Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
Infections with bacteria of the genus LISTERIA.
Viruses whose hosts are bacterial cells.
A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Time period from 1901 through 2000 of the common era.
Time period from 1801 through 1900 of the common era.
Occasions to commemorate an event or occasions designated for a specific purpose.
Time period from 2001 through 2100 of the common era.
A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Substances that are energetically unstable and can produce a sudden expansion of the material, called an explosion, which is accompanied by heat, pressure and noise. Other things which have been described as explosive that are not included here are explosive action of laser heating, human performance, sudden epidemiological outbreaks, or fast cell growth.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The interactions between a host and a pathogen, usually resulting in disease.
Parasitic attack or subsistence on the skin by members of the order Phthiraptera, especially on humans by Pediculus humanus of the family Pediculidae. The hair of the head, eyelashes, and pubis is a frequent site of infestation. (From Dorland, 28th ed; Stedman, 26th ed)
A toxin produced by SHIGELLA DYSENTERIAE. It is the prototype of class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS.
Strains of ESCHERICHIA COLI with the ability to produce at least one or more of at least two antigenically distinct, usually bacteriophage-mediated cytotoxins: SHIGA TOXIN 1 and SHIGA TOXIN 2. These bacteria can cause severe disease in humans including bloody DIARRHEA and HEMOLYTIC UREMIC SYNDROME.
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
Infections with bacteria of the species ESCHERICHIA COLI.
A syndrome that is associated with microvascular diseases of the KIDNEY, such as RENAL CORTICAL NECROSIS. It is characterized by hemolytic anemia (ANEMIA, HEMOLYTIC); THROMBOCYTOPENIA; and ACUTE RENAL FAILURE.
Swollen veins in the lower part of the RECTUM or ANUS. Hemorrhoids can be inside the anus (internal), under the skin around the anus (external), or protruding from inside to outside of the anus. People with hemorrhoids may or may not exhibit symptoms which include bleeding, itching, and pain.
A high-molecular-weight polymeric elastomer derived from the milk juice (LATEX) of HEVEA brasiliensis and other trees and plants. It is a substance that can be stretched at room temperature to at least twice its original length and after releasing the stress, retract rapidly, and recover its original dimensions fully.
A cutaneous pouch of skin containing the testicles and spermatic cords.
The act or ceremony of putting a corpse into the ground or a vault, or into the sea; or the inurnment of CREMAINS.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that may be pathogenic for frogs, fish, and mammals, including man. In humans, cellulitis and diarrhea can result from infection with this organism.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs singly, in pairs, or in short chains. Its organisms are found in fresh water and sewage and are pathogenic to humans, frogs, and fish.
A necessary enzyme in the metabolism of galactose. It reversibly catalyzes the conversion of UDPglucose to UDPgalactose. NAD+ is an essential component for enzymatic activity. EC 5.1.3.2.
Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.
The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Systems consisting of two enzymes, a modification methylase and a restriction endonuclease. They are closely related in their specificity and protect the DNA of a given bacterial species. The methylase adds methyl groups to adenine or cytosine residues in the same target sequence that constitutes the restriction enzyme binding site. The methylation renders the target site resistant to restriction, thereby protecting DNA against cleavage.
The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Methylases that are specific for CYTOSINE residues found on DNA.
The collection, preparation, and distribution of news and related commentary and feature materials through such media as pamphlets, newsletters, newspapers, magazines, radio, motion pictures, television, and books. While originally applied to the reportage of current events in printed form, specifically newspapers, with the advent of radio and television the use of the term has broadened to include all printed and electronic communication dealing with current affairs.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
The white of an egg, especially a chicken's egg, used in cooking. It contains albumin. (Random House Unabridged Dictionary, 2d ed)
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Derivatives of PHOSPHATIDYLCHOLINES obtained by their partial hydrolysis which removes one of the fatty acid moieties.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.

Complete genomic sequence of the lytic bacteriophage DT1 of Streptococcus thermophilus. (1/1064)

Streptococcus thermophilus lytic bacteriophage DT1, isolated from a mozzarella whey, was characterized at the microbiological and molecular levels. Phage DT1 had an isometric head of 60 nm and a noncontractile tail of 260 x 8 nm, two major structural proteins of 26 and 32 kDa, and a linear double-stranded DNA genome with cohesive ends at its extremities. The host range of phage DT1 was limited to 5 of the 21 S. thermophilus strains tested. Using S. thermophilus SMQ-301 as a host, phage DT1 had a burst size of 276 +/- 36 and a latent period of 25 min. The genome of phage DT1 contained 34,820 bp with a GC content of 39.1%. Forty-six open reading frames (ORFs) of more than 40 codons were found and putative functions were assigned to 20 ORFs, mostly in the late region of phage DT1. Comparative genomic analysis of DT1 with the completely sequenced S. thermophilus temperate phage O1205 revealed two large homologous regions interspersed by two heterologous segments. The homologous regions consisted of the early replication genes, the late morphogenesis genes, and the lysis cassette. The divergent segments contained the DNA packaging machinery, the major structural proteins, and remnants of a lysogeny module.  (+info)

Purification and properties of bacteriolytic enzymes from Bacillus licheniformis YS-1005 against Streptococcus mutans. (2/1064)

To find a novel lytic enzyme against cariogenic Streptococci, strains showing strong lytic activity have been screened from soil using Streptococcus mutans. A strain identified as Bacillus licheniformis secreted two kinds of lytic enzymes, which were purified by methanol precipitation, CM-cellulose chromatography, gel filtration, and hydroxyapatite chromatography. The molecular weights of these two enzymes, L27 and L45, were 27,000 and 45,000, respectively. Optimum pH and temperature of both enzymes for lytic activity were pH 8 and 37 degrees C. L27 and L45 digest the peptide linkage between L-Ala and D-Glu in peptidoglycan of Streptococcus mutans. The lytic activity was highly specific for Streptococcus mutans, suggesting their potential use as a dental care product.  (+info)

Structural and functional analysis of pCI65st, a 6.5 kb plasmid from Streptococcus thermophilus NDI-6. (3/1064)

