Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Cell Adhesion: Adherence of cells to surfaces or to other cells.Adhesiveness: A property of the surface of an object that makes it stick to another surface.Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Cell Adhesion Molecules: Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Silicone Elastomers: Polymers of silicone that are formed by crosslinking and treatment with amorphous silica to increase strength. They have properties similar to vulcanized natural rubber, in that they stretch under tension, retract rapidly, and fully recover to their original dimensions upon release. They are used in the encapsulation of surgical membranes and implants.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Staphylococcus epidermidis: A species of STAPHYLOCOCCUS that is a spherical, non-motile, gram-positive, chemoorganotrophic, facultative anaerobe. Mainly found on the skin and mucous membrane of warm-blooded animals, it can be primary pathogen or secondary invader.Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Tissue Adhesions: Pathological processes consisting of the union of the opposing surfaces of a wound.Intercellular Adhesion Molecule-1: A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.Fimbriae Proteins: Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).Focal Adhesions: An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS.Hexanols: Isomeric forms and derivatives of hexanol (C6H11OH).Mannosides: Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Polyvinyl Chloride: A polyvinyl resin used extensively in the manufacture of plastics, including medical devices, tubing, and other packaging. It is also used as a rubber substitute.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Silicones: A broad family of synthetic organosiloxane polymers containing a repeating silicon-oxygen backbone with organic side groups attached via carbon-silicon bonds. Depending on their structure, they are classified as liquids, gels, and elastomers. (From Merck Index, 12th ed)Streptococcus mitis: A species of gram-positive, coccoid bacteria commensal in the respiratory tract.Microscopy, Atomic Force: A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.Vascular Cell Adhesion Molecule-1: Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)Coated Materials, Biocompatible: Biocompatible materials usually used in dental and bone implants that enhance biologic fixation, thereby increasing the bond strength between the coated material and bone, and minimize possible biological effects that may result from the implant itself.Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Hemagglutination: The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).Streptococcus sanguis: A gram-positive organism found in dental plaque, in blood, on heart valves in subacute endocarditis, and infrequently in saliva and throat specimens. L-forms are associated with recurrent aphthous stomatitis.Polystyrenes: Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads.Plastics: Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Titanium: A dark-gray, metallic element of widespread distribution but occurring in small amounts; atomic number, 22; atomic weight, 47.90; symbol, Ti; specific gravity, 4.5; used for fixation of fractures. (Dorland, 28th ed)Prostheses and Implants: Artificial substitutes for body parts, and materials inserted into tissue for functional, cosmetic, or therapeutic purposes. Prostheses can be functional, as in the case of artificial arms and legs, or cosmetic, as in the case of an artificial eye. Implants, all surgically inserted or grafted into the body, tend to be used therapeutically. IMPLANTS, EXPERIMENTAL is available for those used experimentally.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Focal Adhesion Kinase 1: A non-receptor protein tyrosine kinase that is localized to FOCAL ADHESIONS and is a central component of integrin-mediated SIGNAL TRANSDUCTION PATHWAYS. Focal adhesion kinase 1 interacts with PAXILLIN and undergoes PHOSPHORYLATION in response to adhesion of cell surface integrins to the EXTRACELLULAR MATRIX. Phosphorylated p125FAK protein binds to a variety of SH2 DOMAIN and SH3 DOMAIN containing proteins and helps regulate CELL ADHESION and CELL MIGRATION.Bacterial Physiological Phenomena: Physiological processes and properties of BACTERIA.Focal Adhesion Protein-Tyrosine Kinases: A family of non-receptor, PROLINE-rich protein-tyrosine kinases.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Fibronectins: Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Bacterial Proteins: Proteins found in any species of bacterium.Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Materials Testing: The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Serratia marcescens: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Caco-2 Cells: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.Integrins: A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.Hydrophobic and Hydrophilic Interactions: The thermodynamic interaction between a substance and WATER.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Host-Pathogen Interactions: The interactions between a host and a pathogen, usually resulting in disease.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.Neural Cell Adhesion Molecules: Cell adhesion molecule involved in a diverse range of contact-mediated interactions among neurons, astrocytes, oligodendrocytes, and myotubes. It is widely but transiently expressed in many tissues early in embryogenesis. Four main isoforms exist, including CD56; (ANTIGENS, CD56); but there are many other variants resulting from alternative splicing and post-translational modifications. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, pp115-119)Streptococcus mutans: A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Mannose: A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.PolysaccharidesProtein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell Adhesion Molecules, Neuronal: Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.E-Selectin: Cell adhesion molecule and CD antigen that mediates neutrophil, monocyte, and memory T-cell adhesion to cytokine-activated endothelial cells. E-selectin recognizes sialylated carbohydrate groups related to the Lewis X or Lewis A family.Paxillin: Paxillin is a signal transducing adaptor protein that localizes to FOCAL ADHESIONS via its four LIM domains. It undergoes PHOSPHORYLATION in response to integrin-mediated CELL ADHESION, and interacts with a variety of proteins including VINCULIN; FOCAL ADHESION KINASE; PROTO-ONCOGENE PROTEIN PP60(C-SRC); and PROTO-ONCOGENE PROTEIN C-CRK.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Staphylococcal Infections: Infections with bacteria of the genus STAPHYLOCOCCUS.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Cadherins: Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.Bacteriological Techniques: Techniques used in studying bacteria.Antigens, CD18: Cell-surface glycoprotein beta-chains that are non-covalently linked to specific alpha-chains of the CD11 family of leukocyte-adhesion molecules (RECEPTORS, LEUKOCYTE-ADHESION). A defect in the gene encoding CD18 causes LEUKOCYTE-ADHESION DEFICIENCY SYNDROME.Platelet Adhesiveness: The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Antigens, CD29: Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Lymphocyte Function-Associated Antigen-1: An integrin heterodimer widely expressed on cells of hematopoietic origin. CD11A ANTIGEN comprises the alpha chain and the CD18 antigen (ANTIGENS, CD18) the beta chain. Lymphocyte function-associated antigen-1 is a major receptor of T-CELLS; B-CELLS; and GRANULOCYTES. It mediates the leukocyte adhesion reactions underlying cytolytic conjugate formation, helper T-cell interactions, and antibody-dependent killing by NATURAL KILLER CELLS and granulocytes. Intracellular adhesion molecule-1 has been defined as a ligand for lymphocyte function-associated antigen-1.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Vinculin: A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.Genes, Bacterial: The functional hereditary units of BACTERIA.Integrin alpha4beta1: Integrin alpha4beta1 is a FIBRONECTIN and VCAM-1 receptor present on LYMPHOCYTES; MONOCYTES; EOSINOPHILS; NK CELLS and thymocytes. It is involved in both cell-cell and cell- EXTRACELLULAR MATRIX adhesion and plays a role in INFLAMMATION, hematopoietic cell homing and immune function, and has been implicated in skeletal MYOGENESIS; NEURAL CREST migration and proliferation, lymphocyte maturation and morphogenesis of the PLACENTA and HEART.P-Selectin: Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.Cell Aggregation: The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.Neural Cell Adhesion Molecule L1: A member of the immunoglobulin superfamily of neuronal cell adhesion molecules that is required for proper nervous system development. Neural cell adhesion molecule L1 consists of six Ig domains, five fibronectin domains, a transmembrane region and an intracellular domain. Two splicing variants are known: a neuronal form that contains a four-amino acid RSLE sequence in the cytoplasmic domain, and a non-neuronal form that lacks the RSLE sequence. Mutations in the L1 gene result in L1 disease. Neural cell adhesion molecule L1 is predominantly expressed during development in neurons and Schwann cells; involved in cell adhesion, neuronal migration, axonal growth and pathfinding, and myelination.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Focal Adhesion Kinase 2: A non-receptor protein-tyrosine kinase that is expressed primarily in the BRAIN; OSTEOBLASTS; and LYMPHOID CELLS. In the CENTRAL NERVOUS SYSTEM focal adhesion kinase 2 modulates ION CHANNEL function and MITOGEN-ACTIVATED PROTEIN KINASES activity.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Cytoskeletal Proteins: Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Peritoneal Diseases: Pathological processes involving the PERITONEUM.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Cell-Matrix Junctions: Specialized areas at the CELL MEMBRANE where a cell attaches to the EXTRACELLULAR MATRIX or other substratum.Junctional Adhesion Molecules: A family of membrane glycoproteins localized to TIGHT JUNCTIONS that contain two extracellular Ig-like domains, a single transmembrane segment, and a cytoplasmic tail of variable length.Receptors, Lymphocyte Homing: Cell surface glycoproteins on lymphocytes and other leukocytes that mediate adhesion to specialized blood vessels called high endothelial venules. Several different classes of lymphocyte homing receptors have been identified, and they appear to target different surface molecules (addressins) on high endothelial venules in different tissues. The adhesion plays a crucial role in the trafficking of lymphocytes.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Laminin: Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.Umbilical Veins: Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.

Role of antibodies against Bordetella pertussis virulence factors in adherence of Bordetella pertussis and Bordetella parapertussis to human bronchial epithelial cells. (1/5458)

Immunization with whole-cell pertussis vaccines (WCV) containing heat-killed Bordetella pertussis cells and with acellular vaccines containing genetically or chemically detoxified pertussis toxin (PT) in combination with filamentous hemagglutinin (FHA), pertactin (Prn), or fimbriae confers protection in humans and animals against B. pertussis infection. In an earlier study we demonstrated that FHA is involved in the adherence of these bacteria to human bronchial epithelial cells. In the present study we investigated whether mouse antibodies directed against B. pertussis FHA, PTg, Prn, and fimbriae, or against two other surface molecules, lipopolysaccharide (LPS) and the 40-kDa outer membrane porin protein (OMP), that are not involved in bacterial adherence, were able to block adherence of B. pertussis and B. parapertussis to human bronchial epithelial cells. All antibodies studied inhibited the adherence of B. pertussis to these epithelial cells and were equally effective in this respect. Only antibodies against LPS and 40-kDa OMP affected the adherence of B. parapertussis to epithelial cells. We conclude that antibodies which recognize surface structures on B. pertussis or on B. parapertussis can inhibit adherence of the bacteria to bronchial epithelial cells, irrespective whether these structures play a role in adherence of the bacteria to these cells.  (+info)

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (2/5458)

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.  (+info)

Enhanced adhesion of Pasteurella multocida to cultured turkey peripheral blood monocytes. (3/5458)

