Plasmids encoding bacterial exotoxins (BACTERIOCINS).
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. No endospores are produced. Its organisms are found in fermenting plant products and are nonpathogenic to plants and animals, including humans.
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.
A 34-amino acid polypeptide antibiotic produced by Streptococcus lactis. It has been used as a food preservative in canned fruits and vegetables, and cheese.
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The functional hereditary units of BACTERIA.
A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.
A family of gram-positive bacteria found regularly in the mouth and intestinal tract of man and other animals, in food and dairy products, and in fermenting vegetable juices. A few species are highly pathogenic.
A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.
Proteins found in any species of bacterium.
A species of gram-positive, rod-shaped bacteria isolated from the intestinal tract of humans and animals, the human mouth, and vagina. This organism produces the fermented product, acidophilus milk.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
A gram-positive, non-spore-forming group of bacteria comprising organisms that have morphological and physiological characteristics in common.
A genus of gram-positive, rod-shaped bacteria whose cells occur singly, in pairs or short chains, in V or Y configurations, or in clumps resembling letters of the Chinese alphabet. Its organisms are found in cheese and dairy products as well as on human skin and can occasionally cause soft tissue infections.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.
Vertical transmission of hereditary characters by DNA from cytoplasmic organelles such as MITOCHONDRIA; CHLOROPLASTS; and PLASTIDS, or from PLASMIDS or viral episomal DNA.
A bacteriocin produced by a plasmid that can occur in several bacterial strains. It is a basic protein of molecular weight 56,000 and exists in a complex with its immunity protein which protects the host bacterium from its effects.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.
A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Substances that reduce the growth or reproduction of BACTERIA.
Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.
A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
A natural association between organisms that is detrimental to at least one of them. This often refers to the production of chemicals by one microorganism that is harmful to another.
A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Bacteria which retain the crystal violet stain when treated by Gram's method.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
A species of rod-shaped, LACTIC ACID bacteria used in PROBIOTICS and SILAGE production.
A genus of GRAM-POSITIVE ENDOSPORE-FORMING RODS in the family Paenibacillaceae. Most strains have been isolated from the natural environment, particularly soils.
A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes rotting, particularly of storage tissues, of a wide variety of plants and causes a vascular disease in CARROTS; and POTATO plants.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
The sum of the weight of all the atoms in a molecule.
Bacteriocins elaborated by mutant strains of Bacillus megaterium. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same species.
A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.
Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.

Isolation and characterization of ColE1-derived plasmid copy-number mutant. (1/196)

The plasmid pBGP120 is a ColE1 derivative that contains elements of the Escherichia coli lac operon and the Tn3 transposon. We have selected and isolated a copy-number mutant of pBGP120. In exponentially growing cultures, the copy-number mutant, pOP1, represents approximately 30% of total intracellular DNA compared to about 5% for pBGP120. Plasmid-encoded beta-galactosidase monomer can represent 50% of newly synthesized protein in cells carrying pOP1. pOP1 is structurally unstable in certain genetic backgrounds and under certain growth conditions, breaking down to a smaller sized plasmid that retains the DNA overproducer phenotype and the Tn3 transposon. The smaller overproducer plasmid, pOP1delta6, is generated by a continuous deletion of sequences located between one end of the Tn3 transposon and a site about 630 nucleotides from the EcoRI site in the beta-galactosidase structural gene of pOP1. pOP1delta6 retains the ColE1 origin of replication but has lost the lac promotor and operator and most of the beta-galactosidase structural gene. pOP1delta6 exists at approximately 210 copies per chromosome in exponentially growing cells.  (+info)

Analysis of the CoIE1 stability determinant Rcd. (2/196)

Multimer formation is an important cause of instability for many multicopy plasmids. Plasmid CoIE1 is maintained stably because multimers are converted to monomers by Xer-mediated site-specific recombination at the cer site. However, multimer resolution is not the whole story; inactivation of a promoter (Pcer) within cer causes plasmid instability even though recombination is unaffected. The promoter directs the synthesis of a short transcript (Rcd) which is proposed to delay the division of multimer-containing cells. Mapping of the 5' terminus of Rcd confirms that transcription initiates from Pcer. The 3' terminus shows considerable heterogeneity, consistent with a primary transcript of 95 nt being degraded via intermediates of 79 and 70 nt. Secondary structure predictions for Rcd are presented. Of four mutations which abolish Rcd-mediated growth inhibition, one reduces the activity of Pcer while the other three map to the rcd coding sequence and reduce the steady-state level of the transcript. RNA folding analysis suggests that these three mutant transcripts adopt a common secondary structure in which the major stem-loop differs from that of wild-type Rcd. A survey of 24 cer-like multimer resolution sites revealed six which contain Pcer-like sequences. The putative transcripts from these sites have similar predicted secondary structures to Rcd and contain a highly conserved 15 base sequence. To test the hypothesis that Rcd acts as an anti-sense RNA, interacting with its target gene(s) through the 15 nt sequence, we used DNA hybridization and sequence analysis to find matches to this sequence in the Escherichia coli chromosome. Our failure to find plausible anti-sense targets has led to the suggestion that Rcd may interact directly with a protein target.  (+info)

Expression of leading region genes on IncI1 plasmid ColIb-P9: genetic evidence for single-stranded DNA transcription. (3/196)

The leading region of a plasmid is the first sector to enter the recipient cell in bacterial conjugation. This sector of IncI1 plasmid ColIb-P9 includes genes that are transcribed in a transient pulse early in the conjugatively infected cell to promote establishment of the immigrant plasmid. Evidence is presented that the burst of gene expression is regulated by a process which is independent of a repressor but dependent on the orientation of the genes on the unique plasmid strand transferred in conjugation. The nucleotide sequence of 11.7 kb of the leading region was determined and found to contain 10 ORFs; all are orientated such that the template strand for transcription corresponds to the transferred strand. The leading region contains three dispersed repeats of a sequence homologous to a novel promoter in ssDNA described by H. Masai & K. Arai (1997, Cell 89, 897-907). It is proposed that the repeats are promoters that form in the transferring strand of ColIb to support transient transcription of genes transferred early in conjugation.  (+info)

Features of distamycin preferential binding sites on natural DNA predicted using differential scanning calorimetry. (4/196)

The interaction of distamycin with ColE1 DNA was examined by using differential scanning calorimetry (DSC) taking the helix-coil transition theory of DNA into consideration. Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends.  (+info)

