Techniques used in studying bacteria.

Mechanism of citrate metabolism in Lactococcus lactis: resistance against lactate toxicity at low pH. (1/6674)

Measurement of the flux through the citrate fermentation pathway in resting cells of Lactococcus lactis CRL264 grown in a pH-controlled fermentor at different pH values showed that the pathway was constitutively expressed, but its activity was significantly enhanced at low pH. The flux through the citrate-degrading pathway correlated with the magnitude of the membrane potential and pH gradient that were generated when citrate was added to the cells. The citrate degradation rate and proton motive force were significantly higher when glucose was metabolized at the same time, a phenomenon that could be mimicked by the addition of lactate, the end product of glucose metabolism. The results clearly demonstrate that citrate metabolism in L. lactis is a secondary proton motive force-generating pathway. Although the proton motive force generated by citrate in cells grown at low pH was of the same magnitude as that generated by glucose fermentation, citrate metabolism did not affect the growth rate of L. lactis in rich media. However, inhibition of growth by lactate was relieved when citrate also was present in the growth medium. Citrate did not relieve the inhibition by other weak acids, suggesting a specific role of the citrate transporter CitP in the relief of inhibition. The mechanism of citrate metabolism presented here provides an explanation for the resistance to lactate toxicity. It is suggested that the citrate metabolic pathway is induced under the acidic conditions of the late exponential growth phase to make the cells (more) resistant to the inhibitory effects of the fermentation product, lactate, that accumulates under these conditions.  (+info)

Direct selection for mutators in Escherichia coli. (2/6674)

We have constructed strains that allow a direct selection for mutators of Escherichia coli on a single plate medium. The plate selection is based on using two different markers whose reversion is enhanced by a given mutator. Plates containing limiting amounts of each respective nutrient allow the growth of ghost colonies or microcolonies that give rise to full-size colonies only if a reversion event occurs. Because two successive mutational events are required, mutator cells are favored to generate full-size colonies. Reversion of a third marker allows direct visualization of the mutator phenotype by the large number of blue papillae in the full-size colonies. We also describe plate selections involving three successive nutrient markers followed by a fourth papillation step. Different frameshift or base substitution mutations are used to select for mismatch-repair-defective strains (mutHLS and uvrD). We can detect and monitor mutator cells arising spontaneously, at frequencies lower than 10(-5) in the population. Also, we can measure a mutator cascade, in which one type of mutator (mutT) generates a second mutator (mutHLS) that then allows stepwise frameshift mutations. We discuss the relevance of mutators arising on a single medium as a result of cells overcoming successive growth barriers to the development and progression of cancerous tumors, some of which are mutator cell lines.  (+info)

Steady-state nitrogen isotope effects of N2 and N2O production in Paracoccus denitrificans. (3/6674)

Nitrogen stable-isotope compositions (delta15N) can help track denitrification and N2O production in the environment, as can knowledge of the isotopic discrimination, or isotope effect, inherent to denitrification. However, the isotope effects associated with denitrification as a function of dissolved-oxygen concentration and their influence on the isotopic composition of N2O are not known. We developed a simple steady-state reactor to allow the measurement of denitrification isotope effects in Paracoccus denitrificans. With [dO2] between 0 and 1.2 microM, the N stable-isotope effects of NO3- and N2O reduction were constant at 28.6 per thousand +/- 1.9 per thousand and 12.9 per thousand +/- 2.6 per thousand, respectively (mean +/- standard error, n = 5). This estimate of the isotope effect of N2O reduction is the first in an axenic denitrifying culture and places the delta15N of denitrification-produced N2O midway between those of the nitrogenous oxide substrates and the product N2 in steady-state systems. Application of both isotope effects to N2O cycling studies is discussed.  (+info)

Sensitivity, specificity, and predictive values of three Salmonella rapid detection kits using fresh and frozen poultry environmental samples versus those of standard plating. (4/6674)

To reduce human exposure to Salmonella spp. in poultry products, broiler chicken flocks have been tested by culture methods. Since the standard techniques may take 3 to 5 days, rapid detection methods have been developed. In this study we tested the performance of three rapid tests originally developed for food samples by using environmental samples obtained from poultry houses. These rapid tests were Reveal, an enzyme-linked immunosorbent assay from Neogen Corp.; BIND, a bacterial ice nucleation detection method from Idetek Corp.; and a filter monitor method from Future Medical Technologies, Inc. For the standard culture, brilliant green with novabiocin and xylose-lysine-tergitol-4 agar were used for presumptive identification, and identities were confirmed by using poly-O antisera. Environmental samples were collected from farms belonging to an integrated poultry company prior to chick placement and 1 week before slaughter. Sensitivities, specificities, and predictive values with 95% confidence intervals were calculated. Statistical differences were determined by using McNemar's chi square test. The sensitivities of the different tests were not stable, varying widely between sample times, and were affected by freezing of the samples. All of the rapid tests had low sensitivities, which led to many false-negative results. All tests were able to detect Salmonella spp. at a concentration of 10 CFU/ml in at least one of four trials. The BIND and Reveal tests were simple to use with multiple samples and reduced laboratory time by up to 1 day. Based on our results, we do not recommend that any of these rapid tests, in their present state of development, be utilized with environmental samples collected with drag swabs.  (+info)

