Azoarcus: A genus of gram-negative, facultatively anaerobic bacteria including species which are often associated with grasses (POACEAE) and which fix nitrogen as well as species which anaerobically degrade toluene and other mono-aromatic hydrocarbons.Gram-Negative Facultatively Anaerobic Rods: A large group of facultatively anaerobic, rod-shaped bacteria that show up as pink (negative) when treated by the gram-staining method.Thauera: A genus of gram-negative, rod-shaped bacteria able to anaerobically oxidize and degrade toluene.Toluene: A widely used industrial solvent.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Nitrogen Compounds: Inorganic compounds that contain nitrogen as an integral part of the molecule.Xylenes: A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Hydrocarbons, Aromatic: Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.Poaceae: A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Benzoates: Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.Carbon-Carbon Lyases: Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Phenol: An antiseptic and disinfectant aromatic alcohol.RNA, Catalytic: RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.Endophytes: An endosymbiont that is either a bacterium or fungus living part of its life in a plant. Endophytes can benefit host plants by preventing pathogenic organisms from colonizing them.ResorcinolsDNA End-Joining Repair: The repair of DOUBLE-STRAND DNA BREAKS by rejoining the broken ends of DNA to each other directly.DNA Breaks, Double-Stranded: Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently.DNA Ligases: Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 6.5.1.1 (ATP) and EC 6.5.1.2 (NAD).Antigens, Nuclear: Immunologically detectable substances found in the CELL NUCLEUS.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.DNA-Activated Protein Kinase: A serine-threonine protein kinase that, when activated by DNA, phosphorylates several DNA-binding protein substrates including the TUMOR SUPPRESSOR PROTEIN P53 and a variety of TRANSCRIPTION FACTORS.Gram-Negative Facultatively Anaerobic Rods: A large group of facultatively anaerobic, rod-shaped bacteria that show up as pink (negative) when treated by the gram-staining method.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Papaveraceae: The poppy plant family of the order Papaverales, subclass Magnoliidae, class Magnoliopsida. These have bisexual, regular, cup-shaped flowers with one superior pistil and many stamens; 2 or 3 conspicuous, separate sepals and a number of separate petals. The fruit is a capsule. Leaves are usually deeply cut or divided into leaflets.Chemoreceptor Cells: Cells specialized to detect chemical substances and relay that information centrally in the nervous system. Chemoreceptor cells may monitor external stimuli, as in TASTE and OLFACTION, or internal stimuli, such as the concentrations of OXYGEN and CARBON DIOXIDE in the blood.Antirrhinum: A plant genus of the family Plantaginaceae. Members contain DEFICIENS PROTEIN.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Flowers: The reproductive organs of plants.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Peptide Termination Factors: Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.Computer Simulation: Computer-based representation of physical systems and phenomena such as chemical processes.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Physiological Phenomena: The functions and properties of living organisms, including both the physical and chemical factors and processes, supporting life in single- or multi-cell organisms from their origin through the progression of life.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Physiological Processes: The functions and activities of living organisms that support life in single- or multi-cellular organisms from their origin through the progression of life.Models, Theoretical: Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.Molecular Dynamics Simulation: A computer simulation developed to study the motion of molecules over a period of time.NIH 3T3 Cells: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)National Heart, Lung, and Blood Institute (U.S.): Component of the NATIONAL INSTITUTES OF HEALTH. It conducts and supports research program related to diseases of the heart, blood vessels, lung, and blood; blood resources; and SLEEP WAKE DISORDERS. From 1948 until October 10, 1969, it was known as the National Heart Institute. From June 25, 1976, it was the National Heart and Lung Institute. Since October 1997, the NHLBI has also had administrative responsibility for the NIH Woman's Health Initiative.Glycine Hydroxymethyltransferase: A pyridoxal phosphate enzyme that catalyzes the reaction of glycine and 5,10-methylene-tetrahydrofolate to form serine. It also catalyzes the reaction of glycine with acetaldehyde to form L-threonine. EC 2.1.2.1.Structural Homology, Protein: The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Computer Graphics: The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.Pantothenic Acid: A butyryl-beta-alanine that can also be viewed as pantoic acid complexed with BETA ALANINE. It is incorporated into COENZYME A and protects cells against peroxidative damage by increasing the level of GLUTATHIONE.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Hydroxymethyl and Formyl Transferases: Enzymes that catalyze the transfer of hydroxymethyl or formyl groups. EC 2.1.2.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Nitrogenase: An enzyme system that catalyzes the fixing of nitrogen in soil bacteria and blue-green algae (CYANOBACTERIA). EC 1.18.6.1.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Methylococcaceae: A family of gram-negative, aerobic bacteria utilizing only one-carbon organic compounds and isolated from in soil and water.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Gluconacetobacter: A genus in the family ACETOBACTERACEAE comprised of acetate-oxidizing bacteria.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)

Initial reactions in anaerobic oxidation of m-xylene by the denitrifying bacterium Azoarcus sp. strain T. (1/69)

The initial enzymatic steps in anaerobic m-xylene oxidation were studied in Azoarcus sp. strain T, a denitrifying bacterium capable of mineralizing m-xylene via 3-methylbenzoate. Permeabilized cells of m-xylene-grown Azoarcus sp. strain T catalyzed the addition of m-xylene to fumarate to form (3-methylbenzyl)succinate. In the presence of succinyl coenzyme A (CoA) and nitrate, (3-methylbenzyl)succinate was oxidized to E-(3-methylphenyl)itaconate (or a closely related isomer) and 3-methylbenzoate. Kinetic studies conducted with permeabilized cells and whole-cell suspensions of m-xylene-grown Azoarcus sp. strain T demonstrated that the specific rate of in vitro (3-methylbenzyl)succinate formation accounts for at least 15% of the specific rate of in vivo m-xylene consumption. Based on these findings, we propose that Azoarcus sp. strain T anaerobically oxidizes m-xylene to 3-methylbenzoate (or its CoA thioester) via (3-methylbenzyl)succinate and E-(3-methylphenyl)itaconate (or its CoA thioester) in a series of reactions that are analogous to those recently proposed for anaerobic toluene oxidation to benzoyl-CoA. A deuterium kinetic isotope effect was observed in the (3-methylbenzyl)succinate synthase reaction (and the benzylsuccinate synthase reaction), suggesting that a rate-determining step in this novel fumarate addition reaction involves breaking a C-H bond.  (+info)

