A genus of gram-negative, facultatively anaerobic bacteria including species which are often associated with grasses (POACEAE) and which fix nitrogen as well as species which anaerobically degrade toluene and other mono-aromatic hydrocarbons.
A large group of facultatively anaerobic, rod-shaped bacteria that show up as pink (negative) when treated by the gram-staining method.
A genus of gram-negative, rod-shaped bacteria able to anaerobically oxidize and degrade toluene.
A widely used industrial solvent.
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Inorganic compounds that contain nitrogen as an integral part of the molecule.
A family of isomeric, colorless aromatic hydrocarbon liquids, that contain the general formula C6H4(CH3)2. They are produced by the destructive distillation of coal or by the catalytic reforming of petroleum naphthenic fractions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.
A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.
Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
An antiseptic and disinfectant aromatic alcohol.
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
An endosymbiont that is either a bacterium or fungus living part of its life in a plant. Endophytes can benefit host plants by preventing pathogenic organisms from colonizing them.
The repair of DOUBLE-STRAND DNA BREAKS by rejoining the broken ends of DNA to each other directly.
Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently.
Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 6.5.1.1 (ATP) and EC 6.5.1.2 (NAD).
Immunologically detectable substances found in the CELL NUCLEUS.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
A serine-threonine protein kinase that, when activated by DNA, phosphorylates several DNA-binding protein substrates including the TUMOR SUPPRESSOR PROTEIN P53 and a variety of TRANSCRIPTION FACTORS.
The poppy plant family of the order Papaverales, subclass Magnoliidae, class Magnoliopsida. These have bisexual, regular, cup-shaped flowers with one superior pistil and many stamens; 2 or 3 conspicuous, separate sepals and a number of separate petals. The fruit is a capsule. Leaves are usually deeply cut or divided into leaflets.
Cells specialized to detect chemical substances and relay that information centrally in the nervous system. Chemoreceptor cells may monitor external stimuli, as in TASTE and OLFACTION, or internal stimuli, such as the concentrations of OXYGEN and CARBON DIOXIDE in the blood.
A plant genus of the family Plantaginaceae. Members contain DEFICIENS PROTEIN.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The protein complement of an organism coded for by its genome.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Computer-based representation of physical systems and phenomena such as chemical processes.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The functions and properties of living organisms, including both the physical and chemical factors and processes, supporting life in single- or multi-cell organisms from their origin through the progression of life.
Sequential operating programs and data which instruct the functioning of a digital computer.
The functions and activities of living organisms that support life in single- or multi-cellular organisms from their origin through the progression of life.
Theoretical representations that simulate the behavior or activity of systems, processes, or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
An enzyme system that catalyzes the fixing of nitrogen in soil bacteria and blue-green algae (CYANOBACTERIA). EC 1.18.6.1.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
A family of gram-negative, aerobic bacteria utilizing only one-carbon organic compounds and isolated from in soil and water.

Initial reactions in anaerobic oxidation of m-xylene by the denitrifying bacterium Azoarcus sp. strain T. (1/69)

The initial enzymatic steps in anaerobic m-xylene oxidation were studied in Azoarcus sp. strain T, a denitrifying bacterium capable of mineralizing m-xylene via 3-methylbenzoate. Permeabilized cells of m-xylene-grown Azoarcus sp. strain T catalyzed the addition of m-xylene to fumarate to form (3-methylbenzyl)succinate. In the presence of succinyl coenzyme A (CoA) and nitrate, (3-methylbenzyl)succinate was oxidized to E-(3-methylphenyl)itaconate (or a closely related isomer) and 3-methylbenzoate. Kinetic studies conducted with permeabilized cells and whole-cell suspensions of m-xylene-grown Azoarcus sp. strain T demonstrated that the specific rate of in vitro (3-methylbenzyl)succinate formation accounts for at least 15% of the specific rate of in vivo m-xylene consumption. Based on these findings, we propose that Azoarcus sp. strain T anaerobically oxidizes m-xylene to 3-methylbenzoate (or its CoA thioester) via (3-methylbenzyl)succinate and E-(3-methylphenyl)itaconate (or its CoA thioester) in a series of reactions that are analogous to those recently proposed for anaerobic toluene oxidation to benzoyl-CoA. A deuterium kinetic isotope effect was observed in the (3-methylbenzyl)succinate synthase reaction (and the benzylsuccinate synthase reaction), suggesting that a rate-determining step in this novel fumarate addition reaction involves breaking a C-H bond.  (+info)

Theoretical investigation of the [1,2]-sigmatropic hydrogen migration in the mechanism of oxidation of 2-aminobenzoyl-CoA by 2-aminobenzoyl-CoA monooxygenase/reductase. (2/69)

The flavin hydroperoxide at the active site of the mixed-function oxidase 2-aminobenzoyl-CoA monooxygenase/reductase (Azoarcus evansii) transfers an oxygen to the 5-position of the 2-aminobenzoyl-CoA substrate to provide the alkoxide intermediate II(-). Hydrogen migration from C5 to C6 follows this monooxygenation. The nature of the monooxygenation intermediate and plausible competing reactions leading to hydrogen migration have been considered. Ab initio molecular orbital theory has been used to calculate structures and electron distributions in intermediate and transition state structures. Electrostatic potential surface calculations establish that the transition state and product, associated with the C5 to C6 hydrogen transfer, are stabilized by electron distribution to the benzoyl-CoA thioester carbonyl oxygen. This is not so for the transition state and product associated with hydrogen transfer from C5 to C4. The activation energy for the 5, 6-shift is 2.5 kcal/mol lower than that for the 5,4-shift. In addition, the product of the hydrogen 5,6-shift is more stable than is the product of the hydrogen 5,4-shift, by approximately 6 kcal/mol. These results explain why only the shift of hydrogen from C5 to C6 is observed experimentally. Oxygen transfer and hydrogen migration almost coincide in the gas phase (activation energy of approximately 0.6 kcal/mol, equivalent to a single bond vibration). Enzymatic formation of alkoxide II(-) requires its stabilization; thus, the rate constant for its breakdown would be slower than in the gas phase.  (+info)

Biochemical and molecular characterization of phenylacetate-coenzyme A ligase, an enzyme catalyzing the first step in aerobic metabolism of phenylacetic acid in Azoarcus evansii. (3/69)

Phenylacetate-coenzyme A ligase (PA-CoA ligase; AMP forming, EC 6.2. 1.30), the enzyme catalyzing the first step in the aerobic degradation of phenylacetate (PA) in Azoarcus evansii, has been purified and characterized. The gene (paaK) coding for this enzyme was cloned and sequenced. The enzyme catalyzes the reaction of PA with CoA and MgATP to yield phenylacetyl-CoA (PACoA) plus AMP plus PPi. The enzyme was specifically induced after aerobic growth in a chemically defined medium containing PA or phenylalanine (Phe) as the sole carbon source. Growth with 4-hydroxyphenylacetate, benzoate, adipate, or acetate did not induce the synthesis of this enzyme. This enzymatic activity was detected very early in the exponential phase of growth, and a maximal specific activity of 76 nmol min(-1) mg of cell protein(-1) was measured. After 117-fold purification to homogeneity, a specific activity of 48 micromol min(-1) mg of protein(-1) was achieved with a turnover number (catalytic constant) of 40 s(-1). The protein is a monomer of 52 kDa and shows high specificity towards PA; other aromatic or aliphatic acids were not used as substrates. The apparent K(m) values for PA, ATP, and CoA were 14, 60, and 45 microM, respectively. The PA-CoA ligase has an optimum pH of 8 to 8.5 and a pI of 6.3. The enzyme is labile and requires the presence of glycerol for stabilization. The N-terminal amino acid sequence of the purified protein showed no homology with other reported PA-CoA ligases. The gene encoding this enzyme is 1, 320 bp long and codes for a protein of 48.75 kDa (440 amino acids) which shows high similarity with other reported PA-CoA ligases. An amino acid consensus for an AMP binding motif (VX2SSGTTGXP) was identified. The biochemical and molecular characteristics of this enzyme are quite different from those of the isoenzyme catalyzing the same reaction under anaerobic conditions in the same bacterium.  (+info)

