Bacillus cereus: A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.Bacillus subtilis: A species of gram-positive bacteria that is a common soil and water saprophyte.Bacillus anthracis: A species of bacteria that causes ANTHRAX in humans and animals.Spores, Bacterial: Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.Bacillus thuringiensis: A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth.Bacillaceae Infections: Infections with bacteria of the family BACILLACEAE.Spores: The reproductive elements of lower organisms, such as BACTERIA; FUNGI; and cryptogamic plants.Bacillus megaterium: A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.Bacterial Proteins: Proteins found in any species of bacterium.Picolinic AcidsEmetics: Agents that cause vomiting. They may act directly on the gastrointestinal tract, bringing about emesis through local irritant effects, or indirectly, through their effects on the chemoreceptor trigger zone in the postremal area near the medulla.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Foodborne Diseases: Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.Oligo-1,6-Glucosidase: An enzyme that catalyzes the endohydrolysis of 1,6-alpha-glucosidic linkages in isomaltose and dextrins produced from starch and glycogen by ALPHA-AMYLASES. EC 3.2.1.10.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Genes, Bacterial: The functional hereditary units of BACTERIA.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Penicillinase: A beta-lactamase preferentially cleaving penicillins. (Dorland, 28th ed) EC 3.5.2.-.Anthrax: An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.Bacillus Phages: Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.Depsipeptides: Compounds consisting of chains of AMINO ACIDS alternating with CARBOXYLIC ACIDS via ester and amide linkages. They are commonly cyclized.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Phospholipases: A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Sphingomyelin Phosphodiesterase: An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Inosine: A purine nucleoside that has hypoxanthine linked by the N9 nitrogen to the C1 carbon of ribose. It is an intermediate in the degradation of purines and purine nucleosides to uric acid and in pathways of purine salvage. It also occurs in the anticodon of certain transfer RNA molecules. (Dorland, 28th ed)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)CephalosporinaseSequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.beta-Amylase: An enzyme that catalyzes the hydrolysis of 1,4-alpha-glycosidic linkages in starch, glycogen, and related polysaccharides and oligosaccharides so as to remove successive beta-maltose units from the non-reducing ends of the chains. EC 3.2.1.2.Enterotoxins: Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.Gram-Positive Bacterial Infections: Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Panophthalmitis: Acute suppurative inflammation of the inner eye with necrosis of the sclera (and sometimes the cornea) and extension of the inflammation into the orbit. Pain may be severe and the globe may rupture. In endophthalmitis the globe does not rupture.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Demeclocycline: A TETRACYCLINE analog having a 7-chloro and a 6-methyl. Because it is excreted more slowly than TETRACYCLINE, it maintains effective blood levels for longer periods of time.Chlortetracycline: A TETRACYCLINE with a 7-chloro substitution.Oxytetracycline: A TETRACYCLINE analog isolated from the actinomycete STREPTOMYCES rimosus and used in a wide variety of clinical conditions.Quality Control: A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)Streptomyces aureofaciens: An actinomycete from which the antibiotic CHLORTETRACYCLINE is obtained.

Utilization of exogenous purine compounds in Bacillus cereus. Translocation of the ribose moiety of inosine. (1/1270)

Intact cells of Bacillus cereus catalyze the breakdown of exogenous AMP to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. Inosine hydrolase was not detectable, even in crude extracts. Inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. Even though the equilibrium of the phosphorolytic reaction favors nucleoside synthesis, exogenous inosine (as well as adenosine and AMP) is almost quantitatively transformed into external hypoxanthine, since ribose 1-phosphate is readily metabolized inside the cell. Most likely, the translocated ribose 1-phosphate enters the sugar phosphate shunt, via its prior conversion into ribose 5-phosphate, thus supplying the energy required for the subsequent uptake of hypoxanthine in B. cereus.  (+info)

A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis. (2/1270)

Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment with AluI distinguished B. anthracis from the other species of the B. cereus group.  (+info)

Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4. (3/1270)

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.  (+info)

Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. (4/1270)

The performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. The systems assessed were the MicroLog system (Biolog Inc., Hayward, Calif.), the Microbial Identification System (MIS; MIDI Inc., Newark, Del.), the VITEK system (bioMerieux Vitek, Hazelwood, Mo.), the MicroScan WalkAway 40 system (Dade-MicroScan International, West Sacramento, Calif.), and the Replianalyzer system (Oxoid Inc., Nepean, Ontario, Canada). The sensitivities and specificities of these systems for the identification of food-borne isolates of Bacillus cereus, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., and verotoxigenic Escherichia coli were determined with 40 reference positive isolates and 40 reference negative isolates for each pathogen. The sensitivities of these systems for the identification of these pathogens ranged from 42.5 to 100%, and the specificities of these systems for the identification of these pathogens ranged from 32.5 to 100%. Some of the systems had difficulty correctly identifying the reference isolates when the results were compared to those from the reference identification tests. The sensitivity of MIS for the identification of S. aureus, B. cereus, E. coli, and C. jejuni, for example, ranged from 47.5 to 72. 5%. The sensitivity of the Microlog system for the identification of E. coli was 72.5%, and the sensitivity of the VITEK system for the identification of B. cereus was 42.5%. The specificities of four of the five systems for the identification of all of the species tested with the available databases were greater than or equal to 97.5%; the exception was MIS for the identification of C. jejuni, which displayed a specificity of 32.5% when it was tested with reference negative isolates including Campylobacter coli and other Campylobacter species. All systems had >80% sensitivities for the identification of Salmonella species and Listeria species at the genus level. The repeatability of these systems for the identification of test isolates ranged from 30 to 100%. Not all systems included all six pathogens in their databases; thus, some species could not be tested with all systems. The choice of automated microbial identification system for the identification of a food-borne pathogen would depend on the availability of identification libraries within the systems and the performance of the systems for the identification of the pathogen.  (+info)

Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice. (5/1270)

As 16S rRNA sequence analysis has proven inadequate for the differentiation of Bacillus cereus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic marker. The gyrB genes of B. cereus JCM 2152(T), Bacillus thuringiensis IAM 12077(T), Bacillus mycoides ATCC 6462(T), and Bacillus anthracis Pasteur #2H were cloned and sequenced. Oligonucleotide PCR primer sets were designed from within gyrB sequences of the respective bacteria for the specific amplification and differentiation of B. cereus, B. thuringiensis, and B. anthracis. The results from the amplification of gyrB sequences correlated well with results obtained with the 16S rDNA-based hybridization study but not with the results of their phenotypic characterization. Some of the reference strains of both B. cereus (three serovars) and B. thuringiensis (two serovars) were not positive in PCR amplification assays with gyrB primers. However, complete sequencing of 1.2-kb gyrB fragments of these reference strains showed that these serovars had, in fact, lower homology than their originally designated species. We developed and tested a procedure for the specific detection of the target organism in boiled rice that entailed 15 h of preenrichment followed by PCR amplification of the B. cereus-specific fragment. This method enabled us to detect an initial inoculum of 0.24 CFU of B. cereus cells per g of boiled rice food homogenate without extracting DNA. However, a simple two-step filtration step is required to remove PCR inhibitory substances.  (+info)

Semiautomated metabolic staining assay for Bacillus cereus emetic toxin. (6/1270)

This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.  (+info)

Effect of hydrostatic tensile stress on the growth of Escherichia coli and Bacillus cereus. (7/1270)

The specific growth rates of Escherichia coli and Bacillus cereus were measured for growth media in a flask, a lens-plate arrangement simulating an isolated capillary space, and a lens-plate arrangement under hydrostatic tensile stress. The specific growth rates of the bacteria were the same for the flask and lens-plate arrangement without hydrostatic tensile stress, but were enhanced when the growth media were subjected to hydrostatic tensile stress. The enhanced specific growth rates reached steady values at a tensile stress of 40 pascals. The effect was observed up to tensile stresses of around 100 pascals. The maximum increase in specific growth rate was 25% for E. coli and 22% for B. cereus.  (+info)

Isolation of stable ribosomal subunits from spores of Bacillus cereus. (8/1270)

Analyses of ribosomes extracted from spores of Bacillus cereus T by a dryspore disruption technique indicated that previously reported defects in ribosomes from spores may arise during the ribosome extraction process. The population of ribosomes from spores is shown to cotain a variable quantity of free 50S subunits which are unstable, giving rise to slowly sedimenting particles in low-Mg2+ sucrose gradients and showing extremely low activity in in vitro protein synthesis. The majority of the ribosomal subunits in spores, obtained by dissociation of 70S ribosomes and polysomes, are shown to be as stable as subunits from vegetative cells, though the activity of spore polysomes was lower than that of vegetative ribosomes. In spite of the instability and inactivity of a fraction of the spore's ribosomal subunits, the activity of the total population obtained from spores by the dry disruption technique was 32% of vegetative ribosome activity, fivefold higher than previously obtained with this species. The improvement in activity and the observed variability of subunit destabilization are taken as evidence for partial degradation of spore ribosomes during extraction.  (+info)

