Bacteriocins
Plasmids
Pediococcus
Lactobacillus
Conjugation, Genetic
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Listeria
Nisin
Lactococcus lactis
Lactococcus
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Base Sequence
R Factors
Lactobacillaceae
Enterococcus faecalis
Lactobacillus acidophilus
Amino Acid Sequence
Enterococcus faecium
Transformation, Bacterial
Gram-Positive Asporogenous Rods
Propionibacterium
A genus of gram-positive, rod-shaped bacteria whose cells occur singly, in pairs or short chains, in V or Y configurations, or in clumps resembling letters of the Chinese alphabet. Its organisms are found in cheese and dairy products as well as on human skin and can occasionally cause soft tissue infections.
Serratia marcescens
Extrachromosomal Inheritance
Cloacin
Cloning, Molecular
Pyocins
Mitomycins
Sequence Analysis, DNA
Colicins
Drug Resistance, Microbial
Leuconostoc
Streptococcus mutans
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Food Microbiology
Operon
Streptococcus
Antibiosis
Listeria monocytogenes
Gene Expression Regulation, Bacterial
Open Reading Frames
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Microbial Sensitivity Tests
Lactobacillus plantarum
Brevibacillus
Cheese
Restriction Mapping
Chromosomes, Bacterial
Pectobacterium carotovorum
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Mutation
Fermentation
Transformation, Genetic
Megacins
Enterococcus
Replicon
DNA, Recombinant
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Isolation and characterization of ColE1-derived plasmid copy-number mutant. (1/196)
The plasmid pBGP120 is a ColE1 derivative that contains elements of the Escherichia coli lac operon and the Tn3 transposon. We have selected and isolated a copy-number mutant of pBGP120. In exponentially growing cultures, the copy-number mutant, pOP1, represents approximately 30% of total intracellular DNA compared to about 5% for pBGP120. Plasmid-encoded beta-galactosidase monomer can represent 50% of newly synthesized protein in cells carrying pOP1. pOP1 is structurally unstable in certain genetic backgrounds and under certain growth conditions, breaking down to a smaller sized plasmid that retains the DNA overproducer phenotype and the Tn3 transposon. The smaller overproducer plasmid, pOP1delta6, is generated by a continuous deletion of sequences located between one end of the Tn3 transposon and a site about 630 nucleotides from the EcoRI site in the beta-galactosidase structural gene of pOP1. pOP1delta6 retains the ColE1 origin of replication but has lost the lac promotor and operator and most of the beta-galactosidase structural gene. pOP1delta6 exists at approximately 210 copies per chromosome in exponentially growing cells. (+info)Analysis of the CoIE1 stability determinant Rcd. (2/196)
Multimer formation is an important cause of instability for many multicopy plasmids. Plasmid CoIE1 is maintained stably because multimers are converted to monomers by Xer-mediated site-specific recombination at the cer site. However, multimer resolution is not the whole story; inactivation of a promoter (Pcer) within cer causes plasmid instability even though recombination is unaffected. The promoter directs the synthesis of a short transcript (Rcd) which is proposed to delay the division of multimer-containing cells. Mapping of the 5' terminus of Rcd confirms that transcription initiates from Pcer. The 3' terminus shows considerable heterogeneity, consistent with a primary transcript of 95 nt being degraded via intermediates of 79 and 70 nt. Secondary structure predictions for Rcd are presented. Of four mutations which abolish Rcd-mediated growth inhibition, one reduces the activity of Pcer while the other three map to the rcd coding sequence and reduce the steady-state level of the transcript. RNA folding analysis suggests that these three mutant transcripts adopt a common secondary structure in which the major stem-loop differs from that of wild-type Rcd. A survey of 24 cer-like multimer resolution sites revealed six which contain Pcer-like sequences. The putative transcripts from these sites have similar predicted secondary structures to Rcd and contain a highly conserved 15 base sequence. To test the hypothesis that Rcd acts as an anti-sense RNA, interacting with its target gene(s) through the 15 nt sequence, we used DNA hybridization and sequence analysis to find matches to this sequence in the Escherichia coli chromosome. Our failure to find plausible anti-sense targets has led to the suggestion that Rcd may interact directly with a protein target. (+info)Expression of leading region genes on IncI1 plasmid ColIb-P9: genetic evidence for single-stranded DNA transcription. (3/196)