Characterization of the Azoarcus ribozyme: tight binding to guanosine and substrate by an unusually small group I ribozyme. (4/69)

We report novel chemical properties of the ribozyme derived from the smallest group I intron (subgroup IC3) that comes from the pre-tRNA(Ile) of the bacterium Azoarcus sp. BH72. Despite the small size of the Azoarcus ribozyme (195 nucleotides (nt)), it binds tightly to the guanosine nucleophile (Kd = 15 +/- 3 microM) and exhibits activity at high temperatures (approximately 60-70 degrees C). These features may be due to the two GA3 tetraloop interactions postulated in the intron and the high GC content of the secondary structure. The second order rate constant for the Azoarcus ribozyme, ((k(cat)/Km)S = 8.4 +/- 2.1 x 10(-5) M(-1) min(-1)) is close to that found for the related ribozyme derived from the pre-tRNA(Ile) of the cyanobacterium Anabaena PCC7120. pH dependence studies and kinetic analyses of deoxy-substituted substrates suggest that the chemical cleavage step is the rate-determining process in the Azoarcus ribozyme. This may be due to the short 3-nt guide sequence-substrate pairing present in the Azoarcus ribozyme. Finally, the Azoarcus ribozyme shares features conserved in other group I ribozymes including the pH profile, the stereospecificity for the Rp-phosphorothioate at the cleavage site and the 1000-fold decrease in cleavage rate with a deoxyribonucleoside leaving group.  (+info)

Isolation and characterization of a new denitrifying spirillum capable of anaerobic degradation of phenol. (5/69)

Two kinds of phenol-degrading denitrifying bacteria, Azoarcus sp. strain CC-11 and spiral bacterial strain CC-26, were isolated from the same enrichment culture after 1 and 3 years of incubation, respectively. Both strains required ferrous ions for growth, but strain CC-26 grew better than strain CC-11 grew under iron-limited conditions, which may have resulted in the observed change in the phenol-degrading bacteria during the enrichment process. Strain CC-26 grew on phenol, benzoate, and other aromatic compounds under denitrifying conditions. Phylogenetic analysis of 16S ribosomal DNA sequences revealed that this strain is most closely related to a Magnetospirillum sp., a member of the alpha subclass of the class Proteobacteria, and is the first strain of a denitrifying aromatic compound-degrading bacterium belonging to this group. Unlike previously described Magnetospirillum strains, however, this strain did not exhibit magnetotaxis. It grew on phenol only under denitrifying conditions. Other substrates, such as acetate, supported aerobic growth, and the strain exhibited microaerophilic features.  (+info)

Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato and description of three new genera and new species, Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov. (6/69)

The taxonomic structure of members of the genus Azoarcus sensu lato was reassessed in a polyphasic approach. Two species, Azoarcus communis and Azoarcus indigens, three unnamed species containing diazotrophs associated with Kallar grass roots (groups C, D) and a group of strains (E) isolated from fungi were analysed. They were compared by PAGE analyses of cellular proteins, genomic fingerprints, morphological and nutritional features to new isolates from rice roots. All strains within groups C, D and E containing 5-12 isolates showed group-specific cell and colony morphology and carbon source utilization patterns, with exception of the obligately microaerobic strain BS20-3, a member of group C. All strains, with this exception, also had almost indistinguishable electrophoretic protein patterns and genomic fingerprints generated with tDNA-directed primers, suggesting they belong to the same species. Phylogenetic analyses of almost complete 16S rDNA sequences carried out with three different algorithms (neighbour-joining, maximum-likelihood, parsimony) revealed that Azoarcus sensu lato is not monophyletic. Groups C, D and E formed three distinct lineages located between the Azoarcus/Thauera and the Rhodocyclus clusters. Phylogenetic distances between groups C, D and E were as large as between other genera (93-94% sequence similarity). This suggested they have the rank of three different genera. Since it was possible to differentiate them from each other and other related bacteria by phenotypic features, three new genera with one type species each are proposed: Azovibrio restrictus gen. nov., sp. nov., Azospira oryzae gen. nov., sp. nov. and Azonexus fungiphilus gen. nov., sp. nov.  (+info)

Structure-function relationships of two closely related group IC3 intron ribozymes from Azoarcus and Synechococcus pre-tRNA. (7/69)

The two group IC3 pre-tRNA introns from Azoarcus and Synechococcus share very analogous secondary structures. They are small group I ribozymes that possess only two peripheral domains, P2 and P9. However, the 3'-splice site hydrolysis activity of the Synechococcus ribozyme critically depends on P2 whereas that of Azoarcus does not, indicating that the structure-function relationships of the two ribozymes are strikingly different despite their structural resemblance. To identify the element(s) that determines the catalytic properties of these ribozymes, we undertook analyses of chimeric ribozymes prepared by swapping their structural elements. We found that the difference can be attributed to a small number of nucleotides within the conserved core region. Further analysis by employing in vitro selection revealed that a base triple interaction (P4bp3 x J6/7-2) is a critical element for determining activity and suggests the existence of a novel base quintuple involving the base triple P4bp5 x J8/7-5.  (+info)

Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii. (8/69)

The aerobic metabolism of benzoate in the proteobacterium Azoarcus evansii was reinvestigated. The known pathways leading to catechol or protocatechuate do not operate in this bacterium. The presumed degradation via 3-hydroxybenzoyl-coenzyme A (CoA) and gentisate could not be confirmed. The first committed step is the activation of benzoate to benzoyl-CoA by a specifically induced benzoate-CoA ligase (AMP forming). This enzyme was purified and shown to differ from an isoenzyme catalyzing the same reaction under anaerobic conditions. The second step postulated involves the hydroxylation of benzoyl-CoA to a so far unknown product by a novel benzoyl-CoA oxygenase, presumably a multicomponent enzyme system. An iron-sulfur flavoprotein, which may be a component of this system, was purified and characterized. The homodimeric enzyme had a native molecular mass of 98 kDa as determined by gel filtration and contained 0.72 mol flavin adenine dinucleotide (FAD), 10.4 to 18.4 mol of Fe, and 13.3 to 17.9 mol of acid-labile sulfur per mol of native protein, depending on the method of protein determination. This benzoate-induced enzyme catalyzed a benzoyl-CoA-, FAD-, and O2-dependent NADPH oxidation surprisingly without hydroxylation of the aromatic ring; however, H2O2 was formed. The gene (boxA, for benzoate oxidation) coding for this protein was cloned and sequenced. It coded for a protein of 46 kDa with two amino acid consensus sequences for two [4Fe-4S] centers at the N terminus. The deduced amino acid sequence showed homology with subunits of ferredoxin-NADP reductase, nitric oxide synthase, NADPH-cytochrome P450 reductase, and phenol hydroxylase. Upstream of the boxA gene, another gene, boxB, encoding a protein of 55 kDa was found. The boxB gene exhibited homology to open reading frames in various other bacteria which code for components of a putative aerobic phenylacetyl-CoA oxidizing system. The boxB gene product was one of at least five proteins induced when A. evansii was grown on benzoate.  (+info)

