-apa pun saya okay. Ehh... sik okay. Ada batu karang dalam perut. Sapa suroh sik jaga makan. Next week jumpa Dr. Ong agik. Molah appointment for operation. Susah2 gi hospital gomen jak kelak. Mun di limbang xda, di miri jak. Xmok saya nak operate di labuan tok. Doktor pun xda suggest operate ctok. Nak dianta nya saya ke KK nun. xde makne nak bekorek perut di KK nun. Bagus gik di tempat dirik. Ada mak k nulong nangga Haikal. Ngantok dah. Esokk lusak cita agik. KBAI ...
My partner Detective _____ and I, Detective _____, responded to _____ Starboard Street to investigate an accidental fall of a female with possible suspicious circumstances. We presumed that the incident originally occurred at approximately 0851 hours, which was the time of the call to 911 from the residence. The female was identified as Riva Pine…
Nice condition Armson OEG Red Dot Reflex sight. This one is an older model designed for the HK 90 series weapons / HK claw mount. The tritium can be
含多種植物萃取物的活性成分及精油,有效減少衰老痕跡,同時吸走肌膚污垢,令肌膚即時回復明亮柔嫰,淨白剔透。
L SUB 4000 A is a flexible 18 double vented bandpass subwoofer. Its powerful low end - ranging from 30 to 100 Hertz - is totally and powerfully impressive.
... bacteriophage hk022 MeSH B04.123.150.800.230 - bacteriophage lambda MeSH B04.123.205.200 - bacteriophage hk022 MeSH B04.123. ... bacteriophage t7 MeSH B04.280.090.800 - siphoviridae MeSH B04.280.090.800.200 - bacteriophage hk022 MeSH B04.280.090.800.230 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ...
Coliphage HK022, alone among the known lambdoid phages, does not encode an analog to lambda N. Instead, it promotes ... The sequences of the rrn BOXA sites are more similar to the bacteriophage consensus than is that of lambda, and they bind NusB- ... put sites act in cis to promote readthrough of downstream terminators in the absence of all HK022 proteins. The put transcripts ... Although the boxB sequence is not well conserved in other bacteriophages of the lambda family, most of these phages encode ...
Bacteriophage Lambda binds to an E. coli cell by means of its J protein in the tail tip. The J protein interacts with the ... with the exception of phage HK022 The genome contains 48,490 base pairs of double-stranded, linear DNA, with 12-base single- ... Burz DS, Beckett D, Benson N, Ackers GK (July 1994). "Self-assembly of bacteriophage lambda cI repressor: effects of single- ... doi:10.1016/j.virol.2015.02.010 Campbell, A.M. Bacteriophages. In: Neidhardt, FC et al. (1996) Escherichia coli and Salmonella ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages.. Juhala RJ1, ... We report the complete genome DNA sequences of HK97 (39,732 bp) and HK022 (40,751 bp), double-stranded DNA bacteriophages of ... We give examples for the generalized recombination genes of HK97, HK022 and P22, and for a putative headtail adaptor protein of ... Pittsburgh Bacteriophage Institute, University of Pittsburgh, PA 15260, USA.. Abstract. ...
1991b Genetic analysis of the cIII gene of bacteriophage HK022. J. Bacteriol. 173: 810-815. ... 1984 Control of bacteriophage lambda CII activity by bacteriophage and host functions. J. Bacteriol. 159: 238-242. ... 1981 Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and CII protein stability. Cell 24: 225-233. ... 1985 The OR control system of bacteriophage lambda: a physical-chemical model for gene regulation. J. Mol. Biol. 181: 211-230. ...
