Azospirillum brasilense
Azospirillum
Gram-Negative Aerobic Bacteria
Glutamate Synthase
Nitrogen Fixation
PII Nitrogen Regulatory Proteins
Nitrogenase
Dinitrogenase Reductase
Spirillum
Polyesters
Plant Roots
Gene Expression Regulation, Bacterial
Triticum
Quaternary Ammonium Compounds
Ammonia
Chemotaxis
Glutamate-Ammonia Ligase
Pennisetum
Encyclopedias as Topic
Azospirillum lipoferum
Muramic Acids
Cell Wall
Uridine Diphosphate N-Acetylmuramic Acid
Aminoacyltransferases
Phloroglucinol
Nitric Oxide
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
Botany
Hepatitis, Infectious Canine
Dog Diseases
Cryoelectron Microscopy
Models, Molecular
Amino Acid Transport Systems, Acidic
Rhizobium
Plants, Genetically Modified
Nitrogen
Biosynthesis of indole-3-acetic acid in Azospirillum brasilense. Insights from quantum chemistry. (1/107)
Quantum chemical methods AM1 and PM3 and chromatographic methods were used to qualitatively characterize pathways of bacterial production of indole-3-acetic acid (IAA). The standard free energy changes (delta G(o)'sum) for the synthesis of tryptophan (Trp) from chorismic acid via anthranilic acid and indole were calculated, as were those for several possible pathways for the synthesis of IAA from Trp, namely via indole-3-acetamide (IAM), indole-3-pyruvic acid (IPyA), and indole-3-acetonitrile (IAN). The delta G(o)'sum for Trp synthesis from chorismic acid was -402 (-434) kJ.mol-1 (values in parentheses were calculated by PM3). The delta G(o)'sum for IAA synthesis from Trp were -565 (-548) kJ.mol-1 for the IAN pathway, -481 (-506) kJ.mol-1 for the IAM pathway, and -289 (-306) kJ.mol-1 for the IPyA pathway. By HPLC analysis, the possibility was assessed that indole, anthranilic acid, and Trp might be utilized as precursors for IAA synthesis by Azospirillum brasilense strain Sp 245. The results indicate that there is a high motive force for Trp synthesis from chorismic acid and for IAA synthesis from Trp, and make it unlikely that anthranilic acid and indole act as the precursors to IAA in a Trp-independent pathway. (+info)Azospirillum irakense produces a novel type of pectate lyase. (2/107)
The pelA gene from the N2-fixing plant-associated bacterium Azospirillum irakense, encoding a pectate lyase, was isolated by heterologous expression in Escherichia coli. Nucleotide sequence analysis of the region containing pelA indicated an open reading frame of 1,296 bp, coding for a preprotein of 432 amino acids with a typical amino-terminal signal peptide of 24 amino acids. N-terminal amino acid sequencing confirmed the processing of the protein in E. coli at the signal peptidase cleavage site predicted by nucleotide sequence analysis. Analysis of the amino acid sequence of PelA revealed no homology to other known pectinases, indicating that PelA belongs to a new pectate lyase family. PelA macerates potato tuber tissue, has an alkaline pH optimum, and requires Ca2+ for its activity. Of several divalent cations tested, none could substitute for Ca2+. Methyl-esterified pectin (with a degree of esterification up to 93%) and polygalacturonate can be used as substrates. Characterization of the degradation products formed upon incubation with polygalacturonate indicated that PelA is an endo-pectate lyase generating unsaturated digalacturonide as the major end product. Regulation of pelA expression was studied by means of a translational pelA-gusA fusion. Transcription of this fusion is low under all growth conditions tested and is dependent on the growth phase. In addition, pelA expression was found to be induced by pectin. An A. irakense pelA::Tn5 mutant still displayed pectate lyase activity, suggesting the presence of multiple pectate lyase genes in A. irakense. (+info)Involvement of outer-membrane proteins in the aggregation of Azospirillum brasilense. (3/107)
A bioassay was developed to investigate biological factors involved in the aggregation of Azospirillum brasilense strain Cd. Cells were grown for 24 h under aggregation-inducing and non-aggregation-inducing conditions (high and low C:N, respectively) and sonicated for 20 s. The cells were washed by centrifugation and resuspended in potassium phosphate buffer containing the two types of sonication extract. A greater extent of aggregation and higher flocculation were observed after 2-3 h incubation in the presence of sonicates from cells grown at high C:N (H-cells) compared to cells grown at low C:N. Flocculation did not occur after incubation of these cells in phosphate buffer. Boiled or proteinase K-treated sonicates originating from H-cells had lower aggregation-inducing capacity. After fractionation of the crude sonicate, both the outer-membrane protein (OMP) and the total membrane (mostly OMP) fractions possessed relatively high aggregation specific activities. The aggregation-inducing capacity of the OMP fraction strongly correlated with its protein concentration in the bioassay. Treatment of this fraction with proteinase K also decreased its aggregation-inducing activity. These findings suggest that OMPs are involved in the aggregation process of cells of A. brasilense. (+info)The ntrB and ntrC genes are involved in the regulation of poly-3-hydroxybutyrate biosynthesis by ammonia in Azospirillum brasilense Sp7. (4/107)
Azospirillum brasilense Sp7 and its ntrA (rpoN), ntrBC, and ntrC mutants have been evaluated for their capabilities of poly-3-hydroxybutyrate (PHB) accumulation in media with high and low ammonia concentrations. It was observed that the ntrBC and ntrC mutants can produce PHB in both low- and high-C/N-ratio media, while no significant PHB production was observed for the wild type or the ntrA mutant in low-C/N-ratio media. Further investigation by fermentation analysis indicated that the ntrBC and ntrC mutants were able to grow and accumulate PHB simultaneously in the presence of a high concentration of ammonia in the medium, while little PHB was produced in the wild type and ntrA (rpoN) mutant during active growth phase. These results provide the first genetic evidence that the ntrB and ntrC genes are involved in the regulation of PHB synthesis by ammonia in A. brasilense Sp7. (+info)Increased growth of the microalga Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense. (5/107)
Coimmobilization of the freshwater microalga Chlorella vulgaris and the plant-growth-promoting bacterium Azospirillum brasilense in small alginate beads resulted in a significantly increased growth of the microalga. Dry and fresh weight, total number of cells, size of the microalgal clusters (colonies) within the bead, number of microalgal cells per cluster, and the levels of microalgal pigments significantly increased. Light microscopy revealed that both microorganisms colonized the same cavities inside the beads, though the microalgae tended to concentrate in the more aerated periphery while the bacteria colonized the entire bead. The effect of indole-3-acetic acid addition to microalgal culture prior to immobilization of microorganisms in alginate beads partially imitated the effect of A. brasilense. We propose that coimmobilization of microalgae and plant-growth-promoting bacteria is an effective means of increasing microalgal populations within confined environments. (+info)Functional properties of recombinant Azospirillum brasilense glutamate synthase, a complex iron-sulfur flavoprotein. (6/107)
