PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.
Compounds containing dibenzo-1,4-thiazine. Some of them are neuroactive.
A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Structure and inheritance of some heterozygous Robertsonian translocation in man. (1/163)

Banding studies in 25 Robertsonian translocations showed that all could be interpreted as stable dicentrics. The mechanism for their stability is likely to be the proximity of their centromeres but centromeric suppression could also have a role. In many of these dicentric translocations, discontinuous centromeric suppression, as indicated by chromatid separation at one of the centromeric regions, was observed in C-banded preparations. A further observation of undefined relation to the first was that the ratio of the two constitutive centromeric heterochromatin (CCH) regions from the component chromosomes of the translocations was variable in the same translocation type, e.g. t(13;14). It is proposed that this ratio may influence the segregation ratio. Abnormal spermatogenesis is suggested as the likely mechanism for the difference in the proportion of aneuploid offspring in the progeny of maternal and paternal heterozygotes. Neither of the t dic(21;21)s could be interpreted as isochromosomes. It is proposed that Robertsonian fusion translocations be defined as stable, dicentric, whole-arm translocations, with both centromeres in a median position and resulting in the loss of a small acentric fragment during this formation. It is suggested that they occur at high frequency between telocentric or, as in man, certain acrocentric chromosomes because of some intrinsic property of those chromosomes not possessed by metacentric chromosomes and mediated by interphase association of centromeres.  (+info)

Comparison of five methods of malaria detection in the outpatient setting. (2/163)

In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative.  (+info)

Detection of "Candidatus Helicobacter suis" in gastric samples of pigs by PCR: comparison with other invasive diagnostic techniques. (3/163)

Recently, a new 16S ribosomal DNA-based PCR assay was developed for the specific detection of "Candidatus Helicobacter suis" (former "Gastrospirillum suis") in porcine gastric samples. In the present study, this PCR assay was compared to three other invasive diagnostic methods (rapid urease test, immunohistochemistry, histologic analysis by Giemsa staining). Antral stomach samples from 200 slaughterhouse pigs from Belgium and The Netherlands were examined. Bacterial presence was determined in 77% (154 of 200) of the samples by PCR in combination with Southern blot hybridization, 56% (111 of 200) of the samples by immunohistochemistry, 61% (122 of 200) of the samples by urease testing (20 h postinoculation [p.i.]), 36% (71 of 200) of the samples by urease testing (3 h p.i.), and 33% (65 of 200) of the samples by Giemsa staining. The intrinsic specificity of the PCR assay was assessed by Southern blot analysis with an "Candidatus H. suis"-specific probe and sequencing of PCR products. Interassay sensitivity and specificity values were assessed for each test by pairwise comparisons between tests. Agreement between tests was evaluated by calculating Cohen's kappa coefficient. From that analysis, the PCR assay was considered the most reliable benchmark. Microscopic detection of immunohistochemically labeled or Giemsa-stained "Candidatus H. suis" cells in stomach sections proved to be highly specific (100%) but relatively insensitive (72 and 42%, respectively) compared to the PCR assay. A longer incubation time of the urease test improved its sensitivity considerably (74 versus 55%) but was accompanied by a loss of specificity (72 versus 93%). In conclusion, we found the "Candidatus H. suis"-specific PCR assay to be a sensitive and reliable diagnostic method for the detection of "Candidatus H. suis" in the stomachs of pigs and could prove to be a valuable tool for further epidemiological studies both for "Candidatus H. suis"- and for "Helicobacter heilmannii" type 1-related research.  (+info)

Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability. (4/163)

This study was designed to assess whether nucleic acid stains such as propidium iodide and Hoechst 33258 and the cytosolic stain eosin identified equivalent proportions of non-viable cells. Sub-samples of boar spermatozoa stored for up to 72 h, and frozen bull spermatozoa stored in straws and thawed before staining, were exposed to either propidium iodide or Hoechst 33258 alone or in combination. Additional sub-samples were stained with eosin-nigrosin and subsequently with Giemsa. The proportion of non-viable cells identified by propidium iodide alone was equivalent to that observed when it was used in combination with the other fluorescent probe. Similar results were observed for Hoechst 33258. However, direct microscopic examination of sub-samples exposed to both stains revealed that a proportion of spermatozoa stained with propidium iodide did not incorporate Hoechst 33258. This was found consistently in boar and bull spermatozoa under the different experimental conditions used. Quantification showed that the proportion of propidium iodide-positive cells was significantly higher than Hoechst 33258-positive cells. Furthermore, the proportion of propidium iodide-positive cells was higher than cells stained with eosin, but no differences were found between the number of cells stained with Hoechst 33258 or eosin. The proportion of cells stained with propidium iodide was positively correlated with the proportion stained with either Hoechst 33258 or eosin, despite the observation that more cells incorporated propidium iodide. Taken together, these results indicate that there are differences in the ability of fluorescent probes to identify non-viable sperm cells and that this should be considered when staining protocols are used to analyse sperm viability, or when viability is used as a discriminating factor in functional studies, such as those related to acrosomal exocytosis.  (+info)

