Substances that are recognized by the immune system and induce an immune reaction.
Substances elaborated by bacteria that have antigenic activity.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances elaborated by viruses that have antigenic activity.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.
Substances of fungal origin that have antigenic activity.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.
The major group of transplantation antigens in the mouse.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
Those proteins recognized by antibodies from serum of animals bearing tumors induced by viruses; these proteins are presumably coded for by the nucleic acids of the same viruses that caused the neoplastic transformation.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.
Antibodies produced by a single clone of cells.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Large, transmembrane, non-covalently linked glycoproteins (alpha and beta). Both chains can be polymorphic although there is more structural variation in the beta chains. The class II antigens in humans are called HLA-D ANTIGENS and are coded by a gene on chromosome 6. In mice, two genes named IA and IE on chromosome 17 code for the H-2 antigens. The antigens are found on B-lymphocytes, macrophages, epidermal cells, and sperm and are thought to mediate the competence of and cellular cooperation in the immune response. The term IA antigens used to refer only to the proteins encoded by the IA genes in the mouse, but is now used as a generic term for any class II histocompatibility antigen.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Sites on an antigen that interact with specific antibodies.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*02 allele family.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Molecules on the surface of B- and T-lymphocytes that recognize and combine with specific antigens.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Glycoproteins expressed on cortical thymocytes and on some dendritic cells and B-cells. Their structure is similar to that of MHC Class I and their function has been postulated as similar also. CD1 antigens are highly specific markers for human LANGERHANS CELLS.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Class I human histocompatibility (HLA) surface antigens encoded by more than 30 detectable alleles on locus B of the HLA complex, the most polymorphic of all the HLA specificities. Several of these antigens (e.g., HLA-B27, -B7, -B8) are strongly associated with predisposition to rheumatoid and other autoimmune disorders. Like other class I HLA determinants, they are involved in the cellular immune reactivity of cytolytic T lymphocytes.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A melanosome-specific protein that plays a role in the expression, stability, trafficking, and processing of GP100 MELANOMA ANTIGEN, which is critical to the formation of Stage II MELANOSOMES. The protein is used as an antigen marker for MELANOMA cells.
Antigens associated with specific proteins of the human adult T-cell immunodeficiency virus (HIV); also called HTLV-III-associated and lymphadenopathy-associated virus (LAV) antigens.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CTLA-4 ANTIGEN with high specificity and to CD28 ANTIGEN with low specificity. The interaction of CD80 with CD28 ANTIGEN provides a costimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
An encapsulated lymphatic organ through which venous blood filters.
A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
A glycolipid, cross-species antigen that induces production of antisheep hemolysin. It is present on the tissue cells of many species but absent in humans. It is found in many infectious agents.
The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.
A sex-specific cell surface antigen produced by the sex-determining gene of the Y chromosome in mammals. It causes syngeneic grafts from males to females to be rejected and interacts with somatic elements of the embryologic undifferentiated gonad to produce testicular organogenesis.
The sum of the weight of all the atoms in a molecule.
A heterogeneous group of immunocompetent cells that mediate the cellular immune response by processing and presenting antigens to the T-cells. Traditional antigen-presenting cells include MACROPHAGES; DENDRITIC CELLS; LANGERHANS CELLS; and B-LYMPHOCYTES. FOLLICULAR DENDRITIC CELLS are not traditional antigen-presenting cells, but because they hold antigen on their cell surface in the form of IMMUNE COMPLEXES for B-cell recognition they are considered so by some authors.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
A costimulatory ligand expressed by ANTIGEN-PRESENTING CELLS that binds to CD28 ANTIGEN with high specificity and to CTLA-4 ANTIGEN with low specificity. The interaction of CD86 with CD28 ANTIGEN provides a stimulatory signal to T-LYMPHOCYTES, while its interaction with CTLA-4 ANTIGEN may play a role in inducing PERIPHERAL TOLERANCE.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
An inhibitory T CELL receptor that is closely related to CD28 ANTIGEN. It has specificity for CD80 ANTIGEN and CD86 ANTIGEN and acts as a negative regulator of peripheral T cell function. CTLA-4 antigen is believed to play role in inducing PERIPHERAL TOLERANCE.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A component of the B-cell antigen receptor that is involved in B-cell antigen receptor heavy chain transport to the PLASMA MEMBRANE. It is expressed almost exclusively in B-LYMPHOCYTES and serves as a useful marker for B-cell NEOPLASMS.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Sialylated Lewis blood group carbohydrate antigen found in many adenocarcinomas of the digestive tract, especially pancreatic tumors.
Proteins prepared by recombinant DNA technology.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
A melanosome-associated protein that plays a role in the maturation of the MELANOSOME.
Diagnostic procedures involving immunoglobulin reactions.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
Immunoglobulins produced in a response to HELMINTH ANTIGENS.
Antigens which may directly stimulate B lymphocytes without the cooperation of T lymphocytes.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Glycoprotein members of the immunoglobulin superfamily which participate in T-cell adhesion and activation. They are expressed on most peripheral T-lymphocytes, natural killer cells, and thymocytes, and function as co-receptors or accessory molecules in the T-cell receptor complex.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
A closely related group of antigens found in the plasma only during the infective phase of hepatitis B or in virulent chronic hepatitis B, probably indicating active virus replication; there are three subtypes which may exist in a complex with immunoglobulins G.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Costimulatory T-LYMPHOCYTE receptors that have specificity for CD80 ANTIGEN and CD86 ANTIGEN. Activation of this receptor results in increased T-cell proliferation, cytokine production and promotion of T-cell survival.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Carbohydrate antigen most commonly seen in tumors of the ovary and occasionally seen in breast, kidney, and gastrointestinal tract tumors and normal tissue. CA 125 is clearly tumor-associated but not tumor-specific.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Immunologically detectable substances found in the CELL NUCLEUS.
Allelic alloantigens often responsible for weak graft rejection in cases when (major) histocompatibility has been established by standard tests. In the mouse they are coded by more than 500 genes at up to 30 minor histocompatibility loci. The most well-known minor histocompatibility antigen in mammals is the H-Y antigen.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*27 allele family.
The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Antigens produced by various strains of HEPATITIS D VIRUS.
Class I human histocompatibility (HLA) antigens encoded by a small cluster of structural genes at the C locus on chromosome 6. They have significantly lower immunogenicity than the HLA-A and -B determinants and are therefore of minor importance in donor/recipient crossmatching. Their primary role is their high-risk association with certain disease manifestations (e.g., spondylarthritis, psoriasis, multiple myeloma).
Glycoproteins with a wide distribution on hematopoietic and non-hematopoietic cells and strongly expressed on macrophages. CD58 mediates cell adhesion by binding to CD2; (ANTIGENS, CD2); and this enhances antigen-specific T-cell activation.
A major histocompatibily complex class I-like protein that plays a unique role in the presentation of lipid ANTIGENS to NATURAL KILLER T-CELLS.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*01 allele family.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*07 allele family.
Polysaccharides found in bacteria and in capsules thereof.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*04 alleles.
An HLA-DR antigen which is associated with HLA-DRB1 CHAINS encoded by DRB1*03 alleles.
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Glycoproteins found on the membrane or surface of cells.
Glycoproteins expressed on all mature T-cells, thymocytes, and a subset of mature B-cells. Antibodies specific for CD5 can enhance T-cell receptor-mediated T-cell activation. The B-cell-specific molecule CD72 is a natural ligand for CD5. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Tumors or cancer of the PROSTATE.
Unglycosylated phosphoproteins expressed only on B-cells. They are regulators of transmembrane Ca2+ conductance and thought to play a role in B-cell activation and proliferation.
A member of the tumor necrosis factor receptor superfamily found on most T-LYMPHOCYTES. Activation of the receptor by CD70 ANTIGEN results in the increased proliferation of CD4-POSITIVE T-LYMPHOCYTES and CD8-POSITIVE T-LYMPHOCYTES. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*24 allele family.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Elements of limited time intervals, contributing to particular results or situations.
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
A specific immune response elicited by a specific dose of an immunologically active substance or cell in an organism, tissue, or cell.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Proteins found in any species of bacterium.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
T-cell receptors composed of CD3-associated gamma and delta polypeptide chains and expressed primarily in CD4-/CD8- T-cells. The receptors appear to be preferentially located in epithelial sites and probably play a role in the recognition of bacterial antigens. The T-cell receptor gamma/delta chains are separate and not related to the gamma and delta chains which are subunits of CD3 (see ANTIGENS, CD3).
Differentiation antigens expressed on pluripotential hematopoietic cells, most human thymocytes, and a major subset of peripheral blood T-lymphocytes. They have been implicated in integrin-mediated cellular adhesion and as signalling receptors on T-cells.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
A HLA-DR antigen that is associated with HLA-DRB1 CHAINS encoded by DRB1*07 alleles.
Antigens from any of the hepatitis viruses including surface, core, and other associated antigens.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Antibodies from an individual that react with ISOANTIGENS of another individual of the same species.
A specific HLA-A surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-A*03 allele family.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
An integrin alpha subunit of approximately 150-kDa molecular weight. It is expressed at high levels on monocytes and combines with CD18 ANTIGEN to form the cell surface receptor INTEGRIN ALPHAXBETA2. The subunit contains a conserved I-domain which is characteristic of several of alpha integrins.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement.
Genetic loci in the vertebrate major histocompatibility complex that encode polymorphic products which control the immune response to specific antigens. The genes are found in the HLA-D region in humans and in the I region in mice.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
Class I-restricted activation of CD8-POSITIVE LYMPHOCYTES resulting from ANTIGEN PRESENTATION of exogenous ANTIGENS (cross-presentation). This is in contrast to normal activation of these lymphocytes (direct-priming) which results from presentation of endogenous antigens.
A specific HLA-B surface antigen subtype. Members of this subtype contain alpha chains that are encoded by the HLA-B*44 allele family.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

Characterization of mouse and human B7-H3 genes. (1/88)

T cell activation and immune function are regulated by costimulatory molecules of the B7 superfamily. Human B7-H3 is a recent addition to this family and has been shown to mediate T cell proliferation and IFN-gamma production. In this work we describe the identification of the mouse B7-H3 homolog, which is ubiquitously expressed in a variety of tissues. Activated CD4 and CD8 T cells express a putative receptor that can be recognized by soluble mouse B7-H3-Ig molecules. While the mouse B7-H3 gene was found to contain a single copy, we discovered a novel isoform of human B7-H3 (named as B7-H3b hereafter) with four Ig-like domains that results from gene duplication and differential splicing. B7-H3b is the major isoform expressed in several tissues. This structural information suggests a genetic variation of the B7-H3 gene in mammalian species.  (+info)

Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains. (2/88)

In an effort to characterize molecules with immunoregulatory potential, we raised mAbs to human dendritic cells. We selected an Ab that recognizes a molecule that is induced on monocytes differentiated in vitro toward dendritic cells. Retroviral expression cloning identified this molecule as B7-H3, a member of the B7 family described recently. In contrast to an earlier report, in which B7-H3 was described as a molecule consisting of two Ig-like domains, our cDNA encoded a type I membrane protein with four Ig-like domains, and the molecule identified by us was therefore named 4Ig-B7-H3. mRNA analysis as well as Western blotting experiments performed by us did not reveal evidence for a small B7-H3. B7-H3 is not expressed on peripheral blood lymphocytes, monocytes, or granulocytes. Upon in vitro stimulation, the expression of B7-H3 is induced on T cells, B cells, and NK cells. A number of different approaches were used to investigate the function of human B7-H3. In contrast to an earlier report, our data do not support a costimulatory role of B7-H3 in anti-CD3-mediated activation of the TCR-complex resulting in T cell proliferation and IFN-gamma production.  (+info)

Murine B7-H3 is a negative regulator of T cells. (3/88)

T cell activation is regulated by the innate immune system through positive and negative costimulatory molecules. B7-H3 is a novel B7-like molecule with a putative receptor on activated T cells. Human B7-H3 was first described as a positive costimulator, most potently inducing IFN-gamma production and cellular immunity. In this study we examined the expression and function of mouse B7-H3. B7-H3 is mostly expressed on professional APCs; its expression on dendritic cells appears to be up-regulated by LPS. In contrast to human B7-H3, we found that mouse B7-H3 protein inhibited T cell activation and effector cytokine production. An antagonistic mAb to B7-H3 enhanced T cell proliferation in vitro and led to exacerbated experimental autoimmune encephalomyelitis in vivo. Therefore, mouse B7-H3 serves as a negative regulator of T cell activation and function.  (+info)

Identification of 4Ig-B7-H3 as a neuroblastoma-associated molecule that exerts a protective role from an NK cell-mediated lysis. (4/88)

In this study, in an attempt to identify neuroblastoma-associated surface antigens, we generated mAbs against the ACN neuroblastoma cell line. A mAb was selected (5B14) that reacted with all neuroblastoma cell lines analyzed and allowed detection of tumor cell infiltrates in bone marrow aspirates from neuroblastoma patients. In cytofluorimetric analysis, unlike anti-disialoganglioside mAb, 5B14 mAb did not display reactivity with normal bone marrow hematopoietic cell precursors, thus representing a highly specific marker for identifying neuroblastoma cells. Molecular analysis revealed that the 5B14 mAb-reactive surface glycoprotein corresponded to the recently identified 4Ig-B7-H3 molecule. Remarkably, mAb-mediated masking of the 4Ig-B7-H3 molecule on cell transfectants or on freshly isolated neuroblastoma cells resulted in enhancement of natural killer-mediated lysis of these target cells. These data suggest that 4Ig-B7-H3 molecules expressed at the tumor cell surface can exert a protective role from natural killer-mediated lysis by interacting with a still undefined inhibitory receptor expressed on natural killer cells.  (+info)

The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. (5/88)