Azospirillum brasilense glutamate synthase is a complex iron-sulfur flavoprotein that catalyses the NADPH-dependent reductive transfer of glutamine amide group to the C(2) carbon of 2-oxoglutarate to yield L-glutamate. Its catalytically active alphabeta protomer is composed of two dissimilar subunits (alpha subunit, 164.2 kDa; beta subunit, 52.3 kDa) and contains one FAD (at Site 1, the pyridine nucleotide site within the beta subunit), one FMN (at Site 2, the 2-oxoglutarate/L-glutamate site in the alpha subunit) and three different iron-sulfur clusters (one 3Fe-4S center on the alpha subunit and two 4Fe-4S clusters of unknown location). A plasmid harboring the gltD and gltB genes, the genes encoding the glutamate synthase beta and alpha subunits, respectively, each one under the control of the T7/lac promoter of pET11a was found to be suitable for the overproduction of glutamate synthase holoenzyme in Escherichia coli BL21(DE3) cells. Recombinant A. brasilense glutamate synthase could be purified to homogeneity from overproducing E. coli cells by ion exchange chromatography, gel filtration and affinity chromatography on a 2',5' ADP-Sepharose 4B column. The purified enzyme was indistinguishable from that prepared from Azospirillum cells with respect to cofactor content, N-terminal sequence of the subunits, aggregation state, kinetic and spectroscopic properties. The study of the recombinant holoenzyme allowed us to establish that the tendency of glutamate synthase to form a stable (alphabeta)4 tetramer at high protein concentrations is a property unique to the holoenzyme, as the isolated beta subunit does not oligomerize, while the isolated glutamate synthase alpha subunit only forms dimers at high protein concentrations. Furthermore, the steady-state kinetic analysis of the glutamate synthase reaction was extended to the study of the effect of adenosine-containing nucleotides. Compounds such as cAMP, AMP, ADP and ATP have no effect on the enzyme activity, while the 2'-phosphorylated analogs of AMP and NADP(H) analogs act as inhibitors of the reaction, competitive with NADPH. Thus, it can be ruled out that glutamate synthase reaction is subjected to allosteric modulation by adenosine containing (di)nucleotides, which may bind to the putative ADP-binding site at the C-terminus of the alpha subunit. At the same time, the strict requirement of a 2'-phosphate group in the pyridine nucleotide for binding to glutamate synthase (GltS) was established. Finally, by comparing the inhibition constants exhibited by a series of NADP+ analogs, the contribution to the binding energy of the various parts of the pyridine nucleotide has been determined along with the effect of substituents on the 3 position of the pyridine ring. With the exception of thio-NADP+, which binds the tightest to GltS, it appears that the size of the substituent is the factor that affects the most the interaction between the NADP(H) analog and the enzyme. (+info)Mutants with enhanced nitrogenase activity in hydroponic Azospirillum brasilense-wheat associations. (7/107)
The effect of a mutation affecting flocculation, differentiation into cyst-like forms, and root colonization on nitrogenase expression by Azospirillum brasilense is described. The gene flcA of strain Sp7 restored these phenotypes in spontaneous mutants of both strains Sp7 and Sp245. Employing both constitutive pLA-lacZ and nifH-lacZ reporter fusions expressed in situ, the colony morphology, colonization pattern, and potential for nitrogenase activity of spontaneous mutants and flcA Tn5-induced mutants were established. The results of this study show that the ability of Sp7 and Sp245 mutant strains to remain in a vegetative form improved their ability to express nitrogenase activity in association with wheat in a hydroponic system. Restoring the cyst formation and colonization pattern to the spontaneous mutant Sp7-S reduced nitrogenase activity rates in association with plants to that of the wild-type Sp7. Although Tn5-induced flcA mutants showed higher potentials for nitrogenase expression than Sp7, their potentials were lower than that of Sp7-S, indicating that other factors in this strain contribute to its exceptional nitrogenase activity rates on plants. The lack of lateral flagella is not one of these factors, as Sp7-PM23, a spontaneous mutant impaired in swarming and lateral-flagellum production but not in flocculation, showed wild-type nitrogenase activity and expression. The results also suggest factors of importance in evolving an effective symbiosis between Azospirillum and wheat, such as increasing the availability of microaerobic niches along the root, increased supply of carbon sources by the plant, and the retention of the bacterial cells in vegetative form for faster metabolism. (+info)Energy taxis is the dominant behavior in Azospirillum brasilense. (8/107)
Energy taxis encompasses aerotaxis, phototaxis, redox taxis, taxis to alternative electron acceptors, and chemotaxis to oxidizable substrates. The signal for this type of behavior is originated within the electron transport system. Energy taxis was demonstrated, as a part of an overall behavior, in several microbial species, but it did not appear as the dominant determinant in any of them. In this study, we show that most behavioral responses proceed through this mechanism in the alpha-proteobacterium Azospirillum brasilense. First, chemotaxis to most chemoeffectors typical of the azospirilla habitat was found to be metabolism dependent and required a functional electron transport system. Second, other energy-related responses, such as aerotaxis, redox taxis, and taxis to alternative electron acceptors, were found in A. brasilense. Finally, a mutant lacking a cytochrome c oxidase of the cbb(3) type was affected in chemotaxis, redox taxis, and aerotaxis. Altogether, the results indicate that behavioral responses to most stimuli in A. brasilense are triggered by changes in the electron transport system. (+info)
EMDB-10104: Structure of Azospirillum brasilense Glutamate Synthase in a4b4 o... - Yorodumi
Azospirillum brasilense - Wikipedia
Caracterização de uma fosforribohidrolase do tipo lonely guy de Azospirillum brasilense, com uma possível ação na biossíntese...
Azospirillum brasilense: Laboratory Maintenance and Genetic Manipulation - Current Protocols
Characterization of an Azospirillum brasilense Tn5 Mutant with Enhanced Nitrogen Fixtation | Springer for Research & Development
KEGG PATHWAY: Peptidoglycan biosynthesis - Azospirillum brasilense Sp245
Aerobic Nitric Oxide Production by Azospirillum brasilense Sp245 and Its Influence on Root Architecture in Tomato
Plasmid Rearrangements and Changes in Cell-Surface Architecture and Social Behavior of Azospirillum brasilense | SpringerLink
Colonization and nitrogenase activity of Triticum aestivum (cv...
Xtreme Gardening Nitrogen Fixing Bacteria | Wallace Organic Wonder
Oviposition Attractancy of Bacterial Culture Filtrates: response of Culex quinquefasciatus
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Fatty acid analysis of four Azospirillum species reveals three groups | SpringerLink
Isolation and Biochemical characterization of efficient strains of Rhizobium and Azospirillum from wheat and maize under water...
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Development and production of cabbage (Brassica oleracea var. Royal vantage) under different osmotic pressures and...
NITROGEN FIXATION AND NITRATE RESPIRATION IN AZOSPIRILLUM | Biochemical Society Transactions
AZL a01600 - DotU domain-containing protein - Azospirillum sp. (strain B510) - AZL a01600 gene & protein
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Effect of Klebsiella pneumoniae and Azospirillum halopraeferens on the growth and development of two Salicornia bigelovii...
Genome sequence of Shigella flexneri strain SP1, a diarrheal isolate that encodes an extended-spectrum β-lactamase ESBL - pdf...
cbbS - Ribulose bisphosphate carboxylase small chain (RuBisCO small subunit) - Azospirillum lipoferum (strain 4B) - cbbS gene &...
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Roseomonas
However, further studies found that R. fauriae was a strain of Azospirillum brasilense, and did not belong in the genus ... 1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. International Journal of Systematic and ...
GUS reporter system
"Bacterial chemotactic motility is important for the initiation of wheat root colonization by Azospirillum brasilense". ...
Streptomyces mutabilis
El-Shanshoury, A. R. (September 1995). "Interactions of Azotobacter chroococcum, Azospirillum brasilense and Streptomyces ...