Theoretical investigation of the [1,2]-sigmatropic hydrogen migration in the mechanism of oxidation of 2-aminobenzoyl-CoA by 2-aminobenzoyl-CoA monooxygenase/reductase. (2/69)

The flavin hydroperoxide at the active site of the mixed-function oxidase 2-aminobenzoyl-CoA monooxygenase/reductase (Azoarcus evansii) transfers an oxygen to the 5-position of the 2-aminobenzoyl-CoA substrate to provide the alkoxide intermediate II(-). Hydrogen migration from C5 to C6 follows this monooxygenation. The nature of the monooxygenation intermediate and plausible competing reactions leading to hydrogen migration have been considered. Ab initio molecular orbital theory has been used to calculate structures and electron distributions in intermediate and transition state structures. Electrostatic potential surface calculations establish that the transition state and product, associated with the C5 to C6 hydrogen transfer, are stabilized by electron distribution to the benzoyl-CoA thioester carbonyl oxygen. This is not so for the transition state and product associated with hydrogen transfer from C5 to C4. The activation energy for the 5, 6-shift is 2.5 kcal/mol lower than that for the 5,4-shift. In addition, the product of the hydrogen 5,6-shift is more stable than is the product of the hydrogen 5,4-shift, by approximately 6 kcal/mol. These results explain why only the shift of hydrogen from C5 to C6 is observed experimentally. Oxygen transfer and hydrogen migration almost coincide in the gas phase (activation energy of approximately 0.6 kcal/mol, equivalent to a single bond vibration). Enzymatic formation of alkoxide II(-) requires its stabilization; thus, the rate constant for its breakdown would be slower than in the gas phase.  (+info)

Biochemical and molecular characterization of phenylacetate-coenzyme A ligase, an enzyme catalyzing the first step in aerobic metabolism of phenylacetic acid in Azoarcus evansii. (3/69)

Phenylacetate-coenzyme A ligase (PA-CoA ligase; AMP forming, EC 6.2. 1.30), the enzyme catalyzing the first step in the aerobic degradation of phenylacetate (PA) in Azoarcus evansii, has been purified and characterized. The gene (paaK) coding for this enzyme was cloned and sequenced. The enzyme catalyzes the reaction of PA with CoA and MgATP to yield phenylacetyl-CoA (PACoA) plus AMP plus PPi. The enzyme was specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. Growth with 4-hydroxyphenylacetate, benzoate, adipate, or acetate did not induce the synthesis of this enzyme. This enzymatic activity was detected very early in the exponential phase of growth, and a maximal specific activity of 76 nmol min(-1) mg of cell protein(-1) was measured. After 117-fold purification to homogeneity, a specific activity of 48 micromol min(-1) mg of protein(-1) was achieved with a turnover number (catalytic constant) of 40 s(-1). The protein is a monomer of 52 kDa and shows high specificity towards PA; other aromatic or aliphatic acids were not used as substrates. The apparent K(m) values for PA, ATP, and CoA were 14, 60, and 45 microM, respectively. The PA-CoA ligase has an optimum pH of 8 to 8.5 and a pI of 6.3. The enzyme is labile and requires the presence of glycerol for stabilization. The N-terminal amino acid sequence of the purified protein showed no homology with other reported PA-CoA ligases. The gene encoding this enzyme is 1, 320 bp long and codes for a protein of 48.75 kDa (440 amino acids) which shows high similarity with other reported PA-CoA ligases. An amino acid consensus for an AMP binding motif (VX2SSGTTGXP) was identified. The biochemical and molecular characteristics of this enzyme are quite different from those of the isoenzyme catalyzing the same reaction under anaerobic conditions in the same bacterium.  (+info)

Characterization of the Azoarcus ribozyme: tight binding to guanosine and substrate by an unusually small group I ribozyme. (4/69)

We report novel chemical properties of the ribozyme derived from the smallest group I intron (subgroup IC3) that comes from the pre-tRNA(Ile) of the bacterium Azoarcus sp. BH72. Despite the small size of the Azoarcus ribozyme (195 nucleotides (nt)), it binds tightly to the guanosine nucleophile (Kd = 15 +/- 3 microM) and exhibits activity at high temperatures (approximately 60-70 degrees C). These features may be due to the two GA3 tetraloop interactions postulated in the intron and the high GC content of the secondary structure. The second order rate constant for the Azoarcus ribozyme, ((k(cat)/Km)S = 8.4 +/- 2.1 x 10(-5) M(-1) min(-1)) is close to that found for the related ribozyme derived from the pre-tRNA(Ile) of the cyanobacterium Anabaena PCC7120. pH dependence studies and kinetic analyses of deoxy-substituted substrates suggest that the chemical cleavage step is the rate-determining process in the Azoarcus ribozyme. This may be due to the short 3-nt guide sequence-substrate pairing present in the Azoarcus ribozyme. Finally, the Azoarcus ribozyme shares features conserved in other group I ribozymes including the pH profile, the stereospecificity for the Rp-phosphorothioate at the cleavage site and the 1000-fold decrease in cleavage rate with a deoxyribonucleoside leaving group.  (+info)

Isolation and characterization of a new denitrifying spirillum capable of anaerobic degradation of phenol. (5/69)

Two kinds of phenol-degrading denitrifying bacteria, Azoarcus sp. strain CC-11 and spiral bacterial strain CC-26, were isolated from the same enrichment culture after 1 and 3 years of incubation, respectively. Both strains required ferrous ions for growth, but strain CC-26 grew better than strain CC-11 grew under iron-limited conditions, which may have resulted in the observed change in the phenol-degrading bacteria during the enrichment process. Strain CC-26 grew on phenol, benzoate, and other aromatic compounds under denitrifying conditions. Phylogenetic analysis of 16S ribosomal DNA sequences revealed that this strain is most closely related to a Magnetospirillum sp., a member of the alpha subclass of the class Proteobacteria, and is the first strain of a denitrifying aromatic compound-degrading bacterium belonging to this group. Unlike previously described Magnetospirillum strains, however, this strain did not exhibit magnetotaxis. It grew on phenol only under denitrifying conditions. Other substrates, such as acetate, supported aerobic growth, and the strain exhibited microaerophilic features.  (+info)

Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. (6/69)