B7-H3, a member of the B7 family of the Ig superfamily proteins, is expressed on the surface of the antigen-presenting cells and down-regulates T cell functions by engaging an unknown counterreceptor on T cells. Although B7-H3 is ubiquitously expressed, its potential nonimmune functions have not been addressed. We found that B7-H3 is highly expressed in developing bones during embryogenesis and that its expression increases as osteoblast precursor cells differentiate into mature osteoblasts. In vitro bone formation by osteoblastic cells was inhibited when B7-H3 function was interrupted by the soluble recombinant protein B7-H3-Fc. Analysis of calvarial cells derived from neonatal B7-H3 knockout (KO) mice revealed normal numbers of osteoblast precursor cells possessing a normal proliferative capacity. However, the B7-H3-deficient calvarial cells exhibited impaired osteogenic differentiation, resulting in decreased mineralized bone formation in vitro. These results suggest that B7-H3 is required for the later phase of osteoblast differentiation. Although B7-H3 KO mice had no gross skeletal abnormalities, they displayed a lower bone mineral density in cortical (but not trabecular) bones compared with WT controls. Consistent with the reduced bone mineral density, the femurs of B7-H3 KO mice were more susceptible to bone fracture compared with those of WT mice. Taken together, these results indicate that B7-H3 and its unknown counterreceptor play a positive regulatory role in bone formation. In addition, our findings identified B7-H3 as another molecule that has a dual role in the bone-immune interface.  (+info)

B7-H3 enhances tumor immunity in vivo by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells. (6/88)

B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.  (+info)

Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells. (7/88)

Activated T cells have been implicated in chronic rhinosinusitis (CRS) and asthma and physically interact with epithelial cells in the airways. We now report that human airway epithelial cells display significant constitutive cell-surface expression of costimulatory ligands, B7-H1, B7-H2, B7-H3, and B7-DC. Expression of B7-H1 and B7-DC was selectively induced by stimulation of either BEAS2B or primary nasal epithelial cells (PNEC) with interferon (IFN)-gamma (100 ng/ml). The combination of IFN-gamma and tumor necrosis factor-alpha (100 ng/ml) selectively induced expression better than IFN-gamma alone. Fluticasone treatment (10(-7) M) reduced the baseline expression and inhibited the induction of B7-H1 and B7-DC in BEAS2B cells. In vitro exposure of PNEC to IFN-gamma also resulted in selective induction of B7-H1 and B7-DC. Monoclonal antibody blockade of B7-H1 or B7-DC enhanced IFN-gamma expression by purified T cells in co-culture experiments, suggesting that these two B7 homologs inhibit T cell responses at the mucosal surface. Immunohistochemical staining of human sinonasal surgical tissue confirmed the presence of B7-H1, B7-H2, and B7-H3 in the epithelial cell layer, especially in samples from patients diagnosed with Samter's Triad, a severe form of CRS. Real-time PCR analysis of sinonasal tissue revealed elevated levels of B7-H1 and B7-DC in CRS compared with controls. These results demonstrate that epithelial cells express functional B7 costimulatory molecules and that expression of selected B7 family members is inducible in vitro and in vivo. Epithelial B7 homologs could play a role in regulation of lymphocytic activity at mucosal surfaces.  (+info)

The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta. (8/88)

Placental trophoblast cells form a cellular barrier between the potentially immunogenic fetus and maternal leukocytes. Trophoblasts subvert maternal immunity by producing surface-bound and soluble factors that interact with maternal leukocytes. Here, we describe the distribution of three members of the expanding family of B7 immunomodulatory molecules: B7-DC, B7-H2, and B7-H3. B7-DC and B7-H3 inhibit antigen-stimulated lymphocyte activation while B7-H2 serves in a regulatory capacity, often promoting a Th2 immunophenotype. First trimester and term placentas, purified trophoblast cells, choriocarcinoma cell lines, and human umbilical vein endothelial cells were analyzed for B7 family RNA and protein expression. Transcripts and proteins for all three B7s were present throughout gestation but were differentially expressed within the trophoblast and the stroma. Whereas B7-DC was prominent on the syncytiotrophoblast of early placenta, it was absent from the trophoblast at term. In contrast, B7-H2 and B7-H3 were prominent on the extravillous trophoblast throughout gestation. Lastly, stromal cells, including macrophages and endothelial cells, differentially expressed B7-DC, B7-H2, and B7-H3, depending on gestational age. Thus, all three of these newly discovered B7 proteins are differentially positioned at the maternal-fetal interface such that they could steer maternal leukocytes away from a harmful immune response and toward a favorable one.  (+info)