Histological identification of Helicobacter pylori: comparison of staining methods. (5/163)

AIM: To determine whether two recently described staining methods (the modified McMullen's and the Helicobacter pylori silver stain HpSS methods) used for the histological identification of H pylori organisms are superior to two established techniques (the modified Giemsa and anti-H pylori antibody immunostain) in terms of availability, reproducibility, rapidity, sensitivity, and cost. METHODS: Histological sections from 63 paired gastric biopsies from adult patients previously investigated for dyspepsia were stained with the four methods and these were assessed blindly and independently by two observers. Of the 63 patients, 30 were originally negative in all tests for H pylori infection, 30 were positive, and the remaining three cases had discordant results using a combination of five tests (rapid biopsy urease test, urea breath test, culture, serology, and histology). RESULTS: Interobserver agreement was best with the antibody method (98%), followed by the McMullen's (90%), Giemsa (87%), and HpSS (85%). Of the 60 "gold standard" positive and negative cases, 30 were positive by the modified Giemsa stain, 29 by the McMullen's method, 29 by HpSS, and 30 by the antibody stain. However, there were two false positives with the HpSS method. The modified Giemsa is the cheapest and easiest to perform technically. CONCLUSIONS: When H pylori are present, careful examination will almost always reveal them, whichever of these stains is used. However, the modified Giemsa stain is the method of choice because it is sensitive, cheap, easy to perform, and reproducible.  (+info)

Morphologic aspects of Tetratrichomonas didelphidis isolated from opossums Didelphis marsupialis and Lutreolina crassicaudata. (6/163)

Tetratrichomonas didelphidis (Hegner & Ratcliffe, 1927) Andersen & Reilly, 1965 is a flagellate protozoan found in the intestine, cecum, and colon of Didelphis marsupialis. The parasitic protozoa used in this study was found and isolated in the intestine of opossums in Pavlova starch-containing medium in Florianopolis, State of Santa Catarina, Brazil, from D. marsupialis and Lutreolina crassicaudata. The strains were cultivated in Diamond medium without maltose and with starch solution, pH 7.5 at 28 degrees C. The specimens were stained by the Giemsa method and Heidenhain's iron hematoxylin. The light microscopy study of the trophozoites revealed the same morphologic characteristics as specimens previously described.  (+info)

Atypical Herpes simplex keratitis (HSK) presenting as a perforated corneal ulcer with a large infiltrate in a contact lens wearer: multinucleated giant cells in the Giemsa smear offered a clue to the diagnosis. (7/163)

PURPOSE: To report a case of atypical herpes simplex keratitis initially diagnosed as bacterial keratitis, in a contact lens wearer. RESULTS: Case report of an 18-year-old woman using contact lenses who presented with pain, redness and gradual decrease in vision in the right eye. Examination revealed a paracentral large stromal infiltrate with a central 2-mm perforation. Corneal and conjunctival scrapings were collected for microbiological investigations. Corneal tissue was obtained following penetrating keratoplasty. Corneal scraping revealed no microorganisms. Giemsa stained smear showed multinucleated giant cells. Conjunctival, corneal scrapings and tissue were positive for herpes simplex virus - 1 (HSV) antigen. Corneal tissue was positive for HSV DNA by PCR. CONCLUSIONS: Atypical HSV keratitis can occur in contact lens wearers. A simple investigation like Giemsa stain may offer a clue to the diagnosis.  (+info)

Newly recognized cellular abnormalities in the gray platelet syndrome. (8/163)

The gray platelet syndrome (GPS) is a rare congenital bleeding disorder in which thrombocytopenia is associated with increased platelet size and decreased alpha-granule content. This report describes 3 new pediatric cases presenting with the classical platelet abnormalities of GPS within one family with normal parents. Examination of blood smears of the 3 patients demonstrated not only gray platelets, but also gray polymorphonuclear neutrophils (PMNs) with decreased or abnormally distributed components of secretory compartments (alkaline phosphatase, CD35, CD11b/CD18). Secondary granules were also decreased in number as assayed by immunoelectron microscopy. These data confirm that the secretory compartments in neutrophils were also deficient in this family. Megakaryocytes (MKs) were cultured from the peripheral blood CD34+ cells of the 3 patients for 14 days, in the presence of thrombopoietin and processed for immunoelectron microscopy. Although von Willebrand factor (vWF) was virtually undetectable in platelets, vWF immunolabeling was conspicuous in cultured maturing MKs, particularly within Golgi saccules, but instead of being packaged in alpha-granules, it was released into the demarcation membrane system. In contrast, P-selectin followed a more classical pathway. Double-labeling experiments confirmed that vWF was following an intracellular pathway distinct from the one of P-selectin. In these 3 new cases of GPS, the MKs appeared to abnormally process vWF, with secretion into the extracellular space instead of normal alpha-granule packaging. Furthermore, the secretory compartment of another blood cell line, the neutrophil, was also affected in this family of GPS.  (+info)

'Azure stains' is a term used in pathology to describe a histological staining technique that uses a type of dye called methyl blue, which turns the stained structures a blue-purple color. This technique is often used to stain acid mucins, which are found in various types of tissues and can be indicative of certain medical conditions.