Balaram Mukhopadhyay
Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the ... "Synthesis of the tetrasaccharide related to the repeating unit of the O-antigen from Azospirillum brasilense Jm125A2 in the ...
Vicia
When the root nodules of broad bean are inoculated with the rhodospirillacean bacterium Azospirillum brasilense and the ...
List of MeSH codes (B03)
Azospirillum MeSH B03.440.400.425.708.100.020 - Azospirillum brasilense MeSH B03.440.400.425.708.100.510 - Azospirillum ... Azospirillum MeSH B03.660.050.663.750.100.020 - Azospirillum brasilense MeSH B03.660.050.663.750.100.510 - Azospirillum ...
Desert
... which exposed the roots of different species of tree to rhizobacteria and the nitrogen fixing bacterium Azospirillum brasilense ...
Azospirillum
... gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov". ... who also added Azospirillum brasilense to the genus. By 2020, twenty-one species of Azospirillum had been described, most of ... "Root-surface colonization of black mangrove seedlings by Azospirillum halopraeferens and Azospirillum brasilense in seawater". ... Azospirillum are gram-negative, do not form spores, and have a slightly-twisted oblong-rod shape. Azospirillum have at least ...
Azospirillum canadense
"Variabilidad genética de aislamientos no-típicos de Azospirillum brasilense por análisis PCR-RFLP del ADN 16S ribosomal." ... "Azospirillum canadense" at the Encyclopedia of Life LPSN Type strain of Azospirillum canadense at BacDive - the Bacterial ... Azospirillum canadense is a nitrogen-fixing bacterium isolated from corn rhizospheres. Its type strain is DS2T (=NCCB 100108T = ... LMG 23617T). Mehnaz, S.; Weselowski, B.; Lazarovits, G. (2007). "Azospirillum canadense sp. nov., a nitrogen-fixing bacterium ...
Azospirillum halopraeferens
"Root-surface colonization of black mangrove seedlings by Azospirillum halopraeferens and Azospirillum brasilense in seawater". ... "Azospirillum halopraeferens" at the Encyclopedia of Life LPSN Type strain of Azospirillum halopraeferens at BacDive - the ... Azospirillum halopraeferens is a species of nitrogen-fixing bacteria associated with the roots of Diplachne fusca and black ... Reinhold, B.; Hurek, T.; Fendrik, I.; Pot, B.; Gillis, M.; Kersters, K.; Thielemans, S.; De Ley, J. (1987). "Azospirillum ...
Azospirillum brasilense
"Azospirillum brasilense" at the Encyclopedia of Life Type strain of Azospirillum brasilense at BacDive - the Bacterial ... Azospirillum gen. nov. and two species, Azospirillum lipoferum(Beijerinck) comb. nov. and Azospirillum brasilense sp. nov". ... Azospirillum brasilense is a well studied, nitrogen-fixing (diazotroph), genetically tractable, Gram-negative, alpha- ... Tien TM, Gaskins MH, Hubbell DH (May 1979). "Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on ...
KEGG PATHWAY: Peptidoglycan biosynthesis - Azospirillum brasilense Sp245
View source for Azospirillum brasilense - microbewiki
Azospirillum brasilense=== ==Description and Significance== Azospirillum brasilense is one of the most well-studied ... View source for Azospirillum brasilense. From MicrobeWiki, the student-edited microbiology resource ... References , [2]]] A.brasilense is capable of promoting growth of plants through the secretion of phytohormones, the most ... A.brasilense colonizes the surface of plant roots through a two step process: attachment and anchoring. [[#References , [1 ...
Plasmid Rearrangements and Changes in Cell-Surface Architecture and Social Behavior of Azospirillum brasilense | SpringerLink
Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can ... Bastarrachea F, Zamudio M, Rivas R (1988) Non-encapsulated mutants of Azospirillum brasilense and Azospirillum lipoferum. Can J ... Azospirillum brasilense SR75 O-specific polysaccharide and homology of the lps loci in the plasmids of Azospirillum brasilense ... Genome sequence of Azospirillum brasilense CBG497 and comparative analyses of Azospirillum core and accessory genomes provide ...
Effects of Azospirillum brasilense and Pseudomonas fluorescens on nitrogen transformation and enzyme activity in the rice...
... brasilense) and Pseudomonas fluorescens ( P. fluorescens) have long been known to benefit inoculated rice plants, but their ... Azospirillum brasilense (A. brasilense) and Pseudomonas fluorescens (P. fluorescens) have long been known to benefit inoculated ... Effects of Azospirillum brasilense and Pseudomonas fluorescens on nitrogen transformation and enzyme activity in the rice ... brasilense (Mb), rice seedling roots inoculated with P. fluorescens (Mp), and rice seedling roots inoculated with a mixture of ...
Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7. - PubMed - NCBI
A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In ... Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7.. Potrich DP1, Bressel ... This result suggests the differential expression of the nif genes and their respective products in Azospirillum. ...
Search results for: 'Azospirillum brasilense Sp245'
Aerobic Nitric Oxide Production by Azospirillum brasilense Sp245 and Its Influence on Root Architecture in Tomato
The major feature of the plant-growth-promoting bacteria Azospirillum brasilense is its ability to modify plant root ... These results show that aerobic NO synthesis in A. brasilense could be achieved by different pathways and give evidence for an ... Here, we analyzed aerobic NO production by A. brasilense Sp245 wild type (wt) and its mutants Faj009 (IAA-attenuated) and ... providing evidence that NO mediated Azospirillum-induced root branching. ...
African Journal of Microbiology Research - mutants of the hybrid sensor kinase csta affect cyst cell development in...
Key words: Two-component system protein, ·cyst development, ·nitrogen fixation,·Azospirillum brasilense. ... Moreover, cstA mutants are affected in colony morphology which are essential for A. brasilense cells to differentiate into cyst ... These observations suggested that cstA is involved in cyst development in A. brasilense. ... Mutants of the hybrid sensor kinase CstA affect cyst cell development in Azospirillum brasilense Sp7. Lixian Wu 1#, Yanhua Cui ...
African Journal of Agricultural Research - effects of associated co-inoculation of bradyrhizobium japonicum with azospirillum...
with Azospirillum brasilense is beneficial to soybean yield, mainly using the in-furrow method of application for ... In this context, Azospirillum species, a nitrogen-fixing microorganism found in the rhizosphere of various plant species, may ... in-furrow and seed-applied) combined with an in-furrow application of Azospirillum spp. At R2 growth stage, the nodule number, ... associated with the co-inoculation with Azospirillum spp. The tested treatments consisted of the absence of N fertilizer and ...
Characterization of an Azospirillum brasilense Tn5 Mutant with Enhanced Nitrogen Fixtation | Springer for Research & Development
EMDB-10104: Structure of Azospirillum brasilense Glutamate Synthase in a4b4 o... - Yorodumi
Azospirillum brasilense (bacteria). Method. single particle reconstruction / cryo EM / Resolution: 3.9 Å Details. Authors. ... Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.. Map data. Details. Sample. Glutamate ... Title: Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.. Authors: Paolo Swuec / ... Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states ( ...
Metabolic Adaptations of Azospirillum brasilense to Oxygen Stress by Cell-to-Cell Clumping and Flocculation | Applied and...
Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation. J Bacteriol 163:716- ... Azospirillum lipoferum and Azospirillum brasilense surface polysaccharide mutants that are affected in flocculation. J Appl ... Calcofluor- and lectin-binding exocellular polysaccharides of Azospirillum brasilense and Azospirillum lipoferum. J Bacteriol ... An Azospirillum brasilense Tn5 mutant with modified stress response and impaired in flocculation. Antonie Van Leeuwenhoek 83:35 ...
Carbon Sources for the In-Vivo Nitrate Reductase Activity in Azospirillum Brasilense Cells | Springer for Research & Development
Sadasivan L., Neyra C.A. (1988) Carbon Sources for the In-Vivo Nitrate Reductase Activity in Azospirillum Brasilense Cells. In ... Carbon Sources for the In-Vivo Nitrate Reductase Activity in Azospirillum Brasilense Cells. ... Nitrate reduction nitrate reductase activity carbon metabolism A. brasilense This is a preview of subscription content, log in ... The use of α -KG as energy source by the A brasilense cells was confirmed by the uptake studies using radioactively labelled C ...
Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting...
Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense. Yoav Bashan, Luz E. de-Bashan ... tomato) in tomatoes treated with a combination of Azospirillum brasilense, bactericides and mild heat, p. 296-300. In S. H. De ... Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense Message Subject (Your Name) has forwarded a page ... Tomato plants are hosts to Azospirillum brasilense, a plant growth-promoting bacterium (PGPB) (7, 8), and Pseudomonas syringae ...
The Use of Fragments of the 85- and 120-MDa Plasmids of Azospirillum brasilense Sp245 to Study the Plasmid Rearrangement in...
Study the Plasmid Rearrangement in This Bacterium and to Search for Homologous Sequences in Plasmids of Azospirillum brasilense ... and 120-MDa Plasmids of Azospirillum brasilense Sp245 to ... and Azospirillum brasilense sp. Nov.. Tarrand, J.X.; Krieg, N.E ... The Use of Fragments of the 85- and 120-MDa Plasmids of Azospirillum brasilense Sp245 to Study... Katsy, E.; Borisov, I.; ... The Use of Fragments of the 85- and 120-MDa Plasmids of Azospirillum brasilense Sp245 to Study the Plasmid Rearrangement in ...
GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli. - Semantic Scholar
Our results show that the GlnB protein is required for transcription activation by Azospirillum brasilense NifA and it cannot ... The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were ... Functional analysis of the fixL/fixJ and fixK genes in Azospirillum brasilense Sp7. *Huamin Li, Feng Xu, Xiaojie Ren, Sanfeng ... Expression and characterization of an N-truncated form of the NifA protein of Azospirillum brasilense. *C.Y. Nishikawa, L M ...
Azospirillum brasilense - Wikipedia
"Azospirillum brasilense" at the Encyclopedia of Life Type strain of Azospirillum brasilense at BacDive - the Bacterial ... Azospirillum gen. nov. and two species, Azospirillum lipoferum(Beijerinck) comb. nov. and Azospirillum brasilense sp. nov". ... Azospirillum brasilense is a well studied, nitrogen-fixing (diazotroph), genetically tractable, Gram-negative, alpha- ... Tien TM, Gaskins MH, Hubbell DH (May 1979). "Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on ...
A constitutively expressed pair of rpoE2-chrR2 in Azospirillum brasilense Sp7 is required for survival under antibiotic and...
The Azospirillum brasilense Sp245 genome harbours nine genes encoding σE, of which two are adjacent to the genes encoding ChrR- ... We describe here the role and regulation of a new pair of rpoE-chrR, which was found in the genome of A. brasilense Sp7 in ... Inactivation of chrR2 or overexpression of rpoE2 in A. brasilense Sp7 resulted in an overproduction of carotenoids. It also ... Vanstockem M., Michiels K., Vanderleyden J., Van Gool A. P..( 1987;). Transposon mutagenesis of Azospirillum brasilense and ...
Azospirillum brasilense: Laboratory Maintenance and Genetic Manipulation - Current Protocols
... including the most comprehensively studied Azospirillum brasilense, are non‐pathogenic soil bacteria that promote the growth of ... Bacteria of the genus Azospirillum, including the most comprehensively studied Azospirillum brasilense, are non‐pathogenic soil ... Genome sequence of Azospirillum brasilense CBG497 and comparative analyses of Azospirillum core and accessory genomes provide ... Azospirillum brasilense: Laboratory Maintenance and Genetic Manipulation. Jessica Gullett1, Lindsey ONeal1, Tanmoy Mukherjee1 ...
Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in...
The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and ... To understand the role of A. brasilense Sp7 PhaP1 (PhaP1Abs) on PHB synthesis, the phaP1 gene (AMK58_RS17065) was deleted. The ... This study reports the first analysis of a phasin protein in A. brasilense Sp7. ... brasilense. phaP1, phaP2 and phaP3 genes were able to respond to the growth conditions tested. ...
Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient...
Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by A. brasilense is a good model to ... We performed a dual RNA-Seq transcriptional profiling of wheat roots colonized by A. brasilense strain FP2. cDNA libraries from ... Overall, we identified 23,215 wheat expressed ESTs and 702 A. brasilense expressed transcripts. Bacterial colonization caused ... brasilense expressed genes. PGPB colonization enhanced the expression of plant genes related to nutrient up-take, nitrogen ...
Domain assignment for gi|392384140|ref|YP 005033336.1| from Azospirillum brasilense Sp245
Nutritional aspects of corn due to cover crops, nitrogen doses and inoculation with Azospirillum brasilense
... Author. * Portugal ... Nutritional aspects of corn due to cover crops, nitrogen doses and inoculation with Azospirillum brasilense. Australian Journal ... Inoculation via seeds using Azospirillum brasilense did not increase the accumulation of dry matter and nutrients of corn ... in topdressing and inoculation via seeds using Azospirillum brasilense. The experiment comprised randomized blocks in a 6 × 4 ...
Entwicklung von Methoden zur Identifizierung, Lokalisierung und Quantifizierung von Azospirillum-brasilense-Stämmen mit...
Entwicklung von Methoden zur Identifizierung, Lokalisierung und Quantifizierung von Azospirillum-brasilense-Stämmen mit ... Azospirillum brasilense; Immunoassay; Monoklonaler Antikörper. Institutions of the University:. Faculties , Faculty of Biology ... Entwicklung von Methoden zur Identifizierung, Lokalisierung und Quantifizierung von Azospirillum-brasilense-Stämmen mit ...
Plants | Free Full-Text | Molecular Analyses of the Distribution and Function of Diazotrophic Rhizobia and Methanotrophs in...
Azospirillum brasilense. Bradyrhizobium spp. Methylocapsa spp. Paraburkholderia tropica. Idenella/Herbaspirillum-like. White ... in the leaf sheath and Azospirillum brasilense, Bradyrhizobium spp., Methylocapsa spp., Paraburkholderia tropica, and Idenella/ ... Azospirillum, and Klebsiella in the root; and Bradyrhizobium, Ideonella, Azospirillum, and Klebsiella in the rhizosphere soil ( ... Azospirillum. Klebsiella. Herbaspirillum. Raoultella. Methylosinus, Rhizobium. Root harvested in six regions in Rio Grande do ...
Biological Nitrogen Fixation, 2 Volume Set | Molecular Microbiology | Microbiology & Virology | Life Sciences | Subjects | Wiley
Marine Drugs | Free Full-Text | Novel Conopeptides of Largely Unexplored Indo Pacific Conus sp. | HTML
Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - Azospirillum...