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Purpose: Over the past four decades there have been minimal improvements in outcomes for patients with osteosarcoma (OS). New targets and novel therapies are needed to improve outcomes for these patients. In this study, we sought to evaluate the prevalence and prognostic utility of the immune checkpoint inhibitor HHLA2 in OS.. Experimental Design: HHLA2 expression was evaluated in two cohorts of OS patients using a tumor microarray (TMA) (n = 62) and whole slides (n = 48). HHLA2 expression was assessed in primary tumor specimens and metastatic disease, and correlated with the presence of tumor infiltrating lymphocytes (TILs), and event free survival.. Results: HHLA2 was expressed in 68% of OS tumors in the TMA and in 54% in the second cohort. HHLA2 was expressed in almost all metastatic disease specimens and was more prevalent than in primary specimens without known metastases (93% vs 53%, p = 0.02). TILs were present in 75% of all osteosarcoma specimens. Patients whose tumors were ≥25% or ...
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THE IgM B-CELL RECEPTOR a a THE IgM B-CELL RECEPTOR antigen binding mIg molecule H L V b a Ig-a/Ig-b heterodimer Signal transduction Lyn Kinases Syk Btk SHP-1 Phosphatases SLP-65/BLNK PLC HS1 Vav Adaptors + substrates
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A reminder that the next Vista Member Forum meeting is taking place on Saturday 30th January at the Vista Shop on Hotel Street, from 10am to 12.30pm. ...
With Win 98se I used an Iomega Parallel Port Zip 100 drive to store stuff on. Will Vista support this?? I need to use it long enough to transfer contents...
Microsoft is introducing small but significant modification to the Windows Vista End-User License Agreement (EULA) for Windows Anytime Upgrade. Customers who purchase a retail copy of Windows Vista and
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s question on why anti-virus software wont feature in Vista by saying the answer was complicated, but not based on technical concerns. Curiously, basic anti-spyware protection - via Windows Defender - will feature in Vista.. Vista will also include a major revamp of Internet Explorer (IE 7), features designed to thwart phishing, and group policy management features that make it easier to control the use of USB devices. Windows built-in firewall will be revamped to filter malicious traffic originating from a Vista PC as well as ingress filtering, the half on the equation that came with Windows XP.. Allchin said security in Vista is far improved from Windows XP SP2, whose heavily touted security features include technology that has made computer worms (though not Trojans based on the recent Windows Meta File vulnerability) less of a problem. SP2 was a very good system, but compared to Vista its night and day, Allchin said.. In a separate Q&A session, Allchin was grilled by Redmonds local ...
Hey Sitepoint. Im buying a new computer in early fabruary, after Vista is scheduled to be released. Tell us your opinions on which option you would take: Buy the computer with Vista installed Buy the computer with XP installed, and later upgrade to VistaPost your opinion here, and give some reasons, etc. How stable will Vista be? Will most software work (or not work) with Vista?... or at least cause problems? Would you wait for the first service pack to come out? Will there be
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Today we are going to discuss two new Vista performance enhancements, SuperFetch & ReadyBoost. We will also be discussing Vista RAM usage since these all work together. First, SuperFetch is an
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In the current study, we report a crucial role for TLT-1 in regulating leukocyte activation and modulating sepsis-induced acute inflammatory response. A 17-aa residue of its extracellular domain mediates this effect. Whereas treml-1−/− mice are particularly susceptible to infection with the development of an intense inflammatory state that translated into organ damage and rapid mortality, LR17 administration confers a strong protection.. Until recently, the role of TLT-1 has remained unclear. TLT-1 is exclusively expressed in platelets, colocalized with CD62P in α-granules of resting platelets. Upon activation, TLT-1 is quickly exposed on the membrane (24) and subsequently cleaved, leading to the release of a soluble fragment. Structural analysis of the extracellular domain of TLT-1 suggests the existence of several distinct potential ligand binding sites (27). The first TLT-1 ligand was recently identified to be fibrinogen, positioning TLT-1 as a regulator of hemostasis by facilitating ...
Coinhibitory receptor pathways have gained much attention as potential targets for immune modulation. Although a broad spectrum of pathologies that are caused or aggravated by dysregulated immune responses, such as cancer, autoimmunity, and chronic infection, can potentially be modulated by targeting coinhibitory receptor pathways, the development of such therapies is spearheaded by cancer therapy, where blockade of the coinhibitory receptors CTLA-4 and PD-1 has shown remarkable success (15). Despite these promising developments, low response rates, adverse autoimmune-like toxicity, and resistance highlight the need to identify additional pathways to broaden the therapeutic repertoire of coinhibitory receptors and overcome these hurdles. TIGIT represents such a potential therapeutic target; however, its mechanism of action has not been fully elucidated. In this study we have identified two monoclonal anti-TIGIT Abs that can be used to manipulate TIGIT function in vivo and investigate its ...
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Whats the last thing youre thinking of when you wander into a den of wild asps? …What system is best for running SolidWorks on 64 bit Vista. That does absolutely nothing for your survivability quotient.. Its a common misconception that SolidWorks doesnt run very well on 64 bit Vista. If you look into installing Microsoft Vista the experience can be quite the opposite. Now, people are using laptops and desktops loaded with Vista that kick the pants off XP systems.. However, it may help to know exactly what systems works best. So I turn it over to you, the reader.. Have you upgraded? What system do you use? What did you look for when upgrading your hardware? What programs didnt work? What did you pay? Tell us what your experience in the comments and Ill repost the findings.. ...
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Linsley PS, Brady W, Grosmaire L, Aruffo A, Damle NK, Ledbetter JA (March 1991). "Binding of the B cell activation antigen B7 ... March 1997). "B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation". ... Munn DH, Sharma MD, Mellor AL (April 2004). "Ligation of B7-1/B7-2 by human CD4+ T cells triggers indoleamine 2,3-dioxygenase ... To become activated, lymphocyte must engage both antigen and costimulatory ligand on the same antigen-presenting cell. T cell ...
B61 is a split antigen serotype that recognizes certain B40 serotypes. Ways JP, Lawlor DA, Wan AM, Parham P (1987). "A ... transposable epitope of HLA-B7, B40 molecules". Immunogenetics. 25 (5): 323-8. doi:10.1007/BF00404425. PMID 2437025. Marsh, S. ... Tissue Antigens. 75 (4): 291-455. doi:10.1111/j.1399-0039.2010.01466.x. PMC 2848993. PMID 20356336. Robinson J, Waller MJ, Fail ...
B7-H4 is an immune checkpoint molecule. B7 (protein) GRCh38: Ensembl release 89: ENSG00000134258 - Ensembl, May 2017 GRCm38: ... These proteins are expressed on the surface of antigen-presenting cells and interact with ligands (e.g., CD28; MIM 186760) on T ... "Genomic organization and expression analysis of B7-H4, an immune inhibitory molecule of the B7 family". Journal of Immunology. ... Kryczek I, Wei S, Zou L, Zhu G, Mottram P, Xu H, Chen L, Zou W (July 2006). "Cutting edge: induction of B7-H4 on APCs through ...
Association of the TCR of a naive T cell with MHC:antigen complex without CD28:B7 interaction results in a T cell that is ... Mouse CD Antigen Chart Human CD Antigen Chart Human CD28 genome location and CD28 gene details page in the UCSC Genome Browser ... B7-1 and B7-2 regulation of tyrosine kinase adaptor molecules". J. Biol. Chem. 271 (3): 1591-8. doi:10.1074/jbc.271.3.1591. ... Prasad KV, Cai YC, Raab M, Duckworth B, Cantley L, Shoelson SE, Rudd CE (Mar 1994). "T-cell antigen CD28 interacts with the ...
"The CD28/B7 pathway costimulates the response of primary murine T cells to superantigens as well as to conventional antigens". ... Rockefeller University News, June 16, 1996 Blankson, J.; Loh, D.; Morse, S. (1995). "Superantigens and conventional antigens ... antigens in HIV-1-infected patients with immune reconstitution". The Journal of Infectious Diseases. 183 (4): 657-661. doi: ...
Mao Y, Li W, Chen K, Xie Y, Liu Q, Yao M, Duan W, Zhou X, Liang R, Tao M (February 20, 2015). "B7-H1 and B7-H3 are independent ... CD27: This molecule supports antigen-specific expansion of naïve T cells and is vital for the generation of T cell memory. CD27 ... B7-H3's receptors have not yet been identified. B7-H4: also called VTCN1, is expressed by tumor cells and tumor-associated ... "B7-H3 is a potent inhibitor of human T-cell activation: No evidence for B7-H3 and TREML2 interaction". Eur J Immunol. 39 (7): ...
The class I region of these supertype is HLA A1-B8 haplotype, HLA A3-B7, HLA-A2-B7 and HLA A24-B7. Arce-Gomez B, Jones EA, ... HLA-Cw7 (Cw7) is a human leukocyte antigen serotype within HLA-C serotype group. (For more general information on HLA serotypes ... Cw7 in Europe is part of the AH8.1 and HLA B7-DR15-DQ6 haplotypes. ... Tissue Antigens. 11 (2): 96-112. doi:10.1111/j.1399-0039.1978.tb01233.x. PMID 77067. Allele Query Form IMGT/HLA - European ...
... a costimulatory signal is transmitted by the interaction between CD28 and B7 of the precursor cell and the licensed antigen- ... In the second phase, affector CTLs destroy target cells by recognizing the antigen-MHC class I complex. In phase one, effector ... This results in proliferation and differentiation of the antigen-activated precursor cell into a functional effector CTL. In ... This step allows the cell to become licensed to an antigen-presenting cell. Second, ...
A23 is a split antigen of the broad antigen HLA-A9 and it is a sister serotype of HLA-A24. A23 is common in Africa and regions ... A23-B7 is found in N.Afr. and S.Afr non-caucasians. A23-B44 is found in Albania, Zimbabwe, Morocco, Spain and Danes. A23-B58 is ... "The genetic control of HLA-A and B antigens in somatic cell hybrids: requirement for beta2 microglobulin". Tissue Antigens. 11 ... HLA-A23 (A23) is a human leukocyte antigen serotype within HLA-A serotype group. The serotype is determined by the antibody ...
Wang S, Bajorath J, Flies DB, Dong H, Honjo T, Chen L (May 2003). "Molecular modeling and functional mapping of B7-H1 and B7-DC ... Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubata T, Yagita H, Honjo T (May 1996). "Expression of the PD-1 antigen on the ... "Differential binding properties of B7-H1 and B7-DC to programmed death-1". Biochemical and Biophysical Research Communications ... PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 protein is upregulated on macrophages and ...
... also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA-4 binds CD80 and CD86 with greater affinity and ... work has suggested that CTLA-4 may function in vivo by capturing and removing B7-1 and B7-2 from the membranes of antigen- ... It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. The CTLA-4 protein is encoded ... CTLA-4 was first identified in 1991 as a second receptor for the T cell costimulation ligand B7 (Bashyam, 2007). In November ...
... blocks the binding of the antigen-presenting cell ligands B7.1 and B7.2 to CTLA-4, resulting in inhibition of B7- ... resulting in a B7-CD28-mediated T-cell activation unopposed by B7-CTLA-4-mediated inhibition. Unlike Ipilimumab (another fully ...
DCs are also the only cell type which can activate naïve T cells and induce antigen-specific immune responses. Therefore, their ... These molecules are: PD-L1, immunoglobulin like transcripts ILT (ILT3/4/5), B7-H1, SLAM, DEC-205. Tolerogenic effect has been ... Mills KH, McGuirk P (April 2004). "Antigen-specific regulatory T cells--their induction and role in infection". Seminars in ... Funda, David P.; Goliáš, Jaroslav; Hudcovic, Tomáš; Kozáková, Hana; Špíšek, Radek; Palová-Jelínková, Lenka (2018). "Antigen ...
Liu's laboratory demonstrated that induction of B7-1 and B7-2 explains the function of infection on adaptive immunity. As the ... His interest in this question was kindled when he made the observation that viral activation of host antigen-presenting cells ... induce co-stimulatory activity in antigen-presenting cells, and proposed that microbial induction of co-stimulatory molecules ...
MHC complex on a professional antigen-presenting cell and by the B7:CD28 costimulatory signal. Upon activation, "low-affinity" ...
In order for T cells to be activated and attack an antigen, that antigen must be presented to the T cell by an antigen- ... also known as B7-1 and B7-2). Abatacept binds to the CD80 and CD86 molecule, and prevents the second signal. Without the second ... For signal 2, the APC must present a B7 protein on its cell surface to a CD28 protein on the surface of the T cell. These two ... Abatacept prevents antigen-presenting cells (APCs) from delivering the co-stimulatory signal. This prevents the T cells from ...
It is closely related to CD86, another B7 protein (B7-2), and often works in tandem, binding to the same receptors to prime T ... If the interaction between an antigen-presenting cell and a T-cell is stable enough, the T-cell can remove the CD80 from the ... Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane protein that is in the immunoglobulin superfamily, ... "Expression of costimulatory molecules B7-1 (CD80), B7-2 (CD86), and interleukin 12 cytokine in multiple sclerosis lesions". The ...
April 2006). "B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma". The Journal of ... and cytotoxic T-lymphocyte antigen 4 (CTLA-4), respectively, on T cells. Inhibitory signals to PD-1 and CTLA-4 are immune ... in hepatocellular carcinoma and B7-homologs in ovarian carcinoma, which activate programmed cell death protein 1 (PD-1) ...
B7-H4), and various other phenotypic alterations that lead to immune escape (the loss of tumor antigens and other molecules ... a chimeric monoclonal antibody against the CD20 B-cell antigen, has therapeutic activity in diffuse large-B-cell lymphoma5. ... essential for antigen processing and presentation). Chemotherapy can promote tumor immunity in two major ways: (a) through its ...
Antigen-naive T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... which in turn outcompetes CD28 for binding to the B7 proteins. This is a checkpoint mechanism to prevent over activation of the ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help. Antigen ... These self-antigens are expressed by thymic cortical epithelial cells on MHC molecules on the surface of cortical epithelial ...
In malignant tissues, B7-H3 is an immune checkpoint molecule that inhibits tumor antigen-specific immune responses. B7-H3 also ... Cluster of Differentiation 276 (CD276) or B7 Homolog 3 (B7-H3) is a human protein encoded by the CD276 gene. B7-H3 is a 316 ... Petroff MG, Kharatyan E, Torry DS, Holets L (August 2005). "The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are ... 2Ig-B7-H3) or two identical pairs (4Ig-B7-H3) due to exon duplication. B7-H3 mRNA is expressed in most normal tissues. In ...
Antigen-naïve T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... which in turn outcompetes CD28 for binding to the B7 proteins. This is a checkpoint mechanism to prevent over activation of the ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help.[57] Antigen ... Antigen discrimination[edit]. A unique feature of T cells is their ability to discriminate between healthy and abnormal (e.g. ...
