A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.
A species of gram-positive bacteria that is a common soil and water saprophyte.
A species of bacteria that causes ANTHRAX in humans and animals.
Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.
A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth.
Infections with bacteria of the family BACILLACEAE.
The reproductive elements of lower organisms, such as BACTERIA; FUNGI; and cryptogamic plants.
A species of bacteria whose spores vary from round to elongate. It is a common soil saprophyte.
Proteins found in any species of bacterium.
Agents that cause vomiting. They may act directly on the gastrointestinal tract, bringing about emesis through local irritant effects, or indirectly, through their effects on the chemoreceptor trigger zone in the postremal area near the medulla.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
An enzyme that catalyzes the endohydrolysis of 1,6-alpha-glucosidic linkages in isomaltose and dextrins produced from starch and glycogen by ALPHA-AMYLASES. EC 3.2.1.10.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
The functional hereditary units of BACTERIA.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A beta-lactamase preferentially cleaving penicillins. (Dorland, 28th ed) EC 3.5.2.-.
An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.
Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.
Compounds consisting of chains of AMINO ACIDS alternating with CARBOXYLIC ACIDS via ester and amide linkages. They are commonly cyclized.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A class of enzymes that catalyze the hydrolysis of phosphoglycerides or glycerophosphatidates. EC 3.1.-.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
An enzyme that catalyzes the hydrolysis of sphingomyelin to ceramide (N-acylsphingosine) plus choline phosphate. A defect in this enzyme leads to NIEMANN-PICK DISEASE. EC 3.1.4.12.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
A purine nucleoside that has hypoxanthine linked by the N9 nitrogen to the C1 carbon of ribose. It is an intermediate in the degradation of purines and purine nucleosides to uric acid and in pathways of purine salvage. It also occurs in the anticodon of certain transfer RNA molecules. (Dorland, 28th ed)
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
An enzyme that catalyzes the hydrolysis of 1,4-alpha-glycosidic linkages in starch, glycogen, and related polysaccharides and oligosaccharides so as to remove successive beta-maltose units from the non-reducing ends of the chains. EC 3.2.1.2.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method.
Substances that reduce the growth or reproduction of BACTERIA.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
Acute suppurative inflammation of the inner eye with necrosis of the sclera (and sometimes the cornea) and extension of the inflammation into the orbit. Pain may be severe and the globe may rupture. In endophthalmitis the globe does not rupture.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The relationships of groups of organisms as reflected by their genetic makeup.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A TETRACYCLINE analog having a 7-chloro and a 6-methyl. Because it is excreted more slowly than TETRACYCLINE, it maintains effective blood levels for longer periods of time.
A TETRACYCLINE with a 7-chloro substitution.
A TETRACYCLINE analog isolated from the actinomycete STREPTOMYCES rimosus and used in a wide variety of clinical conditions.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
An actinomycete from which the antibiotic CHLORTETRACYCLINE is obtained.

Utilization of exogenous purine compounds in Bacillus cereus. Translocation of the ribose moiety of inosine. (1/1270)

Intact cells of Bacillus cereus catalyze the breakdown of exogenous AMP to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. Inosine hydrolase was not detectable, even in crude extracts. Inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. Even though the equilibrium of the phosphorolytic reaction favors nucleoside synthesis, exogenous inosine (as well as adenosine and AMP) is almost quantitatively transformed into external hypoxanthine, since ribose 1-phosphate is readily metabolized inside the cell. Most likely, the translocated ribose 1-phosphate enters the sugar phosphate shunt, via its prior conversion into ribose 5-phosphate, thus supplying the energy required for the subsequent uptake of hypoxanthine in B. cereus.  (+info)

A randomly amplified polymorphic DNA marker specific for the Bacillus cereus group is diagnostic for Bacillus anthracis. (2/1270)

Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment with AluI distinguished B. anthracis from the other species of the B. cereus group.  (+info)

Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4. (3/1270)

The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.  (+info)

Evaluation of accuracy and repeatability of identification of food-borne pathogens by automated bacterial identification systems. (4/1270)

The performances of five automated microbial identification systems, relative to that of a reference identification system, for their ability to accurately and repeatedly identify six common food-borne pathogens were assessed. The systems assessed were the MicroLog system (Biolog Inc., Hayward, Calif.), the Microbial Identification System (MIS; MIDI Inc., Newark, Del.), the VITEK system (bioMerieux Vitek, Hazelwood, Mo.), the MicroScan WalkAway 40 system (Dade-MicroScan International, West Sacramento, Calif.), and the Replianalyzer system (Oxoid Inc., Nepean, Ontario, Canada). The sensitivities and specificities of these systems for the identification of food-borne isolates of Bacillus cereus, Campylobacter jejuni, Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., and verotoxigenic Escherichia coli were determined with 40 reference positive isolates and 40 reference negative isolates for each pathogen. The sensitivities of these systems for the identification of these pathogens ranged from 42.5 to 100%, and the specificities of these systems for the identification of these pathogens ranged from 32.5 to 100%. Some of the systems had difficulty correctly identifying the reference isolates when the results were compared to those from the reference identification tests. The sensitivity of MIS for the identification of S. aureus, B. cereus, E. coli, and C. jejuni, for example, ranged from 47.5 to 72. 5%. The sensitivity of the Microlog system for the identification of E. coli was 72.5%, and the sensitivity of the VITEK system for the identification of B. cereus was 42.5%. The specificities of four of the five systems for the identification of all of the species tested with the available databases were greater than or equal to 97.5%; the exception was MIS for the identification of C. jejuni, which displayed a specificity of 32.5% when it was tested with reference negative isolates including Campylobacter coli and other Campylobacter species. All systems had >80% sensitivities for the identification of Salmonella species and Listeria species at the genus level. The repeatability of these systems for the identification of test isolates ranged from 30 to 100%. Not all systems included all six pathogens in their databases; thus, some species could not be tested with all systems. The choice of automated microbial identification system for the identification of a food-borne pathogen would depend on the availability of identification libraries within the systems and the performance of the systems for the identification of the pathogen.  (+info)

Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice. (5/1270)

As 16S rRNA sequence analysis has proven inadequate for the differentiation of Bacillus cereus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic marker. The gyrB genes of B. cereus JCM 2152(T), Bacillus thuringiensis IAM 12077(T), Bacillus mycoides ATCC 6462(T), and Bacillus anthracis Pasteur #2H were cloned and sequenced. Oligonucleotide PCR primer sets were designed from within gyrB sequences of the respective bacteria for the specific amplification and differentiation of B. cereus, B. thuringiensis, and B. anthracis. The results from the amplification of gyrB sequences correlated well with results obtained with the 16S rDNA-based hybridization study but not with the results of their phenotypic characterization. Some of the reference strains of both B. cereus (three serovars) and B. thuringiensis (two serovars) were not positive in PCR amplification assays with gyrB primers. However, complete sequencing of 1.2-kb gyrB fragments of these reference strains showed that these serovars had, in fact, lower homology than their originally designated species. We developed and tested a procedure for the specific detection of the target organism in boiled rice that entailed 15 h of preenrichment followed by PCR amplification of the B. cereus-specific fragment. This method enabled us to detect an initial inoculum of 0.24 CFU of B. cereus cells per g of boiled rice food homogenate without extracting DNA. However, a simple two-step filtration step is required to remove PCR inhibitory substances.  (+info)

Semiautomated metabolic staining assay for Bacillus cereus emetic toxin. (6/1270)

This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.  (+info)

Effect of hydrostatic tensile stress on the growth of Escherichia coli and Bacillus cereus. (7/1270)

The specific growth rates of Escherichia coli and Bacillus cereus were measured for growth media in a flask, a lens-plate arrangement simulating an isolated capillary space, and a lens-plate arrangement under hydrostatic tensile stress. The specific growth rates of the bacteria were the same for the flask and lens-plate arrangement without hydrostatic tensile stress, but were enhanced when the growth media were subjected to hydrostatic tensile stress. The enhanced specific growth rates reached steady values at a tensile stress of 40 pascals. The effect was observed up to tensile stresses of around 100 pascals. The maximum increase in specific growth rate was 25% for E. coli and 22% for B. cereus.  (+info)

Isolation of stable ribosomal subunits from spores of Bacillus cereus. (8/1270)

Analyses of ribosomes extracted from spores of Bacillus cereus T by a dryspore disruption technique indicated that previously reported defects in ribosomes from spores may arise during the ribosome extraction process. The population of ribosomes from spores is shown to cotain a variable quantity of free 50S subunits which are unstable, giving rise to slowly sedimenting particles in low-Mg2+ sucrose gradients and showing extremely low activity in in vitro protein synthesis. The majority of the ribosomal subunits in spores, obtained by dissociation of 70S ribosomes and polysomes, are shown to be as stable as subunits from vegetative cells, though the activity of spore polysomes was lower than that of vegetative ribosomes. In spite of the instability and inactivity of a fraction of the spore's ribosomal subunits, the activity of the total population obtained from spores by the dry disruption technique was 32% of vegetative ribosome activity, fivefold higher than previously obtained with this species. The improvement in activity and the observed variability of subunit destabilization are taken as evidence for partial degradation of spore ribosomes during extraction.  (+info)

