Heterogeneous group of immunodeficiency syndromes characterized by hypogammaglobulinemia of most isotypes, variable B-cell defects, and the presence of recurrent bacterial infections.
An immunologic deficiency state characterized by an extremely low level of generally all classes of gamma-globulin in the blood.
A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN A.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A classification of B-lymphocytes based on structurally or functionally different populations of cells.
A dysgammaglobulinemia characterized by a deficiency of IMMUNOGLOBULIN G.
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
Polyploidy with three sets of chromosomes. Triploidy in humans are 69XXX, 69XXY, and 69XYY. It is associated with HOLOPROSENCEPHALY; ABNORMALITIES, MULTIPLE; PARTIAL HYDATIDIFORM MOLE; and MISCARRAGES.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
An island in the Gulf of St. Lawrence constituting a province of Canada in the eastern part of the country. It is very irregular in shape with many deep inlets. Its capital is Charlottetown. Discovered by the French in 1534 and originally named Ile Saint-Jean, it was renamed in 1799 in honor of Prince Edward, fourth son of George III and future father of Queen Victoria. (From Webster's New Geographical Dictionary, 1988, p981 & Room, Brewer's Dictionary of Names, 1992, p433)
An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, SARCOPHAGIDAE, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).
A component of the PUBLIC HEALTH SERVICE that provides leadership related to the delivery of health services and the requirements for and distribution of health resources, including manpower training.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Central nervous system vasculitis that is associated with SYSTEMIC LUPUS ERYTHEMATOSUS. Clinical manifestations may include DEMENTIA; SEIZURES; CRANIAL NERVE DISEASES; HEMIPARESIS; BLINDNESS; DYSPHASIA; and other neurological disorders.
Glomerulonephritis associated with autoimmune disease SYSTEMIC LUPUS ERYTHEMATOSUS. Lupus nephritis is histologically classified into 6 classes: class I - normal glomeruli, class II - pure mesangial alterations, class III - focal segmental glomerulonephritis, class IV - diffuse glomerulonephritis, class V - diffuse membranous glomerulonephritis, and class VI - advanced sclerosing glomerulonephritis (The World Health Organization classification 1982).
A classification of lymphocytes based on structurally or functionally different populations of cells.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A PREDNISOLONE derivative with similar anti-inflammatory action.
A synthetic anti-inflammatory glucocorticoid derived from CORTISONE. It is biologically inert and converted to PREDNISOLONE in the liver.
A condition characterized by the presence of abnormal quantities of CRYOGLOBULINS in the blood. Upon cold exposure, these abnormal proteins precipitate into the microvasculature leading to restricted blood flow in the exposed areas.
Antibodies found in adult RHEUMATOID ARTHRITIS patients that are directed against GAMMA-CHAIN IMMUNOGLOBULINS.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
ERYTHROCYTE size and HEMOGLOBIN content or concentration, usually derived from ERYTHROCYTE COUNT; BLOOD hemoglobin concentration; and HEMATOCRIT. The indices include the mean corpuscular volume (MCV), the mean corpuscular hemoglobin (MCH), and the mean corpuscular hemoglobin concentration (MCHC).
A plant genus of the family POACEAE. Folin is the water-soluble extract from Sasa albomarginata. Sasa kurinensis is an ingredient of Sho-ju-sen, a Japanese herbal medicine.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.

IgA production in MHC class II-deficient mice is primarily a function of B-1a cells. (1/1715)

Mice deficient in MHC class II expression (C2d mice) do not make antibody to protein antigens administered systemically, but their ability to produce IgA antibody to antigen administered at mucosal sites has not been described. We investigated IgA production by C2d mice and their IgA antibody response to antigen given orally. Young C2d mice had normal amounts of serum IgA, intestinal-secreted IgA and normal numbers of intestinal IgA plasma cells, compared to control C57BL/6 mice. IgA production by C2d mice increased with age. Following oral immunization with cholera toxin, C57BL/6 mice responded with IgA and IgG antibody, and had increased numbers of IgA plasma cells, but C2d mice gave no response. The Peyer's patch and mesenteric lymph node tissues of C2d mice contained very few CD4-expressing T cells. Thus, C2d mice have no typical mucosal CD4 Th cells and cannot respond to a strong oral immunogen, yet they still produced and secreted IgA. We hypothesized that B-1 lymphocytes could provide a source of IgA independent of antigen-specific T cell help. Young C2d mice had normal numbers of peritoneal B-1a cells and their frequency increased with age. To test the role of these B-1a cells, we bred C2d mice to obtain mice that had no MHC class II expression and expressed the Xid gene that confers deficiency in B-1a cells. These double-deficient mice had 10-fold less serum and secreted IgA than all other F2 littermates. We conclude that B-1a cells are essential for the majority of IgA production in C2d mice. Thus, the C2d mouse may provide a useful tool for analysis of the role of intestinal IgA provided by B-1a cells.  (+info)

An alternatively spliced form of CD79b gene may account for altered B-cell receptor expression in B-chronic lymphocytic leukemia. (2/1715)

Several functional anomalies of B-chronic lymphocytic leukemia (B-CLL) cells may be explained by abnormalities of the B-cell receptor (BCR), a multimeric complex formed by the sIg homodimer and the noncovalently bound heterodimer Igalpha/Igbeta (CD79a/CD79b). Because the expression of the extracellular Ig-like domain of CD79b has been reported to be absent in the cells of most CLL cases, we have investigated the molecular mechanisms that may account for this defect. Peripheral blood lymphocytes (PBL) from 50 patients and two cell lines (MEC1, MEC2) obtained from the PBL of one of them were studied. MEC1, MEC2, and 75% of CLL cases did not express detectable levels of the extracellular Ig-like domain of CD79b, which was nevertheless present in greater than 80% CD19(+) cells from normal donors. In healthy subjects the expression of CD79b was equally distributed in CD5(+) and CD5(-) B-cell subsets. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of CD79b RNA from all patients and from MEC1 and MEC2 cell lines consistently yielded two fragments of different size (709 bp and 397 bp). The 709-bp band corresponds to CD79b entire transcript; the 397-bp band corresponds to an alternatively spliced form lacking exon 3 that encodes the extracellular Ig-like domain. Both fragments were also visible in normal PBL. The expression of the 397-bp fragment was increased in normal activated B cells, while no difference was seen between CD5(+) and CD5(-) B cells. To obtain a more accurate estimate of the relative proportions of the two spliced forms, a radioactive PCR was performed in 13 normal and 22 B-CLL samples and the results analyzed using a digital imager. The mean value of the CD79b to the CD79b internally deleted ratio was 0.64 +/- 0.20 SD in normal donors and 0.44 +/- 0.27 SD in B-CLL (P =.01). Direct sequencing of 397-bp RT-PCR products and of genomic DNA corresponding to exon 3 from MEC1, MEC2, their parental cells, and five fresh B-CLL samples did not show any causal mutation. Single-strand conformation polymorphism analysis of exon 3 performed in 18 additional B-CLL cases showed a single abnormal shift corresponding to a TGT --> TGC polymorphic change at amino acid 122. We propose a role for the alternative splicing of CD79b gene in causing the reduced expression of BCR on the surface of B-CLL cells. As normal B cells also present this variant, the mechanism of CD79b posttranscriptional regulation might reflect the activation stage of the normal B cell from which B-CLL derives.  (+info)

Anti-phospholipid antibodies and CD5+ B cells in HIV infection. (3/1715)

This cross-sectional study evaluates the correlation between anti-phospholipid antibodies and CD5+ B cells in 110 patients infected with HIV-1. There were 89.1% of the patients who had IgG antibodies against cardiolipin and phosphatidylserine. The prevalence of IgM and IgA antibodies was < 22%. AIDS was associated with lower frequencies of IgM antibodies against cardiolipin (P = 0.05) and IgG-antibodies against cardiolipin and phosphatidylserine (P = 0.011). Drug users had higher IgM antibodies against phospholipids than patients from other risk groups (P = 0.02). A history of thromboembolic events was not accompanied by higher levels of anti-phospholipid antibodies (P > 0.2). No correlation between anti-phospholipid antibodies and CD5+ B cells was detected. Percentage part of CD5+ B lymphocytes was elevated in all patients and absolute CD4+ T lymphocyte counts and HIV p24 antigen were inversely correlated. In advanced disease a significant reduction of anti-phospholipid antibodies was contrasted with persistent elevation of CD5+ B lymphocytes. These observations may reflect immunological dysfunction involving apoptosis and endothelial damage rather than polyclonal B cell hyperstimulation. A possible explanation would be that in HIV infection an increased rate of spontaneous apoptosis in peripheral blood lymphocytes is accompanied by functional and structural changes of mitochondria. Therefore, structurally altered mitochondrial phospholipids could serve as antigen to induce specific humoral immune responses.  (+info)

Cutting edge: recruitment of the CD19/CD21 coreceptor to B cell antigen receptor is required for antigen-mediated expression of Bcl-2 by resting and cycling hen egg lysozyme transgenic B cells. (4/1715)

Recruitment of the CD19/CD21 coreceptor is thought to lower the threshold for effective signaling through the B cell Ag receptor. We provide evidence supporting a second role for coreceptor recruitment, and that is to enhance the survival/proliferative potential of the responding B cells. We show that B cell Ag receptor signaling in the absence of coreceptor recruitment induces cellular accumulation of the anti-apoptotic protein Bcl-xL, whereas CD19-mediated signals are required for Bcl-2 accumulation. The expression of both anti-apoptotic proteins correlates with the enhanced responsiveness of both resting and cycling B cells to growth-promoting signals delivered through CD40. These results provide further evidence for the necessity of coreceptor recruitment during Ag-dependent B cell activation and indicate that Ags derived from inflammatory sites function as better thymus-dependent Ags than their counterparts not coated with complement fragments.  (+info)

Antigen receptor engagement selectively induces macrophage inflammatory protein-1 alpha (MIP-1 alpha) and MIP-1 beta chemokine production in human B cells. (5/1715)

We show herein that B cell Ag receptor (BCR) triggering, but not stimulation by CD40 mAb and/or IL-4, rapidly induced the coordinated expression of two closely related T cell chemoattractants, macrophage inflammatory protein-1 beta (MIP-1 beta) and MIP-1 alpha, by human B cells. Naive, memory, and germinal center B cells all produced MIP-1 alpha/beta in response to BCR triggering. In contrast to MIP-1 alpha/beta, IL-8, which is spontaneously produced by germinal center B cells but not by naive and memory B cells, was not regulated by BCR triggering. Culturing follicular dendritic cell-like HK cells with activated B cells did not regulate MIP-1 alpha/beta production, but it did induce production of IL-8 by HK cells. Microchemotaxis assays showed that CD4+CD45RO+ T cells of the effector/helper phenotype actively migrated along a chemotactic gradient formed by BCR-stimulated B cells. This effect was partially blocked by anti-MIP-1 beta and anti-CC chemokine receptor 5 Ab, but not by anti-MIP-1 alpha Ab suggesting that MIP-1 beta plays a major role in this chemoattraction. Since maturation of the B cell response to a peptide Ag is mostly dependent on the availability of T cell help, the ability of Ag-stimulated B cells to recruit T cells via MIP-1 alpha/beta, may represent one possible mechanism enabling cognate interactions between rare in vivo Ag-specific T and B cells.  (+info)

Age-dependent altered proportions in subpopulations of tonsillar lymphocytes. (6/1715)

Age-related changes in functional subsets of lymphocytes may influence the potential to build up immune responses. In particular, the capacity of tonsillar lymphocytes to counter infections may be altered during ageing. In order to address this question we investigated the proportional distribution of several subsets of tonsillar T and B cells with regard to ageing. Tonsils were derived from 119 patients between 2 and 65 years of age. Lymphocyte subsets were monitored by three-colour fluorescence of relevant CD markers in flow cytometry. As a general tendency the percentage of CD3+ T cells steadily increased whereas that of CD19+ B cells decreased at the same time. No significant differences were observed between lymphocytes of patients with and without inflammatory history of the tonsils. The percentage of CD8+ T cells declined whereas that of CD4+ T cells increased during the same time span. CD45RA+ T cells increased during the first two decades of life and gradually decreased thereafter. In contrast, CD45RO+ T cells showed an opposite trend. No differences were seen in the population of CD3-/CD56+ natural killer (NK) cells. The mature B cell marker CD40 showed no significant changes during ageing. However, CD38+ B cells, representing B cells of late maturation stages, dramatically declined up to the age of 65. In a similar manner the CD5+ subpopulation of B cells decreased during ageing. Substantial changes in major tonsillar T and B cell populations as shown in this study may have an impact on the ageing process of the immune system.  (+info)

The evolutionarily conserved sequence upstream of the human Ig heavy chain S gamma 3 region is an inducible promoter: synergistic activation by CD40 ligand and IL-4 via cooperative NF-kappa B and STAT-6 binding sites. (7/1715)

Germline C gamma gene transcription is a crucial event in the process that leads to switch DNA recombination to IgG, but its regulation in the human is poorly understood. We took advantage of our monoclonal model of germinal center B cell differentiation, IgM+ IgD+ CL-01 cells, to define the role of the I gamma 3 evolutionarily conserved sequence (ECS) in the germline transcriptional activation of the human C gamma 3 gene. The I gamma 3 ECS lies upstream of the major I gamma 3 transcription initiation site and displays more than 90% identity with the corresponding human I gamma 1, I gamma 2, and I gamma 4 regions. Reporter luciferase gene vectors containing the human gamma 3 ECS were used to transfect CL-01 cells, which have been shown to undergo Smu-->S gamma 3 DNA recombination, upon engagement of CD40 by CD40 ligand (CD40L) and exposure to IL-4. In these transfected CL-01 cells, CD40:CD40L engagement and exposure to IL-4 synergistically induced gamma 3 ECS-dependent luciferase reporter gene activation. Targeted mutational analysis demonstrated that a tandem NF-kappa B/Rel binding motif is critical for the gamma 3 ECS responsiveness to both CD40L and IL-4, while a STAT-6-binding site is additionally required for IL-4 inducibility. Electrophoretic mobility shift assays showed that p50/p65/c-Rel and STAT-6 are effectively induced by CD40L and IL-4, respectively, and bind to specific DNA motifs within the ECS. These partially overlapping CD40L and IL-4 responsive elements are functionally cooperative as the disruption of one of them prevents synergistic promoter activation. Thus, the gamma 3 ECS is an inducible promoter containing cis elements that critically mediate CD40L and IL-4-triggered transcriptional activation of the human C gamma 3 gene.  (+info)

Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. (8/1715)

