A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Amino Acid Sequence
Gram-Positive Asporogenous Rods
A genus of gram-positive, rod-shaped bacteria whose cells occur singly, in pairs or short chains, in V or Y configurations, or in clumps resembling letters of the Chinese alphabet. Its organisms are found in cheese and dairy products as well as on human skin and can occasionally cause soft tissue infections.
Sequence Analysis, DNA
Drug Resistance, Microbial
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Gene Expression Regulation, Bacterial
Open Reading Frames
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Microbial Sensitivity Tests
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Isolation and characterization of ColE1-derived plasmid copy-number mutant. (1/196)The plasmid pBGP120 is a ColE1 derivative that contains elements of the Escherichia coli lac operon and the Tn3 transposon. We have selected and isolated a copy-number mutant of pBGP120. In exponentially growing cultures, the copy-number mutant, pOP1, represents approximately 30% of total intracellular DNA compared to about 5% for pBGP120. Plasmid-encoded beta-galactosidase monomer can represent 50% of newly synthesized protein in cells carrying pOP1. pOP1 is structurally unstable in certain genetic backgrounds and under certain growth conditions, breaking down to a smaller sized plasmid that retains the DNA overproducer phenotype and the Tn3 transposon. The smaller overproducer plasmid, pOP1delta6, is generated by a continuous deletion of sequences located between one end of the Tn3 transposon and a site about 630 nucleotides from the EcoRI site in the beta-galactosidase structural gene of pOP1. pOP1delta6 retains the ColE1 origin of replication but has lost the lac promotor and operator and most of the beta-galactosidase structural gene. pOP1delta6 exists at approximately 210 copies per chromosome in exponentially growing cells. (+info)
Analysis of the CoIE1 stability determinant Rcd. (2/196)Multimer formation is an important cause of instability for many multicopy plasmids. Plasmid CoIE1 is maintained stably because multimers are converted to monomers by Xer-mediated site-specific recombination at the cer site. However, multimer resolution is not the whole story; inactivation of a promoter (Pcer) within cer causes plasmid instability even though recombination is unaffected. The promoter directs the synthesis of a short transcript (Rcd) which is proposed to delay the division of multimer-containing cells. Mapping of the 5' terminus of Rcd confirms that transcription initiates from Pcer. The 3' terminus shows considerable heterogeneity, consistent with a primary transcript of 95 nt being degraded via intermediates of 79 and 70 nt. Secondary structure predictions for Rcd are presented. Of four mutations which abolish Rcd-mediated growth inhibition, one reduces the activity of Pcer while the other three map to the rcd coding sequence and reduce the steady-state level of the transcript. RNA folding analysis suggests that these three mutant transcripts adopt a common secondary structure in which the major stem-loop differs from that of wild-type Rcd. A survey of 24 cer-like multimer resolution sites revealed six which contain Pcer-like sequences. The putative transcripts from these sites have similar predicted secondary structures to Rcd and contain a highly conserved 15 base sequence. To test the hypothesis that Rcd acts as an anti-sense RNA, interacting with its target gene(s) through the 15 nt sequence, we used DNA hybridization and sequence analysis to find matches to this sequence in the Escherichia coli chromosome. Our failure to find plausible anti-sense targets has led to the suggestion that Rcd may interact directly with a protein target. (+info)
Expression of leading region genes on IncI1 plasmid ColIb-P9: genetic evidence for single-stranded DNA transcription. (3/196)The leading region of a plasmid is the first sector to enter the recipient cell in bacterial conjugation. This sector of IncI1 plasmid ColIb-P9 includes genes that are transcribed in a transient pulse early in the conjugatively infected cell to promote establishment of the immigrant plasmid. Evidence is presented that the burst of gene expression is regulated by a process which is independent of a repressor but dependent on the orientation of the genes on the unique plasmid strand transferred in conjugation. The nucleotide sequence of 11.7 kb of the leading region was determined and found to contain 10 ORFs; all are orientated such that the template strand for transcription corresponds to the transferred strand. The leading region contains three dispersed repeats of a sequence homologous to a novel promoter in ssDNA described by H. Masai & K. Arai (1997, Cell 89, 897-907). It is proposed that the repeats are promoters that form in the transferring strand of ColIb to support transient transcription of genes transferred early in conjugation. (+info)