Rapid identification of Actinomycetaceae and related bacteria. (5/6674)

Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.  (+info)

Risk factors for lower airway bacterial colonization in chronic bronchitis. (6/6674)

The aim of this study was to determine the prevalence and risk factors for lower airway bacterial colonization (LABC) in stable chronic bronchitis (CB). Forty-one outpatients with CB were enrolled in the study (age 63.8+/-9.1 yrs (mean+/-SD); forced expiratory volume in one second (FEV1)/forced vital capacity (FVC) 62.8+/-11.2; current/former smokers 24/17). All patients had normal chest radiographs and an indication for performing fibreoptic bronchoscopy (pulmonary nodule, remote haemoptysis). The protected specimen brush (PSB) was used for bacterial sampling, and concentrations > or = 1,000 colony-forming units (cfu) x mL(-1) were considered positive for LABC. The repeatability of the procedure in CB was assessed in a random subsample of 18 subjects. A 72.2% quantitative agreement was found in the repeatability assessment of the PSB technique. Positive PSB cultures, obtained in 9 out of 41 (22%) patients, mainly yielded Haemophilus influenzae. The logistic regression model, used to determine which variables were related to colonization, showed that LABC was associated with current smoking (odds ratio (OR) 9.83, confidence interval (CI) 1.16-83.20) and low FVC (OR 0.73, CI 0.65-0.81). Age and FEV1 were not related to LABC. It was concluded that the prevalence of LABC in stable CB is high (22%), and current smoking is an important risk factor.  (+info)

Predisposing factors to bacterial colonization in chronic obstructive pulmonary disease. (7/6674)

The aim of this prospective observational study was to determine those factors influencing bacterial colonization in patients with stable chronic obstructive pulmonary disease (COPD). Eighty-eight outpatients with stable COPD and 20 patients with normal spirometry and chest radiography (controls) had a fibreoptic bronchoscopy performed with topical aerosol anaesthesia. Bacterial colonization was determined using the protected specimen brush (PSB) with a cut-off > or = 10(3) colony-forming units (CFU x mL(-1)). The influence of age, degree of airflow obstruction, smoking habit, pack-yrs of smoking, and chest radiographic findings on bacterial colonization were assessed by univariate and multivariate analysis. Significant bacterial growth was found in 40% of patients and in none of the controls. Haemophilus influenzae, Streptococcus viridans, S. pneumoniae and Moraxella catarrhalis were the most frequent pathogens. After adjustment for other variables, severe airflow limitation (odds ratio (OR) 5.11, 95% confidence interval (CI) 1.45-17.9) and current smoking (OR 3.17, 95% CI 2.5-8) remained associated with positive bacterial cultures. When only potentially pathogenic micro-organisms were considered, significant bacterial growth was found in 30.7% of patients, with severe airflow obstruction (OR 9.28, 95% CI 2.19-39.3) being the only variable independently associated with positive bacterial cultures. Our results show that stable chronic obstructive pulmonary disease patients have a high prevalence of bacterial colonization of distal airways which is mainly related to the degree of airflow obstruction and cigarette smoking.  (+info)

Extensive cross-contamination of specimens with Mycobacterium tuberculosis in a reference laboratory. (8/6674)

A striking increase in the numbers of cultures positive for Mycobacterium tuberculosis was noticed in a mycobacterial reference laboratory in Campinas, Sao Paulo State, Brazil, in May 1995. A contaminated bronchoscope was the suspected cause of the increase. All 91 M. tuberculosis isolates grown from samples from patients between 8 May and 18 July 1995 were characterized by spoligotyping and IS6110 fingerprinting. Sixty-one of the 91 isolates had identical spoligotype patterns, and the pattern was arbitrarily designated S36. The 61 specimens containing these isolates had been processed and cultured in a 21-day period ending on 1 June 1995, but only 1 sample was smear positive for acid-fast bacilli. The patient from whom this sample was obtained was considered to be the index case patient and had a 4+ smear-positive lymph node aspirate that had been sent to the laboratory on 10 May. Virtually all organisms with spoligotype S36 had the same IS6110 fingerprint pattern. Extensive review of the patients' charts and investigation of laboratory procedures revealed that cross-contamination of specimens had occurred. Because the same strain was grown from all types of specimens, the bronchoscope was ruled out as the outbreak source. The most likely source of contamination was a multiple-use reagent used for specimen processing. The organism was cultured from two of the solutions 3 weeks after mock contamination. This investigation strongly supports the idea that M. tuberculosis grown from smear-negative specimens should be analyzed by rapid and reliable strain differentiation techniques, such as spoligotyping, to help rule out laboratory contamination.  (+info)