Our previous studies indicated that stromal cell-derived syndecan-4 might mediate some form of communication with pre-B cells in bone marrow. We now report additional aspects of this recognition and show that syndecan-4 is also present on pre-B cells. Indeed, the molecule is acquired at an early stage of differentiation and retained until mature B cells undergo Ig isotype switching. mAbs developed to two portions of the syndecan-4 protein core were used to probe possible functions on B lineage lymphocytes. Syndecan-4 ligation had no obvious influence on B lymphocyte formation or activation, but this treatment caused a dramatic morphological change in appropriately stimulated leukocytes. Extended filopodia appeared on transfected Ba/F3 or FDCP-1 cells, as well as activated B cell blasts that were placed on syndecan-4 Ab-coated surfaces. The dendritic processes contained polymerized actin as well as pp52(LSP1), a prominent F-actin binding protein in lymphocytes. The cytoplasmic domain of syndecan-4 was not required for this response. Shape changes of this type could facilitate interactions between B lymphocytes and other components of the immune system. Not only is syndecan-4 a useful marker for discriminating normal B lineage lymphocyte subsets, but our results suggest new ways for the syndecans to participate in immune responses.  (+info)

TY - JOUR. T1 - Aged mice exhibit distinct B cell precursor phenotypes differing in activation, proliferation and apoptosis. AU - Van der Put, Elaine. AU - Sherwood, Erin M.. AU - Blomberg, Bonnie B. AU - Riley, Richard L. PY - 2003/10/1. Y1 - 2003/10/1. N2 - Senescence in murine models is associated with a reduction, albeit heterogeneous, in bone marrow pre-B cells. We have categorized aged BALB/c mice into two phenotypes based on their patterns of pre-B/pro-B cell loss. Each phenotype is characterized by distinct responses to the growth cytokine IL-7 and capacity for survival in vitro. A moderate loss of late-stage pre-B cells (25-80%) coincided with decline in proliferation to rmIL-7. This was also associated with a decrease in the frequency of pro-B cells which increased phosphotyrosine content upon IL-7 stimulation, an indicator of early activation events. A severe loss of pre-B cells (,80%) resulted in a reduced pro-B cell pool which retained normal activation and proliferative ...
Our views regarding the origins and functions of splenic marginal zone B cells have changed considerably over the past few years. Perspectives regarding the development and function of these cells vary considerably between investigators studying human and rodent immunology. Marginal zone B cells are …
The development of an effective Ab response to TD Ag requires collaboration between T and B cells. Several lines of evidence support the idea that the ability of B cells to induce both naive T cell priming and tolerance depends on their resting vs activated state (28, 29, 30, 39, 40, 41, 42, 43, 44). In agreement with previous results, we found that both resting MZ and FO B cells were unable to induce T cell responses in vivo and in vitro. However, within a few hours of in vivo Ag priming, MZ B cells were far superior to FO B cells in the activation of naive CD4+ T cells in vitro. MD4 B cell populations have been shown to be a little different in the phenotype of their subsets, in that IgM on FO B cells tended to persist at higher levels than on FO B in normal mice. However, we sorted FO and MZ B cell subsets based on their expression of CD23 and CD21. In this respect the MZ B cells are clearly distinguishable from their FO counterparts. Despite this anomaly in MD4 transgenic mice MZ and FO B ...
The mechanisms that establish immune tolerance in immature and mature B cells appear to be distinct. Membrane-bound autoantigen is thought to induce developmental arrest and receptor editing in immature B cells, whereas mature B cells have shortened lifespans when exposed to the same stimulus. In this study, we used Emu-bcl-2-22 transgenic (Tg) mice to test the prediction that enforced expression of the Bcl-2 apoptotic inhibitor in B cells would rescue mature, but not immature, B cells from tolerance induction. To monitor tolerance to the natural membrane autoantigen H-2Kb, we bred 3-83mudelta (anti-Kk,b) Ig Tg mice to H-2(b) mice or to mice expressing transgene-driven Kb in the periphery. In 3-83mudelta/bcl-2 Tg mice, deletion of autoreactive B cells induced by peripheral Kb antigen expression in the liver (MT-Kb Tg) or epithelia (KerIV-Kb Tg), was partly or completely inhibited, respectively. Furthermore, Bcl-2 protected peritoneal B-2 B cells from deletion mediated by acute antigen exposure, ...
Quantitative variation in the expression of MHC-encoded class II (Ia) glycoproteins has been associated with stages of lymphocyte development and a number
Replacement nurses and technicians will arrive over the weekend for orientation sessions in preparation for a possible strike at Lawrence + Memorial
The uniqueness of each BCR and width of the B cell repertoire are due to the DNA rearrangements at HC and LC loci. B cells leave the bone marrow at the transitional stage carrying a wide array of germline-encoded and randomly assembled Ig genes. Transitional B cells migrate with the blood to the spleen. They are not fully functional in the immune responses: they are not able to respond to inflammatory chemokines, and engagement of the BCR leads to their apoptotic cell death. These are safety measures against autoreactivity preventing self-reactive transitional B cells from further development and migration to sites rich in cytokines and stimulatory cells. In contrast to the mortal effect of BCR cross-linking, engagement of TLR9 leads to differentiation of transitional B cells. In response to CpG, a fraction of the transitional B cells (26%) proliferates and acquires the phenotype of memory B cells or directly matures into plasma cells. The remaining cells start to express the markers of ...
In the past decade, the suppressive effects, mainly through the secretion of IL-10, of regulatory B cells on inflammatory responses have been reported in a variety of immune disorders (33-36). Additionally, immune regulation through the interaction of immune cells with the intrinsic phenotype of regulatory B cells (e.g., CD1dhiCD5+, T2-MZP, Tim-1+, and CD9+) were demonstrated in various diseases, and it plays a critical role in autoimmune diseases (37). In recent studies, functional studies in cancer diseases are emerging (38-40). In particular, the change of the distribution of regulatory B cells in cancer tissue is considered to one of important indicators (8-10). Emerging evidence suggests that regulatory B cells suppress effector immune cells including IFN-γ-producing cytotoxicity cells in various cancer diseases through the secretion of IL-10 (11). Although regulatory B cells have to play the suppressive role on the effector function of T cells in autoimmune diseases to cure diseases (41), ...
Marginal zone (MZ) B cells are thought to be responsible for the first wave of Abs against bacterial Ags. In this study, we assessed the in vivo response of MZ B cells in mice immunized with viral particles derived from the RNA phage Qbeta. We found that both follicular (FO) and MZ B cells responded to immunization with viral particles. MZ B cells responded with slightly faster kinetics, but numerically, FO B cells dominated the response. B1 B cells responded similarly to MZ B cells. Both MZ and FO B cells underwent isotype switching, with MZ B cells again exhibiting faster kinetics. In fact, almost all Qbeta-specific MZ B cells expressed surface IgG by day 5. Histological analysis demonstrated that a population of activated B cells remain associated with the MZ, probably due to the elevated integrin levels expressed by these cells. Thus, both MZ and FO B cells respond with rapid proliferation to viral infection and both populations undergo isotype switching, but MZ B cells remain in the MZ and may be
We originally cloned and identified murine Zizimin2 (Ziz2, Dock11) as a guanine nucleotide exchange factor (GEF) for Cdc42 and demonstrated that it activated the formation of filopodia. Since its expression pattern is restricted in immune tissues and Rho GTPases such as Cdc42 function in B cell deve …
New insights into human B cell biology. B cells are highly important white blood cells known as lymphocytes and are part of the adaptive immune system. B cells have a specialised receptor on their cell surface (B cell receptor, BCR) which recognises specific proteins. Upon activation, B cells produce antibodies which bind antigens like e.g. molecules from pathogens or vaccines. The drawback of a vast range of different B cell receptors is the potential that some of the receptors recognise self-antigens which can then result in auto-immune disorders. The bone marrow continuously releases immature B cells into the blood stream. A high proportion of these so-called transitional B cells are able to recognise self-antigens via their BCR. It has been unknown where in the body these auto-reactive cells are checked and removed from the circulation. A recent publication by Anna Vossenkämper and Jo Spencer (Kings College) tracked the fate of human transitional B cells and identified that these cells ...
Notch2 interaction with its ligand, Dll1, is required in the mouse to drive MZP into the MZB cell lineage (Saito et al., 2003; Hozumi et al., 2004). Preliminary data based on humanized mouse models have also proposed a Notch2 dependence for the differentiation of IgM+IgD+CD27+ B cells (Scheeren et al., 2008). Accordingly, we searched for an MZP in the spleen from young children, taking as diagnostic criteria its capacity to acquire an MZ phenotype when cultured in presence of OP9 cells expressing human DLL1, a differentiation which, moreover, should be specifically inhibited in presence of anti-NOTCH2 blocking antibodies. This precursor subset was identified using the recently described MEM55 antibody, which marks a glycosylated variant of the CD45RB molecule, harbored by CD27+ B cells and an immature B cell subset (Koethe et al., 2011). Surprisingly, these MZPs were further characterized as expressing the ABCB1 transporter reported so far as the unique hallmark of naive B cells (Wirths and ...
Vol. 203, No. 11, October 30, 2006. Pages 2425-2431.. Please note that an error appeared in the original online early release version of this article. A portion of text from the second paragraph in the Results and Discussion section was erroneously deleted. The current html, print, and pdf versions appear correctly. For reference, the corrected paragraph appears below in its entirety.. We next assessed if these long-lived t(14;18)-bearing B cells in HI already transited through the GCs. Circulating naive and memory B cells can be distinguished on the basis of the CD27 cell surface marker and sIg isotypes (11-13). Fresh PBMCs from 10 healthy donors were collected, and IgD+/CD27− naive, IgD+/CD27+ memory, and IgD−/CD27+ switched memory B cell subsets isolated by cell sorting (Fig. 2 A). The occurrence of t(14;18) was then assessed in total PBMCs and in each fraction by the short-range BCL2/JH PCR assay (Fig. 2 B and Table II). As a first approach, we pooled data from the two CD27+ memory ...
The production of antibody to a thymus-dependent Ag requires cooperation between the B cell and an Ag-specific Th cell. MHC restriction of this interaction implies that the Th cell recognizes Ag on the B cell surface in the context of MHC molecules and that the Ag-specific B cell gets help by acting as an APC for the Th cell. However, a number of studies have suggested that normal resting B cells are ineffective as APC, implying that the B cell must leave the resting state before it can interact specifically with a Th cell. Other studies, including our own with rabbit globulin-specific mouse T cell lines and hybridomas, show that certain T cell lines can be efficiently stimulated by normal resting B cells. One possible explanation for the above contradiction is that our B cells have become activated before presentation. Here we show that presentation by size-selected small B cells is not the result of nonspecific activation signals generated by the T cells or components of the medium. Also, although LPS
As with people, identity is vital to cells. When a cell loses its identity, it can stop working properly and a range of illnesses can result. The immune system, which protects our bodies from disease, includes cells with many different identities. When these cells lose their identity it can cause certain cancers or increase the risk of infections.. Complex networks of signals and genes create and maintain the identity of different cells. New research from the Babraham Institute, Cambridge and the University of Birmingham has revealed how a protein called ZFP36L1 helps cells known as marginal zone B cells (MZ B cells) to maintain their identity.. For cells, identity describes how they are adapted to have a specialised function. Blood cells are specialised for transportation, nerve cells for communication and the immune system fights infections. Each cell becomes specialised to do its job as a result of unique combinations of genetic instructions, which influence how the cell works.. MZ B cells ...
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Normal peripheral B cells can be activated by and cultured in interleukin 4 and anti-CD40 monoclonal antibodies. Is it possible to perform either stable or transient transfection assays in these cells ? Thanks for references if any. I am particularly interested in studying the expression of various p53 mutants ...
Martin, VG, Wu, Y-CB, Townsend, CL, Lu, GH, OHare, JS, Mozeika, A, Coolen, AC, Kipling, D, Fraternali, F and Dunn-Walters, Deborah (2016) Transitional B cells in early human B cell development - time to revisit the paradigm? ...
This lesson will focus in on the generalities of B-cells, such as their place of generation, maturation, and training, as well as some specific...
B Cell小鼠单克隆抗体[CA2.1D6](ab34125)可与马, 猫样本反应并经IP, IHC, Flow Cyt实验严格验证,被3篇文献引用。所有产品均提供质保服务,中国75%以上现货。
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Results High expression levels of TLR7 in SLE patients positively correlated with IFN signature and disease activity, but not with BAFF titers. SLE patients with high levels of TLR7 (TLR7hi group) showed an expansion of CD19+CD38highCD24highCD10+ TR B cells. Overall, frequencies of TR B cells positively correlated with the levels of TLR7, but not TLR9. SLE patients, carrying a risk G allele, had increased TLR7 expression and TR cell frequencies, compared to non-risk allele carriers. TLR7hi SLE patients showed increased autoantibody titers and skewing towards Sm/RNP antigens. Upon IFNα priming, TR B cells up-regulated TLR7 and differentiated into plasmablasts in response to TLR7-ligand stimulation. ...
Carole Goutsmedt, Laëtitia Le Pottier, Jacques-Olivier Pers. Identification of an antigen-specific regulatory B cell subset in humans.. 35th European Workshop for Rheumatology Research, Mar 2015, Budapest, Hungary. 74 (Supplément 1 A1.27), pp.A11, 2015, Annals of the Rheumatic Diseases. 〈hal-01128705〉 ...
B cells are known to play an important role in auto-immune diseases by activating T cells, secreting inflammatory cytokines and autoreactive antibodies. However, a sub-type of B cells named regulatory B cells or Bregs has recently shown capacities to prevent or cure arthritis in mouse models. Bregs have also been identified in humans. Main objective: To study Bregs abnormalities in patients with rheumatoid arthritis (RA) at different stages of the disease compared to subjects with mechanical pathologies.Secondary objectives:- To evaluate the specificity of any abnormalities identified in RA by studying Bregs in patients with other autoimmune or other inflammatory joint diseases.- To evaluate the effect of biological and synthetic treatments on Bregs in patients with RA. - To assess whether the rate of Bregs before treatment is predictive of response to biological and synthetic treatments ...