In particular, azure stains are sometimes used to help diagnose certain types of cancer, such as mucoepidermoid carcinoma, a type of salivary gland tumor that produces acid mucins. The staining technique can help pathologists identify the presence and distribution of these mucins within the tumor cells, which can aid in making an accurate diagnosis and determining the best course of treatment.

It's worth noting that there are several different types of histological stains that use various dyes to highlight different structures or features within tissues. Azure stains are just one example of these techniques, and they are typically used in conjunction with other staining methods to provide a comprehensive picture of the tissue being examined.

Phenothiazines are a class of heterocyclic organic compounds that contain a phenothiazine nucleus, which consists of a pair of benzene rings fused to a thiazine ring. They have been widely used in medicine as antipsychotic drugs for the treatment of various mental disorders such as schizophrenia and bipolar disorder.

Phenothiazines work by blocking dopamine receptors in the brain, which helps to reduce the symptoms of psychosis such as hallucinations, delusions, and disordered thinking. They also have sedative and antiemetic (anti-nausea) effects. However, they can cause a range of side effects including extrapyramidal symptoms (involuntary muscle movements), tardive dyskinesia (irreversible movement disorder), and neuroleptic malignant syndrome (a rare but potentially fatal reaction to antipsychotic drugs).

Examples of phenothiazine drugs include chlorpromazine, thioridazine, and promethazine. While they have been largely replaced by newer atypical antipsychotics, phenothiazines are still used in some cases due to their lower cost and effectiveness in treating certain symptoms.

Methylene Blue is a heterocyclic aromatic organic compound with the molecular formula C16H18ClN3S. It is primarily used as a medication, but can also be used as a dye or as a chemical reagent. As a medication, it is used in the treatment of methemoglobinemia (a condition where an abnormal amount of methemoglobin is present in the blood), as well as in some forms of poisoning and infections. It works by acting as a reducing agent, converting methemoglobin back to hemoglobin, which is the form of the protein that is responsible for carrying oxygen in the blood. Methylene Blue has also been used off-label for other conditions, such as vasculitis and Alzheimer's disease, although its effectiveness for these uses is not well established.

It is important to note that Methylene Blue should be used with caution, as it can cause serious side effects in some people, particularly those with kidney or liver problems, or those who are taking certain medications. It is also important to follow the instructions of a healthcare provider when using this medication, as improper use can lead to toxicity.

'Staining and labeling' are techniques commonly used in pathology, histology, cytology, and molecular biology to highlight or identify specific components or structures within tissues, cells, or molecules. These methods enable researchers and medical professionals to visualize and analyze the distribution, localization, and interaction of biological entities, contributing to a better understanding of diseases, cellular processes, and potential therapeutic targets.

Medical definitions for 'staining' and 'labeling' are as follows:

1. Staining: A process that involves applying dyes or stains to tissues, cells, or molecules to enhance their contrast and reveal specific structures or components. Stains can be categorized into basic stains (which highlight acidic structures) and acidic stains (which highlight basic structures). Common staining techniques include Hematoxylin and Eosin (H&E), which differentiates cell nuclei from the surrounding cytoplasm and extracellular matrix; special stains, such as PAS (Periodic Acid-Schiff) for carbohydrates or Masson's trichrome for collagen fibers; and immunostains, which use antibodies to target specific proteins.
2. Labeling: A process that involves attaching a detectable marker or tag to a molecule of interest, allowing its identification, quantification, or tracking within a biological system. Labels can be direct, where the marker is directly conjugated to the targeting molecule, or indirect, where an intermediate linker molecule is used to attach the label to the target. Common labeling techniques include fluorescent labels (such as FITC, TRITC, or Alexa Fluor), enzymatic labels (such as horseradish peroxidase or alkaline phosphatase), and radioactive labels (such as ³²P or ¹⁴C). Labeling is often used in conjunction with staining techniques to enhance the specificity and sensitivity of detection.

Together, staining and labeling provide valuable tools for medical research, diagnostics, and therapeutic development, offering insights into cellular and molecular processes that underlie health and disease.