Krause, A., H. Julich, M. Mankar. and B.Reinhold-Hurek, (2017) The Regulatory Network Controlling Ethanol-Induced Expression of Alcohol Dehydrogenase in the Endophyte Azoarcus sp. Strain BH72. Mol Plant-Microbe Interact 30: DOI:doi.org/10.1094/MPMI-01-17-0013-R. Sarkar, A., M. Marszalkowska, M. Schäfer, T. Pees, H. Klingenberg, F. Macht & B. Reinhold-Hurek, (2016) Global expression analysis of the response to microaerobiosis reveals an important cue for endophytic establishment of Azoarcus sp. BH72. Environ Microbiol 19: 198-217.. Grönemeyer, J.L., T. Hurek & B. Reinhold-Hurek, (2015) Bradyrhizobium kavangense sp. nov., a symbiotic nitrogen-fixing bacterium from root nodules of traditional Namibian pulses. Int. J. Syst. Evol. Microbiol. 65: 4886-4894.. Reinhold-Hurek, B., W. Bünger, C.S. Burbano, M. Sabale & T. Hurek, (2015) Roots shaping their microbiome: Global hot spots for microbial activity. Annu. Rev. Phytopathol. 53: 403-424.. Burbano, C.S., J.L. Grönemeyer, T. Hurek & B. ...
Ray, S. K., Rajeshwari, R., Shar ma, Y., and Sonti, R. V. 2002. A high-molecular-weight outer membrane protein of Xanthomonas oryzae pv. oryzae exhibits similarity to nonfimbrial adhesins of animal pathogeni c bacteria and is required for optimum virulence. Mol. Microbiol. 46:637-647. Regu, M., Enfedaque, J., Camprubi, S., and Toms, J. M. 1992. The O-antigen lipopolysaccharide is the major barrier to plasmid DNA uptake by Klebsiella pneumoniae during transformation by electroporation and os motic shock. J. Microbiol. Methods 15:129-134. Reinhold-Hurek, B., Maes, T., Gemmer, S., Van Montagu, M., and Hurek, T. 2006. An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp strain BH72. Mol. Plan t-Microbe Interact. 19:181-188. Reiter, B., Brgmann, H., Burg, K., and Sessitsch, A. 2003. Endophytic nifH gene diversity in African sweet potato. Can. J. Microbiol. 49:549-555. Reslewic, S., Zhou, S., Place, M., Zhang, Y. P., Briska, A., Goldstein, S., ...
Azoarcus sp. strain BH72 is a mutualistic N2-fixing endophyte of salt-tolerant Kallar grass (Leptochloa fusca (L.) Kunth), a grass gowing in the Punjab of Pakistan. Endophytes are bacteria that live within the tissues of plants without causing them any harm; Azoarcus resides within the roots of its host. Azoarcus sp. strain BH72 is also capable of endophytic N2 fixation in rice and sugarcane. The bacteria has not been detected in root-free soil, although it is able to be cultured. It does not however excrete significant amounts of nitrogenous compounds in culture. The genome sequence has shown that Azoarcus has very few mobile genetic elements, or phage-related genes, suggesting its adaptation to a fairly stable, low-stress environment. The sec-dependent, SRP-mediated and Tat systems for protein secretion are all present, in addition to a type I, the type IIb secretion systems and an autotransporter. However it is lacking both type III and type IV secretion systems, suggesting it can not export ...
The enzyme is involved in the aerobic benzoyl-CoA catabolic pathway of the bacterium Azoarcus evansii. The enzyme converts 2,3-epoxy-2,3-dihydrobenzoyl-CoA to its oxepin form prior to the ring-opening and the formation of a dialdehyde intermediate ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Nonhomologous end joining (NHEJ) eliminates DNA double-strand breaks (DSBs) by direct ligation. NHEJ involves binding of the KU heterodimer to double-stranded DNA ends, recruitment of DNA-PKcs (MRX complex in yeast), processing of ends, and recruitment of the DNA ligase IV (LIG4)-XRCC4 complex, which brings about ligation. A recent study shows that bacteria accomplish NHEJ using just two proteins (Ku and DNA ligase), whereas eukaryotes require many factors. NHEJ repairs DSBs at all stages of the cell cycle, bringing about the ligation of two DNA DSBs without the need for sequence homology, and so is error-prone ...
SWISS-MODEL Repository entry for A1KAA5 (PANB_AZOSB), 3-methyl-2-oxobutanoate hydroxymethyltransferase. Azoarcus sp (strain BH72)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Advocacy is the act of educating, supporting and recommending an action. At the Cutaneous Lymphoma Foundation, we advocate on behalf of people affected by cutaneous lymphomas to affect positive change on many important fronts.
Azoarcus tolulyticus is a new class of widely distributed toluene-degrading denitrifiers of potential importance in remediating benzene, toluene, ethylbenzene, and xylene (BTEX)-contaminated environments. To detect these organisms in the environment, 16S rRNA gene-based phylogenetic probes were developed. Two sets of specific PCR amplification primers and two oligonucleotide hybridization probes were designed and tested against both closely and distantly related environmental isolates. All of these primers and probes were specific to the species A. tolulyticus. The sensitivity of the PCR amplification primer sets was evaluated with DNA isolated from A. tolulyticus Tol-4 pure culture and from sterile soils seeded with a known number of Tol-4 and Escherichia coli cells. These primer sets were able to detect 1 fg to 1 pg of template DNA from the pure culture and 1.11 x 10(2) to 1.1 x 10(8) Tol-4 cells per g of soil in the presence of 1.56 x 10(10) E. coli cells. These two PCR amplification primers ...
Activation of benzylsuccinate synthase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5-deoxy-adenosine.
Peptide-bond VUV absorption is inherent to all proteins and peptides. Although widely exploited in top-down proteomics for photodissociation, this absorption has never been spectroscopically characterized in the gas phase. We have measured VUV/UV photofragmentation spectrum of a single peptide bond in a cryogenically cold protonated dipeptide. Although the spectrum appears to be very broadband and structureless, vibrational pre-excitation of this and even larger cold peptides significantly increases the UV dissociation yield for some of their photofragments. We use this effect to extend the technique of IR−UV photofragmentation vibrational spectroscopy, developed for aromatic peptides, to nonaromatic ones and demonstrate measurements of conformation-specific and nonspecific IR spectra for di- to hexa-peptides. Pereverzev, Aleksandr Y.; Kopysov, Vladimir N.; Boyarkin, Oleg V.
The first stage, glycolysis is the anaerobic catabolism of glucose, it occurs in almost all cells. The process uses glucose and co-enzyme NAD (Nicotinamide Adenine Dinucleotide), and yields 2 molecules of Pyruvic acid, as below C6H12O6 + 2NAD+ -, 2C3H4O3 + 2NADH + 2H+ The free energy stored in 2 molecules of pyruvic acid is somewhat less than that in the original glucose molecule. Some of this difference is captured in 2 molecules of ATP. The Krebs Cycle then decarboxylates the pyruvic acid resulting in a 2-carbon fragment of acetate. This 2-carbon fragment is coupled to a molecule of oxaloacetic acid , this results in a molecule of citric acid (the process is also known as the citric acid cycle) ...
SOLUTION STRUCTURE OF A 22-NUCLEOTIDE HAIRPIN SIMILAR TO THE P5ABC REGION OF A GROUP I RIBOZYME WITH COBALT(III)HEXAMMINE COMPLEXED TO THE GAAA TETRALOOP. ...
RNA. 2011 Nov;17(11):1967-72. doi: 10.1261/rna.2757311. Epub 2011 Sep 29. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
These pumps disappeared in excised root segments. In excised root tips, there appeared to be weak K+ pumps at both sites in the ...