The leading region of a plasmid is the first sector to enter the recipient cell in bacterial conjugation. This sector of IncI1 plasmid ColIb-P9 includes genes that are transcribed in a transient pulse early in the conjugatively infected cell to promote establishment of the immigrant plasmid. Evidence is presented that the burst of gene expression is regulated by a process which is independent of a repressor but dependent on the orientation of the genes on the unique plasmid strand transferred in conjugation. The nucleotide sequence of 11.7 kb of the leading region was determined and found to contain 10 ORFs; all are orientated such that the template strand for transcription corresponds to the transferred strand. The leading region contains three dispersed repeats of a sequence homologous to a novel promoter in ssDNA described by H. Masai & K. Arai (1997, Cell 89, 897-907). It is proposed that the repeats are promoters that form in the transferring strand of ColIb to support transient transcription of genes transferred early in conjugation. (+info)Features of distamycin preferential binding sites on natural DNA predicted using differential scanning calorimetry. (4/196)
The interaction of distamycin with ColE1 DNA was examined by using differential scanning calorimetry (DSC) taking the helix-coil transition theory of DNA into consideration. Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends. (+info)Monomer-dimer control of the ColE1 P(cer) promoter. (5/196)
XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), located centrally within cer, is also required for stable plasmid maintenance. P(cer) is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P(cer) in plasmid monomers. P(cer) is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this study has demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P(cer) activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally. (+info)Purification and characterization of a proteolytic active fragment of DNA topoisomerase I from the brine shrimp Artemia franciscana (Crustacea Anostraca). (6/196)
The ATP-independent type I topoisomerase from the crustacean Artemia franciscana was purified to near-homogeneity. Its activity was measured by an assay that uses the formation of an enzyme-cleaved DNA complex in the presence of the specific inhibitor camptothecin. The purification procedure is reported. Purified topoisomerase is a single-subunit enzyme with a molecular mass of 63 kDa. Immunoblot performed on the different steps of purification shows that the purified 63 kDa peptide is a proteolytic fragment of a protein with a molecular mass of 110 kDa. Similarly to the other purified eukaryotic topoisomerases, the crustacean enzyme does not require a bivalent cation for activity, but is stimulated in the presence of 10 mM-MgCl2; moreover, it can relax both negative and positive superhelical turns. The enzyme activity is strongly inhibited by the antitumour drug camptothecin. The enzyme inhibition is related to the stabilization of the cleavable complex between topoisomerase I and DNA. (+info)Structure of the ColE1 DNA molecule before segregation to daughter molecules. (7/196)
The segregation of daughter DNA molecules at the end stage of replication of plasmid ColE1 was examined. When circular ColE1 DNA replicates in a cell extract at a high KCl concentration (140 mM), a unique class of molecules accumulates. When the molecule is cleaved by a restriction enzyme that cuts the ColE1 DNA at a single site, an X-shaped molecule in which two linear components are held together around the origin of DNA replication is made. For a large fraction of these molecules, the 5' end of the leading strand remains at the origin and the 3' end of the strand is about 30 nucleotides upstream of the origin. The 3' end of the lagging strand is located at the terH site (17 nucleotides upstream of the origin) and the 5' end of the strand is a few hundred nucleotides upstream of the terH site. Thus the parental strands of the molecule intertwine with each other only once. When the KCl concentration is lowered to 70 mM, practically all of these molecules are converted to daughter circular monomers or to catenanes consisting of two singly interlocked circular units. (+info)A new colicin that adsorbs to outer-membrane protein Tsx but is dependent on the tonB instead of the tolQ membrane transport system. (8/196)
A new colicin, Col5, was synthesized by an Escherichia coli isolate of human origin from the ECOR Collection. It was unique because it adsorbed to the outer-membrane protein Tsx, but used the tonB rather than the tolQ membrane transport system, which is employed by the only other Tsx-specific colicin, ColK. Col5 was encoded by a 5.2 kb plasmid, p5. It was inducible by mitomycin C, and strains harbouring p5 exhibited quasi-lysis. The bactericidal protein had an M(r) of 56,000. (+info)
Microbiology Society Journals | Evolution of colicin BM plasmids: the loss of the colicin B activity gene
ColV Plasmid-Mediated, Colicin V-Independent Iron Uptake System of Invasive Strains of Escherichia coli | Infection and Immunity
serine-type endopeptidase activity Gene Ontology Term (GO:0004252)
P-type potassium transmembrane transporter activity Gene Ontology Term (GO:0008556)
High Levels of Colicin Resistance in Escherichia coli | Riley Lab
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Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky...