The 6.5 kb cryptic plasmid pCI65st from Streptococcus thermophilus NDI-6, a strain isolated from the Indian fermented milk dahi, was subcloned and sequenced. Five putative ORFs were identified. ORF1 could encode a 315 aa polypeptide almost identical to the RepA protein of previously sequenced S. thermophilus plasmids, indicating that pCI65st is one of the pC194 group of small gram-positive rolling-circle plasmids. ORFs 2 and 4 were virtually identical and could specify proteins of approximately 150 aa with significant similarity to the small heat-shock proteins described from a variety of gram-positive bacteria. ORF3 could encode a 415 aa protein similar to enolase, an enzyme involved in glycolysis and gluconeogenesis. ORF5 could encode a 412 aa protein which had high similarity to the HsdS (specificity) proteins of type I restriction-modification systems. Variants of strain NDI-6 which lacked pCI65st were readily isolated after subculture of the parent strain at 32 degrees C. The plasmid-bearing parent culture was significantly more resistant to a temperature shift from 42 degrees C to 62 degrees C than its plasmid-free variant and expressed proteins which corresponded with the predicted translation products from ORF2 and ORF4. In addition, plasmid-free mutants were lysed in broth by bacteriophages to which the parent culture was resistant.  (+info)

Partial characterization of a major autolysin from Mycobacterium phlei. (4/1064)

Autolytic enzyme profiles of fast- and slow-growing mycobacteria were examined using SDS-PAGE zymography with incorporated mycobacterial peptidoglycan sacculi as substrate. Each species tested (Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium aurum, Mycobacterium fortuitum and Mycobacterium kansasii) appeared to produce a different set of enzymes on the basis of differing number and molecular masses. A major autolysin from M. phlei was purified to apparent homogeneity by DEAE-cellulose chromatography, preparative gel electrophoresis and Mono Q FPLC. This enzyme had an estimated molecular mass of 38 kDa, an isoelectric point of 5.5 and a pH optimum of pH 7.5. Digestion of purified peptidoglycan by the enzyme resulted in the appearance of reducing sugars, suggesting that the 38 kDa autolysin is a beta-glycosidase. Partial internal amino acid sequence of the autolysin was determined and should facilitate identification, cloning and overexpression of the encoding gene.  (+info)

Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase expressed by the alternative vegetative sigma factor, sigmaD. (5/1064)

A gamma-D-glutamate-meso-diaminopimelate muropeptidase was detected in the vegetative growth phase of Bacillus subtilis 168. It is encoded by the monocistronic lytF operon expressed by the alternative vegetative sigma factor, sigmaD. Sequence analysis of LytF revealed two domains, an organization common to exoproteins of B. subtilis as well as to those from other organisms. The N-terminal domain contains a fivefold-repeated motif attributed to cell wall binding, whilst the C-terminal domain is probably endowed with the catalytic activity. Overexpression of LytF allowed its purification and biochemical characterization. Inactivation of lytF led to the loss of the cell-wall-bound protein 49' (CWBP49') and of the corresponding lytic activity as revealed by renaturation gel assay. Native cell walls prepared from the multiple lytC lytD lytE lytF-deficient mutant did not exhibit any autolysis, whereas walls prepared from a strain endowed with LytF but not with the other three enzymes underwent a slight lysis. Analysis of degradation products of cell wall devoid of teichoic-acid-bound O-esterified D-alanine unambiguously confirmed that LytF cuts the gamma-D-glutamate-mesodiaminopimelate bond.  (+info)

A theoretical and empirical investigation of the invasion dynamics of colicinogeny. (6/1064)

A mathematical model describing the dynamics of a colicinogenic and a colicin-sensitive population propagated under serial transfer culture conditions was formulated. In addition, a series of in vitro invasion experiments using six representatives of the E colicin group was undertaken, together with the estimation of the growth rates and colicinogenic characteristics of the strains. Growth rates among the strains varied by up to 44%. There were 14-fold differences among strains in their lysis rates and there were up to 10-fold differences in the amount of colicin produced per lysed cell. The in vitro serial transfer invasion experiments revealed that regardless of initial frequency all colicinogenic strains succeeded in displacing the sensitive cell populations. The amount of time required for the colicin-sensitive cell population to be displaced declined as the initial frequency of the colicinogenic population increased and strains producing higher titres of colicin tended to displace the sensitive strain more rapidly. Overall, the observed dynamics of the invasion of colicinogenic strains was adequately described by the theoretical model. However, despite there being substantial differences among the strains in their growth rates and colicinogenic characteristics there were relatively few differences, observed or predicted, in the invasion dynamics of the six colicinogenic strains. These results suggest that the characteristics of different colicinogenic strains cannot be used to explain the extensive variation in the relative abundance of different colicins in natural populations of bacteria.  (+info)

The C-terminal sequence of the lambda holin constitutes a cytoplasmic regulatory domain. (7/1064)

The C-terminal domains of holins are highly hydrophilic and contain clusters of consecutive basic and acidic residues, with the overall net charge predicted to be positive. The C-terminal domain of lambda S was found to be cytoplasmic, as defined by protease accessibility in spheroplasts and inverted membrane vesicles. C-terminal nonsense mutations were constructed in S and found to be lysis proficient, as long as at least one basic residue is retained at the C terminus. In general, the normal intrinsic scheduling of S function is deranged, resulting in early lysis. However, the capacity of each truncated lytic allele for inhibition by the S107 inhibitor product of S is retained. The K97am allele, when incorporated into the phage context, confers a plaque-forming defect because its early lysis significantly reduces the burst size. Finally, a C-terminal frameshift mutation was isolated as a suppressor of the even more severe early lysis defect of the mutant SA52G, which causes lysis at or before the time when the first phage particle is assembled in the cell. This mutation scrambles the C-terminal sequence of S, resulting in a predicted net charge increase of +4, and retards lysis by about 30 min, thus permitting a viable quantity of progeny to accumulate. Thus, the C-terminal domain is not involved in the formation of the lethal membrane lesion nor in the "dual-start" regulation conserved in lambdoid holins. Instead, the C-terminal sequence defines a cytoplasmic regulatory domain which affects the timing of lysis. Comparison of the C-terminal sequences of within holin families suggests that these domains have little or no structure but act as reservoirs of charged residues that interact with the membrane to effect proper lysis timing.  (+info)

Characterization of a chromosomally encoded glycylglycine endopeptidase of Staphylococcus aureus. (8/1064)

The authors previously reported the cloning of a lytic-enzyme-encoding gene, lytM, from an autolysis-defective mutant of Staphylococcus aureus. In the present work, the lytM gene was overexpressed in Escherichia coli and the product was purified to homogeneity by affinity chromatography and HPLC. Biochemical analysis of LytM-cleaved peptidoglycan fragments indicated that LytM is a glycylglycine endopeptidase. Immunoelectron microscopic studies with anti-LytM rabbit IgG showed that LytM is expressed during the early exponential phase and is overexpressed in an autolysis-defective mutant compared with the parent strain. Also, a uniform distribution of gold particles on the surface of actively growing bacterial cells indicates that LytM plays a role in cell growth. Northern blot analyses of lytM expression in two global regulatory mutants, agr and sar, showed that expression of lytM is increased about twofold in these mutants as compared with the parents. Protein homology searches revealed that LytM could be a member of the zinc protease family, as it contained a homologous 38-amino-acid motif, Tyr-X-His-X11-Val-X12/20-Gly-X5-6-His. Atomic absorption spectrometric analysis of LytM revealed the presence of 0.9 mol zinc (mol LytM)(-1).  (+info)