PK 11195 | BZD antagonist | PK11195 | CAS [85532-75-8] | Axon 1208 | Axon Ligand™ with >99% purity available from supplier Axon Medchem, prime source of life science reagents for your research
Plasmid CIB81-pmGFP from Dr. Chandra Tuckers lab contains the insert CIB1 and is published in Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. This plasmid is available through Addgene.
prephenic acid: nonaromatic biosynthetic intermediate, probable immediate precursor of aromatic systems such as phenylalanine, tyrosine, tannins, flavanoids; isolated from cultures of mutant E coli; structure
We next selected several positions based on their close proximity and direct atomic interactions with EVT-101. Disruption of the key π-stacking interaction with the central pyridazine ring of EVT-101 by mutating GluN1-Y109 into the nonaromatic and short serine resulted in a drastic decrease in EVT-101 sensitivity (800-fold increase in IC50). In contrast, sensitivity to ifenprodil was barely affected (,2-fold change in IC50; Fig. 3B, and see also the GluN1-Y109C mutation in Table 1), thus providing strong evidence for the critical importance of residue GluN1-Y109 in mediating EVT-101 inhibitory action. We obtained additional support for the relevance of the EVT-101 binding site by investigating mutations at positions GluN2B-Q110 (α2 helix) and GluN2B-A135 (β4-β5 loop). As described above, both residues line the EVT-101 cavity, making specific contacts with the ligand. Substituting GluN2B-Q110 by a glycine to avoid any side chain-ligand interaction strongly decreased EVT-101 sensitivity ...
किस प्रकार की क्षति होती है:. 1. युवा और वयस्क कीट कोमल जड़ों से रस चूस लेते हैं। गंभीर आक्रमण होने की स्थिति में अंकुर की वृद्धि रूक जाती है, रंग फीका पीला हो जाता है और उसमें फूल नहीं लगते हैं।. 2. बुआई के 25-30 दिनों के बाद वानस्पतिक अवस्था (वेजिटेटिव स्टेज़) में आक्रमण प्रारंभ होकर फसल के पकने तक बना रहता है।. चावल की जड़ों में पाए जाने वाले कीड़ों (राइस रूट एफिड) का प्रबंधन (Management of Rice root aphid ). 1. ...
Microbiology students of [http://www.biology.ucsd.edu/classes/bimm120.SP07/ Kit Pogliano and Rachel Larsen] at the University of California at San Diego are currently authoring the following pages as term projects for their course, BIMM120 Bacteriology.,br> ,table border=0 cellpadding=2 cellspacing=2 width=100%> ,tr valign=top> ,td> [[Acinetobacter baumannii]],br> By Jennifer Wang [[Actinobacillus pleuropneumoniae]],br> By Nhu Le [[Agrobacterium tumefaciens]],br> By Allan Wu [[Alcanivorax borkumensis]],br> By Justin Shih [[Anaeromyxobacter dehalogenans]],br> By Lori Nacario [[Anaplasma marginale]],br> By Patricia Shih [[Archaeoglobus fulgidus]],br> By Mike Sha [[Azoarcus sp. strain BH72]],br> By Caine Ballew [[Bacillus anthracis]], causes anthrax By Grace Ucar [[Bacillus clausii]],br> By Ankur Pate [[Bacillus licheniformis]],br> By Thu Mai [[Bacillus ...
Disclosed are polymeric dyes of formula (1a), (1b), or (1c); wherein A and B, independently from each other represent a polymer backbone; X1 and X2 independently from each other are a linkage group selected from --S--, --S--S--, --N--, --N═--, --N(R5)--, --S(O)--, --SO2--, --(CH2CH2--O)1-5--, --(CH2CH2CH2--O)1-5--, --C(O)--, --C(O)O--, --OCO--, (formula II) --CON(R1)--, --C(NR1R2)2--, --(R1)NC(O)--, --C(S)R1--; which may be interrupted and/or terminated at one or both ends by one or more than one --C1C30alkylene- or --C2-C12alkenylene-; or an optionally substituted, saturated or unsaturated, fused or non-fused aromatic or nonaromatic (heterocyclic) bivalent radical optionally comprising at least one heteroatom; a saturated or unsaturated, fused or non-fused aromatic or nonaromatic bivalent radical comprising at least one heteroatom, which is optionally substituted by C1-C30alkyl, C1-C30alkoxy, C2-C12alkenyl, C5-C10aryl, C5-C10cycloalkyl, C1-C10alkyl(C5-C10arylene), hydroxy or halogen; R1 and ...
The April 2014 edition of the New Journal of Botany (Volume 4 Number 1) contains the following article: British Sorbus (Rosaceae): six new species, two hybrids and a new subgenus by T. C. G. Rich, D. Green, L. Houston, M. Lepší, S. Ludwig and J. Pellicer.. The new species are Evanss Whitebeam (Sorbus evansii), Greens Whitebeam (S. greenii), Herefordshire Whitebeam (S. herefordensis), Richs Whitebeam (S. richii), Sells Whitebeam (S. sellii) and Observatory Whitebeam (S. spectans). These all grow around the Severn and Severn estuary and the article gives maps and details of locations with detailed descriptions of the species should anyone want to go and see them.. The new hybrids are Sorbus aucuparia x S. minima where a single example has been found in the Brecon Beacons and Sorbus porrigentiformis x. S. torminalis with two trees on a cliff in Monmouthshire.. All these species and hybrids have been defined as distinct using genetic analysis techniques.. The new subgenus is Triparens, set up ...
The wide, but scattered distribution of group I introns in nature is a result of two processes; the vertical inheritance of introns with or without losses, and the occasional transfer of introns across species barriers. Reversal of the group I intron
Novel Group 4, Group 3 or Lanthanide metal complexes wherein the metal is in the +3 formal oxidation state containing a cyclic or non-cyclic, nonaromatic, anionic, dienyl ligand group bound to M and having a bridged ligand structure, catalytic derivatives of such complexes; and the use thereof as catalysts for polymerizing addition polymerizable monomers are disclosed.
Dortmund Data bank - Thermophysical Properties | Benzene, CAS Number: 71-43-2 | m-Xylene, CAS Number: 108-38-3 | SpringerMaterials 2014
EC number: 297-401-8 , CAS number: 93571-75-6 A complex combination of hydrocarbons obtained by separation from the platformate-containing fraction. It consists predominantly of aromatic hydrocarbons having carbon numbers predominantly in the range of C7 through C12 (primarily C8) and can contain nonaromatic hydrocarbons, both boiling in the range of approximately 130°C to 200°C (266°F to 392°F). ...
Kink definition is - a short tight twist or curl caused by a doubling or winding of something upon itself. How to use kink in a sentence.
Transcription of E. coli SRP RNA by E. coli polymerase is characterized by prominent pauses at residues U82 and U84 (Fig. 3 A). These pause sites occur just upstream to the 3′ portions of its two long-range helices. Both sites can be tentatively assigned as type I hairpin-dependent pause sites (10). Among γ proteobacteria, 72% have the first, whereas 86% have the second 5′UG signature sequence at this location (Fig. 3 A, graph).. Because a functional in vitro folding assay for SRP RNA is unavailable, oligo-probes complementary to the regions encompassing the 5′ and the 3′ portions of these two long range helices were used to assay folding (Fig. 3 B). Similar assays have been used extensively in folding studies of the group I ribozyme (30). In wild-type polymerase transcription, the extent of protection increased over time as more SRP RNA molecules presumably folded into their native structures (SI Fig. 8). Both pause sites were absent when transcription was performed by using the same ...