Features of distamycin preferential binding sites on natural DNA predicted using differential scanning calorimetry. (4/196)The interaction of distamycin with ColE1 DNA was examined by using differential scanning calorimetry (DSC) taking the helix-coil transition theory of DNA into consideration. Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends. (+info)
Monomer-dimer control of the ColE1 P(cer) promoter. (5/196)XerCD-mediated recombination at cer converts multimers of plasmid ColE1 to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. In addition to XerCD, recombination requires the accessory factors ArgR and PepA. The promoter P(cer), located centrally within cer, is also required for stable plasmid maintenance. P(cer) is active in plasmid multimers and directs transcription of a short RNA, Rcd, which appears to inhibit cell division. It has been proposed that Rcd is part of a checkpoint which ensures that multimer resolution is complete before the cell divides. This study has shown that ArgR does not act as a transcriptional repressor of P(cer) in plasmid monomers. P(cer) is unusual in that the -35 and -10 hexamers are separated by only 15 bp and this study has demonstrated that increasing this to a more conventional spacing results in elevated activity. An increase to 17 bp resulted in a 10- to 20-fold increase in activity, while smaller effects were seen when the spacer was increased to 16 bp or 18 bp. These observations are consistent with the hypothesis that P(cer) activation involves realignment of the -35 and -10 sequences within a recombinational synaptic complex. This predicts that a 17 bp spacer promoter derivative should be down-regulated by plasmid multimerization, and this is confirmed experimentally. (+info)
Purification and characterization of a proteolytic active fragment of DNA topoisomerase I from the brine shrimp Artemia franciscana (Crustacea Anostraca). (6/196)The ATP-independent type I topoisomerase from the crustacean Artemia franciscana was purified to near-homogeneity. Its activity was measured by an assay that uses the formation of an enzyme-cleaved DNA complex in the presence of the specific inhibitor camptothecin. The purification procedure is reported. Purified topoisomerase is a single-subunit enzyme with a molecular mass of 63 kDa. Immunoblot performed on the different steps of purification shows that the purified 63 kDa peptide is a proteolytic fragment of a protein with a molecular mass of 110 kDa. Similarly to the other purified eukaryotic topoisomerases, the crustacean enzyme does not require a bivalent cation for activity, but is stimulated in the presence of 10 mM-MgCl2; moreover, it can relax both negative and positive superhelical turns. The enzyme activity is strongly inhibited by the antitumour drug camptothecin. The enzyme inhibition is related to the stabilization of the cleavable complex between topoisomerase I and DNA. (+info)
Structure of the ColE1 DNA molecule before segregation to daughter molecules. (7/196)The segregation of daughter DNA molecules at the end stage of replication of plasmid ColE1 was examined. When circular ColE1 DNA replicates in a cell extract at a high KCl concentration (140 mM), a unique class of molecules accumulates. When the molecule is cleaved by a restriction enzyme that cuts the ColE1 DNA at a single site, an X-shaped molecule in which two linear components are held together around the origin of DNA replication is made. For a large fraction of these molecules, the 5' end of the leading strand remains at the origin and the 3' end of the strand is about 30 nucleotides upstream of the origin. The 3' end of the lagging strand is located at the terH site (17 nucleotides upstream of the origin) and the 5' end of the strand is a few hundred nucleotides upstream of the terH site. Thus the parental strands of the molecule intertwine with each other only once. When the KCl concentration is lowered to 70 mM, practically all of these molecules are converted to daughter circular monomers or to catenanes consisting of two singly interlocked circular units. (+info)
A new colicin that adsorbs to outer-membrane protein Tsx but is dependent on the tonB instead of the tolQ membrane transport system. (8/196)A new colicin, Col5, was synthesized by an Escherichia coli isolate of human origin from the ECOR Collection. It was unique because it adsorbed to the outer-membrane protein Tsx, but used the tonB rather than the tolQ membrane transport system, which is employed by the only other Tsx-specific colicin, ColK. Col5 was encoded by a 5.2 kb plasmid, p5. It was inducible by mitomycin C, and strains harbouring p5 exhibited quasi-lysis. The bactericidal protein had an M(r) of 56,000. (+info)
