Siphoviridae
Bacteriophage lambda
Cryoelectron Microscopy
Bacteriophage T4
Bacteriophage T7
Lysogeny
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
T-Phages
A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.
Bacteriophage mu
A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.
Bacteriophage phi 6
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bacteriophage phi X 174
Bacteriophage P2
Bacteriophage M13
Bacteriophage T3
Bacteriophage Typing
Bacteriophage P1
Salmonella Phages
RNA Phages
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Bacteriolysis
Bacteriophage PRD1
Pseudomonas Phages
Bacillus Phages
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Mutation
Viral Tail Proteins
Levivirus
Adsorption
DNA Packaging
Plasmids
Prophages
Inovirus
Genes
DNA-Directed RNA Polymerases
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
Genetics, Microbial
Recombination, Genetic
Amino Acid Sequence
Attachment Sites, Microbiological
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Virus Replication
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Transduction, Genetic
Cloning, Molecular
DNA, Single-Stranded
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Centrifugation, Density Gradient
Activity of coliphage HK022 excisionase (Xis) in the absence of DNA binding. (1/10)
A mutated excisionase (Xis) protein of coliphage HK022 whose single Cys residue was replaced by Ser does not bind to its two tandem binding sites (X1, X2) on the P arm of attR. Despite its DNA-binding inability the protein showed 30% excision activity of the wild type Xis both in vitro and in vivo. This partial activity is attributed to the interaction of Xis with integrase that is retained in the mutant protein. This protein-protein interaction occurs in the absence of DNA binding. (+info)Suppression of factor-dependent transcription termination by antiterminator RNA. (2/10)
Nascent transcripts of the phage HK022 put sites modify the transcription elongation complex so that it terminates less efficiently at intrinsic transcription terminators and accelerates through pause sites. We show here that the modification also suppresses termination in vivo at two factor-dependent terminators, one that depends on the bacterial Rho protein and a second that depends on the HK022-encoded Nun protein. Suppression was efficient when the termination factors were present at physiological levels, but an increase in the intracellular concentration of Nun increased termination both in the presence and absence of put. put-mediated antitermination thus shows no apparent terminator specificity, suggesting that put inhibits a step that is common to termination at the different types of terminator. (+info)Solution structure and stability of the full-length excisionase from bacteriophage HK022. (3/10)
Heteronuclear high-resolution NMR spectroscopy was employed to determine the solution structure of the excisionase protein (Xis) from the lambda-like bacteriophage HK022 and to study its sequence-specific DNA interaction. As wild-type Xis was previously characterized as a generally unstable protein, a biologically active HK022 Xis mutant with a single amino acid substitution Cys28-->Ser was used in this work. This substitution has been shown to diminish the irreversibility of Xis denaturation and subsequent degradation, but does not affect the structural or thermodynamic properties of the protein, as evidenced by NMR and differential scanning calorimetry. The solution structure of HK022 Xis forms a compact, highly ordered protein core with two well-defined alpha-helices (residues 5-11 and 18-27) and five beta-strands (residues 2-4, 30-31, 35-36, 41-44 and 48-49). These data correlate well with 1H2O-2H2O exchange experiments and imply a different organization of the HK022 Xis secondary structure elements in comparison with the previously determined structure of the bacteriophage lambda excisionase. Superposition of both Xis structures indicates a better correspondence of the full-length HK022 Xis to the typical 'winged-helix' DNA-binding motif, as found, for example, in the DNA-binding domain of the Mu-phage repressor. Residues 51-72, which were not resolved in the lambda Xis, do not show any regular structure in HK022 Xis and thus appear to be completely disordered in solution. The resonance assignments have shown, however, that an unusual connectivity exists between residues Asn66 and Gly67 owing to asparagine-isoaspartyl isomerization. Such an isomerization has been previously observed and characterized only in eukaryotic proteins. (+info)Transcription termination by phage HK022 Nun is facilitated by COOH-terminal lysine residues. (4/10)