Capsular hyaluronic acid (HA) mediates adhesion of serogroup A strains of Pasteurella multocida to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. Following culture of TPBM for 6 days in chamber slides, adhesion of the bacteria to TPBM increased gradually. Incubation in chamber slides coated with entactin-collagen IV-laminin (ECL) attachment matrix or exposure to phorbol myristate acetate (PMA) further enhanced the adhesion of P. multocida to TPBM. Addition of HA, but not Arg-Gly-Asp peptide, to TPBM culture inhibited bacterial adherence similarly to the inhibition previously reported for TASM. Exposure of TPBM to monoclonal antibody directed against HA-binding cell surface proteoglycan (CD44) decreased binding of P. multocida. Collectively, these findings indicate that P. multocida adhesion to TPBM is mediated by capsular HA and can be increased by culture on ECL attachment matrix or PMA exposure. Additionally, the findings suggest that the capsular mucopolysaccharide of serogroup A strains of P. multocida recognizes an isoform of CD44 expressed on cultured TPBM.  (+info)

Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. (4/5458)

The Vibrio cholerae genome contains a 5.4-kb pil gene cluster that resembles the Aeromonas hydrophila tap gene cluster and other type IV-A pilus assembly operons. The region consists of five complete open reading frames designated pilABCD and yacE, based on the nomenclature of related genes from Pseudomonas aeruginosa and Escherichia coli K-12. This cluster is present in both classical and El Tor biotypes, and the pilA and pilD genes are 100% conserved. The pilA gene encodes a putative type IV pilus subunit. However, deletion of pilA had no effect on either colonization of infant mice or adherence to HEp-2 cells, demonstrating that pilA does not encode the primary subunit of a pilus essential for these processes. The pilD gene product is similar to other type IV prepilin peptidases, proteins that process type IV signal sequences. Mutational analysis of the pilD gene showed that pilD is essential for secretion of cholera toxin and hemagglutinin-protease, mannose-sensitive hemagglutination (MSHA), production of toxin-coregulated pili, and colonization of infant mice. Defects in these functions are likely due to the lack of processing of N termini of four Eps secretion proteins, four proteins of the MSHA cluster, and TcpB, all of which contain type IV-A leader sequences. Some pilD mutants also showed reduced adherence to HEp-2 cells, but this defect could not be complemented in trans, indicating that the defect may not be directly due to a loss of pilD. Taken together, these data demonstrate the effectiveness of the V. cholerae genome project for rapid identification and characterization of potential virulence factors.  (+info)

Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. (5/5458)

Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae.  (+info)

P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy. (6/5458)

Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose-resistant adherence to HT-29 cells, and expressed P fimbriae (P = 0.0036) as well as other mannose-resistant adhesins (P = 0.012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P = 0.0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants.  (+info)

Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. (7/5458)

Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important human pathogen. The production of several virulence factors by P. aeruginosa is controlled through two quorum-sensing systems, las and rhl. We have obtained evidence that both the las and rhl quorum-sensing systems are also required for type 4 pilus-dependent twitching motility and infection by the pilus-specific phage D3112cts. Mutants which lack the ability to synthesize PAI-1, PAI-2, or both autoinducers were significantly or greatly impaired in twitching motility and in susceptibility to D3112cts. Twitching motility and phage susceptibility in the autoinducer-deficient mutants were partially restored by exposure to exogenous PAI-1 and PAI-2. Both twitching motility and infection by pilus-specific phage are believed to be dependent on the extension and retraction of polar type 4 pili. Western blot analysis of whole-cell lysates and enzyme-linked immunosorbent assays of intact cells were used to measure the amounts of pilin on the cell surfaces of las and rhl mutants relative to that of the wild type. It appears that PAI-2 plays a crucial role in twitching motility and phage infection by affecting the export and assembly of surface type 4 pili. The ability of P. aeruginosa cells to adhere to human bronchial epithelial cells was also found to be dependent on the rhl quorum-sensing system. Microscopic analysis of twitching motility indicated that mutants which were unable to synthesize PAI-1 were defective in the maintenance of cellular monolayers and migrating packs of cells. Thus, PAI-1 appears to have an essential role in maintaining cell-cell spacing and associations required for effective twitching motility.  (+info)

Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. (8/5458)

The influence of pH on the adhesion of two Lactobacillus strains to Caco-2 human intestinal cells was investigated. One strain, Lactobacillus johnsonii La1, was adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same experimental conditions. On the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. Mice were immunized with live La1, and the hybridomas produced by fusion of spleen cells with ONS1 cells were screened for the production of antibodies specific for L. johnsonii La1. A set of these monoclonal antibodies was directed against a nonproteinaceous component of the L. johnsonii La1 surface. It was identified as lipoteichoic acid (LTA). This molecule was isolated, chemically characterized, and tested in adhesion experiments in the same system. The adhesion of L. johnsonii La1 to Caco-2 cells was inhibited in a concentration-dependent way by purified LTA as well as by L. johnsonii La1 culture supernatant that contained LTA. These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.  (+info)

