Azure Stains
Methylene Blue
A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.
Staining and Labeling
Serratia liquefaciens
Serratia
Industrial Waste
Environmental Remediation
Lignin
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Fabry Disease
An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.
alpha-Galactosidase
Enzyme Replacement Therapy
Microvessels
Trihexosylceramides
Glycosphingolipids which contain as their polar head group a trisaccharide (galactose-galactose-glucose) moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in ceramide trihexosidase, is the cause of angiokeratoma corporis diffusum (FABRY DISEASE).
Bone Morphogenetic Proteins
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 7
Bone and Bones
Bone Morphogenetic Protein Receptors, Type I
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Microscopy, Electron, Scanning Transmission
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Biological Specimen Banks
Image Processing, Computer-Assisted
Libraries
Internet
Models, Biological
Health Education
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Gene Library
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Pulmonary Veins
Myocytes, Cardiac
Microscopy, Electron, Transmission
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Myocardium
Cells, Cultured
Leukocyte Count
Eosine Yellowish-(YS)
Bone Marrow
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
AMP-Activated Protein Kinases
Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP.
Protein Subunits
Amino Acid Sequence
IMP Dehydrogenase
Guanosine Monophosphate
Structure and inheritance of some heterozygous Robertsonian translocation in man. (1/163)
Banding studies in 25 Robertsonian translocations showed that all could be interpreted as stable dicentrics. The mechanism for their stability is likely to be the proximity of their centromeres but centromeric suppression could also have a role. In many of these dicentric translocations, discontinuous centromeric suppression, as indicated by chromatid separation at one of the centromeric regions, was observed in C-banded preparations. A further observation of undefined relation to the first was that the ratio of the two constitutive centromeric heterochromatin (CCH) regions from the component chromosomes of the translocations was variable in the same translocation type, e.g. t(13;14). It is proposed that this ratio may influence the segregation ratio. Abnormal spermatogenesis is suggested as the likely mechanism for the difference in the proportion of aneuploid offspring in the progeny of maternal and paternal heterozygotes. Neither of the t dic(21;21)s could be interpreted as isochromosomes. It is proposed that Robertsonian fusion translocations be defined as stable, dicentric, whole-arm translocations, with both centromeres in a median position and resulting in the loss of a small acentric fragment during this formation. It is suggested that they occur at high frequency between telocentric or, as in man, certain acrocentric chromosomes because of some intrinsic property of those chromosomes not possessed by metacentric chromosomes and mediated by interphase association of centromeres. (+info)Comparison of five methods of malaria detection in the outpatient setting. (2/163)
In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative. (+info)Detection of "Candidatus Helicobacter suis" in gastric samples of pigs by PCR: comparison with other invasive diagnostic techniques. (3/163)
Recently, a new 16S ribosomal DNA-based PCR assay was developed for the specific detection of "Candidatus Helicobacter suis" (former "Gastrospirillum suis") in porcine gastric samples. In the present study, this PCR assay was compared to three other invasive diagnostic methods (rapid urease test, immunohistochemistry, histologic analysis by Giemsa staining). Antral stomach samples from 200 slaughterhouse pigs from Belgium and The Netherlands were examined. Bacterial presence was determined in 77% (154 of 200) of the samples by PCR in combination with Southern blot hybridization, 56% (111 of 200) of the samples by immunohistochemistry, 61% (122 of 200) of the samples by urease testing (20 h postinoculation [p.i.]), 36% (71 of 200) of the samples by urease testing (3 h p.i.), and 33% (65 of 200) of the samples by Giemsa staining. The intrinsic specificity of the PCR assay was assessed by Southern blot analysis with an "Candidatus H. suis"-specific probe and sequencing of PCR products. Interassay sensitivity and specificity values were assessed for each test by pairwise comparisons between tests. Agreement between tests was evaluated by calculating Cohen's kappa coefficient. From that analysis, the PCR assay was considered the most reliable