Microbiology Society Journals | Evolution of colicin BM plasmids: the loss of the colicin B activity gene
Colicins, a class of antimicrobial compounds produced by bacteria, are thought to be important mediators of intra- and interspecific interactions, and are a significant factor in maintaining microbial diversity. Colicins B and M are among the most common colicins produced by Escherichia coli, and are usually encoded adjacently on the same plasmid. In this study, the characterization of a collection of E. coli isolated from Australian vertebrates revealed that a significant fraction of colicin BM strains lack an intact colicin B activity gene. The colicin B and M gene region was sequenced in 60 strains and it was found (with one exception) that all plasmids lacking an intact colicin B activity gene have an identical colicin gene structure, possessing a complete colicin B immunity gene and a 130 bp remnant of the B activity gene. A phylogenetic analysis of the colicin M and B operons and characterization of the plasmids suggested that ColBM plasmids with a truncated B activity gene have evolved on at
ColV Plasmid-Mediated, Colicin V-Independent Iron Uptake System of Invasive Strains of Escherichia coli | Infection and Immunity
Evidence is presented that ColV plasmid-mediated iron uptake, an important component of the virulence of invasive strains of Escherichia coli, is independent of colicin V synthesis and activity. A mutant of E. coli K-12 deficient in the biosynthesis of enterochelin (strain AN1937) was unable to grow on minimal agar containing the chelating agent α,α′-dipyridyl unless it was harboring the plasmid ColV-K30 (strain LG1315). Acquisition of the active plasmid-specified iron sequestering system was accompanied by marked enhancement of pathogenicity in experimental infections of mice. Mutants of strain LG1315 were isolated that were defective in iron uptake due to plasmid mutations. They were unchanged with respect to colicin production, but were significantly less virulent than the parent strain. Conversely, mutants isolated as defective in colicin V synthesis were normal for the plasmid-coded iron uptake mechanism and showed the same lethality for infected mice as did strain LG1315. Furthermore, ...
P-type potassium transmembrane transporter activity Gene Ontology Term (GO:0008556)
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
serine-type endopeptidase activity Gene Ontology Term (GO:0004252)
The Gene Ontology (GO) project is a collaborative effort to address the need for consistent descriptions of gene products across databases. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated gene data at MGI are provided in Term Detail reports.
High Levels of Colicin Resistance in Escherichia coli | Riley Lab
Colicins are plasmid-encoded antibiotics that are produced by and kill Escherichia coli and other related species. The frequency of colicinogeny is high, on average 30% of E. coli isolates produce colicins. Initial observations from one collection of 72 strains of E. coli (the ECOR collection) suggest that resistance to colicin killing is also ubiquitous, with over 70% of strains resistant to one or more colicins. To determine whether resistance is a common trait in E. coli, three additional strain collections were surveyed. In each of these collections levels of colicin production were high (from 15 to 50% of the strains produce colicins). Levels of colicin resistance were even higher, with most strains resistant to over 10 colicins. A survey of 137 non-E. coli isolates revealed even higher levels of resistance. We discuss a mechanism (pleiotropy) that could result in the co-occurrence of such high levels of colicin production and colicin resistance ...
Majd 4 kil s m rna a horgon | H dmez v s rhely h rek | Szeged - delmagyar.hu
Mindszent - A mindszenti horg sz, G l Istv n a k zelm ltban ritkas gnak sz m t 3,86 kil s m rn t fogott a Tisz n a Kurca-torkolatn l, m lt szombaton pedig egy 9 kil s amurt. Sikereinek titka speci lis etet anyaga.
Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky...
Horizontal Gene Transfer of a ColV Plasmid Has Resulted in a Dominant Avian Clonal Type of Salmonella enterica Serovar Kentucky. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Controlled secretion into the culture medium of a hybrid beta-glucanase by Acetobacter methanolicus mediated by the kil gene of...