The seeds treated with 75% Chemical fertilizer + Azospirillum brasilense + Pseudomonas fluorescens (T6) showed maximum plant ... Based upon their N-fixing ability and IAA production, two strains, one Azospirillum sp. and one Pseudomonas sp. isolate was ... An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from ... An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from ...
Caracterização de uma fosforribohidrolase do tipo lonely guy de Azospirillum brasilense, com uma possível ação na biossíntese...
Azospirillum brasilense FP2 has two genes homologous to LOG of A. thaliana as revealed by genome searching through ... Palavra-chave: Citocininas, Azospirillum brasilense, fosforribohidrolases.. Abstract: The LONELY GUY gene (LOG) of Arabidopsis ... Caracterização de uma fosforribohidrolase do tipo lonely guy de Azospirillum brasilense, com uma possível ação na biossíntese ... A. brasilense é uma bactéria associativa de plantas, fixadora de nitrogênio e promotora de crescimento vegetal. A produção de ...
Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense | Braz. j. med...
NifA of Azospirillum brasilense was purified in the form of a glutathione-S-transferase (GST)-NifA fusion protein and ... Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Carrier Proteins / Azospirillum brasilense / ... Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Carrier Proteins / Azospirillum brasilense / ... Azospirillum brasilense/genetics , Bacterial Proteins/isolation & purification , Carrier Proteins , Nitrogen Fixation , ...
Sp245Promoting bacteriaLipoferumApplied and EnvironmBacteriumGenesPseudomonasBacterialAzotobacterBradyrhizobiumStrainsGENOMERhizosphereNifAProteinGenusTarrandNitrogenaseIndole-3-acSpeciesRoots2000MaizeInoculantsRhizobacteriumFixationPhysiologicalSeropedicaeUptakeEnzymeRhodospirillaceaeMetabolismPlasmidPhytohormonesPolar flagellumIsolateNitrogen dosesMotileGeneticsGrowth and yieldFlagellumSynthesis1983ProteobacteriaATCCMutantsAminoYieldMolecular1988EnterobacterMicroorganismsSoilPlantGenePromoter
Sp2457
- The genome of A. brasilense Sp245 has been sequenced and is 7Mbp in size and spread across 7 chromosomes. (wikipedia.org)
- In this chapter, experimental data on plasmid plasticity in the type strain A. brasilense Sp7 and in the facultative endophyte A. brasilense Sp245 are discussed. (springer.com)
- A spontaneous reorganization of 85- and 120-MDa plasmids gave rise to an A. brasilense Sp245 derivative with a novel cell-surface architecture and with altered social motility and biofilm formation. (springer.com)
- Here, we analyzed aerobic NO production by A. brasilense Sp245 wild type (wt) and its mutants Faj009 (IAA-attenuated) and Faj164 (periplasmic nitrate reductase negative), and its correlation with tomato root-growth-promoting effects. (apsnet.org)
- The Use of Fragments of the 85- and 120-MDa Plasmids of Azospirillum brasilense Sp245 to Study. (deepdyve.com)
- Two EcoRI fragments homologous to the fla/laf/mot/swa loci of p85 were found in A. brasilense Sp245 DNA, whereas only one copy was preserved in the Sp245.5 mutant. (deepdyve.com)
- The Azospirillum brasilense Sp245 genome harbours nine genes encoding σ E , of which two are adjacent to the genes encoding ChrR-type zinc-binding anti-sigma (ZAS) factors. (microbiologyresearch.org)
Promoting bacteria3
- 3]]] ==='''''Azospirillum brasilense'''''=== ==Description and Significance== ''Azospirillum brasilense'' is one of the most well-studied plant growth promoting bacteria. (kenyon.edu)
- 1]]] Many countries use bacterial inoculants containing ''A.brasilense'' alone or in concert with other plant growth promoting bacteria. (kenyon.edu)
- The major feature of the plant-growth-promoting bacteria Azospirillum brasilense is its ability to modify plant root architecture. (apsnet.org)
Lipoferum10
- A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum(Beijerinck) comb. (wikipedia.org)
- Bastarrachea F, Zamudio M, Rivas R (1988) Non-encapsulated mutants of Azospirillum brasilense and Azospirillum lipoferum . (springer.com)
- Due to this discovery, Spirillum lipoferum was reclassified in 1978 as Azospirillum lipoferum by Jeffery Tarrand, Noel Krieg, and Döbereiner, who also added Azospirillum brasilense to the genus. (wikipedia.org)
- Colonization of wheat roots with Azospirillum lipoferum labeled with the green fluorescent protein (gfp) reporter gene. (asmblog.org)
- To gain insights into how these bacteria transitioned from marine to terrestrial ecosystems, the authors sequenced the genomes of Azospirillum brasilense and Azospirillum lipoferum . (asmblog.org)
- Kinetics of kelthane uptake and distribution in azospirillum lipoferum. (worldcat.org)
- In A. brasilense and Azospirillum lipoferum posttranslational control of nitrogenase, in response to ammonium and anaerobiosis, involves ADP-ribosylation of the nitrogenase iron protein, mediated by the enzymes DraT and DraG. (agriville.com)
- Strain 21, Z 2 = Azospirillum lipoferum DSM 1691, Z 3 = Azospirillum brasilense DSM 1690 and Azotobacter strains were: A 0 = no inoculation, A 1 = Azotobacter sp. (scialert.net)
- The bacterial strains studied were Azospirillum lipoferum s-21 and Azotobacter chroococcum s-5 with known positive effects on wheat (Mahour, 2005) and canola (Khalilian, 2006). (scialert.net)
- Azospirillum lipoferum DSM 1691, Azospirillum brasilense DSM 1690 and Azotobacter chroococcum DSM 2286 were used as reference strains. (scialert.net)
Applied and Environm1
- Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on the Growth of Pearl Millet (Pennisetum americanum L.)". Applied and Environmental Microbiology. (wikipedia.org)
Bacterium9
- Bashan Y, de-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth-a critical assessment. (springer.com)
- The motile microaerophilic bacterium Azospirillum brasilense navigates oxygen gradients by aerotaxis in order to locate low oxygen concentrations that can support metabolism. (asm.org)
- tomato, the causal agent of bacterial speck of tomato, and the plant growth-promoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. (asm.org)
- Tomato plants are hosts to Azospirillum brasilense , a plant growth-promoting bacterium (PGPB) ( 7 , 8 ), and Pseudomonas syringae pv. (asm.org)
- Although A. brasilense is known as a rhizosphere bacterium ( 7 ), some strains are ephiphytic ( 7 , 10 ), and P. syringae pv. (asm.org)
- The plant growth promoting bacterium Azospirillum brasilense Sp7 accumulates high quantities of bioplastic PHB as carbon and energy source. (springeropen.com)
- A. brasilense is a plant associate bacterium, nitrogen-fixing and plant growth promoter. (ufpr.br)
- Increased growth of the microalga Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth-promoting bacterium Azospirillum brasilense. (bio.net)
- AZOS, short for Azospirillum Brasilense, is a nitrogen-fixing super bacterium that can replace 50% of the nitrogen fertilizer required by plants. (hydroponics.net)
Genes7
- A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. (nih.gov)
- This result suggests the differential expression of the nif genes and their respective products in Azospirillum. (nih.gov)
- In addition, genes encoding proteins related to bacterial chemotaxi, biofilm formation and nitrogen fixation were highly expressed in the sub-set of A. brasilense expressed genes. (biomedcentral.com)
- Por meio de ferramentas de bioinformática foram encontrados dois genes homólogos a LOG de Arabidopsis thaliana no genoma de Azospirillum brasilense FP2. (ufpr.br)