Monomer-dimer control of the ColE1 P(cer) promoter. (5/196)

XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), located centrally within cer, is also required for stable plasmid maintenance. P(cer) is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P(cer) in plasmid monomers. P(cer) is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this study has demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P(cer) activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally.  (+info)

Purification and characterization of a proteolytic active fragment of DNA topoisomerase I from the brine shrimp Artemia franciscana (Crustacea Anostraca). (6/196)

The ATP-independent type I topoisomerase from the crustacean Artemia franciscana was purified to near-homogeneity. Its activity was measured by an assay that uses the formation of an enzyme-cleaved DNA complex in the presence of the specific inhibitor camptothecin. The purification procedure is reported. Purified topoisomerase is a single-subunit enzyme with a molecular mass of 63 kDa. Immunoblot performed on the different steps of purification shows that the purified 63 kDa peptide is a proteolytic fragment of a protein with a molecular mass of 110 kDa. Similarly to the other purified eukaryotic topoisomerases, the crustacean enzyme does not require a bivalent cation for activity, but is stimulated in the presence of 10 mM-MgCl2; moreover, it can relax both negative and positive superhelical turns. The enzyme activity is strongly inhibited by the antitumour drug camptothecin. The enzyme inhibition is related to the stabilization of the cleavable complex between topoisomerase I and DNA.  (+info)

Structure of the ColE1 DNA molecule before segregation to daughter molecules. (7/196)

The segregation of daughter DNA molecules at the end stage of replication of plasmid ColE1 was examined. When circular ColE1 DNA replicates in a cell extract at a high KCl concentration (140 mM), a unique class of molecules accumulates. When the molecule is cleaved by a restriction enzyme that cuts the ColE1 DNA at a single site, an X-shaped molecule in which two linear components are held together around the origin of DNA replication is made. For a large fraction of these molecules, the 5' end of the leading strand remains at the origin and the 3' end of the strand is about 30 nucleotides upstream of the origin. The 3' end of the lagging strand is located at the terH site (17 nucleotides upstream of the origin) and the 5' end of the strand is a few hundred nucleotides upstream of the terH site. Thus the parental strands of the molecule intertwine with each other only once. When the KCl concentration is lowered to 70 mM, practically all of these molecules are converted to daughter circular monomers or to catenanes consisting of two singly interlocked circular units.  (+info)

A new colicin that adsorbs to outer-membrane protein Tsx but is dependent on the tonB instead of the tolQ membrane transport system. (8/196)

A new colicin, Col5, was synthesized by an Escherichia coli isolate of human origin from the ECOR Collection. It was unique because it adsorbed to the outer-membrane protein Tsx, but used the tonB rather than the tolQ membrane transport system, which is employed by the only other Tsx-specific colicin, ColK. Col5 was encoded by a 5.2 kb plasmid, p5. It was inducible by mitomycin C, and strains harbouring p5 exhibited quasi-lysis. The bactericidal protein had an M(r) of 56,000.  (+info)