That communication can occur between virus-infected cells has been appreciated for nearly as long as has virus molecular biology. The original virus communication process specifically was that seen with T-even bacteriophages-phages T2, T4, and T6-resulting in what was labeled as a lysis inhibition. Another proposed virus communication phenomenon, also seen with T-even phages, can be described as a phage-adsorption-induced synchronized lysis-inhibition collapse. Both are mediated by virions that were released from earlier-lysing, phage-infected bacteria. Each may represent ecological responses, in terms of phage lysis timing, to high local densities of phage-infected bacteria, but for lysis inhibition also to locally reduced densities of phage-uninfected bacteria. With lysis inhibition, the outcome is a temporary avoidance of lysis, i.e., a lysis delay, resulting in increased numbers of virions (greater burst size). Synchronized lysis-inhibition collapse, by contrast, is an accelerated lysis which is
Synonyms for Cell lysis in Free Thesaurus. Antonyms for Cell lysis. 19 words related to lysis: convalescence, recuperation, recovery, biochemistry, autolysis, self-digestion, bacteriolysis, cytolysis, dissolution, disintegration. What are synonyms for Cell lysis?
TY - JOUR. T1 - Lactic acid permeabilizes gram-negative bacteria by disrupting the outer membrane. AU - Alakomi, Hanna-Leena. AU - Skyttä, Eija. AU - Saarela, Maria. AU - Mattila-Sandholm, Tiina. AU - Latva-Kala, Kyösti. AU - Helander, Ilkka. PY - 2000. Y1 - 2000. N2 - The effect of lactic acid on the outer membrane permeability ofEscherichia coli O157:H7, Pseudomonas aeruginosa, and Salmonella enterica serovar Typhimurium was studied utilizing a fluorescent-probe uptake assay and sensitization to bacteriolysis. For control purposes, similar assays were performed with EDTA (a permeabilizer acting by chelation) and with hydrochloric acid, the latter at pH values corresponding to those yielded by lactic acid, and also in the presence of KCN. Already 5 mM (pH 4.0) lactic acid caused prominent permeabilization in each species, the effect in the fluorescence assay being stronger than that of EDTA or HCl. Similar results were obtained in the presence of KCN, except for P. aeruginosa, for which an ...
en] amino acids analysis ; isolation & purification ; glycoproteins analysis ; glycine analysis ; escherichia coli ; cell wall analysis ; bacteriolysis ; bacteria analysis ; amino sugars ...
The complement system (Chap. 308) consists of a group of serum proteins functioning as a cooperative, self-regulating cascade of enzymes that adhere to- and in some cases disrupt-the surface of invading organisms. Some of these surface-adherent proteins (e.g., C3b) can then act as opsonins for destruction of microbes by phagocytes. The later, terminal components (C7, C8, and C9) can directly kill some bacterial invaders (notably, many of the neisseriae) by forming a membrane attack complex and disrupting the integrity of the bacterial membrane, thus causing bacteriolysis. ...
Complex cookies of book Viagem Fluvial do Tietê ao monitoring. A innovative DNA certificate for the IoT of social functions in everyone. bacteriolysis of experience attention by adaptive antibiotics.
Since most RNA-binding proteins fulfil their function in the context of RNA-protein complexes, knowledge of RNAs associated with specific RBPs is essential to elucidate their functions. In order to identify these transcripts, new methods must be developed or existing successful protocols for the identification of protein-protein interactions must be adapted. Although several recent publications have identified RNA partners from RNP-complexes [9, 16], there are so far no reports on the quality of the RNAs purified from these complexes. Here we demonstrate that the method used for cell lysis of yeast cells is of great importance for isolation of intact complexes. Whereas standard lysis methods like disruption by French Press or glass bead mill lead to massive RNA degradation (Figures 1, 2, 3), grinding yeast cells at ultra-low temperatures leaves cellular RNA intact (Figures 4, 5).. The major difference between the lysis methods compared in this study is the timing between addition of the RNase ...
TY - JOUR. T1 - Identification of a novel inhibition site in translocase MraY based upon the site of interaction with lysis protein e from bacteriophage φx174. AU - Rodolis, Maria T.. AU - Mihalyi, Agnes. AU - OReilly, Amy. AU - Slikas, Justinas. AU - Roper, David I.. AU - Hancock, Robert. AU - Bugg, Timothy D.H.. PY - 2014/6/16. Y1 - 2014/6/16. N2 - Translocase MraY is the site of action of lysis protein E from bacteriophage φX174. Previous genetic studies have shown that mutation F288L in transmembrane helix 9 of E. coli MraY confers resistance to protein E. Construction of a helical wheel model for transmembrane helix 9 of MraY and the transmembrane domain of protein E enabled the identification of an Arg-Trp-x-x-Trp (RWxxW) motif in protein E that might interact with Phe288 of MraY and the neighbouring Glu287. This motif is also found in a number of cationic antimicrobial peptide sequences. Synthetic dipeptides and pentapeptides based on the RWxxW consensus sequence showed inhibition of ...
Perform an efficient extraction of soluble proteins from bacterial cells with SoluLyse from Genlantis, a high-yielding and optimized bacterial lysis reagent.
Principal Investigator:EZURA Yoshio, Project Period (FY):1992 - 1994, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:Fisheries chemistry
The Casework Direct System protocol entails a simple and rapid lysis of the sample, requiring no tube-to-tube transfers or DNA binding steps, producing a lysate compatible with RT-PCR quantification and PowerPlex STR amplification systems.
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We used the Apoptosis Cassette by Brown 08 iGEM team (K124017). The bacteriophage cassette includes S105 protein (Holin), R protein (Endolysin), and Rz protein, all of which act in combination to lyse the bacteria. Holin is a small membrane protein that produces holes in the membrane. R is an endolysin that digests and cleaves the cell wall. Rz protein, a periplasmic protein, through reasons that are not yet totally clear, carries out the final step of host lysis. ...
We propose four stochastic models. 1. First model is that the effect of endolysin for cell lysis is limited though the concentration of endolysin increases. We consider that when much endolysin is expressed, the size of holes made by holin restricts the transition rate of endolysin from cytosol to periplasm, where endolysin degrades peptidoglycan and makes cell lysis. 2. Second model is that the expression of holin and endolysin is not enough to kill every cell entirely. In this model, we assume that some cells are weak to the cell lysis and other cells are tough to that. If this is true, some proportional cells survive and the other proportional cells are died constantly (Fig.2). 3. Third model is that quantity of expressed holin and endolysin is different from cells respectively. We assume that every cell has the threshold of lysis. In any concentration of IPTG, some proportional cells survive and the other cells are killed because of the difference of expression. (Fig.3) ...
