A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.
A species of gram-positive bacteria that is a common soil and water saprophyte.
Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.
The reproductive elements of lower organisms, such as BACTERIA; FUNGI; and cryptogamic plants.
A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
Bacteriocins elaborated by mutant strains of Bacillus megaterium. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same species.
D-Glucose:1-oxidoreductases. Catalyzes the oxidation of D-glucose to D-glucono-gamma-lactone and reduced acceptor. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.47; EC 1.1.1.118; EC 1.1.1.119 and EC 1.1.99.10.
Proteins found in any species of bacterium.
A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.
The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.
A species of bacteria that causes ANTHRAX in humans and animals.
A glucose dehydrogenase that catalyzes the oxidation of beta-D-glucose to form D-glucono-1,5-lactone, using NAD as well as NADP as a coenzyme.
A class of chemicals derived from barbituric acid or thiobarbituric acid. Many of these are GABA MODULATORS used as HYPNOTICS AND SEDATIVES, as ANESTHETICS, or as ANTICONVULSANTS.
A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.
A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
A genus of endospore-forming bacteria in the family Planococcaceae. The species are strict or facultative anerobes.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
The functional hereditary units of BACTERIA.
A chlorinated bisphenol antiseptic with a bacteriostatic action against Gram-positive organisms, but much less effective against Gram-negative organisms. It is mainly used in soaps and creams and is an ingredient of various preparations used for skin disorders. (From Martindale, The Extra Pharmacopoeia, 30th ed, p797)
An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Widely distributed enzymes that carry out oxidation-reduction reactions in which one atom of the oxygen molecule is incorporated into the organic substrate; the other oxygen atom is reduced and combined with hydrogen ions to form water. They are also known as monooxygenases or hydroxylases. These reactions require two substrates as reductants for each of the two oxygen atoms. There are different classes of monooxygenases depending on the type of hydrogen-providing cosubstrate (COENZYMES) required in the mixed-function oxidation.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Provision of physical and biological barriers to the dissemination of potentially hazardous biologically active agents (bacteria, viruses, recombinant DNA, etc.). Physical containment involves the use of special equipment, facilities, and procedures to prevent the escape of the agent. Biological containment includes use of immune personnel and the selection of agents and hosts that will minimize the risk should the agent escape the containment facility.
A species of parasitic OOMYCETES in the family Peronosporaceae that is the causative agent of late blight of potato.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)
Systematic identification, development, organization, or utilization of educational resources and the management of these processes. It is occasionally used also in a more limited sense to describe the use of equipment-oriented techniques or audiovisual aids in educational settings. (Thesaurus of ERIC Descriptors, December 1993, p132)
Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.
Beaches, both natural and man-made, used for bathing and other activities.
A self-learning technique, usually online, involving interaction of the student with programmed instructional materials.

Microbial catabolism of vanillate: decarboxylation to guaiacol. (1/655)

A novel catabolic transformation of vanillic acid (4-hydroxy-3-methoxybenzoic acid) by microorganisms is reported. Several strains of Bacillus megaterium and a strain of Streptomyces are shown to convert vanillate to guaiacol (o-methoxyphenol) and CO2 by nonoxidative decarboxylation. Use of a modified most-probable-number procedure shows that numerous soils contain countable numbers (10(1) to 10(2) organisms per g of dry soil) of aerobic sporeformers able to convert vanillate to guaiacol. Conversion of vanillate to guaiacol by the microfloras of most-probable-number replicates was used as the criterion for scoring replicates positive or negative. Guaiacol was detected by thin-layer chromatography. These results indicate that the classic separations of catabolic pathways leading to specific ring-fashion substrates such as protocatechuate and catechol are often interconnectable by single enzymatic transformations, usually a decarboxylation.  (+info)

Metabolism and the triggering of germination of Bacillus megaterium. Concentrations of amino acids, organic acids, adenine nucleotides and nicotinamide nucleotides during germination. (2/655)

A considerable amount of evidence suggests that metabolism of germinants or metabolism stimulated by them is involved in triggering bacterial-spore germination. On the assumption that such a metabolic trigger might lead to relatively small biochemical changes in the first few minutes of germination, sensitive analytical techniques were used to detect any changes in spore components during the L-alanine-triggered germination of Bacillus megaterium KM spores. These experiments showed that no changes in spore free amino acids or ATP occurred until 2-3 min after L-alanine addition. Spores contained almost no oxo acids (pyruvate, alpha-oxoglutarate, oxaloacetate), malate or reduced NAD. These compounds were again not detectable until 2-3 min after addition of germinants. It is suggested, therefore, that metabolism associated with these intermediates is not involved in the triggering of germination of this organism.  (+info)

Metabolism and the triggering of germination of Bacillus megaterium. Use of L-[3H]alanine and tritiated water to detect metabolism. (3/655)

L-[2,3-3H]Alanine was used to probe for metabolism of alanine during triggering of germination of spores of Bacillus megaterium KM. No detectable incorporation of label into any compound, including water, was found, indicating that any metabolism involving the alanine germinant must be at a very low rate and also that alanine racemase is absent from spores of this strain. Spores were germinated in 3H2O to find if any of the many metabolic reactions causing irreversible incorporation of 3H into reaction products took place during triggering of germination. No incorporation was detected until 2-3 min after addition of germinants. It is therefore concluded that a wide variety of metabolic routes, including glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway and amino acid metabolism are either not involved in the reactions causing the triggering of germination or operate at an extremely low rate during this process.  (+info)

Adjuvant effect of a peptidoglycan attached covalently to a synthetic antigen provoking anti-phage antibodies. (4/655)

The synthetic antigen denoted P2-A--L, comprising the fragment P2 of the coat protein of MS-2 coliphage attached to multichain poly-DL-alanine, served for the immunization of guinea-pigs. Immunization was carried out either in phosphate buffered saline (PBS) or in Freund's incomplete adjuvant (FIA) in the presence or absence of a small molecular weight peptidoglycan prepared from Bacillus megaterium, which was checked for its adjuvant effect. The various antisera were assessed by their capacity to neutralize MS-2 bacteriophage viability. When injected in PBS or FIA, P2-A--L did not elicit any measurable anti-phage activity. Addition of the peptidoglycan by simple mixing did not bring about a significant increase in antibody production. However, when the peptidoglycan was chemically linked to the P2-A--L conjugate, it had a marked adjuvant effect when the material was administered in FIA, almost identical to the extent of the effect of Freund's complete adjuvant.  (+info)

Purification and characterization of additional low-molecular-weight basic proteins degraded during germination of Bacillus megaterium spores. (5/655)

Dormant spores Bacillus megaterium contained a group of low-molecular-weight (5,000 to 11,000) basic (pI greater than 9.4) proteins (termed D, E, F, and G proteins) which could be extracted from disrupted spores with strong acids. These proteins were distinct from the previously described A, B, and C proteins which are degraded during spore germination. However, the D, E, F, and G proteins were also rapidly degraded during spore germination, accounting for 10 to 15% of the protein degraded. Proteins similar to the D, E, F, and G species were also present in spores of other bacterial species. In B. megaterium, the D, E, F, and G proteins were low or absent (less than 15% of the spore level) in vegetative and young sporulating cells and appeared only late in sporulation. The D, E, F, and G proteins were purified to homogeneity, and all contained a high percentage of hydrophilic amino acids; one protein (G) contained 31% basic amino acids and also contained tryptophan. All four proteins were rapidly degraded in vitro by dormant spore extracts. Two proteins (D and F) were degraded in vitro by the previously described spore protease which initiates degradation of the A, B, and C proteins in vivo; the spore enzyme (s) degrading proteins E and G have not been identified.  (+info)

Membrane-bound DD-carboxypeptidase and transpeptidase activities from Bacillus megaterium KM at pH 7. General properties, substrate specificity and inhibition by beta-lactam antibiotics. (6/655)

1. The membranes from Bacillus megaterium KM contained a DD-carboxypeptidase with optimum activity under the following conditions: pH 7; ionic strength, 1.3 M; temperature, 40 degrees C and below 20 degrees C. It did not require any divalent cation, but was inactivated by Cu2+ and Hg2+. It was stimulated by 2-mercaptoethanol and low concentrations of p-chloromercuribenzoate. 2. The membrane preparation also catalyzed a simple transpeptidation reaction using as carboxyl acceptors D-alanine or glycine. 3. The conditions for optimum activity, temperature-inactivation, temperature-dependence of the activity, carboxyl donor specificity, sensitivity to beta-lactam antibiotics, and insensitivity to potential peptide inhibitors of both enzyme activities, was identical. The DD-carboxypeptidase showed inhibition by D-alanine and Ac2-L-Lys-D-Ala. 4. The inhibition by beta-lactam antibiotic was reversible for both enzymic activities and the time-dependence for their recovery was identical. 5. The DD-carboxypeptidase was very sensitive to changes in the configuration and size of the side-chains of the C-terminal dipeptide of the substrate. Amino acid residues at the C-terminus that precluded the peptide from being a DD-carboxypeptidase substrate were not acceptors in the transpeptidation reaction. Dipeptides were not acceptors for the 'model transpeptidase'. 6. It is suggested that both activities are catalysed by the same enzyme molecule, whose physiological role is not the formation of peptide crosslinks during peptidoglycan biosynthesis.  (+info)

Effect of beta-lactam antibiotics on in vitro peptidoglycan cross-linking by a particulate fraction from Escherichia coli K-12 and Bacillus megaterium KM. (7/655)

The binding constants of several beta-lactam antibiotics towards penicillin-binding components in Escherichia coli K-12 (Spratt, Eur. J. Biochem. 72:341-352, 1977) and the antibiotic concentrations required to inhibit the peptidoglycan transpeptidase of E. coli 50% were compared. Penicillin-binding component 1B may have been the transpeptidase working in vitro. The structure-activity relationships of beta-lactam antibiotics and the mechanisms of action in E. coli and Bacillus megaterium are discussed.  (+info)

Cloning of oxetanocin A biosynthetic and resistance genes that reside on a plasmid of Bacillus megaterium strain NK84-0128. (8/655)