Original citation: J. Clin. Invest.112:286-297 (2003). doi:10.1172/JCI18025.. Citation for this corrigendum: J. Clin. Invest.113:1069 (2004). doi:10.1172/JCI18025E1.. The legends for Figures 6 and 7 contained inaccuracies, and the correct versions appear below. The conclusions of the article are unaffected.. Figure 6 BAFF increases the generation of ISC from activated memory B cells. (a and b) Memory B cells were preactivated with CD40L and IL-2/IL-10 for 4 days and then recultured with (a) media (black bars), or (b) IL-2/IL-10 alone (black bars) or in the presence of CD40L (white bars) or BAFF (gray bars). Each value represents the mean Ig secretion ± SEM of five (a) or seven (b) experiments using cells from different donors. *P , 0.05; **P , 0.01. (c) Secondary B cell cultures were performed in the absence (black bars) or presence (white bars) of soluble TACI-Ig (20 μg/ml). The values represent the mean IgA ± SD of duplicate samples. (d) Memory B cells were preactivated with ...
TY - JOUR. T1 - The CD40 ligand expressed by human B cells costimulates B cell responses. AU - Grammer, A. C.. AU - Bergman, M. C.. AU - Miura, Y.. AU - Fujita, K.. AU - Davis, L. S.. AU - Lipsky, P. E.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - The possibility that activated B cells might express a ligand for CD40 that was of functional importance for B cell responses was examined by using highly purified human peripheral blood B cells, as well as a variety of B lymphoblastoid cell lines and hybridomas. Following stimulation with the combination of a calcium ionophore and a phorbol ester, human B cells bound a soluble fusion protein containing the extracellular portion of CD40 and the Fc region of lgG1 (CD40.lg). A variety of B cell lines and hybridomas also bound CD40.1g, either constitutively or after activation. In addition, CD40.Ig specifically immunoprecipitated a 33-kDa glycoprotein from surface 125I-labeled activated B cells. The nucleotide sequence of the coding region of the CD40 ligand mRNA ...
Results Exogenously added IL-7 did not activate B cells directly, in line with the absence of surface IL-7R. However, in the presence of T cells, IL-7 activated both T and B cells (Ki67+ CD4 cells from 1.1±0.2% to 14.4±3.7%, p,0.01 and Ki67+ B cells from 1.9±0.3% to 4.1±0.9%, p,0.05). TLR7A induced B cell activation, as measured by increased proliferation (%Ki67 from 1.2±0.2% to 9.3±1.4%) and up regulation of activation markers on B cells, which was facilitated in the presence of monocytes. TLR7-induced B cell activation in T/B or T/B/monocyte co-cultures was not associated with T cell activation. IL-7 added to TLR7A synergistically increased both B cell (TLR7A vs. IL-7/TLR7A; 9.3±1.4% vs. 33.4±7.3%) and T cell proliferation (IL-7 vs. IL-7/TLR7A; 0.8±0.1% vs. 29.2±5.2%), which for B cells again was further increased by monocytes (TLR7A vs. IL-7/TLR7A; 30.2±8.9% vs. 63.0±8.0%). Similar results were observed for activation marker expression on B cells (CD19, HLA-DR CD25) and on T cells ...
B1 cells are a sub-class of B cell lymphocytes that are involved in the humoral immune response. They are not part of the adaptive immune system, as they have no memory, but otherwise, B1 cells perform many of the same roles as other B cells: making antibodies against antigens and acting as antigen presenting cells. Notably, most B1 cells do not develop into memory B cells. B1b cells have been shown to be capable of memory responses. See B1b lymphocytes confer T cell-independent long-lasting immunity. B1 cells are first produced in the fetus and most B1 cells undergo self-renewal in the periphery, unlike conventional B cells (B2 cells) that are produced after birth and replaced in the bone marrow. In January 2011, human B1 cells were found to have marker profile of CD20+CD27+CD43+CD70- and could either be CD5+ or CD5-, which has been debated since. CD5-CD72 is thought to mediate B cell-B cell interaction. B-1 B cells, in the mouse, can be further subdivided into B-1a (CD5+) and B-1b (CD5−) ...
To determine the phenotype of isolated B cells and differentiation on day 14 of culture, a gating strategy was developed for analysis of cells by flow cytometry. After debris and doublets were excluded, viable CD19+ cells were selected and analyzed for the proportions of naive and memory cells according to their CD27 and IgD expression. Viable plasma cells were determined as CD138+CD38+ cells. B cells cultured with the kit seemed to resemble untreated B cells in their subset distribution. The amounts of naive B cells decreased on day 14. This is in line with reports describing that naive B cells have a shorter survival rate. In contrast, the amounts of non-switched memory B cells increased in both the unstimulated culture and the culture expanded by the B Cell Expansion Kit indicating a greater survival capacity of this subset.. ...
This study demonstrates that patients with active, untreated IgG4-RD have significant elevations in their circulating plasmablast counts regardless of their serum IgG4 concentrations, and that patients with multi-organ IgG4-RD have higher absolute plasmablast counts than those with involvement of only one or two organs. In addition, plasmablast levels appear to be superior to serum IgG4 concentrations as a biomarker for IgG4-RD. Plasmablast counts decline swiftly following peripheral B cell depletion, and this decline is accompanied by corresponding decreases in disease activity as measured by the IgG4-RD RI. IgG4-RD thus represents an unusual example of an immune-mediated condition in which measurement of a single cell type may play a central role in diagnosing, monitoring and managing the disease.. The identification of circulating plasmablast expansion in IgG4-RD has substantial implications for clinical care. The diagnosis of IgG4-RD remains dependent upon biopsy and is therefore subject to ...
B cell subsets have been found to exhibit a negative regulatory function, like Tregs. The present study investigates the effects of CD5+CD19+ interleukin (IL)-10 (B10) on the occurrence and development of oesophageal carcinoma by analysing B10 levels in the peripheral blood of patients with...
Clone REA450 recognizes the rat CD45R antigen, an molecule which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Expression levels of CD45R are different among distinct B cell subpopulations and appears to be an indicator of maturational stages.Additional information: Clone REA450 displays negligible binding to Fc receptors. - Italia
B cells are crucial players in the human immune system which secrete antibodies that can eliminate bacterial antigens. An agent-based model is implemented to study the behaviour between B cell-antigen interactions during a bacterial infection. We have determined that infection in the system occurs when 69% of antigens are eliminated. Cellular lifetime of B cells plays a role in clearing the antigens, exhibiting three distinct phases - the lag phase, exponential growth phase, and the stationary phase. A Gompertz curve fit demonstrates that the B cell population curve mimics a biologically realistic phenomena. Studying the population dynamics of the system shows that B cell population peaks at around 60%-70% of antigens are eliminated, further supporting the determined point of infection.. ...
Xu S, Huo J, Huang Y, Aw M, Chen S, Mak S, Yip LY, Ho YS, Ng SW, Tan AH, Lee A, Ou X, Lam KP. von Hippel-Lindau Protein Maintains Metabolic Balance to Regulate the Survival of Naive B Lymphocytes. iScience. 2019 Jul 26;17:379-392 ...
TIM-1 defines a human regulatory B cell (Bregs) population that is altered in frequency and function in systemic sclerosis (SSc) patients. TIM-1 is a unique marker for the identification of a human IL-10+ Breg subpopulation which is partially superimposed with transitional B cells. Alterations in TIM-1+ B cells could contribute to the development of autoimmune diseases such as SSc. PubMed, Arthritis Res Ther, 2017 Jan 19;19(1):8. (Also see B Cells and T Cells) This item was posted in the ISN Newsroom. Please check the newsroom daily for updates on scleroderma and other related articles ...
Cd19 B Cell Activation, Bcr, Tcr, B Cell Co-receptor - B Cell Activation Cd21 Clipart is high quality 1023*612 transparent png stocked by PikPng. Download it free and share it with more people.
Regulation of inhibitory IgSF receptors in memory B cells by IL-4. Naive and memory B cells were purified from peripheral blood and cultured either in medium al
The process in which immature B cells from the bone marrow acquire the specialized features of T1 stage B cells in the spleen. T1 stage B cells do not express either CD23 or CD21.
Stimulation by antigen through the B cell receptor (BCR) followed by cognate T cell help drives proliferation and differentiation of antigen-specific naı̈ve B lymphocytes into memory B cells and plasma cells (1, 2). Memory B cells carrying somatically mutated immunoglobulin (Ig) genes survive in secondary lymphoid organs in the absence of antigen (3) and mediate secondary immune responses upon rechallenge. In contrast, plasma cells are terminally differentiated, nondividing cells that home to spleen and bone marrow and are the main source of antibody, which they secrete at a high rate. Mouse plasma cells can be long-lived and are able to sustain antibody production for several months in the absence of memory B cells or antigen (4, 5). However, it is less likely that long-lived plasma cells produced during an immune response will maintain a constant supply of specific antibody over a human life-span, because even long-lived plasma cells would eventually need to be replenished over a human ...
Secretory IgA (S-IgA) is a hallmark antibody principally produced at mucosal sites and plays an important role in the creation of immunological surveillance and homeostasis at mucosa. In addition to the IgA induction through gut-associated lymphoid tissues (e.g., Peyers patch), peritoneal B cells have been considered to be another source of S-IgA, especially specific for the T-independent antigen. Here we show that the trafficking of peritoneal B cells is principally regulated by sphingosine 1-phosphate (S1P). Peritoneal B cells expressed high levels of the type 1 S1P receptor. Thus, disruption of S1P-mediated signaling caused a rapid disappearance of peritoneal B cells. These changes did not affect natural plasma antibody production or phosphorylcholine (PC)-specific antibody production in serum after peritoneal immunization with heat-killed streptococcal pneumoniae. However, it dramatically reduced peritoneal B cell-derived natural intestinal S-IgA production without affecting the expression ...
Antibody affinity maturation occurs in germinal centers (GCs) through iterative rounds of somatic hypermutation and selection. Selection involves B cells competing for T cell help based on the amount of antigen they capture and present on their MHC class II (MHCII) proteins. How GC B cells are able to rapidly and repeatedly transition between mutating their B cell receptor genes and then being selected shortly after is not known. We report that MHCII surface levels and degradation are dynamically regulated in GC B cells. Through ectopic expression of a photoconvertible MHCII-mKikGR chimeric gene, we found that individual GC B cells differed in the rates of MHCII protein turnover. Fluctuations in surface MHCII levels were dependent on ubiquitination and the E3 ligase March1. Increases in March1 expression in centroblasts correlated with decreases in surface MHCII levels, whereas CD83 expression in centrocytes helped to stabilize MHCII at that stage. Defects in MHCII ubiquitination caused GC B cells to
Immunity to individual group A rotavirus (RV), a major cause of viral gastroenteritis in infants, involves B lymphocytes that provide RV-specific antibodies. VP7 interactions with B cells were mediated by surface immunoglobulins and probably by their Fab portions. VP7-reactive B lymphocytes were mainly detected from RV-experienced patients and almost exclusively in the CD27-positive memory cell fraction. Conversely, VP6-reactive B lymphocytes were detected at comparable and high frequencies in adult, infant, and neonate samples. In adult samples, VP6 reacted with about 2% of the CD27-unfavorable (CD27neg) naive B cells. These results demonstrated that this VP6 RV protein interacted with a large fraction of naive B lymphocytes from both adults and neonates. We propose that naive B cell-VP6 conversation might impact the power and quality from the obtained immune system response and really should be looked at for elaborating RV vaccine strategies. Individual group A rotavirus (RV) is regarded as a ...
A naive (or inexperienced) B cell is one which belongs to a clone which has never encountered the epitope to which it is specific. In contrast, a memory B cell is one which derives from an activated naive or memory B cell. The activation of a naive or a memory B cell is followed by a manifold proliferation of that particular B cell, most of the progeny of which terminally differentiate into plasma B cells;[note 8] the rest survive as memory B cells. So, when the naive cells belonging to a particular clone encounter their specific antigen to give rise to the plasma cells, and also leave a few memory cells, this is known as the primary immune response. In the course of proliferation of this clone, the B cell receptor genes can undergo frequent (one in every two cell divisions)[8] mutations in the genes coding for paratopes of antibodies. These frequent mutations are termed somatic hypermutation. Each such mutation alters the epitope-binding ability of the paratope slightly, creating new clones of ...
We show here that approximately 25% of the mature peripheral B cells can survive in the mouse for at least 2 months without Syk, in a manner that requires BAFF‐R and CD19 signaling. In contrast, deletion of the Syk gene in early B cells results in the appearance of a small number of immature IgM+ B cells, which, however, fail to give rise to any mature B cells in the periphery (Cheng et al, 1995). Thus, pre‐B and mature B cells have different requirements for Syk. Indeed, the pre‐BCR is an autonomously signaling receptor that continuously engages Syk, whereas the BCR forms an autoinhibited oligomer on mature B cells that is not in contact with Syk. This notion is supported by a proximity ligation analysis showing that Syk is localized near the BCR only after BCR activation (Infantino et al, 2010; Klasener et al, 2014).. The presence of large amounts of Syk‐negative mature B cells in the induced mb1‐CreERT2;Sykfl/fl mice allowed us to analyze the in vivo role of this kinase in the ...
We show here that approximately 25% of the mature peripheral B cells can survive in the mouse for at least 2 months without Syk, in a manner that requires BAFF‐R and CD19 signaling. In contrast, deletion of the Syk gene in early B cells results in the appearance of a small number of immature IgM+ B cells, which, however, fail to give rise to any mature B cells in the periphery (Cheng et al, 1995). Thus, pre‐B and mature B cells have different requirements for Syk. Indeed, the pre‐BCR is an autonomously signaling receptor that continuously engages Syk, whereas the BCR forms an autoinhibited oligomer on mature B cells that is not in contact with Syk. This notion is supported by a proximity ligation analysis showing that Syk is localized near the BCR only after BCR activation (Infantino et al, 2010; Klasener et al, 2014).. The presence of large amounts of Syk‐negative mature B cells in the induced mb1‐CreERT2;Sykfl/fl mice allowed us to analyze the in vivo role of this kinase in the ...
Free online memory games. metro match is the classic memory game with a twist. Aj and ep are extremes on the spectrum of human memory. and their cases say more than any brain scan about the extent to which our memories make us who we. The game will be opened inside a popup window. please turn off any block popup program before clicking on the play button. Here, we show that human memory b lymphocytes. proliferate and differentiate into plasma cells in response to polyclonal stimuli, .. Is human memory similar to to the ram in a pc? discussion by michael freed, aerospace human factors, nasa ames research center. Exploiting human memory to create secure and emorable passwords they are difficult to remember because there are limits to human memory. Basic perofrmance data were obtained on the effect of critical task variables in unaided multiobject tracking behavior. .forgetmenot. intimate computing in support of human memory. a prototype device for memory support, an example of contextbased ...