Perez, M. (2017). Using the bacteriophage HK022 nun protein to selectively control the expression of a targeted gene. Presented ... Freeman, C. (2017). Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. Presented at 47th Annual WKU ... Guernsey, E. (2017). Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. Presented at 47th Annual WKU ... Tomko, M. (2017). Collection, isolation, and analysis of arthrobacter bacteriophages Birdperson and Djoodi. Presented at 47th ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Bacteriophage-enhanced sporulation: comparison of spore-converting bacteriophages PMB12 and SP10. J. Bacteriol.172:1948-1953. ... Bacteriophages, Transposons, and Plasmids. Genomic Analysis of Clostridium perfringens Bacteriophage φ3626, Which Integrates ... or bacteriophages (52). In other clostridial species, toxins are known to be bacteriophage encoded; prominent examples are the ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Ferguson, P., and Coombs, D. (2000). Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail. J. Mol. Biol. ... Taxonomic differentiation of 101 lactococcal bacteriophages and characterization of bacteriophages with unusually large genomes ... Bacterial Strains, Bacteriophage and Culture Conditions. Dairy and non-dairy Lactococcus strains were supplied by the TFRC- ...
2000) Genetic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. ... 1988) Bacteriophage P22. in The bacteriophages. ed Calendar R. (Plenum Press, New York, N.Y), pp 647-682. ... 1992) Evolution of dsDNA tailed bacteriophage genomes. in Seminars in virology. ed Koonin E. (Academic Press, London, United ... 1994) A segment of the phage HK022 chromosome is a mosaic of other lambdoid chromosomes. Nucleic Acids Res. 22:354-356. ...
Bacteriophage Lambda nut "spacer" sequences affect HK022 Nun-mediated transcription arrest Washburn, R. S.; Gottesman, M. E. ... QUANTIFICATION OF BACTERIOPHAGES OF BACTEROIDES FRAGILIS IN ENVIRONMENTAL WATER SAMPLES OF SEINE RIVER ... Analysis of some phenotypic traits of feces-borne temperate lambdoid bacteriophages from different immunity groups: a high ...
2000) Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. ... We isolated a bacteriophage, ΦK1-5, that is able to infect and grow on either K1 or K5 strains of E. coli. It appears that its ... 1996) Bacteriophage T4 host range is expanded by duplications of a small domain of the tail fiber adhesion. J. Mol. Biol. 258: ... Bacteriophage K1-5 Encodes Two Different Tail Fiber Proteins, Allowing It To Infect and Replicate on both K1 and K5 Strains of ...
"Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of ... Genomic Sequences of Bacteriophages HK97 and HK022: Pervasive Genetic Mosaicism in the Lambdoid Bacteriophages ... Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains. Krylov, V.; Miller, S ... Ambivalent bacteriophages were found in species other than T-even phages and were similar in morphotype to lambdoid and other E ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol 299: ... Bacteriophage λ and its genetic neighborhood, p 409-447. In Calendar R (ed), The bacteriophages, 2nd ed. Oxford University ... Control mechanisms in dsDNA bacteriophage assembly, p 15-91. In Calendar R (ed), The bacteriophages, vol 1. Plenum Press, New ... Maturation of bacteriophage SPP1 DNA: limited precision in the sizing of mature bacteriophage genomes. J Virol 37:832-835. ...
Specificity determinants in the attachment sites of bacteriophages HK022 and lambda. Journal of bacteriology. 1990;172(11):6540 ... a) S10 is moved by flanking it by HK022 attL and attR sites. Upon transient expression of phage recombinases a DNA circle ... the S10 locus was surrounded by attL and attR sites from phage HK022 flanked respectively by the 5 and 3 parts of the β- ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Iino, T., and, K. Kutsukake. 1981. Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment ... Kamp, D., and, R. Kahmann. 1981. The relationship of two invertible segments in bacteriophage Mu and Salmonella typhimurium DNA ... Affiliations: 1: Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Bacteriophage λ development: temporal switches and the choice of lysis or lysogeny. Annu. Rev. Genet. 2: 26- 30.. ... rexB of bacteriophage lambda is an anti-cell death gene. Proc. Natl. Acad. Sci. USA 95: 15481- 15486.. ... Structure and function of the nun gene and the immunity region of the lambdoid phage HK022. J. Mol. Biol. 207: 675- 693.. ...