At that time it was concluded that B7-H1 helps tumor cells evade anti-tumor immunity. In 2003 B7-H1 was shown to be expressed ... In turn, clonal expansion of antigen-specific CD8+ T cells and/or CD4+ helper cells is propagated. The binding of PD-L1 to the ... Dong H, Zhu G, Tamada K, Chen L (December 1999). "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation ... PD-L1 also has an appreciable affinity for the costimulatory molecule CD80 (B7-1), but not CD86 (B7-2). CD80's affinity for PD- ...
Interaction with B7 on T cells Downregulation of CD80/CD86 costimultory molecules on antigen presenting cells upon interaction ... tumor antigens, alloantigens, and self-antigens in inflamed tissue. Immune recognition of non-self-antigens typically ... Self-antigens are present due to endogenous expression, importation of antigen from peripheral sites via circulating blood, and ... Upon exposure to a foreign antigen, either the antigen is eliminated by the standard immune response (resistance), or the ...
... one notable example includes a B7 ligand complex on antigen-presenting cells binding to the CD28 receptor on T cells); and ... Priming of antigen-specific naive lymphocytes occurs when antigen is presented to them in immunogenic form (capable of inducing ... Priming is the first contact that antigen-specific T helper cell precursors have with an antigen. It is essential to the T ... This activation of naive T cell is controlled by a variety of signals: recognition of antigen in the form of a peptide: MHC ...
... hla-a3 antigen MeSH D23.050.301.500.450.380 - hla-b antigens MeSH D23.050.301.500.450.380.383 - hla-b7 antigen MeSH D23.050. ... hla-a3 antigen MeSH D23.050.705.552.400.380 - hla-b antigens MeSH D23.050.705.552.400.380.383 - hla-b7 antigen MeSH D23.050. ... hla-a3 antigen MeSH D23.050.705.552.450.380 - hla-b antigens MeSH D23.050.705.552.450.380.383 - hla-b7 antigen MeSH D23.050. ... antigens, cd15 MeSH D23.101.100.900.131 - antigens, cd31 MeSH D23.101.100.920 - antigens, ly MeSH D23.101.100.930 - antigens, ...
Antigen Antigenicity Immunogen Superantigen Allergen Hapten Epitope Linear Conformational Mimotope Tumor antigen Antigen- ... Beta subunits B1 B2 B3 B4 B5 B6 B7 B8 Dimers Cytoadhesin receptor Integrin alpha6beta4 Glycoprotein IIb/IIIa - Heterodimer: ... T cells Antigen receptor - T cell receptor (TCR) Subunits - [email protected] / [email protected] / [email protected] / [email protected] Co-receptors CD8 (CD8α / CD8β) CD4 ... CD18 Macrophage-1 antigen (CR3) - Heterodimer: CD11b / CD18 Integrin alphaXbeta2 (CR4) - Heterodimer: CD11c / CD18 Very late ...
The antigen-presenting cell then travels to the lymph nodes and presents the antigen peptide and costimulatory molecule ... CTL responses can be enhanced by co-inoculation with co-stimulatory molecules such as B7-1 or B7-2 for DNA vaccines against ... This is true for intracellular and plasma membrane-bound antigens, but not for secreted antigens, which seem to generate TH2 ... Secreted or plasma membrane-bound antigens are more effective at inducing antibody responses than cytosolic antigens, while ...
A*02:Cw*16 is associated with increased higher viral load in HIV A2-B7 (Node in Netherlands) A2-B5 A2-B51 A2-B52 A2-B8 A2-B13 ... Arce-Gomez B, Jones EA, Barnstable CJ, Solomon E, Bodmer WF (Feb 1978). "The genetic control of HLA-A and B antigens in somatic ... HLA-A*02 (A*02) is a human leukocyte antigen serotype within the HLA-A serotype group. The serotype is determined by the ... ISBN 0-19-931641-4. Komlos L, Klein T, Korostishevsky M (Aug 2007). "HLA-A2 class I antigens in couples with recurrent ...
For terminology help see: HLA-serotype tutorial) B7, previously HL-A7, was one of the first 'HL-A' antigens recognized, largely ... B7 is found in two major haplotypes in Europe, where it reaches peak frequency in Ireland. One haplotype A3-B7-DR15-DQ1 can be ... B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.[citation needed] HLA-B7 along with ... HLA-B7 (B7) is an HLA-B serotype. The serotype identifies the more common HLA-B*07 gene products. ( ...
"Tissue Antigens. 64 (5): 575-80. doi:10.1111/j.1399-0039.2004.00310.x. PMID 15496200.. ... This compares with 25% of Cw7-B7 that extend to A3::DQ6 [4] Of 25 potential genetic recombinants of A1::DQ2, none exceed 10% of ... A1::DQ2 is not the longest haplotype, but the longest, HLA A3-Cw7-B7-DR15-DQ6 (A3::DQ6), had already undergone significant ... An A1::DQ2 appears in India, however its major antigen genes superficially resemble European A1-B8 and it appears to be a ...
MHC complex on a professional antigen-presenting cell and by the B7:CD28 costimulatory signal. Upon activation, "low-affinity" ...
Prostate specific membrane antigen is a transmembrane carboxypeptidase and exhibits folate hydrolase activity.[75] This protein ... Prostate cancer screening is controversial.[1][3] Prostate-specific antigen (PSA) testing increases cancer detection but does ... Although the widespread use of prostate-specific antigen (PSA) screening in the US has resulted in diagnosis at earlier age and ... These androgen-independent LNCaP cells have elevated AR expression and express prostate specific antigen upon androgen ...
B51 is a split antigen of the broad antigen B5, and is a sister serotype of B52.[2] There are a large number of alleles within ... Tissue Antigens. 61 (1): 20-48. doi:10.1034/j.1399-0039.2003.610103.x. PMID 12622774. Archived from the original (PDF) on 2008- ... "Tissue Antigens. 65 (4): 301-69. doi:10.1111/j.1399-0039.2005.00379.x. PMC 2396006. PMID 15787720.. .mw-parser-output cite. ... Ahn JK, Park YG (October 2007). "Human leukocyte antigen B27 and B51 double-positive Behçet uveitis". Arch. Ophthalmol. 125 (10 ...
The protein also carries the Jr(a) antigen, which defines the Junior blood group system.[9] ...
antigen. -. Broad. antigen. Split antigens B7. B5. B51. B52. B8. B12. B44. B45. ... antigen processing and presentation. • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP- ... antigen processing and presentation of peptide antigen via MHC class I. • regulation of dendritic cell differentiation. • ... antigen processing and presentation of endogenous peptide antigen via MHC class Ib. • innate immune response. • defense ...
Antigen-specific peripheral shaping of the natural regulatory T cell population. „J Exp Med". 205 (13), s. 3105-3117, grudzień ... B7/CD28 costimulation is essential for the homeostasis of the CD4+CD25+ immunoregulatory T cells that control autoimmune ... De novo production of antigen-specific suppressor cells in vivo. „Nat Protoc". 1 (2), s. 653-661, 2006. PMID: 17802642. ... B7/CD28 in central tolerance: costimulation promotes maturation of regulatory T cell precursors and prevents their clonal ...
A*03:01 ; C*07:02 ; B*07:02 ; DRB1*15:01 ; DQA1*01:02 ; DQB1*06:02 (By serotyping A3-Cw7-B7-DR15-DQ6 or the older version "A3- ... HLAs corresponding to MHC class II (DP, DM, DOA, DOB, DQ, and DR) present antigens from outside of the cell to T-lymphocytes. ... List of human leukocyte antigen alleles associated with cutaneous conditions. References[edit]. *^ Galbraith W, Wagner MC, Chao ... which in turn stimulate antibody-producing B-cells to produce antibodies to that specific antigen. Self-antigens are suppressed ...
"Entrez Gene: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)".. *^ May, K. E.; Villar, J.; Kirtley, S.; ... CD61+Antigens at the US National Library of Medicine Medical Subject Headings (MeSH) ...
doi:10.1212/01.wnl.0000144279.21502.b7.. *↑ Burt TD, Agan BK, Marconi VC, He W, Kulkarni H, Mold JE, Cavrois M, Huang Y, Mahley ... "Apolipoprotein-mediated pathways of lipid antigen presentation". Nature 437 (7060): 906-10. PMID 16208376. doi:10.1038/ ...
antigen processing and presentation of exogenous peptide antigen via MHC class II. • proteolysis. • neutrophil degranulation. ... doi:10.1023/B:CLIN.0000024740.44602.b7. PMID 15168727.. *. Liaudet-Coopman E, Beaujouin M, Derocq D, Garcia M, Glondu-Lassis M ... brain antigen processing and regulation of programmed cell death.[17][18][19][20] ...
Antigen-naïve T cells expand and differentiate into memory and effector T cells after they encounter their cognate antigen ... which in turn outcompetes CD28 for binding to the B7 proteins. This is a checkpoint mechanism to prevent over activation of the ... T cell exhaustion can be triggered by several factors like persistent antigen exposure and lack of CD4 T cell help.[51] Antigen ... Antigen discriminationEdit. A unique feature of T cells is their ability to discriminate between healthy and abnormal (e.g. ...
"Antigens recognized by autologous antibody in patients with renal-cell carcinoma". International Journal of Cancer. 83 (4): 456 ... "A conserved domain of the Epstein-Barr virus nuclear antigens 3A and 3C binds to a discrete domain of Jkappa". Journal of ...
The positive B cell signaling is initiated by binding of foreign antigen to surface immunoglobulin. The same antigen-specific ... B7 family. Immunol Rev 241, 180-205 10.1111/j.1600-065X.2011.01011.x ... IgE antibodies bind to antigens of allergens. These allergen-bound IgE molecules interact with Fcε receptors on the surface of ... When an appropriate allergic antigen or parasite is present, the cross-linking of a least two of IgE molecules and their Fc ...
Antigen i +. {\displaystyle +}. A. 1. +. {\displaystyle A1^{+}}. a. =. 376. {\displaystyle a=376}. b. =. 237. {\displaystyle b= ... B7 0.039 10.3 A2 Bw40 0.013 4.4 A2 Bw15 0.01 3.4 A1 Bw17 0.014 5.4 ... frequency of antigen i. {\displaystyle i}. : p. f. i. =. C. N. =. 0.311. ;. {\displaystyle pf_{i}={\frac {C}{N}}=0.311;}. ... frequency of antigen j. {\displaystyle j}. : p. f. j. =. A. N. =. 0.237. ;. {\displaystyle pf_{j}={\frac {A}{N}}=0.237;}. ...
ജൈവരസതന്ത്രം, ഔഷധശാസ്ത്രം എന്നിവയിൽ, ഒരു കോശത്തിന് പുറത്തുനിന്നുള്ള രാസ അടയാളങ്ങൾ ലഭിക്കുന്ന ഒരു പ്രോട്ടീൻ തന്മാത്രയാണ് റിസെപ്റ്റർ.[1]അത്തരം രാസ അടയാളങ്ങൾ റിസപ്റ്ററിലേക്ക് ബന്ധിപ്പിക്കുമ്പോൾ അവ ചില സെല്ലുലാർ / ടിഷ്യുകൾക്കുള്ള പ്രതികരണമായി മാറുന്നു. ഉദാ. ഒരു കോശത്തിന്റെ വൈദ്യുത പ്രവർത്തനത്തിലെ ഒരു മാറ്റം. റിസീപ്റ്ററിന്റെ പ്രവർത്തനം വർഗ്ഗീകരിക്കാൻ മൂന്ന് പ്രധാന ...
প্রতিজন বা প্রত্যুৎপাদক (Antigen). *শ্লৈষ্মিক ঝিল্লি (Mucus membrane). *শ্লেষ্মা (Mucus). *কেরাটিন বা শৃঙ্গপদার্থ (Keratin) ...
Interaction with B7 on T cells. *Downregulation of CD80/CD86 costimultory molecules on antigen presenting cells upon ... Self-antigens are present due to endogenous expression, importation of antigen from peripheral sites via circulating blood, and ... Upon exposure to a foreign antigen, either the antigen is eliminated by the standard immune response (resistance), or the ... nTreg cells are specific, modestly, for self-antigen while iTreg cells recognize allergens, commensal bacteria, tumor antigens ...
First Antigen Rapid Test for Ebola through Emergency Assessment and Eligible for Procurement»։ World Health Organization։ ...
Gaudet R, Wiley DC (2001). „Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen ...
Complete stimulation of T helper cells requires the B7 molecule present on the antigen presenting cell to bind with CD28 ... Steps in production of antibodies by B cells: 1. Antigen is recognized and engulfed by B cell 2. Antigen is processed 3. ... Antigen presentation[edit]. Main articles: antigen presentation and major histocompatibility complex. After the processed ... Antigens can be large and complex substances, and any single antibody can only bind to a small, specific area on the antigen. ...
In biochemistry and pharmacology, a receptor is a protein molecule that receives chemical signals from outside a cell.[1] When such chemical signals bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. There are three main ways the action of the receptor can be classified: relay of signal, amplification, or integration.[2] Relaying sends the signal onward, amplification increases the effect of a single ligand, and integration allows the signal to be incorporated into another biochemical pathway.[2] In this sense, a receptor is a protein-molecule that recognizes and responds to endogenous chemical signals, e.g. an acetylcholine receptor recognizes and responds to its endogenous ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug targets, such as enzymes, transporters, and ion channels.. Receptor proteins can be classified by their location. Transmembrane ...
... in addition to the ABO antigens and Rh antigens, many other antigens are expressed on the RBC surface membrane. For example, an ... The most significant Rh antigen is the D antigen, because it is the most likely to provoke an immune system response of the ... Dean, Laura (2005). Blood Groups and Red Cell Antigens, a guide to the differences in our blood types that complicate blood ... and also possible sensitization to Rh D antigens. One exception is individuals with hh antigen system (also known as the Bombay ...
"Transcriptional regulation of A33 antigen expression by gut-enriched Krüppel-like factor". Oncogene. 22 (28): 4434-43. doi ...
Every helper T-cell is specific to one particular antigen. Only professional antigen-presenting cells (macrophages, B ... An example of this includes the interaction of the membrane proteins of the B7 family of the dendritic cell with CD28 present ... Their main function is to process antigen material and present it on the cell surface to the T cells of the immune system. They ... Here they act as antigen-presenting cells: they activate helper T-cells and killer T-cells as well as B-cells by presenting ...
"The role of B7 costimulation in T-cell immunity." Immunology and cell biology 77, no. 4 (1999): 304. "Professor Franca Ronchese ... Hilligan KL, Ronchese F (2020). Antigen presentation by dendritic cells and their instruction of CD4+ T helper cell responses. ... where she became interested in antigen presentation by dendritic cells in vivo. In 1994 Franca moved to New Zealand to ...
One B7-DR2-DQ6 derives from the fact that DR15 is a split antigen of the DR2 broad antigen serotype. The other B7-DR2-DQ1 stems ... The appendation of the B7::DQ6 haplotype creates the A2-B7::DQ6 haplotype. This haplotype if found often in Northern Spain, ... HLA B7-DR15-DQ6 is a representation(by serotype) of a common HLA haplotype found in Western Eurasia. The haplotype can be ... HLA B7-DR15-DQ6 was found to have an association with postmenopausal osteoporosis in a Greek population. DR2 (15 or 16) -DQ6.2 ...
CD4-dependent host protection is B7-1/B7-2 independent after Nb inoculation. B7-1/B7-2+/+ and B7-1/B7-2−/− BALB/c mice (five ... BALB/c mice compared with B7-1/B7-2+/+ BALB/c mice after Nb inoculation. a, B7-1/B7-2+/+ and B7-1/B7-2−/− BALB/c mice were bled ... rather than using B7-1/B7-2-deficient mice, CTLA4Ig was used to block B7-1/B7-2 interactions in WT B7-1/B7-1+/+ BALB/c mice in ... To examine whether the host protective Th2 response was intact in mice deficient in B7-1 and B7-2, B7-1/B7-2−/− and B7-1/B7-2 ...
Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation.. P S ... Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. ... Here we show that CD28 is the primary receptor for B7 on activated peripheral blood T cells, that CD28 binds to B7 in the ... B7 Ig also inhibited CD28-mediated cellular adhesion. The function of CD28-B7 interactions during T cell activation was ...
Activation B7-1 Antigen or CTLA 4 Counter Receptor B7. ... T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen ... T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) - Pipeline Review, H2 ... T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) - Cluster of ... T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) pipeline Target ...
Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) - Pipeline ... Enquire before buying for T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86 ... Enquire before buying T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) - ... T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) - Pipeline Review, H1 ...