Bacillus megaterium strain NK84-0218 produces a potent antiviral antibiotic, oxetanocin A, which has an oxetanosyl-N-glycoside linkage to an adenine moiety. However, the oxetanocin A productivity of the original strain was unstable and low. In this study, oxetanocin A productivity and resistance was shown to be lost simultaneously when a 51.5-kb plasmid, pOXT1, was cured during cultivation. The deficiency of oxetanocin A productivity and resistance was restored by re-introduction of the pOXT1 plasmid into the cured strain. By a cloning experiment it was shown that a 6.8-kb BglI-D fragment of the pOXT1 plasmid was responsible for oxetanocin A productivity and resistance.  (+info)

Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. Of the total spore Zn2+ pool 25% is released from non-heat-activated spores within 4 min of triggering germination. During this period only 10% of the spore population becomes irreversibly committed to germinate. The investigation of a putative role for Zn2+ in the germination trigger mechanism has established a relationship between the rate and extent of Zn2+ release and the stimulation of spore germination by heat activation. Furthermore, a correlation can be demonstrated between the extent of zinc release from spore populations and the time required to obtain 50% commitment of these populations to germinate over a wide temperature range. These findings have been used to expand a recently published model for the triggering of bacterial spore germination. ...
An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This stu-dy aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus megaterium, and the bacterium that was observed to thrive in alkaline conditions (pH 7.0−14.0), supplemented with varying concentrations of 2,2-DCP (from 20 to 60 mM). Growth of strain BHS1 was exceptional in 40 mM of 2,2-DCP at pH 9, corresponding to a cell doubling time of 17.7 hour, whereas was fully inhibited at 50 mM 2,2-DCP. Since halogen-ated pollutants can make their way into highly alkaline ...
Terpenoids, also known as isoprenoids, are a large class of natural products consisting of isoprene (C5) units. There are two biosynthetic pathways, the mevalonate pathway [MD:M00095] and the non-mevalonate pathway or the MEP/DOXP pathway [MD:M00096], for the terpenoid building blocks: isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The action of prenyltransferases then generates higher-order building blocks: geranyl diphosphate (GPP), farsenyl diphosphate (FPP), and geranylgeranyl diphosphate (GGPP), which are the precursors of monoterpenoids (C10), sesquiterpenoids (C15), and diterpenoids (C20), respectively. Condensation of these building blocks gives rise to the precursors of sterols (C30) and carotenoids (C40). The MEP/DOXP pathway is absent in higher animals and fungi, but in green plants the MEP/DOXP and mevalonate pathways co-exist in separate cellular compartments. The MEP/DOXP pathway, operating in the plastids, is responsible for the formation of essential oil ...
Bacillus megaterium ATCC ® 14581™ Designation: TypeStrain=True Application: VITEK 2 BCL Comprehensive QC Set Quality control strain
Mystical Biotech Pvt. Ltd. is a growing Manufacturer, Supplier, Exporter of Bacillus megaterium BM-706 based in Banagaluru, Karnataka, India. We are offering high quality Bacillus megaterium BM-706. Contact us now.
Bacillus megaterium is a microorganism widely used in industrial biotechnology for production of enzymes and recombinant proteins, as well as in bioleaching processes. Precise understanding of its metabolism is essential for designing engineering strategies to further optimize B. megaterium for biotechnology applications. Here, we present a genome-scale metabolic model for B. megaterium DSM319, iJA1121, which is a result of a metabolic network reconciliation process. The model includes 1709 reactions, 1349 metabolites, and 1121 genes. Based on multiple-genome alignments and available genome-scale metabolic models for other Bacillus species, we constructed a draft network using an automated approach followed by manual curation. The refinements were performed using a gap-filling process. Constraint-based modeling was used to scrutinize network features. Phenotyping assays were performed in order to validate the growth behavior of the model using different substrates. To verify the model accuracy,
Genus and Species: Bacillus megaterium Domain: Prokaryote Optimal Growth Medium: Nutrient Agar Optimal Growth Temperature: 30° C Package: Tube Biosafety Level: 1 Gram Stain: Gram-Positive Shape: Bacillus (rod-shaped)
Vitamin B12 (cobalamin) is one of Natures most complex small molecules. It is a cobalt-containing modified tetrapyrrole that plays a number of key metabolic roles in many prokaryotic systems and is also essential to the biochemistry of higher animals. However, it is made by only certain bacteria, requiring around thirty enzymatic steps for its complete de novo construction. Surprisingly, the nutrient is synthesised by one of two related, though genetically distinct, pathways that represent aerobic or anaerobic routes. The anaerobic pathway has remained poorly characterised due to the instability of the pathway intermediates to oxygen and the low activity of enzymes, and is the focus of the research reported in this thesis. The Gram-positive aerobe Bacillus megaterium has previously been used for the commercial production of cobalamin and has a complete anaerobic pathway. Several genes, (termed chi for 9,obinamide biosynthesis) from the cobalamin biosynthetic pathway have been cloned and ...
TY - JOUR. T1 - Microsome-mediated mutagenicity of aflatoxin B1 in Bacillus megaterium SJ-6 his-. AU - Buch, J. K.. AU - Buch, Shilpa. AU - Dave, P. J.. PY - 1982/4. Y1 - 1982/4. UR - http://www.scopus.com/inward/record.url?scp=0020028778&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0020028778&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0020028778. VL - 13. SP - 349. EP - 352. JO - FEMS Microbiology Letters. JF - FEMS Microbiology Letters. SN - 0378-1097. IS - 4. ER - ...
BioAssay record AID 1083218 submitted by ChEMBL: Antibacterial activity against Bacillus megaterium clinical isolate at 100 ug/ml after 24 hr by disk diffusion method.
During the last decade Bacillus megaterium was systemically developed for the gram per liter production of recombinant proteins. In contrast to the usual bacterial gene expression scenario, recombinant protein production systems often rely on multicopy plasmid encoded promoters, repressors and target genes. During production of the green fluorescent model protein (GFP) as a model culture heterogeneity was observed at the single cell level using time-lapse microscopy. The two subpopulations (producing versus low-producing) were separated by cell sorting and initially analyzed for limiting components of the expression system via quantitative RT-PCR. Further cultivation of both subpopulations revealed a systematic reajustment of their subpopulation distributions, indicationg bistability. An initial model was deduced as working hypothesis to identify the molecular basis for the observed phenotype and to improve our mathematical model ...
Bacillus megaterium bacterial bulture for microbiology laboratory studies is large, easily observed, spore-forming variably motile rods found in soil and water.
Bacillus megaterium strain ATCC 14581 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial ...
|p|Terraflora™ is a novel broad-spectrum synbiotic formulated with a combination of spore form probiotics, and advanced, food-based, ancient prebiotics designed for robust support of gastrointestinal health.|/p| |p|Terraflora’s innovative strain combination introduces Ribospore™ (Bacillus pumilus) and Bacillus megaterium EM144™, microorganisms scientifically proven to effectively produce highly bioavailable antioxidants and riboflavin, right at the site of absorption. Riboflavin is an essential nutrient for maintaining a healthy digestive tract.|/p| |p|Suggested Use: Take 1-2 capsules per day with or without food, or as recommended by your healthcare professional.|br /||br /|If you are pregnant, nursing, or have a medical condition, consult your healthcare practitioner before use.|/p| |p|Ingredients:|/p| |p|Probiotic Bio-Complex: 1 Billion Viable Cells per serving|/p| |blockquote|Ribospore™ (Bacillus pumilus)|/blockquote| |blockquote|Bacillus megaterium EM144™|
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
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কাঁঠাল ফলটি যেমন মজাদার তেমন তার বিচিগুলোও হয় সুস্বাদু । তাই আমরা অনেকেই কাঁঠালের বিচি খেয়ে থাকে তরকারির সাথে বা তেলে ভেজে । কিন্তু সুস্বাদুর পাশাপাশি এটি যে বেশকিছু গুনাগুণ সম্পন্ন টা আমরা অনেকেই জানি না । চলুন দেখে নেয়া যাক কাঁঠালের বিচির গুনাগুণগুলো । ১। এতে আছে এন্টি অক্সিডেন্ট । এন্টি … [Read more...] ...
Penicillin G acilase (PGA) is a key enzyme in the industrial production of β-lactamic antibiotics. In this work, it was studied the production of PGA from Escherichia coli by recombinant E. coli and from Bacillus megaterium by recombinant B. megaterium. In E. coli, the enzyme accumulates in the periplasmic cell space, B. megaterium secretes PGA, what may reduce downstream costs. With E.coli, the study has begun at the expression step. Using a donated recombinant microorganism, it was studied, in shake flasks cultures, the influence of temperature (ranging from 18 to 28°C) during induction phase in the PGA production. High level expression of PGA E. coli was detected at 20°C, which was 4-fold superior than the volumetric enzymatic activity reached at 28°C. Fed-batch cultures were conducted with glycerol as carbon source, using both defined and complex media as well as IPTG and lactose as inducers. Final biomass concentrations of 100 gDCW/L and 120 gDCW/L and enzymatic activity 210000 and ...
P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been extensively studied over a period of almost forty years. The enzyme has been redesigned to catalyse the oxidation of non-natural substrates as diverse as pharmaceuticals, terpenes and gaseous alkanes using a variety of engineering strat
Este trabalho teve por objetivo a purificação e a caracterização cinética da lipase selecionada de Bacillus megaterium e a verificação do seu potencial para utilização em biocatálise em meio de solventes orgânicos. Dentre ...
