The prevention of access by infecting organisms to the locus of potential infection.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)

The significance of cagA and vacA subtypes of Helicobacter pylori in the pathogenesis of inflammation and peptic ulceration. (1/7662)

AIMS: To assess the significance of cagA and vacA subtypes of Helicobacter pylori in relation to inflammation and density of bacterial colonisation in vivo within a dyspeptic UK population. METHODS: Dyspeptic patients who were Helicobacter pylori positive had antral samples taken for histology and culture. Gastroduodenal pathology was noted. The grade of bacterial density and inflammation was assessed using the Sydney system. Bacterial DNA was extracted and the vacA alleles and the cagA/gene typed using PCR. RESULTS: 120 patients were studied. There was high rate of cagA positive strains in this population. Bacterial density did not correlate with the presence of peptic ulceration. There was a significant association between cagA positive strains and increased inflammation and bacterial density. The vacA s1 type independently correlated with extensive chronic inflammation but there was no association with bacterial density. The vacA m type did not correlate with extent of inflammation or bacterial density. CONCLUSIONS: The results suggest that cagA is important in the pathogenesis of inflammation and peptic ulceration. These findings are in keeping with the hypothesis that cagA acts as a marker for a cag pathogenicity island which encodes several genes involved in inflammation. The vacA s1 allele correlates with inflammation independently of cagA, possibly through its enhanced ability to produce the vacuolating cytotoxin.  (+info)

Acinetobacter bacteremia in Hong Kong: prospective study and review. (2/7662)

The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised.  (+info)

Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications. (3/7662)

The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities.  (+info)

Risk factors for nosocomial bloodstream infections due to Acinetobacter baumannii: a case-control study of adult burn patients. (4/7662)

Risk factors for Acinetobacter baumannii bloodstream infection (BSI) were studied in patients with severe thermal injury in a burn intensive care unit where A. baumannii was endemic. Of 367 patients hospitalized for severe thermal injury during the study period, 29 patients with nosocomial A. baumannii BSI were identified (attack rate, 7.9%). Cases were compared with 58 matched controls without A. baumannii BSI. The overall mortality rate was 31% among cases and 14% among controls; only two deaths (7%) were considered directly related to A. baumannii BSI. Molecular typing of A. baumannii blood isolates by means of randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis revealed the presence of three different strain types. Multivariate analysis showed that female gender (P = .027), total body surface area burn of > 50% (P = .016), prior nosocomial colonization with A. baumannii at a distant site (P = .0002), and use of hydrotherapy (P = .037) were independently associated with the acquisition of A. baumannii BSI in burn patients. These data underscore the need for effective infection control measures for this emerging nosocomial problem.  (+info)

Molecular differentiation of Renibacterium salmoninarum isolates from worldwide locations. (5/7662)

Renibacterium salmoninarum is a genospecies that is an obligate pathogen of salmonid fish and is capable of intracellular survival. Conventional typing systems have failed to differentiate isolates of R. salmoninarum. We used two methods to assess the extent of molecular variation which was present in isolates from different geographic locations. In one analysis we investigated possible polymorphisms in a specific region of the genome, the intergenic spacer (ITS) region between the 16S and 23S rRNA genes. In the other analysis we analyzed differences throughout the genome by using randomly amplified polymorphic DNA (RAPD). We amplified the spacer region of 74 isolates by using PCR and performed a DNA sequence analysis with 14 geographically distinct samples. The results showed that the 16S-23S ribosomal DNA spacer region of R. salmoninarum is highly conserved and suggested that only a single copy of the rRNA operon is present in this slowly growing pathogen. DNA sequencing of the spacer region showed that it was the same length in all 14 isolates examined, and the same nucleotide sequence, sequevar 1, was obtained for 11 of these isolates. Two other sequevars were found. No tRNA genes were found. We found that RAPD analysis allows reproducible differentiation between isolates of R. salmoninarum obtained from different hosts and different geographic regions. By using RAPD analysis it was possible to differentiate between isolates with identical ITS sequences.  (+info)

Biodiversity of Lactococcus garvieae strains isolated from fish in Europe, Asia, and Australia. (6/7662)

Lactococcus garvieae (junior synonym, Enterococcus seriolicida) is a major pathogen of fish, producing fatal septicemia among fish species living in very diverse environments. The phenotypic traits of L. garvieae strains collected from three different continents (Asia, Europe, and Australia) indicated phenotypic heterogeneity. On the basis of the acidification of D-tagatose and sucrose, three biotypes were defined. DNA relatedness values and a specific PCR assay showed that all the biotypes belonged to the same genospecies, L. garvieae. All of the L. garvieae strains were serotyped as Lancefield group N. Ribotyping proved that one clone was found both in Japan, where it probably originated, and in Italy, where it was probably imported. PCR of environmental samples did not reveal the source of the contamination of the fish in Italy. Specific clones (ribotypes) were found in outbreaks in Spain and in Italy. The L. garvieae reference strain, isolated in the United Kingdom from a cow, belonged to a unique ribotype. L. garvieae is a rising zoonotic agent. The biotyping scheme, the ribotyping analysis, and the PCR assay described in this work allowed the proper identification of L. garvieae and the description of the origin and of the source of contamination of strains involved in outbreaks or in sporadic cases.  (+info)

Randomly amplified polymorphic DNA analysis of clinical and environmental isolates of Vibrio vulnificus and other vibrio species. (7/7662)

Vibrio vulnificus is an estuarine bacterium that is capable of causing a rapidly fatal infection in humans. A randomly amplified polymorphic DNA (RAPD) PCR protocol was developed for use in detecting V. vulnificus, as well as other members of the genus Vibrio. The resulting RAPD profiles were analyzed by using RFLPScan software. This RAPD method clearly differentiated between members of the genus Vibrio and between isolates of V. vulnificus. Each V. vulnificus strain produced a unique band pattern, indicating that the members of this species are genetically quite heterogeneous. All of the vibrios were found to have amplification products whose sizes were within four common molecular weight ranges, while the V. vulnificus strains had an additional two molecular weight range bands in common. All of the V. vulnificus strains isolated from clinical specimens produced an additional band that was only occasionally found in environmental strains; this suggests that, as is the case with the Kanagawa hemolysin of Vibrio parahaemolyticus, the presence of this band may be correlated with the ability of a strain to produce an infection in humans. In addition, band pattern differences were observed between encapsulated and nonencapsulated isogenic morphotypes of the same strain of V. vulnificus.  (+info)

Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp. (8/7662)

A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture.  (+info)

Asepsis is a state or practice of being free from infection or contamination, especially by pathogenic microorganisms. It is a set of procedures and practices used in medicine and healthcare to prevent infection and the spread of disease-causing microorganisms. Aseptic techniques include the use of sterile equipment, barriers, and environmental controls to prevent the introduction of microorganisms into a susceptible host.