... on antigen-presenting cells are not encoded by the B7 gene. CTLA4 ligand A also differs from B7 in cellular distribution and in ... A major costimulatory molecule on antigen-presenting cells, CTLA4 ligand A, is distinct from B7. Y Wu Y Wu ... Y Wu, Y Guo, Y Liu; A major costimulatory molecule on antigen-presenting cells, CTLA4 ligand A, is distinct from B7.. J Exp Med ... Cytotoxic T Lymphocyte Antigen 4 (CTLA4) Blockade Accelerates the Acute Rejection of Cardiac Allografts in CD28-deficient Mice ...
T1 - Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 1. Isolation and Amino Acid ... Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 1. Isolation and Amino Acid ... Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 1. Isolation and Amino Acid ... Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. 1. Isolation and Amino Acid ...
We then used this model to evaluate the antitumor activity of CAR T cells targeting B7-H3, an antigen that is expressed in a ... an antigen that is expressed in a broad range of solid tumors including OS. B7-H3-CAR T cells had potent antitumor activity in ... B7-H3-CAR T cells had potent antitumor activity in a dose-dependent manner and inhibited the development of pulmonary ... In contrast T cells expressing an inactive B7-H3-CAR had no antitumor activity. Using unmodified LM7 cells also enabled us to ...
The remarkable functional plasticity of professional antigen-presenting cells (APCs) allows the adaptive immune system to ... B7 Antigens / metabolism* * Bystander Effect* * CTLA-4 Antigen / metabolism* * Dendritic Cells / metabolism* ... CTLA-4(+) microvesicles can competitively bind B7 costimulatory molecules on bystander DC, resulting in downregulation of B7 ... The remarkable functional plasticity of professional antigen-presenting cells (APCs) allows the adaptive immune system to ...
Buy B7-H3 antibodies and other products for immunotherapy research here at ProSci Inc.! These antibodies are guaranteed to work ... Additional Antibody B7-H3 Products. Monoclonal antibodies are highly specific and recognize only one epitope on an antigen ... B7-H3 Antibody: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its ... Risk-Free B7-H3 Set (RF16090). 20μg / Clone. RF16091, RF16092, RF16093, RF16094, RF16095. Mouse monoclonal B7-H3 antibodies ...
Human TGF-α Capture Bead B7, 13X - LEGENDplex™ Human Growth Factor Mix and Match Panel Capture Beads are intended for use with ... Antigen Details Biology Area Cell Biology, Signal Transduction, Stem Cells Molecular Family Growth Factors, Cytokines/ ...
Capture Bead B7, 13X - LEGENDplex™ Mouse Proinflammatory Chemokine Mix and Match Panel Capture Beads are intended for use with ... Antigen Details Biology Area Immunology, Innate Immunity Molecular Family Cytokines/Chemokines Gene ID 20311 View all products ...
p,LEGENDplex™ Human TFF3 Capture Bead B7, 13X is suitable for use in the following Mix and Match Panels:,br /,,br /,,u,Human ... Antigen Details Molecular Family Enzymes and Regulators Gene ID 7033 View all products for this Gene ID UniProt View ... LEGENDplex™ Human TFF3 Capture Bead B7, 13X is suitable for use in the following Mix and Match Panels:. Human Kidney Function ...
B7-H3.BB.z-chimeric antigen receptor (CAR) T cells administered intracerebroventricularly or intratumorally mediate potent ... B7-H3.BB.z-CAR T cells administered intracerebroventricularly or intravenously mediate antigen-specific protection from tumor ... These results identify B7-H3 as a compelling therapeutic target for this largely incurable pediatric tumor and demonstrate ... Consistent with the embryonic origin of ATRT5,6, B7-H3 is highly expressed on the prenatal, but not postnatal, brain. ...
Browse our B7-H4 Peptides and Proteins all backed by our Guarantee+. ... B7-H4 Peptides and Proteins available through Novus Biologicals. ... Recombinant Protein Antigen (1). clear. Has Sample Size. *. No ... Our B7-H4 Peptides and B7-H4 Proteins can be used in a variety of model species: Human. Use the list below to choose the B7-H4 ... B7-H4 Proteins. We offer B7-H4 Peptides and B7-H4 Proteins for use in common research applications: Blocking/Neutralizing, ...
Expression of the immunosuppressive protein B7 homolog 1 (B7-H1), also known as programmed death ligand-1 (PD-L1), is increased ... Antigens, CD / genetics* * Antigens, CD / immunology * Antigens, CD / metabolism * B7-H1 Antigen ... Expression of the immunosuppressive protein B7 homolog 1 (B7-H1), also known as programmed death ligand-1 (PD-L1), is increased ... Loss of tumor suppressor PTEN function increases B7-H1 expression and immunoresistance in glioma Nat Med. 2007 Jan;13(1):84-8. ...
For terminology help see: HLA-serotype tutorial) B7, previously HL-A7, was one of the first HL-A antigens recognized, largely ... B7 is found in two major haplotypes in Europe, where it reaches peak frequency in Ireland. One haplotype A3-B7-DR15-DQ1 can be ... B7 is a risk factor for cervical cancer, sarcoidosis, and early-onset spondylarthropathies.[citation needed] HLA-B7 along with ... HLA-B7 (B7) is an HLA-B serotype. The serotype identifies the more common HLA-B*07 gene products. ( ...
One B7-DR2-DQ6 derives from the fact that DR15 is a split antigen of the DR2 broad antigen serotype. The other B7-DR2-DQ1 stems ... The appendation of the B7::DQ6 haplotype creates the A2-B7::DQ6 haplotype. This haplotype if found often in Northern Spain, ... HLA B7-DR15-DQ6 is a representation(by serotype) of a common HLA haplotype found in Western Eurasia. The haplotype can be ... HLA B7-DR15-DQ6 was found to have an association with postmenopausal osteoporosis in a Greek population. DR2 (15 or 16) -DQ6.2 ...
A CAR T-cell therapy targeting the B7-H3 protein may have potential as a treatment for several childhood cancers, results from ... But the B7-H3 CAR T cells target an antigen that is found in many non-blood cancers (i.e., solid tumors), including some ... B7-H3: A "Pan-Cancer Antigen" for Childhood Cancers?. With this new study, the research team began by performing what they ... Both target an antigen known as CD19. A third CAR T cell that targets an antigen called BCMA is expected to be approved later ...
HLA class I histocompatibility antigen, B-7 alpha chain-like. * Clones. (1 products) View Products ...
B-Lymphocyte Activation Antigen B7-2 (LAB7-2) Polyclonal Antibody B-Lymphocyte Activation Antigen B7-2 (LAB7-2) Polyclonal ... To order B-Lymphocyte Activation Antigen B7-2 (LAB7-2) Polyclonal Antibody , please use the Cat. Nr. CAU27021 and submit your ... B-Lymphocyte Activation Antigen B7-2 (LAB7-2) Polyclonal Antibody should be stored at -20°C ... Our specialists recommend you to follow carefully the pre-registered instructions for B-Lymphocyte Activation Antigen B7-2 ( ...
ELISA Kit for B-Lymphocyte Activation Antigen B7-2 (LAB7-2) - Human ... Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7 ... This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-2 (LAB7-2). ... Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7- ...
CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2 ... Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native ... 100ug-Anti-B-Lymphocyte Activation Antigen B7-2 (LAB7-2) 100ug-Anti-B-Lymphocyte Activation Antigen B7-2 (LAB7-2). ...
B7-H4, a newly identified B7 homolog, plays a key role in maintaining T-cell homeostasis by reducing T-cell proliferation and ... More importantly, B7-H4 plays an indispensable role in alloimmunity in the absence of the classic CD28/CTLA-4 : B7 pathway, ... B7-H4 protects allograft survival and generates donor-specific tolerance. It also prevents the development of autoimmune ... The relationship between B7-H4 and allograft survival/autoimmunity has been investigated recently in both islet transplantation ...
Mouse Monoclonal Antibody Shop B7-H7/HHLA2 Mouse anti-Human, Alexa Fluor 350, Clone: 907812R, ... B7-H7/HHLA2 Mouse anti-Human, Alexa Fluor 350, Clone: 907812R, R&D Systems™- ... Antigen. B7-H7/HHLA2. Clone. 907812R. Dilution. Flow Cytometry 0.25-1 ug/10^6 cells. ... B7-H7/HHLA2 Monoclonal antibody specifically detects B7-H7/HHLA2 in Human samples. It is validated for Flow Cytometry ...
B7-1) Armenian Hamster anti-Canine, Mouse, Porcine, PE, Clone: 16-10A1, 200 µg; PE. ... In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 ... CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 ( ... CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, ...
The past years have witnessed significant advance in our understanding of critical roles of T cell co-signals in B7-CD28 family ... 0/Antigens, CD; 0/Antigens, CD28; 0/Antigens, CD80; 0/Antigens, CD86; 0/Antigens, Differentiation; 0/Antigens, Differentiation ... Antigens, CD. Antigens, CD28 / immunology*. Antigens, CD80 / immunology*. Antigens, CD86 / immunology*. Antigens, ... Antigens, Differentiation, T-Lymphocyte / immunology. Humans. Lymphocyte Activation. Receptors, Immunologic / immunology. ...
... pSP65 containing a genomic human B7 gene (gB7). ... J27-B7 is a stable transfected cell line established in 1987 by ... Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: ... J27-B7 expresses surface HLA B7 but does not secrete HLA B7. ... J27-B7 expresses surface HLA B7 but does not secrete HLA B7. ... J27-B7 is a stable transfected cell line established in 1987 by transfection with calcium phosphate of the J27.2 cell line with ...
The HLA type of IMT was HLA-A1, 2; B7, 8; DR3, 4 (DRB1*0301, 1401); DQ1, 2. Donor 6947 was a normal healthy donor with the HLA ... The concerted action of a Th and a CTL response against the same mutant antigen could provide a particularly efficient form of ... The following antibodies were used in blocking experiments: B7/21, anti-HLA-DP (a gift of F. Bach, University of Minneapolis, ... In general, genetically altered proteins provide a source of tumor-specific peptides that can result in antitumor antigen T ...
PD-L1, B7-1, and B7-2 Are Coexpressed on Antigen-presenting Cells.. We next examined the pattern of PD-L1 expression on antigen ... 3 B). B7-1 and B7-2 mRNAs were also induced upon activation: B7-1 was induced 21-fold at 4 h and 22-fold at 20 h. B7-2 showed ... constitutive expression of B7-1 and B7-2 is not seen in any organ. B7-1 and B7-2 expression can be induced in dendritic cells, ... PD-1 is structurally similar to CTL-associated antigen 4 (CTLA-4), which binds B7-1 and B7-2 and plays a crucial role for the ...
B7-H1 Antigen. Grant support. *U54 CA163125/CA/NCI NIH HHS/United States ... Programmed cell death ligand 1 (PD-L1) is an immunomodulatory molecule expressed by antigen-presenting cells and select tumors ...
  • T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) - Cluster of differentiation 80 or CD80 or B7-1 is a protein found on dendritic cells, activated B cells and monocytes. (reportbuyer.com)
  • T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) pipeline Target constitutes close to 10 molecules. (reportbuyer.com)
  • The latest report T Lymphocyte Activation Antigen CD80 - Pipeline Review, H2 2018, outlays comprehensive information on the T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (reportbuyer.com)
  • It also reviews key players involved in T Lymphocyte Activation Antigen CD80 (Activation B7-1 Antigen or CTLA 4 Counter Receptor B7.1 or CD80) targeted therapeutics development with respective active and dormant or discontinued projects. (reportbuyer.com)
  • Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. (fishersci.com)
  • CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). (fishersci.com)
  • CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). (fishersci.com)
  • To determine whether B7 signals are associated with atopic responses in man, we assayed CD80 and CD86 expression on B cells and monocytes from atopic patients and controls. (elsevier.com)
  • Peripheral blood mononuclear cells from 10 patients with perennial allergic rhinitis and from 10 normal subjects were cultured in the presence or absence of house-dust-mite antigen, and B cells and monocytes were assayed for expression of CD80 and CD86 by flow cytometry. (elsevier.com)
  • It is important to note that PD-L1 also interacts with CD80 (B7-1), which is expressed on the surface of CD8 + T cells. (frontiersin.org)
  • Binding of CD28 on the T cell to B7-1 (CD80) and B7-2 (CD86) molecules on the antigen-presenting cell creates an amplifying signal required for full T cell activation. (jci.org)
  • Background: CD80 (B7-1) and CD86 (B7-2) play an important role in antigen presentation to effector cells. (elsevier.com)
  • Methods: We assayed the expression of CD80 and CD86 on allergen-specific T-cell lines from patients with perennial allergic rhinitis stimulated by Dermatophagoides farinae-crude (Der f-c) antigen, 1 of the major allergens causing house dust mite allergy. (elsevier.com)
  • Results: On T-cell activation, CD86 antigen was upregulated earlier than CD80. (elsevier.com)
  • Conversely, antigen-specific Th1 and Th2 clones were insensitive to treatment with either anti-CD80, anti-CD86, or a combination of the two. (elsevier.com)
  • Abatacept binds CD80 and CD86 on antigen presenting cells (APCs), blocking interaction with CD28 on T lymphocytes, which initiates a co-stimulatory signal required for full activation of T lymphocytes. (cancer.gov)
  • CD80 dimers on the antigen presenting cells (APCs) bridge CTLA4/CD152 dimers on T-cells in a periodic zipper-like arrangement. (genecards.org)
  • The CD86 antigen (B7-2, B70) is a single-chain trans-membrane glycoprotein, structurally similar to CD80. (beckman.com)
  • The extracellular architecture of CTLA-4 is characterized by a single IgV-like domain containing the B7-1 (CD80)/B7-2 (CD86) ligand-binding site. (thermofisher.com)
  • The CD80 antigen (B7, BB1) is a highly glycosylated single-chain protein. (beckman.com)
  • CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. (beckman.com)
  • Global Markets Direct's, 'T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) - Pipeline Review, H1 2017', provides in depth analysis on T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) targeted pipeline therapeutics. (globalmarketsdirect.com)
  • T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86)Additionally, the report provides an overview of key players involved in T Lymphocyte Activation Antigen CD86 (Activation B7-2 Antigen or CTLA 4 Counter Receptor B7.2 or CD86) targeted therapeutics development and features dormant and discontinued projects. (globalmarketsdirect.com)
  • Our results seem to suggest that selective upregulation of CD86 on B cells by a challenging antigen may play a critical role in the development of Th2 cells in patients with atopic disease. (elsevier.com)
  • In addition, we compared the proportion of CD45RO + CD86 + T cells in primary culture of PBMCs stimulated by Der f-c antigen between patients with perennial allergic rhinitis and control subjects. (elsevier.com)
  • We conclude that CD86 is the primary B7 signaling homologue in human PBMC responses, and that second signal pathways through the B7 homologues have no effect on phenotypically differentiated T helper cells in humans. (elsevier.com)
  • On lymphocytes, CD86 appears as a B-cell activation antigen. (beckman.com)
  • The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. (antibodies-online.com)
  • Rat CD86 / B7-2 derived in Human Cells. (creativebiomart.net)
  • Th2 immune responses to a number of infectious pathogens are dependent on B7-1/B7-2 costimulatory molecule interactions. (jimmunol.org)
  • Many protective Th2 responses to infectious pathogens require B7-1/B7-2 costimulatory molecule interactions for maturation of naive CD4 + T cells into IL-4-producing Th effector cells. (jimmunol.org)
  • A major costimulatory molecule on antigen-presenting cells, CTLA4 ligand A, is distinct from B7. (rupress.org)