Objective: Relevant animal models to study effects of bacterial aggregates on wound healing are lacking. We aimed at establishing an equine wound model with bacterial aggregates to investigate the impact of bacterial inoculation on normal (thorax) and impaired (limb) wound healing. Approach: Wounds were created on three limbs and both thorax sides of six horses. Twelve out of 20 wounds per horse were inoculated with 104 Staphylococcus aureus and 105 Pseudomonas aeruginosa on day 4. Healing was monitored until day 27 by clinical assessment, including wound scoring, surface pH measurements, and digital photography for area determination. Biopsies were used for bacterial culture and for peptide nucleic acid fluorescence in situ hybridization to detect bacterial aggregates. Results: Inoculated limb wounds healed slower than noninoculated limb wounds from day 10 onward ( p , 0.0001). Inoculated and noninoculated thorax wounds healed equally well and faster than limb wounds. The odds ratio of ...
Purpose: Bacterial adhesion and colonization play a crucial function in the pathogenesis of peri-implant tissue infection, which is considered the main cause of fixture loss. The aim of this study is to evaluate the differences in bacterial adhesion between a machined titanium surface, a double acid etched surface (Osseotite®) and an Osseotite surface with Nanometer-scale Discrete Crystalline Deposition (DCD™) of calcium phosphate (CaP)(Nanotite®).Methods: Surface roughness properties of each sample were determined by a laser profilometer and scanning electron microscopy (SEM) observation. Bacterial adhesion on machined, Osseotite®, and Nanotite® discs were performed using the following bacterial strains: Streptococcus mutans CCUG 35176, Streptococcus sanguis CCUG 17826, Streptococcus salivarius CCUG 11878, Actinobacillus actinomycetecomitans CCUG 37002, Porphyromonas gingivalis CCUG 2521. The assessment of bacterial adhesion was performed by comparing two methods: Total Viable Count (TVC) ...
Many bacteria, both environmental and pathogenic, exhibit the property of autoaggregation. In autoaggregation (sometimes also called autoagglutination or flocculation), bacteria of the same type form multicellular clumps that eventually settle at the bottom of culture tubes. Autoaggregation is generally mediated by self-recognising surface structures, such as proteins and exopolysaccharides, which we term collectively as autoagglutinins. Although a widespread phenomenon, in most cases the function of autoaggregation is poorly understood, though there is evidence to show that aggregating bacteria are protected from environmental stresses or host responses. Autoaggregation is also often among the first steps in forming biofilms. Here, we review the current knowledge on autoaggregation, the role of autoaggregation in biofilm formation and pathogenesis, and molecular mechanisms leading to aggregation using specific examples ...
The adhesin involved in diffuse adherence (AIDA) is an autotransporter protein that confers the diffuse adherence phenotype to certain diarrheagenic Escherichia coli strains. It consists of a 49 amino acid signal peptide, a 797 amino acid passenger domain, and a 440 amino acid beta-domain integrated into the outer membrane. The beta-domain consists of two parts: the beta(1)-domain, which is predicted to form two beta-strands on the bacterial cell surface, and the beta(2)-domain, which constitutes the transmembrane domain. We have previously shown that the beta-domain can be folded from the urea-denatured state when bound to a nickel column during purification. It has not been possible to achieve proper refolding of the beta-domain in solution; instead, a misfolded state C is formed. Here, we characterize this misfolded state in greater detail, showing that despite being misfolded, C can be analyzed as a conventional conformational state, with cooperative unfolding in urea and SDS as well as ...
Structural studies of biological macromolecular assemblies are providing an understanding of cellular function. In our laboratory, we utilize electron microscopy and image reconstruction to investigate questions about how adhesion pili aid pathogenic bacterial survival under harsh physiological conditions. Work in the lab currently focuses on bacterial adhesion pili and poliovirus polymerase. These projects address basic medical research questions directed at understanding bacterial adhesion to human host tissue and viral replication. Our data support development of novel therapeutics targeting these important health issues including, for example, development of a vaccine against travelers diarrhea ...
PhD Project - Molecular mechanisms modulating host epithelial integrity in response to bacterial adhesion at University of Birmingham, listed on FindAPhD.com
Working with a pathogenic strain of E. coli, the researchers found that the bacteria can sense attachment to the human intestinal cells and activate gene expression in response. This was demonstrated by engineering one of these genes to express a protein that stains the expressing bacteria to appear green under the microscope. Under microscopic examination, the researchers observed that only the attached bacteria fluoresce in bright green, whereas non-attached bacteria remain dark ...
Bacterial resistance to antibiotics is increasing at a high rate in both developing and developed countries. To circumvent the problem of drug-resistant bacterial pathogens, we need to develop new effective methods, substances, and materials that can disarm and prevent them from causing infections. However, to do this we first need to find new possible targets in bacteria to approach and novel strategies to apply.Escherichia coli (E. coli) bacteria is a normal member of the intestinal microflora of humans and mammals, but frequently cause diverse intestinal and external diseases by means of virulence factors, which leads to hundreds of million sick people each year with a high mortality rate. An E. coli bacterial infection starts with adhesion to a host cell using cell surface expressed adhesion polymers, called adhesion pili. Depending on the local environment different types of pili are expressed by the bacteria. For example, bacteria found in the gastrointestinal tract commonly express ...
Mannose, Apoptosis, Bacteria, Bacterial Adhesion, Bladder, Cell, Cells, Clathrin, Colitis, Contract, Crohns Disease, Cytoplasm, Diabetes Mellitus, Disease, E Coli, Endoplasmic Reticulum, Epithelial Cell, Epithelial Cells, Escherichia, Escherichia Coli
Fang B, Gon S, Park M, Kumar K-N, Rotello VM, Nusslein K, Santore MM. 2011. Bacterial adhesion on hybrid cationic nanoparticle-polymer brush surfaces: ionic strength tunes capture from monovalent to multivalent binding.. Colloids Surf B Biointerfaces. 87(1):109-15. ...
Biofilms are ubiquitous in aquatic environments. Biofilms have been shown to attract and harbor pathogens such as P. aeruginosa and Legionella pneumophila in premise plumbing system. The fact that biofilms can protect attached bacterial cells from disinfectants raises rudimentary questions regarding interactions of bacterial cells with biofilm surfaces. Consequently, the main objectives of this study were to: 1) investigate the mechanisms that govern E. coli S17, E. coli 14f and Legionella cells adhesion on clean PVC, copper and biofilms; 2) examine the role of disinfectants on biofilms structure and subsequent effect on bacterial adhesion. Mechanisms of three strains of bacteria attachment on biofilms grown on PVC and copper surfaces were investigated. Biofilms were grown in CDC reactors using different types of feed water such as groundwater, monochloramine-treated groundwater, dechlorinated tap water and tap water. Biofilm physical structure was characterized at micro- and meso-scales using ...
The global issue of nosocomial infection is owing to bacterial colonization and biofilm formation on medical devices which primarily affects critically ill and/or immuno-compromised patients and also leads to malfunctioning of the devices. Therefore, it is desirable to prevent bacterial colonization on these devices by coating with a non toxic antimicrobial agent or bacterial adherence inhibitor. Here we have shown Bacillus licheniformis JS2 derived selenium nanoparticles (SeNPs) inhibit Staphylococcus aureus adherence and micro-colony formation on polystyrene, glass, and catheter surface. Results indicated that, the coating of these non toxic biogenic SeNPs, at a concentration of 0.5mgSe/ml, prohibits bacterial load to more than 60% on glass and catheter surface, when incubated at 4°C for 24h in phosphate buffered saline. Furthermore, confocal and electron microscopic observations strongly suggested the inhibition of biofilm and micro-colony formation on SeNP coated glass and catheter surfaces ...
Adherence ability of Staphylococcus epidermidis on prosthetic biomaterials: an in vitro study [Corrigendum] Â Shida T, Koseki H, Yoda I, et al. Int J Nanomed. 2013;8(1):3955â 3961.On page 3955, please note that the first affiliation has changed from Department of Orthopedic Surgery, Graduate School of Medicine, Nagasaki University, Nagasaki, Japan to Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.Read the original article
Ileal lesions in Crohns disease (CD) patients are colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to adhere to and invade intestinal epithelial cells (IEC), and to survive within macrophages. The interaction of AIEC with IEC depends on bacterial factors mainly type 1 pili, flagella, and outer membrane proteins. In humans, proteases can act as host defence mechanisms to counteract bacterial colonization. The protease meprin, composed of multimeric complexes of the two subunits alpha and beta, is abundantly expressed in IECs. Decreased levels of this protease correlate with the severity of the inflammation in patients with inflammatory bowel disease. The aim of the present study was to analyze the ability of meprin to modulate the interaction of AIEC with IECs. In patients with ileal CD we observed decreased levels of meprins, in particular that of meprin β. Dose-dependent inhibition of the abilities of AIEC strain LF82 to adhere to and invade intestinal epithelial T84 cells was
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Pappelbaum, Karin I.; Gorzelanny, Christian; Graessle, Sandra; Suckau, Jan; Laschke, Matthias W.; Bischoff, Markus; Bauer, Corinne; Schorpp-Kistner, Marina; Weidenmaier, Christopher; Schneppenheim, Reinhard; Obser, Tobias; Sinha, Bhanu; Schneider, Stefan W. ...
Bacterial biofilms are sessile microbial communities that cause serious problems, such as antibiotic resistant chronic infections in humans, and persistent biofouling of engineering facilities. Biofilm formation is initiated by bacterial adhesion to a surface followed by the formation of microcolonies and further development of heterogeneous structures with water channels between cell clusters. The mechanism of biofilm structural heterogeneity and the bacterial genes involve in structural organization are still poorly understood. Nevertheless, once microbes adhere to a surface and form biofilm on it, they are up to 10-1,000 times more resistant to antimicrobial agents than their free-swimming counterparts. It is well accepted that biofilm formation involves multicellular behaviors, associated with major changes in microbial gene expression and protein synthesis. These changes are influenced by many environmental factors such as surface hydrophobicity, topography, chemistry, and charge. To better
Structural studies of biological macromolecular assemblies are providing an understanding of cellular function. In our laboratory, we utilize electron microscopy and image reconstruction to investigate questions about microbial virulence.. Work in the lab currently focuses on bacterial adhesion pili (fimbriae), type III secretion system needles, and virus replication. These projects address basic medical research questions directed at understanding bacterial adhesion to human host tissue and viral replication. Our data support development of novel therapeutics targeting these important health issues including, for example, development of a vaccine against travelers diarrhea.. ...
In this paired case-control study of infants with diarrhea in São Paulo, we examined the association between HEp-2-adherent Escherichia coli strains and diarrhea. We tested isolates from stool specimens of infants with diarrhea and matched controls in an HEp-2 cell adherence assay; we then hybridized isolates with DNA probes and identified enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), and diffusely adherent E. coli (DAEC). From 100 patient-control pairs, we isolated 78 HEp-2-adherent strains; of these, 61 strains were single pathogens identified in stools of infants with diarrhea. While typical EPEC was significantly associated with diarrhea ( ...
Stinson, Murray W. and Jen Ren Wang (1997) Lectin inhibition of bacterial adhesion to animal cells. [Publication] Full text not available from this repository ...