... in leaf strips of infected plants when stained with either the Orange-Green protein stain or the Azure A nucleic acid stain. ... The Baltimore Method Inclusions of PapMV stained in Azure A. How to Stain for Plant Virus Inclusions (Articles with short ... "Inclusions of PapMV stained in Azure A". Zettler, F.W., Edwardson, J.R. and Purcifull, D.E. 1968. Phytopathology 58: 332 ... "How to Stain for Plant Virus Inclusions". Archived from the original on 2014-10-13. Conover, R.A. 1962. Phytopathology 52: 6. ...
Giemsa's solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available ... Biological stains and staining protocols Histology Leishman stain Microscopy Romanowsky stain Wright's stain Giemsa G (1904 ... Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain. It can be ... Erythrocytes stain pink, platelets show a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale ...
Liu B, on the other hand, is the cationic dye, contains azur I and methylene azure, to stain nucleus and basophilic granules ... Liu's stain (劉氏染色法) is a staining technique used to stain animal cells. It is an improved staining based on Romanowsky stain, ... The staining is done after the water on the smear dried up. Romanowsky stain (Anatomical pathology, Staining, Staining dyes). ... Liu's stain is composed of two dyes, Liu A and Liu B. Liu A is the anionic dye, contains eosin Y to stain cytoplasm as well as ...
These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Azure A or Orange- ... Green stains. The banded inclusions produced by WClMV are disrupted by the process of staining for inclusions. Those of ClYMV ...
These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Azure A or Orange- ... Figure 1. Banned inclusions body (I) of Clover yellow mosaic virus in Vicia faba cells, stained with Azure A.(N = nucleus) The ... Green stains. For many potexviruses these inclusions can be disrupted during the staining procedures. The banded inclusions of ...
... tissues that stain with an azure stain may be referred to as azurophilic. This may also be used for more generalized staining ... Common variants include Wright's stain, Jenner's stain, May-Grunwald stain, Leishman stain and Giemsa stain. All are used to ... A Ziehl-Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the ... Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the ...
... azure stains MeSH D03.494.741.198 - chlorpromazine MeSH D03.494.741.326 - fluphenazine MeSH D03.494.741.470 - mesoridazine MeSH ...
... azure stains MeSH D02.886.369.198 - chlorpromazine MeSH D02.886.369.326 - fluphenazine MeSH D02.886.369.470 - mesoridazine MeSH ...
... protein stain) but not Azure A (nucleic acid stain) . There are four different kinds of Potyvirus inclusions. (CoMV belongs to ... These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Orange-Green ( ...
... protein stain) but not Azure A (nucleic acid stain). Modern detection methods rely primarily on reverse transcription PCR. ... These inclusions can be seen by light microscopy in leaf strips of infected plant tissue stained with Orange-Green ( ...
After washing the probe the remaining DNA is renatured again and stained with Giemsa solution consisting of methylene azure, ... Silver staining: Silver nitrate stains the nucleolar organization region-associated protein. This yields a dark region where ... whereas the schematic karyogram shows the purple hue as typically seen on Giemsa stain (and is a result of its azure B ... and staining with Giemsa stain. Compared to darker regions, the lighter regions are generally more transcriptionally active, ...
... a cab-equipped lead unit Eosin Azure, a polychrome cytoplasmic stain used in the Papanicolaou stain Evolutionary algorithm, an ...
B. There are two types of inclusions found in leaf strips stained with Azure A (nucleic acid stain), one is vacuolate (Vac Inc ...
One visitor to the synagogue observed, upon entering through a quiet courtyard, viewing the azure stained glass windows and ...
Azure and Gold: The Stained-Glass Windows of Thomas Denny (2017) ISBN 1848222289 v t e (Webarchive template wayback links, ... to cut out and lead the stained glass, so Denny can focus on painting and firing the stained glass. "Stained Glass Windows - ... He achieves this effect by acid etching and silver staining each small piece of glass. He has been responsible for some 60 ... Thomas Denny (born 1956) is a contemporary British painter and stained glass artist. Denny was born in London, son of Sir ...
Giemsa stain is composed of "Azure II" and eosin Y with methanol and glycerol as the solvent. "Azure II" is thought to be a ... Wright's stain can be used alone or in combination with the Giemsa stain, which is known as the Wright-Giemsa stain. Wright's ... Romanowsky staining, also known as Romanowsky-Giemsa staining, is a prototypical staining technique that was the forerunner of ... The May-Grünwald-Giemsa stain is a two step procedure that includes first staining with May-Grünwald stain, which does not ...
Methylene blue Azure A pH buffer Wright Giemsa stain Papanicolaou stain Silverman JF, Frable WJ (1990). "The use of the diff- ... Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining ... There are generic brands of such stain, and the trade name is sometimes used loosely to refer to any such stain (much as "Coke ... Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It ...
It can also be used to stain lysosome in blood smears, and is often used in Giemsa stain. v t e (Articles without InChI source ... Azure A is an organic compound with the chemical formula C14H14ClN3S. It is a light blue to dark blue dye. It is used as a ...