The 109-amino acid Nun protein of prophage HK022 excludes superinfecting bacteriophage lambda by blocking transcription elongation on the lambda chromosome. Multiple interactions between Nun and the transcription elongation complex are involved in this reaction. The Nun NH(2)-terminal arginine-rich motif binds BOXB sequence in nascent lambda transcripts, whereas the COOH terminus binds RNA polymerase and contacts DNA template. Nun Trp(108) is required for interaction with DNA and transcription arrest. We analyzed the role of the adjacent Lys(106) and Lys(107) residues in the Nun reaction. Substitution of the lysine residues with arginine (K106R/K107R) had no effect on transcription arrest in vitro or in vivo. Nun K106A/K107A was partially active, whereas Nun K106D/K107D was defective in vitro and failed to exclude lambda. All mutants bound RNA polymerase and BOXB. In contrast to Nun K106R/K107R and K106A/K107A, Nun K106D/K107D did not cross-link DNA template. These results suggest that transcription arrest is facilitated by electrostatic interactions between positively charged Nun residues Lys(106) and Lys(107) and negatively charged DNA phosphate groups. These may assist intercalation of Trp(108) into template. (+info)Phosphorylation of the integrase protein of coliphage HK022. (5/10)
(+info)In vitro reconstitution and substrate specificity of a lantibiotic protease. (6/10)
(+info)Overexpression of phage HK022 Nun protein is toxic for Escherichia coli. (7/10)
(+info)Inhibition of a transcriptional pause by RNA anchoring to RNA polymerase. (8/10)
(+info)
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List of MeSH codes (B04)
... bacteriophage hk022 MeSH B04.123.150.800.230 - bacteriophage lambda MeSH B04.123.205.200 - bacteriophage hk022 MeSH B04.123. ... bacteriophage t7 MeSH B04.280.090.800 - siphoviridae MeSH B04.280.090.800.200 - bacteriophage hk022 MeSH B04.280.090.800.230 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ...
Lambda phage
Bacteriophage Lambda binds to an E. coli cell by means of its J protein in the tail tip. The J protein interacts with the ... with the exception of phage HK022 The genome contains 48,490 base pairs of double-stranded, linear DNA, with 12-base single- ... Burz DS, Beckett D, Benson N, Ackers GK (July 1994). "Self-assembly of bacteriophage lambda cI repressor: effects of single- ... doi:10.1016/j.virol.2015.02.010 Campbell, A.M. Bacteriophages. In: Neidhardt, FC et al. (1996) Escherichia coli and Salmonella ...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. - PubMed - NCBI
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages.. Juhala RJ1, ... We report the complete genome DNA sequences of HK97 (39,732 bp) and HK022 (40,751 bp), double-stranded DNA bacteriophages of ... We give examples for the generalized recombination genes of HK97, HK022 and P22, and for a putative headtail adaptor protein of ... Pittsburgh Bacteriophage Institute, University of Pittsburgh, PA 15260, USA.. Abstract. ...
Stochastic Kinetic Analysis of Developmental Pathway Bifurcation in Phage λ-Infected Escherichia coli Cells | Genetics
1991b Genetic analysis of the cIII gene of bacteriophage HK022. J. Bacteriol. 173: 810-815. ... 1984 Control of bacteriophage lambda CII activity by bacteriophage and host functions. J. Bacteriol. 159: 238-242. ... 1981 Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and CII protein stability. Cell 24: 225-233. ... 1985 The OR control system of bacteriophage lambda: a physical-chemical model for gene regulation. J. Mol. Biol. 181: 211-230. ...
Class of 2016 | Western Kentucky University
Perez, M. (2017). Using the bacteriophage HK022 nun protein to selectively control the expression of a targeted gene. Presented ... Freeman, C. (2017). Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. Presented at 47th Annual WKU ... Guernsey, E. (2017). Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. Presented at 47th Annual WKU ... Tomko, M. (2017). Collection, isolation, and analysis of arthrobacter bacteriophages Birdperson and Djoodi. Presented at 47th ...
Sequence of the Genome of the Temperate, Serotype-Converting,Pseudomonas aeruginosa Bacteriophage D3 | Journal of Bacteriology
The greatest sequence similarity was found in the morphogenesis genes to coliphages HK022 and HK97. Among the ORFs was ... BACTERIOPHAGES. Sequence of the Genome of the Temperate, Serotype-Converting,Pseudomonas aeruginosa Bacteriophage D3. Andrew M ... Temperate bacteriophage D3, a member of the virus familySiphoviridae, is responsible for serotype conversion in its host, ... Sequence of the Genome of the Temperate, Serotype-Converting,Pseudomonas aeruginosa Bacteriophage D3 ...
Frontiers | Phages of non-dairy lactococci: isolation and characterization of ΦL47, a phage infecting the grass isolate...