A novel organocatalyst was developed that effectively catalyzed the reactions of unprotected or protected dihydroxyacetone with a variety of aldehydes to provide syn-aldol products with good yields and ee values up to >99%. Significantly, this amide catalyst was effective with a variety of nonaromatic aldehyde acceptors that had proven difficult in the presence of other catalysts. Reactions of protected dihydroxyacetone proceeded in aqueous media without addition of organic solvents ...
Glucosepane is a structurally complex protein posttranslational modification that is believed to exist in all living organisms. Research in humans suggests that glucosepane plays a critical role in the pathophysiology of both diabetes and human aging, yet comprehensive biological investigations of this metabolite have been hindered by a scarcity of chemically homogeneous material available for study. Here we report the total synthesis of glucosepane, enabled by the development of a one-pot method for preparation of the nonaromatic 4H-imidazole tautomer in the core. Our synthesis is concise (eight steps starting from commercial materials), convergent, high-yielding (12% overall), and enantioselective. We expect that these results will prove useful in the art and practice of heterocyclic chemistry and beneficial for the study of glucosepane and its role in human health and disease. ...
acenol, acerol, acetarsol, acmiol, acyloglicerol, Adampol, adinol, Adrianopol, aerosol, aerozol, akropol, Akropol, akseroftol, aldol, ALGOL, Algol, alizarol, alkohol, alumatol, amatol, amfibol, amidol, aminoalkohol, aminofenol, amol, Anatol, Andrespol, anetol, angol, Angol, Annopol, anticol, antikol, antresol, antymetabol, AOL, apiol, apsydiol, archeol, atol, babol, banderol, barkarol, bazgrol, bejsbol, bemol, benzohol, benzol, benzopirol, biadol, Bibiampol, bioetanol, biometanol, bisabolol, bombykol, borazol, borneol, bristol, Bristol, brizol, Brol, bromofenol, brystol, bryzol, bucol, busol, butanol, butapirazol, bzdęgol, bzdręgol, Capitol, carmagnol, carol, centriol, certol, Chabrol, chinoksyzol, chinol, Chinol, chinozol, chloramfenikol, cholekalcyferol, cholesterol, chromol, chryzokol, cibazol, COBOL, Cobol, Colargol, control, Control, cool, cykloheksanol, cyneol, cytronelol, czebol, dehydrocholesterol, dehydroretinol, dekapol, Dekapol, dermatol, diabetol, dikumarol, dimerkaprol, dipol, ...
acenol, acerol, acetarsol, acmiol, acyloglicerol, Adampol, adinol, Adrianopol, aerosol, aerozol, akropol, Akropol, akseroftol, aldol, ALGOL, Algol, alizarol, alkohol, alumatol, amatol, amfibol, amidol, aminoalkohol, aminofenol, amol, Anatol, Andrespol, anetol, angol, Angol, Annopol, anticol, antikol, antresol, antymetabol, AOL, apiol, apsydiol, archeol, atol, babol, banderol, barkarol, bazgrol, bejsbol, bemol, benzohol, benzol, benzopirol, biadol, Bibiampol, bioetanol, biometanol, bisabolol, bombykol, borazol, borneol, bristol, Bristol, brizol, Brol, bromofenol, brystol, bryzol, bucol, busol, butanol, butapirazol, bzdęgol, bzdręgol, Capitol, carmagnol, carol, centriol, certol, Chabrol, chinoksyzol, chinol, Chinol, chinozol, chloramfenikol, cholekalcyferol, cholesterol, chromol, chryzokol, cibazol, COBOL, Cobol, Colargol, control, Control, cool, cykloheksanol, cyneol, cytronelol, czebol, dehydrocholesterol, dehydroretinol, dekapol, Dekapol, dermatol, diabetol, dikumarol, dimerkaprol, dipol, ...
Cyanothece sp. ATCC ® 51142D-5™ Designation: Genomic DNA from Cyanothece sp. strain BH68 TypeStrain=False Application:
Cyanothece sp. ATCC ® 51142D-5™ Designation: Genomic DNA from Cyanothece sp. strain BH68 TypeStrain=False Application:
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View Notes - Lecture8 from BIOC 100A at UCSC. 25 26 The hammerhead ribozyme (plant virus) Martick & Scott, Cell 2006 27 Group I intron ribozyme Golden et al, and cech Science (1998) 28 Acid-Base
Description. The internationally acclaimed author of the L.A. Quartet and The Underworld USA Trilogy, James Ellroy, presents another literary noir masterpiece of historical paranoia. A rip-roaring, devilishly wild ride through the bloody end of the 1960s. Its dark baby, and hot hot hot. Martin Luther King assassinated. Robert Kennedy assassinated. Los Angeles, 1968. Conspiracies theories are taking hold. On the horizon looms the Democratic Convention in Chicago and constant gun fire peppers south L.A. Violence, greed, and grime, are replacing free-love and everybody from Howard Hughes, Richard Nixon and J. Edgar Hoover to the right-wing assassins and left-wing revolutionaries are getting dirty. At the center of it all is a triumvirate: the presidents strong-arm goon, an ex-cop and heroine runner, and a private eye whose quarry is so dangerous she could set off the whole powder keg. With his trademark deadly staccato prose, James Ellroy holds nothing back in this wild, startling and much ...
nov., and Azoarcus buckelii sp. nov". Arch Microbiol. 178: 26-35. doi:10.1007/s00203-002-0422-6. PMID 12070766. UniProt Type ...
nov., and Azoarcus, as Azoarcus evansii sp. nov., Respectively, Members of the Beta Subclass of the Proteobacteria". ...
Kniemeyer, O.; Heider, J. (2001). "(S)-1-phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ...
... and is officially it is referred to Azoarcus sp. EbN1 as it belongs to the Azoarcus/Thauera cluster. The discovery of the ...
3-dioxygenase is involved in aerobic benzoate metabolism in Azoarcus evansii. Zaar, A.; Gescher, J.; Eisenreich, W.; Bacher, A ... "Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii". J. Bacteriol. 184 (22): 6301-6315. doi: ... "Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii". J. Bacteriol. 183 (6): ... Fuchs, G. (2004). "New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii". Mol. Microbiol. 54 (1): 223-238. ...
Uliginosibacterium and Azoarcus. Cells are rod shaped. Dominant respiratory quinones are ubiquinone-8 and rhodoquinone-8. G+C ...
Harder, J. (1997). "Anaerobic degradation of cyclohexane-1,2-diol by a new Azoarcus species". Arch. Microbiol. 168: 199-204. ...
Gescher, J.; Eisenreich, W.; Worth, J.; Bacher, A.; Fuchs, G. (2005). "Aerobic benzoyl-CoA catabolic pathway in Azoarcus ... formate The enzyme is involved in the aerobic benzoyl-CoA catabolic pathway in Azoarcus evansii. ...
... a new enzyme of anaerobic phenylalanine metabolism in the denitrifying bacterium Azoarcus evansii". Eur. J. Biochem. 251 (3): ...
... a denitrifying bacterium related to the genera Azoarcus and Thauera. It is a molybdenum enzyme belonging to the DMSO reductase ...
... by the denitrifying bacterium Azoarcus anaerobius". Journal of Bacteriology. 180 (14): 3644-9. PMC 107334 . PMID 9658009. ...
... catabolic pathway in Azoarcus evansii: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase ... H+ This enzyme catalyses a step in the aerobic benzoyl-coenzyme A catabolic pathway in Azoarcus evansii and Burkholderia ...
used an Azoarcus group I intron ribozyme that, when fragmented, has an ability to self-assemble by catalysing recombination ... akin to a recombination of Azoarcus ribozyme from four inactive oligonucleotide fragments described earlier. Apart from a ...
Reinhold-Hurek, B.; Hurek, T. (2000). "Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato ...
Reinhold-Hurek, B.; Hurek, T. (2000). "Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato ...
Reinhold-Hurek, B.; Hurek, T. (2000). "Reassessment of the taxonomic structure of the diazotrophic genus Azoarcus sensu lato ...