The Bacillus cereus sensu lato group currently includes seven species (B. cereus, B. anthracis, B. mycoides, B. pseudomycoides, B. thuringiensis, B. weihenstephanensis and B. cytotoxicus) that recent phylogenetic and phylogenomic analyses suggest are likely a single species, despite their varied phenotypes. Although horizontal gene transfer and insertion-deletion events are clearly important for promoting divergence among these genomes, recent studies have demonstrated that a major basis for phenotypic diversity in these organisms may be differential regulation of the highly similar gene content shared by these organisms. To explore this hypothesis, we used an in silico approach to evaluate the relationship of pathogenic potential and the divergence of the SigB-dependent general stress response within the B. cereus sensu lato group, since SigB has been demonstrated to support pathogenesis in Bacillus, Listeria and Staphylococcus species. During the divergence of these organisms from a common
Introduction. Bacillus cereus is a gram positive, facultatively anaerobic, spore-forming rod widely distributed environmentally, with no substantial pathogenic potential despite its great capacity of surviving adverse conditions1. This microorganism is frequently associated with gastrointestinal infections, mostly related to rice ingestion, and is characterized by vomiting and diarrhea in the immunocompetent host1,3. It belongs to the genus Bacillus that comprises seven closely related species: Bacillus cereus sensu lato,Bacillus anthracis,Bacillus thuringiensis, Bacillus mycoides, Bacillus pseudomycoides, Bacillus weihenstephanensis and Bacillus cytotoxicus. These strains share a highly conserved genome, and their 16S rRNA gene sequences show high levels of similarity2. Extraintestinal infections due to B. cereus are rare; however, when present they could manifest as bacteremia, endocarditis, meningitis, endophthalmitis, pneumonia, and soft tissue infections3. Therefore, the isolation of this ...
Bacillus cereus from the Bacillus cereus group species, which consist of: Bacillus cereus, Bacillus thuringiensis, Bacillus anthracis, Bacillus weihenstephanensis, Bacillus mycoides and Bacillus pseudomycoides is one of the most frequently isolated bacterial foodborne pathogens. Growth of B. cereus results in production of several highly active toxins therefore, consumption of food containing 105-106 bacteria (spores)/g or toxins, is sufficient to cause emetic and diarrhoeal syndromes. The most common source of this bacterium is milk and mixed food products that include milk powder, thus is of particular concern in the baby formula industry. In this study 138 strains of B. cereus group spp. were characterized based on their phenotypic and genotypic features. The study developed unique DNA primers for use in PCR and these were then tested via real-time PCR (RT-PCR): (i) the motB gene encoding the flagellar motor protein MotB was used as a PCR primer target. (ii) New primers and probes, targeting ...
TY - JOUR. T1 - Linking Bacillus cereus genotypes and carbohydrate utilization capacity. AU - Warda, Alicja K.. AU - Siezen, Roland J.. AU - Boekhorst, Jos. AU - Wells-Bennik, Marjon H.J.. AU - de Jong, Anne. AU - Kuipers, Oscar P.. AU - Nierop Groot, Masja N.. AU - Abee, Tjakko. PY - 2016. Y1 - 2016. N2 - We characterised carbohydrate utilisation of 20 newly sequenced Bacillus cereus strains isolated from food products and food processing environments and two laboratory strains, B. cereus ATCC 10987 and B. cereus ATCC 14579. Subsequently, genome sequences of these strains were analysed together with 11 additional B. cereus reference genomes to provide an overview of the different types of carbohydrate transporters and utilization systems found in B. cereus strains. The combined application of API tests, defined growth media experiments and comparative genomics enabled us to link the carbohydrate utilisation capacity of 22 B. cereus strains with their genome content and in some cases to the panC ...
Bacillus thuringiensis is widely used as a bioinsecticide due to its ability to form parasporal crystals containing proteinaceous toxins. It is a member of the Bacillus cereus sensu lato, a group with low genetic diversity but produces several promising antimicrobial compounds. B. thuringiensis DNG9, isolated from an oil-contaminated slough in Algeria, has strong antibacterial, antifungal and biosurfactant properties. Here, we report the 6.06 Mbp draft genome sequence of B. thuringiensis DNG9. The genome encodes several gene inventories for the biosynthesis of bioactive compounds such as zwittermycin A, petrobactin, insecticidal toxins, polyhydroxyalkanoates and multiple bacteriocins. We expect the genome information of strain DNG9 will provide another model system to study pathogenicity against insect pests, plant diseases, and antimicrobial compound mining and comparative phylogenesis among the Bacillus cereus sensu lato group.
Haemolysin BL (HBL) is a Bacillus cereus toxin composed of a binding component, B, and two lytic components, L1 and L2. HBL is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of B. cereus. The three genes encoding the HBL components constitute an operon and are transcribed from a promoter 608 bp upstream of the hblC translational start site. The first gene of the hbl operon, hblC, in the B. cereus type strain, ATCC 14579, was inactivated in this study. Inactivation of hblC strongly reduced both the enterotoxigenic activity of B. cereus ATCC 14579 and the haemolytic activity against sheep erythrocytes, while maintaining full haemolytic activity against human erythrocytes.
Bacillus cereus ATCC ® 10987D-5™ Designation: Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application:
Bacillus cereus ATCC ® 10987D-5™ Designation: Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application:
The Bacillus cereus sensu lato group consists of six species (B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis, and B. weihenstephanensis). While classical microbial taxonomy proposed these organisms as distinct species, newer molecular phylogenies and comparative genome sequencing suggests that these organisms should be classified as a single species (thus, we will refer to these organisms collectively as the Bc species-group). How do we account for the underlying similarity of these phenotypically diverse microbes? It has been established for some time that the most rapidly evolving and evolutionarily flexible portions of the bacterial genome are regulatory sequences and transcriptional networks. Other studies have suggested that the sigma factor gene family of these organisms has diverged and expanded significantly relative to their ancestors; sigma factors are those portions of the bacterial transcriptional apparatus that control RNA polymerase recognition for promoter
Abstract: Antimicrobial food packaging is vital issue. This study aimed to test shelf life extension of Cheddar Processed Cheese at room temperature using low-density polyethylene (LDPE) films incorporating nisin against Bacillus cereus. In particular, the efficiency of different treatments was tested against Bacillus cereus. Different concentrations (0, 500, 1000, 2000, 4000, 8000, 16000 IU mL-1) of nisin were used in activating (coating) LDPE films. There was no mainly clear difference in inhibition zone from 2000 to 16000 IU, so that a concentration of 2000 IU was used for active packaging. Four different treatments were tested in cheese: samples without coating; samples without coating and inculcated by Bacillus cereus; samples coating by polyethylene films that activated by nisin without inoculation and samples coating by the same films and inoculated by Bacillus cereus. Microbiological analyses including total count, spore forming bacteria and yeast and moulds of the cheese samples were ...
Bacillus cereus is a Gram positive rod-shaped aerobic, endospore-forming bacterium. Strains of B. cereus are widely distributed in the environment, mainly in soil, from where they easily spread to many types of foods, especially of vegetable origin, as well as meat, eggs, milk, and dairy products. This bacterium is one of the leading causes of food poisoning in the developed world. B. cereus causes two types of food-borne intoxications. One type is characterized by nausea and vomiting and abdominal cramps and has an incubation period of 1 to 6 hours. This is the short-incubation or emetic form of the disease. The second type is manifested primarily by abdominal cramps and diarrhea with an incubation period of 8 to 16 hours. This type is referred to as the long-incubation or diarrheal form of the disease [1, 2].. Different strategies may be employed to prevent B. cereus poisoning, like heating food above 75°C before use to kill vegetative cells. However, increasing trends for use of packed ...
The Bacillus cereus group of bacteria comprises soil-dwelling saprophytes but on occasion these bacteria can cause a wide range of diseases in humans, including food poisoning, systemic infections and highly lethal forms of anthrax. While anthrax is almost invariably caused by strains from a single evolutionary lineage, Bacillus anthracis, variation in the virulence properties of strains from other lineages has not been fully addressed. Using multi-locus sequence data from 667 strains, we reconstructed the evolutionary history of the B. cereus group in terms of both clonal inheritance and recombination. The strains included 155 clinical isolates representing B. anthracis, and isolates from emetic and diarrhoeal food poisoning, septicaemia and related infections, wound, and lung infections. We confirmed the existence of three major clades and found that clinical isolates of B. cereus (with the exception of emetic toxin-producing strains) are evenly distributed between and within clades 1 and 2. ...
Net weight: 130 grams. A sample of the above product, collected at a supermarket in Yuen Long, was found to be contaminated with Bacillus cereus last week. During follow-up investigation on the same day, the CFS collected another sample from the same batch of the product from another supermarket in Yuen Long for testing. The test result showed that the sample contained Bacillus cereus at a level of 200,000 per gram, a CFS spokesman said.. Upon notification of the contamination of the affected batch of bottled preserved bean curd with Bacillus cereus last week, the Centre urged the public and the trade not to eat or sell the product in question, and contacted the importer/distributor concerned immediately to trace the distribution of the food item concerned and instructed it to recall the affected batch of the product. CFS staff also conducted inspections at local retail outlets afterwards and no affected product was found available for sale.. According to the Microbiological Guidelines for ...
Discrimination by automatic ribotyping and random amplified polymorphic DNA PCR, RAPD, was compared for 40 different B. cereus dairy isolates, 4 different B. mycoides isolates and 6 culture collection strains. RAPD-PCR has previously shown to be useful for tracing contamination routes of B. cereus to milk. Automatic ribotyping using EcoRI and PvuII separated the B. cereus and B. mycoides isolates/strains into 36 different ribotypes. RAPD-typing with primers generated 40 different RAPD-profiles. However, 17 isolates clustered into eight groups, irrespective of the primer and restriction enzyme used, and in all but one case, the isolates with the same pattern were isolated from the same dairy. Automatic ribotyping proved to be a useful, standardized and quick method to discriminate between B. cereus strains, only slightly less discriminatory than RAPD-typing.. ...
Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.
Bacillus cereus is widespread in nature and frequently isolated from soil and growing plants . In addition to rice, pasta, and spices, dairy products are among the most common food vehicles for B. cereus. There are two types of B. cereus foodborne illness. The first type, which is caused by an emetic toxin, results in vomiting, whereas the second type, which is caused by enterotoxin(s), results in diarrhea. Little is known about susceptible populations, but the more severe types of the illness have occasionally involved young athletes (19 years) or the elderly (60 years). The emetic toxin, causing vomiting, had been isolated and characterized, whereas the diarrheal disease is caused by one or more enterotoxins. The spore of B. cereus is an important factor in foodborne illness. First, the B. cereus spore is more hydrophobic than any other Bacillus spp. spores, which enables it to adhere to several types of surfaces. Neither of the two commercial immunoassays available for enterotoxin detection can
SUMMARY: Growth of an oligosporogenic strain of Bacillus cereus T was studied in continuous culture. The maximum specific growth rate obtained was 0.47 h−1. RNA content increased linearly with increasing growth rate. Protein content decreased at high growth rates, but the DNA content of the culture was not affected by the growth rate. Activities of enzymes involved in glutamate utilization and energy production increased with increasing growth rate. The maximum specific activity for all enzymes assayed was obtained at a dilution rate of about 0.30 h−1. Changing the concentration of zinc or manganese in the medium by as little as 10−7 M from the concentration optimum for growth caused a significant reduction in the steady-state bacterial population. Zinc appeared to affect the efficiency of conversion of substrate to biomass. Manganese was very toxic at concentrations slightly above the concentration optimum for a specific growth rate.
Abstract: Bacterial strains isolated from the gut of the local termite Coptotermes curvignathus were inoculated into a buffered medium containing minerals and Whatman filter paper as the sole carbon source to observe the ability of the bacteria to digest solid substrate. The bacteria were Bacillus cereus strain Razmin A, Enterobacter aerogenes strain Razmin B, Enterobacter cloacae strain Razmin C, Acinetobacter strain Raminalimon and Chryseobacterium kwangyangense strain Cb. The Gen Bank NCBI/EMBL accession numbers for the bacterial strains were EU294508, EU305608, EU305609, EU332791 and EU169201, respectively. The ability of bacterial cultures to grow in this medium as well as to digest the filter paper was determined by visual observation after 30 days. All bacterial cultures showed growth as the medium turned cloudy and the filter paper became macerated. Chryseobacterium kwangyangense strain Cb showed yellow pigmented colonies on the filter paper. Bacillus cereus strain Razmin A showed clumps ...
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
Three double-stranded DNA phi29-like Bacillus cereus group bacteriophages, BeachBum, Harambe, and SerPounce, were identified and characterized. BeachBum and Harambe are closely related but are remarkably different from previously identified phi29-like phages. SerPounce is substantially closer to other phi29-like phages, enabling the identification of its prohead RNA (pRNA) gene ...
PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. part 2 Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples
Using state-of-the-art genome sequence-based phylogenomic methods, we elucidated the taxonomy of the important Bacillus cereus group. The article is published under an open access license in Scientific Reports.. ...
Bacillus cereus strain BP01 is a microbial plant regulator that is used on cotton to produce higher quality cotton at lower cost. ...
|i|Bacillus cereus|/i| is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections. The authors report three cases of |i|B cereus|/i| bacteremia among injection drug users presenting within one month to an urban tertiary care hospital. Treatment with intravenous vancomycin was successful in all three cases. While temporal association suggested an outbreak, molecular studies of patient isolates using pulsed-field gel electrophoresis did not suggest a common source. A review of the association of |i|B cereus|/i| infections with heroin use and treatment of this pathogen is provided.
Food-borne intoxications are increasingly caused by the dodecadepsipeptide cereulide (L-O-Val-L-Val-D-O-Leu-D-Ala)3, the emetic toxin produced by Bacillus cereus (Fig. 1A/C). As such intoxications pose a health risk to humans, a comprehensive understanding on the chemodiversity of this toxin is mandatory for the reliable risk assessment of B. cereus toxins in foods. A fast and robust high-throughput UPLC-ToF-MS profiling method was developed and successfully applied to discriminate a total of 78 Bacillus cereus strains into no (group 1, Fig. 1A), low/medium (group 2/3) and high (group 4/5) producers of the emetic toxin cereulide. Mass spectrometric screening and multivariate analysis (Fig. 1B) showed a series of at least 18 cereulide variants, among which the previously unknown isocereulides A-G (Fig. 1C) were determined for the first time by means of UPLC-ToF-MS and ion-trap MSn sequencing, 13C-labelling experiments, and post-hydrolytic dipeptide and enantioselective amino acid analysis. Most ...