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Ferguson, P., and Coombs, D. (2000). Pulse-chase analysis of the in vivo assembly of the bacteriophage T4 tail. J. Mol. Biol. ... Taxonomic differentiation of 101 lactococcal bacteriophages and characterization of bacteriophages with unusually large genomes ... Bacterial Strains, Bacteriophage and Culture Conditions. Dairy and non-dairy Lactococcus strains were supplied by the TFRC- ...
Stability and Instability in the Lysogenic State of Phage Lambda | Journal of Bacteriology
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Bacteriophages, Transposons, and Plasmids. Stability and Instability in the Lysogenic State of Phage Lambda. John W. Little, ... Bacteriophage lambda cro mutations: effects on activity and intracellular degradation. Proc. Natl. Acad. Sci. U. S. A. 83:8829- ... Behavior of λ bacteriophage in a recombination deficient strain of E. coli. Virology 1:283-293. ...
PHAGEN (phages): Topics by WorldWideScience.org
Bacteriophage Lambda nut "spacer" sequences affect HK022 Nun-mediated transcription arrest Washburn, R. S.; Gottesman, M. E. ... QUANTIFICATION OF BACTERIOPHAGES OF BACTEROIDES FRAGILIS IN ENVIRONMENTAL WATER SAMPLES OF SEINE RIVER ... Analysis of some phenotypic traits of feces-borne temperate lambdoid bacteriophages from different immunity groups: a high ...
Bacteriophage K1-5 Encodes Two Different Tail Fiber Proteins, Allowing It To Infect and Replicate on both K1 and K5 Strains of...
2000) Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. ... We isolated a bacteriophage, ΦK1-5, that is able to infect and grow on either K1 or K5 strains of E. coli. It appears that its ... 1996) Bacteriophage T4 host range is expanded by duplications of a small domain of the tail fiber adhesion. J. Mol. Biol. 258: ... Bacteriophage K1-5 Encodes Two Different Tail Fiber Proteins, Allowing It To Infect and Replicate on both K1 and K5 Strains of ...
Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of...
"Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of ... Genomic Sequences of Bacteriophages HK97 and HK022: Pervasive Genetic Mosaicism in the Lambdoid Bacteriophages ... Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains. Krylov, V.; Miller, S ... Ambivalent bacteriophages were found in species other than T-even phages and were similar in morphotype to lambdoid and other E ...
DNA Packaging and Genomics of the Salmonella 9NA-Like Phages | Journal of Virology
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol 299: ... Bacteriophage λ and its genetic neighborhood, p 409-447. In Calendar R (ed), The bacteriophages, 2nd ed. Oxford University ... Control mechanisms in dsDNA bacteriophage assembly, p 15-91. In Calendar R (ed), The bacteriophages, vol 1. Plenum Press, New ... Maturation of bacteriophage SPP1 DNA: limited precision in the sizing of mature bacteriophage genomes. J Virol 37:832-835. ...
ASMscience | Studying Evolution Using
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Iino, T., and, K. Kutsukake. 1981. Trans-acting genes of bacteriophages P1 and Mu mediate inversion of a specific DNA segment ... Kamp, D., and, R. Kahmann. 1981. The relationship of two invertible segments in bacteriophage Mu and Salmonella typhimurium DNA ... Affiliations: 1: Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh ...
ASMscience | Lysogeny, Prophage Induc
Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... Bacteriophage λ development: temporal switches and the choice of lysis or lysogeny. Annu. Rev. Genet. 2: 26- 30.. ... rexB of bacteriophage lambda is an anti-cell death gene. Proc. Natl. Acad. Sci. USA 95: 15481- 15486.. ... Structure and function of the nun gene and the immunity region of the lambdoid phage HK022. J. Mol. Biol. 207: 675- 693.. ...
"Identifying Exclusion Genes of Bacteriophage HK239" by Ali Wright
After the transformants were screened for phage resistance, one clone was found to exclude HK022 and a (lambda)(q)80 cor ... Lysogens of bacteriophage HK239 have the ability to exclude a wide variety of phage, including (lambda), T4rII, Plvir, P2, and ... In the lytic lifestyle, the bacteriophage infects a cell, uses the hosts cellular machinery to replicate, and lyses the cell ... In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. Most of the ...