Strobel's group solved the x-ray crystal structure of the full length Azoarcus Group I catalytic intron (PDB entries 1U6B, 1ZZN ...
... which was unambiguously proven by a recently resolved high-resolution structure of the Azoarcus group I intron. Since the early ...
Azoarcus MeSH B03.660.075.655.800 --- Thauera MeSH B03.660.075.655.975 --- Zoogloea MeSH B03.660.075.710 --- Spirillaceae MeSH ... Azoarcus MeSH B03.440.450.340 --- Capnocytophaga MeSH B03.440.450.342 --- cardiobacteriaceae MeSH B03.440.450.342.100 --- ...
... indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus is a genus of nitrogen-fixating bacteria. Species in this genus are usually found in contaminated water, as they are ... ISBN 0-7923-4514-2. LPSN Azoarcus at the Encyclopedia of Life. ... Azoarcus spp., by analyses of partial 16S ribosomal DNA ... "Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and ...
... is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are Gram-negative, motile and rod- ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... ISBN 0-7923-4514-2. LPSN Type strain of Azoarcus olearius at BacDive - the Bacterial Diversity Metadatabase. ... "Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil". International Journal of ...
... is a bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus toluvorans Song, B; ... and Azoarcus toluclasticus sp. nov". Int J Syst Bacteriol. 49 Pt 3: 1129-40. doi:10.1099/00207713-49-3-1129. PMID 10425771. ... Type strain of Azoarcus toluvorans at BacDive - the Bacterial Diversity Metadatabase. ... "Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. ...
... is a gram negative bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus ... and Azoarcus toluclasticus sp. nov". Int J Syst Bacteriol. 49 Pt 3: 1129-40. doi:10.1099/00207713-49-3-1129. PMID 10425771. ... "Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. ...
LSPN bacterio.net Straininfo of Azoarcus anaerobius Springer, N; Ludwig, W; Philipp, B; Schink, B (1998). "Azoarcus anaerobius ... Azoarcus anaerobius is a gram-negative, strictly anaerobic, catalase-negative, nitrate-reducing, rod-shaped bacterium from the ... UniProt International Journal of Systematic Bacteriology [1] Type strain of Azoarcus anaerobius at BacDive - the Bacterial ...
ISBN 0-7923-4514-2. Azoarcus communis at the Encyclopedia of Life LPSN Type strain of Azoarcus communis at BacDive - the ... Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus communis is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are yellow-pigmented, straight to ... Reinhold-Hurek, B.; Hurek, T.; Gillis, M.; Hoste, B.; Vancanneyt, M.; Kersters, K.; De Ley, J. (1993). "Azoarcus gen. nov., ...
... at the Encyclopedia of Life LPSN Type strain of Azoarcus indigens at BacDive - the Bacterial Diversity ... Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus indigens is a species of bacteria. It is a nitrogen-fixing bacteria associated with roots of Leptochloa fusca . Its ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ...
... is a bacterium from the genus of Azoarcus. LSPN bacterio.net Straininfo of Azoarcus buckelii The Taxonomicon ... nov., and Azoarcus buckelii sp. nov". Arch Microbiol. 178: 26-35. doi:10.1007/s00203-002-0422-6. PMID 12070766. Type strain of ... Azoarcus buckelii at BacDive - the Bacterial Diversity Metadatabase. ...
... is a species of bacteria. Its type strain is KB 740T. This particular strain of Azoarcus evansii has been ... Azoarcus evansii at the Encyclopedia of Life Type strain of Azoarcus evansii at BacDive - the Bacterial Diversity Metadatabase ... nov., and Azoarcus, as Azoarcus evansii sp. nov., Respectively, Members of the Beta Subclass of the Proteobacteria". ... The Azoarcus evansii consumes (literally eats) the Lactobacillus johnsonii to maintain a healthy population level. It has been ...
... is a species of bacteria. It is a nitrogen-fixing bacteria. It is notable for degrading toluene. Tol-4 is ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... LPSN Azoarcus tolulyticus at the Encyclopedia of Life. ... Azoarcus tolulyticus, with small-subunit rRNA primers and ... "Phylogenetic Analyses of a New Group of Denitrifiers Capable of Anaerobic Growth on Toluene and Description of Azoarcus ...
Non-homologous end-joining - Azoarcus sp. BH72 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Azoarcus sp. (strain BH72)Imported. ,p>Information which has been imported from another database using automatic procedures.,/p ... tr,A1K5G6,A1K5G6_AZOSB Putative citrate chemoreceptor protein OS=Azoarcus sp. (strain BH72) GN=tcp PE=4 SV=1 ...
A1KAA5 (PANB_AZOSB) Azoarcus sp (strain BH72). 3-methyl-2-oxobutanoate hydroxymethyltransferase UniProtKBInterProSTRING ...
Model system approach: Endophytic diazotrophic bacteria (Azoarcus spp. and related genera) and Gramineae such as rice and ... Proteomic characterization of a pilR regulatory mutant of Azoarcus sp. strain BH72 with the aid of gel-based and gel-free ... Azoarcus sp. strain BH72 interactions. Mol. Plant-Microbe Interact. 25: 191-199. ... Global expression analysis of the response to microaerobiosis reveals an important cue for endophytic establishment of Azoarcus ...
Azoarcus sp. strain BH72 is also capable of endophytic N2 fixation in rice and sugarcane. The bacteria has not been detected in ... Azoarcus sp. strain BH72 is a mutualistic N2-fixing endophyte of salt-tolerant Kallar grass (Leptochloa fusca (L.) Kunth), a ... Endophytes are bacteria that live within the tissues of plants without causing them any harm; Azoarcus resides within the roots ... The genome sequence has shown that Azoarcus has very few mobile genetic elements, or phage-related genes, suggesting its ...
Azoarcus indigens sp. nov. and Azoarcus communis sp. nov". International Journal of Systematic Bacteriology. 43 (3): 574-584. ... Azoarcus is a genus of nitrogen-fixating bacteria. Species in this genus are usually found in contaminated water, as they are ... ISBN 0-7923-4514-2. LPSN Azoarcus at the Encyclopedia of Life. ... Azoarcus spp., by analyses of partial 16S ribosomal DNA ... "Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and ...
Azoarcus olearius is a species of bacteria. It is a nitrogen-fixing bacteria. Its cells are Gram-negative, motile and rod- ... Hurek T, Reinhold-Hurek B (June 1995). "Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp., by ... ISBN 0-7923-4514-2. LPSN Type strain of Azoarcus olearius at BacDive - the Bacterial Diversity Metadatabase. ... "Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil". International Journal of ...
Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63T and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that DQS-4 ... was sequenced and compared with other Azoarcus strains. The genome sequence showed high synteny with Azoarcus sp. BH72, a model ... The genome of Azoarcus olearius DQS-4T , a N2 -fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, ... The oil-contaminated soil diazotroph Azoarcus olearius DQS-4T is genetically and phenotypically similar to the model grass ...
The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus ( ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ... and Azoarcus toluclasticus sp. nov.. Song B1, Häggblom MM, Zhou J, Tiedje JM, Palleroni NJ. ... A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second ...
Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus; unclassified Azoarcus. Data source. ... Azoarcus pumilus sp. nov., isolated from seawater in Sanya, China.. Journal. Int J Syst Evol Microbiol 69:1459-1464 (2019). DOI ...
The endophytic bacterium Azoarcus sp. strain BH72 is capable of colonizing the interior of rice roots, where it finds suitable ... The endophytic bacterium Azoarcus sp. strain BH72 is capable of colonizing the interior of rice roots, where it finds suitable ... Thus, it is likely that ethanol is an important carbon source for the endophytic life of Azoarcus sp. ... fusion library of Azoarcus sp. grown under different conditions was performed. Monitoring of the GFP activity allowed the ...
Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB.. Authors: Durante-Rodríguez, Gonzalo ... promoter responsible for the anaerobic catabolism of benzoate in Azoarcus sp. CIB constitutes the prototype of a new subfamily ...
Azoarcus sp. strain BH72 is an endophytic betaproteobacterium able to colonize rice roots without induction of visible disease ... Azoarcus sp. strain BH72 is an endophytic betaproteobacterium able to colonize rice roots without induction of visible disease ... the hypothesis that FliC3 does not play a major role as a MAMP but is required for endophytic colonization in the Azoarcus-rice ...
Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T.. Title. Substrate range of benzylsuccinate synthase ... In substrate range studies with partially purified benzylsuccinate synthase from denitrifying Azoarcus sp. strain T, ...
Azoarcus tolulyticus is a new class of widely distributed toluene-degrading denitrifiers of potential importance in remediating ... Sensitive detection of a novel class of toluene-degrading denitrifiers, Azoarcus tolulyticus, with small-subunit rRNA primers ... Sensitive detection of a novel class of toluene-degrading denitrifiers, Azoarcus tolulyticus, with small-subunit rRNA primers ... Sensitive detection of a novel class of toluene-degrading denitrifiers, Azoarcus tolulyticus, with small-subunit rRNA primers ...
E-Phenylitaconic acid has been isolated as a metabolite generated by Azoarcus tolulyticus Tol-4 along with benzylsuccinic acid ... and Azoarcus toluclasticus sp. nov.. *Binwei Song, Max M Häggblom, Jian-ping Zhou, James M. Tiedje, Norberto J. Palleroni ... Initial reactions in anaerobic oxidation of m-xylene by the denitrifying bacterium Azoarcus sp. strain T.. *Cynthia J. Krieger ... E-Phenylitaconic acid has been isolated as a metabolite generated by Azoarcus tolulyticus Tol-4 along with benzylsuccinic acid ...
The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus ( ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ... A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second ... Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In ...
Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of ... We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as ... Battistoni F, Bartels D, Kaiser O, Reamon-Buettner SM, Hurek T, Reinhold-Hurek B. Physical map of the Azoarcus sp strain BH72 ... Physical map of the Azoarcus sp strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome ...
Azoarcus sp. strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It ... Azoarcus sp. strain PA01,sup,T,/sup, belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class ... Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01,sup,T,/sup,. * Home ... Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. strain PA01,sup,T,/sup,. 2015. eng. ...
Plasmids were introduced into Azoarcus sp. strain T by electroporation. Briefly, cells of Azoarcus sp. strain T were grown ... The bssE gene of Azoarcus sp. T is predicted to start at A7499TG and end at T8357AA. BssE has a predicted molecular mass of ... Role of bssA in anaerobic growth on (A) toluene and (B) m-xylene in Azoarcus sp. strain T. Growth of the wild-type strain on ... Phenotype of a ΔbssA mutant. Azoarcus sp. strain T is able to grow anaerobically with either toluene or m-xylene as the sole ...
Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T journal, September 1999 * Beller, Harry R.; Spormann, ...
Correction: Corrigendum: Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 *Andrea Krause ... Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 *Andrea Krause ... Rights & permissionsfor article Complete genome of the mutualistic, N,sub,2,/sub,-fixing grass endophyte ,i,Azoarcus,/i, sp. ... Azoarcus,/i, sp. strain BH72 . Opens in a new window. ...
Azoarcus sp. (strain BH72). Loading... A1KFH3 Protein GrpE. Mycobacterium bovis (strain BCG / Pasteur 1173P2). Loading... ...
Azoarcus sp. (strain BH72). Loading... A1KKP9 Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase. ...
Organism azo: Azoarcus sp. BH72. to run JSim applets. See JSim Kegg models page for comlete list of Kegg models. ...
Azoarcus sp. BH72. Paenibacillus odorifer. Tuber harvested in Oct. 2002, and Oct. 2004 from cv. Beniazuma grown on an Andozol, ... Azoarcus sp. BH72. Clostridium pasteurianum. Stem harvested in Oct. 2002, Aug. 2004 and Oct. 2004 from cv. Beniazuma grown in ... β-Proteobacteria (Azoarcus sp., Azospira oryzae, Azotobacter sp.) γ-Proteobacteria (Methylococcus) δ-Proteobacteria (Geobacter ... and Azoarcus sp. BH72 (Table 6). The nifH DNA was also isolated from the stem and tuber samples from several-months-old sweet ...
DNA-DNA hybridization experiments established that strains 2FB2, 2FB6 and 4FB10 belong to the species Azoarcus tolulyticus. ... 2FB6 and 4FB10 as new members of the genus Azoarcus and strains 4FB1, 4FB2, 3CB2, 3CB3 and 3BB1 as new members of the genus ... The taxonomic relationships of Azoarcus and Thauera isolates in the beta-subclass of the Proteobacteria capable of degrading ... The taxonomic relationships of Azoarcus and Thauera isolates in the beta-subclass of the Proteobacteria capable of degrading ...
A1K601 (LEPA_AZOSB) Azoarcus sp (strain BH72). Elongation factor 4 UniProtKBInterProSTRINGInteractive Modelling. 598 aa; ...
strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It is a ... strain KH32C [23] and Azoarcus sp. strain BH72 [24] and Azoarcus toluclasticus ATCC 700605 [25]. The exact phylogenetic ... Azoarcus sp. strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It ... Azoarcus buckelii DSM 14744T (99 % gene similarity) [14, 15] and Azoarcus anaerobius (98 %) [16]. A. buckelii DSM 14744T was ...
strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It is a ... Azoarcus sp. strain PA01T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. It ... The closest neighbors of strain PA01T whose genome sequence is available are Azoarcus sp. strain KH32C [23] and Azoarcus sp. ... Graphical representation of different scaffolds of the genome of Azoarcus sp. strain PA01T. a Graphical map of Azoarcus sp. ...
Azoarcus sp. CIB. 97. UniRef50_A0A0K1J4X7. DUF2933 domain-containing protein. Proteobacteria bacterium ...
Symbiotic nitrogen fixers include the following genera, Rhizobium, Achromobacter, Sinorhizobium, Azoarcus, Mesorhizobium, ... Azoarcus sp., Herbaspirillum sp., Gluconacetobacter diazotrophicus, and Azotobacter sp. (Vessey, 2003). ...
  • and B.Reinhold-Hurek, (2017) The Regulatory Network Controlling Ethanol-Induced Expression of Alcohol Dehydrogenase in the Endophyte Azoarcus sp. (uni-bremen.de)
  • Endophytic diazotrophic bacteria ( Azoarcus spp . (uni-bremen.de)