The taxonomic structure of members of the genus Azoarcus sensu lato was reassessed in a polyphasic approach. Two species, Azoarcus communis and Azoarcus indigens, three unnamed species containing diazotrophs associated with Kallar grass roots (groups C, D) and a group of strains (E) isolated from fungi were analysed. They were compared by PAGE analyses of cellular proteins, genomic fingerprints, morphological and nutritional features to new isolates from rice roots. All strains within groups C, D and E containing 5-12 isolates showed group-specific cell and colony morphology and carbon source utilization patterns, with exception of the obligately microaerobic strain BS20-3, a member of group C. All strains, with this exception, also had almost indistinguishable electrophoretic protein patterns and genomic fingerprints generated with tDNA-directed primers, suggesting they belong to the same species. Phylogenetic analyses of almost complete 16S rDNA sequences carried out with three different algorithms (neighbour-joining, maximum-likelihood, parsimony) revealed that Azoarcus sensu lato is not monophyletic. Groups C, D and E formed three distinct lineages located between the Azoarcus/Thauera and the Rhodocyclus clusters. Phylogenetic distances between groups C, D and E were as large as between other genera (93-94% sequence similarity). This suggested they have the rank of three different genera. Since it was possible to differentiate them from each other and other related bacteria by phenotypic features, three new genera with one type species each are proposed: Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.  (+info)

Structure-function relationships of two closely related group IC3 intron ribozymes from Azoarcus and Synechococcus pre-tRNA. (7/69)

The two group IC3 pre-tRNA introns from Azoarcus and Synechococcus share very analogous secondary structures. They are small group I ribozymes that possess only two peripheral domains, P2 and P9. However, the 3'-splice site hydrolysis activity of the Synechococcus ribozyme critically depends on P2 whereas that of Azoarcus does not, indicating that the structure-function relationships of the two ribozymes are strikingly different despite their structural resemblance. To identify the element(s) that determines the catalytic properties of these ribozymes, we undertook analyses of chimeric ribozymes prepared by swapping their structural elements. We found that the difference can be attributed to a small number of nucleotides within the conserved core region. Further analysis by employing in vitro selection revealed that a base triple interaction (P4bp3 x J6/7-2) is a critical element for determining activity and suggests the existence of a novel base quintuple involving the base triple P4bp5 x J8/7-5.  (+info)

Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii. (8/69)

The aerobic metabolism of benzoate in the proteobacterium Azoarcus evansii was reinvestigated. The known pathways leading to catechol or protocatechuate do not operate in this bacterium. The presumed degradation via 3-hydroxybenzoyl-coenzyme A (CoA) and gentisate could not be confirmed. The first committed step is the activation of benzoate to benzoyl-CoA by a specifically induced benzoate-CoA ligase (AMP forming). This enzyme was purified and shown to differ from an isoenzyme catalyzing the same reaction under anaerobic conditions. The second step postulated involves the hydroxylation of benzoyl-CoA to a so far unknown product by a novel benzoyl-CoA oxygenase, presumably a multicomponent enzyme system. An iron-sulfur flavoprotein, which may be a component of this system, was purified and characterized. The homodimeric enzyme had a native molecular mass of 98 kDa as determined by gel filtration and contained 0.72 mol flavin adenine dinucleotide (FAD), 10.4 to 18.4 mol of Fe, and 13.3 to 17.9 mol of acid-labile sulfur per mol of native protein, depending on the method of protein determination. This benzoate-induced enzyme catalyzed a benzoyl-CoA-, FAD-, and O2-dependent NADPH oxidation surprisingly without hydroxylation of the aromatic ring; however, H2O2 was formed. The gene (boxA, for benzoate oxidation) coding for this protein was cloned and sequenced. It coded for a protein of 46 kDa with two amino acid consensus sequences for two [4Fe-4S] centers at the N terminus. The deduced amino acid sequence showed homology with subunits of ferredoxin-NADP reductase, nitric oxide synthase, NADPH-cytochrome P450 reductase, and phenol hydroxylase. Upstream of the boxA gene, another gene, boxB, encoding a protein of 55 kDa was found. The boxB gene exhibited homology to open reading frames in various other bacteria which code for components of a putative aerobic phenylacetyl-CoA oxidizing system. The boxB gene product was one of at least five proteins induced when A. evansii was grown on benzoate.  (+info)

*Azoarcus

... indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus is a genus of nitrogen-fixating bacteria. Species in this genus are usually found in contaminated water, as they are ... ISBN 0-7923-4514-2. LPSN Azoarcus at the Encyclopedia of Life. ... Azoarcus spp., by analyses of partial 16S ribosomal DNA ... "Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and ...

*Azoarcus olearius

... is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are Gram-negative, motile and rod- ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... ISBN 0-7923-4514-2. LPSN Type strain of Azoarcus olearius at BacDive - the Bacterial Diversity Metadatabase. ... "Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil". International Journal of ...

*Azoarcus anaerobius

LSPN bacterio.net Straininfo of Azoarcus anaerobius Springer, N; Ludwig, W; Philipp, B; Schink, B (1998). "Azoarcus anaerobius ... Azoarcus anaerobius is a gram-negative, strictly anaerobic, catalase-negative, nitrate-reducing, rod-shaped bacterium from the ... UniProt International Journal of Systematic Bacteriology [1] Type strain of Azoarcus anaerobius at BacDive - the Bacterial ...

*Azoarcus communis

ISBN 0-7923-4514-2. Azoarcus communis at the Encyclopedia of Life LPSN Type strain of Azoarcus communis at BacDive - the ... Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus communis is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are yellow-pigmented, straight to ... Reinhold-Hurek, B.; Hurek, T.; Gillis, M.; Hoste, B.; Vancanneyt, M.; Kersters, K.; De Ley, J. (1993). "Azoarcus gen. nov., ...

*Azoarcus indigens

... at the Encyclopedia of Life LPSN Type strain of Azoarcus indigens at BacDive - the Bacterial Diversity ... Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus indigens is a species of bacteria. It is a nitrogen-fixing bacteria associated with roots of Leptochloa fusca . Its ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ...

*Azoarcus toluvorans

... is a bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus toluvorans Song, B; ... and Azoarcus toluclasticus sp. nov". Int J Syst Bacteriol. 49 Pt 3: 1129-40. doi:10.1099/00207713-49-3-1129. PMID 10425771. ... Type strain of Azoarcus toluvorans at BacDive - the Bacterial Diversity Metadatabase. ... "Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. ...