Controlled secretion into the culture medium of a hybrid beta-glucanase by Acetobacter methanolicus mediated by the kil gene of...
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Investigating the molecular mechanisms of colicin import into Escherichia coli cells - Nottingham ePrints
Characterization of a Theta-Type Plasmid from Lactobacillus sakei: a Potential Basis for Low-Copy-Number Vectors in...
Membrane action of colicin E1: detection by the release of carboxyfluorescein and calcein from liposomes. | Semantic Scholar
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The partition system of multidrug resistance plasmid TP228 includes a novel protein that epitomizes an evolutionarily distinct...
Bacteriocins | Riley Lab
Plasmids: Properties, Types and Functions - Learn Microbiology Online
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Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor binding. - Oxford...
SimPlot analysis.Similarity plots with plasmids R751, p | Open-i
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Colicin E3 immunity protein superfamily
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DNA Prep Frequently Asked Questions
Latilactobacillus sakei
Bacteriocin genes are located either on chromosomes or on plasmids. Strain 5 produces a plasmid-encoded bacteriocin that is ... A Theta-type plasmid has been characterized in Lactobacillus sakei in 2003. It is a potential basis for Low-Copy-Number vectors ... Sakacins are bacteriocins of class II produced by L. sakei. In strain CCUG 42687, their production is dependent on nutrients, ... The key elements of these vectors are a regulatable promoter involved in the production of the bacteriocins sakacin A and ...
Pediococcus acidilactici
Association of a 13.6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl. Environ. Microbiol. 50: ... Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12 :39S-85S Barros R.R., Carvalho G.S., Peralta ... Anti-Listeria effect of enoterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins ... primarily through the production of lactic acid and secretion of bacteriocins known as pediocins. P. acidilactici has a wide ...
Serratia
Biotyping, bacteriocin typing, phage typing, plasmid analysis, and ribotyping can also be used. Most strains of S. marcescens ...
Corynebacterium
Kerry-Williams, S.M.; Noble, W.C. (1984). "Plasmid-associated bacteriocin production in a JK-type coryneform bacterium". FEMS ... Kerry-Williams, S. M.; Noble, W. C. (2009). "Plasmids in group JK coryneform bacteria isolated in a single hospital". Journal ... Some species produce metabolites similar to antibiotics: bacteriocins of the corynecin-linocin type, antitumor agents, etc. One ... Kono, M.; Sasatsu, M.; Aoki, T. (1983). "R Plasmids in Corynebacterium xerosis Strains". Antimicrobial Agents and Chemotherapy ...
Plasmid
Col plasmids, which contain genes that code for bacteriocins, proteins that can kill other bacteria. Degradative plasmids, ... Plasmids can be broadly classified into conjugative plasmids and non-conjugative plasmids. Conjugative plasmids contain a set ... Many plasmids have been created over the years and researchers have given out plasmids to plasmid databases such as the non- ... Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens Plasmids can belong ...
Colicin
"Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid Clo DF13". Nucleic Acids Res. 11 (8 ... The two general classes of colicinogenic plasmids are large, low-copy-number plasmids, and small, high-copy-number plasmids. ... Retaining the colicin plasmid is very important for cells that live with their relatives, because if a cell loses the immunity ... The larger plasmids carry other genes, as well as the colicin operon. The colicin operons are generally organized with several ...