BioFilm Pharma and BIOASTER (www.bioaster.org), the French Institute for Technological Research in Infectious Diseases and Microbiology, announced today the signature of a research contract.. To address the challenge of antibiotic resistance, this collaboration will lead to the deciphering of the mode of action of a new class of drugs.. This first project aims at identifying the mode of action of non-antibiotic anti-biofilms molecules through an integrated multi-omic analysis.. Anti-biofilm candidates of the study have already shown very promising results thanks to the BioFilm Ring Test® technology patented by the BioFilm Technologies group. As of today, this key technology is the only reliable and standardized in vitro biofilm method focusing on bacterial adhesion. Find more information on BIOASTER Press Release here.. ...
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Much work on bacterial adhesion has focussed on the colonisation of surfaces and the removal of mature biofilms. Little attention has been devoted to interactions within mature biofilms and how these might be manipulated in the cause of novel therapies. Calcium binding to oral streptococci displays …
The mechanisms by which bacteria adhere to inert surfaces are not well understood. The aim of this work was to elucidate the structural and genetic changes induced in a classical E. coli K-12 strain by a mutation allowing surface colonization. Electron microscopy of negatively stained bacteria revealed the presence of thin fibrillar pili at the surfaces of the mutant cells (Fig. 6). These particular pili seemed to be identical to the Congo red-binding structures described by Olsén and coworkers (22) as curli. When grown on CFA-Congo red indicator plates, the mutant strains were more intensely stained, indicating curli overproduction. The curli subunits are encoded by the csgA gene (21). Insertion of a reporter cassette into this gene revealed a 3.5-fold-higher transcription in the presence of the mutation responsible for the adherent phenotype. Furthermore, the introduction of a csgAnull mutation totally suppressed the biofilm-forming properties (Fig.2), demonstrating that curli production is ...
casSAR Dugability of Q2FUY2 | clfB | Clumping factor B - Also known as CLFB_STAA8, clfB. Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Partly responsible for mediating bacterial attachment to the highly keratinized squamous epithelial cells from the nasal cavity via an interaction with cytokeratin K10 (K10). Also promotes bacterial attachment to cultured keratinocytes, possibly through an interaction with cytokeratin K10. Binds mouse cytokeratin K10. Activates human platelet aggregation.
Microtiter plate-based bacterial biofilm assay is frequently used to study bacterial biofilm development and growth. While this assay is simple and relatively high-throughput, it frequently shows difficulty in establishing robust biofilm attachment in the wells. We report that the consistency of bacterial biofilm a
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es found that the beads and the bacterial aggregates collided up to a ... The Penn State engineer explains that the fact that large aggregates...Now using the new approach developed by Logan researchers not only ...In addition in studying the ocean processes Logan has identified a ......,Faster,coagulation,rates,found,in,natural,systems,could,impact,industrial,processing,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
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Spi-Ceramic coatings are highly adherent nanocrystalline thin films deposited using N2 Biomedicals low temperature ion beam assisted deposition process. Al
Methods for treating patients in which damaged tissue or an indwelling prosthetic device or catheter has a bacterial biofilm growing thereon, to at least partially disrupt said biofilm, by administering at least one antibacterial enzyme that is lethal or damaging to the biofilm-forming bacteria in an amount that is effective to at least partially disrupt the biofilm upon contact therewith. Methods for prophylactically treating a patient, and methods for disinfecting or sterilizing a surface ex-vivo to remove a biofilm or prevent biofilm growth are also disclosed, as well as implantable articles susceptible to biofilm growth to which a prophylactic coating of an antibacterial enzyme has been applied.
How to treat HEK293 with S9 fraction? - posted in Tissue and Cell Culture: Hi, everyone In my experiment, i need to treat HEK293 cells with drug including S9 fraction. In the protocol of S9. The medium including S9 have to remove and wash with PBS after treating in 4 hours. But the adhesion ability of HEK293 cells are very week. The cells will be lost if i wash them. What can i do to improve it? Thanks a lot.
W]hile many behavioral traits have a heritable component, its not anything like what the naive extremists among the cognitive science crowd think. There are no genes that specify what you will name your dog [WTF? -ed]- in fact, most of the genes associated with the brain have very wide patterns of expression and functions that are not neatly tied to behaviors: how does an allele of an adhesion factor map to your performance on a math test? It doesnt, not directly. ...
Viral surface protein implicated in the binding to specific host surface molecule(s). This binding can lead to virion entry into the host cell, it can trigger signaling pathways, or it can allow the virion to be carried by the host cell to a specific organ ...
A community-based life style is the normal mode of growth and survival for many bacterial species. These cellular accretions or biofilms are initiated upon reco
whats a Eucaryotic cell?http://library.thinkquest.org/C004535/eukaryotic_cells.html. thanx, you guys are awesome!!. Any time!. eucaryote= no nucleu...
Methicillin resistant Staphylococcus aureus adhesion to human umbilical vein endothelial cells demonstrates wall shear stress dependent ...
The O26 serogroup of enteropathogenic Escherichia coli (EPEC) is one of the serogroups most frequently implicated in infant diarrhea and is also common among enterohemorrhagic E. coli (EHEC) strains. the most common O26 strains belong to EPEC/EHEC serotype O26:H11 and are generally Shiga toxin (Stx) positive. Stx-negative E. coli strains that are negative for the EPEC EAF plasmid and bundle-forming pilus (Bfp) are classified as atypical EPEC. Here, we report a novel adhesin present in an stx-negative bfpA-negative atypical EPEC O26:H11 strain isolated from an infant with diarrhea. A cloned 15-kb genomic region from this strain, designated the locus for diffuse adherence (lda), confers diffuse adherence on HEp-2 cells when expressed in E. coli K-12. Sequence analysis of lda revealed a G+C content of 46.8% and 15 open reading frames sharing homology with the E. coli K88 fae and CS31A clp fimbrial operons. the lda region is part of a putative 26-kb genomic island inserted into the proP gene of the ...
Enteropathogenic Escherichia coli (EPEC) strains possess genes for attaching and effacing (eae) and EPEC adherence factor (EAF) plasmid. It is necessary to develop molecular techniques for the evaluation of EPEC isolates. A total of 183 E. coli isolates from neonates admitted to Pusan National University Hospital were investigated by polymerase chain reaction (PCR) and DNA colony hybridization. Of the 183 isolates tested, 10 (5.5%) were positive for eae by PCR and DNA colony hybridization and confirmed to be EPEC. Ten EPEC isolates showed 3 different adherence patterns: seven strains had diffuse adherence, two localized adherence-like adherence, and one aggregative adherence. They were also examined by antimicrobial susceptibility tests, serotyping, and molecular epidemiological typing such as pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) analysis. The EPEC isolates could be divided into 9 different antimicrobial resistance patterns, 6 serotypes, 4 PFGE ...
Numerous studies have demonstrated the effects of laser-induced heat on demineralization of enamel; however, no studies have investigated the link between heat/laser-induced changes in physicochemical properties and bacterial adhesion. In this study, we investigated the effects of thermal treatment on surface properties of enamel such as hydrophobicity and zeta potential. Bacterial adhesion to treated surfaces was characterized by confocal laser scanning microscopy, and adhesion force was quantified by atomic force microscopy. The hydrophobicity of enamel increased after heating (p < 0.05), and the zeta potential of heated enamel became more negative than that of the control (p < 0.01). Streptococcus oralis and S. mitis were more hydrophilic than S. sanguis, with more negative zeta potential (all p < 0.01). S. mitis and S. oralis occupied significantly less area on enamel after being heated (p < 0.05). Heating reduced the adhesion force of both S. mitis and S. oralis to enamel with ...
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TY - JOUR. T1 - Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry. AU - Mohamed, Nehal. AU - Visai, Livia. AU - Speziale, Pietro. AU - Ross, Julia M.. PY - 2000. Y1 - 2000. N2 - The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the staphylococcal surface using flow cytometry. Our results are in agreement with previous reports on the transcription of the respective genes and demonstrate different patterns of temporal expression for the two adhesins in the strains studied. We demonstrate a convenient technique for quantification of bacterial adhesins that can be used in studies aimed at characterization of bacterial adhesion to ECM components and understanding expression of adhesins during the course of an ...
Definition : Solutions designed to act as a temporary barrier inhibiting postsurgical adhesion between tissues and organs. These solutions are applied to the surface of tissues and organs at the end of surgery, before surgical closure, and are typically resorbed by the body in a short period (e.g., several days). Postsurgical adhesion inhibition solutions are intended for use in pelvic and gynecological surgery, both in open and laparoscopic procedures.. Related Terms : "Films, Postsurgical Adhesion Inhibition" , "Gel, Postsurgical Adhesion Inhibition". Entry Terms : "Surgical Adhesion Barrier Solutions" , "Adhesion Inhibition Solutions" , "Adhesion Barrier Solutions" , "Scarring Inhibitors" , "Postsurgical Scarring Inhibitors" , "Barriers, Adhesion, Resorbable". UMDC code : 20374 ...
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early (,24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P,0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive ...
J. Saunier, J.M. Herry, C. Marliere, M. Renault, M.-N. Bellon-Fontaine, et al.. Modification of the bacterial adhesion of Staphylococcus aureus by antioxidant blooming on polyurethane films. Materials Science & Engineering. C, Materials for Biological Applications, 2015, 56, pp.522-531. 〈hal-01569038〉 ...
In natural environments, bacteria are often found as sessile communities known as biofilms (8, 10). To date, the bacterial structures of adherence (25, 40-42, 56) and the physiological processes involved in bacterial surface colonization (15, 40, 41) are better understood than the genetic responses of bacteria adhering to a surface. By using a library oflacZ fusions and a reliable screen for identifying genes whose expression changes in biofilm versus planktonic cells, the transcription of 38% of the E. coli genes was shown to be modified during the colonization process. Several genes with altered expression in biofilms were identified. Different cellular functions were induced in attached bacteria: the OmpC porin, the high-affinity transport system of glycine betaine, colanic acid production (theE. coli class I exopolysaccharide), tripeptidase T, and synthesis of a nickel high-affinity transport system. On the other hand, the syntheses of flagella and of a putative protein of 92 amino acids ...
Methods of inhibiting bacterial adhesion to medical implants and reducing device-associated infection are effectuated by administering an effective amount of apo-transferrin to an individual with such an implant. Preferably the apo-transferrin is administered by controlled release at or near the implant.
The Scientific World Journal is a peer-reviewed, Open Access journal that publishes original research, reviews, and clinical studies covering a wide range of subjects in science, technology, and medicine. The journal is divided into 81 subject areas.
Streptococcus pneumoniae (pneumococci) are Gram-positive cocci and commensals of the human upper respiratory tract. Pneumococcal pathogenesis requires adherence to host cells and dissemination through cellular barriers and to evade host defense mechanisms. The Pneumococcal surface protein C (PspC) is an important virulence factor which has a crucial role in pneumococcal adhesion to host cells and immune evasion by manipulating the host complement system. To elucidate the pneumococcal adherence and uptake mechanism via factor H glycosaminoglycans (dermatan sulfate and heparin) were employed as competitive inhibitors in infection experiments with epithelial cells or human polymorphonuclear leukocytes (PMNs). Glycosaminoglycans significantly inhibited the FH mediated pneumococcal adherence and subsequent invasion to host epithelial cells. Furthermore, the short consensus repeats of FH which promotes the adhesion of pneumococci to host cells were identified by blocking experiments with domain mapped