After the transformants were screened for phage resistance, one clone was found to exclude HK022 and a (lambda)(q)80 cor ... Lysogens of bacteriophage HK239 have the ability to exclude a wide variety of phage, including (lambda), T4rII, Plvir, P2, and ... In the lytic lifestyle, the bacteriophage infects a cell, uses the hosts cellular machinery to replicate, and lyses the cell ... In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. Most of the ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive geneti mosaicism in the lambdoid bacteriophages. ... Immunity in bacteriophage lambda (and related bacteriophages) is conferred by a short and specific region of the bacteriophage ... Bacteriophage lambda has a dsDNA genome of 48,502 bp. Late replication of bacteriophage lambda is designed to produce many ... Recombination of bacteriophage lambda in recD mutants of Escherichia coli. Recombination of bacteriophage lambda in recD ...
... bacteriophage hk022 MeSH B04.123.150.800.230 - bacteriophage lambda MeSH B04.123.205.200 - bacteriophage hk022 MeSH B04.123. ... bacteriophage t7 MeSH B04.280.090.800 - siphoviridae MeSH B04.280.090.800.200 - bacteriophage hk022 MeSH B04.280.090.800.230 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ...
We found that bacteriophages have unique TUD patterns, representing genomic signatures that are relatively conserved among ... The well-studied model of tail morphology in bacteriophage classification was used for comparison with nucleotide usage ... Analysis of TUD-based phylogeny indicates that host influences are important in bacteriophage evolution, and phylogenies ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosiacism in the lamboid bacteriophages. J Mol Biol. 2000 ...
Therefore, bacteriophage 933W (and presumably bacteriophage H-19B as well) has evolved an alternative way to autogenously ... domain at the N-terminus of the repressor of phage 933W is nearly identical to that of the non-toxic lambdoid phage HK022, but ... Genes encoding these toxins are located on different lambdoid bacteriophage that lyosgenize this strain. Bacteriophage that ... induction is much higher in stx-encoding bacteriophages than in other related bacteriophages [101]. The increased induction ...
The integrases of phages lambda and HK022 are closely related members of this family, but neither protein efficiently ... Finally, substitution at several positions in lambda integrase with the corresponding HK022-specific amino acids prevents ... and this defect can be suppressed in an allele-specific manner by appropriate substitutions of HK022-specific nucleotides in ... Bacteriophage integrases promote recombination between DNA molecules that carry attachment sites. They are members of a large ...
Acids Res2011392116212910.1093/nar/gkq923306480721062824Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic ... Any bacteriophage encoding Stx is termed an Stx phage, and there is much genotypic and phenotypic diversity within this loosely ... bacteriophage SE1 (86%). Phage BP-933 W (100%). AAG55573.1 AAG55573.1. Salmonella phage ST160 (86%). YP_004123782.1 YP_ ... In this study, 26 genes from the bacteriophage Φ24B were identified by either CMAT or 2D-PAGE as being expressed in E. coli ...
Based on nucleotide and amino acid sequences, a high diversity was found among Salmonella bacteriophages which validate the use ... occurred in just one prophage underscoring the high degree of diversity among Salmonella bacteriophages. ... Bacteriophages are bacterial parasites and are considered the most abundant and diverse biological entities on the planet. ... a 11 prophage genomes (mEp390, mEpX2, HK75, mEpX1, HK633, HK446, mEp235, ENT39118, HK022, HK97 and HK106) was present in ...
Coliphage HK022, alone among the known lambdoid phages, does not encode an analog to lambda N. Instead, it promotes ... The sequences of the rrn BOXA sites are more similar to the bacteriophage consensus than is that of lambda, and they bind NusB- ... put sites act in cis to promote readthrough of downstream terminators in the absence of all HK022 proteins. The put transcripts ... Although the boxB sequence is not well conserved in other bacteriophages of the lambda family, most of these phages encode ...
longitudinal q in Due amounts modelling the Int Recombinase of fraction HK022, 1999 Mol. Kuhstoss and Rao, epub Dirt of the ... protein The recurrent and essential models accumulate bacteriophages that describe to provide from gel-purified model of the ... Integration Function of the Streptomycete Bacteriophage PhiC31, 1991 J. A gene for transcription cell and an polynomial cycle ...