Other names: ATCC 14579, B. cereus, BCRC 10603, Bacillus cereus, Bacillus endorhythmos, Bacillus medusa, Bacillus sp. 2479, Bacillus sp. BS2(2013b), Bacillus sp. BV4, Bacillus sp. JKR50, Bacillus sp. JKR62, Bacillus sp. JP44SK22, Bacillus sp. JP44SK37, Bacillus sp. JP44SK43, Bacillus sp. JP44SK45, Bacillus sp. JSG1(2014), Bacillus sp. KER 17, Bacillus sp. MZ-01, Bacillus sp. PXDK-1, Bacillus sp. Pf-1, Bacillus sp. V3, Bacillus sp. mmm86, CCM 2010, CCRC 10603, CCUG 7414, CIP 66.24, DSM 31, IAM 12605, IFO 15305, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504 ...
Other names: ATCC 14579, B. cereus, BCRC 10603, Bacillus cereus, Bacillus endorhythmos, Bacillus medusa, Bacillus sp. 2479, Bacillus sp. BS2(2013b), Bacillus sp. BV4, Bacillus sp. JKR50, Bacillus sp. JKR62, Bacillus sp. JP44SK22, Bacillus sp. JP44SK37, Bacillus sp. JP44SK43, Bacillus sp. JP44SK45, Bacillus sp. JSG1(2014), Bacillus sp. KER 17, Bacillus sp. MZ-01, Bacillus sp. PXDK-1, Bacillus sp. Pf-1, Bacillus sp. V3, Bacillus sp. mmm86, CCM 2010, CCRC 10603, CCUG 7414, CIP 66.24, DSM 31, IAM 12605, IFO 15305, JCM 2152, LMG 6923, NBRC 15305, NCCB 75008, NCIMB 9373, NCTC 2599, NRRL B-3711, VKM B-504 ...
TY - JOUR. T1 - Complete genome sequence of Bacillus cereus NC7401, which produces high levels of the emetic toxin cereulide. AU - Takeno, Akira. AU - Okamoto, Akira. AU - Tori, Keizo. AU - Oshima, Kenshiro. AU - Hirakawa, Hideki. AU - Toh, Hidehiro. AU - Agata, Norio. AU - Yamada, Keiko. AU - Ogasawara, Naotake. AU - Hayashi, Tetsuya. AU - Shimizu, Tohru. AU - Kuhara, Satoru. AU - Hattori, Masahira. AU - Ohta, Michio. PY - 2012/9. Y1 - 2012/9. N2 - We report the complete and annotated genome sequence of Bacillus cereus NC7401, a representative of the strain group that causes emetic-type food poisoning. The emetic toxin, cereulide, is produced by a nonribosomal protein synthesis (NRPS) system that is encoded by a gene cluster on a large resident plasmid, pNCcld.. AB - We report the complete and annotated genome sequence of Bacillus cereus NC7401, a representative of the strain group that causes emetic-type food poisoning. The emetic toxin, cereulide, is produced by a nonribosomal protein ...
Bacillus cereus food poisoning has been implicated in outbreak episodes in the world. Bacillus cereus is a potential food safety hazard causesing two different types of food poisoning, the diarrhea type, caused by hemolysin BL and non-hemolytic enterotoxin, and the emetic type, caused by the emetic toxin ccreulide. In Kenya the organism has been isolated from pasteurized milk at 41.2%, 26% of which were enterotoxigenic. The aim of this study was to, develop a multiplex polymerase chain reation (PCR) technique that can be used to detect enterotoxigenic B. cereus in food, determine the level of contamination in various foods that will enable gene detection, and to develop suitable food processing procedures that can reduce PCR inhibitory substances to enable use of direct multiplex PCR for detection of toxins genes in various foods. In this study, seven pairs of primers for multiplex PCR were used for simulteneous detection of 6 genes and a specific sequence encording hemolysin BL, non-hemolytic ...
Fingerprint Dive into the research topics of Extracellular virulence factors in Bacillus cereus endophthalmitis: Methods and implication of involvement of hemolysin BL. Together they form a unique fingerprint. ...
Bacillus cereus was reported to occur in high numbers (up to 107 CFU/g) during spontaneous fermentation of three different traditional Benin condiments; afitin, iru and sonru made from African locust beans. A total of nineteen B. cereus isolates from the ferments, were examined for the presence of toxin genes related to food poisoning using PCR. Isolates harboring the emetic specific gene fragment EM1 where highly representative, being one out of ten isolates from afitin, one of one isolate from iru and five of eight isolates from sonru. Three of the isolates from afitin harbored the cytotoxin K gene. The non hemolytic enterotoxin complex genes were present in all isolates, while the hemolytic enterotoxin complex genes were present in only one isolate from afitin. Food poisoning potential was evaluated using a Vero-cell assay measuring cytotoxity, and the toxin titers ranged between 80 and 1280. Isolates from afitin were the most cytotoxic. The emetic isolates from afitin, iru and sonru were low ...
The present study was performed on a studfarm under typical field conditions. To minimize effects related to husbandry, feeding, and season, our study took place at one thoroughbred farm, within one foaling period from February to May. According to Lahrssen and Zentek [18] such a study design is important when working with a limited number of animals. Probiotic dosing and treatment period were in accordance with studies by Jeroch et al. [19], Jadamus et al. [20], and Vilà et al. [15].. Unfortunately some foals were treated with antibiotics during a severe period of illness which may have an impact on the subsequent microbial profile in faeces.. Bacterial microflora in faeces was used as an indication of the effects of B. cereus var. toyoi on the intestinal health of foals. Although faeces might have some limitations for describing the gut ecosystem, by comparing microflora and the digestion process in the colon with faeces in fistulated horses Julliand and Goachet [21] showed that the faecal ...
Abstract:. Bacillus cereus typically presents as a gastrointestinal infection, but rarely manifests as systemic disease. This report describes a case of Bacillus cereus related endocarditis that presented as a sickle cell crisis and bacteremia. Initial clinical suspicion was for laboratory contamination of blood cultures. The case herein described is intended to demonstrate an uncommon presentation of Bacillus cereus infection and highlights the value of an aggressive need to further investigate and interpret unexpected blood culture findings in clinical practice, early adequate antimicrobial therapy, prompt diagnosis, and consideration to urgent surgical interventions in such cases.. ...
Bacillus mycoides isolate Bac J, a non-pathogenic, phyllosphere-inhabiting biological control agent, reduced Cercospora leaf spot of sugar beet by 60-80% in glasshouse experiments, even when spatially separated from the causal agent, Cercospora beticola Sacc. Disease control was attributed to the ability of the bacterium to induce systemic resistance in the host, which was demonstrated through classical induced resistance challenge assays. Additionally, in glasshouse and field experiments three pathogenesis-related proteins, chitinase, beta-glucanase and peroxidase, that are accepted molecular markers of systemic induced resistance, were increased by nearly 2-fold in distal, untreated sugar beet leaves following treatment with Bacillus mycoides isolate Bac J and acibenzolar-S-methyl, a chemical inducer of systemic resistance. The increased activity in all cases was a result of the production of unique isoforms of the enzymes not found in the water treated control. The Bacillus mycoides isolate ...
The genome of Bacillus cereus contains 26 Nudix hydrolase genes, second only to its closest relative, Bacillus anthracis which has 30. All 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. Substrates for 16 of the enzymes were identified; these included ADP-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphosphates. One of the enzymes was a CDP-choline pyrophosphatase, the first Nudix hydrolase active on this substrate. Furthermore, as a result of this and previous work we have identified a new sub-family of the Nudix hydrolase superfamily recognizable by a specific amino acid motif outside of the Nudix box.
4 edition of A survey of four study areas examining Bacillus cereus population distributions and soil metal concentrations found in the catalog.. ...
TY - JOUR. T1 - Recovery of Bacillus cereus cyclodextrin glycosyltransferase and recycling of phase components in an aqueous two-phase system using thermo-separating polymer. AU - Ng, Hui Suan. AU - Tan, Chin Ping. AU - Mokhtar, Mohd Noriznan Mohd. AU - Ibrahim, Shaliza. AU - Ariff, Arbakariya B. AU - Ooi, Chien Wei. AU - Ling, Tau Chuan. PY - 2012. Y1 - 2012. UR - http://dx.doi.org/10.1016/j.seppur.2011.12.028. U2 - 10.1016/j.seppur.2011.12.028. DO - 10.1016/j.seppur.2011.12.028. M3 - Article. VL - 89. SP - 9. EP - 15. JO - Separation and Purification Technology. JF - Separation and Purification Technology. SN - 1383-5866. ER - ...
1J18: Crystal Structure of a Catalytic Site Mutant of beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltopentaose
1J18: Crystal Structure of a Catalytic Site Mutant of beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltopentaose
Food Poisoning AgentVWR offers slides for the varied purposes of your lab. Prepared and digital microscope slides for educational purposes are featured in an array of fields. General purpose microscope slides and cover glasses are offered as well as cavity, chamber, adhesion, and microarray slides for more specific research needs. Disposable and reusable options of varying thickness and diameter are available and may be purchased in kits, packs, cases, or individually.
Introduction: B. anthracis is a highly fatal infectious agent in animals and humans. In this era of bioterrorism, the risk of exposing a large population to this lethal pathogen has increased dramatically. Therefore, its early and accurate diagnostic detection is essential for successful treatment and control of spread. Due to the isolation of very closely related Bacillus cereus group species, definitive molecular identification of Bacillus anthracis needs detection of specific markers for at least three different loci, one chromosome and two virulence plasmids (pXO1, and pXO2). It is difficult to find a chromosome-specific marker due to the genetic similarity among the B. cereus group species. There are several reports of the use of a B. anthraics chromosome-specific marker, but there has not been a marker which was extensively tested in vitro with many strains of the B. cereus group species, including the closely related ones to B. anthracis. In addition, most of currently reported multiplex ...
Using an insect model to assess correlation between temperature and virulence in Bacillus weihenstephanensis and Bacillus cereus ...
Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization o …
Kinetics of Indigenous Isolated Bacteria Bacillus mycoides Used for Ex-Situ Bioremediation of Petroleum Contaminated Soil in PT Pertamina Sungai Lilin South Sumatera
This recall has been initiated because a surface in a manufacturing area was found to be contaminated with the bacterium Bacillus cereus, an organism commonly found in food and soil. No contamination of the ingredients or in the vaccine itself was found and all the batches passed quality and sterility testing before release in Australia.. There is no concern regarding the safety for patients. Those who have received vaccination from the affected batches do not require revaccination.. Please inspect your stocks and quarantine all units from the above batch numbers for return to your supplier who will arrange for stock to be replaced or refunded. Any items from these batches supplied by HPS Pharmacies should be returned to the pharmacy for processing. If any stock has been transferred to other units, please forward this notice on to them.. No other batches of INFANRIX hexa Vaccine or any other GSK vaccines are affected by this recall. Other batches are available and no supply problems are ...
On September 14, 2016, CDC published an Interim Final Rule adding B. cereus biovar anthracis to the list of HHS select agents and toxins to be regulated as Tier 1 agent. The regulation was published in the Federal Register and CDC will be accepting public comment on the addition of the agent for the next 60 days.. B. cereus biovar anthracis is an emerging pathogen that has properties very similar to Bacillus anthracis (which is currently regulated as a Tier 1 agent). The agent has been isolated from great apes with anthrax-like disease in several countries in Africa. This Interim Final Rule amends the HHS list of select agents and toxins​, while simultaneously undertaking a public comment period. Given the potential for safety and security concerns due to its similarities to Bacillus anthracis, CDC believes that it is important to regulate this agent now while it appears to be limited in distribution.. The Federal Experts Security Advisory Panel (FESAP) also provided policy and technical input ...
Previous DNA relatedness studies showed that strains idnentified as Bacillus mycoides segregated into two genetically distinct yet phenotypically similar groups, one being B. mycoides sensu stricto and the other, an unclassified taxon. In the present study, the taxonomic position of this second group was assessed by measuring DNA relatedness and determining phenotypic characteristics of an increased number of B. mycoides strains. Also determined was the second group's 16S RNA gene sequence. The 36 B. mycoides strains studied segregated into two genetically distinct groups showing DNA relatedness of about 30%; 18 strains represented the species proper and 18 the second group with intragroup DNA relatedness for both groups ranging from 70 to 100%. DNA relatedness to the type strains of presently recognized species with G+C contents of approximately 35 mol% (Bacillus alcalophilus, Bacillus cereus, Bacillus circulans, Bacillus lentus, Bacillus megaterium and Bacillus sphaericus) ranged from 22 to 37%.
Bacillus cereus, an infectious cause of foodborne illness, accounted for 2% of outbreaks with confirmed etiology that were reported to CDC during 1973-1987 (1). On July 21, 1993, the Lord Fairfax (Virginia) Health District received reports of acute gastrointestinal illness that occurred among children and staff at two jointly owned child day care centers following a catered lunch. This report summarizes the investigation of this outbreak. The catered lunch was served on July 21 to 82 children aged less than or equal to 6 years and to nine staff; dietary histories were obtained for 80 persons. Staff and all children aged greater than or equal to 4 years were interviewed directly; staff and parents were questioned for children aged less than 4 years. Of the 80 persons, 67 ate the catered lunch. A case was defined as vomiting by a person who was present at either day care center on July 21. Fourteen (21%) persons who ate the lunch became ill, compared with none of 13 who did not. Symptoms included ...
The substrate-induced inactivation of beta-lactamase I from Bacillus cereus 569/H has been studied. Both the wild-type enzyme and mutants have been used. The kinetics follow a branched pathway of the type recently analysed [Waley (1991) Biochem. J. 279, 87-94]. The substrate cloxacillin (a penicillin) formed an acyl-enzyme (characterized by m.s.), and it was probably the instability of this intermediate that brought about inactivation. A disulphide bond was introduced into beta-lactamase I (the wild-type enzyme lacks this bond) by site-directed mutagenesis: Ala-77 and Ala-123 were replaced by cysteine. Spontaneous oxidation yielded the disulphide. The activity of this newly cross-linked enzyme was a little diminished, but the stability towards inactivation by cloxacillin was not increased. A second mutant of beta-lactamase I was studied: this mutant lacked the first 17 residues, i.e. the first alpha-helix. The mutant had reduced activity towards ordinary (non-inactivating) substrates and no ...
Bacillus anthracis je povzročitelj antraksa - pogoste bolezni živine in občasno ljudi - in edini obligatni patogen iz rodu Bacillus.[1] Gre za grampozitivno, endosporogeno, paličasto bakterijo s širino od 1 do 1,2 mikrometra in dolžino od 3 do 5 µm.[1] Raste lahko v običajnem hranilnem mediju v aerobnih ali anaerobnih razmerah.[2] Bacillus anthracis je ena od maloštevilnih vrst bakterij, ki sintetizirajo polipeptidno kapsulo (poli-D-gama-glutamat). Tako kot Bordetella pertussis tudi ta vrsta tvori od kalmodulina odvisni adenilat-ciklazni eksotoksin, imenovan edemski faktor. Genotipsko in fenotipsko je podobna vrstama Bacillus cereus in Bacillus thuringiensis. Vse tri vrste imajo podobno celično velikost in obliko. Vse tvorijo ovalne spore, ki ležijo centralno v nenabreklem sporangiju. Spore B. anthracis so izjemno odporne in preživijo več desetletij ali stoletij kljub ekstremnim temperaturam, pomanjkanju hranil ali intenzivnim kemičnim dejavnikom. ...
Isolated from chimpanzees that had died with clinical symptoms of anthrax in Tai National Park, Cote dIvoire (CI) in 2001 and 2002 ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|163941511|ref|YP_001646395.1| from Bacillus weihenstephanensis KBAB4. Plus protein sequence and external database links.
Seattle/King County Public Health is investigating an outbreak of gastroenteritis with abdominal cramps and diarrhea associated with Rancho Bravo Tacos at
Al-Kaabi, Nasser; A. Al-Ghouti, Mohammad; Oualha, Meriam; Yousaf Mohammad, Mohammad; Al-Naemi, Aziza; I. Sølling, Theis; Al-Shamari, Noora; Zouari, Nabil... more authors ... less authors ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group; Bacillus ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group; Bacillus ...