A Tn5-based transposon bearing the kil gene (killing protein), mediating controlled export of periplasmic proteins into the culture medium, was constructed (Tn5-KIL3). This transposon contained the kil gene of the ColE1 plasmid under the growth-phase-dependent promoter of the fic gene (filamentation induced by cAMP) of Escherichia coli, an interposon located upstream of kil, a kanamycin/neomycin-resistance gene, a multiple cloning site and the mob site. The transposition of Tn5-KIL3 to Acetobacter methanolicus showed a moderate transposition frequency (10(-5)-10(-6)). By insertion of a Bacillus hybrid beta-glucanase (bgl) as a model protein into the transposon (Tn5-LF3) it was shown that the secretion function as well as the gene of the target protein had been transferred to and stably integrated into the chromosome of A, methanolicus, and that the transposition of Tn5-LF3 was non-specific. beta-Glucanase was highly overexpressed and secreted into the medium during stationary phase. Total and ...
i-K+ - intermediate-conductance K+ channel | AcronymAttic
How is intermediate-conductance K+ channel abbreviated? i-K+ stands for intermediate-conductance K+ channel. i-K+ is defined as intermediate-conductance K+ channel rarely.
Blockade of U50488H on potassium currents of acutely isolated mouse hippocampal CA3 pyramidal neurons - CORE
The actions of the opioid agonist U50488H on I-A and I-K were examined in acutely isolated mouse hippocampal CA3 pyramidal neurons using the whole-cell patch clamp technique. U50488H caused a concentration dependent, rapidly developing and reversible inhibition of voltage-activated I-A and I-K. The inhibitory actions were still observed in the presence of 30 muM naloxone or 5 muM nor-binaltorphimine dihydrochloride. The IC50 values for the blockade of I-A and I-K were calculated as 20.1.9 and 3.7 muM, respectively. In the presence of 3.3 muM U50488H, repetitive stimulation induced use-dependent inhibition of I-A and I-K. A 10 muM concentration of U50488H positively shifted the half-activation membrane potential of I-A by +11 mV, but negatively shifted I-K by -14 mV. These results demonstrate that U50488H can directly inhibit neuronal I-A and I-K without involvement of the activation of kappa -opioid receptors. (C) 2001 Elsevier Science B.V. All rights ...
Samsung CAD300MBEC nab je ka do auta pro C170, D520 a dal ern (EU blister) - Huramobil.cz
Samsung CAD300MBEC nab je ka do auta pro C170, D520 a dal ern (EU blister) od v robce SAMSUNG m me skladem jen za 99 K . Osobn odb r nebo dod n do 24 hodin kdekoli v R. Nakupte od specialist na mobiln telefony.
Extended definition essay laziness meme
He will feel discontent and extended with himself, complain more than usual, and he essay have a laziness, emotional, physical, and spiritual collapse in a way Colicin v synthesis protein shakes. A lazy person tends to delay things or have someone else do the work, thinking that he cannot do it on his own and that his essay is inferior among the others knowledge, and be extended unfit.
Imm, meaning words Imm, what is Imm, word mean something Imm - Wikiwordbook.info
Our service is a large database of words. You can use our database for the selection of a name for their company website, and the like. Also, you learn the value of the most unusual words
Muratore:Materials/Plasmids/pTXB1His - OpenWetWare
1 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATAAC TACGTCAGGT 51 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 101 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 151 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 201 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 251 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 301 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 351 CGCCCCGAAG AACGTTCTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 401 TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 451 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 501 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 551 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 601 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 651 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 701 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 751 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 801 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 851 ...
Imm trynna make me some GAINS! - AnabolicMinds.com
The time has finally come, ive been planning this cycle for a while now and its about go down. Cycle: (weeks 1-8) Test E: 500/250/0/0/... Msten: 14/14/
Pantothenyl alcohol | Article about pantothenyl alcohol by The Free Dictionary
Looking for pantothenyl alcohol? Find out information about pantothenyl alcohol. any of a class of organic compounds with the general formula R-OH, where R represents an alkyl group made up of carbon and hydrogen in various proportions... Explanation of pantothenyl alcohol
Leicester Research Archive: Plasmid-mediated restriction evasion mechanisms
Plasmid ColIb-P9 (Incll) encodes mechanisms which allow it to avoid destruction by type I and type II restriction enzymes during transfer by conjugation between strains of Escherichia coli. A genetic system was developed to analyse these mechanisms. The system relied on measuring Collb-mediated rescue of the restriction-sensitive plasmid R751 (IncPp) from destruction by EcoKI (type I) and EcoRI (type II). One Collb mechanism was known to involve a plasmid-encoded antirestriction gene known as ardA, the product of which is active against type I enzymes. Tests for alleviation of EcoKI restriction of R751, showed strong protection by a co-transferring Collb (Ard+) plasmid, slight protection when Collb was resident in the recipient and no effect when Collb was immobilised in the donor by removal of its nic site. Hence, expression of ardA is activated in the recipient cell following transfer no detectable transfer of the ArdA protein occurs from the donor to the recipient. The ardA gene is found in ...