The Bacillus cereus sensu lato group currently includes seven species (B. cereus, B. anthracis, B. mycoides, B. pseudomycoides, B. thuringiensis, B. weihenstephanensis and B. cytotoxicus) that recent phylogenetic and phylogenomic analyses suggest are likely a single species, despite their varied phenotypes. Although horizontal gene transfer and insertion-deletion events are clearly important for promoting divergence among these genomes, recent studies have demonstrated that a major basis for phenotypic diversity in these organisms may be differential regulation of the highly similar gene content shared by these organisms. To explore this hypothesis, we used an in silico approach to evaluate the relationship of pathogenic potential and the divergence of the SigB-dependent general stress response within the B. cereus sensu lato group, since SigB has been demonstrated to support pathogenesis in Bacillus, Listeria and Staphylococcus species. During the divergence of these organisms from a common
Bacillus cereus from the Bacillus cereus group species, which consist of: Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus weihenstephanensis, Bacillus mycoides and Bacillus pseudomycoides is one of the most frequently isolated bacterial foodborne pathogens. Growth of B. cereus results in production of several highly active toxins therefore, consumption of food containing 105-106 bacteria (spores)/g or toxins, is sufficient to cause emetic and diarrhoeal syndromes. The most common source of this bacterium is milk and mixed food products that include milk powder, thus is of particular concern in the baby formula industry. In this study 138 strains of B. cereus group spp. were characterized based on their phenotypic and genotypic features. The study developed unique DNA primers for use in PCR and these were then tested via real-time PCR (RT-PCR): (i) the motB gene encoding the flagellar motor protein MotB was used as a PCR primer target. (ii) New primers and probes, targeting ...
TY - JOUR. T1 - Linking Bacillus cereus genotypes and carbohydrate utilization capacity. AU - Warda, Alicja K.. AU - Siezen, Roland J.. AU - Boekhorst, Jos. AU - Wells-Bennik, Marjon H.J.. AU - de Jong, Anne. AU - Kuipers, Oscar P.. AU - Nierop Groot, Masja N.. AU - Abee, Tjakko. PY - 2016. Y1 - 2016. N2 - We characterised carbohydrate utilisation of 20 newly sequenced Bacillus cereus strains isolated from food products and food processing environments and two laboratory strains, B. cereus ATCC 10987 and B. cereus ATCC 14579. Subsequently, genome sequences of these strains were analysed together with 11 additional B. cereus reference genomes to provide an overview of the different types of carbohydrate transporters and utilization systems found in B. cereus strains. The combined application of API tests, defined growth media experiments and comparative genomics enabled us to link the carbohydrate utilisation capacity of 22 B. cereus strains with their genome content and in some cases to the panC ...
Bacillus thuringiensis is widely used as a bioinsecticide due to its ability to form parasporal crystals containing proteinaceous toxins. It is a member of the Bacillus cereus sensu lato, a group with low genetic diversity but produces several promising antimicrobial compounds. B. thuringiensis DNG9, isolated from an oil-contaminated slough in Algeria, has strong antibacterial, antifungal and biosurfactant properties. Here, we report the 6.06 Mbp draft genome sequence of B. thuringiensis DNG9. The genome encodes several gene inventories for the biosynthesis of bioactive compounds such as zwittermycin A, petrobactin, insecticidal toxins, polyhydroxyalkanoates and multiple bacteriocins. We expect the genome information of strain DNG9 will provide another model system to study pathogenicity against insect pests, plant diseases, and antimicrobial compound mining and comparative phylogenesis among the Bacillus cereus sensu lato group.
Haemolysin BL (HBL) is a Bacillus cereus toxin composed of a binding component, B, and two lytic components, L1 and L2. HBL is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of B. cereus. The three genes encoding the HBL components constitute an operon and are transcribed from a promoter 608 bp upstream of the hblC translational start site. The first gene of the hbl operon, hblC, in the B. cereus type strain, ATCC 14579, was inactivated in this study. Inactivation of hblC strongly reduced both the enterotoxigenic activity of B. cereus ATCC 14579 and the haemolytic activity against sheep erythrocytes, while maintaining full haemolytic activity against human erythrocytes.
Bacillus cereus ATCC ® 10987D-5™ Designation: Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application:
Bacillus cereus ATCC ® 10987D-5™ Designation: Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application:
The Bacillus cereus sensu lato group consists of six species (B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis, and B. weihenstephanensis). While classical microbial taxonomy proposed these organisms as distinct species, newer molecular phylogenies and comparative genome sequencing suggests that these organisms should be classified as a single species (thus, we will refer to these organisms collectively as the Bc species-group). How do we account for the underlying similarity of these phenotypically diverse microbes? It has been established for some time that the most rapidly evolving and evolutionarily flexible portions of the bacterial genome are regulatory sequences and transcriptional networks. Other studies have suggested that the sigma factor gene family of these organisms has diverged and expanded significantly relative to their ancestors; sigma factors are those portions of the bacterial transcriptional apparatus that control RNA polymerase recognition for promoter
Bacillus cereus is a Gram positive rod-shaped aerobic, endospore-forming bacterium. Strains of B. cereus are widely distributed in the environment, mainly in soil, from where they easily spread to many types of foods, especially of vegetable origin, as well as meat, eggs, milk, and dairy products. This bacterium is one of the leading causes of food poisoning in the developed world. B. cereus causes two types of food-borne intoxications. One type is characterized by nausea and vomiting and abdominal cramps and has an incubation period of 1 to 6 hours. This is the "short-incubation" or emetic form of the disease. The second type is manifested primarily by abdominal cramps and diarrhea with an incubation period of 8 to 16 hours. This type is referred to as the "long-incubation" or diarrheal form of the disease [1, 2].. Different strategies may be employed to prevent B. cereus poisoning, like heating food above 75°C before use to kill vegetative cells. However, increasing trends for use of packed ...
The Bacillus cereus group of bacteria comprises soil-dwelling saprophytes but on occasion these bacteria can cause a wide range of diseases in humans, including food poisoning, systemic infections and highly lethal forms of anthrax. While anthrax is almost invariably caused by strains from a single evolutionary lineage, Bacillus anthracis, variation in the virulence properties of strains from other lineages has not been fully addressed. Using multi-locus sequence data from 667 strains, we reconstructed the evolutionary history of the B. cereus group in terms of both clonal inheritance and recombination. The strains included 155 clinical isolates representing B. anthracis, and isolates from emetic and diarrhoeal food poisoning, septicaemia and related infections, wound, and lung infections. We confirmed the existence of three major clades and found that clinical isolates of B. cereus (with the exception of emetic toxin-producing strains) are evenly distributed between and within clades 1 and 2. ...
Net weight: 130 grams. "A sample of the above product, collected at a supermarket in Yuen Long, was found to be contaminated with Bacillus cereus last week. During follow-up investigation on the same day, the CFS collected another sample from the same batch of the product from another supermarket in Yuen Long for testing. The test result showed that the sample contained Bacillus cereus at a level of 200,000 per gram," a CFS spokesman said.. Upon notification of the contamination of the affected batch of bottled preserved bean curd with Bacillus cereus last week, the Centre urged the public and the trade not to eat or sell the product in question, and contacted the importer/distributor concerned immediately to trace the distribution of the food item concerned and instructed it to recall the affected batch of the product. CFS staff also conducted inspections at local retail outlets afterwards and no affected product was found available for sale.. According to the "Microbiological Guidelines for ...
Discrimination by automatic ribotyping and random amplified polymorphic DNA PCR, RAPD, was compared for 40 different B. cereus dairy isolates, 4 different B. mycoides isolates and 6 culture collection strains. RAPD-PCR has previously shown to be useful for tracing contamination routes of B. cereus to milk. Automatic ribotyping using EcoRI and PvuII separated the B. cereus and B. mycoides isolates/strains into 36 different ribotypes. RAPD-typing with primers generated 40 different RAPD-profiles. However, 17 isolates clustered into eight groups, irrespective of the primer and restriction enzyme used, and in all but one case, the isolates with the same pattern were isolated from the same dairy. Automatic ribotyping proved to be a useful, standardized and quick method to discriminate between B. cereus strains, only slightly less discriminatory than RAPD-typing.. ...
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.
Bacillus cereus is widespread in nature and frequently isolated from soil and growing plants . In addition to rice, pasta, and spices, dairy products are among the most common food vehicles for B. cereus. There are two types of B. cereus foodborne illness. The first type, which is caused by an emetic toxin, results in vomiting, whereas the second type, which is caused by enterotoxin(s), results in diarrhea. Little is known about susceptible populations, but the more severe types of the illness have occasionally involved young athletes (19 years) or the elderly (60 years). The emetic toxin, causing vomiting, had been isolated and characterized, whereas the diarrheal disease is caused by one or more enterotoxins. The spore of B. cereus is an important factor in foodborne illness. First, the B. cereus spore is more hydrophobic than any other Bacillus spp. spores, which enables it to adhere to several types of surfaces. Neither of the two commercial immunoassays available for enterotoxin detection can