Kuhstoss and Rao, ebook sound theatre thoughts on the radio of the Integration Function of the Streptomycete Bacteriophage ... ebook sound theatre thoughts on the radio play 2011 in simultaneous mites using the Int Recombinase of research HK022, 1999 Mol ...
Position and direction of strand exchange in bacteriophage HK022 integration. Forex bre REFERENCE Brre Forex bre melanogaster ...
Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol. ... Hendrix RW: Bacteriophages: evolution of the majority. Theor Popul Biol. 2002, 61 (4): 471-480. 10.1006/tpbi.2002.1590. ... Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. ...
Phamerator represents a useful tool for comparative genomic analysis and comparative representations of bacteriophage genomes. ... Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol. ... Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting ...
Phage HK022 Nun protein has been an active area of research. We have just begun to understand how Nun interacts with E. coli ... Regulation of transcription termination in E. coli and bacteriophage. *Repair of DNA damage in prokaryotes and eukaryotes ... 3. Kim, H.C., Washburn, R.S, and Gottesman M.E. : (2006) Role of E. coli NusA in Phage HK022 Nun-mediated Transcription ... 1. Kim, H.C. and Gottesman, M.E.: (2004) Transcription Termination by Phage HK022 Nun Is Facilitated by C-terminal Lysine ...
Genomic sequences of bacteriophages HK97 and HK022: Pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... The DNA spooling inside the capsids is shown in (a) phi29 bacteriophage; (b) ε 15 bacteriophage; and (c) T7. The toroid formed ... DNA conformation during genome packaging T7 bacteriophage Jose L. Carrascosa and co-workers Toroid (b) ε 15 bacteriophage Wah ... Structural remodeling of bacteriophage T4 and host membranes during infection initiation. Proc Natl Acad Sci U S A. 112(35), ...
... bacteriophages hk022, lambda, T5, BF 23. [0057]The Corticoviridae family comprises icosahedral, lipid-containing, non-enveloped ... bacteriophage is related to the Salmonella typhimurium bacteriophage MB78. The growth of φmF bacteriophage has been tested on ... Such bacteriophage can be selected as described previously. [0065]Determining an effective amount of the bacteriophage to be ... 0046]We use any bacteriophage, preferably bacteriophages that are virulent for the bacterial strain used in the method. As an ...
  • We report the complete genome DNA sequences of HK97 (39,732 bp) and HK022 (40,751 bp), double-stranded DNA bacteriophages of Escherichia coli and members of the lambdoid or lambda-like group of phages. (nih.gov)
  • Although the existence of bacteriophages infecting C. perfringens has been reported ( 43 ) and a certain phenotypic effect of temperate phages of this organism has demonstrated ( 61 ), we were surprised to find that no sequences or other molecular data on C. perfringens phages were available, except for a preliminary mapping of the integration sites of two phages ( 7 ). (asm.org)
  • Shaburova, O. 2006-03-15 00:00:00 A study was made of several bacteriophages (including phages U2 and LB related to T-even phages of Escherichia coli) that grow both on E. coli K12 and on some Salmonella strains. (deepdyve.com)
  • Ambivalent bacteriophages were found in species other than T-even phages and were similar in morphotype to lambdoid and other E. coli phages. (deepdyve.com)
  • Since original isolation of Stx1 and Stx2 lambdoid bacteriophage from Shiga toxin encoding E. coli (STEC), many other Stx-encoding phages have been isolated and their sequences at least partially characterized. (mdpi.com)
  • Analysis of TUD-based phylogeny indicates that host influences are important in bacteriophage evolution, and phylogenies containing both phages and their hosts support their co-evolution. (biomedcentral.com)
  • The integrases of phages lambda and HK022 are closely related members of this family, but neither protein efficiently recombines the attachment sites of the other phage. (semanticscholar.org)
  • Bacteriophages, sometimes simply referred to as phages, are considered the most abundant biological entities on the planet [ 8 ]. (biomedcentral.com)
  • Bacteriophages (phages), which are natural viral predators of bacteria, multiply by infecting specific host bacteria. (prodejce.cz)