*Azoarcus toluclasticus

... is a gram negative bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus ... and Azoarcus toluclasticus sp. nov". Int J Syst Bacteriol. 49 Pt 3: 1129-40. doi:10.1099/00207713-49-3-1129. PMID 10425771. ... "Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. ...

*Azoarcus buckelii

... is a bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus buckelii The Taxonomicon ... nov., and Azoarcus buckelii sp. nov". Arch Microbiol. 178: 26-35. doi:10.1007/s00203-002-0422-6. PMID 12070766. Type strain of ... Azoarcus buckelii at BacDive - the Bacterial Diversity Metadatabase. ...

*Azoarcus evansii

... is a species of bacteria. Its type strain is KB 740T. This particular strain of Azoarcus evansii has been ... Azoarcus evansii at the Encyclopedia of Life Type strain of Azoarcus evansii at BacDive - the Bacterial Diversity Metadatabase ... nov., and Azoarcus, as Azoarcus evansii sp. nov., Respectively, Members of the Beta Subclass of the Proteobacteria". ... The Azoarcus evansii consumes (literally eats) the Lactobacillus johnsonii to maintain a healthy population level. It has been ...

*Azoarcus tolulyticus

... is a species of bacteria. It is a nitrogen-fixing bacteria. It is notable for degrading toluene. Tol-4 is ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... LPSN Azoarcus tolulyticus at the Encyclopedia of Life. ... Azoarcus tolulyticus, with small-subunit rRNA primers and ... "Phylogenetic Analyses of a New Group of Denitrifiers Capable of Anaerobic Growth on Toluene and Description of Azoarcus ...

*Thauera phenylacetica

nov., and Azoarcus buckelii sp. nov". Arch Microbiol. 178: 26-35. doi:10.1007/s00203-002-0422-6. PMID 12070766. UniProt Type ...

*Thauera aromatica

nov., and Azoarcus, as Azoarcus evansii sp. nov., Respectively, Members of the Beta Subclass of the Proteobacteria". ...

*S)-1-phenylethanol dehydrogenase

Kniemeyer, O.; Heider, J. (2001). "(S)-1-phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ...

*Aromatoleum

... and is officially it is referred to Azoarcus sp. EbN1 as it belongs to the Azoarcus/Thauera cluster. The discovery of the ...

*Benzoyl-CoA 2,3-dioxygenase

3-dioxygenase is involved in aerobic benzoate metabolism in Azoarcus evansii. Zaar, A.; Gescher, J.; Eisenreich, W.; Bacher, A ... "Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii". J. Bacteriol. 184 (22): 6301-6315. doi: ... "Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii". J. Bacteriol. 183 (6): ... Fuchs, G. (2004). "New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii". Mol. Microbiol. 54 (1): 223-238. ...

*Rhodocyclales

Uliginosibacterium and Azoarcus. Cells are rod shaped. Dominant respiratory quinones are ubiquinone-8 and rhodoquinone-8. G+C ...

*Cyclohexane-1,2-dione hydrolase

Harder, J. (1997). "Anaerobic degradation of cyclohexane-1,2-diol by a new Azoarcus species". Arch. Microbiol. 168: 199-204. ...

*Benzoyl-CoA-dihydrodiol lyase

Gescher, J.; Eisenreich, W.; Worth, J.; Bacher, A.; Fuchs, G. (2005). "Aerobic benzoyl-CoA catabolic pathway in Azoarcus ... formate The enzyme is involved in the aerobic benzoyl-CoA catabolic pathway in Azoarcus evansii. ...

*Phenylglyoxylate dehydrogenase (acylating)

... a new enzyme of anaerobic phenylalanine metabolism in the denitrifying bacterium Azoarcus evansii". Eur. J. Biochem. 251 (3): ...

*Ethylbenzene hydroxylase

... a denitrifying bacterium related to the genera Azoarcus and Thauera. It is a molybdenum enzyme belonging to the DMSO reductase ...

*Hydroxy-1,4-benzoquinone

... by the denitrifying bacterium Azoarcus anaerobius". Journal of Bacteriology. 180 (14): 3644-9. PMC 107334 . PMID 9658009. ...

*3,4-Dehydroadipyl-CoA semialdehyde dehydrogenase (NADP+)

... catabolic pathway in Azoarcus evansii: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase ... H+ This enzyme catalyses a step in the aerobic benzoyl-coenzyme A catabolic pathway in Azoarcus evansii and Burkholderia ...

*Hypercycle (chemistry)

used an Azoarcus group I intron ribozyme that, when fragmented, has an ability to self-assemble by catalysing recombination ... akin to a recombination of Azoarcus ribozyme from four inactive oligonucleotide fragments described earlier. Apart from a ...

*Azospira oryzae

Reinhold-Hurek, B.; Hurek, T. (2000). "Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato ...