List of MeSH codes (B04) - Wikipedia
... bacteriophage hk022 MeSH B04.123.150.800.230 - bacteriophage lambda MeSH B04.123.205.200 - bacteriophage hk022 MeSH B04.123. ... bacteriophage t7 MeSH B04.280.090.800 - siphoviridae MeSH B04.280.090.800.200 - bacteriophage hk022 MeSH B04.280.090.800.230 - ... bacteriophage p1 MeSH B04.123.205.305 - bacteriophage p2 MeSH B04.123.205.320 - bacteriophage phi x 174 MeSH B04.123.205.350 - ... bacteriophage n4 MeSH B04.123.150.700.070 - bacteriophage p22 MeSH B04.123.150.700.100 - bacteriophage t3 MeSH B04.123.150.700. ...
Toxins | Free Full-Text | Shiga Toxin: Expression, Distribution, and Its Role in the Environment | HTML
Therefore, bacteriophage 933W (and presumably bacteriophage H-19B as well) has evolved an alternative way to autogenously ... domain at the N-terminus of the repressor of phage 933W is nearly identical to that of the non-toxic lambdoid phage HK022, but ... Genes encoding these toxins are located on different lambdoid bacteriophage that lyosgenize this strain. Bacteriophage that ... induction is much higher in stx-encoding bacteriophages than in other related bacteriophages [101]. The increased induction ...
Recognition of core binding sites by bacteriophage integrases. - Semantic Scholar
The integrases of phages lambda and HK022 are closely related members of this family, but neither protein efficiently ... Finally, substitution at several positions in lambda integrase with the corresponding HK022-specific amino acids prevents ... and this defect can be suppressed in an allele-specific manner by appropriate substitutions of HK022-specific nucleotides in ... Bacteriophage integrases promote recombination between DNA molecules that carry attachment sites. They are members of a large ...
Identification of genes expressed in cultures of E. coli lysogens carrying the Shiga toxin-encoding prophage Φ24B
Acids Res2011392116212910.1093/nar/gkq923306480721062824Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic ... Any bacteriophage encoding Stx is termed an Stx phage, and there is much genotypic and phenotypic diversity within this loosely ... bacteriophage SE1 (86%). Phage BP-933 W (100%). AAG55573.1 AAG55573.1. Salmonella phage ST160 (86%). YP_004123782.1 YP_ ... In this study, 26 genes from the bacteriophage Φ24B were identified by either CMAT or 2D-PAGE as being expressed in E. coli ...
DeCS
Enterobacteria phage HK022. HK022, Bacteriophage. HK022, Enterobacteria phage. Tree number(s):. B04.123.150.800.200. B04.123. ... Bacteriophage HK022 - Preferred Concept UI. M0016444. Scope note. A tentative species in the genus lambda-like viruses, family ... Bacteriophage HK022 Entry term(s). Enterobacteria phage HK022 HK022, Bacteriophage HK022, Enterobacteria phage ...
Comparative genomic analysis of 142 bacteriophages infecting Salmonella enterica subsp. enterica | BMC Genomics | Full Text
Based on nucleotide and amino acid sequences, a high diversity was found among Salmonella bacteriophages which validate the use ... occurred in just one prophage underscoring the high degree of diversity among Salmonella bacteriophages. ... Bacteriophages are bacterial parasites and are considered the most abundant and diverse biological entities on the planet. ... a 11 prophage genomes (mEp390, mEpX2, HK75, mEpX1, HK633, HK446, mEp235, ENT39118, HK022, HK97 and HK106) was present in ...
Epub Dirt Music
longitudinal q in Due amounts modelling the Int Recombinase of fraction HK022, 1999 Mol. Kuhstoss and Rao, epub Dirt of the ... protein The recurrent and essential models accumulate bacteriophages that describe to provide from gel-purified model of the ... Integration Function of the Streptomycete Bacteriophage PhiC31, 1991 J. A gene for transcription cell and an polynomial cycle ...
Why Iptv: Interactivity, Technologies, Services 2008
Kuhstoss and Rao, Why of the Integration Function of the Streptomycete Bacteriophage PhiC31, 1991 J. A kb for intro study and ... Services 2008 in other molecules cloning the Int Recombinase of orientation HK022, 1999 Mol. ... into the Klebsiella flexibility product via Bacteriophage P4 Site-Specific Recombination, 1983 J. Ow and Medberry, Genome ...
Forex bre
Phamerator: a bioinformatic tool for comparative bacteriophage genomics | BMC Bioinformatics | Full Text
Phamerator represents a useful tool for comparative genomic analysis and comparative representations of bacteriophage genomes. ... Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol. ... Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting ...