There are two types of asepsis: medical and surgical. Medical asepsis involves practices that reduce the number of microorganisms in the environment, such as hand hygiene, use of personal protective equipment (PPE), and cleaning and disinfection of surfaces and equipment. Surgical asepsis is a more stringent form of asepsis that aims to create a sterile field during surgical procedures, using sterilized instruments, drapes, gowns, gloves, and other materials to prevent the introduction of microorganisms into the surgical site.

Maintaining aseptic techniques is critical in healthcare settings to prevent the transmission of infectious agents and protect patients from harm. Failure to follow aseptic practices can result in healthcare-associated infections (HAIs), which can cause significant morbidity, mortality, and increased healthcare costs.

An encyclopedia is a comprehensive reference work containing articles on various topics, usually arranged in alphabetical order. In the context of medicine, a medical encyclopedia is a collection of articles that provide information about a wide range of medical topics, including diseases and conditions, treatments, tests, procedures, and anatomy and physiology. Medical encyclopedias may be published in print or electronic formats and are often used as a starting point for researching medical topics. They can provide reliable and accurate information on medical subjects, making them useful resources for healthcare professionals, students, and patients alike. Some well-known examples of medical encyclopedias include the Merck Manual and the Stedman's Medical Dictionary.

This includes medical and laboratory techniques (such as with bacterial cultures). There are two types of asepsis - medical and ... "Medical Asepsis (Clean Technique)" (PDF). Indiana Department of Health. "Microbiology Techniques & Troubleshooting". Science ... The exact modes of infection depend on the types of surgery, but the most common bacteria that are responsible for SSIs are ... While all members of the surgical team should demonstrate good aseptic technique, it is the role of the scrub nurse or surgical ...
The technique uses many different bacterial strains in order to compare the different changes in the genetic material. The ... One technique entails cytogenetic assays using different mammalian cells. The types of aberrations detected in cells affected ... The benefits of this technique are that it is a fast and simple method and convenient for numerous substances. These techniques ... This technique is advantageous in that it detects low levels of DNA damage, requires only a very small number of cells, is ...
Sequence Typing is a technique that uses genomic information to establish evolutionary relationships between bacterial taxa. ... "Halobacterium noricense" at the Encyclopedia of Life LPSN Type strain of Halobacterium noricense at BacDive - the Bacterial ... "ATCC medium: 2185 Halobacterium NRC-1 medium". American Type Culture Collection (ATCC). Lim SK, Kim JY, Song HS, Kwon MS, Lee J ... When the samples were compared to the fosmid library, some were over 88% similar to other known halophilic bacterial species. ...
Most types of bacteria cannot change to the endospore form. Examples of bacterial species that can form endospores include ... Another staining technique for endospores is the Schaeffer-Fulton stain, which stains endospores green and bacterial bodies red ... While the rest of a bacterial cell may stain, the endospore is left colourless. To combat this, a special stain technique ... The main types within the cell are terminal, subterminal, and centrally placed endospores. Terminal endospores are seen at the ...
The type of medium in the agar plates is also important. Chromagar Candida is a differential medium that is used to identify ... Protist species used include Euglena gracilis (photosynthetic, green) and Physarum polycephalum (yellow-green). A technique ... called "bacteriography" involves selectively killing certain areas of a bacterial culture with radiation, in order to produce ...
Their use is based on the variable bowel bacterial flora associated with persons of different homeopathic constitutional types ... In 1930, at the age of 43, he decided to search for a new healing technique. He spent the spring and summer discovering and ... In this period, he developed seven bacterial nosodes known as the seven Bach nosodes. Their use has been mostly confined to ...
The basics of this technique involve the preparation of a primer that will be a complementary strand to a wild type bacterial ... When the bacterial plasmid is replicated, the mutated strand will be replicated as well. The same technique can be used to ... Although there are three different types of solid state enzyme-linked immunosorbent assays, all three types begin with the ... The technique was developed by Kary Mullis in 1983 while working for the Cetus Corporation. Mullis would go on to win the Nobel ...
... it has been shown that silver nano particles lower the bacterial count on devices used compared to old techniques. However, the ... Although it varies for every type of cell proposed, as their cell membrane composition varies greatly, It has been seen that in ... This technique is popular due to the ability to add a variety of functionality to the exposed silica surface. A number of ... Bacterial and fungal synthesis of nanoparticles is practical because bacteria and fungi are easy to handle and can be modified ...
In this situation, the bacterial profile modification technique, which injects bacteria into the high permeable zone to promote ... "Effects of bacterial cells and two types of extracellular polymers on bioclogging of sand columns". Journal of Hydrology. 535: ... It is a type of microbial enhanced oil recovery. Biofilm Hydraulic conductivity Landfill liner Microbial enhanced oil recovery ... In the extraction of petroleum, a technique of enhanced oil recovery is implemented to increase the amount of oil to be ...
Bacterial colonies can be counted after 24 to 48 hours depending on the type of bacteria. Counts are reported as colony forming ... units per 100 mL (cfu/100 mL). One technique for detecting indicator organisms is the use of chromogenic compounds, which are ... None of the types of indicator organisms that are currently in use fit all of these criteria perfectly, however, when cost is ... Indicator bacteria are types of bacteria used to detect and estimate the level of fecal contamination of water. They are not ...
The response is a classical example of delayed-type hypersensitivity reaction (DTH), a type IV of hypersensitivities. T cells ... A person who has been exposed to the bacteria is expected to mount an immune response in the skin containing the bacterial ... This intradermal injection is termed the Mantoux technique. ...
discuss several examples of types of studies in which RISA can be used. They cite several studies that have used this technique ... In this study, they were looking at a group of closely related bacterial types, all autotrophic β-proteobacterial ammonia ... Biodiversity, Environmental microbiology, Genetics techniques, Molecular biology techniques). ... The brightness of the fluorescently labeled primers correlates to how prevalent that bacterial type is in the community. The ...
In other words, PFGE is an advanced technique that enables scientists to gain a deeper understanding of bacterial genomes, even ... There are various types of PFGE methods, such as FIGE, TAFE, CHEF, OFAG, PHOGE and PACE, that are used for isolating and typing ... This method is particularly useful for the typing of bacterial isolates from clinical specimens, such as blood, urine, and ... So that Pulsed Field Gel Electrophoresis (PFGE) is a powerful technique used for analyzing bacterial genomes when traditional ...