- Azospirillum brasilense FP2 has two genes homologous to LOG of A. thaliana as revealed by genome searching through computational analysis with amino acid sequences of LOGs previously described. (ufpr.br)
- The astonishing results indicated that about half of the Azospirillum genes seem to be horizontally transferred. (asmblog.org)
- A number of genes essential for Trp production have been isolated in A. brasilense, including trpE(G) which codes for anthranilate synthase, the key enzyme in Trp biosynthesis. (agriville.com)
Pseudomonas6
- Azospirillum brasilense ( A . brasilense ) and Pseudomonas fluorescens ( P. fluorescens ) have long been known to benefit inoculated rice plants, but their effects on nitrogen (N) transformations and enzyme activities in the rice rhizosphere are unclear. (springer.com)
- An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). (scipress.com)
- The seeds treated with 75% Chemical fertilizer + Azospirillum brasilense + Pseudomonas fluorescens (T 6 ) showed maximum plant vegetative characters, followed by others compared with control. (scipress.com)
- Bacterial strains - Bacillus cereus , Pseudomonas fluorescens , B. megaterium , Azospirillum brasilense , B. thuringiensis var. (scielo.br)
- PGPR within genera known to stimulate the growth of plants are Azospirillum, Azotobacter, Bacillus, Enterobacter, and Pseudomonas . (hindawi.com)
- Different plant growth-promoting rhizobacteria, including free-living and associative bacteria such as Azospirillum , Azotobacter , Bacillus and Pseudomonas have been used in agricultural systems as biofertilizer for their beneficial effects on plant growth (Tilak et al . (scialert.net)
Bacterial7
- 3]]] The phenotypic differentiation of ''A.brasilense'' from other diazotrophic members of group IV is based upon the size and shape of the bacterial cell, and the mode of nitrogen fixation among other things. (kenyon.edu)
- These results show that aerobic NO synthesis in A. brasilense could be achieved by different pathways and give evidence for an NO-dependent promoting activity on tomato root branching regardless of bacterial capacity for IAA synthesis. (apsnet.org)
- Inoculation of seeds with a mixed culture of the two bacterial strains resulted in a reduction of the pathogen population in the rhizosphere, an increase in the A. brasilense population, the prevention of bacterial speck disease development, and improved plant growth. (asm.org)
- tomato was established on the leaves did not alleviate bacterial speck disease, and A. brasilense did not survive well in the phyllosphere under these conditions, even in a mist chamber. (asm.org)
- Based on our results, we propose that A. brasilense be used in prevention programs to combat the foliar bacterial speck disease caused by P. syringae pv. (asm.org)
- Azospirillum is a Gram-negative, microaerophilic, non-fermentative and nitrogen-fixing bacterial genus from the family of Rhodospirillaceae. (wikipedia.org)
- Bacterial inoculation of maize, mainly with Azospirillum , has been a successful common practice (5, 23, 30) . (scielo.org.ar)
Azotobacter2
- Interactions of Azotobacter chroococcum, Azospirillum brasilense and Streptomyces mutabilis, in relation to their effect on wheat development. (thefreedictionary.com)
- The effect of plant growth-promoting rhizobacteria (PGPR) belonging to the genera Azospirillum and Azotobacter on the growth and nutrient uptake of maize ( Zea mays ) was investigated in the field as factorial experiment. (scialert.net)
Bradyrhizobium2
- with Azospirillum brasilense is beneficial to soybean yield, mainly using the in-furrow method of application for Bradyrhizobium spp. (academicjournals.org)
- Experimental treatments were 1) no inoculation 2) Azospirillum brasilense inoculate 3) Bradyrhizobium japonicum inoculate and 4) Rhizobium leguminosarum biovar viceae inoculate. (wku.edu)
Strains4
- To explore the effects of A. brasilense and P. fluorescens strains on N transformations and enzyme activities in the rice rhizosphere, N fertilizer was not applied in this trial, while the full doses of P (150 mg P 2 O 5 per pot) and K (320 mg K 2 O per pot) were applied. (springer.com)
- Specifically, in the case of A. brasilense, this characteristic could be used to generate more efficient strains in the process of promoting plant growth. (ufpr.br)
- Since the 1970s, Azospirillum strains have been researched for their effects in improving agricultural yields and improving growth of wild plants. (wikipedia.org)
- Azospirillum strains were: Z 0 = no inoculation, Z 1 = Azospirillum sp. (scialert.net)
GENOME4
- Whole-Genome Sequences of Four Indian Isolates of Azospirillum brasilense. (nih.gov)
- A third genome from an unidentified Azospirillum species was described while their work was in progress. (asmblog.org)
- In all three species, the Azospirillum genome consists of seven replicons. (asmblog.org)
- In fact, there are more genomic rearrangement in Azospirillum than in Rhizobium , the multi-replicon α-proteobacterium that previously had the distinction of having the most plastic genome known among the prokaryotes. (asmblog.org)
Rhizosphere11
- 1]]] ==Ecology and Pathogenesis== ''Azosprillum brasilense'' is routinely found in the plant rhizosphere of crop plants and agricultural lands as well as from grasses and cereals. (kenyon.edu)
- Assmus B, Hutzler P, Kirchhof G et al (1995) In situ localization of Azospirillum brasilense in the rhizosphere of wheat with fluorescently labeled, rRNA-targeted oligonucleotide probes and scanning confocal laser microscopy. (springer.com)
- This study explored whether A . brasilense and P. fluorescens are involved in modifying N transformations, N-supplying capacity, and enzyme activities in the rice rhizosphere, and the performance of rice biomass after inoculation with A . brasilense and P. fluorescens was also evaluated. (springer.com)
- Inoculating the rice rhizosphere with A . brasilense and P. fluorescens greatly improved the ammonification activities in the soil, and the effects were more favorable in the M bp treatment. (springer.com)
- Co-inoculation with A . brasilense and P. fluorescens in the rice rhizosphere greatly increased the nitrogenase activities in the soil, and inoculating with A . brasilense alone in the rice rhizosphere also showed good results. (springer.com)
- Finally, rice biomass increased greatly after A . brasilense and P. fluorescens inoculation in the rhizosphere. (springer.com)
- Inoculating A . brasilense and P. fluorescens in the rice rhizosphere accelerated N transformations and improved the N-supplying capacity of the rhizosphere soil, and increased rice biomass. (springer.com)
- The most beneficial effects were observed with A . brasilense and P. fluorescens co-inoculation in the rice rhizosphere. (springer.com)
- In this context, Azospirillum species, a nitrogen-fixing microorganism found in the rhizosphere of various plant species, may be studied as a way of providing soybean crop with the part of the N it needs. (academicjournals.org)
- When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. (asm.org)
- tomato did not survive in the rhizosphere in the presence of A. brasilense . (asm.org)
NifA9
- We previously characterized a gene (originally named org35 , renamed cstA ) encoding aPAS/PAC sensor hybrid histidine kinase (HK) which interacts with Azospirilum brasilense NifA in a yeast two-hybrid system. (academicjournals.org)
- GlnB is specifically required for Azospirillum brasilense NifA activity in Escherichia coli. (semanticscholar.org)