  • 1 1.1.1 DNA packaging motor of double-stranded DNA bacteriophages. (b-ok.org)
  • Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. (nih.gov)
  • We give examples for the generalized recombination genes of HK97, HK022 and P22, and for a putative headtail adaptor protein of HK97 and HK022. (nih.gov)
  • Juhala, R.J., M.E. Ford, R.L. Duda, A. Youlton, G.F. Hatfull, and R.W. Hendrix (2000) Genomic sequences of bacteriophages HK97 and HK022: pervasive geneti mosaicism in the lambdoid bacteriophages . (factbites.com)
  • Bacteriophage HK97 is a lambdoid phage with a head assembled from 415 copies of a 42 kDa subunit arranged in an icosahedrally symmetrical lattice with a triangulation number of 7. (factbites.com)
  • The sequence of the nonredundant region of the Salmonella enterica serovar Typhimurium temperate, serotype-converting bacteriophage P22 has been completed. (asm.org)
  • Temperate bacteriophage can adopt either a lytic or a lysogenic lifestyle. (wku.edu)
  • We found that bacteriophages have unique TUD patterns, representing genomic signatures that are relatively conserved among those with similar host range. (biomedcentral.com)
  • Comparative genomic analysis of 142 bacteriophages infecting Salmonella enterica subsp. (biomedcentral.com)
  • Phamerator represents a useful tool for comparative genomic analysis and comparative representations of bacteriophage genomes. (biomedcentral.com)
  • In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. (wku.edu)
  • In contrast to the lytic cycle, which results in immediate bursting of the host bacteria and the release of bacteriophage progeny, the lysogenic cycle involves the maintenance of the phage genome as a part of the host genome for several generations, typically by integrating into host chromosomes or, more rarely, by replicating as low-copy-number phage plasmids (2-4). (prodejce.cz)
  • A virulent double-stranded DNA bacteriophage, ΦK1-5, has been isolated and found to be capable of infecting Escherichia coli strains that possess either the K1 or the K5 polysaccharide capsule. (asm.org)
  • Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. (deepdyve.com)
  • Characterisation of Escherichia coli Infection with a Verocytotoxigenic Bacteriophage . (factbites.com)
  • Kuhstoss and Rao, reconstruction of the knee of the Integration Function of the Streptomycete Bacteriophage PhiC31, 1991 J. A art for recombinase attP-2 and an financial event in Escherichia challenges K-12, 1985 Gene, comparable. (isn-hi.de)
  • Genes encoding these toxins are located on different lambdoid bacteriophage that lyosgenize this strain. (mdpi.com)
  • ΦK5 is a related bacteriophage specific for E. coli strains that display the K5 antigen, a polymer consisting of a repeating structure of 4-linked α- N -acetylglucosamine and β-glucuronic acid ( N -acetyl heparosin). (asm.org)
  • Bacteriophage that separately encode Stx1 and Stx2 have been isolated from various EHEC strains, and these phage are now representative of the Stx-encoding prophages. (mdpi.com)
  • In the lytic lifestyle, the bacteriophage infects a cell, uses the host's cellular machinery to replicate, and lyses the cell to release the phage progeny. (wku.edu)
  • Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. (biomedcentral.com)
  • Bacteriophage lambda is a double-stranded DNA (dsDNA) phage with an isometric head about 50 nm diameter and a flexible tail about 150 nm long (3) ( micrograph). (factbites.com)
  • to superinfection by the same phage due to repression of transcription caused by the resident prophage (See Bacteriophage Lambda ). (factbites.com)
  • Moreover, bacteriophage have evolved extrinsic factors that use ingenious mechanisms to subvert the host transcription process for their own purposes. (rockefeller.edu)
  • After the transformants were screened for phage resistance, one clone was found to exclude HK022 and a (lambda)(q)80 cor homolog. (wku.edu)
  • In the case of bacteriophage lambda , however, the appearance of the plaques is informative. (factbites.com)
  • Immunity in bacteriophage lambda (and related bacteriophages ) is conferred by a short and specific region of the bacteriophage chromosome. (factbites.com)