SUMMARY: Growth of an oligosporogenic strain of Bacillus cereus T was studied in continuous culture. The maximum specific growth rate obtained was 0.47 h−1. RNA content increased linearly with increasing growth rate. Protein content decreased at high growth rates, but the DNA content of the culture was not affected by the growth rate. Activities of enzymes involved in glutamate utilization and energy production increased with increasing growth rate. The maximum specific activity for all enzymes assayed was obtained at a dilution rate of about 0.30 h−1. Changing the concentration of zinc or manganese in the medium by as little as 10−7 M from the concentration optimum for growth caused a significant reduction in the steady-state bacterial population. Zinc appeared to affect the efficiency of conversion of substrate to biomass. Manganese was very toxic at concentrations slightly above the concentration optimum for a specific growth rate.
Abstract: Bacterial strains isolated from the gut of the local termite Coptotermes curvignathus were inoculated into a buffered medium containing minerals and Whatman filter paper as the sole carbon source to observe the ability of the bacteria to digest solid substrate. The bacteria were Bacillus cereus strain Razmin A, Enterobacter aerogenes strain Razmin B, Enterobacter cloacae strain Razmin C, Acinetobacter strain Raminalimon and Chryseobacterium kwangyangense strain Cb. The Gen Bank NCBI/EMBL accession numbers for the bacterial strains were EU294508, EU305608, EU305609, EU332791 and EU169201, respectively. The ability of bacterial cultures to grow in this medium as well as to digest the filter paper was determined by visual observation after 30 days. All bacterial cultures showed growth as the medium turned cloudy and the filter paper became macerated. Chryseobacterium kwangyangense strain Cb showed yellow pigmented colonies on the filter paper. Bacillus cereus strain Razmin A showed clumps ...
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. part 2 Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples
Using state-of-the-art genome sequence-based phylogenomic methods, we elucidated the taxonomy of the important Bacillus cereus group. The article is published under an open access license in Scientific Reports.. ...
Bacillus cereus strain BP01 is a microbial plant regulator that is used on cotton to produce higher quality cotton at lower cost. ...
Food-borne intoxications are increasingly caused by the dodecadepsipeptide cereulide (L-O-Val-L-Val-D-O-Leu-D-Ala)3, the emetic toxin produced by Bacillus cereus (Fig. 1A/C). As such intoxications pose a health risk to humans, a comprehensive understanding on the chemodiversity of this toxin is mandatory for the reliable risk assessment of B. cereus toxins in foods. A fast and robust high-throughput UPLC-ToF-MS profiling method was developed and successfully applied to discriminate a total of 78 Bacillus cereus strains into no (group 1, Fig. 1A), low/medium (group 2/3) and high (group 4/5) producers of the emetic toxin cereulide. Mass spectrometric screening and multivariate analysis (Fig. 1B) showed a series of at least 18 cereulide variants, among which the previously unknown isocereulides A-G (Fig. 1C) were determined for the first time by means of UPLC-ToF-MS and ion-trap MSn sequencing, 13C-labelling experiments, and post-hydrolytic dipeptide and enantioselective amino acid analysis. Most ...
Other names: ATCC 14579, B. cereus, BCRC 10603, Bacillus cereus, Bacillus endorhythmos, Bacillus medusa, Bacillus sp. 2479, Bacillus sp. BS2(2013b), Bacillus sp. BV4, Bacillus sp. JKR50, Bacillus sp. JKR62, Bacillus sp. JP44SK22, Bacillus sp. JP44SK37, Bacillus sp. JP44SK43, Bacillus sp. JP44SK45, Bacillus sp. JSG1(2014), Bacillus sp. KER 17, Bacillus sp. MZ-01, Bacillus sp. PXDK-1, Bacillus sp. Pf-1, Bacillus sp. V3, Bacillus sp. mmm86, CCM 2010, CCRC 10603, CCUG 7414, CIP 66.24, DSM 31, IAM 12605, IFO 15305, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504 ...
Other names: ATCC 14579, B. cereus, BCRC 10603, Bacillus cereus, Bacillus endorhythmos, Bacillus medusa, Bacillus sp. 2479, Bacillus sp. BS2(2013b), Bacillus sp. BV4, Bacillus sp. JKR50, Bacillus sp. JKR62, Bacillus sp. JP44SK22, Bacillus sp. JP44SK37, Bacillus sp. JP44SK43, Bacillus sp. JP44SK45, Bacillus sp. JSG1(2014), Bacillus sp. KER 17, Bacillus sp. MZ-01, Bacillus sp. PXDK-1, Bacillus sp. Pf-1, Bacillus sp. V3, Bacillus sp. mmm86, CCM 2010, CCRC 10603, CCUG 7414, CIP 66.24, DSM 31, IAM 12605, IFO 15305, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504 ...
7.7. Mean values of total bacterial counts were generally less than 105 CFU/g except for brand "F" which contained about 103 CFU/g. Wide variation in the percentage of sporeformers in total count was observed. Mean values of spore counts ranged from 103 to 104 CFU/g. Bacillus cereus was found in variable densities; mean values hardly exceeding 104/g, it was not detected in some samples. Generally, B. Cereus represented 20-70% of the total spore counts. Yeast colony mean counts around 102 CFU/g. Enterobacteriaceae, molds coliforms, Staphylococcus aureus, Streptococcus faecalis and salmonellae could not be detected in all samples. D10 value of B. cereus was 2.85 KGy, while the D10 values at 85, 90, 95 and 100C were found to be 15.0, 9.5, 8.5 and 2.5 min, respectively. Preirradiation treatment of B. cereus spores had a synergistic effect with heat treatment ...
Bacillus Cereus Bacillus cereus is a rod-shaped gram- positive bacillus that can be found in food, dust, dirt and sometimes soil. It is an aerobe and a
Bacillus cereus occurs naturally in most foods, but usually not in high enough numbers to make people sick. Bacillus cereus survives the drying process by producing resistant spores. When potato flakes are rehydrated, the spores can germinate and bacteria multiply. These can produce a toxin if the food is not eaten immediately, and is left sitting at room temperature for a few hours.. Again, further cooking or reheating will not get rid of this toxin.. ...
Effect of Low Dose γ-Irradiation on the Fate and Cell Envelope of Bacillus cereus, Escherichia coli, and Salmonella Typhimurium - ${\gamma}$-irradiation;cell envelop damage;antibiotic resistance;Bacillus cereus;Escherichia coli;Salmonella Typhimurium;
In a statement late this afternoon, NYS Health Department spokesman JP OHare says the department "has identified Bacillus cereus in patient clinical specimens, as well as from refried beans collected from Mighty Taco restaurants, as part of an investigation into gastrointestinal illness among several patrons of certain Mighty Taco restaurants. Bacillus cereus is a type of bacteria that produces a toxin that causes illness, usually resulting in vomiting within 30 minutes to 6 hours after eating contaminated food. This is consistent with the symptoms described by those who became ill shortly after eating at Mighty Taco ...
A common active site of polyhydroxyalkanoate synthase from Bacillus cereus YB-4 is involved in polymerization and alcoholysis reactionsA common active site of polyhydroxyalkanoate synthase from Bacillus cereus YB-4 is involved in polymerization and alcoholysis reactions ...
Home testing and Bacillus cereus type I food poisoning, diagnostic tests, self assessment, and other tools and products in relation to Bacillus cereus type I food poisoning.
Bacillus cereus type I food poisoning is it contagious? Contagiousness of Bacillus cereus type I food poisoning including infectiousness, transmission, and contagion methods and vectors.
Spore-forming bacteria, Bacillus sp., frequently been associated with the contamination of rice and other starchy products. Spores are more resistant to antimicrobial treatments than its vegetative cells. The extract of Indonesian bay leaf (Syzygium polyanthum L.) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of B. cereus isolated from rice (25 strains). The results showed that S. polyanthum L. extract was able to inhibit the growth of vegetative cells of all B. cereus isolates with MICs ranged from 0.16 to 0.63 mg/mL and can kill with MBCs ranged from 0.31 to 2.50 mg/mL. The bactericidal endpoint for B. cereus BC-NP.8 in time kill curve was at 1.25 mg/mL (8× MIC) after 4 h of incubation while for B. cereus ATCC 33019 was at 2.50 mg/mL (8× MIC). The sporicidal activity of S. polyanthum L. extract was not affected by different temperatures treatment and alteration of the pHs of extract. Therefore, this indicates that the extract was stable ...
If you ever thought you had the 24-hour flu, you may have actually had a foodborne illness caused by the bacteria Bacillus cereus. Foods that have not been properly stored at safe cold or hot temperatures are the main sources of the pathogen. Cold foods should be kept cold (below 40 degrees F), even during food service, and hot foods should be hot, which means above 140 degrees F. Proper temperature control can be a problem when large quantities of food are prepared and refrigeration space is in short supply.
If you ever thought you had the 24-hour flu, you may have actually had a foodborne illness caused by the bacteria Bacillus cereus. Foods that have not been properly stored at safe cold or hot temperatures are the main sources of the pathogen. Cold foods should be kept cold (below 40 degrees F), even during food service, and hot foods should be hot, which means above 140 degrees F. Proper temperature control can be a problem when large quantities of food are prepared and refrigeration space is in short supply.
4ESB: Crystal Structures of Two Transcriptional Regulators from Bacillus cereus Define the Conserved Structural Features of a PadR Subfamily.