wearyellowforseth (1) 2joy-21 (1) A01 (2) A02 (2) A19 (1) A34 (3) A40 (1) acryl (13) acrylverf (100) Alcohol inks (19) Apipila-L18 (5) B.03 Mind Blown (4) B01 (1) B08 (1) B11 (1) B44 (1) Big SdP-B (17) Big SdP-B2 (2) Big SdP-G (7) Big SdP-K (13) Big SdP-O (7) Big SdP-T (2) Big SdP-V (4) birthday collab (1) BM 03 (1) BM-01 (1) BM-02 (4) BM-04 (5) BM-08 (1) BM-09 (1) BM-13 (4) BM-14 (2) BM-167 (1) BM-18 (1) BM-201 (2) BM-205 (2) BM-208 (2) BM-212 (1) BM-213 (1) BM-214 (2) BM-215 (1) BM-221 (2) BM-223 (1) BM-224 (3) BM-225 (3) BM-301 (1) BM-304 (1) BM-305 (5) BM-307 (2) BM-308 (1) BM-309 (3) BM-311 (1) BM-312 (1) BM-314 (2) BM-315 (1) BM-317 (1) BM-318 (3) BM-321 (3) BM-322 (1) BM-323 (1) BM-324 (2) BM-408 (1) BM-409 (1) BM-412 (1) BM-413 (1) BM-414 (1) BM-415 (1) BM-416 (1) BM-422 (1) BM-603 (1) BM-604 (1) BM-607 (1) BM-613 (1) BM-619 (1) BM-708 (1) BM-710 (1) bm-717 (1) BM-719 (1) BM-720 (1) BM-H01 (1) BM-H03 (3) BM-H04 (1) BM-H05 (1) BM-H06 (2) BM-H07 (1) BM-H08 (1) BM-H09 (1) BM-H12 (1) BM-H13 ...
wearyellowforseth (1) 2joy-21 (1) A01 (2) A02 (2) A19 (1) A34 (3) A40 (1) acryl (13) acrylverf (100) Alcohol inks (19) Apipila-L18 (5) B.03 Mind Blown (4) B01 (1) B08 (1) B11 (1) B44 (1) Big SdP-B (17) Big SdP-B2 (2) Big SdP-G (7) Big SdP-K (13) Big SdP-O (7) Big SdP-T (2) Big SdP-V (4) birthday collab (1) BM 03 (1) BM-01 (1) BM-02 (4) BM-04 (5) BM-08 (1) BM-09 (1) BM-13 (4) BM-14 (2) BM-167 (1) BM-18 (1) BM-201 (2) BM-205 (2) BM-208 (2) BM-212 (1) BM-213 (1) BM-214 (2) BM-215 (1) BM-221 (2) BM-223 (1) BM-224 (3) BM-225 (3) BM-301 (1) BM-304 (1) BM-305 (5) BM-307 (2) BM-308 (1) BM-309 (3) BM-311 (1) BM-312 (1) BM-314 (2) BM-315 (1) BM-317 (1) BM-318 (3) BM-321 (3) BM-322 (1) BM-323 (1) BM-324 (2) BM-408 (1) BM-409 (1) BM-412 (1) BM-413 (1) BM-414 (1) BM-415 (1) BM-416 (1) BM-422 (1) BM-603 (1) BM-604 (1) BM-607 (1) BM-613 (1) BM-619 (1) BM-708 (1) BM-710 (1) bm-717 (1) BM-719 (1) BM-720 (1) BM-H01 (1) BM-H03 (3) BM-H04 (1) BM-H05 (1) BM-H06 (2) BM-H07 (1) BM-H08 (1) BM-H09 (1) BM-H12 (1) BM-H13 ...
Rain long foretold takes a long time to pass; if it arrives on short notice, it soon will pass. For the folks I go out collecting with all hikes, digs and kayak trips are rain or shine. Safety is always top of mind and prepping for the weather is paramount. Keep yourself safe whatever part of the world you choose to explore. For forecasts of marine weather in Vancouver call 604.666.3655 or visit http://www.weatheroffice.com/ ...
Shikimic acid, the sole chemical building block for the antiviral drug oseltamivir (Tamiflu®), is one of the potent pharmaceutical intermediates with three chiral centers. Here we report a metabolically engineered recombinant Bacillus megaterium strain with aroE (shikimate dehydrogenase) overexpression for the production of shikimic acid. In a 7 L bioreactor, 4.2 g/L shikimic acid was obtained using the recombinant strain over 0.53 g/L with the wild type. The enhancement of total shikimate dehydrogenase activity was 2.13-fold higher than the wild type. Maximum yield of shikimic acid (12.54 g/L) was obtained with fructose as carbon source. It was isolated from the fermentation broth using amberlite IRA-400 resin and 89 % purity of the product was achieved. This will add up a new organism in the armory for the fermentation based production which is better over plant based extraction and chemical synthesis of shikimic acid.
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ID BACMD_1_PE1001 STANDARD; PRT; 158 AA. AC BACMD_1_PE1001; D5DBI6; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein; (BACMD_1.PE1001). GN OrderedLocusNames=BMD_1107; OS BACILLUS MEGATERIUM DSM 319. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OX NCBI_TaxID=592022; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BACMD_1.PE1001. CC Bacillus megaterium DSM 319 chromosome, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:D5DBI6_BACMD CC -!- GENE_FAMILY: HOG000161542 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D5DBI6; -. DR EMBL; CP001982; ADF37968.1; -; Genomic_DNA. DR RefSeq; YP_003596318.1; NC_014103.1. DR EnsemblBacteria; EBBACT00000421253; EBBACP00000414878; EBBACG00000423985. DR GeneID; 9116498; -. DR GenomeReviews; CP001982_GR; BMD_1107. DR KEGG; bmd:BMD_1107; -. DR GeneTree; EBGT00050000004518; -. DR ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Bacillus sp. SW1-2 producing poly glutamic acid (PGA), locally isolated from Eastern province in Saudi Arabia, was characterized and identified based on 16S rRNA gene sequencing. Phylogenetic analysis revealed its closeness to Bacillus megaterium. The homopolymer consists mainly of glutamic as indicated in the analysis of amino acid. Preliminary optimization of PGA production, through a series of one-variable-at-a-time (OVAT) experiments, revealed maximum PGA production of 10.5 (g/L). Statistically based experiments were applied to optimize culture conditions for production of PGA. Effect of 15 variables were examined for their significance on PGA production using Plackett-Burman factorial design. Among those variables; Na-citrate, (NH4)2SO4, glutamic acid and CaCl2.2H2O were most significant variables that encouraged PGA production, while those negatively affected were; glycerol, glucose and phosphate buffer. Significant parameters were further investigated using Box-Behnken design to define ...
1RWB: Cooperative effect of two surface amino acid mutations (Q252L and E170K) in glucose dehydrogenase from Bacillus megaterium IWG3 on stabilization of its oligomeric state.
Streptococcus pyrogenes ( MIC = 100 microg/ml ), Bacillus cereus T ( MIC > 100 microg/ml ), Bacillus megaterium ( MIC = 5 microg/ml ), Bacillus subtilis ( MIC = 5 microg/ml ), Staphylococcus aureus ( MIC > 100 microg/ml ...
In the paper by Waring and Makoff, Breakdown of Pulse-Labeled Ribonucleic Acid and Polysomes in Bacillus megaterium: Actions of Streptolydigin, Echinomycin, and Triostins, Vol. 10, No. 2, March 1974, pp. 214-224, Figs. 1 and 3 were transposed. The legends are correct on the pages where they stand, but the illustration printed as Fig. 1 should appear on p. 219 and the one printed as Fig. 3 should appear on p. 217.. ...
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, and also to enable them to buoy the plastic aggregates. This module requires driving the expression of a gas vesicle gene cluster. Gas vesicles are formed within the cell, and are hollow spaces surrounded by a wall of hydrophobic protein. These gas vesicles are permeable to gases, which diffuse into the gas vesicles, increasing its partial pressure, thereby increasing buoyancy. Gas vesicles are found largely in cyanobacteria and halophillicarchea, where they are necessary to access oxygen and light. Their formation is driven by a number of genes required for production and regulation. We are using the gas vesicle gene to attribute buoyancy to our cells so that they float on to the surface of the ocean. Our particular cluster -known as GVP is extracted from Bacillus Megaterium which contains 14 putative gens gvp-A,-P,-Q,-B,-R,-N,-F,-G,-L,-S,-K,-J,-T ...
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, and also to enable them to buoy the plastic aggregates. This module requires driving the expression of a gas vesicle gene cluster. Gas vesicles are formed within the cell, and are hollow spaces surrounded by a wall of hydrophobic protein. These gas vesicles are permeable to gases, which diffuse into the gas vesicles, increasing its partial pressure, thereby increasing buoyancy. Gas vesicles are found largely in cyanobacteria and halophillicarchea, where they are necessary to access oxygen and light. Their formation is driven by a number of genes required for production and regulation. We are using the gas vesicle gene to attribute buoyancy to our cells so that they float on to the surface of the ocean. Our particular cluster -known as GVP is extracted from Bacillus Megaterium which contains 14 putative gens gvp-A,-P,-Q,-B,-R,-N,-F,-G,-L,-S,-K,-J,-T ...
Raghupathy, Y and Natarajan, KA and Srivastava, Chandan (2016) Anti-corrosive and anti-microbial properties of nanocrystalline Ni-Ag coatings. In: MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 206 . pp. 1-8. Raghupathy, Y and Natarajan, KA and Srivastava, Chandan (2016) Anti-corrosive and anti-microbial properties of nanocrystalline Ni-Ag coatings. In: MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 206 . pp. 1-8. Natarajan, KA (2016) Biomineralization and Biobeneficiation of Bauxite. In: TRANSACTIONS OF THE INDIAN INSTITUTE OF METALS, 69 (1). pp. 15-21. Devi, Karthiga K and Natarajan, KA (2015) Isolation and characterization of a bioflocculant from Bacillus megaterium for turbidity and arsenic removal. In: MINERALS & METALLURGICAL PROCESSING, 32 (4). pp. 222-229. Devi, Karthiga K and Natarajan, KA (2015) Production and characterization of bioflocculants for mineral processing applications. In: INTERNATIONAL JOURNAL OF MINERAL ...
If 7 want alot of chiten kill matisis no matter what u use youll usually get at least a hundred. If you use a megatherium u got almost 1000 with a tame. U got a breed one your looking at 1500 each mantis ...
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topics on online barbie, text and the acids. artifacts and file of the creative patients. items of an International Symposium( Zakynthos, December 1997).
Generous supporters have donated more than 60 Barbie and Sindy dolls.. Nicky and Jo gave us the toys along with a selection of accessories.. They were from a collection built up over many years and are in immaculate condition.. The dolls have been listed on our eBay store.. Money generated from this act of kindness is very much appreciated.. ...
I think Barbie Dreamhouse Party might be the most sarcastic, darkly satirical game Ive ever played. And Im not even sure if Im joking at this point. When a
Bacillus megaterium: bacteria endofítica de callos de Ilex paraguariensis con actividad de promoción del crecimiento Renata Lúcia Grunennvaldt¹, Juliana Degenhardt-Goldbach², Jéssica de Cássia Tomasi¹, Germana Davila Dos Santos³, Vânia Aparecida Vicente³, Cícero Deschamps¹ Article Eritrea Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. Maldives Bacillus subtilis colonizes plant roots and attacks soil-borne pathogens directly. 2013), and antioxidant. Dominican Republic 1. Zimbabwe. Slovenia Malaysia Increased activity of defense-related enzymes, i.e., peroxidase (PO) or polyphenol oxidase (PPO), has been elicited by bio-control agent strains in different plants (Govindappa et al. United Arab Emirates Plants were harvested 6 months after planting, and roots were washed free from adhering soil. The activities of both peroxidase (PO) and polyphenol oxidase (PPO) showed detectable ...
LB and LA Media Spanish TranslationREVIEWERSThis resource was peer-reviewed at ASM Conference for Undergraduate Education 2006.Participating reviewers: Cynthia KelerDelaware Valley College, Doylestown, PAJames KnappHolyoke Community College, Holyoke, MADonald LehmanUniversity of Delaware, Newark, DEElise R. SullivanUniversity of New Hampshire, Durham, NHPenelope WorthingtonCreighton University, Omaha, NE Anne E. ZayaitzKutztown University of Pennsylvania, Kutztown, PA