Bacteriocinogen
... s, also known as bacteriocinogenic plasmids, are bacterial plasmids that direct the synthesis of bacteriocins, ... Normally the bacteriocinogen is repressed and doesn't expresss bacteriocin, but under certain conditions the plasmid is ... Wiley,USA v t e (Orphaned articles from August 2015, All orphaned articles, Plasmids, All stub articles, Microbiology stubs). ... "In vitro construction of deletion mutants of the bacteriocinogenic plasmid Clo DF13". Nucleic Acids Research. 5 (6): 1801-20. ...
Bacteriocin IId
Bacteriocin AS-48 is encoded by the pheromone-responsive plasmid pMB2, and acts on the plasma membrane in which it opens pores ... though it lacks sequence homology with bacteriocins AS-48. Bacteriocin uses components of the mannose phosphotransferase system ... Bacteriocin AS-48 is a cyclic peptide antibiotic produced by the eubacteria Enterococcus faecalis (Streptococcus faecalis) that ... González C, Langdon GM, Bruix M, Gálvez A, Valdivia E, Maqueda M, Rico M (October 2000). "Bacteriocin AS-48, a microbial cyclic ...
Class II bacteriocin
Bacteriocin AS-48 is encoded by the pheromone-responsive plasmid pMB2, and acts on the plasma membrane in which it opens pores ... Bacteriocins for which disulfide bonds are the only modification to the peptide are Class II bacteriocins. One important and ... The class IIb bacteriocins (two-peptide bacteriocins) require two different peptides for activity. It includes the alpha ... Class II bacteriocins are a class of small peptides that inhibit the growth of various bacteria. Many Gram-positive bacteria ...
Bacteriocin
... large plasmids, small plasmids, chromosomal), molecular weight and chemistry (large protein, peptide, with/without sugar moiety ... Colicins are bacteriocins found in the Gram-negative E. coli. Similar bacteriocins (CLBs, colicin-like bacteriocins) occur in ... Wikimedia Commons has media related to Bacteriocin. Bagel Bacteriocin Database BACTIBASE Database Bacteriocins at the US ... They are the longest studied bacteriocins. They are a diverse group of bacteriocins and do not include all the bacteriocins ...
Lacticaseibacillus rhamnosus
This sample did not contain any plasmids. The most extensively studied strain, L. rhamnosus GG, a gut isolate, consists of a ... bacteriocin production, pili production, the CRISPR-Cas system, the clustered regularly interspaced short palindromic repeat ( ... CRISPR) loci, and more than 100 transporter functions and mobile genetic elements such as phages, plasmid genes, and ...
Streptomyces ipomoeae
... and Plasmid Profiles". Phytopathology. 88 (11): 1179-86. doi:10.1094/phyto.1998.88.11.1179. PMID 18944851. Bouarab, edited by ... Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene". Applied and ... Purification and Characterization of a Highly Specific Bacteriocin and Cloning of Its Structural Gene". Applied and ...
Agrobacterium tumefaciens
The two plasmids are pTiC58, responsible for the processes involved in virulence, and pAtC58, once dubbed the "cryptic" plasmid ... K84 produces a bacteriocin (agrocin 84) which is an antibiotic specific against related bacteria, including A. tumefaciens. ... To be virulent, the bacterium contains tumour-inducing plasmid (Ti plasmid or pTi), of 200 kbp, which contains the T-DNA and ... PMID 32915125.{{cite journal}}: CS1 maint: uses authors parameter (link) "At plasmid" when talking about related plasmids Smith ...
Lactobacillus
Many lactobacilli also contain multiple plasmids. A recent study has revealed that plasmids encode the genes which are required ... The antibacterial and antifungal activity of lactobacilli relies on production of bacteriocins and low molecular weight ... Davray D, Deo D, Kulkarni R (November 2020). "Plasmids encode niche-specific traits in Lactobacillaceae". Microbial Genomics. 7 ... Lactobacilli produce bacteriocins to suppress pathogenic growth of certain bacteria, as well as lactic acid and H2O2 (hydrogen ...