international flight for example, where many people can become sick in a short period of time. While foodborne illness associated with air travel is quite uncommon, foodborne outbreaks have been recorded by the airline industry as a result of improper testing or handling of food. When this does occur, it can have serious implications for passengers and crew members and means quality, safe, in-flight catering must rely on high standards of food preparation and storage; this applies at the airport kitchens (or at subcontractors facilities) and on the aircraft.. Bacillus cereus has also been recognized as a cause of potentially fatal systemic infections outside the GI tract, particularly in high-risk patients, such as infants, immune-compromised individuals, and chronically debilitated or elderly patients. These infections include bacteremia, septicemia, meningitis, cerebral abscess, pneumonia, and endocarditis.. The risk of illness after ingestion of vegetative B. cereus cells is influenced by ...
international flight for example, where many people can become sick in a short period of time. While foodborne illness associated with air travel is quite uncommon, foodborne outbreaks have been recorded by the airline industry as a result of improper testing or handling of food. When this does occur, it can have serious implications for passengers and crew members and means quality, safe, in-flight catering must rely on high standards of food preparation and storage; this applies at the airport kitchens (or at subcontractors facilities) and on the aircraft.. Bacillus cereus has also been recognized as a cause of potentially fatal systemic infections outside the GI tract, particularly in high-risk patients, such as infants, immune-compromised individuals, and chronically debilitated or elderly patients. These infections include bacteremia, septicemia, meningitis, cerebral abscess, pneumonia, and endocarditis.. The risk of illness after ingestion of vegetative B. cereus cells is influenced by ...
Because of the very similar physiological properties and base sequences of the 16S rRNAs of Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis, some taxonomists question the validity of separating these organisms into distinct species. DNA relatedness studies based on spectrophotometrically measured renaturation rates were carried out to determine the taxonomic relationships of the three species. A study of 58 strains revealed that the levels of relatedness between B. cereus and B. mycoides and between B. cereus and B. thuringiensis ranged from 22 to 44% and from 59 to 69%, respectively. On the basis of the moderately high levels of DNA relatedness which we determined, B. cereus and B. thuringiensis appeared to be genetically related but taxonomically distinct entities. The B. mycoides group was genetically distantly related to the B. cereus group and represented a separate taxon. Furthermore, our data indicated that the B. mycoides group consists of two genetically distinct groups, ...
Bacillus cereus causes two types of food poisoning. One of its toxins causes vomiting and another causes diarrhea. Some unlucky people get both types at once.
Sammendrag bcr1 er et ~155 bp langt ikke kodende repetert element, funnet kun i Bacillus cereus-gruppen, og spesifikt på kromosomet (det er aldri funnet på plasmid). Dette repeterte elementet er ofte å finne i intergenetiske regioner, og dets organisering i kromosomale loci er generelt lite konservert fra en stamme til en annen. Organiseringen av gener som flankerer bcr1 er derimot oftest konservert. bcr1 har terminale direkte repeterte sekvenser (TTTAT), et trekk som er typisk for visse typer mobile elementer. Det er vist in silico at bcr1 har evne til dannelse av stabil sekundærstruktur på RNA-nivå. Kompensatoriske mutasjoner er påvist, og dette kan bety at opprettholdelse av denne strukturen er meget viktig for bcr1- elementets integritet og/eller funksjon.. Repeterte elementer som ikke koder for proteiner er ofte funnet spredt rundt i ulike bakterielle genom. Mange av disse elementene kan ha modifiserende effekt på uttrykk av gener, eksempelvis er dette vist for REP i E. coli og ...
The Bacillus cereus Group consists of closely-related bacteria, including pathogenic or harmless strains, and whose species can be positioned along the seven phylogenetic groups of Guinebretière et al. (I-VII). They exhibit different growth-temperature ranges, through thermotolerant to psychrotolerant thermotypes. Among these, B. cytotoxicus is an atypical thermotolerant and food-poisoning agent affiliated to group VII whose thermotolerance contrasts with the mesophilic and psychrotolerant thermotypes associated to the remaining groups I-VI. To understand the role of fatty acid (FA) composition in these variable thermotypes (i.e. growth behavior vs temperatures), we report specific features differentiating the FA pattern of B. cytotoxicus (group VII) from its counterparts (groups I-VI). The FA pattern of thermotolerant group VII (B. cytotoxicus) displayed several specific features. Most notably, we identified a high ratio of the branched-chain FAs iso-C15/iso-C13 (i15/i13) and the absence of the
Anti-bacterial effect:. Choleve is rich in theaflavins. A group of researchers from US department of Agriculture conducted a research to evaluate the antimicrobial activities of theaflavins from tea. The bacteria strain they used is Bacillus cereus (strain RM3190). The results demonstrate that theaflavins showed antimicrobial activities at nanomolar level; they were more active than were medicinal antibiotics, such as tetracycline or vancomycin, at comparable concentrations.. Reference:. Friedman M, Henika PR, Levin CE, Mandrell RE, Kozukue N: Antimicrobial activities of tea catechins and theaflavins and tea extracts against Bacillus cereus. Journal of Food Protection 2006; 69(2): 354-361. Anti-viral effect:. Theaflavin derivatives and catechin derivatives are the major polyphenols in black tea and green tea, respectively. Several tea polyphenols, especially those with galloyl moiety, can inhibit HIV-1 replication with multiple mechanisms of action. Researchers showed that the theaflavin ...
Aim: The present study was undertaken to describe the biotyping of Bacillus cereus isolated from different street vended mutton tikka and chutney samples. Materials and Methods: A total of 100 street vended food samples comprising of 60 mutton tikka and 40 chutney samples were tested. Results: The biotype 3 and biotype 4 showed the highest occurrence with, 29.63% and 25.93% isolates falling in these biotypes, respectively. The percentage occurrence of the biotypes 1, 6, 2, 5, and 7 was 14.81%, 11.11%, 7.40%, 7.40% and 3.84%, respectively. The most common found biotypes in Mutton tikka were biotypes 3(29.63%), 4(25.93%), 1(14.81%) and 6(11.11%). The Bacillus cereus strains isolated from chutney samples could be divided into 7 of the 9 possible biotypes. The biotypes 6 and 7 showed the highest occurrence with 38.46% and 30.76% falling in these biotypes, respectively. The biotype 5 and 2 were prevalent to the extent of 23.07%, 7.69%, respectively. The biotypes 3, 4 and 1 were absent. The mean ...
Abstract This study aimed to conduct the isolation, screening and identification of bacteria with a high level of cellulolytic activity from the muddy sediments of mangrove swamps in Thailand. One hundred and ninety aquatic bacterial isolates were isolated from different muddy sediments and eighty one isolates were determined to be cellulolytic bacteria. The cellulolytic bacterium identified as Bacillus cereus JD0404 showed maximum hydrolysis activity on carboxymethylcellulose agar plates. Its cellulolytic performance for CMCase activity, Avicelase activity and β-glucosidase activity was 1.778±0.003U/mL, 0.079±0.001U/mL and 0.048±0.002U/mL, respectively. The optimum temperature and pH for the enzyme activity were determined to be 50°C and 7.0 respectively. The cellulolytic activity was greatly enhanced by Mn2+ and considerably inhibited by EDTA and toluene. Preliminary bioconversion application showed that the B. cereus JD0404 could be used for the
The Litopenaeus vannamei aquaculture industry in India has faced a severe problem with a new type of bacterial diseases namely White Patch Disease (WPD). In day by day the disease causes gradual mortalities and once the severe disease outbreak comes, the farm faced high mortality of more than 70 % within 3 to 5 days. The major symptoms are white opaque patches in the carapace, necrosis, whitish blue coloration, loss of appetite and pale white muscles. Infected shrimp was subjected Gram staining found that the rod shaped bacteria was the pathogen. Genomic identification also confirmed that the causative pathogen is Bacillus cereus WPD (GenBank No: KF673474.1). Also B. cereus WPD had the ability of higher virulence factors including hemolytic activity, lipase activity and high mortality to the L. vannamei and Artemia when challenging at the rate of 104 to 108 cfu/ml.. - A new type of bacterial diseases namely White Patch Disease (WPD) was identified in L. vannamei culture. - The causative pathogen ...
The aim of the present study was to investigate the biochemical characteristics, extracellular enzyme production and enterotoxigenic genes contents of 6 Bacillus cereus and 22 Bacillus thuringiensis strains, isolated from ...
SWISS-MODEL Repository entry for Q72XD7 (GLYA_BACC1), Serine hydroxymethyltransferase. Bacillus cereus (strain ATCC 10987 / NRS 248)
SWISS-MODEL Repository entry for Q73B98 (LEUC_BACC1), 3-isopropylmalate dehydratase large subunit. Bacillus cereus (strain ATCC 10987 / NRS 248)
... It is gram positive rod shaped bacilli with square ends. It contains spores with central spores and is 1x3-4 µm in size.
Clinical manifestation of Bacillus cereus A. Food poisoning Two forms of food poisoning: vomiting disease (emetic form) and diarrheal disease (diarrheal form). Emetic form The emetic form of disease results from the consumption of contaminated rice. Most bacteria are killed during the initial cooking of the rice, but the heat-resistant spores survive. If the cooked […] ...