Kilómetro 658 (Río Primero, Córdoba, Argentina) - Population Statistics, Charts, Map, Location, Weather and Web Information
Kilómetro 658 (Río Primero, Córdoba, Argentina) with population statistics, charts, map, location, weather and web information.
Investigating the molecular mechanisms of colicin import into Escherichia coli cells - Nottingham ePrints
This work has studied colicin translocation by using a periplasmic protection assay combined with an in vivo lux-reporter assay and a potassium release assay. Expressing the translocation domain of colicin A in the periplasm and challenging the cells with external colicins showed that the translocation of group A colicins is inhibited as it requires an interaction with the Tol system. Surprisingly, the TolA protein was found to play the major role during the translocation of both ColA and the endonuclease colicin E9 even though the latter colicin has no direct interaction with the TolA protein. This study also suggests that the interaction with TolB is important for both colicins A and E9. Moreover, a series of ColA constructs with a truncated T domain were made by site directed mutagenesis to define the important residues of the TolA and TolB boxes for their interaction with Tol proteins. The results showed that tyrosine 58 residue of the TolA box of colicin A is essential for TolA binding, ...
Characterization of a Theta-Type Plasmid from Lactobacillus sakei: a Potential Basis for Low-Copy-Number Vectors in...
pRV500 is, to our knowledge, the first plasmid of L. sakei to be entirely sequenced. Careful sequence analysis showed that pRV500 belongs to the pUCL287 family, itself related to class A theta-type plasmids of the pUCL22 and pSC101 families. Experimental evidence of the theta replication mode was obtained for pUCL287 itself (2). Although the minimum length of the pRV500 oriA region remains to be determined, it is likely to involve the same two kinds of DNA repeats as other pUCL287-type plasmids. The involvement of repetitive sequences of 22 bp (iterons) as a target for binding of the Rep protein was demonstrated for pSBO1 (40), and the upstream 11-bp repeated sequences were observed to be essential for pUCL287 replication (3).. Like other plasmids (14, 49), pRV500 appears to be a composite structure containing DNA segments from different sources. Some ORFs are shared by plasmids from both subfamilies pUCL22 and pUCL287; this applies to orf6 in pRV500 and ORFs of unknown function around the gene ...
Membrane action of colicin E1: detection by the release of carboxyfluorescein and calcein from liposomes. | Semantic Scholar
Colicin E1 induces the efflux of carboxyfluorescein and calcein from liposomes whose phospholipid composition is similar to that of Escherichia coli. This colicin action takes place at protein-to-liposome ratios and within pH ranges that are physiologically meaningful. Colicin-induced permeability of carboxyfluorescein is not limited to the initial phase of colicin membrane interaction but is sustained thereafter. Colicin E1 requires negatively charged phospholipids in the liposomal membrane in order to bind and induce efflux.
MovingPennies | Penny Stocks Stock News Cheap Stocks
COLV is on heavy alert this morning! 6/1/2015. COLV has a last price of .10c a share. COLV is a company with plans to completely REVOLUTIONIZE the way we drink coffee, tea or other hot beverages. This past Fall, the company acquired Simply Lids Inc. - an award winning company specializing in disposable beverage lids that prevent spills and offer a better drinking experience!. Simply Lids patented technologies provide a safer, more enjoyable drinking experience, without splashing or spills, and have the added benefit of unique marketing opportunities, never before realized in this industry sector. Simply Lids plans on being the leader in this multi-billion dollar sector through the application of its unique technology and innovation.. Current disposable lids on the market require you to sip your drink out of a tiny hole. They leak, splash, and can cause hot liquid to burn your mouth. Nothing about that is natural, and it takes away from the full enjoyment.. The patented Simply Slider™ provides ...
Soal Test TOEFL saat TOEFL Prediction for MBEC 2008 ~ Tips dan Trik Belajar TOEFL | TOEIC | iBT Online
Tips dan Trik Belajar TOEFL | TOEIC | iBT Online: Soal Test TOEFL saat TOEFL Prediction for MBEC 2008 - Belajar TOEFL, TOEIC, IELTS dan Bahasa Inggris online di sini. Mudah, cepat, tepat, download free e-book dan tutorial bahasa Inggris gratis.