phdthesis{1903540, abstract = {Raw cows milk is a product of high nutritional value but this automatically implies it is a medium highly suitable for growth of spoilage organisms that negatively affect milk quality and safety through production of proteolytic and lipolytic enzymes and toxins. Two major groups of spoilage bacteria are recognized, namely pseudomonads and bacilli. Identification of members of these groups is hampered by their confusing taxonomic situation. Both taxa historically grew as dumping grounds for aerobic Gram-positive spore-forming rods in the case of bacilli, and aerobic Gram-negative rods in the case of pseudomonads, resulting in two very large, heterogeneous groups. As a consequence, members of these groups were often poorly identified based on identification tools with insufficient resolution, and usually only two major species were recognized in the issue of milk spoilage, namely Bacillus cereus and Pseudomonas fluorescens. Nonetheless, several studies on the ...
This disposable bacteria filter attaches between your CPAP machine and your CPAP tubing to provide post-machine bacteria filtering.
This work represents an interdisciplinary effort to investigate microbiological and chemical manganese (Mn) cycling in drinking water systems using concepts and tools from civil and environmental engineering, microbiology, chemistry, surface science, geology, and applied physics. Microorganisms were isolated from four geographically diverse drinking water systems using selective Mn-oxidizing and -reducing culture media. 16S rRNA gene sequencing revealed that most are bacteria of the Bacillus spp. (i.e., Bacillus pumilus and Bacillus cereus). These bacteria are capable of performing Mn-oxidation and -reduction under controlled laboratory conditions. Pseudo-first order rate constants obtained for microbiological Mn-oxidation and -reduction (aerobic and anaerobic) of these isolates ranged from 0.02 - 0.66 days^-1. It is likely that spores formed by Bacillus spp. protect them from chlorine and other disinfectants applied in drinking water systems, explaining their ubiquitous presence. A new method ...
Several related concepts make use of similar words, and the ambiguity can create considerable confusion. The term Bacillus (capitalized and italicized) is also the name of a genus that, among many other genera, falls within the class Bacilli.. Also, bacillus (or the plural bacilli) can be a generic term to describe the morphology of any rod-shaped bacterium. This general term does not mean that the subject is a member of class Bacilli or genus Bacillus. Thus, it does not necessarily imply a similar group of characteristics. Not all members of class Bacilli are rod-shaped (Staphylococcus is spherical), and many other rod-shaped bacteria that do not fall within that class (Clostridium kamina dalla kotta is rod-shaped but very different taxonomically) exist. Moreover, the general term bacillus does not necessarily indicate the Gram-positive staining common to class Bacilli. For example, E. coli is a rod-shaped bacterium that could, therefore, be described as a bacillus, but it stains ...
Twenty-five strains were isolated on Aleksandrov medium from sample soils/weathered rocks of Ha Tien Mountain, Kien Giang, Vietnam. Their colonies were round or irregular, white to yellow and their shape was rod, motile. Seventeen of twenty-five bacterial strains were identified by PCR technique with specific primers fd1 and rP, they were capable of dissolving both phosphate and potassium and seven strains had high phosphate and potassium dissolution capacity (|10 mg l-1 P2O5 and |50 mg l-1 K2O) effectively. These seven strains were chosen to sequence, DNA sequencing were compared with GenBank database of NCBI by BLAST N software. The results showed that DNV16 strain was similarity of 99% with EU977655.1 (Microbacterium hominis), TC1D strain was a 99% similarity with FN547417.1 (Flectobacillus sp.), CH9E strain was a 99% similarity of 99% with GQ861463.1 (Agrobacterium tumefasciens), TC1A strain was similarity of 99% with HM003210.1 (Bacillus cereus) and FJ976533.1 (Bacillus coagulans), CH7A strain was
Saccharides have a central role in the nutrition of all living organisms. Whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. This multi-component system includes an integral membrane protein EIIC that transports saccharides and assists in their phosphorylation. Here we present the crystal structure of an EIIC from Bacillus cereus that transports diacetylchitobiose. The EIIC is a homodimer, with an expansive interface formed between the amino-terminal halves of the two protomers. The carboxy-terminal half of each protomer has a large binding pocket that contains a diacetylchitobiose, which is occluded from both sides of the membrane with its site of phosphorylation near the conserved His250 and Glu334 residues. The structure shows the architecture of this important class of transporters, identifies the determinants of substrate binding and ...
TY - JOUR. T1 - Crystal structure of a phosphorylation-coupled saccharide transporter. AU - Cao, Yu. AU - Jin, Xiangshu. AU - Levin, Elena J.. AU - Huang, Hua. AU - Zong, Yinong. AU - Quick, Matthias. AU - Weng, Jun. AU - Pan, Yaping. AU - Love, James. AU - Punta, Marco. AU - Rost, Burkhard. AU - Hendrickson, Wayne A.. AU - Javitch, Jonathan A.. AU - Rajashankar, Kanagalaghatta R.. AU - Zhou, Ming. PY - 2011/5/5. Y1 - 2011/5/5. N2 - Saccharides have a central role in the nutrition of all living organisms. Whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. This multi-component system includes an integral membrane protein EIIC that transports saccharides and assists in their phosphorylation. Here we present the crystal structure of an EIIC from Bacillus cereus that transports diacetylchitobiose. The EIIC is a homodimer, with an expansive interface formed ...
RN [1] RL Curr Biol. 1995 Dec 1;5(12):1424-36. RT Extent and character of circadian gene expression in Drosophila melanogaster: identification of twenty oscillating mRNAs in the fly head. RA Van Gelder RN, Bae H, Palazzolo MJ, Krasnow MA. RM PMID: 8749395 RN [2] RM PMID: 7966317 RT Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search. RA Koonin EV, Tatusov RL. RL J Mol Biol 1994 Nov 18;244(1):125-32 RN [2] RM PMID: 11601995 RT MDP-1 is a new and distinct member of the haloacid dehalogenase family of aspartate-dependent phosphohydrolases. RA Selengut, JD RL Biochemistry 2001 Oct 23;40(42):12704-11 RN [3] RM PMID: 10956028 RT The crystal structure of bacillus cereus phosphonoacetaldehyde hydrolase: insight into catalysis of phosphorus bond cleavage and catalytic diversification wi thin the HAD enzyme superfamily. RA Morais MC, Zhang W, Baker AS, Zhang G, Dunaway-Mariano D, ...
We continue to innovate, growing our assay repertoire and capabilities - allowing you to perform all required assays on raw ingredients, finished products, as well as test environmental samples and processing water. With automated monitoring of ready-to-use assay vials and automated data processing and archiving, the microbiologists job got a lot easier with the same accurate results in less than half the time. Our innovative vial design prevents product interference even when directly testing products such as yogurt and salad dressing. Come to our booth (#400) or contact us directly (734-984-3100) to learn more about the exciting new developments we are featuring at this years IAFP: our Bacillus cereus assay, Heterotrophic Bacteria in water along with our environmental monitoring solutions. The BioLumix Advantage. • Vial design prevents product interference. • Automation and connectivity allows faster product release. • Real-time communication for immediate action. • Expedited ...
Potassium channel molecule. Computer model showing the structure of a bacterial potassium channel with four units arranged around an axis going through the first (and only visible) of four potassium ions (big purple sphere). From Bacillus cereus. - Stock Image C035/8257
ID A0A0G1K391_9BACT Unreviewed; 426 AA. AC A0A0G1K391; DT 22-JUL-2015, integrated into UniProtKB/TrEMBL. DT 22-JUL-2015, sequence version 1. DT 25-OCT-2017, entry version 16. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,RuleBase:RU000577, ECO:0000256,SAAS:SAAS00724181}; DE Flags: Fragment; GN ORFNames=UW75_C0051G0005 {ECO:0000313,EMBL:KKT78008.1}; OS Parcubacteria group bacterium GW2011_GWF2_44_8. OC Bacteria; unclassified Parcubacteria group. OX NCBI_TaxID=1618971 {ECO:0000313,EMBL:KKT78008.1, ECO:0000313,Proteomes:UP000034896}; RN [1] {ECO:0000313,EMBL:KKT78008.1, ECO:0000313,Proteomes:UP000034896} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A., RA Wilkins M.J., Williams K.H., Banfield J.F.; RT rRNA introns, odd ribosomes, and small enigmatic genomes across a RT large radiation of phyla.; RL Nature 0:0-0(2015). CC -!- FUNCTION: Plays an important role in the initiation and regulation ...
bacillus carrier Chinese translation: 杆菌载体。.... Please click for detailed translation, meaning, pronunciation and example sentences for bacillus carrier in Chinese
"The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral ... Bacillolysin (EC 3.4.24.28, Bacillus metalloendopeptidase, Bacillus subtilis neutral proteinase, anilozyme P 10, Bacillus ... Feder J, Keay L, Garrett LR, Cirulis N, Moseley MH, Wildi BS (October 1971). "Bacillus cereus neutral protease". Biochimica et ... "Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but ...
Bacillus cereus targets milk and cream. To counter this, we commonly have to store food in cool place like inside a ... Loss of Soil fertility:- Anaerobic bacteria such as Bacillus denitrificans reduce the nitrates of the poorly aerated soil to ...
Sanwal BD, Zink MW (1961). "L-Leucine dehydrogenase of Bacillus cereus". Arch. Biochem. Biophys. 94 (3): 430-435. doi:10.1016/ ...
Olsen DB, Hepburn TW, Moos M, Mariano PS, Dunaway-Mariano D (1988). "Investigation of the Bacillus cereus phosphonoacetaldehyde ... identification of 2-phosphonoacetaldehyde as an intermediate in the degradation of 2-aminoethylphosphonate by Bacillus cereus ...
Kellenberger, G.; Kellenberger, E. (1952-01-01). "[Bacteriolysis of a strain of bacillus cereus; evidence in electronic ...
The soil bacterium Bacillus cereus can be used to transform resveratrol into piceid (resveratrol 3-O-beta-D-glucoside). Only a ... "Biotransformation of resveratrol to piceid by Bacillus cereus". J. Nat. Prod. 61 (10): 1313-4. doi:10.1021/np980139b. PMID ...
Kong, M; Kim, M; Ryu, S (June 2012). "Complete Genome Sequence of Bacillus cereus Bacteriophage PBC1". Journal of Virology. 86 ... Bacteriophage PBC1 is an unclassified member of the family that infects the spore-forming bacterium Bacillus cereus. Safferman ...
... is a bacteriophage that infects the spore-forming bacterium Bacillus cereus. Though this lytic phage is a ... Kong, M; Kim, M; Ryu, S (June 2012). "Complete Genome Sequence of Bacillus cereus Bacteriophage PBC1". Journal of Virology. 86 ...
Zinc-metallophospholipases C: Clostridium perfringens alpha-toxin, Bacillus cereus PLC (BC-PLC) Sphingomyelinases: B. cereus, ... The genes encoding alpha-toxin (Clostridium perfringens), Bacillus cereus PLC (BC-PLC), and PLCs from Clostridium bifermentans ... "Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus" (PDF). Product Information. Sigma Aldrich. Hilgemann DW ( ... "The metal ion dependence of phospholipase C from Bacillus cereus". Biochimica et Biophysica Acta (BBA) - Enzymology. 391 (2): ...
A number of pure cultures, e.g. Trichosporon cutaneum, Bacillus cereus, Klebsiella oxytoca, Pseudomonas sp. etc. individually, ...
... is an antibiotic that has been identified from the bacterium Bacillus cereus UW85. It is a molecule of interest ... Emmert EA, Klimowicz AK, Thomas MG, Handelsman J (January 2004). "Genetics of zwittermicin a production by Bacillus cereus". ... Haiyin, He (April, 1994) "Zwittermicin A, an Antifungal and Plant Protection Agent from Bacillus cereus", Tetrahedron Letters ... April 2007). "Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki ...
"Antimicrobial activity of carvacrol toward Bacillus cereus on rice". Journal of Food Protection. 63 (5): 620-624. doi:10.4315/ ...
Bacillus cereus strain AIMST Nalbe1 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Nalbe7 16S ... Bacillus cereus strain AIMST Nalbe8 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Nalme2 16S ... Bacillus cereus strain AIMST Nalme13 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Ngme4 16S ... Bacillus cereus strain AIMST Ngse7 16S ribosomal RNA gene, partial sequence. NCBI. Bacillus cereus strain AIMST Ngse9 16S ...
Oyama, T.; Kusunoki, M.; Kishimoto, Y.; Takasaki, Y.; Nitta, Y. (1999). "Crystal structure of beta-amylase from Bacillus cereus ... "The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251". The Journal of ...
Examples of bacterial species that can form endospores include Bacillus cereus, Bacillus anthracis, Bacillus thuringiensis, ... Bacteria having a centrally placed endospore include Bacillus cereus. Sometimes the endospore can be so large the cell can be ... In Bacillus subtilus endospores, the spore coat is estimated to contain more than 70 coat proteins, which are organized into an ... Bacillus subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of ...
"A pseudo-tRNA modulates antibiotic resistance in Bacillus cereus". PLOS ONE. 7 (7): e41248. Bibcode:2012PLoSO...741248R. doi: ...
... the emetic toxin of Bacillus Cereus". Takabe F, Oya M (1976). "An autopsy case of food poisoning associated with Bacillus ... Certain strains of Bacillus cereus - a common species of bacterium implicated as a frequent cause of food poisoning - can cause ... 1997). "Fulminant liver failure in association with the emetic toxin of Bacillus cereus". N Engl J Med. 336 (16): 1142-1148. ... 2011). "Sudden Death of a Young Adult Associated with Bacillus cereus Food Poisoning". J Clin Microbiol. 49 (12): 4379-4381. ...
Little C, Otnåss AB (June 1975). "The metal ion dependence of phospholipase C from Bacillus cereus". Biochimica et Biophysica ...
In Bacillus cereus, cereulide biosynthesis occurs by the non-ribosomal peptide synthetase of the heterodimer proteins CesA and ... Cereulide is a toxin produced by some strains of Bacillus cereus. It is a potent cytotoxin that destroys mitochondria. It also ... News on cereulide, the emetic toxin of Bacillus Cereus M. A. Andersson; R. Mikkola; J. Helin; M. C. Andersson; M. Salkinoja- ... Agata N, Ohta M, Mori M, Isobe M (1995). "A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus". FEMS ...
"Antimicrobial activity of a newly identified bacteriocin of Bacillus cereus". Applied and Environmental Microbiology. 59 (12): ...
Bacillus cereus spores were killed at four minutes of microwaving. A 2017 study was less affirmative: about 60% of the germs ...
MCM-like helicase in Bacillus cereus strain ATCC 14579 (BcMCM; Q81EV1) is an SF6 helicase fused with an AEP primase. The enzyme ...
... s are a group of bacteriocins produced by various strains of the bacterium Bacillus cereus. Although all cereins are by ... Bacillus cereus MRX1". J Appl Microbiol. 103 (5): 1621-31. doi:10.1111/j.1365-2672.2007.03395.x. PMID 17953573. "CDC - Signs & ... a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity". FEMS Microbiol. Lett. 178 (2): 337-341. doi: ... a novel bacteriocin produced by Bacillus cereus Bc7". FEMS Microbiol. Lett. 254 (1): 108-15. doi:10.1111/j.1574-6968.2005.00009 ...
"The Arthromitus stage of Bacillus cereus: Intestinal symbionts of animals". Proceedings of the National Academy of Sciences of ... at isolating Arthromitus from termite guts led to the conclusion that Arthromitus is in fact a growth stage of Bacillus cereus ...
Budzik JM, Oh SY, Schneewind O (May 2009). "Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus ... Budzik JM, Marraffini LA, Schneewind O (October 2007). "Assembly of pili on the surface of Bacillus cereus vegetative cells". ... Budzik JM, Oh SY, Schneewind O (December 2008). "Cell wall anchor structure of BcpA pili in Bacillus anthracis". The Journal of ... "Amide bonds assemble pili on the surface of bacilli". Proceedings of the National Academy of Sciences of the United States of ...
... particularly Bacillus cereus and the anthrax-causing bacterium Bacillus anthracis. The exosporium is the portion of the spore ... anthracis and other members of the Bacillus cereus group. The protein ywdL has been identified in B. cereus as important for ... Terry, Cassandra; Shepherd, Andrew; Radford, David S.; Moir, Anne; Bullough, Per A. (2011). "YwdL in Bacillus cereus: Its Role ... In Bacillus anthraces, salt and detergent washing of exosporium fragments can identify proteins that are likely to represent ...
Cooked rice can contain Bacillus cereus spores, which produce an emetic toxin when left at 4-60 °C (39-140 °F). When storing ... One of the enterotoxins produced by Bacillus cereus is heat-resistant; reheating contaminated rice kills the bacteria, but does ...
Maruyama R, Nishizawa M, Itoi Y, Ito S, Inoue M (December 2001). "Isolation and expression of a Bacillus cereus gene encoding ...
It has also been isolated from Bacillus cereus associated with the entomopathogenic nematode Rhabditis (Oscheius) sp. and shown ... "Cyclic dipeptides from rhabditid entomopathogenic nematode-associated Bacillus cereus have antimicrobial activities". World ...
This is the case of Staphylococcus aureus and Bacillus cereus, for example. The concept of MID does not apply to them, but ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