Field's stain consists of two parts - Field's stain A is methylene blue and Azure 1 dissolved in phosphate buffer solution; ... Field stain is a histological method for staining of blood smears. It is used for staining thick blood films in order to ... Field stain recipe (thelabrat.com) v t e (Histopathology, Staining, Romanowsky stains, All stub articles, Pathology stubs). ... Field stain. Granuloma. Field stain. Chunge, CN.; Ngige, S.; Bwibo, CR.; Mulega, PC.; Kilonzo, JF.; Kibati, F.; Owate, J. (Aug ...
If only synthetic Azure B and Eosin Y is used, it may serve as a standardized Giemsa stain; but, without methylene blue, the ... which are Azure B, Azure A, Azure C, and thionine, respectively. This is the basis of the basophilic part of the spectrum of ... such as Wright's stain and Jenner's stain. Since it is a temporary staining technique, methylene blue can also be used to ... The presence of free dye solution can be checked with stain test on a filter paper. In biology, methylene blue is used as a dye ...
... azure, vert, and purpure. The stains are considered colours for the purposes of the rule. The rule of tincture does not apply ... French civic heraldry, with its frequent chiefs of France (i.e. "Azure, three fleurs-de-lys or", anciently "Azure, semée-de-lys ... They are then represented azure, the term being 'armed and langued' of such and such a colour." Furthermore, Fox-Davies wrote ... with a trimount azure on a field gules The arms of Trøndelag place a cross Or on an argent field, violating the rule of ...
ICSH reference method for staining of blood and bone marrow films by AZure B and Eosin Y (Romanowsky stain) INTERNATIONAL ... Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears. It is generally used to ... It is a version of the Romanowsky stain, and is thus similar to and partially replaceable by Giemsa stain, Jenner's stain, and ... It has been documented that Leishman staining is more sensitive than Field's stain and as good as fluorescent stains for ...
Shown either side of Margaret is a shield bearing the arms of Yeo: Argent, a chevron sable between three drakes azure, which ... Per research conducted by Sheila Yeo of the Yeo Society, based on stained glass depictions of Yeo arms in churches of ... a chevron between three mallards azure (Pole). Vivian indicates that this is Rolle's son Samuel's father-in-law. Plans of Old ... arms can be seen in stained glass reset in incomplete form in the vestry window. Robert Rolle (died 1633) (eldest son), who in ...
It has some late medieval stained glass and a Jacobean pulpit. Two stained glass windows were made by Kempe Studios in 1891 and ... Shown either side of Margaret is a shield bearing the arms of Yeo: Argent, a chevron sable between three drakes azure, which ... on a fesse dancette between three billets azure each charged with a lion rampant of the first three bezants a mullet for ... on a fesse indented azure between three billets of the second each charged with a lion rampant of the first three bezants. ...
Stained with Crimson has a ruby scarab ring; Malice in Saffron has a topaz crucifix; and Empires of Azure has a sapphire ... Empires of Azure is narrated by St Jean, a journalist. The story is about Louis de Jenier, a celebrated female impersonator in ... Each jewel is fateful for its possessor". Stained With Crimson is the story of Andre St. Jean, a poet in 19th-century Paradys, ... The Book of the Damned consists of three short novellas set in Paradys, an alternate world version of Paris: Stained with ...
... a lion passant argent a label of three points azure) where they are also shown in a stained glass window dedicated to Anne ...
... methylene azure) that produced a surprising hue unattributable to either staining component: a shade of purple. Malachowski ... Malachowski's staining technique was one of the most significant technical advances in the history of malaria. In 1891, Paul ... The term μίασμα (Greek for miasma: "stain" or "pollution") was coined by Hippocrates of Kos who used it to describe dangerous ... This discovery remained controversial until the development of the oil immersion lens in 1884 and of superior staining methods ...
The vast single space houses abstract stained glass in the horizontal and vertical window strips, the colours of which are ... dominated by azure blue. Behind the altar is a semicircular coved concrete wall, surrounded by vertical and horizontal window ...
... methylene blue and azure A etc.), which are metachromatic due to switching from monomeric to stacked aggregates, Alcian blue is ... Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods. Alcian blue can be used ... However prolonged staining (few days at 25 °C) or DNA denaturing conditions may allow Alcian blue to also stain the nucleus. ... At pH 1.0 it stains only sulfated polysaccharides and at pH 2.5 also stains carboxyl group containing sugars such as sialic ...
... azure B, and methylene blue (some commercial preparations combine solutions to simplify staining). The May-Grünwald stain, ... The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains ... White blood cells stained with Wright's stain: Diff-Quik Leishman stain List of histologic stains that aid in diagnosis of ... Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of ...
St. Hedwig Catholic Church - Toledo, OH (circa 1920). John W. Winterich & Associates, Inc.-Contractor. Replacement of all protection glass with frame restoration and leaded window repairs including:. ...