  • They provide the basis for the formal alignment of the acid cleavage and cyanogen bromide fragments of the molecule as well as the source material for the elucidation of the primary structure of the HLA-B7 heavy chain. (umn.edu)
  • Programmed cell death ligand 1 (PD-L1) is an immunomodulatory molecule expressed by antigen-presenting cells and select tumors that engages receptors on T cells to inhibit T-cell immunity. (nih.gov)
  • Cytotoxic T lymphocyte antigen-4 (CTLA-4) is a regulatory molecule that suppresses T cell effector function following initial activation by costimulatory signals. (jci.org)
  • Differential modulation of this key immunoinhibitory molecule in tumor versus normal tissues may advance both cell-mediated immunotherapy and antibody-based targeted strategies using the B7-H3-specific mAb 8H9. (aacrjournals.org)
  • however, only liver pDCs significantly upregulate expression of the coregulatory molecule B7 homolog-1 (B7-H1) in response to IL-27. (jimmunol.org)
  • Several investigators have suggested that the interaction of the CD28 molecule on the T cell with a ligand, B7, on the antigen-presenting cell (APC) is best characterized among the many cell surface receptor/ligand pairs in delivering this costimulatory activity. (google.com)
  • Here, we found a strong association between melanocyte expression of B7-H1 (PD-L1), an immune-inhibitory molecule, and the presence of tumor-infiltrating lymphocytes (TILs) in human melanocytic lesions: 98% of B7-H1 + tumors were associated with TILs compared with only 28% of B7-H1 − tumors. (sciencemag.org)
  • Indeed, the co-inhibitory molecule B7-H1 (also called PD-L1), which is often up-regulated on tumor cells, impairs T cell responses-leading to anergy, exhaustion, or apoptosis upon engagement with its cognate co-inhibitory receptor PD-1, which is often highly expressed on tumor-infiltrating lymphocytes (TILs) ( 3 - 8 ). (sciencemag.org)
  • Co-Stimulatory Molecule B7-2. (invivogen.com)
  • Here we show that CD28 is the primary receptor for B7 on activated peripheral blood T cells, that CD28 binds to B7 in the absence of other accessory molecules, and that interaction between CD28 and B7 is costimulatory for T cell activation. (rupress.org)
  • CTLA-4(+) microvesicles can competitively bind B7 costimulatory molecules on bystander DC, resulting in downregulation of B7 surface expression with significant functional consequences for downstream CD8(+) T-cell responses. (nih.gov)
  • B7-H3 Antibody: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). (prosci-inc.com)
  • The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. (fishersci.com)
  • The past years have witnessed significant advance in our understanding of critical roles of T cell co-signals in B7-CD28 family molecules in regulating T cell activation and tolerance. (biomedsearch.com)
  • 21 . A method according to any one of claims 1 - 20 wherein the antigen is administered in combination with at least one member selected from the group consisting of cytokines, lymphokines, co-stimulatory molecules, and nucleic acids coding therefor. (google.com)
  • We have pursued this hypothesis by searching for B7-like molecules and testing their binding to PD-1. (rupress.org)
  • High level of HLA I and II molecules were expressed in most human blood malignant cell lines examined, and the co-stimulatory factor B7-2 was also highly expressed. (bvsalud.org)
  • B7-H1 encompasses a recently discovered cell surface glycoprotein within the B7 family of T-cell coregulatory molecules. (aacrjournals.org)
  • C07K14/70532 - B7 molecules, e.g. (google.com)
  • Antigen-specific T cell activation depends on T cell receptor (TCR) interaction with peptide/major histocompatibility complex (MHC) in conjunction with co-stimulatory signals mediated by accessory molecules. (google.com)
  • The B7 family molecules have been shown to regulate immune responses in both positive and negative fashions. (aacrjournals.org)
  • The aim of this study was to examine whether leukemic cells of acute myeloid leukemia express functional B7 family molecules and, if so, whether such expression has any clinical significance. (aacrjournals.org)
  • The expression of four B7 family molecules, B7.1, B7.2, B7-H1, and B7-H2, on leukemic cells from acute myeloid leukemia patients was analyzed by flow cytometry. (aacrjournals.org)
  • Although B7.1 and B7-H1 expressions were rare, the cells from a substantial number of acute myeloid leukemia cases expressed B7.2 and B7-H2 molecules [mean percentages of B7.2- and B7-H2-positive cells were 28.9% ( n = 58) and 15.3% ( n = 59), respectively]. (aacrjournals.org)
  • Our results indicate the expression of functional B7.2 and B7-H2 molecules, and these molecules may facilitate progression of acute myeloid leukemia. (aacrjournals.org)
  • The interaction between B7 molecules [i.e. (aacrjournals.org)
  • CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. (thermofisher.com)
  • Peptides presented by human leukocyte antigen (HLA) molecules on the cell surface play a crucial role in adaptive immunology, mediating the communication between T cells and antigen presenting cells. (mcponline.org)
  • Antigens binding to class I molecules are 8-10 amino acids long and play an important role in recognition of the virus infected and malignant cells by cytotoxic T lymphocytes (CTLs). (miltenyibiotec.com)
  • Stimulation of TCR is triggered by MHC (Major Histocompatibility Complex) molecules on antigen presenting cells that present antigen peptides to TCR complexes and induce a series of intracellular signaling cascades that regulate T-cell development, homeostasis, activation, acquisition of effector's functions and apoptosis. (thermofisher.com)
  • Costimulation involves an integration of activating signals and inhibitory signals from CD28 and CTLA4 (Cytotoxic T-Lymphocyte Antigen-4) molecules, respectively, with TCR signals to determine the outcome of a T-cell's encounter with an antigen (Ref. 2). (thermofisher.com)
  • Such immature DC effectively endocytose antigens, although they express the accessory molecules needed for T cell activation at low levels. (springer.com)
  • Foreign antigens, including allergens or pathogens, that enter the body are taken up by so-called antigen-presenting cells (APC), which process the antigens and present peptides, thereof, in the context of major histocompatibility complex class (MHC) II molecules on their cell surface. (ersjournals.com)
  • PsA is associated with specific major histocompatibility complex class I molecules that are involved in antigen presentation to T cells, which are implicated in disease pathogenesis. (bmj.com)
  • Costimulatory B7 molecules (e.g. (abnova.com)
  • Here, we developed a novel spontaneously metastasizing orthotopic OS model and explored its utility to evaluate the efficacy of chimeric antigen receptor (CAR) T cells. (frontiersin.org)
  • One is that their target antigen must sit on the surface of the cancer cell, where the T cell's genetically engineered receptor-the chimeric antigen receptor , or CAR-can bind to it. (cancer.gov)
  • B7-H3.BB.z-chimeric antigen receptor (CAR) T cells administered intracerebroventricularly or intratumorally mediate potent antitumor effects against cerebral ATRT xenografts in mice, with faster kinetics, greater potency and reduced systemic levels of inflammatory cytokines compared to CAR T cells administered intravenously. (nature.com)
  • Antitumor responses in the absence of toxicity in solid tumors by targeting B7-H3 via chimeric antigen receptor T cells. (nature.com)
  • T cell activation is initiated when antigen is presented to the T cell receptor (TCR) complex by MHC class I or II on an antigen-presenting cell. (jci.org)
  • B7-H3 preferentially down-regulated T H 1-mediated immune response in B7-H3-deficient mice ( 5 ), and 4Ig-B7-H3 inhibited natural killer (NK) cell-mediated lysis of neuroblastoma cells by interacting with a putative inhibitory receptor on the surface of NK cells ( 6 ). (aacrjournals.org)
  • for example, human B7-H3 (2Ig form) promoted T-cell activation and IFN-γ production by binding to a putative receptor on activated T cells ( 2 ). (aacrjournals.org)
  • B7-DC (CD273) has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. (novusbio.com)
  • B7-DC (CD273) has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. (novusbio.com)
  • The receptor(s) for B7-H3 remains unknown. (rndsystems.com)
  • The inhibition may govern through nuclear factor of activated T cells (NFAT), NF‑ kappa B, and AP-1 factors, three major signaling pathways through which TCR regulates gene transcription, which suggesting that B7‑H3 might have more than one receptor on T cells (6). (rndsystems.com)
  • Optimal activation of T cells requires second costimulatory signals together with the first signal delivered by engagement of the T-cell receptor with peptide-MHC complex on antigen-presenting cells (APC). (aacrjournals.org)
  • Ipilimumab, a blocking monoclonal antibody (mAb) directed against the co-inhibitory T cell receptor CTLA-4 (cytotoxic T lymphocyte-associated antigen 4), systemically activates T cells, which led to enhancement of antitumor T cell responses and demonstrated a survival benefit in patients with advanced metastatic melanoma in randomized trials ( 10 , 11 ). (sciencemag.org)
  • In the periphery, one important level of regulation is the action of costimulatory signals in concert with TCR (T-Cell antigen Receptor) signals to promote full T-cell activation (Ref. 1). (thermofisher.com)
  • During his fellowship, he cared for some of the first pediatric patients to receive CD19 chimeric antigen receptor (CAR) T cells, children with B cell acute lymphoblastic leukemia (B-ALL) who often had no other therapeutic option. (stanford.edu)
  • This phase I trial studies the best dose and side effects of CD19/CD22 chimeric antigen receptor (CAR) T cells when given together with chemotherapy, and to see how well they work in treating children or young adults with CD19 positive B acute lymphoblastic leukemia that has come back or does not respond to treatment. (stanford.edu)
  • As CTLA4 immunoglobulin (CTLA4Ig) binds B7 when the latter is expressed on fibroblasts, it was widely assumed that CTLA4Ig blocks T cell costimulation by blocking the function of B7. (rupress.org)
  • The invention relates generally to compositions of and methods for obtaining and using a polypeptide, other than B7, that binds to CTLA4, or CD28, or CTLA4Ig, or homologous proteins, and regulates T cell activation. (google.com)
  • This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • Ig (binds antigen) -- IgM and IgD are 1st. (brainscape.com)
  • Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ B7-H3 antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. (prosci-inc.com)
  • Mouse monoclonal B7-H3 antibodies were raised against the extracellular domain of human B7-H3. (prosci-inc.com)
  • ProSci Risk-Free™ B7-H3 antibodies are rigorously tested and guaranteed to work for their designated applications. (prosci-inc.com)
  • Monoclonal antibodies are highly specific and recognize only one epitope on an antigen which generates consistent performance in quantitative detection applications regardless of production batch. (prosci-inc.com)
  • Polyclonal antibodies have high affinity and recognize different epitopes on a specific antigen. (prosci-inc.com)
  • PeproTech's polyclonal antibodies are purified through the isolation of specific polyclonal antibodies from antiserum by antigen affinity chromatography. (peprotech.com)
  • The programmed death-1 (PD-1) and its ligand PD-L1 (B7-H1) signaling pathway has been the focus of much enthusiasm in the fields of tumor immunology and oncology with recent FDA approval of the anti-PD-1 antibodies pembrolizumab and nivolumab and the anti-PD-L1 antibodies durvalumab, atezolimuab, and avelumab. (frontiersin.org)
  • Consistent with this finding, acute myeloid leukemia cells expressing B7.2 and B7-H2 induced allogeneic CD4 + T cells to proliferate and secrete interleukin-4 and interleukin-10 in vitro , effects that were partially blocked by antibodies against B7.2 and B7-H2. (aacrjournals.org)
  • The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): Functional effects of subunit-specific monoclonal antibodies. (springer.com)
  • Human peripheral blood cells after erythrocyte lysis were stained with Anti-HLA-B7, B27 antibodies or with the corresponding REA Control (S) antibodies (left images). (miltenyibiotec.com)
  • PD-L1 is expressed by antigen-presenting cells, including human peripheral blood monocytes stimulated with interferon γ, and activated human and murine dendritic cells. (rupress.org)
  • Programmed death-ligand 1 (PD-L1, also referred to as B7-H1 or CD274) is constitutively expressed by cells of the myeloid lineage, including macrophages and dendritic cells ( 1 - 3 ). (frontiersin.org)
  • Inflammatory cytokines, such as IFN gamma, and a combination of phorbol myristate acetate (PMA) and ionomycin induce expression of B7-H3 protein on dendritic cells (DCs) and monocytes (4). (rndsystems.com)
  • CD28 is a cell surface glycoprotein constitutively expressed on most T cells, which has been recently shown to interact with B7, which is expressed on dendritic cells, macrophages, and activated B and T cells. (google.com)
  • B7-H1 expression is detected not only on APCs, such as macrophages, B cells, and dendritic cells, but also on activated T cells and some tumor cells, such as lung and colon cancers ( 4 , 5 , 10 ). (aacrjournals.org)
  • One major defect involves inefficient antigen presentation by tumor-infiltrating dendritic cells and macrophages ( 2 ). (pnas.org)
  • Diverse vaccination strategies, however, may enhance the acquisition and processing of tumor antigens by dendritic cells and thereby engender protective immunity ( 3 ). (pnas.org)
  • Dendritic cells fused with human cancer cells: morphology, antigen expression, and T-cell stimulation. (army.mil)
  • However, it was not known that this protein is present and upregulated on the surface of tumor associated dendritic cells until Dr. Zou's laboratory discovered that B7-H1 expression is increased in dendritic cells under tumor environment factors. (army.mil)
  • Soluble and membrane-bound factors produced in the tumor microenvironment impede the activation of these professional antigen-presenting cells, skewing host reactions toward immunosuppressive circuits and dysfunctional effector T cells. (pnas.org)
  • Expression of the immunosuppressive protein B7 homolog 1 (B7-H1), also known as programmed death ligand-1 (PD-L1), is increased in many pathological conditions, including cancer. (nih.gov)
  • Here we show that expression of the gene encoding B7-H1 increases post transcriptionally in human glioma after loss of phosphatase and tensin homolog (PTEN) and activation of the phosphatidylinositol-3-OH kinase (PI(3)K) pathway. (nih.gov)
  • B7-H4, a newly identified B7 homolog, plays a key role in maintaining T-cell homeostasis by reducing T-cell proliferation and cytokine production. (hindawi.com)
  • Cynomolgus Monkey B7 homolog 3 (B7-H3), also known as CD276 antigen, is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1-3). (rndsystems.com)
  • Note: B7-H3 antibody should be stored in working aliquots at -20 °C, stable for up to one year. (prosci-inc.com)
  • To order B-Lymphocyte Activation Antigen B7-2 (LAB7-2) Polyclonal Antibody , please use the Cat. (anigenetics.com)
  • B7-H7/HHLA2 Monoclonal antibody specifically detects B7-H7/HHLA2 in Human samples. (fishersci.com)
  • Therefore, the use of the superior antigen affinity-purified antibody preparations can help alleviate background in these analytical procedures. (peprotech.com)
  • The monoclonal antibody (mAb) 8H9 is specific for 4Ig-B7-H3, the long and principal form of B7-H3. (aacrjournals.org)