B-cells go through a variety of stages during their development which is discussed in B-cell Development. However, the major functionally important stages to be aware of are the final stages known as the Plasma Cell and Memory B Cell stages. Plasma Cells are B-cells specialized for high levels of antibody synthesis and secretion. Memory B Cells are quiescent antigen-sepecific cells which differentiate following a primary immune response to a particular microbe which can become rapidly activated to differentiate into Plasma Cells if the microbe if re-encountered. B-cells can also be classified based on the subtype of antibody which they secrete ...
The B cell receptor (BCR) consists of an antigen-binding membrane immunoglobulin (mIg) associated with the CD79α and CD79β heterodimer. Naïve B cells express the IgM and IgD isotypes, which have very short cytoplasmic tails and therefore depend on CD79α and CD79β for signal transduction. After antigenic stimulation, B cells undergo isotype switching to yield IgG, IgE, or IgA. Recent research suggests that the ability of the B cell coreceptor CD22 to regulate BCR signaling depends on the isotype of the mIg cytoplasmic tail. Cell lines that express a BCR with the cytoplasmic tail from IgG, the isotype found in memory B cells, are not subject to CD22 regulation, whereas cell lines that express BCRs with IgM cytoplasmic tails are subject to CD22 regulation. Moreover, stimulation through BCRs containing an IgG cytoplasmic tail causes increased numbers of antigen-specific clones to accumulate. These observations are a valuable step toward understanding the difference in B cell signaling between ...
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As there is growing evidence for the tumor microenvironments (TME) role in tumorigenesis, we investigated the role of fibroblast-expressed kinases in triple negative breast cancer (TNBC). Using a high-throughput kinome screen combined with 3D invasion assays, we identified fibroblast-expressed PIK3Cδ (f-PIK3Cδ) as a key regulator of progression. Although PIK3Cδ was expressed in primary fibroblasts derived from TNBC patients, it was undetectable in breast cancer cell lines. Genetic and pharmacologic gain- and loss-of functions experiments verified the contribution of f-PIK3Cδ in TNBC cell invasion. Integrated secretomics and transcriptomics analyses revealed a paracrine mechanism via which f-PIK3Cδ confers its pro-tumorigenic effects. Inhibition of f-PIK3Cδ promoted the secretion of factors, including PLGF and BDNF, which led to upregulation of NR4A1 in TNBC cells where it acts as a tumor suppressor. Inhibition of PIK3Cδ in an orthotopic BC mouse model reduced tumor growth only after ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
Combination checkpoint blockade (CCB) targeting inhibitory CTLA4 and PD1 receptors holds promise for cancer therapy. Immune-related adverse events (IRAEs) remain a major obstacle for the optimal application of CCB in cancer. Here, we analyzed B cell changes in patients with melanoma following treatment with either anti-CTLA4 or anti-PD1, or in combination. CCB therapy led to changes in circulating B cells that were detectable after the first cycle of therapy and characterized by a decline in circulating B cells and an increase in CD21lo B cells and plasmablasts. PD1 expression was higher in the CD21lo B cells, and B cell receptor sequencing of these cells demonstrated greater clonality and a higher frequency of clones compared with CD21hi cells. CCB induced proliferation in the CD21lo compartment, and single-cell RNA sequencing identified B cell activation in cells with genomic profiles of CD21lo B cells in vivo. Increased clonality of circulating B cells following CCB occurred in some patients. ...
AC50 of HA-specific GC B cells can be used as a proxy for population avidity to PR8 HA. (A) Overview of AC50. GC B cell rHA staining frequency is plotted agains
Our immune system efficiently protects us against the daily onslaught of foreign pathogens. However, the targeting, strength and extent...
The main cell types in humoral immune responses is B cell which acts as antigen presenting cells, developing antibodies to fight foreign antigens and prepare plasma cells or memory B cells. Isolation of B cells can be done with the help of the cell isolation kits which are fast and easy to isolate highly and purified functional B cells for research purpose. StemCell organization helps to make these researches successful with their high quality product, providing consistent as well as all the required support from their dedicated staff available on board. They have also received the ISO certificate for research, manufacturing and shipping facilities to France, Canada, USA, to name a few ...
Researchers at the University of Gothenburgs Sahlgrenska Academy have looked at B cells, a type of white blood cell that produces antibodies that can protect the body against infection and play a key role in the development of allergies. By studying 65 healthy newborn babies in the Västra Götaland region, researcher Anna-Carin Lundell and her colleagues were able to show that infants whose gut is colonised by E. coli bacteria during the first few weeks of life had a higher number of memory B cells at the age of both four and 18 months ...
Reimer, D and Lee, AYS and Bannan, J and Fromm, P and Kara, EE and Comerford, I and McColl, S and Wiede, F and Mielenz, D and Korner, H, Early CCR6 expression on B cells modulates germinal centre kinetics and efficient antibody responses, Immunology and Cell Biology, 95, (1) pp. 33-41. ISSN 0818-9641 (2017) [Refereed Article ...
A type of white blood cell that matures in the bone marrow. B cells make antibodies against antigens and create new B cells that remember the antigens old B cells already encountered ...
Distinguishing between human lymphocyte subsets. Identification of cone cell inner and outer segments and to a lesser extent ...
el-Demiry M, James K (1988). "Lymphocyte subsets and macrophages in the male genital tract in health and disease. A monoclonal ... effects of activin and transforming growth factor beta on lymphocyte subsets in vitro". Biology of Reproduction. 58 (4): 943- ... T-lymphocytes (T-cells) are white blood cells which take part in cell-mediated immunity. They are often found within tissues ... B-lymphocytes take part in the adaptive immune response and produce antibodies. These cells are not normally found in the ...
Huang W, Berube J, McNamara M, Saksena S, O'Gorman M (2020). "Lymphocyte Subset Counts in COVID-19 Patients: A Meta-Analysis". ... revealed that another T helper subset may exist. Th9 cells are claimed to be an IL9 (interleukin 9)-producing T cell subset ... "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions". Nature. 401 (6754): 708-712. doi: ... These effects are primarily due to the loss of any helper T cell that can interact with the B lymphocyte correctly. Another ...
Poussier, P., et al., Lymphopenia and abnormal lymphocyte subsets in the "BB" rat: relationship to the diabetic syndrome. ...
Harris MT, Schwarting GA, Stout RD (September 1981). "Selective expression of asialo GM1 on maturational subsets of lymphocytes ... 15 July 1997). "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) ... in vivo differentiation into lymphocytes and potential for germ line transmission". FEBS Letters. 455 (1-2): 101-4. doi:10.1016 ...
"Transitional Type 1 and 2 B Lymphocyte Subsets Are Differentially Responsive to Antigen Receptor Signaling". J. Biol. Chem. 277 ... Chung JB, Sater RA, Fields ML, Erikson J, Monroe JG (2002). "CD23 defines two distinct subsets of immature B cells which differ ... T1 B cells are distinguished from the other subsets by the following surface marker characteristics: they are IgMhiIgD−CD21− ... 2010). "Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B ...
He was among those who defined the subsets of human B lymphocytes. These studies led to a redefinition of many human B cell ... "Analysis of somatic mutation in five B cell subsets of human tonsil". The Journal of Experimental Medicine. 180 (1): 329-339. ... "Receptor revision of immunoglobulin heavy chain variable region genes in normal human B lymphocytes". The Journal of ...
1996;17(2):197-9. PMID 8936281 Differences in intraepithelial lymphocyte t cell subsets isolated from murine small versus large ... his research was on experimental models of hapten-induced Inflammatory bowel disease and studying intraepithelial lymphocytes. ...
Sallusto, F.; Lenig, D.; Förster, R.; Lipp, M.; Lanzavecchia, A. (1999). "Two subsets of memory T lymphocytes with distinct ... Viola, A.; Schroeder, S.; Sakakibara, S.; Lanzavecchia, A. (1999). "T lymphocyte costimulation mediated by reorganization of ... lymphocyte activation and immunological memory and iii) human monoclonal antibodies. In 1985, using antigen-specific T and B ... and discovered a fundamental division of memory T cells into two major subsets of central memory and effector memory and ...
"Two subsets of memory T lymphocytes with distinct homing potentials and effector functions." Nature 401.6754 (1999): 708.. ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... These two subsets were previously called "naturally occurring" and "adaptive" (or "induced"), respectively. Both subsets ... Gamma delta T cells (γδ T cells) represent a small subset of T cells which possess a γδ TCR rather than the αβ TCR on the cell ... A T cell is a type of lymphocyte. T cells are one of the important white blood cells of the immune system and play a central ...
... s are a subset of T lymphocytes that might have some of the same functions as memory B cells. Their lineage is ... Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (October 1999). "Two subsets of memory T lymphocytes with distinct ... Those lymphocytes are capable of self-renewal as are the TCM lymphocytes and are also capable of generating both the TCM and ... TCM : TCM lymphocytes have several attributes in common with stem cells, the most important being the ability of self-renewal, ...
885-895 Han S, Dillon SR... Kelsoe G (1997). V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. ... Kelsoe's research focuses on lymphocyte development and activation, as well as how lymphocytes specific to certain pathogens ...
This selective inhibition is not observed in conventional chemotherapy which reduces the number of all lymphocyte subsets. In ...
Knockout mice that lack TOX have a severe defect in development of certain subsets of T lymphocytes. In cancer or during ... Patients with cancer typically have high levels of TOX in their tumor-infiltrating lymphocytes, and anti-tumor immunity is ... TOX is highly expressed in the thymus, the site of development of T lymphocytes. ...
"Distribution of cyclophilin B-binding sites in the subsets of human peripheral blood lymphocytes". Immunology. 91 (4): 609-17. ... This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. ... recognized by HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes which could be used as cancer vaccines, and in fact ...
Effects of Lycium barbarum polysaccharide on tumor microenvironment T-lymphocyte subsets and dendritic cells in H22-bearing ...
Nassar GM, Montero A, Fukunaga M, Badr KF (1997). "Contrasting effects of proinflammatory and T-helper lymphocyte subset-2 ... and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. In skin, Langerhans cells strongly ... "Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes". Eur. J. Biochem. 232 (1): 37-46. doi: ...
In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding. Diversity is ... T and B lymphocytes are the cells of the adaptive immune system. The human body has about 2 trillion lymphocytes, which are 20- ... Lymphocyte receptors, Ig and TCR, are found in all jawed vertebrates. The most ancient Ig class, IgM, is membrane-bound and ... Cytotoxic T cells (also known as TC, killer T cell, or cytotoxic T-lymphocyte (CTL)) are a sub-group of T cells that induce the ...
There are no major changes in the T-lymphocytes subsets of the mesenterial lymph node. Instead γδ-T cells guide the immune ... Antigens of C. oncophora larvae and adult worms are capable of triggering lymphocyte proliferation. Moreover, excretory/ ... Primary infection does not involve recruitment of specific lymphocytes to the intestinal mucosa. ...
PTSAgs induce the VB-specific expansion of both CD4 and CD8- subsets of T Lymphocytes. TSST-1 forms homodimers in most of its ...
A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes". Journal of ... Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several ... "Phenotypic and functional analysis of CD4+ NKRP1A+ human T lymphocytes. Direct evidence that the NKRP1A molecule is involved in ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... revealed that another T helper subset may exist. Th9 cells are claimed to be an IL9 (interleukin 9)-producing T cell subset ... Th17 helper cells are a subset of T helper cells developmentally distinct from Th1 and Th2 lineages producing interleukin 17 ( ... For example, the Th2 cytokine IL-10 inhibits cytokine production of both Th subsets in humans. Human IL-10 (hIL-10) suppresses ...
Sallusto F, Lenig D, Förster R, Lipp M, Lanzavecchia A (1999). "Two subsets of memory T lymphocytes with distinct homing ... A T cell, or T lymphocyte, is a type of lymphocyte (a subtype of white blood cell) that plays a central role in cell-mediated ... These two subsets were previously called "naturally occurring", and "adaptive" or "induced", respectively.[13] Both subsets ... within a population of lymphocytes known as intraepithelial lymphocytes. In rabbits, sheep, and chickens, the number of γδ T ...
2001a;97:319-327 Lymphocyte phenotype subsets in the cerebrospinal fluid of normal horses and horses with equine protozoal ...
NK Cells are not a subset of the T lymphocyte family. Natural cytotoxicity receptors directly induce apoptosis after binding to ... granular lymphocytes known today as NK cells. The demonstration that density gradient-isolated large granular lymphocytes were ... Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. The role NK cells ... A functional marker of human non-T lymphocytes". Clinical and Experimental Immunology. 21 (2): 226-35. PMC 1538269. PMID 810282 ...
... differential signaling requirements for activation of assembled cyclin D3-cdk4 complexes in B-1 and B-2 lymphocyte subsets". ...
Ademmer; Ebert; Müller-Ostermeyer; Friess; Büchler; Schubert; Malfertheiner (April 1998). "Effector T lymphocyte subsets in ... 2006). "Profiling lymphocyte subpopulations in peripheral blood under efalizumab treatment of psoriasis by multi epitope ligand ... "Automatic Recognition of Muscle-Invasive T-Lymphocytes Expressing Dipeptidyl-Peptidase IV (CD26) and Analysis of the Associated ... "Poisson Numbers and Poisson Distributions in Subset Surprisology". Annals of Combinatorics. 8 (4): 473-485. doi:10.1007/s00026- ...
"In Vitro Measles Virus Infection of Human Lymphocyte Subsets Demonstrates High Susceptibility and Permissiveness of both Naive ... In the later stages of infection, the virus infects other immune cell types, including B cells and T lymphocytes also via ... Three receptors for the H protein have been identified to date: complement regulatory molecule CD46, the signaling lymphocyte ...
... these specific transcription factors activate or repress target genes critical in the differentiation of the lymphocyte subsets ... Studies have confirmed the ability of some ILC subsets to convert into a different subset in the presence of specific cytokines ... There are two subsets of ILC3s, NCR- and NCR+ ILC3s, with the displayed NCR on mice ILC3s being NKp46, in comparison to NKp44 ... All ILC subsets are present in the liver and regulate the immune response to protect the tissue from viral and bacterial ...
Sallusto, F.; Lenig, D.; Förster, R.; Lipp, M.; Lanzavecchia, A. Two subsets of memory T lymphocytes with distinct homing ... T细胞(英語:T cell、T lymphocyte)是淋巴细胞的一种,在免疫反應中扮演着重要的角色。T是胸腺(thymus)而不是甲狀腺(thyroid)的英文缩写。T细胞在骨髓被製造出來之後,在胸腺内進行「新兵訓練」分化成熟為不同亚型的效应T細胞,成 ... CD4+ T cells are required for secondary expansion and memory in CD8+ T lymphocytes. Nature. February