benchmark. Microscopic detection of immunohistochemically labeled or Giemsa-stained "Candidatus H. suis" cells in stomach sections proved to be highly specific (100%) but relatively insensitive (72 and 42%, respectively) compared to the PCR assay. A longer incubation time of the urease test improved its sensitivity considerably (74 versus 55%) but was accompanied by a loss of specificity (72 versus 93%). In conclusion, we found the "Candidatus H. suis"-specific PCR assay to be a sensitive and reliable diagnostic method for the detection of "Candidatus H. suis" in the stomachs of pigs and could prove to be a valuable tool for further epidemiological studies both for "Candidatus H. suis"- and for "Helicobacter heilmannii" type 1-related research. (+info)Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability. (4/163)
This study was designed to assess whether nucleic acid stains such as propidium iodide and Hoechst 33258 and the cytosolic stain eosin identified equivalent proportions of non-viable cells. Sub-samples of boar spermatozoa stored for up to 72 h, and frozen bull spermatozoa stored in straws and thawed before staining, were exposed to either propidium iodide or Hoechst 33258 alone or in combination. Additional sub-samples were stained with eosin-nigrosin and subsequently with Giemsa. The proportion of non-viable cells identified by propidium iodide alone was equivalent to that observed when it was used in combination with the other fluorescent probe. Similar results were observed for Hoechst 33258. However, direct microscopic examination of sub-samples exposed to both stains revealed that a proportion of spermatozoa stained with propidium iodide did not incorporate Hoechst 33258. This was found consistently in boar and bull spermatozoa under the different experimental conditions used. Quantification showed that the proportion of propidium iodide-positive cells was significantly higher than Hoechst 33258-positive cells. Furthermore, the proportion of propidium iodide-positive cells was higher than cells stained with eosin, but no differences were found between the number of cells stained with Hoechst 33258 or eosin. The proportion of cells stained with propidium iodide was positively correlated with the proportion stained with either Hoechst 33258 or eosin, despite the observation that more cells incorporated propidium iodide. Taken together, these results indicate that there are differences in the ability of fluorescent probes to identify non-viable sperm cells and that this should be considered when staining protocols are used to analyse sperm viability, or when viability is used as a discriminating factor in functional studies, such as those related to acrosomal exocytosis. (+info)Histological identification of Helicobacter pylori: comparison of staining methods. (5/163)
AIM: To determine whether two recently described staining methods (the modified McMullen's and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. METHODS: Histological sections from 63 paired gastric biopsies from adult patients previously investigated for dyspepsia were stained with the four methods and these were assessed blindly and independently by two observers. Of the 63 patients, 30 were originally negative in all tests for H pylori infection, 30 were positive, and the remaining three cases had discordant results using a combination of five tests (rapid biopsy urease test, urea breath test, culture, serology, and histology). RESULTS: Interobserver agreement was best with the antibody method (98%), followed by the McMullen's (90%), Giemsa (87%), and HpSS (85%). Of the 60 "gold standard" positive and negative cases, 30 were positive by the modified Giemsa stain, 29 by the McMullen's method, 29 by HpSS, and 30 by the antibody stain. However, there were two false positives with the HpSS method. The modified Giemsa is the cheapest and easiest to perform technically. CONCLUSIONS: When H pylori are present, careful examination will almost always reveal them, whichever of these stains is used. However, the modified Giemsa stain is the method of choice because it is sensitive, cheap, easy to perform, and reproducible. (+info)Morphologic aspects of Tetratrichomonas didelphidis isolated from opossums Didelphis marsupialis and Lutreolina crassicaudata. (6/163)