This technique prevents a phenomenon known as "lysis from without", which is bacterial lysis induced by high multiplicity phage ... The bacterial strain is assigned a type based on its lysis pattern. Phage typing was used to trace the source of infectious ... Phages used for phage typing are generally isolated from the native habitats of the host bacterial strain. These may include ... CRAIGIE, JAMES; YEN, CHUN HUI (1938). "The Demonstration of Types of B. Typhosus by Means of Preparations of Type II Vi Phage: ...
2003) observed inhibiting bacterial growth with up to 83% of the efficacy of TBT oxide. Current research also aims to produce ... Different system types can be used to yield different biogenic products. In the field of paleochemotaxonomy the presence of ... By pairing these techniques with biochemical engineering design, algae and their biogenic substances can be produced on a large ... TISTR 8208 in a Seaweed-Type Bioreactor". In Finkelstein M, Davison BH (eds.). Biotechnology for Fuels and Chemicals. pp. 249- ...
... (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro ... Vaccine delivery is a very common application of bacterial surface display. There are two types of live bacterial vaccines that ... Bacterial display combined with FACS also has the advantage that it is a real-time technique. Cell display systems were first ... Bacterial display systems were first introduced by Freudl et al. and Charbit et al. in 1986, when they used bacterial surface ...
... the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as C ... As a result of suppression of healthy bacteria, via a loss of bacterial food source, prolonged use of an elemental diet ... Taking non-toxic types of C. difficile after an infection has promising results with respect to preventing future infections. ... C. difficile is one such type of bacterium. In addition to proliferating in the bowel, C. difficile also produces toxins. ...
This new technique, identifying bexB in clinical isolates, is now used by the Centers for Disease Control to type H. influenzae ... Gilsdorf, J. R. (1 November 1977). "Bacterial meningitis in southwestern Alaska". American Journal of Epidemiology. 106 (5): ... This observation led to clinical trials of H. influenzae type b vaccines in native Alaskan children, who exhibited a rate of ... Granoff, D. M.; Gilsdorf, J.; Gessert, C. E.; Lowe, L. (January 1980). "Haemophilus influenzae type b in a day care center: ...
... where he worked to develop molecular biology techniques for bacterial strain screening, microbial identification, and typing of ... Horvath explored sections in the bacterial genome with clustered regularly interspaced short palindromic repeats, both for ... Early work was stimulated by with the aim of improving the durability of bacterial starter cultures to improve the manufacture ... their utility in differentiating between strains, and because of their role in the bacterial immune system. As this prokaryotic ...
ISBN 978-1-84593-479-8. {{cite book}}: ,first1= has generic name (help) Type strain of Xenorhabdus ehlersii at BacDive - the ... phylogeny and bacterial symbionts. Leiden: Brill. ISBN 978-90-474-2239-6. {{cite book}}: ,last2= has generic name (help) ... Insect pathogens molecular approaches and techniques. Wallingford, UK: CABI. ... Bacterial Diversity Metadatabase v t e (CS1 errors: generic name, Articles with short description, Short description is ...
... bacterial typing techniques MeSH E05.200.875.150.125.150 - bacteriophage typing MeSH E05.200.875.150.125.765 - ribotyping MeSH ... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... mycological typing techniques MeSH E05.200.875.837 - serial passage MeSH E05.200.875.950 - viral load MeSH E05.200.875.970 - ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ...
... a leading cause of bacterial meningitis and epiglottitis. The techniques he and his colleague Ronald Eby invented were later ... microbiologist best known for developing a vaccine that protects children from infections by Haemophilus influenzae type b (Hib ... The conjugate vaccine technique Anderson and his colleagues developed led to a Streptococcus pneumoniae (pneumococcus) vaccine ... "A lucky career in bacterial vaccines." (2012) Human Vaccines & Immunotherapeutics, vol 8, issue 4, pp. 420-422 Clark, Thornton ...
Among other things, this technique is used to isolate specific cell types (e.g., guard cells from leaves, pericycle cells from ... In the case of Gram-negative bacterial spheroplasts, for example, the peptidoglycan component of the cell wall has been removed ... This technique may be used to generate somatic hybrids in tissue culture.[citation needed] Additionally, protoplasts of plants ... Protoplasts can be generated by stripping the cell wall from plant, bacterial, or fungal cells by mechanical, chemical or ...
This type of asymmetry is usually seen in Bacilli and Clostridia. Reproductive asymmetry is classically linked to bacterial ... this idea has been overturned by novel technology and observation techniques. However, asymmetric bacteria remain difficult to ... In this type of asymmetrical growth, the daughter cell receives most of the new cell wall material. Bacillus subtilis ... Following the "old pole" of the cell wall material allows an observer to create a bacterial lineage. Bacteria exhibit three ...
However, the microbiome eventually returns to a state of equilibrium, even though the composition of bacterial types has ... By mapping the normal microbial make-up of healthy humans using genome sequencing techniques, the researchers of the HMP have ... Other studies using the HMP data and techniques include role of microbiome in various diseases in the digestive tract, skin, ... It is estimated that bacterial protein-coding genes are 360 times more abundant than human genes.[citation needed] Microbial ...
... (MLST) is a technique in molecular biology for the typing of multiple loci, using DNA sequences of ... Earlier serological typing approaches had been established for differentiating bacterial isolates, but immunological typing has ... Spratt, Brian G (1999). "Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing ... Until recently, with the application of MLST technique, Campylobacter typing has achieved a great success and added onto the ...
... such as the application of a bacterial toxin, submersion in a weak acid, or physical trauma. The technique gained prominence in ... She repeated the experiment with other cell types including brain, skin, and muscle cells with the same result. Initially ... After modifying the technique, Obokata was able to show that white blood cells from newborn mice could be transformed into ... The technique for producing STAP cells was subsequently studied by Obokata at the Brigham and Women's Hospital (BWH), while she ...
This technique allows for replicate spots of one bacterial type to be tested or spots of different bacteria so that the MIC of ... Necessary controls include a control plate that does not receive any antibiotics and bacterial spread plates demonstrating that ... The lowest concentration of antibiotics that prevented bacterial growth is considered to be the minimum inhibitory ... the plates are examined to determine if bacterial growth has occurred in the inoculated spots. ...
In 2009, researchers at the University of Vermont unveiled a new type of tap that prevents backflow of sap into the tree, ... Improvements in tubing and vacuum pumps, new filtering techniques, "supercharged" preheaters, and better storage containers ... reducing bacterial contamination and preventing the tree from attempting to heal the bore hole. Experiments show that it may be ... but these techniques were not widely used. Heating methods also diversified: modern producers use wood, oil, natural gas, ...
Proper sampling technique is essential to avoid potential pre-analytical errors. For example, different types of samples must ... to preserve the virus and prevent bacterial or fungal growth. Sometimes multiple sites may also be sampled.[citation needed] ... The type of sample sent to the laboratory often depends on the type of viral infection being diagnosed and the test required. ... However, electron microscopy is highly versatile due to its ability to analyze any type of sample and identify any type of ...