  • Sarkar, A., M. Marszalkowska, M. Schäfer, T. Pees, H. Klingenberg, F. Macht & B. Reinhold-Hurek, (2016) Global expression analysis of the response to microaerobiosis reveals an important cue for endophytic establishment of Azoarcus sp. (uni-bremen.de)
  • BH72, a model endophytic diazotroph, but low synteny with five non-plant-associated strains (Azoarcus CIB, Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63 T and Azoarcus PA01). (nih.gov)
  • Average Nucleotide Identity (ANI) revealed that DQS-4 T shares 98.98% identity with Azoarcus BH72, which should now be included in the species A. olearius. (nih.gov)
  • strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. (uni-bielefeld.de)
  • Battistoni F, Bartels D, Kaiser O, Reamon-Buettner SM, Hurek T, Reinhold-Hurek B. Physical map of the Azoarcus sp strain BH72 genome based on a bacterial artificial chromosome library as a platform for genome sequencing and functional analysis. (uni-bielefeld.de)
  • This cluster consists of eight genes ( bnsABCDEFGH ) corresponding to 8.1 kb, which are closely related to genes for enzymes involved in anaerobic toluene degradation within the denitrifiers " Aromatoleum aromaticum " EbN1, Azoarcus sp. (ufz.de)
  • Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and Description of Two Species, Azoarcus indigens sp. (wikipedia.org)
  • The taxonomic relationships of Azoarcus and Thauera isolates in the beta-subclass of the Proteobacteria capable of degrading fluoro-, chloro- or bromobenzoate under denitrifying conditions were analysed. (microbiologyresearch.org)
  • A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. (nih.gov)
  • Sensitive detection of a novel class of toluene-degrading denitrifiers, Azoarcus tolulyticus, with small-subunit rRNA primers and probes. (asm.org)
  • Azoarcus tolulyticus is a new class of widely distributed toluene-degrading denitrifiers of potential importance in remediating benzene, toluene, ethylbenzene, and xylene (BTEX)-contaminated environments. (asm.org)
  • Benzylfumaric, benzylmaleic, and Z- and E-phenylitaconic acids: synthesis, characterization, and correlation with a metabolite generated by Azoarcus tolulyticus Tol-4 during anaerobic toluene degradation. (semanticscholar.org)
  • E-Phenylitaconic acid has been isolated as a metabolite generated by Azoarcus tolulyticus Tol-4 along with benzylsuccinic acid during anaerobic degradation of toluene. (semanticscholar.org)
  • Evidence for acetyl coenzyme A and cinnamoyl coenzyme A in the anaerobic toluene mineralization pathway in Azoarcus tolulyticus Tol-4. (semanticscholar.org)
  • Detection of benzylsuccinate in cultures of the toluene-degrading Azoarcus tolulyticus Tol-4 and Thauera aromatica strain T1 suggests that a fumarate addition reaction may also be involved in anaerobic toluene mineralization in these microorganisms ( 7 , 13 ). (asm.org)
  • DNA-DNA hybridization experiments established that strains 2FB2, 2FB6 and 4FB10 belong to the species Azoarcus tolulyticus. (microbiologyresearch.org)
  • Azoarcus is a genus of nitrogen-fixating bacteria. (wikipedia.org)
  • Azoarcus olearius is a species of bacteria. (wikipedia.org)
  • Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. (nih.gov)
  • The genus Azoarcus is comprised of nitrogen-fixing bacteria [ 12 ] and known for degradation of aromatic compounds. (biomedcentral.com)
  • Endophytic diazotrophic bacteria ( Azoarcus spp . (uni-bremen.de)
  • Azospirilla are predominantly surface-colonizing bacteria, whereas A. diazotrophicus, H. seropedicae and Azoarcus sp. (agriville.com)
  • Initial reactions in anaerobic oxidation of m-xylene by the denitrifying bacterium Azoarcus sp. (semanticscholar.org)
  • Draft genome sequence of a nitrate-reducing, o-phthalate degrading bacterium, Azoarcus sp. (uni-konstanz.de)
  • A new study published in Environmental Science & Technology Letters highlights the effectiveness of a bacterium called Azoarcus sp. (labroots.com)
  • Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. (nih.gov)
  • strain PA01 T belongs to the genus Azoarcus, of the family Rhodocyclaceae within the class Betaproteobacteria. (uni-konstanz.de)
  • The analyses of fatty acids and 16S rRNA gene sequencing differentiated strains 2FB2, 2FB6 and 4FB10 as new members of the genus Azoarcus and strains 4FB1, 4FB2, 3CB2, 3CB3 and 3BB1 as new members of the genus Thauera. (microbiologyresearch.org)
  • Benzylsuccinate synthase has been purified from Thauera aromatica strain K172 and Azoarcus sp. (asm.org)
  • Characterization of halobenzoate-degrading, denitrifying Azoarcus and Thauera isolates and description of Thauera chlorobenzoica sp. (microbiologyresearch.org)
  • nov. and Azoarcus toluclasticus sp. (nih.gov)
  • The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus (group 3) and a genomovar of this species (group 4), respectively. (nih.gov)
  • Substrate range of benzylsuccinate synthase from Azoarcus sp. (stanford.edu)
  • In substrate range studies with partially purified benzylsuccinate synthase from denitrifying Azoarcus sp. (stanford.edu)
  • Benzylsuccinate Synthase of Azoarcus sp. (asm.org)
  • In this study, we identified and sequenced the genes encoding benzylsuccinate synthase from Azoarcus sp. (asm.org)
  • Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. (epa.gov)
  • The genome sequence showed high synteny with Azoarcus sp. (nih.gov)
  • Inspection of the complete genome sequence of the Azoarcus sp. (apsnet.org)
  • The genome sequence has shown that Azoarcus has very few mobile genetic elements, or phage-related genes, suggesting its adaptation to a fairly stable, low-stress environment. (wishartlab.com)
  • The genome of Azoarcus olearius DQS-4 T , a N 2 -fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, was sequenced and compared with other Azoarcus strains. (nih.gov)
  • Identification of grass-associated and toluene-degrading diazotrophs, Azoarcus spp. (wikipedia.org)
  • Comparative sequence analysis of the four encoding subunits ( ncrABCD ) showed the gene product to have the closest similarity to the Azoarcus type of benzoyl-CoA reductase. (ufz.de)
  • Other free-living diazotrophs repeatedly detected in association with plant roots, include Acetobacter diazotrophicus, Herbaspirillum seropedicae, Azoarcus spp. (agriville.com)
  • DIGITAL.CSIC: Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. (csic.es)
  • Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. (csic.es)
  • Biochemical studies in Azoarcus sp. (asm.org)
  • Because these properties are poorly understood, a microtiter-plate-based screening of a transcriptional gfp (green fluorescent protein) fusion library of Azoarcus sp. (apsnet.org)
  • The BzdR transcriptional regulator that controls the P(N) promoter responsible for the anaerobic catabolism of benzoate in Azoarcus sp. (csic.es)
  • For details on SnRNP see Azoarcus group I intron . (proteopedia.org)
  • In a previous contribution, we discovered a variant of the Azoarcus group I ribozyme that represents a local peak in the RNA fitness landscape. (royalsocietypublishing.org)
  • Finally, we used NAST to build in missing loops in the crystal structures of the Azoarcus and Twort ribozymes, and to incorporate crystallographic data into the Michel-Westhof model of the T. thermophila group I intron, creating an integrated model of the entire molecule. (simtk.org)