Tetratrichomonas didelphidis (Hegner & Ratcliffe, 1927) Andersen & Reilly, 1965 is a flagellate protozoan found in the intestine, cecum, and colon of Didelphis marsupialis. The parasitic protozoa used in this study was found and isolated in the intestine of opossums in Pavlova starch-containing medium in Florianopolis, State of Santa Catarina, Brazil, from D. marsupialis and Lutreolina crassicaudata. The strains were cultivated in Diamond medium without maltose and with starch solution, pH 7.5 at 28 degrees C. The specimens were stained by the Giemsa method and Heidenhain's iron hematoxylin. The light microscopy study of the trophozoites revealed the same morphologic characteristics as specimens previously described. (+info)Atypical Herpes simplex keratitis (HSK) presenting as a perforated corneal ulcer with a large infiltrate in a contact lens wearer: multinucleated giant cells in the Giemsa smear offered a clue to the diagnosis. (7/163)
PURPOSE: To report a case of atypical herpes simplex keratitis initially diagnosed as bacterial keratitis, in a contact lens wearer. RESULTS: Case report of an 18-year-old woman using contact lenses who presented with pain, redness and gradual decrease in vision in the right eye. Examination revealed a paracentral large stromal infiltrate with a central 2-mm perforation. Corneal and conjunctival scrapings were collected for microbiological investigations. Corneal tissue was obtained following penetrating keratoplasty. Corneal scraping revealed no microorganisms. Giemsa stained smear showed multinucleated giant cells. Conjunctival, corneal scrapings and tissue were positive for herpes simplex virus - 1 (HSV) antigen. Corneal tissue was positive for HSV DNA by PCR. CONCLUSIONS: Atypical HSV keratitis can occur in contact lens wearers. A simple investigation like Giemsa stain may offer a clue to the diagnosis. (+info)Newly recognized cellular abnormalities in the gray platelet syndrome. (8/163)
The gray platelet syndrome (GPS) is a rare congenital bleeding disorder in which thrombocytopenia is associated with increased platelet size and decreased alpha-granule content. This report describes 3 new pediatric cases presenting with the classical platelet abnormalities of GPS within one family with normal parents. Examination of blood smears of the 3 patients demonstrated not only gray platelets, but also gray polymorphonuclear neutrophils (PMNs) with decreased or abnormally distributed components of secretory compartments (alkaline phosphatase, CD35, CD11b/CD18). Secondary granules were also decreased in number as assayed by immunoelectron microscopy. These data confirm that the secretory compartments in neutrophils were also deficient in this family. Megakaryocytes (MKs) were cultured from the peripheral blood CD34+ cells of the 3 patients for 14 days, in the presence of thrombopoietin and processed for immunoelectron microscopy. Although von Willebrand factor (vWF) was virtually undetectable in platelets, vWF immunolabeling was conspicuous in cultured maturing MKs, particularly within Golgi saccules, but instead of being packaged in alpha-granules, it was released into the demarcation membrane system. In contrast, P-selectin followed a more classical pathway. Double-labeling experiments confirmed that vWF was following an intracellular pathway distinct from the one of P-selectin. In these 3 new cases of GPS, the MKs appeared to abnormally process vWF, with secretion into the extracellular space instead of normal alpha-granule packaging. Furthermore, the secretory compartment of another blood cell line, the neutrophil, was also affected in this family of GPS. (+info)
Giemsa stain legal definition of Giemsa stain
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No data available that match "azure stains"
EosinSmearsRomanowskyCytoplasmGranulesChromosomesGiemsa stain usingMethylene blue-azureHistologicalBiologicalFixationReagentsReticulocytesThin blood smearDyes stainHistologyEosinophilsBasic dyesProtocolsHematologyDiluteTechniqueTissue2000SpecimensMixtureGustav GiemsaHematoxylinDifferentiallyShades of bWright StainBacterialAcidicSupravitalDifferential stainSpecimenAbnormalitiesGiemsa'sNuclearNeuronsChromatinCounterstainPositivelyBloodGramSlidesPreparationReticulocyteCutaneousTissuesNeutralAqueousBasophilicMagnificationPreparationsEpithelialProcedurePropidium iodideSquamous
Eosin18
- stain 1 is May-Grünwald: combines acidic eosin and alkaline methylene blue. (horiba.com)
- eosin: stains orange-red the alkaline components of the cell. (horiba.com)
- Wright stain is a polychromatic stain consisting of a mixture of eosin and methylene blue. (horiba.com)
- The eosin ions are negatively charged and stain basic cell components an orange to pink color. (horiba.com)
- Subsequently, the sections were stained with hematoxilin/eosin, elastic van Gieson and Masson`s trichrome according to standard procedures. (medsci.org)
- The most important components of these stains are oxidized methylene blue, azure B and eosin Y dyes. (sigmaaldrich.com)
- The eosin Y dye stains the cytoplasm of cells an orange to pink color. (sigmaaldrich.com)
- Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. (wikipedia.org)
- Biological stains and staining protocols Histology Leishman stain Microscopy Romanowsky stain Wright's stain Giemsa G (1904 Eine Vereinfachung und Vervollkommnung meiner Methylenblau-Eosin-Färbemethode zur Erzielung der Romanowsky-Nocht'schen Chromatinfärbung. (wikipedia.org)
- Eosinophils stain well with eosin! (histosearch.com)
- Romanowsky stains are composed of methylene blue, polychromes (azures) and eosin. (vin.com)
- Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. (reference.com)
- Giemsa stain is a mixture of Azure, Methylene blue, and Eosin dye. (reference.com)
- Azure B is used for preparing azure eosin stains for blood smears. (chemicalbook.com)
- It is used in making azure eosin stains for blood smear staining and is an active metabolite of methylene blue. (chemicalbook.com)
- Romanowsky stains Staining techniques: Romanowsky-type stains are neutral stains composed of a mixture of oxidized methylene blue (azure) dyes and Eosin Y. (vetstream.com)
- Giemsa's staining solution (composed of methylene blue, azure and eosin) is one of the most popular microscopic stains, commonly used in hematology , histology , cytology and bacteriology for in vitro diagnostic (IVD) use. (merckmillipore.com)
- Dissolve 0.76 g Giemsa's azure eosin methylene blue (dry dye) in 50 mL glycerol (85%) and heat for 1 h at 55°C on a water bath, add 50 mL methanol, leave to stand for 24 hours and filter. (merckmillipore.com)
Smears10
- Wright and Giemsa stains are Romanowsky stains used to stain peripheral blood and bone marrow smears. (sigmaaldrich.com)
- Formula: Mixture Formula Weight: N/A CAS Number: Synonym(s): Hematology Stain Description: General Product Use: Polychromic stain for peripheral blood and bone marrow smears. (thomassci.com)
- citation needed] Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens. (wikipedia.org)
- In this presentation, I will review the preparation and staining of blood smears, the components involved in the microscopic evaluation of blood, and provide examples of important abnormal findings. (vin.com)
- Artifacts may result from improper handling and staining of smears. (vin.com)
- Avoid contact of smears and stains with water! (vin.com)
- New methylene blue can be used as a supravital stain to examine reticulocytes, or may be applied to dried smears. (vin.com)
- The following procedures describe staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. (merckmillipore.com)
- Parasitaemias were determined after staining the smears with Giemsa's stain. (elsevier.com)
- Blood samples from the patients who were not smear-negative by day 3 were carefully checked for parasites, by staining smears with Acridine Orange and by a PCR-based assay. (elsevier.com)
Romanowsky4
- Romanowsky stains are used to perform differential white blood cell counts and to study red blood cell morphology. (sigmaaldrich.com)
- Any Romanowsky-type stain is acceptable. (vin.com)
- Giemsa stain is a type of Romanowsky stain, named after Gustav Giemsa, a German chemist who created a dye solution. (reference.com)
- Romanowsky stains also stain granules differentially. (reference.com)
Cytoplasm4
- Synonym: Giemsa Solution MDL number: MFCD00081642 PubChem Substance ID: 24895392 Application When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. (thomassci.com)
- Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta. (wikipedia.org)
- Light Green SF yellowish stains the cytoplasm of all other cells. (ihcworld.com)
- All the vital dyes stain the food vacuoles, and all produce small, darkly stained granules in colourless vacuoles in the cytoplasm. (biologists.org)
Granules11
- acidophil g's granules staining with acid dyes. (thefreedictionary.com)
- amphophil g's granules that stain with both acid and basic dyes. (thefreedictionary.com)
- azurophil g's ( azurophilic g's ) coarse reddish granules that contain myeloperoxidase and stain easily with azure dyes, found in mature neutrophils and their precursor cells. (thefreedictionary.com)
- iodophil g's granules staining brown with iodine, seen in polymorphonuclear leukocytes in various acute infectious diseases. (thefreedictionary.com)
- In certain bacteria, yeasts, yeastlike fungi, and protozoa, metachromatic granules appear red when stained with a blue dye. (thefreedictionary.com)
- Paneth cell granules and tails of spermatozoa also stain but are unlikely to cause confusion. (histosearch.com)
- Acidic components of cells stain deep blue to purple (DNA, RNA, some granules). (vin.com)
- Basic components of cells stain pink (hemoglobin, eosinophil granules). (vin.com)
- Azurophilic" components stain pink-purple (monocyte, platelet, and myeloid precursor granules). (vin.com)