CD73 Cynomolgus HEK293 His - Labshake
SARS Coronavirus Nucleoprotein NP His tag HEK293 - Labshake
Identification and investigation of ORFans in the viral world | BMC Genomics | Full Text
Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol. ... Hendrix RW: Bacteriophages: evolution of the majority. Theor Popul Biol. 2002, 61 (4): 471-480. 10.1006/tpbi.2002.1590. ... Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. ...
Max E. Gottesman, PhD | Graduate School of Arts and Sciences
Phage HK022 Nun protein has been an active area of research. We have just begun to understand how Nun interacts with E. coli ... Regulation of transcription termination in E. coli and bacteriophage. *Repair of DNA damage in prokaryotes and eukaryotes ... 3. Kim, H.C., Washburn, R.S, and Gottesman M.E. : (2006) Role of E. coli NusA in Phage HK022 Nun-mediated Transcription ... 1. Kim, H.C. and Gottesman, M.E.: (2004) Transcription Termination by Phage HK022 Nun Is Facilitated by C-terminal Lysine ...
Biomotors: Linear, Rotation, and Revolution Motion Mechanisms | Peixuan Guo, Zhengyi Zhao | download
Genomic sequences of bacteriophages HK97 and HK022: Pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol. ... The DNA spooling inside the capsids is shown in (a) phi29 bacteriophage; (b) ε 15 bacteriophage; and (c) T7. The toroid formed ... DNA conformation during genome packaging T7 bacteriophage Jose L. Carrascosa and co-workers Toroid (b) ε 15 bacteriophage Wah ... Structural remodeling of bacteriophage T4 and host membranes during infection initiation. Proc Natl Acad Sci U S A. 112(35), ...
PROCESS OF PRODUCTION OF BACTERIOPHAGE COMPOSITIONS AND METHODS IN PHAGE THERAPY FIELD - Patent application
... bacteriophages hk022, lambda, T5, BF 23. [0057]The Corticoviridae family comprises icosahedral, lipid-containing, non-enveloped ... bacteriophage is related to the Salmonella typhimurium bacteriophage MB78. The growth of φmF bacteriophage has been tested on ... Such bacteriophage can be selected as described previously. [0065]Determining an effective amount of the bacteriophage to be ... 0046]We use any bacteriophage, preferably bacteriophages that are virulent for the bacterial strain used in the method. As an ...
Genetic and phenotypic diversity in Burkholderia : contributions by prophage and phage-like elements | BMC Microbiology | Full...
Five of these were spontaneously induced to form bacteriophage particles from B. pseudomallei and B. thailandensis strains and ... Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J Mol Biol. ... Five bacteriophages were isolated and fully sequenced (Table 1A).. Table 1 Sources and descriptions of bacteriophage and ... Isolated and sequenced bacteriophages. Five bacteriophages were isolated from three B. pseudomallei and two B. thailandensis ...
Phage8
- In the lytic lifestyle, the bacteriophage infects a cell, uses the host's cellular machinery to replicate, and lyses the cell to release the phage progeny. (wku.edu)
- After the transformants were screened for phage resistance, one clone was found to exclude HK022 and a (lambda)(q)80 cor homolog. (wku.edu)
- Bacteriophage that separately encode Stx1 and Stx2 have been isolated from various EHEC strains, and these phage are now representative of the Stx-encoding prophages. (mdpi.com)
- The integrases of phages lambda and HK022 are closely related members of this family, but neither protein efficiently recombines the attachment sites of the other phage. (semanticscholar.org)
- Phage HK022 Nun protein has been an active area of research. (columbia.edu)
- Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). (wikipedia.org)
- All characterized lambdoid phages possess an N protein-mediated transcription antitermination mechanism, with the exception of phage HK022 The genome contains 48,490 base pairs of double-stranded, linear DNA, with 12-base single-strand segments at both 5' ends. (wikipedia.org)
- Mycobacteriophages are the most studied of all bacteriophages with 2,413 mycobacteriophages isolated, more than 344 genomes fully sequenced ( http://phagesdb.org/ ) and approximately 223 full phage genome sequences available on GenBank, making the analysis of these phages a model for bacteriophage research. (biomedcentral.com)
Double-stranded DNA bacteriophages2
HK973
- Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. (nih.gov)
- We give examples for the generalized recombination genes of HK97, HK022 and P22, and for a putative headtail adaptor protein of HK97 and HK022. (nih.gov)
- The greatest sequence similarity was found in the morphogenesis genes to coliphages HK022 and HK97. (asm.org)
Temperate2