... in leaf strips of infected plants when stained with either the Orange-Green protein stain or the Azure A nucleic acid stain. ... The Baltimore Method Inclusions of PapMV stained in Azure A. How to Stain for Plant Virus Inclusions (Articles with short ... "Inclusions of PapMV stained in Azure A". Zettler, F.W., Edwardson, J.R. and Purcifull, D.E. 1968. Phytopathology 58: 332 ... "How to Stain for Plant Virus Inclusions". Archived from the original on 2014-10-13. Conover, R.A. 1962. Phytopathology 52: 6. ...
Careful microscopic examination of blood smears or buffy-coat preparations stained with eosin-azure-type dyes (e.g., Wright- ... Acridine orange stain of Rickettsia africae isolated in Vero E6 cells from an eschar biopsy specimen from a patient with ... Immunoperoxidase and immunofluorescent staining of Rickettsia rickettsii in skin biopsies. A comparative study. Arch Pathol Lab ... No visible dental staining in children treated with doxycycline for suspected Rocky Mountain spotted fever. J Pediatr 2015;166: ...
One-micrometer-thick sections were mounted on glass slides and either stained with methylene blue-azure II (Richardson et al., ...
... identified via GFP expression are labeled with the Azure LUT), and nuclei stained by DAPI is labeled with the Electric Indigo ... identified via GFP expression are labeled with the Azure LUT), and nuclei stained by DAPI is labeled with the Electric Indigo ... Immune cells(azure LUT, anti-CD45 antibody) and type II ATs (amber LUT, anti-proSP-C antibody) are identified via ... Colors: pro-SPC is labeled with the Amber LUT, Mtb is labeled with the Spring Green LUT, and CD45 is labeled with the Azure LUT ...
6] An iron stain, such as Perls Prussian blue stain, should be used to detect azure granules around the blood vessels and ...
Azure eyes stained red by the flow of blood from his hair. Mixed with others and his own having killed so many people. His eyes ...
A deep iridescent pressed multichrome eyeshadow from our Stained Glass Collection. It has a medium, cool tan base that shifts ... Home › Stained Glass Collection › Azure , Deep Iridescent Multichrome Eyeshadow .selector-wrapper select, .product-variants ... Azure: A medium, cool tan base that shifts teal-blue-purple.. Includes: One multichrome shadow housed in a single shadow ... STAINED GLASS COLLECTION CATEGORY. The Deep Iridescent Multichromes: A multipurpose shadow featuring a tanned base. They are ...
... also known as Romanowsky-Giemsa staining, is a classic staining technique that paved the way for several different but similar ... Giemsa stain: The Giemsa stain consists of "Azure II" and eosin Y, with methanol and glycerol serving as solvents. "Azure II" ... The most common Romanowsky stains include Wright-Giemsa stain, May-Grünwald stain, Leishman stain, Field stain, Supravital ... May-Grünwald stain: This is a modified Wright-Giemsa stain and is used for the same purposes as Wright-Giemsa stain. Staining ...
azurophil, azurophile - Staining readily with an azure dye, denoting especially the hyperchromatin and reddish purple granules ... Azurophil - is the term used to refer to objects that are readily staining with an azure dye. [EMedicineDictionary,azurophil] ... az·u·ro·phil·ia (azh″u ro filґe ə) 1. the quality of staining well with blue aniline dyes. 2. the presence of azurophil ...
Enhance the look of your home or business with acid stained concrete. ... multi-colored surface with our selection of concrete acid stains. Available in a variety of colors to suit any style. ... Avocado, Azure Blue, and Seagrass stains are sensitive to moisture and can produce a black, spotty effect. These colors are not ... Can I use EverStain™ Acid Stain for this project?. An acid stain trial kit is a great way to see if EverStain™ is right for ...
... and stain red by azure-eosin stains. ( NCI )] (UMLS (NCI) C0333828) =Cell Component 92. authorized by [A relationship in which ...
STAIN. Wrights Stain. (mixture of methylene blue,. azure II, and eosin). FIXATIVE ...
Glory, azure & gold : the stained-glass windows of Thomas Denny Published: (2016) ...
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Aerial images showed the scale of the damage, with huge stretches of azure seas stained inky black by the spill. ...
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G stands for Geimsa stain. The stain is mixture of cationic thiazine dyes, azure B, and anionic eosin dyes. ... R bands are obtained by preheating the chromosome at at 88°C in a buffer solution followed by staining with Giemsa stain (GC ... This is the second most commonly used method for staining chromosome. *The Geimsa stain also binds to AT rich region and ... The chromosome shows different pattern of bands based on the type of pretreatment and stain are used. Hence, the staining ...
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  • Romanowsky staining, also known as Romanowsky-Giemsa staining, is a classic staining technique that paved the way for several different but similar stains widely used in haematology (the study of blood) and cytopathology (the study of cells) (the study of diseased cells). (microbiologynote.com)
  • In addition to the Romanowsky-type stains, the Giemsa, Jenner, Wright, Field, May-Grünwald, and Leishman stains are linked to or derived from the Romanowsky-type stains. (microbiologynote.com)
  • it can be used on its own or in tandem with the Giemsa stain to create the Wright-Giemsa stain. (microbiologynote.com)
  • The "reddish-purple" colour of the cytoplasmic granules is become more noticeable when Giemsa is added to Wright's stain. (microbiologynote.com)