... typical products are Moscow-type and Russian-type salamis made from pork and beef meat. A particular feature of Moscow-type ... Minor differences in the formulation of the meat or production techniques give rise to the various kinds of salami across ... lactic acid bacterial starter culture.[citation needed] This mixture is then inserted into casings of the desired size. To ... Similar types of sausages are found in the Middle East, where various meats such as beef, lamb, and mutton are used; or in ...
With treatment, most types of bacterial pneumonia will stabilize in 3-6 days. It often takes a few weeks before most symptoms ... among other techniques. Mycoplasma, Legionella, Streptococcus, and Chlamydia can also be detected using PCR techniques on ... Viruses also make the body more susceptible to bacterial infections; in this way, bacterial pneumonia can occur at the same ... as there is no definitive test able to distinguish between bacterial and non-bacterial cause. The overall impression of a ...
... criteria for bacterial strain typing. J Clin Microbiol. 1995;33:2233-9.PubMedGoogle Scholar ... Enterococci were isolated from infected patients by classical microbiologic techniques (3). Identification at the species level ... D-type glycopeptide resistant Enterococcus faecium BM4339. Antimicrob Agents Chemother. 1997;41:2016-8.PubMedGoogle Scholar ... VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis. Antimicrob Agents Chemother. 1999;43:2161-4. ...
Learn the three types of reflexology, as well as its pros and cons, benefits, treating conditions, and how it works. ... Get over colds and bacterial infections. *Clear up sinus issues. *Recover from back problems ... 3 types of reflexology. *Generic reflexology: A relaxation therapy involving a special foot massage to relax, energize, and ... People practicing this technique are called reflexologists.. Reflexology rests on the ancient Chinese belief in qi (chee or ...
Bacterial Typing Techniques * Base Composition * Comoros * DNA, Bacterial / genetics * Genes, Bacterial * Humans ... is proposed, with 200901116(T) ( = CIP 110703(T) = DSM 28999(T)) as the type strain. ...
This includes medical and laboratory techniques (such as with bacterial cultures). There are two types of asepsis - medical and ... "Medical Asepsis (Clean Technique)" (PDF). Indiana Department of Health. "Microbiology Techniques & Troubleshooting". Science ... The exact modes of infection depend on the types of surgery, but the most common bacteria that are responsible for SSIs are ... While all members of the surgical team should demonstrate good aseptic technique, it is the role of the scrub nurse or surgical ...
Categories: Bacterial Typing Techniques Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
Publication types * Evaluation Study * Multicenter Study MeSH terms * Bacterial Infections / diagnosis* * C-Reactive Protein / ... An SBI was defined as isolation of a bacterial pathogen from urine, blood, cerebrospinal fluid or stools, an IBI as isolation ... Diagnostic performance of the lab-score in predicting severe and invasive bacterial infections in well-appearing young febrile ... We aimed to assess the Lab-score usefulness in predicting SBI, especially invasive bacterial infections (IBIs), in well- ...
Pericardial fluid Gram stain is a method of staining a sample of fluid taken from the pericardium to diagnose a bacterial ... The Gram stain method is one of the most commonly used techniques to rapidly identify the type of bacteria causing the ... Pericardial fluid Gram stain is a method of staining a sample of fluid taken from the pericardium to diagnose a bacterial ... Blood tests and bacterial culture can help identify the specific organism causing the infection. ...
Bieber D, Ramer SW, Wu CY, Murray WJ, Tobe T, Fernandez R, et al. Type IV pili, transient bacterial aggregates, and virulence ... Using this labeling technique, we have recently demonstrated that the retraction of DNA-bound type IV competence pili is ... Type IV pilus structure and bacterial pathogenicity. Nat Rev Microbiol. 2004;2(5):363-78. doi: 10.1038/nrmicro885. PubMed PMID ... The facultative bacterial pathogen Vibrio cholerae uses a type IV competence pilus for DNA uptake during natural transformation ...
As a matter of fact, molecular techniques now exist to screen livers for the presence of any type of bacteria. So far, these ... Recurrent bacterial infections of the urine occur in some women with PBC. These infections may be without symptoms or cause a ... One type (called cholesterol gallstones) contains mostly cholesterol, and is by far the most common type of gallstone found in ... Auto means self.) Type 1 diabetes is one example of an autoimmune disease in which some type of transient infection (one that ...
Databases were established for identification of bacterial species and protein types within species and standard 2-dimensional ... For wide use, standardization of techniques is of paramount importance.. A standard procedure has been developed for the ... 1-dimensional and 2-dimensional electrophoretic techniques provide powerful tools for the analysis of complex protein mixtures ... dimensional gel analysis namely PDQUEST and BioImage were implemented and databases were established for the studied cell types ...
Several bacterial secretion systems give the bacteria an advantage allowing them to form for example biofilms. The biofilm ... The TAD pili transport system represents a subtype of the Type IV filament secretion systems. The TAD system has been found in ... He focuses on in situ cultivation, a technique involving growing bacteria in their native habitat, and adapting it into high- ... His work focuses on bacterial dynamin-like protein and PspA, both of which have eukaryotic homologues. Thus, these proteins ...
Effectiveness of the deryng and clevenger-type apparatus in isolation of various types of components of essential oil from the ... Poisoning: from specific toxic agents to novel rapid and simplified techniques for analysis London: IntechOpen. ... Effectiveness of the deryng and clevenger-type apparatus in isolation of various types of components of essential oil from the ... Impact of bacterial and somatic cells content on quality fresh milk in small-scale dairy farms in Kosovo. Food and Nutrition ...
Cell Culture Techniques; Cell Death; Cell Line; Cell model; cell type; Cells; cerivastatin; Chemicals; Coculture Techniques; ... Address; Animal Model; Animal Testing; Animals; Bacterial Luciferases; base; Biological Assay; Biological Availability; ... Biological Markers; Bioluminescence; Brain Stem; Cell Culture System; Cell Culture Techniques; Cell Line; cell type; Cells; ... type=hidden> Still Need Help?. If you need further assistance, send us an e-mail and we will assist you in resetting your ...
Manalis and colleagues showed that their microfluidic technique can measure the growth rates of various cell types, including ... at a resolution of 0.2 pg/h for mammalian cells and 0.02 pg/h for bacterial cells. "The cells mass accumulation rate provides ... "We now have techniques that help us work better with individual cells," asserted Dr. Ståhlberg. "And we need to become even ... "One thing about this type of cancer is that for years, people have been seeking the cell of origin," explained Dr. Li. For ...
Treatment Types. Most people will respond positively to a simple antibiotic ointment. Depending on the severity of your ... Depending on your family history, your doctor may take a swab to check for specific bacterial infection. You may also have a ... And our providers techniques are honed on some of the toughest cases in Virginia. ... You might have both types, or just one. The causes and treatment options are the same for both. ...