Colicins, a class of antimicrobial compounds produced by bacteria, are thought to be important mediators of intra- and interspecific interactions, and are a significant factor in maintaining microbial diversity. Colicins B and M are among the most common colicins produced by Escherichia coli, and are usually encoded adjacently on the same plasmid. In this study, the characterization of a collection of E. coli isolated from Australian vertebrates revealed that a significant fraction of colicin BM strains lack an intact colicin B activity gene. The colicin B and M gene region was sequenced in 60 strains and it was found (with one exception) that all plasmids lacking an intact colicin B activity gene have an identical colicin gene structure, possessing a complete colicin B immunity gene and a 130 bp remnant of the B activity gene. A phylogenetic analysis of the colicin M and B operons and characterization of the plasmids suggested that ColBM plasmids with a truncated B activity gene have evolved on at
Evidence is presented that ColV plasmid-mediated iron uptake, an important component of the virulence of invasive strains of Escherichia coli, is independent of colicin V synthesis and activity. A mutant of E. coli K-12 deficient in the biosynthesis of enterochelin (strain AN1937) was unable to grow on minimal agar containing the chelating agent α,α′-dipyridyl unless it was harboring the plasmid ColV-K30 (strain LG1315). Acquisition of the active plasmid-specified iron sequestering system was accompanied by marked enhancement of pathogenicity in experimental infections of mice. Mutants of strain LG1315 were isolated that were defective in iron uptake due to plasmid mutations. They were unchanged with respect to colicin production, but were significantly less virulent than the parent strain. Conversely, mutants isolated as defective in colicin V synthesis were normal for the plasmid-coded iron uptake mechanism and showed the same lethality for infected mice as did strain LG1315. Furthermore, ...
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
Colicins are plasmid-encoded antibiotics that are produced by and kill Escherichia coli and other related species. The frequency of colicinogeny is high, on average 30% of E. coli isolates produce colicins. Initial observations from one collection of 72 strains of E. coli (the ECOR collection) suggest that resistance to colicin killing is also ubiquitous, with over 70% of strains resistant to one or more colicins. To determine whether resistance is a common trait in E. coli, three additional strain collections were surveyed. In each of these collections levels of colicin production were high (from 15 to 50% of the strains produce colicins). Levels of colicin resistance were even higher, with most strains resistant to over 10 colicins. A survey of 137 non-E. coli isolates revealed even higher levels of resistance. We discuss a mechanism (pleiotropy) that could result in the co-occurrence of such high levels of colicin production and colicin resistance ...
Mindszent - A mindszenti horg sz, G l Istv n a k zelm ltban ritkas gnak sz m t 3,86 kil s m rn t fogott a Tisz n a Kurca-torkolatn l, m lt szombaton pedig egy 9 kil s amurt. Sikereinek titka speci lis etet anyaga.
Dr. Roz and Kil are relationship coaches and the creators of Marriage Exposed. They have been coaching couples and families for over 5 years. With a mixture of therapeutical strategies, biblical principals, practicality and laughter, Dr. Roz and Kil not only coach couples through the hard times of their relationships but they continue to impart wisdom, after their storms have passed ...
Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
A Tn5-based transposon bearing the kil gene (killing protein), mediating controlled export of periplasmic proteins into the culture medium, was constructed (Tn5-KIL3). This transposon contained the kil gene of the ColE1 plasmid under the growth-phase-dependent promoter of the fic gene (filamentation induced by cAMP) of Escherichia coli, an interposon located upstream of kil, a kanamycin/neomycin-resistance gene, a multiple cloning site and the mob site. The transposition of Tn5-KIL3 to Acetobacter methanolicus showed a moderate transposition frequency (10(-5)-10(-6)). By insertion of a Bacillus hybrid beta-glucanase (bgl) as a model protein into the transposon (Tn5-LF3) it was shown that the secretion function as well as the gene of the target protein had been transferred to and stably integrated into the chromosome of A, methanolicus, and that the transposition of Tn5-LF3 was non-specific. beta-Glucanase was highly overexpressed and secreted into the medium during stationary phase. Total and ...
How is intermediate-conductance K+ channel abbreviated? i-K+ stands for intermediate-conductance K+ channel. i-K+ is defined as intermediate-conductance K+ channel rarely.
The actions of the opioid agonist U50488H on I-A and I-K were examined in acutely isolated mouse hippocampal CA3 pyramidal neurons using the whole-cell patch clamp technique. U50488H caused a concentration dependent, rapidly developing and reversible inhibition of voltage-activated I-A and I-K. The inhibitory actions were still observed in the presence of 30 muM naloxone or 5 muM nor-binaltorphimine dihydrochloride. The IC50 values for the blockade of I-A and I-K were calculated as 20.1.9 and 3.7 muM, respectively. In the presence of 3.3 muM U50488H, repetitive stimulation induced use-dependent inhibition of I-A and I-K. A 10 muM concentration of U50488H positively shifted the half-activation membrane potential of I-A by +11 mV, but negatively shifted I-K by -14 mV. These results demonstrate that U50488H can directly inhibit neuronal I-A and I-K without involvement of the activation of kappa -opioid receptors. (C) 2001 Elsevier Science B.V. All rights ...
Samsung CAD300MBEC nab je ka do auta pro C170, D520 a dal ern (EU blister) od v robce SAMSUNG m me skladem jen za 99 K . Osobn odb r nebo dod n do 24 hodin kdekoli v R. Nakupte od specialist na mobiln telefony.
He will feel discontent and extended with himself, complain more than usual, and he essay have a laziness, emotional, physical, and spiritual collapse in a way Colicin v synthesis protein shakes. A lazy person tends to delay things or have someone else do the work, thinking that he cannot do it on his own and that his essay is inferior among the others knowledge, and be extended unfit.
Our service is a large database of words. You can use our database for the selection of a name for their company website, and the like. Also, you learn the value of the most unusual words
1 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATAAC TACGTCAGGT 51 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 101 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 151 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 201 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 251 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 301 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 351 CGCCCCGAAG AACGTTCTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 401 TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 451 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 501 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 551 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 601 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 651 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 701 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 751 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 801 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 851 ...