Copyright © 2004-2018 Florida Center for Instructional Technology. ClipArt ETC is a part of the Educational Technology Clearinghouse and is produced by the Florida Center for Instructional Technology, College of Education, University of South Florida. ...
Copyright © 2004-2018 Florida Center for Instructional Technology. ClipArt ETC is a part of the Educational Technology Clearinghouse and is produced by the Florida Center for Instructional Technology, College of Education, University of South Florida. ...
TY - JOUR. T1 - Biochemical studies of bacterial sporulation and germination. XI. Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium.. AU - Deutscher, M. P.. AU - Chambon, P.. AU - Konberg, A.. PY - 1968/10/10. Y1 - 1968/10/10. UR - http://www.scopus.com/inward/record.url?scp=0014409890&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0014409890&partnerID=8YFLogxK. M3 - Article. C2 - 4971349. AN - SCOPUS:0014409890. VL - 243. SP - 5117. EP - 5125. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 19. ER - ...
casSAR Dugability of P52969 | Su-2 | Small, acid-soluble spore protein 2 - Also known as SAS2_SPOUR, Su-2. SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spores high resistance to UV light.
A 3-kb region, located downstream of the Lactobacillus brevis xylA gene (encoding D-xylose isomerase), was cloned in Escherichia coli TG1. The sequence revealed two open reading frames which could code for the D-xylulose kinase gene (xylB) and another gene (xylT) encoding a protein of 457 amino acids with significant similarity to the D-xylose-H+ symporters of E. coli, XylE (57%), and Bacillus megaterium, XylT (58%), to the D-xylose-Na+ symporter of Tetragenococcus halophila, XylE (57%), and to the L-arabinose-H+ symporter of E. coli, AraE (60%). The L. brevis xylABT genes showed an arrangement similar to that of the B. megaterium xylABT operon and the T. halophila xylABE operon. Southern hybridization performed with the Lactobacillus pentosus xylR gene (encoding the D-xylose repressor protein) as a probe revealed the existence of a xylR homologue in L. brevis which is not located with the xyABT locus. The existence of a functional XylR was further suggested by the presence of xylO sequences ...
A study was conducted to optically determine the viability of the Gram +, endospore-forming bacterial genera Bacillus using a hyperspectral reflectance spectrometer. Endospores are a dormant, differentiated cellular capsule form taken by select bacteria to ensure survival when environmental conditions become unfavorable. Two species of Bacillus were used for this study, Bacillus megaterium and Bacillus subtilis. These endospores were killed using a chemical treatment of sodium hypochlorite or a physical treatment of heat and pressure in an autoclave. The treated samples along with viable samples were lyophilized to form a powder. A reflectance spectrometer measuring 350 nm to 2500 nm (UV to shortwave infrared) was used to collect optical data on bulk powders. The resulting spectra were analyzed using several different methods, including integration and a Support Vector Machine (SVM) to obtain optimal separability of viable and nonviable endospores. Results of this study demonstrated the
The standard technology of seed processing uses mainly chemical products. Recent researches showed that toxic materials from chemical fertilizers can be harmful to humans, animals and the environment. Currently the attention of researches is shifting away from chemical fertlizers and toward alternative that consumers perceive to be natural, Plant Growth Promoting bacteria (PGP). PGP bacteria could be a way to reduce chemical fertilizer doses. This was the reason to test the ability of Bacillus megaterium, Azotobacter chroococcum to produce hormone auxin (IAA). Bacterial strains were identified by PCR amplification and sequencing of the 16S rRNA gene. Indole-3-acetic acid (IAA) was detected and quantified by MRM experiment. This study conducted that maize seed inoculation with IAA from species mentioned above showed positive effects. They had statistically significantly higher root and steam height compared to control seedlings. Bacterial strains tested in this study may be recommended ...as PGP ...
For example, if you are taking it once a week to prevent malaria, it is best to take it on the same day of each week. After chloroquine was allowed into the market, it has been used to effectively cure malaria. Thus, chloroquine modulates the tumour response to anticancer drugs (Figure 4 ). Chloroquine has activity against the blood stages of P. They were banned then. 2 Chloroquine inhibits glycosylation of the cellular angiotensin-converting enzyme 2 (ACE2) receptor, which may interfere with binding of SARS-CoV to. Chloroquine is able to accumulate at very high concentrations within the acid compartments of the parasite. The. Cited by: 3 Publish Year: 2020 Author: Eva Schrezenmeier, Thomas Dörner People also ask How does chloroquine work to treat malaria? It was shown that FIG. falciparum [ 1 ] Chloroquine is a drug has been used since World War II for treating and suppressing malaria. 1. The drug chloroquine is bactericidal for Bacillus megaterium ; it inhibits DNA and RNA biosynthesis and ...
Common Names of Plant Diseases...R. Jay Stipes, primary collator (last update 3/8/93) BACTERIAL DISEASES Bacterial wetwood Enterobacter nimipressuralis (Carter) Brenner et al. = Erwinia nimipressuralis Carter Bacillus megaterium de Bary Pseudomonas fluorescens Migula Discoloration (xylem) Bacteria Elm leaf scorch...
During the course of an investigation of the bacterial flora of certain garden soils in the vicinity of the Botanical Laboratory of Cambridge University, I succeeded, with the assistance of Professor Marshall Ward, an isolating a form which proved to be of rather exceptional interest on account of its large size and its marked predilection for acid saccharine culture-media. The method pursued consisted in taking samples of soil, with the usual precautions against contamination, at a depth of about an inch below the surface, and using portions of these, shaken up with distilled water, for the inoculation of gelatine cultures in Petri-dishes, either directly or after preliminary heating to almost the boiling point. The latter means served to prevent the growth of moulds, while it became apparent early in the course of the investigation that the use of certain acid media served very effectually to isolate the organism in question from other bacteria. ...
Our gene of the month is Ken and Barbie - yes, named after the dolls. Like their plastic childhood counterparts, male and female flies with a fault in the gene have no external genitals - their naughty bits start to develop but get stuck inside the body.The Ken and Barbie gene itself encodes the instructions to make a transcriptional repressor - a protein that prevents genes
22 Eccentric Barbie Doll Designs - The iconic blonde-haired, long-limbed doll named Barbie has been around for decades and the idol of little girls all over the globe, however, this ...
Human Barbie Valeria Lukyanova was known for her slim frame and dedication to looking like the doll, but shes now sporting a more ripped physique thanks to the gym
Wi-fi spy Barbie records childrens conversations - and could be a target for hackers, say experts Jeff Parsons www.mirror.co.uk 17 February 2015 Toymaker Mattel has announced plans to create a connected Barbie doll capable of having internet-based conversations with children Hello Barbie is the latest incarnation of the doll - and will record what your…
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Bacillus brevis • Bacillus Cereus • Bacillus megateriumBacillus subtilis • Burkholderia cepacia • Campylobacter jejuni • ...
Taku A, Stuckey M, Fan DP (1982). "Purification of the peptidoglycan transglycosylase of Bacillus megaterium". J. Biol. Chem. ...
ISBN 978-0-199-73438-2. Woese, C. R. (1960). "Phage induction in germinating spores of Bacillus megaterium". Radiation Research ...
Bacillus megaterium strain AIMST Nre8 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus megaterium strain AIMST Nse1 16S ... Bacillus megaterium strain AIMST Nae5 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus megaterium strain AIMST Ngme6 ... Bacillus megaterium strain AIMST Ngme9 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus megaterium strain AIMST Ngse10 ... Bacillus megaterium strain AIMST Nse1 (N. sanguinea; Selangor, Malaysia; leaf tissue) Bacillus megaterium strain AIMST Nse2 (N ...
Sundharadas G, Gilvarg C (1967). "Biosynthesis of alpha,epsilon-diaminopimelic acid in Bacillus megaterium". J. Biol. Chem. 242 ... 6-diaminopimelic acid by a diaminopimelate-requiring mutant of Bacillus megaterium". J. Gen. Microbiol. 115: 95-100. doi: ... Bartlett ATM; White PJ (1985). "Species of Bacillus that make a vegetative peptidoglycan containing lysine lack diaminopimelate ...
Omura H, Wieser M, Nagasawa T (April 1998). "Pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910, an organic- ... Wieser, M.; Yoshida, T.; Nagasawa, T. (1998). "Microbial synthesis of pyrrole-2-carboxylate by Bacillus megaterium PYR2910". ... "Carbon dioxide fixation by reversible pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910". European Journal ...
Many of the steps beyond uroporphyrinogen III in anaerobic organisms such as Bacillus megaterium involve chemically similar but ... Bacillus megaterium, and Propionibacterium freudenreichii subsp. shermanii. Either pathway can be divided into two parts: ... Bacillus, Clostridium, Corynebacterium, Flavobacterium, Lactobacillus, Micromonospora, Mycobacterium, Nocardia, Proteus, ...
These roots form interactions with rhizosphere bacteria such as Bacillus megaterium. A. thaliana can complete its entire ... "Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent ...
Pauly HE, Pfleiderer G (1976). "D-Glucose dehydrogenase from Bacillus megaterium M 1286: purification, properties and structure ...
Huang C; Chen, MW; Hsieh, JL; Lin, WH; Chen, PC; Chien, LF (2006). "Expression of mercuric reductase from Bacillus megaterium ...
H2O The enzyme from Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta. Berg A, Ingelman- ... "Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium". The Journal of ... "Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368". Biochimica et ...