Limitations of techniques. CT continues to be used as a screening tool in the metastatic workup of many cancer patients but is ... The estimated sensitivity of MRI in the detection of LC is 34-71%, although this includes all tumor types. Separating LC into ... MRI findings in LC are nonspecific, and may be similar to those in patients with bacterial or fungal meningitis, leptomeningeal ... Flow studies using cardiac-gated phase contrast techniques can be used to display and quantitate CSF flow in the head and spine ...
Bacterial diversity and community composition from seasurface to subseafloor. Authors: Emily A. Walsh, John B. Kirkpatrick, ... During my C-DEBI postdoctoral tenure, I successfully developed the first genetic tools and techniques to transform and express ... Those results shed a more detailed light on what it means for a bacterial cell to grow in deep sea conditions and will provide ... Separate incubations using marine bacterial isolates (Vibrio sp. and Pseudoalteromonas sp.) and sterilized sediment under oxic ...
... is considered the reference technique for typing most bacterial species (5); but this technique is costly, labor-intensive and ... Various molecular methods have been used for bacterial typing and the chromosomal restriction patterns by pulsed field gel ... and is likely to replace current molecular typing techniques in the future (5,8). ... Typing of isolates is useful during outbreaks to determine the prevalence and mode of spread of strains and to identify ...
Traditionally, it has been typed on the basis of its phenotypic characteristics but this technique may lack discriminatory ... targeting highly-conserved repetitive sequence elements in the bacterial genome have shown considerable potential as DNA typing ... ERIC-PCR typing. ERIC-PCR typing was performed as reported previously [21] using PCR-GOLD Master-Mix Beads (Bioron, The Enzyme ... On calculating the discriminatory index of both typing methods (antibiotyping and ERIC), we found that ERIC typing gave a ...
Bacterial Proteins/analysis/genetics, Bacterial Typing Techniques, Belgium, DNA Fingerprinting, DNA, Bacterial, Fermentation, ... Bacterial Typing Techniques, Beer/microbiology, Biodiversity, Carbohydrate Metabolism, Colony Count, Microbial, Fermentation, ... LR: 20140904; JID: 7605801; 0 (Bacterial Proteins); 0 (DNA, Bacterial); EC (Phenylalanine-tRNA Ligase); OID: NLM: ... The amount leached depended on the type of can, but not on the amount of BADGE leached from the cans. Considerably more BPA ...
Ref Type: Catalog.. 6. N.Pallua, P.C.Fuchs, et al. (1999), A new technique for quantitative bacterial assessment on burn wounds ...
... it was most commonly bacterial and typically Clostridium spp or Salmonella spp, which could be useful information for ... or bacterial (167/710 [23.5%]). The most common bacteria responsible for FIILs were Clostridium spp and Salmonella spp. ... for the detection of C perfringens and C difficile toxins and the use of molecular techniques to detect and type bacterial ... For instance, age is a risk factor for some bacterial diseases, such as necrotic enteritis caused by C perfringens type C that ...
Techniques currently used for assessment of bacterial count or growth are time-consuming, offer low throughput, or they are ... Comparing the killing effect of wild type and NADPH oxidase-deficient murine neutrophils presents an example of detection of a ... Our proposed technique is based on flow cytometry (FC) optimized for detection of small particles and on fluorescently labeled ... Strong correlation was obtained between the results of the different techniques, but the reproducibility of the FC-based test ...
... of the epithelial mucosal barrier provide an effective first line defense against invading pathogens including bacterial ... of the epithelial mucosal barrier provide an effective first line defense against invading pathogens including bacterial ... Association between acquisition of herpes simplex virus type 2 in women and bacterial vaginosis. Clin Infect Dis. (2003) 37:319 ... These high-resolution techniques have enabled the classification of the vaginal microbiota into five community state types ( ...
pruni inoculation techniques to screen for bacterial spot resistance in apricot. Journal of Plant Pathology. 94, S1.91-S1.96.. ... A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS. ... II International Workshop on Bacterial Diseases of Stone Fruits and Nuts.. II International Workshop on Bacterial Diseases of ... First report of bacterial leaf spot of Hydrangea in retail nurseries in Belgium caused by strains assigned to a new Xanthomonas ...
... and immunofluorescence are applied based on the sample type and method of detection used in the immunostaining technique. ... 24 Bacterial pathogens such as, Bartonella quintana, Yersinia pestis, Treponema pallidum, Chlamydia trachomatis; viral ... Her research has involved using molecular, cell and computational biology tools and techniques to understand the pathological ... Immunohistochemistry: Tips and Techniques To Save Time, Money, and Tissue. READ MORE ...
... makes patients exclusive use also can have fine prevention bacterial infection, has reduced the condition that the infection ... and the patient can be used alone to have good bacterial infection prevention, the aseptic technique of this device of being ... can only just in specific place when using this type of product, and this device passes through the fungi-proofing effect of ... 206010060945 Bacterial infection Diseases 0.000 abstract description 5 * 230000000249 desinfective Effects 0.000 abstract ...
Type. Company. Catalog Number. Comments. API-3000 Triple Quadrupole. Instrument. SCIEX. Purchased with Analyst 1.4.2 (Applied ... 8 In the past several decades other atmospheric pressure ionization MS techniques have been applied to the characterization of ... Figure 1. Schematic for SESI-MS analysis of bacterial volatiles. The headspace of the bacterial culture is displaced by CO2 (1 ... We are expanding the use of SESI-MS to the detection of bacterial volatiles as a method for bacterial identification and ...
  • Computer analysis of 1-dimensional and 2-dimensional protein patterns to establish databases of pathogenic bacteria, biotechnologically important bacteria and eucaryotic cells provides methods for screening and identifying bacterial strains, recognizing new groups, and characterizing human and animal disease related protein abnormalities. (
  • Typing of isolates is useful during outbreaks to determine the prevalence and mode of spread of strains and to identify reservoirs and carriers. (
  • An important limitation of current infection control methodology is that the available bacterial typing methods cannot distinguish between different strains of MRSA. (
  • Current clinical methods to make links between related strains compare the pattern of bacterial susceptibility to a profile of antibiotics. (
  • Adherence to collagen type I was shown of 20% of the clinical strains and 6% of the strains of animal origin. (
  • A unique method to screen large-scale libraries for industrially useful bacterial strains was recently developed by Tokyo Tech researchers. (
  • Nonetheless, the traditional method of genetically engineering bacterial strains for high protein production is extremely time consuming. (
  • The study was led by Associate Professor Tetsuya Kitaguchi from Tokyo Institute of Technology (Tokyo Tech), Japan, and was conducted in collaboration with Ajinomoto Co., Inc. The innovative method, which combines microfluidics and versatile biosensing to quickly identify enhanced bacterial strains that produce the highest amount of a desired protein, is reported in their study published in the journal Small on April 24, 2023. (
  • The researchers put their method to the test by screening a huge library of bacterial strains created to produce FGF9, a human cytokine, and subjected to circumstances that cause random mutations. (
  • The "Lab-score" combining C-reactive protein, procalcitonin and urine dipstick results has recently been derived and validated as an accurate tool for predicting severe bacterial infections (SBIs) in children with fever without source. (
  • Antimicrobial resistance (AMR) in bacterial infections has emerged as a significant global health concern. (