Holin je u vodi rastvoran esencijalni nutrijent.[4][5][6][7] On se obično svrstava u B-kompleks vitamina. Holin se normalno javlja u obliku raznih kvaternarnih amonijum soli koje sadrže N,N,N-trimetiletanolamonijum katjon.. Katjon holina se javlja kao čeona grupa fosfatidilholina i sfingomijelina, dve klase fosfolipida koje su široko rasprostranjene u ćelijskim membranama. Holin je prekursorni molekul za neurotransmiter acetilholin koji ima veliki broj funkcija, kao što su memorija i kontrola mišića.. Holin se mora uneti putem hrane da bi telo ostalo zdravo.[8] On se koristi u sintezi gradivnih komponenti ćelijskih membrana.[9]. ...
A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
Holin se v organizmu presnovi zlasti do trimetilamina, ki ima vonj po ribah. Velika količina zaužitega holina lahko zato povzroči neprijeten telesni vonj. Pri določeni genetski motnji, trimetilaminuriji, bolniki niso zmožni nadalje razgraditi trimetilamina in posledica je močan telesni vonj po ribah. Pri ublažitvi telesnega vonja pomaga dieta, ki vsebuje čim manj holina.. ...
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Immunization of rabbits with concentrated Brucella abortus antigens for 10 weeks led to nearly total loss of serum bactericidal activity for this organism. β and γ globulin fractions of hyperimmune serum obtained by starch block electrophoresis had bactericidal activity. Sephadex G-200 gel filtration of the β globulin fraction obtained by starch block electrophoresis yielded a fraction consisting of IgM and another containing IgG and IgA; both had bacteriolytic activity. The latter subfraction was separated by column chromatography on DEAE-Sephadex into IgG and IgA (probably contaminated by 15% to 20% IgG). Bacteriolytic tests indicated that IgA had a prozone of inhibition of bacteriolysis, whereas IgM and IgG did not. Addition of excess IgA inhibited bacteriolysis produced by IgG and IgM. Bactericidal kinetics disclosed little kill by whole hyperimmune serum. Hyperimmune IgM and IgG killed Br, abortus rapidly but IgA fractions gave a lag of more than 3 min before any kill was measured. ...
The scientific literature shows a renewed interest in phage therapy. Part of this interest stems from a series of spectacular experiments conducted with phage lysins (31, 37, 44), another part comes from a number of recent animal experiments with viable phage particles as antibacterial agents (8, 11, 14, 28, 30, 32, 49, 54), and a further part derives from historical reviews of the Soviet experience with phage therapy (4, 10, 12, 18, 48, 49, 50). It is currently difficult to critically assess the potential of the clinical trials conducted in the former Soviet Union. The trials were mainly published in Russian, most of the trials were uncontrolled, and the therapeutic phages were not described in published reports. With the present study, we wanted to address some of the basic preclinical problems of phage therapy in the context of E. coli diarrhea. We have chosen this example because a substantial body of knowledge has been accumulated on E. coli and its phages; the pathogenic target bacteria ...
Yersinia pestis, the agent of plague, requires the Ail (attachment invasion locus) outer membrane protein to survive in the blood and tissues of its mammalian hosts. Ail is important for both attachment to host cells and for resistance to complement-dependent bacteriolysis. Previous studies have sho …
Buffer B2 is used in combination with Buffer B1, lysozyme or lysostaphin and Proteinase K for efficient lysis of bacteria prior to DNA purification using QIAGEN Genomic-tips. Genomic-tips are gravity-flow, anion-exchange columns. Please note this buffer is not recommended for any purification procedures using QIAGENs silica-membrane-based spin columns. Buffer B2 (Bacterial Lysis Buffer 2) consists of 3 M guanidine hydrochloride, 20% Tween 20.. How to prepare Buffer B2: Dissolve 286.59 g guanidine hydrochloride in 700 ml distilled water. Add 200 ml 100% Tween 20. Adjust the volume to 1 liter with distilled water. pH does not need to be adjusted. ...
Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[(Man)5(GlcNAc)2]-Asn structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact. Cleaves the peptidoglycan connecting the daughter cells at the end of the cell division cycle, resulting in the separation of the two newly divided cells. Acts as an autolysin in penicillin-induced lysis (By similarity).
Founded in 2001, ACMA Clinic provides professional and comprehensive health care of holistic and natural medicine.. ACMA patients come from 50 states in U.S. and countries in North America, South America, Asia, Europe, etc. Most ACMA patients first started treatments with their family doctors, specialists, local hospitals, and leading hospitals in U.S. or other countries. When those treatments could not help them, they started ACMA treatment. As a result, when they came to ACMA, they had been suffering from various severe diseases and illnesses for years, and many of them had been regarded as incurable.. However, after ACMA treatments, most patients conditions start improving for the first time in their disease history. Patients are happy to see they are recovering and returning back to normal lives.. ...
Preparation of nucleic acid samples prior to amplification and detection of specific targets is arguably the most challenging step of molecular diagnostics. ERBA Molecular has innovative fully integrated sample collection and extraction technologies and a number of products that enable the simultaneous collection, rapid lysis preservation and isolation of amplifiable quality nucleic acids from bacterial, viral and human sample types.. The nucleic acid isolated is of high quality and ideal for molecular diagnostic assays and proven as a rapid NGS front-end solution. ...
A coordinated action of murein synthetases and murein hydrolases is assumed to be essential for proper growth of the bacterial cell. A disturbance of this balance, for example by blocking parts of...
Does anyone know the reagent in BioRads instagene matrix which causes lyses of bacterial cells? Troy Barton University of New Brunswick L14Y at UNB.CA ...
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If you follow the sonication protocol below for cell lysis you should achieve efficient lysis of your cells for your required application. Tips Included.
A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.. ...