Bacteriological techniques refer to the various methods and procedures used in the laboratory for the cultivation, identification, and study of bacteria. These techniques are essential in fields such as medicine, biotechnology, and research. Here are some common bacteriological techniques:

1. **Sterilization**: This is a process that eliminates or kills all forms of life, including bacteria, viruses, fungi, and spores. Common sterilization methods include autoclaving (using steam under pressure), dry heat (in an oven), chemical sterilants, and radiation.

2. **Aseptic Technique**: This refers to practices used to prevent contamination of sterile materials or environments with microorganisms. It includes the use of sterile equipment, gloves, and lab coats, as well as techniques such as flaming, alcohol swabbing, and using aseptic transfer devices.

3. **Media Preparation**: This involves the preparation of nutrient-rich substances that support bacterial growth. There are various types of media, including solid (agar), liquid (broth), and semi-solid (e.g., stab agar). The choice of medium depends on the type of bacteria being cultured and the purpose of the investigation.

4. **Inoculation**: This is the process of introducing a bacterial culture into a medium. It can be done using a loop, swab, or needle. The inoculum should be taken from a pure culture to avoid contamination.

5. **Incubation**: After inoculation, the bacteria are allowed to grow under controlled conditions of temperature, humidity, and atmospheric composition. This process is called incubation.

6. **Staining and Microscopy**: Bacteria are too small to be seen with the naked eye. Therefore, they need to be stained and observed under a microscope. Gram staining is a common method used to differentiate between two major groups of bacteria based on their cell wall composition.

7. **Biochemical Tests**: These are tests used to identify specific bacterial species based on their biochemical characteristics, such as their ability to ferment certain sugars, produce particular enzymes, or resist certain antibiotics.

8. **Molecular Techniques**: Advanced techniques like PCR and DNA sequencing can provide more precise identification of bacteria. They can also be used for genetic analysis and epidemiological studies.

Remember, handling microorganisms requires careful attention to biosafety procedures to prevent accidental infection or environmental contamination.