Streptococcus pneumoniae (pneumococci) are Gram-positive cocci and commensals of the human upper respiratory tract. Pneumococcal pathogenesis requires adherence to host cells and dissemination through cellular barriers and to evade host defense mechanisms. The Pneumococcal surface protein C (PspC) is an important virulence factor which has a crucial role in pneumococcal adhesion to host cells and immune evasion by manipulating the host complement system. To elucidate the pneumococcal adherence and uptake mechanism via factor H glycosaminoglycans (dermatan sulfate and heparin) were employed as competitive inhibitors in infection experiments with epithelial cells or human polymorphonuclear leukocytes (PMNs). Glycosaminoglycans significantly inhibited the FH mediated pneumococcal adherence and subsequent invasion to host epithelial cells. Furthermore, the short consensus repeats of FH which promotes the adhesion of pneumococci to host cells were identified by blocking experiments with domain mapped
Adherence of M. pneumoniae to a host cell (usually a respiratory tract cell, but occasionally an erythrocyte or urogenital lining cell) is the initiating event for pneumonic disease and related symptoms. The specialized attachment organelle is a polar, electron dense and elongated cell extension that facilitates motility and cytadherence to host cells. It is composed of a central filament surrounded by an intracytoplasmic space, along with a number of adhesins and structural and accessory proteins localized at the tip of the organelle. A variety of proteins are known to contribute to the formation and functionality of the attachment organelle, including the accessory proteins HMW1â "HMW5, P30, P56, and P90 that confer structure and adhesin support, and P1, P30 and P116 which are involved directly in attachment. This network of proteins participates not only in the initiation of attachment organelle formation and adhesion but also in motility. The P1 adhesin (trypsin-sensitive protein) is a 120 ...
Large externalized, repeat-rich proteins are emerging as important factors in the attachment of bacteria to biotic and abiotic surfaces. An intriguing new study of the plant-associated terrestrial microbe Pseudomonas putida by Manuel Espinosa-Urgels group that is reported in this issue of Molecular Microbiology has revealed that LapF, a huge protein (, 6000 aa) associated with the cell surface, is required for microcolony assembly from single attached cells, and in turn, formation of biofilms. Mutants defective in IapF exhibit competitive deficiencies in the rhizosphere. On both biotic and abiotic surfaces, these mutants undergo normal irreversible attachment, but cannot advance beyond this point to form multicellular clusters. The lapF phenotype is nutritionally conditional and is only manifested under a subset of growth regimes. Accordingly, lapF gene expression is controlled by the stress-responsive sigma factor RpoS and is elevated within growing microcolonies on abiotic surfaces and plant ...
Bacterial adhesins promote colonization at the initial stages of an infection by mediating attachment to host tissues, thus avoiding nonspecific host defenses such as mechanical clearance and allowing bacterial multiplication to occur within the host (1). To exert these functions, adhesins need to be presented at the surface of the bacterium. Like typical adhesins, B. pertussis FHA attaches the bacterium to receptors in the respiratory tract (17-21, 28). However, in addition to being surface-associated, large amounts of FHA are also released into the extracellular milieu (15). This has so far only been observed in vitro. In this work, we show for the first time that FHA is likely to also be released in vivo, and that its secretion is necessary for efficient colonization in a mouse model of infection. Our results support the paradigm that the secreted form of bacterial adhesins may participate in pathogenesis.. The use of B. pertussis strains deficient in FHA release but presenting FHA ...
Abstract Expression of the platelet-activating factor receptor is upregulated in the respiratory epithelium of smokers and chronic obstructive pulmonary disease patients. We have recently determined that increased expression of PAFr cor - relates with higher levels of adhesion to human bronchial epithelial cells by non-typable Haemophilus influenzae and Streptococcus pneumoniae which are major bacterial pathogens in acute exacerbations of COPD. In addition, we found that a PAFr antagonist decreased the adhesion of both respiratory bacterial pathogens to non-cigarette exposure con - trol levels. This highlights the possibility that epithelial receptors, that are upregulated in response to cigarette smoke, could be targeted to specifically block chronic bacterial infections of the lower respiratory tract. In this commentary, we explore the question of whether adhesion to a temporally-upregulated host receptor is a common event in chronic bacterial disease, and as such, could represent a putative ...
Biofilm Growth - Pseudomonas aeruginosa. Rocks in a stream bed are often slippery due to the growth of a mixed population of attached bacteria, algae and fungi. These Pseudomonas aeruginosa (bacteria) are shown growing on the surface of a fiber and forming a single species biofilm. - Stock Video Clip K003/2837
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Wounds are known to serve as portals of entry for group A Streptococcus (GAS). Subsequent tissue colonization is mediated by interactions between GAS surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen-like protein-1, Scl1, selectively binds the cellular form of fibronectin (cFn) and also contributes to GAS biofilm formation on abiotic su
Invasin and intimin are major virulence factors of enteropathogenic Yersiniae and Escherichia coli, mediating invasion into and intimate adherence to host cells, respectively. Several studies have hinted that extracellular portion of these homologous proteins might be exported via an autotransport mechanism, but rigorous experimental proof has been lacking. Here, we present a topology model for invasin and intimin, consistent with the hypothesis that the N-terminal β-barrel domain acts as a translocation pore to secrete the C-terminal passenger domain. We confirmed this topology model by inserting epitope tags into the loops of the β-barrel. We further show that obstructing the pore of β-barrel hinders the export of the passenger domain. As for classical autotransport, the biogenesis of invasin and intimin is dependent on the Bam complex and the periplasmic chaperone SurA, whereas the chaperone/protease DegP is involved in quality control. However, compared to classical autotransporters (Type Va
The aim of this in-vitro study was to investigate the influence of fillers in resin composites on the initial bacterial adhesion. For this investigation the strains of S. mutans and S. sanguinis ere used. Additionally, the influence of a pellicle, the inhibitor BHT and silane-treatment of the filler on the bacterial adhesion were examined. The adhesion of the two strains S. mutans and S. sanguinis was also compared. The 13 tested materials were experimental composites, based on the same monomer matrix. One of the composites was without fillers (Grundmasse). The other twelve composites showed a filler-weight fraction of 30%. Two different materials of fillers were used (SiO2, Ba-Al-B-Silikat) with different specific surfaces (0,6 to 150 m2/g). Two materials (K6 0% BHT, K6 1% BHT) differed in silane-treatment of the fillers (silane-treated or not silane-treated). For six composites (Ox 50, R709, DT4, GK 0,7 UF silane-treated, K6 silane-treated, K6 not silane-treated) there was a difference in the ...
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Research groupsCell biology and Biotechnology Mechanisms of gene regulation and bacterial biofilm development Dr Fernando Govantes. ..
Biofilm formation - posted in Microbiology: Hello to forum members, I have 2 questions and your input is greatly appreciated. 1- What is the best way to grow bacterial biofilm on a plate and food? I am doing some reading on that and have not been able yet to tell the best way. Any one worked in biofilm??? Thx in advance!
Encounters with Epithelial Cells Over the past decade, many bacterial pathogens have been shown to enter epithelial cells (Fig. 114-2); the bacteria often use specialized surface structures that bind to receptors, with consequent internalization. However, the exact role and the importance of this process in infection and disease are not well defined for most of these pathogens. Bacterial entry into host epithelial cells is seen as a means for dissemination to adjacent or deeper tissues or as a route to sanctuary to avoid ingestion and killing by professional phagocytes. ...
Define adhesin: any of various specialized molecular components (such as proteins) on the surface of a bacterial cell that… - adhesin in a sentence
NBIC are hosting a third workshop, on Biofilm Management, after the success of previous workshops on Biofilm Detection and Biofilm Engineering, to explore challenges, opportunities and advances in Biofilm Management.
Neves, B. C., Knutton, S., Trabulsi, L. R., Sperandio, V., Kaper, J. B., Dougan, G., & Frankel, G. (1998). Molecular and ultrastrutural characterization of the EspA filaments from different EPEC serotypes. In Abstracts. Atlanta ...
Spontaneous bacterial colonization by CONS in Mgb-/- females versus Mgb+/- and WT controls.Bacteria recovered from urine (CFU/ml), bladders, and kidneys (CFU/or
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Looking for online definition of leukocyte adherence assay test in the Medical Dictionary? leukocyte adherence assay test explanation free. What is leukocyte adherence assay test? Meaning of leukocyte adherence assay test medical term. What does leukocyte adherence assay test mean?
Gregory Anderson, biology department at IUPUI, will present Molecular Insights into Bacterial Biofilm Formation in the Cystic Fibrosis Lung. Read more about Anderson at https://science.iupui.edu/people/anderson-gregory .
Human enterotoxigenic Escherichia coli adhere to the brush border of human enterocytes. The mean number of bacteria adhering to one enterocyte (adhesion index) varied from 0.5 to 3.1 when the strains produce adhesins. Different factors related to enterocytes and to bacteria are involved in this variability. The number of bacteria which adhered to enterocytes issued from the same donor varied from from 0 to 12. Moreover the proportion of enterocytes on which several bacteria sticked did not exceed 20%. This variability might be due to the disparity in the maturation of the enterocytes. On the other hand, whatever the adhesion factors considered, the adhesion index varied according to the donors. ETEC strains did not express adhesion when bacteria were grown in a liquid medium but this capacity could be restored after transfer on solid medium. This phenomenon seemed like a phase-variation and appeared to be linked to a 4 to 6 kilobases (kb) plasmid. On the other hand, when the bacteria were grown on agar
Certain EAEC strains have the potential to cause UTIs.Some of the urine isolates from hospital patients represent IPEC or at least they carry virulence-associated genes of diarrheagenic E. coli. Twenty-eight (10.9%) of the 265 urine isolates carried one or more known IPEC virulence genes, sometimes in combination with classical UPEC virulence genes. From these isolates, 23 carried the EAEC heat-stable enterotoxin 1-coding gene astA, and four of them clearly exhibited the typical EAEC aggregative adherence phenotype on both HEp-2 and T24 bladder epithelial cells. Although the function of this enterotoxin is not completely understood, it has been associated with diarrhea in children (47). Despite the fact that astA and, less frequently, the aap gene have been reported in E. coli pathotypes other than EAEC (39, 40, 48-52), their detection remains useful for the diagnosis of EAEC infection if they are screened in combination with other EAEC genes. However, the widespread presence of the astA gene in ...
Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. The ability of this bacterium to adhere to epithelial cells is considered as an essential early step in colonization and infection. By screening a whole genome phage display library with sera from infected patients, we previously identified three antigenic fragments matching open reading frame spr0075 of the strain R6 genome. This locus encodes for an 120-kDa protein, herein referred to as plasminogen- and fibronectin-binding protein B (PfbB), which displays an LPXTG cell wall anchoring motif and six repetitive domains. In this study, by using isogenic pfbB-deleted mutants of the encapsulated D39 and of the unencapsulated DP1004 type 2 pneumococcal strains, we show that PfbB is involved in S. pneumoniae adherence to various epithelial respiratory tract cell lines. Our data suggest that PfbB directly mediates bacterial adhesion, because fluorescent beads coated with the recombinant PfbB sp17 fragment (encompassing one ...