en] Metallo-beta-lactamases are native zinc enzymes that catalyse the hydrolysis of beta-lactam antibiotics, but are also able to function with cobalt(II) and require one or two nnetal-ions for catalytic activity. The hydrolysis of cefoxitin, cephaloridine and benzylpenicillin catalysed by CoBcII (cobalt-substituted beta-lactamase from Bacillus cereus) has been studied at different pHs and metal-ion concentrations. An enzyme group of pK(a) 6.52 +/- 0.1 is found to be required in its deprotionated form for metal-ion binding and catalysis. The species that results from the loss of one cobalt ion from the enzyme has no significant catalytic activity and is thought to be the mononuclear CoBcII. It appears that dinuclear CoBcII is the active form of the enzyme necessary for turnover, while the mononuclear CoBcII is only involved in substrate binding. The cobalt-substituted enzyme is a more efficient catalyst than the native enzyme for the hydrolysis of some beta-lactam antibiotics suggesting that the ...
Michelet, Nathalie ; Granum, Per Einar ; Mahillon, Jacques. Bacillus cereus enterotoxins, bi- and tri- component cytolysins and other haemolysins. In: Author : J, Alouf ; Editor(s) : Alouf and Popoff, The Comprehensive Sourcebook of Bacterial Protein Toxins, Elsevier 2005, p. 779- ...
Bacillus cereus is an aerobic spore-forming bacterium that is commonly found in soil, on vegetables, and in many raw and processed foods.
Semantic Scholar extracted view of [Role of aerobic sporogenic bacteria (Bacillus cereus) in food products]. by Kovacs Csaba et al.
BioAssay record AID 40322 submitted by ChEMBL: In vitro minimum inhibitory concentration against Bacillus cereus (GC 4561) assay organism.
Read "Characterization and genome analysis of the Bacillus cereus -infecting bacteriophages BPS10C and BPS13, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Several cysteinyl peptides have been synthesised and shown to be reversible competitive inhibitors of the Bacillus cereus metallo-β-lactamase. The pH dependence of pKi indicates that the thiol anion displaces hydroxide ion from the active site zinc(II). , -Peptides bind to the enzyme better than other diastereoisomers, which is compatible with the predicted stereochemistry of the active site. ...
Domain combinations containing the CutC-like superfamily in Bacillus cereus E33L. Domain architectures illustrate each occurrence of the CutC-like superfamily.
Inhibitors of β-lactamases are important to the treatment of infectious diseases when used in conjunction with a β-lactam antibiotic. Current inhibitors of β-lactamase such as clavulanic acid, sulbactam, and tazobactam perform efficiently overall but due to developing bacterial resistances to these inhibitors, new inhibitors need to be discovered. SELEX procedures were used to isolate ssDNA aptamers capable of binding to the enzyme active site and consequently inhibit the action of β-lactamase I from Bacillus cereus 569/H/9. A 22 base ssDNA aptamer was discovered to have an inhibition pattern consistent with reversible competitive inhibition. These results prompted further study of a hairpin loop of 10 bases and a linear 11 base ssDNA aptamer which were truncated forms of the original 22 base aptamer. The 11 base aptamer failed to show any inhibition against β-lactamase I, whereas the 10 base aptamer showed competitive reversible inhibition ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|225862150|ref|YP_002747528.1| from Bacillus cereus 03BB102. Plus protein sequence and external database links.
Avaliação da prevalência de bacillus cereus em leite pasteurizado e comportamento desse microrganismo durante armazenamento sob temperatura crítica de ...
Bacillus cereus Catheter-Related Infection in Acute Lymphoblastic Leukemia (ALL) Patient: A Case Report and Review of Literature Abstract.
The bactericidal properties of cow urine have been known to humans from a long time. Cow urine is an effective antibacterial agent against a broad spectrum of Gram-negative and Gram-positive bacteria. In this work, we explored photoactivated cow urine as a potential antimicrobial agent. The antimicrobial activity of cow urine was tested by agar well method using Gram-positive bacteria Bacillus cereus (MTCC-1305), Staphylococcus aureus (MTCC-3160) and Gram-negative bacteria Salmonella typhimurium (MTCC-1253), Aeromonas hydrophila (MTCC-1739), Enterobacter aerogenes (MTCC-2823), Micrococcus luteus (MTCC-1809). Photoactivated urine showed highest antimicrobial activity against Gram-positive Bacillus cereus and Gram-negative bacteriaAeromonas hydrophila . Bactericidal effect showed against various bacteria depended on the concentration of photoactivated urine and the type of bacteria.. ...
Jan 22, 2001 (CIDRAP News) The case of a postal inspector who handled anthrax-tainted equipment and came down with a lingering anthrax-like illness but never had clear evidence of the organism in his body continues to puzzle his physicians nearly 3 months after he first got sick.
Though Bacillus species has frequently been reported to cause nosocomial pseudobacteremia or pseudo-outbreaks, few previous reports have used molecular methods to document the clonality of the epidemic strains and to trace the source of contamination (11). Among these pseudoepidemics due to Bacillusspecies, B. cereus was identified on a few occasions and has been demonstrated to be associated with contamination of air filtration systems in pediatric and maternity units, ventilator equipment in an intensive care unit, and a water bath in a microbiology laboratory (1, 4, 11, 14, 20). Two important points have been elucidated in the present study. First, this is the first report to document that a nosocomial pseudoepidemic caused by B. cereus was due to contaminated ethyl alcohol used as a skin disinfectant and successfully traced back to the source of contamination from the alcohol supplier outside the hospital. Second, RAPD analysis by the APPCR technique and MIC antibiotyping provided ...
1BC2: Crystal structure of the zinc-dependent beta-lactamase from Bacillus cereus at 1.9 A resolution: binuclear active site with features of a mononuclear enzyme.
My humble apologies but there was a significant typo in the post I sent yesterday concerning the repeated food poisoning episodes. The Bacillus cereus count for the milk was ,5000 CFU/g (and not ,10 CFU/g as posted yesterday.) The (corrected) results are as follows: , Lab Results , , Date: June 1, 1995 , , Cheese Units Methods , , Aerobic Plate Count 35 deg. C 2.4E6 CFU/g pour , Presumptive Coliforms ,3 MPN/g mpn fd , Faecal Coliforms Nil MPN/g mpn fd , Coag positive Staphylococci ,10 CFU/g spread , Bacillus Cereus ,10 CFU/g spread , Clostridium perfringens ,10 CFU/g spread , , Mushrooms , , Aerobic Plate Count 35 deg. C 1.7E6 CFU/g pour , Presumptive Coliforms 1100 MPN/g mpn fd , Faecal Coliforms ,3 MPN/g mpn fd , Coag positive Staphylococci ,10 CFU/g spread , Bacillus Cereus ,10 CFU/g spread , Clostridium perfringens ,10 CFU/g spread , , Milk , , Aerobic Plate Count 35 deg. C 7.2E4 CFU/g pour , Presumptive Coliforms ,3 MPN/g mpn fd , Faecal Coliforms Nil MPN/g mpn fd , Coag positive ...
A close relative of Bacillus cereus isolated from local soil samples was previously shown to inhibit the growth of P. aeruginosa when grown competitively using a cross streak method. Organic extracts from this bacteria has been tested against several ESKAPE pathogens using both direct application and TLC-bioautography. Further work to isolate and test the antimicrobial compounds produced by this bacteria closely related to Bacillus cereus will be discussed. Various strains of Bacillus cereus have been commonly found in soil and in food.
Background: Bacillus cereus is a Gram-positive spore-forming bacterium, which causes food poisoning. Spores enable the persistence of B. cereus in the environment, and B. cereus strains can tolerate adverse environmental conditions, such as temperature and insufficient nutrients. B. cereus causes food poisoning via the production of two enterotoxins. Most isolates produce toxins leading to diarrhea (enterotoxins) and vomiting (emetic forms). Diarrhea is caused by the production of three different heat-labile enterotoxins: HBL, NHE, and cytotoxin K. A heat-stable toxin, cereulide, is responsible for emesis. ...
2-Oxocarboxylic acids, also called 2-oxo acids and alpha-keto acids, are the most elementary set of metabolites that includes pyruvate (2-oxopropanoate), 2-oxobutanoate, oxaloacetate (2-oxosuccinate) and 2-oxoglutarate. This diagram illustrates the architecture of chain extension and modification reaction modules for 2-oxocarboxylic acids. The chain extension module RM001 is a tricarboxylic pathway where acetyl-CoA derived carbon is used to extend the chain length by one. The chain modification modules RM002 (including RM032) and RM033, together with a reductive amination step (RC00006 or RC00036), generate basic and branched-chain amino acids, respectively. The modification module RM030 is used in the biosynthesis of glucosinolates, a class of plant secondary metabolites, for conversion to oxime followed by addition of thio-glucose moiety. Furthermore, the chain extension from 2-oxoadipate to 2-oxosuberate is followed by coenzyme B biosynthesis in methonogenic archaea ...
Chez les bactéries sporulantes du genre Bacillus, des mécanismes importants tels que la sporulation et la virulence sont régulés par des systèmes de communication cellulaire qui impliquent des peptides de signalisation et des régulateurs de la famille RNPP (Rap, NprR, PlcR, PrgX). Lobjectif de mon travail de thèse a été de déterminer le rôle du régulateur NprR chez les bactéries du groupe B. cereus. Ce travail se divise en trois parties complémentaires. La première partie a consisté à montrer que NprR est impliqué dans un système de communication cellulaire. Nous avons montré que NprR est un régulateur transcriptionnel de début de phase stationnaire qui est dépendant du peptide de signalisation NprX. Associé à NprX, NprR active la transcription du gène nprA qui code pour une protéase extracellulaire. Nous avons démontré que le peptide NprX est sécrété, maturé puis réimporté dans la cellule bactérienne par deux systèmes doligopeptide perméase (Opp et Npp). Une fois
Biofilms can be a persistent problem for those cleaning production equipment in GMP cleanroom facilities. Researchers* at STERIS have shown however that a combination of an effective alkaline cleaning detergent followed by a sporicide is an effective means to address even the most highly resistant biofilm cell populations such as B. cereus