  • In contrast, in vivo monoclonal antibody-mediated blockade of B7-H1 has been shown to potentiate antitumoral responses in several murine cancer models. (aacrjournals.org)
  • Recombinant Cynomolgus Monkey B7‑H3 inhibits IFN-gamma secretion by human T cells in the presence of anti-CD3 antibody. (rndsystems.com)
  • The following product was used in this experiment: B7-H3 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-82384, RRID AB_2789543. (thermofisher.com)
  • Humanization of an anti-lymphocyte function-associated antigen (LFA)-1 monoclonal antibody and reengineering of the humanized antibody for binding to rhesus LFA-1. (springer.com)
  • Additionally, Dr. Zou and colleagues demonstrated that expression of myeloid DC-B7-H1 could be blocked with specific monoclonal antibody that could activate T-cell function and decrease the production of T cell IL-10. (army.mil)
  • The mouse monoclonal antibody BB7.1 recognizes an extracellular antigen of HLA-B7 antigen. (exbio.cz)
  • These results demonstrate that the CD28 signaling pathway could be activated by B7, resulting in increased T cell cytokine production and T cell proliferation. (rupress.org)
  • Potent control of acute graft-versus-host disease lethality after immunization with a novel DNA vaccine targeted to B7-1/CD28 costimulatory signaling pathway. (semanticscholar.org)
  • We show that ATRTs robustly express B7-H3 / CD276 that does not result from the inactivating mutations in SMARCB1 (refs. (nature.com)
  • Human B7-H3 (also named as CD276) is a member of the B7/CD28 immunoglobulin superfamily, which provides crucial costimulatory signals that regulate T-cell functions in tumor surveillance, infections, and autoimmune diseases ( 1 ). (aacrjournals.org)
  • We recently showed that CD28, a T cell surface protein that regulates an activation pathway, could mediate intercellular adhesion with activated B cells by interaction with the B7 antigen. (rupress.org)
  • As a part of the overall strategy for determining the complete covalent structure of the papain-solubilized portion of the heavy chain of the human histocompatibility antigen H.LA-B7, the protein was dissected into various fragments by a combination of partial acid hydrolysis and cyanogen bromide cleavage. (umn.edu)
  • Cytotoxic T-lymphocyte-associated protein-4 (CTLA-4), the target of the blockbuster cancer immunotherapeutic ipilimumab, is one of the most well-known and well-studied members of the B7 superfamily and negatively regulates T cell responses by a variety of known mechanisms. (nih.gov)
  • The CAR T cells, designed to bind to a protein called B7-H3 on the surface of cancer cells, eradicated tumors in mouse models of several different childhood cancers , including two forms of sarcoma and medulloblastoma . (cancer.gov)
  • One of the most promising aspects of this particular CAR T-cell therapy is that it targets a single protein, or antigen, that is present at high levels in numerous cancers. (cancer.gov)
  • Each B7-H4 Peptide and B7-H4 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Tumor specimens from individuals with glioblastoma multiforme (GBM) had levels of B7-H1 protein that correlated with PTEN loss, and tumor-specific T cells lysed human glioma targets expressing wild-type PTEN more effectively than those expressing mutant PTEN. (nih.gov)
  • 5%), which cannot be effectively isolated by standard purification procedures (e.g. ion exchange chromatography) or by non-antigen-specific affinity procedures, such as Protein A/G affinity purification. (peprotech.com)
  • 4 . A method according to any one of claims 1 to 3 wherein the antigen is a protein. (google.com)
  • MAbs and ligands are known to be the most specific agents to an antigen or protein expressed on cancer cells, while toxins are the most potent agent killing cancer cells. (pubmedcentralcanada.ca)
  • A soluble fusion protein consisting of the extracellular domain of human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) linked to a modified Fc (hinge, CH2, and CH3 domains) portion of human immunoglobulin G1 (IgG1) with immunosuppressive activity. (cancer.gov)
  • Whereas B7-H3 transcript was ubiquitously expressed in a wide spectrum of human solid tumors as well as human normal tissues, B7-H3 protein was preferentially expressed only in tumor tissues. (aacrjournals.org)
  • B7-H3 protein expression was inversely correlated with miR-29 levels in both cell lines and tumor tissues tested. (aacrjournals.org)
  • Using luciferase reporter assay, miR-29a was shown to directly target B7-H3 3′ untranslated region, and knock-in and knockdown of miR-29a led to down-regulation and up-regulation, respectively, of B7-H3 protein expression. (aacrjournals.org)
  • The ability of miR-29 to control B7-H3 protein expression has implications in immune escape by solid tumors. (aacrjournals.org)
  • Moreover, gene expression studies have shown many nonstandard, unexpected protein products, including the production of antigens derived from aberrant protein fragments as a result of expression in alternative reading frames ( 6 ). (mcponline.org)
  • The activation of T-cells by Antigen-MHC-II complex carried on antigen presenting cells is a complex process involving a cascade of events, the first of which is phosphorylation of the PTKs (Protein Tyrosine Kinases) belonging to the Src and SYK ZAP70 (Zeta-Chain-Associated Protein Kinase) families. (thermofisher.com)
  • Furthermore, studies extended on immune deficient mice bearing human tumors also showed that blockade of myeloid DC-B7-H1 protein could activate a T-cell mediated immune response. (army.mil)
  • 6 Abatacept, a selective T-cell costimulation modulator, is a soluble fusion protein comprising the extracellular domain of human cytotoxic T-lymphocyte-associated antigen-4 linked to the modified Fc (hinge, CH2 and CH3 domains) portion of human immunoglobulin G1. (bmj.com)
  • We then used this model to evaluate the antitumor activity of CAR T cells targeting B7-H3, an antigen that is expressed in a broad range of solid tumors including OS. (frontiersin.org)
  • But the B7-H3 CAR T cells target an antigen that is found in many non-blood cancers (i.e., solid tumors), including some pediatric cancers. (cancer.gov)
  • There are very few [antigens] on the [tumor cell] surface and that are unique to tumors," explained Marcela Maus, M.D., Ph.D., director of Cellular Immunotherapy at the Massachusetts General Hospital Cancer Center, at a December 2018 NCI-sponsored workshop on cell-based immunotherapies. (cancer.gov)
  • CAR T cells targeting B7-H3, a pan-cancer antigen, demonstrate potent preclinical activity against pediatric solid tumors and brain tumors. (nature.com)
  • In addition, some human tumors acquire the ability to aberrantly express B7-H1. (aacrjournals.org)
  • In addition, we have shown that B7-H1 expression by clear cell RCC tumors (or infiltrating mononuclear cells) correlates with aggressive pathologic features, including advanced tumor-node-metastasis stage, tumor size, higher nuclear grade, and coagulative necrosis. (aacrjournals.org)
  • Five-year cancer-specific survival rates in this study were 42% and 83% for patients harboring B7-H1 + versus B7-H1 − RCC tumors, respectively. (aacrjournals.org)
  • Therefore, the B7-H2-ICOS signal may favor the induction of Th2-type responses and thus may be involved in the negative regulation of cell-mediated immune responses against tumors. (aacrjournals.org)
  • Interferon-γ, a primary inducer of B7-H1 expression, was detected at the interface of B7-H1 + tumors and TILs, whereas none was found in B7-H1 − tumors. (sciencemag.org)
  • These observations suggest that therapies that block this pathway may benefit patients with B7-H1 + tumors. (sciencemag.org)
  • He has generated and optimized novel receptors to recognize antigens over-expressed on pediatric solid tumors such as GD2 (Mount/Majzner et al. (stanford.edu)
  • We offer B7-H4 Peptides and B7-H4 Proteins for use in common research applications: Blocking/Neutralizing, Control, SDS-Page. (novusbio.com)
  • Our B7-H4 Peptides and B7-H4 Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our B7-H4 Peptides and Proteins. (novusbio.com)
  • Antigens are peptides or recombinant or native dependent on the production method. (anigenetics.com)
  • Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method. (anigenetics.com)
  • Here we show that the major costimulatory ligand bound by CTLA4Ig (which we term CTLA4 ligand A) on antigen-presenting cells are not encoded by the B7 gene. (rupress.org)
  • A more recent study looked at a number of linked gene-alleles and found I82-2:D6S265-1:HLA-A3:D6S128-2:HLA-F1:D6S105-8 was constantly associated while B7 appeared beyond the haplotype linked to disease. (wikipedia.org)
  • J27-B7 is a stable transfected cell line established in 1987 by transfection with calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65 containing a genomic human B7 gene (gB7). (atcc.org)
  • 9. The method of anyone of claims 1 - 3 , wherein the gene encodes an antigen. (google.com)
  • We report here that the ligand of PD-1 ( PD-L1 ) is a member of the B7 gene family. (rupress.org)
  • Transfecting B7-1 gene to B7-1 negative (B7(-)) Raji and B7(-) Jurkat cell lines by liposome , and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transfection using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). (bvsalud.org)
  • Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potency for activation of anti- tumor T cells . (bvsalud.org)
  • Rini BI, Selk LM, Vogelzang NJ: Phase I study of direct intralesional gene transfer of HLA-B7 into metastatic renal carcinoma lesions. (exbio.cz)
  • We have previously shown that adenoviral gene transfer of B7-I/IL-2 to murine breast cancer induces a high rate of complete turnout regression and systemic immunity. (diva-portal.org)
  • We also observe the effects of B7-H4 gene expression on cell proliferation and migration in the human NSCLC cell line. (ersjournals.com)
  • Compare to cell lines without treatment, the proliferation of cell lines cultivated with decreased B7-H4 gene was increased, the cell cycle was blocked in the G 2 as well, invasion and mobility abilities of cells in vitro were added. (ersjournals.com)
  • Our observations also suggest that the B7-H4 gene is associated with A549 cell proliferation, migration and cell cycle distribution. (ersjournals.com)
  • RNAi recombinent of B7-H4 gene could effectively inhibit the expression of B7-H4 mRNA in A549 cells. (ersjournals.com)
  • B7-H3 was reported to act as a co-stimulatory regulator to enhance the proliferation of both CD4 + and CD8 + T cells, the induction of cytotoxic T cells, and IFN-gamma production in the presence of TCR signaling (1). (rndsystems.com)
  • Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) functions as a negative regulator of endogenous and vaccine-induced antitumor immunity. (pnas.org)
  • In this context, a key role has emerged for cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) as a negative regulator of antitumor responses ( 5 ). (pnas.org)
  • These cells, in turn, need to recognize and react to these antigens and mature to cytotoxic cells that destroy the tumor. (springer.com)
  • 125I-labeled B7 Ig bound to CD28-transfected Chinese hamster ovary (CHO) cells, and to immobilized CD28 Ig with a Kd approximately 200 nM. (rupress.org)
  • The function of CD28-B7 interactions during T cell activation was investigated with soluble fusion proteins and with B7-transfected CHO cells. (rupress.org)
  • Immobilized B7 Ig and B7+ CHO cells costimulated T cell proliferation. (rupress.org)
  • Cellular interactions mediated by B7 and CD28 may represent an important component of the functional interactions between T and B lymphoid cells. (rupress.org)
  • B7-H3-CAR T cells had potent antitumor activity in a dose-dependent manner and inhibited the development of pulmonary metastases resulting in a significant survival advantage. (frontiersin.org)
  • In contrast T cells expressing an inactive B7-H3-CAR had no antitumor activity. (frontiersin.org)
  • Using unmodified LM7 cells also enabled us to demonstrate that B7-H3-CAR T cells traffic to orthotopic tumor sites. (frontiersin.org)
  • The data so far on B7-H3 CAR T cells are encouraging, said Stephen Gottschalk, M.D., chair of the Department of Bone Marrow Transplantation and Cellular Therapy at St. Jude Children's Research Hospital in Tennessee, who was not involved in the research. (cancer.gov)
  • If the target antigen is present on healthy cells as well, the CAR T cells could attack those cells. (cancer.gov)
  • However, as Dr. Maus pointed out, the antigen targets of the two FDA-approved CAR T-cell therapies, and the therapy moving toward approval, are also expressed on some types of normal cells. (cancer.gov)
  • And, indeed, their analysis showed that B7-H3 was present on the surface of cancer cells in many of the tumor samples, including samples of neuroblastoma , Wilms tumor , and a particularly difficult-to-treat form of brain cancer called DIPG . (cancer.gov)
  • following clearance of ATRT xenografts, B7-H3.BB.z-CAR T cells administered intracerebroventricularly or intravenously mediate antigen-specific protection from tumor rechallenge, both in the brain and periphery. (nature.com)
  • These results identify B7-H3 as a compelling therapeutic target for this largely incurable pediatric tumor and demonstrate important advantages of locoregional compared to systemic delivery of CAR T cells for the treatment of CNS malignancies. (nature.com)
  • Fig. 2: Locoregional administration of B7-H3 CAR T cells is more potent and results in lower systemic inflammatory cytokine levels than IV administration against aggressive orthotopic ATRT xenografts. (nature.com)
  • Fig. 3: Locoregionally administered B7-H3 CAR T cells home more rapidly to cerebral ATRT xenografts than those administered intravenously. (nature.com)
  • B7-H3 CAR T cells administered intracerebroventricularly efficiently migrate out of the CNS. (nature.com)
  • Fig. 4: B7-H3 CAR T cells persist in the brain and lead to antigen-specific protection from tumor rechallenge. (nature.com)
  • These frameshift sequences only occur in tumor cells and their premalignant progenitors, and may accordingly constitute a previously unknown group of potential tumor specific antigens. (pnas.org)
  • In addition, activated T cells express this antigen. (fishersci.com)
  • Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. (atcc.org)
  • The relative levels of inhibitory PD-L1 and costimulatory B7-1/B7-2 signals on antigen-presenting cells may determine the extent of T cell activation and consequently the threshold between tolerance and autoimmunity. (rupress.org)
  • however, both murine and human T cells express high levels of PD-L1 upon antigen stimulation ( 3 , 5 ). (frontiersin.org)
  • Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti- tumor T cell activation, which was demonstrated by up regulation of expression of T cell cytokines IL-2 , IL-4 and INF-gamma mRNAs after incubation of these tumor cells with T cells for 24 h. (bvsalud.org)
  • Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoietic malignancies . (bvsalud.org)
  • B7-H3, a surface immunomodulatory glycoprotein, inhibits natural killer cells and T cells. (aacrjournals.org)
  • Liver pDCs treated with IL-27 increase the percentage of CD4 + Foxp3 + T cells in MLR, which is dependent upon expression of B7-H1. (jimmunol.org)
  • B7-H1 expression can be induced on activated T lymphocytes and is normally expressed by macrophage lineage cells. (aacrjournals.org)
  • B7-H3 mRNA is found in various normal tissues and in several tumor cell lines, but is not detectable in peripheral blood mononuclear cells (PBMCs). (rndsystems.com)
  • Both murine and human B7-H3 fusion proteins fail to bind to resting T cells, but can recognize activated T cells which stimulated with PHA or ConA, indicating that B7-H3 receptors are induced upon T cell activation (5). (rndsystems.com)