Linear diagram showing the approximate lengths of whole | Open-i
Linear diagram showing the approximate lengths of whole colicin Ia and the four carboxy-terminal fragments, CT-S, CT-M, CT-L, and CT-XL. The boundaries of the T
Institute for Genome Sciences - Publications Archive
Theunissen TW, van Oosten AL, Castelo-Branco G, Hall J, Smith A, Silva JC. Nanog overcomes reprogramming barriers and induces pluripotency in minimal conditions, Current biology : CB. 2010-12-30 Glass E, Meyer F, Gilbert JA, Field D, Hunter S, Kottmann R, Kyrpides N, Sansone S, Schriml L, Sterk P, White O, Wooley J. Meeting Report from the Genomic Standards Consortium (GSC) Workshop 10, Standards in genomic sciences. 2010-12-25 Johnson TJ, Thorsness JL, Anderson CP, Lynne AM, Foley SL, Han J, Fricke WF, McDermott PF, White DG, Khatri M, Stell AL, Flores C, Singer RS. Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky, PloS one. 2010-12-22 Marques SM, Petushkov VN, Rodionova NS, da Silva JC. LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota, Journal of photochemistry and photobiology. B, Biology. 2010-12-21 Santos MJ, Fernandes D, Capela S, da Silva JC, ...
The partition system of multidrug resistance plasmid TP228 includes a novel protein that epitomizes an evolutionarily distinct...
The segregational stability of bacterial, low-copy-number plasmids is promoted primarily by active partition. The plasmid-specified components of the prototypical P1 plasmid partition system consist of two proteins, ParA (44.3 kDa) and ParB (38.5 kDa), which, in conjunction with integration host fac …
Bacteriocins | Riley Lab
The Role of Bacteriocins in Mediating Bacterial Competitive Interactions. Explaining the coexistence of competing species is a major challenge in community ecology. In bacterial systems, competition is often driven by the production of bacteriocins, which are narrow-spectrum proteinaceous toxins that serve to kill closely related species, providing the producer better access to limited resources. Bacteriocin producers have been shown to competitively exclude sensitive, nonproducing strains. However, the dynamics between bacteriocin producers, each lethal to its competitor, are largely unknown. In this study, we used in vitro, in vivo and in silico models to study competitive interactions between bacteriocin producers. Two Escherichia coli strains were generated, each carrying a DNA-degrading bacteriocin (colicins E2 and E7). Using reporter-gene assays, we showed that each DNase bacteriocin is not only lethal to its opponent but, at lower doses, can also induce the expression of its opponents ...
Plasmids: Properties, Types and Functions - Learn Microbiology Online
Individual bacterial cells may contain several different types of plasmids and in some cases more than 10 at a time. Plasmids are generally isolated from the bacterial cells in the supercoiled configuration. So far, thousands of different types of plasmids have been isolated. More than 300 different types of naturally occurring plasmids have been isolated from E.coli alone. Though, plasmids are not considered as part of the cells genome, when a bacterial cell divides each daughter cells receives a copy of each plasmid. Plasmids can also be transferred from one bacterial cell to another by the process called conjugation. Plasmids that govern their own transfer by conjugation are called conjugative plasmids but not all plasmids are conjugative.. ...
All Plasmids Achievement in BioShock 2 (RU) (PC)
All Plasmids Achievement in BioShock 2 (RU) (PC): Found or purchased all 11 basic Plasmid types - worth 20 GamerScore. Find guides to this achievement here.
Buy BOSCH KIL82AF30G Integrated Tall Fridge | Free Delivery | Currys
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Do I submit my plasmids as DNA or within bacteria? - Addgene Help Center
Plasmids can be submitted in either form. For DNA, aliquot 15 µL of DNA into a 1.5 mL microfuge tube (at a concentration of 0.1 -1µg/µL)...
Etmopterus - Viquipèdia, lenciclopèdia lliure
Günther, A., 1880. Report on the shore fishes procured during the voyage of H. M. S. Challenger in the years 1873-1876. A Report on the scientific results of the voyage of H. M. S. Challenger during the years 1873-1876. Zoology. Rept. Challenger Shore Fishes v. 1 (pt 6): 1-82, Pls. 1-32. ...