  • U.S. pathologists frequently use Romanowsky-type stains like Wright's and Wright-Giemsa for staining blood and bone marrow films. (microbiologynote.com)
  • This is a modified Wright-Giemsa stain and is used for the same purposes as Wright-Giemsa stain. (microbiologynote.com)
  • Staining with the May-Grünwald stain, which does not generate the Romanowsky effect, is the first stage of the May-Grünwald-Giemsa stain, which entails a second staining phase with the Giemsa stain, which does cause the Romanowsky effect. (microbiologynote.com)
  • The Giemsa stain consists of "Azure II" and eosin Y, with methanol and glycerol serving as solvents. (microbiologynote.com)
  • Azure II" is believed to be a combination of azure B (which Giemsa referred to as "azure I") and methylene blue, while the precise composition of "azure I" is a trade secret. (microbiologynote.com)
  • Giemsa stain is regarded as the gold standard for detecting and identifying the malaria parasite. (microbiologynote.com)
  • The Giemsa staining technique may need pre-treatment with trypsin. (biokimicroki.com)
  • C banding is obtained by staining chromosome with Giemsa stain. (biokimicroki.com)
  • R bands are obtained by preheating the chromosome at at 88°C in a buffer solution followed by staining with Giemsa stain (GC bonds melts around 105°C. (biokimicroki.com)
  • In the rat skin MN test, pefcalcitol showed positive when specimens were stained with Giemsa, whereas neither an in vitro chromosome aberration test in CHL cells nor an in vivo bone marrow MN test in rats indicated clastogenicity. (biomedcentral.com)
  • The MN-like granules that had been stained by Giemsa were not stained by AO, and AO-stained specimens indicated that pefcalcitol did not increase the frequency of micronucleated (MNed) cells. (biomedcentral.com)
  • The present study demonstrated that Giemsa staining gave a misleading positive result in the skin MN test, because Giemsa stained keratohyalin granules. (biomedcentral.com)
  • A significant increase of MNed cells was observed in the skin MN test with Giemsa staining, but neither clastogenic nor mutagenic activity of the compound was suggested in any other assays. (biomedcentral.com)
  • Because it was unlikely that the test compound was clastogenic to rat skin cells only, we re-stained the Giemsa slides with acridine orange (AO), which can react with DNA to cause specific fluorescence. (biomedcentral.com)
  • Re-staining with AO revealed that the great majority of the micronuclei (MNi) that had taken up Giemsa stain after treatment with pefcalcitol did not exhibit DNA-specific fluorescence, even though most of the MNi derived from vehicle- or mitomycin C (MMC)-treated rats were stained with AO. (biomedcentral.com)
  • Giemsa and Leishman stains provide identical contrasting coloration as Hematoxylin and Eosin (H-E) with the both advantages of that they can be used for staining non-histological sectionsand rapid preparation.The primary aim of the study was to compare the efficiency of digestion and Baermann techniques for detection of ML in muscles. (bvsalud.org)
  • Muscle samples were prepared for staining with Giemsa, Leishman and H-E. Results: Number of larvae recovered by Baermann was higher recovered by digestion method (P Ë 0.5). (bvsalud.org)
  • Leishman stain is superior to Giemsa stain as it takes less time. (bvsalud.org)
  • The dyes, which include Eosin Y and oxidised methylene blue (azure), produce neutral stains. (microbiologynote.com)
  • Wright's stain gets its name from James Homer Wright, who in 1902 reported a method for making polychromed methylene blue by combining it with eosin Y and applying heat. (microbiologynote.com)
  • The eosinate of polychromed methylene blue and eosin Y are dissolved in methanol to create Leishman's stain. (microbiologynote.com)
  • The stain is mixture of cationic thiazine dyes, azure B, and anionic eosin dyes. (biokimicroki.com)
  • The Geimsa stain also binds to AT rich region and appears dark purple bands due to precipitation of eosin molecule with the thiazine molecules. (biokimicroki.com)
  • And by the action of oxidative demethylation, oxidised unmethylated methylene blue can be used to create stains of the Romanowsky type. (microbiologynote.com)
  • Methylene blue disintegrates into several coloured stains as a result, and some of these stains are responsible for the Romanowsky phenomenon. (microbiologynote.com)
  • In 1901, William Leishman created a stain comparable to Louis Jenner's, but with polychromed methylene blue instead of pure methylene blue. (microbiologynote.com)
  • Secondary aim was detection of ML by Geimsa and Leishman stain and their comparison with routinely used stain H-E. Methods: Muscles from 36 mice orally infected with Trichinella spiralis encysted larvae were examined. (bvsalud.org)
  • Leishman stain was the best stain for rapid detection of ML after 30 min followed in validity by Geimsa then H-E. Conclusion: Baermann concentration technique showed higher sensitivity than digestion method. (bvsalud.org)
  • G stands for Geimsa stain. (biokimicroki.com)
  • Unlike Quinacrine stain, Geimsa is non flourescent hence UV light is not required to observe the bands. (biokimicroki.com)
  • The azures are blue-purple because they are basic dyes that bond to the acid nucleus. (microbiologynote.com)
  • the quality of staining well with blue aniline dyes. (en-academic.com)
  • E-F ) Confocal microscope images of an uninfected LoC stained to visualize nuclei (blue), actin (gray), and surfactant (green, anti-pro-SPC antibody) verifies that confluency of epithelial ( E ) and endothelial ( F ) layers is maintained at the air-liquid interface over 7 days. (elifesciences.org)