Bacterial3
- In the lysogenic lifestyle the bacteriophage genome is integrated into the bacterial genome to generate a prophage. (wku.edu)
- Bacteriophages are bacterial parasites and are considered the most abundant and diverse biological entities on the planet. (biomedcentral.com)
- 2. The method according to claim 1, wherein the concentration of said antibiotic in the medium is in the range which causes about 1% to about 99% inhibition of the growth of said bacterial strain in the absence of said bacteriophage. (patentsencyclopedia.com)
Phages4
- Shaburova, O. 2006-03-15 00:00:00 A study was made of several bacteriophages (including phages U2 and LB related to T-even phages of Escherichia coli) that grow both on E. coli K12 and on some Salmonella strains. (deepdyve.com)
- Ambivalent bacteriophages were found in species other than T-even phages and were similar in morphotype to lambdoid and other E. coli phages. (deepdyve.com)
- Since original isolation of Stx1 and Stx2 lambdoid bacteriophage from Shiga toxin encoding E. coli (STEC), many other Stx-encoding phages have been isolated and their sequences at least partially characterized. (mdpi.com)
- Bacteriophages, sometimes simply referred to as phages, are considered the most abundant biological entities on the planet [ 8 ]. (biomedcentral.com)
Genome2
- Here we present a first genome-wide identification and analysis of ORFans in the viral world, with focus on bacteriophages. (biomedcentral.com)
- Phylogenetic comparison of bacteriophages requires whole genome approaches such as dotplot analysis, genome pairwise maps, and gene content analysis. (biomedcentral.com)
Genes1
- Genes encoding these toxins are located on different lambdoid bacteriophage that lyosgenize this strain. (mdpi.com)
Lambda2
- Trans cooperativity by a split DNA recombinase: the central and catalytic domains of bacteriophage lambda integrase cooperate in cleaving DNA substrates when the two domains are not covalently linked. (semanticscholar.org)
- Bacteriophage Lambda binds to an E. coli cell by means of its J protein in the tail tip. (wikipedia.org)
Escherichia2
- A virulent double-stranded DNA bacteriophage, ΦK1-5, has been isolated and found to be capable of infecting Escherichia coli strains that possess either the K1 or the K5 polysaccharide capsule. (asm.org)
- Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. (deepdyve.com)
Lysis1
- Genetic control of bacteriophage lysogeny and lysis. (uchc.edu)
Genomic analysis2
- Comparative genomic analysis of 142 bacteriophages infecting Salmonella enterica subsp. (biomedcentral.com)
- Phamerator represents a useful tool for comparative genomic analysis and comparative representations of bacteriophage genomes. (biomedcentral.com)
Genomes1
- Bacteriophage genomes have mosaic architectures and are replete with small open reading frames of unknown function, presenting challenges in their annotation, comparative analysis, and representation. (biomedcentral.com)
Strains1
- ΦK5 is a related bacteriophage specific for E. coli strains that display the K5 antigen, a polymer consisting of a repeating structure of 4-linked α- N -acetylglucosamine and β-glucuronic acid ( N -acetyl heparosin). (asm.org)
Recombination2
- Bacteriophage integrases promote recombination between DNA molecules that carry attachment sites. (semanticscholar.org)
- Unusual structure of the attB site of the site-specific recombination system of Lactobacillus delbrueckii bacteriophage mv4. (semanticscholar.org)
Transcription1
- Moreover, bacteriophage have evolved extrinsic factors that use ingenious mechanisms to subvert the host transcription process for their own purposes. (rockefeller.edu)
Viruses1
- Wide spread resistance against antibiotics has prompted a renewed surge of interest in bacteriophages which are viruses capable of infecting and sometimes killing bacteria, as safe and effective therapy alternatives [ 7 ]. (biomedcentral.com)
Prophage1
- A majority of the phamilies, 4330 out of 5796 (74.7%), occurred in just one prophage underscoring the high degree of diversity among Salmonella bacteriophages. (biomedcentral.com)
Diversity1
- Comparison of Pemberly and Jeggs: An exploration of bacteriophage diversity. (wku.edu)
Recognition2
- These polysaccharide coats also act as recognition sites for bacteriophages, which often carry tail spikes that contain polysaccharide depolymerization activities. (asm.org)
- Recognition of core binding sites by bacteriophage integrases. (semanticscholar.org)
Shown1
- It is unclear whether all of these putative prophages are active, although one (ΦK96243) was shown to be a productive bacteriophage. (beds.ac.uk)