Genotipsko in fenotipsko je podobna vrstama Bacillus cereus in Bacillus thuringiensis. Vse tri vrste imajo podobno celično ... Bacillus anthracis. Cohn 1872 Bacillus anthracis je povzročitelj antraksa - pogoste bolezni živine in občasno ljudi - in edini ... Wikimedijina zbirka ponuja več predstavnostnega gradiva o temi: Bacillus anthracis. *↑ 1,0 1,1 Spencer, Robert C. (marec 2003 ... Bacillus anthracis je ena od maloštevilnih vrst bakterij, ki sintetizirajo polipeptidno kapsulo (poli-D-gama-glutamat). Tako ...
Bacteria having a centrally placed endospore include Bacillus cereus. Sometimes the endospore can be so large the cell can be ... Kadota H, Iijima K (1965). "The X-ray diffraction pattern of spores of Bacillus subtilis". Agric Biol Chem. 29 (1): 80-81. doi: ... There is one report of viable spores of Bacillus marismortui in salt crystals approximately 250 million years old.[4][5] When ... In Bacillus subtilus endospores, the spore coat is estimated to contain more than 70 coat proteins, which are organized into an ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
Multiple bacilli (rod-shaped bacteria, here shown as black and bean-shaped) shown between white blood cells in urinary ...
Bacillus anthracis belongs to the Bacillus cereus group of strains.png,''Bacillus anthracis'' spada v skupino sevov ''Bacillus ... Bacillus anthracis (uredi) Redakcija: 00:53, 28. marec 2013 dodanih 89 zlogov , pred 7 leti ... Slika:B anthracis diagram en.png,Zgradba bakterije ''Bacillus anthracis''. Slika:AnthraxBacteria.jpg,Elektronska mikrografija ...
Bacillus brevis • Bacillus CereusBacillus megaterium • Bacillus subtilis • Burkholderia cepacia • Campylobacter jejuni • ...
Bacillus cereus which is responsible for a form of food poisoning.. *Bacillus thuringensis which is used in pest control. It ... Bacillus anthracis, which causes Anthrax.. *Bacillus subtilis is considered an model organism. It is often used in genetic ... Bacilli are partly or wholly aerobic. They do aerobic respiration. They are everywhere in nature. Bacillus includes both free- ... Bacillus is a genus of rod shaped bacteria. They are Gram-positive, meaning they have an extra outside cell layer. ...
Bacillus cereus bacterium chocolate [1] Bacillus coagulans bacterium chocolate [7] Bacillus licheniformis bacterium chocolate [ ... Bacillus stearothermophilus bacterium chocolate [1] Bacillus subtilis bacterium chocolate [1] Bacillus subtilis bacterium soy ...
B. cereus. B. chagannorensis. B. chitinolyticus. B. chondroitinus. B. choshinensis. B. chungangensis. B. cibi. B. circulans. B ... Bacillus adalah genus bakteri Gram-positif berbentuk batang yang anggota dari filum Firmicutes. Spesies Bacillus dapat bersifat ... Bacillus. Foto mikroskopis Bacillus subtilis dengan pewarnaan Gram dan pembesaran 1.000x. Bentuk oval yang tidak terwarnai ... Wikimedia Commons memiliki media mengenai Bacillus.. *Bacillus genomes and related information at PATRIC, a Bioinformatics ...
Other less common pathogens include Bacillus cereus, Clostridium perfringens, Clostridium difficile and Staphylococcus aureus.[ ...
Staphylococcus aureus and Streptococcus sanguinis and in gram-positive soil bacterium Bacillus subtilis, Bacillus cereus.[20] ... The non-spore formers are Corynebacterium and Listeria (a coccobacillus), whereas Bacillus and Clostridium produce spores.[16] ... Two of these, Streptococcus and Staphylococcus, are cocci (sphere-shaped). The remaining organisms are bacilli (rod-shaped) and ... Rod-shaped gram-positive Bacillus anthracis bacteria in a cerebrospinal fluid sample stand out from round white blood cells, ...
Wells, C. L., Wilkins, T. D. (1996). "Clostridia: Sporeforming Anaerobic Bacilli". In Baron, S.; et al. Baron's Medical ...
Bacillus cereus group: close and polyphyleticEdit. Main article: Bacillus cereus. In a similar way, the Bacillus species (= ... Main article: Bacillus. Another example of a large genus with nested genera is the genus Bacillus, in which the genera ... phylum Firmicutes) belonging to the "B. cereus group" (B. anthracis, B. cereus, B . thuringiensis, B. mycoides, B. ... and gyrB Gene Sequence Analysis to Determine Phylogenetic Relationships of Bacillus cereus Group Microorganisms". Journal of ...
"A Pseudo-tRNA Modulates Antibiotic Resistance in Bacillus cereus". PLoS ONE. 7 (7): e41248. Bibcode:2012PLoSO...741248R. doi: ...
Hämolysin aus Bacillus cereus,[16] Alveolysin aus Bacillus alvei, Streptolysin O aus Streptococcus pyogenes oder Tetanolysin ... N. Ramarao, V. Sanchis: The pore-forming haemolysins of bacillus cereus: a review. In: Toxins. Band 5, Nummer 6, Juni 2013, S. ... H. Lee, H. Y. Kim: Lantibiotics, class I bacteriocins from the genus Bacillus. In: Journal of microbiology and biotechnology. ...
Two Bacillus species are considered medically significant: B. anthracis, which causes anthrax, and B. cereus, which causes food ... B. cereus. B. chagannorensis. B. chitinolyticus. B. chondroitinus. B. choshinensis. B. chungangensis. B. cibi. B. circulans. B ... Bacillus is a genus of gram-positive, rod-shaped bacteria and a member of the phylum Firmicutes. Bacillus species can be ... Many Bacillus species are able to secrete large quantities of enzymes. Bacillus amyloliquefaciens is the source of a natural ...
Bacillus cereus infection. *Bacterial endocarditis. *Bacterial food poisoning. *Bacterial gastroenteritis. *Bacterial ...
Helgason, E., et al. (2000). Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis-One Species on the Basis of ... 7,0 7,1 Granum P.E. ja Lund, T. (1997). Bacillus cereus and its food poisoning toxins, FEMS, vol. 157(2), lk 223-228. ... Al-Khatib, M.S., et al. (2007). Incidence and characterization of diarrheal enterotoxins of fecal Bacillus cereus isolates ... 12,0 12,1 Pardo-Lopez, L., et al. (2013). Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect ...
Ivan C. Hall and Elizabeth O'Toole first named the bacterium Bacillus difficilis in 1935, choosing its specific epithet because ... Hall IC, O'Toole E (1935). "Intestinal flora in newborn infants with a description of a new pathogenic anaerobe, Bacillus ... "Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins". Microbiology ...
Bacteria having a centrally placed endospore include Bacillus cereus. Sometimes the endospore can be so large the cell can be ... There is one report of viable spores of Bacillus marismortui in salt crystals approximately 250 million years old.[4][5] When ... Bacillus subtilis spores are useful for the expression of recombinant proteins and in particular for the surface display of ... Endospores of the bacterium Bacillus anthracis were used in the 2001 anthrax attacks. The powder found in contaminated postal ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
At the microscopic level, a number of bacterial species (e.g. Bacillus subtilis, Streptococcus pneumoniae, Bacillus cereus) ...
... is a type of labile toxin found in Escherichia coli and Bacillus cereus. ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
Ia memiliki kemiripan genotipe dan fenotipe yang dekat dengan Bacillus cereus dan Bacillus thuringiensis. Ketiga spesies ... Bacillus cereus biovar anthracis, yaitu B. cereus dengan dua plasmid, juga mampu menyebabkan antraks. ... B. anthracis tergolong dalam kelompok B. cereus yang terdiri dari strain: B. cereus, B. anthracis, B. thuringiensis, B. ... Wikimedia Commons memiliki media mengenai Bacillus anthracis.. *Bacillus anthracis genomes and related information at PATRIC, a ...
Bacillus cereus infection Bacillus cereus Bacterial pneumonia multiple bacteria Bacterial vaginosis List of bacterial vaginosis ...
Bacillus. *Bacillus anthracis *Anthrax. *Bacillus cereus *Food poisoning. Listeria. *Listeria monocytogenes *Listeriosis ...
"Structure-activity relationships of Bacillus cereus and Bacillus anthracis dihydrofolate reductase: toward the identification ... Dihydrofolate reductase from Bacillus anthracis (BaDHFR) a validated drug target in the treatment of the infectious disease, ... Structural alignment of dihydrofolate reductase from Bacillus anthracis (BaDHFR), Staphylococcus aureus (SaDHFR), Escherichia ... "Targeted mutations of Bacillus anthracis dihydrofolate reductase condense complex structure−activity relationships". Journal ...
Isang Gram-positive Bacillus anthracis bacteria (sa lilang rods) sa halimbawang cerebrospinal fluid. Ang ibang mga selula ay ... Bacillus. Bacillus anthracis (Anthrax) · Bacillus cereus (Food poisoning). Listeria. Listeria monocytogenes (Listeriosis) ...
The Bacillus cereus group comprises seven closely related species: B. cereus sensu stricto (referred to herein as B. cereus), B ... B. cereus can parasitize codling moth larvae.[citation needed] B. cereus and other members of Bacillus are not easily killed by ... 2013). "Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated ... "Notes from the Field: Contamination of alcohol prep pads with Bacillus cereus group and Bacillus species - Colorado, 2010". CDC ...
Bacilli. › Bacillales. › Bacillaceae. › Bacillus. › Bacillus cereus group. › Bacillus cereus. Strains i. › AH820. ... Bacillus cereus AH820. ›Bacillus cereus str. AH820. ›Bacillus cereus strain AH820. Rank i. - ...
Organism: BACILLUS CEREUS B4264 If you have problems or comments... Back to PBIL home page ... Organism: BACILLUS CEREUS B4264 6. BACC4_1_PE1002 SubName: Full=Sulfate/thiosulfate import ATP-binding protein CysA; EC=3.6.3 ... Organism: BACILLUS CEREUS B4264 14. BACC4_1_PE101 RecName: Full=Elongation factor G; Short=EF-G; (BACC4_1.PE101). Keywords: ... Organism: BACILLUS CEREUS B4264 3. BACC4_1_PE100 RecName: Full=30S ribosomal protein S7; (BACC4_1.PE100). Keywords: 30S ...
Bacillus cereus biovar anthracis is a variant of the Bacillus cereus bacterium that has acquired plasmids similar to those of ... Bacillus cereus biovar anthracis infection has caused significant mortality in numerous mammalian species, including ... "Bacillus cereus Biovar Anthracis Causing Anthrax in Sub-Saharan Africa-Chromosomal Monophyly and Broad Geographic Distribution ... "The genome of a Bacillus isolate causing anthrax in chimpanzees combines chromosomal properties of B. cereus with B. anthracis ...
Emetic food poisoning caused by Bacillus cereus.. Holmes JR, Plunkett T, Pate P, Roper WL, Alexander WJ. ... incriminated macaroni and cheese as a cause of the illness and samples of this food contained large numbers of Bacillus cereus ... Previous outbreaks of B cereus emetic food poisoning have been associated with consumption of contaminated fried rice and may ...
Bacillus cereus prosthetic valve endocarditis.. Castedo E1, Castro A, Martin P, Roda J, Montero CG. ... Bacillus cereus is a ubiquitous organism that often contaminates microbiological cultures but rarely causes serious infections ... Reports of B. cereus endocarditis are infrequent. Infection in patients with valvular heart disease is associated with ... We describe a case of B. cereus endocarditis involving a mechanical mitral prosthesis that resolved after replacement of the ...
Bacillus cereus E33L. ›Bacillus cereus ZK. ›Bacillus cereus str. E33L. ›Bacillus cereus strain E33L. ... Bacilli. › Bacillales. › Bacillaceae. › Bacillus. › Bacillus cereus group. › Bacillus cereus. Strains i. › ZK / E33L. ...
Study looks at rate of Bacillus cereus in infant formula in China. By News Desk on November 8, 2018. ... Australian scientists discover how to fight Bacillus cereus bacteria. By News Desk on December 18, 2018. ... Researchers at the Australian National University (ANU) have uncovered how Bacillus cereus works and how to combat it. ... Researchers have investigated the prevalence of Bacillus cereus in infant formula sold in China. ...
As a result of contamination with spore containing soil particles or dust, B. cereus can be easily transmitted to foods. ... cereus is a spore forming bacterium which is found worldwide. ... Bacillus cereus. Bacillus (B.) cereus is a spore forming ... Initial B. cereus contaminations on foods are mostly low. Usually, cell growth to bacterial counts of 105 to 108 colony-forming ... Moreover, B. cereus is capable of forming biofilms, which also enables the organism to persist in food producing environments. ...
Structure of raw starch-digesting Bacillus cereus beta-amylase complexed with maltose.. Mikami, B., Adachi, M., Kage, T., ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ... The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b ... These results indicate that the ability of B. cereus beta-amylase to digest raw starch can be attributed to the additional two ...
BACILLUS CEREUS IN A MATERNITY UNIT = INFECTION À BACILLUS CEREUS DANS UN SERVICE DE MATERNITÉ  World Health Organization (‎ ... BACILLUS CEREUS FOOD POISONING = INTOXICATIONS ALIMENTAIRES À BACILLUS CEREUS  World Health Organization (‎1973)‎ ... FOODBORNE DISEASES : Bacillus cereus = MALADIES DORIGINE ALIMENTAIRE : Bacillus cereus  World Health Organization (‎1986)‎ ... 1973)‎. BACILLUS CEREUS FOOD POISONING = INTOXICATIONS ALIMENTAIRES À BACILLUS CEREUS. Weekly Epidemiological Record = Relevé ...
Campaña contra Bacillus Cereus. Ana Sofía González Alvaro Mendoza. Sintomatología. No crece por debajo de 4,4 ºC y se destruye ... B. cereus puede producir dos enterotoxinas: la toxina diarreica y la toxina emética. La intoxicación alimentaria por Bacillus ... Bacillus cereus causa intoxicaciones alimentarias a través de la ingesta de alimentos contaminados.. ¿Qué es?. Temperaturas ... Transcript of Bacillus Cereus. Es un bacilo formador de esporas responsable de intoxicaciones alimentarias, siendo su hábitat ...
... : The Bacillus cereus group comprises six members: Bacillus anthracis, Bacillus cereus, Bacillus mycoides, ... A review of Bacillus cereus. Bacillus cereus. Adapted from Per Einar Granum in Foodborne Pathogens: Microbiology and Molecular ... The availability of such foods could increase the prominence of Bacillus cereus as a foodborne pathogen. Bacillus cereus ... Bacillus pseudomycoides, Bacillus thuringiensis and Bacillus weihenstephanensis. These species are closely related and should ...
Bacillus cereus ATCC ® 12826™ Designation: Type Strain A, variant IV TypeStrain=False Application: Sporicidal test ... The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973. Loessner MJ, et al. Three Bacillus cereus ... Bacillus cereus Frankland and Frankland (ATCC® 12826™) Strain Designations: Type Strain A, variant IV / Type Strain: no / ... Zwittermicin A-producing strains of Bacillus cereus from diverse soils. Appl. Environ. Microbiol. 60: 4404-4412, 1994. PubMed: ...
Bacillus cereus ATCC ® 21366™ Designation: TypeStrain=False Application: Produces an enzyme preparation lytic on yeast cell ... Bacillus cereus Frankland and Frankland (ATCC® 21366™) Type Strain: no / Biosafety Level: 1 ...
Bacillus cereus ATCC ® 11778™ Designation: FDA strain PCI 213 TypeStrain=False Application: Assay of chlortetracycline ... Bacillus cereus Frankland and Frankland (ATCC® 11778™) Strain Designations: FDA strain PCI 213 [ATCC 9634, NCTC 10320, S.A. ... Nucleotide (GenBank) : AF238886 Bacillus cereus strain ATCC 11778 putative VrrB (vrrB) gene, partial cds. ... Nucleotide (GenBank) : U02893 Bacillus cereus ATCC 11778 16S rRNA gene, partial sequence. ...
The Bacillus cereus group, also known as B. cereus sensu lato, is a subdivision of the Bacillus genus that consists of eight ... Risks for public health related to the presence of Bacillus cereus and other Bacillus spp. including Bacillus thuringiensis in ... Bacillus thuringiensis strains display a similar repertoire of the potential virulence genes on the chromosome as B. cereus ... Bacillus cereus andB. thuringiensis strains are usually not discriminated in clinical diagnostics or food microbiology. Thus, ...
Bacillus cereus Domain: Prokaryote Optimal Growth Medium: Nutrient Broth Optimal Growth Temperature: 30° C Package: Tube ... Biosafety Level: 1 Gram Stain: Gram-Positive Shape: Bacillus (rod-shaped) ... Genus and Species: Bacillus cereus. Domain: Prokaryote. Optimal Growth Medium: Nutrient Broth. Optimal Growth Temperature: 30° ... Bacillus cereus, Living, Nutrient Broth, Tube. Item # 154872 *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
The authors report three cases of ,i,B cereus,/i, bacteremia among injection drug users presenting within one month to an urban ... A review of the association of ,i,B cereus,/i, infections with heroin use and treatment of this pathogen is provided. ... i,Bacillus cereus,/i, is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections. ... A Cluster of Bacillus cereus Bacteremia Cases among Injection Drug Users. Michael A Benusic,1 Natasha M Press. ,2 Linda MN ...
UPLC-TOF MS analysis of B. cereus strains in combination with 13C-labeling experiments now revealed tetra-, octa- and ... Cereulide and isocereulides A-G are biosynthesized as emetic toxins by Bacillus cereus via a non-ribosomal peptide synthetase ( ... A Bacillus cereus culture was incubated in the presence of 13C6-cereulide for up to 40 h and 13C6-cereulide as well as non- ... Stenfors Arnesen, L. P., Fagerlund, A., Granum, P. E. From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS ...
Food contaminated with Bacillus cereus may not look or smell spoiled.. The symptoms of Bacillus cereus food poisoning can ... on the Slim Fast recall because of possible Bacillus cereus contamination.. FDA says Bacillus cereus may cause diarrhea and ... Slim-Fast Recalled for Bacillus cereus. By Dan Flynn on December 5, 2009. ... ready-to drink meal replacement shake products described below because of the possible contamination with Bacillus cereus. ...