The time has finally come, ive been planning this cycle for a while now and its about go down. Cycle: (weeks 1-8) Test E: 500/250/0/0/... Msten: 14/14/
Looking for pantothenyl alcohol? Find out information about pantothenyl alcohol. any of a class of organic compounds with the general formula R-OH, where R represents an alkyl group made up of carbon and hydrogen in various proportions... Explanation of pantothenyl alcohol
Plasmid ColIb-P9 (Incll) encodes mechanisms which allow it to avoid destruction by type I and type II restriction enzymes during transfer by conjugation between strains of Escherichia coli. A genetic system was developed to analyse these mechanisms. The system relied on measuring Collb-mediated rescue of the restriction-sensitive plasmid R751 (IncPp) from destruction by EcoKI (type I) and EcoRI (type II). One Collb mechanism was known to involve a plasmid-encoded antirestriction gene known as ardA, the product of which is active against type I enzymes. Tests for alleviation of EcoKI restriction of R751, showed strong protection by a co-transferring Collb (Ard+) plasmid, slight protection when Collb was resident in the recipient and no effect when Collb was immobilised in the donor by removal of its nic site. Hence, expression of ardA is activated in the recipient cell following transfer no detectable transfer of the ArdA protein occurs from the donor to the recipient. The ardA gene is found in ...
Kilómetro 658 (Río Primero, Córdoba, Argentina) with population statistics, charts, map, location, weather and web information.
This work has studied colicin translocation by using a periplasmic protection assay combined with an in vivo lux-reporter assay and a potassium release assay. Expressing the translocation domain of colicin A in the periplasm and challenging the cells with external colicins showed that the translocation of group A colicins is inhibited as it requires an interaction with the Tol system. Surprisingly, the TolA protein was found to play the major role during the translocation of both ColA and the endonuclease colicin E9 even though the latter colicin has no direct interaction with the TolA protein. This study also suggests that the interaction with TolB is important for both colicins A and E9. Moreover, a series of ColA constructs with a truncated T domain were made by site directed mutagenesis to define the important residues of the TolA and TolB boxes for their interaction with Tol proteins. The results showed that tyrosine 58 residue of the TolA box of colicin A is essential for TolA binding, ...
pRV500 is, to our knowledge, the first plasmid of L. sakei to be entirely sequenced. Careful sequence analysis showed that pRV500 belongs to the pUCL287 family, itself related to class A theta-type plasmids of the pUCL22 and pSC101 families. Experimental evidence of the theta replication mode was obtained for pUCL287 itself (2). Although the minimum length of the pRV500 oriA region remains to be determined, it is likely to involve the same two kinds of DNA repeats as other pUCL287-type plasmids. The involvement of repetitive sequences of 22 bp (iterons) as a target for binding of the Rep protein was demonstrated for pSBO1 (40), and the upstream 11-bp repeated sequences were observed to be essential for pUCL287 replication (3).. Like other plasmids (14, 49), pRV500 appears to be a composite structure containing DNA segments from different sources. Some ORFs are shared by plasmids from both subfamilies pUCL22 and pUCL287; this applies to orf6 in pRV500 and ORFs of unknown function around the gene ...
Colicin E1 induces the efflux of carboxyfluorescein and calcein from liposomes whose phospholipid composition is similar to that of Escherichia coli. This colicin action takes place at protein-to-liposome ratios and within pH ranges that are physiologically meaningful. Colicin-induced permeability of carboxyfluorescein is not limited to the initial phase of colicin membrane interaction but is sustained thereafter. Colicin E1 requires negatively charged phospholipids in the liposomal membrane in order to bind and induce efflux.
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Linear diagram showing the approximate lengths of whole colicin Ia and the four carboxy-terminal fragments, CT-S, CT-M, CT-L, and CT-XL. The boundaries of the T
IMM Cologne 2020 kicked off the year with a series of interesting modern design concepts in mind. From January 13-19 at the Koelnmesse exhibition center.
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Theunissen TW, van Oosten AL, Castelo-Branco G, Hall J, Smith A, Silva JC. Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions, Current biology : CB. 2010-12-30 Glass E, Meyer F, Gilbert JA, Field D, Hunter S, Kottmann R, Kyrpides N, Sansone S, Schriml L, Sterk P, White O, Wooley J. Meeting Report from the Genomic Standards Consortium (GSC) Workshop 10, Standards in genomic sciences. 2010-12-25 Johnson TJ, Thorsness JL, Anderson CP, Lynne AM, Foley SL, Han J, Fricke WF, McDermott PF, White DG, Khatri M, Stell AL, Flores C, Singer RS. Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky, PloS one. 2010-12-22 Marques SM, Petushkov VN, Rodionova NS, da Silva JC. LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota, Journal of photochemistry and photobiology. B, Biology. 2010-12-21 Santos MJ, Fernandes D, Capela S, da Silva JC, ...
The segregational stability of bacterial, low-copy-number plasmids is promoted primarily by active partition. The plasmid-specified components of the prototypical P1 plasmid partition system consist of two proteins, ParA (44.3 kDa) and ParB (38.5 kDa), which, in conjunction with integration host fac …
The Role of Bacteriocins in Mediating Bacterial Competitive Interactions. Explaining the coexistence of competing species is a major challenge in community ecology. In bacterial systems, competition is often driven by the production of bacteriocins, which are narrow-spectrum proteinaceous toxins that serve to kill closely related species, providing the producer better access to limited resources. Bacteriocin producers have been shown to competitively exclude sensitive, nonproducing strains. However, the dynamics between bacteriocin producers, each lethal to its competitor, are largely unknown. In this study, we used in vitro, in vivo and in silico models to study competitive interactions between bacteriocin producers. Two Escherichia coli strains were generated, each carrying a DNA-degrading bacteriocin (colicins E2 and E7). Using reporter-gene assays, we showed that each DNase bacteriocin is not only lethal to its opponent but, at lower doses, can also induce the expression of its opponents ...