- The Azospirillum brasilense transcription regulator NifA and the nitrogen-status signaling proteins GlnB, GlnZ and GlnK were expressed in Escherichia coli and analyzed for their ability to activate nif gene expression. (semanticscholar.org)
- NifA of Azospirillum brasilense was purified in the form of a glutathione -S- transferase (GST)-NifA fusion protein and proteolytic release of GST yielded inactive and partially soluble NifA. (bvsalud.org)
- However, the purified NifA was able to induce the production of specific anti-A. brasilense NifA-antiserum that recognized NifA from A. brasilense but not from K. pneumoniae. (bvsalud.org)
- Both GST-NifA and NifA expressed from the E. coli tac promoter are able to activate transcription from the nifHDK promoter but only in an A. brasilense background. (bvsalud.org)
- In order to investigate the mechanism that regulates NifA binding capacity we have used E. coli total protein extracts expressing A. brasilense nifA in mobility shift assays . (bvsalud.org)
- These data show that the binding activity present in the C-terminal domain of A. brasilense NifA protein is still functional even in the presence of oxygen . (bvsalud.org)
- In Azospirillum brasilense and H. seropedicae (alpha- and beta-subgroup, respectively), NifA is inactive in conditions of excess nitrogen. (agriville.com)
Protein4
- This study reports the first analysis of a phasin protein in A. brasilense Sp7. (springeropen.com)
- In this study, purified ADP‐ribosylated Fe‐protein was used as substrate to study the mechanism involved in the regulation of A. brasilense DraG in vitro. (usda.gov)
- Structural and functional analysis of Azospirillum brasilense GlnB protein. (postjobfree.com)
- I have been working with chemotactic protein expression in the rhizobacterium Azospirillum brasilense under various growth conditions using mRNA as evidence of protein presence. (bioquest.org)
Genus5
- An isolate from the genus Azospirillum was isolated from nitrogen poor soils in the Netherlands in 1925, however the species A. brasilense was first described in 1978 in Brazil, since this genus is widely found in the rhizospheres of grasses around the world where it confers plant growth promotion. (wikipedia.org)
- The genus Azospirillum belongs in the alpha-Proteobacteria class of bacteria. (wikipedia.org)
- The genus Azospirillum belongs to the α-proteobacteria, one of the major groups of hydrobacteria (a clade of prokaryotes that first appeared in marine habitats). (asmblog.org)
- Based on the phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY788 T represents a novel species of the genus Azospirillum , for which the name Azospirillum soli sp. (microbiologyresearch.org)
- Azospirillum represents the best characterized genus of plant growth-promoting rhizobacteria. (agriville.com)
Tarrand2
- 1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. (wikipedia.org)
- Azospirillum Tarrand, Krieg and Dobereiner, 79AL. (microbiologyresearch.org)
Nitrogenase3
- 1]]] ''A.brasilense'' is capable of fixing nitrogen through assimilation of ammonium and the activity of nitrogenase. (kenyon.edu)
- How Does the DraG-P II Complex Regulate Nitrogenase Activity in Azospirillum brasilense ? (wiley.com)
- The ammonium‐dependent posttranslational regulation of nitrogenase activity in Azospirillum brasilense requires dinitrogenase reductase ADP‐ribosyl transferase (DraT) and dinitrogenase reductase ADP‐glycohydrolase (DraG). (usda.gov)
Indole-3-ac2
- For example, Azospirillum colonizes the roots of cereals (rice, wheat, and maize) and other grasses where it enhances plant growth by carrying out nitrogen fixation and secreting phytohormones, such as indole-3-acetic acid. (asmblog.org)
- At least three pathways for indole-3-acetic acid (IAA) biosynthesis in A. brasilense exist: two Trp-dependent (the indole-3-pyruvic acid and presumably the indole-3-acetamide pathway) and one Trp-independent pathway. (agriville.com)
Species4
- 3]]] ''A.brasilense'' can be distinguished from other Azosprillum species based upon the ability of utilization of ribose and mannose. (kenyon.edu)
- By 2020, twenty-one species of Azospirillum had been described, most of which had been discovered after the year 2000. (wikipedia.org)
- Over 113 species of plants in 35 different plant families have been documented to have benefited from association with a species of Azospirillum. (wikipedia.org)
- Azospirillum brasilense , and several species of Paenibacillus ( P. fujiensis , P. durus , P. borealis , P. graminis , P. massiliensis and P. wynnii ) were identified. (scirp.org)
Roots14
- A.brasilense'' colonizes the surface of plant roots through a two step process: attachment and anchoring. (kenyon.edu)
- Spontaneous changes in the plasmid profile of A. brasilense Sp7 derivatives encompassing 90- and 115-MDa replicons were concurrent with variations in the colony morphology, cell antigenic structure, swarming, and biofilm formation on abiotic surfaces and plant roots. (springer.com)
- Burdman S, Jurkevitch E, Okon Y (2000) Surface characteristics of Azospirillum brasilense in relation to cell aggregation and attachment to plant roots. (springer.com)
- Croes CL, Moens S, van Bastelaere E et al (1993) The polar flagellum mediates Azospirillum brasilense adsorption to wheat roots. (springer.com)
- Rice inoculation was conducted under pot culture conditions in 2014 and 2015, and the experiment included four treatments: a control (CT), rice seedling roots inoculated with A . brasilense (M b ), rice seedling roots inoculated with P. fluorescens (M p ), and rice seedling roots inoculated with a mixture of A . brasilense and P. fluorescens (M bp ). (springer.com)
- Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by A. brasilense is a good model to investigate the molecular basis of plant-PGPB interaction including improvement in plant-NUE promoted by PGPB. (biomedcentral.com)
- We performed a dual RNA-Seq transcriptional profiling of wheat roots colonized by A. brasilense strain FP2. (biomedcentral.com)
- cDNA libraries from biological replicates of colonized and non-inoculated wheat roots were sequenced and mapped to wheat and A. brasilense reference sequences. (biomedcentral.com)
- Azospirillum are found in freshwater and soil habitats, especially in close relationships with plant roots. (wikipedia.org)
- Many Azospirillum excrete plant hormones that alter how the roots of plants grow. (wikipedia.org)
- Azospirillum also make antioxidants that protect the plant roots from stresses due to drought and flooding. (wikipedia.org)
- Azospirillum competes with pathogens on the roots for space and for trace nutrients such as iron. (wikipedia.org)
- The attachment of Azospirillum cells to plant roots occurs in two steps. (agriville.com)
- 2001). Roots of Azospirillum -inoculated maize seedlings were found to have higher amounts of both free and bound IAA as compared to control. (scialert.net)
20001
- Blaha C.A.G., Revers L.F., Passaglia L.M.P., Frazzon J., Schrank I.S. (2000) Characterization of an Azospirillum brasilense Tn5 Mutant with Enhanced Nitrogen Fixtation. (springer.com)
Maize1
- Efficiency of inoculant with Azospirillum brasilense on the growth and yield of second-harvest maize. (academicjournals.org)
Inoculants1
- In 2009, the first commercial inoculants containing Azospirillum came on the market, and by 2018, over 3 million doses were applied annually to crops by farmers, mainly in South America. (wikipedia.org)
Rhizobacterium1
- RpoH2 sigma factor controls the photooxidative stress response in a non-photosynthetic rhizobacterium, Azospirillum brasilense Sp7. (semanticscholar.org)