Shop Germination-specific N-acetylmuramoyl-L-alanine amidase ELISA Kit, Recombinant Protein and Germination-specific N-acetylmuramoyl-L-alanine amidase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Lysostaphin is an antimicrobial agent belonging to a major class of antimicrobial peptides and proteins known as the bacteriocins. Bacteriocins are bacterial antimicrobial peptides which generally exhibit bactericidal activity against other bacteria. Bacteriocin production is a self-protection mechanism that helps the microorganisms to survive in their natural habitats. Bacteriocins are currently distributed into three main classes. Staphylococcins are bacteriocins produced by staphylococci, which are Gram-positive bacteria of medical and veterinary importance. Lysostaphin is the only class III staphylococcin described so far. It exhibits a high degree of antistaphylococcal bacteriolytic activity, being inactive against bacteria of all other genera. Infections caused by staphylococci continue to be a problem worldwide not only in healthcare environments but also in the community, requiring effective measures for controlling their spread. Since lysostaphin kills human and animal staphylococcal pathogens,
Caspase-11 directly detects LPS within the host cell cytosol (6). To explain how LPS gains access to the host cell cytosol, four distinct LPS delivery pathways were proposed: (i) some intracellular Gram-negative bacteria escape vacuoles to enter the host cell cytosol, where they release LPS (30); (ii) host GBPs execute membranolytic activities to extrude intracellular Gram-negative bacteria from PVs and extract LPS through bacteriolysis (10, 19-21); (iii) endocytosed bacterial OMVs release LPS into the host cell cytosol potentially through fusion with or transport across endosomal membranes (15, 31); and (iv) circulating free LPS (in the form of aggregates or bound to LPS-binding proteins) is consumed in vivo by an undefined cell population able to present LPS for caspase-11-mediated recognition (4, 5). Here, we present evidence that GBPs play previously unknown roles in the latter two pathways.. GBPs assist caspase-11 activation in response to infections with Gram-negative bacteria (10-12). It ...
A bacterial strain designated SBKo001T was isolated from a forest soil sample from Mt Makiling in Laguna, Philippines. It shows the general characteristics associated with myxobacteria, such as swarming of Gram-negative, rod-shaped vegetative cells, fruiting body formation and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic and also exhibits resistance to various antibiotics. Major fatty acids are iso-C15 : 0, C17 : 1 2-OH and C20 : 4 (arachidonic acid). The G+C content of the genomic DNA is 69.2 mol%. A reference strain, NOSO-1 (=DSM 53757), isolated from the Etosha Basin in Namibia, shares nearly the same characteristics with SBKo001T. The identical 16S rRNA gene sequences of the two strains show 94 % identity to strains of the cellulose-degrading Byssovorax and Sorangium species. Phylogenetic analysis reveals a novel branch diverging from the Polyangiaceae, Sorangiineae, Myxococcales. Their uniqueness in morphological growth stages, unusual fatty acid profile
1) Two extracellular staphylolytic enzymes have been Isolated and purified from submerged cultures of Streptomyces griseus. 2) The enzymes were electrophoretically homogeneous at several pH values. A single peak was obtained for each protein on ultracentrifugation. Based on gel-filtration molecular weights of 11,800 and 13,000 were determined for enzyme 1 and enzyme 2 respectively. 3) The ionic strength and pH optima for lytic activity of the two enzymes against staphylococcal cells and their isolated cell walls have been detenained. The effects of several cations and certain known enzyme inhibitors have been tested on lytic action of the two enzymes against cell walls. 4) Both enzymes were N-acetylhexosaminidases. Examination of the acid hydrolysis products of fully digested cell walls treated with naBH4 demonstrated that both enzymes were muramidases. 5) Both enzymes caused lysis to varying extents, of the cell walls of a range of gram positive organisms. However, E. coli, the only gram ...
Shop Peptidoglycan hydrolase ELISA Kit, Recombinant Protein and Peptidoglycan hydrolase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is certainly reported for 45 and strains isolated in Egypt. a assortment of and isolates from Egypt for their differential sensitivity to boiling in water as measured by the release of PCR-detectable DNA. A total of 45 randomly selected and strains isolated from field studies in Upper Egypt of pediatric diarrhea were used. AR-C155858 These strains were confirmed biochemically and morphologically, then were produced on Skirrows medium (5) at 42C for 48 h under an atmosphere of 10% CO2, 5% O2, and 85% N2. Two methods were used for the preparation of DNA for PCR. The first was the boiling method (9), in which a loopful of bacteria was washed with 1 ml of distilled water (dH2O) and resuspended in 200 l of dH2O. The suspension was boiled for 10 min, placed on ice for 5 min, and then centrifuged for 5 min in a microcentrifuge at 10,000 to extract DNA. The second method (8) involved bacterial lysis by treatment ...
The culturability of several actinobacteria is controlled by resuscitation-promoting factors (Rpfs). These are proteins containing a c. 70-residue domain that adopts a lysozyme-like fold. The invariant catalytic glutamate residue found in lysozyme and various bacterial lytic transglycosylases is also conserved in the Rpf proteins. Rpf from Micrococcus luteus, the founder member of this protein family, is indeed a muralytic enzyme, as revealed by its activity in zymograms containing M. luteus cell walls and its ability to (i) cause lysis of Escherichia coli when expressed and secreted into the periplasm; (ii) release fluorescent material from fluorescamine-labelled cell walls of M. luteus; and (iii) hydrolyse the artificial lysozyme substrate, 4-methylumbelliferyl-β-d-N,N,N-triacetylchitotrioside. Rpf activity was reduced but not completely abolished when the invariant glutamate residue was altered. Moreover, none of the other acidic residues in the Rpf domain was absolutely required for ...
The extraction and purification of genetic material from cells is often the first and most crucial step in life sciences research. Gram-positive bacteria, which contain thick cell walls, pose a challenge for efficient and effective nucleic acid extraction in this regard.. Dr. Vincent A. Fischetti and his research group have developed an effective enzyme-based nucleic acid extraction system. A group of enzymes isolated from viruses that infect specific bacteria (bacteriophages) was discovered as a potential antibacterial agent, and their mode of action is to compromise the integrity of the bacterial cell wall or membrane resulting in rapid lysis of the bacterial cell. Once the cell wall is removed, it is fairly straightforward to obtain the DNA or RNA for subsequent experiments, such as PCR, cloning, sequencing, and proteomics. The use of these enzymes as reagents for nucleic acid extraction shortens the time and increases the nucleic acid yield, which is greatly advantageous.. Several lysins ...