*Azonexus fungiphilus

Reinhold-Hurek, B.; Hurek, T. (2000). "Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato ...
Krause, A., H. Julich, M. Mankar. and B.Reinhold-Hurek, (2017) The Regulatory Network Controlling Ethanol-Induced Expression of Alcohol Dehydrogenase in the Endophyte Azoarcus sp. Strain BH72. Mol Plant-Microbe Interact 30: DOI:doi.org/10.1094/MPMI-01-17-0013-R. Sarkar, A., M. Marszalkowska, M. Schäfer, T. Pees, H. Klingenberg, F. Macht & B. Reinhold-Hurek, (2016) Global expression analysis of the response to microaerobiosis reveals an important cue for endophytic establishment of Azoarcus sp. BH72. Environ Microbiol 19: 198-217.. Grönemeyer, J.L., T. Hurek & B. Reinhold-Hurek, (2015) Bradyrhizobium kavangense sp. nov., a symbiotic nitrogen-fixing bacterium from root nodules of traditional Namibian pulses. Int. J. Syst. Evol. Microbiol. 65: 4886-4894.. Reinhold-Hurek, B., W. Bünger, C.S. Burbano, M. Sabale & T. Hurek, (2015) Roots shaping their microbiome: Global hot spots for microbial activity. Annu. Rev. Phytopathol. 53: 403-424.. Burbano, C.S., J.L. Grönemeyer, T. Hurek & B. ...
Ray, S. K., Rajeshwari, R., Shar ma, Y., and Sonti, R. V. 2002. A high-molecular-weight outer membrane protein of Xanthomonas oryzae pv. oryzae exhibits similarity to nonfimbrial adhesins of animal pathogeni c bacteria and is required for optimum virulence. Mol. Microbiol. 46:637-647. Regu, M., Enfedaque, J., Camprubi, S., and Toms, J. M. 1992. The O-antigen lipopolysaccharide is the major barrier to plasmid DNA uptake by Klebsiella pneumoniae during transformation by electroporation and os motic shock. J. Microbiol. Methods 15:129-134. Reinhold-Hurek, B., Maes, T., Gemmer, S., Van Montagu, M., and Hurek, T. 2006. An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp strain BH72. Mol. Plan t-Microbe Interact. 19:181-188. Reiter, B., Brgmann, H., Burg, K., and Sessitsch, A. 2003. Endophytic nifH gene diversity in African sweet potato. Can. J. Microbiol. 49:549-555. Reslewic, S., Zhou, S., Place, M., Zhang, Y. P., Briska, A., Goldstein, S., ...
Azoarcus sp. strain BH72 is a mutualistic N2-fixing endophyte of salt-tolerant Kallar grass (Leptochloa fusca (L.) Kunth), a grass gowing in the Punjab of Pakistan. Endophytes are bacteria that live within the tissues of plants without causing them any harm; Azoarcus resides within the roots of its host. Azoarcus sp. strain BH72 is also capable of endophytic N2 fixation in rice and sugarcane. The bacteria has not been detected in root-free soil, although it is able to be cultured. It does not however excrete significant amounts of nitrogenous compounds in culture. The genome sequence has shown that Azoarcus has very few mobile genetic elements, or phage-related genes, suggesting its adaptation to a fairly stable, low-stress environment. The sec-dependent, SRP-mediated and Tat systems for protein secretion are all present, in addition to a type I, the type IIb secretion systems and an autotransporter. However it is lacking both type III and type IV secretion systems, suggesting it can not export ...
The enzyme is involved in the aerobic benzoyl-CoA catabolic pathway of the bacterium Azoarcus evansii. The enzyme converts 2,3-epoxy-2,3-dihydrobenzoyl-CoA to its oxepin form prior to the ring-opening and the formation of a dialdehyde intermediate ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX complex in yeast), processing of ends, and recruitment of the DNA ligase IV (LIG4)-XRCC4 complex, which brings about ligation. A recent study shows that bacteria accomplish NHEJ using just two proteins (Ku and DNA ligase), whereas eukaryotes require many factors. NHEJ repairs DSBs at all stages of the cell cycle, bringing about the ligation of two DNA DSBs without the need for sequence homology, and so is error-prone ...
SWISS-MODEL Repository entry for A1KAA5 (PANB_AZOSB), 3-methyl-2-oxobutanoate hydroxymethyltransferase. Azoarcus sp (strain BH72)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
The first stage, glycolysis is the anaerobic catabolism of glucose, it occurs in almost all cells. The process uses glucose and co-enzyme NAD (Nicotinamide Adenine Dinucleotide), and yields 2 molecules of Pyruvic acid, as below C6H12O6 + 2NAD+ -, 2C3H4O3 + 2NADH + 2H+ The free energy stored in 2 molecules of pyruvic acid is somewhat less than that in the original glucose molecule. Some of this difference is captured in 2 molecules of ATP. The Krebs Cycle then decarboxylates the pyruvic acid resulting in a 2-carbon fragment of acetate. This 2-carbon fragment is coupled to a molecule of oxaloacetic acid , this results in a molecule of citric acid (the process is also known as the citric acid cycle) ...
SOLUTION STRUCTURE OF A 22-NUCLEOTIDE HAIRPIN SIMILAR TO THE P5ABC REGION OF A GROUP I RIBOZYME WITH COBALT(III)HEXAMMINE COMPLEXED TO THE GAAA TETRALOOP. ...
RNA. 2011 Nov;17(11):1967-72. doi: 10.1261/rna.2757311. Epub 2011 Sep 29. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
These pumps disappeared in excised root segments. In excised root tips, there appeared to be weak K+ pumps at both sites in the ...
PK 11195 | BZD antagonist | PK11195 | CAS [85532-75-8] | Axon 1208 | Axon Ligand™ with >99% purity available from supplier Axon Medchem, prime source of life science reagents for your research
Plasmid CIB81-pmGFP from Dr. Chandra Tuckers lab contains the insert CIB1 and is published in Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. This plasmid is available through Addgene.
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href="http://www.uniprot.org/manual/proteomes_manual">proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
prephenic acid: nonaromatic biosynthetic intermediate, probable immediate precursor of aromatic systems such as phenylalanine, tyrosine, tannins, flavanoids; isolated from cultures of mutant E coli; structure
We next selected several positions based on their close proximity and direct atomic interactions with EVT-101. Disruption of the key π-stacking interaction with the central pyridazine ring of EVT-101 by mutating GluN1-Y109 into the nonaromatic and short serine resulted in a drastic decrease in EVT-101 sensitivity (800-fold increase in IC50). In contrast, sensitivity to ifenprodil was barely affected (,2-fold change in IC50; Fig. 3B, and see also the GluN1-Y109C mutation in Table 1), thus providing strong evidence for the critical importance of residue GluN1-Y109 in mediating EVT-101 inhibitory action. We obtained additional support for the relevance of the EVT-101 binding site by investigating mutations at positions GluN2B-Q110 (α2 helix) and GluN2B-A135 (β4-β5 loop). As described above, both residues line the EVT-101 cavity, making specific contacts with the ligand. Substituting GluN2B-Q110 by a glycine to avoid any side chain-ligand interaction strongly decreased EVT-101 sensitivity ...