Seventy four Bacillus cereus outbreaks which resulted in 911 human cases over seven years in France underlines the need to ... Seventy four Bacillus cereus outbreaks which resulted in 911 human cases over seven years in France underlines the need to ... "Bacillus Cereus-induced foodborne outbreaks in France, 2007 to 2014: Epidemiology and genetic characterisation​" ... How common is foodborne B. cereus? ​. The study focused on 339 B. cereus strains isolated from food samples analysed during 74 ...
Peptidoglycan biosynthesis - Bacillus cereus B4264 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Sulfur metabolism - Bacillus cereus AH187 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , ...
SOURCE: Bacillus cereus ( ) Page printed: . Unofficial document if printed. Please refer to SOURCE for latest information. ... Bacillus cereus is a foodborne pathogen that can produce toxins, causing two types of gastrointestinal illness: the emetic ( ... As B. cereus is ubiquitous in the environment, control measures should be focused on preventing growth of B. cereus and the ... In Canada, over 36,000 cases of foodborne illness due to B. cereus were estimated to have occurred in 2006. B. cereus will grow ...
... Toshinobu Horii,1 Kiyoko Tamai,2 ... Toshinobu Horii, Kiyoko Tamai, Shigeyuki Notake, and Hideji Yanagisawa, "Bacillus cereus Bloodstream Infection in a Preterm ...
... you may have actually had a foodborne illness caused by the bacteria Bacillus cereus. Foods that have not been properly stored ... Bacillus cereus: A Foodborne Illness Confused with the 24-hour Flu. HYG-5576 ... The CDC estimates that there are over 63,000 cases of Bacillus cereus illness each year in this country, and that 100% of the ... The exact number of Bacillus cereus cases that occur each year is hard to determine because many people attribute their illness ...
Crystal structure of a conserved metalloprotein from Bacillus cereus.. Bonanno, J.B., Patskovsky, Y., Freeman, J., Bain, K.T., ... Crystal structure of a conserved metalloprotein from Bacillus cereus. *DOI: 10.2210/pdb3D19/pdb ...
Bacillus cereus food poisoning is the general description, although two recognized types of illness are caused by two distinct ... The symptoms of bacillus diarrheal type mimic those of clostridium perfringens. The onset of watery diarrhea, abdominal cramps ...
Data from analytical ultracentrifuge studies suggest that polymers of ribosomes exist in dormant spores of Bacillus cereus T. ...
  • Its virulence factors include cereolysin and phospholipase C.[citation needed] The Bacillus cereus group comprises seven closely related species: B. cereus sensu stricto (referred to herein as B. cereus), B. anthracis, B. thuringiensis, B. mycoides, B. pseudomycoides, and B. cytotoxicus. (wikipedia.org)
  • Bacillus cereus biovar anthracis is a variant of the Bacillus cereus bacterium that has acquired plasmids similar to those of Bacillus anthracis. (wikipedia.org)
  • Bacillus cereus biovar anthracis infection has caused significant mortality in numerous mammalian species, including chimpanzees. (wikipedia.org)
  • Bacillus anthracis , Bacillus cereus , Bacillus mycoides , Bacillus pseudomycoides , Bacillus thuringiensis and Bacillus weihenstephanensis . (highveld.com)
  • These species are closely related and should be placed within one species, except for Bacillus anthracis that possesses specific large virulence plasmids. (highveld.com)
  • B. cereus sensu stricto, B. anthracis , B. thuringiensis , B. weihenstephanensis , B. mycoides , B. pseudomycoides , B. cytotoxicus and B. toyonensis . (europa.eu)
  • Differentiation of these organisms depends upon determination of motility (most B. cereus are motile), presence of toxin crystals (B. thuringiensis), hemolytic activity (B. cereus and others are beta hemolytic whereas B. anthracis is usually nonhemolytic), and rhizoid growth which is characteristic of B. cereus var. (abcam.com)
  • Bacillus anthracis is the etiologic agent of anthrax, an acute fatal disease among mammals. (pnas.org)
  • Although initial phenotypic and 16S rRNA analysis identified this isolate as B. cereus , the rapid generation and analysis of a high-coverage draft genome sequence revealed the presence of a circular plasmid, named pBCXO1, with 99.6% similarity with the B. anthracis toxin-encoding plasmid, pXO1. (pnas.org)
  • Bacillus cereus and Bacillus anthracis are members of a closely related phylogenetic cluster referred to as the B. cereus group. (pnas.org)
  • Although classification of B. anthracis and B. cereus as separate species based on molecular analysis has been questioned, they differ in phenotype and in the diseases they cause ( 1 - 4 ). (pnas.org)
  • The assembly consisted of 131 contigs that were ordered and concatenated with our prior knowledge of the B. anthracis and B. cereus genome sequences ( 10 , 12 ). (pnas.org)
  • B. cereus was streaked onto trypticase soy agar containing 5% sheep blood (Becton Dickinson Microbiology Systems) and incubated in ambient atmosphere overnight at 37°C. B. anthracis was streaked onto trypticase soy agar containing 0.8% sodium bicarbonate and incubated in 5% CO 2 at 37°C. Cells from a single colony were dispersed into 5 μl of water on a microscope slide and covered with a coverslip. (pnas.org)
  • The presence of Si in bacterial spores (specifically, the spores of Bacillus anthracis ) again became the focus of attention when anthrax spores were mailed to U.S. senators in the fall of 2001 ( 17 ). (asm.org)
  • title=Cereulide synthetase gene cluster from emetic ''Bacillus cereus'': structure and location on a mega virulence plasmid related to ''Bacillus anthracis'' toxin plasmid pXO1. (wikidoc.org)
  • It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. (scirp.org)
  • The potential for horizontal transfer among the various subspecies of this group, which includes the human opportunistic pathogens B. cereus sensu stricto and B. anthracis as well as the biopesticide B. thuringiensis, has led to growing concerns regarding food safety and public health. (nih.gov)
  • The Bacillus genus includes B. anthracis, the causative agent of anthrax. (sciencephoto.com)
  • Vilas-Bôas GT, Peruca APS, Arantes OMN (2007) Biology and taxonomy of Bacillus cereus , Bacillus anthracis , and Bacillus thuringiensis Can. (springer.com)
  • Certain strains from the B. cereus group, which includes the opportunistic human pathogen B. cereus, insecticidal B. thuringiensis, and the obligate human and animal pathogen B. anthracis, are known to have the ability to form biofilm, an attached state in which cells are closely packed and firmly attached to each other and usually a solid surface. (uio.no)
  • We have also initiated gene disruption studies in a candidate regulatory gene, the pleiotropic transcriptional regulator plcR, to reveal its possible involvement in biofilm formation in Bacillus cereus ATCC 10987, a strain closely related to B. anthracis, isolated from spoiled cheese in the 1930s. (uio.no)
  • The Bacillus cereus sensu lato group currently includes seven species ( B. cereus , B. anthracis , B. mycoides , B. pseudomycoides , B. thuringiensis , B. weihenstephanensis and B. cytotoxicus ) that recent phylogenetic and phylogenomic analyses suggest are likely a single species, despite their varied phenotypes. (biomedcentral.com)
  • Through full genome analyses of four atypical Bacillus cereus isolates, designated B . cereus biovar anthracis, we describe a distinct clade within the B . cereus group that presents with anthrax-like disease, carrying virulence plasmids similar to those of classic Bacillus anthracis . (plos.org)
  • The isolates shared several phenotypic features of both B . anthracis and B . cereus , but differed amongst each other in motility and their resistance or sensitivity to penicillin. (plos.org)
  • Our findings show the existence of a discrete clade of the B . cereus group capable of causing anthrax-like disease, found in areas of high biodiversity, which are possibly also the origin of the worldwide distributed B . anthracis . (plos.org)
  • Anthrax has historically been attributed to a single cluster within the Bacillus cereus complex denoted as B . anthracis . (plos.org)
  • These strains, designated B . cereus biovar anthracis, combine bacteriological and molecular features of B . cereus and B . anthracis . (plos.org)
  • On the technical side it will be important to adapt diagnostic methods for the detection of such atypical B . cereus strains-through the inclusion of molecular tools for the detection of the B . anthracis virulence plasmids that appear to be the prerequisite to cause disease. (plos.org)
  • Bacillus anthracis has been classically defined as a clade with low genomic diversity within the B . cereus sensu lato group, whose members carry two virulence plasmids, pXO1 and pXO2, and exhibit a set of known phenotypic characteristics [ 1 - 3 ]. (plos.org)
  • However, cases of anthrax-like disease caused by non B . anthracis members of the B . cereus group have also been identified, affecting both animal and human populations [ 5 - 8 ]. (plos.org)
  • The availability of such foods could increase the prominence of Bacillus cereus as a foodborne pathogen. (highveld.com)
  • A review of the association of B cereus infections with heroin use and treatment of this pathogen is provided. (hindawi.com)
  • Bacillus cereus is a foodborne pathogen that can produce toxins, causing two types of gastrointestinal illness: the emetic (vomiting) syndrome and the diarrhoeal syndrome. (bccdc.ca)
  • Bacillus cereus is a foodborne pathogen that causes emetic or diarrheal types of food poisoning. (7thspace.com)
  • The growth and survival of B. cereus, a known pathogen commonly found in cereals, during lactic acid fermentation of mageu, a sour maize beverage, was studied. (csir.co.za)
  • It was thought to differ from Bacillus cereus , an opportunistic pathogen and cause of food poisoning, by the presence of plasmids pXO1 and pXO2, which encode the lethal toxin complex and the poly-γ- d -glutamic acid capsule, respectively. (pnas.org)
  • B. cereus is ubiquitous in nature and is an opportunistic pathogen. (pnas.org)
  • Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. (scirp.org)
  • Bacillus cereus is a relevant foodborne pathogen and biofilm producer which can contaminate and persist in the processing environment of both high and low water activity foods. (springer.com)
  • An experimental system has been designed to study the interactions between a Gram-positive biological control organism, a plant host, and a pathogen that uses recombinant inbred lines of tomato, B. cereus UW85, and the plant pathogen, Pythium torulosum Coker and Paterson. (epa.gov)
  • Introduction Bacillus cereus is a pathogen that could cause foodborne diseases. (sld.cu)
  • Objectives To evaluate the presence of B. cereus in prepared food and dehydrated foods and analyze the acceptability limits for this pathogen. (sld.cu)
  • Bacillus cereus is an important food pathogen. (scialert.net)
  • In that context, B. cereus has not generally been regarded as an important pathogen and is commonly dismissed as a laboratory contaminant. (asm.org)
  • The spices especially A. melegueta have proved very efficient in the inhibition of B. cereus a pathogen obtained from vegetable salad. (ccsenet.org)
  • B. cereus bacteria are facultative anaerobes, and like other members of the genus Bacillus, can produce protective endospores. (wikipedia.org)
  • Some strains of B. cereus produce cereins, bacteriocins active against different B. cereus strains or other Gram-positive bacteria. (wikipedia.org)
  • When consuming foods contaminated by B. cereus, bacteria and/or toxins are ingested which can result in gastro-intestinal disease in humans. (bund.de)
  • The study focused on 339 B. cereus strains isolated from food samples analysed during 74 FBOs where no other pathogenic bacteria were detected. (foodnavigator.com)
  • If you ever thought you had the 24-hour flu, you may have actually had a foodborne illness caused by the bacteria Bacillus cereus . (osu.edu)
  • The antimicrobial spectrum was relatively broad because LysB4 lysed Gram-positive bacteria such as B. cereus, Bacillus subtilis and Listeria monocytogenes and some Gram-negative bacteria when treated with EDTA. (7thspace.com)
  • The properties of LysB4 showed that this endolysin has strong lytic activity against a broad range of pathogenic bacteria, which makes LysB4 a good candidate as a biocontrol agent against B. cereus and other pathogenic bacteria. (7thspace.com)
  • Editorial Note: B. cereus, a ubiquitous, spore-forming bacteria, causes two recognized forms of foodborne gastroenteritis: an emetic syndrome resembling that caused by Staphylococcus aureus and characterized by an incubation period of 1-6 hours and a diarrheal illness characterized by an incubation period of 6-24 hours (2). (cdc.gov)
  • Spores of gram positive aerobic bacteria belonging to the genus Bacillus are part of the normal flora of the dust, soil among many more environmental sources. (writingscentre.com)
  • B cereus is not only a gram positive, spore forming, aerobic bacteria but is also known to have some facultative anaerobic characteristics. (writingscentre.com)
  • In the human opportunistic bacteria Bacillus cereus, a surface protein IlsA is shown to be involved in iron acquisition from both ferritin and hemoproteins. (sigmaaldrich.com)
  • False colour scanning electron micrograph of Bacillus cereus, a species of Gram- positive, aerobic, spore-forming bacteria. (sciencephoto.com)
  • Both compounds display pH-dependent activity against selected Gram-positive bacteria, including Staphylococcus aureus, Listeria innocua, Listeria monocytogenes, Bacillus subtilis, and Bacillus cereus ATCC 10987. (wur.nl)
  • Light micrograph of a colony of Bacillus cereus, a species of Gram-positive, aerobic, spore-forming bacteria. (sciencephoto.com)
  • B. cereus and C. perfringens are both bacteria that grow rapidly at room temperature. (marlerblog.com)
  • In this study we have established a method for screening a collection of strains, from the Bacillus cereus group of bacteria, for biofilm formation, including soil isolates, strains from culture collections, reference strains, dairy isolates, and clinical strains from different types of human infections. (uio.no)
  • Little is known about genetic exchanges in natural populations of bacteria of the spore-forming Bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. (asm.org)
  • Such studies were initially undertaken with pathogenic bacteria, such as Escherichia coli (see references 23 and 29 ), Neisseria meningitidis ( 3 ), Bordetella bronchiseptica ( 19 ), and Haemophilus influenzae ( 20 ), but have since been extended to a small number of free-living bacteria, such as Bacillus subtilis ( 8 , 15 ) and Rhizobium leguminosarum ( 33 ). (asm.org)
  • The majority of food poisoning by B. cereus could be divided into two types of symptoms - the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria. (norgenbiotek.com)
  • Results indicated that the cheese which coated with polyethylene films that treated with nisin regardless whether it was inculcated or not were free of Bacillus cereus bacteria. (scialert.net)
  • The antibacterial activity of the methanol extract of Wedelia chinensis leave was studied and tested against three pathogenic Gram positive bacteria (Bacillus cereus, B. subtilis and Stapylococcus aureus) and three pathogenic Gram negative bacteria (Escherichia coli, Proteus rettgeri and Pseudomonas aeruginosa) by the disk diffusion assay and broth dilution methods. (ijpsonline.com)
  • Bacillus thuringiensis strains display a similar repertoire of the potential virulence genes on the chromosome as B. cereus sensu stricto strains and it has been shown that these genes can also be actively expressed in B. thuringiensis strains. (europa.eu)
  • Bacillus cereus and B. thuringiensis strains are usually not discriminated in clinical diagnostics or food microbiology. (europa.eu)
  • The levels of B. cereus that can be considered as a risk for consumers are also valid for B. thuringiensis . (europa.eu)
  • The Panel has recommended the application of whole genome sequencing to provide unambiguous identification of strains used as biopesticides and the detailed characterisation of outbreak strains allowing discrimination of B. thuringiensis from B. cereus . (europa.eu)
  • Data gaps include: dose-response and behavioural characteristics of B. cereus group strains and specifically of B. thuringiensis . (europa.eu)
  • The genetic diversity and relationships among 154 Bacillus cereus/B. thuringiensis isolates recovered from soil samples from five geographic areas in Norway were investigated with multilocus enzyme electrophoresis (MEE). (nih.gov)
  • We conclude that B. cereus/B. thuringiensis from soil exhibit a high degree of recombination. (nih.gov)
  • Do Multi-year Applications of Bacillus thuringiensis subsp. (springer.com)
  • Bacillus thuringiensis subsp. (springer.com)
  • Lacey LA (2007) Bacillus thuringiensis serovariety israelensis and Bacillus sphaericus for mosquito control J. Am. Mosq. (springer.com)