Fixation2
- However, how much nitrogen Azospirillum contribute to crop plants via biological fixation is debated. (wikipedia.org)
- Four aspects of the Azospirillum-plant root interaction are highlighted: natural habitat, plant root interaction, nitrogen fixation and biosynthesis of plant growth hormones. (agriville.com)
Physiological1
- Cohen MF, Han XY, Mazzola M (2004) Molecular and physiological comparison of Azospirillum spp. (springer.com)
Seropedicae1
- Effect of nitrogen fertilization associated with inoculation of Azospirillum brasilense and Herbaspirillum seropedicae on corn. (academicjournals.org)
Uptake2
- The use of α -KG as energy source by the A brasilense cells was confirmed by the uptake studies using radioactively labelled C 14 α -KG. (springer.com)
- The study was undertaken to analyze the rate of uptake and utilization of various amino acids by Azospirillum brasilense Sp81 (RG) in a basal mineral salts solution under non- nitrogen fixing condition. (bvsalud.org)
Enzyme1
- 15835904 ) In addition, the enzyme was compared with the phenylpyruvate decarboxylase from Azospirillum brasilense and the indolepyruvate decarboxylase from Enterobacter cloacae. (hmdb.ca)
Rhodospirillaceae1
- Within its family, the Rhodospirillaceae, almost all of its known relatives are found in aquatic environments, suggesting that Azospirillum may have transitioned to life on land later, after the Precambrian divergence of hydrobacteria and terrabacteria. (asmblog.org)
Metabolism2
- 3]]] ==Structure, Metabolism, and Life Cycle== ''Azosprillium brasilense'' has a vibroid shape with single polar flagellum attached to the cell making it motile. (kenyon.edu)
- Azospirillum brasilense 's preferred metabolism is adapted to microaerophily, and these motile cells quickly navigate to zones of low oxygen concentration by aerotaxis. (asm.org)
Plasmid1
- Acosta-Cruz E, Wisniewski-Dyé F, Rouy Z et al (2012) Insights into the 1.59-Mbp largest plasmid of Azospirillum brasilense CBG497. (springer.com)
Phytohormones1
- 2]]] ''A.brasilense'' is capable of promoting growth of plants through the secretion of phytohormones, the most common being Indole Acetic Acid (IAA). (kenyon.edu)
Polar flagellum2
- Azospirillum brasilense is a motile alphaproteobacterium that possesses a single polar flagellum. (asm.org)
- Motile A. brasilense cells respond tactically not only by biasing the probability of changes in the swimming direction of the polar flagellum (changes in swimming reversal frequency, equivalent to "tumbles" of E. coli ) but also by modulating transient increases in swimming velocity ( 6 ) ( Fig. 1 ). (asm.org)
Isolate1
- Microbiologists use nitrogen-free semi-solid media to isolate Azospirillum from samples. (wikipedia.org)
Nitrogen doses1
- The objective of this study is to evaluate the accumulation potential of nitrogen, phosphorus and potassium in: a) cover crops (millet, crotalaria, pigeon pea, millet + crotalaria, millet + pigeon pea and fallow) and b) corn plants sown in succession to the cover crops and with application of nitrogen doses (0, 40, 80 and 120 kg ha-1) in topdressing and inoculation via seeds using Azospirillum brasilense. (unesp.br)
Motile1
- When cells are exposed to elevated levels of oxygen in their surroundings, motile A. brasilense cells implement an alternative response to aerotaxis and form transient clumps by cell-to-cell interactions. (asm.org)
Genetics1
- Azospirillum IV: Genetics, Physiology and Ecology, p.16-25. (springer.com)
Growth and yield1
- Whereas A. brasilense increases the growth and yield of tomato plants ( 14 ), P. syringae pv. (asm.org)
Flagellum1
- Azospirillum have at least one flagellum and sometimes multiple flagella, which they use to move rapidly. (wikipedia.org)
Synthesis1
- To understand the role of A. brasilense Sp7 PhaP1 (PhaP1 Abs ) on PHB synthesis, the phaP1 gene (AMK58_RS17065) was deleted. (springeropen.com)
19831
- Baldani VLD, Baldani JI, Döbereiner J (1983) Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. (springer.com)
Proteobacteria1
- A.brasilense'' belongs to the alpha subclass of Proteobacteria, Alphaproteobacteria. (kenyon.edu)
ATCC1
- fructose, gluconate, glucose and TCA cycle intermediates were used as energy sources to study the invivo nitrate reductase activity in whole cells of A brasilense ATCC 29145. (springer.com)
Mutants1
- Moreover, cstA mutants are affected in colony morphology which are essential for A. brasilense cells to differentiate into cyst-like forms. (academicjournals.org)
Amino2
- A.brasilense'' produces IAA by utilizing the amino acid tryptophan as a precursor. (kenyon.edu)
- Mode of utilization of amino acids as growth substrates by Azospirillum brasilense. (bvsalud.org)
Yield3
- obtained grain yield of 0.82 t ha 1 in wheat crop inoculated with Azopirillum brasilense and given 37.5 kg N ha 1 (75% of the recommended rate) compared with 0.78 with no seed inoculation. (scialert.net)
- Currently, the EEA C. del Uruguay is working on seed inoculation with Azospirillum which is presented as a promoter of root growth, the first results have given substantial increases in the rapeseed granary yield in the plots inoculated with azospirillum but said work it is still under evaluation. (agriville.com)
- 1985). Also, co-inoculation with Giomus mosseae and G. fasciculatum in the presence of Azospirillum brasilense produced significantly higher dry matter production and grain yield of barley than their corresponding controls (Suba Rao et al . (scialert.net)
Molecular1
- Adaptaci n Molecular en Cianobacterias: Interacci n entre el Metabolismo del Nitr geno y el Carbono con el Estado Redox Celular Via la Acci n de Metales Pesados. (us.es)
19881
- Sadasivan L., Neyra C.A. (1988) Carbon Sources for the In-Vivo Nitrate Reductase Activity in Azospirillum Brasilense Cells. (springer.com)
Enterobacter1
- En este trabajo se aisló una bacteria de la rizósfera de maíz, que fue caracterizada mediante métodos fenotípicos y genotípicos e identifcada como Enterobacter sp. (scielo.org.ar)
Microorganisms1
- Symbiotic (Rhizobium and Frankia) and nonsymbiotic microorganisms (Azospirillum) are known to improve the soil fertility by fixing the atmospheric nitrogen. (intechopen.com)
Soil1
- Soil redox potential in presence of azospirillum. (worldcat.org)
Plant8
- Azospirillum/Plant Associations. (kenyon.edu)
- Azospirillum bacteria can promote plant growth. (wikipedia.org)
- Azospirillum promote plant growth through a variety of mechanisms. (wikipedia.org)
- In addition to these changes, Azospirillum can also alter the forms of plant nutrients such as nitrogen and phosphorus to make them more available to plants. (wikipedia.org)
- Plant growth can also be promoted indirectly by Azospirillum reducing plant disease. (wikipedia.org)
- observed in green house trials that plant dry weight and grain weight per spike were higher in 60 percent of durum wheat cultivars inoculated with Azopirillum brasilense strain Sp 7 than uninoculated control. (scialert.net)
- The team's experiments showed that S. viridis received 100% of its nitrogen needs from the bacteria Azospirillum brasilense when associated with plant root surfaces. (isaaa.org)
- The relevance of each of these four aspects for plant growth promotion by Azospirillum is discussed. (agriville.com)
Gene1
- Nevertheless, the indole-3-pyruvate decarboxylase encoding ipdC gene is crucial in the overall IAA biosynthesis in Azospirillum. (agriville.com)
Promoter1
- Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7. (nih.gov)