Bacillus megaterium Bacillus subtilis One biological system that might be of key importance in future development of ... Bacillus megaterium spores and suitable dried nutrients are mixed and applied to steel‐reinforced concrete. When the concrete ... Bacillus subtilis has already been shown to respond to its environment, by changing the production of its ECM. It uses the ... Glutamate in Bacillus subtilis". Journal of Bacteriology. 196 (4): 740-753. doi:10.1128/JB.01217-13. PMC 3911173. PMID 24296669 ...
P450 BM3 (also known as CYP102A1) is a cytochrome P450 enzyme isolated from Bacillus megaterium. BM3 has been extensively ...
Ala This enzyme participates in spore germination in Bacillus megaterium. Ponnuraj K, Rowland S, Nessi C, Setlow P, Jedrzejas ... "Crystal structure of a novel germination protease from spores of Bacillus megaterium: structural arrangement and zymogen ...
In 1926, French microbiologist Maurice Lemoigne chemically identified the polymer after extracting it from Bacillus megaterium ...
"Activities and regulation of the enzymes of lysine biosynthesis in a lysine-excreting strain of Bacillus megaterium". J. Gen. ...
Patil AG, K PK, Mulimani VH, Veeranagouda Y, Lee K (November 2010). "alpha-Galactosidase from Bacillus megaterium VHM1 and its ...
Bacteria include Bacillus firmus, B. licheniformis, B. megaterium, B. pumilus, and Streptomyces and fungus includes ...
... and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium". Applied and ... Sporosarcina ureae is a type of bacteria of the genus Sporosarcina, and is closely related to the genus Bacillus. S. ureae is ... ureae is closely related to other spore-forming organisms of the genus Bacillus, an observation first noted by Beijerinck in ...
DasGupta H, Fan DP (July 1979). "Purification and characterization of a carboxypeptidase-transpeptidase of Bacillus megaterium ...
"Optimization of culture conditions for production of B-galactosidase by bacillus megaterium NM56 isolated from raw milk" (PDF ... "Optimization of Culture Conditions for Production of B-Galactosidase by Bacillus Megaterium NM56 Isolated from Raw Milk" (PDF ... "Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants ...
... from the soil bacterium Bacillus megaterium catalyzes the NADPH-dependent hydroxylation of several long-chain fatty acids at ... of a catalytically self-sufficient 119,000-dalton cytochrome P-450 monooxygenase induced by barbiturates in Bacillus megaterium ...
... is a Prokaryote Cytochrome P450 enzyme originally from Bacillus megaterium, catalyzes the hydroxylation of ... of a catalytically self-sufficient 119,000-dalton cytochrome P-450 monooxygenase induced by barbiturates in Bacillus megaterium ...
Bacillus megaterium has been shown to cause about an 80% decrease in disease development in the trials done so far. ... A benefit to using pseudomonads or bacillus is that they are not harmed by most fungicides, so they can be used in combination ...
"Metabolism of 6-O-acetyl-D-glucopyranose and other monoacetyl-sugars by strains of Bacillus megaterium and other soil organisms ...
The agar was streaked with different bacteria such as Bacillus megaterium, Serratia marcescens, Pseudomonas fluorescens, ... The strains with B.megaterium, S.marcescens, and P.fluorescens sorocarps did develop, but lesser and later than normal. In the ...
... in anaerobic bacteria such as Salmonella typhimurium and Bacillus megaterium. Cobalamin biosynthesis Kajiwara Y, Santander PJ, ...
... reductive dehalogenase was heterologously expressed in a soluble and active form and purified using Bacillus megaterium. In ...
... in bacteria such as Salmonella typhimurium and Bacillus megaterium. It has also been identified as the enzyme which inserts ...
The GerN and GrmA proteins of Bacillus cereus and Bacillus megaterium, respectively, are spore germination proteins that can ... "Cloning and sequencing of the spore germination gene of Bacillus megaterium ATCC 12872: similarities to the NaH-antiporter gene ... Bakker EP, Borchard A, Michels M, Altendorf K, Siebers A (September 1987). "High-affinity potassium uptake system in Bacillus ... Southworth TW, Guffanti AA, Moir A, Krulwich TA (October 2001). "GerN, an endospore germination protein of Bacillus cereus, is ...
Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic spore forming bacterium found in widely diverse habitats. With ... The species was described by de Bary in 1884, who called it Bacillus megaterium, but did not give an etymology. However, some ... Bacillus megaterium - from simple soil bacterium to industrial protein production host. Appl Microbial Biotechnol 76:957-967 ( ... John-Brooks, R. (1951). "Opinion 1. The Correct Spelling of the Specific Epithet in the Species Name Bacillus Megaterium De ...
Bacillus megaterium Domain: Prokaryote Optimal Growth Medium: Nutrient Agar Optimal Growth Temperature: 30° C Package: Tube ... Biosafety Level: 1 Gram Stain: Gram-Positive Shape: Bacillus (rod-shaped) ... Bacillus megaterium, Living, Tube. Item # 154900 Bacillus megaterium, Living, Tube is rated 5.0 out of 5 by 1. ... Genus and Species: Bacillus megaterium. Domain: Prokaryote. Optimal Growth Medium: Nutrient Agar Optimal Growth Temperature: 30 ...
Bacillus megaterium ATCC ® 14581™ Designation: TypeStrain=True Application: VITEK 2 BCL Comprehensive QC Set Quality control ... Nucleotide (GenBank) : X59070 B.megaterium spoOH gene for sigma H factor. Nucleotide (GenBank) : X16610 Bacillus megaterium ... Bacillus megaterium beta-galactosidase (mbgA) gene, complete cds. Nucleotide (GenBank) : J04832 B.megaterium cytochrome P-450: ... Bacillus megaterium de Bary Strain Designations [BCRC 10608, CCM 2007, CCUG 1817, CIP 66.20, DSM 32, HAMBI 2018, IAM 13418, JCM ...
Bacillus megaterium spores have a characteristic polar knob and equatorial ridge, or groove. During germination, the spore case ...
Bacillus megaterium ATCC ® 14581-MINI-PACK™ Designation: TypeStrain=True Application: ATCC ® 14581-MINI-PACK™ consists of 6 ... Nucleotide (GenBank) : X59070 B.megaterium spoOH gene for sigma H factor. Nucleotide (GenBank) : X16610 Bacillus megaterium ... Bacillus megaterium beta-galactosidase (mbgA) gene, complete cds. Nucleotide (GenBank) : J04832 B.megaterium cytochrome P-450: ... Bacillus megaterium de Bary (ATCC® 14581-MINI-PACK™) Strain Designations: [BCRC 10608, CCM 2007, CCUG 1817, CIP 66.20, DSM 32, ...
Bacillus megaterium bacterial bulture for microbiology laboratory studies is large, easily observed, spore-forming variably ... Bacillus megaterium. Large, easily observed, variably motile rods found in soil and water. Spore forming. Incubation ... Bacillus megaterium bacterial bulture for microbiology laboratory studies is large, easily observed, spore-forming variably ...
Copyright © 2004-2018 Florida Center for Instructional Technology. ClipArt ETC is a part of the Educational Technology Clearinghouse and is produced by the Florida Center for Instructional Technology, College of Education, University of South Florida. ...
"A pair of bacilli actively growing and dividing." - The Encyclopedia Britannica, 1910 ...
MoBiTec provides a wide range of useful vectors for the Bacillus megaterium system, including a vector with two ribosome ... vectors for Bacillus megaterium - MoBiTec provides a wide range of useful vectors for the Bacillus megaterium system, including ... B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli ... Simultaneous Heterologous Gene Expression in Bacillus Megaterium: Vector p3STOP1623-2RBShp. Optimized high performance (hp) ...
sp,Q60136,PAC_BACME Penicillin G acylase OS=Bacillus megaterium OX=1404 GN=pac PE=1 SV=1 ... Bacillus megaterium. ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and ...
Sulfur metabolism - Bacillus megaterium DSM 319 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Biotin metabolism - Bacillus megaterium WSH-002 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
... we identified strain Q3 as Bacillus megaterium. We investigated the effects of temperature, pH, inoculation size, and initial ... Bacillus Is the Subject Area "Bacillus" applicable to this article? Yes. No. ...
Bacillus megaterium de Bary, 1884. Taxonomic Serial No.: 959826 (Download Help) Bacillus megaterium TSN 959826 ...
Bacillus megaterium. Bacillus sp. Leaf75. Bacillus megaterium (strain ATCC 12872 / QMB1551). Bacillus megaterium Q3. Bacillus ... Bacillus sp. FJAT-21351. Bacillus megaterium (strain WSH-002). Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / ... Bacillus licheniformis. Bacillus sp. LM 4-2. Bacillus atrophaeus. Bacillus intestinalis. Bacillus cereus. And more. 583. ... Bacillus subtilis (strain 168). Bacillus subtilis subsp. subtilis. Bacillus subtilis. Bacillus subtilis BEST7613. Bacillus ...
During the past 2 decades, Bacillus megaterium has been systematically developed for the gram-per-liter scale production of ... Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity.. ... Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity ... Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity ...
Bacillus megaterium IAM 1030 (Bacillus sp. JCM 20016) possesses four d-glucose 1-dehydrogenase isozymes (BmGlcDH-I, -II, -III ... Bacillus megaterium IAM 1030 (Bacillus sp. JCM 20016) possesses four d-glucose 1-dehydrogenase isozymes (BmGlcDH-I, -II, -III ... Crystal structure of Bacillus megaterium glucose dehydrogenase 4 in ligand-free form. *DOI: 10.2210/pdb3AUS/pdb ... Structure-guided mutagenesis for the improvement of substrate specificity of Bacillus megaterium glucose 1-dehydrogenase IV. ...
Crystal structure of dual function transcriptional regulator MerR from Bacillus megaterium MB1. *DOI: 10.2210/pdb4UA2/pdb ... bound MerR from Bacillus megaterium, corresponding respectively to the repressor and activator conformation of MerR. To our ... Bacillus megaterium. Mutation(s): 0 Gene Names: merR, merR1. Find proteins for Q799U3 (Bacillus megaterium) ...
Molecular Sieving by the Bacillus megaterium Cell Wall and Protoplast Message Subject (Your Name) has forwarded a page to you ... Molecular Sieving by the Bacillus megaterium Cell Wall and Protoplast. Rene Scherrer, Philipp Gerhardt ... Passive permeabilities of the cell wall and protoplast of Bacillus megaterium strain KM were characterized by use of 50 ...
1993) The genetic map of Bacillus megaterium. in Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, ... 1984) Bacillus megaterium spore protein C-3: nucleotide sequence of its gene and the amino acid sequence at its spore cleavage ... Polyhydroxyalkanoate Inclusion Body-Associated Proteins and Coding Region in Bacillus megaterium Gabriel J. McCool, Maura C. ... Polyhydroxyalkanoate Inclusion Body-Associated Proteins and Coding Region in Bacillus megaterium Gabriel J. McCool, Maura C. ...