Individual bacterial cells may contain several different types of plasmids and in some cases more than 10 at a time. Plasmids are generally isolated from the bacterial cells in the supercoiled configuration. So far, thousands of different types of plasmids have been isolated. More than 300 different types of naturally occurring plasmids have been isolated from E.coli alone. Though, plasmids are not considered as part of the cells genome, when a bacterial cell divides each daughter cells receives a copy of each plasmid. Plasmids can also be transferred from one bacterial cell to another by the process called conjugation. Plasmids that govern their own transfer by conjugation are called conjugative plasmids but not all plasmids are conjugative.. ...
All Plasmids Achievement in BioShock 2 (RU) (PC): Found or purchased all 11 basic Plasmid types - worth 20 GamerScore. Find guides to this achievement here.
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Plasmids can be submitted in either form. For DNA, aliquot 15 µL of DNA into a 1.5 mL microfuge tube (at a concentration of 0.1 -1µg/µL)...
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Günther, A., 1880. Report on the shore fishes procured during the voyage of H. M. S. Challenger in the years 1873-1876. A Report on the scientific results of the voyage of H. M. S. Challenger during the years 1873-1876. Zoology. Rept. Challenger Shore Fishes v. 1 (pt 6): 1-82, Pls. 1-32. ...
Definition : Molecular assay reagents intended to identify mutations in the tumor protein p53 (TP53) gene, located at chromosome 17p13.1, which encodes for a protein that acts as a tumor suppressor and induces cellular apoptosis. This inherited genetic mutation and/or genetic anomalies have been identified in patients with many types of cancers.. Entry Terms : 6-Mercaptopurine Inactivation Gene Mutation Reagents , Thiopurine Methyltransferase High Activity Gene Mutation Reagents , TPMT Gene Mutation Detection Reagents , Reagents, Molecular Assay, Gene Anomaly, Mutation, TPMT. UMDC code : 24995 ...
chains in the Genus database with same CATH superfamily 5CCH A; 4WY4 A; 1URQ D; 5KJ7 C; 3HD7 B; 5KJ7 D; 4W80 A; 3RK3 B; 2N1T C; 3HD7 D; 3RK3 C; 2YMY A; 2M8R A; 3RK3 D; 1SFC A; 4W7Y A; 2HFE D; 1GL2 B; 1L4A D; 1SFC B; 2KOG A; 1GL2 C; 1GL2 D; 1SFC C; 1SFC D; 2N1T B; 1N7S A; 1JTH B; 3M0D C; 2N1T D; 1NHL A; 4WY4 B; 3KYQ A; 3B5N A; 3VOP A; 5CCH C; 4WY4 C; 2XDJ A; 5CCH D; 4WY4 D; 1N7S C; 3B5N B; 2PP6 A; 3RL0 B; 3PP5 A; 3B5N C; 3RL0 C; 3B5N D; 3RL0 D; 3TSI A; 1KIL A; 5CCG A; 4OH8 B; 1URQ A; 5KJ7 A; 1N7S B; 3RK2 C; 2NPS A; 1KIL C; 2HG5 D; 3HD7 A; 5CCG C; 1N7S D; 1URQ C; 3P8C E; 2NPS B; 2WZ7 A; 2H8P D; 1L4A A; 2NPS C; 2NPS D; 3HD7 C; 3HTK B; 1GL2 A; 3RK2 B; 4N78 E; 1KIL B; 1L4A B; 3RK2 D; 4W7Z A; 1L4A C; 2N1T A; 4LGD E; 1KIL D; 1JTH A; 1T3J A; 4JF7 A; 1URQ B; 5CCG D; 1HVV A; 1XTG B; #chains in the Genus database with same CATH topology 1KTM A; 1Q86 Q; 4I1L A; 1RF1 C; 1EZV D; 3AE9 C; 3BAT A; 2QSI A; 3V5B A; 3A0H E; 3A3Y B; 3AE1 C; 1HBW A; 5AVS B; 3TYY A; 1SQP K; 1YHQ P; 2XV5 A; 2G3A A; 1KQS O; 1NO4 A; 4JQ0 ...
chains in the Genus database with same CATH superfamily 4WY4 D; 2HG5 D; 5KJ7 C; 4W7Z A; 3RL0 C; 3RK3 C; 5CCH A; 2PP6 A; 1SFC C; 3M0D C; 1JTH B; 3KYQ A; 3RK2 B; 1GL2 B; 3VOP A; 2M8R A; 2N1T A; 5CCG D; 3RL0 B; 5CCH D; 1N7S A; 1L4A C; 3RK2 D; 3B5N D; 2WZ7 A; 1GL2 C; 3HD7 A; 1N7S B; 1URQ A; 2YMY A; 1KIL C; 3B5N B; 1GL2 D; 5CCH C; 2NPS A; 1SFC A; 3TSI A; 1L4A D; 3RL0 D; 3B5N A; 5CCG C; 1NHL A; 4WY4 A; 1N7S D; 3PP5 A; 4WY4 C; 1L4A A; 1URQ C; 2HFE D; 1L4A B; 1JTH A; 5KJ7 A; 2N1T B; 1T3J A; 1KIL D; 2XDJ A; 2KOG A; 3P8C E; 1HVV A; 2N1T D; 2NPS D; 3HD7 B; 1GL2 A; 4W7Y A; 2N1T C; 4LGD E; 4OH8 B; 3RK3 B; 1KIL A; 3HD7 D; 2NPS B; 1SFC B; 3HD7 C; 4JF7 A; 3RK2 C; 3HTK B; 4W80 A; 1KIL B; 3B5N C; 5CCG A; 1SFC D; 1URQ B; 2NPS C; 1XTG B; 3RK3 D; 1URQ D; 4N78 E; 1N7S C; 4WY4 B; 5KJ7 D; 2H8P D; #chains in the Genus database with same CATH topology 1RF1 C; 1SQP D; 2L91 A; 2Y9Y A; 5AW2 B; 2HG5 D; 5KJ7 C; 1M1J B; 1YQ3 C; 2XNX A; 3L70 D; 1OWA A; 2KI7 B; 2IZ0 A; 1AQT A; 4GOF A; 2KXP B; 2R5D A; 4H44 B; 4JQ0 D; 3KQG A; 3MZW ...
I grew up being taught that no self-respecting potter would ever pronounce the n in kiln but would say it as kil. I ve since run into potters -- amongst many others -- who do say the n . Today I happened to wander by a TV with Antiques Roadshow on and overheard the appraiser use the kil pronunciation -- the first time in years that I ve heard it said correctly. ...
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CORTU : Preferred screening test for Cushing syndrome   Diagnosis of pseudo-hyperaldosteronism due to excessive licorice consumption   Test may not be useful in the evaluation of adrenal insufficiency
Binding of enzymatic E colicins to the vitamin B12 receptor, BtuB, is the first stage in a cascade of events that culminate in the translocation of the cytotoxic nuclease into the Escherichia coli cytoplasm and release of its tightly bound immunity protein. A dogma of colicin biology is that the toxin coiled-coil connecting its functional domains must unfold or unfurl to span the periplasm, with recent reports claiming this reaction is initiated by receptor binding. We report isothermal titration calorimetry data of BtuB binding the endonuclease toxin ColE9 and a disulfide form (ColE9S-S) where unfolding of the coiled-coil is prevented and, as a consequence, the toxin is biologically inactive. Contrary to expectation, the thermodynamics of receptor binding, characterized by large negative values for TDeltaS, are identical for the two colicins, arguing against any form of BtuB-induced unfolding. We go on to delineate key features of the colicin translocon that assembles at the cell surface after BtuB
SimPlot analysis.Similarity plots with plasmids R751, pBP136 and pB3 as reference plasmids. Each coloured plot corresponds to a specific plasmid depicted in the
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Supplementary MaterialsSupplementary materials 1 mgen-6-353-s001. mix of brief and long-read entire genome sequencing strategies, we were able to assemble total sequences of 44 plasmids, with 16 Inc group F and 20 col plasmids; antibiotic resistance genes located almost specifically within the F group. gene encoding resistance to trimethoprim, PF 429242 tyrosianse inhibitor therefore linking trimethoprim resistance to the additional antibiotic resistance genes within the plasmids. This will allow even narrow spectrum antibiotics such as trimethoprim to act Mouse monoclonal to CD95(PE) like a selective agent for plasmids comprising antibiotic resistance genes mediating much broader resistance, including expressing prolonged spectrum -lactamases (ESBL) which produce resistance to 3rd generation cephalosporins - in England in 2017 13?% of bloodstream isolates of were resistant to 3rd generation cephalosporins [10], while within Europe the pace was 14.9?% [11]. Related rates are reported from the ...
The SCOP classification for the Colicin E3 immunity protein superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
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Please send us 10-50ul of samples with the concentration of 50-100ng/μl.Please do not send us your plasmids in low volume since they tend to evaporate.. ...
Bacteriocin genes are located either on chromosomes or on plasmids. Strain 5 produces a plasmid-encoded bacteriocin that is ... A Theta-type plasmid has been characterized in Lactobacillus sakei in 2003. It is a potential basis for Low-Copy-Number vectors ... Sakacins are bacteriocins of class II produced by L. sakei. In strain CCUG 42687, their production is dependent on nutrients, ... The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and ...