Bowhill, Thomas (1899). Manual of bacteriological technique and special bacteriology. New York: W. Wood & company. p. 17. ...
Petri dish Kolle culture flask John W. Eyre (1913): Bacteriological Techniques: A Laboratory Guide. Reprinted as Volume 39 of ... Sophie A. Leliévre (2015): "Cell Culture Basics 11: Adding medium to a culture flask with attached cells". Lab technique ...
In 1911, Avery instructed staff of H. K. Mulford Company in bacteriological techniques, and they taught him the industrial ... They used available techniques to remove various macromolecules - proteins, RNA, and DNA - from the bacteria. Then, the killed ... he performed medical research with bacteriological, immunological, and chemical approaches. For a period of time, White came ...
... ing is a bacteriological laboratory technique used to differentiate bacterial species into two large groups (gram- ... Gram devised his technique not for the purpose of distinguishing one type of bacterium from another but to make bacteria more ... The Wikibook School Science has a page on the topic of: Gram staining Gram staining technique video (CS1: long volume value, ... The method is named after its inventor, the Danish scientist Hans Christian Gram (1853-1938), who developed the technique while ...
The Ziehl-Neelsen stain, also known as the acid-fast stain, is a bacteriological staining technique used in cytopathology and ... Microbiology techniques, Laboratory techniques, Histopathology, Histotechnology, Staining dyes, Staining, Bacteriology, Acid- ... Another acid-fast stain was developed by Joseph Kinyoun by using the Ziehl-Neelsen staining technique but removing the heating ... Kinyoun modification (or cold Ziehl-Neelsen technique) is also available. A protocol in which a detergent is substituted for ...
... sophisticated bacteriological and virological techniques allowed the production of significant stockpiles of weaponized bio- ...
... bacteriological techniques MeSH E05.200.875.150.125 - bacterial typing techniques MeSH E05.200.875.150.125.150 - bacteriophage ... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ...
The Elements of Bacteriological Technique (1902) several later editions Serums, Vaccines and Toxines in Treatment and Diagnoses ... late Director of Bacteriological Depart, Guy's Hospital, and Lecturer on Bacteriology in the Medical School , WHO'S WHO & WHO ...
Microbiology techniques, Water quality indicators). ... Bacteriological water analysis is a method of analysing water ...
Some chemical techniques may be implemented using nanomaterials. Physical treatment techniques include, but are not limited to ... Contaminants found in groundwater cover a broad range of physical, inorganic chemical, organic chemical, bacteriological, and ... Most ground water treatment techniques utilize a combination of technologies. Some of the biological treatment techniques ... Chemical oxidation has proven to be an effective technique for dense non-aqueous phase liquid or DNAPL when it is present.[ ...
He invented a technique to stain bacteria so that they could be studied under the light microscope, and from around 1920, ... The commission tested bacteriological and histochemical stains from the fledgling American dye industry, brought into existence ... He wrote two biographies of his father, Herbert William Conn: one appears in Bacteriological Reviews and deals with the ... He served as president of the Society of American Bacteriologists in 1948, and also chaired the society's techniques committee ...
A bacteriological laboratory was added two years later. In 1893 when a typhoid epidemic (Salmonella typhi) arose along the ... During this time, Sedgwick and students invented techniques for identifying and quantitatively analyzing the microorganisms in ...
Belgian fire fighter academies have begun adapting newer techniques, such as the Swedish techniques for structural firefighting ... It also ensures public safety in terrorist emergencies such as chemical, bacteriological, radiological and nuclear attacks. In ... Most of the biggest Lithuania cities are equipped with the newest gear and technique. Lithuania firefighters vehicles are ...
Techniques such as aeration, flocculation, and granular activated carbon absorption could combat this and these techniques were ... As understanding of the bacteriological nature of disease became clearer in the late 19th century the need to filter and treat ... In Lowell, MA after a typhoid outbreak in 1890 William Thompson Sedgwick applied bacteriological methods to the investigation ... Drinking water regulations were enacted by the US federal government beginning in 1914 regarding the bacteriological quality of ...
In this technique the number of viral plaques formed by a viral inoculum is counted, from which the actual virus concentration ... Bacteriological water analysis, Clonogenic assays, ... Bisphenol F Aquatic toxicity tests An MBAS assay indicates anionic ... A wide range of cellular secretions (say, a specific antibody or cytokine) can be detected using the ELISA technique. The ... This includes a detailed, technical explanation of contemporaneous metallic ore assay techniques. The dictionary definition of ...
On February 14, 1970, a presidential order outlawed all stockpiles of bacteriological weapons and nonliving toxins. However, ... A 1967 CIA memo which was uncovered by the Church Committee contained evidence of at least three covert techniques for ...
Rodríguez Vargas was there as an educator and investigator of the bacteriological aspects of dental diseases. His research led ... Rodríguez Vargas also developed the techniques and methods of analysis. On September 28, 1928, Rodriguez Vargas published in ...
Bacteriological and clinical studies on the biology of "gonococcus Neisser". Ueber Uterus-Gonorrhöe, Verhandlungen der ... "The technique of vagino-peritoneal operations" (1907). Biography of Ernst Wertheim @ Who Named It Ehrengrab Ernst Wertheim am ... where he devoted himself to developing surgical techniques for the treatment of uterine prolapse. Wertheim died in 1920 in ...