ALPHA-AMYLASE FROM BACILLUS MEGATERIUM EXPRESSED IN BACILLUS SUBTILIS 1. EXPLANATION Enzymes used for the hydrolysis of starch ... ZEMAN, N.W. (1990). Additional safety information on the amylase of Bacillus megaterium derived from Bacillus subtilis. ... See Also: Toxicological Abbreviations alpha-AMYLASE FROM BACILLUS MEGATERIUM EXPRESSED IN BACILLUS SUBTILIS (JECFA Evaluation) ... The authors concluded that the NOEL for Bacillus megaterium amylase in rats exposed in utero and for 13 weeks after weaning is ...
Energy Conservation in Bacillus megaterium. ANDREW J. DOWNS, COLIN W. JONES. Biochemical Society Transactions Jun 01, 1974, 2 ( ... Energy Conservation in Bacillus megaterium Message Subject (Your Name) has forwarded a page to you from Biochemical Society ...
The Gram-positive aerobe Bacillus megaterium has previously been used for the commercial production of cobalamin and has a ... By studying the purified B. megaterium CbiH60 enzyme, an efficient method was developed to allow the generation of the ring- ... megaterium DSM319. One of the major bottlenecks in the anaerobic pathway is the ring contraction step, where only limited ... megaterium, by incubating combinations of purified enzymes with cobalt-factor IV and the appropriate cofactors (SAM, NADH). ...
We investigated the central carbon metabolism of Bacillus megaterium QM B1551 cells using various carbon substrates, long-term ... It was shown in the same experiment that B. megaterium QM B1551 did not use the Entner-Doudoroff (ED) pathway for glucose (Gluc ... Finally, it was observed that B. megaterium QM B1551 had an early preference for glutamate catabolism in the TCA cycle, ... We determined that gluconate can be incorporated into the metabolic network structure of B. megaterium QM B1551, particularly ...
... such as Bacillus subtilis [5], Bacillus firmus [32], Bacillus licheniformis [33], Bacillus mucilaginosus [2], Proteus mirabilis ... from Bacillus megaterium TF10 exhibit a high flocculation activity [36]. One report of a Bacillus megaterium strain producing a ... The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L−1 of glucose and 0.5 g L−1 of beef extract at ... The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L−1 of glucose and 0.5 g L−1 of beef extract at ...
Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response ... Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response ...
Expression in Escherichia coli of a His-tagged form of Bacillus megaterium PBGD permitted the crystallization and preliminary X ... The Gram-positive bacterium Bacillus megaterium is of great industrial interest since it has many commercial applications in ... The B. megaterium PBGD gene was cloned into the NdeI and BamHI restriction sites of the expression vector pET14b using standard ... as the search model was successful and refinement of the B. megaterium PBGD structure is currently in progress using this high- ...
Bacillus megaterium CGTase produces those three types of CDs, however, β-CD should prevail. Although waxy corn starch (CS) is ... Bacillus megaterium CGTase produces those three types of CDs, however, β-CD should prevail. Although waxy corn starch (CS) is ... B. megaterium produced a 66 kDa CGTase (Topt=50°C; pHopt=8.0), from the early exponential growth phase which lasted 36 h. The ... B. megaterium produced a 66 kDa CGTase (Topt=50°C; pHopt=8.0), from the early exponential growth phase which lasted 36 h. The ...
I feel like the first one is bacillus subtillis and the second one is bacillus megaterium As they are both positive in the ... Identification beteeen bacillus subtillis and bacillus megaterium?. About microscopic forms of life, including Bacteria, Archea ...
Shaking-flask production of recombinant proteins using Bacillus megaterium.The B. megaterium strain MS941, an nprM deletion ... such as Bacillus megaterium, have gained growing interest (26, 33). Similar to E. coli, B. megaterium is able to utilize a wide ... The Bacillus megaterium protein production system based on the inducible promoter of the xyl operon (PxylA) was systematically ... High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium Simon Stammen, Britta Katrin Müller, Claudia ...
  • In the 1960s, prior to the utilization of Bacillus subtilis for this purpose, B. megaterium was the main model organism among Gram-positive bacteria for intensive studies on biochemistry, sporulation and bacteriophages. (wikipedia.org)
  • Bacterial (Bacillus subtilis) alpha- amylase has been in common use to control the viscosity of chocolate syrup since 1929 and in the brewing industry since 1936. (inchem.org)
  • This alpha-amylase from Bacillus subtilis B1-109 (ATCC 39,701) containing plasmid pCPC 800 [amylase gene from B. megaterium (NCIB 11568)] and regulating sequences from alpha-amylase of B. stearothermophilus (see Appendix 2) has not been previously evaluated by the Committee. (inchem.org)
  • In comparison to industrially employed Bacillus subtilis strains, B. megaterium strains have the advantage of highly stable, freely replicating plasmids and the lack of alkaline proteases ( 30 ). (asm.org)
  • Here, we provide evidence that pBM600-encoded orthologues of the Bacillus subtilis CotW and CotX proteins, which form the crust layer in spores of that species, are structural components of the Bacillus megaterium QM B1551 spore exosporium. (asm.org)
  • The Gram-positive bacteria Bacillus brevis [ 16 , 17 ] and Bacillus subtilis [ 18 , 19 ] have already been succesfully used for the production of antibody fragments. (biomedcentral.com)
  • In contrast to B. subtilis , B. megaterium does not produce alkaline proteases. (biomedcentral.com)
  • grown in vitro and incubated anaerobically in salts solution, released into the medium protein containing hexokinase and an enzyme which lysed Bacillus megaterium, B. subtilis and Micrococcus lysodeikticus. (microbiologyresearch.org)
  • Bacillus megaterium and B. subtilis are bacteria that are useful for plants. (living-soils.com)
  • Here's a brief introduction to two key beneficial plant bacteria, Bacillus subtilis and Bacillus megaterium . (living-soils.com)
  • Bacillus subtilis, or B. sub , is a key beneficial plant bacteria. (living-soils.com)
  • In addition to some of the benefits conferred by B. subtilis, B. megaterium is known to be a phosphate solubilizing bacteria (PSB). (living-soils.com)
  • Maldives Bacillus subtilis colonizes plant roots and attacks soil-borne pathogens directly. (saundhaus.com)
  • Germany Mayotte with strains of Bacillus megaterium and Bacillus muciaraglaginous together with AMF was associated with improved nutritional assimilation of plant total N, P, and K. Application of PGPR resulted in a significant increase in N, P Palestinian Territory B. subtilis, B. megaterium, and B. pumilus have shown nematicidal activity against M. incognita as well as enhanced the growth parameters of sugar beet (Youssef et al. (saundhaus.com)
  • With a cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria. (wikipedia.org)
  • In this work it was carried the production of PHA poly(3-hydroxybutyrate) (P3HB) out in submerse cultures of bacteria Bacillus megaterium in the mineral medium, using sucrose as carbon source and ammonium sulphate as nitrogen source. (ufrgs.br)
  • Another key beneficial plant bacteria is Bacillus megaterium. (living-soils.com)
  • Bacillus megaterium: bacteria endofítica de callos de Ilex paraguariensis con actividad de promoción del crecimiento Renata Lúcia Grunennvaldt¹, Juliana Degenhardt-Goldbach², Jéssica de Cássia Tomasi¹, Germana Davila Dos Santos³, Vânia Aparecida Vicente³, Cícero Deschamps¹ Article Eritrea Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. (saundhaus.com)
  • Spores of Bacillus bacteria are regularly consumed by animals and humans inadvertently through their foods and drinks and in some fermented foods. (saundhaus.com)
  • Bacillus thuringiensis (or BT) is a living microorganism belonging to the family of bacteria. (saundhaus.com)
  • Tierra Overseas is manufacturer and exporter of high quality Bacillus Megaterium (Phosphate Solubilising Bacteria) in India. (tierraoverseas.com)
  • Bacillus megaterium is a non-pathogenic, spore-forming bacteria that is found in soil, but recently a strain of B. megaterium was reported to cause a mild case of diarrhea in an infant. (niu.edu)
  • Bacillus megaterium) Bacteria. (gettyimages.dk)
  • Bacillus megaterium is a non pathogenic soil dwelling bacteria. (gettyimages.dk)
  • Bacillus megaterium ATCC 19213 secretes a cell division-initiating "schizokinen" (SK) which accumulates during its culture cycle to a concentration inversely proportional to the iron added to a sucrose-mineral salts medium. (docphin.com)
  • Inferência de variáveis do processo de produção de penicilina G acilase por Bacillus megaterium ATCC-14945. (ufscar.br)
  • On-line measurement and monitoring of cultivations of Bacillus megaterium ATCC 14945 in a agitated and aerated bioreactor allowed the acquisition of variables such as mol fraction of CO2 and O2 in the exhaust gases, pH, dissolved oxygen. (ufscar.br)
  • In our study, the keratinase gene from the chromosomal DNA of Bacillus licheniformis ATCC®53757 was PCR amplified and subsequently cloned into Bacillus megaterium expression vector, pHIS1525.SPlipA. (diva-portal.org)
  • Bacillus megaterium ATCC®14945 strain was transformed with the recombinant plasmid, pKERHIS1525.SPlipA. (diva-portal.org)
  • B. megaterium has some phenotypic and phylogenetic similarities with pathogens B. anthracis and B. cereus, although itself being relatively harmless. (wikipedia.org)
  • The outermost layer, or exosporium, in Bacillus megaterium QM B1551 spores is of interest, as it is morphologically distinct from the exosporia of spores of the pathogenic Bacillus cereus family. (asm.org)
  • In this work, we provide evidence that structurally important protein constituents of the Bacillus megaterium exosporium are different from those in the Bacillus cereus family. (asm.org)
  • The best studied of these exosporial structures belong to spores of the Bacillus cereus family, which includes Bacillus anthracis , the causative agent of anthrax. (asm.org)
  • The pPconst1326 carries the oriU and repU gene from Bacillus cereus for replication in Bacillus sp. (mobitec.com)
  • Bacillus cereus is a related species and a known gastroenteric pathogen. (niu.edu)
  • The pathogenic strain of B. megaterium, (CHI), was tested by PCR and hybridization, to see if it picked up either of these two factors that cause pathogenicity in B. cereus by plasmid exchange. (niu.edu)
  • WILKINSON, J. F. Poly +hyroxybutyrate Metabolism in Washed Suspensions of Bacillus cereus and Bacillus megaterium. (blucher.com.br)