Steps to Identify B. cereus for your microbiology unknown lab report. Paper includes Introduction, Methods, Materials, Results and Discussion/Conclusion.
Steps to Identify B. cereus for your microbiology unknown lab report. Paper includes Introduction, Methods, Materials, Results and Discussion/Conclusion.
This is a bit of fun I did with my children to show them the dangers of eating too many sweets. Bacteria are commonly used as poweful sensors for toxins and environmental pollutants so for this experiment I fed some Gummy Bear sweets to the spreading soil bacterium Bacillus mycoides. As you can see from…
The objectives of this investigation were to produce a novel chitosanase for application in industries and waste treatment. The transformation of chitinous biowaste into valuable bioactive chitooligomers (COS) is one of the most exciting applications of chitosanase. An amphiprotic novel chitosanase from Bacillus mycoides TKU038 using squid pen powder (SPP)-containing medium was retrieved from a Taiwan soil sample, which was purified by column chromatography, and characterized by biochemical protocol. Extracellular chitosanase (CS038) was purified to 130-fold with a 35% yield, and its molecular mass was roughly 48 kDa. CS038 was stable over a wide range of pH values (4-10) at 50 °C and exhibited an optimal temperature of 50 °C. Interestingly, the optimum pH values were estimated as 6 and 10, whereas CS038 exhibited chitosan-degrading activity (100% and 94%, respectively). CS038 had Km and Vmax values of 0.098 mg/mL and 1.336 U/min, separately, using different concentrations of water-soluble chitosan. A
This is a funny gift for any science nerd whose baby poops a lot! Bacillus cereus (B. cereus) is a toxin-producing bacteria that can cause diarrhea. Despite being a serious organism, it has a very pun worthy name! Each letter on this shirt is filled with a different kind of microorganism. Any microbiology fan would get a kick out of this shirt! Material 100% Cotton Durable with vibrant, long-lasting shirt color Style Reinforced 3 snap closure with flat lying seams Sizes 12 Month - 24 Month {Please refer to sizing chart in images}
From a cosmid gene bank of Bacillus cereus GP4 in Escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. These clones contained recombinant cosmids with B. cereus DNA insertions of varying lengths which shared some common restriction fragments. The smallest insertionwas recloned as aPstl fragment into pJKK3-1, a shuttle vector which repücates in Bacillus subtilis and E. coli. When this recombinant plasmid (pJKK3-1 hly-1) was transformed into E. coli, it caused hemolysis on erythrocyte agar plates, but in liquid assays no extemal or intemal hemolytic activity could be detected with the E. coli transformants. B. subtilis carrying the same plasmid exhibited hemolytic activity at Ievels comparable to those ofthe B. cereus donor strain. The hemolysin produced in B. subtilis seemed to be indistinguishable from cereolysin in its sensitivity to cholesterol, activation by dithiothreitol, and inactivation by antibodies raised ...
AIMS: To evaluate the genetic relationship in the Bacillus cereus group by rep-PCR fingerprinting. METHODS AND RESULTS: A collection of 112 strains of the six species of the B. cereus group was analysed by rep-PCR ...
The development of relationships between the pH of a heating medium and the thermal resistance of contaminant microorganisms is important and of a public health significance. A number of mathematical models have been presented in recent years, including that of Mafart and Leguérinel (1998) . However, in this This model , the effect of possible interactions between temperature and pH on D-values was not assessed. The consequences of ignoring interaction terms in models has been assessed and a comparison with Mafarts model that includes an interaction term showed that interaction terms can be neglected and that Mafarts model can be used in thermal process calculations. It appears possible to adopt a standard value of zpH , for example the 3.6 value and the conventional concept of biological destruction value L(T) (ratio of the sterilization value and the exposure time at a fixed heating temperature) may then be extended to L(T,pH) (the same ratio at a fixed temperature with a fixed pH of the heating
Jiménez G, Urdiain M, Cifuentes A, López-López A, Blanch AR, Tamames J, Kämpfer P, Kolstø AB, Ramón D, Martínez JF. 2013. Description of Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and pairwise genome comparisons of the species of the group by means of ANI calculations. Syst Appl Microbiol 36(6):383-91 doi: 10.1016/j.syapm.2013.04.008 ...
Jiménez G, Urdiain M, Cifuentes A, López-López A, Blanch A, Tamames J, Kämpfer P, Kolstø A, Ramón D, Martínez J. 2013A. Descriptionof Bacillus toyonensis sp. nov., a novel species of the Bacillus cereus group, and a pairwise genome comparisons of the species of the group by means of ANI calculations. Elsevier. 36(6):383-391 ...
Second-order rate constants for the alkaline hydrolysis of 3-thiol substituted cephalosporins are independent of the pKa of the thiol over a pKa range of 9. If there is a leaving group at C-3 it is expelled after the -lactam ring is opened and the expulsion of the leaving group does not enhance the rate of -lactam C-N bond fission. The zinc enzyme -lactamase II is about a 100-fold better catalyst than the serine enzyme -lactamase I for the hydrolysis of the same cephalosporin. The second-order rate constant kcat/Km for both -lactamase enzymes shows no dependence on the nature of the substituent at C-3 which is not explicable by the different chemical reactivity of the cephalosporins. There is no evidence for a significant recognition site in either enzyme for the C-3 substituent. The kinetic parameters kcat and Km for the -lactamase I-catalysed hydrolysis may be complicated by the formation of intermediates.. ...
Gram-positive motile rod. Capable of producing endospores. Incubate at 30°C. In nutrient broth. NOTE: Bacterial and fungal cultures will be sold and shipped to institutions only. …
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By: Candess Zona-Mendola There have been a large number of news reports recently of people suffering foodborne illness from holiday ...
Lofstad, Marie; Hammerstad, Marta; Olsbu, Inger Kirstine; Røhr, Åsmund Kjendseth; Hersleth, Hans-Petter & Andersson, K. Kristoffer (2012). Structural and Functional Studies of Nitric Oxide Synthase and Flavodoxines in Bacillus cereus. Vis sammendrag Structural and Functional Studies of Nitric Oxide Synthase and Flavodoxines in Bacillus cereus Marie Lofstad, Marta. Hammerstad, Inger .K. Olsbu, Åsmund .K. Røhr, Hand-Petter.-P. Hersleth and K. Kristoffer.K. Andersson Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindern, Oslo, Norway Marie Lofstad ,[email protected], Nitric Oxide Synthase (NOS) is the enzyme that produces nitric oxide in the body from arginine and oxygen. In mammals three different NOS isoforms with different functions exist: endothelial eNOS, neuronal nNOS and inducible iNOS. The mammalian NOS consists of one oxygenase domain with heme/biopterin (NOSoxy), andone reductase domain (with FMN, FAD and NADPH) and can have a calmodulin domain . The ...
Six months after that inspection, word came from the Colorado health department: A hospital had discovered Triads alcohol wipes were contaminated with Bacillus cereus.. And on Nov. 29, the same four inspectors who had been at the plant in 2009 and earlier in 2010 were sent back to 700 W. North Shore Drive in Hartland. They scoured the plant for weeks and found 46 violations of good manufacturing practices.. A bucket labeled purified water was actually deionized water used to rinse equipment after cleaning. Water pipes leading to vats that made batches of mouth rinse and glycerin suppositories had microbial contamination.. And there, listed as Observation 7 on page 5 of the 30-page report by FDA inspectors, is the one violation most devastating to the Kothari family:. Sterile alcohol prep wipes were found to be contaminated with Bacillus cereus organisms and were released for shipment after confirmation of the results, the inspection report noted.. In the copy of the inspection report ...
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Streptococcus pyrogenes ( MIC = 100 microg/ml ), Bacillus cereus T ( MIC > 100 microg/ml ), Bacillus megaterium ( MIC = 5 microg/ml ), Bacillus subtilis ( MIC = 5 microg/ml ), Staphylococcus aureus ( MIC > 100 microg/ml ...
Perhaps I should have indicated more so the effort I have put in thus far in trying to determine just exactly what my organism was so I wouldnt have been met with such a lack of caring and sarcasm when I merely came here to receive help like everyone else. With this being a basic microbiology course, I have limited knowledge of the various organisms out their but I know when beginning with identification to start with the fact that its 1.) a bacterium, 2.) bacillus (apparently), and 3.) gram-positive. Using this criteria I have used the Bergeys manual and come up with several options but have yet to be able to narrow it down; this being in part because Im having trouble finding a diffenative list of every characteristic for each organism. So far, however, I know it cant be Bacillus cereus because the given organism is non-motile. Same applies for Listeria monocytogenes which is disappointing since I found they are coccobacilli which I thought could be the answer to my shape dilema. From ...
Comparative study on the antibacterial activities of four commercially available antiseptics- Dettol, Hexisol, Oralon and Betadine against staphylococcus aureus, klebsiella pneumoniae , bacillus cereus, and pseudomonas ...
White blood cells engulf bacteria. Coloured trans- mission electron micrograph (TEM) of a section through two polymorph white blood cells (blue) engulfing Bacillus cereus bacteria (magenta). These soil bacteria are a cause of human food poisoning. During phagocytosis the polymorphs (polymorphonuclear granulocytes) capture and then digest foreign particles or, as here, bacteria cells. Polymorphs are part of the immune system that protects the body from invading organisms. Magnification: x15,000 at 6x7cm size. x50,000 at 7.5x9.5 - Stock Image P276/0117