Other techniques (such as X-rays, CAT scans, PET scans or NMR) are used to produce images of internal abnormalities resulting ... Bacteriological plates such as these are commonly used in the clinical identification of infectious bacterium. Microbial ... This technique is especially useful in the diagnosis of viral diseases, where the light microscope is incapable of identifying ... Aseptic technique was introduced in medicine and surgery in the late 19th century and greatly reduced the incidence of ...
Rather than punch through heavy paper to create the raised dots of the Braille alphabet for the blind, these techniques apply ... Rodríguez Vargas was assigned to the Army Dental Corps as an educator and investigator of the bacteriological aspects of dental ... She is also responsible for planning experimental studies to validate analytical techniques and study solar sails dynamics. ... Sanchez used a new technique called TechnoPrint and TechnoBraille. ...
He was later asked to build the Colmslie factory, which had a well-equipped chemical and bacteriological laboratory. His son, ... He had designed the revolutionary ore-extraction technique of the "skin" or "film" flotation process, patented in 1905, to deal ...
Cox eschewed the technique because of the danger monkey virus represented. In October, 1952, Cox reported that he had grown the ... Bacteriological Reviews. 25 (4): 383-388. doi:10.1128/br.25.4.383-388.1961. PMC 441121. PMID 13881902. Furesz J (2006). " ... Although Cox's egg technique was in widespread use by 1943, it had not been successful for polio. In 1947, John Franklin Enders ...
Pasteur had not used attenuation, but secretly used Toussaint's technique.) In 1883, responding to a cholera epidemic in ... his bacteriological followers regarded a bacterial species' properties as unalterable, Pasteur's modification of virulence to ... Koch had transformed bacteriology by introducing the technique of pure culture, whereby he established the microbial cause of ... In July 1880 Toussaint reported developing a technique of chemical deactivation to produce anthrax vaccine that successfully ...
This stain is still a standard technique to classify bacteria and make them more visible under a microscope to this day. ... Graduate studies also provide opportunities for practical experience in applying bacteriological concepts to a work environment ... This discovery later led to himself and others inventing antiseptic technique, vaccinations, and germ theory. Sir William ...
Also known as the Convention on the Prohibition of Military or Any Other Hostile Use of Environmental Modification Techniques. ... Also known as the Convention on the Prohibition of the Development, Production and Stockpiling of Bacteriological (Biological) ...
Jones, D; Sneath, P H (March 1970). "Genetic transfer and bacterial taxonomy". Bacteriological Reviews. 34 (1): 40-81. doi: ... New Techniques". Plant Physiology. 173 (1): 65-78. doi:10.1104/pp.16.01340. PMC 5210733. PMID 27895205. Sessa, Emily B.; Zimmer ... Bacteriological Reviews. 32 (1): 55-83. doi:10.1128/br.32.1.55-83.1968. PMC 378292. PMID 4869941. Anderson, E S (October 1968 ...
She conducted bacteriological investigations of commonly occurring diseases in domestic animals. Among her achievements were: ... Love's research associated with EHV1 and EHV4 involved using virological and molecular biological techniques to understand the ...
A general bacteriological laboratory, an incubating room, mixing, distributing, and sterilizing rooms, and a separate room ... Facilities will also be afforded for acquiring the technique necessary for the manufacture of these products, and also for ...
From 1893 he studied biological staining techniques with Victor Morax (1866-1935), publishing two papers on the staining ... he resigned his post at the bacteriological institute of Constantinople and returned to the Pasteur Institute. At the Pasteur ...
In: MA Lang, CC Baldwin (Eds.) The Diving for Science…1996, "Methods and Techniques of Underwater Research"". Proceedings of ... bacteriological aspects". Aerosp Med. 41 (6): 611-6. PMID 4392833. Cobet AB, Dimmick RL (June 1970). "Tektite-I program: ... and space resource-sharing A goal of the Tektite program was to prove that saturation diving techniques in an underwater ...
Bowhill, Thomas (1899). Manual of bacteriological technique and special bacteriology. New York: W. Wood & company. p. 17. ...
Bacteriological Techniques * Biofilms / growth & development * Endocarditis, Bacterial / microbiology * Enterococcus faecalis ...
The Elements of Bacteriological Technique , John William Henry Eyre. *. Strain the mixture through butter muslin into a clean ...
Bacteriological Techniques / methods* * Birds / microbiology* * Chickens / microbiology* * DNA, Bacterial / chemistry * DNA, ...
Bacteriological techniques for testing carpets. Health Lab Sci. 1973;10:28-38.PubMedGoogle Scholar ...
E. coli strains, plasmid DNAs, and bacteriological techniques.. All E. coli strains used in this study are derivatives of ...
The Elements of Bacteriological Technique , John William Henry Eyre. *. During the implantation of conjugial love, the love of ...
Categories: Bacteriological Techniques Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
Covers new main installations, proper flushing techniques, disinfection procedures, and bacteriological testing. ... Flushing, Disinfection, and Bacteriological Testing (EL211). 2 hours self-paced ... Course 2 explores data & analytical techniques to evaluate corrosion control practice and recognize when additional evaluation ...
New eBook discusses analytical techniques for w... How do new EPA drinking water regulations defin... ...
A laboratory manual of parasitological and bacteriological techniques. Geneva, World Health Organization, 1996. ...