It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... subset panel illustrates a schematic view of CD20 structure and rituximab.[35] ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] ...
It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... subset panel illustrates a schematic view of CD20 structure and rituximab.[36] ... "B-Lymphocyte Depletion in Patients With Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Randomized, Double-Blind, Placebo ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] This also includes ...
Determination of T-lymphocyte subsets on site in rural Tanzania: results in HIV-1 infected and non-infected individuals. ... Analysis of blood lymphocyte subsets in children living on territory that received high amounts of fallout from Chernobyl ... Correlation of CD8 lymphocyte activation with cellular viremia and plasma HIV RNA levels in asymptomatic patients infected by ... The staging and prognostic value of subset markers on CD8 cells in HIV disease. In Janossy G, Autran B. Miedema F (eds): ...
Lymphocyte. Main article: Lymphocyte. Lymphocytes are much more common in the lymphatic system than in blood. Lymphocytes are ... The complete blood cell count is a blood panel that includes the overall WBC count and various subsets such as the absolute ... Lymphocyte. 30%. Small lymphocytes 7-8. Large lymphocytes 12-15. *B cells: releases antibodies and assists activation of T ... lymphocytes) by hematopoietic lineage (cellular differentiation lineage).[6] Lymphocytes can be further classified as T cells, ...
Another example occurs in activated T cell lymphocytes, i.e., when a T cell is induced to mature by binding to a peptide:MHC ... FGF9 and their receptors encompass a growth factor autocrine loop that is active in a subset of gefitinib-resistant NSCLC cell ... "Neuregulin-1-Mediated Autocrine Signaling Underlies Sensitivity to HER2 Kinase Inhibitors in a Subset of Human Cancers". Cancer ...
However, the various subsets may also be considered part of the innate immune system where a restricted TCR or NK receptors may ... One can see red blood cells, several knobby white blood cells including lymphocytes, a monocyte, a neutrophil, and many small ... Like other 'unconventional' T cell subsets bearing invariant T cell receptors (TCRs), such as CD1d-restricted Natural Killer T ... Invertebrates do not possess lymphocytes or an antibody-based humoral immune system, and it is likely that a multicomponent, ...
Yamada H, Yamada K, Waki M, Umegaki K (October 2004). "Lymphocyte and plasma vitamin C levels in type 2 diabetic patients with ... Another subset of trials looked at therapeutic use, meaning that vitamin C was not started unless the people started to feel ... Emadi-Konjin P, Verjee Z, Levin AV, Adeli K (May 2005). "Measurement of intracellular vitamin C levels in human lymphocytes by ... A subset of trials reported that supplementation reduced the incidence of colds by half in marathon runners, skiers, or ...
For virus-associated tumors, such as cervical cancer and a subset of head and neck cancers, epitopes derived from viral open ... They become activated and start to secrete cytokines, substances that activate cytotoxic T lymphocytes (CTL), antibody- ... Cytotoxic T lymphocytes that recognize these antigens may be able to destroy tumor cells.[15] ... to assess reactivity in patients treated by either tumor-infiltrating lymphocyte (TIL) cell therapy or checkpoint blockade. ...
PrP immune cells include hematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it ... attenuated the epilepsy-induced death phenotype seen in a subset of these animals.[54] Taken collectively, recent evidence ...
... causes a response in a type of immune cell called a TH2 lymphocyte, which belongs to a subset of T cells that produce a ... lymphocytes, eosinophils, and macrophages to the initial site. The reaction is usually seen 2-24 hours after the original ... cytokine called interleukin-4 (IL-4). These TH2 cells interact with other lymphocytes called B cells, whose role is the ...
... s are a subset of cytokines that are produced by a type of immune cell known as a lymphocyte.[1] They are protein ... Circulating lymphocytes can detect a very small concentration of lymphokine and then move up the concentration gradient towards ... Lymphokines have many roles, including the attraction of other immune cells, including macrophages and other lymphocytes, to an ...
Many cells of the immune system are required for this process, including lymphocytes (T-cells and B-cells) and antigen ... These antibodies can be subdivided according to their specificity, and each subset has different propensities for specific ... In normal physiology, lymphocytes that recognise human proteins (autoantigens) either undergo programmed cell death (apoptosis ... This self-tolerance means that lymphocytes should not incite an immune response against human cellular antigens. Sometimes, ...
The rare disorder X-linked neutropenia has also been linked to a specific subset of WAS mutations.[9] ... and genomic instability in lymphocytes". The Journal of Experimental Medicine. 207 (6): 1145-52. doi:10.1084/jem.20091245. PMC ...
... discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes". The ... "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway". The ...
Nassar GM, Montero A, Fukunaga M, Badr KF (1997). "Contrasting effects of proinflammatory and T-helper lymphocyte subset-2 ... and B-lymphocytes express ALOX5. Platelets, T cells, and erythrocytes are ALOX5-negative. In skin, Langerhans cells strongly ... "Studies on the regulation and localization of 5-lipoxygenase in human B-lymphocytes". Eur. J. Biochem. 232 (1): 37-46. doi: ...
Specific acquired immunity against infectious diseases may be mediated by antibodies and/or T lymphocytes. Immunity mediated by ... a contagious disease is a subset of infectious disease that is especially infective or easily transmitted. Other types of ... or by T lymphocytes, which will kill a cell parasitized by a microorganism. ...
Histologic findings of a deep mixed infiltrate with lymphocytes, plasma cells, and neutrophils (with or without eosinophils) ... found an association between a polymorphism in the TNF-alpha gene and risk for oral LP in a subset of patients. An Italian ...
IELs: intraepithelial lymphocytes). *^ a b Lionetti E, Francavilla R, Pavone P, Pavone L, Francavilla T, Pulvirenti A, Giugno R ... "In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of ... Therefore, these forms of the receptor are more likely to activate T lymphocytes and initiate the autoimmune process.[28] ... Biopsy of small bowel showing coeliac disease manifested by blunting of villi, crypt hypertrophy, and lymphocyte infiltration ...
Dermatographism is the most common form of a subset of chronic hives, acknowledged as "physical hives".[citation needed] ... Hydroxychloroquine is an antimalarial agent that suppresses T lymphocytes. It has a low cost however it takes longer than ...
In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding.[33] Diversity is ... LymphocytesEdit. Main article: Lymphocyte. T and B lymphocytes are the cells of the adaptive immune system. The human body has ... CD8+ T lymphocytes and cytotoxicityEdit. Main article: Cytotoxic T cell. Cytotoxic T cells (also known as TC, killer T cell, or ... The cells that carry out the adaptive immune response are white blood cells known as lymphocytes. B cells and T cells, two ...
lymphocyte migration. • T cell migration. • thymocyte migration. • DN2 thymocyte differentiation. • DN3 thymocyte ... NKT cell subsets with distinct cytokine-producing capacity". Blood. 100 (1): 11-6. doi:10.1182/blood-2001-12-0196. PMID ... 1997). "Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC". J. Biol. Chem. 272 ( ... "Up-regulation of CCR5 and CCR6 on distinct subpopulations of antigen-activated CD4+ T lymphocytes". J. Immunol. 168 (1): 65-72 ...
Phenotypically distinct subsets of CD4+ T cells induce or protect from chronic intestinal inflammation in C. B-17 scid mice. „ ... A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. „J Exp Med". 144 ... I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause ... OX-22high CD4+ T cells induce wasting disease with multiple organ pathology: prevention by the OX-22low subset. „J Exp Med". ...
The approval is for a subset of kidney cancer patients who are at intermediate risk for disease recurrence. It awaits approval ... Shankaran V, Ikeda H, Bruce AT, White JM, Swanson PE, Old LJ, Schreiber RD (2001-04-26). "IFNgamma and lymphocytes prevent ...
Hence one could choose any subset of the equations, with the number equal to the number of variables, and solve them. But, when ... Low persistence of micronucleus frequency in lymphocytes of individuals after cardiac scan". Mutat. Res. Fundam. Mol. Mech. ... a classifier or more basic algorithm is trained to distinguish trials for different conditions within a subset of the data. The ... "Genotoxic effects of 3 T magnetic resonance imaging in cultured human lymphocytes". Bioelectromagnetics. 32 (7): 535-42. doi: ...
"Lymphocyte activation gene-3 expression defines a discrete subset of HIV-specific CD8+ T cells that is associated with lower ... "Entrez Gene: LAG3 lymphocyte-activation gene 3".. *^ a b c Triebel F, Jitsukawa S, Baixeras E, Roman-Roman S, Genevee C, Viegas ... Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene.[5] LAG3, which was ... "T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of ...
Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ... immunohistochemical characterization of four thymic epithelial subsets defined by monoclonal anti-keratin antibodies., Eur J ... Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ... P.G. Isaacson, A.J. Norton, B.J. Addis, THE HUMAN THYMUS CONTAINS A NOVEL POPULATION OF B LYMPHOCYTES, The Lancet, 330. ...
"Lymphocyte subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow ... Lymphocyte development, veebiversioon (vaadatud 27.02.2014) (inglise keeles). *The Development and Survival of Lymphocytes, ... Alberts B, Johnson A, Lewis J, et al., Lymphocytes and the Cellular Basis of Adaptive Immunity, Molecular Biology of the Cell, ... Janeway CA Jr, Travers P, Walport M, et al., Generation of lymphocytes in bone marrow and thymus, "Immunobiology: The Immune ...
... including mesenchymal stem cells and regulatory T-lymphocytes. The mesenchymal stem cells thus alter the outcome of the immune ... response by changing the cytokine secretion of dendritic and T-cell subsets. This results in a shift from a pro-inflammatory ...
T-lymphocyte subsets in acute illness.. Feeney C1, Bryzman S, Kong L, Brazil H, Deutsch R, Fritz LC. ... but no relationship was found between total lymphocyte or lymphocyte subset counts and APACHE II score, predicted mortality ... To determine the range of T-lymphocyte subsets (CD4, CD8, and CD4/CD8 ratios) in acutely ill, hospitalized patients and to ... predicted mortality rate with T-lymphocyte subsets.. SETTING: Urban county hospital intensive care unit (ICU), serving as the ...
... Tim R Mosmann, University of Rochester Medical Center, Rochester, New York, USA Jonathan ... 1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401 (6754): 708-712. ... Mosmann, Tim R, and Rebhahn, Jonathan A(Nov 2016) T Lymphocytes: Plasticity of Subsets. In: eLS. John Wiley & Sons Ltd, ... 1990) Evidence for compartmentalization of functional subsets of CD2+ T lymphocytes in atopic patients. Journal of Immunology ...
T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell ... Correlations between T-lymphocyte subset values and stages or cell types of disease were sought. Total lymphocytes in the ... T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell ... Previous Document: Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patien.... ...
To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with ... shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. ... Blood was taken for serum oestradiol, lymphocyte counts and lymphocyte subset counts (CD2+, CD4+, CD8+ and B cells) before ... Lymphocyte subsets in patients with oestrogen deficiency J Reprod Immunol. 1991 May;20(1):85-91. doi: 10.1016/0165-0378(91) ...
Reference values of lymphocyte subsets in healthy, HIV-negative children in Cameroon.. Sagnia B1, Ateba Ndongo F, Ndiang Moyo ... Normal lymphocyte subsets values among children from Cameroon differ from reported values in Caucasian and some African ... Lymphocyte subset reference values used to monitor infectious diseases, including HIV/AIDS, tuberculosis, malaria, or other ... We evaluated about 23 different lymphocyte subsets in which the absolute number and percentage values differed significantly (P ...
... Jue Lin,1 Joshua Cheon,1 Rashida Brown,2 ...
Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets.. T A Barrett, T F Gajewski, D Danielpour, E B Chang, K ... Differential function of intestinal intraepithelial lymphocyte subsets. Message Subject (Your Name) has forwarded a page to you ...
Dumitriu I.E., Kaski J.C. (2013) The Role of Lymphocytes in the Pathogenesis of Atherosclerosis: Focus on CD4+ T Cell Subsets. ... The Role of Lymphocytes in the Pathogenesis of Atherosclerosis: Focus on CD4+ T Cell Subsets. ... Functional subsets of CD4 T cells in rheumatoid synovitis. Arthritis Rheum. 1998;41(12):2108-16.PubMedCrossRefGoogle Scholar ... Acute Coronary Syndrome Th17 Cell Atherosclerotic Plaque Treg Cell Cell Subset These keywords were added by machine and not by ...
Lymphocytes and their subsets, NK cells were signed and measured by flow cytometric immunophenotyping in Coulter EPIX XL with ... In the course of active infection B.burgdorferi essential changes in lymphocytes subsets are observed. Decreased percentages of ... subsets CD4, CD8, NK cells, activated lymphocytes with fenotype CD3+HLA-DR, CD4/CD8 rate in Lyme borreliosis was carried out ... Percentage CD4 in II examination (x = 42) was lower than control (x = 45.8). Subset of CD8 had lower value in I examination (x ...
T Lymphocyte Subsets in Patients with Bronchiectasis B.C. Leahy B.C. Leahy ... B.C. Leahy, M.A. Horan, T.B. Stretton; T Lymphocyte Subsets in Patients with Bronchiectasis. Clin Sci (Lond) 1 February 1984; ...
T-Lymphocyte Subsets in Adult Coeliac Disease G. R. Corazza; G. R. Corazza ... G. R. Corazza, P. Tabacchi, M. Frisoni, M. Londei, D. Bastia, G. Gasbarrini; T-Lymphocyte Subsets in Adult Coeliac Disease. ...
Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ... TI - Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B ... Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ... Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells ...
Ana A. Weil, Mohammad Arifuzzaman, Taufiqur R. Bhuiyan, Regina C. LaRocque, Aaron M. Harris, Emily A. Kendall, Azim Hossain, Abdullah A. Tarique, Alaullah Sheikh, Fahima Chowdhury, Ashraful I. Khan, Farhan Murshed, Kenneth C. Parker, Kalyan K. Banerjee, Edward T. Ryan, Jason B. Harris, Firdausi Qadri, Stephen B. Calderwood ...
Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients ... Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a ... Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B ... LU, Zhimin et al. Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections. ...
Distribution of lymphocyte subsets. The lymphocyte subset results of these three groups including HC, non-infection SLE ... The percentages and counts of the lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+ ... Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B ... Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients ...
This study investigated the effects of a temporally confined naturalistic stressor (academic stress) on immune functions. Furthermore, moderating influences of a number of psychological variables were assessed. Five blood samples were obtained from 20 students during an observation period of 8 weeks, starting 4.5 weeks before an exam period up to 1 week following the last exam. The analysis of 45 immune parameters revealed several time-dependent changes attributable to examination stress. We observed a reduction in the absolute numbers of natural killer (NK) cells and monocytes in peripheral blood and a shift towards more immature and naïve cells within NK and T cell populations. In addition, IL-6 and TNF-α production by LPS-stimulated monocytes was increased. Psychological variables were grouped by means of factor analyses into two factors. One factor, which was interpreted as an indication of chronic stress, moderated the relationships between academic stress and percentages of mature CD57+ NK cells