Gluconacetobacter diazotrophicus
This genome has one circular chromosome and two plasmids. The two plasmids found in the genome are pGD01 and pGD02 which ... produces a bacteriocin against Xanthomonas albilineans, a sugar cane pathogen". Research in Microbiology. 153 (6): 345-351. doi ...
Bacillus cereus
Plasmid pBCE4810 shares homology with the B. anthracis virulence plasmid pXO1, which encodes the anthrax toxin. Periodontal ... Naclerio G, Ricca E, Sacco M, De Felice M (December 1993). "Antimicrobial activity of a newly identified bacteriocin of ... structure and location on a mega virulence plasmid related to Bacillus anthracis toxin plasmid pXO1". BMC Microbiology. 6: 20. ... It was shown independently by two research groups to be encoded on multiple plasmids: pCERE01 or pBCE4810. ...
Antibiotic use in livestock
These conjugative plasmids carry a number of genes that can be assembled and rearranged, which could then enable bacteria to ... Another research team was able to use bacteriocins, antimicrobial peptides and bacteriophages in the control of bacterial ... Bennett P. M. (2008). Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in ... Joerger R.D. (2003). "Alternatives to antibiotics: bacteriocins, antimicrobial peptides and bacteriophages". Poultry Science. ...
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Genes for bacteriocins may reside on plasmids.. Read more about this term in the Online Textbook:. Emerging Infectious Diseases ... Bacteriocin. Proteins produced by some bacteria, which inhibit the growth of other strains of the same organism or related ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ...
Bivariate Data and Analysis Glossary - Annenberg Learner
Genes for bacteriocins may reside on plasmids.. Read more about this term in the Online Textbook:. Emerging Infectious Diseases ... Bacteriocin. Proteins produced by some bacteria, which inhibit the growth of other strains of the same organism or related ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ... A plasmid vector used to clone large fragments of DNA (average size of 150 kb) in E. coli. ...
Frontiers | Colicins and Salmocins - New Classes of Plant-Made Non-antibiotic Food Antibacterials
Potential Markets for Bacteriocins as Food Antibacterials. Bacteriocins such as colicins and salmocins are promising ... Plasmid Constructs. Constructs used were described in Schulz et al. (2015) or Schneider et al. (2018). ... Table 1. Bacteriocins versus antibiotics: major biological differences and market potential of bacteriocins. ... 2018). Plant-made Salmonella bacteriocins salmocins for control of Salmonella pathovars. Sci. Rep. 8:4078. doi: 10.1038/s41598- ...
Pediococcus pentosaceus Mees - 43200 | ATCC
Academic Staff
Microbial production of bacteriocins and its applications in food preservation: A review.
Bacteriocins, a heterogeneous group of bioactive bacterial peptides or proteins, are ribosomally synthesized that inhibit or ... Genes encoding these bacteriocins can be present either on the plasmid or chromosome as a subset of three genes, bacteriocin ... Classification of Bacteriocins. Bacteriocins are classified into different groups (Table 2). Class I bacteriocins (lantibiotics ... Table 6: Potential applications of bacteriocin in various formulations sector.. Bacteriocin Toxicity. Bacteriocins have been ...
Pediocin PA-1 and a pediocin producing Lactobacillus plantarum strain do not change the HMA rat microbiota<...
The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut ... Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L ... N2 - The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal ... AB - The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal ...
Plant genotype, micronutrient fertilization and take-all infection influence bacterial populations in the rhizosphere of wheat ...
Plasmid-determined Bacteriocin Production by Rhizobium leguminosarum. A - Papers appearing in refereed journals ... Conjugative plasmids in Rhizobium. Abstracts ESF Workshop on Plasmid Mediated Gene Transfer, University of Birmingham, 12-15 ... Ecology of plasmid transfer and spread. in: Thomas, C. M. (ed.) The horizontal gene pool: bacterial plasmids and gene spread ... Abstracts 3rd ESF Workshop on Molecular Biology and Ecology of Plasmid-Mediated Gene Transfer, Cuenca, 12-16 September 1997 . ...
IMSEAR at SEARO: Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae.