  • Blocking the binding of CD28 on T cells to its ligand, B7/BB-1, during TCR engagement, results in T cell anergy. (google.com)
  • 25% of leukemic cells expressed B7-H2 had significantly shorter survival, and this B7-H2 positivity had the strongest prognostic value when B7-H2 and other prognostic factors were analyzed together by multivariate analysis ( P = 0.0108). (aacrjournals.org)
  • B7-H1 inhibits the proliferation of T cells through a counterreceptor, programmed death-1 (PD-1) ( 8 ). (aacrjournals.org)
  • B7-H1 also delivers a stimulatory signal to T cells through a yet unidentified counterreceptor other than PD-1 ( 9 ). (aacrjournals.org)
  • We reported that B7-H1 on tumor cells suppressed antitumor immunity by inhibiting proliferation of tumor-specific CTL ( 10 - 12 ). (aacrjournals.org)
  • B7-H2 is expressed on professional APCs and tumor cells, such as glioma and gastric carcinoma ( 13 - 16 ). (aacrjournals.org)
  • The counterreceptor of B7-H2, inducible costimulator (ICOS), is expressed on activated T cells ( 17 ). (aacrjournals.org)
  • The B7-H2-ICOS signal induces T cells to proliferate and to secrete both Th1 and Th2 cytokines, such as interleukin (IL)-4 and IFN-γ, but not the potent Th1 cytokine, IL-2. (aacrjournals.org)
  • However, it has not yet been reported that B7-H2 expression on tumor cells is associated with such a negative regulation, but it has been reported that the B7-H2-ICOS interaction augments CTL expansion in mice transplanted with a potent immunogenic, MHC class I-positive tumor ( 20 ). (aacrjournals.org)
  • Although many human cancers such as melanoma express tumor antigens recognized by T cells, host immune responses often fail to control tumor growth for as yet unexplained reasons. (sciencemag.org)
  • Therefore, induction of the B7-H1/PD-1 pathway may represent an adaptive immune resistance mechanism exerted by tumor cells in response to endogenous antitumor activity and may explain how melanomas escape immune destruction despite endogenous antitumor immune responses. (sciencemag.org)
  • These T cells recognize tumor-associated or tumor-specific antigens ( 1 ). (sciencemag.org)
  • B7-deficient autoreactive T cells are highly susceptible to suppression by CD4(+)CD25(+) regulatory T cells. (semanticscholar.org)
  • Since Tregs constitutively express CTLA-4 and activated T cells express B7-1 and B7-2, it has been suggested that the interaction between CTLA-4 on Tregs and B7-1/2 on the effector T cells may be required for immune suppression. (semanticscholar.org)
  • For a DC-based tumor therapy to be successful, DCs from cancer patients must be able to take up tumor-specific antigens and present these in an immunostimulatory fashion to immunologically competent T cells. (springer.com)
  • B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. (creative-biolabs.com)
  • DCs are antigen-presenting cells in the immune system. (army.mil)
  • These consist of naturally occurring CD25+ Treg cells and adaptive Treg cells that are postulated to prevent immune responses against self-antigens and adaptive immune responses, respectively. (ersjournals.com)
  • Once activated, the Th-cells orchestrate adaptive antigen-specific cell-mediated and humoral immune responses. (ersjournals.com)
  • The antigen is also expressed by HTLV-1 transformed T cells and activated monocytes. (beckman.com)
  • Current work focuses on imparting multi-specificity to CAR T cells and optimizing these receptors to enhance their efficacy when the amount of target (antigen density) is limiting (Majzner et al. (stanford.edu)
  • MIM 601020) ligands on antigen-presenting cells. (genetex.com)
  • D ENDRITIC CELLS (DC), as the most potent antigen-presenting cells, play a central role in antitumor immunity. (bloodjournal.org)
  • B7-H4 shRNA was cloned into pGCsi-U6/Neo plasmid and the product was transfected into A549 cells with Lipofectamine 2000. (ersjournals.com)
  • After transfected with pGC shB7-H4, the expression of B7-H4mRNA in A549 cells was obviously decreased with an increased cell proliferation observably. (ersjournals.com)
  • CTLA4 ligand A also differs from B7 in cellular distribution and in the respective levels of expression. (rupress.org)
  • Fig. 1: High levels of B7-H3 expression in ATRTs and during normal prenatal brain development. (nature.com)
  • In more recent studies of patients with prostate cancer, tumor B7-H3 expression was strongly correlated with disease spread at time of surgery, increased risk of clinical cancer recurrence, and cancer-specific death ( 7 , 8 ). (aacrjournals.org)
  • Moreover, tumor B7-H3 expression was correlated with patient poor survival in both clear cell renal cell carcinoma and urothelial cell carcinoma ( 9 , 10 ). (aacrjournals.org)
  • Furthermore, antitumor response was enhanced by B7-H3 expression in murine tumor models ( 11 ). (aacrjournals.org)
  • Inhibition of STAT3 activation completely abrogates IL-27-induced upregulation of B7-H1 expression on liver pDCs. (jimmunol.org)
  • In one study of 306 patients, with a median clinical follow-up of 11 years, we reported that RCC B7-H1 expression correlates with increased risk of disease progression, cancer-specific death, and overall mortality even after multivariate adjustment. (aacrjournals.org)
  • The act of the APC ingesting antigen and breaking it down stimulates the expression of B7. (brainscape.com)
  • B7 C0-stimulation expression? (brainscape.com)
  • 2013) Co-expression of HLA-B7 and HLA-B27 alleles is associated with B7-restricted immunodominant responses following influenza infection. (miltenyibiotec.com)
  • B7-H1/TIL colocalization was identified not only in melanomas but also in inflamed benign nevi, indicating that B7-H1 expression may represent a host response to tissue inflammation. (sciencemag.org)
  • Therefore, TILs may actually trigger their own inhibition by secreting cytokines that drive tumor B7-H1 expression. (sciencemag.org)
  • Expression of CTLA4 is dependent both on TCR stimulation by the antigens and CD28-B7 engagement. (thermofisher.com)
  • In contrast to CTLA4-mediated sequestration of B7, negative signaling by CTLA4 requires the cytoplasmic tail of CTLA4 and is operational at low levels of surface expression (Ref. 4). (thermofisher.com)
  • Dr. Zou and his team demonstrated that tumor environmental factors impaired myeloid DC-mediated T cell activation by upregulating B7-H1 expression on myeloid DCs. (army.mil)
  • B7-H4 expression was evaluated by immunohistochemistry in 102 patients with NSCLC who underwent surgical tumor resection. (ersjournals.com)
  • In tumor tissues, expression of B7-H4 is found both in the cell membrane and in the cytoplasm. (ersjournals.com)
  • Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed "STEAP" (Six Transmembrane Epithelial Antigen of the Prostate). (freepatentsonline.com)
  • B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C-like domains with a short cytoplasmic tail. (rndsystems.com)
  • Much is known about the proteins involved in antigen processing, but high fidelity ligand/epitope predictions are at present not possible. (mcponline.org)
  • This technology eliminates the need to isolate specific tumor antigens (the proteins that can trigger an immune response) as the original tumor is utilized in the fusion. (army.mil)
  • Both B7 and CTLA4 ligand A are critically involved in T cell costimulation. (rupress.org)
  • At the cell membrane, CTLA4 undergoes dimerization, and each CTLA4 dimer can bind two independent B7-1/B7-2 homodimers, forming a linear zipper-like structure between B7-1/B7-2 and CTLA-4 homodimers (Ref. 6 & 7). (thermofisher.com)
  • One mechanism involves antagonism of B7-CD28-mediated costimulatory signals by CTLA4, which occurs because CTLA4 has a much higher affinity for B7 than does CD28. (thermofisher.com)
  • This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-2 (LAB7-2). (biotecnika.org)
  • B7-H4, a member of the B7 family, is involved in the regulation of antigen-specific immune responses. (ersjournals.com)
  • It is very possible that B7-H3 has both coinhibitory and costimulatory properties depending on the receptors ( 13 ). (aacrjournals.org)
  • To characterize the binding of CD28 to B7, we have produced genetic fusions of the extracellular portions of B7 and CD28, and immunoglobulin (Ig) C gamma 1 chains. (rupress.org)
  • Subsequently, a second dominantly expressed form of human B7-H3 that contains tandemly duplicated V-like and C-like immunoglobulin domain (4Ig-B7-H3) was found ( 3 , 4 ). (aacrjournals.org)
  • Clone REA176 recognizes HLA B7 and B27 class I human leukocyte antigens (HLA). (miltenyibiotec.com)
  • Complete Amino Acid Sequence of a Papain-Solubilized Human Histocompatibility Antigen, HLA-B7. (umn.edu)
  • Sarcoidosis and human leukocyte antigen class I and II genes: it takes two to tango? (wikipedia.org)
  • HLA B7-DR15-DQ6 is a multigene haplotype that covers a majority of the human major histocompatibility complex on chromosome 6. (wikipedia.org)
  • Secretion of genetically engineered human/mouse class I antigens. (atcc.org)
  • A BLAST search of the human expressed sequence tag (EST) database of the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov) identified two overlapping ESTs with homology to B7-1 and B7-2 (AA292201 and AA399416). (rupress.org)
  • To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti- tumor T cell immune responses . (bvsalud.org)
  • Examining human leucocyte antigen (HLA) and B7 expressions on 8 human blood malignancies cell lines by flow cytometry . (bvsalud.org)
  • The present study explores this hypothesis, using human PBMCs and antigen-specific Th1 and Th2 clones. (elsevier.com)
  • The inhibitory role of B7-H3 was supported by the reports that both 2Ig and 4Ig forms of human B7-H3 inhibited T-cell proliferation and cytokine production ( 3 ). (aacrjournals.org)
  • Human carcinoembryonic antigen (CEA) is a well-characterized oncofetal glycoprotein whose overexpression by human carcinomas has been a target for cancer immunotherapy. (aacrjournals.org)
  • Recombinant Human PD-L2/B7-DC Fc C. (novusbio.com)
  • Cynomolgus monkey B7-H3 shares approximately 97% and 88% aa sequence identity with human and mouse B7-H3 respectively. (rndsystems.com)
  • A human lymphocyte-associated antigen involved in cell-mediated lympholysis. (springer.com)
  • B7-H1 is now known to be present in human cancers including ovarian cancer. (army.mil)
  • Human leukocyte antigens (HLA) have been found to be associated with the pathogenesis of autoimmune diseases, allergies and inflammatory bowel diseases, and there are emerging evidences of correlations between HLA genotypes and renal diseases such as diabetic nephropathy, IgA nephropathy, and glomerulonephritis. (biomedcentral.com)
  • Tested positive against native human antigen. (creativebiomart.net)
  • Tumor-associated B7-H1, as well as B7-H1 on activated lymphocytes, has been shown to impair antigen-specific T-cell function and survival in vitro . (aacrjournals.org)
  • One of its responses to a foreign antigen is the proliferation of a class of lymphocytes which specifically recognizes the antigen. (google.com)
  • Upon engagement by B7-1 or B7-2, CTLA-4 expressed on the surface of activated T lymphocytes signals for cell-cycle arrest and attenuation of effector function ( 6 - 8 ). (pnas.org)
  • Both target an antigen known as CD19. (cancer.gov)
  • With several of these pathogens, inhibition of the IL-4-associated response by B7 blockade is associated with deviation to a Th1 immune response associated with IFN-γ elevations, suggesting that the Th2 response is more dependent on B7 ligand interactions than the Th1 response ( 5 , 6 , 8 ). (jimmunol.org)
  • 1 . A method of enhancing an immune response to an antigen in an animal comprising (a) administering an effective amount of an inducing agent to the animal followed by (b) administering an effective amount of the inducing agent and the antigen to the animal. (google.com)
  • The results demonstrate the ability to generate an effective antitumor immune response to a tumor self-antigen by immunization with a recombinant vaccinia virus. (aacrjournals.org)
  • Since oestrogens not only affect breast cancer but also have been shown to modulate immune function and secretion of immune-regulatory cytokines, we explored whether administration of oestradiol altered the immune response induced by an adenoviral vector expressing B7-I/IL-2. (diva-portal.org)
  • B7-DC is also called programmed death ligand 2 (PDL2). (novusbio.com)
  • B7 co-stimulation plays an important role in anti- tumor immunity . (bvsalud.org)
  • Consequently, tumor-associated B7-H1 has garnered much attention in the recent literature as a potential inhibitor of host antitumoral immunity. (aacrjournals.org)
  • Such associations may relate to the recognized ability of B7-H1 to inhibit T-cell-mediated antitumoral immunity. (aacrjournals.org)
  • The T-cell compartment of adaptive immunity provides vertebrates with the potential to survey for and respond specifically to an incredible diversity of antigens. (thermofisher.com)
  • 2003. Blockade of B7-H1 improves myeloid dendritic cell-mediated antitumor immunity. (army.mil)
  • B7‑H3 was also reported to play an inhibitory role on T-cell activation. (rndsystems.com)
  • however, in marked contrast, B cell Ag-specific Ab production is abrogated after B7 blockade. (jimmunol.org)
  • Dr. Zou's research provides evidences that ovarian cancer growth can be slowed down significantly in the presence of B7-H1 blockade. (army.mil)
  • More importantly, B7-H4 plays an indispensable role in alloimmunity in the absence of the classic CD28/CTLA-4 : B7 pathway, suggesting a synergistic/additive effect with other agents such as CTLA-4 on inhibition of unwanted immune responses. (hindawi.com)
  • Liver but not spleen pDCs suppress delayed-type hypersensitivity responses to OVA, an effect that is lost with Ebi3 −/− and B7-H1 −/− liver pDCs compared with wild-type liver pDCs. (jimmunol.org)
  • In summary, B7-H1 encompasses a potent independent predictor of prognosis for patients with RCC and an extremely promising target to facilitate immunotherapeutic responses during the management of this treatment-refractory tumor. (aacrjournals.org)
  • Further, the B7-H2-ICOS signal induces IL-10 production, which plays an important role in modulating and damping immune responses ( 14 , 17 ). (aacrjournals.org)
  • Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. (rupress.org)
  • However, after antigen uptake and exposure to stimulatory signals, DCs mature. (springer.com)
  • B7-H4 transfection vector-pGCshB7-H4 was successfully constructed. (ersjournals.com)
  • 10. The method of claim 9 , wherein the antigen is selected from the group consisting of a viral antigen and a tumor antigen. (google.com)
  • 5 . A method according to claim 4 wherein the antigen is selected from the group consisting of tumor antigens, autoimmune antigens and an antigen isolated from a pathogenic organism. (google.com)
  • 8 . A method according to claim 7 wherein the antigen is GP100 or modified gp100 having the sequence as shown in FIG. 2 (SEQ.ID.NO.:2). (google.com)
  • 10 . A method according to any one of claims 1 - 9 wherein the antigen is administered as a nucleic acid sequence encoding the antigen. (google.com)
  • Transgenic mice that express CEA as a self-antigen with a tissue distribution similar to that of humans have been developed. (aacrjournals.org)
  • No significant cross-reactivity or interference between B-Lymphocyte Activation Antigen B7-2 (LAB7-2) and analogues was observed. (biotecnika.org)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested 20 times on one plate, respectively. (biotecnika.org)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested on 3 different plates, 8 replicates in each plate. (biotecnika.org)
  • 100ug-Anti-B-Lymphocyte Activation Antigen B7-2 (LAB7-2) cat. (anigenetics.com)