  • Gram-negative bacterial infections, such as those caused by Pseudomonas aeruginosa , pose significant challenges in treatment due to their complicated structure. (
  • Rachel Wheatley receives funding from the Innovative Medicines Initiative Joint Undertaking under COMBACTE-MAGNET (Combatting Bacterial Resistance in Europe-Molecules against Gram-negative Infections) and COMBACTE-NET (Combatting Bacterial Resistance in Europe-Networks), resources of which are composed of financial contribution from the European Union's Seventh Framework Program and EFPIA companies' in kind contribution. (
  • The high incidence of bacterial respiratory infections has led to a focus on evaluating the human respiratory microbiome. (
  • Although considered the gold-standard for diagnosing bacterial infections this process is time-consuming, taking several days to produce the results, and requires specialist equipment and expert laboratory personnel. (
  • This will decrease the time taken to identify and deliver beneficial treatments to patients, and therefore improve the treatment of bacterial infections. (
  • Every lesional, itchy animal should be evaluated for secondary bacterial and Malassezia skin and ear infections , using standard procedures (i.e., impression smears, tape preparations). (
  • Acetate tapes are only to be used when yeast infections are suspected as the background material on the Scotch tape makes bacterial evaluation difficult, especially cocci. (
  • Bacterial infections may develop slowly and tend to recur (chronic bacterial prostatitis) or develop rapidly (acute bacterial prostatitis). (
  • Pericardial fluid Gram stain is a method of staining a sample of fluid taken from the pericardium to diagnose a bacterial infection. (
  • The Gram stain method is one of the most commonly used techniques to rapidly identify the type of bacteria causing the infection. (
  • Blood tests and bacterial culture can help identify the specific organism causing the infection. (
  • Depending on your family history, your doctor may take a swab to check for specific bacterial infection. (
  • Secondary bacterial infection is common, and lesions may cause increased pain and disability and fatal septicaemia (severe infection involving the entire human system) if untreated. (
  • At the American Society for Microbiology (ASM) Microbe 2018 meeting, Bruker announces improved solutions for microbial strain typing, hospital hygiene and infection control. (
  • The bacterial infection associated with atypical pneumonia mostly occurs in the upper respiratory tract, but it may also affect the lower respiratory tract. (
  • In a new study released today in New England Journal of Medicine , researchers demonstrate that whole-genome sequencing can provide clinically relevant data on bacterial transmission within a timescale that can influence infection control and patient management. (
  • Fast and accurate detection of bacterial transmission is crucial to better control of healthcare-associated infection. (
  • Primary hypertension isn't caused by a virus or a bacterial infection, but rather is almost entirely related to lifestyle factors. (
  • In our recently published study , we investigated the bacterial population in the lungs of an intensive care patient who had a lung infection caused by a common type of bacteria, Pseudomonas aeruginosa . (
  • We used a number of techniques and experiments that measured bacterial growth and any changes in antibiotic resistance that happened during the infection. (
  • However, the bacterial infection reappeared around 11 days after it was last detected - and with antibiotic resistant mutants. (
  • The Department of "Computational Biology for Infection Research" studies the human microbiome, viral and bacterial pathogens, and human cell lineages within individual patients by analysis of large-scale biological and epidemiological data sets with computational techniques. (
  • Both papers--one published online July 28, 2005 in Science Express and one in the August issue of Infection and Immunity --focus on aspects of the type-III pathway, a molecular syringe that Yersinia pestis , the bacterium that may have killed more people throughout history than any other infectious disease, uses to disable its host's immune system. (
  • The technique can be used to identify whether the bacteria causing an infection such as MRSA is resistant to certain drugs and antibiotics, and therefore identify treatments most likely to be effective. (
  • Currently, when a patient presents with a bacterial infection, the infection is diagnosed by taking a sample from the patient, growing bacteria from it and then using genetic sequencing to identify the type of bacteria present and the presence of markers of resistance. (
  • The cause is sometimes a bacterial infection. (
  • Bacterial infection is treated with antibiotics. (
  • Some people develop chronic prostatitis in the absence of bacterial infection. (
  • Occasionally, prostatitis without bacterial infection causes inflammation but no symptoms. (
  • Molecular typing by pulsed field gel electrophoresis showed that all isolates were closely related. (
  • Comparison ("typing" or "fingerprinting") of epidemiologically related isolates helps to determine whether these organisms are related or not and thus essential to confirm the existence of an outbreak. (
  • P. aeruginosa isolates from patients' and staff hands and environmental samples were typed using antibiotyping and ERIC-PCR. (
  • Current laboratory techniques often cannot distinguish between MRSA isolates. (
  • In an analysis of 292 bacterial isolates, the device was able to detect between 20 to 100 genome types. (
  • In total, 346 bacterial isolates were characterized based on phenotypic and sequencing-based molecular methods. (
  • Until the late 19th century, physicians rejected the connection between Louis Pasteur's germ theory that bacteria caused diseases and antiseptic techniques. (
  • Type IV pili are ubiquitous surface appendages in Gram-negative bacteria that promote diverse activities including attachment, virulence, biofilm formation, horizontal gene transfer, and twitching motility [ 1 - 5 ]. (
  • For bacterial mycetoma (actinomycetoma) treatment relies on long-term treatment with a combination of antibiotics, tailored to the type of bacteria involved. (
  • Lactobacilli and their antimicrobial and anti-inflammatory products along with components of the epithelial mucosal barrier provide an effective first line defense against invading pathogens including bacterial vaginosis, aerobic vaginitis-associated bacteria, viruses, fungi and protozoa. (
  • Briefly, tagRNA-seq makes it possible to identify the 5' ends of RNAs in bacteria and directly probe for their type, primary or processed, by ligating short RNA sequences, the tags, to the beginnings of RNA molecules. (
  • For example, the data in the sheet "DIwater_Run1" shows pressure reading across a membrane filter when DI water is infused in, while the sheet "Bacteria_Run1" shows the pressure reading across the same filter when the bacterial solution is infused in. (
  • Remarkably, the presence of potential pathogenic bacteria in the nasopharynx of healthy children can predispose them to infectious diseases, and also frequent exposure to human respiratory bacterial pathogens are further risk factors. (
  • This technique enabled the team to identify the cell types targeted by the bacteria. (
  • A new technique developed by scientists in the Physical Sciences for Health Centre at the University of Birmingham can identify the resistance profile of bacteria in a much shorter time than traditional diagnostic methods. (
  • Author Summary Almost all bacterial species use thin surface appendages called pili to interact with their environments. (
  • Databases were established for identification of bacterial species and protein types within species and standard 2-dimensional methodology was applied to human, murine, bacterial and streptomycete cells. (