The authors have described a new model to directly study bacterial adherence to basement membrane using scanning electron microscopy 10. By using direct manual counting of surface adherent P. aeruginosa bacilli with scanning electron microscopy, they have determined the exact number of adherent bacteria on the collagen surface. This could be a more direct and specific, albeit more laborious, method to determine bacteria adherence than previous indirect assays ofbacterial adherence, such as radiolabelling techniques. Byrecent use of this model, the authors have shown that P. aeruginosa adherence to basement membrane collagen is reduced in the presence of low-dose erythromycin, probably partly due to alteration of bacterial morophology 10. These results showed that the lectin PHA-E, but not A. hypogea, significantly inhibited P. aeruginosa adherence to collagen. PHA-E appeared to inhibit P. aeruginosa adherence at 0.01, 0.1 and 1 mg·mL−1, although only the latter two concentrations inhibited ...
Pseudomonas aeruginosa is an extremely successful pathogen able to cause both acute and chronic infections in a range of hosts, utilizing a diverse arsenal of cell-associated and secreted virulence factors. A major cell-associated virulence factor, the Type IV pilus (T4P), is required for epithelial cell adherence and mediates a form of surface translocation termed twitching motility, which is necessary to establish a mature biofilm and actively expand these biofilms. P. aeruginosa twitching motility-mediated biofilm expansion is a coordinated, multicellular behaviour, allowing cells to rapidly colonize surfaces, including implanted medical devices. Although at least 44 proteins are known to be involved in the biogenesis, assembly and regulation of the T4P, with additional regulatory components and pathways implicated, it is unclear how these components and pathways interact to control these processes. In the current study, we used a global genomics-based random-mutagenesis technique, transposon ...
Flagella and flagellum-mediated motility are integral to the virulence of several gastrointestinal bacterial pathogens (10). For L. monocytogenes, no link has been made between flagella and virulence, although the flagella are important for efficient invasion of tissue culture cells (2, 6). In this study, we investigated the mechanism by which flagella influence the ability of L. monocytogenes to invade host cells and the role of flagella in colonizing mice early in infection. Our results clearly indicate that L. monocytogenes flagella do not function as adhesins to enhance bacterial attachment to and invasion of epithelial cells, but rather function as motility devices contributing more to invasion than a mere increase in probability of bacterium-host cell interaction. Moreover, we show that motile bacteria outcompete nonmotile bacteria for initial colonization of the intestinal tract and liver by L. monocytogenes.. Flagella can function as adhesins, independent of motility, to enhance ...
TY - PAT. T1 - Synthetic Disugar Hydrocarbons as Natural Analogs to Control Microbial Behaviors. AU - Wang,Guirong. AU - Luk,Yan-Yeung. PY - 2017/1/19. Y1 - 2017/1/19. N2 - Synthetic disaccharide hydrocarbons (DSHs) that reactive bacterials swarming motility and inhibit bacterial adhesion and biofilm formation. A library of DSHs were tested in several experiment for the impact on various Pseudomonas aeruginosa populations and compared against existing compounds to determine efficacy and utility. Certain DSHs were also to determine the ability to clear bacteria in a mouse pneumonia model.. AB - Synthetic disaccharide hydrocarbons (DSHs) that reactive bacterials swarming motility and inhibit bacterial adhesion and biofilm formation. A library of DSHs were tested in several experiment for the impact on various Pseudomonas aeruginosa populations and compared against existing compounds to determine efficacy and utility. Certain DSHs were also to determine the ability to clear bacteria in a mouse ...
TY - JOUR. T1 - T cell-mediated increased osteoclast formation from peripheral blood as a mechanism for crohns disease-associated bone loss. AU - Oostlander, A.E.. AU - Everts, V.. AU - Schoenmaker, T.. AU - Bravenboer, N.. AU - van Vliet, S.J.. AU - van Bodegraven, A.A.. AU - Lips, P.T.A.M.. AU - Vries, T.J.. PY - 2012. Y1 - 2012. U2 - 10.1002/jcb.23352. DO - 10.1002/jcb.23352. M3 - Article. C2 - 21898548. VL - 113. SP - 260. EP - 268. JO - Journal of Cellular Biochemistry. JF - Journal of Cellular Biochemistry. SN - 0730-2312. IS - 1. ER - ...
Bacteria love to colonize surfaces inside your body, but they have a hard time getting past your rugged, salty skin. Surgeries to implant medical devices often give such bacteria the opportunity needed to gain entry into ...
Diarrhoea in piglets due to Escherichia coli is an important problem in the pig farming industry. Adhesion of these bacteria to the epithelial cells of small intestine is an essential prerequisite for the incidence of diarrhoea. Among the putative candidate genes associated with adhesion pattern, TFRC (transferrin receptor) gene was localized on targeted region of SSC13 and considered as a positional candidate gene. The present investigation was conducted to evaluate the Indian desi pigs in terms of E. coli adhesion pattern (with Indian isolate) and study TFRC expression profile in different adhesion phenotypes. A total of 4 types of adhesion pattern were observed with different frequencies. RT-PCR analysis revealed that TFRC mRNA expression level was different across the differentially adhesive phenotypes. The increased level of TFRC expression indicates its role in immunity against E. coli mediated diarrhoea.
OBJECTIVE: The present study aimed to investigate the effects of the bacterial biofilm formation on the tonsil surface exposed N-acetyl-cysteine (NAC) and
Synthesis of diverse indole libraries on polystyrene resin - Scope and limitations of an organometallic reaction on solid supports
Bacterial adhesion to other bacteria, to eukaryotic cells, and to extracellular matrix proteins is frequently mediated by cell surface-associated polymers (fimbriae) consisting of one or more subunit proteins. We have found that polymerization of curlin to fimbriae-like structures (curli) on the surface of Escherichia coli markedly differs from the prevailing model for fimbrial assembly in that it occurs extracellularly through a self-assembly process depending on a specific nucleator protein. The cell surface-bound nucleator primes the polymerization of curlin secreted by the nucleator-presenting cell or by adjacent cells. The addition of monomers to the growing filament seems to be driven by mass action and guided only by the diffusion gradient between the source of secreted monomer and the surface of monomer condensation.. ...
Fluorescent antinuclear antibody (ANA) testing was performed on 141 sera from 114 patients with well defined rheumatic diseases including fibrositis syndrome and 24 sera from 24 healthy subjects using HEp-2 cells and rat liver as substrates. ANA titers were almost always higher on HEp-2, in most cas …
immune Uncategorized Bosutinib, LDHAL6A antibody Bacterial biofilm has been shown to play a role in delaying wound healing of chronic wounds, a major medical problem that results in significant healthcare burden. gradually cleared from your wounds while the presence of (part of the normal mouse pores and skin flora) improved. Scabs from all unhealed wounds contained 107 study of bacterial biofilm reactions to sponsor defenses and the effects of biofilms on sponsor wound healing pathways. It may also be used to test anti-biofilm strategies the treatment of chronic wounds. spp., and [5C7] have been isolated from chronic wounds, even though the wound may not display any medical indications of localized illness. Multiple bacterial varieties, usually two to five varieties, reside concurrently on a single ulcer [7C9]. The chronicity of unhealed wounds is definitely associated with higher proportion of colonization by anaerobic bacteria and greater variety of aerobic varieties [5]. More recent studies ...
Ideal Bowel Support® LP299v® contains a clinically-documented, human-origin probiotic strain, L. plantarum 299v, that resists stomach acid and bile salts and demonstrated specific adherence properties for colonization of human intestinal mucosa.* L. plantarum 299v has been used in human clinical studies for intestinal
Video photo micrography has been of enormous importance in deciphering the events surrounding the attachment of bacteria to surfaces. The best evidence suggests that attachment occurs as a two-step process. Reversible attachment, which is tenuous and often transient and irreversible attachment that is much more stable. In some cases, following their initial attachment to a surface the bacteria can be seen to be rapidly spinning, vibrating or actually moving across the substrate surface. Spinning is an indication that the cells are attached to the substrate by their flagella, but since the flagellum is now "fixed", the cell body rotates as the flagellar motor continues to rotate. Sometimes, cells in contact with the surface seem to be vibrating, that is they areexhibiting Brownian motion, caused by the constant and random impact of water molecules striking the loosely attached bacterial cell.Videos of Pseudomonas aeruginosa reveal that after initial attachment, cells move over the surface with a ...
Pseudomonas aeruginosa is an opportunistic human pathogen that forms biofilm infections in a wide variety of contexts. Biofilms initiate when bacteria attach to a surface, which triggers changes in gene expression leading to the biofilm phenotype. We have previously shown, for the P. aeruginosa lab strain PAO1, that the self-produced polymer Psl is the most dominant adhesive for attachment to the surface but that another self-produced polymer, Pel, controls the geometry of attachment of these rod-shaped bacteriastrains that make Psl but not Pel are permanently attached to the surface but adhere at only one end (tilting up off the surface), whereas wild-type bacteria that make both Psl and Pel are permanently attached and lie down flat with very little or no tilting (Cooley et al 2013 Soft Matter 9 38716). Here we show that the change in attachment geometry reflects a change in the distribution of Psl on the bacterial cell surface. Bacteria that make Psl and Pel have Psl evenly coating the ...
Compared to asymptomatic UTI, significantly more number of bacteria adhered to the epithelial cells of women with symptomatic UTI (P< 0.001). All cases of UTI had significantly high concentration of urinary IgG antibody to mixed coliform antigens. Asymptomatic UTI cases had higher concentrations of urinary IgG, IgM and IgA antibodies to clinical isolate. Concentration of sIgA level was more in symptomatic UTI. Significant correlation was observed between urinary IgG and adherence of clinical isolate in case of asymptomatic UTI ...
The Asally lab studies the spatio-temporal dynamics of bacterial biofilm formation in vitro and in vivo by taking synthetic and systems biology approaches.
Adhesins are surface structures of bacteria that facilitate their attachment to host cells or non-living materials. New research has advanced our understanding of adhesins, as outlined in a new report ...
Family built after PMID=25023666; The GT101 module of Streptococcus parasanguinis dGT1 catalyzes the transfer of glucose to the branch point of the hexasaccharide O-linked to the serine-rich repeat of the bacterial adhesin Fap1 ...
In article ,2df8d690 at rohmhaas.com,, Kenneth_M_Wiencek at ROHMHAAS.COM (Kenneth M Wiencek) wrote: , Heres a question to ponder... , , What is a biofilm anyway? Is one attached cell a biofilm? 100? , 1000000? A monolayer? Is a 3-D structure a requirement for a , biofilm? .....[edit]....if the characteristics of cells change , immediately upon attachment (a physiological response to being , attached such as increased resistance to antimicrobials), then perhaps , a biofilm could be one attached cell (a biofilm onto itself) It seems to me that combining one attached cell with the exponential growth equation necessarily implies the presence of a large multicell film after not very many generations (or one dead attached cell if the growth rate constant is negative). Single cells are irrelevant except as progenitors for populations. -- R.A.Preston rapr at med.pitt.edu ...
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This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
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"Bacterial Adhesion to Worn Silicone Hydrogel Contact Lenses". Optometry and Vision Science. 85 (7): 520-525. doi:10.1097/OPX. ... "Bacterial adhesion to conventional hydrogel and new silicone hydrogel contact lens materials". Graefes Arch Clin Exp Ophthalmol ... and octyl glucoside used as a lens surfactant significantly decreases bacterial adhesion.[98] These compounds are of particular ... Bacterial keratitis and conjunctivitis. In: Smolin G, Thoft RA, editors. The Cornea. Scientific Foundations and Clinical ...
Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV (June 2002). "Bacterial adhesion to target cells enhanced by shear ... Leukocyte adhesion deficiency[edit]. Main article: Leukocyte adhesion deficiency. Leukocyte adhesion deficiency (LAD) is a ... Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV (June 2002). "Bacterial adhesion to target cells enhanced by shear ... Tight adhesion[edit]. At the same time, chemokines released by macrophages activate the rolling leukocytes and cause surface ...