Association of a 13.6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl. Environ. Microbiol. 50: ... Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12 :39S-85S Barros R.R., Carvalho G.S., Peralta ... Anti-Listeria effect of enoterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins ... primarily through the production of lactic acid and secretion of bacteriocins known as pediocins. P. acidilactici has a wide ...
Biotyping, bacteriocin typing, phage typing, plasmid analysis, and ribotyping can also be used. Most strains of S. marcescens ...
Kerry-Williams, S.M.; Noble, W.C. (1984). "Plasmid-associated bacteriocin production in a JK-type coryneform bacterium". FEMS ... Kerry-Williams, S. M.; Noble, W. C. (2009). "Plasmids in group JK coryneform bacteria isolated in a single hospital". Journal ... Some species produce metabolites similar to antibiotics: bacteriocins of the corynecin-linocin type, antitumor agents, etc. One ... Kono, M.; Sasatsu, M.; Aoki, T. (1983). "R Plasmids in Corynebacterium xerosis Strains". Antimicrobial Agents and Chemotherapy ...
Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. Degradative plasmids, ... Plasmids can be broadly classified into conjugative plasmids and non-conjugative plasmids. Conjugative plasmids contain a set ... Many plasmids have been created over the years and researchers have given out plasmids to plasmid databases such as the non- ... Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens Plasmids can belong ...
"Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13". Nucleic Acids Res. 11 (8 ... The two general classes of colicinogenic plasmids are large, low-copy-number plasmids, and small, high-copy-number plasmids. ... Retaining the colicin plasmid is very important for cells that live with their relatives, because if a cell loses the immunity ... The larger plasmids carry other genes, as well as the colicin operon. The colicin operons are generally organized with several ...
... s, also known as bacteriocinogenic plasmids, are bacterial plasmids that direct the synthesis of bacteriocins, ... Normally the bacteriocinogen is repressed and doesn't expresss bacteriocin, but under certain conditions the plasmid is ... Wiley,USA v t e (Orphaned articles from August 2015, All orphaned articles, Plasmids, All stub articles, Microbiology stubs). ... "In vitro construction of deletion mutants of the bacteriocinogenic plasmid Clo DF13". Nucleic Acids Research. 5 (6): 1801-20. ...
Bacteriocin AS-48 is encoded by the pheromone-responsive plasmid pMB2, and acts on the plasma membrane in which it opens pores ... though it lacks sequence homology with bacteriocins AS-48. Bacteriocin uses components of the mannose phosphotransferase system ... Bacteriocin AS-48 is a cyclic peptide antibiotic produced by the eubacteria Enterococcus faecalis (Streptococcus faecalis) that ... González C, Langdon GM, Bruix M, Gálvez A, Valdivia E, Maqueda M, Rico M (October 2000). "Bacteriocin AS-48, a microbial cyclic ...
Bacteriocin AS-48 is encoded by the pheromone-responsive plasmid pMB2, and acts on the plasma membrane in which it opens pores ... Bacteriocins for which disulfide bonds are the only modification to the peptide are Class II bacteriocins. One important and ... The class IIb bacteriocins (two-peptide bacteriocins) require two different peptides for activity. It includes the alpha ... Class II bacteriocins are a class of small peptides that inhibit the growth of various bacteria. Many Gram-positive bacteria ...
... large plasmids, small plasmids, chromosomal), molecular weight and chemistry (large protein, peptide, with/without sugar moiety ... Colicins are bacteriocins found in the Gram-negative E. coli. Similar bacteriocins (CLBs, colicin-like bacteriocins) occur in ... Wikimedia Commons has media related to Bacteriocin. Bagel Bacteriocin Database BACTIBASE Database Bacteriocins at the US ... They are the longest studied bacteriocins. They are a diverse group of bacteriocins and do not include all the bacteriocins ...
This sample did not contain any plasmids. The most extensively studied strain, L. rhamnosus GG, a gut isolate, consists of a ... bacteriocin production, pili production, the CRISPR-Cas system, the clustered regularly interspaced short palindromic repeat ( ... CRISPR) loci, and more than 100 transporter functions and mobile genetic elements such as phages, plasmid genes, and ...
... and Plasmid Profiles". Phytopathology. 88 (11): 1179-86. doi:10.1094/phyto.1998.88.11.1179. PMID 18944851. Bouarab, edited by ... Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene". Applied and ... Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene". Applied and ...
The two plasmids are pTiC58, responsible for the processes involved in virulence, and pAtC58, once dubbed the "cryptic" plasmid ... K84 produces a bacteriocin (agrocin 84) which is an antibiotic specific against related bacteria, including A. tumefaciens. ... To be virulent, the bacterium contains tumour-inducing plasmid (Ti plasmid or pTi), of 200 kbp, which contains the T-DNA and ... PMID 32915125.{{cite journal}}: CS1 maint: uses authors parameter (link) "At plasmid" when talking about related plasmids Smith ...
Many lactobacilli also contain multiple plasmids. A recent study has revealed that plasmids encode the genes which are required ... The antibacterial and antifungal activity of lactobacilli relies on production of bacteriocins and low molecular weight ... Davray D, Deo D, Kulkarni R (November 2020). "Plasmids encode niche-specific traits in Lactobacillaceae". Microbial Genomics. 7 ... Lactobacilli produce bacteriocins to suppress pathogenic growth of certain bacteria, as well as lactic acid and H2O2 (hydrogen ...
This genome has one circular chromosome and two plasmids. The two plasmids found in the genome are pGD01 and pGD02 which ... produces a bacteriocin against Xanthomonas albilineans, a sugar cane pathogen". Research in Microbiology. 153 (6): 345-351. doi ...
Plasmid pBCE4810 shares homology with the B. anthracis virulence plasmid pXO1, which encodes the anthrax toxin. Periodontal ... Naclerio G, Ricca E, Sacco M, De Felice M (December 1993). "Antimicrobial activity of a newly identified bacteriocin of ... structure and location on a mega virulence plasmid related to Bacillus anthracis toxin plasmid pXO1". BMC Microbiology. 6: 20. ... It was shown independently by two research groups to be encoded on multiple plasmids: pCERE01 or pBCE4810. ...
These conjugative plasmids carry a number of genes that can be assembled and rearranged, which could then enable bacteria to ... Another research team was able to use bacteriocins, antimicrobial peptides and bacteriophages in the control of bacterial ... Bennett P. M. (2008). Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in ... Joerger R.D. (2003). "Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages". Poultry Science. ...
Genes for bacteriocins may reside on plasmids.. Read more about this term in the Online Textbook:. Emerging Infectious Diseases ... Bacteriocin. Proteins produced by some bacteria, which inhibit the growth of other strains of the same organism or related ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ...