Aim: To synthesize a series of novel compounds namely, 4-(3-((substituted)-1H-pyrazol-3-yl-methylsulphonyl)-5-((3,5,6-trichloro-pyridin-2-yl-oxymethyl)-[1,2,4]triazol-4-yl-methyl)-morpholine (8a-g) and evaluate their antimicrobial activity. Material and Method: The chemical structures of newly synthesized compounds were elucidated by IR, 1H NMR, mass spectral and elemental analysis data. Their antimicrobial activities against Staphylococus aureus NCCS 2079, Bacillus Cereus, NCCS 2106, Escherichia coli NCCS 2065, Pseudomanas aeruginos NCCS 2200, Aspergillus niger ...
Many commensal microorganisms (microorganisms that live on or in other organisms causing no direct benefit or harm) that occur on plant roots and leaves can passively protect plants against microbial pests by competitive exclusion (that is, simply crowding them out). Bacillus cereus has been used as an inoculumon soybean seeds to prevent infection by fungal pathogens in the genus Cercospora ...
This discovery could help design drugs to give the body a better chance against food poisoning. Explore the science behind the video here: A multicomponent toxin from Bacillus cereus incites inflammation and shapes host outcome via the NLRP3 inflammasome
Any chance you have a can of Slim-Fast in your house? Unilever is recalling ALL 11 ounce cans of Slim-Fast ready-to-drink shakes. There is a possibility of contamination with a micro-organism called Bacillus cereus. The press release instructs you to call 1-800-896-9479, however, if you call that number, it will direct you to call 800-754-6327. After the message, you will be instructed to press one and enter your complete name and address, plus the number of ca
Bacillus brevis • Bacillus CereusBacillus megaterium • Bacillus subtilis • Burkholderia cepacia • Campylobacter jejuni • ...
Bacillus cereus infection Bacillus cereus Bacterial pneumonia multiple bacteria Bacterial vaginosis List of bacterial vaginosis ...
Bacillus cereus group: close and polyphyleticEdit. Main article: Bacillus cereus. In a similar way, the Bacillus species (= ... Main article: Bacillus. Another example of a large genus with nested genera is the genus Bacillus, in which the genera ... phylum Firmicutes) belonging to the "B. cereus group" (B. anthracis, B. cereus, B . thuringiensis, B. mycoides, B. ... and gyrB Gene Sequence Analysis to Determine Phylogenetic Relationships of Bacillus cereus Group Microorganisms". Journal of ...
"The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral ... Bacillolysin (EC 3.4.24.28, Bacillus metalloendopeptidase, Bacillus subtilis neutral proteinase, anilozyme P 10, Bacillus ... Feder, J.; Keay, L.; Garrett, L.R.; Cirulis, N.; Moseley, M.H.; Wildi, B.S. (1971). "Bacillus cereus neutral protease". Biochim ... "Crystal structure of neutral protease from Bacillus cereus refined at 3.0 Å resolution and comparison with the homologous but ...
... inhibits the growth of several bacteria strains, e.g. Escherichia coli and Bacillus cereus. Its low toxicity together ... Ultee, A; Smid, E.J (2001). "Influence of carvacrol on growth and toxin production by Bacillus cereus". International Journal ... "Antimicrobial activity of carvacrol toward Bacillus cereus on rice". Journal of food protection. 63 (5): 620-4. PMID 10826719. ...
Sanwal BD & Zink MW (1961). "L-Leucine dehydrogenase of Bacillus cereus". Arch. Biochem. Biophys. 94: 430-435. doi:10.1016/0003 ...
Olsen DB, Hepburn TW, Moos M, Mariano PS, Dunaway-Mariano D (1988). "Investigation of the Bacillus cereus phosphonoacetaldehyde ... identification of 2-phosphonoacetaldehyde as an intermediate in the degradation of 2-aminoethylphosphonate by Bacillus cereus ...
Kellenberger, G.; Kellenberger, E. (1952-01-01). "[Bacteriolysis of a strain of bacillus cereus; evidence in electronic ...
Bacteria having a centrally placed endospore include Bacillus cereus. Sometimes the endospore can be so large the cell can be ... Kadota H, Iijima K (1965). "The X-ray diffraction pattern of spores of Bacillus subtilis". Agric Biol Chem. 29 (1): 80-81. doi: ... There is one report of viable spores of Bacillus marismortui in salt crystals approximately 250 million years old.[4][5] When ... In Bacillus subtilus endospores, the spore coat is estimated to contain more than 70 coat proteins, which are organized into an ...
"A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus". PLOS ONE. 7 (7): e41248. Bibcode:2012PLoSO...741248R. doi: ...
The soil bacterium Bacillus cereus can be used to transform resveratrol into piceid (resveratrol 3-O-beta-D-glucoside). ... "Biotransformation of resveratrol to piceid by Bacillus cereus". J. Nat. Prod. 61 (10): 1313-4. doi:10.1021/np980139b. PMID ...
... is a bacteriophage that infects the spore-forming bacterium Bacillus cereus. Though this lytic phage is a ... Kong, M; Kim, M; Ryu, S (June 2012). "Complete Genome Sequence of Bacillus cereus Bacteriophage PBC1". Journal of Virology. 86 ...
News on cereulide, the emetic toxin of Bacillus Cereus M. A. Andersson; R. Mikkola; J. Helin; M. C. Andersson; M. Salkinoja- ... 2004). "News on cereulide, the emetic toxin of Bacillus Cereus". S. Pitchayawasin; M. Isobe; M. Kuse; T. Franz; N. Agata; M. ... Cereulide is a toxin produced by Bacillus cereus. A cyclic dodecadepsipeptide resembling valinomycin, it contains three repeats ... is an emetic toxin of Bacillus cereus". FEMS Microbiol Lett. 129 (1): 17-20. doi:10.1016/0378-1097(95)00119-P. PMID 7781985. ...
A number of pure cultures, e.g. Trichosporon cutaneum, Bacillus cereus, Klebsiella oxytoca, Pseudomonas sp. etc. individually, ...
... is an antibiotic that has been identified from the bacterium Bacillus cereus UW85. It is a molecule of interest ... Emmert EA, Klimowicz AK, Thomas MG, Handelsman J (January 2004). "Genetics of zwittermicin a production by Bacillus cereus". ... Haiyin, He (April, 1994) "Zwittermicin A, an Antifungal and Plant Protection Agent from Bacillus cereus", Tetrahedron Letters ... April 2007). "Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki ...
Bacillus cereus strain AIMST Nalbe1 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Nalbe7 16S ... Bacillus cereus strain AIMST Nalbe8 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Nalme2 16S ... Bacillus cereus strain AIMST Nalme13 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Ngme4 16S ... Bacillus cereus strain AIMST Ngse7 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Ngse9 16S ...
Oyama, T.; Kusunoki, M.; Kishimoto, Y.; Takasaki, Y.; Nitta, Y. (1999). "Crystal structure of beta-amylase from Bacillus cereus ... "The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251". The Journal of ...
Bacteria having a centrally placed endospore include Bacillus cereus. Sometimes the endospore can be so large the cell can be ... Bacillus subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of ... After the spores were analyzed by microscopy, it was determined that the cells were very similar to Bacillus sphericus which is ... There is one report of viable spores of Bacillus marismortui in salt crystals approximately 250 million years old. When the ...
Hämolysin aus Bacillus cereus,[16] Alveolysin aus Bacillus alvei, Streptolysin O aus Streptococcus pyogenes oder Tetanolysin ... N. Ramarao, V. Sanchis: The pore-forming haemolysins of bacillus cereus: a review. In: Toxins. Band 5, Nummer 6, Juni 2013, S. ... H. Lee, H. Y. Kim: Lantibiotics, class I bacteriocins from the genus Bacillus. In: Journal of microbiology and biotechnology. ...
Staphylococcus aureus, Streptococcus hemolytic, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli Antipyretic. S. ... Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and ...
Little, C.; Otnass, A.-B. (1975). "The metal ion dependence of phospholipase C from Bacillus cereus". Biochim. Biophys. Acta. ...
"Antimicrobial activity of a newly identified bacteriocin of Bacillus cereus". Appl Environ Microbiol. 59 (12): 4313-6. PMC ...
... s are a group of bacteriocins produced by various strains of the bacterium Bacillus cereus. Although all cereins are by ... Bacillus cereus MRX1". J Appl Microbiol. 103 (5): 1621-31. doi:10.1111/j.1365-2672.2007.03395.x. PMID 17953573. ... a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity". FEMS Microbiol. Lett. 178 (2): 337-341. PMID ... a novel bacteriocin produced by Bacillus cereus Bc7". FEMS Microbiol. Lett. 254 (1): 108-15. doi:10.1111/j.1574-6968.2005.00009 ...
"The Arthromitus stage of Bacillus cereus: Intestinal symbionts of animals". Proceedings of the National Academy of Sciences of ... at isolating Arthromitus from termite guts led to the conclusion that Arthromitus is in fact a growth stage of Bacillus cereus ...
Budzik JM, Oh SY, Schneewind O (May 2009). "Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus ... Budzik JM, Marraffini LA, Schneewind O (October 2007). "Assembly of pili on the surface of Bacillus cereus vegetative cells". ... Budzik JM, Oh SY, Schneewind O (December 2008). "Cell wall anchor structure of BcpA pili in Bacillus anthracis". The Journal of ... "Amide bonds assemble pili on the surface of bacilli". Proceedings of the National Academy of Sciences of the United States of ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
Bacillus cereus ATCC ® 11778™ Designation: FDA strain PCI 213 TypeStrain=False Application: Assay of chlortetracycline ... Bacillus cereus Frankland and Frankland (ATCC® 11778™) Strain Designations: FDA strain PCI 213 [ATCC 9634, NCTC 10320, S.A. ... Nucleotide (GenBank) : AF238886 Bacillus cereus strain ATCC 11778 putative VrrB (vrrB) gene, partial cds. ... Nucleotide (GenBank) : U02893 Bacillus cereus ATCC 11778 16S rRNA gene, partial sequence. ...
... cereus sensu lato group, since SigB has been demonstrated to support pathogenesis in Bacillus, Listeria and Staphylococcus ... cereus, B. anthracis, B. mycoides, B. pseudomycoides, B. thuringiensis, B. weihenstephanensis and B. cytotoxicus) that recent ... The Bacillus cereus sensu lato group currently includes seven species (B. ... Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. J ...
Linking Bacillus cereus genotypes and carbohydrate utilization capacity. Alicja K. Warda, Roland J. Siezen, Jos Boekhorst, ... Linking Bacillus cereus genotypes and carbohydrate utilization capacity. In: PLoS ONE. 2016 ; Vol. 11, No. 6. ... Linking Bacillus cereus genotypes and carbohydrate utilization capacity. / Warda, Alicja K.; Siezen, Roland J.; Boekhorst, Jos ... title = "Linking Bacillus cereus genotypes and carbohydrate utilization capacity",. abstract = "We characterised carbohydrate ...