... of a bacterial cell to its exterior. Secretion is a very important mechanism in bacterial functioning and operation in their ... to the Pseudomonas fluorescens cell adhesion protein LapA of 520 kDa.[7] The best characterized are the RTX toxins and the ... 2009). Bacterial Secreted Proteins: Secretory Mechanisms and Role in Pathogenesis. Caister Academic Press. ISBN 978-1-904455-42 ... Salyers, A. A. & Whitt, D. D. (2002). Bacterial Pathogenesis: A Molecular Approach, 2nd ed., Washington, D.C.: ASM Press. ISBN ...
Bacterial adhesion force quantification by fluidic force microscopy. (2015) Nanoscale, 7 (9), 4070 - 4079. doi:10.1039/ ... By measuring the adhesion of single cells, important information for different topics in biology and materialscience can be ... With FluidFM it is possible to increase the rate in which these experiments can be performed, and even to assess the adhesion ... By raising the probe, the force of the adhesion can be measured with pN resolution. The method to perform a single bacteria ...
Bacterial adhesion is particularly important for oral bacteria. Oral bacteria have evolved mechanisms to sense their ... However, a highly efficient innate host defense system constantly monitors the bacterial colonization and prevents bacterial ... Most of the bacterial species found in the mouth belong to microbial communities, called biofilms, a feature of which is inter- ... Dental plaque is the material that adheres to the teeth and consists of bacterial cells (mainly S. mutans and S. sanguis), ...
Fasciclin I is an insect neural cell adhesion molecule involved in axonal guidance that is attached to the membrane by a GPI- ... Bacterial immunogenic protein MPT70 (1 FAS1 domain). The FAS1 domains of both human periostin and BIgH3 proteins were found to ... Kim JE, Kim SJ, Lee BH, Park RW, Kim KS, Kim IS (October 2000). "Identification of motifs for cell adhesion within the repeated ... Fasciclin 2 Huber O, Sumper M (September 1994). "Algal-CAMs: isoforms of a cell adhesion molecule in embryos of the alga Volvox ...
R. Oliveira; et al., "Hydrophobicity in Bacterial Adhesion", Biofilm community interactions: chance or necessity? (PDF), ... In the next 24 hours, this layer allows the process of bacterial adhesion to occur, with both diatoms and bacteria (e.g. vibrio ... biofilm formation and bacterial adhesion - and macrofouling - attachment of larger organisms. Due to the distinct chemistry and ... demonstrate a high correlation between their resistance to bacterial adhesion and their hydrophobicity.[37] ...
The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for ... homophilic cell adhesion via plasma membrane adhesion molecules. • entry of bacterium into host cell. • protein localization to ... cell adhesion molecule binding. • cadherin binding. • identical protein binding. Cellular component. • cell-cell adherens ... cell adhesion. • extracellular matrix organization. • response to drug. • cellular response to indole-3-methanol. • ...
This is possible due to the bacterial protein FimH, which mediates high adhesion in response to high flow. The lectin domain is ... Catch bonds also play a significant role in bacterial adhesion, most notably in Escherichia coli. E. coli and other bacteria ... Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV (June 2002). "Bacterial adhesion to target cells enhanced by shear ... Using a Laminar Flow System to Explain Shear-Enhanced Bacterial Adhesion. Presented at Int. Conf. Microchannels Minichannels ( ...
Most members of Enterobacteriaceae have peritrichous, type I fimbriae involved in the adhesion of the bacterial cells to their ...
Because of their antimicrobial activity, thiolated polymers are also used as coatings that avoid bacterial adhesion. Thiomers ... "N-acetylcysteine-functionalized coating avoids bacterial adhesion and biofilm formation". Sci. Rep. 7 (1): 17374. doi:10.1038/ ... Interaction with a bacterial membrane model". React. Funct. Polym. 73: 1384-1390. doi:10.1016/j.reactfunctpolym.2013.01.004. ...
"A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity". Phytochemistry. 66 (18): 2281-91. doi: ... In vitro, A-type proanthocyanidins isolated from cranberry juice cocktail demonstrated anti-adhesion activity against E. coli ... and reduction of the risk of urinary tract infection by inhibiting the adhesion of certain bacteria in the urinary tract ...
1994). "CD66 identifies the biliary glycoprotein (BGP) adhesion molecule: cloning, expression, and adhesion functions of the ... The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. ... Carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) also known as CD66d (Cluster of Differentiation 66d), is a ... 2003). "Phosphatidylinositol 3-kinases in carcinoembryonic antigen-related cellular adhesion molecule-mediated internalization ...
Marshall K. (1992). »Biofilms: an overview of bacterial adhesion, activity, and control at surfaces«. ASM News 37: 778-89. ... 1987). »Bacterial biofilms in nature and disease«. Annual Reviews of Microbiology, 41: 435-464 ... Costerton J., Stewart P., Greenberg E. (1999). »Bacterial biofilms: a common cause of persistent infections«. Science, 284(5418 ...
YadA bacterial adhesin protein domain Type V secretion system Virulence factor Cell adhesion Outer membrane Gram negative ... they are a complex that aids adhesion to the ECM. Secretion is one method of transferring substances across the bacterial outer ... Bacteria use TAAs in order to infect their host cells via a process called cell adhesion. TAAs also go by another name, ... Function: Their role is to act as spacers by moving the head domains away from the bacterial cell surface and toward the ...
Simulation of bacterial attraction and adhesion to falling particles in an aquatic environment. Limnol. Oceanogr. 34: 514-530. ... In 2003, a new bacterial species was discovered that swim sideways and respond to differences in oxygen concentration at the ... The gradient-sensing mechanism in bacterial chemotaxis. Proc. Natl. Acad. Sci. U.S.A. 69: 2509 -2512. Adler, J. 1975. ... Mathematical modeling and quantitative characterization of bacterial motility and chemotaxis. In Modeling the Metabolic and ...
... and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a ...
Studies are under way to characterize the bacterial isolates from the outbreak in detail. Physicians around the world should be ... The bacteria contain an adhesion protein for the carbohydrate sequence Gal-1,4Gal. After incubation with various amounts of the ... Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. ... pathogen, magnetic concentration and ATP detection, bacterial levels down to 10^5 cfu could be detected. Sriskandan S, Slater ...
Lpp induces adhesion of neutrophils to human endothelial cells by activating the latter. Seltmann, Guntram; Holst, Otto (2002 ... Silhavy TJ, Kahne D, Walker S (2010). "The bacterial cell envelope". Cold Spring Harbor Perspectives in Biology. 2 (5): a000414 ... Kovacs-Simon A, Titball RW, Michell SL (2011). "Lipoproteins of bacterial pathogens". Infection and Immunity. 79 (2): 548-61. ... The Bacterial Cell Wall. Berlin: Springer. pp. 81-82. ISBN 3-540-42608-6. Dramsi S, Magnet S, Davison S, Arthur M (2008). " ...
Mu XQ, Jiang ZG, Bullitt E (2005). "Localization of a critical interface for helical rod formation of bacterial adhesion P-pili ... Bacterial pili are used in the exchange of genetic material during bacterial conjugation, while a shorter type of appendages ... Although not all bacteria have pili or fimbriae, bacterial pathogens often use their fimbriae to attach to host cells. In Gram- ... Genetic transformation is the process by which a recipient bacterial cell takes up DNA from a neighboring cell and integrates ...
These vesicles were suspected to promote bacterial adhesion to the host epithelial cell surface. Their role in invasion of ... In the prokaryotic gram-negative bacterial cells, membrane vesicle trafficking is mediated via bacterial outer membrane bounded ... In inter-bacterial interactions, OMVs released by Pseudomonas aeruginosa microbes were shown to fuse with outer membrane of ... Halhoul, N.; Colvin, J.Ross (1975). "The ultrastructure of bacterial plaque attached to the gingiva of man". Archives of Oral ...
Bacterial meningitis can also result in gliotic blockage of the aqueduct. In utero infection or infection during infancy could ... This disorder is caused by a point mutation in the gene for neural cell adhesion. Most males born with this have severe ...
Engineering hydrophobin DewA to generate surfaces that enhance adhesion of human but not bacterial cells. Acta Biomaterialia, 8 ...
Leukocyte adhesion to the damaged endothelial wall and abnormal von Willebrand factor (or vWF) release can also contribute to ... Bacterial toxins are the primary cause of one category of thrombotic microangiopathy known as HUS or hemolytic uremic syndrome ... Either of these pathways will result in decreased endothelial thromboresistance, leukocyte adhesion to damaged endothelium, ... bacterial Shiga toxins or endotoxins, antibodies, and drugs; and congenital predisposing conditions, including decreased levels ...
This is also mediated by the presence of few bacterial adhesion sites in the bladder and urethra. Women with these receptors ... Robust fucosyltransferase activity discourages bacterial adherence in the urethra of women. ... who do not have mucosal secretion of the fucosyltransferase enzyme to help block bacterial adherence are more likely to have ...
The anaerobic bacterial species Cutibacterium acnes (formerly Propionibacterium acnes) contributes to the development of acne, ... for the treatment of superficial atrophic acne scars and involves the use of a small needle to loosen the fibrotic adhesions ... These reinforced the idea amongst dermatologists that bacterial growth on the skin plays an important role in causing acne.[179 ... such as bacterial resistance.[194] Oral and topical probiotics are under evaluation as treatments for acne.[195] Probiotics may ...
Bacterial adhesion to target cells enhanced by shear force.. Thomas WE1, Trintchina E, Forero M, Vogel V, Sokurenko EV. ... Surface adhesion of bacteria generally occurs in the presence of shear stress, and the lifetime of receptor bonds is expected ... we show that bacterial attachment to target cells switches from loose to firm upon a 10-fold increase in shear stress applied. ...
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The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and ... Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly( ... reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, ... The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment. ...
Evidence That Focal Adhesion Complexes Power Bacterial Gliding Motility Message Subject. (Your Name) has forwarded a page to ... Evidence That Focal Adhesion Complexes Power Bacterial Gliding Motility. By Tâm Mignot, Joshua W. Shaevitz, Patricia L. ... Evidence That Focal Adhesion Complexes Power Bacterial Gliding Motility. By Tâm Mignot, Joshua W. Shaevitz, Patricia L. ... Unexpectedly, bacteria can move as eukaryotes do, by adhering to the surface via transient adhesion sites that continually ...
Methods of inhibiting bacterial adhesion to medical implants and reducing device-associated infection are effectuated by ... The anti-bacterial activity of this protein molecule relates to its effective inhibition of bacterial adhesion to surfaces of ... Effect of Serum on Bacterial Adhesion. In this example, adhesion studies were conducted to examine the ability of bacteria to ... on bacterial adhesion to a polyurethane surface.. DETAILED DESCRIPTION. Before the present methods for inhibiting bacterial ...
T. R. Garrett, M. Bhakoo, and Z. Zhang, "Bacterial adhesion and biofilms on surfaces," Progress in Natural Science, vol. 18, no ... Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. Khulood Hamid ... In the adhesion assay, the impact of biofilms on cell adhesion to the surface of glass beads was investigated using unwashed ... "Extracellular polymeric substances responsible for bacterial adhesion onto solid surface," FEMS Microbiology Letters, vol. 223 ...
T. R. Garrett, M. Bhakoo, and Z. Zhang, "Bacterial adhesion and biofilms on surfaces," Progress in Natural Science, vol. 18, no ... Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion. Khulood Hamid ... "Extracellular polymeric substances responsible for bacterial adhesion onto solid surface," FEMS Microbiology Letters, vol. 223 ... "Hydrophobic and electrostatic parameters in bacterial adhesion-dedicated to Werner Stumm for his 65th birthday," Aquatic ...
... Khulood Hamid ... "Methicillin-Resistant Staphylococcus aureus Biofilms and Their Influence on Bacterial Adhesion and Cohesion," BioMed Research ...
Adhesion plays a major role in the bacterial lifestyle. Bacteria can adhere to organic and inorganic surfaces, to each other, ... Adhesion plays a major role in the bacterial lifestyle. Bacteria can adhere to organic and inorganic surfaces, to each other, ... Assessing Bacterial Adhesion on an Individual Adhesin and Single Pili Level Using Optical Tweezers ... Protein Folding in Bacterial Adhesion: Secretion and Folding of Classical Monomeric Autotransporters ...