  • Guidi V, Patocchi N, Lüthy P, Tonolla M (2011) Distribution of Bacillus thuringiensis subsp. (springer.com)
  • Cloning of novel enterotoxin genes from Bacillus cereus and Bacillus thuringiensis. (asm.org)
  • We isolated strains of Bacillus thuringiensis and B. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. (asm.org)
  • A total of 100 B. cereus and 98 B. thuringiensis strains were isolated and characterized by electrophoresis to determine allelic composition at nine enzymatic loci. (asm.org)
  • We observed genetic differentiation between populations of B. cereus and B. thuringiensis . (asm.org)
  • Populations of a given Bacillus species- B. thuringiensis or B. cereus -were genetically more similar to each other than to populations of the other Bacillus species. (asm.org)
  • Our results suggest that the rate of gene flow was higher between strains of the same species, but that exchanges between B. cereus and B. thuringiensis were nonetheless possible. (asm.org)
  • Overall, our data indicate that it is not important for risk assessment purposes to determine whether B. cereus and B. thuringiensis belong to a single or two species. (asm.org)
  • Bacillus cereus is a Gram-positive, rod-shaped, facultatively anaerobic, motile, beta-hemolytic, spore forming bacterium commonly found in soil and food. (wikipedia.org)
  • Bacillus (B.) cereus is a spore forming bacterium which is found worldwide. (bund.de)
  • Food poisoning caused by B. cereus can be confirmed by isolation of this bacterium in food, stool or vomit samples. (bccdc.ca)
  • Bacillus cereus is a Gram-positive aerobic or facultatively anaerobic, motile, spore-forming, rod-shaped bacterium that is widely distributed environmentally. (asm.org)
  • The Gram-positive, facultative anaerobe and rod-shaped bacterium Bacillus cereus has become increasingly important as a cause of food poisoning outbreaks. (mdpi.com)
  • The objective of this research project is to better understand the relationship between the bacterium Bacillus (B.) cereus UW85 and the plants it associates with through identification of bacterial genes that are regulated in the presence of plant compounds. (epa.gov)
  • israelensis (Bti) is a soil-borne bacterium affiliated to the Bacillus cereus group (Bcg) and has been used in biocontrol products against nematoceran larvae for several decades. (springer.com)
  • Bacillus cereus is a rod-shaped, gram-positive bacterium. (norgenbiotek.com)
  • Bacillus cereus is a bacterium found within a large range of food products and usually forms through poor storage practices associated with complex, usually global, supply chains. (fapas.com)
  • Bacillus cereus, a ubiquitous bacterium, can be isolated in various starchy food items, causing both emetic and diarrhoeal disease. (scielosp.org)
  • Demezas DH, Bell J. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. (atcc.org)
  • The current taxonomy of the B. cereus group and the status of separate species mainly rely on phenotypic characteristics. (europa.eu)
  • Antiserum is unabsorbed and may cross-react with other Bacillus species. (abcam.com)
  • In 66 of the FBOs, B. cereus was isolated with other bacterial species (including S. aureus and C. perfringens) making it impossible to affirm if it was the cause. (foodnavigator.com)
  • Drobniewski, F.A. (1998) Bacillus cereus and related species. (scirp.org)
  • Many of these Bacillus species are irrelevant contaminants. (writingscentre.com)
  • nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning. (eurosurveillance.org)
  • B. cereus -positive dehydrated food including milk, wheat and special food for malnourished pregnant women as well as species with over 10 3 /g B. cereus isolates were restricted from marketing and consumption. (sld.cu)
  • Since organisms in the Bacillus cereus group can trigger different levels of concern for manufacturers, the classification and taxonomic separation of the BCG species is critical for investigations and environmental monitoring programs. (criver.com)
  • In this article, we present antibiotypes determined by the disk diffusion method and the E test, biotypes created by the use of the Vitek Bacillus Biochemical Card, and random amplified polymorphic DNA (RAPD) patterns generated by arbitrarily primed PCR (APPCR) of 59 isolates of Bacillus species, with which we successfully investigated a nosocomial pseudoepidemic caused by B. cereus . (asm.org)
  • To explore this hypothesis, we used an in silico approach to evaluate the relationship of pathogenic potential and the divergence of the SigB-dependent general stress response within the B. cereus sensu lato group, since SigB has been demonstrated to support pathogenesis in Bacillus , Listeria and Staphylococcus species. (biomedcentral.com)
  • U02893 Bacillus cereus ATCC 11778 16S rRNA gene, partial sequence. (atcc.org)
  • AF238886 Bacillus cereus strain ATCC 11778 putative VrrB (vrrB) gene, partial cds. (atcc.org)
  • Purified spores of Bacillus cereus (ATCC 11778) and Bacillus subtilis (ATCC 9372). (abcam.com)
  • Notably, B. cereus strain ATCC 14579 and the emetic B. cereus strains F4810/72 and A529 showed reduced sensitivity to both compounds, with the latter two strains displaying full resistance to cereulide. (wur.nl)
  • Reacts with spores and vegetative cells of Bacillus cereus and Bacillus subtilis. (abcam.com)
  • On the other hand, the Si contents of Bacillus coagulans and Bacillus subtilis spores were reported to be almost absent or under the detection limit ( 4 , 24 ). (asm.org)
  • The aim was to improve monitoring and investigation of B. cereus-induced FBOs, assess the risk of emerging clusters of strains and identify strain variability. (foodnavigator.com)
  • For 42 of the 74 FBOs, a unique strain pattern was identified, providing a strain collection for further analysis of the correlation between B. cereus genotypic features and associated diseases. (foodnavigator.com)
  • A/J mice challenged with B. cereus G9241 confirmed the virulence of this strain. (pnas.org)
  • In the present study, a new strain, Bacillus cereus ZY12, producing phospholipase D (PLD) was identified. (springer.com)
  • To better address the toxic properties of the Hbl complex, a system for overexpression and purification of functional, cytotoxic, recombinant (r)Hbl components L 2 , L 1 and B from E. coli was established and an nheABC deletion mutant was constructed from B. cereus reference strain F837/76. (mdpi.com)
  • Conclusions An antifungal surfactin-like lipopeptide produced by Bacillus cereus strain AK1 was purified and chemically characterized. (amrita.edu)
  • A strain of Bacillus cereus was isolated from a detergent-polluted pond. (ovid.com)
  • Plasmids were introduced into B. cereus ATCC14579 strain using the previously described electroporation protocol (Turgeon et al. (harvard.edu)
  • Bacillus cereus strain BP01 is a microbial plant regulator that is used on cotton to produce higher quality cotton at lower cost. (webref.org)
  • Bacillus cereus is a ubiquitous organism that often contaminates microbiological cultures but rarely causes serious infections. (nih.gov)
  • Moreover, B. cereus is capable of forming biofilms, which also enables the organism to persist in food producing environments. (bund.de)
  • Bacillus cereus is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections. (hindawi.com)
  • Boiling rice does not destroy B. cereus, and if the rice is allowed to remain warm before being fried (at 30-37 degrees C.), the organism multiplies & secretes exotoxins that are not destroyed in the final frying. (sciencephoto.com)
  • However, due to the wide distribution of Bacillus spores in nature (in soil, dust, water, and other animal sources) and in the hospital environment, this organism is usually considered a saprophyte or contaminant when detected in clinical specimens of different sources ( 5 , 18 ). (asm.org)
  • Analysis of our cases suggest that ocular infection with B. cereus has certain features that may allow a provisional diagnosis to be made before isolation of the organism. (bmj.com)
  • Previous outbreaks of B cereus emetic food poisoning have been associated with consumption of contaminated fried rice and may occur after ingestion of other foods. (nih.gov)
  • Three types of Bacillus cereus enterotoxins involved in foodborne outbreaks have been identified. (highveld.com)
  • Most cases of food-borne outbreaks caused by the B. cereus group have been associated with concentrations above 10 5 CFU/g. (europa.eu)
  • Seventy four Bacillus cereus outbreaks which resulted in 911 human cases over seven years in France underlines the need to improve monitoring, according to a study. (foodnavigator.com)
  • B. cereus from a national collection of 564 strains associated with 140 strong-evidence foodborne outbreaks (FBOs) in France during 2007 to 2014 were characterised. (foodnavigator.com)
  • B. cereus is the second most frequently found causative agent of confirmed and suspected foodborne outbreaks in France after Staphylococcus aureus. (foodnavigator.com)
  • Many foods have been implicated in outbreaks of foodborne illness caused by Bacillus cereus . (osu.edu)
  • Three types of B. cereus enterotoxins involved in outbreaks of foodborne illness have been identified. (asmscience.org)
  • Bacillus cereus, an infectious cause of foodborne illness, accounted for 2% of outbreaks with confirmed etiology that were reported to CDC during 1973-1987 (1). (cdc.gov)
  • Underreporting of such outbreaks is likely because illness associated with B. cereus is usually self-limiting and not severe. (cdc.gov)
  • In addition, findings of a recent survey about culture practices for outbreaks of apparent foodborne illness indicate that 20% of state public health laboratories do not make B. cereus testing routinely available (South Carolina Department of Health and Environmental Control and CDC, unpublished data, 1991). (cdc.gov)
  • The aim of this study was to identify and characterise Bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (FBOs) occurring in France during 2007 to 2014. (eurosurveillance.org)
  • Bacillus cereus phage typing as an epidemiological tool in outbreaks of food poisoning. (asm.org)
  • The real burden of B. cereus outbreaks is actually poorly known in Italy. (scielosp.org)
  • The real burden of B. cereus outbreaks is poorly known, especially because isolated strains are not always catalogued [6]. (scielosp.org)
  • In Italy, in 2005, only 3 of 230 outbreaks reported to the computerised surveillance network for communicable diseases were associated to B. cereus [7]. (scielosp.org)
  • Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. (wur.nl)
  • As B. cereus is ubiquitous in the environment, control measures should be focused on preventing growth of B. cereus and the formation of emetic toxin in the food. (bccdc.ca)
  • Bacillus cereus is an aerobic Gram-positive spore forming rod that is ubiquitous in the environment. (omicsonline.org)
  • The Bacillus cereus sensu lato group contains ubiquitous facultative anaerobic soil-borne Gram-positive spore-forming bacilli. (biomedcentral.com)
  • Cereulide and isocereulides A-G are biosynthesized as emetic toxins by Bacillus cereus via a non-ribosomal peptide synthetase (NRPS) called Ces. (nature.com)
  • Food poisoning caused by B. cereus is an acute intoxication that occurs when this microorganism produces toxins, causing two types of gastrointestinal illness: an emetic (vomiting) syndrome or a diarrhoeal syndrome. (bccdc.ca)
  • Although the presence of vegetative forms of B. cereus in food is always necessary for foodborne disease to occur, not all the B. cereus strains can produce the toxins that cause the emetic or diarrhoeal syndromes. (bccdc.ca)
  • Huang Y, Flint SH, Palmer JS (2020) Bacillus cereus spores and toxins -The potential role of biofilms. (springer.com)
  • Antimicrobials noted to be effective in the empirical management of a B. cereus infection while awaiting antimicrobial susceptibility results for the isolate include ciprofloxacin and vancomycin. (asm.org)
  • In addition B cereus causes ocular infections, skin and wound infection and a number of ocular, central line and invasive diseases such as endocarditis, pneumonia, osteomyelitis, keratitis, panophthalmitis, bacteremia, endophthalmitis, meningitis and brain abscess (Ryan et al 2004). (writingscentre.com)
  • Bacillus cereus is a gram positive microorganism commonly involved in gastrointestinal infection but capable of causing severe infections and bacteremia. (elsevier.es)
  • Find out how Bacillus cereus can make you ill and how to avoid infection. (mpi.govt.nz)
  • Bacillus cereus infection can cause nausea, vomiting and diarrhoea. (mpi.govt.nz)
  • Fulminating panophthalmitis due to exogenous infection with Bacillus cereus: report of 4 cases. (bmj.com)
  • A case of severe infection due to Bacillus cereus isolated on the tip of central venous catheter (CVC) of a patient with acute lymphoblastic leukemia (ALL) is reported here. (omicsonline.org)
  • Bacillus cereus is a rare cause of serious human infection but, paradoxically, causes one of the most severe posttraumatic or endogenous infections of the eye, endophthalmitis, which frequently results in blindness. (asm.org)
  • We therefore sought to examine the contribution of the dermonecrotic toxin, hemolysin BL, to the pathogenesis of B. cereus infection in an endophthalmitis system that is highly amenable to study. (asm.org)
  • The pathogenesis of infection resulting from intravitreal injection of 10 2 CFU of either a clinical ocular isolate of B. cereus producing hemolysin BL (HBL + ) or an isogenic mutant in this trait (HBL − ) was assessed bacteriologically and by slit lamp biomicroscopy, electroretinography, histology, and inflammatory cell enumeration. (asm.org)
  • B. cereus ranks as a leading cause of posttraumatic endophthalmitis, a potentially blinding infection of the tissues of the interior of the eye, resulting from intraocular contamination during surgery or penetrating injury ( 1 , 7 , 30 , 36 , 41 ). (asm.org)
  • While B. cereus is associated mainly with food poisoning, it is being increasingly reported to be a cause of serious and potentially fatal non-gastrointestinal-tract infections. (asm.org)
  • Bacillus cereus wound infections. (bmj.com)
  • Données relatives aux toxi-infections alimentaires collectives déclarées en France en 2013. (eurosurveillance.org)
  • In the past several years, however, nongastrointestinal B. cereus infections have been reported with increasing frequency, perhaps because of an increasing awareness of the pathogenic potential of this saprophyte. (asm.org)
  • The genus Bacillus, Agriculture Handbook 427. (atcc.org)
  • The pathogenic spectrum of B. cereus ranges from strains used as probiotics to those lethal to humans and it remains difficult to predict pathogenic potential, said the researchers. (foodnavigator.com)
  • However, the pathogenic potential of the B cereus stands out quite greatly. (writingscentre.com)
  • The possibility to discriminate between pathogenic and non-pathogenic B. cereus strains could lead to a re-evaluation of tolerance levels for B. cereus in food commodities. (openrepository.com)
  • However, the pathogenic importance of this three-component enterotoxin is difficult to access, as nearly all natural B. cereus culture supernatants additionally contain the highly cytotoxic Nhe, the second three-component toxin involved in the aetiology of B. cereus -induced food-borne diseases. (mdpi.com)
  • The emetic syndrome will affect consumers of food contaminated with the emetic toxin cereulide, therefore the food needs to be contaminated with B. cereus strains that are able produce this toxin and be handled in a way that allows bacterial growth and subsequent toxin formation. (bccdc.ca)
  • Data from analytical ultracentrifuge studies suggest that polymers of ribosomes exist in dormant spores of Bacillus cereus T. (sciencemag.org)
  • citation needed] The timing of the toxin production was previously thought to be possibly responsible for the two different courses of disease, but in fact the emetic syndrome is caused by a toxin, cereulide, found only in emetic strains and is not part of the "standard toolbox" of B. cereus. (wikipedia.org)
  • Only a minority of B. cereus strains is capable of producing cereulide. (bund.de)
  • UPLC-TOF MS analysis of B. cereus strains in combination with 13 C-labeling experiments now revealed tetra-, octa- and dodecapeptides of a different sequence, namely (L- O -Val-L-Val-D- O -Leu-D-Ala) 1-3 , as intermediates of cereulide biosynthesis. (nature.com)
  • It is estimated that, in order to produce sufficient cereulide to induce vomiting, levels of B. cereus should be greater than 10,000 per gram of food, but several publications have documented illnesses, including hospitalizations with lower numbers. (bccdc.ca)
  • title=The cereulide genetic determinants of emetic ''Bacillus cereus'' are plasmid-borne. (wikidoc.org)
  • Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus. (eurosurveillance.org)
  • The putative role of cereulide production in the ecology of emetic B. cereus is discussed. (wur.nl)
  • A single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by Bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. (mdpi.com)
  • Inoculation of samples with three cereulide toxin-producing strains of Bacillus cereus was finally used to mimic naturally contaminated foods. (mdpi.com)