Hepatitis C virus (HCV) in lymphocyte subsets and in B lymphocytes expressing rheumatoid factor cross-reacting idiotype in type ... in lymphocyte subsets and in B lymphocytes expressing rheumatoid factor cross-reacting idiotype in type II mixed ... B-lymphocytes are predominantly involved in viral propagation of hepatitis C virus (HCV). Arch Virol (Suppl) 1994; 9: 307-16.. ... Evidence of selective expansion of a B cell subset characterized by the expression of cross-reactive idiotypes. Arthritis Rheum ...
This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other ... Moroccan lymphocyte subsets reference ranges: age, gender, ethnicity, and socio-economic factors dependent differences.. *. ... This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. These results can be used for other ...
Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. ... Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. ...
... ... Kj Blank, Rd Mulero-Marchese, and Tg Sieck, "Strain-dependent migration of lymphocytes to the vaginal mucosa after peripheral ...
These molecules are essential markers for the identification and isolation of leukocytes and lymphocyte subsets. Here, we ... Using the resource, we examined differentially expressed proteins between granulocyte, monocyte and dendritic cell subsets, and ... Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, ... and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. ...
The present study aimed to evaluate the distribution of major lymphocyte subsets and cytokine expression profiles in the ... blood lymphocyte subpopulations, and serum cytokines, respectively. The FA-exposed workers were divided into low and high ... Though the mechanisms by which FA affects blood lymphocytes and/or lymphocyte subsets are still unknown, the results from ... Major lymphocyte subsets were analyzed on the same day. Serum was separated within 4 h after collection, put into 1.5 mL tubes ...
Secondary: To compare, the absolute numbers and percentages of lymphocytes subsets as measured in the Sysmex-XN 20 analyser to ... An Observational Study Exploring the Utility of the Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological ... Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological Parameters in Chronic/Acute Viral Infections (SASA) ... Sysmex-XN 20 Analyser to Assess Lymphocyte Subsets and Other Haematological Parameters in Chronic/Acute Viral Infections ...
Transmigration of lymphocyte subsets across these barriers and their activation/differentiation within the periphery and ... and enables simulation-based prediction of distribution and differentiation stages of lymphocyte subsets in the case of limited ... correctly reproduces acquired data on location and differentiation states of distinct lymphocyte subsets under homeostatic and ... are warranted that qualitatively and quantitatively predict the distribution and differentiation stages of lymphocyte subsets ...
Alcohol use is known to alter immune function and has immunosuppressive effects that may modify T-lymphocyte subpopulations. ... Crum, R., Galai, N., Cohn, S., Celentano, D., & Vlahov, D. (1996). Alcohol Use and T-Lymphocyte Subsets among Injection Drug ... Alcohol Use and T-Lymphocyte Subsets among Injection Drug Users with HIV-1 Infection: A Prospective Analysis. ... Alcohol Use and T-Lymphocyte Subsets among Injection Drug Users with HIV-1 Infection: A Prospective Analysis ...
This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary ... T-lymphocytes), CD19(+) (B-lymphocytes), CD4(+) (helper/inducer lymphocytes) and CD8(+) (suppressor/cytotoxic lymphocytes) ... This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary ...
Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression.. A J Schlueter, T R Malek, C ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ... Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. ...
Lymphocyte subsets and ocular inflammation: future prospects for immune deviation therapy?. British Journal of Ophthalmology ...
Peripheral blood lymphocytes study. Lymphocyte subsets were studied by three-color flow cytometry in whole blood after staining ... The present study confirms that endometrium presents a wide spectrum of T and NK lymphocyte subsets. Endometrial T lymphocytes ... In this study we aimed to make the first step to compare the levels of T and NK lymphocyte subsets in endometrium and ... Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood. Viktor P. Chernyshov ...
  • Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. (muni.cz)
  • High performance liquid chromatography, flow cytometry, and cytometric bead array were used to analyze FA in air sample and formic acid in urine, blood lymphocyte subpopulations, and serum cytokines, respectively. (plos.org)
  • Objectives: Alcohol use is known to alter immune function and has immunosuppressive effects that may modify T-lymphocyte subpopulations. (rti.org)
  • If evidence for such an association could be found, it would suggest a direct or indirect (via DC) immunoregulatory conversation of these two lymphocyte subpopulations. (harvest-re.com)
  • We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. (biomedcentral.com)
  • Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples. (biomedcentral.com)
  • Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. (biomedcentral.com)
  • The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. (biomedcentral.com)
  • The numbers and the distribution of T-lymphocyte subpopulations in lymph node granulomas from 11 patients with sarcoidosis were studied in cryostat sections by an immunoperoxidase technique. (semanticscholar.org)
  • Peripheral blood lymphocyte subpopulations and self-reported stress (Perceived Stress Scale) were measured before training and 1-2 months later as exams approached. (isharonline.org)
  • These results demonstrate that there is significant abnormality in the subpopulations of lymphocytes in patients with sudden hearing loss, suggesting the immune system as a possible etiopathogenic factor in this entity. (kjorl.org)
  • Lymphocyte subpopulations were quantified by immunofluorescence using monoclonal antibodies against total T-cells, T-helper and T-suppressor cells. (medicdebate.org)
  • The counts of blood leukocytes, lymphocytes and lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD16+CD56+) were determined both on admission and seven days later using a flow cytometry. (hacettepe.edu.tr)
  • When adjusting for sex, current breastfeeding status, dehydration, and nutritional status of children, lymphocyte subpopulations did not differ significantly between the glutamine- and placebo-supplemented groups on the 7(th) day of intervention. (hacettepe.edu.tr)
  • Each of these lymphocyte subpopulations have distinct effector and regulatory functions and are maintained in homeostasis under normal physiological conditions. (testcatalog.org)
  • Oral lichen planus and chronic junctional stomatitis: differences in lymphocyte subpopulations. (semanticscholar.org)
  • We mainly investigated the major lymphocyte subsets in untreated SLE patients and analyzed their relation to infections. (scielo.br)
  • The present study aimed to evaluate the distribution of major lymphocyte subsets and cytokine expression profiles in the peripheral blood of FA-exposed workers. (plos.org)
  • Results: The total lymphocyte count (p=0.00044) and three of the four major lymphocyte subsets (ie, CD4+ T cells (p=0.00019), CD8+ T cells (p=0.0058) and B cells (p=0.017)) were higher in exposed versus control workers and findings were highly consistent when stratified by smoking status. (elsevier.com)
  • These results showed that early-life environmental PCB exposure was associated with fluctuations in major lymphocyte subsets in children, suggesting that there is a post-natal immune system response to PCB exposures. (elsevier.com)
  • As Glut1 is upregulated on tumor cells in response to hypoxia, we assessed whether surface Glut1 levels regulate the antigen responsiveness of human T lymphocytes in both hypoxic and atmospheric oxygen conditions. (cnrs.fr)
  • Furthermore, TCR-stimulated human T lymphocytes sorted on the basis of Glut1-Lo and Glut1-Hi profiles maintained distinct characteristics, irrespective of the oxygen tension. (cnrs.fr)
  • Thus, Glut1 surface levels identify human T lymphocytes with distinct effector functions in both hypoxic and atmospheric oxygen tensions. (cnrs.fr)
  • Two subsets of human T lymphocytes expressing gamma/delta antigen receptor are identifiable by monoclonal antibodies directed to two distinct molecular forms of the receptor. (rupress.org)
  • A small percentage of human T lymphocytes, predominantly CD8 + T cells, express receptors for HLA class I molecules of natural killer type (NK-R) that are inhibitory for T-cell antigen receptor (TCR)-mediated functions. (elsevier.com)
  • We have attached antibody-coupled magnetic microspheres to the surface of human T-lymphocytes before performing various assays. (aaccjnls.org)
  • Acute illness alone, in the absence of HIV infection, can be associated with profound decreases of T-lymphocyte populations. (nih.gov)
  • In the course of active infection B.burgdorferi essential changes in lymphocytes subsets are observed. (prohealth.com)
  • Monitoring lymphocyte subsets, especially CD4+T cells, may be helpful for identifying the presence of infection in SLE patients. (scielo.br)
  • As we know, peripheral lymphocytes play an important role in anti-infection immune response and abnormal status accounts for a bad ending in infection diseases ( 7 ). (scielo.br)
  • Methods: Using prospective data from a cohort of IDUs in a study of the natural history of HIV infection, we examined the relationship of alcohol use and changes in T-lymphocyte subsets. (rti.org)
  • Measles virus (MV) infection of immune cells is mediated by the cellular receptor CD150, expressed by subsets of lymphocytes, dendritic cells, macrophages, and thymocytes. (asm.org)
  • However, limited information is available about the CD150 expression and relative susceptibility to MV infection of B-cell subsets. (asm.org)
  • In this study, we assessed the susceptibility and permissiveness of naive and memory T- and B-cell subsets from human peripheral blood or tonsils to in vitro MV infection. (asm.org)
  • We demonstrated that T H 1T H 17 cells and plasma and germinal center B cells were the subsets most susceptible and permissive to MV infection. (asm.org)
  • Our study suggests that both naive and memory B cells, along with several other antigen-experienced lymphocytes, are important target cells of MV infection. (asm.org)
  • Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node. (asm.org)
  • Peripheral blood lymphocyte, NK cell and activation markers were detected by flow cytometry in renal allograft recipients with (TpC+) or without (TpC-) HCV infection, and compared with age- and sex-matched patients with post-transfusional chronic HCV infection (TfC+) and healthy controls. (hku.hk)
  • Peripheral blood suppressor/cytotoxic T lymphocytes are increased, whereas activated helper/inducer T lymphocytes and NK cells are reduced, in renal allograft recipients with HCV infection. (hku.hk)
  • The aim of this study was to analyze the changes seen in lymphocyte subsets and immunomodulatory cytokine expression in pigs after an acute experimental infection with a European PRRSV field isolate. (surrey.ac.uk)
  • However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection. (biomedcentral.com)
  • All patients transplanted in our institution are routinely evaluated by BAL for detection of opportunistic infection and routine analysis of the various subsets of intra-alveolar cells, particularly lymphocytes. (biomedcentral.com)
  • The most commonly used hematology tests to monitor HIV infection are the complete blood count (CBC) and lymphocyte subsets. (thebody.com)
  • Lymphocytes are the key WBCs involved in immune responses (see CD4 count below) and are often lowered by HIV infection. (thebody.com)
  • 0.0001, respectively) and lymphocyte subsets (T cells, CD4 + and CD8 + T cell subsets) of severe COVID-19 and severe influenza A patients to be significantly lower than those of healthy donors at early infection stages. (researchsquare.com)
  • 1 month) underwent venous blood sampling in order to determinate CD3(+) (T-lymphocytes), CD19(+) (B-lymphocytes), CD4(+) (helper/inducer lymphocytes) and CD8(+) (suppressor/cytotoxic lymphocytes) populations. (biomedsearch.com)
  • One might speculate that graft\specific CD8+ lymphocytes were killed by cytotoxic Treg, thereby preventing increases of CD8+ effector cells and keeping post\transplant CD8+ lymphocyte counts at a stable level. (harvest-re.com)
  • In murine models of CMV pneumonitis, increased numbers of both CD4+ T helper and CD8+ suppressor/cytotoxic pulmonary lymphocytes have been observed [ 6 ]. (biomedcentral.com)
  • Absolute number and percentages of CD3(+)CD4(+) and CD3(+)CD8(+) T lymphocytes, CD3(-)CD56(+) Natural Killer cells (NK cells) and NK cell cytotoxic activity was measured from venous blood. (isharonline.org)
  • We report here the distributions of lymphocyte populations bearing the following antigens: CD3 (T cells), CD19 (B cells), CD4 (T helper/inducer cells), CD8 (T suppressor/cytotoxic and some NK cells), and CD3-, CD16+, and/or CD56+ (NK cells). (ox.ac.uk)
  • To determine the range of T-lymphocyte subsets (CD4, CD8, and CD4/CD8 ratios) in acutely ill, hospitalized patients and to determine whether these concentrations correlate with illness severity, survival rate, or immunodepression. (nih.gov)
  • Blinded human immunodeficiency virus (HIV) and lymphocyte testing was performed on samples from all patients on ICU admission. (nih.gov)
  • T-cell subsets in the peripheral blood of 63 primary lung cancer patients (23 with adenocarcinoma, 23 with squamous cell carcinoma and 17 with small cell carcinoma) and 24 normal healthy controls were determined by indirect immunofluorescence, using the monoclonal antibody reagents OKT3, OKT4 and OKT8. (biomedsearch.com)
  • We have previously shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. (nih.gov)
  • To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with oestrogen deficiency due to other causes. (nih.gov)
  • Blood was taken for serum oestradiol, lymphocyte counts and lymphocyte subset counts (CD2+, CD4+, CD8+ and B cells) before oestrogen replacement in 19 patients with gonadal dysgenesis, 22 patients with hypothalamic-pituitary failure and 24 healthy female control subjects. (nih.gov)
  • The CD4:CD8 ratio in both groups of patients was significantly lower than that in the normal control subjects while the percentages and counts of lymphocytes and CD8+ cells were significantly higher. (nih.gov)
  • Characterization of Lymphocyte Subsets in Patients with common variable immunodeficiency reveals subsets of naive human B cells marked by CD24 expression. (muni.cz)
  • N2 - Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. (muni.cz)
  • We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. (muni.cz)
  • The B cell subsets showed a markedly increased proliferation in CVID 21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. (muni.cz)
  • FO I subset is significantly underrepresented in CVID 21lo patients. (muni.cz)
  • A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression. (muni.cz)
  • The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. (scielo.br)
  • Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. (scielo.br)
  • Lymphocyte subset characterization in patients with early clinical presentation of coronary heart disease. (biomedsearch.com)
  • This study aimed to investigate lymphocyte populations in non-diabetic patients with early clinical presentation of coronary heart disease (CHD). (biomedsearch.com)
  • In patients with recurrent miscarriages, no correlation was found between peripheral and uterine NK cells but an association between peripheral lymphocytes (CD45+CD3-DR+ and CD45+CD3+CD8+DR+) and uterine NK cells was confirmed and it was supposed that T, B and NK lymphocytes provided cytokines for the differentiation of uterus NK cells [8]. (termedia.pl)