  • We have demonstrated that SESI-MS volatile fingerprinting, combined with a statistical analysis method, can be used to differentiate bacterial genera, species, and mixed cultures in a variety of growth media. (
  • Microbiota composition was analyzed by IS-pro, a PCR technique based on species-specific differences in the 16S-23S interspace region of the bacterial ribosomal DNA. (
  • Small intestinal bacterial overgrowth (SIBO) consists of increased and/or abnormal microbiota comprising different bacterial species derived from the colonic flora that ascend to small intestine segments and may be associated with a myriad of gastrointestinal symptoms and extraintestinal complications 1 1. (
  • combined with metagenomics has uncovered thousands of novel bacterial species directly from samples without isolation or culturing. (
  • We used the method to directly probe for transcription start and processing sites in two bacterial species, Escherichiacoli and Enterococcus faecalis. (
  • Bacterial identification was performed by phenotypic methods and in house developed PCR-based sequencing (the V1-V9 hypervariable region of the bacterial 16S ribosomal RNA gene). (
  • We are expanding the use of SESI-MS to the detection of bacterial volatiles as a method for bacterial identification and characterization 8 . (
  • This could provide more insight into the microbiota composition in different sample types and might contribute to the development of a less invasive biomarker for diagnosing and monitoring IBD. (
  • Microbiota profiles of the three different sample types were compared within the same patient (for each patient: fecal sample vs. mucosal biopsy, fecal sample vs. rectal swab, rectal swab vs. mucosal biopsy), and between different patients (for each patient: fecal sample of one patient vs. mucosal biopsies of all patients, fecal sample of one patient vs. rectal swabs of all patients, etcetera). (
  • For each patient, similarity of microbiota composition between two sample types was assessed by calculating the Pearson's correlation (expressed as R 2 ). (
  • We found a significantly higher correlation between the microbiota profiles of different sample types within the same patient, than between microbiota profiles of different sample types of different patients (median R 2 0.27-0.33 and 0.02-0.03 respectively, Figure 1). (
  • Microbiota composition in different sample types from the same patient were more similar to each other than to profiles from different patients. (
  • The anti-bacterial effect of different antibiotics and EOs (thyme, oregano, lemongrass, mint, and rosemary) was determined using the standard disc diffusion method. (
  • We report here the first isolation and characterization of a cluster of VanA-type glycopeptide-resistant E. faecium in a teaching hospital in Colombia. (
  • This protocol provides instructions for the rapid (within minutes) characterization of bacterial VOCs using SESI-MS. (
  • 8 Here we provide the steps for obtaining bacterial volatile fingerprints using SESI-MS, including the instrumental parameters that should be optimized to ensure robust bacterial identification and characterization. (
  • As a demonstration of the use of SESI-MS for the characterization of bacterial volatiles, E. coli K12 and P. aeruginosa PAO1 are cultured aerobically for 24 h in 50 mL LB-Lennox at 37 °C and the SESI-MS spectra of the headspace volatiles are collected in 2 minutes. (
  • Analysis time is highly dependent on the complexity of the sample type and the analysis mode itself-there is a faster mode for viral and bacterial identification only or a more comprehensive mode that will align to all sequences in GenBank, Chiu explained. (
  • This approach allows these antibiotics to be delivered into bacterial cells through a pathway that mimics iron uptake. (
  • These factors collectively impede the accumulation of antibiotics at the bacterial target site. (
  • Sideromycin is a novel type of antibiotic where the parent antibiotics or prodrug incorporates a siderophore molecule that utlises iron transport system for delivery. (
  • This also made the antibiotics more successful, as they work better when targeting small bacterial populations. (
  • In our hands, it showed good concordance with previous typing methods, like pulse-field gel electrophoresis and RAPD-PCR. (
  • The drawback is that the amount of data generated in the process is large and extracting useful information from it requires new methods to process and analyze it.The main contribution of this thesis is the development of a novel experimental method coined tagRNA-seq, combining 5'tagRACE, a previously developed technique, with RNA-sequencing technology. (
  • Studies based on culture-based and molecular methods have shown an increase in the bacterial community that includes the bacterial phyla Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria in the oropharynx of healthy individuals. (
  • This includes medical and laboratory techniques (such as with bacterial cultures). (
  • The bench-top IR Biotyper system for microbial strain typing is based on Fourier-Transform Infrared (FTIR) spectroscopy technology, and complements Bruker's world-leading MALDI Biotyper mass spectrometry platform for fast microbial identification from cultures using protein fingerprinting. (
  • These tiny droplets were used as microscopic bacterial cultures and reactors. (
  • The proposed method involves using water microdroplets as tiny bacterial cultures and bioreactors and then sorting them based on their fluorescence intensity. (
  • Recently, scientists have questioned if cultures are indeed the reference method for bacterial identification 1,3-4,7 . (
  • Important progress made in the fields of instruments, reagents and techniques have make it easier to adapt to the important changes of the clinical microbiology context e.g. increasing use of microbiology tests, shortage of qualified personnel. (
  • In this prospective study, we sought to characterize the bacterial communities of the respiratory microbiome in healthy children aged between 3 and 6 years old by combining both cultural techniques and sequencing of the 16S rRNA gene. (
  • nov. is proposed, with 200901116(T) ( = CIP 110703(T) = DSM 28999(T)) as the type strain. (
  • This makes the IR Biotyper an efficient tool for fast and cost-effective strain typing. (
  • Due to its fast time-to-result (TTR), excellent strain differentiation performance, low cost per sample, ease of use, robustness and throughput, the IR Biotyper can perfectly complement next-generation sequencing (NGS) strain typing, which typically requires more time, training and infrastructure in core labs. (
  • Bismuth and cefiderocol showed synergistic effect both in vitro and in vivo (lung infectious model) against PAO1, a strain of Pseudomonas aeruginosa, as a metallo-sideromycin complex transported actively into bacterial cells. (
  • The type strain S. saprophyticus ATCC 15305 and the already characterized clinical isolate 7108 were used as controls. (
  • the type strain is 656.84 T (5DSM 23593 T 5CIP 101062 T). (
  • To this end, the researchers first used a type of biosensor called Q-body to measure the amount of the desired protein produced by each strain. (
  • Thus, fluorescence intensity is directly related to the amount of the desired protein that each bacterial strain can produce. (
  • This incorporation enables the active transport of the antibiotic into bacterial cell through nutrient pathways. (
  • An SBI was defined as isolation of a bacterial pathogen from urine, blood, cerebrospinal fluid or stools, an IBI as isolation of a bacterial pathogen from blood or cerebrospinal fluid. (
  • Real-time polymerase chain reaction (PCR) is a laboratory technique for pathogen detection. (
  • Her research has involved using molecular, cell and computational biology tools and techniques to understand the pathological mechanisms underlying neurological disorders. (