- And "neutrophilic" components stain with both acidic and basic portions of the dye (neutrophil granules). (vin.com)
- A) Kidney biopsy which was obtained prior to agalsidase beta therapy shows dark-staining granules in podocytes. (biomedcentral.com)
Chromosomes5
- Giemsa stain is used in Giemsa banding, commonly called G-banding, to stain chromosomes and often used to create a karyogram (chromosome map). (wikipedia.org)
- Giemsa stain is also used to visualize chromosomes. (wikipedia.org)
- Giemsa stain is also used to visualize chromosomes.This is particularly relevant for detection of Cytomegalovirus infection, where the classical finding would be an 'owl-eye' viral inclusion. (reference.com)
- The genes for major ribosomal RNA were localized on chromosomes 5pter-p15, 9q64-qter, and 13q38-qter of the house musk shrew, Suncus murinus (Insectivora, Soricidae) by silver staining of mitotic metaphase and meiotic pachytene spreads and fluorescence in situ hybridization using the human 28S-RNA genes as a probe to mitotic metaphase spreads. (biomedsearch.com)
- The analysis of chromosomes was performed through Ag-staining, C-banding, chromomycin A3 and DAPI staining, and fluorescent in situ hybridization with ribosomal genes. (biomedsearch.com)
Giemsa stain using1
- The most commonly used Romanowski stains are giemsa stain using giemsa dye, wright stain,may grunwald stain and Jenner's stain but of all these stains,Leishman and Wright are widely used in routine staining. (reference.com)
Methylene blue-azure2
- Tissue fixed for electron microscopy was cut at 0.5 µ, stained with methylene blue-azure II, and quantitatively analyzed. (annals.org)
- 1974 ) A simple methylene blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections. (biologists.org)
Histological2
- The purpose of this study is to evaluate the efficacy and safety of the Matrix RF for Port Wine Stains based on clinical and histological analyses. (bioportfolio.com)
- An approach to the thermodynamics of histological dyeing illustrated by experiments with Azure A. (springer.com)
Biological6
- Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. (wikipedia.org)
- Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. (wikipedia.org)
- Staining is not limited to biological materials, it can also be used to study the structure of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers. (wikipedia.org)
- In vitro staining involves colouring cells or structures that have been removed from their biological context. (wikipedia.org)
- Biological stain. (chemicalbook.com)
- They are used as biological stains. (bvsalud.org)
Fixation3
- Sometimes heat fixation is used to kill, adhere, and alter the specimen so it accepts stains. (wikipedia.org)
- Staining requires fixation, buffering, and staining. (vin.com)
- Wright-Giemsa Stain Method EMS Catalog #: 26149-Series Fixation: Streak thin (approx. (reference.com)
Reagents1
- Quality Control: Always check new batches of stain and reagents for correct staining reactions using a smear containing known Gram-positive and Gram-negative organisms. (reference.com)
Reticulocytes1
- Used for staining reticulocytes. (chemicalbook.com)
Thin blood smear2
- Diluting the Giemsa Stain for Thin Blood smear: For staining the thin blood smear the Giemsa stain is used in 1:20. (reference.com)
- Malaria was diagnosed based on clinical features along with positive Quantitative Buffy Coat method (QBC MP) or thin blood smear examination (Giemsa stain). (elsevier.com)
Dyes stain1
- The methylene blue and azure B dyes stain the nucleus varying shades of blue to purple. (sigmaaldrich.com)
Histology3
- Stains and dyes are frequently used in histology (the study of tissue under the microscope) and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of disease at a microscopic level. (wikipedia.org)
- May be used for progressive or regressive staining in cytology and histology. (thomassci.com)
- Download our scientific poster "Giemsa: The Universal Diagnostic Stain" to view examples of Giemsa's application in hematology, histology, cytology, bacteriology, and cytogenetics. (merckmillipore.com)
Eosinophils2
- If used in an alkaline solution (pH 8 to 9) , eosinophils are the only stained objects in most tissues. (histosearch.com)
- In general, eosinophils stand out in sections stained by any anionic dye that can be used at pH 8-9. (histosearch.com)
Basic dyes4
- 1. a granule that stains with basic dyes. (thefreedictionary.com)
- 1. any granule staining with basic dyes. (thefreedictionary.com)
- All the basic dyes used with the possible exception of azure C, methyl green, and pyronine G attach to the external membrane of A. proteus in an orientated manner, as shown by the increase in birefringence of the external membrane induced by these dyes. (biologists.org)