Disclosed are polymeric dyes of formula (1a), (1b), or (1c); wherein A and B, independently from each other represent a polymer backbone; X1 and X2 independently from each other are a linkage group selected from --S--, --S--S--, --N--, --N═--, --N(R5)--, --S(O)--, --SO2--, --(CH2CH2--O)1-5--, --(CH2CH2CH2--O)1-5--, --C(O)--, --C(O)O--, --OCO--, (formula II) --CON(R1)--, --C(NR1R2)2--, --(R1)NC(O)--, --C(S)R1--; which may be interrupted and/or terminated at one or both ends by one or more than one --C1C30alkylene- or --C2-C12alkenylene-; or an optionally substituted, saturated or unsaturated, fused or non-fused aromatic or nonaromatic (heterocyclic) bivalent radical optionally comprising at least one heteroatom; a saturated or unsaturated, fused or non-fused aromatic or nonaromatic bivalent radical comprising at least one heteroatom, which is optionally substituted by C1-C30alkyl, C1-C30alkoxy, C2-C12alkenyl, C5-C10aryl, C5-C10cycloalkyl, C1-C10alkyl(C5-C10arylene), hydroxy or halogen; R1 and ...
The April 2014 edition of the New Journal of Botany (Volume 4 Number 1) contains the following article: British Sorbus (Rosaceae): six new species, two hybrids and a new subgenus by T. C. G. Rich, D. Green, L. Houston, M. Lepší, S. Ludwig and J. Pellicer.. The new species are Evanss Whitebeam (Sorbus evansii), Greens Whitebeam (S. greenii), Herefordshire Whitebeam (S. herefordensis), Richs Whitebeam (S. richii), Sells Whitebeam (S. sellii) and Observatory Whitebeam (S. spectans). These all grow around the Severn and Severn estuary and the article gives maps and details of locations with detailed descriptions of the species should anyone want to go and see them.. The new hybrids are Sorbus aucuparia x S. minima where a single example has been found in the Brecon Beacons and Sorbus porrigentiformis x. S. torminalis with two trees on a cliff in Monmouthshire.. All these species and hybrids have been defined as distinct using genetic analysis techniques.. The new subgenus is Triparens, set up ...
The wide, but scattered distribution of group I introns in nature is a result of two processes; the vertical inheritance of introns with or without losses, and the occasional transfer of introns across species barriers. Reversal of the group I intron
Novel Group 4, Group 3 or Lanthanide metal complexes wherein the metal is in the +3 formal oxidation state containing a cyclic or non-cyclic, nonaromatic, anionic, dienyl ligand group bound to M and having a bridged ligand structure, catalytic derivatives of such complexes; and the use thereof as catalysts for polymerizing addition polymerizable monomers are disclosed.
Kink definition is - a short tight twist or curl caused by a doubling or winding of something upon itself. How to use kink in a sentence.
A novel organocatalyst was developed that effectively catalyzed the reactions of unprotected or protected dihydroxyacetone with a variety of aldehydes to provide syn-aldol products with good yields and ee values up to >99%. Significantly, this amide catalyst was effective with a variety of nonaromatic aldehyde acceptors that had proven difficult in the presence of other catalysts. Reactions of protected dihydroxyacetone proceeded in aqueous media without addition of organic solvents ...
Glucosepane is a structurally complex protein posttranslational modification that is believed to exist in all living organisms. Research in humans suggests that glucosepane plays a critical role in the pathophysiology of both diabetes and human aging, yet comprehensive biological investigations of this metabolite have been hindered by a scarcity of chemically homogeneous material available for study. Here we report the total synthesis of glucosepane, enabled by the development of a one-pot method for preparation of the nonaromatic 4H-imidazole tautomer in the core. Our synthesis is concise (eight steps starting from commercial materials), convergent, high-yielding (12% overall), and enantioselective. We expect that these results will prove useful in the art and practice of heterocyclic chemistry and beneficial for the study of glucosepane and its role in human health and disease. ...
acenol, acerol, acetarsol, acmiol, acyloglicerol, Adampol, adinol, Adrianopol, aerosol, aerozol, akropol, Akropol, akseroftol, aldol, ALGOL, Algol, alizarol, alkohol, alumatol, amatol, amfibol, amidol, aminoalkohol, aminofenol, amol, Anatol, Andrespol, anetol, angol, Angol, Annopol, anticol, antikol, antresol, antymetabol, AOL, apiol, apsydiol, archeol, atol, babol, banderol, barkarol, bazgrol, bejsbol, bemol, benzohol, benzol, benzopirol, biadol, Bibiampol, bioetanol, biometanol, bisabolol, bombykol, borazol, borneol, bristol, Bristol, brizol, Brol, bromofenol, brystol, bryzol, bucol, busol, butanol, butapirazol, bzdęgol, bzdręgol, Capitol, carmagnol, carol, centriol, certol, Chabrol, chinoksyzol, chinol, Chinol, chinozol, chloramfenikol, cholekalcyferol, cholesterol, chromol, chryzokol, cibazol, COBOL, Cobol, Colargol, control, Control, cool, cykloheksanol, cyneol, cytronelol, czebol, dehydrocholesterol, dehydroretinol, dekapol, Dekapol, dermatol, diabetol, dikumarol, dimerkaprol, dipol, ...
acenol, acerol, acetarsol, acmiol, acyloglicerol, Adampol, adinol, Adrianopol, aerosol, aerozol, akropol, Akropol, akseroftol, aldol, ALGOL, Algol, alizarol, alkohol, alumatol, amatol, amfibol, amidol, aminoalkohol, aminofenol, amol, Anatol, Andrespol, anetol, angol, Angol, Annopol, anticol, antikol, antresol, antymetabol, AOL, apiol, apsydiol, archeol, atol, babol, banderol, barkarol, bazgrol, bejsbol, bemol, benzohol, benzol, benzopirol, biadol, Bibiampol, bioetanol, biometanol, bisabolol, bombykol, borazol, borneol, bristol, Bristol, brizol, Brol, bromofenol, brystol, bryzol, bucol, busol, butanol, butapirazol, bzdęgol, bzdręgol, Capitol, carmagnol, carol, centriol, certol, Chabrol, chinoksyzol, chinol, Chinol, chinozol, chloramfenikol, cholekalcyferol, cholesterol, chromol, chryzokol, cibazol, COBOL, Cobol, Colargol, control, Control, cool, cykloheksanol, cyneol, cytronelol, czebol, dehydrocholesterol, dehydroretinol, dekapol, Dekapol, dermatol, diabetol, dikumarol, dimerkaprol, dipol, ...
Cyanothece sp. ATCC ® 51142D-5™ Designation: Genomic DNA from Cyanothece sp. strain BH68 TypeStrain=False Application:
Cyanothece sp. ATCC ® 51142D-5™ Designation: Genomic DNA from Cyanothece sp. strain BH68 TypeStrain=False Application:
View Notes - Lecture8 from BIOC 100A at UCSC. 25 26 The hammerhead ribozyme (plant virus) Martick & Scott, Cell 2006 27 Group I intron ribozyme Golden et al, and cech Science (1998) 28 Acid-Base