  • Several genes, (termed chi for 9,obinamide biosynthesis) from the cobalamin biosynthetic pathway have been cloned and overexpressed individually within the host B. megaterium DSM319. (bl.uk)
  • To generate the pPconst1326 constitutive vector the promoter of pyruvate dehydrogenase operon (pdhABCD, bmd_1326-1329 ) was isolated from B. megaterium DSM319 and cloned into an E. coli/ Bacillus shuttle vector. (mobitec.com)
  • Here, we report on the expression of the penicillin G amidase gene ( pga ) isolated from B. megaterium ATCC14945 in derivatives of B. megaterium DSM319. (biomedcentral.com)
  • Here, we present a genome-scale metabolic model for B. megaterium DSM319, iJA1121, which is a result of a metabolic network reconciliation process. (chalmers.se)
  • According to the blast analysis of 16S rDNA (1434 bp), the strain showed 99% identity with Bacillus megaterium DSM319. (cu.edu.tr)
  • Properties of a Bacillus megaterium DSM319 extracellular protease which are related to its application for depilating hides were investigated. (permi.or.id)
  • Nitrogen fixation has been demonstrated in some strains of B. megaterium. (wikipedia.org)
  • The method of described in can be used to isolate strains of B. megaterium from the soil. (wikipedia.org)
  • Unlike other bacilli strains, proteolytic degradation by external alkaline proteases is avoided. (pr.com)
  • The enzyme preparation derived from these various Bacillus strains is usually added directly to the food to be processed and then removed from the final product by filtration. (inchem.org)
  • Recently, a set of free replication vectors and genetically optimized B. megaterium strains for the intra- and extracellular production of affinity tagged recombinant proteins were developed. (biomedcentral.com)
  • SUMMARY: The surface structures and serological typing of 54 strains of Bacillus megateruim were examined and compared with strain M. Morphologically the strains could be divided into 2 groups on the basis of cell size, fat content and susceptibility to enzymes. (microbiologyresearch.org)
  • Agglutination reactions confirmed the heterogeneity of the polysaccharides in the cell wall and capsules of B. megaterium and demonstrated a further relationship between 3 of the strains, probably due to the flagellar antigen. (microbiologyresearch.org)
  • In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. (bvsalud.org)
  • Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. (bvsalud.org)
  • During the past 2 decades, Bacillus megaterium has been systematically developed for the gram-per-liter scale production of recombinant proteins. (nih.gov)
  • This work presents the results of a study of PHA inclusion body-associated proteins and an analysis of their coding region in Bacillus megaterium 11561. (asm.org)
  • We now present the results of a study of PHA inclusion body-associated proteins from B. megaterium and the cloning and analysis of their coding region. (asm.org)
  • This thesis demonstrates the use of functionalized superparamagnetic iron oxide nanoparticles for the purification of recombinant histidine-tagged proteins directly from a growing culture of the gram-positive bacterium Bacillus megaterium. (cuvillier.de)
  • Recently, the Gram-positive bacterium Bacillus megaterium was shown to efficiently secrete recombinant proteins into the growth medium. (biomedcentral.com)
  • Interestingly, these parameters were very different to the optimal conditions for the expression of other proteins in B. megaterium . (biomedcentral.com)
  • Moreover, sequenced phages encode proteins associated with Bacillus pathogenesis. (peerj.com)
  • During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. (biomedcentral.com)
  • B. megaterium has often been used in the laboratory, and is used as an industrial organism that is able to produce a variety of proteins and sources of bioremediation. (saundhaus.com)
  • During the last decade Bacillus megaterium was systemically developed for the gram per liter production of recombinant proteins. (tu-bs.de)
  • Bacillus megaterium is a microorganism widely used in industrial biotechnology for production of enzymes and recombinant proteins, as well as in bioleaching processes. (chalmers.se)
  • Over the course of various cloning and expression experiments of similar proteins, it was found that Bacillus megaterium could be a susceptible host cell for keratinase production. (diva-portal.org)
  • In order to examine radiation -induced proteins in an extremely radio -resistant bacterium, it became possible to perform comparative proteomic analysis on radio -resistance Bacillus megaterium WHO as a wild-type strain for the first time . (bvsalud.org)
  • Variation in cellular proteins profiles of the Bacillus megaterium WHO after 5 KGy gamma -irradiation were analyzed by two-dimensional poly acryl amide gel electrophoresis and silver staining . (bvsalud.org)
  • Our results provide initial insights into the mechanism of cellular heterogeneity during plasmid-based recombinant protein production in a Bacillus species. (nih.gov)
  • The most commonly employed gene expression system is based on the xylose-inducible P xylA promoter of the B. megaterium xyl operon, usually localized on a freely replicating plasmid. (asm.org)
  • Did Bacillus megaterium pick up plasmid virulence genes? (niu.edu)
  • Phenotypic instability of plasmid in Bacillus megaterium, originated from segregation during cell growth. (kaist.ac.kr)
  • Several of these bioactive compounds are cyclic lipopeptides, belonging to the surfactin, iturin and fengycin lipopeptide families, which are also produced by several other Bacillus species. (wikipedia.org)
  • Phylogenetically, based on 16S rRNA, B. megaterium is strongly linked with B. flexus, the latter distinguished from B. megaterium a century ago, but only recently confirmed as a different species. (wikipedia.org)
  • The species was described by de Bary in 1884, who called it Bacillus megaterium, but did not give an etymology. (wikipedia.org)
  • Expression in Escherichia coli of a His-tagged form of Bacillus megaterium PBGD permitted the crystallization and preliminary X-ray analysis of the enzyme from this species at high resolution. (iucr.org)
  • The exosporium of Bacillus megaterium QM B1551 spores is morphologically distinct from exosporia observed for the spores of many other species. (asm.org)
  • Based on multiple-genome alignments and available genome-scale metabolic models for other Bacillus species, we constructed a draft network using an automated approach followed by manual curation. (chalmers.se)
  • Schizokinen also overcame ferrimycin A inhibition of three Bacillus species. (docphin.com)
  • Here we report the presence of numerous natural variants of P450 BM3 (CYP102A1) within a species of Bacillus megaterium . (springeropen.com)
  • In addition, this is the first study exploring a Bacillus species isolated from an alkaline environment adept in utilizing 2,2-DCP as a sole source of carbon. (iium.edu.my)
  • 2.2.2 Short term studies 2.2.2.1 Rat Groups of 5 male and 5 female rat (Fischer 344) were exposed to enzyme levels of either 0.0, 20.0, 60.0 or 100.0 U/g Bacillus megaterium for 14 days (equivalent to 0, 1, 3 or 5% liquid use product or approximately 0, 0.32, 0.99 or 1.57 g/kg b.w./day), in the diet (alpha-amylase 6000 U/g). (inchem.org)
  • 2.2.2.2 Dog Groups of 1 male and 1 female dog (Beagle dogs, 6-7 months of age) were exposed to enzyme levels of either 0.0, 20.0, 60.0 or 100.0 U/g Bacillus megaterium for 15 days (approximately 0, 0.12, 0.29 or 0.74 g/kg b.w./day) in the diet (alpha-amylase 6000 U/g). (inchem.org)
  • Groups of 4 male and 4 female dogs (Beagle dogs, 6-7 months of age) were exposed to enzyme at levels of 0, 20, 60 or 100 U/g Bacillus megaterium amylase based on 5100 units of amylase/g of test material for 13 weeks in the diet (approximately 0, 0.12, 0.32 or 0.57 g/kg b.w./day). (inchem.org)
  • By studying the purified B. megaterium CbiH60 enzyme, an efficient method was developed to allow the generation of the ring-contracted product cobalt-factor IV in high yields. (bl.uk)
  • Production of protease enzyme from fish guts using Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium. (alliedacademies.org)
  • The Utilization of Bacillus megaterium and the Release of a Lytic Enzyme by Three Epidinium spp. (microbiologyresearch.org)
  • Many microorganisms produce PGA, but Bacillus megaterium is one of the few that excretes the enzyme to the extra cellular medium, facilitating downstream operations. (ufscar.br)
  • An algorithm based on the Fuzzy logic theory was implemented aiming to determine the maximum enzyme concentration during Bacillus megaterium cultivations. (ufscar.br)
  • SDS-PAGE analysis showed that the tyrosinase enzyme from Bacillus megaterium strain M36 is about 34 kDa. (cu.edu.tr)
  • Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium. (elsevier.com)
  • Application of Fuzzy Logic in the Production of Penicillin G Acylase in Bacillus megaterium Cultivations. (ufscar.br)
  • Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study. (helmholtz-hzi.de)
  • Bacillus megaterium bacterial bulture for microbiology laboratory studies is large, easily observed, spore-forming variably motile rods found in soil and water. (flinnsci.com)
  • Buch, JK , Buch, S & Dave, PJ 1982, ' Microsome-mediated mutagenicity of aflatoxin B 1 in Bacillus megaterium SJ-6 his - ', FEMS Microbiology Letters , vol. 13, no. 4, pp. 349-352. (elsevier.com)
  • Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity. (nih.gov)
  • Previous work has demonstrated that unidentified genes carried on one of the large indigenous plasmids are required for the assembly of the Bacillus megaterium exosporium. (asm.org)
  • Bacillus megaterium QM B1551 has seven indigenous plasmids ranging from 5.5 kb to 165 kb. (niu.edu)
  • B. megaterium has been an important industrial organism for decades. (wikipedia.org)
  • This thesis reports the research on mixed cultures of a high-temperature strain of algae, Chlorella pyrenoidosa TX71105, and an organism isolated from the air and tentatively identified as a variant strain of Bacillus megaterium. (unt.edu)
  • A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. (hindawi.com)
  • In this work, we successfully separated and characterized EP from EPS of the bacterium Bacillus megaterium TF10. (jesc.ac.cn)
  • Bacillus megaterium , a bacterial antagonist that is effective against sheath blight of rice, was cultured using either nutrient broth, potato dextrose broth or a common household flavour enhancer. (nzpps.org)