Bacteriocin Plasmids. en_US. dc.subject.mesh. Colicins --biosynthesis. en_US. dc.subject.mesh. Drug Resistance, Microbial. en_ ... Sharma PL, Sharma KB, Prakash K. Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. Indian Journal ... Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae.. en_US. ...
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Genes of the sbo-alb locus of Bacillus subtilis are required for production of the antilisterial bacteriocin subtilosin<...
A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ... A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. The plasmid insertion ...
Archive | Journal of Microbiology, Biotechnology and Food Sciences
DeCS 2018 - July 31, 2018 version
Genes11
- Three LAB strains (firstly named as Lactobacillus curvatus 12, L. curvatus 36 and Weissella viridescens 23) were obtained from calabresa by presenting promising bacteriocinogenic activity, distinct genetic profiles (rep-PCR, RAPD, bacteriocin-related genes) and wide inhibitory spectrum. (biomedcentral.com)
- Plasmids are, however, much smaller than the bacterial chromosome, usually only containing a few genes. (scienceprofonline.com)
- Plasmids called R factors carry genes for antibiotic resistance, or resistance to chemicals that, without the plasmid, would be toxic to the bacterium. (scienceprofonline.com)
- Bacteriocin factors carry genes for making proteins that kill other bacteria that do not have this factor, reducing competition for the bacteria that have it. (scienceprofonline.com)
- Other plasmids, called virulence factors have genes that help bacteria cause disease, such as the productions exotoxins that make those infected with the bacterium ill, or genes for adhesins, that allow the bacterium to adhere to and infect certain host cell types. (scienceprofonline.com)
- The predicted protein-coding genes, which are encoding the CRISPR-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. (ejast.org)
- These include a large number of genes that code for secreted toxins and enzymes, as well as genes that encode products for the production of antibiotics and bacteriocins. (up.ac.za)
- The observed distribution of resistance plasmids and β-lactamase genes in several clones indicates a high degree of horizontal transfer. (cdc.gov)
- Analysis of the 5.04 MB chromosomal genome predicts 4599 protein coding genes, seven sets of ribosomal RNA genes, 84 tRNA genes and a 64.8 KB plasmid encoding 74 genes. (uncg.edu)
- SCBI (South African Caenorhabditis briggsae Isolate) are found in ~29 genomic islands of 5 to 65 genes and are enriched in putative functions that are biologically relevant to an entomopathogenic lifestyle, including non-ribosomal peptide synthetases, bacteriocins, fimbrial biogenesis, ushering proteins, toxins, secondary metabolite secretion and multiple drug resistance/efflux systems. (uncg.edu)
- Multiple plasmid-borne virulence genes of Clavibacter michiganensis ssp. (uni-bielefeld.de)
Located on plasmid pPCP12
- Sequencing and analysis of the ( pla ) gene, located on plasmid pPCP1, the answers. (cdc.gov)
- The plasminogen activator/coagulase ( pla ) gene, located on plasmid pPCP1, is incorporated into most Y. pestis PCR assays, and in several studies it was the prime or sole marker ( 1 , 2 , 5 , 7 - 9 ). (cdc.gov)
Microbial2
- Microbial production of bacteriocins and its applications in food preservation: A review. (alliedacademies.org)
- Garai B, Purkait A, Ghose T ,et al.Microbial production of bacteriocins and its applications in food preservation: A review. (alliedacademies.org)
Determinant1
- Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6. (hammamilab.org)
Yersinia1
- The type III system is closely related to the Yersinia plasmid-encoded type III system. (up.ac.za)
Putative1
- We hypothesize the putative bacteriocin, transposase, and N-acetylmuramoyl-L-alanine amidase, based on their DNA sequences, are involved in lysis. (emerging-researchers.org)
Closely related3
- Bacteriocins are a large family of ribosomally synthesized proteinaceous toxins produced by bacteria and Archaea that have antimicrobial activity against bacteria closely related to the producer strain [ 9 ]. (alliedacademies.org)
- Bacteriocins are protein compounds that present a variable spectrum of antimicrobial activity, usually against closely related species to the producer strains [ 3 ]. (biomedcentral.com)