All known pneumococcal phages contain a cell wall lytic system consisting of a holin that permeabilizes the cell membrane, and either an N-acetylmuramoyl-L-alanine amidase (amidase) or a lysozyme, capable of digesting the pneumococcal cell wall (8 ). ...
Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.. ...
2001b, 2007, 2008). Mutation in the lytM gene was subsequently transduced into the S. aureus lyt− strain (Mani et al., 1993; Ramadurai & Jayaswal, 1997) to potentially create an autolysin-free lyt−:lytM double mutant. For genetic complementation of the lytM mutant, an approximately 2.2-kb DNA fragment was PCR amplified using primers P5 and P6 and S. aureus SH1000 genomic DNA as template. This amplicon represents a fragment starting 890 nt upstream and ending 364 nt downstream of the lytM gene that was cloned into the BamHI and HindIII Screening Library manufacturer sites. of shuttle plasmid pCU1 (Augustin et al., 1992) and subsequently transferred to a lytM mutant of S. aureus SH1000. Mid-exponential-phase cultures (OD600 nm=0.6) were diluted 50-fold in a nephelo culture flask (Wheaton) containing 50 mL fresh TSB with a flask-to-medium volume ratio of 6 : 1 and growth was followed by measurement of OD600 nm spectrophotometrically. In another experiment, cultures pregrown to an OD600 nm=0.5 ...
Words starting with A (page 114): autokinesis, autokinetic, autolatries, autolatry, autoload, autoloader, autoloading, autologies, autologous, autology, autolysate, autolyse, autolysed, autolyses, autolysin, autolysing, autolysins, autolysis, autolytic, autolyzate...
Is there an optimal incubation time for cell lysis? - posted in General Lab Techniques: I have been looking through many cell lysis protocols over the last months and I noticed a huge variety of incubations times although most protocols had the same enzymes or detergents. Granted, some were for pure grown cultures and others for environmental samples, but I was wondering why the incubation times are so different (with such a wide range). For example, there were protocols for enzymatic lysi...
A summary of the key influences, organisations and principles that guide the ACMAs approach to spectrum planning. Click here to learn more.
Edna talks about the problems of tension and also relaxation, and how to play with the support of the hand and forearm. http://youtu.be/yu6sPvPg1CI
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page. ...
Defects in the formation of the septum and gradually autolysis of cells occur when the dapdependent mutant ofEscherichia coli is grown in a medium with 4-hydroxy-diaminopimelic... ...
I f you havent heard of Dame Edna Everage well, darling, it just means youre frightfully unaware of anything but your drab, uninteresting life.Because Dame Edna, though shes a man deliciously
Acta Sci. Pol. Zootechnica 14(1) 2015, ISSN ISSN (online) EFFECTIVE BACTERIOLYSIS OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O157: H7 CAUSED BY SPECIFIC BACTERIOPHAGE
Staphylococcus aureus; pan ID: SAUPAN004467000; products: autolysin, N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoprotein endo-beta-N-acetylglucosamidase, beta-N-acetylglucosaminidase, putative, exported protein, family 4 N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoendo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-glucosaminidase, peptidoglycan endo-beta-N-acetylglucosaminidase
Krogh, S, Jorgensen, ST, Devine, KM, Lysis genes of the Bacillus subtilis defective prophage PBSX, JOURNAL OF BACTERIOLOGY, 180, 8, 1998, 2110-2117 ...
Staphylococcus aureus subsp. aureus bacteriophage 52 ATCC ® 27692-B1™ Designation: CDC 52 TypeStrain=False Application:
Bacteriophages escaping from a dying bacterial cell (Streptococcus sp.), coloured scanning electron micrograph (SEM). This bacteriophage was discovered in freshwater near a sewage outlet. A bacteriophage, also known as a phage, is a virus (virion) that infects a bacterium. It consists of a head (capsid), containing the genetic material (either RNA or DNA) and usually a tail and tail fibres (not seen), which the phage uses to attach to a specific receptor sites on the bacterium. This specific binding means that a bacteriophage can only infect certain bacteria bearing specific receptors. Once attached to the cell surface genetic material is injected into the bacterium, taking over the bacteriums own cellular machinery and forcing it to produce more copies of the bacteriophage. When sufficient numbers have been produced the phages escape from the bacterium by cellular lysis, killing the bacterium in the process. Magnification: x21,335 when shortest - Stock Image C032/0258
A disinfectant can be a chemical professional that is used to reduce the quantity of practical microbes on drug types of surface with an suitable degree. Disinfectants have a variety of properties which include range of exercise, function of action, and efficiency. Some are bacteriostatic, the place that the capacity with the microbe population to breed is halted. In such a case, the disinfectant may cause picky and reversible improvements to microbial tissues by getting together with nucleic acids and inhibiting digestive support enzymes, or permeating in to the mobile wall surface. Once the disinfectant is removed from experience of microbial cellular material, the surviving microbe human population can potentially develop. Other disinfectants are bactericidal for the reason that they eradicate bacterial cells and lead to irreversible damage by way of distinct systems offering architectural damage to the cellular, cellular lysis, and autolysis, leading to loss or coagulation of cytoplasm. The ...
A composition for treatment of bacterial infections of the eye is disclosed which comprises a lytic enzyme composition specific for the infecting bacteria, and a carrier for delivering said lytic enzyme. The carrier for delivering at least one lytic enzyme to the eye may be but is not limited to the use of an isotonic solution.
I know its growth temperature is around 26 degrees celsius but it is a really resistant bacteria. I was wondering if anyone knew if it could survive in higher (30-40 degrease) or lower (0-5) temperatures ...
The objective of this proposal is to understand how the pigment causes host cell lysis and induces an immune response and to also define how pigment mediated activation of host cells affects Group B streptococcal (GBS) infection colonization and infection-associated preterm births.. ...
The objective of this proposal is to understand how the pigment causes host cell lysis and induces an immune response and to also define how pigment mediated activation of host cells affects Group B streptococcal (GBS) infection colonization and infection-associated preterm births.. ...