  • To evaluate the expression of tumor PD-L1 and changes in tumor-infiltrating lymphocyte (TIL) populations in patients with metastatic melanoma treated with targeted MAPK inhibitors. (aacrjournals.org)
  • BRAFi-treated patients showed significant increases in CD4 + , CD8 + , and PD-1 + lymphocytes from PRE to EDT ( P = 0.001, P = 0.001, P = 0.017, respectively), and Combi-treated patients showed similar increases in CD4 + and CD8 + lymphocytes from PRE to EDT ( P = 0.017, P = 0.021). (aacrjournals.org)
  • Objectives Evaluate the number and distribution of circulating CD4+ T lymphocytes and their CD4+ naïve T cells (T N ), central memory (T CM ), non-terminated effector memory (T NTEM ) and terminated effector memory (T TEM ) T cells subsets in a population of recently diagnosed DMARD naive RA patients before and along the first 6 months of methotrexate (MTX) treatment. (bmj.com)
  • Methods The number of circulating CD4+ T lymphocytes, and of their T N , TM, TCM and TEM subsets in fifty untreated patients with RA before MTX treatment and at 3 and 6 months of treatment were assayed using a multiparametric flow cytometry. (bmj.com)
  • MTX non responder naïve RA patients show from basal conditions a significative expansion of CD4+ T NTEM lymphocytes and develop significant expansion of CD4+ T CM , and CD4+ T EM lymphocyte subsets along MTX treatment. (bmj.com)
  • Conclusions Recently diagnosed DMARD naive RA patients show involvement of the circulating CD4+ T lymphocytes activation/differentiation stage subsets. (bmj.com)
  • MTX treatment show immunomodulatory effects on CD4+ T lymphocyte compartment with different behavior in responder and non-responder patients. (bmj.com)
  • To investigate the change of T-lymphocyte subsets in peripheral blood of rheumatoid arthritis (RA) patients and analyze the effects of Fuzheng Qubi Decoction (FZQBD) on T-cell subsets. (springer.com)
  • Abnormal cellular immunity exists in RA patients, FZQBD could adjust the abnormal T-lymphocyte subsets to normalize it. (springer.com)
  • TfC+ patients and controls had similar numbers and proportions for the lymphocyte subsets and NK cells. (hku.hk)
  • In the present study, we looked for a possible association of NK cells with certain Treg subsets in patients with good long\term renal allograft acceptance. (harvest-re.com)
  • We therefore speculate that whereas healthy controls have stable NK cells counts, NK cell and Treg counts increase with time post\transplant in patients with good graft function and direct or indirect (via DC) conversation of these cell subsets may prevent graft damage by the innate immune system. (harvest-re.com)
  • In this study, we thus investigated the expression of infiltrating lymphocyte subsets (CD3, CD20, CD4, and CD8) and T helper (Th) cytokines including interferon (IFN)-γ (Th1 type), interleukin (IL)-4 (Th2 type), IL-17 (Th17 type), and IL-10 (regulatory T cell type) by immunohistochemistry in buccal mucosa specimens from 2 GS patients compared with 15 OLP patients. (nih.gov)
  • IFN-γ and IL-10 were strongly detected in the infiltrating lymphocytes of all patients. (nih.gov)
  • Lymphocyte subsets in the buccal mucosa from patients with GS and OLP. (nih.gov)
  • Since the block of lymphocyte egress is not complete, we analyzed subset composition and migratory responsiveness towards S1P of the lymphocytes remaining in the circulation of MS patients treated with fingolimod or IFN-β. (biomedcentral.com)
  • Here we show that fingolimod treatment increased the responsiveness of the remaining T-lymphocytes towards S1P and that B cell migration of IFN-β treated patients changed from weak to normal. (biomedcentral.com)
  • Altered cellular immune function has been demonstrated in patients with lung cancer, including decreased numbers of circulating lymphocytes and changes in the percentage of lymphocytes in various functional subsets. (elsevier.com)
  • We quantitated lymphocyte subsets in 54 patients with lung cancer including patients with limited (stages 1 and 2) nonsmall cell lung cancer (NSCLC, n = 23), advanced (stage 3) NSCLC (n = 16), and small cell cancer (SCLC, n = 15). (elsevier.com)
  • Serum albumin was decreased in 15 lung cancer patients, and lymphocyte subsets were separately evaluated in these patients. (elsevier.com)
  • Lymphocyte populations in cancer patients were compared to those of nonsmokers and a smoking patient population. (elsevier.com)
  • A decrease in population of T-lymphocytes subsets is frequent in patients with SCLC, advanced NSCLC, and lung cancer patients with hypoalbuminemia. (elsevier.com)
  • Herein, we investigated lymphocyte subsets in patients with two major subtypes of GBS (acute inflammatory demyelinating polyneuropathy, AIDP, and acute motor axonal neuropathy, AMAN) before and after treatment with IVIG, and explored the possible mechanism of IVIG action. (biomedcentral.com)
  • For patients with PTLD refractory to Rituximab, donor lymphocyte infusion (DLI) is established as a successful option for salvage therapy. (frontiersin.org)
  • We analyzed by flow cytometry the different lymphocytes subsets in the peripheral blood of patients affected by IgM-related neuropathy, chronic inflammatory demyelinating polyneuropathy (CIDP), monoclonal gammopathy of undetermined significance and healthy subjects, to investigate whether different immunological patterns may differentiate the classical phenotype from atypical forms. (unicatt.it)
  • IFN-gamma producing CD4+ and CD8+ T lymphocytes, as well as CD4+ and CD8+ T cells expressing T-bet (T-helper type 1, Th1) were increased in CIDP patients. (unicatt.it)
  • We did not find any significant differences in the different lymphocytes subsets in the IgM-related neuropathy between patients with classical and atypical phenotype. (unicatt.it)
  • T-cell subsets were measured in the peripheral blood of 33 patients with heart failure from idiopathic dilated cardiomyopathy, 22 patients with heart failure from other causes, and 33 normal controls. (elsevier.com)
  • We performed network meta-analyses to determine differences in blood cells, lymphocyte subsets, and cytokines in COVID-19 patients with different clinical stages. (biomedcentral.com)
  • Seventy-one studies were included involving 8647 COVID-19 patients, White blood cell (WBC), neutrophil (NEUT), IL-6, and IL-10 counts increased significantly with worsening of the COVID-19, while lymphocyte (LYM) counts decreased. (biomedcentral.com)
  • By retrospectively reviewing of patients in four groups (healthy controls, severe influenza A, non-severe COVID-19 and severe COVID-19) who were admitted to Ditan hospital between 2018 to 2020, we performed flow cytometric analysis and compared the absolute counts of leukocytes, lymphocytes, and lymphocyte subsets of the patients at different time points (weeks 1- 4). (researchsquare.com)
  • Our study suggests the plausible role of lymphocyte subsets in disease progression, which in turn affects prognosis and recovery duration in patients with severe COVID-19 and influenza A. (researchsquare.com)
  • This study was performed to define the alteration of peripheral blood T lymphocyte subsets in a group of patients with sudden deafness. (kjorl.org)
  • Influence of fingolimod on basic lymphocyte subsets frequencies in the peripheral blood of multiple sclerosis patients - preliminary study. (blogspot.com)
  • Fingolimod is a drug administered orally to adult patients treated for relapsing remitting course of multiple sclerosis (MS). Mode of action of fingolimod is based on intense S1P1 receptor stimulation and "arresting" lymphocytes in lymphatic organs. (blogspot.com)
  • Objective of the research was to assess changes in the frequencies of basic lymphocyte subsets in patients treated for multiple sclerosis with the use of fingolimod. (blogspot.com)
  • Lymphocyte subsets in patients with lung cancer treated with thiophosphoric acid alkaloid derivatives from Chelidonium majus L. (Ukrain). (medicdebate.org)
  • When compared with normal subjects, absolute numbers of T lymphocytes were reduced in patients with active ulcerative colitis or Crohn's disease (p less than 0.05). (ox.ac.uk)
  • Sixteen biopsies from patients with oral lichen planus (10), simple keratosis (3) and lichenoid reactions (3) were studied using monoclonal antibodies directed against lymphocyte markers. (semanticscholar.org)
  • Changes in lymphocyte subsets and cytokines during European porcine reproductive and respiratory syndrome: increased expression of IL-12 and IL-10 and proliferation of CD4(-)CD8(high). (surrey.ac.uk)
  • Cytokines are small protein molecules such as IL-6, IL-10, and TNF-α secreted by T lymphocytes which play a great role in intercellular communications and immunomodulation function [ 9 ]. (biomedcentral.com)
  • In response to cytokine signalling and other factors, CD4-positive T lymphocytes differentiate into distinct populations that are characterized by the production of certain cytokines and are controlled by different master transcription factors. (inat.ru)
  • 1. CD4 counts were linearly related to total lymphocyte concentrations (Pearson correlation coefficient = 0.948), but no relationship was found between total lymphocyte or lymphocyte subset counts and APACHE II score, predicted mortality rate, or survival rate. (nih.gov)
  • The quantity of factor VIII concentrate given to boys with severe haemophilia A (mean U/year) showed a significant inverse correlation with total white cell counts, lymphocyte counts, platelet counts, and the ratio of monoclonal antibody defined T lymphocyte subsets, T4 and T8 (T4:T8). (bmj.com)
  • Treatment with high dose factor VIII concentrate (more than 25 000 U/year) was associated with low platelet counts, low lymphocyte counts, low T4:T8 ratios, and hypergammaglobulinaemia. (bmj.com)
  • The most commonly followed lymphocyte subsets include CD4 and CD8 cell counts and percentages. (thebody.com)
  • Relative counts of peripheral blood T and B lymphocyte subsets were detected by flow cytometry analysis. (biomedcentral.com)
  • CD4+ lymphocyte percentages and absolute counts may be used as aid in evaluation of immune competency. (thumbaylabs.com)
  • Following fingolimod discontinuation lymphocyte counts return to be within the normal range within 6-8 weeks and are above 80% of baseline values by 3 months. (blogspot.com)
  • Therefore low lymphocyte counts in fingolimod-treated MSers does not have the same biological implications as low lymphocyte counts in other situations. (blogspot.com)
  • Importantly, the effectiveness of fingolimod and adverse events is not related to the peripheral lymphocyte counts. (blogspot.com)
  • In contrast the European Medicine Agency (EMA) has recommended the need to check peripheral lymphocyte counts before initiating treatment and have recommended periodic testing at months 3 and then at least yearly after that. (blogspot.com)
  • Until we get more clarity on this we have recommended more frequent blood count monitoring in MSers with lymphocyte counts less than 200, i.e. 3 monthly. (blogspot.com)
  • To study the effect of glutamine supplementation on lymphocyte subpopulation counts in children with acute diarrhea, children aged 6-24 months were enrolled in a double-blind randomized study. (hacettepe.edu.tr)
  • The absolute counts of lymphocyte subsets are known to be influenced by a variety of biological factors, including hormones, the environment, and temperature. (testcatalog.org)
  • The studies on diurnal (circadian) variation in lymphocyte counts have demonstrated progressive increase in CD4 T-cell count throughout the day, while CD8 T cells and CD19+ B cells increase between 8:30 a.m. and noon with no change between noon and afternoon. (testcatalog.org)
  • abstract = "Lymphocyte subsets using monoclonal antibodies have hitherto not been reported in normal Nigerian subjects. (elsevier.com)
  • Normal lymphocyte subsets values among children from Cameroon differ from reported values in Caucasian and some African populations. (nih.gov)
  • Nevertheless, B lymphocytes are one of the most prevalent leukocyte populations in the naive murine heart. (jci.org)
  • As a result, immunophenotyping using multi-color flow cytometry has become one of the most powerful techniques for profiling immune cell subsets within complex populations such as peripheral blood and tissue/tumor samples. (fcslaboratory.com)
  • This study used a lysed whole blood method for analysis of lymphocyte sub-populations in samples from children born and living in two districts: a highly-contaminated area (Michalovce) and one (Svidnik/Stropkov) with ≈≈ 2-fold lower environmental PCB levels. (elsevier.com)
  • There are various subsets of each of these individual populations with specific cell-surface markers and function. (testcatalog.org)
  • The spectrum of such populations, which was initially limited to Th1 and Th2 subsets, is currently broadened to include Th17 and Treg subsets, as well as a number of less studied subtypes, such as Tfh, Th9, and Th22. (inat.ru)
  • We also found lower percentage activated subsets T cells with phenotype CD3+HLA-DR before (x = 4.78) and after treatment (x = 4.03) compared with control (x = 7.27). (prohealth.com)
  • While T cells activated in hypoxia divided less than those activated in atmospheric oxygen, Glut1-Hi lymphocytes exhibited increased effector phenotype acquisition, augmented proliferation, and an inverted CD4/CD8 ratio in both oxygen conditions. (cnrs.fr)
  • As a result, lymphocyte immune phenotype analysis can be used as an important reference index for evaluating the body's immune status. (biomedcentral.com)
  • Furthermore, we assessed the susceptibility and permissiveness of various functionally distinct T and B cells, such as helper T (T H ) cell subsets and IgG- and IgA-positive memory B cells, in peripheral blood and tonsils. (asm.org)
  • T-lymphocyte and helper/suppressor cell immune status assist in evaluating cellular immunocompetency. (specialtylabs.com)
  • Greater numbers of helper T lymphocytes (Leu-3+) were found at the periphery than in the central portion of the same granuloma. (semanticscholar.org)
  • Counting the number of helper T-cells -- technically called CD4+ lymphocytes -- is perhaps the most important tool used to assess the overall health of the immune system in people with HIV. (thebody.com)
  • T Helper Lymphocyte Subsets and Plasticity in Autoimmunity and Cancer: An Overview. (inat.ru)
  • This study aimed to analyze the role of lymphocyte subsets in the immunopathogenesisof COVID-19 and severe influenza A, and examined the clinical significance of their alterations in the prognosis and recovery duration. (researchsquare.com)
  • Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, and profiled dynamic expression of markers during thymocyte differentiation, T-cell maturation, and between functionally distinct B-cell subset clusters. (frontiersin.org)
  • Transmigration of lymphocyte subsets across these barriers and their activation/differentiation within the periphery and intrathecal compartments in health and autoinflammatory CNS disease are complex. (mdpi.com)
  • Mathematical models are warranted that qualitatively and quantitatively predict the distribution and differentiation stages of lymphocyte subsets in the blood and CSF. (mdpi.com)
  • Observation of percentages changes of lymphocytes (CD19), T(CD3), subsets CD4, CD8, NK cells, activated lymphocytes with fenotype CD3+HLA-DR, CD4/CD8 rate in Lyme borreliosis was carried out before (I examination) and after antibioticotherapy (II examination). (prohealth.com)
  • CD3, CD4 and CD8 percentages are reported as a percent of total lymphocytes. (aruplab.com)
  • In addition, the sum of the percentages of BB3+ and delta-TCS-1+ cells approximated the percentages of peripheral blood CD3+WT31- lymphocytes in seven normal donors tested. (rupress.org)
  • In addition, the sum of the percentages of BB3 + and δ-TCS-1 + cells approximated the percentages of peripheral blood CD3 + WT31 - lymphocytes in seven normal donors tested. (elsevier.com)
  • Stable levels of CD8+ effector cells were observed together with a lack of association of CD8+ lymphocytes with graft function, such as GFR and serum creatinine. (harvest-re.com)