- Although plasmids are most often exchanged between closely related bacteria, some plasmids can be shared by very dissimilar microbes. (scienceprofonline.com)
Lactic2
- Bacteriocins produced by lactic acid bacteria (LAB) can be considered as viable alternatives for food safety and quality, once these peptides present antimicrobial activity against foodborne pathogens and spoilage bacteria. (biomedcentral.com)
- However, only six isolates among which E. faecalis 14, E. faecalis 28, E. faecalis 90, E. faecalis 97, and E. faecalis 101 (obtained from donor 3), and E. faecalis 93 (obtained from donor 5) were active against some Gram-negative bacteria and Gram-positive bacteria, through production of lactic acid, and bacteriocin like inhibitory substances. (cyberleninka.org)
Inhibitory2
- Partially purified bacteriocins from L. curvatus 12 kept their inhibitory activity after elution with isopropanol at 60% ( v /v). Bacteriocins produced by this strain were purified by HPLC and sequenced, resulting in four peptides with 3102.79, 2631.40, 1967.06 and 2588.31 Da, without homology to known bacteriocins. (biomedcentral.com)
- Among these isolates, bacteriocins produced by L. curvatus 12, now named as L. curvatus UFV-NPAC1, presented the highest inhibitory performance and the purification procedures revealed four peptides with sequences not described for bacteriocins to date. (biomedcentral.com)
Genetic5
- Genetic determinants of lactose fermentation, proteolytic activity and bacteriocin production in the strains L. lactis subsp. (sdu.edu.tr)
- As a result of mutation and genetic transfer studies, it was determined that ail of these characters were encoded by the 65.4 kb plasmid in L. lactis subsp. (sdu.edu.tr)
- In order to transfer a plasmid, the bacterium with the plasmid must have the genetic instructions required to construct a sex pilus-a prokaryotic cell extension that can hook up to a recipient bacterium, connecting the two bacteria and physically pulling them towards each other. (scienceprofonline.com)
- Genetic structure of the Enterococcus faecalis plasmid pAD1-encoded cytolytic toxin system and its relationship to lantibiotic determinants. (researchsnappy.com)
- a Genetic locus producing the antimicrobial activity and alignment with homologous loci found on plasmid pBac115 of an S. epidermidis strain and on the chromosome of B. cereus ATCC14579, described to produce the thiopeptide micrococcin P1. (biomedcentral.com)
Proteins1
- Bacteriocins, a heterogeneous group of bioactive bacterial peptides or proteins, are ribosomally synthesized that inhibit or kill other related or unrelated microorganisms. (alliedacademies.org)
Gene5
- A mutant defective in production of the Substance was isolated from a plasmid gene disruption library. (elsevier.com)
- The plasmid insertion conferring the antilisterial-peptide- negative phenotype was located in a seven-gene operon (alb, for antilisterial bacteriocin) residing immediately downstream from the sbo gene, which encodes the precursor of subtilosin. (elsevier.com)
- To investigate whether the presence of the pla gene sequences indicated the presence of the carrier pPCP1 plasmid in Y. pestis , we designed PCR assays for the amplification of 3 conserved regions of this plasmid ( Technical Appendix ). (cdc.gov)
- Inducible rescue plasmids containing functional gene inserts will be transformed into knockout P. larvae cultures to restore the wildtype phenotype. (emerging-researchers.org)
- A TaqMan real-time PCR for identification of B. anthracis was developed, based on the two plasmids, pX01 and pX02, both of which are necessary for pathogenicity, as well as on the chromosomally encoded rpoB gene, to confirm or exclude potential attacks approximately 2-3 h after the material has arrived in the laboratory. (semanticscholar.org)
Assays1
- Multiple PCR assays for Y. pestis detection that primarily detect markers located on plasmids have been developed ( 1 - 6 ). (cdc.gov)
Escherichia1
- In 2011, Germany experienced the largest outbreak with a Shiga toxin-producing Escherichia coli (STEC) strain ever recorded, and researchers explored bacteriophages, bacteriocins, and low-molecular-weight inhibitors against STEC. (semanticscholar.org)
Strains4