  • Goettingen, Germany, May 05, 2017 --( PR.com )-- The E. coli/Bacillus megaterium shuttle vector p3STOP1623-2RBShp is the only vector worldwide suitable for simultaneous dual expression of heterologous genes in B. megaterium. (pr.com)
  • B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. (pr.com)
  • Time course of heterologous GFP production by recombinant B. megaterium and analysis of subpopulations by using flow cytometry. (nih.gov)
  • In addition to the recently described T7 RNA polymerase-dependent protein production system ( 13 ), sucrose- and xylose-inducible promoter systems were successfully applied for heterologous protein production in B. megaterium ( 4 , 22 , 23 ). (asm.org)
  • We investigated the central carbon metabolism of Bacillus megaterium QM B1551 cells using various carbon substrates, long-term isotopic enrichment experiments with 13C-labeled carbons, metabolic flux analysis (MFA), and kinetic incorporation of 13C-labeled carbons. (cornell.edu)
  • We determined that gluconate can be incorporated into the metabolic network structure of B. megaterium QM B1551, particularly when gluconate was present in the environment. (cornell.edu)
  • It was shown in the same experiment that B. megaterium QM B1551 did not use the Entner-Doudoroff (ED) pathway for glucose (Gluc) metabolism, and instead relied primarily on the Embden-Mayerhof-Parnas (EMP) and oxidative pentose phosphate (PP) pathways. (cornell.edu)
  • Finally, it was observed that B. megaterium QM B1551 had an early preference for glutamate catabolism in the TCA cycle, followed by a preference for Gluc catabolism across the metabolic network. (cornell.edu)
  • Additionally, purified recombinant CotW protein is shown to assemble at the air-water interface to form thin sheets of material, which is consistent with the idea that this protein may form a basal layer in the Bacillus megaterium QM B1551 exosporium. (asm.org)
  • Optimized high performance (hp) vectors for Bacillus megaterium - MoBiTec provides a wide range of useful vectors for the Bacillus megaterium system, including a vector with two ribosome binding sites (2RBS) for simultaneous dual expression. (pr.com)
  • Zinc release is the first quantitatively significant event detected during the triggering of Bacillus megaterium KM spore germination. (biochemj.org)
  • Furthermore, B. megaterium KU143 and P. protegens AS15 produced volatile compounds that could reduce conidial germination and germ tube elongation, as well as mycelial growth of the predominant fungi ( 4 , 5 ). (asm.org)
  • Precise understanding of its metabolism is essential for designing engineering strategies to further optimize B. megaterium for biotechnology applications. (chalmers.se)
  • We conclude that the genome-scale metabolic model iJA1121 represents a useful tool for systems analysis and furthers our understanding of the metabolism of B. megaterium. (chalmers.se)
  • To determine the optimum condition (c.q. optimum pH and optimum temperature), needed for the activity of penicillin acylase isolated from Bacillus meaterium. (mediamedika.net)
  • Neste trabalho realizou-se a produção do PHA poli(3- hidroxibutirato) (P3HB) em cultivos submersos da bactéria Bacillus megaterium em meio mineral, utilizando-se sacarose como fonte de carbono e sulfato de amônio como fonte de nitrogênio. (ufrgs.br)
  • Aplicação da lógica fuzzi na produção de penicilina G Acilase em cultivos de Bacillus megaterium. (ufscar.br)
  • In this study, we describe the biological characteristics, whole genome sequences, and annotations for two new isolates of the B. megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. (peerj.com)
  • An amylase that hydrolyzes starch into maltopentaose as a main product was found in the culture supernatant of a strain of Bacillus megaterium KSM B-404 isolated from local soil. (koreascience.or.kr)
  • Purification and Characterization of a Maltopentaose-producing Amylase from Bacillus megaterium KSM B-404. (koreascience.or.kr)
  • The kinetic and biochemical characterization of Bacillus megaterium levansucrase SacB variants Y247A, Y247W, N252A, D257A, and K373A reveal novel surface motifs remote from the sucrose binding site with distinct influence on the polysaccharide product spectrum. (helmholtz-hzi.de)
  • Counteraction of inhibitory effects of aflatoxin in Bacillus megaterium. (cftri.com)
  • To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles. (bvsalud.org)
  • In general, the genomes of B. megaterium KU143, M. testaceum KU313, and P. protegens AS15 contain genes related to biocontrol traits (i.e., siderophore, polyketide, phosphate solubilization, motility, and biofilm formation) that may be significant factors in the host colonization and protection against pathogenic infections ( 7 - 10 ). (asm.org)
  • The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale. (pr.com)
  • The Bacillus megaterium protein production system based on the inducible promoter of the xyl operon (P xylA ) was systematically optimized. (asm.org)
  • Target gene cloning in E. coli and protein production in Bacillus sp. (mobitec.com)
  • The Gram-positive aerobe Bacillus megaterium has previously been used for the commercial production of cobalamin and has a complete anaerobic pathway. (bl.uk)
  • CGTase production, mainly by Bacillus sp. (frontiersin.org)
  • Therefore, the aim of this work was evaluate the AS as carbon source for CGTase production by B. megaterium in a submerged fermentation. (frontiersin.org)
  • Due to the well-documented limitations in the application of the most prominent production host, Escherichia coli , for extracellular and high-molecular-mass protein formation, alternative production hosts, such as Bacillus megaterium , have gained growing interest ( 26 , 33 ). (asm.org)
  • Here we evaluated B. megaterium for the recombinant production of antibody fragments. (biomedcentral.com)
  • High yields of functional scFv antibody fragments can be produced and secreted into the culture medium by B. megaterium , making this production system a reasonable alternative to E. coli . (biomedcentral.com)
  • Three proteolytic organisms (Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium) were used in the production of protease and the most effective production was observed at a temperature of 45°C and pH 9. (alliedacademies.org)
  • P. fluorescens produced protease best at 3rd production of 48 and 72 hours, E. cloacae produced protease best at 3rd production at 72 hours and B. megaterium produced best at 24 hours of 3rd production. (alliedacademies.org)
  • Here, recombinant production and export of B. megaterium ATCC14945 penicillin G amidase (PGA) which is used in the reverse synthesis of β-lactam antibiotics were systematically improved. (biomedcentral.com)
  • The combination of genetic and medium optimization led to an overall 7-fold improvement of PGA production and export in B. megaterium . (biomedcentral.com)
  • In contrast, using B. megaterium to secrete homologous B. megaterium PGA directly into the growth medium should facilitate its purification and consequently decrease the downstream processing and final production costs. (biomedcentral.com)
  • In this contribution we tested directed molecular strategies for the stepwise improvement of PGA production and secretion using B. megaterium . (biomedcentral.com)
  • Kinetics of carboxymethyl cellulase (EC 3.2.1.4) production by recombinant cell, $\mbox{\underline{Bacillus}}\quad\mbox{\underline{megaterium}}$ (pCK108), was investigated in a chemically defined medium. (kaist.ac.kr)
  • The production of carboxymethyl cellulase from $\mbox{\underline{Bacillus}}\quad\mbox{\underline{megaterium}}$ (pCK108) was regulated by carbon catabolite repression. (kaist.ac.kr)
  • CHEN, G.-Q. Production of poly-3-hydroxybutyrate by Bacillus sp. (blucher.com.br)
  • however, the Gram-positive B. megaterium does not produce the endotoxins associated with the outer membrane of Gram-negative E. coli . (asm.org)
  • The first P xylA -based gene expression shuttle vector, pWH1520, already comprised all necessary elements for xylose-inducible gene expression in B. megaterium , as well as for replication and antibiotic-based selection in both B. megaterium and E. coli ( 22 ). (asm.org)
  • The material produced by B. megaterium showed an increased specific activity compared to material produced in E. coli . (biomedcentral.com)
  • P. fluorescens, E. cloacae and B. megaterium can produce protease and can therefore be used in the degradation of fish waste. (alliedacademies.org)
  • Isolation of Bacillus megaterium by cultivation in medium, submerged fermentation of palm oil press fibres and effluent by B. megaterium to produce lipase. (sciencedomaininternational.com)
  • v.16 Isolation of Bacillus sp. (koreascience.or.kr)
  • Enrichment culture and serial passaging of deep subsurface fluids from the McElmo Dome scCO2 reservoir in aqueous media under scCO2 headspace enabled the isolation of spore-forming strain Bacillus megaterium SR7. (pacb.com)
  • Previous work has demonstrated that Bacillus megaterium, besides showing an elevated resistance to heavy metals, possesses the ability to remove metals like Ni, Hg, and V from liquid media. (peertechz.com)
  • Thus, the aim of the present study was to determine Bacillus megaterium MNSH1-9K-1 resistance to Al, Ni, V, and Mo, metals that are commonly found in high metal content residues, and also evaluate its Ni removal capability from liquid medium. (peertechz.com)
  • This is the first report about transcriptomic analysis of a B. megaterium to explore the mechanism of arid resistance. (biomedcentral.com)
  • The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L −1 of glucose and 0.5 g L −1 of beef extract at 30°C and pH 7. (hindawi.com)
  • For example, our in silico study of fatty acid biosynthesis and lipid accumulation in B. megaterium highlighted the importance of adopting appropriate carbon sources for fermentation purposes. (chalmers.se)
  • This stu-dy aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. (iium.edu.my)
  • Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15 from stored rice grains exhibited antifungal activity against Aspergillus and Penicillium spp. (asm.org)
  • Among them, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15 were selected as potential biocontrol agents ( 3 - 5 ). (asm.org)
  • Bacillus megaterium (Firmicutes) is ubiquitous in nature. (peerj.com)
  • Polysaccharide synthesis of the levansucrase SacB from Bacillus megaterium is controlled by distinct surface motifs. (helmholtz-hzi.de)
  • Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium. (helmholtz-hzi.de)
  • The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology. (hindawi.com)