In this webinar you will learn critical parameters for controlling water quality, bacteriological analysis techniques and ... Webinar] Bacteriological Analysis of Water. Waterborne diseases are a leading cause of death and a global concern. With the ... Sign-up Now: Bacteriological Analysis of Water October 14, 2020. ... COVID 19 pandemic, bacteriological monitoring of water quality ...
4.8.1.1 Pour-Plate and Spread-Plate Techniques........................................... 115 4.8.1.2 Membrane Filtration ... Multiple Tube Technique) ........ 112 4.7.1.3 Presence-Absence Test ... 118 4.8.2.3 Membrane Filtration Technique.......................................................... 119 4.8.2.4 Determination ...
Bacteriological techniques. Rights: Copyright © 2016 Glasson et al. This is an open-access article distributed under the terms ...
... pleuropneumoniae using bacteriological and serological techniques, respectively. A. pleuropneumoniae was detected in the ... pleuropneumoniae using bacteriological and serological techniques, respectively. A. pleuropneumoniae was detected in the ... pleuropneumoniae using bacteriological and serological techniques, respectively. A. pleuropneumoniae was detected in the ... pleuropneumoniae using bacteriological and serological techniques, respectively. A. pleuropneumoniae was detected in the ...
Bacteriological Techniques (1967-1988). Microbiological Techniques (1978-1988). Public MeSH Note:. 89. ...
Bacteriological Analysis. Bacteriological Techniques. Bioaccumulation. Bioaccumulation. Biological Analysis. Biological ...
Bacteriological Techniques * Cattle * DNA, Bacterial * Dairy Cattle * Female * Mastitis * Mastitis, Bovine * Methicillin ...
Bacteriological techniquesNLM classification: QW 25 Tags from this library: No tags from this library for this title. Log in to ... Techniques;Publication details: Auckland : MEDSI/McGraw-Hill, 1988. Description: 295 pISBN: 2864391619Subject(s): ...
MeSH headings : Bacteria / growth & development; Bacteria / isolation & purification; Bacteriological Techniques / methods; ...
... a culture of bacteria containing no other bacterial species in quantities to be detected by normal bacteriological techniques. ... The resulting plasmid is mobilized from E. coli to Pseudomonas fluorescens AIP0069 by conjugation, a technique well known in ... There are many techniques for identifying these genes and classifying new sequences in a previous framework (see, for example, ... A suitable gene is amplified by PCR or made synthetically using techniques well known in the art. The open reading frame is ...
The Elements of Bacteriological Technique • John William Henry Eyre. ... tie with Mrs. Pargeter grew, if not more passionate, ...
The Elements of Bacteriological Technique A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition ...
... as well as variations in bacteriological techniques and specimen used. In some studies, like the present one, only stool ... the technique is very expensive, requiring a well-equipped laboratory and not readily available in many health facilities in ...
AntibodiesAntigensBacterialBacteriological_TechniquesCross_ReactionsHumansImmunologic_TechniquesMeningococcal_VaccinesNeisseria ... Analysis,BacterialBacterial,BacteriologicalCapsules,ChainData,FalseHaemophilusHumans,Infections,Insertional,Molecular ... Mutagenesis,NegativePolymerasePrimers,Reaction,Reactions,SensitivitySequenceSpecificity,Techniques,andbacterial_capsules ...
were performed using standard bacteriological techniques and PCR. Antibiotic susceptibility test was performed using disc ... On incorporation in chitosan, the polymer nanocomposite films were developed by solvent casting technique. The developed films ... Taken together, Raman spectroscopy is a powerful metabolic fingerprinting technique for simultaneously revealing the AMR ...
the elements of bacteriological technique john william henry eyre. collections in churches on sunday, june 9th, aggregated $ ...
Conduct bacteriological sampling and lab work using standard techniques; Maintain records and data in computerized form; ... Methods and techniques used to conduct a variety of analytical studies. Principles, practices, methods and techniques of report ... Methods and techniques used to conduct a variety of analytical studies. Principles, practices, methods and techniques of report ... Conduct bacteriological sampling and lab work using standard techniques; Maintain records and data in computerized form; ...
  • In this webinar you will learn critical parameters for controlling water quality, bacteriological analysis techniques and standard methods for bacterial enumeration, legionella risk and how to control it, tips for proper water sample collection, handling, and identification, and Corning solutions for water sampling, testing, and analysis. (corning.com)
  • Bacterial isolates were subjected to microscopic examination using Gram's staining technique and biochemical tests (e.g. coagulase, catalase, oxidase, and motility). (who.int)
  • The diagnosis of MB was confirmed through bacteriological examination and PCR results from skin slit smears. (bvsalud.org)
  • Bacteriological examination did not prove to be an efficient technique for the analysis of saliva and periodontium samples. (bvsalud.org)
  • PCR analysis of skin smears was more efficient at diagnosing PB patients than bacteriological examination. (bvsalud.org)
  • bacteriological examination. (bvsalud.org)
  • A detailed periodontal examination, including oral bacteriological examination, revealed localized severe periodontitis (stage III, grade B) in the maxillary left first and second molars and in the mandibular right second molar. (biomedcentral.com)
  • After consultation with the orthodontist, the orthodontic treatment was suspended based on the results of the bacteriological examination to allow for periodontal treatment. (biomedcentral.com)