Genes for bacteriocins may reside on plasmids.. Read more about this term in the Online Textbook:. Emerging Infectious Diseases ... Bacteriocin. Proteins produced by some bacteria, which inhibit the growth of other strains of the same organism or related ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ...
Potential Markets for Bacteriocins as Food Antibacterials. Bacteriocins such as colicins and salmocins are promising ... Plasmid Constructs. Constructs used were described in Schulz et al. (2015) or Schneider et al. (2018). ... Table 1. Bacteriocins versus antibiotics: major biological differences and market potential of bacteriocins. ... 2018). Plant-made Salmonella bacteriocins salmocins for control of Salmonella pathovars. Sci. Rep. 8:4078. doi: 10.1038/s41598- ...
Association of a 13.6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl. Environ. Microbiol. 50: ...
Research field: Plasmid biology and molecular biology of acidophilic biomining bacteria, microbial interactions, bacteriocin ...
Bacteriocins, a heterogeneous group of bioactive bacterial peptides or proteins, are ribosomally synthesized that inhibit or ... Genes encoding these bacteriocins can be present either on the plasmid or chromosome as a subset of three genes, bacteriocin ... Classification of Bacteriocins. Bacteriocins are classified into different groups (Table 2). Class I bacteriocins (lantibiotics ... Table 6: Potential applications of bacteriocin in various formulations sector.. Bacteriocin Toxicity. Bacteriocins have been ...
The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut ... Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L ... N2 - The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal ... AB - The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal ...
Plasmid-determined Bacteriocin Production by Rhizobium leguminosarum. A - Papers appearing in refereed journals ... Conjugative plasmids in Rhizobium. Abstracts ESF Workshop on Plasmid Mediated Gene Transfer, University of Birmingham, 12-15 ... Ecology of plasmid transfer and spread. in: Thomas, C. M. (ed.) The horizontal gene pool: bacterial plasmids and gene spread ... Abstracts 3rd ESF Workshop on Molecular Biology and Ecology of Plasmid-Mediated Gene Transfer, Cuenca, 12-16 September 1997 . ...
Bacteriocin Plasmids. en_US. dc.subject.mesh. Colicins --biosynthesis. en_US. dc.subject.mesh. Drug Resistance, Microbial. en_ ... Sharma PL, Sharma KB, Prakash K. Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. Indian Journal ... Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae.. en_US. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
Bacteriocin Plasmids. M - PERSONS. Changed terms. Replaced-by. M01 - PERSONS. Blacks. African Americans. ...
A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ...
Keywords: Lactobacillus plantarum, Bacteriocin, plasmid, UV induction , pages 1488-1504 , abstract and details , fulltext pdf ...
Factor, Col use Bacteriocin Plasmids Factor, Colicin use Bacteriocin Plasmids Factor, Confounding (Epidemiology) use ... Factors, Col use Bacteriocin Plasmids Factors, Colicin use Bacteriocin Plasmids Factors, Confounding (Epidemiology) use ...
Distinct bacteriocin groups correlate with different groups of Streptococcus mutans plasmids. Infection Immunity. 1985;48(1):51 ...
  • Three LAB strains (firstly named as Lactobacillus curvatus 12, L. curvatus 36 and Weissella viridescens 23) were obtained from calabresa by presenting promising bacteriocinogenic activity, distinct genetic profiles (rep-PCR, RAPD, bacteriocin-related genes) and wide inhibitory spectrum. (biomedcentral.com)
  • Plasmids are, however, much smaller than the bacterial chromosome, usually only containing a few genes. (scienceprofonline.com)
  • Plasmids called R factors carry genes for antibiotic resistance, or resistance to chemicals that, without the plasmid, would be toxic to the bacterium. (scienceprofonline.com)
  • Bacteriocin factors carry genes for making proteins that kill other bacteria that do not have this factor, reducing competition for the bacteria that have it. (scienceprofonline.com)
  • Other plasmids, called virulence factors have genes that help bacteria cause disease, such as the productions exotoxins that make those infected with the bacterium ill, or genes for adhesins, that allow the bacterium to adhere to and infect certain host cell types. (scienceprofonline.com)
  • The predicted protein-coding genes, which are encoding the CRISPR-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. (ejast.org)
  • These include a large number of genes that code for secreted toxins and enzymes, as well as genes that encode products for the production of antibiotics and bacteriocins. (up.ac.za)
  • The observed distribution of resistance plasmids and β-lactamase genes in several clones indicates a high degree of horizontal transfer. (cdc.gov)
  • Analysis of the 5.04 MB chromosomal genome predicts 4599 protein coding genes, seven sets of ribosomal RNA genes, 84 tRNA genes and a 64.8 KB plasmid encoding 74 genes. (uncg.edu)
  • SCBI (South African Caenorhabditis briggsae Isolate) are found in ~29 genomic islands of 5 to 65 genes and are enriched in putative functions that are biologically relevant to an entomopathogenic lifestyle, including non-ribosomal peptide synthetases, bacteriocins, fimbrial biogenesis, ushering proteins, toxins, secondary metabolite secretion and multiple drug resistance/efflux systems. (uncg.edu)
  • Multiple plasmid-borne virulence genes of Clavibacter michiganensis ssp. (uni-bielefeld.de)
  • Sequencing and analysis of the ( pla ) gene, located on plasmid pPCP1, the answers. (cdc.gov)
  • The plasminogen activator/coagulase ( pla ) gene, located on plasmid pPCP1, is incorporated into most Y. pestis PCR assays, and in several studies it was the prime or sole marker ( 1 , 2 , 5 , 7 - 9 ). (cdc.gov)
  • Microbial production of bacteriocins and its applications in food preservation: A review. (alliedacademies.org)
  • Garai B, Purkait A, Ghose T ,et al.Microbial production of bacteriocins and its applications in food preservation: A review. (alliedacademies.org)
  • Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6. (hammamilab.org)
  • The type III system is closely related to the Yersinia plasmid-encoded type III system. (up.ac.za)
  • We hypothesize the putative bacteriocin, transposase, and N-acetylmuramoyl-L-alanine amidase, based on their DNA sequences, are involved in lysis. (emerging-researchers.org)
  • Bacteriocins are a large family of ribosomally synthesized proteinaceous toxins produced by bacteria and Archaea that have antimicrobial activity against bacteria closely related to the producer strain [ 9 ]. (alliedacademies.org)
  • Bacteriocins are protein compounds that present a variable spectrum of antimicrobial activity, usually against closely related species to the producer strains [ 3 ]. (biomedcentral.com)
  • Although plasmids are most often exchanged between closely related bacteria, some plasmids can be shared by very dissimilar microbes. (scienceprofonline.com)
  • Bacteriocins produced by lactic acid bacteria (LAB) can be considered as viable alternatives for food safety and quality, once these peptides present antimicrobial activity against foodborne pathogens and spoilage bacteria. (biomedcentral.com)