- Effects of ingested (i) pediocin PA-1 producing Lactobacillus plantarum DDEN 11007, (ii) the plasmid cured pediocin negative L. plantarum DDEN 12305, or (iii) supernatants of either of these two strains on the composition of the intestinal microbiota of Human Microbiota Associated (HMA) rats were examined by selective cultivation and molecular methods. (dtu.dk)
- Fermented foods, such as artisanal sausages and cured meats, are relevant sources of LAB strains capable of producing novel bacteriocins, with particular interest by the food industry. (biomedcentral.com)
- Among these strains, L. curvatus 12 presented higher bacteriocin production, reaching 25,000 AU/mL after incubation at 25, 30 and 37 °C and 6, 9 and 12 h. (biomedcentral.com)
- Thus, the present study aimed to isolate bacteriocinogenic LAB strains from artisanal meats produced in Minas Gerais state, Brazil, and to characterize their produced bacteriocins towards their antimicrobial features and structure. (biomedcentral.com)
Bacteria2
- Can they increase the colonizing capability of plasmid carrying bacteria? (antimicrobialresistance.eu)
- Numerous bacteriocins produced by LAB species have been already described and they are well known by their activity against spoilage bacteria and foodborne pathogens [ 3 ]. (biomedcentral.com)
Strain2
- diacetylactis MPD166 strain were found to be responsible for the 61.0 kb, 14.2 and 9.0 kb plasmids respectively. (sdu.edu.tr)
- The complete genome sequences of the strain contained one circular chromosome (2,057,809 bp) with 38.2% GC content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. (ejast.org)
Production3
- IMSEAR at SEARO: Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. (who.int)
- Sharma PL, Sharma KB, Prakash K. Colicin production & coexistence of Col+ plasmid with R-plasmid in Salmonellae. (who.int)
- Lactose fermentation and proteolytic activity were encoded by the 68.0 kb plasmid and bacteriocin production was encoded by the 12.4 kb plasmid in L. lactis subsp. (sdu.edu.tr)
Molecules1
- By electron microscopy, restriction analysis and DNA sequencing, it was demonstrated that the phage and the plasmid DNAs are linear, circularly permuted molecules. (semanticscholar.org)
Infection2
- A plasmid is a molecule of DNA, independent of the bacterial nucleoid chromosome, which often has 'bonus DNA' with instructions for weapons of infection. (scienceprofonline.com)
- During this period Photorhabdus luminescens releases bacteriocidal products, including antibiotics and bacteriocins, that prevent infection of the larva by competitive microbes. (up.ac.za)
Microbiota2
- The bacteriocin pediocin PA-1 has potential use as a food biopreservative, and understanding its effect on the commensal gut microbiota is important for assessment of consumer risks associated with the use of biopreservative cultures. (dtu.dk)
- Pediocin PA-1 did not mediate changes of the rat microbiota, and a biological assay indicated that the bacteriocin was degraded or inactivated during passage through the intestine. (dtu.dk)
Sequences1
- Comparison of the presubtilosin and mature subtilosin sequences suggested that certain residues undergo unusual posttranslational modifications unlike those occurring during the synthesis of class I (lantibiotic) or some class II bacteriocins. (elsevier.com)
Species2
- A donor and a recipient do not always need to be of the same species in order to share a plasmid. (scienceprofonline.com)
- A plasmid that can replicate in one, or at most a few, different bacterial species. (expertglossary.com)
Bacterial1
- Unlike the bacterial chromosome, plasmid traits are not normally required for the cell to function, but do help a bacterium survive and cause disease. (scienceprofonline.com)
Factors1
- There are many different types of plasmids, in addition to F factors mentioned above. (scienceprofonline.com)
Analysis2
- Amino acid analysis confirmed that the substance was the cyclic bacteriocin subtilosin. (elsevier.com)
- Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis. (hammamilab.org)
Food1
- Since they pose no health risk concerns, soon bacteriocins are largely dependent on their use as safe food preservatives. (alliedacademies.org)
Potential1
- Besides, this article stated the potential applications of bacteriocin in the bactericidal formulations sector. (alliedacademies.org)