Azoarcus - WikipediaAzoarcus - Wikipedia

Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus is a genus of nitrogen-fixating bacteria. Species in this genus are usually found in contaminated water, as they are ... ISBN 0-7923-4514-2. LPSN Azoarcus at the Encyclopedia of Life. ... Azoarcus spp., by analyses of partial 16S ribosomal DNA ... "Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and ...
more infohttps://en.wikipedia.org/wiki/Azoarcus

Azoarcus olearius - WikipediaAzoarcus olearius - Wikipedia

Azoarcus olearius is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are Gram-negative, motile and rod- ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... ISBN 0-7923-4514-2. LPSN Type strain of Azoarcus olearius at BacDive - the Bacterial Diversity Metadatabase. ... "Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil". International Journal of ...
more infohttps://en.wikipedia.org/wiki/Azoarcus_olearius

KEGG PATHWAY: Non-homologous end-joining - Azoarcus sp. BH72KEGG PATHWAY: Non-homologous end-joining - Azoarcus sp. BH72

Non-homologous end-joining - Azoarcus sp. BH72 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?azo03450+azo0444

tcp - Putative citrate chemoreceptor protein - Azoarcus sp. (strain BH72) - tcp gene & proteintcp - Putative citrate chemoreceptor protein - Azoarcus sp. (strain BH72) - tcp gene & protein

Azoarcus sp. (strain BH72)Imported. ,p>Information which has been imported from another database using automatic procedures.,/p ... tr,A1K5G6,A1K5G6_AZOSB Putative citrate chemoreceptor protein OS=Azoarcus sp. (strain BH72) GN=tcp PE=4 SV=1 ...
more infohttp://www.uniprot.org/uniprot/A1K5G6

DIGITAL.CSIC: Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.DIGITAL.CSIC: Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.

Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.. Authors: Durante-Rodríguez, Gonzalo ... promoter responsible for the anaerobic catabolism of benzoate in Azoarcus sp. CIB constitutes the prototype of a new subfamily ...
more infohttp://digital.csic.es/handle/10261/52529

Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T. | Alfred M. Spormann LaboratorySubstrate range of benzylsuccinate synthase from Azoarcus sp. strain T. | Alfred M. Spormann Laboratory

Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T.. Title. Substrate range of benzylsuccinate synthase ... In substrate range studies with partially purified benzylsuccinate synthase from denitrifying Azoarcus sp. strain T, ...
more infohttp://web.stanford.edu/group/spormannlab/cgi-bin/amslab/content/substrate-range-benzylsuccinate-synthase-azoarcus-sp-strain-t

Exploring the Function of Alcohol Dehydrogenases During the Endophytic Life of Azoarcus Sp. Strain BH72Exploring the Function of Alcohol Dehydrogenases During the Endophytic Life of Azoarcus Sp. Strain BH72

The endophytic bacterium Azoarcus sp. strain BH72 is capable of colonizing the interior of rice roots, where it finds suitable ... The endophytic bacterium Azoarcus sp. strain BH72 is capable of colonizing the interior of rice roots, where it finds suitable ... Thus, it is likely that ethanol is an important carbon source for the endophytic life of Azoarcus sp. ... fusion library of Azoarcus sp. grown under different conditions was performed. Monitoring of the GFP activity allowed the ...
more infohttps://www.apsnet.org/publications/mpmi/2011/November/Pages/24_11_1325.aspx

Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp....Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp....

The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus ( ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ... and Azoarcus toluclasticus sp. nov.. Song B1, Häggblom MM, Zhou J, Tiedje JM, Palleroni NJ. ... A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10425771?dopt=Abstract

The oil-contaminated soil diazotroph Azoarcus olearius DQS-4T is genetically and phenotypically similar to the model grass...The oil-contaminated soil diazotroph Azoarcus olearius DQS-4T is genetically and phenotypically similar to the model grass...

Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63T and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that DQS-4 ... was sequenced and compared with other Azoarcus strains. The genome sequence showed high synteny with Azoarcus sp. BH72, a model ... The genome of Azoarcus olearius DQS-4T , a N2 -fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, ... The oil-contaminated soil diazotroph Azoarcus olearius DQS-4T is genetically and phenotypically similar to the model grass ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/27893193

Benzylfumaric, benzylmaleic, and Z- and E-phenylitaconic acids: synthesis, characterization, and correlation with a metabolite...Benzylfumaric, benzylmaleic, and Z- and E-phenylitaconic acids: synthesis, characterization, and correlation with a metabolite...

E-Phenylitaconic acid has been isolated as a metabolite generated by Azoarcus tolulyticus Tol-4 along with benzylsuccinic acid ... and Azoarcus toluclasticus sp. nov.. *Binwei Song, Max M Häggblom, Jian-ping Zhou, James M. Tiedje, Norberto J. Palleroni ... Initial reactions in anaerobic oxidation of m-xylene by the denitrifying bacterium Azoarcus sp. strain T.. *Cynthia J. Krieger ... E-Phenylitaconic acid has been isolated as a metabolite generated by Azoarcus tolulyticus Tol-4 along with benzylsuccinic acid ...
more infohttps://www.semanticscholar.org/paper/Benzylfumaric%2C-benzylmaleic%2C-and-Z-and-acids%3A-and-a-Migaud-Chee-Sanford/d74e432a7c595ce8b7180b9ef3bb9481b690a7e6

Microbiology Society Journals | Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and...Microbiology Society Journals | Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and...

The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus ( ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ... A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ...
more infohttp://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-3-1129

Physical map of the Azoarcus sp strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome...Physical map of the Azoarcus sp strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome...

Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of ... We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as ... Battistoni F, Bartels D, Kaiser O, Reamon-Buettner SM, Hurek T, Reinhold-Hurek B. Physical map of the Azoarcus sp strain BH72 ... Physical map of the Azoarcus sp strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome ...
more infohttps://pub.uni-bielefeld.de/publication/1602459

Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01|sup|T|/supDraft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01|sup|T|/sup

Azoarcus sp. strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It ... Azoarcus sp. strain PA01,sup,T,/sup, belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class ... Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01,sup,T,/sup,. * Home ... Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01,sup,T,/sup,. 2015. eng. ...
more infohttps://kops.uni-konstanz.de/handle/123456789/32259

Characterization of halobenzoate-degrading, denitrifying Azoarcus and Thauera isolates and description of Thauera...Characterization of halobenzoate-degrading, denitrifying Azoarcus and Thauera isolates and description of Thauera...