  • However, only six isolates among which E. faecalis 14, E. faecalis 28, E. faecalis 90, E. faecalis 97, and E. faecalis 101 (obtained from donor 3), and E. faecalis 93 (obtained from donor 5) were active against some Gram-negative bacteria and Gram-positive bacteria, through production of lactic acid, and bacteriocin like inhibitory substances. (cyberleninka.org)
  • Partially purified bacteriocins from L. curvatus 12 kept their inhibitory activity after elution with isopropanol at 60% ( v /v). Bacteriocins produced by this strain were purified by HPLC and sequenced, resulting in four peptides with 3102.79, 2631.40, 1967.06 and 2588.31 Da, without homology to known bacteriocins. (biomedcentral.com)
  • Among these isolates, bacteriocins produced by L. curvatus 12, now named as L. curvatus UFV-NPAC1, presented the highest inhibitory performance and the purification procedures revealed four peptides with sequences not described for bacteriocins to date. (biomedcentral.com)
  • Genetic determinants of lactose fermentation, proteolytic activity and bacteriocin production in the strains L. lactis subsp. (sdu.edu.tr)
  • As a result of mutation and genetic transfer studies, it was determined that ail of these characters were encoded by the 65.4 kb plasmid in L. lactis subsp. (sdu.edu.tr)
  • In order to transfer a plasmid, the bacterium with the plasmid must have the genetic instructions required to construct a sex pilus-a prokaryotic cell extension that can hook up to a recipient bacterium, connecting the two bacteria and physically pulling them towards each other. (scienceprofonline.com)
  • Genetic structure of the Enterococcus faecalis plasmid pAD1-encoded cytolytic toxin system and its relationship to lantibiotic determinants. (researchsnappy.com)
  • a Genetic locus producing the antimicrobial activity and alignment with homologous loci found on plasmid pBac115 of an S. epidermidis strain and on the chromosome of B. cereus ATCC14579, described to produce the thiopeptide micrococcin P1. (biomedcentral.com)
  • Bacteriocins, a heterogeneous group of bioactive bacterial peptides or proteins, are ribosomally synthesized that inhibit or kill other related or unrelated microorganisms. (alliedacademies.org)
  • A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. (elsevier.com)
  • The plasmid insertion conferring the antilisterial-peptide- negative phenotype was located in a seven-gene operon (alb, for antilisterial bacteriocin) residing immediately downstream from the sbo gene, which encodes the precursor of subtilosin. (elsevier.com)
  • To investigate whether the presence of the pla gene sequences indicated the presence of the carrier pPCP1 plasmid in Y. pestis , we designed PCR assays for the amplification of 3 conserved regions of this plasmid ( Technical Appendix ). (cdc.gov)
  • Inducible rescue plasmids containing functional gene inserts will be transformed into knockout P. larvae cultures to restore the wildtype phenotype. (emerging-researchers.org)
  • A TaqMan real-time PCR for identification of B. anthracis was developed, based on the two plasmids, pX01 and pX02, both of which are necessary for pathogenicity, as well as on the chromosomally encoded rpoB gene, to confirm or exclude potential attacks approximately 2-3 h after the material has arrived in the laboratory. (semanticscholar.org)
  • Multiple PCR assays for Y. pestis detection that primarily detect markers located on plasmids have been developed ( 1 - 6 ). (cdc.gov)
  • In 2011, Germany experienced the largest outbreak with a Shiga toxin-producing Escherichia coli (STEC) strain ever recorded, and researchers explored bacteriophages, bacteriocins, and low-molecular-weight inhibitors against STEC. (semanticscholar.org)
  • Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods. (dtu.dk)
  • Fermented foods, such as artisanal sausages and cured meats, are relevant sources of LAB strains capable of producing novel bacteriocins, with particular interest by the food industry. (biomedcentral.com)
  • Among these strains, L. curvatus 12 presented higher bacteriocin production, reaching 25,000 AU/mL after incubation at 25, 30 and 37 °C and 6, 9 and 12 h. (biomedcentral.com)
  • Thus, the present study aimed to isolate bacteriocinogenic LAB strains from artisanal meats produced in Minas Gerais state, Brazil, and to characterize their produced bacteriocins towards their antimicrobial features and structure. (biomedcentral.com)
  • Can they increase the colonizing capability of plasmid carrying bacteria? (antimicrobialresistance.eu)
  • Numerous bacteriocins produced by LAB species have been already described and they are well known by their activity against spoilage bacteria and foodborne pathogens [ 3 ]. (biomedcentral.com)
  • diacetylactis MPD166 strain were found to be responsible for the 61.0 kb, 14.2 and 9.0 kb plasmids respectively. (sdu.edu.tr)
  • The complete genome sequences of the strain contained one circular chromosome (2,057,809 bp) with 38.2% GC content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. (ejast.org)
  • IMSEAR at SEARO: Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. (who.int)
  • Sharma PL, Sharma KB, Prakash K. Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. (who.int)
  • Lactose fermentation and proteolytic activity were encoded by the 68.0 kb plasmid and bacteriocin production was encoded by the 12.4 kb plasmid in L. lactis subsp. (sdu.edu.tr)
  • By electron microscopy, restriction analysis and DNA sequencing, it was demonstrated that the phage and the plasmid DNAs are linear, circularly permuted molecules. (semanticscholar.org)
  • A plasmid is a molecule of DNA, independent of the bacterial nucleoid chromosome, which often has 'bonus DNA' with instructions for weapons of infection. (scienceprofonline.com)
  • During this period Photorhabdus luminescens releases bacteriocidal products, including antibiotics and bacteriocins, that prevent infection of the larva by competitive microbes. (up.ac.za)
  • The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. (dtu.dk)
  • Pediocin PA-1 did not mediate changes of the rat microbiota, and a biological assay indicated that the bacteriocin was degraded or inactivated during passage through the intestine. (dtu.dk)
  • Comparison of the presubtilosin and mature subtilosin sequences suggested that certain residues undergo unusual posttranslational modifications unlike those occurring during the synthesis of class I (lantibiotic) or some class II bacteriocins. (elsevier.com)
  • A donor and a recipient do not always need to be of the same species in order to share a plasmid. (scienceprofonline.com)
  • A plasmid that can replicate in one, or at most a few, different bacterial species. (expertglossary.com)
  • Unlike the bacterial chromosome, plasmid traits are not normally required for the cell to function, but do help a bacterium survive and cause disease. (scienceprofonline.com)
  • There are many different types of plasmids, in addition to F factors mentioned above. (scienceprofonline.com)
  • Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. (elsevier.com)
  • Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis. (hammamilab.org)
  • Since they pose no health risk concerns, soon bacteriocins are largely dependent on their use as safe food preservatives. (alliedacademies.org)
  • Besides, this article stated the potential applications of bacteriocin in the bactericidal formulations sector. (alliedacademies.org)