  • A cross-sectional study was designed to use bacteriological (baciloscopy) and molecular (PCR) parameters to detect M. leprae in exudates of the gingival sulcus/periodontium pocket, saliva and skin slit smears from multiple clinical forms of leprosy patients without previous treatment. (bvsalud.org)
  • This technique was further evaluated by comparing numbers of visible acid-fast bacilli, slide-reading speed and reliability for triplicate smears before versus after bleach-sedimentation of sputum specimens (n = 25). (ox.ac.uk)
  • Diverse staining solutions are available for the detection of these pathogenic bacteria in histological tissue cultures and bacteriological smears. (sigmaaldrich.com)
  • To correlate bacteriological and molecular findings with clinical data and compare laboratory techniques. (bvsalud.org)
  • Molecular techniques are revolutionising the routine identification and characterisation of pathogens, opening new opportunities and challenges in all areas of clinical microbiology from clinical diagnostics to fundamental research. (wellcomeconnectingscience.org)
  • The intensive programme will provide clinical microbiologists and laboratory scientists working in Africa with a concise yet comprehensive overview of the latest research and practice in this essential area, with an emphasis on how these techniques can be applied day-to-day in an African setting, especially when resources are limited. (wellcomeconnectingscience.org)
  • Bacteriological investigations are essential in clinical diagnostics and industrial quality control. (sigmaaldrich.com)
  • According to WHO, all PTB +ve patients one of the useful indicators to determine the efficacy of should be evaluated for bacteriological status after the anti-tuberculosis treatment and essential in the clinical intensive phase to determine treatment outcomes ( 7 ). (who.int)
  • The course is aimed at those primarily trained in culture-based microbiology and combines an understanding of the latest research techniques and theoretical approaches, with practical methods to provide a foundation-level of understanding of the philosophy, methods, and specialist terminology of molecular techniques. (wellcomeconnectingscience.org)
  • Review sampling techniques and culture methods. (livistoinpractice.com)
  • Fluorescence detection methods are utilized with the Ziehl-Neelsen staining technique, via either heat-treated slides (hot staining) or non-heated treatment (cold staining). (sigmaaldrich.com)
  • Describe bacteriological findings in mastitis samples and different stages of lactation. (livistoinpractice.com)
  • Learn how to properly clean your laboratory glassware to improve your lab technique and insure high quality results. (sigmaaldrich.com)
  • A World Health Organization (WHO)/National Bacteriological Laboratory meeting was held in Stockholm, Sweden, from June 16 to June 18, 1982, to review rapid laboratory virus diagnosis, with special emphasis on coordination of production, quality control, and supply of reagents. (cdc.gov)
  • Periodontal and bacteriological examinations after treatment revealed regression of the localized periodontitis with bone regeneration. (biomedcentral.com)
  • Most importantly, this research has found that both bacteriological and molecular techniques can be used to detect ORT. (tci-thaijo.org)
  • Course 2 explores data & analytical techniques to evaluate corrosion control practice and recognize when additional evaluation is needed. (awwa.org)
  • New eBook discusses analytical techniques for w. (envirotech-online.com)
  • Bacteriological quality of recreational water includes heterotrophic bacteria, total coliforms and Escherichia coli according to Greek Hygienic Regulation. (iwaponline.com)
  • Gram staining is an essential staining technique in bacteriology used to differentiate Gram-positive and Gram-negative bacteria. (sigmaaldrich.com)
  • The aim of the current study is to describe and evaluate the bacteriological quality of 2,844 swimming pool and jacuzzi/spa water samples in northern Greece during the 2011-2016 period. (iwaponline.com)
  • The major advances in viral respiratory-disease diagnosis include the successful extension of immunofluorescence techniques to more laboratories, use of large-scale production of antibodies in eggs, and development of sensitive solid-phase immunoassays for detection of virus antigens in nasopharyngeal secretions. (cdc.gov)
  • These techniques will be explored in the lectures and tutorials and employed during the course practical sessions in the cross-cutting themes of pathogen detection and characterisation, including antimicrobial resistance and the evolution of pathogens, and the application of the data in the improvement of interventions to reduce disease burden. (wellcomeconnectingscience.org)
  • With the COVID 19 pandemic, bacteriological monitoring of water quality has become crucial. (corning.com)
  • 4.5 Bacteriological Water Quality Standards. (taylorfrancis.com)
  • It performs normal and less government sanctioned testing techniques that incorporate explicit synthetic, tiny and bacteriological research center tests. (hairisourthing.com)
  • No periodontium or saliva samples had positive bacteriological results. (bvsalud.org)
  • In the light of these results, the performance of 5 gravity bleach-sedimentation techniques that sterilise sputum specimens (n = 16) were compared. (ox.ac.uk)
  • A bacteriological culture may be performed using a throat swab, expectorated throat sputum, or throat washings the respiratory tract of patients with pharyngitis, croup, bronchitis, or pneumonia. (medscape.com)
  • Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technique for identification of Pseudomonas aeruginosa isolated from broilers and human. (ekb.eg)
  • The best-performing of these bleach-sedimentation techniques involved adding 1 volume of 5% bleach to 1 volume of sputum, shaking for 10-minutes, diluting in 8 volumes distilled water and sedimenting overnight before microscopy. (ox.ac.uk)
  • Techniques used in studying bacteria. (ouhsc.edu)