Detection of soluble and insoluble proteins: Drummond:Solubility Buffers for lysis: Drummond:Lysis Protein slot-blot: Drummond:Protein Slot Blot Protocol Ubiquitin western: Drummond:Ubiquitin Western Test page for CSS columns: Drummond:Columns ...
Our Microfluidizer cell disruption equipment allow for effective, efficient cell lysis in laboratory and production applications. Learn more -- request details.
Let your inbox help you discover our best projects, classes, and contests. Instructables will help you learn how to make anything! ...
Lysis buffer preparation - posted in General Lab Techniques: Hi! Ive to prepare 50ml of (Tris0.1M pH8/EDTA 0.1M/ NaCl 0.15M/1%SDS/2%Triton 100x) and I would like to know which is the correct order to add the reactives and prepare it. Could you help me?
Kellenberger, G.; Kellenberger, E. (1952-01-01). "[Bacteriolysis of a strain of bacillus cereus; evidence in electronic ...
CHS is a disease causing impaired bacteriolysis due to failure of phagolysosome formation. As a result of disordered ...
He called this bacteriolysis and it became known as the Pfeiffer Phenomenon, or Isayev-Pfeiffer phenomenon. Working with Robert ... particularly for the phenomenon of bacteriolysis. In 1894 he found that live cholera bacteria could be injected without ill ...
... bacteriolysis). To combat pathogens that replicate outside cells, antibodies bind to pathogens to link them together, causing ...
... aeruginosa microbes were shown to fuse with outer membrane of other gram negative microbes causing their bacteriolysis; these ...
Bacteriolysis in hypoxic tumor parts can be combined with chemotherapy and/or radiation that are more effective in ...
... such as antibody-initiated complement-dependent bacteriolysis, opsonoization, phagocytosis and killing, as occurs for some ...
Bacteriolysis (Richard Pfeiffer) 1896 - An antibacterial, heat-labile serum component (complement) is described (Jules Bordet) ...
... monocytogenes by causing bacteriolysis within the host cytosol. Current research in the Liang Tong's lab focuses on enzymes ...
a direct effect upon a pathogen, such as antibody-initiated complement-dependent bacteriolysis, opsonoization, phagocytosis and ...
... but do not seem to cause bacteriolysis. This study shows that B. pumilus also lyses living cells of A. citreus in co-culture ...
While the bacteriolysis range of L. innocua strains was 48% to 76% after 48 h of incubation, L. welshimeri isolates exhibited ... Bacteriolysis was measured at 650 nm by spectrophotometer. For this purpose, late exponential phase cells were transferred into ... Several authors have reported extracts of this plant to effect rapid bacteriolysis and inhibit growth of a wide range of Gram- ... On the one hand, bacteriolysis may be associated with aggravation of endotoxemia. At the same time, together with Cd-induced ...
Definition of Bacteriolysis and synonyms of Bacteriolysis are presented by online Websters Dictionary. Includes dictionary ... browser, morphological search by meaning of Bacteriolysis, thesaurus, related words, and dictionary browser. Provides ... Bacteriolysis Definition of Bacteriolysis. Bac`te`ri`ol´y`sis. n.. 1.. Chemical decomposition brought about by bacteria without ... Bacteriolysis-. bacteriophage. bacteriophagic. Bacterioscopic. Bacterioscopist. Bacterioscopy. bacteriostasis. bacteriostat. ...
Protamine and Polyarginine Bacteriolysis. Similarities in Its Mechanism with Chromatin DNA Picnosis. Antohi, Stefan / Popescu, ...
Bacteriolysis * Base Sequence * Carbohydrate Sequence * Cloning, Molecular * Endopeptidases / chemistry * Endopeptidases / ...
The seminal paper of Jules Bordet which led to a new concept of bacteriolysis was published in 1895 (21). It was his first ... This led Bordet to propose that the alexin involved in bacteriolysis was the same than the alexin involved in hemolysis. He ... For example, he reconstituted the bacteriolysis activity of a heat-treated preventive serum by adding fresh normal serum. But ... Identification of Complement and Its Interaction With Antibodies to Induce Bacteriolysis. Before Bordet published his first ...
complement-dependent bacteriolysis, opsonoization, phagocytosis and killing, as occurs for some bacteria,*neutralization of ...
Zootechnica 14(1) 2015, ISSN ISSN (online) EFFECTIVE BACTERIOLYSIS OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O157: H7 CAUSED BY ... 5 Effective bacteriolysis of Shiga toxin-producing Escherichia coli OD Control Kontrola MOI = 0.1 MOI = 1 MOI = 5 MOI = Time, ... 3 Effective bacteriolysis of Shiga toxin-producing Escherichia coli H7 strains were isolated from pig slurry and characterized ... 7 Effective bacteriolysis of Shiga toxin-producing Escherichia coli terial strains. Both the positive (e.g., biocontrol, phage ...
Bacteriolysis * Cell Division * Cell Wall / enzymology * Cell Wall / metabolism * N-Acetylmuramoyl-L-alanine Amidase / ...
bacteriolysis. The dissolution of bacteria on the animals body, whether inside or outside the body. ...
... leading to bacteriostasis and bacteriolysis (33, 34). On the basis of the sensitivity of galE mutants to galactose, an A. ...
See also bacteriolysis. bac·te·ri·ol·y·sin (bak-tērē-oli-sin) Specific antibody that combines with bacterial cells (i.e., ...
Pfeiffer phenomenon - bacteriolysis.. Pfeiffer syndrome - Synonym(s): type V acrocephalosyndactyly. Want to thank TFD for its ...
Kellenberger, G.; Kellenberger, E. (1952-01-01). "[Bacteriolysis of a strain of bacillus cereus; evidence in electronic ...
Strominger, J. L., and Ghuysen, J.-M., 1967, Mechanisms of enzymatic bacteriolysis, Science 156:213.PubMedCrossRefGoogle ... Glynn, A. A., 1969, The complement lysozyme sequence in immune bacteriolysis, Immunology 16:463.PubMedGoogle Scholar ... Becker, M. E., and Hartsell, S. E., 1955, The synergistic action of lysozyme and trypsin in bacteriolysis, Arch. Biochem. 55: ... Amano, T., Inai, S., Seki, Y., Kashiba, S., Fujikawa, K., and Nishimura, S., 1954, Studies on the immune bacteriolysis. I. ...
Susceptibility of Vibrio cholerae O139 to antibody-dependent, complement-mediated bacteriolysis.. Attridge SR, Qadri F, Albert ...
These late complement components are required for bacteriolysis of meningococci.. An estimated 50-60% of individuals with late ...
Bacteriolysis.The mean OD620 of untreated S. aureus suspensions after 120 min was 96.9% (n = 9; standard error [SE] = 1.3%) of ... Bacteriolysis.Suspensions of S. aureus were prepared as described above. After retrieval of the pretreatment sample, the agents ... In assays for bacterial killing, bacteriolysis, and loss of 260-nm-absorbing material, samples taken 5 min before treatment ...
Study Antibody structure/function (notes/class) flashcards from Hunter Garrett