- The azures are basic dyes that bind acid nuclei and result in a blue to purple color. (vetstream.com)
Protocols1
- Below, you'll find how to prepare Giemsa solution for various applications, Giemsa staining protocols according to sample type and application, as well as detailed quality information regarding our Giemsa formulation. (merckmillipore.com)
Hematology2
- paramedicsworld.com/hematology- stain ings/ giemsa - stain ing-technique-principle. (reference.com)
- paramedicsworld.com/hematology- stain ings/preparation-of- giemsa - stain -in. (reference.com)
Dilute2
- To achieve desired effects, the stains are used in very dilute solutions ranging from 1:5000 to 1:500000 (Howey, 2000). (wikipedia.org)
- How about staining the formaldehyde-fixed preparation whole with a dilute solution of alcian blue at pH 1? (histosearch.com)
Technique4
- Staining is a technique used to enhance contrast in samples, generally at the microscopic level. (wikipedia.org)
- In order to elucidate the structural chromosome organization of the heterochromatic regions in sheep, we have used C-banding, silver-staining, sequential CDD technique and restriction endonuclease banding. (biomedsearch.com)
- Nucleoli and Nucleolus regions (NORs) in diploid, triploid and tetraploid tea (Camellia sinensis L.) were stained using a simplified technique of silver staining based on the colloidal two-stage method. (biomedsearch.com)
- Pap staining is a very reliable technique. (ihcworld.com)
Tissue2
- Tissue stained with crystal viollet dye and Grams iodine solution, Then decolorized and counterstained. (brainscape.com)
- A selective stain for mast cells in tissue sections is presented. (springer.com)
20001
- 2000) and a Sirius red stain (Aust et al. (histosearch.com)
Specimens1
- The staining results in very transparent cells, so even thicker specimens with overlapping cells can be interpreted. (ihcworld.com)
Mixture1
- Combining H&E with azure II (a mixture of blue cationic thiazine dyes) does not make much sense because the azure and the haemalum both colour nuclei blue, albeit for different reasons. (histosearch.com)
Gustav Giemsa1
- Giemsa stain (/ˈɡiːmzə/), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites. (wikipedia.org)
Hematoxylin1
- Hematoxylin is a dark blue voilet stain. (brainscape.com)
Differentially1
- It differentially stains human and bacterial cells purple and pink respectively. (wikipedia.org)
Shades of b1
- The methylene blue ions are positively charged and stain the acid cell components in varying shades of blue. (horiba.com)
Wright Stain1
- citation needed] Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain. (wikipedia.org)
Bacterial1
- A lignin-degrading bacterial strain capable of decolourising Azure-B dye was identified as lignin peroxidase (LiP) producing strain LD-5. (nih.gov)
Acidic1
- methylene blue: stains blue the acidic components of the cell. (horiba.com)
Supravital1
- Those that enter and stain living cells are called supravital stains (e.g. (wikipedia.org)
Differential stain1
- Giemsa stain is a differential stain that is used to stain the various components of the cells (blood or Aspirated Fluid) and it can be used to study the adherence of pathogenic bacteria to the human cells. (reference.com)
Specimen1
- The liquid is added to the slide before the addition of the organism and a coverslip is placed over the specimen in the water and stain to help contain it within the field of view. (wikipedia.org)
Abnormalities1
- Stain cells to identify abnormalities/pathological changes. (vetstream.com)
Giemsa's2
- Use undiluted Giemsa's solution, stain slides with concentrated Giemsa for 1 min, followed by 2 x 1 min washes with pH 6.8 buffer solution. (merckmillipore.com)
- Use undiluted Giemsa's solution, stain slides with concentrated Giemsa solution for 15 min, followed by 10 sec washes with each of the following solutions: acetic acid (0.1%), distilled water, 2-Propanol (3 times). (merckmillipore.com)
Nuclear3
- Sebaceous carcinomas have significantly increased nuclear staining with p53 and Ki-67 (MIB-1) and reduced levels of BCL2 and p21, in comparison to sebaceous adenomas and sebaceous epitheliomas. (thefreelibrary.com)
- Mutations lead to an abnormally stable but inactive p53 protein that can be detected immunohistochemically by nuclear staining. (thefreelibrary.com)
- A nuclear stain, haematoxylin, is used to stain cell nuclei. (ihcworld.com)
Neurons4
- All rapidly adapting neurons stain more heavily than slowly adapting neurons. (jneurosci.org)
- The exact distribution of peripheral synapses was reconstructed from a 10-μm-long electron micrograph series of the dendritic, somatic, and initial axon regions of acetylcholinesterase-stained VS-3 neurons. (jneurosci.org)