  • Different bacteriophage lambda containing the immunity regions of related lambdoid phage were very useful in the genetic studies of the immunity region. (factbites.com)
  • Lambda is a double-stranded DNA bacteriophage of E. coli . (factbites.com)
  • They are still a paradigm for many areas of biology, especially gene expression (See Bacteriophage Lambda ). (factbites.com)
  • Trans cooperativity by a split DNA recombinase: the central and catalytic domains of bacteriophage lambda integrase cooperate in cleaving DNA substrates when the two domains are not covalently linked. (semanticscholar.org)
  • A majority of the phamilies, 4330 out of 5796 (74.7%), occurred in just one prophage underscoring the high degree of diversity among Salmonella bacteriophages. (biomedcentral.com)
  • For the first time, the nucleotide sequence of a bacteriophage infecting Clostridium species was determined. (asm.org)
  • The well-studied model of tail morphology in bacteriophage classification was used for comparison with nucleotide usage patterns. (biomedcentral.com)
  • Genetic control of bacteriophage lysogeny and lysis. (uchc.edu)
  • Bacteriophages attach to specific outer membrane proteins, which surround the rigid peptidoglycan layer of the bacterial cell wall. (factbites.com)
  • Bacteriophages , like bacteria, are very common in all natural environments and are directly related to the numbers of bacteria present. (factbites.com)
  • Wide spread resistance against antibiotics has prompted a renewed surge of interest in bacteriophages which are viruses capable of infecting and sometimes killing bacteria, as safe and effective therapy alternatives [ 7 ]. (biomedcentral.com)
  • Eukaryotic viruses and bacteriophages exist in numerous forms and are capable of infecting disparate hosts. (biomedcentral.com)
  • mean reconstruction of in necessary data collapsing the Int Recombinase of DNA HK022, 1999 Mol. (isn-hi.de)
  • It has been reasoned that the similarities of some toxins of C. perfringens with toxins found in other organisms are due to horizontal gene transfer based on conjugative plasmids, transposons, or bacteriophages ( 52 ). (asm.org)
  • Bacteriophage integrases promote recombination between DNA molecules that carry attachment sites. (semanticscholar.org)
  • Unusual structure of the attB site of the site-specific recombination system of Lactobacillus delbrueckii bacteriophage mv4. (semanticscholar.org)
  • Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting challenges in their annotation, comparative analysis, and representation. (biomedcentral.com)
  • The first step of bacteriophage infection is adsorption, or the attachment of the virus to the bacterial surface. (factbites.com)
  • Each bacteriophage was probably specialized to an environment, and the closer the laboratory conditions were to the environment, the more effective the infection was. (factbites.com)
  • 2. The method according to claim 1, wherein the concentration of said antibiotic in the medium is in the range which causes about 1% to about 99% inhibition of the growth of said bacterial strain in the absence of said bacteriophage. (patentsencyclopedia.com)
  • Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. (wku.edu)
  • Bacteriophages are the most abundant organisms in the biosphere and play major roles in the ecological balance of microbial life. (factbites.com)
  • Bacteriophages are bacterial parasites and are considered the most abundant and diverse biological entities on the planet. (biomedcentral.com)
  • These polysaccharide coats also act as recognition sites for bacteriophages, which often carry tail spikes that contain polysaccharide depolymerization activities. (asm.org)
  • Tail morphology forms the basis for bacteriophage classification into 3 separate families: Myoviridae (contractile tails), Podoviridae (short tail stubs), and Siphoviridae (long tails) [ 1 ]. (biomedcentral.com)
  • Phage HK022 Nun protein has been an active area of research. (columbia.edu)
  • Preliminary evidence suggests that this region is highly homologous to the conversion- att-int region from Shigella flexneri bacteriophage SfV ( 32 ). (asm.org)
  • Bacteriophage.The bacteriophages used in this study are listed in Table 1. (prodejce.cz)