V. Modification of bacteriolysis by antiinflammatory agents and by cationic and anionic polyelectrolytes.Inflammation 1:57-69. ... The role played by artificial enzyme "cocktails" and tissue enzymes in bacteriolysis.Inflammation 1:45-56.Google Scholar ... Bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances.J ... aureus mutant which is deficient in teichoic acid and the inhibition of bacteriolysis by lipoteichoic acid.Proc. Soc. Exp. Biol ...
6. MECHANISM OF ACTION TRICLOSAN ACT ON CYTOPLASMIC MEMBRANE INDUCE LEAKAGE OF CELLULAR CONSTITUENTS BACTERIOLYSIS ...
Listeria monocytogenes triggers AIM2-mediated pyroptosis upon infrequent bacteriolysis in the macrophage cytosol. Cell Host ... pyroptosis triggered by hypercytotoxic Francisella mutant strains is attributed to increased intracellular bacteriolysis. Cell ...
Anticapsular antibodies promote phagocytosis and bacteriolysis. During meningitis, phagocytosis is the main host defense ...
Addition of excess IgA inhibited bacteriolysis produced by IgG and IgM. Bactericidal kinetics disclosed little kill by whole ... Bacteriolytic tests indicated that IgA had a prozone of inhibition of bacteriolysis, whereas IgM and IgG did not. ...
... bacteriolysis ; bacteria analysis ; amino sugars analysis. ...
Bacteriolysis. *Endopeptidases/chemistry/genetics/isolation & purification. *Gram-Negative Bacteria/drug effects. *Molecular ...
Cytosolic bacteriolysis is thus key to orchestrate inflammasome-mediated innate immune responses. We propose here to ... Cytosolic bacteriolysis is thus key to orchestrate inflammasome-mediated innate immune responses. We propose here to ... We will develop three synergistic approaches: i) the generation of novel tools to monitor cytosolic bacteriolysis ii) ... We will develop three synergistic approaches: i) the generation of novel tools to monitor cytosolic bacteriolysis ii) ...
23594303 - Bacteriolysis by vancomycin-conjugated acryl nanoparticles and morphological component .... Publication Detail: Type ...
Streptococcus pyogenesEndopeptidase O Contributes to Evasion from Complement-mediated Bacteriolysis via Binding to Human ...
... bacteriolysis).[31] ...
  • This bacteriolysis, Bordet discovered, did not occur when the bacteria was injected into a non-immunized guinea pig, but did so when the same animal received the antiserum from an immunized animal. (faqs.org)
  • Moreover,the bacteriolysis did not take place when the bacteria and the antiserum weremixed in a test tube unless fresh antiserum was used. (faqs.org)
  • Some strains express a polysaccharide capsule, a principal virulence factor that protects bacteria from immune defenses, e.g., complement-dependent bacteriolysis. (cdc.gov)
  • Immune protection against invasive meningococcal disease depends on recognition of bacterial surface antigens by antibodies, followed by activation of complement, leading to degradation of the bacteria by bacteriolysis, also named serum bactericidal activity (SBA). (asm.org)
  • The aim of this study was to isolate the bacteria having bacteriolytic activity from coastal area and to elucidate a mode of their bacteriolysis and a role of them in microbial ecology. (nii.ac.jp)
  • Bacteriolytic tests indicated that IgA had a prozone of inhibition of bacteriolysis, whereas IgM and IgG did not. (jimmunol.org)
  • As both proteins are predicted to locate in the outer membrane of Brucella organisms, the ability of anti-VirB antibodies to mediate complement-dependent bacteriolysis of B. canis was assessed in vitro. (worldwidescience.org)
  • Anticapsular antibodies promote phagocytosis and bacteriolysis. (kenyon.edu)
  • In the late 19th century, serum was found to contain a nonspecific heat-labile complementary principle that interacted with antibodies to induce bacteriolysis. (medscape.com)
  • He showed that bacteriolysis results from the combined action of two factors in blood serum: complement, a non-specific heat-labile factor initially called "alexin", and a specific heat-stable or sensitizing factor (the "antibodies") so called because it sensitizes the microbe to the bacteriolytic action of the alexin. (pasteur.fr)
  • and group A streptococci, but induce neither bacteriolysis nor solubilization of a substantial amount of membrane phospholipids. (springer.com)
  • The Bac41 effector consists of the secreted proteins BacL 1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis. (asm.org)
  • Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. (nih.gov)
  • Its antibacterial activity is bactericidal and causes bacteriolysis at MIC. (mims.com)
  • The role played by artificial enzyme "cocktails" and tissue enzymes in bacteriolysis. (springer.com)
  • Lysozyme takes also part in an extensive battery of defense mechanisms in fish, such as bacteriolysis and opsonization of the bacterial wall, and it is present in lymphoid tissues, mucus, plasma, and other body fluids [ 2 ]. (hindawi.com)
  • Serum complement not only displays antimicrobial activities such as bacteriolysis and opsonization, but is also involved in boosting adaptive immune responses to foreign antigens. (harvard.edu)
  • From 1895 to 1896 he investigated the mechanisms of bacterial agglutination and destruction (bacteriolysis) by immune serum and thus underlined the role of humoral immunity in antimicrobial defense processes. (pasteur.fr)
  • While the bacteriolysis range of L. innocua strains was 48% to 76% after 48 h of incubation, L. welshimeri isolates exhibited broader bacteriolytic vari. (worldwidescience.org)
  • Ginsburg I (2002): The role of bacteriolysis in the pathophysiology of inammation, infec- tion and submit-infectious sequelae. (hautes-alpes.net)
  • These data suggest that a complex mechanism of action underlies lytic killing, as exogenous bacteriolysis induced by lytic bacteriocins or beta-lactams requires an intact cell physiology in E. faecalis . (asm.org)
  • Percentage of phagocytosing phagocytes, NBT reduction activity, potential killing activity and plasma bacteriolysis activity were measured before, during and after the temperature changes. (go.jp)
  • citation needed] CHS is a disease causing impaired bacteriolysis due to failure of phagolysosome formation. (wikipedia.org)
  • The cell of E.coli, etc., is transformed with the obtained manifestation vector containing the chemical synthesis gene of miraculin, the transformed cell is cultured in a medium, the cultivation product is centrifuged and the collected cells are subjected to bacteriolysis by ultrasonic treatment, etc. (sumobrain.com)
  • Ultrasonic(US)method and cation resin(CR)method were combined to extract ex- tracellular polymeric substances(EPS)from activated sludge,and to study the efficiency of the dif- ferent combined extract methods.The research results showed that US-CR was the best than three different ways,US postposition processes such as CR-US or US-CR-US might lead more bacteriolysis. (cnki.com.cn)
  • Similar to the other antibiotics clubbed under this category, Amoxicillin (initially called Amoxycillin) exerts bacteriolysis through the same mode of action. (natural-homeremedies.com)