Definition : Molecular assay reagents intended to identify mutations in the tumor protein p53 (TP53) gene, located at chromosome 17p13.1, which encodes for a protein that acts as a tumor suppressor and induces cellular apoptosis. This inherited genetic mutation and/or genetic anomalies have been identified in patients with many types of cancers.. Entry Terms : 6-Mercaptopurine Inactivation Gene Mutation Reagents , Thiopurine Methyltransferase High Activity Gene Mutation Reagents , TPMT Gene Mutation Detection Reagents , Reagents, Molecular Assay, Gene Anomaly, Mutation, TPMT. UMDC code : 24995 ...
2N1T | Genus
chains in the Genus database with same CATH superfamily 5CCH A; 4WY4 A; 1URQ D; 5KJ7 C; 3HD7 B; 5KJ7 D; 4W80 A; 3RK3 B; 2N1T C; 3HD7 D; 3RK3 C; 2YMY A; 2M8R A; 3RK3 D; 1SFC A; 4W7Y A; 2HFE D; 1GL2 B; 1L4A D; 1SFC B; 2KOG A; 1GL2 C; 1GL2 D; 1SFC C; 1SFC D; 2N1T B; 1N7S A; 1JTH B; 3M0D C; 2N1T D; 1NHL A; 4WY4 B; 3KYQ A; 3B5N A; 3VOP A; 5CCH C; 4WY4 C; 2XDJ A; 5CCH D; 4WY4 D; 1N7S C; 3B5N B; 2PP6 A; 3RL0 B; 3PP5 A; 3B5N C; 3RL0 C; 3B5N D; 3RL0 D; 3TSI A; 1KIL A; 5CCG A; 4OH8 B; 1URQ A; 5KJ7 A; 1N7S B; 3RK2 C; 2NPS A; 1KIL C; 2HG5 D; 3HD7 A; 5CCG C; 1N7S D; 1URQ C; 3P8C E; 2NPS B; 2WZ7 A; 2H8P D; 1L4A A; 2NPS C; 2NPS D; 3HD7 C; 3HTK B; 1GL2 A; 3RK2 B; 4N78 E; 1KIL B; 1L4A B; 3RK2 D; 4W7Z A; 1L4A C; 2N1T A; 4LGD E; 1KIL D; 1JTH A; 1T3J A; 4JF7 A; 1URQ B; 5CCG D; 1HVV A; 1XTG B; #chains in the Genus database with same CATH topology 1KTM A; 1Q86 Q; 4I1L A; 1RF1 C; 1EZV D; 3AE9 C; 3BAT A; 2QSI A; 3V5B A; 3A0H E; 3A3Y B; 3AE1 C; 1HBW A; 5AVS B; 3TYY A; 1SQP K; 1YHQ P; 2XV5 A; 2G3A A; 1KQS O; 1NO4 A; 4JQ0 ...
3P8C | Genus
chains in the Genus database with same CATH superfamily 4WY4 D; 2HG5 D; 5KJ7 C; 4W7Z A; 3RL0 C; 3RK3 C; 5CCH A; 2PP6 A; 1SFC C; 3M0D C; 1JTH B; 3KYQ A; 3RK2 B; 1GL2 B; 3VOP A; 2M8R A; 2N1T A; 5CCG D; 3RL0 B; 5CCH D; 1N7S A; 1L4A C; 3RK2 D; 3B5N D; 2WZ7 A; 1GL2 C; 3HD7 A; 1N7S B; 1URQ A; 2YMY A; 1KIL C; 3B5N B; 1GL2 D; 5CCH C; 2NPS A; 1SFC A; 3TSI A; 1L4A D; 3RL0 D; 3B5N A; 5CCG C; 1NHL A; 4WY4 A; 1N7S D; 3PP5 A; 4WY4 C; 1L4A A; 1URQ C; 2HFE D; 1L4A B; 1JTH A; 5KJ7 A; 2N1T B; 1T3J A; 1KIL D; 2XDJ A; 2KOG A; 3P8C E; 1HVV A; 2N1T D; 2NPS D; 3HD7 B; 1GL2 A; 4W7Y A; 2N1T C; 4LGD E; 4OH8 B; 3RK3 B; 1KIL A; 3HD7 D; 2NPS B; 1SFC B; 3HD7 C; 4JF7 A; 3RK2 C; 3HTK B; 4W80 A; 1KIL B; 3B5N C; 5CCG A; 1SFC D; 1URQ B; 2NPS C; 1XTG B; 3RK3 D; 1URQ D; 4N78 E; 1N7S C; 4WY4 B; 5KJ7 D; 2H8P D; #chains in the Genus database with same CATH topology 1RF1 C; 1SQP D; 2L91 A; 2Y9Y A; 5AW2 B; 2HG5 D; 5KJ7 C; 1M1J B; 1YQ3 C; 2XNX A; 3L70 D; 1OWA A; 2KI7 B; 2IZ0 A; 1AQT A; 4GOF A; 2KXP B; 2R5D A; 4H44 B; 4JQ0 D; 3KQG A; 3MZW ...