... coli bacterial infection starts with adhesion to a host cell using cell surface expressed adhesion polymers, called adhesion ... These pili, which are vital for bacterial adhesion, thereby serve as a new possible approach in the fight against bacterial ... The mechanics of adhesion polymers and their role in bacterial attachment. Zakrisson, Johan Umeå University, Faculty of Science ... The mechanics of adhesion polymers and their role in bacterial attachment(2863 kB). 252 downloads. ...
Bacterial adhesion in vitro and in silico. München, 03/29/2018. LMU researchers have characterized the physical mechanism that ... Bacterial pathogens have evolved highly effective strategies that enable them to adhere to target cells and niches in the ... The study shows that, thanks to the geometry of the interaction, the adhesion protein forms a dense network of non-covalent ... In order to dissect the adhesion mechanism, the Blue Waters supercomputer at the University of Illinois, with its 900,000 ...
Wet-surface-enhanced ellipsometric contrast microscopy identifies slime as a major adhesion factor during bacterial surface ... Wet-surface-enhanced ellipsometric contrast microscopy identifies slime as a major adhesion factor during bacterial surface ... Slime Mediates Adhesion and Is Deposited by the Motility Complexes During Motility.. Could slime mediate adhesion of the ... This way, the machinery itself may lay its own adhesion substrate, enhancing Myxococcus substrate adhesion and allowing cells ...
BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial ... Bacterial Adhesion. Known as: Adhesion, Bacterial, Adhesions, Bacterial, Bacterial Adhesions Physicochemical property of ... Bacterial Adhesion to Target Cells Enhanced by Shear Force. *Wendy E. Thomas, Elena Trintchina, Manu Forero, V. Vogel, Evgeni V ... Surface thermodynamics of bacterial adhesion.. *Darryl R. Absolom, Francesco Lamberti, Zdenka Policova, Walter Zingg, Carel Jan ...
Abstract: J7.00001 : Catch bonds enable bacterial and cell adhesion under flow. 11:15 AM-11:51 AM. ... How well does our intuition serve us when trying to prevent bacterial infections? If receptor-ligand complexes are being pulled ...
... transepithelial components in the inhibition of bacterial adhesion. The ... ... Randomized and Controlled Clinical Trial to Evaluate Bacterial Adhesion on Multi-Im® Transepithelial Components. 2018-06-19 02: ... Home » Citations » Randomized and Controlled Clinical Trial to Evaluate Bacterial Adhesion on Multi-Im® Transepithelial ... More From BioPortfolio on "Randomized and Controlled Clinical Trial to Evaluate Bacterial Adhesion on Multi-Im® Transepithelial ...
The protein binding was dependent on the broth used for bacterial growth. The binding after growth in brain heart infusion ... Measured surface hydrophobicity correlated well with the bacterial binding strength to the proteins. Streptococcal incubation ...
Bacterial Adhesion to Silicone Hydrogel Lenses H. Zhu; A. Kumar; J. Ozkan; D. Wu; S. Masoudi; R. Bandara; M. Willcox; R. N. ... Within a bacterial type, there were differences in adhesion to lens polymer types. Future studies will examine the effect of ... Bacterial Adhesion to Silicone Hydrogel Lenses You will receive an email whenever this article is corrected, updated, or cited ... Many of these adverse events are related to bacterial adhesion to the lenses. This study aimed, for the first time, to examine ...
We found that NT-H could significantly inhibit bacterial adhesion and biofilm formation on its surface compared with Smooth Ti ... In our previous study, we demonstrated that the gentamicin-loaded nanotubes could dramatically inhibit bacterial adhesion and ... were selected to investigate the bacterial adhesion at 6 h and biofilm formation at 24, 48, and 72 h on the HACC-loaded ... Lin W-T, Zhang Y-Y, Tan H-L, Ao H-Y, Duan Z-L, He G, Tang T-T. Inhibited Bacterial Adhesion and Biofilm Formation on ...
An in vitro study on bacterial growth interactions and intestinal epithelial cell adhesion characteristics of probiotic ... rhamnosus GG both decreased significantly in the presence of P. jensenii 702 compared to their adhesion levels when alone (P , ... However, in most cases these differences were not statistically significant (P , 0.05). Adhesion percentage of Lb. casei 01 and ... The results of adhesion assay showed that when probiotic strains were tested in combination, there was evidence of an ...
In parallel with this increased interest for and understanding of bacterial adhesion, there has been a growth in the ... Resent research within this field has documented the important roles played by glycans for bacterial surface adhesion, either ... have contributed to clarifying the mechanisms underlying bacterial adhesion to glycosylated surfaces in general and mucosal ... Bacterial adhesion is currently the subject of increased interest from the research community, leading to fast progress in our ...
Towards achieving polymeric composites capable of resisting bacterial adhesion and biofilm formation, we prepared five 2,6- ... the resulting composites were tested for their antibacterial potential and were found to effectively resist bacterial ... Bacterial biofilms constitute an extremely resistant form of bacterial colonization with dire health and economical ... Preparation of Polyester-Based Metal-Cross Linked Polymeric Composites as Novel Materials Resistant to Bacterial Adhesion and ...
... of a novel marine bacterial polysaccharide EPS11 which exerts its cytotoxic effects through affecting cancer cell adhesion and ... Firstly, we found that EPS11 could significantly affect cell proliferation and block cell adhesion in A549 cells. We further ... and the inhibitory tendency is very consistent with that observed in the cell adhesion assay, which confirms that filiform ... structures play important roles in modulating cell adhesion. Moreover, we showed that EPS11 induces apoptosis of A549 cells ...
... ... Bacterial adhesins affected were identified by overlay assay with immobilized ligands. I-125-radiolabeled F2 served for binding ... Functional testing of F2 was performed by semiquantitative in situ adhesion assay on sections of human gastric mucosa and by ... F2 had no cytotoxic effects against H. pylori and AGS cells; but inhibited bacterial binding to human gastric cells. F2 ...
Although there are several examples of effective strategies to prevent bacterial adhesion, the effect of the wetting properties ... there has been a tremendous increase in research on antibacterial surface coatings as an alternative strategy against bacterial ... Mussel-inspired coatings with tunable wettability, for enhanced antibacterial efficiency and reduced bacterial adhesion ... Mussel-inspired coatings with tunable wettability, for enhanced antibacterial efficiency and reduced bacterial adhesion M. Li, ...
Molecular mechanisms modulating host epithelial integrity in response to bacterial adhesion at University of Birmingham, listed ... Molecular mechanisms modulating host epithelial integrity in response to bacterial adhesion University of Birmingham. , School ... Bacterial adhesion is one of the first and most crucial steps during the interaction between bacteria and host organisms. This ... We recently identified Multivalent Adhesion Molecules (MAMs) as a new and wide-spread family of bacterial adhesins mediating ...
  • The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment. (mdpi.com)
  • Thirdly, adhesion assay was used to calculate percentages of the bacterial adhesion to enamel particles with or without saliva and/or heating. (nus.edu.sg)
  • Resent research within this field has documented the important roles played by glycans for bacterial surface adhesion, either through interaction with lectins or with other glycans. (mdpi.com)
  • Abstract Bacterial endotoxin (LPS) adhesion to orthodontic brackets is a known contributing factor to inflammation of the adjacent gingival tissues . (bvsalud.org)
  • LMU researchers have characterized the physical mechanism that enables a widespread bacterial pathogen to adhere to the tissues of its human host. (uni-muenchen.de)
  • Bacterial outer membrane vesicles Exocytosis Host-pathogen interaction Host-pathogen interface Secretory pathway Vesicle (Biology and Chemistry) Virulence Thery, C. (wikipedia.org)
  • It is a human pathogen that causes the disease mycoplasma pneumonia, a form of atypical bacterial pneumonia related to cold agglutinin disease. (wikipedia.org)
  • Bordetella dermonecrotic toxin (DNT) stimulates the assembly of actin stress fibres and focal adhesions by deamidating or polyaminating Gln63 of the small GTPase Rho. (wikipedia.org)
  • Streptococcus pneumoniae (pneumococcus) is a potential cause of bacterial endophthalmitis in humans that can result in ocular morbidity. (nih.gov)
  • medical citation needed] In cases of subacute bacterial endocarditis, the causative organism (streptococcus viridans) needs a previous heart valve disease to colonize. (wikipedia.org)
  • However, their mechanical functions and the advantage of specific mechanical functions, especially in the initial attachment process, have not yet been fully understood.In this work, a detailed description of the pili mechanics and their role during cell adhesion is presented. (diva-portal.org)
  • Finally, the resulting composites were tested for their antibacterial potential and were found to effectively resist bacterial attachment and growth. (mdpi.com)
  • A number of approaches have been used, in both in vitro and in vivo experimental models, to inhibit bacterial attachment to temporally expressed host receptors. (microbiologyresearch.org)
  • Although there are several examples of effective strategies to prevent bacterial adhesion, the effect of the wetting properties on the coating was rarely considered as a crucial factor. (rsc.org)
  • However, in order to develop such chemicals, better understanding of these pili is needed.Optical tweezers (OT) can measure and apply forces up to a few hundred pN with sub-pN force resolution and have shown to be an excellent tool for investigating mechanical properties of adhesion pili. (diva-portal.org)
  • Deletion of the pilV and pilX genes led to a decrease in the number, but not length, of pili displayed on the bacterial surface indicating a role in the initiation of pilus biogenesis. (embopress.org)
  • Type IV pili are part of a larger group of bacterial machineries. (embopress.org)
  • Type IV pili and related structures are thus one of the building blocks of the archeal and bacterial world. (embopress.org)
  • In the last two decades, it was discovered that the archaeal flagella, although functionally similar to bacterial and eukaryotic flagella, structurally resemble bacterial type IV pili. (wikipedia.org)
  • Moreover, the structure of the archaellum filament resembles type IV pili as it has no central lumen excluding the possibility that it might assembled in a similar fashion like bacterial flagella via a type III secretion system. (wikipedia.org)
  • Does upregulated host cell receptor expression provide a link between bacterial adhesion and chronic respiratory disease? (tcd.ie)
  • O'Toole R.F, Shukla S.D, Walters E.H, Does upregulated host cell receptor expression provide a link between bacterial adhesion and chronic respiratory disease? (tcd.ie)
  • The measurement of Bacillus mycoides spore adhesion using atomic force microscopy, simple counting methods, and a spinning disk technique. (microbiologyresearch.org)
  • This invention relates to a method for coating a medical device comprising the steps of applying to at least a portion of the surface of said medical device, an antimicrobial coating layer and a non-pathogenic bacterial coating layer, wherein the antimicrobial and non-pathogenic bacterial coating layers. (google.com.au)
  • Investigation of the effect of adhesion contact time revealed that PDA coatings are less effective at preventing bioadhesion when the contact time is prolonged to 2-5 s, or when the membranes are exposed to bacterial suspensions under stirring. (umn.edu)
  • The compacted membranes were also employed as substrata for monitoring the initial adhesion of Ps. (ucd.ie)
  • These differences in initial cell adhesion rates demonstrate that choice of laboratory water can significantly impact the results of bacterial adhesion on NF membranes. (ucd.ie)
  • A type of junctional complex, they are localized spot-like adhesions randomly arranged on the lateral sides of plasma membranes. (wikipedia.org)
  • In this thesis, an experimental setup of a force measuring optical tweezers system and the results of a number of biomechanical investigations of adhesive bacterial organelles are presented. (diva-portal.org)
  • Target prediction and KEGG pathway analysis indicated that these ncRNAs are closely related to pathways associated with in vitro adhesion, and our results indicated that chemical stress-induced reductions in the adhesion ability of V. alginolyticus might be due to the perturbation of ncRNA expression. (frontiersin.org)