  • Romanowsky stains are a group of staining techniques used in hematology, pathology and histology to visualize and differentiate blood cells, tissue structures, and microorganisms. (microbiologynote.com)
  • azurophil, azurophile - Staining readily with an azure dye, denoting especially the hyperchromatin and reddish purple granules of certain blood cells. (en-academic.com)
  • For chromosome staining, the cells are arrested at metaphase by using chemical like mytomycin C or colchisine. (biokimicroki.com)
  • It is one of the simplest method for staining chromosome. (biokimicroki.com)
  • On quinacrine staining, the particular chromosome shows characteristic patterns of banding and they are reproducible. (biokimicroki.com)
  • This is the second most commonly used method for staining chromosome. (biokimicroki.com)
  • Hence, in order to stain, the chromosome need to be treated with acid, saline and alkali for denaturation. (biokimicroki.com)
  • The chromosome shows different pattern of bands based on the type of pretreatment and stain are used. (biokimicroki.com)
  • In this pendant Meyda Tiffany uses colorful interlocking blocks of Willow Green, Chili Red and Azure Blue to form stylized wheat stalks on a Beige Cream background. (meyda.com)
  • SamaN Water-based Stain is a wiping stain recommended for interior wood furniture and woodworks. (torontopaintstore.com)
  • Mature virions form banded inclusions that can be seen in leaf strips of infected plants when stained with either the Orange-Green protein stain or the Azure A nucleic acid stain. (wikipedia.org)
  • EverStain™ Acid Stain offers a lasting way to color concrete surfaces, ensuring durability, longevity, and a natural appearance. (directcolors.com)
  • I've just applied acid stain to my concrete and after it dried, it barely has any color. (directcolors.com)
  • Not only have you put in the work to prepare for concrete stain, but you've also applied the acid stain, neutralized, and scrubbed the remaining residue. (directcolors.com)
  • To elucidate the causes of the discrepancy, the MN specimens were re-stained with acridine orange (AO), a fluorescent dye specific to nucleic acid, and the in vivo clastogenicity of the compound in rat skin was re-evaluated. (biomedcentral.com)
  • This comprehensive kit is perfect for DIY enthusiasts, containing protective gear, cleaning supplies, a gallon of stain, 4-ounce ProClean Neutralizer™, application tools, and a gallon of sealer. (directcolors.com)
  • Backer Rod - Flexible foam rod that is compressed around a window to take up space prior to caulking the stained glass or protective glass. (pittsburghstainedglassstudios.com)
  • Hence, the stain binds to GC region (euchromatin - less condensed DNA) and appears bright in color. (biokimicroki.com)
  • Back Painting - Vitreous paints and glazes applied to the backside of the glass to exaggerate depth of color, create a softer less defined image, or to apply a stain that will alter the transmitted color. (pittsburghstainedglassstudios.com)
  • Hand-cut and hand-soldered pieces of authentic stained glass using Meyda Tiffany copperfoil construction technique. (meyda.com)
  • The handcrafted stained glass shade framed in Bronzed Jade glass is suspended from a Mahogany Bronze hand finished chain and canopy. (meyda.com)
  • Bead - A small strip of wood or metal used to mechanically hold a stained glass window or storm window into the supporting frame. (pittsburghstainedglassstudios.com)
  • The technique is named after Russian physician Dmitri Leonidovich Romanowsky (1861-1921), who was among the first to realise its potential as a blood stain. (microbiologynote.com)
  • Aerial images showed the scale of the damage, with huge stretches of azure seas stained inky black by the spill. (dhakatribune.com)
  • The stripped-back base is crafted from solid Linden wood with an LED source concealed within the ball- or teardrop-shaped piece, which are left natural or stained in black lacquer. (azuremagazine.com)
  • Hence, the staining techniques are used for identification and comparison of chromosomes. (biokimicroki.com)
  • Romanowsky stains are used to distinguish cells for microscopic study in pathological materials, particularly blood and bone marrow films, and to detect blood parasites such as malaria. (microbiologynote.com)
  • Romanowsky staining is effective because it generates a range of colours, each of which can be used to identify a specific subset of cells. (microbiologynote.com)
  • It is used to stain peripheral blood smears and bone marrow aspirates, and it can be used to differentiate leukocytes, erythrocytes, platelets and microorganisms. (microbiologynote.com)
  • This stain is used to stain blood smears, and it is especially useful for the visualization of parasites such as Plasmodium and Trypanosoma. (microbiologynote.com)
  • It is important to test the effectiveness of such stain removal products before using them. (windowsazurecat.com)
  • Some people use stain and chemical removers or solutions to clean carpets. (windowsazurecat.com)
  • Acids are found in many chemical stain removers, which can damage rug fibers. (windowsazurecat.com)
  • They also make use of special solutions to remove stains and restore the original look of carpets. (windowsazurecat.com)
  • SamaN stains are perfectly stabilized water-based solutions. (torontopaintstore.com)
  • The Au Naturel Collection pays respect to timeless, naturally-stained honey wood tones with subtle graining and graceful movement in this moderniz. (americasfloorsource.com)