  • This study was undertaken to apply 16S rRNA sequence and Cellular Fatty Acid (CFA) composition analysis techniques for the identification and characterization of seven individual bacteria isolates obtained from seven infected fish samples. (scialert.net)
  • A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages. (lookformedical.com)
  • Diverse staining solutions are available for the detection of these pathogenic bacteria in histological tissue cultures and bacteriological smears. (merckmillipore.com)
  • METHODS AND RESULTS: A simple and rapid technique for screening of potential chitinolytic bacteria has been developed using the chitin binding dye calcofluor white M2R in chitin agar. (ox.ac.uk)
  • This technique also allowed a viable count of the bacteria to be made. (bath.ac.uk)
  • Please refer to MSU Extension Publication 3124 Collecting Milk Samples for Microbiological Analysis and Publication 3132 Using an On-Farm Bacteriological Culture System for tips on collecting aseptic samples and streaking samples for bacteria identification. (msstate.edu)
  • Bacteriological diagnosis of urinary tract infections]. (nih.gov)
  • The definitive diagnosis of TB depends on the bacteriological confirmation of MTB in the specimen sent for investigation by either culture or PCR technique. (pediatriconcall.com)
  • The diagnosis of MB was confirmed through bacteriological examination and PCR results from skin slit smears. (bvsalud.org)
  • The diagnosis of TB in OWC is based on various ante-mortem (intradermal tuberculin test, serology) and post-mortem (pathology and bacteriological culture) techniques. (biomedcentral.com)
  • Methodology: In this investigation, 35 Staphylococcus aureus isolates were obtained from patient infections' oral cavities and were thoroughly characterized and identified using standard bacteriological techniques, biochemical tests, and the VITEK-2 system compact. (hivnursing.net)
  • The organism was isolated and sub-cultured to obtain a pure culture, identified using conventional bacteriological techniques and API system. (envirobiotechjournals.com)
  • Sampling was performed by rectal swab and conventional bacteriological techniques were applied, for later implementation of the API 20E system and molecular identification by polymerase chain reaction (PCR). (ajvs.cl)
  • Wound swab was collected using sterile cotton swabs and processed for bacterial isolation and susceptibility testing to antimicrobial agents, acetic acid, hydrogen peroxide and dabkin solution following standard bacteriological techniques. (medscape.com)
  • Throat swabs were collected, and bacterial isolation and identification were done using standard bacteriological procedures. (bvsalud.org)
  • Bacteriological Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
  • Her primary research interests are identification, epidemiology, and molecular subtyping techniques concerning S. aureus , especially MRSA. (medscape.com)
  • A bacteriological culture may be performed using a throat swab, expectorated throat sputum, or throat washings the respiratory tract of patients with pharyngitis , croup , bronchitis , or pneumonia . (medscape.com)
  • For this purpose, water samples were collected from three different sites of Al-Chebaysh marshes located in Dhi Qar Governorate, south of Iraq in 2017 to investigate water bacteriological contamination. (dysona.org)
  • Preoperative US data, blood analyses and bacteriological tests of blood and vaginal inoculations were normal in every patient. (sages.org)
  • The whole herd was additionally tested using cellular and humoral based diagnostic techniques. (biomedcentral.com)
  • Critical study of reactions furnished by routine peripheral bacteriological sampling in newborn infants]. (nih.gov)
  • Bacteriological sampling is performed by manual techniques because of stringent sterilization requirements . (chempedia.info)
  • RESULTS Of the 20 samples of raw milk collected for bacteriological analysis , 3 samples were found to be of excellent quality, 5 were very good, 4 were good, 5 were fair and 3 were of poor quality. (bvsalud.org)
  • This study, therefore, was designed to evaluate bacteriological quality of milk samples collected from various localities within Ilesha metropolis. (bvsalud.org)
  • A major benefit of on-farm milk bacteriological culture systems is that results can be obtained within 24 hours compared to a week or more for samples sent off to a lab. (msstate.edu)
  • This publication discusses how to read results of on-farm bacteriological cultures grown on the Minnesota Easy® Culture System. (msstate.edu)
  • For bacteriological samples withdrawn from a tap, the water should mn for five minutes and then be shut off the tap should then be sterilized by flaming before a sample is taken. (chempedia.info)
  • Using of PCR technique revealed that there were one or more virulence genes in E.coli (stx1&stx2) strains isolated from the examined samples of chicken products. (ekb.eg)
  • Intraoperatively the peritoneal bacteriological tests and evaluation of the degree of adhesive process were performed. (sages.org)
  • Intraopertaively no adhesions in pelvic space were found and peritoneal bacteriological tests were negative. (sages.org)
  • A bacteriological study of Thiobacillus ferro oxidans. (bath.ac.uk)
  • This graph shows the total number of publications written about "Bacteriological Techniques" by people in this website by year, and whether "Bacteriological Techniques" was a major or minor topic of these publications. (ouhsc.edu)
  • We used conventional bacteriological and molecular techniques to identify and characterise meningococci. (hal.science)
  • Many of the basic Principles and techniques of modern bacteriology were adapted or devised by Koch, who therefore is often regarded as the chief founder of that science. (encyclopedia.com)
  • Is the traditional placement of stainless steel crowns in primary teeth more painful than the non-preparation Hall technique? (umassmed.edu)