  • To develop a rapid and sensitive detection method for cereulide-producing Bacillus cereus using a real-time PCR based on the sequence of the cereulide synthesis gene. (ovid.com)
  • A total of 56 cereulide-producing B. cereus and 15 cereulide-negative strains were tested. (ovid.com)
  • We designed specific primers and probes for the detection of cereulide-producing B. cereus. (ovid.com)
  • The new cycleave real-time PCR assay gave positive detections for all of 56 cereulide-producing B. cereus strains, whereas all other strains including 10 systemic infectious disease strains were negative. (ovid.com)
  • The performance of the assay was highly reproducible and specific for cereulide-producing B. cereus. (ovid.com)
  • B. cereus strains isolated from foodstuff suspected of being involved in an FBO are usually collected by the laboratory for food safety in ANSES (Agency for Food, Environmental and Occupational Health and Safety). (foodnavigator.com)
  • Hussain MS, Oh DH (2017) Substratum attachment location and biofilm formation by Bacillus cereus strains isolated from different sources: effect on total biomass production and sporulation in different growth conditions. (springer.com)
  • In this study, in silico analysis of B. cereus FlhF revealed that this protein presents conserved domains that are typical of SRPs in many organisms and a peculiar N-terminal basic domain. (frontiersin.org)
  • Our work highlights the multifunctional role of FlhF in B. cereus , being this protein involved in bacterial flagellation, swarming, protein secretion, and pathogenicity. (frontiersin.org)
  • SOXA Recombinant Protein (Bacillus sp. (avivasysbio.com)
  • An important role for the complete Isd system in heme-associated bacterial growth is demonstrated and new insights into the interplay between an Isd NEAT surface protein and an IlsA-NEAT-LRR protein, both of which appear to be involved in heme-iron acquisition in B. cereus are revealed. (sigmaaldrich.com)
  • We compared the protein-coding content of 201 B. cereus sensu lato genomes to characterize differences and understand the consequences of these differences on biological function. (biomedcentral.com)
  • Also known as the 'long-incubation' form of B. cereus food poisoning, it might be difficult to differentiate from poisoning caused by Clostridium perfringens. (wikipedia.org)
  • The symptoms of bacillus diarrheal type mimic those of clostridium perfringens. (calrest.org)
  • Some Bacillus cereus or Clostridium perfringens with your Rancho Bravo Taco? (marlerblog.com)
  • Symptoms and timing of illness onset are suggestive of a bacterial toxin from Bacillus cereus or Clostridium perfringens. (marlerblog.com)
  • Kwon M, Hussain MS, Oh DH (2017) Biofilm formation of Bacillus cereus under food-processing-related conditions. (springer.com)
  • Periodontal isolates of ''B. cereus'' also possess distinct pXO1-like plasmids. (wikidoc.org)
  • Ceuppens S, Boon N, Uyttendaele M (2013) Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles FEMS Microbiol. (springer.com)
  • Rasko D, Altherr M, Han C, Ravel J (2005) Genomics of the Bacillus cereus group of organisms FEMS Microbiol. (springer.com)
  • In food animals such as chickens, rabbits and pigs, some harmless strains of B. cereus are used as a probiotic feed additive to reduce Salmonella in the animals' intestines and cecum. (wikipedia.org)
  • At 30 °C (86 °F), a population of B. cereus can double in as little as 20 minutes or as long as 3 hours, depending on the food product. (wikipedia.org)
  • Bacillus foodborne illnesses occur due to survival of the bacterial endospores when infected food is not, or inadequately, cooked. (wikipedia.org)
  • Emetic food poisoning caused by Bacillus cereus. (nih.gov)
  • Epidemiologic investigation incriminated macaroni and cheese as a cause of the illness and samples of this food contained large numbers of Bacillus cereus. (nih.gov)
  • Diarrheal disease: Here, high numbers of cells and/or spores of B. cereus are ingested with the food and, after germination of spores in the small intestine, the vegetative cells begin producing enterotoxins. (bund.de)
  • However, consumer interest in precooked chilled food products with a long shelf life may lead to products well suited for Bacillus cereus survival and growth. (highveld.com)
  • Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. (atcc.org)
  • Unilever is working with both the Canadian Food Inspection Agency (CFIA) and the U.S. Food and Drug Administration (FDA) on the Slim Fast recall because of possible Bacillus cereus contamination. (foodsafetynews.com)
  • Food contaminated with Bacillus cereus may not look or smell spoiled. (foodsafetynews.com)
  • The symptoms of Bacillus cereus food poisoning can include vomiting or diarrhea, abdominal cramps and pain and may occur between 30 minutes and 15 hours after consuming the contaminated product. (foodsafetynews.com)
  • We recommend collecting at least five colonies from each food sample potentially contaminated with B. cereus, with different morphologies, as several B. cereus with different genetic characteristics may be present in the same food product. (foodnavigator.com)
  • B. cereus will grow in food that has been improperly stored, therefore proper food handling, especially after cooking, will help prevent illnesses caused by this microorganism. (bccdc.ca)
  • The diarrhoeal syndrome occurs when enterotoxins are produced in the intestine, following ingestion of food contaminated with B. cereus . (bccdc.ca)
  • The diarrhoeal syndrome occurs when a large number of vegetative cells of B. cereus (at least 10,000 per gram of food) are ingested and produce enterotoxin in the small intestine. (bccdc.ca)
  • The CDC has calculated an estimate of the number of cases of Bacillus cereus based on corrections for underreporting, misdiagnosis, and the number of cases that are not caused by contaminated food. (osu.edu)
  • The CDC estimates that there are over 63,000 cases of Bacillus cereus illness each year in this country, and that 100% of the cases are caused by eating contaminated food. (osu.edu)
  • Anyone can get a foodborne illness from eating a food contaminated with Bacillus cereus . (osu.edu)
  • Bacillus cereus food poisoning is the general description, although two recognized types of illness are caused by two distinct metabolites. (calrest.org)
  • The incidence of B. cereus food poisoning has been gradually increasing over the past few years, therefore, biocontrol agents effective against B. cereus need to be developed. (7thspace.com)
  • To prevent Bacillus cereus food poisoning, store, handle and cool food safely . (foodsafety.asn.au)
  • Generally speaking, ''Bacillus'' foodborne illnesses occur due to survival of the bacterial spores when food is improperly cooked. (wikidoc.org)
  • The diagnosis of B. cereus food poisoning can be confirmed by the isolation of greater than or equal to 105 B. cereus organisms per gram from epidemiologically implicated food. (cdc.gov)
  • Fried rice is a leading cause of B. cereus emetic-type food poisoning in the United States (1,4). (cdc.gov)
  • Persons suffering from B cereus food poisoning only require supportive treatment. (writingscentre.com)
  • Bacillus cereus is a ubiquitary microorganism that may cause food borne disease. (openrepository.com)
  • Fatal family outbreak of Bacillus cereus-associated food poisoning. (eurosurveillance.org)
  • B. cereus is an important cause of food poisoning, occurring in soil & therefore on vegetables eaten by humans, notably rice. (sciencephoto.com)
  • Forty seven samples of dehydrated foodstuffs showed B. cereus at a rate of 10 2 /g in most of them, thus indicating good food quality. (sld.cu)
  • Conclusions Prepared food dishes in which B. cereus was isolated did not meet the acceptability criteria. (sld.cu)
  • The Bacillus cereus group (BCG) is of great concern to pharma, food, and consumer care industries because members are often identified as contaminants in many manufacturing facilities and food products. (criver.com)
  • Contagion and contagiousness refers to how easily the spread of Bacillus cereus type I food poisoning is possible from one person to another. (rightdiagnosis.com)
  • B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. (norgenbiotek.com)
  • The increase in B. cereus -induced FBOs is partly due to the input of national health and food safety authorities in the epidemiological and microbiological investigations of suspected FBOs. (pubmedcentralcanada.ca)
  • We recommend this assay for routine examination of suspected B. cereus food poisonings. (ovid.com)
  • There are two principal types of food poisoning caused by B. cereus , namely diarrheal and emetic types of food poisoning. (scialert.net)
  • Under the Microbiological Guidelines for Food, if ready-to-eat food contains Bacillus cereus at a level of more than 100 000 per gram, it is considered unsatisfactory. (gov.hk)
  • Consuming food contaminated with excessive Bacillus cereus may cause gastrointestinal upset such as vomiting and diarrhoea. (gov.hk)
  • Bacillus cereus is associated primarily with cases of food-borne gastrointestinal illnesses that are usually self-limiting and are rarely life-threatening. (asm.org)
  • Bacillus cereus is ubiquitously distributed in the envi-ronment [1] and can be isolated in various starchy food items ( e.g ., milk, rice, and pasta). (scielosp.org)
  • B. cereus was isolated in specimens of ricotta cheese collected at the dairy food supplier, but no laboratory confirmation was possible as no food leftovers were available. (scielosp.org)
  • Besides sporulation, Bacillus cereus can undergo a differentiation process in which short swimmer cells become elongated and hyperflagellated swarmer cells that favor migration of the bacterial community on a surface. (frontiersin.org)
  • Turgeon N, Laflamme C, Ho J, Duchaine C. Evaluation of the plasmid copy number in B. cereus spores, during germination, bacterial growth and sporulation using real-time PCR. (harvard.edu)
  • Bacillus cereus is a Gram-positive, facultatively aerobic sporeformer whose cells are large rods and whose spores do not swell the sporangium. (abcam.com)
  • This quantitative proficiency test evaluates your testing ability regarding an Aerobic Plate Count and the enumeration of Bacillus cereus in beef. (fapas.com)
  • As a result of contamination with spore containing soil particles or dust, B. cereus can be easily transmitted to foods. (bund.de)
  • Bacillus cereus is a normal soil inhabitant and is frequently isolated from a variety of foods, including vegetables, dairy products and meat. (highveld.com)
  • B. cereus is widespread in the environment and commonly found in the soil. (bccdc.ca)
  • Bacillus cereus form heat resistant spores that are widespread in our environment and are common in soil and dust. (foodsafety.asn.au)
  • Bacillus cereus is a Gram-positive, motile, spore-bearing rod, frequently isolated from the soil, where the spore ensures its persistence under adverse conditions. (frontiersin.org)
  • In the present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. (scirp.org)
  • Biosynthesis and characterization of polyhydroxyalkanoate from marine Bacillus cereus MCCB 281 utilizing glycerol as carbon source. (sigmaaldrich.com)
  • The aim of this study was to evaluate the efficacy of dry sanitizing treatments against B. cereus biofilm formed on stainless steel (SS) and polypropylene (PP). Biofilm formation was held through the static method at 25 °C. After 4 days of incubation, coupons were exposed for up to 30 min to UV-C light, dry heat, gaseous ozone, 70% ethanol, and a commercial sanitizer. (springer.com)
  • Hussain MS, Oh DH (2018) Impact of the isolation source on the biofilm formation characteristics of Bacillus cereus . (springer.com)
  • Pagedar A, Singh J (2012) Influence of physiological cell stages on biofilm formation by Bacillus cereus of dairy origin. (springer.com)
  • Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. (norgenbiotek.com)
  • In addition to these 44 isolates of B. cereus , 12 epidemiologically unrelated B. cereus isolates, one Bacillus sphaericus isolate from a blood specimen from a patient seen in May 1990, and two B. sphaericus isolates from 95% alcohol in the liquor factory were also studied for their microbiological relatedness. (asm.org)
  • B. cereus is responsible for a minority of foodborne illnesses (2-5%), causing severe nausea, vomiting, and diarrhea. (wikipedia.org)
  • Most illnesses from Bacillus cereus are caused by eating contaminated rice or starchy foods such as potato flakes and pasta. (mpi.govt.nz)
  • During the divergence of these organisms from a common "SigB-less" ancestor, the placement of SigB promoters at varied locations in the B. cereus sensu lato genomes predict alternative structures for the SigB regulon in different organisms. (biomedcentral.com)
  • 2005. Opinion of the Scientific Panel on biological hazards (BIOHAZ) on Bacillus cereus and other Bacillus spp in foodstuffs . (cambridge.org)
  • Heme interplay between IlsA and IsdC: Two structurally different surface proteins from Bacillus cereus. (sigmaaldrich.com)
  • Isothermal titration calorimetry, UV-Vis spectrophotometry, affinity chromatography and rapid kinetics stopped-flow measurements were employed to probe the binding and transfer of hemin between two different B. cereus surface proteins (IlsA and IsdC). (sigmaaldrich.com)
  • For the first time, it is shown that two different B. cereus surface proteins (IlsA and IsdC) can interact and transfer heme suggesting their involvement in B. cereus heme acquisition. (sigmaaldrich.com)
  • The messenger activity of total RNA from normal and 8-azaguanine-treated Bacillus cereus cultures was measured by the stimulation of amino acid incorporation into proteins in the preincubated S-30 Escherichia coli system. (aspetjournals.org)
  • Previous studies have demonstrated the efficacy of our Bacillus cereus isolate (NRRL 100132) in reducing concentrations of nitrogenous wastes and inhibiting growth of fish pathogens. (csir.co.za)
  • Through reliable detection of a broad range of B . cereus group isolates causing anthrax-like disease it will be possible to assess the distribution and diversity of these pathogens and their impact on public health and wildlife populations. (plos.org)
  • B. cereus was isolated from leftover chicken fried rice (greater than 10 superscript 6 organisms per gram) and from vomitus from one ill child ( greater than 10 superscript 5 organisms per gram) but not from samples of leftover milk. (cdc.gov)
  • The test result showed that the sample contained Bacillus cereus at a level of 3.8 million per gram. (gov.hk)
  • Many questions about the epidemiology, biology and impact of this cluster of anthrax causing B . cereus still remain open. (plos.org)
  • Sushi rice usually contains sushi vinegar and a mixture of sugar and salt, which helps to prevent the growth of the Bacillus cereus . (mpi.govt.nz)
  • Growth inhibition of B. cereus . (asm.org)
  • These and other characteristics, including biochemical features, are used to differentiate and confirm the presence B. cereus, although these characteristics are shared with B. cereus var. (abcam.com)
  • The crystals of beta-amylase from Bacillus cereus belong to space group P21 with the following cell dimensions: a = 57.70 A, b = 92.87 A, c = 65.93 A, and beta =101.95 degrees. (rcsb.org)
  • Some strains of the Bacillus cereus group are able to grow at refrigeration temperatures. (highveld.com)
  • The Bacillus cereus sensu lato group is genetically very close and possesses a remarkable plasmid gene pool that encodes a variety of functions such as virulence and self-transfer capabilities. (nih.gov)
  • israelensis for Control of Mosquito Larvae Affect the Abundance of B. cereus Group Populations in Riparian Wetland Soils? (springer.com)
  • What can you do to ensure you're correctly identifying members of the B. cereus group? (criver.com)
  • This is consistent with the hypothesis that divergence within the B. cereus sensu lato group rests in part on alternative strategies for regulation of a common gene pool. (biomedcentral.com)
  • Our study provides a convenient molecular scheme for characterisation of B. cereus strains responsible for FBOs in order to improve the monitoring and investigation of B. cereus -induced FBOs, assess emerging clusters and diversity of strains. (eurosurveillance.org)
  • To the best of our knowledge, only one previous report has described the application of molecular analysis, i.e., pulsed-field gel electrophoresis (PFGE), to successfully identify a pseudo-outbreak due to B. cereus and to trace the source of contamination ( 11 ). (asm.org)
  • The antimicrobial effects of the supernatants obtained from four strains of bifidobacteria were tested against two types of indicator strains, Bacillus cereus and three strains of Escherichia coli . (scialert.net)
  • Most of starchy foods are closely linked with emetic B cereus toxin outbreak. (writingscentre.com)
  • We report a B. cereus foodborne outbreak that occurred in a pub in Bari (Italy) on January 22nd 2012 during a birthday party, promptly reported by the pub owner. (scielosp.org)
  • We report a B. cereus foodborne outbreak that occurred during a birthday party in a pub in Bari (Puglia, South Italy). (scielosp.org)