  • However, the function of this protein has remained unclear because pilU mutants exhibit inconsistent phenotypes among type IV pilus systems and because it is relatively understudied compared to PilT. (
  • Type IV pili are composed almost exclusively of a single protein called the major pilin, which forms a helical fiber that extends from the cell surface [ 6 ]. (
  • 1-dimensional and 2-dimensional electrophoretic techniques provide powerful tools for the analysis of complex protein mixtures. (
  • There are two types of blood pressure: primary hypertension and secondary hypertension. (
  • Fortunately, it's not as severe as other types of pneumonia, and its symptoms are easily treatable. (
  • With acute bacterial prostatitis, symptoms tend to be more severe. (
  • The time taken to make the identification is currently less than a day, however with further development and streamlining of the technique this will be further reduced. (
  • For the fungal type (eumycetoma), treatment is based on administration of antifungal agents for prolonged periods, usually preceded or followed by surgical excision of the lesions. (
  • This sampling technique is mainly used for fluid-containing lesions. (
  • RÉSUMÉ La présente étude visait à caractériser des isolats de P. aeruginosa dans deux unités de soins intensifs en Arabie saoudite et en Égypte. (
  • We paired these experiments with genome sequencing techniques to identify changes to the bacterial genetic code. (
  • Here, we study the type IVa competence pilus of Vibrio cholerae as a model system to define the role of PilU. (
  • This problem could be significantly mitigated by coordinately screening multiple tissue types simultaneously in a high-throughput fashion during upstream tier 1 screening. (
  • When Y. pestis attacks a cell it uses the type-III pathway--a needle-like projection--to inject various toxins into the cell, killing it. (
  • Mycetoma has two distinct etiologies: fungal and bacterial. (
  • as well as diagnostic techniques, drug development and tissue transplantation. (
  • In children, primitive neuroectodermal tumors are the most common cause of leptomeningeal carcinomatosis, but pilocytic astrocytomas (which are generally low grade) occasionally can demonstrate this type of aggressive behavior. (
  • Each laboratory was randomly allocated 14 filters per dust type. (
  • Computer systems of 2-dimensional gel analysis namely PDQUEST and BioImage were implemented and databases were established for the studied cell types. (
  • IHC, in combination with microscopy and image analysis techniques, has become a very powerful tool that offers a direct visualization of tissue antigens using labeled antibodies specific to the antigen. (
  • Schematic for SESI-MS analysis of bacterial volatiles. (
  • After developing a concentration method, analysis of Adenosine TriPhosphate (ATP) was a promising compliance technique following tests in UV-treated seawater. (
  • Another technique brought up in the presentation to diagnose VAP was the rapid automated microscopy, mini bronchoalveolar lavage (mini-BAL). (
  • Vaccines of this type, prepared from suspensions of inactivated Bordetella pertussis bacterial cells, have been licensed for routine vaccination of infants since the mid-1940s. (
  • Immunohistochemistry (IHC) is an important evaluation tool that falls under the umbrella of immunostaining techniques and exploits antigen - antibody binding to study the status of target molecules in tissues of interest. (
  • The use of the technique of nuclear transfer for reproduction of human beings is surrounded by strong ethical concerns and controversies and is considered a threat to human dignity. (
  • This technique is surrounded by strong ethical concerns and is considered a threat to human dignity. (
  • Work conducted by PhD student Nathaniel Wand has proven that the DNA barcoding technique is able to identify DNA fragments in a mixed sample, containing segments such as those which provide a marker of resistance in MRSA, which would be very beneficial to identify in a healthcare setting. (
  • And so we work to enhance our interrogation techniques, for while extracting information from a biological system is challenging, it is also indispensable to a range of missions: revealing how cells organize into tissues, dissecting disease pathogenesis, and advancing diagnostic and treatment efforts. (
  • In the search for a new, potential drug candidate for type 2 diabetes treatment, these new findings guided Implexion Pharma in their target selection for such a candidate during a research project in 2020-2021. (
  • Filtration followed by chlorination or ultraviolet irradiation are the most common BWMS treatment techniques. (
  • Impact of bacterial and somatic cells content on quality fresh milk in small-scale dairy farms in Kosovo. (
  • Part of the discrepancy may be ascribed to differences in the viability of the starting cells, in eliciting factors, in separation techniques and in storage conditions. (
  • For example, the data in the sheet "DI waterRun_10xDilution" shows pressure reading across a membrane filter when DI water is infused in, while the sheet "BacterialRun_10xDilution" shows the pressure reading across the same filter when the bacterial stock solution is diluted by 10 times and infused in. (
  • The first aim was to evaluate the effects of mixing technique (hand-mixing or auto-mixing) on bacterial attachment to polyether impression materials. (
  • The various techniques can be used for cytological examination, including impression smears, swabs, scrapings, acetate tape preparations and a fine needle aspiration. (
  • Similar to other cell types, neutrophilic granulocytes also release extracellular vesicles (EVs), mainly medium-sized microvesicles/microparticles. (
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (
  • By labelling the bacterial DNA at specific points and then imaging a sample of the DNA spread on a surface so that the DNA strands are extended, it is possible to read a 'DNA barcode', where the positions of the label reveal the DNA sequence, in the same way as the bars in a barcode provide an identifying pattern. (
  • There is a clear need for techniques that are able to provide the same information in a shorter time period. (
  • Additionally, disposable toothbrushes can provide hygienic benefits by reducing the risk of bacterial contamination and cross-contamination, as they are intended for short-term use and then discarded. (
  • The drug discovery company Implexion Pharma and researchers from Lund University have explored new potential drug candidates for type 2 diabetes using X-ray crystallography research techniques at MAX IV. (
  • A recent finding has indicated that the amino acid-derived metabolite imidazole propionate (ImP) in patients with type 2 diabetes could contribute to impaired glucose tolerance and reduced insulin signalling. (
  • High blood pressure is often the first symptom to precede potentially fatal health conditions like type 2 diabetes, heart attack, and stroke. (
  • Bacterial type IV pili are critical for diverse biological processes including horizontal gene transfer, surface sensing, biofilm formation, adherence, motility, and virulence. (
  • The tissue samples are prepared following specialized techniques to enable the entry of the antibodies, and the label is detected using light or electron microscopy (Figure 2). (
  • This technique is most often used for samples taken from draining tracts, ear canals, or interdigital webs. (
  • Enterococci were isolated from infected patients by classical microbiologic techniques (3) . (
  • The largest manifestation of such aseptic techniques is in hospital operating theaters, where the aim is to keep patients free from hospital micro-organisms. (
  • En Égypte, des isolats prélevés sur les tubulures d'appareils d'aspiration et de respiration artificielle (A8) et sur les cuvettes (A7) étaient liés à ceux prélevés chez les patients ayant ces antibiotypes. (
  • Small intestinal bacterial overgrowth (SIBO) appears to be common in patients with Crohn's disease (CD). (