- Here, we describe a protocol to quickly recover, freeze, and section mouse spinal cord to avoid RNA damage by endogenous and exogenous RNases, followed by staining with Azure B in 70 % ethanol to identify the motor neurons while keeping endogenous RNase inhibited. (leica-microsystems.com)
- is then used to capture the stained neurons directly into guanidine thiocyanate lysis buffer, maintaining RNA integrity. (leica-microsystems.com)
Chromatin1
- The chromatin patterns are well visible, the cells from borderline lesions are easier to interpret, the photomicrographs are better, and the stained cells are pretty. (ihcworld.com)
Counterstain1
- A counterstain is stain that makes cells or structures more visible, when not completely visible with the principal stain. (wikipedia.org)
Positively1
- Light microscopy of the corneas revealed deposits of glycosaminoglycans in keratocytes, endothelial cells and extracellularly within the stroma, all staining positively with Alcian blue and periodic acid-Schiff. (harvard.edu)
Blood4
- The first step in the staining operation is to fix the blood by using the alcohol contained in the pure May-Grünwald. (horiba.com)
- When applied to blood cells, the dyes produce multiple colors based on the ionic charge of the stain and the various components of the cell. (horiba.com)
- Principle Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. (thomassci.com)
- It is used to diagnose diseases, such as malaria, which are caused by parasitic infections The collected blood sample is smeared onto a glass slide and stained with Giemsa stain. (reference.com)
Gram2
- Crystal violet stains both Gram positive and Gram negative organisms. (wikipedia.org)
- Variations in Gram Staining Results Various factors influence the results of Gram staining. (reference.com)
Slides3
- Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years. (curehunter.com)
- The results demonstrated that archived Giemsa-stained slides can provide a Leishmania DNA source for performing clinical and epidemiological studies of leishmaniasis . (curehunter.com)
- 264/384) evaluated air-dried slides with a modified Giemsa stain and rendered one TI/NCB combined report (87%, 334/385). (northwestern.edu)
Preparation1
- The novel MycoPerm stain contains plasticizers which help in making a durable long-life preparation. (proscitech.com.au)
Reticulocyte1
- New Methylene Blue and brilliant cresyl blue for reticulocyte staining). (wikipedia.org)
Cutaneous1
- Variations in the staining of cutaneous mast cells. (springer.com)
Tissues2
- In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. (wikipedia.org)
- however, staining can also reveal where certain chemicals or specific chemical reactions are taking place within cells or tissues. (wikipedia.org)
Neutral2
- The neutral components of the cell are stained by both components of the dye producing variable colors. (horiba.com)
- The M. tuberculosis Δ sigF mutant produces reduced levels of cell envelope-associated sulfolipids as assessed by neutral red staining of whole bacteria. (asm.org)
Aqueous2
- It is an aqueous rather than alcohol-based stain (it does not fix cells and therefore it is not a permanent stain). (vin.com)
- Aqueous stain for rapid differential phosphate buffered pH 6.8 used with Rapid stain 11. (proscitech.com.au)
Basophilic2
- Plastids in these sections appeared intensely basophilic when stained with azure B. Both the basophilia and radioactivity were removable with ribonuclease, clearly demonstrating the occurrence of RNA in these organelles. (rupress.org)
- Mature plastids, when stained with azure B, did not appear basophilic under the light microscope. (rupress.org)
Magnification1
- When viewing stained organisms at one hundred times magnification, oil is needed to help create a clearer view due to the distortion of resolution from light refraction which becomes incredibly pronounced at high magnification. (wikipedia.org)
Preparations1
- New methylene blue also is a handy stain for urine sediment and cytologic preparations. (vin.com)
Epithelial2
- Staining of micronuclei in squamous epithelial cells of human oral mucosa. (biomedsearch.com)
- To evaluate the influence of methodologic variables, staining method and sampling, on the frequency of micronuclei scored in squamous epithelial cells of oral mucosa. (biomedsearch.com)
Procedure1
- This video describes the procedure of Alizarin Red S Staining for osteogenesis. (abnova.com)
Propidium iodide1
- Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells). (wikipedia.org)
Squamous1
- Its original role was to stain the small cells of keratinizing squamous cell carcinoma present in sputum. (ihcworld.com)