DNA-DNA hybridization experiments established that strains 2FB2, 2FB6 and 4FB10 belong to the species Azoarcus tolulyticus. ... 2FB6 and 4FB10 as new members of the genus Azoarcus and strains 4FB1, 4FB2, 3CB2, 3CB3 and 3BB1 as new members of the genus ... The taxonomic relationships of Azoarcus and Thauera isolates in the beta-subclass of the Proteobacteria capable of degrading ... The taxonomic relationships of Azoarcus and Thauera isolates in the beta-subclass of the Proteobacteria capable of degrading ...
more infohttps://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-51-2-589

Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01T | Standards in Genomic...Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01T | Standards in Genomic...

strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It is a ... strain KH32C [23] and Azoarcus sp. strain BH72 [24] and Azoarcus toluclasticus ATCC 700605 [25]. The exact phylogenetic ... Azoarcus sp. strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It ... Azoarcus buckelii DSM 14744T (99 % gene similarity) [14, 15] and Azoarcus anaerobius (98 %) [16]. A. buckelii DSM 14744T was ...
more infohttps://standardsingenomics.biomedcentral.com/articles/10.1186/s40793-015-0079-9

Kegg pathway models for JSimKegg pathway models for JSim

Organism azo: Azoarcus sp. BH72. to run JSim applets. See JSim Kegg models page for comlete list of Kegg models. ...
more infohttp://www.physiome.org/jsim/models/kegg/organism.html?azo

nature.com searchnature.com search

Correction: Corrigendum: Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 *Andrea Krause ... Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 *Andrea Krause ... Rights & permissionsfor article Complete genome of the mutualistic, N,sub,2,/sub,-fixing grass endophyte ,i,Azoarcus,/i, sp. ... Azoarcus,/i, sp. strain BH72 . Opens in a new window. ...
more infohttp://www.nature.com/search?author=%22Alice+C.+McHardy%22&error=cookies_not_supported&code=0aa6335f-05bd-49b0-8f78-2bb766e46ee3

SWISS-MODEL Repository | A1K601SWISS-MODEL Repository | A1K601

A1K601 (LEPA_AZOSB) Azoarcus sp (strain BH72). Elongation factor 4 UniProtKBInterProSTRINGInteractive Modelling. 598 aa; ...
more infohttps://swissmodel.expasy.org/repository/uniprot/A1K601

SWISS-MODEL Repository | A1KAA5SWISS-MODEL Repository | A1KAA5

A1KAA5 (PANB_AZOSB) Azoarcus sp (strain BH72). 3-methyl-2-oxobutanoate hydroxymethyltransferase UniProtKBInterProSTRING ...
more infohttps://swissmodel.expasy.org/repository/uniprot/A1KAA5

Proteins matched: G3DSA:3.90.20.20 (G3DSA:3.90.20.20) | InterPro | EMBL-EBIProteins matched: G3DSA:3.90.20.20 (G3DSA:3.90.20.20) | InterPro | EMBL-EBI

Azoarcus sp. (strain BH72). Loading... A1KFH3 Protein GrpE. Mycobacterium bovis (strain BCG / Pasteur 1173P2). Loading... ...
more infohttp://www.ebi.ac.uk/interpro/ISignatureProteins?sig=G3DSA:3.90.20.20&query=X

Proteins matched: SSF52733 (SSF52733) | InterPro | EMBL-EBIProteins matched: SSF52733 (SSF52733) | InterPro | EMBL-EBI

Azoarcus sp. (strain BH72). Loading... A1KKP9 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase. ...
more infohttp://www.ebi.ac.uk/interpro/ISignatureProteins?sig=SSF52733&query=Y

Plants  | Free Full-Text | Molecular Analyses of the Distribution and Function of Diazotrophic Rhizobia and Methanotrophs in...Plants | Free Full-Text | Molecular Analyses of the Distribution and Function of Diazotrophic Rhizobia and Methanotrophs in...

Azoarcus sp. BH72. Paenibacillus odorifer. Tuber harvested in Oct. 2002, and Oct. 2004 from cv. Beniazuma grown on an Andozol, ... Azoarcus sp. BH72. Clostridium pasteurianum. Stem harvested in Oct. 2002, Aug. 2004 and Oct. 2004 from cv. Beniazuma grown in ... β-Proteobacteria (Azoarcus sp., Azospira oryzae, Azotobacter sp.) γ-Proteobacteria (Methylococcus) δ-Proteobacteria (Geobacter ... and Azoarcus sp. BH72 (Table 6). The nifH DNA was also isolated from the stem and tuber samples from several-months-old sweet ...
more infohttps://www.mdpi.com/2223-7747/8/10/408/htm

Frontiers | Microbial Inoculants for Improving Crop Quality and Human Health in Africa | MicrobiologyFrontiers | Microbial Inoculants for Improving Crop Quality and Human Health in Africa | Microbiology

Symbiotic nitrogen fixers include the following genera, Rhizobium, Achromobacter, Sinorhizobium, Azoarcus, Mesorhizobium, ... Azoarcus sp., Herbaspirillum sp., Gluconacetobacter diazotrophicus, and Azotobacter sp. (Vessey, 2003). ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02213/full
  • Exploring the Function of Alcohol Dehydrogenases During the Endophytic Life of Azoarcus Sp. (apsnet.org)
  • Thus, it is likely that ethanol is an important carbon source for the endophytic life of Azoarcus sp. (apsnet.org)
  • However, a renewed interest in endophytic diazotrophs, such as Acetobacter , Azoarcus, and Herbaspirillum , in gramineous plants has arisen because of their occurrence mainly within plant tissues and evidence for significant nitrogen fixation ( 18 , 25 ). (asm.org)
  • Endophytic diazotrophic bacteria ( Azoarcus spp . (uni-bremen.de)
  • Sarkar, A., M. Marszalkowska, M. Schäfer, T. Pees, H. Klingenberg, F. Macht & B. Reinhold-Hurek, (2016) Global expression analysis of the response to microaerobiosis reveals an important cue for endophytic establishment of Azoarcus sp. (uni-bremen.de)
  • Because these properties are poorly understood, a microtiter-plate-based screening of a transcriptional gfp (green fluorescent protein) fusion library of Azoarcus sp. (apsnet.org)
  • An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. (uni-bielefeld.de)