The first gene of the hbl operon, hblC, in the B. cereus type strain, ATCC 14579, was inactivated in this study. Inactivation ... HBL is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of B. cereus. The ... of hblC strongly reduced both the enterotoxigenic activity of B. cereus ATCC 14579 and the haemolytic activity against sheep ... is a Bacillus cereus toxin composed of a binding component, B, and two lytic components, L1 and L2. ...
Bacillus cereus from the Bacillus cereus group species, which consist of: Bacillus cereus, Bacillus thuringiensis, Bacillus ... The design and analysis of unique DNA primer sets and probes to identify and distinguish the bacillus cereus group species: ... The design and analysis of unique DNA primer sets and probes to identify and distinguish the bacillus cereus group species: ... anthracis, Bacillus weihenstephanensis, Bacillus mycoides and Bacillus pseudomycoides is one of the most frequently isolated ...
Bacilli. › Bacillales. › Bacillaceae. › Bacillus. › Bacillus cereus group. › Bacillus cereus. Strains i. › AH820. ... Bacillus cereus AH820. ›Bacillus cereus str. AH820. ›Bacillus cereus strain AH820. Rank i. - ...
Organism: BACILLUS CEREUS B4264 If you have problems or comments... Back to PBIL home page ... Organism: BACILLUS CEREUS B4264 6. BACC4_1_PE1002 SubName: Full=Sulfate/thiosulfate import ATP-binding protein CysA; EC=3.6.3 ... Organism: BACILLUS CEREUS B4264 14. BACC4_1_PE101 RecName: Full=Elongation factor G; Short=EF-G; (BACC4_1.PE101). Keywords: ... Organism: BACILLUS CEREUS B4264 3. BACC4_1_PE100 RecName: Full=30S ribosomal protein S7; (BACC4_1.PE100). Keywords: 30S ...
Emetic food poisoning caused by Bacillus cereus.. Holmes JR, Plunkett T, Pate P, Roper WL, Alexander WJ. ... incriminated macaroni and cheese as a cause of the illness and samples of this food contained large numbers of Bacillus cereus ... Previous outbreaks of B cereus emetic food poisoning have been associated with consumption of contaminated fried rice and may ...
Bacillus cereus prosthetic valve endocarditis.. Castedo E1, Castro A, Martin P, Roda J, Montero CG. ... Bacillus cereus is a ubiquitous organism that often contaminates microbiological cultures but rarely causes serious infections ... Reports of B. cereus endocarditis are infrequent. Infection in patients with valvular heart disease is associated with ... We describe a case of B. cereus endocarditis involving a mechanical mitral prosthesis that resolved after replacement of the ...
Study looks at rate of Bacillus cereus in infant formula in China. By News Desk on November 8, 2018. ... Australian scientists discover how to fight Bacillus cereus bacteria. By News Desk on December 18, 2018. ... Researchers at the Australian National University (ANU) have uncovered how Bacillus cereus works and how to combat it. ... Researchers have investigated the prevalence of Bacillus cereus in infant formula sold in China. ...
As a result of contamination with spore containing soil particles or dust, B. cereus can be easily transmitted to foods. ... cereus is a spore forming bacterium which is found worldwide. ... Bacillus cereus. Bacillus (B.) cereus is a spore forming ... Initial B. cereus contaminations on foods are mostly low. Usually, cell growth to bacterial counts of 105 to 108 colony-forming ... Moreover, B. cereus is capable of forming biofilms, which also enables the organism to persist in food producing environments. ...
Bacillus cereus bacteremia in an adult with acute leukemia.. Funada H1, Uotani C, Machi T, Matsuda T, Nonomura A. ... Bacillus cereus, which used to be considered non-pathogenic, was isolated from the blood of a patient with acute leukemia who ... Thus, this case suggested some new important clinicopathologic features of true B. cereus bacteremia complicating acute ...
Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose.. Mikami, B., Adachi, M., Kage, T., ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ... These results indicate that the ability of B. cereus beta-amylase to digest raw starch can be attributed to the additional two ...
BACILLUS CEREUS IN A MATERNITY UNIT = INFECTION À BACILLUS CEREUS DANS UN SERVICE DE MATERNITÉ  World Health Organization (‎ ... BACILLUS CEREUS FOOD POISONING = INTOXICATIONS ALIMENTAIRES À BACILLUS CEREUS  World Health Organization (‎1973)‎ ... FOODBORNE DISEASES : Bacillus cereus = MALADIES DORIGINE ALIMENTAIRE : Bacillus cereus  World Health Organization (‎1986)‎ ... 1973)‎. BACILLUS CEREUS FOOD POISONING = INTOXICATIONS ALIMENTAIRES À BACILLUS CEREUS. Weekly Epidemiological Record = Relevé ...
Campaña contra Bacillus Cereus. Ana Sofía González Alvaro Mendoza. Sintomatología. No crece por debajo de 4,4 ºC y se destruye ... B. cereus puede producir dos enterotoxinas: la toxina diarreica y la toxina emética. La intoxicación alimentaria por Bacillus ... Bacillus cereus causa intoxicaciones alimentarias a través de la ingesta de alimentos contaminados.. ¿Qué es?. Temperaturas ... Transcript of Bacillus Cereus. Es un bacilo formador de esporas responsable de intoxicaciones alimentarias, siendo su hábitat ...
... : The Bacillus cereus group comprises six members: Bacillus anthracis, Bacillus cereus, Bacillus mycoides, ... A review of Bacillus cereus. Bacillus cereus. Adapted from Per Einar Granum in Foodborne Pathogens: Microbiology and Molecular ... The availability of such foods could increase the prominence of Bacillus cereus as a foodborne pathogen. Bacillus cereus ... Bacillus pseudomycoides, Bacillus thuringiensis and Bacillus weihenstephanensis. These species are closely related and should ...
Bacillus cereus ATCC ® 12826™ Designation: Type Strain A, variant IV TypeStrain=False Application: Sporicidal test ... The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973. Loessner MJ, et al. Three Bacillus cereus ... Bacillus cereus Frankland and Frankland (ATCC® 12826™) Strain Designations: Type Strain A, variant IV / Type Strain: no / ... Zwittermicin A-producing strains of Bacillus cereus from diverse soils. Appl. Environ. Microbiol. 60: 4404-4412, 1994. PubMed: ...
Bacillus cereus ATCC ® 21366™ Designation: TypeStrain=False Application: Produces an enzyme preparation lytic on yeast cell ... Bacillus cereus Frankland and Frankland (ATCC® 21366™) Type Strain: no / Biosafety Level: 1 ...
Bacillus cereus ATCC ® 7064™ Designation: NRS 201 TypeStrain=False Application: Bacteriophage host Does not produce ... Bacillus cereus Frankland and Frankland (ATCC® 7064™) Strain Designations: NRS 201 [ATCC 12137, ATCC 8470] / Type Strain: no / ... Zwittermicin A-producing strains of Bacillus cereus from diverse soils. Appl. Environ. Microbiol. 60: 4404-4412, 1994. PubMed: ... Nucleotide (GenBank) : X53507 Bacillus cereus gene for oligo-1,6-glucosidase (EC 3.2.1.10). ...
cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Bacillaceae › BacillusBacillus ... tr,Q7BQ69,Q7BQ69_BACCE ZmaL OS=Bacillus cereus OX=1396 GN=zmaL PE=4 SV=2 ...
The Bacillus cereus group comprises seven closely related species: B. cereus sensu stricto (referred to herein as B. cereus), B ... Bacillus cereus and other members of Bacillus are not easily killed by alcohol; in fact, they have been known to colonize ... "Notes from the Field: Contamination of Alcohol Prep Pads with Bacillus cereus Group and Bacillus Species --- Colorado, 2010". ... McKillip JL (2000). "Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp., a literature review ...
Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application: ... Bacillus cereus Frankland and Frankland (ATCC® 10987D-5™) Strain Designations: Genomic DNA from Bacillus cereus strain NRS 248 ... Bacillus cereus Frankland and Frankland ATCC® 10987D-5™ dried Total DNA: At least 5 µg in 1X TE buffer ... Genomic DNA from Bacillus cereus strain NRS 248 [ATCC® 10987™] Biosafety Level 1 Biosafety classification is based on U.S. ...
Genomic DNA from Bacillus cereus strain FDA 5 TypeStrain=False Application: ... Bacillus cereus Frankland and Frankland (ATCC® 10702D-5™) Strain Designations: Genomic DNA from Bacillus cereus strain FDA 5 [ ... Bacillus cereus Frankland and Frankland ATCC® 10702D-5™ frozen Total DNA: At least 5 µg in 1X TE buffer ... Genomic DNA from Bacillus cereus strain FDA 5 [ATCC® 10702™] Biosafety Level 1 Biosafety classification is based on U.S. Public ...
Gemonic DNA from Bacillus cereus strain PCI 246 TypeStrain=False Application: ... Bacillus cereus Frankland and Frankland (ATCC® 13061D-5™) Strain Designations: Gemonic DNA from Bacillus cereus strain PCI 246 ... Bacillus cereus Frankland and Frankland ATCC® 13061D-5™ frozen Total DNA: At least 5 µg in 1X TE buffer ... Gemonic DNA from Bacillus cereus strain PCI 246 [ATCC® 13061™] Biosafety Level 1 Biosafety classification is based on U.S. ...
Bacillus cereus Domain: Prokaryote Optimal Growth Medium: Nutrient Broth Optimal Growth Temperature: 30° C Package: Tube ... Biosafety Level: 1 Gram Stain: Gram-Positive Shape: Bacillus (rod-shaped) ... Genus and Species: Bacillus cereus. Domain: Prokaryote. Optimal Growth Medium: Nutrient Broth. Optimal Growth Temperature: 30° ... Bacillus cereus, Living, Nutrient Broth, Tube. Item # 154872 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
  • Growth of B. cereus results in production of several highly active toxins therefore, consumption of food containing 105-106 bacteria (spores)/g or toxins, is sufficient to cause emetic and diarrhoeal syndromes. (ul.ie)
  • The assays performed well with milk samples artificially contaminated with bacteria belonging to the B. cereus group spp. (ul.ie)
  • iv) In addition multiplex PCR with primers targeting motB and a hypothetical protein proved successful in identification of the B. cereus group spp. (ul.ie)
  • The solving of the crystal structure of the neutral sphingomyelinase from Listeria ivanovii and Bacillus cereus has allowed a fuller understanding of their enzymatic site. (wikipedia.org)
  • The solving of the crystal structure of the neutral sphingomyelinase from Listeria ivanovii and Bacillus cereus has also shed light on their catalytic mechanisms. (wikipedia.org)
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