Puteshestvie V Yuzhnuyu Rossiyu I Krym: cherez Vengriyu, Valahiyu i Moldaviyu, sovershennoe v 1837 g
Trouvez tous les livres de Demidov,A. N. - Puteshestvie V Yuzhnuyu Rossiyu I Krym: cherez Vengriyu, Valahiyu i Moldaviyu, sovershennoe v 1837 g. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 542414697X
9781145892880 - Oxonian - Confessions of an Oxonian [Ed. by T. Little].
Trouvez tous les livres de Oxonian - Confessions of an Oxonian [Ed. by T. Little].. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9781145892880
CORTU - Overview: Cortisol, Free, 24 Hour, Urine
CORTU : Preferred screening test for Cushing syndrome Diagnosis of pseudo-hyperaldosteronism due to excessive licorice consumption Test may not be useful in the evaluation of adrenal insufficiency
Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor binding. - Oxford...
Binding of enzymatic E colicins to the vitamin B12 receptor, BtuB, is the first stage in a cascade of events that culminate in the translocation of the cytotoxic nuclease into the Escherichia coli cytoplasm and release of its tightly bound immunity protein. A dogma of colicin biology is that the toxin coiled-coil connecting its functional domains must unfold or unfurl to span the periplasm, with recent reports claiming this reaction is initiated by receptor binding. We report isothermal titration calorimetry data of BtuB binding the endonuclease toxin ColE9 and a disulfide form (ColE9S-S) where unfolding of the coiled-coil is prevented and, as a consequence, the toxin is biologically inactive. Contrary to expectation, the thermodynamics of receptor binding, characterized by large negative values for TDeltaS, are identical for the two colicins, arguing against any form of BtuB-induced unfolding. We go on to delineate key features of the colicin translocon that assembles at the cell surface after BtuB
SimPlot analysis.Similarity plots with plasmids R751, p | Open-i
SimPlot analysis.Similarity plots with plasmids R751, pBP136 and pB3 as reference plasmids. Each coloured plot corresponds to a specific plasmid depicted in the
Innovative Metal Technologies | imm cologne
Alles rund um den Aussteller Innovative Metal Technologies ✓ Produkte und Dienstleistungen ✓ Messestand ✓ Kontaktinformationen
Colicin E3 immunity protein superfamily
The SCOP classification for the Colicin E3 immunity protein superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
Hubnut , diety a nov trendy ve shazov n kil - iDNES.cz
Jak jsou nov trendy v hubnut a diet ch, co je zdrav a inn , co pom h ? Jak diety mohou naopak u kodit? Dozv te se na Ona.idnes.cz
Uncle Tupelo. No Depression. ROCKDELUX (2014)
Reedición (2014) de No Depression (1990), kilómetro cero de lo que acabarían siendo Wilco y Son Volt. Por David Morán. Escucha No Depression.
DNA Prep Frequently Asked Questions
Please send us 10-50ul of samples with the concentration of 50-100ng/μl.Please do not send us your plasmids in low volume since they tend to evaporate.. ...
Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis - de research portal van de...
PLASMID", ... Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin ... Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis. / Venema, K.; Abee, T.; Haandrikman, A. ... Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis. Applied and environmental microbiology ... Lactococcin B (LcnB) is a small, hydrophobic, positively charged bacteriocin produced by Lactococcus lactis subsp. cremoris 9B4 ...