  • This reagent is for research purposes only: this kit is used to determine the content of T-Subset in human serum, plasma, tissue fluid and related fluid samples. (toyscalm.com)
  • The resulting dataset included median antibody binding capacities (ABCs) and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. (frontiersin.org)
  • Percentage of lymphocytes measured in area D1+D2 of the XN WDF and W1/W2 ratio from the WPC channel. (clinicaltrials.gov)
  • Changes of immune function relevant inflammatory factor of IL-6, TNF-α concentration, regulatory T cell (Tregs), T-lymphocyte subsets, CD4+T cell, CD8+T cell, Natural Killer cell (NK) percentage in vivo before and after treatment in two groups were detected and analysed. (alliedacademies.org)
  • However, the identification of immune cells such as macrophages and T lymphocytes in atherosclerotic plaques sparked the interest of researchers in understanding the precise roles of these cells in atherosclerosis. (springer.com)
  • Oral lichen planus (OLP) is a chronic inflammatory disease of the oral mucosa characterized by destruction of basal cells by Langerhans cells, macrophages, and T lymphocytes. (nih.gov)
  • Specifically, NKG2D is expressed on the surface of most NK cells, activated CD8 + αβ T cells, antigen-specific CD8 + memory T cells, a subset of γδ T cells and on activated macrophages ( 10 , 17 ). (aacrjournals.org)
  • Macrophages are directly involved in the fight against invading micro-organisms as well as being antigen-presenting cells which activate lymphocytes. (wikipedia.org)
  • Moroccan lymphocyte subsets reference ranges: age, gender, ethnicity, and socio-economic factors dependent differences. (medworm.com)
  • This study provides reference ranges for lymphocyte subsets of healthy Moroccan adults. (medworm.com)
  • It substantially duplicated a previous publication ("Lymphocyte Subset Reference Ranges in Healthy Saudi Arabian Children," by S. Shahabuddin, I. H. Al-Ayed, M. O. Gad El-Rab, and M. I. Qureshi, Pediatr. (asm.org)
  • Reference ranges for lymphocyte subsets in healthy adult male Omanis. (elsevier.com)
  • OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. (elsevier.com)
  • Lymphocyte subset reference ranges in adult Caucasians. (ox.ac.uk)
  • It appears possible to recommend reference ranges for lymphocyte population parameters applicable across national and laboratory boundaries. (ox.ac.uk)
  • Abnormalities in the proportions of immunoregulatory T-lymphocytes in bronchoalveolar lavage fluid have been reported in interstitial pulmonary diseases, yet the effect of cigarette smoking on T-cell subsets in bronchoalveolar lavage fluid from normal subjects has not been investigated. (nih.gov)
  • We applied an immunoperoxidase technique performed on glass slides using the monoclonal antibodies, OKT3, OKT4, OKT8, OKIa, and Leu-7 to study T-cell subsets in bronchoalveolar lavage fluid and blood of 11 normal nonsmokers and 12 smokers. (nih.gov)
  • These abnormalities of lymphocyte subsets in bronchoalveolar lavage fluid correlated with the cumulative pack-years of smoking history but were not reflected in the peripheral blood. (nih.gov)
  • We determined the effects of gender, race, and age on the following lymphocyte subset markers: total CD4 + cells, CD4 + naïve cells, CD4 + memory cells, all CD8 + cells, CD8 + naïve cells, CD8 + memory cells, CD16 + natural killer cells, and CD19 + B cells. (asm.org)
  • This particular Treg subset co\expresses the activation marker HLA\DR and appears to impact effector cells functionally by release of TGF\ or via CTLA\4\mediated cell conversation with DC in lymph nodes. (harvest-re.com)
  • Lymphocytes strongly contribute to the pathology of multiple sclerosis (MS). Thereby, frequently activated or reactivated autoreactive lymphocytes differentiate into effector cells that enter the central nervous system (CNS) causing tissue inflammation and damage. (biomedcentral.com)
  • The immunomodulatory drug fingolimod (FTY720, Gilenya) directly targets S1P receptors and inhibits lymphocyte egress, whereas the exact effector function of interferon-β (IFN-β) in MS therapy remains elusive. (biomedcentral.com)
  • Flow cytometry analysis of lymphocyte subset markers was performed for a group of sexually active, human immunodeficiency virus (HIV)-negative adolescents over a 2-year period to establish normative data. (asm.org)
  • Since similar molecular patterns were consistently detected among all BB3 + as well as among all δ-TCS-1 + clones analyzed, we conclude that BB3 and δ-TCS-1 mAbs identify two distinct molecular forms of TCR-γ/δ expressed by two different peripheral blood T cell subsets. (elsevier.com)
  • LHTL could affect T lymphocyte subsets significantly as a result of the two simultaneous stimuli of physical activity and exposure to hypoxia. (bmj.com)
  • Notably, Glut1 upregulation in response to TCR stimulation was significantly higher in T lymphocytes activated under hypoxic as compared to atmospheric oxygen conditions. (cnrs.fr)
  • Moreover, Glut1-Hi T lymphocytes exhibited a significantly enhanced ability to produce IFN-gamma and this secretion potential was completely dependent on continued glycolysis. (cnrs.fr)
  • Compared with the less-contaminated region, Michalovce samples showed significantly higher expression of CD3 + T-lymphocytes, B-lymphocytes, and activated B-lymphocytes, whereas NK cells were less expressed. (elsevier.com)
  • Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patien. (biomedsearch.com)
  • Lymphocyte subsets, phytohaemagglutinin responsiveness of blood lymphocytes, and interleukin 2 production in sarcoidosis. (bmj.com)
  • It would appear that the OKT4+ cells are largely responsible for the time of day variation in peripheral blood lymphocytes, owing probably to a greater sensitivity to changes in plasma cortisol levels. (elsevier.com)
  • Here, we present the results of the first phase of the CD Maps study, mapping the expression of CD1-CD100 ( n = 110) on 47 immune cell subsets from blood, thymus, and tonsil using an eight-color standardized EuroFlow approach and quantification of expression. (frontiersin.org)
  • At Flow Contact Site Laboratory, LLC we offer a variety of panels for immunophenotyping of lymphocyte subsets and other immune cell subsets for both preclinical and clinical studies. (fcslaboratory.com)
  • Immunophenotyping is a technique to analyze the immune cell subsets by detecting proteins expressed on the surface or within the cell (intracellular) using antigen specific antibodies. (fcslaboratory.com)
  • Drawing a on the wealth of knowledge found in the literature, combinations of antigen-specific antibodies which are tagged with different fluorochromes can be put together to design antibody panels for the identification of specific immune cell subsets. (fcslaboratory.com)
  • Significant information can be gained by identifying these cellular subsets and monitoring the impact of a given treatment on the distribution of these immune cell subsets. (fcslaboratory.com)
  • Lymphocyte subsets are one important clinical test that can reveal the state of cellular immune and humoral immune. (chinesemedicinekidney.com)
  • However, although in vivo lymphocyte expansion has been correlated with good clinical outcome following DLI, the specificity and functional characteristics of EBV-specific T-cell responses remain poorly characterized. (frontiersin.org)
  • Further studies involving a larger patient population are needed to evaluate if different lymphocytes subset may be involved in different clinical phenotypes of IgM-related neuropathy. (unicatt.it)
  • The aim of this study was to investigate the clinical usefulness of BAL cellular analysis with lymphocyte subsets for the differential diagnosis of DILDs. (bvsalud.org)
  • In the clinical studies ~20% of study subjects had a least one lymphocyte count below this level. (blogspot.com)
  • Our findings suggested that abnormalities of peripheral lymphocyte subsets were related to the immune disorder of lupus itself, regardless of immunosuppressive treatment. (scielo.br)
  • Sphingosine-1-phosphate (S1P) is a key mediator of lymphocyte egress that binds to five S1P receptors (S1P1-5). (biomedcentral.com)
  • Lymphocytes and their subsets, NK cells were signed and measured by flow cytometric immunophenotyping in Coulter EPIX XL with Becton-Dickinson antibodies twice: before and after treatment. (prohealth.com)
  • Some examples of our immunophenotyping of specific lymphocyte subsets in various species are shown below. (fcslaboratory.com)
  • Lymphocyte immunophenotyping is an important tool in the diagnosis of immunologic and hematologic disorders. (elsevier.com)
  • CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. (elsevier.com)
  • CD4 and CD8 T lymphocyte activation requires the generation of sufficient energy to support new biosynthetic demands. (cnrs.fr)
  • Increased numbers of central memory CD8 + T lymphocytes with increased pro-inflammatory activation are found in the blood of CRPS participants compared to healthy controls. (biomedcentral.com)
  • Cantor H and Boyse EA (1975) Functional subclasses of T‐lymphocytes bearing different Ly antigens. (els.net)
  • The lymphocyte subpopulation distributions for all antigens were found to be similar at all sites. (ox.ac.uk)
  • Initial models of highly stable T‐cell subsets were useful but underestimated diversity. (els.net)
  • Correlations between T-lymphocyte subset values and stages or cell types of disease were sought. (biomedsearch.com)
  • In a cohort of 352 healthy children, aged 0 to 6 years, the relative and absolute numbers of T-cell subsets, B cells, and NK lymphocytes were determined from peripheral blood collected in EDTA tubes. (nih.gov)
  • Current methods of polychromatic flow cytometry and PCR-based detection of k deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. (muni.cz)
  • In regards to lymphocytes in the peripheral blood, the machine has the capacity to distinguish activated from non-activated T-cell subsets using a very small volume of EDTA sample (88uL) (including remnant sample from a standard full blood count) with results available in 1.5 minutes. (clinicaltrials.gov)
  • Within the splenic and lymph node CD4+ T cell compartment, Ly-6C expression is restricted to Ly-6.2 strains of mice, and is present on a subset of naive cells. (jimmunol.org)
  • Alterations in immunoregulatory T-cell subsets in cigarette smokers. (nih.gov)
  • However, information on the relative susceptibility of B-cell subsets is scarce. (asm.org)
  • Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is a rare B-cell lymphoma considered to be of germinal center (GC) derivation. (ovid.com)
  • Thus, different patterns of T-cell subset changes were demonstrated between CB MNCs and APB MNCs in response to IL-12 and/or IL-15. (eurekamag.com)
  • This study aimed to examine the relationship between altered immune status and HCV-related liver disease, by determining the changes in peripheral blood lymphocyte and natural killer (NK) cell subsets in these subjects. (hku.hk)
  • To explore influences of lenalidomide on multiple myeloma, inflammatory factor, regulatory T cell and T-lymphocyte subsets. (alliedacademies.org)
  • The costimulatory effect is greater on the CD8+ than the CD4+ T cell subset. (rupress.org)
  • Therefore, human T cell costimulation can be achieved pharmacologically with thalidomide, and preferentially in the CD8+ T cell subset. (rupress.org)
  • Our instrumentation allows up to 10 parameters to be measured in a single tube and our analysis software utilizes complex gating strategies for narrowing specific lymphocyte subsets from the larger pool of cell types present in the sample. (fcslaboratory.com)
  • Lymphocyte function is related to the numerous complex superficial cell membrane proteins on the cell surface. (biomedcentral.com)
  • Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) is a life-threatening complication of T-lymphocyte deplete allogeneic hematopoietic stem cell transplantation (allo-HSCT). (frontiersin.org)
  • The use of magnetized cells holds promise for further applications in automated genotoxic and immunological cell assays of mononuclear leukocyte subsets. (aaccjnls.org)
  • Each of these lymphocyte subsets can be identified by a combination of one or more cell surface markers. (testcatalog.org)
  • T-lymphocytes (T-cells) are white blood cells which take part in cell-mediated immunity. (wikipedia.org)
  • T-lymphocyte subsets in acute illness. (nih.gov)
  • Cross-sectional study, comparing Acute Physiology and Chronic Health Evaluation II (APACHE II) scores and the calculated, disease-specific, predicted mortality rate with T-lymphocyte subsets. (nih.gov)
  • Study question: Do participants who have chronic or acute viral infections i.e. untreated HIV, HIV-HCV with treated HIV and untreated HCV, untreated HCV monoinfection or untreated HBV monoinfection, have a significant excess of activated lymphocytes as measured by D1+D2 on the XN WDF channel using the XN-20 analyser? (clinicaltrials.gov)
  • Sequentially CD4+ T lymphocytes increased gradually in number from initial skin lesions and were major composition cells in the tumor lesions. (koreascience.or.kr)
  • This is particularly relevant to fingolimod, which is a non-depleting agent and traps lymphocytes in the peripheral lymph nodes. (blogspot.com)
  • Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. (elsevier.com)
  • Gender was the demographic characteristic most frequently associated with differences in lymphocyte subset measures. (asm.org)
  • No major differences were seen between groups for IgG levels and lymphocyte subsets. (iospress.com)
  • No significant differences were found between patient and control groups in the proportions of circulating T lymphocytes or their subsets. (ox.ac.uk)
  • There were no differences in T lymphocyte numbers between the patient groups and the disease control subjects. (ox.ac.uk)
  • Age and sex associated differences are substantial for some lymphocyte subsets (CD3, CD4, NK cells), and proper accounting of these effects is essential in evaluating the individual patient, if further disease-related variation is to be accurately and consistently assessed. (ox.ac.uk)
  • These molecules are essential markers for the identification and isolation of leukocytes and lymphocyte subsets. (frontiersin.org)
  • No statistical changes in subsets with receptor for IL-2 before and after treatment was shown. (prohealth.com)
  • T lymphocyte subsets in inflammatory bowel disease: peripheral blood. (ox.ac.uk)
  • The study results showed a great advantage to using the Aquios CL for automated lymphocyte subset enumeration. (labroots.com)
  • This assay is CD4+ lymphocyte subset enumeration using Pan Leukocyte (CD45+) Gating strategy. (thumbaylabs.com)
  • Distribution of Ly-6C on lymphocyte subsets: I. Influence of allotype on T lymphocyte expression. (jimmunol.org)
  • The expression patterns of the Ly-6C Ag were examined on splenic and thymic lymphocyte subsets of Ly-6.1 and Ly-6.2 strains of mice using the rat mAb 15.1. (jimmunol.org)
  • Some parameters that were out of favorable limits (elevated expression of CD56, CD158a in T lymphocytes, decreased levels of CD4 T lymphocytes, up-regulated expression of HLA DR in CD8+ T cells and NK cells, elevated number of NK cells and increased NK cytotoxicity, increased and decreased expression of CD158a and CD8 in NK cells) were considered in our study to be immune deviations (ID) potentially predictive for IVF failure [17]. (termedia.pl)
  • However, IFN-β is known to induce CD69 expression, which by direct interaction, downmodulates S1P1 surface expression preventing lymphocyte egress. (biomedcentral.com)
  • b Intracellular signalling and functional marker expression levels in the FlowSoM cluster representing central memory CD8 + T lymphocytes. (biomedcentral.com)
  • We can evaluate specific lymphocyte subsets in human, rodent (rat/mice), NHP and dog specimens. (fcslaboratory.com)
  • T-lymphocyte subsets (T-Subset) were added to the monoclonal antibody-coated wells in turn, followed by The HRP-labeled T lymphocyte subset (T-Subset) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. (toyscalm.com)